LOCUS       DASTFC010000001         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_346104,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000001 DASTFC010000000
VERSION     DASTFC010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346104"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFO88_00005"
     CDS             <1..208
                     /locus_tag="VFO88_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543933.1"
                     /translation="PQSYLIRIVTLWDSLEAIERSEQSRSQVRESMVATAGASIESVE
                     RAAVVFQDLVEPGPQADPEPEPAG"
     gene            209..1057
                     /locus_tag="VFO88_00010"
     CDS             209..1057
                     /locus_tag="VFO88_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9543934.1"
                     /translation="MRAVVVDAEGVPRLAEVADPAGDGELVDVLACGLCGSDVEKLGP
                     AAAGLVLGHEVVGRTGDGRRVALVHHAPCGECERCRAGHESTCERFRAPTILPGGFAE
                     RALATGGVVELPDAVDDALGTYAEPLGCVLRALERVPREDVIVVGGGFVGRLFAWALR
                     RRGDAVHVVDRDPARSDGPPPGGAAAVVLAAPGGADDALALVEPGGTLLVFADAGPLD
                     LARVYREEVTVAGSRSTTPRHLAEAVELLPELDLPEPLVLPLERFADGLERYRRREAL
                     KVVFTP"
     gene            complement(1047..2390)
                     /locus_tag="VFO88_00015"
     CDS             complement(1047..2390)
                     /locus_tag="VFO88_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9543935.1"
                     /translation="MEREKPLRLDRRQLLVGGGLVAGAAGLGGAAAYALRGDGGPAPA
                     AQPAAAADDDDREFALDGDHTDLTTFVLAPHPRRVREAIERHRAALDASPSLALLEGE
                     PALEEAARAAAAAYLGTSPDLVALTDSTTMGLGLVYAGLGLAEDDEVLTTEHDFYATH
                     EALRLRAERDGIRVERVALYDDPASADAGRIADAVADRITPRTRAVAVTWVHSSTGVK
                     LPLADLAEVVDDANGGRAPEERVLLCVDGVHGFGVEAASPVELGADVFVSGCHKWLFG
                     PRGTGLVWATPEAWERLRPTIPSFSPQAYLAWLEGRAPSGGPPGPFMTPGGYQSFEHR
                     WALPEAFALHEELGRGEVEAHTHGLATRLKEGLAEPAGVRIVTPIAEELSAGLVCCEL
                     DGLGPPDAAFRLRDEHRVIASVTPYATSYLRFGPSIANDEADVDAAVEAVRALTA"
BASE COUNT          291 a          978 c         1046 g          315 t
ORIGIN      
        1 cccccagtcg tacctcatcc ggatcgtcac gttgtgggac tcgctggagg cgatcgaacg
       61 aagcgagcag tcgcggtcgc aggtgcggga gagcatggtc gcgacggcgg gcgcgagcat
      121 cgagtcggtc gagcgcgccg cagtcgtctt ccaggatctc gtcgagccgg ggccgcaggc
      181 cgacccggag cccgagcccg ccggctgatt gcgcgcggtc gttgtcgacg cggagggggt
      241 gccgcggctc gccgaggtcg ccgatccggc cggcgacggc gagctcgtcg acgtgctcgc
      301 gtgcgggctc tgcgggagcg acgtcgagaa gctcgggccg gcggcggccg ggctcgtgct
      361 cggccacgag gtcgtcggac ggacgggcga cgggcggcgc gtcgcgctcg tccaccacgc
      421 gccgtgcggc gagtgcgagc gctgccgcgc cggccacgag tcgacgtgcg agcggttccg
      481 ggcgccgacg atcctgccgg gcgggttcgc ggagcgggcg ctcgcgacgg gcggcgtcgt
      541 ggagctgccg gacgcggtcg acgacgcgct cgggacgtac gcggagccgc tcgggtgcgt
      601 cctgcgggcg ctcgaacggg tgcctcggga agacgtgatc gtcgtcggcg gcgggtttgt
      661 ggggcggctg ttcgcctggg cgctccggcg ccgcggggac gcggtgcacg tggtcgaccg
      721 cgacccggcg cggagcgacg ggccgccgcc cggcggcgcc gcagccgtcg tgctcgcggc
      781 cccgggcggc gcggacgacg cgctcgcgct cgtcgagccc ggcggaaccc tgctcgtgtt
      841 cgccgacgcg gggccgctcg acctcgcgcg cgtgtaccgc gaggaggtca cggtcgccgg
      901 ctcgcgctcc acgacgccgc ggcacctggc ggaggcggtc gagctcctcc ccgagctcga
      961 cctgcctgag ccgctcgtcc tgccgctcga gcggttcgcc gacgggctcg agcgctaccg
     1021 gcggcgggag gcgctcaagg tcgtcttcac gccgtgagcg cgcgcacggc ttcgacggcg
     1081 gcgtcgacgt cggcctcgtc gttcgcgatg ctcgggccga agcgcaggta gctcgtcgcg
     1141 tacggcgtca cgctcgcgat cacgcggtgc tcgtcgcgga ggcggaaggc cgcgtccggc
     1201 gggccgaggc cgtcgagctc gcagcagacg aggcccgcgg agagctcttc ggcgatcggc
     1261 gtcacgatcc gcacgccggc cggctccgcg agcccctcct tcaggcgcgt cgcgagcccg
     1321 tgcgtgtgcg cctcgacctc gccgcggccg agctcctcgt gcagggcgaa cgcctccggg
     1381 agcgcccagc ggtgctcgaa cgactggtag cccccgggcg tcatgaacgg cccgggcggg
     1441 ccgccggagg gcgcgcggcc ctcgagccag gcgaggtacg cctgcgggga gaagctcggg
     1501 atcgtcgggc ggagccgctc ccaggcctcg ggcgtcgccc agacgaggcc cgtgccgcgt
     1561 gggccgaaga gccacttgtg gcagccggag acgaagacgt ccgcgccgag ctcgacgggc
     1621 gacgcggcct ccacgccgaa cccgtggacg ccgtccacgc agaggaggac gcgctcctcg
     1681 ggcgcgcggc cgccgttcgc gtcgtcgacg acctccgcga ggtccgcgag cggcagcttc
     1741 acgccggtgc tcgagtgcac ccacgtgacg gcgacggccc gcgtgcgcgg cgtgatgcgg
     1801 tcggcgaccg cgtccgcgat ccggccggcg tccgctgagg ccgggtcgtc gtagagcgcg
     1861 acgcgctcga cccggatccc gtcccgctcg gcgcgcaggc ggagcgcctc gtgcgtcgcg
     1921 tagaagtcgt gctccgtcgt cagcacctcg tcgtcctcgg cgagcccgag ccccgcgtag
     1981 acgaggccga ggcccatcgt ggtcgagtcg gtcagcgcga cgaggtcggg gctcgtcccg
     2041 aggtaggccg ccgccgccgc acgggcggct tcctcgaggg ccggctcgcc ctcgagaagc
     2101 gccagcgacg ggctcgcgtc gagcgcggcg cggtggcgct cgatcgcctc gcggacgcgg
     2161 cgcggatggg gcgcgagcac gaacgtcgtc agatccgtgt ggtcgccgtc gagcgcgaac
     2221 tcgcggtcgt cgtcgtcggc cgccgcggca ggctgcgcgg ccggcgccgg cccgccgtcg
     2281 ccccgcagcg cataggccgc ggcgccgccc aggcccgccg cgcccgcgac gagcccgccg
     2341 ccgacgagca gctgccggcg atcgagccgg aggggcttct cccgctccat gtcgcaatac
     2401 ggtagtccgg ccgccggggt tccacggcgc cgagcacggc agccgtacga tccgggcgtg
     2461 cgcgcgctcg tcttccacgg ccccggcgac gcccggctgg aggacgtccc ggagccggag
     2521 cccgggcccg gcgacgtgct cgtccaggtc gaggtcgccc tcaccgacgg gacggacgcc
     2581 aaggcctacc ggcgcggcca cccggtgctg ctcggcccgc cgccgagccc
//
LOCUS       DASTFC010000002         6354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_922949,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000002 DASTFC010000000
VERSION     DASTFC010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922949"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFO88_00020"
     CDS             complement(<1..480)
                     /locus_tag="VFO88_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9543936.1"
                     /translation="MRTCVVSDIHANLHALEAVLEAIDGEGADELWCLGDLVGYGPRP
                     NECTRIVRERADVCLVGNHDLGVLGRLDLDLFAADAAASARWTAAELADDARRFLESL
                     RPEGTRAGVALFHASARDAVWEYVMTEDVAAESLVSSPEALTLVGHSHLALEFSLTGP
                     "
     gene            451..1755
                     /locus_tag="VFO88_00025"
     CDS             451..1755
                     /locus_tag="VFO88_00025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9543937.1"
                     /translation="MDVGHDAGAHGGATLAKAAGGIALGCLWIAAAWLLLDTRTPAGL
                     VLPDLDPDAYFPAEHLERADRFRTVTRALWALGVLVQLAVLALVVWQARRLAAWLSPR
                     ARGRVRTGVALALLALVATTVAGLPLGVFRHSWNRRYGLSEQGYLAWLGDRAISFGLT
                     ALLLTIAVAGALWLAGRLGSRWWVAAAPALVALGVGYVVIQPVVVQPLFNRFEPLADA
                     QLAREVERLGDEIGVQVESVQVADASRRTTTANAYVAGLGPTRRVVFYDTALDGRFTD
                     DELASLAAHELAHVERNHLWKGLAWFALLALPGLALVAWGTERRGGLRDPALVPLALL
                     LALVAILLTQPVANAVSRVYEAEADWIALEATDDPESAIGLTVRFSLTGLGVPDPPAW
                     ARIMLSTHPPGIDRIAMAEAYASGTWQTASRFTPSGSSTNAP"
     gene            complement(1686..2051)
                     /locus_tag="VFO88_00030"
     CDS             complement(1686..2051)
                     /locus_tag="VFO88_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9543938.1"
                     /translation="MIDHVKLFVRDPAASRAFYEAALEPLGYRVIIDRGDGAVGMGRD
                     RPDLWLAHGERTTVTHLALRADDRATVDAFHAAALAAGGRDNGAPGPRPQYHPTYYGA
                     FVLDPDGVNLEAVCHVPEA"
     gene            2091..3611
                     /locus_tag="VFO88_00035"
     CDS             2091..3611
                     /locus_tag="VFO88_00035"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9543939.1"
                     /translation="MTGAFAVDRLAEELARAAGAPVELERPREAAHGDYATNVALRLA
                     PTRGRAPRELAEEIAGAATESALVERAEVAGPGFVNLWVTSDWYRDAVVEIAERPGEY
                     GGGFASPRERVQVEMVSANPTGPITVASARNGAYGDAIARVLAFAGNEVEREYYYNDA
                     GAQMDRFRASVEAVRRGEEPPEDGYRGAYLDELAALDGDPVPLMLERIEESLERFRIH
                     FDSWARQSELERRLPEYLARLDTYEKDGALWARSSAHGDDEDRVLVRSEGGAPTYRAA
                     DVVYLVDKLERGFDRAIYVLGADHHGTRNWYAAVARMLGYDPARVEVLLYQLVHLTRG
                     GEQTKMSKRRGDVVFLDDFVDEVGVDAARWYLVSRGHDQTIEIDVDLARERSEKNPVY
                     YVQYAHARIAGILRNAGDARAELEPGVEPEREERELVKRLAEFPGVVAEAAERRGPHA
                     LPNYAIRVADDFHRFYHHHRVLESEAQGFRLALCRATQAVIARSLDLIGVEAPERM"
     gene            3699..4595
                     /locus_tag="VFO88_00040"
     CDS             3699..4595
                     /locus_tag="VFO88_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9543940.1"
                     /translation="MRWNLLLAGIAASWGFVSVIASGVDLPAEVLVFWRCLLAAAALP
                     AMVYVLRGRLPFRLPRHRVRVLALGALLALHWVLFFAAIKASSVAVAILTVYTAPIFV
                     ALLAPRFLPEARSRVALVALAVSAPGLVLIALAGDAGEPPSAAAIALGLGAAITYAFL
                     IIGVKRITNEVSPFAFAFWQYAVVSVALAPFLLTADRVLPAGGEWAAVIVLGIVLTAA
                     TGALYIWNLRHVKAQAAGLLSYVEPVSAALLAWAVLGEALGWAVVVGGAAVLAGGAIV
                     VLREEPDAPPREAPPLREPEPV"
     gene            4606..5592
                     /gene="glpX"
                     /locus_tag="VFO88_00045"
     CDS             4606..5592
                     /gene="glpX"
                     /locus_tag="VFO88_00045"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET9543941.1"
                     /translation="MPTIPDRNLALELVRVTEAAALGAGRWVGRGDKIAADQAAVDGM
                     RAVLDTVAMSGVVVIGEGEKDEAPMLYNGEEVGNGQGPEVDVAVDPLEGTRLTATGQP
                     NAIAVIAVAERGTMFFPGAAVYMDKIAAGAEVADAIDLDASPQDNVRAAAGAKGVKPS
                     EISVVVLDRDRNQELIAGLRDVGARVVLITDGDVAPAIAAANPRRPVDLMMGIGGTPE
                     GVIAAAAVKALGGSVQGRLWPRSDEERRAIVDAGLDPDAKLTTDDLVGGDNLFVAATG
                     VTDGALLDGVRYTRDGAETDSIVIRSRSGTVRRVQAWHELDKLARFSGREYR"
     gene            5647..>6354
                     /locus_tag="VFO88_00050"
     CDS             5647..>6354
                     /locus_tag="VFO88_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543942.1"
                     /translation="MGVPEHRAPTASTRRLRPVTERVLARIPGPRAAGIAAWALVPWL
                     NAGANLLLPAELRTEVWEQSGALIVLNYTALSAAVVLTVWGADRIARRVEELPARTGT
                     LRVDASERFAEINGVAGPLLAAVATAAAFALVTLADAGWGPAALRAPTWFVLGVAFWG
                     FLWTYGALQLGLDRLGREPLGRDAAAADPTLGLAPVGRLAFTGLWLLLAWLVPVMLTA
                     LEDVVAVAVGAAVLAVAL"
BASE COUNT          727 a         2386 c         2379 g          862 t
ORIGIN      
        1 gggcccggtg agcgagaact cgagcgcgag atggctgtgc ccgacgagcg tgagcgcctc
       61 cggcgagctg acgagcgact cggcggccac gtcctcggtc atgacgtact cccagacggc
      121 gtcccgcgcg ctcgcgtgga agagcgcgac gccggcgcgc gtcccctcgg gccggaggga
      181 ctcgaggaac cggcgcgcgt cgtccgcgag ctcggccgcg gtccagcggg cgctcgcggc
      241 cgcgtcggcg gcgaagaggt cgaggtcgag ccggccgagc acgccgaggt cgtggttccc
      301 gacgaggcag acgtcggcgc gctcgcgcac gatccgcgtg cactcgttcg gccgcggccc
      361 gtaccctacg aggtccccga ggcaccagag ctcgtcggcg ccctcgccgt cgatcgcctc
      421 gaggacggcc tcgagggcgt ggaggttcgc gtggatgtcg gacacgacgc aggtgcgcac
      481 ggcggcgcaa cgctagcgaa ggccgcgggc gggatcgccc tcggctgcct ctggatcgcg
      541 gccgcctggc tcctcctcga cacccggacg cccgccgggc tcgtcctgcc ggacctcgac
      601 ccggacgcgt acttcccggc cgagcacctc gagcgcgccg accggttccg cacggtgacc
      661 cgcgcgctct gggccctcgg cgtcctcgtc cagctcgccg tgctcgcgct cgtcgtctgg
      721 caggcgcgac ggctggccgc gtggctctct ccgagggcgc ggggccgcgt gcgaaccggc
      781 gtcgcgctcg cgctcctcgc gctcgtggcg acgaccgtcg ccggcctgcc gctcggcgtc
      841 ttccgccact cgtggaaccg ccggtacggg ctctccgagc agggctacct cgcgtggctc
      901 ggcgaccgtg cgatctcgtt cgggctgacc gcgctcctgc tcacgatcgc cgtcgcggga
      961 gccctctggc tcgccgggcg gctcggctcg cggtggtggg tcgcggccgc gcccgcgctc
     1021 gtcgcgctcg gcgtcggcta cgtcgtgatc cagccggtcg tcgtccagcc gctcttcaac
     1081 cggttcgagc cgcttgcgga cgcgcagctc gcgcgcgagg tggagcggct cggggacgag
     1141 atcggcgtgc aggtcgagag cgtccaggtg gccgacgcga gccggcgcac gacgaccgcg
     1201 aacgcgtacg tcgcgggtct cggcccgacg cggcgggtcg tcttctacga cacggcgctc
     1261 gacgggcgct tcaccgacga cgagctcgcc tcgctcgccg cccacgagct cgcccacgtc
     1321 gagcggaacc acctctggaa ggggctcgcg tggttcgcgc tcctcgcgct gccggggctc
     1381 gcgctcgtcg cgtgggggac ggagcggcgc ggcgggctcc gcgacccggc gctcgtgccg
     1441 ctcgcgctgc tcctcgcgct cgtcgcgatc ctcctgacgc agccggtcgc gaacgccgtc
     1501 tcgcgcgtct acgaggcgga ggccgactgg atcgcgctcg aggcgaccga cgacccggag
     1561 agcgcgatcg ggctcaccgt ccggttctcg ctcaccgggc tcggcgtccc cgacccgccc
     1621 gcctgggcgc ggatcatgct ctcgacgcac ccgcccggga tcgaccggat cgcgatggcg
     1681 gaggcctacg cctccggcac gtggcagacc gcctcgaggt tcacgccgtc cgggtcgagc
     1741 acgaacgcgc cgtagtacgt cgggtggtac tgcggccgcg ggccgggcgc cccgttgtcg
     1801 cgcccgccgg ccgcgagcgc ggcggcgtgg aaggcgtcga cggtcgcacg atcgtccgcc
     1861 cgcagggcga gatgcgtcac ggtcgtgcgc tcgccgtggg cgagccagag atccggccgg
     1921 tcccggccca tgccgaccgc gccgtcgccg cggtcgatga tgacgcggta gccgagcggc
     1981 tcgagcgccg cctcgtagaa cgcgcggctc gcggcggggt cgcgcacgaa gagcttcacg
     2041 tggtcgatca tgcgacgagc ctaccgcggc cgcctcgtac gatgtgcgcc gtgacgggag
     2101 cgttcgccgt ggatcggttg gccgaggagc tggcgcgcgc cgccggtgcg ccggtcgagc
     2161 tcgagcgccc gcgggaggcg gcgcacggcg actacgcgac gaacgtcgcg ctccggctgg
     2221 cgccgacccg gggccgggcg ccgcgcgagc tcgccgagga gatcgcgggc gccgcgacgg
     2281 agagcgcgct cgtcgagcga gccgaggtcg ctggccccgg attcgtgaac ctgtgggtga
     2341 cgagcgactg gtaccgcgac gccgtcgtcg agatcgcgga gcggcccggg gagtacggcg
     2401 gcgggttcgc gtcgccgcgc gagcgcgtcc aggtcgagat ggtctccgcg aacccgaccg
     2461 gcccgatcac ggtcgcctcg gcgcggaacg gcgcgtacgg cgacgcgatc gcgcgcgtgc
     2521 tcgcgttcgc ggggaacgag gtcgagcgcg agtactacta caacgacgcg ggcgcccaga
     2581 tggatcggtt ccgcgcctcg gtcgaggccg tgcggcgcgg cgaggagccg cccgaggacg
     2641 gctaccgcgg cgcctatctc gacgagctcg cggcgctcga cggcgacccg gtcccgctca
     2701 tgctcgagcg gatcgaggag tcgctcgagc ggttccggat ccatttcgac tcctgggcgc
     2761 ggcagagcga gctcgagcgc cggctccccg agtacctcgc gcgcctcgac acgtacgaga
     2821 aggacggggc gctctgggcc cgctcgtccg cgcacggcga cgacgaggac cgagtgctcg
     2881 tccgctcgga gggcggcgcc ccgacgtaca gggccgcgga cgtcgtctac ctcgtggaca
     2941 agctcgagcg cggcttcgac cgggcgatct acgtcctcgg cgccgaccat cacgggacgc
     3001 ggaactggta cgcggccgtc gcgcggatgc tcgggtacga cccggcgcgc gtcgaggtgc
     3061 tcctgtacca gctcgtccac ctgacgcggg gcggcgagca gacgaagatg tcgaaacggc
     3121 gcggggacgt ggtcttcctg gacgacttcg tggacgaggt cggtgtcgac gcggcgcgct
     3181 ggtacctcgt ctcgcgcggc cacgaccaga cgatcgagat cgacgtcgac ctcgcccgcg
     3241 agcggtcgga gaagaacccc gtctactacg tccagtacgc ccacgcgcgg atcgcgggga
     3301 tcctgcgcaa cgcgggcgac gcgcgcgccg agctcgagcc cggggtcgag cccgagcggg
     3361 aggagcgcga gctcgtgaag cggctcgccg agttccccgg cgtcgtcgcc gaggcggccg
     3421 agcgtcgcgg gccccacgcg ctcccgaact acgccatccg cgtcgcggac gacttccacc
     3481 gcttctatca ccaccaccgc gtgctcgaga gcgaggccca gggcttccgg ctcgcgctct
     3541 gccgcgcgac gcaggccgtg atcgcccgga gcctcgacct gatcggcgtc gaggcccccg
     3601 agcggatgta gcccgttccg gccgcgtcgc aggcggccat tgtccggtgc caggcaccgg
     3661 ccacggccgc cggggacacg cggctacgat cggccgccgt gcggtggaac ctcctcctgg
     3721 ccgggatcgc cgcctcgtgg ggcttcgtct ccgtgatcgc gagcggggtc gacctgcccg
     3781 cggaggtgct cgtgttctgg cgctgcctcc tcgcggcggc ggcgcttccg gcgatggtct
     3841 acgtcctgcg cgggcggctg cccttccggc tcccgcgcca ccgcgtgcgc gtcctcgccc
     3901 tcggcgcgct gctcgcgctc cactgggtgc tcttcttcgc cgcgatcaag gcgtcgtcgg
     3961 tcgcggtcgc gatcctgacc gtctacacgg cgccgatctt cgtcgcgctc ctcgcgccgc
     4021 gcttcctgcc ggaggcccgc tcgcgggtcg cgctcgtcgc gctcgccgtg tcggcgccgg
     4081 gcctcgtcct catcgcgctc gcgggcgacg cgggggagcc gcccagcgcg gccgcgatcg
     4141 cgctcggcct cggcgcggcg atcacgtacg cgttcctcat catcggcgtg aagcggatca
     4201 cgaacgaggt ctccccgttc gcgttcgcct tctggcagta cgcggtcgtc tccgtcgcgc
     4261 tcgccccgtt cctcctcacc gccgaccgcg tgctcccggc cggcggcgag tgggcggcgg
     4321 tgatcgtgct cgggatcgtc ctcaccgccg cgacgggcgc gctctacatc tggaacctcc
     4381 gccacgtgaa ggcgcaggcg gccggcctgc tctcgtacgt cgagcccgtg tccgcggcgc
     4441 tcctcgcgtg ggcggtgctc ggcgaggcgc tcggctgggc cgtcgtcgtc ggcggcgcgg
     4501 ccgtcctcgc gggcggcgcg atcgtcgtcc tgcgcgagga gccggacgca ccgccgcgcg
     4561 aggcgccccc gctgcgcgag cccgagcccg tctaggatcg tgcccgtgcc gacgatcccc
     4621 gaccggaacc tcgcgctcga gctcgtccgc gtcaccgagg cggcggcgct cggcgcgggc
     4681 cgctgggtcg ggcgcgggga caagatcgcc gcggaccagg ccgccgtcga cgggatgcgg
     4741 gccgtcctcg acacggtcgc catgtccggc gtggtcgtga tcggcgaggg ggagaaggac
     4801 gaggcgccga tgctctacaa cggcgaggag gtcggcaacg gacaggggcc ggaggtcgac
     4861 gtcgccgtcg acccgctcga ggggacgcgg ctcaccgcga ccggccagcc gaacgcgatc
     4921 gccgtcatcg ccgtcgcgga gcgcgggacg atgttcttcc cgggcgcggc cgtgtacatg
     4981 gacaagatcg ccgccggcgc cgaggtcgcg gacgcgatcg atctcgacgc gagcccgcag
     5041 gacaacgtcc gcgccgccgc cggggcgaag ggcgtgaagc cgagcgagat ctcggtcgtc
     5101 gtcctcgacc gcgaccggaa tcaggagctc atcgccggcc tgcgggacgt cggcgcccgc
     5161 gtcgtcctca tcacggacgg cgacgtcgcg cctgcgatcg cggccgcgaa cccgcgccgg
     5221 ccggtcgacc tgatgatggg gatcggcggg acgccggagg gcgtgatcgc ggcggcggcc
     5281 gtgaaggcgc ttggcggctc ggtgcagggc cgcctctggc cgcgctcgga cgaggagcgc
     5341 cgcgcgatcg tggacgccgg gctcgacccg gacgcgaagc tcacgacgga cgacctcgtc
     5401 ggcggcgaca acctgttcgt cgccgcgacg ggcgtcacgg acggcgcgct tctcgacggc
     5461 gtcaggtaca cgcgcgacgg cgccgagacg gactcgatcg tcatccgttc ccgctccggc
     5521 accgttcggc gcgtccaggc ctggcacgag ctcgacaagc tcgcgcgctt ctccggccgc
     5581 gaataccgct gacgccgcgc ttgcgggaac acgcccgcgg cggcacgatc acgtgtcacc
     5641 cgcacgatgg gcgttcccga gcaccgcgca ccgaccgcgt cgacgcgccg cctccggccc
     5701 gtcaccgagc gcgtcctcgc gcgcattccc ggcccgcggg ccgcggggat cgccgcctgg
     5761 gcgcttgtcc cctggctgaa cgcaggggcg aacctgctcc ttccggccga gctgcggacg
     5821 gaggtgtggg agcagagcgg cgcgctcatc gtcctgaact acaccgcgct gagcgcagcc
     5881 gtcgtgctca cggtgtgggg agccgatcgc atcgcccggc gcgtcgagga gctgccggcg
     5941 aggacgggca ccctgcgcgt ggacgcgtcc gagcggttcg ccgagatcaa cggcgtcgcc
     6001 gggccgctcc tcgcggccgt ggcgaccgcg gcggccttcg cgctcgtcac gctcgccgac
     6061 gccgggtggg ggcccgccgc ccttcgcgcg cccacgtggt tcgtgctcgg cgtcgcgttc
     6121 tggggcttcc tctggacgta cggagcgctc cagctcggcc tcgaccgcct cggccgcgag
     6181 ccgctcggcc gggacgccgc cgccgccgat ccgacgctcg ggctcgcgcc ggtcggccgg
     6241 ctcgccttca ccgggctctg gctcctgctc gcgtggctcg tcccggtcat gctcaccgcg
     6301 ctcgaggacg tcgtcgcggt cgcggtcggc gcggccgtcc tcgccgtcgc cctc
//
LOCUS       DASTFC010000003         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1565674,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000003 DASTFC010000000
VERSION     DASTFC010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565674"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFO88_00055"
     CDS             <1..324
                     /locus_tag="VFO88_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9543943.1"
                     /translation="RVHVEGEPAPVPRAIDLSAYRIVQEGLTNALKHARAGGADVTVR
                     YAPAELVLEVRDDGAGAAESDGLGHGLVGIRERVKIYGGDMTAGPAPGGGFVLRARLP
                     FEGRS"
     gene            321..986
                     /locus_tag="VFO88_00060"
     CDS             321..986
                     /locus_tag="VFO88_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9543944.1"
                     /translation="MSIRILVADDQSMVRAGFRMLLGGEPDIEVVAEASNGLEAVDKA
                     ARFHPNVVLMDIRMPELDGLEATRRILAADANARVLVLTTFDLDEYVYEALRAGASGF
                     VLKDDPPEQLLAAIRIVAAGEALLSPAITKRVIERFTRLPRPEAPRGLEELTERELDV
                     FRLVARGLSNAEIGKELFISETTVKTHITHVLRKLGLRDRVQAVVLAHEAALFEADER
                     PAG"
     gene            1245..1763
                     /locus_tag="VFO88_00065"
     CDS             1245..1763
                     /locus_tag="VFO88_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9543945.1"
                     /translation="MRERDDSVDTITAQWRRERPDLDSSPMEVVGRITRLADLMRREQ
                     DRVFAEFGLAGGDFDVLATLRRSKPPHRLAPGELSRSTMVTTGGMTKRLDRLEARGLV
                     RREADPRDRRGRLIALTDEGRDLVDRTLEAHLENEERMLSSLPAARREELADLLRGLL
                     LALDVRRGPEPA"
     gene            1848..2207
                     /locus_tag="VFO88_00070"
     CDS             1848..2207
                     /locus_tag="VFO88_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9543946.1"
                     /translation="MEKVNLEAALATFDEAWSPRIVGRYNGNKVFLAKAEGDFVWHSH
                     PETDDLFLVLEGRLTIDLPDGAVELGPGELFVVPRGVEHRPRSDEGASVLVIEPEGTP
                     NTGDEATAATPVDLTAR"
     gene            complement(2204..2596)
                     /locus_tag="VFO88_00075"
     CDS             complement(2204..2596)
                     /locus_tag="VFO88_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9543947.1"
                     /translation="MSDHTILNLRGVEDSAPGFGFDETMESRFATRPLGGSIVGLALE
                     TIKPGKRVPFGHRHGDQEEVYVVVRGGGRVKIEDEIHDVRQWDAIRVGPGTTRNFEAG
                     PDGLEILAFGAPVSVGNDAEILPGWWSD"
     gene            complement(2690..>3091)
                     /locus_tag="VFO88_00080"
     CDS             complement(2690..>3091)
                     /locus_tag="VFO88_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9543948.1"
                     /translation="GGVAEEKLWMDPVTENPAVGATEVWEMYNTTGDAHPMHIHEVVF
                     EVVNREGLVLDDDDEVVEPIELDGNVMPPEPWETGFKDTVTAYPGQVTRVRARFSTPG
                     QFVWHCHIVEHEDNEMMRPYRIGPEQPGQPE"
BASE COUNT          431 a         1123 c         1097 g          440 t
ORIGIN      
        1 cgcgtccacg tcgagggcga gccggccccg gtgccgcgcg cgatcgacct ctccgcgtac
       61 cggatcgtcc aggaggggct cacgaacgcg ctcaagcacg cgcgcgcagg cggggccgac
      121 gtgaccgtcc gctacgcgcc cgcggagctc gtgctcgagg tgcgcgacga cggggccggc
      181 gccgccgaga gcgacgggct cggccacggg ctcgtcggga tccgcgagcg cgtgaagatc
      241 tacggcggcg acatgaccgc ggggcccgcg cccgggggcg gcttcgtcct gcgcgcgcgg
      301 ctgccgttcg agggccggtc gtgagcatcc gcatcctcgt cgcggacgac cagtcgatgg
      361 tgcgcgccgg cttccggatg ctgctcggcg gcgagcccga catcgaggtc gtcgccgagg
      421 cgtcgaacgg cctcgaggcg gtcgacaagg cggcgcgctt ccacccgaac gtcgtgctca
      481 tggacatccg gatgcccgag ctcgacgggc tcgaggcgac gcgccgcatc ctcgcggccg
      541 acgcgaacgc gcgcgtgctc gtcctcacca cgttcgacct ggacgagtac gtctacgagg
      601 cgctgcgcgc cggcgcgagc gggttcgtgc tgaaggacga cccgccggag cagctgctcg
      661 cggcgatccg gatcgtcgcc gccggcgagg cgctgctctc gcccgcgatc accaaacggg
      721 tgatcgagcg cttcacgcgt ctgccgcgcc ccgaggcgcc ccgcggcctg gaggagctga
      781 cggagcgcga gctcgacgtc ttccgcctcg tcgcccgcgg gctctcgaac gccgagatcg
      841 gcaaggagct cttcatcagc gagacgaccg tgaagacgca catcacgcac gtcctgcgca
      901 agctcggcct gcgcgaccgc gtccaggcgg tcgtcctcgc ccacgaggcg gccctcttcg
      961 aggcggacga gcggcccgcc ggctagcgcc tccgttcagg tcacgagcat ggctccgccc
     1021 gcaggggccg aaggcgaagg acgcggcgcc gcagcgccgg caggaggcgc cgtgaccgag
     1081 tgggcgcctt catgcccgcg cgcgctcgag cacggcggtc gagggtctcg aactgggtga
     1141 cgtgcgggta cggcatcgtg tctccttttc cggttagctt gcagaaaaga tacttactag
     1201 aaagctttct gtcaagctgc tttcccggca ggtacgatgc tcgcgtgcgt gagagggacg
     1261 actcggtcga cacgatcacc gcgcaatggc gccgggagcg gccggacctc gactcgagcc
     1321 ccatggaggt cgtgggccgg atcacgcgcc tcgcggacct gatgcgacgc gagcaagacc
     1381 gcgtcttcgc cgagttcggg ctcgccgggg gcgatttcga cgtgctcgcc acgctccgcc
     1441 gctcgaagcc gccccaccgg ctcgcgcccg gcgagctcag ccgctcgacg atggtgacga
     1501 ccggcggcat gacgaagcgt ctcgaccgcc tggaggcgcg cgggctcgtc cggcgcgagg
     1561 cggatccgcg cgaccggcgg gggaggttga tcgcgctgac ggacgagggg cgcgacctcg
     1621 tcgaccggac gctcgaggcg catctcgaga acgaggagcg gatgctctcc tcgctcccgg
     1681 cggcgcggcg cgaggagctt gcggatctcc tccgcgggct gctgctcgcg ctcgacgtgc
     1741 gccggggccc cgagccggcc tgacccgagc ggatccctca gggattcccg agccgcggac
     1801 ggtcgttttg acggatcgtc gagcacgacg acaggaggtc acgcgagatg gagaaggtga
     1861 acctggaggc ggccctcgcg acgttcgacg aggcctggtc gccgcggatc gtggggcgct
     1921 acaacggcaa caaggtcttc ctcgcgaagg cggagggcga cttcgtgtgg cactcccatc
     1981 ccgagaccga cgacctcttc ctcgttctcg aggggcgcct cacgatcgac ctgcccgacg
     2041 gcgcggtcga gctcgggccc ggcgagctct tcgtcgtgcc acgcggcgtc gagcaccggc
     2101 cccgctcgga cgagggcgcg tccgtcctcg tgatcgagcc ggagggaacg ccgaacaccg
     2161 gcgacgaggc caccgcggcg acgcccgtcg acctgacggc ccgctagtcg ctccaccagc
     2221 cgggcaggat ctccgcgtcg ttcccgacgg agaccggcgc gccgaaggcg aggatctcga
     2281 gcccgtcggg gcccgcctcg aagttgcggg tcgtcccggg gccgacgcgg atcgcgtccc
     2341 actgccgcac gtcatggatc tcgtcctcga tcttcacgcg gccgccgccg cggacgacga
     2401 cgtagacctc ctcctggtcg ccgtggcggt ggccgaacgg gacgcgcttg ccgggcttga
     2461 tcgtctcgag cgcgaggccg acgatcgacc cgccgagcgg gcgggtggcg aaccgcgact
     2521 ccatcgtctc gtcgaagccg aagcccggcg cggagtcctc gaccccccgc aggttgagaa
     2581 tcgtgtggtc gctcatcggc ggatcatgcc agcccctcga cacccgcccc gcacggggtc
     2641 acgctcgtgg ggctcgcggt cgagccccac gagcgtccgc cgcgccaacc tattccggct
     2701 gaccgggctg ctcgggcccg atccggtacg gccgcatcat ctcgttgtcc tcgtgctcca
     2761 cgatgtggca gtgccagacg aactggccgg gggtcgagaa ccgcgccctc acgcgcgtga
     2821 cctgcccggg gtacgcggtg accgtgtcct tgaacccggt ctcccacggc tcgggcggca
     2881 tgacgttccc gtcgagctcg atcggctcga cgacctcgtc gtcgtcgtcc aggacgagcc
     2941 cctcccggtt cacgacctcg aacacgacct cgtggatgtg catcgggtgc gcgtccccgg
     3001 tcgtgttgta catctcccag acctcggtcg cgccgaccgc gggattctcg gtgaccggat
     3061 ccatccagag cttctcctcc gccactcccc c
//
LOCUS       DASTFC010000004         6459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_758138,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000004 DASTFC010000000
VERSION     DASTFC010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758138"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFO88_00085"
     CDS             complement(<1..444)
                     /locus_tag="VFO88_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9543949.1"
                     /translation="MASETHPRVRRATAADAPVFGRLLQAFNEEYDEPTPSADEIAER
                     AAPLIASGEVTVLFVGDGPEGFAELRFRPSLYSGGLDAYLEELYVVPERRGRGLGRAL
                     LEAAMDYARARGADRIDLNTSEDDVAARALYERTGFTNREGSPDGP"
     gene            complement(495..1115)
                     /locus_tag="VFO88_00090"
     CDS             complement(495..1115)
                     /locus_tag="VFO88_00090"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9543950.1"
                     /translation="MQSRWETGRTEAFSDGVFAIAITLLVLEIGVPADAFDDLWRGIV
                     DEWPSYLGYATSFFTIGGIWLAHHGVFRRLRYADHSVMRINLLLLMAVAFLPFPTKLV
                     AEAIHDESAERAAAIFYGASLLAIALLFSALWGAVGRNRELLKPEVTDAEVTSILIAS
                     SPSIGFYAAAIALAVVAPQVAALAYLVIAVVGVLRARGDQTVAAPA"
     gene            complement(1229..2896)
                     /locus_tag="VFO88_00095"
     CDS             complement(1229..2896)
                     /locus_tag="VFO88_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543951.1"
                     /translation="MRRLTSVLVFVLALCVAGPAAASPTPTPPQDRNWCRQVPPAANW
                     SVKNVEVLCRIDTVRHTWNADGTLAADPSTPSGYRETLTQTISINFIDYGNPREDQPL
                     TAATRDVMFVSGRFGLKSYDMTVPTRPLLLDWLGDGIGDPFLEDGNPGNGSQAPRRGT
                     LDDMWENEDMDVDADRKLVFLSRDPRAYGGSTGNPASRSGIYVVDVRDPAALRVITFV
                     ETPGGHTTTCVNDCNWLWTGGPAPNTDQAAMGWAGRPIFVTDMRNPHKPSVYPEPVDL
                     ARNDGRTDYAHDVQVDDMGIAWVSGRGGVRGYHTKGLHYDPTLGAARVATAWNPVPYA
                     GGGFEETVAPTRTSSRFMHNAWRPIGGDAPDPSYGNLIYATEEEFAGGSFPNGCANDG
                     PLFISSLDGSYDGQAWRSTPTEKFRLRTVATWHPFGKEGSSNSNDCSAHYFQIENRIL
                     SMSFYSQGSRFLDISDPTEPTEIAYFRPGTGTWATYPHKGLYYSADRNGVYVLRLTVG
                     PGDGARMATTSAMHAPAAEADPEEEALAARERPHGGHCTRLLPDFEL"
     gene            complement(3045..5072)
                     /locus_tag="VFO88_00100"
     CDS             complement(3045..5072)
                     /locus_tag="VFO88_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9543952.1"
                     /translation="MERNTTRAFTPARIVALALVALAVLALGYLRFAPEDGPVSVPAG
                     AQAGDIVLEPCDYETEDGTYAADCGTLVVPENRADPDSRLIALPVTRIKAETDDPGEP
                     VFYLEGGPGGTNMEFPGASRFAGDRDVVLVGYRGVDGSERLDCPEVESALAHSTDYLG
                     DASFSAYEGAFRACASRLADEGVDVSSYGLVQQVDDLEDARKALGYERIDLLSESAGT
                     RTALIYAWRYPESIHRSVLVAANPPGHFLWDAETTDEQIARYAKLCADDASCSERTDD
                     LAASLARTNEDIPDRWAFLPIQESSVRIASFFGLMESTSASPVAYGPQTIDSWLSAAE
                     GDPSGFWFASLAGELIFPKMFVWGQYAAAGRVDAAASREYFAGGVDPAANLGTAASAY
                     GWGGGRLADGWPAAHEENRYSRMRTSEVETLVISGELDGSTPPQVATAELMPYLPNGE
                     QVVLRELGHTADFWAEQPEASSRLVNTYLASGRVDASLYERGDVDFSPPLTQVRMAKI
                     LLGLMLALVVVAAGALLAMARRVHRRGGFGRKAAAVLRSVFPLVLGLGGWFAGVLVVL
                     TVMPGVPLDDELLAGFSIGVPIGLGVYLAWVQRAWSAAVKATGLAAATSGALVGAWLG
                     FNTTEGLVALLTTIIGAGAGANLILLGLDIAWDRQARDRAAAERLEPHPAS"
     gene            complement(5262..>6459)
                     /locus_tag="VFO88_00105"
     CDS             complement(5262..>6459)
                     /locus_tag="VFO88_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9543953.1"
                     /translation="RVEKGKIPFVHRLRRPSGDSRVWGAILDRVLRRPLVSAVASAGV
                     LVVLALPALGMHTAFPGISSLPRDIPIMQTYDRVQAAFPGGPLPAVVAVEADDVTSPA
                     FEAALADFRERALATGQVEEPIQVQANDEGTVALISVPLVGTGTDDASTAALMTLRED
                     VIPATLGAVDGAEVAVGGEAASSHDFSEVVKDRAPIVFAFVLLLAFGLLLVAFRSVVI
                     AAKAVVLNLLSVAAAYGLLVVVFQWGWGESLLGFESTGAITAWLPLFMFVVLFGLSMD
                     YHVFILSRIREAYDRGLDTDAAVTHGIKTTAGVVSAAAIVMVAVFSIFATLSTIEMKQ
                     AGVGLAAAILIDATIVRAVLLPSAMKLLGERNWYLPRWLEWLPKVSHETEAPAPPVAA
                     PSPAGS"
BASE COUNT          929 a         2293 c         2378 g          859 t
ORIGIN      
        1 gggcccgtcg ggcgagccct cgcggttcgt gaagccggtg cgctcgtaga gcgcgcgggc
       61 ggccacgtcg tcctcgctcg tgttcaggtc gatccggtcg gcgccgcggg cgcgcgcgta
      121 gtccatcgcc gcctcgagga gcgcccgccc gagcccccgg ccgcggcgct cgggaacgac
      181 gtagagctcc tcgaggtacg cgtcgagccc gccgctgtag agcgacgggc ggaaccggag
      241 ctcggcgaag ccctccggcc cgtctccgac gaagagcacc gtgacctcgc cgcttgcgat
      301 gagcggcgcc gcccgctcgg cgatctcgtc ggccgagggc gtcggctcgt cgtattcctc
      361 gttgaacgcc tgcaacagcc ggccgaagac gggggcgtcc gcggccgttg cgcgccggac
      421 ccgagggtgc gtctcgctcg ccattcccgg cgactctacg cacgagagcc ggtccgagag
      481 gcgacccggc cgcgtcaggc gggcgcggcg acggtctggt cgccccgcgc ccgcagaacg
      541 ccgacgacgg cgatcacgag gtaggcgagc gccgcgacct gcggcgcgac gacggcgagc
      601 gcgatcgccg cggcgtagaa gccgatgctc ggcgacgagg cgatgaggat cgaggtgacc
      661 tccgcgtcgg tcacctccgg cttcaggagc tcccggttcc gcccgaccgc gccccagagg
      721 gcgctgaaga ggagcgcgat cgcgaggagc gacgcgccgt agaagatcgc cgcggcgcgc
      781 tcggcgctct cgtcgtggat cgcctcggcg acgagcttcg tcgggaacgg caggaacgcg
      841 accgccatga gcaggagcag gttgatccgc atcacgctgt ggtcggcgta gcgcagccgc
      901 cggaagacgc cgtgatgggc gagccagatg cctccgatcg tgaaaaagct cgtcgcgtac
      961 ccgaggtagg acggccactc gtccacgatg ccgcgccaga ggtcgtcgaa ggcgtcggcc
     1021 ggcacgccga tctcgagcac gaggagcgtg atcgcgatgg cgaacacgcc gtcgctgaac
     1081 gcctccgtcc ggcccgtctc ccaccggctc tgcacgccga ggaggttcga cgctcatgcg
     1141 ccgcgtcctg tcccgggcgc cctagtgccg ccggtcaggc acgagggccc cacgtacgcg
     1201 gggccctcgt gcgcgtcttc gaatgggcct acagctcgaa gtccggaagg agcctcgtgc
     1261 agtggccgcc gtgcgggcgc tcccgcgccg cgagcgcctc ctcctcgggg tcggcttccg
     1321 cagcgggcgc gtgcatcgcg ctcgtcgtcg ccatccgcgc gccgtcgccc ggcccgacgg
     1381 tgagccgcag gacgtagacg ccgttccggt cggccgagta gtagaggccc ttgtgcggat
     1441 acgtggccca cgtgcccgtc ccgggccgga agtacgcgat ctccgtcggc tccgtcgggt
     1501 cggagatgtc gaggaaccgc gagccctggc tgtagaacga catcgacagg atccggttct
     1561 cgatctggaa gtagtgggcc gagcagtcgt tcgagttcga ggagccctcc ttgccgaacg
     1621 ggtgccacgt ggcgaccgtc cggaggcgga acttctcggt cggcgtcgag cgccacgcct
     1681 gcccgtcgta gctcccgtcg agcgacgaga tgaagagcgg gccgtcgttc gcgcacccgt
     1741 tcgggaacga gccgcctgcg aactcctcct cggtcgcgta gatgaggttc ccgtaggacg
     1801 ggtcgggggc gtcgccgccg atcggacgcc acgcgttgtg catgaagcgc gacgaggtcc
     1861 gcgtgggcgc gaccgtctcc tcgaagccgc cgcccgcgta cgggacgggg ttccaggcgg
     1921 tcgcgacgcg cgcggcgccg agggtcgggt cgtagtggag ccccttcgtg tggtagccgc
     1981 gcacgccgcc tcggcccgag acccacgcga tgcccatgtc gtccacctgg acgtcgtgcg
     2041 cgtagtcggt ccggccgtcg ttccgggcga ggtcgaccgg ctccggatac acggagggct
     2101 tgtgcgggtt gcgcatgtcc gtgacgaaga tcgggcgccc ggcccagccc atcgcggcct
     2161 ggtcggtgtt cggcgccggg ccgcccgtcc agagccagtt gcagtcgttc acgcacgtcg
     2221 tcgtgtggcc gccgggcgtt tcgacgaacg tgatgacgcg cagggccgcc ggatcgcgga
     2281 cgtccacgac gtagatcccg gagcgtgagg ccgggttgcc ggtgctcccg ccgtacgcgc
     2341 gcgggtcgcg cgagaggaac acgagcttgc ggtcggcgtc cacgtccatg tcctcgttct
     2401 cccacatgtc gtcgagcgtc ccccggcgcg gcgcctgcga gccgttgccc gggttgccgt
     2461 cctcgaggaa cgggtcgccg atcccgtccc cgagccagtc gaggagcagc ggccgtgtcg
     2521 gcaccgtcat gtcgtaggac ttgaggccga accggccgga gacgaacatg acgtcgcgcg
     2581 tcgccgcggt gagcggctgg tcttcgcgcg ggttcccgta gtcgatgaag ttgatcgaga
     2641 tcgtctgcgt gagcgtctcg cggtagccgg agggcgtgct cgggtcggct gcgagcgtgc
     2701 cgtccgcgtt ccacgtgtgg cggacggtgt cgatgcggca gagcacctcg acgttcttca
     2761 ccgaccagtt cgcggcagga ggcacctgac ggcaccagtt gcggtcctga ggcggcgtcg
     2821 gcgtcgggct cgcggccgcc gggccggcga cgcagagcgc gaggacgaag acgaggacgg
     2881 atgtcaggcg gcgcatgggc agtcctttcg ttcggttctc ctgacgggcg cgccgaccgt
     2941 acacccgcgt catgcgcgaa gcaacgaaag gcggcgcgca cgtttctcca cgcagcgccc
     3001 ggccccgcag gcggaaccgg cggggccggg cgtgcgtacg cctctcagct cgcgggatgc
     3061 ggctcgagcc gctcggccgc agcgcgatcg cgtgcctgcc ggtcccaggc gatgtcgagg
     3121 ccgaggagga tcaggttcgc gccggccccg gccccgatga tcgtcgtcag gagggcgacg
     3181 agcccctccg tcgtgttgaa cccgagccac gcaccgacga gggcgccgct cgtcgccgcg
     3241 gcaagcccgg tcgccttgac cgccgccgac cacgcgcgct gcacccaggc gaggtagacg
     3301 ccgaggccga tcggcacgcc gatcgagaag ccggcgagca gctcgtcgtc gagcggcacg
     3361 cccggcatca ccgtgaggac gacgaggacg ccggcgaacc agccgccgag gccgagcacg
     3421 agcgggaaaa ccgaccgcag caccgcagcc gccttccgac cgaacccgcc gcgcctgtga
     3481 acccggcgcg ccatcgcgag cagcgcgccc gccgcgacga cgacgagggc gagcatcagg
     3541 ccgaggagga tcttcgccat cctgacctgg gtgaggggcg gcgagaagtc gacgtccccg
     3601 cgctcgtaga gcgaggcgtc gacccggccg cttgcgaggt acgtgttgac gaggcggctc
     3661 gaggcctccg gctgctcggc ccagaagtcc gccgtatgcc cgagctcgcg cagcacgacc
     3721 tgctcgccgt tcgggaggta cggcatgagc tcggccgtcg ccacctgcgg cggcgtcgag
     3781 ccgtcgagct cgccgctgat cacgagcgtc tcgacctccg acgtccgcat gcggctgtag
     3841 cggttctcct cgtgcgccgc cggccagccg tcggccagcc gcccgcctcc ccagccgtac
     3901 gcgctcgcgg ccgtcccgag gttcgcggcc ggatcaacac cgccggcgaa gtactcgcgc
     3961 gacgccgcgg cgtcgacgcg gccggccgcc gcgtactggc cccagacgaa catcttcggg
     4021 aagatgagct ccccggcgag cgacgcgaac cagaacccgc tcgggtcgcc ctcggccgcc
     4081 gacagccacg agtcgatcgt ctgcgggccg tacgccacgg gcgacgcgga ggtcgactcc
     4141 atgaggccga agaaggaggc gatgcgcacg ctgctctcct ggatcggcag gaacgcccag
     4201 cggtcgggga tgtcctcgtt cgtccgggcg agcgacgcgg cgaggtcgtc cgtccgctcg
     4261 ctgcagctcg cgtcgtcggc gcagagcttc gcgtagcggg cgatctgctc gtccgtcgtc
     4321 tcggcgtccc agaggaagtg gcccggcggg ttcgccgcga cgagcaccga ccggtggatg
     4381 ctctccgggt agcgccaggc gtagatgagc gccgtgcgcg tcccggcgct ctcgctcagg
     4441 aggtcgatcc gctcgtagcc gagggccttg cgggcgtcct ccaggtcgtc cacctgctgc
     4501 acgaggccgt acgacgagac gtcgacaccc tcgtcggcga gccggctcgc gcaggcgcgg
     4561 aacgcgccct cgtacgcgct gaaggacgcg tcaccgaggt agtcggtcga atgggcgagc
     4621 gcggactcga cctccgggca atcgagccgc tcggagccgt ccacgccgcg atagccgacg
     4681 aggacgacgt cgcggtcgcc ggcgaaccgg ctcgccccgg ggaactccat gttggtcccg
     4741 ccgggaccgc cttcgaggta gaagaccggc tcgccggggt cgtccgtttc ggccttgatg
     4801 cgcgtgaccg gcagcgcgat gagtcgcgag tcggggtcgg cgcggttctc cggcacgacg
     4861 agcgtcccgc agtccgccgc gtacgtgccg tcctcggtct cgtagtcgca cggctcgagg
     4921 acgatgtcgc ccgcctgtgc gcccgcgggc accgagaccg ggccgtcctc gggcgcgaag
     4981 cggaggtatc cgagcgcgag gacggcgagg gcgacgaggg ccagcgcgac gatgcgcgcc
     5041 ggcgtgaatg cgcgtgtggt gttgcgttcc atgactgctc cttcggggtg gattccatgc
     5101 cctcgacgct atgagcgggg ccccggaacg gcgtcgtcgg gaggccggat tggcgctcgg
     5161 actcgccgac gattcggcgt cctcccgagg taggagcccg aacgccgtcg gggcccgccc
     5221 ccggtgagga ggcgagcccc gagaggcgga tccccctgcg gctacgagcc ggcaggcgag
     5281 ggagccgcga cgggcggcgc gggagcctcg gtctcgtgcg agaccttcgg cagccactcg
     5341 agccacctcg gcaggtacca gttcctctcg ccgagcagct tcatcgccga cgggaggagc
     5401 acggcgcgga cgatcgtcgc gtcgatcagg atcgccgcgg cgagcccgac gccggcctgc
     5461 ttcatctcga tcgtcgagag cgtcgcgaag atcgagaaca cggcgaccat cacgatcgcc
     5521 gcggccgaga cgacgcccgc ggtcgtcttg atcccgtgcg tcacggccgc gtcggtgtcc
     5581 agcccccggt cgtacgcctc gcgaatccgg ctcaggatga acacgtggta gtccatcgag
     5641 aggccgaaga ggacgacgaa catgaagagc ggaagccacg cggtgatcgc cccggtcgac
     5701 tcgaagccca ggagcgactc gccccagccc cactggaaga ccacgacgag gagcccgtag
     5761 gcggccgcga cggagagcag gttcaggacg accgccttcg ccgcgatcac gaccgagcgg
     5821 aacgcgacca gcaggaggcc gaacgcgagg aggaggacga acgcgaagac gatcggcgcc
     5881 cggtccttca cgacctcgct gaagtcgtgc gacgaggccg cctcgccgcc gacggccacc
     5941 tccgcgccgt ccacggcgcc gagcgtggcg gggatgacgt cctcgcggag cgtcatgagg
     6001 gccgccgtcg acgcgtcgtc ggtgccggtg ccgacgagcg gcaccgagat gagcgccacc
     6061 gtgccctcgt cgttcgcctg cacctggatc ggctcctcga cctgcccggt cgcgagcgcg
     6121 cgctcccgga agtcggcgag cgccgcctcg aacgccggcg aggtgacgtc gtccgcctcg
     6181 acggccacga cggccggcag cgggccgccc gggaacgccg cctgcacccg gtcgtacgtc
     6241 tgcatgatcg ggatgtcgcg cgggaggctc gagatgcccg ggaacgcggt gtgcatcccg
     6301 agcgccggca gggcgaggac gacgagcacg cccgcggacg ccacggccga gacgagcggc
     6361 cgccgcagca cgcggtcgag gatcgcgccc cacacccgcg agtcgccgga gggacggcga
     6421 agccggtgga cgaacgggat cttgcccttc tccacccgg
//
LOCUS       DASTFC010000005         5773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1285506,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000005 DASTFC010000000
VERSION     DASTFC010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285506"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFO88_00110"
     CDS             complement(<1..971)
                     /locus_tag="VFO88_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9543954.1"
                     /translation="MRVVVIGASGNTGTSLLPELAREPAVDSVLGVARRLPELDVPKT
                     EWATADVAADDLVPLLRGADAVVHLAWAIQPSRDLEALRRVNVLGSERVFRAVATAAV
                     PALVYASSVGAYSVGPKDRPVDERWATGGIPSSFYSRHKAEVERLLDRFERERPETRV
                     VRMRPGLIFKREAASGIRRLFAGPFLVNPLVRPGLIPVVADLPRLRFQALHSDDAGAA
                     YRLAVVREDARGAFNLAAEPVLDPQVLGRVLGARPVPVPARLARGLTALSWRAHLQPT
                     PPGWLDLALGVPVMDVSRARHGLGWEPRWDAGDALLDLLGGLRDSAGA"
     gene            complement(1127..1432)
                     /locus_tag="VFO88_00115"
     CDS             complement(1127..1432)
                     /locus_tag="VFO88_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543955.1"
                     /translation="MARIIPFALVAAAAVIATLAVNFLLVGYAQNRDDPVGKLSPRAL
                     ITDTGGRTGPATTSGTTTDGTTTDGTTTEGTTTEEDDDDSSGRGRGRGRGRGGDDDD"
     gene            complement(1444..2589)
                     /locus_tag="VFO88_00120"
     CDS             complement(1444..2589)
                     /locus_tag="VFO88_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9543956.1"
                     /translation="MTPRSVLIVGAGLAGSRCAETLRAEGYDGRVVVVGEEPHAPYER
                     PALSKELLAGARTSAELRAPGFWAERGIELRLGERVEAVDVQRRTAGPGLAWDALVLA
                     TGAWARRIPLPTPDGVHVLRTLADAEALREELRPGRRLAIVGAGFVGVEVASTARSLG
                     CEVSVVDVAGVPLERVLGREVGEVLAARYRAYGVDLRLGTGLAGFVAGADGRVRALEL
                     ADGSELPCDAALVAVGVAPSVELHGDPRGGIETDACGRTALPGVYACGDVAAAWRPSL
                     GARLRVEHWTSAAGQAAAVAHAILGRERPYDDLPYFWSDQFGLRLQHVGHAHEWAAVE
                     LEGDESSFAARYLDRTGRPLAGLLGNRPREVGLLRRELAGAARREAA"
     gene            complement(2586..2795)
                     /locus_tag="VFO88_00125"
     CDS             complement(2586..2795)
                     /locus_tag="VFO88_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET9543957.1"
                     /translation="MTYRIVIDGSLCSGFGTCAELAPDQFEVGPDGIALARVGESDDA
                     AVLDAAAQCPMGAIAVYDEAGEQAA"
     gene            complement(2792..3391)
                     /locus_tag="VFO88_00130"
     CDS             complement(2792..3391)
                     /locus_tag="VFO88_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET9543958.1"
                     /translation="MKSDPTFWILARATGLAAFVLLTVSVLAGLVVKSRPFGTRLKPA
                     TATDLHRTLALLSLSAVLLHVGTLLVDDAANIGVGAVLVPGLSPYRPVATGLGVLAFE
                     LMVLVYASFSLRRRIGQKNWRRLHHTTFGLFALAAGHGLFAGSDTAAPWGLGLYLGAI
                     GAVAAALAWRLLVPPARPQPRPRPAAASAPAPQPKEEPA"
     gene            complement(3388..4251)
                     /locus_tag="VFO88_00135"
     CDS             complement(3388..4251)
                     /locus_tag="VFO88_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET9543959.1"
                     /translation="MERHAFHALGTDCEVLLDVEPGPDAVLALATAEGEIRRLERLLS
                     RFRPDSELSRLNEAGALEAGDDLLAVVRLALEARERTGGRFDPTVLGALVAAGYDRSF
                     DELRPDGAGSLSSPAGGAVRVRGRRIELDRGVRLDLGGIGKGYAVDRAVARLAGVGPS
                     LVNAGGDLAVAGVPEGGVWPVGLDTPLGGITLGVAEGALATSGSDRRRWRAGGEERHH
                     LIDPHTSRPADSDLVTVTVAAPTAVEAEVAAKALFLAGEQAAAAEADALGLPALLVTV
                     DGRVRYAGGLA"
     gene            complement(4251..4601)
                     /locus_tag="VFO88_00140"
     CDS             complement(4251..4601)
                     /locus_tag="VFO88_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543960.1"
                     /translation="MTDHVARLYATALALVVFFVSWAAVAARPWGHEAEAAKPDPRIA
                     QLDRRAEKLRKRQVRVQRQLDRRFEAYRVELARRQALSVAAPVSPAPVAAPAVAAPSV
                     STVPAAPVTQTRSS"
     gene            complement(4594..5004)
                     /locus_tag="VFO88_00145"
     CDS             complement(4594..5004)
                     /locus_tag="VFO88_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543961.1"
                     /translation="MHKAHALIIALALGVAAVAGTFAALQTTSVGAQAAGQSASAAEI
                     AKRQAKLDKAEKKLARAAKRKPPALPPLSAGGAAVAAAPAAGTAFVAAQPGPVAPVAH
                     DDDRDDDHDDDWDDDDRHDDDWDDDHEDDDEDDD"
     gene            5147..>5773
                     /locus_tag="VFO88_00150"
     CDS             5147..>5773
                     /locus_tag="VFO88_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9543962.1"
                     /translation="MKILIVEDEDAIAVPLAEGLSREGFEVTRVANGADALAVPEPDL
                     VLLDLRLPDMDGLDVCRELRARSQVPIIVVTAKGEEVDRVVGLEVGADDYVVKPFGLR
                     ELVARIRAVSRRTGARAEGGADEDGPLAVGPLEIDLRAHRVTLEGDEVALTAKEFDLL
                     TLLAREAGAVVSRERILAEVWNTTWYGSAKTIDVHVASLRRKLGDPSWI"
BASE COUNT          730 a         2213 c         2192 g          638 t
ORIGIN      
        1 gcgccggccg agtcccgcag gccgccgagc aggtcgagga gcgcgtcgcc ggcgtcccac
       61 cgcggttccc accccagccc atgacgcgcc cgcgacacgt ccatgacggg cacgccgagg
      121 gcgaggtcga gccagccggg cggcgtcggc tgcaggtgcg cgcgccagct cagcgccgtg
      181 aggccgcggg cgaggcgcgc cggcaccggc accggacgcg cgccgagcac ccggccgagc
      241 acctgcggat cgagaactgg ctcggcggcg aggttgaacg ccccgcgcgc gtcctcgcgc
      301 acgacggcga gccggtaggc cgcgcccgcg tcgtccgaat ggagcgcctg gaagcgcagc
      361 cgcggcaggt cggcgacgac ggggatgagc cccggccgga cgagcgggtt cacgaggaac
      421 ggccccgcga agagccgccg gatcccgctc gccgcctccc gcttgaagat gagccccggc
      481 cgcatccgga cgacgcgcgt ctccggccgc tcccgctcga agcggtcgag cagccgctcc
      541 acctccgcct tgtggcgcga gtagaagctc gacgggatgc cgccggtcgc ccagcgctcg
      601 tcgaccggac ggtccttcgg cccgaccgag tacgcgccga cggacgacgc gtagacgagc
      661 gccgggacgg ccgccgtggc gacggcccgg aacacgcgct cggagccgag cacgttgacc
      721 cgccgcaggg cctcgaggtc gcgcgagggc tggatcgccc aggcgaggtg gacgaccgcg
      781 tcggcgccgc ggagcagcgg cacgaggtcg tccgcggcga cgtccgccgt cgcccactcc
      841 gtcttcggga cgtcgagctc gggcaggcgc cgagcgacgc cgagcaccga gtccacggcc
      901 ggctcgcggg cgagctcggg aagcaggctc gtgcccgtgt tcccggacgc accgatgacg
      961 acgacgcgca tgtcccgccc gtacccgccg gggccaccgc gaatccggac gcgaaacggg
     1021 cggcggactc cccgtctcgc cgcccgtctc acttgccccc caggaggtga tgccccccgt
     1081 tcgtcggcgc gatgctcccc gccgcggcgc cgcctcaaac gcgttctcag tcgtcgtcgt
     1141 ccccgccgcg gccgcgaccc cgcccgcgcc cgcggccgct cgagtcgtcg tcgtcctcct
     1201 cggtcgtcgt gccctccgtc gtcgtgccgt cggtggtcgt gccgtcagtc gtcgtcccgg
     1261 aggtcgtcgc cggccccgtc cgcccgcccg tatcggtgat gagcgcgcga gggctcagct
     1321 tcccgaccgg gtcgtcgcgg ttctgcgcgt agccgacgag gaggaagttg accgcgagcg
     1381 tcgcgatcac ggcggccgcc gcgacgagcg cgaacgggat gatgcgtgcc atccgcccca
     1441 aggttacgcc gcctccctcc gggccgcccc cgcgagctcg cgccggagga gaccgacctc
     1501 gcgcggccgg ttcccgagca acccggcgag cgggcgcccg gtgcggtcga ggtagcgcgc
     1561 cgcgaaggac gactcgtcgc cctcgagctc gacggccgcc cactcgtgcg cgtggccgac
     1621 gtgctggagc cgcagcccga actggtccga ccagaagtac gggaggtcgt cgtacggccg
     1681 ctcgcggccg aggatcgcgt gcgcgaccgc cgccgcctgg cccgccgcgg acgtccagtg
     1741 ctcgacgcgg agacgcgcgc cgagcgacgg ccgccaggcg gcagcgacgt ccccgcaagc
     1801 gtagacgccg gggagcgcgg tgcgcccgca cgcgtccgtc tcgatcccgc cgcgcgggtc
     1861 gccgtggagc tcgaccgagg gcgcgacgcc cacggcgacg agcgccgcgt cgcacggaag
     1921 ctccgagccg tccgcgagct cgagcgcccg cacccggccg tccgcgcccg cgacgaagcc
     1981 cgcgaggccc gtgccgagcc ggaggtcgac gccgtacgcg cggtagcggg cggcgagcac
     2041 ctcccccacc tcccgcccga gcacgcgctc gagcgggacc ccggcgacgt ccacgacgct
     2101 cacctcgcac ccgagcgagc gcgccgtcga cgccacctcg acgccgacga agcccgcgcc
     2161 gacgatcgcg aggcggcgac ccgggcgaag ctcctccctg agcgcctcgg cgtccgcgag
     2221 cgtgcggagg acgtggacgc cgtccggcgt cggaagcggg atgcgccgcg cccaggcccc
     2281 ggtcgcgagg acgagcgcgt cccacgcgag ccccgggccg gccgtccgcc gctggacgtc
     2341 cacggcctcg acgcgctccc cgagccgcag ctcgatcccg cgctcggccc agaacccggg
     2401 cgcccgcagc tcggcgctcg tgcgggcgcc cgcgaggagc tccttcgaga gcgccggccg
     2461 ctcgtacggt gcgtgcggct cctcgccgac cacgaccacg cggccgtcgt agccctccgc
     2521 gcgaagcgtc tcggcgcagc gggagccggc gaggcccgcg ccgacgatga ggacgctgcg
     2581 cggggtcatg ccgcctgctc cccggcctcg tcgtacacgg cgatcgcccc catcgggcac
     2641 tgggccgccg cgtcgagcac ggccgcgtcg tcggactcgc ccacgcgggc gagggcgatc
     2701 ccgtccggcc cgacctcgaa ctggtcgggg gcgagctcgg cgcaggtgcc gaagccgctg
     2761 cagagcgagc cgtcgatcac gatgcggtac gtcatgcggg ttcctccttc ggttgcggtg
     2821 ccggtgcgga tgcggccgcc gggcggggcc gcggctgcgg cctggccggc gggacgagca
     2881 gccgccacgc gagggcggcg gcgacggcgc cgatcgcgcc gaggtagagc ccgaggcccc
     2941 agggagctgc cgtgtccgag cccgcgaaga gcccgtgccc ggccgcgagc gcgaagagcc
     3001 cgaacgtcgt gtggtgaagc cggcgccagt tcttctgacc gatccgccgg cgcagcgaga
     3061 acgacgcgta gacgagcacc atgagctcga acgcgagcac gccgagcccc gttgcgaccg
     3121 gccggtacgg cgagagcccg gggaccagca ccgcgccgac cccgatgttc gccgcgtcgt
     3181 ccacgagcag cgtcccgacg tggagcagga cggccgagag cgagaggagc gcgagcgtcc
     3241 ggtgcaggtc ggtcgcggtc gccggcttca ggcgcgtccc gaacggccgc gacttcacga
     3301 cgagcccggc gagcacggac acggtcagga ggacgaacgc ggcgagcccg gtcgcgcgcg
     3361 cgaggatcca gaaggtcggg tcgctcttca cgcgagccct cccgcgtacc ggacgcgtcc
     3421 gtccaccgtc acgaggagcg ccgggagccc gagcgcgtcg gcctccgcgg cggcggcctg
     3481 ctcgcctgcg aggaagagcg ccttcgccgc gacctcggcc tcgaccgccg tcggcgcggc
     3541 gaccgtgacc gtcacgaggt cggagtcggc cgggcggctc gtgtgcggat cgatcaggtg
     3601 gtgacgctcc tcgcctcccg cgcgccagcg gcgccggtcg gagccggacg tggcgagcgc
     3661 tccctcggcg acgccgagcg tgatcccgcc gagcggcgtg tcgagcccga ccggccagac
     3721 gccgccctcc gggacgcccg cgacggcgag gtcgccgccc gcgttcacga gggagggccc
     3781 gaccccggcg aggcgggcga ccgcgcggtc gaccgcgtac cccttgccga tcccgccgag
     3841 gtcgagccgg acgccccggt cgagctcgat ccggcggcct cgcacccgga cggcgccgcc
     3901 cgcaggagag gagagcgagc cggcgccgtc cgggcggagc tcgtcgaacg agcggtcgta
     3961 cccggccgcg acgagcgcgc ccagcacggt cgggtcgaac cgcccgcccg tccgctcgcg
     4021 cgcctcgagc gcgagccgga cgacggcgag gaggtcgtcc ccggcctcga gcgcgcccgc
     4081 ctcgttcagg cgcgagagct cggagtccgg ccggaagcgg gagaggagcc gctccagccg
     4141 ccggatctcg ccctcggcgg tcgcgagcgc gagcaccgcg tcggggccgg gctcgacgtc
     4201 gagcaggacc tcgcagtcgg tgccgagcgc gtgaaaggcg tggcgctcca tcacgagctc
     4261 cgcgtctgcg tcaccggcgc ggccggcacg gtcgagacgg acggagccgc gacggccggc
     4321 gccgcgaccg gggcggggct caccggggcg gcgacgctca gggcctgccg gcgtgccagc
     4381 tcgacgcggt aggcctcgaa gcgccggtcg agctgccgct ggacgcggac ctgccgcttc
     4441 cggagcttct ccgcgcgccg gtcgagctgc gcgatgcgcg ggtccggctt cgcggcctcg
     4501 gcctcgtgcc cccagggccg ggcggcgacg gccgcccacg agacgaagaa gacgacgagc
     4561 gcgagcgccg tcgcgtacag ccgggcgacg tgatcagtca tcgtcctcgt cgtcgtcctc
     4621 gtggtcgtcg tcccagtcgt cgtcgtggcg gtcgtcgtcg tcccagtcgt cgtcgtggtc
     4681 gtcgtcccgg tcgtcgtcgt gcgcgaccgg agctaccggg cccggctgcg ccgcaacgaa
     4741 ggcggtgccg gcagccggcg cggcggcaac ggcagccccg ccggccgaga gcggcggcag
     4801 ggccggcggc ttgcgcttcg ccgcgcgggc gagcttcttc tcggccttgt cgagcttcgc
     4861 ctggcgcttg gcgatctccg ccgccgaggc cgactgtccc gcggcctggg cgccgacgga
     4921 ggtcgtctgc agcgccgcga acgtcccggc gacggcggcg acgccgagcg cgagggcgat
     4981 gatgagtgcg tgagccttgt gcatggagcc tccttgacgg gtgcggtcgc accgtaggaa
     5041 ggcccccgca agatccgggc tcggcgaagg cgcacgttcg gttcagccga ggtaaagctt
     5101 cggaaggcgg cgggcgcgcg gcctgcccgc atgccaccat gcgcccgtga agatcctgat
     5161 cgtcgaggac gaggacgcga tcgccgtccc gctcgccgag ggcctctccc gcgagggctt
     5221 cgaggtgacg cgcgtggcga acggcgccga cgcgctggcc gtcccggagc ccgatctcgt
     5281 gctcctcgac ctgcggttgc cggacatgga cggccttgac gtctgccgcg agctgcgcgc
     5341 ccgctcgcag gtgccgatca tcgtcgtgac cgcgaagggc gaggaggtcg accgcgtcgt
     5401 ggggctcgag gtcggggcgg acgactacgt cgtgaagccg ttcggcctcc gcgagctcgt
     5461 cgcgcggatc cgggcggtct cgcgccgcac cggcgcgcgg gccgagggcg gcgccgacga
     5521 ggacggcccg ctggcggtcg gcccgctcga gatcgacctc cgcgcgcacc gggtgacgct
     5581 cgagggcgac gaggtcgcgc tcacggcgaa ggagttcgac ctcctcacgc ttctcgcgcg
     5641 cgaggcgggc gcggtcgtga gccgcgagcg gatcctcgcc gaggtctgga acacgacctg
     5701 gtacggatcg gcgaagacga tcgacgtcca cgtcgcgtcg cttcgccgca agctcggcga
     5761 cccgagctgg atc
//
LOCUS       DASTFC010000006         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_609845,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000006 DASTFC010000000
VERSION     DASTFC010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609845"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFO88_00155"
     CDS             <1..600
                     /locus_tag="VFO88_00155"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9543963.1"
                     /translation="QDAVPKKAHYRKFGIRRADGQDDFAMMAEVVSRRFARAGVSVAD
                     DEYDEGFAAVPNLVVVDGGKGQLSAALAAMQAYDLPRVAVIALAKREEEVFLPGDPEP
                     VRLDASSPALQLLQRIRDEAHRFALGFHRQRRGARAHESILDALPGVGPARKRALLRH
                     FGSPERLLAAAPEDFEGVPGVPAKTGRAIYEALHKAGGA"
     gene            complement(702..2033)
                     /locus_tag="VFO88_00160"
     CDS             complement(702..2033)
                     /locus_tag="VFO88_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543964.1"
                     /translation="MTPLAHGVGSVQDLPVPQWLFLYGAGVVLVVSFAALALLWRQPL
                     LARHANGRPLPAGLSRVVLSPAVRIALGALAVFLLFVVFAAALVGERSVGSNLAPTFV
                     YVAFWLGLVPLTVVLGNIWRALNPWRAAAEGAEWLAGRLGAQAEPYPYPERLGRWPAA
                     LGLFLFAALELAYFEPADPRALALAIVLYSGATWIGALVFGSRAWFPNAEAFTVYFGL
                     LARIAPFAVRDGRVVVRWPTVGLAGGSTPRPGTVAFVAVMLGSVAFDGFSRSSFWVDE
                     TANLGDLTGSLVRLAGLLVWIAAVFGIYHLTVFAAKRLASTDLDLAGAFVWSLVPIAL
                     VYAVSHYFSLLVIEGQFLVTLASDPFGFGWDLFGTRDFRPNLAPFAPSTIWYVQVGTL
                     VAGHVVGLAVAHDRALELFPRDTGRALRTQYPFLVLMVIYTVAGLYLLSQE"
     gene            complement(2030..2509)
                     /locus_tag="VFO88_00165"
     CDS             complement(2030..2509)
                     /locus_tag="VFO88_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543965.1"
                     /translation="MGNAGRIGIGVAALVVAVVLFIVLRPGDEDASPASETTAAETTT
                     PATTTTEATETETGETGTEAEETTTEEGPAQFRVDVPASGPQGVQRLNARQGDEVELV
                     VTLGYHDDVHLHGYDLLIHTGPGLPPARFEFEATTPGRFEIETETAHQLIAELRVRP"
BASE COUNT          404 a          964 c         1029 g          307 t
ORIGIN      
        1 caggacgccg tgccgaagaa ggcgcactac cgcaagttcg ggatccgccg cgcggacggg
       61 caggacgact tcgcgatgat ggccgaggtc gtgtcccggc ggttcgcccg cgccggtgtc
      121 tcggtcgccg acgacgagta cgacgagggc ttcgccgccg tcccgaacct cgtggtcgtg
      181 gacggcggga agggccagct ctccgcggcc ctcgcggcga tgcaggccta cgacctgccg
      241 cgcgtcgcgg tgatcgcgct cgccaagcgc gaggaggagg tcttcctgcc cggcgacccg
      301 gagccggtgc ggctcgacgc ctcctcgccg gcgctccagc tcctccagcg gatccgcgac
      361 gaggcgcacc gcttcgcgct cggcttccac cgccagcggc gcggcgcgcg ggcgcacgag
      421 tcgatcctcg acgcgctccc cggggtcggg ccggcgcgca agcgggcgct cctgcgccac
      481 ttcggttcgc cggagcgcct cctcgccgcg gcaccagagg acttcgaggg cgtccccggc
      541 gtcccggcga agacgggccg cgcgatctac gaggcgctgc acaaggccgg cggcgcctag
      601 tccgttcggg ccgcgccccc ggctgccgtg tccggtgcca ggcacgggac agtggccgcg
      661 gcgggcgtgc ccggacgcaa tcgtctccca gcggccgtgc gctactcctg ggagagcaga
      721 taaagcccgg cgaccgtgta gatcaccatg aggacgagga acgggtactg cgtgcggagc
      781 gcccgtccgg tgtcgcgcgg gaagagctcg agcgcacggt cgtgggcgac cgcgaggccg
      841 acgacgtggc ccgcgacgag cgtcccgacc tggacgtacc agatcgtcga cggcgcgaac
      901 ggcgcgaggt tcggccggaa gtcgcgcgtc ccgaaaaggt cccagccgaa cccgaacggg
      961 tcggacgcga gcgtgacgag gaactggccc tcgatcacga gcagcgagaa gtagtgcgag
     1021 accgcgtaga cgagcgcgat cggcacgagg ctccagacga acgcgcccgc gaggtcgagg
     1081 tcggtcgacg cgaggcgctt cgccgcgaag accgtcaggt ggtagatacc gaagacggcg
     1141 gcgatccaga cgaggagccc ggccaggcgc acgagcgagc cggtcaggtc gccgaggttc
     1201 gcggtctcgt cgacccagaa ggacgagcgg ctgaacccgt cgaaggcgac cgacccgagc
     1261 atgacggcga cgaacgcgac ggtgccgggg cggggggtgg agccgcccgc gagcccgacc
     1321 gtcggccagc ggacgacgac gcgcccgtcc cgcaccgcga acggcgcgat ccgcgcgagc
     1381 agcccgaagt agacggtgaa ggcttccgcg ttcgggaacc aggcgcggct cccgaagacg
     1441 agcgccccga tccacgtcgc gccgctgtag agcacgatcg cgagcgcgag cgcccgcggg
     1501 tcggcgggct cgaagtaggc gagctcgagc gcggcgaaga ggaacaggcc gagcgcggcg
     1561 ggccagcggc cgagccgctc cgggtacggg tacggctccg cctgggcgcc gagccgcccc
     1621 gcgagccact cggcgccctc ggcggcggcg cgccaggggt tgagcgcccg ccagatgttc
     1681 ccgaggacga cggtcagcgg gacgagcccg agccagaacg cgacgtagac gaacgtcggc
     1741 gcgaggttcg acccgaccga ccgctcgccg acgagcgcgg ccgcgaagac cacgaagaga
     1801 aggaagacgg cgagcgcgcc cagcgcgatc cgcacggcgg gagagaggac gacccggctg
     1861 agaccggcgg gaaggggccg cccgttcgcg tggcgcgcga ggagcggctg ccgccagagg
     1921 agcgcgagcg ccgcgaacga gacgacgagg acgacccccg ctccgtagag gaacagccac
     1981 tgaggaaccg gcaggtcctg gacgctgccg acgccgtggg cgagcggcgt cacgggcgga
     2041 cgcgcagctc cgcgatcagc tggtgggccg tctccgtctc gatctcgaag cggcccgggg
     2101 tcgtcgcctc gaactcgaag cgcgcgggcg gcagccctgg cccggtgtgg atcaggaggt
     2161 cgtacccgtg gaggtgcacg tcgtcgtggt acccgagcgt cacgacgagc tcgacctcgt
     2221 cgccctgccg cgcgttcagc cgctgcaccc cctgcggccc gctcgcgggg acgtcgacgc
     2281 gaaactgcgc cgggccctcc tcggtcgtcg tctcctcggc ctcggtcccg gtctcgccgg
     2341 tctccgtctc cgtcgcctcg gtcgtggtcg tggccggcgt ggtcgtctcc gccgcggtcg
     2401 tctcggaggc cggcgaggcg tcctcgtcgc cggggcggag aacgatgaag agcacgacgg
     2461 ctacgacgag cgcagcgacg ccgatcccga tgcgcccggc gtttcccacg cggccattat
     2521 gtccgcggcc tctcgccgcc cggccgggga accggtgccg ccggggccgc tagccctggc
     2581 tcccgggctc gtccggctcg aggaacgggt agcggtagtc gcgcggcggg gcgaacgtct
     2641 ccttgatcgt ccgcgggctc acccagcgga tgaggttcca catcgagccc gccttgtcgt
     2701 tcgt
//
LOCUS       DASTFC010000007         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1433891,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000007 DASTFC010000000
VERSION     DASTFC010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433891"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFO88_00170"
     CDS             <1..510
                     /locus_tag="VFO88_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9543966.1"
                     /translation="LAEDLLVLAATEDGDLPVRGAPVDARELMEEVADRFAARAQAAG
                     RELRVRAARSVLHADRARLQQALGNLVDNALRHGAGRIVLDATAVGGCTVLSVSDEGP
                     GFPPGFEGRAFERFSRGDEARSSGGAGLGLAIVDAVARAHGGEARARTRPEGGAEVAL
                     FLPSRLSLR"
     gene            complement(497..763)
                     /locus_tag="VFO88_00175"
     CDS             complement(497..763)
                     /locus_tag="VFO88_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9543967.1"
                     /translation="MSVEVRLPATLPPLFPGLPRRLELEAATVGEALDELDERWPGIR
                     DRLCEPGPVLRRHIHVYVNGERGAVETPLPAEARVDVIAAISGG"
     gene            complement(760..1830)
                     /locus_tag="VFO88_00180"
     CDS             complement(760..1830)
                     /locus_tag="VFO88_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543968.1"
                     /translation="MKTIVLVGTRKGCFVLEREGDGGWTTRGPYCEGWPVFHAIWDAE
                     TGTIFAAAASQWHGASVWRSSDLGETWAQSSEGLAYEEDGLKLSKISGLTAAHGRVLA
                     GAENPGVFASEDGGETWSLVTRLEGQPGSDKWNDPANQPPGNLGVPAILPHPTDPAHW
                     WAIVQGVGIFETTDGGGSFTPRNEGLRADWPLEDPRVGYCVHKLVMSPVDETRLYQQN
                     HVGMHRSDDGGQSWTEITEGLPTEFGFPAAAHPHDRDTLYVIPVDPGHGRTMPEGRAA
                     VWRTRDAGSTWERHDEGLPQRDAYLGVLREGLAIDTRDTPGLYFGTSTGQVFASEDEG
                     ESWAEIASYLPGIRSVEVGLVG"
     gene            complement(1912..2184)
                     /locus_tag="VFO88_00185"
     CDS             complement(1912..2184)
                     /locus_tag="VFO88_00185"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET9543969.1"
                     /translation="MCRNIRTLYNFEPPANDEEVRAAALQYVRKISGFNKPSQANAAA
                     FERAVEDVAAISARLLGELVTNAPPKDREVEAAKRKARAAERYAAA"
     gene            2248..>2483
                     /locus_tag="VFO88_00190"
     CDS             2248..>2483
                     /locus_tag="VFO88_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9543970.1"
                     /translation="MPAATPTRRPELEAEFELHRTELTAYCYRMLGSADAEDAVQETF
                     IRAWRAYDRFEGRAQVRSWLYKIATNVCLDQLESR"
BASE COUNT          300 a          940 c          888 g          355 t
ORIGIN      
        1 ctcgccgaag acctcctcgt cctcgccgcg acggaggacg gcgaccttcc ggtccgcggg
       61 gccccggtcg acgcgcggga gctcatggag gaggtcgcgg accgcttcgc cgcgcgtgcg
      121 caggcggccg ggcgggagct ccgcgtgcgt gcggcccggt ccgtgctgca cgccgaccgc
      181 gcgcgcctcc agcaggcgct cgggaacctg gtcgacaacg cgctgcggca cggggccggc
      241 cgtatcgtgc tggacgcgac ggcggtcggc ggctgcaccg tgctcagcgt gagcgacgag
      301 ggcccgggct tcccacccgg cttcgagggc cgcgcgttcg agcgcttcag ccgcggcgac
      361 gaggcgcgct cgtcgggagg agcaggcctc ggcctcgcga tcgtggacgc cgtcgcgcgg
      421 gcgcacggcg gtgaggcgcg ggcacgcacc cgcccggaag gcggggcgga ggtagctctc
      481 ttcctcccct cgaggctcag cctccgctga tcgcggcgat cacgtccacg cgcgcctccg
      541 cggggagcgg cgtctcgacc gcgccgcgct cgccgttcac gtacacgtgg atgtggcgcc
      601 ggagcacggg gccgggctcg cacaggcggt cgcggattcc cggccagcgc tcgtcgagct
      661 cgtcgagcgc ctcgccgacc gtcgccgcct cgagctcgag ccgccgcggg agcccgggga
      721 agagcggcgg cagcgtcgcc ggcaggcgga cctcgacgct catccgacca gcccgacctc
      781 gaccgacctg atcccgggga ggtagctcgc gatctccgcc cagctctcgc cctcgtcctc
      841 gcttgcgaac acctgcccgg tgctcgtccc gaagtagagg cccggcgtgt cgcgcgtgtc
      901 gatcgccagc ccctcgcgca ggacgcccag gtacgcgtcg cgctgcggca ggccctcgtc
      961 gtgccgctcc cacgtcgagc ccgcgtcgcg cgtgcgccag acggccgcgc gcccctccgg
     1021 catcgtccgc ccgtggcccg gatcgaccgg gatgacgtag agcgtgtccc ggtcgtgcgg
     1081 atgcgccgcg gcggggaagc cgaactccgt cggcaggccc tcggtgatct ccgtccagga
     1141 ctgcccgccg tcgtccgacc ggtgcatgcc gacgtggttc tgctggtaga ggcgcgtctc
     1201 gtccacgggg gacatgacga gcttgtgcac gcagtagccg acccgcgggt cctcgagcgg
     1261 ccagtcggcg cgcaggccct cgttgcgcgg ggtgaacgag ccgccgccgt ccgtcgtctc
     1321 gaagatcccg acgccctgga cgatcgccca ccagtgcgcc gggtcggtcg ggtgcgggag
     1381 gatcgcgggg acgccgaggt tcccgggcgg ctggttcgcg gggtcgttcc acttgtcgct
     1441 ccccggctgg ccctcgaggc gggtgacgag cgaccacgtc tcgccgccgt cctcgctcgc
     1501 gaagacgccg gggttctcgg cgccggccaa gacgcgcccg tgcgcggccg tgagcccgga
     1561 gatcttcgag agcttgagcc cgtcctcctc gtaggcgagc ccctcgctcg actgcgccca
     1621 cgtctcgccg aggtcggagc ttcgccagac gctcgcgccg tgccactggc tcgccgcggc
     1681 ggcgaagatc gtgcccgtct cggcgtccca gatcgcgtgg aagaccggcc acccctcgca
     1741 gtacgggccg cgcgtcgtcc agccgccgtc gccctcccgc tcgagcacga agcacccctt
     1801 ccgggtgccg accaacacga tcgtcttcat cgagccacct ccgcgcgccg tcgtttcagg
     1861 gagtctccac cctgtagacg aacggcgcgc cgaaaaatga tcgccggggc gctacgcggc
     1921 cgcgtaccgc tcggcggccc gcgccttccg cttggccgcc tcgacctcgc ggtccttcgg
     1981 cggcgcgttc gtgacgagct cgccgagcag ccgcgcggag atcgcggcga cgtcctccac
     2041 cgcccgctcg aacgccgcgg cgttcgcctg ggacggcttg ttgaagccgc tgatcttccg
     2101 cacgtactgg agcgcggccg cccggacttc ctcgtcgttc gccggcggct cgaagttgta
     2161 gagcgttctg atgttcctgc acatgcgcgc gatggtagcg agcgatgagt tgcgggcgct
     2221 tctccggtcc tgactaggat gaacgccgtg ccggctgcga cccccacgcg gcgccccgag
     2281 ctcgaggccg agttcgagct gcaccgaacc gagctgaccg cgtactgcta ccggatgctc
     2341 ggctccgccg acgcggagga cgcggtgcag gagacgttca tccgcgcctg gcgcgcctac
     2401 gaccggttcg agggacgcgc gcaggtccgc tcgtggctct acaagatcgc gacgaacgtc
     2461 tgcctcgacc agctcgagag ccg
//
LOCUS       DASTFC010000008         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1615160,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000008 DASTFC010000000
VERSION     DASTFC010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615160"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFO88_00195"
     CDS             <1..400
                     /locus_tag="VFO88_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9543971.1"
                     /translation="LSPADRILAAADVFAALVADRPHRPALGPDDAARTLEAEAGRGL
                     DADAVACVLAAAGRRQAPPPRRRPADLTDREVEVLRLIARGASNRVVAERLVISPKTV
                     GRHVENLYRKIGVSSRAAAAVFAMEQRLLD"
     gene            500..1402
                     /locus_tag="VFO88_00200"
     CDS             500..1402
                     /locus_tag="VFO88_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9543972.1"
                     /translation="MSAVARTDALVAAPPAVDRVALGAFAGVVVIGGTNFVGVRFSNR
                     GLDPLWGAGLRFALAAAIFGLLVAALRLPLPRGRALVLSGAYGLLSFAGAYGCIYWAM
                     QEVPAGVAAVVLALGPLLTLLLAVAHGMERLSGRALAGALVALAGSVVIFFQPDSFAF
                     GWESLALLGVGALFAAQAVVVSKLVGPHHPAAINFVGMSAGAVVLVGAAAVVGERLAL
                     PAGGGTQLALVYLVAATVALFLLALVVVQRWAASAASYAFVLMPVIAVALGAVLAGEP
                     ITPTTVTGGAIVCAGVVVGAARRG"
     gene            1426..2214
                     /locus_tag="VFO88_00205"
     CDS             1426..2214
                     /locus_tag="VFO88_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9543973.1"
                     /translation="MPRIRVNGVSLHYEEQGDGVPILCIHGAGSSGAVWASAARELGR
                     HGRAIVYDRRGSHRSERPEPYETDLRQQAEDAAALLEALDAAPAIVIGRSYGGEVAIE
                     LALRRPESVRALAVLEAGESLTDGGRRWLGELGERVEAAAAEDVGTVGELVLREVLGD
                     EGWEQAPALLKDLFVGNGPAIAAEFRGGFLDVPLEELRRIDCPVLLVGAEDSPPVFAE
                     AIELLAGTLPSARVARVGGGHLIDPADPSVLAFVDEVAAQAASR"
     gene            complement(2196..>2818)
                     /locus_tag="VFO88_00210"
     CDS             complement(2196..>2818)
                     /locus_tag="VFO88_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9543974.1"
                     /translation="EADQEPWCPELDGDVRVSSLQTGLFAGPVGSAVGQHRFHPDAVV
                     REAQENVRLYTPRYGRVELRARFSDDPATMAALWLIGYEDEPERSAELCVCEIFGRDV
                     SPGRALVGMGVHPFGDPRIRDEFERVPVAIDVREPHEYAAEWRPGRTELFVDGESVKV
                     VEQAPDYPLQLMLGLYEFPQPGAPRVPDAYPKAFAVEAVRGYRLAA"
BASE COUNT          294 a         1048 c         1067 g          409 t
ORIGIN      
        1 gctctcgccg gccgaccgga tcctcgcggc cgccgacgtg ttcgcggcgc tcgtcgccga
       61 ccggccgcac cgtccggcgc tcgggcccga cgacgcggcc cggacgctcg aggcggaggc
      121 cgggcgtggc ctcgacgcgg acgccgtcgc ctgcgtgctc gcggcggccg ggcgcagaca
      181 ggccccgccg ccgcgtcgcc ggcctgccga cctcaccgac cgcgaggtcg aggtgctgcg
      241 cctgatcgcg cgcggcgcct cgaaccgcgt ggtggccgag cgcctcgtca tctcgccgaa
      301 gacggtcggg cgccacgtcg agaacctcta ccggaagatc ggcgtctcct cgcgcgccgc
      361 cgccgcggtc ttcgccatgg agcagcggct cctcgactga ccccgcaaag atggggcgtt
      421 cgcccgatgc gggcgccgcc gccggcttcg atactcggcg catggcttcc agcgagcgtg
      481 gcacctcgga gacccggacg tgagcgcggt cgcgcgcacc gacgccctcg tcgcggcgcc
      541 gcccgcggtc gaccgggttg ccctcggcgc ctttgccggg gtggtcgtca tcggtggcac
      601 gaacttcgtc ggcgtccggt tcagcaaccg ggggctcgac ccgctctggg gagccggcct
      661 gcgcttcgcg ctcgccgcgg cgatcttcgg gctgctggtc gcggcgctgc ggctcccgct
      721 tccgcgcggc cgcgcgctcg tgctctccgg cgcctacggc ctgctctcct tcgccggcgc
      781 gtacggctgc atctactggg cgatgcagga ggtgccggcc ggcgtggctg ccgtcgtgct
      841 ggcgctcggg ccgctcctga ccctgcttct cgccgtcgca cacgggatgg aacggctgag
      901 cgggcgcgcg ctcgccgggg cgctggtcgc cctcgccggt tcggtcgtga tcttctttca
      961 gcccgactcg ttcgcgttcg gctgggagtc gctcgccctg ctcggcgtcg gggcgctctt
     1021 cgccgcacag gccgtcgtcg tctccaagct cgtcggaccg caccatcccg cggcgatcaa
     1081 cttcgtcggc atgagcgccg gggccgtcgt cctcgtcggc gccgcggccg tggtcggcga
     1141 acggctcgcg cttccggccg gcggcgggac gcagctcgcc ctcgtgtatc tcgtcgccgc
     1201 cacggtcgcc ctgttcctgc tcgcgctcgt cgtcgtgcag cggtgggccg cgtcggccgc
     1261 gtcctacgcc ttcgtgctca tgcccgtgat cgcggtcgcg ctcggcgccg tgctcgccgg
     1321 cgagccgatc acgccgacga ccgtcacggg cggggcgatc gtctgcgccg gggtggtcgt
     1381 cggcgccgct cggcggggct gaggtcgggg cgggatactg ctcgcatgcc tcgcatccgc
     1441 gtcaacggcg tctcgctcca ctacgaggag cagggcgacg gcgtgccgat cctctgcatt
     1501 cacggcgccg gcagctcggg ggcggtgtgg gcgagcgcgg cgcgtgagct cgggcggcac
     1561 ggcagagcga tcgtctacga ccggcgcggc tcccaccgga gcgagcggcc ggagccgtac
     1621 gagaccgacc tccgtcagca ggccgaggac gccgcggcgc tcctggaggc gctcgacgcg
     1681 gcgccggcga tcgtgatcgg gcggagctac ggcggcgagg tcgcgatcga gctcgcgctt
     1741 cgccgtccgg agagcgtccg cgcgcttgca gtgctcgagg cgggcgagtc cctcacggac
     1801 ggcgggcggc ggtggctcgg ggagctcggc gagcgcgtcg aggccgcggc ggcggaggac
     1861 gtcggcaccg tgggggagct cgtgctccgg gaggtgctcg gcgacgaggg gtgggaacag
     1921 gcgccggcgc tgctcaagga tctcttcgtc gggaacggtc ccgcgatcgc ggccgagttc
     1981 cgcggcggct tcctcgacgt gccgctcgag gagctgcggc ggatcgactg tcccgtgctg
     2041 ctcgtggggg ccgaggactc tccgccggtc ttcgcggagg cgatcgagct cctcgccggc
     2101 acgctcccct cggcgcgggt cgcccgggtc gggggcgggc acctgatcga tccggccgac
     2161 ccctccgtgc tcgcgttcgt ggacgaggtc gccgctcagg ccgcgagccg gtagccgcgc
     2221 accgcttcga cggcgaaggc cttcgggtac gcgtccggca cccgcggtgc gcccggctgc
     2281 gggaactcgt acagcccgag catgagctgg agcgggtagt ccggcgcctg ctctacgacc
     2341 ttcacggact cgccgtcgac gaagagctcg gtgcgcccgg gccgccactc ggcggcgtac
     2401 tcgtgcggct cacggacgtc gatcgcgacc ggcacgcgct cgaactcgtc gcggatccgc
     2461 ggatcgccga acggatgcac gcccatcccg acgagcgcgc ggccgggcga gacgtcgcgc
     2521 ccgaagatct cgcacacgca gagctcagcg gagcgctcgg gctcgtcctc gtagccgatg
     2581 agccagagcg ccgccatcgt cgccgggtcg tcggagaacc gtgcgcggag ctcgacgcgg
     2641 ccgtagcgcg gcgtgtagag cctgacgttc tcctgcgcct cgcgcacgac cgcgtccggg
     2701 tggaagcggt gctggccgac cgcgctcccc accggcccgg cgaagaggcc cgtctggagc
     2761 gaggacacgc ggacgtcgcc gtcgagctcc gggcaccacg gctcctggtc ggcctcga
//
LOCUS       DASTFC010000009         6505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_280276,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000009 DASTFC010000000
VERSION     DASTFC010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280276"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VFO88_00215"
     CDS             complement(<1..1632)
                     /locus_tag="VFO88_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9543975.1"
                     /translation="MDRHAPRAPRLPDPDVSVETLPGVGPKVAQRLARLGLRTVRDVA
                     DHLPRAFLDWSEAGGFDQVLAGTEATVRCTIERIGVRPTRRRNLKLVQASVRDDNGSR
                     VTAVWFNQQWLVDQLPPGTRVLLHGRLEGGFGPPTFRVERHERDTEDAGLHTLGLVPV
                     YPASEDVSTAKLREVTEAALRIARHWPDALPAGVRDAEGLPLRADALLLAHRPERVED
                     AERARERLAFEELLILLVGLVRRRRGAEEGARAHALGAQDGALVAALRASLPFELTPE
                     QEGAIDAIRADLAGEAPMQRLLQGDVGSGKTVVALYALLRAVEAGHQGALMAPTETLA
                     EQHFLTVEPLAREVGVAAVLLTSSLGARALGEARDRVGSGEAQLVVGTHALIQEAVAF
                     RSLAVAVVDEQHRFGVEQRRAISGPAGGRAPHVLHMTATPIPRTLAQVVYGDLAVTEI
                     ARPPRNRKPVVTRWVTHERSAEAYRRLRRHLEEGRQAYVVCPLVSESEAVEARAASEE
                     AERLRHAELSGFRIGCLHGQLPAAERRETMDAFKRR"
     gene            1818..2393
                     /locus_tag="VFO88_00220"
     CDS             1818..2393
                     /locus_tag="VFO88_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET9543976.1"
                     /translation="MKRIFAFGLLVGALALVGVACGGDDDAATDEPAAAMEETTTMEE
                     ESTETTETTSQEMDIVETAVAAGSFDTLVSLVQEAGLAEDLSGPGPLTVFAPTDEAFA
                     AVPEETLTQLQNDPEALRRVLLYHVVEGDVTSDQVTGLDAAATLAGPDLSFSTNGGSV
                     LVNDATVTMADVLASNGVIHVVDAVLLPPAR"
     gene            complement(2455..3318)
                     /locus_tag="VFO88_00225"
     CDS             complement(2455..3318)
                     /locus_tag="VFO88_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET9543977.1"
                     /translation="MRLSSETTAVVLDSTADLPDPAARHPNWRLVPLYVRFGDETYRD
                     HVDLGAVEFYEKLRASDAQPQSSQPTPADFEAAFAELEGFERILCVLISGKLSGTAES
                     ARLAAQALGDDRVTVIDSESVSGGVVILADAVQRRLDRGTTLEEIEEVVARFQRESTI
                     LFTVDTLDYLVRGGRVGKAAGLAGQLLSVKPILAIDEGEVEPLKRVRGRQKALAELLR
                     LFEEGTEDGPALHVGVAHADAEAELETLVGRIRELRPQASLDFQAQLGPVIGTHGGPG
                     TIGLFWFRDEE"
     gene            complement(3302..3694)
                     /locus_tag="VFO88_00230"
     CDS             complement(3302..3694)
                     /locus_tag="VFO88_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9543978.1"
                     /translation="MENAPSTTVLLVEDNEVFREALELMLGVVEDVRVVGAVGDGRAA
                     VDACALLRPDVVLMDYRLPELDGVEATAAIRACSESAVVALTATADDAELDALYDAGA
                     VACLTKDSSLDDIVAAIRSASSRGASLV"
     gene            complement(3757..5346)
                     /locus_tag="VFO88_00235"
     CDS             complement(3757..5346)
                     /locus_tag="VFO88_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET9543979.1"
                     /translation="MPGGALTVVEAALAALEASRRRIDDLNVYPVPDGDTGTNMTMTV
                     RAVRDALAGATELDAEAELALVARSCLMGARGNSGVILSQLVRGAVEGLNGGPETAGD
                     LAPRLRGASDAGYAAVREPQEGTMLTVARELAERAEALDPALPLGDALAEVVAHGEVA
                     LARTQEQLDVLRDAGVVDAGGAGVLELVRGIAAHVRGEPLPEPEVGTGPLPEEAVHRE
                     LSRYRYCTSFFVEGDDAEPDALERELGEFGDSLLVVGARGAVKVHVHTDEPGRAIALA
                     TSVGVIEEVSIENMHVQTAVREERITHADPPPRTAGPTATAVVAVVAGGGNARLFRDL
                     GAAAIVEGGQTMNPPTSQLLQAIDDLDAAGVVLLPNNKNVILAAEQAAAEAGKPVEVV
                     ETTSMQAGLAALVPFDATAALDENAREMREAATAVRSGAVARASRAATIDGIEVAEGE
                     FLGLADGAAVAAGASVEEVARAVAAHLLEPGADVLTILLGDEAPSVNGLRDELAEAHP
                     ALEIDVHEGGQPHYPLLFAAE"
     gene            complement(5372..5563)
                     /gene="rpmB"
                     /locus_tag="VFO88_00240"
     CDS             complement(5372..5563)
                     /gene="rpmB"
                     /locus_tag="VFO88_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET9543980.1"
                     /translation="MSKVCAVCGKSPGFGHNRSHSMVATKRRFNPNLQRVRILVNGVA
                     RRSYVCTRCLKGGKVQKAV"
     gene            complement(5598..6281)
                     /gene="ribH"
                     /locus_tag="VFO88_00245"
     CDS             complement(5598..6281)
                     /gene="ribH"
                     /locus_tag="VFO88_00245"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET9543981.1"
                     /translation="MTERPPGRLEGADELRVTPPFDAEAETRVAPDEREVEDPPAAPA
                     ASAPPAEHAPGRLVIPDGVGAFVGMPNGVRRSVGIVVSRYNGDITTKLLEGALGALTA
                     AGVQRDRIDVLQVPGAFELPLGAMALAKSRRYACVVALGCVIRGETPHFDYIAAEAAS
                     GLQLAALETGVPVVLGVLTTEDRAQAEARAGGAHGNKGEEAASSALEMADVFARLRAV
                     TAAPGPSAA"
     gene            complement(6278..>6505)
                     /locus_tag="VFO88_00250"
     CDS             complement(6278..>6505)
                     /locus_tag="VFO88_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET9543982.1"
                     /translation="IGSQILADLGLTTIRILTNNPRKITGIEGFGLRVTEQVPIEIEP
                     NAENERYLDAKRTKLGHRLHHQERRFQEPGA"
BASE COUNT          819 a         2349 c         2473 g          864 t
ORIGIN      
        1 gcggcgcttg aacgcgtcca tggtctcgcg ccgctccgcc gcggggagct ggccgtggag
       61 gcagccgatc cggaacccgg agagctcggc atgtcgcagg cgctcggcct cctcggaggc
      121 ggcgcgcgcc tcgaccgcct cgctctccga gacgagtggg cagacgacgt aggcctgccg
      181 gccctcctcg aggtgcctgc gcaggcgccg gtacgcctcc gcgcttcgct cgtgcgtcac
      241 ccagcgggtc acgacgggct tgcggttccg gggcgggcgc gcgatctccg tcaccgcgag
      301 gtcgccgtag acgacctgcg cgagcgtccg cgggatcggc gtcgcggtca tgtggaggac
      361 gtgtggggcg cgcccgccgg ccgggccgct gatcgcccgg cgctgttcca ccccgaagcg
      421 gtgctgctcg tccacgaccg ccacggcgag cgagcggaag gcgacggcct cctggatgag
      481 cgcgtgcgtg ccgacgacga gctgcgcctc gccgctccct acgcggtcgc gcgcctcgcc
      541 gagcgcgcgg gcgccgagcg agctcgtcag gagcacggcc gcgacgccga cctcgcgcgc
      601 gagcggctcg accgtgagga agtgctgctc ggcgagcgtc tccgtcgggg ccatgagcgc
      661 gccctggtgg ccggcctcga ccgcgcggag gagcgcgtag agcgcaacga ccgtcttccc
      721 cgagccgacg tccccctgga ggagccgctg catcggcgcc tcgccggcga ggtcggcgcg
      781 gatcgcgtcg atcgccccct cctgctccgg ggtgagctcg aacggaagcg aggcgcgcaa
      841 cgcggccacg agcgcgccgt cctgcgcgcc gagcgcgtgc gcgcgtgcgc cctcctccgc
      901 cccgcgccgg cggcggacga ggccgacgag cagaatgagg agctcctcga acgcgagccg
      961 ctcgcgcgcg cgctcggcgt cctcgacgcg ctccggccgg tgggcgagga gcagggcgtc
     1021 cgcgcggagc ggcagccctt ccgcgtcgcg cacgccggcg ggaagcgcgt ccggccagtg
     1081 ccgggcgatt cggagcgccg cctccgtgac ctcgcggagc ttcgcggtcg agacgtcctc
     1141 gctcgccggg tagaccggga cgagcccgag cgtgtggagg ccggcgtcct ccgtgtcccg
     1201 ctcgtgccgc tcgacgcgga aggtcggcgg cccgaacccg ccctcgaggc ggccgtggag
     1261 caggacgcgc gtccccggcg gaagctggtc gacgagccac tgctggttga accagacggc
     1321 ggtgacgcga ctgccgttgt cgtcgcggac gctcgcctgc acgagcttga ggttccggcg
     1381 gcgcgtcggg cggacgccga tccgctcgat cgtgcagcgg acggtcgcct ccgttcccgc
     1441 gagcacctgg tcgaagccgc ccgcctcgct ccagtcgaga aacgcgcgcg ggaggtggtc
     1501 ggcgacgtcg cggacggtgc gcaggccgag ccgcgcgagc cgctgcgcga ccttcggacc
     1561 gacgccggga agcgtctcga ccgagacgtc cgggtcgggg aggcgcggtg ccctcggcgc
     1621 gtgccggtcc acgccgggga agccggctag cgccggacgg gtgctcaccg cgacgaccag
     1681 gacggtccga gtatcggagg cggtccggac ggcacgaggg cattgtcgcc gccgaagttt
     1741 cgacaaaccc ttgacaaggt atgaacgagt cctgtacggt ctttgtcgaa tgtttgtcta
     1801 accaaaggag ctcagtgatg aaaaggatct tcgcgttcgg gttgctcgtc ggcgcgctcg
     1861 ccctcgtggg cgtggcatgc ggcggcgacg acgacgcggc gacggacgag ccggccgccg
     1921 cgatggagga gacgacgacg atggaggagg agtcgaccga gacgaccgag accacgtcgc
     1981 aggagatgga catcgtcgag accgcggtcg cggcgggcag cttcgacacg ctcgtctcgc
     2041 tcgtccagga ggccgggctc gccgaggatc tctccggccc gggcccgctg acggtcttcg
     2101 cgccgacgga cgaggccttc gcggccgtcc ccgaggagac gctgacgcag ctccagaacg
     2161 acccggaggc gcttcgccgg gtgctcctct accacgtggt cgagggcgac gtcacctccg
     2221 accaggtgac cgggctcgac gcggcggcga cgctcgccgg ccccgacctg tccttctcga
     2281 cgaacggcgg cagcgtgctc gtgaacgacg cgacggtcac gatggccgac gtcctcgcct
     2341 cgaacggcgt gatccacgtc gtcgacgcgg tgcttctccc gcctgcccgc tagtacgcgg
     2401 gcacctcagg ccccggcgcg gccgtcgcgg tcgtgccggg gccgcctccc ctttctactc
     2461 ctcgtcgcgg aaccagaaga ggccgatcgt ccccggcccg ccgtgcgtgc cgatcaccgg
     2521 cccgagctgt gcctggaagt cgagcgaggc ctgcgggcgc agctcgcgga tgcggccgac
     2581 gagcgtctcg agctcggcct cggcgtccgc gtgcgcgacg cccacgtgga gcgcggggcc
     2641 gtcctccgtc ccctcctcga agagccgcag gagctcggcg agcgccttct ggcggccgcg
     2701 gacgcgcttg agcggctcga cctcgccctc gtcgatcgcg aggatcggct tcacggagag
     2761 gagctggccg gcgagccccg ccgccttccc gacgcgcccg ccgcgaacga ggtagtcgag
     2821 cgtgtcgacc gtgaagagga tcgtcgactc ccgctggaag cgggcgacga cctcctcgat
     2881 ctcctcgagc gtcgtgccgc ggtcgaggcg gcgctggacc gcgtcggcga ggatgacgac
     2941 gccgccggag acgctctccg agtcgatcac ggtgacgcgg tcgtccccga gcgcctgcgc
     3001 ggcgagccgc gcgctctcgg ccgtgccgga gagcttcccg gagatgagga cgcagaggat
     3061 ccgctcgaag ccctcgagct cggcgaaggc cgcctcgaag tcggcagggg tcggctggga
     3121 gctctgcggc tgcgcgtccg aggcgcgcag cttctcgtag aactcgaccg cgccgaggtc
     3181 gacgtggtcg cggtacgtct cgtcgccgaa gcggacgtag agcgggacga ggcgccagtt
     3241 cgggtggcgc gcggcggggt cggggagatc ggcggtcgag tcgaggacga ccgccgtcgt
     3301 ctcagacgag agacgcaccg cggctggagg cggaacggat cgccgcgacg atgtcgtcga
     3361 ggctcgagtc cttcgtcagg cacgccacgg cgcccgcgtc gtagagcgcg tcgagctccg
     3421 cgtcgtcggc cgtcgcggtc agcgcgacga ccgcggactc cgagcaggcg cgaatcgcgg
     3481 cggtcgcctc gacgccgtcg agctcgggca ggcggtagtc catgaggacg acgtcgggcc
     3541 gcagcagcgc gcacgcgtcg acggccgcgc ggccgtcgcc gaccgcgccg acgacccgga
     3601 cgtcctcgac gacgccgagc atgagctcga gcgcctcgcg gaagacctcg ttgtcctcga
     3661 cgaggaggac tgtggtcgac ggggcgtttt ccacaggttg agagtatcgg ccaaagagcg
     3721 cttgtggaaa agccccagtt tgggcggccg gtgacgctac tcggccgcga agagcagcgg
     3781 gtagtgcggc tggccgccct cgtggacgtc gatctcgagc gccgggtgcg cctcggcgag
     3841 ctcgtcgcgc aggccgttca cggacggggc ctcgtccccg aggaggatgg tgaggacgtc
     3901 cgcgcccggc tcgagcagat gggccgcgac ggcgcgcgcg acctcctcga cgctcgcccc
     3961 ggcggcgacg gcggctccgt ccgcgaggcc gaggaactcc ccttccgcga cctctatccc
     4021 gtcgatcgtg gccgcgcgcg acgcacgggc gaccgcgccg gagcggacgg ccgtcgcggc
     4081 ctcgcgcatc tcccgcgcgt tttcgtcgag cgccgccgtc gcgtcgaacg ggacgagcgc
     4141 ggcgagcccc gcctgcatcg acgtcgtctc caccacctcg acgggcttgc ccgcctccgc
     4201 ggcggcctgc tcggcggcga ggatcacgtt cttgttgttc gggaggagca cgacgccggc
     4261 cgcgtcgagg tcgtcgatcg cctggaggag ctgggaggtc ggcgggttca tcgtctgccc
     4321 gccctcgacg atcgccgccg cgccgaggtc gcggaagagc cgtgcgttcc cccctccggc
     4381 gacgaccgcg acgacggcgg tcgcggtcgg cccggcggtg cgcggcggcg ggtccgcgtg
     4441 cgtgatccgc tcctcgcgga cggccgtctg gacgtgcatg ttctcgatcg agacctcctc
     4501 gatcaccccc acggacgtgg cgagcgcgat cgcacggccc ggctcgtccg tgtggacgtg
     4561 caccttgacc gcgccgcgcg cgccgacgac gaggagcgag tcgccgaact cgccgagctc
     4621 gcgctcgagc gcgtccggct cggcgtcgtc gccctcgacg aagaagctcg tgcagtagcg
     4681 gtagcgcgag agctcccggt gaaccgcctc ctcggggagc gggcctgtgc cgacctcggg
     4741 ctcggggagc ggctcgccgc gcacgtgggc ggcgatcccg cggacgagct cgagcacgcc
     4801 ggcgccgccc gcgtcgacga cgcccgcgtc gcgcaggacg tcgagctgct cctgggtccg
     4861 ggcgagcgcg acctcgccgt gcgcgacgac ctccgcgagc gcgtcgccga gcgggagcgc
     4921 cgggtcgagc gcctcggcgc gctccgcgag ctcccgcgcg acggtgagca tcgtcccctc
     4981 ctgcggctcg cggaccgctg cgtacccggc gtccgacgcc ccgcgcaggc gcggcgcgag
     5041 gtcgcccgcg gtctccgggc cgccgttcag cccctcgacc gcgccgcgga cgagctgcga
     5101 gaggatgacg ccggagttcc cgcgggcgcc catgaggcac gagcgggcga cgagcgcgag
     5161 ctctgcctcc gcgtcgagct ccgtcgcgcc cgcgagggcg tcgcgaaccg cgcgcacggt
     5221 catcgtcatg ttcgtgccgg tgtccccgtc gggaacggga tagacgttca ggtcgtcgat
     5281 gcggcggcgg ctcgcttcga gcgcggcgag cgccgcttcg acgaccgtta gggcgccgcc
     5341 tggcacccgt cggagtctag tggcggccgc gctagacggc cttctgcacc ttgccgccct
     5401 tgaggcagcg ggtgcagacg tagctccggc gggcgacgcc gttcacgagg atccgaaccc
     5461 gctggaggtt cgggttgaag cggcgcttcg tggccaccat cgagtggctc cggttgtgac
     5521 cgaagccggg ggacttcccg cagacggcgc agaccttcga catcggccgc ggagtgtagc
     5581 gaaaccgatg cgcccgccta cgcggcgctc ggcccggggg cggcggtgac ggcgcgcagg
     5641 cgggcgaaga cgtcggccat ctcgagcgcg gagctcgcgg cctcctcccc cttgttcccg
     5701 tgcgccccgc cggcgcgggc ctcggcctgc gcgcggtcct cggtcgtgag gacgccgagg
     5761 acgaccggga cgccggtctc gagggcggcg agctgaaggc cgctcgcggc ctcggcggcg
     5821 atgtagtcga agtgcggcgt ctcgccgcgg atcacgcagc cgagcgcgac gacgcaggcg
     5881 tagcggcggc tcttcgcgag cgccatggcg ccgagcggga gctcgaacgc gccgggcacc
     5941 tggagaacgt cgatccggtc gcgctgcacg ccggccgcgg tgagcgcgcc gagcgcgccc
     6001 tcgaggagct tggtcgtgat gtcgccgttg tagcgggaga cgacgatccc taccgagcgg
     6061 cgcacgccgt tcggcatccc gacgaaggcg ccgacgccgt ccgggatcac gagccgcccg
     6121 ggcgcgtgct cggcgggcgg cgcggacgca gccggcgcgg caggcggatc ctcgacctct
     6181 cgctcgtccg gcgcgacgcg ggtctccgcc tccgcgtcga acgggggcgt cacccggagc
     6241 tcgtcggcgc cctcgaggcg gcccggcggg cgctcggtca cgcgccgggc tcctggaagc
     6301 gccgctcctg gtggtgcagg cggtgcccga gcttcgtccg cttcgcgtcg aggtagcgct
     6361 cgttctcggc attcggctcg atctcgatcg ggacctgctc ggtcacgcgg agcccgaagc
     6421 cctcgatgcc ggtgatcttg cgcgggttgt tcgtgaggat gcggatcgtc gtcaggccga
     6481 ggtcggcgag gatctgcgag ccgat
//
LOCUS       DASTFC010000010         7192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_511072,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000010 DASTFC010000000
VERSION     DASTFC010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511072"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..903)
                     /locus_tag="VFO88_00255"
     CDS             complement(352..903)
                     /locus_tag="VFO88_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543983.1"
                     /translation="MTENGGHSWAGLLRSYDVSEATATRLVSALAAAEVSALAFCRLL
                     DRWARGEPDPTTPGRREAALRRAAERVETALAGLERPLGRFLLELQADEAEGRSWYGE
                     PGAGEVVEWDATLRRAGVSASPARVAQAYLELAVLVRALQGLGTSARMGSLPHRSELW
                     AGLFDLRENLYGGAVDDLRALAA"
     gene            complement(942..2594)
                     /locus_tag="VFO88_00260"
     CDS             complement(942..2594)
                     /locus_tag="VFO88_00260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET9543984.1"
                     /translation="MSWTRIIPLGGLGEVGKNMTVVESEGGIVVIDAGLSFPRDEHLG
                     VDLILPDFGPLEDRRDEIRAVILTHGHEDHVGALPYFLREIGAPEIHATKLTLGLVKA
                     KLDEHGLGNAAELVEAEPEADPVQIGPFRVQFVRMAHSIPDSVAVVLDTPGGRIVHTG
                     DYKIDHTPVDGFRTDVGKLATLGNEGVDLLLGDSTNAERPGVTKSERTVGEAFRQIIP
                     RLEGRVLIASFASNVHRVQQAIDVALETDRKVCLIGRSLRKNVNIARSLGYVRVPDDL
                     LVKPNVLAELKPEETMILCTGSQGEPMSALTRIAFRDHQHVEIQEGDTVILSAKPVPG
                     NELRVHDTINRLAKGGATVLHEEIAPVHTSGHGNAEELRTILALLRPRNVMPVHGEFR
                     MLAAHARLAREAGIPEDRIVLAENGSVVELRGGILRLVDTLDIGSTFVDGLGVGDVRD
                     VALRDRRHLSEDGVLIVVVTVGSKNGRRAAAPEVIARGFSEPGPLFDEAREEVERTVT
                     ELLAQDIQELKLLQEHVHDAVGKLVYERTRRRPMILPVVVEV"
     gene            complement(2604..3503)
                     /gene="dapA"
                     /locus_tag="VFO88_00265"
     CDS             complement(2604..3503)
                     /gene="dapA"
                     /locus_tag="VFO88_00265"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9543985.1"
                     /translation="MLGEVLTAAVTPFRPDGSVNYETYADLCVHLVEHGSDGVVVAGT
                     TGESPTLTDDERLELVATAVDAVGDRATVIAGTGTYSTAHSVHLTERAHELGAHGFLV
                     VTPYYNKPPQRGIVEHFKAIAAASDRPIVVYNIPGRVVVNIEPETIAELAGIPTVAAV
                     KQANDDLGQARRIVEETRLALYAGDDNLIFPFLELGGVGGVCVHTHLWGPQVKEMIRL
                     HRAGDVEGARAIDRELAPAYELLKVATNPIAIKAALAMTGWELGRVRLPLVEADEGER
                     GRIRACLERGGVLSGTAAASARG"
     gene            complement(3513..3737)
                     /locus_tag="VFO88_00270"
     CDS             complement(3513..3737)
                     /locus_tag="VFO88_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543986.1"
                     /translation="MLAFRSEEHVDWWLAARRLPRGASFTLEQAWSLANGWYRDRLDP
                     AWQRKTPEEAEAVFAAAGLTGDFWRLRPSD"
     gene            complement(3744..4181)
                     /gene="merB"
                     /locus_tag="VFO88_00275"
     CDS             complement(3744..4181)
                     /gene="merB"
                     /locus_tag="VFO88_00275"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HET9543987.1"
                     /translation="MTDGDVRVHLMERFLAEGRPPSVAESAVALDASEEDVAAAYRRL
                     QEQRVIVLAPGTLDVWMAAPLSAVPTPFPVETERGTYYGNCIWDALGIPAMLGCDATI
                     AASCADCGDPLPIRVRNGELRDGDGVAHFAVPAARWWDNIGHT"
     gene            complement(4178..4822)
                     /locus_tag="VFO88_00280"
     CDS             complement(4178..4822)
                     /locus_tag="VFO88_00280"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET9543988.1"
                     /translation="MKVALFGASGKVGAVLAPRLRDAGHHVTPVERGEEPELAGHDAA
                     VDFTRPDAVRANVERALGAGLPALVGTTGLGEDDLAALDALAREHAVACLVVPNFALG
                     AVLMMRFAAEAARSLPRAEIVELHNERKVDAPSGTAKATAAAMGGDVPIHSVRLPGLV
                     AHQEVLLAGDGELLTIRHDTLSREAFVPGVLRALEGLRDLPPGVTVGLDAVLPA"
     gene            complement(4998..6290)
                     /locus_tag="VFO88_00285"
     CDS             complement(4998..6290)
                     /locus_tag="VFO88_00285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9543989.1"
                     /translation="MQSYLLTTLDGGGRVITEPLPSVRSAAIGIWIGSGSRDEDDSQA
                     GLSHFLEHLLFKGTKSYSALEIAEVFDGMGGELNASTARDYTVVYARVLDAHLDTALD
                     VMTDMVFAPTLADLDAEREVVLEEIAMIEDTPPELVHDLFAEAIFGDHPLGRPVLGSA
                     DVIASVPPADVAAYHRSRYRLDNVVVAAAGSVDHDALVERVRTMAPKRSRAGARTGAR
                     PPFVAPPPPSLRFVRKDTEQYHVCLGAPGLARSDRRRFTASILDGILGGSASSRLFQE
                     IREKRGLAYAVYTFGAQYADTGQIGVYVGTREENLAACMEVVAAELDALAAGGVREDE
                     VERAKENLKGRIMLSMEMTSNRMSRLGKSLVTDSELLSLDQILAEIDAVEASSVAGLA
                     AYLLAEERLSVAGIGPSEDRFLAAVDRITPGLVAAKAA"
     gene            complement(6338..>7192)
                     /locus_tag="VFO88_00290"
     CDS             complement(6338..>7192)
                     /locus_tag="VFO88_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9543990.1"
                     /translation="AGAELRDGVKVTGVEAGGGGVVVTAGGERLHGRTVVGADGVNGV
                     TARALGLGGNRSVDVALEGNVPNVRIDRSRYDGRLLMELGTVPGGYGWVFPKGDHVNV
                     GVGGDEREGPRLRAHLERLCRAHGLRIEELEDVRGYRLPHRAADSTLARGPALLAGDA
                     AGLVDPLTGDGMFEAFLSGRFAAEAVTALLAGEARTLDEYGARLSRRLAPHLWASWSF
                     RAALDRFPRTAYTLAKGRLVWPVVEKLVTGEIADVGAARGIVRAPLGALTLLGRAAGN
                     PGHAYKPA"
BASE COUNT          949 a         2692 c         2594 g          957 t
ORIGIN      
        1 attctccgct ccttccgtgg agatcgccgc tgcggcccgg cgatatgtgt gggcggggcg
       61 gatatcagat cgcggcgatc ggcacaagca gccgcaccgc tccgcgagaa cgccgcttcc
      121 cgaacgcggc cctaattgca ggcgtgttac gcctccggcg cggaagcgcg cgattcccga
      181 cccggggtct gaccccgtaa gcacgggcgc gtgacacttt cgtgacgggt tcgtgccgcc
      241 aagaaccttg ctccgagcag ggatgtcaca caatcggcac ggattctcag gcggtttggt
      301 gcggggtctg accccctcac gggcgccgac ccgctaagcg cgcgcgagta gctacgcggc
      361 gagcgcgcgg aggtcgtcga ccgcgccccc gtagaggttc tcgcggaggt cgaagagccc
      421 cgcccagagc tccgagcggt gcggcagcga gcccatccgt gcggacgtcc cgagcccctg
      481 gagcgcccgc acgagcaccg cgagctcgag gtacgcctgc gccacgcgcg ccggcgaggc
      541 gctcacgccc gcgcgccgaa gcgtcgcgtc ccactcgacg acctccccag cccccggctc
      601 gccgtaccac gagcggccct ccgcctcgtc cgcctgaagc tccaggagga agcgcccgag
      661 cggccgctcg agcccggcga gcgcggtctc gacccgctcg gctgcgcgcc ggagcgccgc
      721 ctcgcgccgg ccgggcgtgg tcggatcggg ctcgccgcgc gcccagcggt cgagcagccg
      781 gcagaacgcc agcgccgaca cctccgccgc ggcgagcgcc gagacgagcc gcgtcgcggt
      841 cgcctccgac acgtcgtacg agcgcaggag ccctgcccag gagtggccgc cgttctccgt
      901 catctcacta gtcttagcgg aagatcccgc gaacgggggc gtcagacctc gacgacgacg
      961 ggcaggatca tcggccggcg ccgcgtgcgc tcgtagacga gcttgccgac cgcgtcgtgg
     1021 acgtgctcct gcaggagctt cagctcctgg atgtcctgcg ccaagagctc ggtgaccgtt
     1081 cgctcgacct cctcgcgcgc ctcgtcgaag agcggccccg gctccgagaa gccgcgcgcg
     1141 atcacctcgg gcgccgcggc ccgccggccg ttcttgcttc cgaccgtcac gacgacgatc
     1201 aggacgccgt cctccgagag gtgccggcgg tcgcgcagcg cgacatcgcg cacgtcgccg
     1261 acgccgagcc cgtccacgaa ggtcgagccg atgtccagcg tgtccacgag ccgcaggatc
     1321 ccgccgcgca gctcgacgac ggagccgttc tccgcgagca cgatccggtc ctcggggatc
     1381 cccgcctcgc gcgcgagccg cgcgtgcgcc gcgagcatcc ggaactcccc gtgcaccggc
     1441 atgacgttgc gcggccggag cagcgcgagg atcgtcctga gctcctcggc gttcccgtgt
     1501 ccggacgtgt gcaccggcgc gatctcctcg tgcaggacgg tcgcgccgcc cttggcgagc
     1561 cggttgatcg tgtcgtggac gcgcagctcg ttccccggca ccggcttcgc ggagaggatg
     1621 accgtgtcgc cctcctggat ctcgacgtgc tggtggtcgc gaaacgcgat ccgggtgagc
     1681 gcggacatcg gctcgccctg gctccccgtg cagaggatca tcgtctcctc cggcttgagc
     1741 tcggcgagca cgttcggctt cacgagcagg tcgtcgggca cgcggacgta cccgagcgag
     1801 cgcgcgatgt tgacgttctt gcgcagcgag cggccgatga ggcacacctt ccgatcggtc
     1861 tcgagcgcga cgtcgatcgc ctgctgcacc cggtggacgt tcgaggcgaa ggacgcgatg
     1921 aggacgcggc cctcgagccg cgggatgatc tggcggaacg cctcaccgac cgtccgctcg
     1981 gacttcgtga cgcccggccg ctcggcgttc gtcgaatcgc cgagcaggag gtcgacgccc
     2041 tcgttgccga gcgtcgcgag cttcccgacg tccgtgcgga agccgtcgac cggcgtgtgg
     2101 tcgatcttgt agtcgcccgt gtgcacgatt cgcccgccgg gcgtgtccag gacgaccgcg
     2161 acggagtccg ggatcgagtg cgccatccgg acgaactgca cgcggaacgg cccgatctgc
     2221 acgggatccg cctccggctc ggcctcgacg agctccgcgg cgttcccgag cccgtgctcg
     2281 tcgagcttcg ccttcacgag cccgagcgtg agcttcgtcg cgtggatctc cggcgcgccg
     2341 atctcgcgca ggaagtacgg gagcgcgccg acgtggtcct cgtggccgtg cgtcaggatc
     2401 acggcccgga tctcgtcgcg ccggtcctcg agcggcccga agtccgggag gatgaggtcg
     2461 acgccgagat gctcgtcgcg ggggaacgag aggccagcgt cgatcacgac gatgccgccc
     2521 tcgctctcga cgacggtcat gttcttgccg acctcgccga gcccgccgag cgggatgatc
     2581 cgtgtccagg acatgtccgg agcctatccg cgcgccgatg ccgcggcggt gcccgagagc
     2641 acgccgccac gctcgaggca ggcgcggatc cgcccgcgct cgccctcgtc cgcctcgacg
     2701 agcggcagcc gcacgcggcc gagctcccag ccggtcatcg cgagcgcggc cttgatcgcg
     2761 atcgggttcg tcgcgacctt gagcagctcg taggcgggcg cgagctcgcg gtcgatcgcg
     2821 cgcgcgccct cgacgtcgcc ggcgcggtgg agccggatca tctccttcac ctgcgggccc
     2881 cagaggtgcg tgtgcacgca gacgccgccc acgccgccga gctcgaggaa cgggaagatg
     2941 aggttgtcgt cgcccgcgta gagcgcgagc cgcgtctcct cgacgatccg ccgggcctgc
     3001 ccgaggtcgt cgttcgcctg cttgacggcg gcgaccgtcg ggatcccggc gagctccgcg
     3061 atcgtctccg gctcgatgtt cacgacgacg cggccgggaa tgttgtagac gacgatcggc
     3121 cggtcgctcg cggccgcgat cgccttgaag tgctcgacga tcccccgctg cggcggcttg
     3181 ttgtagtacg gcgtgacgac gaggaagccg tgcgcgccga gctcgtgcgc gcgctcggtc
     3241 aggtgaaccg agtgcgcggt cgagtacgtg cccgtgcccg cgatcaccgt cgcgcggtcg
     3301 ccgacggcgt cgacggcggt cgcgacgagc tcgagccgct cgtcgtcggt gagcgtcggg
     3361 ctctcgccgg tcgtcccggc gacgacgacc ccgtcggagc cgtgctccac gaggtggacg
     3421 cagaggtcgg cgtacgtctc gtagttgacc gacccgtcgg gccggaacgg tgtgacggcc
     3481 gccgtcagga cttcgccgag cacgcgggca gtctagtcgc tcggccggag gcgccagaag
     3541 tcgccggtca gcccggcggc ggcgaagacg gcctcggcct cctcgggcgt cttccgttgc
     3601 caggccgggt cgaggcggtc gcggtaccac ccgttcgcga ggctccacgc ctgctcgagc
     3661 gtgaatgacg ccccgcgcgg gaggcggcgg gcggcgagcc accagtccac gtgctcctcc
     3721 gaccggaagg cgagcatcgt cgctcaggtg tggccgatgt tgtcccacca gcgcgcggcg
     3781 ggaaccgcga agtgagccac gccgtcgccg tcgcgcagct ccccgttgcg gacgcggatc
     3841 ggcagcgggt cgccgcagtc ggcgcagctc gccgcgatcg tcgcatcgca gccgagcatc
     3901 gcggggatcc cgagcgcgtc ccagatgcag ttgccgtagt acgtgccgcg ctcggtctcg
     3961 accgggaacg gggtcgggac ggcggagagc ggcgccgcca tccagacgtc gagcgtgccc
     4021 ggcgcgagga cgatcacacg ctgctcctgg aggcggcggt acgcggccgc cacgtcctcc
     4081 tcggacgcgt ccagcgcgac agccgactcg gcgacgctcg gcgggcggcc ctcggcgagg
     4141 aaccgctcca tgaggtgcac gcggacgtcg ccgtcggtca cgccgggagc accgcgtcga
     4201 gaccgaccgt cacgccgggc gggaggtcgc gcagtccctc gagcgcgcgg agcacgccgg
     4261 ggacgaacgc ctcacgggag agcgtgtcgt ggcggatcgt caggagctcg ccgtcgccgg
     4321 ctaggaggac ctcctggtgc gcgacgaggc cggggagccg cacgctgtgg atcgggacgt
     4381 cgccgcccat cgcggccgcg gtcgccttcg ccgtgccgga cggcgcgtcg accttgcgct
     4441 cgttgtgaag ctcgacgatc tcggcccgcg ggagggagcg tgcggcctcg gcggcgaagc
     4501 gcatcatgag gacggcgccg agcgcgaagt tcggcacgac gaggcacgcg acggcgtgct
     4561 cgcgcgcgag cgcgtcgagc gccgcgaggt cgtcctcgcc gaggcccgtc gtgccgacga
     4621 gcgccgggag gcctgcgccg agcgcccgct cgacgttcgc gcgcacggcg tcgggcctgg
     4681 tgaagtcgac ggcggcgtcg tggccggcga gctcgggctc ctccccgcgc tcgaccggcg
     4741 tcacgtggtg gccggcgtcg cgcaggcgcg gcgcgagaac ggcgccgacc ttcccggacg
     4801 cgccgaagag cgcgaccttc accgggcgcc cgcctgtgga cgatccggca cgcactcgtc
     4861 acttcccggc acacatccgc gcgatgttcc tcgatagcgt tcgcaaggga cgctcgctcc
     4921 cccatcgggg gggaacgccg cgcgggcgcg caccggccgt gcgccaacga gcgccgcgag
     4981 cggcgccgtc gccgcggtca tgcggccttc gccgccacga ggccgggcgt gatccggtcg
     5041 acggcggcga ggaagcggtc ctcgctcggc ccgatcccgg cgacggacag gcgctcctcg
     5101 gcgaggaggt acgcagcgag gcccgccacc gacgacgcct cgaccgcgtc gatctcggcg
     5161 aggatctggt cgagcgagag cagctcgctg tccgtcacga gcgacttgcc gagccggctc
     5221 atccggttcg acgtcatctc catcgagagc atgatccggc ccttgaggtt ctccttcgcc
     5281 cgctcgacct cgtcctcgcg cacgcccccg gcggcgaggg cgtcgagctc cgcggccacc
     5341 acctccatgc acgccgcgag attctcctcg cgtgtcccga cgtacacgcc gatctgcccc
     5401 gtgtccgcgt actgcgcgcc gaacgtgtag acggcgtacg cgaggccccg cttctcccgg
     5461 atctcctgga agagccgtga cgacgccgac ccgccgagga tcccgtccag gatcgacgcg
     5521 gtgaaccgcc gccggtcgga gcgcgcgagc cccggcgcgc cgaggcacac gtggtactgc
     5581 tccgtgtcct tgcggacgaa ccggagcgac ggcggcggcg gcgcgacgaa cgggggccgc
     5641 gcgcccgtcc gggcgcccgc gcgcgaccgc ttcggcgcca tcgtgcgcac ccgctcgacg
     5701 agcgcgtcgt ggtcgacgct ccccgcggcg gcgacgacga cgttgtcgag ccggtagcgc
     5761 gagcggtggt acgcggccac gtccgcaggc ggcaccgacg cgatcacgtc cgccgacccg
     5821 agcacgggcc ggccgagcgg atggtcgccg aagatcgcct ccgcaaagag gtcgtgcacg
     5881 agctcgggcg gcgtgtcctc gatcatcgcg atctcctcga gcaccacctc ccgctccgcg
     5941 tcgaggtcgg cgagcgtcgg cgcgaagacc atgtcggtca tcacgtcgag cgcggtgtcg
     6001 aggtgcgcgt cgagcacccg cgcgtagacg acggtgtagt cgcgcgcggt cgacgcgttc
     6061 agctcgccgc ccatcccgtc gaagacctcg gcgatctcga gcgcggagta cgacttcgtc
     6121 cccttgaaga gcaggtgctc gaggaagtgc gagaggccgg cctgcgagtc gtcctcgtcg
     6181 cgcgagccgg agccgatcca gatcccgatc gccgcggagc gcacgctcgg aagcggctcc
     6241 gtgatcaccc ggccaccccc gtcgagcgtc gtcaggaggt agctctgcac gctcgcatgg
     6301 tactccccgc ccggcccgcc tcctgcccgc ggggcgacta ggccggcttg tatgcgtgcc
     6361 ccgggttccc ggccgcccgg ccgagcagcg tgagcgcccc gagcggcgcc cggacgatcc
     6421 cgcgggccgc gccgacgtcc gcgatctcgc ccgtgacgag cttctcgacg accggccaga
     6481 cgagccgccc cttcgccagc gtgtacgccg tgcgcgggaa ccggtcgagc gcggcgcgga
     6541 agctccacga cgcccagagg tgcggggcga gccggcggga gaggcgcgcg ccgtactcgt
     6601 ccagcgtccg cgcctcgccg gcgaggagcg ccgtgaccgc ctcggcggcg aagcggccgc
     6661 tcaggaaggc ctcgaacatg ccgtcgcccg tgagcgggtc gacgagcccc gccgcgtcgc
     6721 ccgcgaggag cgccgggccg cgcgcgagcg tcgagtccgc cgcgcggtgc gggagccggt
     6781 agccgcgcac gtcctcgagc tcctcgatcc ggagcccgtg cgcgcggcag agccgctcga
     6841 ggtgcgcgcg caggcgcggc ccctcgcgct cgtcgccgcc caccccgacg ttcacgtggt
     6901 cgcccttcgg gaagacccag ccgtagccgc ccggcaccgt gccgagctcc atgagcagcc
     6961 ggccgtcgta gcgggagcgg tcgatccgca cgttggggac gttcccctcg agcgcgacgt
     7021 cgaccgagcg gttcccgccg agcccgagcg cccgggcggt gacgccgttc accccgtcgg
     7081 cgccgacgac cgtccggccg tgcagccgct cgccgcccgc ggtcacgacg acgccgccgc
     7141 cccccgcctc gacgcccgtc accttcacgc cgtcgcggag ctccgccccg gc
//
LOCUS       DASTFC010000011         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_66072,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000011 DASTFC010000000
VERSION     DASTFC010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66072"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFO88_00295"
     CDS             complement(<1..473)
                     /locus_tag="VFO88_00295"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9543991.1"
                     /translation="MFSRSAHLYDAIYSFKDYAGEAETLRALVEERAPGARSLLDVAC
                     GTGTHLAELRRWYDVEGVDVDPALLAIARERLPDVPLHEGDMVTLELGRAFDVVTCLF
                     SSIAYVVTPERLAAATAALARHVAPGGLLVVEPWILPEQWAPDHIGAVFVDEPEL"
     gene            528..863
                     /locus_tag="VFO88_00300"
     CDS             528..863
                     /locus_tag="VFO88_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543992.1"
                     /translation="MYETLERRATTLVLVTGLVVPLTALSAGGFFEGSDLSAALTFAA
                     LGLLTIGLALGVAALFPAERTDRLSMKAQLVFWAHAAFAAGVVLVAANTAYLTYRLLR
                     ADPDFFGLG"
     gene            complement(860..>2362)
                     /locus_tag="VFO88_00305"
     CDS             complement(860..>2362)
                     /locus_tag="VFO88_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9543993.1"
                     /translation="EHDYDLAVLAQWDGRRRYANLRKLGRLARSYESLRGPDLEGFVR
                     FLRDQEAAGAREVEAVAEEEGAEAVRLLTVHAAKGLEFKVVVLADAGRVPPSPAADEI
                     LCLPDGRFGFRVVNPATGRRAGAFGWDDMRDAEQTADQAENRRLLYVAMTRAIDRLIV
                     SGAVDATSRRDGNAPLRWILGRLEIDLDGDGRQELERGGARLVLEVHRPGEEEGARAE
                     EAAPQLQLFAPVENGGGPRPALELPPLAEVPAPPANALRRLSFSALALYGRCSYRFYA
                     ERFVGLRPADDVRAVAGVEGLAATELGDVVHVLLEHGVAAEDVRARALERYPSATPDD
                     LDRIEALVAAWHGSALARRLAVAEGARPELPFAFEHDGVLIHGRFDLFELADGRALVV
                     DYKTNRLEGLDPADALEEGYTLQRLVYALAAFRAGAEEVEVAYAFLERPDEPVVTTFS
                     REDAAGLEAELTEAIRRIREGEFRPTPGELACPGCPALDVVCAGPRLLEP"
BASE COUNT          305 a          931 c          831 g          295 t
ORIGIN      
        1 ttcagctccg gctcgtcgac gaagacggcg ccgatgtggt cgggagccca ctgctccggc
       61 aggatccacg gctcgaccac gaggagcccg cccggcgcga cgtgacgcgc gagcgccgcc
      121 gtcgcggccg cgagccgctc cggcgtcacg acgtacgcga tcgacgagaa gaggcacgtg
      181 acgacgtcga acgcccggcc gagctcgagc gtgaccatat cgccctcgtg gagcggaacg
      241 tccggcagcc gctcacgcgc aatggccagg agcgccggat ccacgtccac gccctccacg
      301 tcgtaccagc ggcgcagctc cgcgaggtgc gtccccgtcc cgcaggcgac gtcgagcagc
      361 gaccgggccc cgggcgcgcg ctcctcgacg agcgcgcgca gcgtctccgc ctcgcccgcg
      421 tagtccttga aggagtagat cgcgtcgtag aggtgcgccg agcgcgagaa cacggcgaga
      481 ggctacgcca gcccgtcgac gtccgccctc cttcgtaggc tcaccgcgtg tacgagacgc
      541 tcgaacggcg cgcgaccacg ctcgtcctcg tcaccgggct cgtcgtcccg ctcacggcgc
      601 tcagcgcggg cggcttcttc gagggctcgg acctgtccgc cgctctcacg ttcgcggcgc
      661 tcgggctcct gaccatcggc ctcgcgctcg gggtcgcggc gctcttcccg gcggagcgaa
      721 ccgaccggct gtccatgaag gcccagctcg tgttctgggc gcatgcggct ttcgcggccg
      781 gcgtcgtcct cgtggccgcc aacaccgcat atctgaccta ccggctcttg agggccgacc
      841 cggacttctt cggcctcggc tagggctcga gcaggcgcgg ccccgcgcag acgacgtcga
      901 gggcggggca gcccgggcat gcgagctcgc ccggggtcgg gcggaactcg ccctcgcgga
      961 tccgccggat cgcctcggtc agctcggcct cgagcccggc cgcgtcctcg cgcgagaacg
     1021 tcgtcacgac cggctcgtcg ggccgctcga ggaacgcata cgcgacctcg acctcctccg
     1081 ccccggcgcg gaacgccgcg agcgcgtaga cgaggcgctg gagcgtgtag ccctcctcga
     1141 gcgcgtcggc cgggtcgagc ccctcgagcc ggttcgtctt gtagtcgacg acgagtgccc
     1201 tgccgtccgc aagctcgaag aggtcgaacc ggccgtggat caggacgccg tcgtgctcga
     1261 acgcgaacgg gagctccggc cgagcgccct cggcaaccgc cagccgtcgc gcgagcgccg
     1321 agccgtgcca ggcggcgacg agcgcctcga tccggtcgag gtcgtccggc gtcgccgacg
     1381 gataccgctc gagcgcccgc gcgcggacgt cctctgccgc cacgccgtgc tcgagcagga
     1441 cgtggacgac gtcgccgagc tccgtcgccg cgagcccctc cacgccggcg accgcccgca
     1501 cgtcgtccgc cggccgcaac ccgacgaacc gctcggcgta gaagcggtac gagcagcgcc
     1561 cgtagagcgc gagcgcgctg aaggagagcc gccggagcgc gttcgcgggc ggcgccggca
     1621 cctccgcgag cggcggcagc tcgagcgccg gccgcggccc gccgccgttc tccaccggcg
     1681 cgaagagctg gagctgcggc gcggcctcct ccgcccgggc tccttcctcc tcgcccggcc
     1741 ggtgcacctc gagcacgagc cgcgcaccgc cgcgctcgag ctcctgccgc ccgtccccgt
     1801 cgaggtcgat ctcgagccgc ccgaggatcc accgcagcgg cgcgttcccg tcccgccggc
     1861 tcgtcgcgtc gaccgcgccc gagacgatca gccggtcgat cgcgcgcgtc atcgccacgt
     1921 agaggaggcg gcggttctcc gcctggtcgg cggtctgctc ggcgtcgcgc atgtcgtccc
     1981 agccgaacgc gcccgcacgc cgcccggtgg ccgggttcac gacgcggaat ccgaagcgcc
     2041 cgtcgggcag gcacaggatc tcgtcggcgg ccggcgaggg cggcacgcgg cccgcgtcgg
     2101 cgaggacgac aaccttgaac tcgagcccct tcgcggcgtg gacggtcagg agccgcaccg
     2161 cctcggcgcc ctcctcctcc gcgaccgcct cgacctcgcg cgcgccggcc gcctcctggt
     2221 cgcgcaggaa gcggacgaac ccctcgaggt ccgggccgcg cagcgactcg tacgagcgcg
     2281 cgagccggcc gagcttgcgg aggttcgcgt agcgccgccg cccgtcccac tgggcgagca
     2341 cggctaggtc gtagtcgtgc tc
//
LOCUS       DASTFC010000012         5800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1384490,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000012 DASTFC010000000
VERSION     DASTFC010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384490"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFO88_00310"
     CDS             <1..717
                     /locus_tag="VFO88_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9543994.1"
                     /translation="ESVRRKPYSVVLLDEVEKAHPDVFNILLQILEDGKLTDAQGRKV
                     DFRNTIVIMTSNIGAATISKNQSFGFGMGDETGLSYDDMKARIMGELKKVFRPELLNR
                     IDEVIVFHKLTKDEIKTIVEFMLKRLREQMGEHEAVIELTEDAKELLVEKGYDPAMGA
                     RPLRRAIQRHIEDPLADFVLGRTLKPGATIVVDRKAGTEDVDISIVEGPEPGPEREKV
                     TVPPEEPEGSADGDAPAEEE"
     gene            770..1711
                     /locus_tag="VFO88_00315"
     CDS             770..1711
                     /locus_tag="VFO88_00315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9543995.1"
                     /translation="MDTFVSVCLPAFNGAAYIGEAIDSVLAQTHERFELVVVDNNSSD
                     GTRDVVGGYDDERIRLVVNDVTVGAPENHNLAVRAARGQLVKFLHHDDRLAPTCLERL
                     VPVFEEATSVGLAFSRRDVLVDPADADAVAWKERYGTVHEPLGPLGRVNDGPTLLARY
                     VEGLGGPLLPNWIGEPSCVMVRMGALDRAGLFTTRMRQSWDVELWLRIMATWDVGFVD
                     EPLALYRYHGASLTQANARGNEDWLDLLWLHEGLLSAPELESVAGTLRAHRRRELARS
                     PRRALGRLARRRGIGGLVGYAGYRAGRAVGRAPALHG"
     gene            complement(1719..2396)
                     /locus_tag="VFO88_00320"
     CDS             complement(1719..2396)
                     /locus_tag="VFO88_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9543996.1"
                     /translation="MDKFNALSRGAQLMLVGAALLLVDTFLPWQSVSFEALGISVSES
                     WTAWHGFWGVLLGLLTVALLAWLVLRLVGTEIALPVSDALVAAALAGLILLSALLKVL
                     TDDAVAWGAWLGLALAVVIAVGAWLQVQDAGGADTLWNEASAAGGGSGAAAGGADEPM
                     SASAPPSAPVTPATPPAAPAEPAAPAPPAEPVAPAEAGAPTAEEEPPAPVPPPPPDTT
                     PSDEPRQ"
     gene            2537..3901
                     /gene="radA"
                     /locus_tag="VFO88_00325"
     CDS             2537..3901
                     /gene="radA"
                     /locus_tag="VFO88_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET9543997.1"
                     /translation="MAKAAAVQYACSECGYATGKWLGRCPACEGWGTLVEERAAAPTG
                     RSGSGAARGKPVLRLVDVEAADAERLTTGVPELDRVLGGGLVPASLVLVSGDPGIGKS
                     TLLLMALRALAEERRVVLVTGEESTAQVKLRADRLGGAGDVGILAETNLDEVCATLER
                     ERPEVAVIDSVQTLYSPELGSAPGSVGQVREAAARLLRLAKEEGIAVFLVGHVTKDGS
                     VAGPRVLEHLVDCVLQFEGDRYRSHRILRAVKNRFGSTNELGIFEMTGQGLVGVEDPS
                     LLFGRPEDEQPGSAVACALEGTRPLVLEIQALVSKTDLAMPRRVATGVDPKRLSMIVA
                     VLGRHAGVPLGQADVFVNVAGGVRIDEPGADLAVALAIASAARGVPVRRGVACLGEIG
                     LTGRLRPVAQLERRLAECEKLGLGEAIVPEGAGAHGRMRTSAADTLRRAIETGLDAGG
                     PSEG"
     gene            3876..4961
                     /gene="disA"
                     /locus_tag="VFO88_00330"
     CDS             3876..4961
                     /gene="disA"
                     /locus_tag="VFO88_00330"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HET9543998.1"
                     /translation="MRADPRKDKTLLTALAQVAPGTDLRSGIDDVIRSQHGALVVIGD
                     PNELAFLFSGGMRLDLPLTPQLLYELAKMDGAIIVNGDLSKLVYANVQLMPDPTIPSA
                     ETGTRHRTAERVAKQTGTLVISISQQRETVTLFVGDLRYQLGEISEVLARTNQGLATL
                     ETYKSRLDQGLTRLSALEFQSAVMLDDVLVVLQRAEMAMRMVEEIERNRVELGEEGRL
                     IEMQLEELVGDVPRERAAVVYDYELTGDPAAAVAALERLSALPYERLSELEEIGGLLG
                     YPRDANPLDFTVSPRGYRILSQIPRLSEGIVRHVVRDLGSLEAVIRASQRELEAVEGV
                     GAVRAREIREGLRRLQEHNLVDRYLQL"
     gene            5128..5634
                     /locus_tag="VFO88_00335"
     CDS             5128..5634
                     /locus_tag="VFO88_00335"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET9543999.1"
                     /translation="MYKVGDKVVYPHHGAGVVAKKESRTILGQEREYLTIKILHNDMT
                     VNVPSENAERVGLRKVIDEEMVERVLEVLHGDGTKMPKNWNRRFKYNRDKMKTGDIFE
                     LAEVVRNLSLRDQEKGLSTGEKQMFVKAKKILVSELMYAKGMDEDETAAWLDEVLGAP
                     VREPVATS"
BASE COUNT          848 a         2026 c         2144 g          782 t
ORIGIN      
        1 gagagcgtcc gccgcaagcc gtactcggtc gtcctcctcg acgaggtcga gaaggcccac
       61 ccggacgtct tcaacatcct cctccagatc ctcgaggacg ggaagctgac ggacgcccag
      121 gggcgcaagg tcgacttccg caacacgatc gtgatcatga cctcgaacat cggcgccgcc
      181 acgatctcga agaaccagtc gttcgggttc gggatgggcg acgagacggg cctctcgtac
      241 gacgacatga aggcccggat catgggcgag ctcaagaagg tcttccggcc ggagctcctg
      301 aaccgcatcg acgaggtcat cgtcttccac aagctcacga aggacgagat caagacgatc
      361 gtcgagttca tgctgaagcg cctgcgcgag cagatgggcg agcacgaggc ggtgatcgag
      421 ctcaccgagg acgcgaagga gctcctcgtg gagaagggct acgacccggc gatgggcgcc
      481 cggccgctgc gccgcgcgat ccagcgccac atcgaggacc cgctcgccga cttcgtgctc
      541 gggcggacgc tcaagcccgg cgcgaccatc gtcgtggacc gcaaggccgg cacggaggac
      601 gtggacatct cgatcgtcga gggccccgag ccgggcccgg agcgcgagaa ggtcacggtc
      661 ccgcccgagg agcccgaggg ctccgcggac ggcgacgcgc cggccgagga ggagtagggc
      721 ccggcgcctc ccgcgacgcc tggcgcgggc ggcgttcggt cacctcggca tggacacgtt
      781 cgtctcggtc tgcctgcccg cgttcaacgg cgcggcctac atcggcgagg cgatcgactc
      841 cgtgctcgcg cagacgcacg agcggttcga gctcgtcgtc gtcgacaaca actcgtcgga
      901 cgggacgcgc gacgtcgtcg ggggctacga cgacgagcgg atccggctcg tcgtcaacga
      961 cgtcacggtc ggcgcaccgg agaaccacaa cctcgccgtg cgcgccgcgc gcgggcagct
     1021 cgtcaagttt ctccaccacg acgaccggct ggcgcccacc tgcctcgagc ggctcgtgcc
     1081 ggtgttcgag gaggcgacga gcgtcgggct cgcgttctcg cgccgggacg tgctcgtcga
     1141 cccggcggac gccgacgcgg tcgcgtggaa ggagcgctac ggcacggtgc acgagccgct
     1201 cggaccgctc gggcgcgtga acgacgggcc cacgctcctc gcccgctacg tcgaggggct
     1261 cggcgggccg ctcctgccga actggatcgg cgagccgtcg tgcgtgatgg tgcgcatggg
     1321 cgcgctcgac cgggcgggcc tcttcacgac gcggatgcgg cagtcgtggg acgtcgagct
     1381 ctggctgcgg atcatggcca cctgggacgt gggcttcgtc gacgagccgc ttgcgctcta
     1441 ccgctaccac ggggcctcgc tcacgcaggc gaacgcccgc ggcaacgagg actggctcga
     1501 cctgctctgg ctccacgagg ggctgctctc cgcgcccgag ctcgagtcgg tcgcagggac
     1561 gctacgcgcg catcgccggc gcgagctcgc gcgctccccg cgccgtgcgc tgggccggct
     1621 ggcgcgccgg cgcgggatcg gcggcctcgt cggctacgcg ggctaccgcg cgggccgggc
     1681 cgtcggccgg gcgccggccc tgcacggcta gcccgcggtc actgcctcgg ctcgtccgag
     1741 ggggtcgtgt ccggcggagg cggcggcacg ggagcgggcg gctcctcctc ggcggtcggc
     1801 gcgcccgcct cggccggggc gaccggttct gcgggcggtg cgggcgcggc aggctcggct
     1861 ggagcggccg gcggggtggc cggggtgacc ggcgcggacg gtggcgccga ggccgacatg
     1921 ggctcgtccg caccccccgc tgccgcgccc gatccgccgc cggccgccga ggcctcgttc
     1981 cacagcgtgt cggcgccgcc ggcgtcctgc acctggagcc aggcgccgac cgcgatcacg
     2041 accgcgagcg cgagcccgag ccacgcgccc caggcgaccg cgtcgtccgt cagcaccttg
     2101 agcagcgccg acaggaggat cagccctgcg agcgcggcgg cgacgagcgc gtccgagacc
     2161 ggtagcgcga tctccgtccc gacgagccgc aggacgagcc acgcgagcag ggcgaccgtc
     2221 agcaggccga ggaggacgcc ccagaagccg tgccaggcgg tccacgactc ggagacgctg
     2281 atcccgagcg cctcgaagga gacgctctgc cacgggagga acgtgtccac gaggaggagc
     2341 gccgcgccca cgagcatgag ctgcgccccg cgggagagcg cgttgaactt gtccatggcg
     2401 atcgtccttt ctcggcctgc ggccggggtt tcccggaacg atctccgaca cggcggacgg
     2461 tgggcgggcg tttcggcacg caatcggcac ggtcgcgcgc ggaccgccgt cgggcgcgcg
     2521 gcctagactc gcttccgtgg cgaaggcggc ggccgtccag tacgcatgct ccgagtgcgg
     2581 ctacgcgacc gggaaatggc tcgggcgctg ccccgcgtgc gagggctggg ggacgctcgt
     2641 ggaggagcgc gcggccgccc cgaccgggcg ctccggctcc ggcgccgcgc ggggcaagcc
     2701 ggtgctccgc ctcgtcgacg tcgaggcggc cgacgccgag cgcctcacga cgggcgtgcc
     2761 ggagctcgat cgcgtgctcg gcggcgggct cgtcccggcg agcctcgtcc tcgtctcggg
     2821 cgacccgggg atcggcaagt cgacgctgct cctcatggcg ctgcgggcgc tcgcggagga
     2881 gcgccgggtc gtgctcgtca cgggcgagga gtcgaccgcc caggtgaagc tccgcgccga
     2941 ccggctcggc ggcgccggcg acgtcgggat cctcgcggag acgaatctcg acgaggtctg
     3001 cgcgacgctc gagcgggagc ggccggaggt cgcggtgatc gacagcgtgc agacgctcta
     3061 ctcgcccgag ctcggctcgg cgcccgggtc ggtcggtcag gtgcgcgagg ccgccgcgcg
     3121 gctgctccgc ctggcgaagg aggagggcat cgccgtcttc ctcgtcggcc acgtaacgaa
     3181 ggacggctcg gtcgcgggcc cccgcgtgct cgagcacctc gtcgactgcg tgctccagtt
     3241 cgagggcgac cgctaccgct cgcaccggat tctccgcgcg gtcaagaacc gcttcggctc
     3301 gacgaacgag ctcgggatct tcgagatgac cgggcagggg ctcgtcggcg tcgaggaccc
     3361 gtcgctcctc ttcgggcgcc ccgaggacga gcagcccggc tcggccgtcg cgtgcgcgct
     3421 cgagggcacg cgcccgctcg tgctcgagat ccaggcgctc gtctcgaaga ccgacctcgc
     3481 gatgccgcgg cgcgtcgcca ccggcgtcga cccgaagcgg ctctcgatga tcgtcgcggt
     3541 tctcgggcgc cacgcgggcg ttccgctcgg gcaggcggac gtcttcgtga acgtcgccgg
     3601 cggggtgcgg atcgacgagc ccggggccga cctcgccgtc gcgctcgcga tcgcctccgc
     3661 ggcccgcggc gtgccggtgc ggcgcggcgt cgcctgcctg ggtgagatcg gcctgaccgg
     3721 ccggttgcgc ccggtcgcgc agctcgagcg ccggctcgcg gagtgcgaga agctcggcct
     3781 cggggaggcg atcgtgccgg agggcgccgg ggcgcatggc agaatgcgca cgagcgccgc
     3841 ggacaccctg cgtcgcgcga tcgaaacggg gctcgatgcg ggcggaccct cggaaggata
     3901 agaccctcct cacggcgctc gctcaggtgg cgccgggaac ggatctgcgc tccgggatcg
     3961 acgacgtcat ccgctcgcag cacggggcgc tcgtcgtgat cggcgacccg aacgagctcg
     4021 cgttcctctt ctcgggcggg atgcggctcg acctgccgct cacgccccag ctcctgtacg
     4081 agctggccaa gatggacggg gcgatcatcg tgaacggcga tctctcgaag ctcgtctatg
     4141 cgaacgtcca gctcatgccc gacccgacga tcccgtcggc cgagaccggg acacgacacc
     4201 gtacggccga gcgcgtcgcc aagcagacgg ggacgctcgt catttcgatc tcgcagcagc
     4261 gcgagaccgt cacgctcttc gtcggcgacc tgcgctacca gctcggcgag atctccgagg
     4321 tcctcgcgcg gacgaaccag gggctcgcca cgctcgagac gtacaagtcg cggctcgacc
     4381 aggggctcac gcggctctcg gcgctcgagt tccagagcgc ggtcatgctc gacgacgtcc
     4441 tcgtcgtcct ccagcgcgcc gagatggcga tgcggatggt cgaggagatc gagcggaacc
     4501 gggtcgagct gggcgaggag ggccggctga tcgagatgca gctcgaggag ctcgtcggcg
     4561 atgtcccgcg cgagcgcgcc gcggtcgtct acgactacga gctgaccggc gaccccgcgg
     4621 cggcggtcgc ggcgctcgag cggctctcgg cgcttccgta cgagcggctc tcggagctcg
     4681 aggagatcgg tgggctgctg ggctacccgc gcgacgccaa cccgctcgac ttcaccgtct
     4741 cgccgcgcgg gtaccggatc ctctcgcaga tcccgcggct ctccgaggga atcgtccgcc
     4801 acgtcgtccg cgacctcggc tcgctcgagg cggtcatccg cgcgagccag cgcgagctcg
     4861 aggccgtcga gggggtcggc gccgtgcgcg cgcgggagat ccgagagggc ctccggcgcc
     4921 tccaggagca caacctcgtc gaccgctacc tccagctctg agcggcctgc cgcgtcacga
     4981 aaaagtcctg ctcagagcgg gctcgcccga ctttgtgacg acaaaatccc tggaatattg
     5041 gcttttttcg ctccagatcg gtacaattgc gcaggttgaa gaggcgttcc gggtgcacag
     5101 gcaccccttt ttctagaggg aggcagcttg tacaaagtcg gcgataaggt cgtctacccc
     5161 caccacggcg caggggtcgt tgcgaagaag gagagccgca cgatcctcgg tcaggagcgg
     5221 gaatacctca cgatcaagat ccttcacaac gacatgacgg tgaacgtccc gtccgagaac
     5281 gcggagcggg tcgggctccg gaaggtgatc gacgaggaga tggtcgagcg cgtcctcgag
     5341 gtgctgcacg gggacggcac gaagatgccg aagaactgga accgccgctt caagtacaac
     5401 cgcgacaaga tgaagaccgg cgacatcttc gagctcgccg aggtcgtccg gaacctgtcg
     5461 ctccgcgacc aggagaaggg cctctcgacc ggcgagaagc agatgttcgt gaaggcgaag
     5521 aagatcctcg tgtccgagct catgtacgca aagggcatgg acgaggacga gaccgccgcc
     5581 tggctcgacg aggtgctcgg cgctcccgtg cgggagcccg tcgccacgag ctgacgtcgg
     5641 gcgcggacgt caccggcgtc tgggcggtcc tcgtcgccgc cggccgcggc gatcggctgg
     5701 gcggcgagaa cccgaaggcg ttcgcagggc tggggggccg gccgctcctc gccgagagcg
     5761 tggagcggct cgacgcgtcc gagtgggtcg aggcgatcgt
//
LOCUS       DASTFC010000013         7535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_445141,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000013 DASTFC010000000
VERSION     DASTFC010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445141"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFO88_00340"
     CDS             <1..408
                     /locus_tag="VFO88_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544000.1"
                     /translation="FPGTLLLVSHDRALLDAIAERTLAIEDGRVRSYEGGWADYVRAR
                     AERVAPPEPPVEPVPARRPRTPKPAAAKKGPDPLVELEREIEAQERVVARLEAELADD
                     WTNIDTIAAHRRAREDLQRLFARWEELLDRAPS"
     gene            458..943
                     /locus_tag="VFO88_00345"
     CDS             458..943
                     /locus_tag="VFO88_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544001.1"
                     /translation="MPFAVLEAATEGYAEGEEPLAWLLVASLALAAVALPLAGRSGRP
                     AWLGAGALVFDTAFLSAWAVLYAAEPGTPARELLVLAAVEAGLRYGPRGALWALATLP
                     ALVLFELRLSNTLDAPFDVGHAVFPAGLYGLVGLVVGALSEERRRGGSPAPPAEPPSS
                     A"
     gene            complement(879..1967)
                     /locus_tag="VFO88_00350"
     CDS             complement(879..1967)
                     /locus_tag="VFO88_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HET9544002.1"
                     /translation="MSVLDDPNAPPLRVLELRDDAVGLEAFIVVDHELFPRSAGGTRM
                     VPDVTVDEVARLARAMTWKFGVAHVPLAGAKAGIRFPGGDAAARERVLSAYVDRILAM
                     RDVFGTGPDMGTHPSDFLRLAPEGRTPMWARTHDGMGMDDLAVGAGILGAARAALAAL
                     GRPLEGARAAIEGFGKAGAGTAHALARAGARIVGVSTIEGALLDPEGLDVDVLLALKA
                     EHGDRLVHHAGVPVRPREELAAVECDVLVPGARPDFVTVDTAPLLRCAAIVPAANIPY
                     GPGAVEALHARGILALPDFVANAGGVHLYEAPGCEGDDPAACLAEVERLIREASERVL
                     AAAEADGVTPTKAAQRVALDFLRAAAPR"
     gene            complement(1982..2554)
                     /locus_tag="VFO88_00355"
     CDS             complement(1982..2554)
                     /locus_tag="VFO88_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9544003.1"
                     /translation="MRILVAEDETIIRLDLRTTLEEAGFEVCAEARNGEEAVELAASE
                     RPDLAILDVKMPRLDGIEAARRILAERPIPIVMLTAYGQEELVARAVEAGVFGYLVKP
                     FREADLLPAIHAARARHAELEALREEAESLAEALATRKVVERAKGLLMEREGLSEEEA
                     FGRLRRASQSSNRPMKVVAEAIVATFADES"
     gene            complement(2551..3969)
                     /locus_tag="VFO88_00360"
     CDS             complement(2551..3969)
                     /locus_tag="VFO88_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9544004.1"
                     /translation="MSETARDASARHLRLITETIAAVNSTLDLGEVFELVAAKVAEAL
                     EADACFVYLYDEAADELVLRASYGTRVEDMTRVPKMRPGEGITGTAAAERAPVMIPRA
                     AHLDERFKAFPNLREEQYESILAVPILARDRLEGALNVRTQEPREFSDAEIALLMAIA
                     GQVAQAIENAKLYDAAQRRVAELEALAKISEAVSESLYLEESLGAIVETTMAAVGATG
                     AALVLEDGRIAWPEGRAGAHAVRLPLRWKGREIGELVCDRDTPFSTDERRLLASIASQ
                     AAVALEHGRAVMRGVLAQEIHHRVKNNLQTVASLLRLQARSPGIDARKALDDSVNRIL
                     AIAAVHEVLTERRDDDVELGELVERLRATLLQGVGGGKRVESRLEPVSLHGGRATALA
                     LVFSELLQNALEHGGDSVQVELARRNGEVRLSVADDGEWTGAAAEGTGLSIVRALVHD
                     ELGGTLTLGGEPGLRAEVRFPA"
     gene            4015..4494
                     /locus_tag="VFO88_00365"
     CDS             4015..4494
                     /locus_tag="VFO88_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544005.1"
                     /translation="MDLTQWLIALHVLAAFALVAALVLFSIVIAFGWNRDRPSEIVSL
                     FRVSRVGDVLFGVGAVGTILFGVWLAIKMDEYGIFDGWVIAALVLWAILMETARREGK
                     LYARSRDRARELFAGGADVSDAELRSLQRSQPALMFHVASCVLAFLLILDMIWKPGA"
     gene            4491..4970
                     /locus_tag="VFO88_00370"
     CDS             4491..4970
                     /locus_tag="VFO88_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544006.1"
                     /translation="MSLLAAIRPDDWNLPLFVHVFGAMLLVGGLGTALAFQVVGWRPE
                     RPDAAAGLARWSFRSLLFVAFPAWWIMRIGAQWIYTTEDWDSAAEEPTWIGIGWTTAE
                     GGGVFLLLGLILAGIGARRLSRSGARSSALVRISTVLVTVSLLMVVVATWAMSGKPA"
     gene            complement(5022..6389)
                     /gene="glnA"
                     /locus_tag="VFO88_00375"
     CDS             complement(5022..6389)
                     /gene="glnA"
                     /locus_tag="VFO88_00375"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET9544007.1"
                     /translation="MTMVEQEKDTGRATSDPQARKQVLDRVEQENVRYIHLWFTDLEG
                     HLKSFSITRQELADALDDGMGFDGSSITGFNPIEESDMVAIPDPKTFQLMPNGDVKVA
                     RMICDVVTPDGKPYDGDPRYVLRRAIERMKAMGFDTFNVGPELEYFLFEDDQGTKPLD
                     EGGYFAMTALDAAWELRNSTIDALESMGIPIEYVHHEVGPSQHEIDMRFANALDMADH
                     TITYRLIVKEVAKKAGYHATFMPKPLFGENGSGMHTHMSLFKEGRNAFFDADDEWSLS
                     EVGKAFIAGQLQHAREISAVFAQWVNSYKRLVPGYEAPVYVAWSRRNRSALIRIPLYK
                     PGKEQATRAEIRCPDPACNPYLTFAALLHAGLEGIEKGYELPPPMEQNLYNLTAEQRR
                     ERGIVALPETLGEAVDELATSELARRALGQHIFDAYVKIKREEWDEYRIQLTQWEMEK
                     YLAVL"
     gene            complement(6499..7002)
                     /locus_tag="VFO88_00380"
     CDS             complement(6499..7002)
                     /locus_tag="VFO88_00380"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9544008.1"
                     /translation="MADVLVLNASYEPLNVCTVRRAHVLVFKGKAEVVEELGKPLRSA
                     AETFVWPHVIRLLSYVRVPKSVQRKISRRALFARDGWRCQYCGTDSGKLTLDHVVPRS
                     RGGESSWENVVTSCAPCNLRKGNRMLEEVGMRLHAPPRAPAPALFVTLAAPRIPDRWH
                     PYLSAFA"
     gene            complement(7064..7477)
                     /locus_tag="VFO88_00385"
     CDS             complement(7064..7477)
                     /locus_tag="VFO88_00385"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9544009.1"
                     /translation="MTYRKAEIRPTRPEDEALLFGLARLDRGADERTLDVLEHETTFV
                     AEIDGRPAGYVALVVDADAGRVRIDQLFVAPEHEAEGVGRQLLEYAEGFAIAQGAGAL
                     DVVAAEDDARAVAFYRGRGFVPVGDDLFELVLPRT"
BASE COUNT          939 a         2796 c         2684 g         1116 t
ORIGIN      
        1 ttcccgggca cgctcctgct cgtctcgcac gaccgcgcgc tcctcgacgc gatcgccgag
       61 cgcacgctcg cgatcgagga cgggcgggtc cgctcgtacg agggcggctg ggccgactac
      121 gtccgcgcgc gcgccgagcg cgtggctccg ccggagccgc ccgtggagcc cgtccccgcg
      181 cgcaggccga ggacgccgaa gccggccgcc gcgaagaagg gccccgaccc gctcgtcgag
      241 ctcgagcgcg agatcgaggc gcaggagcgg gtcgtcgcgc gcctcgaggc cgagctcgcc
      301 gacgactgga cgaacatcga cacgatcgcg gcccaccgcc gcgcgcgcga ggatctccag
      361 cggctcttcg cgcgctggga ggagctcctc gaccgcgccc cttcgtgagg cgttccgcgg
      421 cccggacggc tggatcgacg ccgtccggct cgccgccgtg ccgttcgccg tcctcgaggc
      481 ggcgacggag ggctacgcgg agggtgagga gccgctcgcg tggctgctcg tcgcctcgct
      541 cgcgctcgcg gccgtcgcgc ttccgctcgc cgggcgctcc ggccgccctg cgtggctcgg
      601 cgccggcgcg ctcgtcttcg acaccgcgtt cctctcggcg tgggccgtcc tctacgcggc
      661 cgagccgggc acgcccgcgc gggagctcct cgtcctcgcc gccgtcgagg ccggcctgcg
      721 ctacgggccg cggggcgccc tctgggcgct cgcgacgctc cccgcgctcg tcctcttcga
      781 gctgcggctc tcgaacacgc tcgacgcccc gttcgacgtc ggccacgccg tcttcccggc
      841 cgggctctac gggctcgtcg ggctcgtcgt cggggcgctc agcgaggagc ggcggcgcgg
      901 aggaagtcca gcgccacccg ctgagccgcc ttcgtcggcg tgacgccgtc ggcctccgcc
      961 gcggcgagga cgcgctcgga cgcctcgcgg atgagccgct cgacctccgc caggcacgcg
     1021 gccgggtcgt cgccctcgca cccgggcgcc tcgtagaggt gcacgccgcc cgcgttcgcg
     1081 acgaagtcgg ggagcgcgag aatcccgcgc gcgtgcagcg cctccacggc gcccggcccg
     1141 tacgggatgt tggccgcggg cacgatcgcg gcgcagcgga gcagcggcgc ggtgtcgacg
     1201 gtgacgaagt cgggccgcgc acccggaacg agcacgtcgc actcgacggc ggcaagctcc
     1261 tcgcgcggcc gcaccgggac gccggcgtgg tggacgagcc ggtcgccgtg ctcggccttg
     1321 agggcgagca gcacgtcgac gtcgagcccc tccgggtcga gaagcgcgcc ctcgatcgtc
     1381 gagacgccca cgatccgggc gcccgcccgg gcgagggcgt gcgccgtccc cgcgccggcc
     1441 ttcccgaagc cttcgatcgc ggcgcgcgcg ccctcgagcg gccggccgag cgcggcgagc
     1501 gcggcccgcg ccgcgccgag gatcccggcc cccaccgcga ggtcgtccat tcccatcccg
     1561 tcgtgcgtcc gcgcccacat gggcgtgcgg ccctccggcg cgaggcggag gaagtcgctc
     1621 ggatgcgtcc ccatgtcagg cccggtgccg aagacgtcgc gcatcgcgag gatccggtcg
     1681 acgtaggccg agagcacgcg ctcgcgcgcg gccgcgtctc cgccggggaa ccggatcccc
     1741 gccttcgcgc ccgcgagcgg cacgtgggcg acgccgaact tccacgtcat cgcgcgggcg
     1801 agccgcgcca cctcgtccac cgtcacgtcg ggcaccatcc gcgtcccccc ggccgagcgg
     1861 gggaagagct cgtggtcgac cacgatgaag gcctcgagcc cgacggcgtc gtcgcggagc
     1921 tcgagaacgc ggagcggcgg cgcgttcggg tcgtcgagga cggacacggc gcgggacggt
     1981 actacgactc gtcggcgaag gtcgccacga tcgcctcggc gacgaccttc atcgggcggt
     2041 tggaggactg actcgcccgg cgcaggcgac cgaacgcctc ctcttcggag aggccctcgc
     2101 gctccatcag gagccccttc gcccgctcga ccaccttgcg cgtcgcgagc gcctcggcga
     2161 gcgactcggc ctcctcgcgg agcgcctcga gctccgcgtg gcgggcccgc gccgcgtgga
     2221 tcgccggcag gaggtcggcc tcgcggaacg gcttcacgag gtagccgaac acgccggcct
     2281 cgaccgcgcg ggcgacgagc tcctcctggc cgtacgcggt gagcatgacg atcgggatcg
     2341 gccgctcggc gaggatccgc cgcgccgcct cgatcccgtc gagccgcggc atcttcacgt
     2401 cgaggatcgc gaggtcgggc cgctcgctgg ccgcgagctc gaccgcctcc tcgccgtttc
     2461 gcgcctcggc gcagacctcg aacccggcct cctcgagcgt cgtcctgagg tcgagccgga
     2521 tgatcgtctc gtcctcggcg acgaggatcc tcacgccggg aaccgcacct cggcgcgcag
     2581 gccgggctcg ccgccgagcg tcagcgtgcc gccgagctcg tcgtgcacga gcgcgcggac
     2641 gatcgagagg cccgtgccct ccgccgcggc gccggtccac tcgccgtcgt ccgccacgct
     2701 cagccgcacc tcgccgtttc gccgcgcgag ctcgacctgc accgaatcgc ccccgtgctc
     2761 gagcgcgttc tggaggagct cgctgaagac gagcgcgagc gccgtcgccc gcccgccgtg
     2821 gagcgagacc ggctcgagcc gcgactccac gcgcttgccg ccgccgacgc cctgcaggag
     2881 cgtcgcgcgc agccgctcga cgagctcgcc gagctcgaca tcgtcgtcgc gccgctccgt
     2941 cagcacctcg tgaacggcgg cgatggcgag gatccggttc acggagtcgt cgagcgcctt
     3001 gcgggcgtcg atccccggcg agcgcgcctg caggcgcagg agcgaggcga ccgtctggag
     3061 gttgttcttc acgcggtgat ggatctcttg cgcgaggacg ccgcgcatga ccgcgcgacc
     3121 gtgctcgagc gcgaccgcgg cctggcttgc gatcgacgcg agcaggcggc gctcgtccgt
     3181 cgagaagggc gtgtcgcggt cgcagacgag ctcgccgatc tcgcggccct tccagcggag
     3241 cggcagccgc acggcgtgcg cgccggcgcg cccctcgggc caggcgatcc gcccgtcctc
     3301 gaggacgagc gcagcgcccg tcgcgccgac cgcggccatg gtcgtctcga cgatcgcgcc
     3361 gagcgactcc tcgaggtaga gcgactccga gaccgcctcg gagatcttcg cgagcgcctc
     3421 gagctccgcc acccggcgct gcgccgcgtc gtagagcttc gcgttctcga tcgcctgggc
     3481 gacctggccc gcgatcgcca tcaggagcgc gatctccgcg tcgctgaact cgcgcggctc
     3541 ctgcgtacgg acgttgaggg cgccctcgag gcggtcgcgc gcgaggatcg gcacggcgag
     3601 gatggactcg tactgctcct cgcggaggtt cgggaacgcc ttgaaccgct cgtcgaggtg
     3661 cgcggcgcgc gggatcatga ccggcgcgcg ctcggccgcc gcggtcccgg tgatcccctc
     3721 gccggggcgc atcttcggga cgcgcgtcat gtcctcgacc cgcgtgccgt acgacgcgcg
     3781 caggacgagc tcgtccgccg cctcgtcgta gaggtagacg aagcaggcgt cggcctcgag
     3841 cgcctccgcg accttcgcgg cgacgagctc gaagacctcg ccgaggtcga gcgtcgagtt
     3901 gaccgcggcg atcgtctcgg tgatgagccg caggtgccgc gccgacgcgt cgcgggcggt
     3961 ctcggacatg gacggattgt aggtcgccga cccgtccggt atcgtcgcgc gaccttggac
     4021 ctgacgcagt ggctcatcgc gctccacgtc ctcgccgcgt tcgcgctcgt cgcggcgctc
     4081 gtgctcttca gcatcgtgat cgcgttcggc tggaaccgcg accggccctc cgagatcgtc
     4141 tcgctcttcc gcgtctcgcg cgtgggcgac gtcctgttcg gcgtcggcgc cgtcgggacg
     4201 atcctcttcg gcgtctggct cgcgatcaag atggacgagt acgggatctt cgacggctgg
     4261 gtgatcgcag cgctcgtgct gtgggcgatc ctcatggaga ccgcgcggcg cgaagggaag
     4321 ctctacgccc ggtcgcgcga tcgggcgcgc gagctcttcg ccggcggcgc ggacgtctcc
     4381 gacgcagagc ttcgaagcct gcagcggtcg cagccggcgc tcatgttcca cgtcgcctcg
     4441 tgcgtgctcg cgttcctgct catcctcgac atgatctgga agccgggcgc atgagcctcc
     4501 tcgccgccat ccggccggac gactggaacc tcccgctctt cgtgcacgtc ttcggcgcca
     4561 tgctgctcgt gggcggcctc gggacggcac ttgcgttcca ggtggtcggc tggcgccccg
     4621 agcggcccga cgcggccgcc gggctcgcgc gctggagctt ccggtcgctc ctcttcgtcg
     4681 ccttccccgc gtggtggatc atgcggatcg gcgcccagtg gatctacacg accgaggact
     4741 gggacagcgc ggccgaggag ccgacctgga tcggcatcgg atggaccacc gcggagggcg
     4801 gcggcgtctt cctcctgctc gggctgatcc tcgccgggat cggcgcacgc cggctctcgc
     4861 gcagcggagc gcgttcgagc gcgctcgtcc ggatctcgac cgtgctcgtc acggtgtcgc
     4921 tcctcatggt cgtcgtggcc acgtgggcga tgtccggcaa gcccgcttaa cgcggcgggc
     4981 cgcccgaagg cggcccgccg ctgcgcgcag atgtgccggc gctagaggac ggccaggtac
     5041 ttctccatct cccactgcgt gagctggatg cggtactcgt cccactcctc gcgcttgatc
     5101 ttcacgtaag cgtcgaagat gtgctggccg agcgcccgcc gagcgagctc cgaggtggcg
     5161 agctcgtcga ccgcctcgcc gagcgtctcc gggagggcga cgatcccgcg ctcgcgccgc
     5221 tgctccgccg tgaggttgta gaggttctgc tccatcggag gcgggagctc gtagcccttc
     5281 tcgatcccct cgagccccgc gtggaggagc gcggcgaacg tgaggtacgg gttgcaggcc
     5341 gggtcggggc agcggatctc cgcccgcgtc gcctgctcct tgcccggctt gtagagcggg
     5401 atccggatga gcgccgagcg gttccgccgc gaccacgcga cgtagaccgg cgcctcgtag
     5461 cccggcacga gccgcttgta cgagttcacc cactgcgcga agaccgcgga gatctcgcgc
     5521 gcgtgctgga gctgccccgc gatgaacgcc ttgccgacct ccgagaggct ccactcgtcg
     5581 tccgcgtcga agaacgcgtt ccggccttcc ttgaagagcg acatgtgcgt gtgcatcccc
     5641 gagccgttct cgccgaagag cggcttcggc atgaacgtcg cgtggtaccc ggccttcttc
     5701 gcgacctcct tcacgatcag gcggtacgtg atcgtgtggt cggccatgtc gagcgcgttc
     5761 gcgaagcgca tgtcgatctc gtgctgcgag ggaccgacct catggtggac gtactcgatc
     5821 gggatcccca tggactcgag cgcgtcgatc gtcgagttgc ggagctccca ggccgcgtcg
     5881 agcgcggtca tggcgaagta cccgccctcg tcgagcggct tcgtgccctg gtcgtcctcg
     5941 aagaggaagt actcgagctc cggcccgacg ttgaacgtgt cgaagcccat cgccttcatc
     6001 cgctcgatcg cgcgccgcag gacgtagcgc gggtcgccgt cgtacggctt cccgtccggc
     6061 gtcacgacgt cgcagatcat ccgcgcgacc ttcacgtcgc cgttcggcat gagctggaac
     6121 gtcttcgggt ccgggatcgc gaccatgtcg gactcctcga tcgggttgaa cccggtgatc
     6181 gaggagccgt cgaagcccat cccgtcgtcg agcgcgtccg cgagctcctg cctcgtgatc
     6241 gagaagctct tcaggtgccc ctcgaggtcg gtgaaccaga ggtggatgta gcggacgttc
     6301 tcctgctcga cccggtcgag gacctgcttg cgcgcctgcg ggtcgctggt cgccctgccg
     6361 gtgtccttct cctgctccac catcgtcatc tacgcgcctc cttaaatgga aagagccccg
     6421 gacccgtcgc gggcccgagg cttcgtcgcc tcacagcggc tctcagggta gcgcgtttcg
     6481 cggacggcgt cgaatcggct acgcgaacgc cgagaggtac gggtgccagc ggtccgggat
     6541 gcgcggcgcg gcgagcgtga cgaagagcgc cggggccggc gcgcgcggcg gtgcgtggag
     6601 gcgcatgccg acctcctcga gcatccggtt ccccttgcgg aggttgcacg gcgcgcacga
     6661 ggtgacgacg ttctcccagc tcgactcgcc ccctcggctg cgcggcacga cgtggtcgag
     6721 cgtgagcttt cccgagtccg tcccgcagta ctggcagcgc cagccgtcgc gggcgaagag
     6781 cgcccggcgc gagatcttcc gctgcaccga ctttggcacg cggacgtagc tcaggagcct
     6841 gatcacgtgc ggccagacga aggtctcggc cgcggagcgg agcggcttgc cgagctcctc
     6901 gaccacctcg gccttgccct tgaacacgag gacgtgggcc cggcgaaccg tgcacacgtt
     6961 gagcggctcg taactcgcgt tcaggacgag cacgtcggcc ataccgcgcg aaatcgtagc
     7021 gtggagacac gccccgacga acccggttcc gcctggtggc ggatcaggtg cgcggaagga
     7081 cgagctcgaa gaggtcgtcg cccaccggca cgaagccgcg gccgcggtag aacgcgacgg
     7141 cccgggcgtc gtcctcggcg gcgacgacgt cgagcgctcc ggcgccctgg gcgatcgcga
     7201 agccctccgc gtactcgagg agctggcggc cgaccccttc cgcctcgtgc tcgggcgcga
     7261 cgaacagctg gtcgatgcgc acgcgccccg cgtcggcgtc cacgacgagc gcgacgtagc
     7321 cggcgggccg gccgtcgatc tcggcgacga aggtcgtctc gtgctcgagg acgtcgagcg
     7381 tccgctcgtc cgcgccgcgg tcgaggcggg cgagcccgaa gaggagcgcc tcgtcctcgg
     7441 gtcgcgtcgg gcggatctcc gccttccggt acgtcacgct ctggccctac ccggtcgcca
     7501 tcgcacggtc gaccgcctcc tgctcttccg gggag
//
LOCUS       DASTFC010000014         3750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_923117,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000014 DASTFC010000000
VERSION     DASTFC010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923117"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFO88_00390"
     CDS             complement(<1..359)
                     /locus_tag="VFO88_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9544010.1"
                     /translation="MSNRALVLVAAVAVLWGIPYLFIAVALEELSPAVIAFARTGLAA
                     AILLPLALAGGALAGLRERWAWLVVLAATQMAGPFLLLGFAEERISSSLTGILVSATP
                     LLVALVALGVDRSESASG"
     gene            472..1218
                     /locus_tag="VFO88_00395"
     CDS             472..1218
                     /locus_tag="VFO88_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9544011.1"
                     /translation="MRLHLHEWGDRALPTILCLHGVKAHGARFRRLAEERLTDRFHVV
                     APDLRGHGRSPWDPPWSLPAHVDDVEELADDLSPAIVLGHSFGGRLVLELAARSPRRV
                     PRAILLDPAVQVPRDVAVAYAAEEAARTPFASRDAALESRAAEARRAPRELLEEELDA
                     HLTDELRFRYSPLAAATIFGELAGDPPASPRCPTLLVVAETTDITTPEAVARLEDTVD
                     ELTVARVPGGHIVLWDAFAETAGAIEAFLA"
     gene            complement(1215..2114)
                     /gene="rocF"
                     /locus_tag="VFO88_00400"
     CDS             complement(1215..2114)
                     /gene="rocF"
                     /locus_tag="VFO88_00400"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HET9544012.1"
                     /translation="MPAGRVAIIGAPLDLGAGRRGVDMGPSAIRYAGLGERLAGLGLV
                     VEDRGNVGAALPETISTGNERARFLPEIKATCERIAAEVSRAADEGLMPLVLGGDHSI
                     ALGTVGGMAAARGVGAAIWLDAHGDLNTPETTPSGNVHGMPLAAALGAAEDGFGSEAW
                     PLPALDPARTSLVGVRSLDEGERALVRELGLEVFTMSELDRRGVQAVLADALERARGA
                     AFVHVSLDMDAVDPDVAPGVGTPVRGGLSYREAHLAMELVAESGLLDSFEIVEVNPIL
                     DVSNETAALAVELAASALGASIL"
     gene            2152..2224
                     /locus_tag="VFO88_00405"
     tRNA            2152..2224
                     /locus_tag="VFO88_00405"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2185..2187,aa:Phe,seq:gaa)
     gene            complement(2271..2897)
                     /locus_tag="VFO88_00410"
     CDS             complement(2271..2897)
                     /locus_tag="VFO88_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9544013.1"
                     /translation="MSERLVAVGRRFARLSTRAVVGRPWAWRLFRAPLRRYFDWLAPQ
                     WESIRGPEGLLPLAAALDRLDAPPRRVLDVGTGTGAALALVAGRFPDAELVGVDIAPS
                     MLAEARRVLSADVLARARLEVADAAALPFAEREFDLVTLQNAIPFPAELGRVVAPGGT
                     VVVAFSRGPETPIWTPPETLKRLLAEAGFERFEEVTAGAGTALLARRS"
     gene            complement(2894..3703)
                     /locus_tag="VFO88_00415"
     CDS             complement(2894..3703)
                     /locus_tag="VFO88_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET9544014.1"
                     /translation="MAHRFITFLTDFGLEDDFVGTCKGAMKRIAPDVEIIDVTHGIEP
                     QSILQGALVLAGTLPYMPAGVHLAVVDPKVGSERKALALRGPDHVFVGPDNGLLLPAA
                     ERHGGIEEAVSIENPAYRLERVSKTFHGRDVFAPAAAHLALGVPLAELGPSVDPAGLE
                     RLDIPRPEVGDGKLRVKVLYVDRYGNVQVSATADDLAAAGMEEGTRVELDLGFESYFA
                     TVAGTFADVRDGDILLYEDSYWNVALAINGGNAAETLAVRPGADLRVRRAT"
BASE COUNT          509 a         1424 c         1377 g          440 t
ORIGIN      
        1 cccgatgcgc tctccgagcg gtcgaccccg agcgcgacga gcgcgacgag gagcggggtc
       61 gccgacacga ggatcccggt gagcgacgag gagatccgct cctccgcgaa gccgagcagg
      121 aggaacggcc cggccatctg cgtcgccgcg aggacgacga gccaggccca gcgctcccgc
      181 agcccggcga gcgcgccccc ggcgagcgcg agcggcagga ggatcgccgc cgcgagcccg
      241 gtgcgggcga acgcgatcac cgccggcgag agctcctcca gcgcgaccgc gatgaagagg
      301 taggggatgc cccagaggac ggcgacggcg gcgacgagga cgagggcgcg gttgctcatg
      361 gacgttgaaa cggcaaacga ccgccgcgtg tgagatctgc ccggcgagcg gctgtccccc
      421 gcggccacgg ccggtgcctg gcaccggaca cggccgttag ggtcgtccgc gatgcggctg
      481 cacctgcacg agtggggcga ccgggcgctc cccacgatcc tctgcctcca cggcgtgaag
      541 gcgcacggcg cgcggtttcg gcggctcgcc gaggagcggc tgaccgaccg cttccacgtc
      601 gtcgcccccg acctgcgcgg gcacgggcgc tcgccgtggg atccgccgtg gagcctcccg
      661 gcccacgtgg acgacgtcga ggagctcgcg gacgacctct cgcccgcgat cgtgctcgga
      721 cacagcttcg gcgggcggct cgtgctcgag ctcgccgcgc gaagcccgag gcgcgtgccg
      781 cgcgcgatcc tgctcgaccc ggccgtgcag gtgccgcgcg acgtggccgt cgcgtacgcc
      841 gccgaggagg cggcacggac gccgttcgca agccgcgacg cggcgctcga gagccgcgcg
      901 gccgaggccc ggcgggcgcc ccgcgagctc ctcgaggagg agctggacgc gcacctgacg
      961 gacgagctgc gcttccgcta ctcgccgctc gcggccgcga cgatcttcgg cgagctcgcc
     1021 ggcgacccgc ccgcctcgcc gcgctgtccc accctgctcg tcgtggcgga gacgaccgac
     1081 atcacgacgc ccgaggcggt cgcgcgcctc gaggacaccg tggacgagct gacggtcgca
     1141 cgcgtccccg gcggccacat cgtcctctgg gacgccttcg ccgagacggc gggcgcgatc
     1201 gaggcgttcc tcgcctaaag gatgctggct ccgagcgcgc tcgcggccag ctcgaccgcc
     1261 agcgcggcgg tctcgttcga cacgtcgagg atcgggttca cctcgacgat ctcgaacgag
     1321 tcgaggaggc cggactcggc gacgagctcc atcgcgaggt gcgcctcgcg gtacgagagg
     1381 cccccgcgca ccggcgtccc gacgccgggc gccacgtcgg ggtcgacggc gtccatgtcg
     1441 agcgagacgt ggacgaaggc agcgccccgc gcccgctcga gcgcgtcggc cagcaccgcc
     1501 tgcacgcccc ggcggtcgag ctcgctcatc gtgaagacct cgagcccgag ctcacgcacg
     1561 agggcgcgct cgccctcgtc gagcgagcgg acgccgacca gcgacgtcct cgccgggtcg
     1621 agcgcgggca gcggccacgc ctcgctcccg aagccgtcct cggccgcgcc gagcgccgcg
     1681 gcgagcggca tgccgtgaac gttccccgac ggcgtcgtct ccggtgtgtt caggtcgccg
     1741 tgcgcgtcga gccagatggc cgcgcccacg ccccgcgcgg cggccatccc gccgaccgtg
     1801 ccgagcgcga tcgaatggtc gcctccgagc acgagcggca tgagcccctc gtcggcggcg
     1861 cgtgacacct ccgcggcgat ccgctcgcag gtggccttga tctccgggag gaagcgggcg
     1921 cgctcgttgc cggtggagat cgtctccggg agcgccgcgc cgacgttccc gcggtcctcc
     1981 acgacgagcc cgagccccgc gagccgctct ccgagccccg catagcggat cgccgacggg
     2041 cccatgtcca cgccgcgccg gcccgctccg aggtcgagcg gggccccgat gatcgccacg
     2101 cgtcccgcag gcatccggcc gaatctactt ggctacactg cgcactcgcg cggcgaggta
     2161 gctcagttgg tagagcacac ggctgaaaac cgtggtgtcg ccggttcgat tccggccctc
     2221 gccattcgcc cctgcgagtc cgcccgcccg gtcgggcgcg gcgacgaacc tcaggatcgg
     2281 cgggcgagga gcgccgtgcc ggcgccggcg gtgacctcct cgaagcgctc gaagccggcc
     2341 tcggcgagga ggcgcttcag cgtctccggc ggtgtccaga tcggcgtttc cgggccgcgc
     2401 gagaacgcga cgaccacggt gccgcccggc gcgacgacgc gcccgagctc ggccgggaac
     2461 gggatcgcgt tctggagcgt cacgaggtcg aactcgcgct ccgcgaaggg caaagccgcg
     2521 gcgtccgcga cctcgagcct ggcgcgcgcg agcacgtccg ccgacagcac ccgccgcgcc
     2581 tcggcgagca tcgacggcgc gatgtcgacg ccgacgagct ccgcgtccgg gaaccgcccg
     2641 gcgacgagcg cgagcgccgc gccggtcccg gtgccgacgt cgagcacgcg gcgcggcggc
     2701 gcgtcgagcc gatcgagcgc ggccgcgagc gggagcaggc cctccggccc gcggatgctc
     2761 tcccactgcg gcgcgagcca gtcgaagtag cggcggagcg gcgcccggaa gagccgccag
     2821 gcccacggcc tgcccacgac cgcccgcgtc gacagacggg cgaagcgccg cccgaccgcg
     2881 acgagccgct cgctcacgtc gcgcggcgaa cccggaggtc ggctcccggc cgcacggcga
     2941 gggtctcggc cgcgttcccc ccgttgatcg cgagcgcgac gttccagtac gagtcctcgt
     3001 agaggaggat gtcgccgtcc cgcacgtctg cgaacgtccc cgcgacggtc gcgaagtacg
     3061 actcgaagcc caggtcgagc tcgacgcgcg tgccctcctc catcccggcc gcggcgaggt
     3121 cgtcggccgt cgcgctcacc tggacgttcc cgtagcggtc gacgtagagc accttcacgc
     3181 ggagcttgcc gtcgccgacc tcgggacgcg ggatgtcgag ccgctcgagg cccgccgggt
     3241 cgacggacgg gccgagctcc gcgagcggga cgccgagcgc gaggtgcgcg gccgccgggg
     3301 cgaagacgtc gcggccgtgg aacgtcttcg aaacgcgctc gagccggtag gcggggttct
     3361 cgatcgagac cgcctcctcg atcccgccgt gccgctcggc ggccggcagg agcagcccgt
     3421 tgtccgggcc gacgaagacg tggtcgggcc cgcggagcgc gagcgccttc cgctcgctcc
     3481 ccaccttcgg gtcgacgacc gcgaggtgca cgccggccgg catgtacggg agcgtgcccg
     3541 cgagcacgag cgccccctgg aggatgctct gcggctcgat cccgtgcgtg acgtcgatga
     3601 tctcgacgtc cggcgcgatc cgcttcatcg cccccttgca ggttccgacg aagtcgtcct
     3661 cgagcccgaa gtcggtcagg aacgtgatga accggtgcgc gatgcggcga gcttaacggc
     3721 ttcggccagc ccgtcgagat cgtgggtctc
//
LOCUS       DASTFC010000015         8473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_741857,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000015 DASTFC010000000
VERSION     DASTFC010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741857"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFO88_00420"
     CDS             complement(<1..1067)
                     /locus_tag="VFO88_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9544015.1"
                     /translation="MVTPRRTALALAALVGVLLATPGAHGQSLSERQQTLDGKISSLR
                     SKIQSAKEKEGVLSSEIQAASTDISSLENDIGVLTGTLSTLEAELAASRARLANLEAR
                     FDRQTDHLNRLRRDFALAQQTLDERLVELYTAGETDTVALILGVTSLSELIAQLDFVN
                     EISLQDQRIAADLERLKGEMRLARIETGKLRDQVADETATIAAKTEEARAAQAALVAR
                     QSALEAARADKQSLLAGVKEDRHHAEEDLDALQAASAQLAAQIQAAQAASSGGSGGSG
                     GSSGSGASPSSGFIWPTSGVVTSGFGWRWGRMHEGIDIAAPTGTSVQAVAAGTIIYAG
                     VMGGYGNIVVIDHGNGLSTAYA"
     gene            complement(1186..1899)
                     /locus_tag="VFO88_00425"
     CDS             complement(1186..1899)
                     /locus_tag="VFO88_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET9544016.1"
                     /translation="MRDCPNCGTSVPASARFCHECGRPLEEGVAPGKRFRITPALVGA
                     MALAAVSAALFVAGLWAWGLVALLGALVVLLVASPLGGGSARTLIAEARTRASVTGEA
                     VAARSRGHVELFRARRELADLEAERGRRLLELGCAVYDGDEAGTRTAREAVDETVSRI
                     REKEAEIETARKEVDERVERAQSQARPTVVMETPPEPPSIPEPWPPPDEADRPTPEPD
                     PGPATPEPEPGEASPPLGR"
     gene            complement(1892..2239)
                     /locus_tag="VFO88_00430"
     CDS             complement(1892..2239)
                     /locus_tag="VFO88_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544017.1"
                     /translation="MEVLALALGAALALACVVYAALPFLREPAAEDDTLGDLTPARRR
                     RLALEEERDRALAALKELEFDHRTGKVSDEDYRAQVVVLRREAAEALRALDAPAPATD
                     PDAAPGEKVDARA"
     gene            complement(2239..4233)
                     /locus_tag="VFO88_00435"
     CDS             complement(2239..4233)
                     /locus_tag="VFO88_00435"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET9544018.1"
                     /translation="MPLLGRAALIVALGLLVYAAVAGSVAAYRGRRRLLESARNALVG
                     ALAATVVASLVLVAALAGRDFRFAYVADLTSRELPLPYALSAFWGGQEGSLLLWLLVL
                     LGMSVAAVLLNKRLTRDVLPWTVPILAGVAVFFAVLLVFVASPFATRAVVADGFGLNP
                     SLQNPYMLSHPPLLYLGYVGLTIPFAFAMAALASKRVDERWIVATRRWTLGAWTFLGV
                     AILLGAKWAYEEVGWGGWYAWDPVENAALMPWLVATAFLHSVMVQEKKDMLRVWNVLL
                     VAGAFALSLFGTFLTRSGILSSIHSFTQSSIGPWFLGFITVVLAGSIALIIWRLPILR
                     SRTRLESLVSREAAFLYNNLFLVAFALTILWGVLYPILSEAVRGVAVTVGAPYYDFFA
                     IAFGLPIVLLMGLGPLVAWRRASLRALGRSVAWPAGVALVVGAVLVALGAGSSPAGLV
                     GYTFAAFVLAAIVLEFARGTRARKAAGSATWLGAFTSLVGRNRRRYGGYVVHAAIVLL
                     LVGAVGIGGFSTSSDARLSPGQSVEVGAYTLTYLGATERRAANAVELRAELAVERGGE
                     PIGTIEPGKNRYLAEGQTSNEVGIRTDLLRAEDLFVIADSFEGEAVFLEVFVNPLVNL
                     IWLAGFVFLAGAVVTMWPDAREARRLARRFADGEAALARA"
     gene            complement(4245..4640)
                     /locus_tag="VFO88_00440"
     CDS             complement(4245..4640)
                     /locus_tag="VFO88_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET9544019.1"
                     /translation="MARRRNPARLVIALSVAAVLAVFLVYTSLAGGGTPMVQPSELAA
                     QTGAVQLGGIVVGPVEGDARGDGLRFELRDIKGQETVPVVYTGSVPDMFREGREIMVR
                     GELANGVFTAERDSLVTKCPSKYSPADDA"
     gene            complement(4672..4911)
                     /locus_tag="VFO88_00445"
     CDS             complement(4672..4911)
                     /locus_tag="VFO88_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544020.1"
                     /translation="MTKRPSRIELLELDIDVRLADLWAEASDVGEWNLEVVAAFMRAA
                     YGKGYCDALTEDDPGSLCRDHGYRIPLKHPSPLPE"
     gene            complement(4953..5474)
                     /locus_tag="VFO88_00450"
     CDS             complement(4953..5474)
                     /locus_tag="VFO88_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET9544021.1"
                     /translation="MRLAAAIALVLVVVAVAGCQRDTDLAGERATVAEVIDGDTLRLD
                     DGRVVRLVQIDAPEPRDRECYGADATAALLELAPEGTEVTLVRDGVLDSVDRFDRLLR
                     YVFVGARNLNLELVREGAAAPYFFDGDRGRYADRLLDAAEKARDEGVGLWGACPEAEL
                     VPERELDAGPERL"
     gene            complement(5471..6103)
                     /locus_tag="VFO88_00455"
     CDS             complement(5471..6103)
                     /locus_tag="VFO88_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9544022.1"
                     /translation="MRVFVTGGSGVLGRALLPLLAAASHPVDAPARDELDLFDAEAVR
                     EAVAPADAVVHLATRIPPPERRADPSAWRENDRLRAEASRILVDAALAGRAAVYVQPA
                     VTFVYPKDGAVDEETPVRDVAPHLRSALAAEAEAARFAAAGRRGVVLRLGLLGGRGAD
                     SHFGTLDPGDAGAALLAALDAPSGTYNAVRDGGRVSNRRLRETTGWRPRR"
     gene            6128..6952
                     /locus_tag="VFO88_00460"
     CDS             6128..6952
                     /locus_tag="VFO88_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9544023.1"
                     /translation="MGAIRFGPSRLPSRESPEAAVELLVERGFTACEVDFEGGFWMKP
                     EWEWAARLGEAAREAGIVLSVHAPIAAFPGHLSGDEGKRKRAFGMLDHTAGVAVALGA
                     EVVVFHPGFLLGRSREEALDAVVSDLAALRERLEGKERGVPFGIEIMGRVRELGTAED
                     VFTVAARLPWVRPVLDFAHLHAVTDGGFRAADDFAAILGPADELLEPGAPFHIHFSDI
                     AFANRNETKHLPYGEGTLRAEPLAEALARFDRPATVISESPDLASDQAIGAVLAAA"
     gene            complement(6949..7740)
                     /locus_tag="VFO88_00465"
     CDS             complement(6949..7740)
                     /locus_tag="VFO88_00465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9544024.1"
                     /translation="MAGSDTFRAAAAYDRAVGRYGPSLAAGLIRFAGVEPGMRALDVG
                     CGTGLLTAALAERLGAEKVTGAEPSEPFAEAASSRVPGAEIAVAAAEDLPFPDDAFDA
                     ALSQLVLNFMRDAEAGVRELARVTRPDGVVASCVWDYAGEMTLLRAFWDAAREVEPER
                     GAAADEGVVMRWCSEGELLRLWESAGLREVRTGALTARAAYESFDDLWEPFPSGIAPS
                     GAFTASLDEDGRAALREAFRRRLGVGDEPFELEARAWAVRGVVPG"
     gene            7785..8051
                     /locus_tag="VFO88_00470"
     CDS             7785..8051
                     /locus_tag="VFO88_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544025.1"
                     /translation="MTEPAEHPAERDETGMRGRRASFLPPRGERWGLESLFVRVVATG
                     GIIGIGVALAAILDWQDVAAWIIGIVVSGVSVLLAALLWSSRTL"
     gene            8063..>8473
                     /locus_tag="VFO88_00475"
     CDS             8063..>8473
                     /locus_tag="VFO88_00475"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET9544026.1"
                     /translation="MGHGARPPEELVACLMEAGVETLVDVRRFPSSRRHPHFNRDPLA
                     ATLEEAGIAYRHAEELGGRRGDQPGEERFACIQVAAFRSYVARMTTPEWQEALEAELA
                     QPAPCFMCAETPWWRCHRRFIAELLTARGHEILHL"
BASE COUNT         1157 a         3158 c         3138 g         1020 t
ORIGIN      
        1 gcgtacgcgg tcgagaggcc gttcccgtgg tcgatcacga cgatgttgcc gtagccgccc
       61 atcacgccgg cgtagatgat cgtgccggcc gcgaccgcct gaaccgaagt gcccgtcggg
      121 gcggcgatgt cgatcccctc gtgcatgcgg ccccagcgcc agccgaagcc gctcgtgacg
      181 acgccgctcg tcggccagat gaagccgctc gacggcgagg cgccgctccc gctcgagccg
      241 ccggagccgc ccgagccgcc agacgaagcc gcctgcgcgg cctggatctg tgcggcgagc
      301 tgggcgctcg cggcctggag cgcgtcgagg tcctcctccg cgtggtggcg gtcctccttg
      361 acgcccgcga gcagcgactg cttgtccgcg cgcgccgcct cgagggcgct ctgccgcgcg
      421 acgagggcgg cctgcgccgc gcgggcctct tccgtcttcg ccgcgatcgt ggccgtctcg
      481 tcggcgacct ggtcgcggag cttgcccgtc tcgatgcgcg cgagccgcat ctcgcccttc
      541 agccgctcga ggtcggcggc gatccgctgg tcctgcaggc tgatctcgtt cacgaagtcg
      601 agctgcgcga tcagctcgct gaggctcgtg acgccgagga tgagcgcgac cgtgtccgtc
      661 tcgcccgcgg tgtagagctc gacgagccgc tcgtcgagcg tctgctgggc gagcgcgaag
      721 tccctgcgca gccggttcag atggtcggtc tgccggtcga agcgcgcctc gaggttcgcg
      781 agccgggcgc gggaggccgc gagctccgcc tcgagcgtcg acagggtccc cgtgagcacc
      841 ccgatgtcgt tctcgagcga ggagatgtcg gtggacgcgg cctggatctc cgacgagagg
      901 acgccctcct tctccttggc ggactggatc ttgctgcgca gcgaagagat cttcccgtcc
      961 agggtctgct ggcgctcgga cagcgactgg ccgtgcgctc ccggggttgc gagcaggacg
     1021 ccgacgaggg ctgccagtgc gagggctgtc ctgcgggggg taaccacgat acgaggattc
     1081 gcggagcggg caggcgtccc tgcgccgccg agcatgtgtt ctcgttccgg ggctttctac
     1141 acgaaacgcc ggacaggggc aacgagagcc gcccccgacc ggggctcagc gtccgagcgg
     1201 cgggctcgcc tcgcccggct ccggctcggg cgtcgcaggg cccggatcgg gctcgggcgt
     1261 cggccggtcg gcctcgtccg gcggcggcca cggctcgggg atgctcggcg gctccggcgg
     1321 cgtctccatc acgaccgtcg ggcgggcctg cgactgggcg cgctcgacgc gctcgtcgac
     1381 ctctttgcgt gcggtctcga tctcggcctc cttctcgcgg atcctggaga ccgtctcgtc
     1441 gaccgcctcc cgtgccgtcc gcgtgcccgc ctcgtcgccg tcgtacaccg cgcatccgag
     1501 ctcgagcagc cggcggccgc gctcggcctc gagatcggcg agctcccggc gggcgcggaa
     1561 gagctcgacg tgcccgcgcg agcgcgccgc gaccgcctcg cccgtcacgc tcgcgcgcgt
     1621 ccgcgcctcg gcgatcagcg tgcgcgccga gccgcccccg agcgggctcg cgaccaggag
     1681 gacgacgagt gcgccgagaa gcgccacgag cccccacgcc cagaggcccg ccacgaagag
     1741 cgccgccgag accgccgcga gcgccatggc gcccacgagc gccggcgtga tccgaaagcg
     1801 cttgcccggc gccacgccct cctcgagcgg gcggccgcac tcgtggcaga accgcgccga
     1861 cgccggaacc gaggtcccgc agttcgggca gtcacgcacg ggcgtcgacc ttctccccgg
     1921 gcgccgcgtc gggatccgtc gcgggtgcgg gcgcgtcgag cgcgcgcagc gcctcggccg
     1981 cctcgcggcg gaggacgacc acctgcgccc ggtagtcctc gtccgagacc ttccccgtcc
     2041 ggtggtcgaa ctcgagctcc ttgagcgcgg cgagcgcgcg gtcgcgctcc tcctcgagcg
     2101 cgaggcggcg ccgccgggcc ggggtcaggt cgccgagcgt gtcgtcctcc gcggccggct
     2161 cccgcaggaa cggaagggcg gcgtagacga cgcaggcgag cgcgagcgcg gccccgagcg
     2221 cgagcgcgag gacctccatc aggcgcgcgc gagcgccgcc tccccgtcgg cgaaccgccg
     2281 ggcgagccgg cgggcctcgc gcgcgtccgg ccacatcgtc acgaccgctc ccgcgaggaa
     2341 gacgaagccc gcgagccaga tgaggttcac gagcgggttc acgaacacct cgaggaagac
     2401 cgcctcgcct tcgaacgagt cggcgatcac gaagaggtcc tccgcgcgga ggaggtcggt
     2461 gcggatgccg acctcgttcg aggtctgacc ctcggcgaga tagcggttct tcccgggctc
     2521 gatcgtcccg atcggctctc ccccgcgctc gaccgcgagc tcggcgcgga gctcgaccgc
     2581 gttcgccgcc cgccgctcgg tcgcgccgag gtacgtgagc gtgtaggcgc cgacctcgac
     2641 cgactgcccg ggcgagaggc gcgcgtccga cgaggtcgag aaccctccga tcccgaccgc
     2701 gccgacgagg aggagcacga tcgccgcgtg gaccacgtag ccgccgtagc ggcgccggtt
     2761 ccgccccacg aggctcgtga acgcgccgag ccacgtcgcc gagccggccg ccttccgggc
     2821 ccgcgtcccg cgcgcgaact cgagcacgat cgcggcgagc acgaaggcgg cgaacgtgta
     2881 gccgacgagc cccgccgggc tcgagccggc gccgagcgcc acgagcaccg cgccgacgac
     2941 gagcgcgacc cccgccggcc acgcaaccga gcggccgagc gccctgagcg acgcgcggcg
     3001 ccaggcgacg agcggcccga gccccatgag gagcacgatc ggcagcccga acgcgatcgc
     3061 gaagaagtcg tagtacggcg cgccgaccgt gaccgcgacg ccgcgcaccg cctccgagag
     3121 gatcggatag aggacgcccc agaggatcgt gagcgcgaac gcgacgagga agaggttgtt
     3181 gtagaggaac gcggcctccc gggagacgag cgactcgagc cgcgtccgcg agcgcaggat
     3241 cggcaggcgc cagatgatga gcgcgatcga ccccgcgagc acgaccgtga tgaagccgag
     3301 gaaccacggc ccgatcgagc tctgcgtgaa ggagtggatc gagctcagga tcccgctgcg
     3361 cgtgaggaaa gtcccgaaga gcgagagggc gaacgcgccg gccacgagga gcacgttcca
     3421 gacgcgcagc atgtccttct tctcctgcac catcaccgag tggaggaacg cggtggcgac
     3481 gagccacggc atgagcgccg cgttctcgac cggatcccag gcgtaccagc cgccccagcc
     3541 gacctcctcg taggcccact tcgcgccgag caggatcgcc acgccgagga acgtccaggc
     3601 gccgagcgtc cagcggcgcg tcgcgacgat ccaccgctcg tccacgcgct tcgaggcgag
     3661 cgcggccatc gcgaacgcga acgggatggt gaggccgacg tagccgaggt agaggagcgg
     3721 cgggtgcgag agcatgtacg ggttctggag gctcgggttc aggccgaagc cgtccgcgac
     3781 cacggcgcgg gtcgcgaacg ggctcgcgac gaagacgagc agcacggcga agaagacggc
     3841 gaccccggcg aggatcggca ccgtccacgg cagcacgtcc cgcgtcagcc gcttgttcag
     3901 gaggaccgcg gcgacgctca tcccgagcag gacgaggagc cagaggagga gcgagccctc
     3961 ctgcccgccc cagaacgcgg agagcgcgta cggaagcgga agctcgcggc tcgtcaggtc
     4021 ggcgacgtag gcgaagcgga agtcgcggcc ggcgagcgcg gcgacgagga cgagcgacgc
     4081 gacgacggtc gccgcgagcg caccgacgag cgcgtttcgc gccgactcga gcagccgccg
     4141 gcggccccgg taggccgcga cggagcccgc cacggccgcg tagacgagga gcccgagagc
     4201 gacgatcagg gcggcccggc cgagcagcgg cacggctcag ccctctacgc gtcgtccgcg
     4261 ggcgagtact tcgaggggca cttcgtgacg agcgagtccc gctcggccgt gaagaccccg
     4321 ttcgcgagct ccccgcggac catgatctcg cgcccctcgc ggaacatgtc gggcacggag
     4381 cccgtgtaga cgaccggcac ggtctcctgc cccttgatgt cgcggagctc gaagcgcagc
     4441 ccgtcgccgc gggcgtcgcc ctccacgggg ccgacgacga tcccgccgag ctggacggcg
     4501 cccgtctgcg ccgcgagctc gctcggctgc accatcggcg tcccgccgcc cgcgaggctc
     4561 gtgtagacga gaaagacggc gagcaccgcc gcgaccgaga gcgcgatcac gagccgcgca
     4621 gggttccgcc gcctggccat cgcttcgagt ctaccggcgc ggcttcgcgc gctactcggg
     4681 caaggggctc gggtgcttca gcgggatccg gtagccgtgg tcgcggcaga ggctccccgg
     4741 atcgtcctcc gtcagggcgt cgcagtagcc cttcccgtag gcggcccgca tgaaggccgc
     4801 gaccacctcg aggttccact cgccgacgtc ggacgcctcc gcccagaggt cggcgagccg
     4861 gacgtcgatg tccagctcga gcaactcgat ccgcgagggc cgcttcgtca tggcgaaaac
     4921 ggtacgagcc ccgtcggacg gcattccggc ggtcagagcc gttccgggcc cgcgtcgagc
     4981 tcgcgctcgg gaacgagctc ggcctccggg caggctcccc acaggccgac gccctcgtcg
     5041 cgggccttct cggccgcgtc gagcagtcgg tcggcgtagc ggccccggtc gccgtcgaag
     5101 aagtacggcg cggccgcgcc ttcccgtacg agctcgaggt tgaggttccg tgccccgacg
     5161 aagacgtagc ggagcagccg gtcgaaccgg tcgacgctgt cgaggacgcc gtcgcgcacg
     5221 agcgtgacct cggtgccctc cggcgcgagc tcgaggagcg ccgccgtcgc gtcggcgccg
     5281 tagcactcgc ggtcgcgcgg ctccggcgcg tcgatctgga cgagccggac gacccggccg
     5341 tcgtccaggc gcagcgtgtc cccgtcgatc acctcggcga ccgtcgcacg ctcgccggcg
     5401 aggtcggtgt cacgctggca acccgcgacg gccacgacga cgagcacgag ggcgatggcg
     5461 gcggcgagcc tcaccggcgc ggccgccagc ccgtcgtctc gcgcaggcgc cggttcgaga
     5521 cgcggccgcc gtcgcgcacg gcgttgtacg tcccgctcgg cgcgtccagc gcggcgagaa
     5581 gcgcggcgcc cgcgtcgccc gggtcgagcg tgccgaaatg ggagtccgcc ccccgcccgc
     5641 cgaggagccc gaggcgcagg acgacgccgc gccggcccgc cgcggcgaac cgtgccgcct
     5701 cggcctcggc ggcgagggcc gaacggaggt gcggggccac gtcgcgcacg ggcgtctcct
     5761 cgtccacggc cccgtccttg ggatacacga acgtcacggc cggctgaacg tacactgccg
     5821 cgcggcccgc cagcgccgca tcgacgagga tccgcgaggc ctcggcgcgc agccggtcgt
     5881 tctcgcgcca ggcggacggg tcggccctgc gctcgggcgg cggaatgcgc gtggcgaggt
     5941 ggacgacggc gtcggcggga gcgacagcct cgcgcaccgc ttccgcgtcg aagaggtcga
     6001 gctcgtcgcg cgccggcgcg tcgacagggt ggctcgccgc cgcgagcagc ggcaggagcg
     6061 cgcgcccgag cacgccgctg cccccggtga cgaagacgcg cacgggggcg actgtacgct
     6121 ccgccgcatg ggcgcgatcc gcttcggccc gtcccgcctg ccgagccgcg agagcccgga
     6181 ggcggccgtc gagcttctcg tcgagcgcgg gttcaccgcg tgcgaggtcg acttcgaggg
     6241 cggcttctgg atgaagccgg aatgggagtg ggccgcgcgg ctcggcgagg cggcgcgcga
     6301 ggcggggatc gtcctctcgg ttcatgcgcc gatcgccgcg ttccccgggc acctgtcggg
     6361 cgacgagggg aagcgcaagc gcgccttcgg gatgctcgac cacacggccg gcgtcgcggt
     6421 cgcgctcggc gcggaggtgg tcgtcttcca ccccggcttc ctgctcgggc gctcgcgcga
     6481 ggaggcgctc gacgcggtcg tcagcgacct cgccgccctg cgcgagcggc tcgaggggaa
     6541 ggagcgcggg gtcccgttcg ggatcgagat catgggccgc gtccgggagc tcgggacggc
     6601 ggaggacgtc ttcacggtcg ccgcacgcct gccgtgggtg cggccggtgc tcgacttcgc
     6661 gcacctccac gcggtgacgg acggcggctt ccgcgccgcg gacgacttcg cggccatcct
     6721 cgggccggcc gacgagctgc tcgagcccgg cgcgccgttc cacatccact tctcggacat
     6781 cgcgttcgcg aaccgcaacg agacgaagca cctgccctac ggcgagggca ccctgcgggc
     6841 cgagccgctc gccgaggcgc tcgcccgctt cgaccgaccc gcgacggtga tctccgaatc
     6901 gccggatctc gcgagcgacc aggcgatcgg cgccgtcctc gcggcggcct agccgggcac
     6961 gacgccgcgc acggcccagg cgcgtgcctc gagctcgaac ggctcgtcgc cgactccgag
     7021 gcggcggcgg aacgcctcgc ggagggcggc ccggccgtcc tcgtcgagcg acgcggtgaa
     7081 cgcgccggag ggcgcgatgc ccgacgggaa cggctcccag aggtcgtcga agctctcgta
     7141 cgcggcccgc gcggtcagcg ccccggtccg gacctcgcgc aggccggcag actcccacaa
     7201 gcgaagcagc tcgccctcgg agcaccaccg catgacgacg ccctcgtccg cggccgcgcc
     7261 gcgctcgggc tcgacctcgc gggccgcgtc ccagaaggcg cgcaggagcg tcatctcgcc
     7321 cgcgtagtcc cagacgcacg acgcgacgac gccgtccggc cgggtgacgc gcgcgagctc
     7381 ccgaacgccc gcctccgcgt cgcgcatgaa gttcaggacg agctgcgaga gcgcggcgtc
     7441 gaacgcgtcg tcggggaacg ggagatcctc cgcggcggcg acggcgatct cggcgcccgg
     7501 cacgcgggag ctggccgcct cggcgaacgg ctcggacggc tccgcgccgg tgaccttctc
     7561 cgccccgagc cgctcggcga gcgcggcggt caggagcccc gtgccgcagc cgacgtcgag
     7621 cgcccgcatc ccgggctcga cgccggcgaa ccggatcagc ccggccgcga gcgacggccc
     7681 gtaccggccg accgcgcggt cgtatgcggc cgcggcgcgg aacgtgtcgg agccggccat
     7741 gggcggaggc tactccgacc tcgcggcggc gtacactggc accgatgacc gagcccgccg
     7801 agcatcccgc cgagcgcgac gagacgggca tgcgcgggcg ccgagcgagc ttccttcccc
     7861 cgcgcggcga gcgctggggg ctggagtcgc tcttcgtccg cgtcgtcgcg acgggcggga
     7921 tcatcgggat cggcgtcgcg ctcgccgcga tcctcgactg gcaagacgtc gcggcctgga
     7981 tcatcgggat cgtcgtctcg ggcgtgtccg tcctgctcgc cgcgctgctc tggtcgtcac
     8041 ggacgctctg accgtcttca cgatcggcca cggcgcccgc ccgcccgagg agctcgtcgc
     8101 ctgcctgatg gaggcaggcg tcgagacgct cgtcgacgtg cgccgctttc cctcctcgcg
     8161 gcggcacccg cacttcaacc gggacccgct cgcggcgacg ctcgaggagg cgggcatcgc
     8221 gtaccgccac gccgaggagc tcgggggccg gcgcggcgac cagccgggtg aggagcgctt
     8281 cgcgtgcatc caggtcgcgg ccttccggag ctacgtcgcg cggatgacga cgcccgagtg
     8341 gcaggaggcg ctcgaggcgg agctcgcgca gccggcgccg tgcttcatgt gcgcggagac
     8401 gccgtggtgg cggtgccatc gccggttcat cgccgagctc ctgaccgcgc gcggccacga
     8461 aattctccat ctc
//
LOCUS       DASTFC010000016         6049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1038546,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000016 DASTFC010000000
VERSION     DASTFC010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038546"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /gene="ruvB"
                     /locus_tag="VFO88_00480"
     CDS             complement(<1..689)
                     /gene="ruvB"
                     /locus_tag="VFO88_00480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET9544027.1"
                     /translation="MPERVTAAVLAPEDDDLERSLRPRRLEDFVGQERVKEQLSIALE
                     AAKGREEALDHVLLAGPPGLGKTTLAYVIREELGAGIRTVAGPALERKGDIAAILTSL
                     EPRDVLFVDEIHRLNRSVEEILYGALEDFRLDIVVGQGPAARTLTLDLPPFTLVGATT
                     RTGLLTTPLRDRFGITYRLDLYTGEELARIVRRSARILGVETDDEAAVEIARRARGTP
                     RVANRILRRVR"
     gene            complement(692..1273)
                     /gene="ruvA"
                     /locus_tag="VFO88_00485"
     CDS             complement(692..1273)
                     /gene="ruvA"
                     /locus_tag="VFO88_00485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET9544028.1"
                     /translation="MIARLRGAPAGAGADWLVLDVGGVGYRLSATPRAIQKADAASEV
                     ALETYLHVREDALQLYGFADAAERELFELLLAVNGVGPKVALAIVSGYSPSELRRAIV
                     REDAALFETIPGIGRKTAQRVVLELKEKIAPLVAVPGGAADAAASGDDAHLVAREALV
                     ELGYTVAEAEKRLAEIDPDLPAAERVRLALRAA"
     gene            complement(1270..1767)
                     /gene="ruvC"
                     /locus_tag="VFO88_00490"
     CDS             complement(1270..1767)
                     /gene="ruvC"
                     /locus_tag="VFO88_00490"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9544029.1"
                     /translation="MKVLGIDPGTAACGYGIVQTDGGRITAIAHGLWKTSAREVPALR
                     LKRIHDGVAELIAEHAPEAAALEESFVGADARTALFVGQARGAVLVACAAADVACAEY
                     PPATVKQSVCGYGRADKGQVGRMVAAILGLAEPPAPHHASDALAVAICHALASPLLAA
                     SGGAR"
     gene            1843..2763
                     /locus_tag="VFO88_00495"
     CDS             1843..2763
                     /locus_tag="VFO88_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544030.1"
                     /translation="MRPLPLALAVAACLAVPGTAAAQTLNGFVNADQTIGLTQGGTAV
                     TQLAPGTYSFEVNDTTAEHNFHLQGPGVNDATGIEATGIFGWPNRALTHGFYDYFCDV
                     HLAAMAGAFRVGNTLRVTKAGSGAGSSTVTSDPGGISCGATCTLAAPAGGMTTLTATP
                     GAQATFDGWSGGGCTGTGTCTVSLSGPVDVTATFTSTAPPPPPPPPPPPPGPSARVTG
                     VTVKKVRGVRVVAVKLSVTRRTAARAQVQRRGRTLASAQATFEPGARALKVRIAKRVK
                     AGAASVRTTLRDTAAGTSTVATRSIRLPRG"
     gene            2818..3714
                     /locus_tag="VFO88_00500"
     CDS             2818..3714
                     /locus_tag="VFO88_00500"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544031.1"
                     /translation="MRTRISMLALLALLLAAPPASAQTVLNAEVSGALTIWLRQGGVD
                     VTHLAPGSYTVNVNDISDQHNFRLRGPNGLEMRTDIWTVDPQVWTGLEFVDGLYHFNC
                     EHHAQMFGDFTVGNVIWLETTGLGTGKVTSAPGGLSWPQNRGAAFPAGSQVTLTATPD
                     AGSKFVGWSGGGCSGTGDCTVTVNGRVNVQAQFTPALTGGGTTAPATITRLAVVKTKA
                     GRVVRATLNVRRQTAVTAQLRKNARVLVSKSQQLSPGTRVVKLAVPRKAKAGAYTLRL
                     RLRDVAAGTVTTVNRSVRIPAP"
     gene            complement(3711..4466)
                     /locus_tag="VFO88_00505"
     CDS             complement(3711..4466)
                     /locus_tag="VFO88_00505"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9544032.1"
                     /translation="MSGHSKWSSIKHKKGAADAKRGKLFSKLSRAIIVAAREGGPDPD
                     GNISLANAIAKAREASMPKDTIERAIARGSGASADGETYESVVYEGYGPNGVAILVDA
                     LTDNRNRTAAEVRHIFAKHDGNLGASGAVAWLFERKALALVDGERYDEEEVMLAAAEG
                     GAEDVEPDGSSLRITAPPEALQTLREALDAAGIELESADLTMIPKTTVEVEEESTARK
                     VLRLMDALEENDDVQDVYANFDIPERVLEAVAP"
     gene            complement(4453..5046)
                     /gene="pdxT"
                     /locus_tag="VFO88_00510"
     CDS             complement(4453..5046)
                     /gene="pdxT"
                     /locus_tag="VFO88_00510"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET9544033.1"
                     /translation="MAEPLRIGVLAVQGDVREHAAMLRDAGAEVVEVRRPQELDGLDG
                     LVLPGGESTAIGRLVRLAGLEEPLRAFRGPVFGTCAGLILLAREASDGVPGQPLLGLL
                     DVAVRRNGYGRQVASFEADLGLAGEAAPLRGVFIRAPRITERGPGVEVLAELDGDPVL
                     VREGRILAASFHPELAGDPRVHERFLEIVREESCVGA"
     gene            complement(5074..5961)
                     /gene="pdxS"
                     /locus_tag="VFO88_00515"
     CDS             complement(5074..5961)
                     /gene="pdxS"
                     /locus_tag="VFO88_00515"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET9544034.1"
                     /translation="MQRETGTFTVKSGLAEMLKGGVIMDVTTAEQAKIAEDAGACAVM
                     ALERVPADIRRDGGVARMADATKVEEIQQVVSIPVMAKARIGHFVEAQILQSLGVDYI
                     DESEVLTPADEEYHIDKWAFNVPFVCGCRDIGEALRRISEGAAMIRTKGEAGTGDIVN
                     AVRHMRSVFGAIRRLQTLSGDELYAEAKELSAPLELVRWVAENGRLPVVTFTAGGIAT
                     PADAALCMQLGADGVFVGSGIFKSADPSTMAKAIVEATTNFRDAEVLARVSEGLGEPM
                     RGLSAAGLAPDELLEARGW"
BASE COUNT          789 a         2303 c         2151 g          806 t
ORIGIN      
        1 tcgcgcacgc ggcggaggat ccggttcgcg acccgcggcg tgccccgcgc gcgccgggcg
       61 atctcgaccg ccgcctcgtc gtccgtctcc acgccgagga tccgcgccga gcggcgcacg
      121 atccgggcca gctcctcgcc cgtgtagagg tcgagccggt acgtgatccc gaagcggtcg
      181 cggagcggcg tcgtcaggag cccggtccgc gtcgtcgcgc cgacgagcgt gaacggcggc
      241 aggtcgagcg tgagcgtccg cgccgccggg ccctgcccga cgacgatgtc gagccggaag
      301 tcctcgagcg cgccgtagag gatctcctcg accgagcggt tcagccggtg gatctcgtcc
      361 acgaagagca cgtcgcgcgg ctcgagcgag gtgaggatgg cggcgatgtc gcccttgcgc
      421 tcgagcgcgg ggccggccac cgtgcggatc ccggcgccga gctcctcccg gatcacgtag
      481 gcgagcgtcg tcttcccgag ccccggcggg ccggccagga ggacgtggtc gagcgcctcc
      541 tcccgcccct tcgccgcctc gagcgcgatc gagagctgct ccttgacgcg ctcctggccg
      601 acgaagtcct cgagccggcg cgggcggagc gagcgctcga ggtcgtcgtc ctccggcgcg
      661 aggacggccg ccgtgacccg ctccggcacg ctcatgccgc tcgcagggcc agccgcacgc
      721 gctccgcagc gggcaggtcg gggtcgatct ccgcgaggcg cttctccgcc tcggcgacgg
      781 tgtagccgag ctcgacgagc gcctcgcgcg cgacgaggtg cgcgtcgtcg cccgaggccg
      841 cggcgtccgc ggcgccgccc ggcacggcga cgagcggcgc gatcttctcc ttcagctcga
      901 ggacgacgcg ctgcgcggtc ttgcgcccga tccccgggat cgtctcgaag agcgccgcgt
      961 cctcacgcac gatcgcccgg cgaagctcgc tcggcgagta gcccgacacg atcgcgagcg
     1021 cgaccttcgg gcccacgccg ttcacggcga ggaggagctc gaagagctcg cgctcggccg
     1081 cgtcggcgaa gccgtacagc tggagcgcgt cctcgcgcac gtggaggtac gtctccaggg
     1141 cgacctcgct cgccgcgtcc gccttctgga tcgcgcgcgg cgtggcgctc aggcgatagc
     1201 cgaccccgcc gacgtcgagg acgagccagt cggcgcccgc cccggccggc gcgccccgga
     1261 gccgcgcgat caccgagcgc ctcccgacgc ggccaagagg ggcgaggcga gcgcgtggca
     1321 gatcgcgacg gcgagcgcgt cggaggcatg gtgcggcgcg ggcggctcgg cgagcccgag
     1381 gatcgcggcg accatccggc cgacctggcc cttgtcggcc cgcccgtagc cgcacacgga
     1441 ctgcttcacg gtcgccggcg ggtactcggc gcacgccacg tcggccgccg cgcacgcgac
     1501 gagcacggcg ccgcgcgcct gcccgacgaa gagcgcggtg cgggcgtcgg cgccgacgaa
     1561 ggactcctcg agcgcggccg cctccggcgc gtgctcggcg atgagctccg cgaccccgtc
     1621 gtggatccgc ttcaagcgga gcgcgggcac ctcgcgcgcc gacgtcttcc acaggccgtg
     1681 cgcgattgcc gtgatgcgcc ctccgtctgt ctggacgatg ccgtatccgc atgccgccgt
     1741 gcccgggtcg atgccgagaa ccttcacgaa gcgaggttag ggacgagccc ggacgttcct
     1801 gcccgggcaa accgcctgcc tcgcgcgcgc gtagaggacg gcgtgaggcc ccttcctctc
     1861 gcgctcgccg tcgccgcgtg cctggcggtt ccgggcacgg ccgccgcgca gacgctgaac
     1921 gggttcgtga acgccgacca gacgatcggg ctgacgcagg gcggcacggc ggtcacgcag
     1981 ctcgccccgg gcacgtactc gttcgaggtg aacgacacga cggcggagca caacttccac
     2041 ctgcagggcc cgggcgtgaa cgacgcgacg gggatcgagg cgaccggcat cttcggctgg
     2101 ccgaaccggg cgctcacgca cgggttctac gactacttct gcgacgtcca tctcgccgcc
     2161 atggcgggcg cgtttcgcgt ggggaacacg cttcgggtga cgaaggccgg aagcggcgcc
     2221 ggctcgagca cggtcacgag cgaccccggc gggatcagct gcggcgcgac gtgcacgctc
     2281 gcggcgccgg ccggcgggat gacgacgctg accgcgacgc ccggcgcgca ggcgacgttc
     2341 gacgggtggt ccggcggcgg ctgcaccggc accggcacgt gcacggtgag cctgagcggc
     2401 cccgtcgacg tgaccgcgac cttcacctcg acggcgccgc ctccgcctcc tcctccgccg
     2461 ccgcctccgc cggggccgtc cgcccgggtg accggcgtga cggtgaagaa ggtgcgcggc
     2521 gtccgcgtcg tcgccgtgaa gctttcggtc acacgccgca cggccgcacg cgcgcaggtc
     2581 cagcgccgcg ggcggacgct cgcctccgcg caggcgacgt tcgagcccgg cgcccgggcg
     2641 ctcaaggtgc gcatcgcgaa gcgggtcaag gccggcgccg cgagcgtccg gacgaccctc
     2701 cgcgacaccg ccgccggcac ctcgacggtc gcgacgcggt cgatccggct cccgcgcgga
     2761 taaaccgcac cattgcactt gtttcggacc ggcgggcctg gtatcgtcgg cgcgcccatg
     2821 aggaccagga tctcgatgct ggccctgctc gccctgctgc tcgccgcccc gcccgcgtca
     2881 gcgcagaccg tcctgaacgc cgaggtgtcg ggcgcgctca ccatctggtt gcgccagggc
     2941 ggcgtcgacg tcacgcacct ggcccccggc tcgtacacgg tgaacgtcaa cgacatctcg
     3001 gaccagcaca acttccgcct cagaggcccg aacggcctcg aaatgcgcac cgacatctgg
     3061 acggtcgacc cgcaggtctg gaccggcctc gagttcgtcg acggcctcta ccacttcaac
     3121 tgcgagcacc acgcgcagat gttcggtgac ttcacggtcg ggaacgtcat ctggctcgag
     3181 accacggggc tcgggaccgg gaaggtcacg agcgcgcccg gcggcctgag ctggccgcag
     3241 aaccgcggcg cggccttccc ggccgggagc caggtgacgc tgaccgcgac gccggacgcc
     3301 ggctcgaagt tcgtcggctg gtcgggcggc ggctgcagcg ggaccggcga ctgcacggtg
     3361 accgtgaacg gccgcgtcaa cgtccaggcg cagttcacgc cggcgctcac cggcggcggc
     3421 acgaccgctc ccgcgacgat cacgcgcctc gcggtcgtga agacgaaggc cgggcgcgtg
     3481 gttcgtgcga ccctgaacgt gcggcgccag accgcggtga ccgcgcagct gcggaagaac
     3541 gcccgcgtgc tcgtctcgaa gtcgcagcag ctctcgcccg gcacgcgggt cgtcaagctc
     3601 gccgtcccgc ggaaggcgaa ggcgggcgcc tacacgctcc gcttgcgtct gcgcgacgtc
     3661 gccgcgggca cggtcacgac cgtcaaccgg tcggtccgga tcccggcgcc ctagggcgcg
     3721 accgcctcga gcacgcgctc ggggatgtcg aagttcgcgt agacgtcctg gacgtcgtcg
     3781 ttctcctcga gcgcgtccat caggcggagc accttccgcg cggtcgactc ctcctccacc
     3841 tccaccgtcg tcttcgggat catcgtcagg tcggcgctct cgagctcgat cccggccgcg
     3901 tcgagcgcct cgcgaagcgt ctggagcgcc tccggcggag ccgtgatccg caggctcgac
     3961 ccgtcgggct cgacgtcctc cgccccgccc tcggcggcgg cgagcatgac ctcctcctcg
     4021 tcgtagcgct ctccgtccac gagcgcgagc gccttgcgct cgaagagcca ggcgacggcg
     4081 ccgctcgcgc cgaggttccc gtcgtgcttc gcgaagatgt ggcgcacctc ggccgcggtg
     4141 cggttccggt tgtcggtgag cgcgtcgacg aggatcgcga cgccgttcgg gccgtagccc
     4201 tcgtacacga cgctctcgta ggtctcgccg tccgcgctcg cgcccgagcc gcgggcgatc
     4261 gcgcgctcga tggtgtcctt cggcatcgag gcctcgcgcg ccttcgcgat cgcgttcgcg
     4321 agcgagatgt tgccgtccgg gtcggggccg ccctcgcggg cggcgacgat gatcgcgcgg
     4381 gagagcttcg agaagagctt gccgcgcttc gcgtccgccg cgcccttctt gtgcttgatg
     4441 gacgaccact tgctatgccc cgacacagct ctcctccctc acgatctcca ggaagcgctc
     4501 gtggacgcgc gggtcgccgg cgagctccgg gtggaacgac gcggcgagga tccgcccctc
     4561 gcggacgagc accgggtcgc cgtcgagctc ggcgagcacc tcgacgcccg gcccgcgctc
     4621 ggtgatccga ggcgcgcgga tgaagacgcc gcgcagcggc gccgcctcgc cggcgagccc
     4681 gaggtcggcc tcgaagctcg ccacctggcg cccgtacccg ttccgccgca cggcgacgtc
     4741 gagcagcccg aggagcggct ggcccggcac gccgtccgac gcctcgcgcg cgagcaggat
     4801 gaggcccgcg cacgtcccga agaccggccc gcggaaggcg cgcagcggct cctcgagccc
     4861 ggcgaggcgg acgagccgcc cgatcgcggt cgactcgccg cccgggagga cgagcccgtc
     4921 gagcccgtcg agctcctgcg gccggcgcac ctcgacgacc tccgcgcccg cgtcgcggag
     4981 catcgccgca tgctcccgga cgtcgccctg gactgcgagc acgccgatgc gaagcggttc
     5041 ggccacgtcc cgactatagc cccggaggcg ctgctaccag ccgcgcgctt cgagcagctc
     5101 gtcgggcgcg aggccggcgg cggagaggcc ccgcatgggc tcgcccaggc cctcggaaac
     5161 gcgcgccagc acctcggcgt cgcggaagtt cgtcgtcgcc tcgacgatcg ccttcgccat
     5221 ggtcgacggg tcggcgctct tgaagatccc ggagccgacg aagacgccgt ccgcgccgag
     5281 ctgcatgcag agcgccgcgt cggccggcgt cgcgatcccg cccgcggtga acgtgacgac
     5341 cgggaggcgt ccgttctcgg ccacccaccg gacgagctcg agcggcgcgc tgagctcctt
     5401 cgcctcggcg tagagctcgt cgccggagag cgtctgcagc cggcggatcg cgccgaacac
     5461 ggagcgcatg tgccggacgg cgttcacgat gtcgcccgtc ccggcctcgc ccttcgtccg
     5521 gatcatcgcc gcgccctcgg agatgcggcg gagcgcctcg ccgatgtcgc ggcacccgca
     5581 cacgaacggg acgttgaacg cccacttgtc gatgtggtac tcctcgtccg ccggcgtgag
     5641 gacctcggac tcgtcgatgt agtccactcc gagggactgc aggatctgcg cctcgacgaa
     5701 gtgcccgatc cgcgccttcg ccatcaccgg gatcgagacc acctgctgga tctcctcgac
     5761 cttcgtcgcg tcggccatcc gggcgacgcc gccgtcccgc cggatgtccg cggggacgcg
     5821 ctccagggcc atcaccgcac aggctccggc gtcctcggcg atcttcgcct gctcggccgt
     5881 cgtgacgtcc atgatcacgc cgcccttgag catctcggcc aggccggact tcacggtgaa
     5941 ggtgccggtc tcgcgctgca cgggatatcc ctcctcgtcc gagctgtgcg gagtctaccg
     6001 ccgcccccgc accgggcagc gggctcggct cagcgctcga acgagacct
//
LOCUS       DASTFC010000017         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_214519,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000017 DASTFC010000000
VERSION     DASTFC010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214519"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1559
                     /locus_tag="VFO88_00520"
     CDS             144..1559
                     /locus_tag="VFO88_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET9544035.1"
                     /translation="MTAPLAVSLETVNLLYAVSAVLLVFGIKQLSSPRTARQGNFVAA
                     AGMAIAVGITLFHPDVEKYGLMAVGIAVGTVIGVVSARAVKMTAIPQMVALFNGVGGG
                     AVALIAAAEYHRLAPDLGALPVDVIAGILFGALVGSISFAGSLVAFGKLQELLPGRPL
                     TFPGQIVVNGLLLVLAIACGVGAGVTEDGIWIVLLIVLALVLGAGAVLPIGGADMPVV
                     ISLLNAFTGLAAASSGFVLGNNALIIGGTLVGASGTLLTILMGRAMNRSIANVLFGAF
                     GAAPAAGPAAAPGASGDGAMVRQTTAEDTAVMLAYASQVMVVPGYGLAVAQAQHVTRE
                     LADLLEARGVRVRYAIHPVAGRMPGHMNVLLAEANVPYPALLDMDDANPELNRTDVAL
                     VVGANDVTNPAARSNPESPLYGMPILDVDHAKNVVVLKRSMNPGFAGIDNELYTNPKT
                     AMLFGDAKDSLERLVAAVKAV"
     gene            complement(1600..1875)
                     /locus_tag="VFO88_00525"
     CDS             complement(1600..1875)
                     /locus_tag="VFO88_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9544036.1"
                     /translation="MGIIGWILLGLLAGVIAKAILPGDDPGGIIVTTIIGIVGALLGG
                     LIAGVLGLGDPLDEFFDISTWIAAIIGAIVLLLIYRLVVGRGRTRTV"
     gene            complement(1957..>2891)
                     /locus_tag="VFO88_00530"
     CDS             complement(1957..>2891)
                     /locus_tag="VFO88_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9544037.1"
                     /translation="DGRVSEEAARDAADESIVVGKGGWLRLSEELLRRAGIGSHAAAR
                     LEAGSIMVEAVGDVLPLEAPERPAELAPAPARSGAPVAELRGVAKRYGEGHIARPVFA
                     RVDATFAAGRLYALTGPSGTGKTTLLHLLAGLELPTEGEVVVAGTSLAGLDRAGRAAF
                     RRERVALVGQEPGLVPTLTARENVELGLALRGVPAADAAARAAEALAAVGVAGRAEGR
                     VAVLSAGERERVAVARAVAARPALLLADEPTARLDEANGLAVAALLARLARESGAAVV
                     CATHDPIVVEQADEVVPLARLDAPDHTVEAVTKV"
BASE COUNT          382 a         1079 c         1046 g          384 t
ORIGIN      
        1 gactcgacgc tcgagcaggt gctcgcgttc gtcgccgtcg tcttcgccac ggtgaacgtc
       61 gtcggcggct ttctcgtcac cgaccggatg ctccacatgt tcaagggcaa gcggccggag
      121 gggcccccgg aggacgacgt cccgtgaccg cgccgctcgc cgtctcgctc gagacggtca
      181 acctcctgta cgcggtctcg gcggtgctgc tcgtcttcgg gatcaagcag ctctcgtcgc
      241 cgcgcacggc ccgccagggg aacttcgtcg ccgccgccgg gatggcgatc gcggtcggga
      301 tcacgctctt tcaccccgac gtcgagaagt acggcctcat ggcggtcggg atcgccgtgg
      361 gaacggtgat cggggtcgtc tcggcgcgcg cggtcaagat gaccgcgatc ccgcagatgg
      421 tggcgctctt caacggcgtc ggcggcgggg cggtcgccct catcgcggcc gcggagtacc
      481 accggctcgc gccggacctc ggcgcgctgc cggtggacgt gatcgccggg atcctcttcg
      541 gcgcgctcgt cggctcgatc tcgttcgccg ggagcctcgt cgccttcggg aagctccagg
      601 agctgctccc cggccggccg ctcacgttcc ccgggcagat cgtcgtgaac gggctgctgc
      661 tcgtcctcgc gatcgcgtgc ggcgtcggcg ccggggtcac ggaggacggg atctggatcg
      721 tcctcctcat cgtgctcgcg ctcgtgctcg gcgcgggagc cgtgctcccg atcggcggcg
      781 cggacatgcc ggtcgtgatc tcgctcctga acgccttcac cgggctcgcg gccgcctcga
      841 gcggcttcgt gctcgggaac aacgcgctca tcatcggcgg gacgctcgtg ggcgcctcgg
      901 ggacgctcct cacgatcctc atgggccggg cgatgaaccg ctcgatcgcg aacgtcctct
      961 tcggggcgtt cggcgcggcg cccgcggccg ggccggcggc ggcgcccggc gcgagcggcg
     1021 acggcgcgat ggtgcgccag acgaccgcgg aggacacggc cgtcatgctc gcgtacgcga
     1081 gccaggtgat ggtcgtccct ggctacgggc tcgcagtcgc ccaggcgcag cacgtgacgc
     1141 gcgagctcgc cgacctgctc gaggcgcgcg gcgtccgcgt gcgctacgcg atccacccgg
     1201 tcgcgggccg gatgcccggg cacatgaacg tcctgctcgc cgaggcgaac gtgccgtacc
     1261 cggcgctcct cgacatggac gacgcgaacc cggagctcaa ccgcaccgac gtcgcgctgg
     1321 tcgtcggcgc gaacgacgtg acgaacccgg ccgcgcgctc gaacccggag agcccgctct
     1381 acgggatgcc gatcctcgac gtcgaccacg cgaagaacgt cgtcgtgctc aagcgctcga
     1441 tgaacccggg cttcgcggga atcgacaacg agctctacac gaacccgaag accgcgatgc
     1501 tcttcggcga cgcgaaggac tcgctcgagc gcctcgtcgc cgccgtcaag gcggtgtgac
     1561 gggccgggac ggcaggacgc cgccccggcc cgacgcgcgt cagaccgttc gggtgcgccc
     1621 gcgccccacg acgagccggt agatcagcag caagacgatc gcgccgatga tggcggcgat
     1681 ccaggtgctg atgtcgaaga actcgtcgag cgggtcaccg agaccgagca ccccggcgat
     1741 cagtccgccg agcagcgcgc cgacgatccc gatgatcgtg gtcacgatga tcccgccggg
     1801 gtcgtccccg ggaaggatcg ccttcgcgat gacgccggcg agcaagccga gcaggatcca
     1861 gccaatgatt cccatgtgtt tctctccttt ttccgtcgcg tgccgccacg tcgtatgccc
     1921 agtcggcaac caacgtaacg tcgcccggga cgatgctcac accttcgtga ccgcctcgac
     1981 cgtgtgatcc ggagcatcca gccgcgcgag cggcacgacc tcgtcggcct gctcgacgac
     2041 gatcggatcg tgcgtcgcgc agacgacggc ggcgcccgac tcgcgcgcga ggcgggcgag
     2101 aagggcggcg acggcgagcc cgttcgcctc gtcgaggcgc gcggtcggct cgtccgcgag
     2161 gaggagcgcg ggccgggcgg ccacggcacg ggcgacggcg acccgctccc gctcgcccgc
     2221 ggagaggacc gcgacccggc cctcggcccg tccggccacg ccgacggcgg cgagcgcctc
     2281 cgcggcgcgc gcggcggcgt cggcggccgg gacgccgcgc agcgcaaggc cgagctcgac
     2341 gttctcccgg gccgtgagcg tcgggacgag gcccggctcc tggccgacga gcgcgacccg
     2401 ctcgcgccgg aacgccgccc ggccggcgcg gtcgagcccg gcgaggctcg tgccggcgac
     2461 gacgacctcg ccctcggtcg ggagctccag cccggcgagc aggtgcagga gcgtcgtctt
     2521 cccggttccg gacgggccgg tgagcgcgta gagccgcccg gcggcgaacg tcgcgtctac
     2581 ccgcgcgaac accggccggg cgatgtggcc ctcgccgtag cgcttcgcca cgccgcgcag
     2641 ctcggcgacc ggagcgcccg aacgggcggg cgccggggcg agctcggcgg gacgctcggg
     2701 cgcctcgagc ggaaggacgt ccccgaccgc ctcgaccatg atcgagcccg cctcgaggcg
     2761 cgcggccgcg tgcgagccga tcccggcgcg ccggagaagc tcctccgaga gccgcagcca
     2821 gccgcccttg ccgacgacga tcgactcgtc cgccgcgtcg cgggcggcct cctcgctcac
     2881 ccggccgtcg c
//
LOCUS       DASTFC010000018         5955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1137397,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000018 DASTFC010000000
VERSION     DASTFC010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137397"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3542)
                     /locus_tag="VFO88_00535"
     CDS             complement(<1..3542)
                     /locus_tag="VFO88_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9544038.1"
                     /translation="MQTPERTPLAGALSWAAGTVALAVVAPWQTVLGRRKRRGGVVEE
                     APPEVSAVPLGGRLARAETAQEVVDALFDAVEGLPGSEAVALALVDEARTRAAGFAAR
                     GTDEERWRGISVNLERESGAIATAARERAAFAVYDVASSPTVSPSVAEAIGARSAAFV
                     PLVAEDAAVAVLVLATTSEPRFFSSAELEAVRRLADEAAPVLARLSSASALRRALERE
                     KLVAEIARKVRSELDLDDVLQVAVTETGRGLDVARCFIRFGDEEGQLPLRAEWRRAGL
                     EPITRAAQLPVANLALREGRTMAIGDIDAARELEDPSLGSVELLRQLGTRAVLATPIV
                     VFDRTIGVFGLHRTEAGAWSASEVGVAEAIAGEIGIAVHAARLLEEDERRLGLQEALL
                     KAAQVVTSDLRFESVLRRLVDEVAILFGADAADCWMFEPGTGLLRSRAVYGLPEDELD
                     RTIPVLGWQARAIESAAPVLARDFARTENPPPSENYADFEEIMIAPMIWLGEVRGVLG
                     VCSREAGRFDSSELEILEAFARFASLASHNAESYEERARQAQVQQGFYRIAEALGSPL
                     SLAQTLDALGRAAAEALGGETAAVLSGRGGRLRLDGSYRLPPRLAERLRDGLPGSSTP
                     LAAAAGDGKIVTSGVLRDDDRFDTEARRLLAEHGYGALLAAPVQRASGENVVVVVLFG
                     AERTFTDDDLVLARQLGAAARGALERSELFEAERRARGLSERLASVGARLVTSLAPER
                     VLEEVAAEAVELLDADAAVLRALDGEELVVLSAAGAAPEGLAGTRSSSGQGVLGDVIQ
                     SRRAVAVEDVRETPRRGRGDPVLEEAPACIAVPMAAPGGGLHGVLSVYGAEPRAWRGD
                     EVQALVALAAMASSSLANAELYRSVAEEKERSEAILGNIADGIVAVDREDRIVLWNSM
                     AERITGVPASEALGRRVAETLQRELASGEEEPKGERAVAIVRGGKEVWLSLTEAVMRD
                     GGGAVGGRIFAFRDVSGERAVEQMKSDFVATVSHELRTPLTSIYGFAETLMRGDVAFD
                     ENERATFLGYIASESERLIRIVDDLLNVARLEAGMLGLTLSTTDVSAVVRSAVESVRE
                     QADGRVALQADLPDGPLHAEADPARLAEVVRHLLENALRASPEGGTVRVGARRRSDAV
                     ELRVTDEGEGFAPVDRQRIFTKFFRAHGAEPGDE"
     gene            3803..4756
                     /locus_tag="VFO88_00540"
     CDS             3803..4756
                     /locus_tag="VFO88_00540"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET9544039.1"
                     /translation="MVKRILLASPRGYCAGVERAVDTVEQALDLWGPPVYVRKQIVHN
                     IHVVRDLEERGAIFVETETEVPPGATVVFSAHGVAPSVHENARRLELKTIDATCPLVT
                     KVHSEARHYAKAGYTIVLVGHAGHEEVEGTMGEAPDSIVLVESQEEAETVELPDGPLA
                     YITQTTLSVDETNGVIDVLRRRFPRIEAPKKEDICYATTNRQRAVKAMLGSIDLLLVI
                     GSRNSSNSNRLVEVARAAGVMSYLVDDETEIEEAWLDGVETVGVTSGASAPERLVERV
                     LAWFAARGVTDVSPFAEEREDIFFKLPAQVRPEAAAGTPSG"
     gene            complement(4701..5327)
                     /locus_tag="VFO88_00545"
     CDS             complement(4701..5327)
                     /locus_tag="VFO88_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9544040.1"
                     /translation="MARPLRPQPPGGIHHVMARGVAGLPLFRTDGDRDLFLATVEDTV
                     LRHEWICLAYVVLGTHYHLLVRTPEPDDLAPGMKRLNGNYAQSFNRRHRGRGHVFESR
                     YASVLVESDGHLRWLFRYLAWNPVNAGLCDDPKDWPWSSYPRLLGLRHARPPRFLAVD
                     AALGLFGRTRTHARARLRDFVEGAAAGPAALSRTASRPPLPGEPARAA"
     gene            complement(5676..>5955)
                     /locus_tag="VFO88_00550"
     CDS             complement(5676..>5955)
                     /locus_tag="VFO88_00550"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HET9544041.1"
                     /translation="GREGFAVAADDGITVSLDTALDDELRLEGRVHDLIHRVNGMRKE
                     AGLELTDRITLTLPADDHDALADHTDWIARETLAVRVEPGDDLAVARA"
BASE COUNT          804 a         2158 c         2245 g          748 t
ORIGIN      
        1 tgctcgtcgc ccggctcggc gccgtgcgcg cggaagaact tcgtgaagat ccgctgacgg
       61 tcgaccggtg cgaacccctc gccctcgtcc gtcacgcgga gctcgaccgc gtcggaccgg
      121 cggcgcgcgc cgacacgcac ggtgccgccc tcgggcgagg cgcggagcgc gttttcgagc
      181 aggtggcgca cgacctccgc gagccgggcc ggatcggcct ccgcgtggag cggcccatcc
      241 ggcaggtcgg cctggagcgc gacgcgcccg tccgcctgct cccgcacgct ctcgaccgcg
      301 gagcgcacga ccgcggagac gtccgtcgtc gacagggtga ggccgagcat cccggcctcg
      361 agacgggcga cgttcaggag gtcgtcgacg atccggatga gccgctcgga ctcggaggcg
      421 atgtagccga ggaacgtcgc gcgctcgttc tcgtcgaacg ccacgtcgcc gcgcatgagc
      481 gtctccgcga agccgtagat cgaggtgagc ggcgtgcgga gctcgtgcga gaccgtggcg
      541 acgaagtcgg acttcatctg ctcgaccgcc cgctcgccgg agacgtcgcg aaacgcgaag
      601 atccgcccgc cgaccgcgcc cccgccgtcg cgcatgacgg cctccgtgag cgagagccag
      661 acctccttcc cgccgcggac gatcgcgacg gcgcgctcgc ccttcggctc ctcctccccg
      721 ctcgcgagct cgcgctggag cgtctcggcg acgcgccggc cgagcgcctc ggacgcgggg
      781 acgccggtga tccgctcggc catcgagttc cagaggacga tccggtcctc gcggtcgacg
      841 gccacgatcc cgtcggcgat gttgccgagg atcgcctcgc tccgctcctt ctcctcggcg
      901 accgaccggt agagctccgc gttcgcgagg ctcgaggagg ccatcgcggc gagggcgacg
      961 agcgcctgca cctcgtcgcc gcgccaggcg cgcggctcgg cgccgtagac gctgagcacg
     1021 ccgtggaggc cgccgcccgg cgccgccatc gggacggcga tgcacgcggg cgcctcctcg
     1081 agcaccgggt cgccgcggcc gcggcgcggg gtctcgcgca cgtcctcgac ggcgaccgcg
     1141 cggcgcgact ggatcacgtc gccgaggacg ccctggcccg agctcgaccg cgtgccggcg
     1201 agcccttccg gcgcggcgcc cgcggccgag aggacgacga gttcctcgcc gtcgagcgcc
     1261 cggaggacgg ccgcgtcggc gtcgagcagc tcgaccgcct cggcggccac ctcctcgagc
     1321 acgcgctcgg gcgcgaggct cgtgacgagc cgcgcgccga cgctcgcgag gcgctcggag
     1381 agcccgcgcg cacgccgctc ggcctcgaag agctcgctcc gctcgagcgc gccgcgggcg
     1441 gccgcgccga gctggcgcgc gagcacgagg tcgtcgtccg tgaacgtccg ctcggcgccg
     1501 aagagcacga cgacgacgac gttctcgccg ctggcccgct gcacgggcgc cgcgaggagg
     1561 gctccgtagc cgtgctcggc gagcaggcgg cgggcctccg tgtcgaagcg gtcgtcgtcg
     1621 cggagcacgc cggaggtgac gatcttcccg tcgccggccg ccgcggcgag cggcgtcgag
     1681 gagccgggca ggccgtcgcg gagccgctcc gcaaggcgcg gcggaagccg gtacgagccg
     1741 tcgagccgca gccgcccgcc gcgtcccgag agcaccgcgg ccgtctcgcc gccgagcgcc
     1801 tccgccgcgg cgcgcccgag cgcgtcgagc gtctgcgcga gggagagcgg cgagccgagc
     1861 gcctcggcga tccggtagaa gccctgctgc acctgggcct ggcgcgcgcg ctcctcgtag
     1921 ctctccgcgt tgtgcgaggc gagcgacgcg aagcgggcga aggcctcgag gatctcgagc
     1981 tcggaggagt cgaaccgccc ggcctcgcgg gagcagacgc cgaggacgcc gcgcacctcg
     2041 ccgagccaga tcatcggcgc gatcatgatc tcctcgaagt ccgcgtagtt ctcgctcgga
     2101 ggcgggttct cggtgcgtgc gaagtcacgc gcgaggacgg gagcggcgct ctcgatcgcg
     2161 cgcgcctgcc agccgaggac ggggatcgtc cggtcgagct catcctcggg gagcccgtag
     2221 accgcacgcg agcggagcag gcccgtcccg ggctcgaaca tccagcagtc ggcggcgtcg
     2281 gcgccgaaga ggatcgccac ctcgtccacg aggcggcgga gcaccgactc gaaccggagg
     2341 tcgctcgtga cgacctgggc cgccttgagc agcgcttcct ggaggccgag gcgccgctcg
     2401 tcctcctcga gcaggcgcgc ggcgtggacg gcgataccga tctcccccgc gatcgcctcg
     2461 gcgaccccca cctcgctcgc cgaccaggcg ccggcctccg tgcggtggag cccgaagacg
     2521 ccgatcgtgc ggtcgaagac gacgatcggc gtggcgagga cggcccgggt tccgagctga
     2581 cggaggagct cgacgctccc gagcgaggga tcctcgagct cgcgcgccgc gtcgatgtcg
     2641 ccgatcgcca tcgtgcgccc ctcccggagc gcgaggttcg cgaccgggag ctgcgcggcc
     2701 cgggtgatcg gctcgaggcc ggcgcgccgc cactcggcgc ggagcggcag ctggccctcc
     2761 tcgtcgccga agcggatgaa gcagcgcgcg acgtcgagcc cgcggcccgt ctccgtgacc
     2821 gcgacctgga ggacgtcgtc gaggtcgagc tcggagcgga ccttgcgggc gatctcggcg
     2881 acgagcttct cgcgctcgag ggcccggcgg agtgcgctcg cgctgctcag gcgcgcgagg
     2941 accggcgccg cctcgtcggc caggcgccga acggcctcga gctcggcgga ggagaagaag
     3001 cgcggctcgg aggtcgtggc gaggacgagc acggcgaccg ccgcgtcctc ggcgacgagc
     3061 gggacgaacg cggcgctccg tgcgccgatc gcctccgcga cggacgggct caccgtcggc
     3121 gaggacgcga cgtcgtacac ggcgaacgcg gcgcgctccc gcgccgcggt ggcgatcgcg
     3181 ccggactcgc gctcgaggtt caccgagatt ccccgccagc gctcctcgtc ggttccccgc
     3241 gcggcgaacc cggcggcccg ggtgcgcgcc tcgtccacga gcgcgagcgc gaccgcctcg
     3301 ctcccgggga ggccctcgac cgcgtcgaag agcgcgtcca cgacctcctg ggcggtctcc
     3361 gcccgggcga gccgcccgcc gagcggcacg gccgagacct cgggaggcgc ctcctcgacg
     3421 acgcctccgc gccgcttccg gcggccgagc acggtctgcc acggcgcgac gacggcgagc
     3481 gcaacggtgc cggccgccca gctcagggca ccggcaagcg gagtgcgttc gggcgtctgc
     3541 atggcggtcg ggcgtctccc ccgtccggat gataggagcg gggaaacatc gccgccggat
     3601 cgcggaaaga aatccgagag aagactggca gaacttggct gaaagcgata cggtctcccg
     3661 cactgggcga tgcgagccgc ggcaggtgtc ctggccgagt acgccgcggt gagcgatgcg
     3721 gagggcccgc cgctataccc cccagcggtg ggccttccgc ttctttcaag gggaaggcgc
     3781 cgccggggat accatgggag ccatggtgaa gcgcatcctc ctcgcctcgc ccaggggcta
     3841 ctgcgcaggc gtcgagcggg ccgtcgacac ggtcgagcag gcgctcgacc tctggggccc
     3901 gcccgtctac gtgcggaagc agatcgtcca caacatccac gtcgtccgcg atctcgagga
     3961 gcggggcgcg atcttcgtcg agacggagac ggaggtcccg cccggcgcga cggtggtctt
     4021 ctccgcccac ggcgtcgcgc cctcggtgca cgagaacgcg cgccggctcg agctgaagac
     4081 gatcgacgcg acgtgcccgc tcgtgacgaa ggtgcactcc gaggcgcgcc actacgcgaa
     4141 ggcgggctac acgatcgtgc tcgtcggcca cgccggccac gaggaggtcg aggggacgat
     4201 gggcgaggcg ccggactcga tcgtgctcgt cgagtcgcag gaagaggccg agacggtcga
     4261 gctcccggac gggccgctcg cgtacatcac gcagacgacg ctctcggtcg acgagaccaa
     4321 cggggtgatc gacgtgctgc ggcgccgctt cccgcgaatc gaggcgccga agaaggagga
     4381 catctgctac gcgaccacga accggcagcg cgccgtcaag gcgatgctcg gctcgatcga
     4441 cctcctcctc gtgatcggct cgcgcaacag ctcgaactcg aaccggctcg tcgaggtggc
     4501 gcgcgcggcg ggggtcatgt cgtacctcgt cgacgacgag acggagatcg aggaggcgtg
     4561 gctcgacggg gtcgagacgg tcggcgtcac gtcgggggcc tcggcgcccg agcggctcgt
     4621 cgagcgggtg ctcgcctggt tcgcggcgcg cggcgtcacg gacgtctcgc ccttcgcgga
     4681 ggagcgcgag gacatcttct tcaagctgcc cgcgcaggtt cgcccggaag cggcggccgg
     4741 gacgccgtcc ggctgagcgc ggccgggccg gctgcggctc cctcgacgaa gtctctgagc
     4801 ctggcgcggg cgtgcgtacg ggtgcgcccg aagagaccga gtgcggcgtc tacggcgagg
     4861 aagcgcggcg ggcgggcgtg ccggaggccg agcaggcgcg ggtagctgct ccagggccag
     4921 tccttcgggt cgtcgcacag gccggcgttc accgggttcc acgcgaggta gcggaagagc
     4981 cagcgcaggt ggccgtcgct ctcgacgagg acggacgcgt accggctctc gaagacatgg
     5041 ccccggccgc ggtggcggcg gttgaagctc tgggcgtagt tgccgttcag gcgcttcatc
     5101 ccgggcgcga ggtcgtccgg ctccggcgtc ctgacgagga ggtggtagtg cgtgccgagg
     5161 acgacgtacg cgaggcagat ccactcgtgg cgcaggacgg tgtcctcgac cgtggcgagg
     5221 aagaggtcgc ggtccccgtc cgtgcggaag agcgggaggc cggcgacgcc gcgcgccatc
     5281 acgtggtgga tcccgccggg cggctgcggt cggagagggc gtgccatccc gcccaccgta
     5341 cggccgggcg cctgccgcgc aagggcgcgc gcgagctttg gcacggtttc gtcacgcttt
     5401 cggtacgggg tctgaccccg taagcccgtc cgccgcgcga cctccgtcac acctggcggc
     5461 cacggaaaac cgtcgttttc cagggatgtc gcgctcccgt cacggacgcg tgcggctccc
     5521 gtcacggggt ctgaccccgt aagcgcgggc gcgacacact tccgtgacac gttccggccc
     5581 tcggaaagcg cgctgcgagc agggacgtca cgaagtcggc acacagtcgt gtgcggtttg
     5641 gtgcagggtc tgaccccctg acggctcgcg acccgctacg cgcgcgcgac ggcgaggtcg
     5701 tcgccgggct ccacgcggac ggcgagggtc tcgcgcgcga tccagtcggt gtggtccgcg
     5761 agggcgtcgt ggtcgtcggc cggaagcgtg agcgtgatcc ggtcggtcag ctccaggccg
     5821 gcctccttcc gcatcccgtt cacccggtgg atgaggtcgt ggacgcggcc ctcgaggcgt
     5881 agctcgtcgt cgagcgccgt gtcgagcgac accgtgatcc cgtcgtccgc cgccaccgcg
     5941 aacccctcgc ggccg
//
LOCUS       DASTFC010000019         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_313406,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000019 DASTFC010000000
VERSION     DASTFC010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313406"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFO88_00555"
     CDS             complement(<1..180)
                     /locus_tag="VFO88_00555"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET9544042.1"
                     /translation="MNAALAAAAFAPALAIGSFLNVVVARLPEKRSLVRPRSACPACG
                     HELAWWENVPLLSYAL"
     gene            complement(186..1172)
                     /locus_tag="VFO88_00560"
     CDS             complement(186..1172)
                     /locus_tag="VFO88_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544043.1"
                     /translation="MRGRVAAAWALVLAAAGAVGWRDRAADAGDLGFFVSAGRTLVSG
                     DWASTFADPAVQAGPLGLGPLGLAARLADTLGVSTLGLLSVGIQVAGAAAILAVATLV
                     LRGRAWWLPAAVGAAAVALGLPSSAFVDGHPAQLFVPLLWVCAAHLALRGNVAWAGAL
                     VGLSAGLETWGALGVAALALAPRARGALTGSAVAAAAAAALYLPFALAGEFRMLEYRW
                     TVAEGTLPSLWLEQGGAFPWELRLLQGAAAVGAGVVVALLVRRTSHAAWAPLLAVVAV
                     RLLLDPLGYSWYWLALQALMLVGAAELLSSPAFADLRRRISGPRAGVPMETG"
     gene            complement(1173..1448)
                     /locus_tag="VFO88_00565"
     CDS             complement(1173..1448)
                     /locus_tag="VFO88_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544044.1"
                     /translation="MTPEANAAAAAPGEEIELDVRTVRKDGKQVVTLRAVQRPLECSV
                     ECEVYPVSGLRVDPLNPGPYTFATPGEATAFVAEAVEALTILGCQES"
     gene            complement(1451..2299)
                     /locus_tag="VFO88_00570"
     CDS             complement(1451..2299)
                     /locus_tag="VFO88_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544045.1"
                     /translation="MDGLRSNLRPRVLLPTAILALGGIGFGAFAFSGTPGAGDEPLPP
                     IVQPAKPAEEPSASLAAWRKDANGVCASLNHDTANLGTPQTQAELLQRLPASLTLAEG
                     ALKELEALPTPKKQRKQIEKMLKLFGRFVAQQQAALTALQAADTAEFARLTGLAFQAN
                     DRGNAIARDLGARRCAVGGTDDTKLTRELERKRVVVAVLYAPDSNVDTLAIREARAGA
                     LLAGAGFVAVNVYDPQAIAPLTARYSVRTSPSVFVFARSKGAVSQFGGYVDRETVAQA
                     ADTAAL"
BASE COUNT          351 a          903 c         1001 g          282 t
ORIGIN      
        1 gagcgcgtac gagaggagcg gcacgttctc ccaccaggcg agctcgtgac cgcaggccgg
       61 gcaggccgag cgaggccgaa caagcgagcg cttctccggc aggcgcgcga cgacgacgtt
      121 caggaagctc ccgatcgcga gcgcgggggc gaaggcggcg gcggcgaggg cggcgttcac
      181 ggaggtcacc ccgtctccat cggcacgccg gcgcgcgggc ctgagatccg gcggcgcagg
      241 tcggcgaacg ccggcgagct gagcagctcg gccgcgccga cgagcatgag cgcctggagg
      301 gcgagccagt accaggagta gccgagcggg tcgaggagca gccgcacggc gacgaccgcg
      361 aggagcggcg cccaggccgc gtggctcgtc ctgcgcacga gcagcgcgac gacgacaccg
      421 gcgccgaccg cggccgcgcc ctgcaggagc cggagctccc aggggaacgc gccgccctgc
      481 tcgagccaga ggctcgggag cgtgccctcg gcgaccgtcc agcggtactc gagcatgcgg
      541 aactcgccgg cgagggcgaa cgggaggtac agcgcggcgg cggccgccgc agcgacggcg
      601 ctccccgtga gcgccccccg cgcgcgcggt gcgagcgcga gcgcggcgac gcccagcgcg
      661 ccccacgtct cgagcccggc ggagagcccg acgagcgcgc ccgcccaggc gacgttgccg
      721 cgcagcgcga ggtgcgcggc gcagacccag aggagcggca cgaagagctg agccggatgc
      781 ccgtccacga acgcggagct cggcagcccg agcgcgaccg cggccgcgcc gaccgcggcg
      841 gggagccacc atgcgcggcc ccgcaggacg agcgtcgcga ccgcgaggat cgccgcggcg
      901 ccggcgacct ggattccgac ggagagcagc ccgagcgtcg agacgccgag cgtgtccgcg
      961 aggcgggccg cgagcccgag cggcccgagc ccgagcggcc ccgcctgcac ggcggggtcg
     1021 gcgaacgtcg acgcccagtc gcccgagacg agcgtccggc cggccgacac gaagaacccg
     1081 aggtcgcccg cgtccgcggc gcggtcacgc cagccgacgg ctccggcggc ggcgaggacg
     1141 agcgcccagg ccgcggcgac gcgcccgcgc actcaggact cctggcagcc gaggatcgtg
     1201 agcgcctcca cggcctcggc cacgaacgcg gtcgcctcgc ccggcgtcgc gaaggtgtag
     1261 gggcccgggt tcagcgggtc gacgcgcagg ccggagacgg ggtagacctc gcactcgacc
     1321 gagcactcga gcgggcgctg caccgcgcgg agcgtgacga cctgcttccc gtccttccgg
     1381 acggtgcgca cgtcgagctc gatctcctcg ccgggcgccg cggccgcggc gttcgcctcc
     1441 ggcgtcatcg ctagagagcc gcggtgtcgg ccgcctgcgc cacggtctcg cggtcgacgt
     1501 agccgccgaa ctgggagacc gcgcccttcg agcgggcgaa gacgaagacg gagggcgacg
     1561 tccgcacgct gtaccgggcc gtgagcgggg cgatcgcctg cgggtcgtag acgttcaccg
     1621 cgacgaagcc tgcgccggcg aggagcgcgc ccgcgcgcgc ctcgcggatc gcgagcgtgt
     1681 cgacgttcga gtccggcgcg tagaggacgg cgacgacgac gcgcttgcgc tcgagctcgc
     1741 gcgtgagctt cgtgtcgtcg gtgccgccga ccgcacaccg acgggcgccg aggtcgcggg
     1801 cgatcgcgtt gccgcggtcg ttcgcctgga acgcgagccc ggtgaggcgt gcgaactcgg
     1861 ccgtgtcggc cgcctggagc gcggtcagcg ccgcctgctg ctgtgcgacg aaccgcccga
     1921 agagcttcag catcttctcg atctgcttgc gctgcttctt cggcgtcggc agggcctcga
     1981 gctccttcag cgctccctcg gccagcgtga gcgaggcggg gaggcgctgg agcagctcgg
     2041 cctgcgtctg cggcgtgccg aggttcgcgg tgtcgtggtt cagggaggcg cagacgccgt
     2101 tcgcgtcctt gcgccatgcg gcgagcgacg cggagggctc ctcagcgggc ttcgccggct
     2161 ggacgatcgg cggcagcggc tcgtcgcccg cgcccggcgt gccggagaac gcgaacgccc
     2221 cgaagccgat cccgccgagc gcgaggatcg cggtcgggag gagcacgcgg ggccgcaggt
     2281 tcgaccgcag tccgtccatg gtgtctctat cggcaggagg aggcgcgcct tgagatcagg
     2341 tccacgcgcg cgcggttaac gacgaggggc gcggtctccc gcgcccctcg tcgtttcggt
     2401 tccggtgtcc gcttaggagc aggcgccggt gacgatgtcc gccgaggggc cgttcttgtt
     2461 gaaggtggcc tggccgacgg tcgactgcac gcagtaggtg gtggcgttga gcgtggagcc
     2521 gaaggtgatg ttcttga
//
LOCUS       DASTFC010000020         6549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_379268,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000020 DASTFC010000000
VERSION     DASTFC010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379268"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFO88_00575"
     CDS             <1..223
                     /locus_tag="VFO88_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544046.1"
                     /translation="FFALLAADRLGDVWASVRDLPLVVEGILWLAFLPWLLGTAVWES
                     SWPDALRVALVVLFAAGWTLASIPRAKHP"
     gene            310..585
                     /locus_tag="VFO88_00580"
     CDS             310..585
                     /locus_tag="VFO88_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544047.1"
                     /translation="MTTAPLPIPATRREPRAWFVSRPFWTSIAIVAMWTAVIVDAIVG
                     TEIVAVATTATTTTTTNIPTVVITGLLAAIATIFVARYGFSPERRDG"
     gene            complement(592..1902)
                     /locus_tag="VFO88_00585"
     CDS             complement(592..1902)
                     /locus_tag="VFO88_00585"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET9544048.1"
                     /translation="MAATVVLLNRVLRVRDHPALASAAREGDAVVPLFVFDPAILRTF
                     GAPNRVAFLLDALADLDRSLERQGAGLVVRRGDAAEEARRVAADVGAATIHASADASG
                     TARRREERLDAELHPGVFVVPPGVLAPDGSDHYRVFTPYWNRWREHATRTVEEAPTGL
                     RLPDGLDRGRLPSLGELVPGDPSPALPRGGETAGRERLLAWGGPSGYGERRDDLAADA
                     TSRLSPYLHLGCLSPLELAAQVAGRPDAEPFLRQLAWRDFAGQLFAANPRLPRENLRA
                     GRHEWNDDPDGLAAWKEGRTGYPLVDAGMRQLRAEGWMPNRARLVTASFLVKDLGIDW
                     RAGAAHFEALLVDGDLASNRVNWQWVAGTGVDPRPERILNPTRQAQRFDPDGAYVRRW
                     IPELAGISGGAVHEPWKLGLEAPADYPPPLVDHLEAVRRLRGRR"
     gene            complement(1902..2594)
                     /locus_tag="VFO88_00590"
     CDS             complement(1902..2594)
                     /locus_tag="VFO88_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HET9544049.1"
                     /translation="MRVHVAFTPAEHAEAPTGIVVDVLRATSTIAEALASGHARVLCC
                     SEIEQAEALRAALGDGLLAGERKCLRIPGFDRGNSPSEFTTGGGETVILTTTNGTRAL
                     VAAAARCERVYGASLLNLDAVVAAARDAGDDVAVFCAGVKGEFAMDDAYVAGRLVEEL
                     GGDGTDAATAAVRLRASFASAEDGLGASQSARNLVAAGLEEDIAWCARESVLDVVPRV
                     DGSDGPAVVLRT"
     gene            2632..3486
                     /locus_tag="VFO88_00595"
     CDS             2632..3486
                     /locus_tag="VFO88_00595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9544050.1"
                     /translation="MASTEQAMVCYRHPGRETLVSCSNCDRPICTDCMVQTAVGIKCP
                     ECAGQPTGVARAARSAGRAVGEGAGARVTKALIAVNVGIFLLQYATGSGRPLESELFE
                     RGALYGPLVADGEWWRLVTAGFLHASVIHIAFNMLMLWWFGSALEGLLGRGRFLAVYG
                     LGLLTGSAGALLLSPEAATVGASGAVFAVLGAGLVLERRGINVFGGAALTVVLLNVAI
                     TLFVPNISLGGHLGGLAGGILVILALMHFSRSHPAYSKIDVTTVASLLAIGVGALLLS
                     YLRVRGYA"
     gene            3549..3932
                     /locus_tag="VFO88_00600"
     CDS             3549..3932
                     /locus_tag="VFO88_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET9544051.1"
                     /translation="MTMQNDMWTYRQESWTRGGTAVTDTTSGIDLTGLSVEAIDGGIG
                     KVDEATHEVGSSYIVVDTGPWIFGKKVMLPAGVVESVDLDSETVYVNRSKDEIKNAPE
                     FDETRIEDTRYRDELGSYYGGRSGH"
     gene            complement(3987..>6549)
                     /gene="ppdK"
                     /locus_tag="VFO88_00605"
     CDS             complement(3987..>6549)
                     /gene="ppdK"
                     /locus_tag="VFO88_00605"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET9544052.1"
                     /translation="LLGGKGAGLAEMTALGVPVPGGFVVSTQACVEYMRAGREPDGLA
                     EQVDEAIGRLEEATGKRFGDPHDPLLVSVRSGAATSMPGMMDTILNLGLSDVAVDGLV
                     ASTGNPRFAYDAYRRLVQMYGDVVSGVSGQRFEDELDRMKGERGAAQDVDLTADDLRE
                     LVERFKAIYREGTGEDFPQDARDQLGRAIRAVFDSWDVPRAQVYRRAHGIPDDLGTAV
                     NVVQMVFGNKGDTSGTGVCFTRDPSTGEPGIWGEFLVNAQGEDVVAGIRTPEPLDRMK
                     DALPQAYEQLVETLRSLEAHYREMQDIEFTVEDGRLYILQTRTGKRTAVAALRIVVDM
                     VGEGLISREEAIARIEAGQLDQLLHPMIDPRAEYEPAAKGLNASPGAAKGAIVLDADT
                     AEERGRAGEDVILVRRETTPDDIHGLIQARGVLTAHGGMTSHAAVVARGMGKPCVAGC
                     EGLSIDLGARTVTIGGRELAEGDRITIDGGSGHVILEDVELVPPQINEHFETILGWAD
                     EVRRLRVRANADTPEDAAKAREFGAEGIGLCRTEHMFMAEDRLPVVRAMILASNEEER
                     REALDRILPFQQSDFEGIFEAMAGLPVTIRLLDPPLHEFLPPLEEADSPQMAARIRQL
                     QEANPMLGTRGCRLGLMFPEIYEMQVRAIIRAALAVEERTGDAPLIEIMHPLVGFAEE
                     LRRLRGLTLDTASAELEQASREIDYLVGTMIELPRACLRSDEIAEHADFFSFGTNDLT
                     QTTLGFSRDDAENGFLVHYLEEDVLPENPFQTLDRSGVGDLMKIAIERARAVKPDLKL
                     GICGEHGGDPRSVAFCHDLGLAYVSCSPYRVPLARLAAAQAALSEAGVTAVAVGG"
BASE COUNT          861 a         2376 c         2399 g          913 t
ORIGIN      
        1 gttcttcgcg ctcctcgccg cagaccggct cggcgacgtc tgggcgtccg tgcgagacct
       61 gccgctcgtc gtcgagggga tcctctggct cgcgttcctc ccctggctgc tcggaacggc
      121 cgtctgggag agctcgtggc ccgacgcgct gcgggtcgcc ctcgtcgtgc tcttcgcggc
      181 gggctggacg ctcgcctcga taccgcgcgc gaagcatccg tagttccggc gcgaagatcg
      241 ggcgccaccc cgatgccccg gcggcgcgcc ggagggacgg ttcggatgca ggccagcgaa
      301 ggaggagtca tgacaaccgc accgcttccc atcccggcaa cccgccggga gccgcgcgca
      361 tggttcgtgt cgcgtccgtt ctggacgtcg atcgcgatcg tcgcgatgtg gactgccgtg
      421 atcgtcgatg cgatcgtcgg gaccgagatc gtggccgtgg cgacgaccgc gacgacgacc
      481 acgacgacga acatcccgac cgtcgtgatc acggggctgc tcgcggcgat cgcgacgatc
      541 ttcgtcgcgc gctacggctt ctcgcccgag cggcgcgacg gatagcggcc gctaccggcg
      601 cccgcgcagg cggcggacgg cctcgaggtg gtcgacgagc ggcggcggat agtccgccgg
      661 cgcctcgagc cccagcttcc agggctcgtg gaccgcaccg ccggagatcc cggcgagctc
      721 cgggatccag cggcggacgt acgcgccgtc cgggtcgaac cgctgcgcct ggcgcgtcgg
      781 attgaggatc cgctccggcc gcgggtcgac gcccgtgccc gcgacccact gccagttgac
      841 gcggttgctc gcgaggtcgc cgtccacgag cagggcctcg aagtgcgccg cgccggcgcg
      901 ccagtcgatc ccgagatcct tcacgaggaa ggacgcggtc acgagccgcg cccggttcgg
      961 catccacccc tccgcgcgga gctggcgcat gcccgcgtcg acgagcggat agccggtgcg
     1021 gccctccttc cacgcggcga ggccgtccgg gtcgtcgttc cactcgtggc ggcccgcgcg
     1081 gaggttctcc cgcggcaggc gcgggttcgc ggcgaaaagc tgtccggcga agtcgcgcca
     1141 cgcgagctgg cgcaggaacg gctcggcgtc cggccgcccg gcgacctgag ccgcgagctc
     1201 gagcggggag aggcagccga ggtggaggta cgggctgagt ctcgaggtcg cgtccgcggc
     1261 gaggtcgtcg cggcgctcgc cgtagcccga agggccgccc cacgcgagga gccgctcgcg
     1321 gccggccgtc tccccgcccc gtgggagcgc gggcgacggg tcgcccggga cgagctcgcc
     1381 gagcgaaggc agccgcccgc ggtcgagccc gtcggggaga cgcaaccccg taggggcctc
     1441 ctcgacggtc cgcgtcgcgt gctcccgcca gcggttccag tagggcgtga agacgcggta
     1501 gtggtcggag ccgtccggcg cgagcacgcc gggcgggacg acgaacacgc cgggatggag
     1561 ctcggcgtcg agccgctcct cccgccgccg cgccgtgccg ctcgcgtccg cgctcgcgtg
     1621 aatcgtcgcc gcaccgacat ccgccgcgac gcggcgcgcc tcctcggccg cgtcgccgcg
     1681 gcgcacgacg agcccggccc cctgacgctc gagcgagcga tcgaggtcgg cgagcgcatc
     1741 gaggaggaac gcgacccggt tcggcgcccc gaacgtccgc aggatggccg ggtcgaagac
     1801 gaagagcggc acgaccgcgt cgccctcgcg cgccgcggag gcgagcgcgg gatggtcgcg
     1861 cacgcggagc acgcggttca ggaggacgac ggtcgcggcc actacgtccg cagcacgacg
     1921 gcgggcccgt cggacccgtc gacccgcgga acgacgtcga gcacgctctc gcgcgcgcac
     1981 cacgcgatgt cctcctcgag cccggccgcg acgaggttgc gggcgctctg gctcgcgccc
     2041 agcccgtcct ccgccgacgc gaacgaggcc cgtagccgca cggccgccgt tgccgcgtcc
     2101 gtcccgtcgc cgccgagctc ctcgacgagc cggccggcca cgtacgcgtc gtccatggcg
     2161 aactcgccct tcacgcccgc gcagaagacc gccacgtcgt cgcccgcgtc gcgcgccgcc
     2221 gcgacgacgg cgtcgaggtt gaggagcgat gccccgtaga cccgctcgca gcgcgcggcc
     2281 gcggcgacga gcgcgcgcgt gccgttcgtc gtcgtcagga tgaccgtctc gccgccgccg
     2341 gtcgtgaact cggacggcga gtttccgcgg tcgaagccgg ggatccggag gcacttccgc
     2401 tcgccggcga ggagcccgtc gccgagcgcg gcgcgcagcg cctctgcctg ctcgatctcg
     2461 gagcagcaga ggacgcgcgc gtggccggac gcgagcgcct cggcgatcgt ggacgtcgcg
     2521 cggaggacgt cgacgacgat cccggtcggc gcctcggcgt gctcggccgg ggtgaaggcg
     2581 acgtggacgc gcacggcgcg tgacgttaca gggcgccgtt acgatcggcg gatggcatcg
     2641 accgagcagg cgatggtgtg ctaccgccat ccgggccgcg agacgctcgt gtcctgctcg
     2701 aactgcgacc gtccgatctg cactgactgc atggtgcaga ccgccgtcgg gatcaagtgc
     2761 ccggagtgcg ccggacagcc gaccggcgtc gcgcgcgcgg cccggagcgc cgggcgcgcg
     2821 gtcggggagg gcgcgggcgc gagggtcacg aaggccctca tcgcggtgaa cgtcgggatc
     2881 ttcctcctcc agtacgcgac cgggagcggg cgtccgctcg agagcgagct cttcgagcgc
     2941 ggcgcgctgt acgggccgct cgtcgcggac ggcgagtggt ggcgcctcgt cacggcgggg
     3001 ttcctgcacg cgagcgtgat ccacatcgcg ttcaacatgc tcatgctctg gtggttcggc
     3061 tcggcgctcg agggcctgct cggccgcggg cgcttcctcg ccgtgtacgg gctcgggctg
     3121 ctcacggggt cggccggcgc gctgctcctg agccccgagg cggcgacggt cggggcgtcg
     3181 ggcgcggtgt tcgccgtcct cggcgccggc ctcgtcctcg agcggcgcgg gatcaacgtc
     3241 ttcggcggcg ccgcgctcac cgtggtgctc ctgaacgtcg cgatcacgct gttcgtcccg
     3301 aacatctcgc tcggcggcca tctcggcggc ctcgcgggcg gcatcctcgt catcctcgcg
     3361 ctcatgcact tctcgcgcag ccacccggcc tactcgaaga tcgatgtgac gaccgttgca
     3421 agcctcctgg cgatcggcgt cggcgcgctc ctcctctcgt atctacgggt acgaggctac
     3481 gcataagcgg gttcaggacg agccgcaggg gtacgccccg ggggacaccg aaatcgaggg
     3541 ggtaccagat gaccatgcag aacgacatgt ggacgtaccg tcaggagtcc tggacgcgcg
     3601 gcggcaccgc cgtgacggac acgacgtcgg ggatcgacct gaccgggctg agcgtcgagg
     3661 cgatcgacgg cggcatcggc aaggtcgacg aggccacgca cgaggtcggc tccagctaca
     3721 tcgtcgtcga caccggcccg tggatcttcg gcaagaaggt catgctcccg gccggggtcg
     3781 tcgagtcggt cgacctcgac agcgagacgg tgtacgtgaa ccgctcgaag gacgagatca
     3841 agaacgcgcc ggagttcgac gagacccgca tcgaggacac gcggtatcgc gacgagctcg
     3901 gctcgtacta cggcggccgc tcgggtcact gaccgagcgt tcgaccggcg tttcgaaggg
     3961 cggcccacgg gccgcccttc gcgtacctag ccgccgaccg cgacggccgt cacgccggcc
     4021 tcgctgagcg ccgcctgggc cgcggcgaga cgggcgagcg ggacgcggta gggcgagcag
     4081 ctcacgtacg cgagcccgag gtcgtggcag aaggcgaccg acctcgggtc gccgccgtgc
     4141 tcgccgcaga tcccgagctt caggtcgggc ttcacggcgc gggcccgctc gatcgcgatc
     4201 ttcatgaggt cgccgacccc gctccggtcg agggtctgga aggggttctc cggcagcacg
     4261 tcctcttcga ggtagtggac gaggaagccg ttctccgcgt cgtcgcgcga gaacccgagc
     4321 gtcgtctgcg tcaggtcgtt cgtcccgaag gagaagaagt ccgcgtgctc ggcgatctcg
     4381 tccgagcgca ggcacgcacg cgggagctcg atcatcgtcc cgaccaggta atcgatctcg
     4441 cgggaggcct gctcgagctc cgccgaggcc gtgtcgagcg tcagcccgcg gaggcggcgc
     4501 agctcctcgg cgaagccgac gagcgggtgc atgatctcga tgagcggcgc gtcgccggtc
     4561 cgctcctcga ccgcgagcgc ggcccggatg atcgcccgga cctgcatctc gtagatctcc
     4621 gggaacatga gcccgagccg gcagccgcgc gtgccgagca tcgggttcgc ctcctggagc
     4681 tggcggatcc gcgcggccat ctgcggcgag tcggcctcct cgagcggcgg gaggaactcg
     4741 tggagcggcg ggtcgagcag ccggatcgtg acggggagcc cggccatcgc ctcgaagatc
     4801 ccctcgaagt cgctctgctg gaacggcagg atccggtcga gcgcctcgcg ccgctcctcc
     4861 tcgttcgagg cgaggatcat cgcgcggacg acgggcaggc ggtcctcggc catgaacatg
     4921 tgctccgtcc ggcagaggcc gatcccctcc gcgccgaact cgcgcgcctt ggccgcgtcc
     4981 tcaggcgtgt cggcgttcgc gcgcacgcgc aggcggcgca cctcgtccgc ccagccgagg
     5041 atcgtctcga agtgctcgtt gatctgcggc gggacgagct cgacgtcctc gaggatcacg
     5101 tgcccggagc cgccgtcgat cgtgatcctg tcgccctcgg cgagctcgcg gccgccgatc
     5161 gtgaccgtcc gcgcgccgag gtcgatcgag agcccctcgc agccggcgac gcacggcttc
     5221 cccatgcccc gcgcgacgac ggccgcgtga gacgtcatcc cgccgtgcgc ggtcaggacg
     5281 ccccgggcct ggatcagccc gtggatgtcg tcgggcgtcg tctcgcggcg gacgaggatg
     5341 acgtcctccc cggcccggcc gcgctcctcg gcggtgtcgg cgtcgaggac gatcgctccc
     5401 ttcgccgctc cgggcgaggc gttcaggccc ttcgcggccg gctcgtactc cgcgcgcggg
     5461 tcgatcatcg ggtggaggag ctggtcgagc tggccggcct cgatgcgcgc gatcgcctcc
     5521 tcccgcgaga tgaggccctc gccgaccatg tccacgacga tccggagcgc agcgaccgcc
     5581 gtccgcttgc ccgtgcgcgt ctggaggatg tagagacggc cgtcctcgac ggtgaactcg
     5641 atgtcctgca tctcgcggta gtgcgcctcg aggctgcgaa gcgtctcgac gagctgctcg
     5701 tatgcctgcg ggagcgcatc cttcatccgg tcgagcggct cgggcgtgcg gatgcccgcg
     5761 acgacgtcct cgccctgcgc gttcacgagg aactcgcccc agatgcccgg ctccccggtg
     5821 gaggggtcgc gcgtgaagca gacgccggtg ccggaggtgt cgcccttgtt cccgaacacc
     5881 atctggacga cgttcaccgc ggtgccgagg tcgtcgggga tcccgtgcgc gcgccggtag
     5941 acctgggcgc gcgggacgtc ccaggagtcg aacacggcgc ggatcgcgcg gccgagctgg
     6001 tcgcgcgcgt cctgcgggaa gtcctcgccg gtgccctcgc ggtagatcgc cttgaagcgc
     6061 tccacgagct cccggaggtc gtccgcggtc aggtcgacgt cctgcgcggc tccacgctcg
     6121 cccttcatcc ggtcgagctc gtcctcgaac cgctggccgc tcacgccgga gaccacgtcc
     6181 ccgtacatct ggacgaggcg gcggtacgcg tcgtaggcga agcgcgggtt cccggtcgac
     6241 gcgacgagcc cgtcgacggc gacgtcgctc aggccgaggt tcaggatcgt gtccatcatc
     6301 ccgggcatcg aggtcgcggc gccggagcgg accgagacga gcagcggatc gtgcgggtcg
     6361 ccgaagcgct tccccgttgc ctcctccagc cggccgatcg cctcgtccac ctgctcggcg
     6421 agcccgtccg gctcgcgccc ggcgcgcatg tactcgacgc acgcctgcgt cgagacgacg
     6481 aatccgcccg gcacggggac gccgagcgcg gtcatctccg cgaggccggc gcccttgccg
     6541 ccgaggagc
//
LOCUS       DASTFC010000021         7444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_395856,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000021 DASTFC010000000
VERSION     DASTFC010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395856"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFO88_00610"
     CDS             complement(<1..339)
                     /locus_tag="VFO88_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544053.1"
                     /translation="MRRLKGRRPALVAALLALAVAVPLAAWAAPRYDTPDRPHSKNMH
                     LIGASLRAGAVTGPPPAGPGTVPWDTRNTDLAFWGDLAIQGRYDGFRVIDIKAPGNPR
                     ELALFTCVSPQ"
     gene            571..1314
                     /locus_tag="VFO88_00615"
     CDS             571..1314
                     /locus_tag="VFO88_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544054.1"
                     /translation="MRSRLTRRGRRTAAAGLALAAAVLVSASPAAARPSTPPRVEIST
                     HPALLAPDGRSVGVVVVARCADRWSVVEASVTVTQPQASGRGTIPLTCIGFDRVFHVL
                     VPVSSGTFALGSASVTATVVASRGKTQRADDSATLPVDPTVFVDLDGSARLEPGGSAV
                     RLGLTVACPVGATGVPSRVGVFQGQTSGFGPYTPVCDGAEHRFDVLVNSTNGLFAAGI
                     AQALTFADITYDGRTFSGIDDDGALEIVP"
     gene            1414..2976
                     /locus_tag="VFO88_00620"
     CDS             1414..2976
                     /locus_tag="VFO88_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HET9544055.1"
                     /translation="MEHHGSESYFVLGEGLLTESVGGREPTLARTLETSAPPFRFSRM
                     GPKGAQLTDATRRKLGFAMTEGGGGAGTIPAGYTYLGQFVDHDLTFDKTAVMLGESVA
                     PSQLLQARSPSLDLDSLYGAGPGDPGSAKFYKPDGIRLKTGATEAIGPDKAKKDFDLP
                     RGAGSSRKQKRRAIIPDPRNDENLAVAQTHLALIRFHNRVAQSLPGPLTPAQRFAKAR
                     ELVVKHYQWMLRTDYLPRICAPAVVRDVFKNGRKAFEVGAPPTSVPTMPIEFSVAAFR
                     LGHSMIRGTYDWNARFPDKDGFLFFLFDFSAVSGNLGGGLRLPSNWIADWRRLYDFKE
                     AGRNDLAHRVNRAMRIDTTLARPLHSLPTGSFGGPEVDFDDPRADLAFRNLTRAKMVK
                     LATGQKMASFLRSKGVDVKTLTPAQIREGKGGAQLDSLTSAQRKSVVQATPLWFYVLR
                     EAELGGGKLRGVGARIVAETFHRAMEGSEHSIVRDKTWKPTLGPDSKTFRMVDLLLFA
                     FEGKKSLLAPLA"
     gene            complement(2989..3570)
                     /locus_tag="VFO88_00625"
     CDS             complement(2989..3570)
                     /locus_tag="VFO88_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544056.1"
                     /translation="MRRLFVIGGIVSGVLLILFGLGSIWISANGYTDVRDSLERENIV
                     GSPDMEPEATRVAVEESGAEIDDLPDCDVAGEEVTTGAEARCFADYMRIHTLEATDGQ
                     TYAEMGRFLDEEGNATSEESEAAVGENGRPVENPQRQLWVTETALATALNMAFLAENL
                     AVFGVVVGIALFLAGIGFIVLALGALRLRDDRY"
     gene            3716..4225
                     /locus_tag="VFO88_00630"
     CDS             3716..4225
                     /locus_tag="VFO88_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544057.1"
                     /translation="MVANVIPTRVHGIIDYTTSGALLAAPEVFRLKDVRSSSLAPRIA
                     GAAASLYAPLTDYELGAVKALPMKAHLALDAAGGALLAASPWVLGYRRHGVRHWLPHT
                     LVGLGEVAVALTSKTENGKESRRRRFGKTALISSAVVVPAAAGAALLARRRSRNGGSS
                     ATEGAGDES"
     gene            complement(4248..5489)
                     /locus_tag="VFO88_00635"
     CDS             complement(4248..5489)
                     /locus_tag="VFO88_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544058.1"
                     /translation="MAERHLIGLLLGAEEDWPSVFEELAARVGPITWRGERHELATER
                     ISIGPFDLRAKPRHALVIDRLAHWYYVPREWLKKVALMDDVYLLNNPFTFQSMEKHAA
                     YCGMLRLGLKVPETWLIPHKRPPVDDRFSYTASKYNTPFDLPEIARGIGYPLYMKPFD
                     GGAWVGVTRIANEAELVARYEESGRRLMHLQAAVEFDVFARSLTIGAETLVVRFDPGR
                     PMYDRYQVEHGFLAPETGWEVETIGRLVNAFFRWEFNSCETLVRGDEVFPIDYANASP
                     DVSITSLHYYFPWAMRTLLKWCAFCTVTRRAMNVNQDSRPYHAFADRDDLAYDEKLAS
                     YRALAEEYFQTEEYEEFCAEALPHVDELVVDYVESRAFDDLLVRTVTGTFPAHEHERF
                     VPHYRGLLEAWVGDQRAAATA"
     gene            complement(5489..6235)
                     /locus_tag="VFO88_00640"
     CDS             complement(5489..6235)
                     /locus_tag="VFO88_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9544059.1"
                     /translation="MGVGRRELELHSPAIGAAGRILAYGHWGRPLLAFPSEQGSAADW
                     EDRGMVDAIAPLLEAGRVKLYCVDSYDAQSWHAHGLSLEDRARRHEAFEDWIANQVAP
                     FVRADSGDAADVAVAGTSFGAYHAANAALRRADLFPLALCFSGVYDVSIVGDGERGDA
                     FYFQNPMDYVRNLHGDHLDWLRSRVSVLLVCGQGMWEDTTGALESTKAFGGLLAERGI
                     PVEVDLWGHDVPHDWPSWRAQLAHHLPRFC"
     gene            complement(6226..7308)
                     /locus_tag="VFO88_00645"
     CDS             complement(6226..7308)
                     /locus_tag="VFO88_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9544060.1"
                     /translation="MVARPHAGAAPVADEVAFSLADPEGRLRRVRLVHELWRPRVGPD
                     FARAGRGRPWTLSIPRPPVDRIEYRLEVTDAEGGVETVCDPGNPLRAPGPFGEKSVLE
                     LPGYRPPAWLDADADAPAGRREILTIESRTLHASFDVGLWTPAGVRGGRPLPLLLAHD
                     GPEYADYSALLRFAAAAVAAGEVPPFRVALVPPRERNRDYSASARYGRALVSELLPAI
                     ERAAPTPGGPEARVALGASLGALALVHAHRLRRGLFGGLFLQSGSFFRQRFDRYERSF
                     PHFDRISRFVGSVLGAAEWPDPVPAVLTCGTVEENLANNRAVASALASQGYDVRLEVV
                     RDAHNWIGWRDALAPHLPGLLRRLWE"
BASE COUNT         1048 a         2691 c         2725 g          980 t
ORIGIN      
        1 ctgcgggctc acgcacgtga agagcgcgag ctccctcggg ttgccgggcg ccttgatgtc
       61 gatcaccctg aagccgtcgt agcggccctg gatcgcgagg tcgccccaga aggcgaggtc
      121 ggtgttccgc gtgtcccacg gaaccgtgcc cggcccggcg ggcggcgggc ccgtgacggc
      181 gccggcccgc aggctggcgc cgatcaggtg catgttcttg ctgtgcggcc ggtcgggcgt
      241 gtcgtatcgc ggggcggccc aggccgcgag cggcacggcc accgcgagcg cgaggagcgc
      301 ggcgacgagc gccggccttc gtcccttgag tctccgcatt cggtgagctc cttccgaatc
      361 gggaggcgac gcgtcccgca cgcccggccg caagcgagcc cttggggcga tcgtcgcccc
      421 ggtgagcgca gtgaacggcg cggatgctac gacgacgcag gaggccttgt caaaagtcaa
      481 ttttcaccag gttgaaccgg cgccgcgcgg cgcacgtact cctggtgcag gcgggagagc
      541 cacaccgtcc agatccgaag gaggaagccc atgagaagcc gtctcacgcg gcgtggacgc
      601 cgtacggccg cggccggcct cgccctcgcc gccgccgtgc tcgtctccgc gtcgcccgcc
      661 gcggcgcggc cgtcgacccc gccgagggtc gagatctcca cgcaccccgc cctgctcgcc
      721 ccggacgggc gctcggtcgg cgtggtcgtc gtcgcccgct gcgcggatcg ctggagcgtg
      781 gtcgaggcgt ccgtcaccgt cacccagccg caggcgagcg gccgggggac gatcccgctc
      841 acctgcatcg gcttcgaccg cgtcttccac gtgctcgtgc cggtctcgag cgggacgttc
      901 gcgctcggga gcgcgagcgt gacggccacg gtcgtcgcct cacgcgggaa gacgcagcgc
      961 gcggacgact cggccacgct tccggtcgac ccgacggtgt tcgtcgatct cgacggctcg
     1021 gcccggctcg agccgggcgg gagcgccgtc cgcctcggcc tcacggtcgc gtgcccggtc
     1081 ggcgcgacgg gcgtgccgtc cagggtcggc gtcttccagg gccagacgtc gggcttcggc
     1141 ccgtacaccc ccgtctgcga cggcgcagag caccggttcg acgtcctggt caactcgacg
     1201 aacgggctct tcgccgccgg gatcgcccag gcgctcacgt tcgcggacat cacgtacgac
     1261 gggcggacgt tcagcggcat cgacgacgac ggggcgctcg agatcgttcc gtgacgcccg
     1321 ccggcgcggc gcggcggggg cagctcccgt cgcgccgccc ggcgagcggc tacagtcggc
     1381 acgtccggga gggcgccggc gagaggggga acgatggagc accacgggtc ggagagctat
     1441 ttcgtcctcg gcgagggact cctgacggag agcgtgggcg gccgcgagcc gacgctcgcg
     1501 cggacgctcg agacgtccgc cccgcccttc cgcttctcgc gcatgggccc gaagggcgcg
     1561 cagctgaccg acgcgacgcg gcgcaagctc ggcttcgcga tgacggaggg cggcggcggc
     1621 gcggggacga tcccggccgg ctacacgtac ctcgggcagt tcgtcgacca cgacctcacc
     1681 ttcgacaaga ccgccgtcat gctcggcgag agcgtcgcgc cgtcacagct cctgcaggcg
     1741 cgctccccga gcctcgacct cgactcgctc tacggcgccg gcccgggcga tcccggctcc
     1801 gcgaagtttt acaagccgga cgggatccgc ctgaagaccg gggcgaccga ggcgatcggc
     1861 ccggacaagg cgaagaagga cttcgacctg ccccgcggcg ccggctcctc gcggaagcag
     1921 aagcggcggg cgatcatccc cgacccgcgg aacgacgaga acctcgcggt cgcgcagacg
     1981 cacctggcgc tcatccgctt ccacaaccgc gtcgcgcaga gcctccccgg cccgctcacg
     2041 ccggcgcagc gcttcgccaa ggcgcgcgag ctcgtcgtca agcactacca gtggatgctg
     2101 cggacggact acctgccgcg gatctgcgcc cccgccgtcg tccgcgacgt cttcaagaac
     2161 ggccgcaagg cattcgaggt cggcgccccg ccgacgtccg tgccgacgat gccgatcgag
     2221 ttctccgtcg ccgcgttcag gctcgggcac tcgatgatcc gcggcacgta cgactggaac
     2281 gcccgcttcc cggacaagga cggcttcctc ttcttcctct tcgacttctc cgccgtgagc
     2341 gggaacctgg gcggcggcct ccgcctgccg agcaactgga tcgcggactg gcgccggctg
     2401 tacgacttca aggaggcggg ccgcaacgac ctcgcccacc gggtgaacag ggcgatgcgg
     2461 atcgacacga cgctcgcgcg gccgctgcac agcctcccga ccggctcgtt cggcgggccg
     2521 gaggtcgact tcgacgaccc gcgcgccgac ctcgcgttcc gcaacctcac gcgcgcgaag
     2581 atggtgaagc tcgccaccgg ccagaagatg gcctcgttcc tgcgctcgaa gggcgtcgac
     2641 gtgaagaccc tgaccccggc gcagatccgc gagggcaagg gcggcgcgca gctcgactcg
     2701 ctgacgagcg cgcagcggaa gtcggtcgtc caggcgacgc cgctctggtt ctacgtcctg
     2761 cgcgaggccg agctcggcgg cggaaagctc cgcggcgtcg gcgcgcggat cgtcgcggag
     2821 acgttccacc gcgcgatgga gggcagcgag cactcgatcg tccgcgacaa gacgtggaag
     2881 ccgacgctcg ggcccgactc gaagacgttc cggatggtcg acctgctcct cttcgccttc
     2941 gagggcaaga agagcctcct cgcgccgctc gcctagcggc ggcccgggtc agtaccggtc
     3001 gtcgcgcagg cgaagggcac cgagcgcgag cacgatgaag ccgattccgg ccaagaacag
     3061 cgcgatcccg acgaccaccc cgaacaccgc gaggttctcc gcgaggaacg ccatgttgag
     3121 cgccgtcgcg agcgcggtct ccgtcaccca gagctggcgc tgcgggttct cgaccggccg
     3181 gccgttctcg cccaccgcgg cctccgactc ctcggacgtc gcgttgccct cctcgtcgag
     3241 gaaccgcccc atctcggcgt acgtctggcc gtcggtcgcc tcgagcgtgt ggatccgcat
     3301 gtagtcggcg aaacagcgcg cctcggcgcc cgtcgtcacc tcctcgccgg cgacgtcgca
     3361 gtcgggcagg tcgtcgatct ccgcgcccga ctcctcgacg gcgacgcgcg tcgcctccgg
     3421 ctccatgtcg ggcgagccga cgatgttctc gcgctcgagg ctgtcgcgca cgtccgtgta
     3481 gccgttcgcg ctgatccaga tgctcccgag cccgaacagg atgaggagca ctcccgagac
     3541 gatgcccccg atgacgaaga gccgacgcat gagttcctcc ttggtcgaac gtcctggttg
     3601 aggctaccgc gcgcgcctcc cggcaatcgt cccaggcggt cgggtgtccg ggttcggggc
     3661 ccggagacgc gggtaggcgg accgccggac agagaccccg aggaggcaga cacgaatggt
     3721 tgcgaacgtc attcccaccc gtgtgcacgg gatcatcgac tacacgacga gcggcgcgct
     3781 cctcgcggcg ccggaggtct tccggctgaa ggacgtgcgc tcgtcctcgc tcgcaccgcg
     3841 catcgccggg gcggcagcgt cgctctatgc gccgctcacg gactacgagc tcggcgccgt
     3901 caaggcgctt ccgatgaagg cgcacctcgc gctcgacgcg gcgggcggcg cgctgctcgc
     3961 ggcctcgccg tgggtgctcg gctaccgccg ccacggcgtc cggcactggc tgccgcacac
     4021 gctcgtcggg ctcggcgagg tcgccgtcgc cctgacgagc aagacggaga acggcaagga
     4081 gagccggcgc cgccggttcg gcaagacggc tctcatctcg agcgccgtcg tggttccggc
     4141 cgcggccgga gcggccctgc tcgcgcggcg ccgctcccgc aacggcggct cgagcgccac
     4201 ggagggcgcc ggggacgagt cgtgagcggc gctccggagc ggcgcggcta ggccgtcgcg
     4261 gccgcccgct ggtcgcccac ccacgcctcg agcagcccgc ggtagtgcgg gacgaagcgc
     4321 tcgtgctcgt gcgcggggaa cgtgcccgtg accgtccgga cgagcaggtc gtcgaacgcg
     4381 cggctctcga cgtagtcgac gaccagctcg tccacgtgtg ggagcgcctc ggcgcagaac
     4441 tcctcgtact cctcggtctg gaagtactcc tcggcgagcg ccctgtacga cgcgagcttc
     4501 tcgtcgtagg cgaggtcgtc gcggtcggcg aacgcgtggt agggccgcga gtcctggttc
     4561 acgttcatcg cccgtcgcgt cacggtgcag aacgcgcacc acttgaggag cgtccgcatc
     4621 gcccacggga agtagtagtg gaggctcgtg atcgagacgt ccggcgacgc gttcgcgtag
     4681 tcgatcggga acacctcgtc gccgcgcacg agcgtctcgc acgagttgaa ctcccagcgg
     4741 aagaaggcgt tcacgagccg gccgatcgtc tccacctccc accccgtctc gggcgcgagg
     4801 aacccgtgct cgacctggta gcggtcgtac atcgggcggc ccgggtcgaa gcggacgacg
     4861 agcgtctcgg cgccgatcgt gaggctgcgg gcgaagacgt cgaactcgac ggccgcctgg
     4921 aggtgcatga ggcggcggcc cgactcctcg tagcgggcca cgagctccgc ctcgttcgcg
     4981 atcctcgtga cgccgaccca cgcgccgccg tcgaagggct tcatgtagag cgggtagccg
     5041 atcccgcgcg cgatctcggg caggtcgaag ggcgtgttgt acttcgaggc cgtgtaggag
     5101 aagcggtcgt ccaccggcgg gcgcttgtgc gggatgagcc acgtctccgg gaccttgagc
     5161 ccgagccgca gcatgccgca gtacgccgcg tgcttctcca tcgactggaa cgtgaacgga
     5221 ttgttgagga ggtagacgtc gtccatgagc gccaccttct tcagccattc gcgcgggacg
     5281 tagtaccagt gcgcgaggcg gtcgatcacg agcgcgtggc gcggcttcgc gcgcaggtcg
     5341 aacgggccga tcgagatccg ctcggtcgcg agctcgtggc gctcgccgcg ccaggtgatc
     5401 gggccgacgc gggcggcgag ctcctcgaag acgctcggcc agtcctcctc ggcgccgagc
     5461 agcaggccga tcaggtggcg ctccgccatc agcagaagcg cgggaggtgg tgggcgagct
     5521 gggcccgcca ggacggccag tcgtgcggga cgtcgtgccc ccagaggtcg acctcgacgg
     5581 gaatgccgcg ctcggcgagg aggccgccga atgccttcgt gctctcgagc gcgcccgtcg
     5641 tgtcctccca catcccctgc ccgcagacga gcagcacgct gacacggctt cggagccagt
     5701 ctaggtggtc gccgtgcagg ttgcgcacgt agtccatcgg gttctggaag tagaacgcgt
     5761 cgccgcgctc gccgtcgccc acgatcgaga cgtcgtagac gccgctgaag cagagcgcga
     5821 gcgggaagag gtcggcgcgg cgcagggcgg cgttcgcggc gtggtaggcg ccgaagctcg
     5881 tccccgcgac ggcgacgtcg gcggcgtcgc cgctgtcggc ccggacgaag ggggcgacct
     5941 ggttcgcgat ccagtcctcg aaggcctcgt ggcggcgcgc ccggtcctcc agggaaaggc
     6001 cgtgcgcgtg ccagctctgc gcgtcgtagg agtcgacgca gtagagcttc acccggcccg
     6061 cctcgagcag cggggcgatc gcgtcgacca tcccccggtc ctcccagtcg gccgcgctcc
     6121 cctgctcgct cgggaacgcg aggagcgggc gcccccagtg gccgtacgcg aggatccgcc
     6181 cggcggcccc gatcgccggc gagtggagct cgagctcgcg gcggcctact cccacagccg
     6241 gcggagcagg ccgggcaggt ggggagcgag cgcgtcgcgc cagccgatcc agttgtgcgc
     6301 gtcccgcacg acctcgagcc gcacgtcgta gccctgcgac gcgagcgccg acgcgacggc
     6361 ccggttgttc gcgaggttct cctcgaccgt gccgcacgtg aggacggccg ggacgggatc
     6421 gggccactcc gccgcgccga gcacgctccc gacgaagcgg gagatccggt cgaagtgcgg
     6481 gaagctccgc tcgtagcggt cgaagcgctg ccggaagaag ctccccgact ggaggaagag
     6541 cccgccgaag agcccgcgac ggaggcggtg cgcatggacg agcgcgagcg cgccgaggct
     6601 cgccccgagc gcgacccgcg cctcgggacc gccgggcgtc ggcgcggccc gctcgatcgc
     6661 gggcaggagc tcggagacga gcgcgcgccc gtagcgggcg gacgccgagt agtcgcggtt
     6721 gcgctcgcgc gggggtacga gcgcgacgcg aaacggcgga acctccccgg ccgcgacggc
     6781 ggccgcggcg aagcggagga gcgcggagta gtcggcgtac tccggcccgt cgtgcgcgag
     6841 gaggagcggc agcggccgcc cgccgcgcac gcccgccggc gtccagaggc cgacgtcgaa
     6901 cgaggcgtgg agcgtccggc tctcgatcgt caggatctcc cgccggccgg ccggggcatc
     6961 ggcgtcggcg tcgagccagg cgggcgggcg gtaccccggc agctcgagga cggacttctc
     7021 gccgaacgga ccgggcgcgc ggagcgggtt gcccgggtcg cagacggtct cgacgccgcc
     7081 ctccgcgtcc gtcacctcga gccggtactc gatccggtcg acgggcgggc gcgggatcga
     7141 gagcgtccac gggcggccgc ggcccgcgcg ggcgaagtcc ggcccgacgc gcgggcgcca
     7201 gagctcgtgc acgagccgga cacggcgcag ccgcccctcg gggtcggcga gcgagaaggc
     7261 gacctcgtcc gcgacgggcg cggcgccggc gtgcgggcgg gcgaccattg gcgcggagcg
     7321 taccgcgcgc ccgcgccgga gcccgctacg gcacgaacgt gagccggtag agcgcccccg
     7381 actgggcggc gaggtagagc tcgcggtccg gcccctcgcc gaacgccgtc agcgtcggga
     7441 gcga
//
LOCUS       DASTFC010000022         6380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1401127,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000022 DASTFC010000000
VERSION     DASTFC010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401127"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFO88_00650"
     CDS             <1..435
                     /locus_tag="VFO88_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HET9544061.1"
                     /translation="AEPLRAVAAGLAAHEEPVVFPAHPRTTAALAAGAIVLPAHVRVL
                     PPLGYLDFAALASQARVVATDSGGIQKEAYWYGVPCVTLRETTEWVETIEAGWNRLVG
                     TDAAAIAAALADPPRAAERPELYGDGHAAERIAEHLGTMGRR"
     gene            432..1424
                     /locus_tag="VFO88_00655"
     CDS             432..1424
                     /locus_tag="VFO88_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9544062.1"
                     /translation="MTGDTIRIGLAGLGYWGPNLARNFDSLPGAALVAICDADEERLA
                     GAAARFPDARTTTDFDDLLADSIDAVVIATPVPTHAELASRALTAGKHVFVEKPVALS
                     AGEAQDVSSLAAERGLTLMPGHLLLYHPGVAKLREIVLSGDLGRLLVIYGNRQNLGKI
                     RRDENVLWSLGAHDLSVILDLVGEEPEEASARGESFLTKGVEDIVFCYLRFPSGVVAH
                     LHLSWLDPHKMRKITVVGDKKMAVFDDMELDHKVTIYDKGPQEPVHSYGEWMTRSGDI
                     WSPKIPNDEPLRLECARFLDFVRGEADSAPARDNGLAVVRVLEELQRSLERERV"
     gene            1421..2062
                     /locus_tag="VFO88_00660"
     CDS             1421..2062
                     /locus_tag="VFO88_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET9544063.1"
                     /translation="MTARVAETAVVYPGVELGDDVAIGDHAVVGKQPTLGRRSTAARE
                     ELPPLVVGDGCAILAGAVVFAGTRLGRGVVVGDQACVRERCELGDEVVVGRGSLVEND
                     TTVGARTRIQANAYVTAYSTLEEDVFVAPCVVTTNDNFMGRTEARLELMRGPTIRRGA
                     RIGGGAVLLPGIEVGEEAFVGAGAVVVRDVAPRTVVVGNPARVLRAVPDEELL"
     gene            complement(2059..3705)
                     /locus_tag="VFO88_00665"
     CDS             complement(2059..3705)
                     /locus_tag="VFO88_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9544064.1"
                     /translation="MARRLLVAFLAAGALAAPAAAAPGTIAVGVDEDAPRGELGAALA
                     EATGGRLVEDLGPLDALLLAVPDVRRALAAARAHDGVAYAERMAATRRLAFEPNDPLY
                     PDQWYLPTVRAFDHWVAKPPHPPVRVAVIDSGVDGSHPDFAGRIVASRSFVGGSALTD
                     PVGHGTTVAGLIAAGVDEGVGIAGAGISVELLVGKITGANGLIYIQDEARAIRWAVNN
                     GARVINLSLGGPRNPDNPRRDYYSPLEHAAIDYATRNGVLVVAAAGNCSRVRCPEQYA
                     SWPAALPHVLGVGALRPDGQTPAFSNRDPVHVDVAAPGTQMLSTYPAQLMQAGCTPAG
                     YTVCAARPADRSPSGTSFSAPLVAGAAASLLGERLLAGGRLHASQARTILERSARDIP
                     PAGRDRRSGFGLLDVTSAIERLGRPLPPRDRYEPNDDARDKAWRLGGRAVRIRATLDP
                     FDDRRDVYRVPLRRGERLDVRLNGPVRGNSNLYLWRPGTRTLDSGARARRANRLARSA
                     GPSWRERIVYRARTNGYHFVEARLPRGKAGEYTLTVLRSG"
     gene            complement(3706..5295)
                     /locus_tag="VFO88_00670"
     CDS             complement(3706..5295)
                     /locus_tag="VFO88_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET9544065.1"
                     /translation="MRRFLACIAFTVFACVPAAQAAPPVFFVDGHGWGHGIGMPQYGA
                     QGFASRDGRKHDWILAHYYRGTTLGKTSVGKVRILLADRAAGVSIGSASAFKVTDARG
                     RTFTIPKGSHQLGSGLRIRSQNGRLRTLASPARFKRGTSHLRLNGRAYRGEMMLHSSG
                     GRVSAVNHLSLERYLYGVVPDEMPPSWHPEALKAQAVAARSYAVVSRKTSGIFDLYAD
                     TRSQVYNGVPAEDARATSAIDATRGQVVRHNGAVAWTFFHSTSGGRTANIEHVWNTTA
                     RPYLVSVPDPHDDLSPYHKWGPFRYSAKSFRAKLGSSAPRGALVDATANRNASRRISS
                     VTVKGALGTRSLAGTSFQYAFGLRSSWFTIGVLSLDGRSSVTYGKKITLGGVARNVGG
                     GARLERRAWGSNSWTRVRDVATGAVSVQVGPKATAWFRLASPKGKGAGHRVNVATAVV
                     AQIGSARRAIRGGIGPARGGVEVQVQRRVSGSWRTVATATTAGDGSYVARLNVTAGSY
                     RATAKAGPAHARGYSAVLKAG"
     gene            5438..>6380
                     /locus_tag="VFO88_00675"
     CDS             5438..>6380
                     /locus_tag="VFO88_00675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9544066.1"
                     /translation="MADGTDGFPSRYEGPRHVATGGMGEIFVARDTTLGRTVAIKRLS
                     ERFARDESVRRRFTREGLAAARLSGHPNIVTIFDVGESSGRPFLVMEHLTGGTVAERA
                     RAGDVPHDQALAWLRQSADALEEAHRNGIVHRDVKPANLLLDERGDVRVADFGVARIL
                     EEDATHMTMAGTVLGTAGYLSPEQARGEPATPASDVYSLGVVAYELLTGGRPFEGGSA
                     TEEAARHIHQPVPPASERGVGLPRSVDRVFERVLAKDPGTRYATPGAFVSDLAAALEE
                     TERTQVLPPPVAAAPTAATRRMTREREPAATPARRGGP"
BASE COUNT          805 a         2344 c         2429 g          802 t
ORIGIN      
        1 gcggagccgc tccgcgcggt cgccgccggg ctcgcggcgc acgaggagcc ggtcgtcttc
       61 cccgcccacc cgcgcacgac tgccgcgctc gccgccggcg cgatcgtcct gcccgcgcac
      121 gtccgcgtgc ttccgccgct cggctacctc gacttcgccg cgctcgcgtc gcaggcgcgc
      181 gtcgtcgcga cggactcggg cgggatccag aaggaggcgt actggtacgg cgtcccgtgc
      241 gtgaccctgc gggagacgac cgaatgggtc gagacgatcg aggccggctg gaaccggctc
      301 gtgggcaccg acgcggccgc gatcgccgcc gcgctcgccg atccgccgcg cgccgccgag
      361 cgccccgagc tctacggcga cggccacgcg gcggagagga tcgcggagca cctcggtacg
      421 atgggccgcc gatgacgggc gacacgatca ggatcggcct cgcgggcctc gggtactggg
      481 ggccgaacct cgcgcggaac ttcgactcgc tccccggagc ggcgctcgtt gcgatctgcg
      541 acgccgacga ggagcggctc gcgggggcgg ccgcccgctt cccggatgcg cgcacgacga
      601 cggacttcga cgacctgctc gccgactcga tcgacgcggt cgtgatcgcg acgccggtcc
      661 cgacccacgc cgagctcgcc tcccgcgcgc tcacggcggg caagcacgtc ttcgtcgaga
      721 agccggtcgc gctctccgcg ggcgaggcgc aggacgtctc ctccctcgcc gccgagcgcg
      781 gcctcacgct catgccgggc cacctactcc tctaccaccc cggcgtggcg aagctgcgcg
      841 agatcgtcct ctcgggcgac ctcggccggc tgctcgtcat ctacgggaac cgccagaacc
      901 tcgggaagat ccgccgcgac gagaacgtcc tctggagcct cggcgcgcac gacctctcgg
      961 tcatcctcga cctcgtcggc gaggagccgg aggaggcctc ggcccgcggc gagtcgttcc
     1021 tcacgaaggg cgtcgaggac atcgtcttct gctacctccg cttcccctcg ggcgtcgtcg
     1081 cgcacctgca cctctcgtgg ctcgacccgc acaagatgcg gaagatcacg gtcgtcggcg
     1141 acaagaagat ggccgtcttc gacgacatgg agctcgacca caaggtgacg atctacgaca
     1201 agggcccgca ggagcccgtg cactcctacg gcgagtggat gacgcggagc ggcgacatct
     1261 ggagcccgaa gatcccgaac gacgagcccc tccggctcga gtgcgcgcgg ttcctcgact
     1321 tcgtccgcgg cgaggccgac tccgcgcccg cgcgcgacaa cgggctcgcg gtcgtgcgcg
     1381 tgctcgagga gctccagcgc tcgctcgagc gggaacgcgt gtgaccgccc gggtcgccga
     1441 gacggcggtc gtctacccgg gggtcgagct cggcgacgac gtcgcgatcg gcgaccacgc
     1501 ggtcgtcggc aagcagccga cgctcgggcg acgctcgacc gccgcgcggg aagagcttcc
     1561 gccgctcgtc gtcggggacg gctgcgcgat cctcgccggc gcggtcgtct tcgccgggac
     1621 gcggctcggc cggggcgtcg tcgtcggcga ccaggcgtgc gtgcgggagc gctgcgagct
     1681 cggggacgag gtcgtcgtcg ggcgcggctc gctcgtcgag aacgacacga cggtcggggc
     1741 gcgcacgcgg atccaggcga acgcgtacgt gaccgcgtac tcgacgctcg aggaggacgt
     1801 gttcgtcgcg ccctgcgtcg tgacgacgaa cgacaacttc atggggcgca cggaggcgcg
     1861 gctcgagctc atgcgcggcc cgacgatccg ccggggcgcg cggatcggcg gcggggcggt
     1921 gctcctcccg gggatcgagg tcggcgagga ggcgttcgtc ggcgcgggcg ccgtcgtcgt
     1981 gcgcgacgtg gcgccccgga cggtcgtcgt cgggaacccg gcgcgcgtgc tgcgcgcggt
     2041 gcccgacgaa gagctgctct agcccgagcg gaggacggtg agggtgtact cgccggcctt
     2101 gccgcgcggg agccgcgcct cgacgaagtg atagccgttc gtgcgggcgc ggtagacgat
     2161 ccgctccctc cagctcgggc cggcggagcg cgcgaggcgg ttcgcgcggc gcgcgcgggc
     2221 gccggagtcg agcgttcgcg tcccgggccg ccagaggtag aggttgctgt tcccgcgcac
     2281 cggcccgttc agccggacgt ccagccgctc gccgcggcgg agcggaaccc ggtacacgtc
     2341 gcgccggtcg tcgaagggat cgagcgtcgc acggatccgg accgccctcc cgccgaggcg
     2401 ccatgccttg tcgcgcgcgt cgtcgttcgg ctcgtagcga tcgcgcggcg gaagcggccg
     2461 gccgagccgc tcgatcgcgc tcgtcacgtc gaggagcccg aacccggagc ggcggtcgcg
     2521 cccggccggg gggatgtccc tcgcgcttcg ctcgaggatc gtccgcgcct ggctcgcgtg
     2581 cagcctcccg ccggcgagca gccgctcgcc gaggagcgag gccgcggcgc cggcgacgag
     2641 cggggccgag aacgacgtcc cgctcgggct cctgtcggcg ggccgcgccg cgcagaccgt
     2701 gtagccggcc ggggtgcagc ccgcctgcat gagctgcgcc gggtacgtcg agagcatctg
     2761 cgttcccggc gccgcgacgt cgacgtgcac cgggtcgcgg ttcgagaagg ccggcgtctg
     2821 cccgtccggc cggagcgcgc cgaccccgag cacgtgcggg agcgcggccg gccagctcgc
     2881 gtactgctcg gggcagcgca cgcgcgagca gttcccggcg gcagcgacga cgaggacgcc
     2941 gttccgcgtc gcgtagtcga tcgcggcgtg ctcgagcggc gagtagtagt cccgccgcgg
     3001 gttgtccggg ttgcgcggcc cgccgaggct caggttgatc accctcgcgc cgttgttcac
     3061 cgcccagcgg atcgcgcgcg cctcgtcctg gatgtagatc aggccgttgg cgccggtgat
     3121 cttccccacg aggagctcga cggagatccc ggcgcccgcg atcccgacgc cctcgtcgac
     3181 gccggcggcg atgagcccgg cgaccgtcgt cccgtggccg accgggtcgg tgagggcgga
     3241 gccgcccacg aagcttctcg aggcgacgat gcggccggcg aagtccgggt gggagccgtc
     3301 cacgccggag tcgatcacgg cgacgcggac gggcgggtgc ggcggcttcg cgacccagtg
     3361 gtcgaacgcg cgcaccgtgg ggaggtacca ctggtcgggg tagagcgggt cgttcggctc
     3421 gaacgcgagc cggcgcgtcg ccgccatccg ctccgcgtac gcgactccgt cgtgcgcacg
     3481 ggcggccgcg agcgcgcggc gcacgtccgg cacggcgagg aggagcgcgt cgagcgggcc
     3541 gaggtcctcg acgagccgcc cgccggtcgc ctcggcgagc gcggcgccga gctcgccgcg
     3601 cggcgcgtcc tcgtcgacgc cgacggcgat cgtcccgggc gcggcggccg cgggcgcggc
     3661 gagcgccccc gcggcgagga aggcgacgag gaggcggcgg gccacctagc cggccttgag
     3721 gacggccgag tacccgcgcg cgtgcgcagg cccggccttc gcggtcgcgc ggtagctgcc
     3781 ggccgtgacg ttcagccgcg cgacgtagga cccgtccccc gccgtggtcg ccgtcgcgac
     3841 cgtgcgccac gagccgctca cgcggcgctg cacctgcacc tcgaccccgc cgcgggccgg
     3901 cccgatcccg ccgcggatgg cccgccgcgc gctcccgatc tgggcgacga cggccgtggc
     3961 gacgttcacg cggtgccccg cgcccttgcc cttcggggag gcgagccgga accacgcggt
     4021 cgccttcggc ccgacctgca cgctcacggc gccggtcgcg acgtcgcgca cgcgcgtcca
     4081 cgagttgctc ccccaggcgc gccgctcgag ccgggcgccg ccgccgacgt tgcgggcgac
     4141 gccgccaagc gtgatcttct tcccgtacgt gacggacgag cggccgtcga gcgagaggac
     4201 gccgatcgtg aaccagctcg agcggaggcc gaacgcgtac tggaacgacg tccccgcgag
     4261 gctccgcgtc ccgagcgcgc ccttgacggt cacgctcgag atgcgccgcg aggcgttccg
     4321 gttcgcggtc gcgtcgacga gcgcgcccct cggcgccgac gagccgagct tcgcgcggaa
     4381 gctcttcgcc gagtaccgga acgggcccca cttgtggtac ggcgagaggt cgtcgtgcgg
     4441 gtcggggacg gagacgaggt acggccgcgc ggtcgtgttc cagacgtgct cgatgttcgc
     4501 ggtccggccg ccggacgtcg agtggaagaa cgtccaggcg acggcgccgt tgtgccggac
     4561 gacctggccg cgcgtggcgt cgatcgcaga cgtcgcgcgg gcgtcctcgg cggggacgcc
     4621 gttgtagacc tggctccgcg tgtccgcgta gaggtcgaag atcccgctcg tcttgcgcga
     4681 cacgaccgcg tacgagcgcg cggcgaccgc ctgggccttg agcgcctccg ggtgccagct
     4741 cggcggcatc tcgtccggga cgacgccgta gaggtagcgc tcgagcgaaa ggtggttcac
     4801 ggccgagacg cgtccgccgg aggagtgcag catcatctcc ccgcggtacg cgcgcccgtt
     4861 caggcgcagg tgcgacgtgc cgcgcttgaa gcgcgccggg ctcgcgagcg tgcggagccg
     4921 gccgttctgc gagcggatcc ggagcccgga cccgagctgg tggctcccct tcgggatcgt
     4981 gaacgtccgc ccgcgcgcgt ccgtgacctt gaacgcgctc gccgacccga tcgagacgcc
     5041 ggccgcccgg tcggcgagta ggatccgaac cttgccgacg gaggtcttcc cgagggtcgt
     5101 gccccggtag tagtgggcga ggatccagtc gtgcttgcgc ccgtcgcgcg acgcgaagcc
     5161 ctgggcgccg tactgcggca tcccgatccc gtgcccccag ccgtggccgt cgacgaagaa
     5221 cacgggcggg gcggcctgcg cggcgggcac gcacgcgaag acggtgaagg cgatgcaggc
     5281 gaggaaacga cgcatgacga tccggagggc gaccccaccc cagggtagag aagcgcggta
     5341 atgattgtcc aaaggagccg ttcgcggcag gccgcccggc gccttcccgc gacggttccc
     5401 gcgtttcgcc ccgccggggc atggcaacct gcaggtcatg gccgacggga ccgacggctt
     5461 cccgtcccgc tacgagggcc cgcgccacgt cgcgacgggc gggatgggcg agatcttcgt
     5521 cgcgcgcgac acgacgctcg ggcggacggt ggcgatcaag cgactctcgg agcgcttcgc
     5581 gcgcgacgag tccgtgcgcc ggcgcttcac gcgcgagggc ctcgcggcgg cgcgcctctc
     5641 cgggcatccg aacatcgtca cgatcttcga cgtcggcgag tcgagcgggc ggccgttcct
     5701 cgtcatggag cacctgaccg gcgggacggt ggccgagcgc gcgcgggccg gggacgtccc
     5761 gcacgaccag gcgctcgctt ggctccggca gtccgcggac gcgctcgagg aggcgcaccg
     5821 caacgggatc gtccaccggg acgtgaagcc ggcgaacctc ctcctcgacg agcgcggcga
     5881 cgtacgggtc gccgacttcg gggtcgcgcg gatcctcgag gaggacgcga ctcacatgac
     5941 gatggccggc accgtgctcg gcacggccgg ctacctctcg cccgagcagg cgcgtggcga
     6001 gccggcgacg cccgcgtcgg acgtctactc gctcggcgtc gtcgcgtacg agctcctgac
     6061 cggagggcgg ccgttcgagg gcggctccgc gacggaggag gcggcgcgcc acatccacca
     6121 gccggtgccg ccggcgtccg agcgcggcgt ggggctgccc cgctcggtcg accgcgtgtt
     6181 cgagcgcgtg ctcgcgaagg atcccggcac gcggtacgcg acgccggggg cgttcgtctc
     6241 cgacctcgcc gccgcgctcg aggagacgga gcggacgcag gtactccccc cgcccgtcgc
     6301 ggccgcgccg accgcggcga cccggcggat gacccgcgag cgggagcctg cggcgacgcc
     6361 tgcgcggcgc ggcgggccgg
//
LOCUS       DASTFC010000023        15576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_939695,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000023 DASTFC010000000
VERSION     DASTFC010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 15576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15576
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939695"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFO88_00680"
     CDS             <1..1071
                     /locus_tag="VFO88_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9544067.1"
                     /translation="KGTHFAAPTEDGIVVAEELARRFRLPKWRYTNSGSESTMDAIRI
                     ARAFTGRDTIVKIFGSYHGHHDYAMVSIGVPYDEIGDRENYASLPYGEGIPQSVVDLT
                     IPVPFNDADAMERRIERLIEEDRMPACVIMEAAMMNLGIVLPEPGYLEAVRELTERHG
                     IVLIFDEVKTGLCIAAGGAVERFGVVPDMVTLAKGLGAGLPSGAIGATEEIMELVENE
                     RVYQVGTFNGNPASMAAARASLEEVLTPEAYEHLDAMNERLMTGCEAVIEKYALPGYA
                     VGVGAKGCVTFSPSKITDYESFKATQDGELMDLAWLYNMNRGIFMTPGREEEWTLSVT
                     HSLEDADAFVAVVEEMAADLTA"
     gene            1097..1519
                     /locus_tag="VFO88_00685"
     CDS             1097..1519
                     /locus_tag="VFO88_00685"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9544068.1"
                     /translation="MIHFDTSVHIDRPREKVFGTIADPETYPRWNSAVTDVTRLAEAG
                     RYRMLRTLPTGPAENLLEIVSASAPEEVVLRASDGPTPFTYRYRLEEHGGGTDVHLDA
                     DVDLGGVAGLLGPVAGRFVRRGVDENLATLKQELERGR"
     gene            complement(1552..5109)
                     /locus_tag="VFO88_00690"
     CDS             complement(1552..5109)
                     /locus_tag="VFO88_00690"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9544069.1"
                     /translation="MSRMVVTPLFASFTFATGAAALAAWGDALAEPSVRSGAIAAHWL
                     LKLGVVLAFCIFVFLREPSRRPSRDPVAFVACTAAILGVIAVREPSETASTALVVAGE
                     LVTVAFAVWLLVSVLALGRCFGVLPEVRGLVTRGPYRHVRHPVYLGELGALTGLVLAA
                     PSLWNAGALAVSFAAQGVRMRLEERALAAEFPEYAEYAARTPRLVPRLRLPVRARPAG
                     GLPAATITLAVAVSLAVIPAATAAPAVPELAGPPAETVVETLPAFSWDAVPGADRYEW
                     QIAADPAFNSTVLGANLDRFFTKNLHATMTKVVPNGTYWWRVRAVAPDGAVSAWSPGR
                     SITKVWAVAPELAAPADGATITFPADRFALSWDPVPGAARYLVRIGTDPTLASLVLSG
                     TGTETQATTYTLLGPLAPGKTYYWGITPIDAAGNRGEPSPVWSFTWVWPSATATRVVD
                     VASAPELDDHRFEWDPVSGAAGYQVEVSSSSDFAPGSSACCPVSSITKLTTIGTSYSP
                     AVVLPNNNRYYWRVRAIDASGNAGMWNVGPEFSKGFDNQDPSIANLRMLDGSPGEADV
                     ATSTPVVTWDPVPGASAYEVEVTRFVGGCQWTATQEHWRSTTATTAWTPLGWGWNNQK
                     PYQTSRNVATDTPFLVAGHSYCVRVTALDRPANSVNRIRSAETYLPDESTPAFTWLGP
                     PSSAPCSPSCSNGAPGADDYLAPARGSTSPTAPLFRWKPLEGAQSYFVIVAKDADFTN
                     VVDYAFTRIPAYAPRWTTGPRTYPDETTLYYWAVLPASGTDGSGVVTAPRFSAPASFH
                     KQSAPPELLAPAAGTVVSGAPTFHWAPVRGAYRYRLEVASDESFANLVDSIVTDATSY
                     SSSATYPADTTLYWRVRADAEDVHEGAGIGLRWSLPRAFSKTLPAPVPSADNPTAGDF
                     VPTWTWDPVPGAVSYEFQLRFPNGSVRNFNGVPSASATPTVLKGTGIWSWQVRANFPR
                     VSTTGVTPGPWSGSRSFARTIREPAGPAEEVSGRRVVVGWTPKLHALNYRVQISTRAD
                     FGTFVQTTTTENPTYAPLLTQYPYVAGGTYYWRVAASDDTTANIGDFTQPRSFTLPPQ
                     AILKAGSRITVKVRRTTRALRVTGVVTPAHRGAVTVTLARRSRGAFRAVATRAAQLSA
                     KSTYATSFPRPARGRCRVTATFPGDRDHLKSTVRLKVRC"
     gene            complement(5106..6023)
                     /locus_tag="VFO88_00695"
     CDS             complement(5106..6023)
                     /locus_tag="VFO88_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9544070.1"
                     /translation="MLIFVVGLFLTGIAVALLLRAAFLPRLNMAETIETIDGYGYAAH
                     QPEQSSGGGVRAGLDDLAGLVGSLVGKRLGSIREEQLRRELMAAGIYTMPPGRFLGYR
                     IISLLAVPATWIWVVVTIGVPGLIGVLGLVVAVFAGWSTPLVIVRNRARRRLEQIDYE
                     TPELIDVLVVTVEAGLGLASSLQVASERLRGPLGEEMRLALQEQSLGLQTTEALRNML
                     DRADTPALRAFVRSIVQGESLGVSTGDIMRNLADEMRKRRRAAAEERAQKAPVKILFP
                     LAFLIFPAMFIVLLGPAVFQFIEGFGAAR"
     gene            complement(6036..7925)
                     /locus_tag="VFO88_00700"
     CDS             complement(6036..7925)
                     /locus_tag="VFO88_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9544071.1"
                     /translation="MRSARRLIALAGVLLAAAALPGAAAGATDLRLRELEGARFPDRA
                     FLLSLPDAAALDPGLVTVRENGEPVTDLTLLPASEAGAGEFAVVLVIDASNSMRGEAI
                     EGALEAARAFAAERGPNQAIAVLAFNGQTRVVLPFTTSAEEIDEALAAAPPLAYGTLM
                     RDAVDEAVGLLAAERATVGSVVVLSDGADTGSSVSTAALAERARRAGARVFTVGLRSH
                     RFDGGPLEALAGATGGRYAEASSPEALQRVFAELGSRLASEYLIRYRSEARPDVRVHV
                     AVRVEEIDGVGVTEYVTPAVAADPGPPFRRPLLERFVQSQAGVVATSFVFALVVALGL
                     VALIRPRPRTLRRRMAEFVSVTLRERGSKRGAQGDLVLARAEKSLAETRWWARFGEEL
                     EVGEIRIPAMQILLATLVGTLAAAWLVLTLTGALVFAPLALGVPLIVRGIIARRAEKQ
                     RNLFAEQLPDNLQVISSALRAGHSLSSALAVLVEDCSEPSRREFRRVVADERLGAPLD
                     ETLVEVARRMQSRDLEQVALVAALQRETGGNTAEVLDRVAETVRGRFELRRLVRTLTT
                     QGRMSRWVLSLLPVGLLFVMSALNPGYVAPLFTNPVGRVLLVLGAVMIVTGSLVIKRI
                     VNIKV"
     gene            complement(7922..9277)
                     /locus_tag="VFO88_00705"
     CDS             complement(7922..9277)
                     /locus_tag="VFO88_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET9544072.1"
                     /translation="MDLHERLSTTTRPVAPTNGRDPFSELKNRLHLAVIGELGPQLFN
                     VSMDPNAIRDRVTADIRTHLAKEQGISRDDRDRLEAEIADDILGHGPLERLLADESVT
                     EIMVNGPFDVWVERQGRLYETTVRFIDESHLRRIINKMVAQVGRRIDESSPMVDARLP
                     DGSRVNAVIPPLSLSGPLVTVRKFSKQRLDLTDLVRLGTLSTETVEFLQRCILAGLNI
                     LISGGTGSGKTTLLNALSTAIPDADRIVTIEDAAELRLNQRHVLRLEARPKNIEGEGE
                     IPIRELVRNSLRMRPDRIIVGEVRGAEALDMLQAMNTGHDGSLCTVHANAPRDALARV
                     ETMVLMAGYELPVRAIRQQVASALDMIIHLERLEDGSRRVTAITEVGRMESEVITLQD
                     IFEFKVEQVTSDRVVIGSLRSTGLRPTFLHKFEKRGVALPAGLFSDPRAHVADFQRQA
                     R"
     gene            complement(9277..10458)
                     /locus_tag="VFO88_00710"
     CDS             complement(9277..10458)
                     /locus_tag="VFO88_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9544073.1"
                     /translation="MSAPIRILVAVDDGVESELVNASLPSDNGVQIVGVIDGLQESWQ
                     TLQEVPTDLLAVVCGDYSDRAITLIDGAVKQRPERPVVVLSDGSPNGFVKRVFEVGAD
                     DIVSLPANPEQIAFTFEKAMVRKQGTAGAGGVALAPMICVLGPKGGTGKTLTACNLGV
                     ALAGAGNRVAIVDLDLQFGDVALSLGLAPERTIYDLAKSGGSLDADKVAGYLAEHPSG
                     VRVLVAPTRPDHAGAITTEFLREVYAALRASHDFVVVDTPPGFTPEVIASIDSSSHVC
                     VVGMLDSLSLKNTKLGLETLELMGYSRERTRFVLNRADSRVGITRDDVVAIVGRTPDV
                     LVPSDRDIPRSVNEGTPIVQAGRRSDAAKAFGQLAALYETRDANGGKRRRFGRRKKAS
                     V"
     gene            complement(10455..11240)
                     /gene="cpaB"
                     /locus_tag="VFO88_00715"
     CDS             complement(10455..11240)
                     /gene="cpaB"
                     /locus_tag="VFO88_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9544074.1"
                     /translation="MLSTRGGTIAISAVAGLVAAAIILVYLNRYRESVGTATQPMTVL
                     VAKDLIEKGTPGNMVGSDGLFQAVTTPRSEVKEGAITDPAALRGTVAVDDVYPGEQLT
                     ASAFSGAGADTVAARISADERGIAVPIDNAHGMIGNVSAGDHVDVYAGFNVRKVLPNG
                     SPDPNAMDRPVLKLIVEDVVVLDAPKGAGAAVGGAQNQAVTLRLSEADAARVAFSSDN
                     GVVWIVLRPPAGAEPTTPDIVTLETVLFGVPPVAAAKSLGAKP"
     gene            complement(11272..13203)
                     /locus_tag="VFO88_00720"
     CDS             complement(11272..13203)
                     /locus_tag="VFO88_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET9544075.1"
                     /translation="MSRVLELALRGRRREDGGVLVMVVLFIPAVVLFVVFVVDVGNWF
                     VHKRHLQLQADAGALAGGGSFTWPCSNDTIEERARQYAGDPGNSTPYNTQIPPTDGAN
                     VHVLINSEDFWNEGGTDYSDGGPPCSARMVDVKITESNLPWYFRIATVPAINARARVE
                     IQGLFQTAGALPVAVPDVEPNVARAFFVNEATGAVLASTPLAKTATTGGTTVWDNSAA
                     PLSVPVSAERIGVRIALGGGSSTDCGGRFVACYDLGGPNGLLYVRGWSSAGSGAQPNP
                     PIARDVRLFPGSCPDAYFTTEASSCSVGVSAVVDFGTNDPVGANGASVTAVVGGVTRP
                     LTYDSASGRWVSTGTDFFSVAPAAGPLPVELRWEQTIGQVNVGGRLEDCKPNGNKCVG
                     TLGVVQRTFAGSEARSGAIKVAEVSEGGVAGANSFELGTSPNLVVRIAVAGNLVHATS
                     VNDPPILLRVSGSQNQTVDCDPALPNIREEIRYGCAPQYQINSGSTCPATAPTLWASA
                     QPWQCTAVQTGGAIGQVEQGLRDRILGGAAQCTSPNNWASFPDLPPGDPRIVPVFVTP
                     FGTFEGSGNDVVPVKNFAAFYVTGWFSSPCSGDDPVPDRGYIVGRFIKYVFSLNSGGG
                     SGQLCDFDAFGSCIAVMTE"
     gene            complement(13200..13670)
                     /locus_tag="VFO88_00725"
     CDS             complement(13200..13670)
                     /locus_tag="VFO88_00725"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HET9544076.1"
                     /translation="MLPSRFAGRNPEEGAALVEFALVLPVLLVLLLGMLDFGKAFNYW
                     IDQTHLANEGARWAAVNKNPGGGSLQQYVRSQANTPELRDGGTASVAGPIQICITFPN
                     GTSQVGDPVRVTASTTYNWLPFLGSRLGIAQTTMTASATMRLEAAPSVYGAGCS"
     gene            complement(13684..13851)
                     /locus_tag="VFO88_00730"
     CDS             complement(13684..13851)
                     /locus_tag="VFO88_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544077.1"
                     /translation="MLGNIVNRSNASHEDGQALVEYALIIAFVAIACTATLGLLGTTV
                     DAAIQGAVSAF"
     gene            complement(13936..14097)
                     /locus_tag="VFO88_00735"
     CDS             complement(13936..14097)
                     /locus_tag="VFO88_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET9544078.1"
                     /translation="MIEFFNALRNREEGQALVEYGLILALVSVACVATLGALGTGIDG
                     ILQTIVGNL"
     gene            14329..>15576
                     /locus_tag="VFO88_00740"
     CDS             14329..>15576
                     /locus_tag="VFO88_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544079.1"
                     /translation="MDARTGKRRWADAGGGRRALASALLAVGSALLALVRFASWAAPD
                     RARPGGTGPATAEPGPSPEAREPEAADAERDDTRRTAASPSRSRRLASSLVFAALFFS
                     GAALSAVAGDTIGSPEPDGPRAVTCTGPAADAGEDAPEGESPGDPCETGETATETTPE
                     PEAPPAAGDTGETPPASDADAGAEPPAEIPAESEGGGDVPPPTPAPAPHAHEHSAGSG
                     APPVLGGASPAAPRALDPEASADDSIVWLHRVLPDPTPPARRLAPAFARELRRTAAEH
                     RIGWSLLLAVVRAHGNTGRVPVGPGTLDRIAGRLAAETDALGSRNAWHVALRYSGRTA
                     FADRTLALDRYNRGVGLRALVTGLEAAKDDLARRVLADPAIEIYTAGRADVAAGRTDV
                     RILALLRYLRIAHGSVTVSSLTSG"
BASE COUNT         2207 a         5605 c         5707 g         2057 t
ORIGIN      
        1 aagggcacgc acttcgccgc gccgaccgag gacgggatcg tcgtcgccga ggagctcgcc
       61 cgccgcttcc gcctgccgaa gtggcgctac acgaactccg gctccgagtc gacgatggac
      121 gcgatccgca tcgcgcgcgc cttcaccggc cgcgacacga tcgtgaagat cttcggctcc
      181 taccacggcc accacgacta cgcgatggtc tcgatcggcg tcccgtacga cgagatcggc
      241 gaccgcgaga actacgcctc cctcccgtac ggggagggga tcccgcagtc ggtcgtcgac
      301 ctgacgatcc cagtgccgtt caacgacgcg gacgcgatgg agcgccggat cgagcgcctg
      361 atcgaggagg accgcatgcc cgcctgcgtg atcatggagg cggcgatgat gaacctcggg
      421 atcgtgctcc ccgagcccgg ctacctcgag gcggtgcgcg agctgaccga gcgccacggg
      481 atcgtcctca tcttcgacga ggtgaagacg ggcctctgca tcgcagccgg cggggcggtc
      541 gagcggttcg gcgtcgtccc ggacatggtc acgctcgcca aggggctcgg cgccgggctc
      601 ccgtcgggcg cgatcggcgc gaccgaggag atcatggagc tcgtcgagaa cgagcgcgtc
      661 taccaggtcg ggacgttcaa cggcaacccg gcctcgatgg ccgcggcgcg cgcgagcctc
      721 gaggaggtgc tgacgcccga ggcgtacgag cacctcgacg cgatgaacga gcggctcatg
      781 accggctgcg aggccgtgat cgagaagtac gcgctgcccg gctacgcggt cggcgtcggc
      841 gcgaagggct gcgtcacgtt ctcgccctcg aagatcacgg actacgagtc gttcaaggcg
      901 acgcaggacg gcgagctcat ggacctcgcg tggctctaca acatgaaccg gggcatcttc
      961 atgaccccgg ggcgcgagga ggagtggacg ctctccgtca cgcactccct cgaggacgcc
     1021 gacgcgttcg tcgcggtcgt cgaggagatg gccgccgacc tgacggcata gcttcccacc
     1081 caggagagga ggggacgtga tccacttcga cacctccgtc cacatcgacc ggccgcggga
     1141 gaaggtcttc ggcacgatcg ccgatcccga gacctacccg cggtggaact ccgcggtcac
     1201 cgacgtgacg cgccttgccg aggcggggcg ctaccgcatg ctccgaactc tgccgacggg
     1261 gcccgccgag aacctcctcg agatcgtgag cgcgtccgct cccgaggagg tcgtccttcg
     1321 tgcctccgac ggcccgacgc ccttcacgta ccgctacagg ctcgaggagc acggcggcgg
     1381 gacggatgtc cacctcgacg cggacgtcga tctcgggggc gtagccggtt tactcgggcc
     1441 ggtcgcgggc aggtttgtcc gccgcggcgt cgacgagaac ctcgccacgc tcaagcagga
     1501 gctcgagcgc ggccggtaag aggtcgtccc tgcggcagcg accgcgcccg ctcagcacct
     1561 caccttcagc cgcaccgtgc tcttcaggtg gtcgcggtca cccgggaagg tcgccgtcac
     1621 acggcacctc ccccgcgccg ggcgcgggaa cgaggtcgcg tacgtgctct tcgccgagag
     1681 ctgcgccgcc cgtgtcgcga ccgcgcggaa cgcgccgcgg ctcctccggg cgagcgtgac
     1741 ggtcaccgcg ccgcggtgcg ccggcgtcac gacgccggtc acgcggagcg ccctggtcgt
     1801 ccgccgcacc ttcacggtga tccggctccc cgccttgagg atcgcctgcg gcggaagcgt
     1861 gaacgagcgc ggctgcgtga agtcgccgat gttcgcggtc gtgtcgtcgg acgcggccac
     1921 gcgccagtag tacgtcccgc ccgcgacgta ggggtactgg gtgagcagcg gtgcgtacgt
     1981 cgggttctcg gtcgtcgtcg tctggacgaa cgtcccgaag tcggcgcggg tcgagatctg
     2041 cacgcggtag ttgagcgcgt ggagcttcgg cgtccagccg acgacgacgc gacggccgga
     2101 gacctcttcc gccggccccg ccggctcgcg gatcgtgcgc gcgaacgagc gcgagccgct
     2161 ccacgggccg ggcgtgacgc ccgtcgtcga gaccctgggg aagttcgccc gcacctgcca
     2221 ggaccagatg cccgtgccct tgaggacggt cggcgtcgcc gacgcggacg ggacgccgtt
     2281 gaagttccgc acgctcccgt tcgggaagcg cagctggaac tcgtacgaga ccgcgcccgg
     2341 caccggatcc cacgtccacg tcgggacgaa gtcgccggcc gtcgggttgt cggcgctcgg
     2401 aaccggcgcc ggaagcgtct tcgagaacgc gcgcggcaac gaccaccgga ggccgatgcc
     2461 cgcgccctcg tggacgtcct ccgcgtctgc gcggacgcgc cagtagagcg tcgtgtcggc
     2521 cggatacgtc gcgctgctcg aatacgaggt cgcgtcggtc acgatcgagt cgacgaggtt
     2581 cgcgaagctc tcgtcgctcg ccacctcgag ccggtagcgg tacgcgcccc gcaccggcgc
     2641 ccagtggaac gtcggcgcgc cgctcacgac cgtgcccgcc gcgggcgcga gaagctcggg
     2701 cggcgccgac tgcttgtgga acgacgcggg cgcggagaag cgcggagccg tgacgacgcc
     2761 gctcccgtcg gtcccgctcg ccgggaggac ggcccagtag tagagcgtcg tctcgtccgg
     2821 atacgtccgc ggcccggtcg tccagcgcgg ggcgtaggcg gggatccgcg tgaacgcgta
     2881 gtcgacgacg ttcgtgaagt ccgcgtcctt cgccacgatg acgaagtagc tctgcgcgcc
     2941 ctcgagcggc ttccaccgga agagcggggc cgtcggcgag gtcgagccgc gcgccggggc
     3001 gaggtagtcg tccgcgcccg gcgcgccgtt cgagcacgac ggcgagcacg gtgcgctaga
     3061 cggcggcccg agccacgtga aggccggggt gctctcgtcg ggcaggtagg tctccgcgct
     3121 ccggatccgg ttgaccgagt tggccggccg gtcgagagcc gtgacgcgga cgcagtacga
     3181 gtgcccggcg acgaggaacg gcgtgtccgt cgcgacgttc ctcgacgtct ggtacggctt
     3241 ctggttgttc cagccccagc cgagcggcgt ccacgcggtc gtcgccgtgg tgctccgcca
     3301 gtgctcctgc gtggccgtcc actggcagcc gccgacgaag cgcgtcacct ccacctcgta
     3361 cgccgaggcg cccgggacgg gatcccacgt gacgacgggc gtgctcgtcg cgacgtccgc
     3421 ctcgcccggg gagccgtcga gcatcctgag gttcgcgatg ctcgggtcct ggttgtcgaa
     3481 ccccttcgag aactccggcc cgacgttcca catgcccgcg ttcccgctcg cgtcgatcgc
     3541 acggacgcgc cagtagtagc ggttgttgtt cgggagcacg acggcgggcg agtacgacgt
     3601 cccgatcgtc gtgagcttcg tgatcgagct gaccggacag cacgcggacg agcccggcgc
     3661 gaagtcgctc gagctcgaga cctcgacctg gtagccggcc gcgcccgaga cgggatccca
     3721 ctcgaaccgg tggtcgtcga gctcgggggc gctcgccacg tcgacgacgc gggtggcggt
     3781 cgcggacggc cagacccacg tgaacgacca caccggcgac ggctcgccgc ggttccccgc
     3841 cgcgtcgatc ggggtgatgc cccagtagta cgtcttgccc ggcgcgagcg gcccgaggag
     3901 cgtgtacgtc gtcgcctgcg tctcggtccc cgtcccggag aggacgagcg acgcgagcgt
     3961 cgggtccgtg ccgatccgga cgaggtagcg ggcggcgccg ggaaccgggt cccacgagag
     4021 cgcgaagcgg tcggccggga aggtgatcgt cgcgccgtct gccggcgccg cgagctcggg
     4081 cgcgacggcc cagaccttcg tgatcgaacg gccgggcgac caggccgaca cggcgccgtc
     4141 cggcgcgacg gcgcggacgc gccaccagta cgtcccgttc gggacgacct tcgtcatcgt
     4201 cgcgtggagg ttcttcgtga agaagcggtc gaggttcgcg ccgagcacgg tcgagttgaa
     4261 ggccgggtcg gccgcgatct gccattcgta gcggtccgcg cccgggacgg cgtcccagga
     4321 gaacgccggg agggtctcga cgaccgtctc ggcgggcggg ccggcgagct ccgggacggc
     4381 gggcgcggcg gtcgcggccg ggatcacggc gagcgagacg gcgaccgcca gcgtgatcgt
     4441 cgcggcgggc aggccgccgg ccgggcgcgc acgcacgggc agccggagcc gcggcacgag
     4501 gcgcggcgtc cgggcggcgt actcggcgta ctcggggaac tccgcggcga gcgcgcgctc
     4561 ctcgaggcgc atccgcacgc cctgcgccgc gaacgacacg gcgagcgcgc ccgcgttcca
     4621 gagcgacggg gccgcgagga cgaggcccgt cagggcgccg agctcgccga ggtagacggg
     4681 atggcggacg tgccggtacg ggccgcgcgt cacgagcccg cggacctccg ggaggacgcc
     4741 gaagcaccgt ccgagcgcga ggacggagac gaggagccag acggcgaacg cgacggtgac
     4801 gagctccccg gcgacgacga gcgccgtgct cgccgtctcg gacggctcgc gcaccgcgat
     4861 caccccgagg atggcggccg tgcacgcgac gaacgcgacc gggtcgcggg acggccgcct
     4921 cgacggctcc cgcaggaaga cgaagatgca gaacgcgagg acgacgccga gcttcaggag
     4981 ccagtgcgcg gcgatcgcgc cggagcggac gctcggctcc gccagcgcgt cgccccaggc
     5041 ggcgagcgcc gcggcgccgg tcgcgaacgt gaacgacgcg aagagcggcg tgacgaccat
     5101 ccggctcatc gcgcggcccc gaatccctcg atgaactgga acacggccgg cccgagaagg
     5161 acgatgaaca tcgccgggaa gatgaggaag gcgagcggga agaggatctt caccggcgcc
     5221 ttctgggccc gctcctcggc cgcggcacgg cggcgcttgc gcatctcgtc ggcgaggttc
     5281 cgcatgatgt cgccggtcga gacgccgagc gactcgccct ggacgatcga gcggacgaac
     5341 gcacggagcg ccggcgtgtc ggcgcggtcg agcatgttcc ggagcgcctc cgtcgtctgc
     5401 aggccgaggc tctgctcctg gagggccagg cgcatctcct cgcccagggg cccgcgcagg
     5461 cgctcggagg cgacctggag ggagctcgcg agcccgaggc cggcctcgac ggtgacgacg
     5521 aggacgtcga tcagctcggg cgtctcgtag tcgatctgct cgagccgccg ccgcgcccgg
     5581 ttgcggacga tgacgagcgg cgtcgaccag ccggcgaaga ccgcgacgac gagcccgagc
     5641 accccgatga ggccgggaac gccgatcgtg acgaccaccc agatccacgt cgccgggacg
     5701 gcgaggagcg agatgatccg gtagccgagg aacctgccgg ggggcatcgt gtagatcccc
     5761 gccgccatga gctccctgcg gagctgctcc tcgcggatgc tcccgaggcg cttgccgacg
     5821 aggcttccga cgagcccggc gaggtcgtcg agcccggcgc ggacgccgcc tcccgagctc
     5881 tgctccggct ggtgcgcggc gtagccgtag ccgtcgatcg tctcgatcgt ctctgccatg
     5941 ttcagccgcg gcaggaaggc ggcccggagg agcagcgcca cggcgatgcc cgtcaggaac
     6001 agaccgacca cgaagatgag catccgaacc cctcctcaga ccttgatgtt cacgattcgc
     6061 ttgatgacga gcgacccggt cacgatcatc acggcgccca gaacgaggag cacgcgcccg
     6121 accgggttgg tgaagagcgg cgcgacgtag cccgggttga gcgccgacat gacgaacagc
     6181 aacccgaccg gcaggagcga cagcacccag cgcgacatgc ggccctgcgt cgtcagcgtc
     6241 cggacgagcc ggcgcagctc gaagcggccg cggacggtct ccgccacgcg gtcgagcacc
     6301 tcggccgtgt tcccgcccgt ctcgcgctgg agggcggcga cgagcgcgac ctgctcgagg
     6361 tcccggctct gcatgcgacg cgccacctcg acgagggtct cgtcgagcgg ggcgccgagc
     6421 cgctcgtccg cgacgacgcg gcggaactcg cggcgcgagg gctccgagca gtcctcgacg
     6481 agcacggcga gcgcgctcga gagactgtgc ccggcgcgca gcgccgacga gatgacctgg
     6541 aggttgtccg ggagctgctc ggcgaagagg ttccgctgct tctccgcccg gcgggcgatg
     6601 atcccgcgca cgatgagcgg gacgccgagc gcgagcggcg cgaagacgag cgcgccggtg
     6661 agcgtgagga cgagccacgc ggcggcgagc gtcccgacga gcgtggcgag gaggatctgc
     6721 atcgcgggga tgcggatctc gcccacctcg agctcctcgc cgaagcgcgc ccaccagcgc
     6781 gtctccgcga gcgacttctc cgcgcgcgcg aggacgaggt cgccctgggc cccccgcttc
     6841 gagccgcgct cgcgcagcgt gaccgagacg aactcggcca tccgccggcg cagcgtccgg
     6901 gggcgcgggc ggatgagcgc gacgaggccg agcgcgacga cgagcgcgaa gacgaagctc
     6961 gtggcgacga cgccggcctg cgactggacg aaccgctcga ggagcgggcg ccggaacggc
     7021 gggccggggt ccgcggcgac ggcgggcgtc acgtactcgg tcaccccgac gccgtcgatc
     7081 tcctcgacgc gcaccgcgac gtggacgcga acgtccggcc gcgcctccga ccggtagcgg
     7141 atcagatact cgctcgcgag gcgcgagccg agctcggcga agacgcgctg gagcgcctcg
     7201 ggggaggagg cctccgcgta ccggccgccc gtcgcgcccg cgagggcctc gagcgggccg
     7261 ccgtcgaacc ggtgcgaacg cagcccgacg gtgaagacgc gggcgcccgc ccggcgggcg
     7321 cgctcggcga gcgccgccgt cgagaccgag ctccccgtgt ccgcgccgtc cgagagcacg
     7381 acgaccgagc cgaccgtcgc ccgctcggcg gcgagcagcc cgaccgcctc gtcgacggcg
     7441 tcgcgcatga gcgtcccgta cgcgagcggc ggggcggcgg cgagcgcctc gtcgatctcc
     7501 tcggcgctcg tcgtgaaggg gaggacgacg cgcgtctgcc cgttgaaggc gagcaccgcg
     7561 atcgcctggt tcgggccccg ctcggccgcg aacgcccggg cggcctcgag cgcgccctcg
     7621 atcgcctcgc cgcgcatgct gttgctcgcg tcgatcacga ggacgaccgc gaactcgccc
     7681 gccccggcct cggacgccgg caggagcgtg agatccgtga ccggctcgcc gttctcccgc
     7741 acggtgacga gcccggggtc gagcgcggcc gcgtccggga gcgagagcag gaacgcccgg
     7801 tccgggaagc gggctccctc gagctcgcga aggcgcaggt cggtcgcgcc ggcggccgcg
     7861 ccggggagcg cggcggcggc gagcagcacg cccgcgaggg cgatcaggcg gcgggcgctc
     7921 ctcatcgtgc ctgccgctgg aagtccgcga cgtgtgcccg cgggtcgctg aacaggccgg
     7981 ccggcagggc gacgccgcgc ttctcgaact tgtgcaggaa cgtcggcctg agaccggtcg
     8041 agcggaggct cccgatcacc acgcggtccg aggtcacctg ctcgaccttg aactcgaaga
     8101 tgtcctggag cgtgatcacc tcggactcca tccgcccgac ctcggtgatc gcggtcacgc
     8161 ggcgggagcc gtcctcgagc cgctccaggt ggatgatcat gtcgagggcc gacgccacct
     8221 gctggcggat cgcgcggacg ggcagctcgt agcccgccat cagcaccatc gtctccacgc
     8281 gcgcgagcgc gtcgcggggg gcgttggcgt ggacggtgca gagcgagccg tcgtggcccg
     8341 tgttcatcgc ctggagcatg tcgagggcct ccgcgccgcg gacctcgccg acgatgatcc
     8401 ggtcgggccg catgcggagg gagttccgga cgagctcgcg gatcgggatc tcgccctcgc
     8461 cctcgatgtt cttcggccgc gcctcgagcc tgagcacatg ccgctggttc agccgcagct
     8521 ccgccgcatc ctcgatcgtc acgatccggt ctgcgtcggg gatcgcggtc gagagcgcgt
     8581 tcaggagcgt cgtcttcccc gagccggtgc cgccggagat gaggatgttg aggccggcga
     8641 ggatgcaccg ctggaggaac tcgaccgtct cggtcgagag cgtcccgagc cgcacgaggt
     8701 cggtcaggtc gagccgctgc ttcgagaact tccgcacagt gacgagcggg ccgctcagag
     8761 agagcggcgg gatgacggca ttcacgcggc tgccgtccgg caggcgcgcg tccaccatcg
     8821 gcgaggactc gtcgatccgg cggccgacct gcgcgaccat cttgttgatg atccggcgca
     8881 ggtgcgactc gtcgatgaac cggacggtcg tctcgtagag gcggccctgg cgctcgaccc
     8941 agacgtcgaa cgggccgttc accatgatct ccgtcacgct ctcgtcggcg aggagccgtt
     9001 cgagcgggcc gtgcccgagg atgtcgtccg cgatctcggc ctcgagccgg tcgcggtcgt
     9061 cccgcgagat gccctgctcc ttggcgaggt gcgtgcggat gtccgcggtc acgcggtccc
     9121 ggatcgcgtt cgggtccatg ctcacgttga agagctgggg cccgagctcg ccgatcacgg
     9181 ccaggtggag ccggttcttg agctccgaga acggatcgcg cccgttcgtc ggcgcgaccg
     9241 gccgggtggt ggtcgaaagg cgctcgtgga ggtccatcag acgctcgcct tcttccgccg
     9301 gccgaagcgg cgccgcttgc cgccgttcgc gtcgcgggtc tcgtacagcg ccgcaagctg
     9361 cccgaacgcc ttcgcggcgt cggagcggcg gccggcctgg acgatcggcg tcccctcgtt
     9421 gaccgagcgc gggatgtcgc ggtcgctcgg gaccagcacg tccggcgtgc ggcccacgat
     9481 cgcgacgacg tcgtcgcgcg tgatcccgac gcggctgtcg gcccggttca ggacgaaccg
     9541 cgtccgctcc cgcgagtagc ccatgagctc gagcgtctcg agcccgagct tcgtgttctt
     9601 cagcgagagc gagtccagca tccccacgac gcacacgtgc gaggagctgt cgatcgacgc
     9661 gatcacctcg ggcgtgaacc cgggcggcgt gtcgacgacc acgaagtcgt gcgaggcacg
     9721 aagcgccgcg tagacctcgc ggaggaactc cgtcgtgatt gcccccgcat ggtcgggccg
     9781 ggtcggcgcg acgagcacgc ggacgccgga ggggtgctcg gcgaggtagc cggccacctt
     9841 gtcggcgtcg agcgagccgc ccgacttcgc gaggtcgtag atcgtccgct ccggcgcgag
     9901 gccgagcgag agcgcgacgt cgccgaactg gaggtcgagg tcgacgatcg ccacccggtt
     9961 ccccgcgccc gcgagcgcga ccccgaggtt gcaggcggtg agcgtcttcc ccgtaccgcc
    10021 cttcgggccg aggacgcaga tcatcggggc gagcgcgacg ccgccggcgc cggcggtgcc
    10081 ctgcttgcgg accatcgcct tctcgaacgt gaacgcgatc tgctcggggt tcgcggggag
    10141 gctgacgatg tcgtccgccc cgacctcgaa cacccgcttc acgaagccgt tcggcgagcc
    10201 gtccgagagg acgaccaccg gccgctccgg ccgctgcttg accgcgccgt cgatgagggt
    10261 gatcgcccgg tccgagtagt cgccgcagac gaccgcgagc aggtccgtcg ggacctcctg
    10321 gagcgtctgc cagctctcct gcaggccgtc gatcacgccc acgatctgga cgccgttgtc
    10381 gctcgggagc gaggcgttca cgagctcgct ctccacgccg tcgtcgacgg cgacgaggat
    10441 cctgatcggc gcgctcacgg cttcgctccc agcgacttcg ccgccgccac gggcggcacg
    10501 ccgaagagga cggtctcgag cgtgacgatg tcgggcgtcg tcggctcggc gccggccggc
    10561 gggcgcagga cgatccagac gacgccgttg tcgctcgaga acgcgaccct ggcggcgtcc
    10621 gcctcgctca ggcgaagcgt gacggcctgg ttctgcgcgc cgcccaccgc cgccccggcg
    10681 cccttcggcg cgtcgagcac gacgacgtcc tcgacgatga gcttgaggac gggcctgtcc
    10741 atcgcgttcg ggtccggcga gccgttcgga agcaccttgc ggacgttgaa gcccgcgtac
    10801 acgtcgacgt gatcgccggc cgagacgttc ccgatcatcc cgtgcgcgtt gtcgatcggc
    10861 acggcgatgc cgcgctcgtc ggcgctgatc cgtgccgcga cggtgtccgc gccggcgccg
    10921 ctgaaggcgg aggccgtcag ctgctcgccg ggatagacgt cgtccacggc gacggttccg
    10981 cgcagcgcgg cggggtcggt gatcgcaccc tccttcacct cgctccgcgg ggtggtgacc
    11041 gcctggaaca ggccgtcgga gccgaccatg ttcccgggcg tccccttctc gatcagatcc
    11101 ttcgcgacga ggacggtcat cggctgcgtg gccgtgccga cgctctcgcg atagcgattg
    11161 aggtagacca ggatgatggc cgccgccacg aggccggcaa ccgcgctgat cgcgatcgtg
    11221 cctcctcggg tcgacaacat tctgtgcgcg agttgcacct cgatccctcc tttattcggt
    11281 catgacggcg atgcagctgc cgaacgcgtc gaagtcgcag agctggccgc tgccaccgcc
    11341 cgagttgagc gagaacacgt acttgatgaa ccgcccgacg atgtagcccc ggtcgggcac
    11401 cgggtcgtcg ccgctgcacg ggctcgagaa ccagcccgtg acgtagaacg cggcgaagtt
    11461 cttcacgggc acgacgtcgt tgccgctgcc ctcgaacgtc ccgaacggcg tgacgaacac
    11521 cggcacgatg cgcgggtcgc ccggcgggag gtccgggaag ctcgcccagt tgttcgggct
    11581 cgtgcactgg gcggccccgc cgaggatccg gtcgcggagc ccctgctcga cctggccgat
    11641 cgcaccgccg gtctgcaccg cggtgcactg ccagggctgg gcgctcgccc agagcgtcgg
    11701 cgcggtcgcc gggcaggtgc tcccgctgtt gatctggtac tgcggggcgc acccgtagcg
    11761 gatctcctcg cggatgttcg gcagcgcggg gtcgcagtcg acggtctggt tctggctgcc
    11821 gctcacccgc aggaggatgg gcgggtcgtt cacgctcgtc gcgtggacga ggttccccgc
    11881 gaccgcgatc cgcacgacga ggttcgggct cgtcccgagc tcgaaggagt tcgccccggc
    11941 gacgccgccc tccgagacct cggcgacctt gatcgccccg ctccgcgcct cgctccccgc
    12001 gaacgtccgc tggacgacgc cgagcgtgcc gacgcacttg ttcccgttcg gcttgcagtc
    12061 ctcgagccgg ccgccgacgt tcacctgccc gatcgtctgc tcccagcgca gctcgacggg
    12121 caggggcccg gcggccggcg cgacggagaa gaagtccgtt ccggtcgaaa cccagcgccc
    12181 gctcgccgag tcgtacgtga gcggacgcgt gacgccgccg acgaccgcgg tcaccgacgc
    12241 gccgttcgcg ccgaccgggt cgttcgtccc gaagtcgacg accgcgctca cgccgaccga
    12301 gcacgagctc gcctcggtcg tgaagtacgc gtccgggcac gagccgggaa agagccggac
    12361 gtcgcgggcg atcggcgggt tcggctgcgc gccgctcccg gccgacgacc agccgcggac
    12421 gtagaggagc ccgttcggcc cgccgaggtc gtagcaggcg acgaagcgcc cgccgcagtc
    12481 ggtcgagctg ccgccgccga gcgcgatcct gacgccgatg cgctcggcgc tcaccgggac
    12541 cgagaggggc gccgcggagt tgtcccagac cgtcgtgccg ccggtcgtcg ccgtcttcgc
    12601 gagcggcgtc gaggcgagca cggcgccggt cgcctcgttc acgaagaacg cgcgcgcgac
    12661 gttcggctcg acgtccggca cggcgacggg aagcgcgccg gcggtctgga agagcccctg
    12721 gatctccacc cgcgcgcgcg cgttgatcgc cggcaccgtc gcgatgcgga agtaccaggg
    12781 caggttcgac tcggtgatct tcacgtcgac catccgcgcc gagcacggcg ggccgccgtc
    12841 gctgtagtcc gtgccgccct cgttccagaa gtcctcggag ttgatgagaa cgtgcacgtt
    12901 cgcgccgtcc gtaggcggaa tctgcgtgtt gtacggggtc gagttgcccg gatcgccggc
    12961 gtactggcgc gcgcgctcct cgatcgtgtc gttcgagcac ggccaggtga aagagccgcc
    13021 gccggcgagc gcgcccgcgt ccgcctgaag ctggaggtgg cgcttgtgca cgaaccagtt
    13081 cccgacgtcg acgacgaaga cgacgaagag gacgaccgcc gggatgaaga ggacgaccat
    13141 gacgagcacc ccgccgtcct cacggcgccg gccgcgcagc gcgagctcga ggacgcggct
    13201 cacgagcacc ccgccccgta gacgctcggc gcggcctcga gccgcatcgt cgccgacgcc
    13261 gtcatcgtcg tctgcgcgat gccgagccgc gagccgagga acggaagcca gttgtacgtc
    13321 gtgctcgccg tcacgcgaac ggggtcgccg acctggctcg tcccgttcgg gaaggtgatg
    13381 cagatctgga tcgggcccgc gacggacgcc gtgccgccgt cgcggagctc gggcgtgttc
    13441 gcctgcgagc gcacgtactg ctggagcgac ccgccgcccg ggttcttgtt caccgcggcc
    13501 caccgcgcac cctcgttcgc gaggtgcgtc tggtcgatcc agtagttgaa cgccttgccg
    13561 aagtcgagca tcccgagcag caggacgagg aggaccggca ggacgagggc gaactcgacc
    13621 agggccgcgc cctcctcggg attgcggccg gcgaaacggc tggggagcaa cgaccgcacc
    13681 gtcctagaac gcgctcaccg cgccctgaat ggccgcgtcc acggtcgtgc cgagcagccc
    13741 gagcgtcgcg gtgcaggcga tcgcgacgaa cgcgatgatg agcgcgtact cgacgagcgc
    13801 ctggccgtcc tcgtgcgagg cgttcgacct gttgacgatg tttccgagca aggtgaccct
    13861 ccttcgaagt gcgtggggcc ggcgccgacc cgtgcgggcc ggcgccggct cccggttccg
    13921 tgtgacgcga cgcggctaga ggttgccgac gatggtctgg aggatcccgt cgatccccgt
    13981 gcccagagcg ccgagcgtcg cgacgcacgc cacggacacg agggccagga tcagcccgta
    14041 ctcgacgagg gcctggccct cctcgcggtt ccgaagtgcg ttgaagaact cgatcatgtg
    14101 cgattcacct ccctccctcc gacccggctt gccggcggac gcgccgcgcc cgcggaatgg
    14161 acgccgggcc ggctcgccgg ctcgcgcgtt ccattcggtg aggcggtgcg ttgccacgtg
    14221 cgacgaccgt agatcgcgcc tcgcgccgcc aggcgacctg atcgggtagt tcggcccata
    14281 ccttcgtgcg gcggcacggc ggggggcgat cagcagagtc agttccccat ggacgcgcga
    14341 acggggaaga gacgatgggc ggatgcggga ggcgggcgca gggcgctcgc ctccgcgctc
    14401 ctcgccgttg gatcggcgct tctcgcgctc gtgcgcttcg cgagctgggc cgcgccggat
    14461 cgtgcgcggc ccgggggaac cggccccgcg actgcggagc ccggcccgtc gccggaggcg
    14521 cgcgagccgg aggccgccga cgccgagcgc gacgacacgc gccggaccgc cgcctcgccc
    14581 tcgcggagcc gccggctcgc gagctcactc gtcttcgcgg ccctcttctt ctccggcgcg
    14641 gcgctctccg ccgtcgcggg cgacacgatc ggctcgcccg agccggacgg cccgcgggcg
    14701 gtgacctgca cgggaccggc cgcggacgcc ggcgaggacg cacccgaggg cgagagcccc
    14761 ggcgatccct gcgagaccgg cgagacggcg accgagacga ctcccgagcc ggaggcccct
    14821 ccggcagccg gggacaccgg agagacgccg cccgcgagcg acgcggacgc gggcgcggag
    14881 cctccggccg agattcccgc cgagagcgag ggcggcggcg acgtgccgcc accgacgccg
    14941 gccccggcgc cccacgcgca cgagcattcc gcaggctccg gcgcgccgcc ggtcctcggg
    15001 ggcgcgagcc ccgccgcccc gagggcgctc gaccccgagg cttcggcgga cgactcgatc
    15061 gtctggctcc atcgcgtgct gcccgacccg acgccgccgg cgcggcggct cgcgccggcc
    15121 ttcgcccggg agctccggcg cacggccgcg gagcaccgga tcggctggag cctcctcctc
    15181 gcggtcgtgc gcgcacacgg gaacaccggg cgcgtgcccg tcgggcccgg cacgctcgac
    15241 cgcatcgccg gccgcctcgc ggccgagacg gacgcgctcg gctcgcggaa cgcctggcac
    15301 gtcgcgctcc gctactcggg ccggacggcc ttcgcggacc ggacgctcgc gctcgaccgc
    15361 tacaaccgcg gcgtcggcct ccgcgcgctc gtcaccggcc tcgaggccgc gaaagacgac
    15421 ctggcgcgcc gcgtcctcgc ggatcccgcg atcgagatct acacggccgg gcgcgccgac
    15481 gtcgccgcgg ggcggaccga cgtccggatc ctcgcgctgc tccgctacct ccggatcgcg
    15541 cacggaagcg tcaccgtctc gagcctcacg agcggg
//
LOCUS       DASTFC010000024         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_857345,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000024 DASTFC010000000
VERSION     DASTFC010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857345"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="mutM"
                     /locus_tag="VFO88_00745"
     CDS             <1..618
                     /gene="mutM"
                     /locus_tag="VFO88_00745"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET9544080.1"
                     /translation="HLRMTGSFQHPVAEGPDPHRRAVVTLDDGSDVAYRDVRRFGTWI
                     LLEPGELEAYLDERIGGEPLERAFTTARFARALEGRRAPIKGALLDQRAVAGVGNIYA
                     DEALWLAGIHPERPAGELDAEEVAALRRAIRRVLRLGIERQGATLRDYRDANGRAGAM
                     QHEFRVYGRGGEPCERCGTPIAKTRVAGRGTWFCPSCQAPPSGRS"
     gene            complement(615..866)
                     /locus_tag="VFO88_00750"
     CDS             complement(615..866)
                     /locus_tag="VFO88_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET9544081.1"
                     /translation="MARIELYTTAWCGYCVRAKSLLEQRGLPFEEIRVDSDPHFRTRL
                     QELTGGWTVPQIVVDGEPIGGYVELVALDRNGDLARLAA"
     gene            934..1680
                     /gene="recO"
                     /locus_tag="VFO88_00755"
     CDS             934..1680
                     /gene="recO"
                     /locus_tag="VFO88_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET9544082.1"
                     /translation="MDARTWKTEAVVLRSIRFGEADRVLHLFTLERGRVGAIAKGARK
                     TTSRFGGRLEPFSHVELVLRQGRGDLALVTAAHLVRSHDASRIDPYRMGVGHIGLEAV
                     LKLFVEGDASSRAFHGLARFLDLLDATASALPARPALDPLVLSFQLKLLWLSGYLPHL
                     AGCAACGSEAGLVGFSPAAGGGVCAACEAGSRAISPEGFAGIQALLERPLSEAQAVGL
                     SERGRRDALAVVESSYEYHGGFRLRTTARA"
     gene            1838..>2299
                     /locus_tag="VFO88_00760"
     CDS             1838..>2299
                     /locus_tag="VFO88_00760"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET9544083.1"
                     /translation="MSAPTYQETILRLERFWADYGCVVQQPYHTEVGAGTFNPATFLR
                     CLGPEPWKAAYVEPSLRPSDGRYGENPYRFQQFWQYQVVLKPAPEDVMDVYLRSLEAL
                     GIDPVRHDVRFVEDDWEGPTLGAWGLGWEVWIDGLEVTQFTYFQQLGGLDVD"
BASE COUNT          307 a          815 c          837 g          340 t
ORIGIN      
        1 catctgcgga tgacgggaag cttccagcac ccggtcgcgg agggccccga tccgcaccgg
       61 cgtgcagttg tcacattaga cgacggctcg gacgtcgcgt accgggacgt ccggcggttc
      121 ggcacgtgga tcctgctcga gccgggtgag ctcgaggcct atctcgacga gcgaatcggc
      181 ggggagccgc tcgagcgggc gttcacgaca gcccggttcg cccgcgcgct cgagggccgc
      241 cgggctccga tcaagggcgc gctgctcgac cagcgtgcgg tcgccggcgt cgggaacatc
      301 tacgctgacg aggcgctctg gctcgcaggc atccatccgg agcgccccgc gggcgagctc
      361 gacgcggagg aggtggccgc gctgcggcgt gcgattcgcc gagtgctccg cctcggcatc
      421 gagcgccagg gcgcgacgct cagggattac cgggacgcga acgggcgcgc cggtgcgatg
      481 cagcacgagt tccgcgtgta cggacgaggc ggcgagccgt gcgagcgctg cggaacgccg
      541 atcgcgaaga cccgggtcgc cgggcgcggc acgtggttct gcccctcgtg ccaggcgcca
      601 ccgagcggtc gcagctaggc cgcgagccgg gcgaggtcgc cgtttcggtc gagcgcgacg
      661 agctcgacat agccgccgat cggctctccg tcgacgacga tctgcggcac cgtccacccg
      721 ccggtcagct cctgcagccg cgtccggaag tgcgggtccg agtcgacccg gatctcctcg
      781 aacggcaggc cgcgctgctc gagcaggctc ttcgcacgga cgcagtagcc gcaccaggcg
      841 gtcgtgtaga gctcgatccg ggccatcgct acaagactca ccgtactcgg ccgtgcggaa
      901 accgcgcgcc ggggccgtgt ctacactggc cccgtggacg cccggacctg gaagacggag
      961 gcggtcgtcc tgcgttcgat ccgcttcggc gaggccgacc gggtgctcca cctcttcacg
     1021 ctcgagcgcg ggagggtcgg cgcgatcgcg aagggcgcgc ggaagacgac gtcgcgcttc
     1081 ggcggccgcc tcgagccgtt cagccacgtc gagctggtgc tccggcaggg gcgcggcgat
     1141 ctcgccctcg tgaccgcggc gcatctcgtc cgctcgcacg acgcgagccg gatcgacccg
     1201 taccggatgg gcgtcggcca catcgggctc gaggcggtgc tgaagctctt cgtcgagggc
     1261 gacgcgagct cgcgcgcgtt ccacgggctc gcccggttcc tcgacctgct cgacgccacg
     1321 gcgagcgcgc tcccggcccg gcccgcgctc gacccgctcg tgctctcgtt ccagctcaag
     1381 ctgctgtggc tctccgggta cctgccgcat ctcgcggggt gcgcggcctg cgggagcgag
     1441 gccggcctcg tcggcttctc gccggcggcg ggcggcgggg tgtgtgcggc gtgcgaggcc
     1501 gggtcgcgcg cgatctcacc cgaggggttc gcggggatcc aggcgctgct cgagcggccg
     1561 ctctccgagg cgcaggccgt ggggctctcg gagcgcggcc ggcgcgacgc gctcgccgtg
     1621 gtcgagtcgt cgtacgagta ccacggcggg ttccggctac ggacgaccgc gcgtgcctga
     1681 cgagcgcgtc cgcgatcgac acggactcgt gacagtgcgt cacagacacg tgcgcttttc
     1741 agcaatacga tgaatctcat ggggtggggg cgaaccaccc gttacccgcg tgcgtctatc
     1801 ccagggacgc ggcttccccg ccagtaggat cgggcccgtg agcgccccga cctaccagga
     1861 gacgatcctc cgcctcgagc gtttctgggc ggactacggc tgtgtcgtcc agcagccgta
     1921 ccacaccgag gtcggcgcag gcaccttcaa cccggcgacg ttccttcgct gcctcgggcc
     1981 ggagccctgg aaggccgcgt acgtcgagcc gtcccttcgc ccgtcggacg gacgctacgg
     2041 cgagaacccg taccgcttcc agcagttctg gcagtaccag gtcgtcctca agccggcgcc
     2101 cgaggacgtg atggacgtct acctccgctc gctcgaagcg ctcgggatcg acccggtccg
     2161 gcacgacgtt cgcttcgtgg aggacgactg ggagggcccg acgctcggcg cctggggcct
     2221 cggctgggag gtctggatcg acggcctcga ggtgacccag ttcacgtact tccagcagct
     2281 cggcggtctc gacgtcgac
//
LOCUS       DASTFC010000025        12633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_198099,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000025 DASTFC010000000
VERSION     DASTFC010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12633
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198099"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFO88_00765"
     CDS             <1..771
                     /locus_tag="VFO88_00765"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HET9544084.1"
                     /translation="RLIARNVDVLMDRSPAPEEEAVRRAIEGVSPRVELRRLRLRRAA
                     GLHFADVVIAVPPLAAVGQGHAAADAVEAAVEDALPGTDVVVHVEPAPAQAALVERAH
                     AAALRVPGVREIHNVSVLQAGARTEVSLHLKLPGMLPLAEAHEVASQVEAAIRAELPE
                     TDAVQTHLEPLGGETAAAALAGTDGGRVARVVREHTGAEPRDLRLYRTDAGLVVFLTL
                     GLAPERTLAEAHALASEIEAAVHEAEPSVADVIVHTEP"
     gene            786..926
                     /locus_tag="VFO88_00770"
     CDS             786..926
                     /locus_tag="VFO88_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544085.1"
                     /translation="MRIGRRSAGETRWEKDNRLSRNPAPFIVLALGAALLAGLFLLFW
                     FF"
     gene            complement(952..1350)
                     /gene="grxD"
                     /locus_tag="VFO88_00775"
     CDS             complement(952..1350)
                     /gene="grxD"
                     /locus_tag="VFO88_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET9544086.1"
                     /translation="MALKDRIQETIDSEDVVLFMKGTPEQVLCGNSARALDALRSVGA
                     PVTAVDVLPDPAIRQELSALSNWPTIPQVFVRGELVGGADIVQELAETGELREKLDGA
                     LGEGWQERGREERTVELAEGQGPAGLRLVP"
     gene            complement(1350..1580)
                     /locus_tag="VFO88_00780"
     CDS             complement(1350..1580)
                     /locus_tag="VFO88_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HET9544087.1"
                     /translation="MTVRLEELLRQAFPDATELAVEDRTGGGDHFQVVVASPRLSGLS
                     LVEQHRLVYDALAEPLADGTIHELRIKTRGDG"
     gene            complement(1615..1917)
                     /locus_tag="VFO88_00785"
     CDS             complement(1615..1917)
                     /locus_tag="VFO88_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544088.1"
                     /translation="MEWWIILIVVLVAVAVLLIATVVGRRAQERRTNQQRAEATELRA
                     DAATQAREADERARLAEEEADRARRQRAVAEERAREADELDPDVETDREPERPVDR"
     gene            1996..3111
                     /locus_tag="VFO88_00790"
     CDS             1996..3111
                     /locus_tag="VFO88_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9544089.1"
                     /translation="MNGAARRVEVILSLRTLLLVAGAVALAAALVTIRSAVVLVFFAV
                     FLALVFEYPTRTLMRRLHIGRGIAATTVVLGFVLLFIALMVALVAPLVDDLAVFLRSL
                     PEILESLQESRAFRWLRESGLGDEAEQAARDLAGRIPGTVGGFVGLIGEALTTALVLF
                     TVTFLSLFLVIEMPKIREAVGSVLMPETADRVLGVWEQITRTISRWAIGALVVAIIAG
                     TVQGVTAALLGSSYALALGLIAGLLDLIPNIGATIAAFVLVPVILAEEGPIDALIMLG
                     VIIGYQQVENNLVTPTVMGRATDISAFLVILGVLVFGTLLGVLGALVAVPVTASIQIV
                     LREVTAERRAHVAAAKAEREAAEPTLEPPSAPAPADA"
     gene            3134..3664
                     /locus_tag="VFO88_00795"
     CDS             3134..3664
                     /locus_tag="VFO88_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544090.1"
                     /translation="MLVSLAERPVAARLLGSLLFLLVAGTLALPFVAVSVDERRAEAS
                     GLELAEGEAELTGRYVHAAYEGQAEEVVDEARGPAVALAVAALAGIGLVWLPGRAGLL
                     AGAALAGLALLGLAGIFRTAASSLDLADADLRVGFGLAVIAAAGALAWSSLAASRALF
                     WWRPKPPPARDYFPER"
     gene            complement(3661..3792)
                     /locus_tag="VFO88_00800"
     CDS             complement(3661..3792)
                     /locus_tag="VFO88_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544091.1"
                     /translation="MAIAVLPDGPKSRWNRFVTVLFVVGVAIVLGLLVFSLLDAYVL"
     gene            complement(3825..4247)
                     /locus_tag="VFO88_00805"
     CDS             complement(3825..4247)
                     /locus_tag="VFO88_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9544092.1"
                     /translation="MIEELSHAEIDALLREQVVGRVGCHADGVTYVVPVIYAYDGKAI
                     AVATVEGQKTRMMRANPSVCFEVDAYEGPGRWRSVIVQGTYEELGGDDAQAALGTLAS
                     RFAGGGPARRPGTSGRETVVFRIVVGEATGRAVRRGGR"
     gene            complement(4275..4841)
                     /locus_tag="VFO88_00810"
     CDS             complement(4275..4841)
                     /locus_tag="VFO88_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9544093.1"
                     /translation="MSIEVTEATFEADVVERSHETPVVVDFWADWCQPCHMLAPVLES
                     AVAERDGAVVLAKVDVDANPGLSQRFGIQGIPAVKGFRDGRVVGEFVGVNPAGVTELL
                     ETLTGPSEAERAAAELREAGDLPDVVEALERGDYEAAFERLLAHLDETDADGRDRVRR
                     VMVGLFRDLGAEDPVAARYRRRLASALF"
     gene            complement(4838..5365)
                     /locus_tag="VFO88_00815"
     CDS             complement(4838..5365)
                     /locus_tag="VFO88_00815"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9544094.1"
                     /translation="MSRLHNVDVAALEESAARASADPVTVRQPVELSGEWQVEAGRPQ
                     FRGAIPYPSGEVEFSCDFPPALGGSGAAPNPLAYCLWGGLACYAMTYALEAARLGVEL
                     RALRGTITTEVDQSRALGVSDRPPVEGVAWSLDVDADAPSEVLGRLKEQADERCPGVY
                     CLRNPIPLETRVEAA"
     gene            complement(5362..5652)
                     /locus_tag="VFO88_00820"
     CDS             complement(5362..5652)
                     /locus_tag="VFO88_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9544095.1"
                     /translation="MSRSLSPEDAWKAASRGEVELLDLRTSAERRRYGAPPGARPVSL
                     ARHAVRPEGAGAVYLCQHAARSKLALHRGAAEVAGGFVAWQAAGLPIEAVSG"
     gene            5677..5994
                     /locus_tag="VFO88_00825"
     CDS             5677..5994
                     /locus_tag="VFO88_00825"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9544096.1"
                     /translation="MNAASTPLIPDELLAGVARRFRYLGDPTRLRVLSELHGAGEASV
                     GDVARRSGVPLASVSQHLNRLADGGLVTRRRSGTSVIYAIADPTLERLCEIVCATVRP
                     GGS"
     gene            6004..7311
                     /locus_tag="VFO88_00830"
     CDS             6004..7311
                     /locus_tag="VFO88_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544097.1"
                     /translation="MGPVEDTRRRDTTRWLPLVATGRPAPFRLGGPAYLAAILGWLAA
                     AILFLAAAPDLVATRVSDGTVVAAAHAIGLVVFPFAVAAAVWQLVPVMLRNDPPRAWT
                     RWAALPLLAAGTPLAFALATDRERLAAVAAVLLGAGMLLLLTELAELIRGAPPGRTLV
                     VSRPAVALAAAHAAAAFGLGVAVLAGDGPEPLGLSYERALLVHVSVALVGWLTVLIAA
                     VGRTLVPMLGLAASAPPRRVPAAELAIVSGLWLAVAGLAAPADALLAAGVVVVLAGLA
                     APARTFLRVARTGTIGWREGPVAHVTLGLLFVLQAAGLALAAAAGAVDGRRAAVTVVL
                     LLGVGWAAGTILGHLGKLVSLSAWGSWPPGPRPKQAELYPRRGWQAEVVLFAPGAQLL
                     ALGVLLESTALARAGGALLVAAAAVALACGIETVRRAARGLAG"
     gene            7331..7486
                     /locus_tag="VFO88_00835"
     CDS             7331..7486
                     /locus_tag="VFO88_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544098.1"
                     /translation="MGAADAARKLLEATPIVADEDEYDLFAGLPLTPPAEAEEGNDAV
                     HAEEREP"
     gene            7486..8220
                     /locus_tag="VFO88_00840"
     CDS             7486..8220
                     /locus_tag="VFO88_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HET9544099.1"
                     /translation="MAGEKVPRLLELGSKGRDVRALKRALRRAGHWPKGARVSNVFDE
                     DTDKAVRAFQRATRLEIDGQVGDDTFGALLPHYDAYSKLLVDQVRKRMTKLANPSGKR
                     QRIVAAAFVGYTNRDRIHYTQDARRMQGVREGIRVPRFPEFEDCSSFATWCYWVAGAP
                     DPNGRGYDGFGFTGTQIGPGKQVTEPRPGDLVFYGWEPAPRNCPRHVAVYVGNGKVVS
                     HGSDTGPHLVTMDYRTDRSQIRSYLP"
     gene            complement(8217..9260)
                     /locus_tag="VFO88_00845"
     CDS             complement(8217..9260)
                     /locus_tag="VFO88_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9544100.1"
                     /translation="MAGSDPHATLVRFGRALRAEGLPVGPDRVAELGRAAALLRPGEL
                     YWAGRATLVSRPEQIETYDAVFGRFFGPPTPTPGPPLRLRVEAEVEEDVGLASRAETL
                     REKSFAQCSREELSELAELMGRIELVVPRRRTRRREPARSGTPDLRRTLRRSFRTGGD
                     PLERAWRRRRPRARRLILLLDVSGSMDAYSRALVLFAHAALRSDRRFEAFCFGTRLTR
                     VTRQLEGSDPDEALRRAAAEVLDWDGGTRIGECLQRFLDEHGHGGLARGAVVVLCSDG
                     LDVGEPELLAEQMARLSRLAYRVVWLNPLKGDPAYAPLARGMQAALPYVDLFASGHNL
                     ASLEEVGRALARR"
     gene            complement(9264..10130)
                     /locus_tag="VFO88_00850"
     CDS             complement(9264..10130)
                     /locus_tag="VFO88_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9544101.1"
                     /translation="MRFGSAREVEEALRKESYLPDRGLATAVYLALAMRRPLLLEGEA
                     GVGKTEVGKTLARLLGADLVRLQCYEGIDASQALYEWDYARQLLYARALQAGEVSVDE
                     RVAELYGPDFLVERPVLRALRAGAGAVLLVDELDRADDEFEAFLLEVLSDFTVTVPEL
                     GTIAADEPPVVVITSNRTRELHDALKRRCLYHWIEFPDVEREAQIIRLRVPGVEEALA
                     RSVAETVARLRALDLVKRPGAAEAIDWAAALTLLGAPAAEGEAARETLGAAVKNREDQ
                     ARVEAAWAELVE"
     gene            10237..11112
                     /locus_tag="VFO88_00855"
     CDS             10237..11112
                     /locus_tag="VFO88_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9544102.1"
                     /translation="MYPARFEYHAPASVDEALALLERYGDEGKVLAGGQSLIPLMKLR
                     FASPGALVDVNRIQGLDTLEEGPEGLTIGPLVRHRDCERSATLRGRYGVLADAAPQIS
                     DPIVRNLGTVAGSLAHADPQGDWGSALLAAGAEVEATGPNGTRTIALDDLFQGPFTTS
                     LEPTELVTAVRVPDPGPRAGGTYLKLERKVGDFATVGVAVHVSFANGTVGRAGIALTA
                     VGPRSIRATAAEEALAGATLDDDTIRHAAELAAQAAEPRSDHRGTADYKRSVVRVFTE
                     RGLRKAAAAAREEER"
     gene            11109..11612
                     /locus_tag="VFO88_00860"
     CDS             11109..11612
                     /locus_tag="VFO88_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9544103.1"
                     /translation="MTVAAETGSLTRAITVTVNGEARSAEVENRLLLVHFLRQTLGLT
                     GTHVGCDTTSCGACTVLLDGTPVKSCTVLAVSVEGREVTTVEGLAPAGGLHPVQEGFH
                     EEHGLQCGFCTPGMMLASAALLEENPDPSEEEIRWALSGNICRCTGYQNIVKAVQHAG
                     AKMREES"
     gene            11615..>12633
                     /locus_tag="VFO88_00865"
     CDS             11615..>12633
                     /locus_tag="VFO88_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9544104.1"
                     /translation="MAVSEDQVIRGIGHSVKRFEDGRFIRGRGNYVDDIVLPRMLHME
                     FLRSPLAHARIRSIDTSAAEAVPGVVAVVTGELLAQHNLAWMPTLSGDTQAVLATDKV
                     RFQGQEVAAVIAESAYVAKDATELIDVDYEPLPPITSPQQALAEGAPVIRDDKEGQDS
                     NHIYSWEAGDAAATEQAFAEADRVVELETFYPRCHPAPLECCGCVADVDPATGKATIY
                     MTSQAPHAHRTLFALVAGLPEHKIRIVSPDIGGGFGNKVPIYPGYVVATAASLLLGRP
                     VKWTEDRTGNLISTGFARDYHMRGELAVKDGRMTGLRVNMLSDNGAFFADAQPTRCKA
                     GLFHIV"
BASE COUNT         1564 a         4801 c         4616 g         1652 t
ORIGIN      
        1 cgcctgatcg cccgcaacgt cgacgtcctc atggaccgga gccccgcgcc cgaggaggag
       61 gcggtgcgac gcgcgatcga gggcgtctcg ccgcgcgtcg agctgcgccg cctccggctc
      121 cggcgcgcgg cggggctcca cttcgcagac gtcgtgatcg ccgtcccgcc gctcgccgcg
      181 gtcgggcagg ggcacgccgc ggcggacgcg gtcgaggcag ccgtcgagga cgcgcttccc
      241 ggaaccgacg tggtcgtcca cgtcgagccc gcgccggcgc aggcggcgct cgtcgagcgc
      301 gcgcacgccg ccgccctccg cgtgccgggc gtgcgcgaga tccacaacgt ctccgtgctc
      361 caggcggggg cgcggacgga ggtctcgctc catctgaagc tccccgggat gctcccgctc
      421 gccgaggcgc acgaggtcgc gtcgcaggtc gaggcggcca tccgcgccga gctgccggag
      481 acggacgccg tgcagacgca cctcgagccg ctcggcggcg agacggcggc ggccgccctc
      541 gccgggacgg acggcgggcg agtcgcgcgg gtcgtccgcg agcacaccgg cgcggagccg
      601 cgcgacctgc gcctctaccg caccgacgcg ggcctcgtcg tcttcctcac gctcggcctc
      661 gcgcccgagc gcacgctcgc cgaggcgcac gcgctcgcga gcgagatcga ggcggccgtg
      721 cacgaggcgg agcccagcgt cgcagacgtc atcgtccaca ccgagccctg aatagactcc
      781 gcccgatgcg gatcggacgc cgcagcgcgg gcgagacccg ctgggaaaag gacaaccggc
      841 tctcgagaaa cccggcgccg ttcatcgtgc tcgcgctcgg cgcggcccta ctggccgggc
      901 tcttcctgct cttctggttc ttctaggccg tcccggggcg tgacgccagg ctcacgggac
      961 gagccggagc cccgccggcc cctgcccctc cgccagctcg accgtccgct cctcgcggcc
     1021 gcgctcctgc catccctcgc cgagcgcgcc gtcgagcttc tcgcgcagct cgcccgtctc
     1081 cgcgagctcc tggacgatgt ccgcgccgcc gacgagctcg ccgcgcacga acacctgcgg
     1141 gatcgtcggc cagttcgaga gggccgagag ctcctggcgg atcgccgggt ccggcagcac
     1201 gtcgaccgcc gtgaccggcg cgccgaccga gcggagcgcg tcgagcgcgc gggccgagtt
     1261 cccgcacagc acctgctccg gggtgccctt catgaagagg acgacgtcct cggagtcgat
     1321 cgtctcctgg atcctgtcct tcagcgccat cagccgtcac ctcgcgtctt gattcgcagc
     1381 tcgtggatgg tgccgtccgc gagcggctcc gccagagcgt cgtagacgag gcggtgctgc
     1441 tcgacgagcg agaggccgga gagccgcggg ctcgccacga ccacctggaa gtggtcgccg
     1501 ccgccggtgc ggtcctcgac cgcgagctcg gtcgcgtccg ggaacgcctg ccggagcagc
     1561 tcttcgagac ggacggtcac gcctcgaagg taccagcgcg cccccgcgcc gggactagcg
     1621 gtcgacgggg cgctcgggct cgcggtcggt ctcgacgtcg gggtcgagct cgtcggcctc
     1681 gcgggcccgc tcctcggcga cggcgcgctg acggcgtgcc cggtcggcct cttcctcggc
     1741 cagccgcgcc cgttcgtcgg cctcgcgcgc ctgtgtggcc gcgtcggcgc ggagctcggt
     1801 cgcctcggcc cgctgctggt tcgtccgccg ctcctgcgcg cgccgcccga cgacggtcgc
     1861 gatcagaagc accgccacgg ccacgagcac gacgatgagg atgatccacc attccacggc
     1921 ggcgcttgtg cccgaacccc gcggggagga aacccggacg gttcaccggc caccggcggg
     1981 cgtacactcg cgcgcatgaa cggagccgcc cggcgcgtcg aggtcatcct ctcgctgcgg
     2041 acgctcctcc ttgtcgcggg cgcggtcgcc ctcgcggcgg cgctcgtgac gatccgctcg
     2101 gcggtcgtcc tcgtcttctt cgcggtcttc ctcgcgctcg tcttcgagta tccgacgcgt
     2161 accctcatgc gccggctcca catcgggcgc ggcatcgcgg cgacgacggt agttctcggc
     2221 ttcgtcctcc tcttcatcgc gctcatggtc gcgctcgtcg cgccgctcgt ggacgatctc
     2281 gccgtcttcc tccgctcgct cccggagatc ctcgagagcc tccaggagag ccgcgcgttc
     2341 cgctggctgc gcgagagcgg gctcggagac gaggccgagc aggcggcgcg cgacctggcc
     2401 ggccggatcc cgggcacggt gggcggcttc gtcgggctca tcggcgaggc gctcacgacg
     2461 gcgctcgtcc tcttcaccgt cacgttcctc tccctgttcc tcgtgatcga gatgccgaag
     2521 atccgcgagg ccgtcggcag cgtcctcatg cccgagacgg cggaccgggt gctcggggtc
     2581 tgggagcaga tcacccgcac gatctcgcgc tgggcgatcg gcgcgctcgt cgtcgcgatc
     2641 atcgcgggga cggtgcaggg cgtgaccgcg gcgctgctcg ggtcgagcta cgccctcgcg
     2701 ctcgggctca tcgccgggct cctcgacctg attccgaaca tcggcgcgac gatcgccgcg
     2761 ttcgtcctcg tcccggtcat cctggccgag gaaggcccga tcgatgcgtt gatcatgctc
     2821 ggcgtgatca tcggctacca gcaggtcgag aacaacctcg tcacgccgac cgtgatgggc
     2881 cgggcgacgg acatctccgc cttcctcgtc atcctcggcg tgctcgtctt cgggacgctc
     2941 ctcggcgtgc tcggcgcgct cgtcgccgtg ccggtgacgg cgtcgatcca gatcgtcctg
     3001 cgcgaggtca cggccgagcg gcgggcgcac gtcgcagcgg cgaaggccga gcgggaagcc
     3061 gccgagccga cgctcgagcc gccttccgcg cccgccccgg cggacgcctg agggcgctcc
     3121 ctacactcgg gtcgtgctcg tcagcctcgc cgagcggccg gtcgcggcgc gcctcctcgg
     3181 ctcgctgctc ttcctcctcg tcgcgggaac gctcgcgctg cccttcgtcg ccgtgtccgt
     3241 ggacgagcgg cgcgcggagg cgtccgggct cgagctcgcc gagggcgagg cggagctgac
     3301 cggccgctac gtccacgcgg cctacgaggg gcaggcggag gaggtcgtcg acgaggcgcg
     3361 cgggccggcg gtcgccctcg ccgtcgccgc gctcgcgggg atcgggctcg tctggctccc
     3421 ggggcgcgcc ggcctcctgg ccggggcggc gctcgccggt ctcgcgctgc tcgggctcgc
     3481 cgggatcttc cgcacggcgg cgtcctccct cgacctggcg gacgcggacc tccgcgtggg
     3541 cttcgggctc gccgtgatcg cggcggccgg cgcgctcgcc tggtcttcgc tcgccgcctc
     3601 gcgcgcgctc ttctggtggc ggccgaagcc cccgccggcg cgcgactact ttcccgagcg
     3661 ctagaggacg taggcgtcga ggagcgagaa cacgagcagc ccgaggacga tcgccacgcc
     3721 gacgacgaag aggacggtga cgaagcggtt ccagcggctc ttcggcccgt cggggagcac
     3781 ggcgatggcc acgcggccat tctctgcgat tcgccggaga gtgtctaccg cccgccgcgg
     3841 cgcaccgcgc gaccggtcgc ctcgccgacg acgatccgga agacgaccgt ctcgcgcccg
     3901 ctcgtacccg ggcggcgggc cggcccgccg cccgcgaagc gcgaagcgag cgtcccgagc
     3961 gccgcctgcg cgtcgtcgcc cccgagctcc tcgtacgtcc cctggacgat gacgctgcgc
     4021 cagcgccccg gcccctcgta cgcgtcgacc tcgaagcaga cgctcgggtt cgcgcgcatc
     4081 atccgcgtct tctggccctc gaccgtcgcg accgcgatcg ccttgccgtc gtacgcgtag
     4141 atgaccggga cgacgtacgt caccccgtcc gcgtgacagc cgacgcggcc gacgacctgc
     4201 tcccggagga gcgcgtcgat ctccgcgtgg gagagctcct cgatcacgcg ccggaggcta
     4261 gccggtcagt ccggtcagaa gagcgcgctc gcgagccggc ggcggtagcg cgcggcgacc
     4321 gggtcctcgg cgccgaggtc gcggaagagc ccgaccatca cgcggcggac gcggtcgcgg
     4381 ccgtcggcgt ccgtctcgtc gaggtgcgcg agcagccgct cgaacgcagc ctcgtagtcg
     4441 ccgcgctcga gcgcctcgac cacgtccggg aggtcgccgg cctcgcgcag ctcggcagcg
     4501 gcgcgctccg cctccgacgg gccggtgagc gtctcgagga gctccgtcac gcccgcgggg
     4561 ttcacgccga cgaactcgcc gacgacgcgg ccgtcccgga agcccttcac ggcggggatc
     4621 ccctggatgc cgaagcgctg cgagagcccc gggttcgcgt ccacgtccac cttcgcgagg
     4681 acgaccgcgc cgtcgcgctc ggcgaccgcc gactcgagca ccggcgcgag catgtggcac
     4741 ggctggcacc aatccgccca gaagtcgacc acgacgggcg tctcgtgcga gcgctcgacg
     4801 acgtccgcct cgaacgtcgc ctcggtgacc tcgatgctca cgcggcctcc acgcgggtct
     4861 cgagcgggat cgggttgcgg agacagtaga cgccggggca gcgctcgtcc gcctgctcct
     4921 tcagccggcc gagcacctcg ctcggcgcgt cggcatcgac gtcgagcgac caggcgactc
     4981 cctcgaccgg cggccggtcg gagacgccga gcgcccgcga ctggtcgacc tcggtcgtga
     5041 tcgtgccgcg cagcgcgcgc agctcgacgc cgaggcgcgc cgcctcgagc gcgtacgtca
     5101 tcgcgtagca ggcgagcccg ccccagaggc agtacgcgag cgggttcggt gccgcgccgc
     5161 ttccgccgag cgccggcggg aagtcgcacg agaactcgac ctcgccgctc gggtacggga
     5221 tcgcgccgcg gaactgcggc cggcccgcct cgacctgcca ttcgccggag agctcgaccg
     5281 gctggcgaac cgtgaccggg tcggcggagg cgcgcgcggc cgactcctcg agcgcggcga
     5341 cgtcgacgtt gtggaggcgg ctcacccgga caccgcctcg atcgggagcc cggccgcctg
     5401 ccaggcgacg aagccgccgg ccacctccgc cgcgccgcgg tgcagggcga gcttcgagcg
     5461 ggcggcgtgc tggcagaggt agacggcgcc ggcgccctcg ggccgcacgg cgtgacgggc
     5521 gagcgagacg ggccgcgcgc cgggcggggc cccgtagcgg cggcgctcgg cgctcgtgcg
     5581 caggtcgagc agctcgacct cgccgcggct cgcggccttc caggcgtctt cggggctcag
     5641 cgatctcgac atccgttcca ctattctatt gatgagttga acgctgcgtc gaccccactg
     5701 attccggacg agctgctcgc cggcgtcgcc cgccgcttcc gctacctcgg cgaccccacg
     5761 cggctgcgcg tcctcagcga gctgcacggg gcgggggagg cgtcggtcgg cgacgtcgcc
     5821 cgccgctcgg gcgtcccgct cgcgagcgtc tcgcagcacc tgaaccgcct cgcggacggc
     5881 ggcctcgtca cgaggcggcg ctcggggacg agcgtcatct acgcgatcgc cgacccgacg
     5941 ctcgagcggc tctgcgagat cgtctgcgcg accgtccggc ccggcggttc ataacctggg
     6001 ggcgtgggcc ccgtcgagga cacgcgccgg agggacacga cgcgctggct cccgctggtc
     6061 gcgaccggcc gcccggcgcc gttccgcctc ggcgggcccg cgtacctcgc cgccatcctc
     6121 gggtggctcg cggcggcgat ccttttcctc gcggcggcgc cggacctcgt ggcgacccgg
     6181 gtctccgacg ggacggtcgt ggccgcggcg cacgcgatcg ggctcgtcgt cttcccgttc
     6241 gcggtcgcgg cggccgtctg gcagctcgtc ccagtgatgc tccggaacga cccgccgcgg
     6301 gcgtggaccc gctgggccgc gctcccgctt ctcgcggcgg gcacgccgct cgcgttcgcg
     6361 ctcgcgaccg accgggagcg gctcgcggcc gtcgccgccg tgctcctcgg cgccgggatg
     6421 cttctccttc ttacggagct cgccgagctc atccggggcg cgccgcccgg ccggacgctc
     6481 gtcgtcagcc gccccgcggt cgcgctcgcc gccgcccacg cggcggccgc gttcggcctc
     6541 ggggtcgcgg tgctcgcggg tgacgggccc gagccgctcg ggctctcgta cgagcgcgcg
     6601 ctcctcgtcc acgtctcggt cgcgctcgtc ggctggctca ccgtgctcat cgccgccgtc
     6661 gggcggacgc tcgtgccgat gctcggcctc gccgcctccg cgccgccgcg gcgcgtgccg
     6721 gccgcggagc tcgcgatcgt ctcgggcctc tggctcgccg tcgccggcct ggccgcgccc
     6781 gccgacgcgc tcctcgcggc cggcgtggtc gtggtgctcg ccgggctcgc cgcgcccgcg
     6841 cggacgttcc tccgcgtcgc gcgcaccggg acgatcggct ggcgcgaggg cccggtcgcg
     6901 cacgtgacgc tcgggctcct gttcgtcctc caggcggctg ggctcgctct cgccgccgcg
     6961 gccggcgccg tcgacggccg ccgcgccgcc gtcacggtcg tgctcctgct gggcgtcggc
     7021 tgggcggcgg ggacgatcct cgggcacctg gggaagctcg tctcgctctc ggcgtggggg
     7081 tcgtggccgc ctgggccgcg cccgaagcag gcggagctct acccgcgccg cggctggcag
     7141 gccgaggtcg tcctcttcgc gccgggcgcc cagctcctcg cgctcggcgt gctcctcgag
     7201 tcgacggcgc tcgcgcgggc cggcggagcc ctcctggtcg cggccgccgc ggtcgcgctc
     7261 gcgtgcggaa tcgagaccgt ccggcgcgcc gctcgcggac tggccggata ggcgctacag
     7321 tcgagtcgtc gtgggagccg ccgacgccgc acgaaagttg ctcgaggcca cgccgatcgt
     7381 cgcggacgag gacgagtacg acctcttcgc cggcctcccg ctcaccccac cggcggaggc
     7441 cgaggagggc aacgacgccg tccacgccga ggagcgcgag ccctgatggc gggcgagaag
     7501 gtcccgcgcc tgctcgagct cgggtcgaag ggccgtgacg tccgcgcgct gaagcgcgcc
     7561 ctccgccgcg ccggccactg gccgaagggc gcgcgggtct cgaacgtctt cgacgaggac
     7621 acggacaagg cggtccgcgc cttccagcgg gcgacccgcc tggagatcga cgggcaggtc
     7681 ggggacgaca cgttcggcgc cctgctcccg cactacgacg cgtactcgaa gctcctcgtc
     7741 gaccaggtgc gcaagcgcat gacgaagctc gcgaacccga gcggcaagcg gcagcggatc
     7801 gtcgcggccg cgttcgtcgg gtacacgaac cgcgaccgga tccactacac gcaggacgcg
     7861 cgccgcatgc agggcgtgcg cgaggggatc cgcgtgccgc gcttcccgga gttcgaggac
     7921 tgctcctcgt tcgcgacctg gtgctactgg gtcgccggcg cgcccgaccc gaacggccgc
     7981 ggctacgacg gcttcgggtt caccgggacg cagatcgggc cggggaagca ggtgaccgag
     8041 ccgcgcccgg gcgacctcgt cttctacggc tgggagcccg ctccgcgcaa ctgtccgagg
     8101 cacgtcgcgg tctacgtcgg gaacggcaag gtcgtctccc acgggagcga caccgggccg
     8161 catctcgtga ccatggacta ccgcaccgac cgcagccaga tccgcagcta cctcccctag
     8221 cgccgcgcca acgcgcggcc gacctcctcg aggctcgcga ggttgtggcc gctcgcgaag
     8281 aggtcgacgt acgggagcgc cgcctgcatg ccgcgcgcga gcggcgcgta cgctgggtcg
     8341 cccttcagcg ggttcagcca gacgacgcgg taggcgaggc gcgagagccg ggccatctgc
     8401 tcggcgagga gctccggttc gccgacgtcg agcccgtccg agcagaggac gacgacggcg
     8461 ccgcgtgcga ggccgccgtg gccgtgctcg tcgaggaagc gctggaggca ctcgccgatc
     8521 cgcgtcccgc cgtcccagtc gaggacctcg gcggccgcgc gccgcagcgc ctcgtccgga
     8581 tccgacccct cgagctggcg cgtgacgcgc gtgagccgcg tcccgaagca gaacgcctcg
     8641 aagcgccggt cggagcggag cgcggcgtgg gcgaagagga cgagcgcgcg cgagtacgcg
     8701 tccatcgatc cggacacgtc gaggagcagg atcagccggc gcgcccgtgg gcgccggcgc
     8761 cgccaggcgc gctcgagcgg gtcgccgccc gtgcgaaacg accggcggag cgtccggcgc
     8821 aggtccggag tgcccgagcg ggccggttcg cgccgccgcg tccgtcgccg tggcacgacg
     8881 agctcgatcc ggcccatgag ctcggcgagc tccgagagct cctcgcgcga gcactgcgcg
     8941 aagctcttct cgcgcagggt ctccgcccgc gaggcgagac ccacgtcctc ctcgacctcg
     9001 gcctcgacgc ggagccggag cggcgggccg ggcgtcggcg tcggcgggcc gaagaagcgg
     9061 ccgaacaccg cgtcgtaggt ctcgatctgc tccggccgcg agacgagggt cgcccgcccg
     9121 gcccagtaga gctcgcccgg ccggaggagc gcggcggcgc ggccgagctc cgcgacgcgg
     9181 tcgggcccga cggggagccc ctcggcgcgg agcgcacgcc cgaagcggac gagcgtcgcg
     9241 tgcgggtccg acccggccac gggctactcc acgagctcgg cccaggcggc ctcgacgcgc
     9301 gcctggtcct cgcggttctt cacggccgcg ccgagcgtct cgcgcgcggc ctccccctcg
     9361 gcggcgggcg cgcccaggag ggtcagcgcc gcggcccagt cgatcgcctc cgcggcgccc
     9421 ggccgcttca cgaggtcgag cgcgcggagg cgcgcgaccg tctccgcgac cgagcgcgcg
     9481 agcgcctcct cgacgccggg cacgcggagc cggatgatct gcgcctcgcg ctccacgtcc
     9541 gggaactcga tccagtggta gaggcagcgg cgcttgagcg cgtcgtggag ctcgcgcgtc
     9601 cggttcgacg tgatgacgac gaccggcggc tcgtcggcgg cgatcgtccc gagctcgggc
     9661 acggtgaccg tgaagtcgga tagcacctcg aggaggaacg cctcgaactc gtcgtccgcg
     9721 cggtcgagct cgtccacgag gagcacggcc ccggcgccgg cgcgcagcgc gcgcaggacg
     9781 ggccgctcga cgaggaagtc gggcccgtag agctccgcca cacgctcgtc gacggagacc
     9841 tcgcccgcct ggagcgcccg cgcgtagagg agctggcgcg cgtaatccca ctcgtagagc
     9901 gcctgcgacg cgtcgatccc ctcgtagcac tggagccgca cgaggtcggc gccgagcagc
     9961 cgcgcgagcg tcttcccgac ctccgtcttc ccgacccccg cctcgccctc gaggaggagc
    10021 ggccggcgca tcgcgagcgc gaggtagacc gccgtcgcga gcccccggtc gggcaggtac
    10081 gactccttcc ggagcgcctc ctcgacctcc ctggccgaac cgaaccgcac ggcggaagcg
    10141 tacgggatgg ttgacgtgac aaggaaccct gcgagggcgt atcctcgcag gggctgcacc
    10201 cccacagccc tgcggagcgg gagaaggagg caaccgatgt atcccgcgag gttcgagtac
    10261 cacgctcccg cgagcgtcga cgaggcgctc gccctcctgg agcgctacgg cgacgagggc
    10321 aaggtgctgg ccggcgggca gagcctcatt ccgctcatga agctccgctt cgcctcgccg
    10381 ggcgcgctcg tcgacgtcaa ccgcatccag gggctcgaca cgctggagga ggggccggag
    10441 ggactgacga tcggcccgct cgtccggcac agggactgcg agcgctcggc gacgctgcgc
    10501 gggcgctacg gcgtcctcgc cgacgccgcg ccgcagatct ccgacccgat cgtccgcaac
    10561 ctcgggacgg tcgcgggctc gctcgcgcac gccgatccgc agggcgactg gggctcggcg
    10621 ctcctcgccg cgggcgcgga ggtcgaggcg accggcccga acgggacgcg cacgatcgcg
    10681 ctcgacgatc tcttccaggg gccgttcacg acgagcctcg agccgaccga gctcgtgacc
    10741 gccgtccgcg tgcccgaccc ggggccgcgg gcgggcggga cgtatctcaa gctcgagcgg
    10801 aaggtgggcg acttcgccac cgtcggcgtc gccgtccacg tgtcgtttgc gaacgggacg
    10861 gtcgggcgcg ccgggatcgc gctcacggcc gtcgggccgc gctcgatccg ggcgacggcg
    10921 gccgaggagg cgctcgccgg cgcgacgctc gacgacgaca cgatccgcca cgcggccgag
    10981 ctcgccgcgc aggccgccga gccgcgctcc gaccaccgcg gcaccgccga ctacaagcga
    11041 agcgtcgttc gggtcttcac ggagcgaggg ctccggaagg cggccgcggc ggcgcgggag
    11101 gaggaacgat gaccgtcgcc gccgagacgg gctcgctcac gcgggcgatc accgtcaccg
    11161 tgaacgggga ggcgcgctcc gccgaggtgg agaaccggct cctgctcgtc cacttcctgc
    11221 gccagacgct cggcctcacg ggcacgcacg tcgggtgtga cacgacgagc tgcggcgcgt
    11281 gcaccgtcct cctcgacggg acccctgtga agtcgtgcac cgtcctcgcc gtgtcggtcg
    11341 agggacgcga ggtgacgacg gtcgagggcc tcgccccggc gggcgggctc cacccggtgc
    11401 aggagggctt ccacgaggag cacgggctcc agtgcggctt ctgtacgccg gggatgatgc
    11461 tcgcctcggc cgcgctcctc gaggagaacc cggacccgtc cgaggaggag atccgctggg
    11521 cgctctccgg gaacatctgc cggtgcaccg gctaccagaa catcgtcaag gccgttcagc
    11581 acgcgggcgc gaagatgcgg gaggagagct agccatggcc gtcagcgaag atcaggtcat
    11641 ccgcgggatc ggccactccg tcaagcgctt cgaggacggg cgcttcatcc gcggtcgggg
    11701 gaactacgtg gacgacatcg tcctgccccg gatgctccac atggagttcc tgcgcagccc
    11761 gctcgcgcac gcacgcatcc gctcgatcga cacgtcggcc gccgaggcgg tgccgggcgt
    11821 cgtcgccgtc gtgaccggcg agctgctcgc gcagcacaac ctggcctgga tgccgacgct
    11881 ctcgggcgac acgcaggccg tgctcgcgac ggacaaggtg cgcttccagg gccaggaggt
    11941 cgccgcggtg atcgccgagt ccgcgtacgt cgcgaaggac gcgaccgagc tcatcgacgt
    12001 cgactacgag ccgctgccgc cgatcacgag cccgcagcag gcgctcgccg agggcgcgcc
    12061 cgtgatccgc gacgacaagg aggggcagga ctcgaaccac atctacagct gggaggccgg
    12121 cgacgccgcg gcgaccgagc aggcgttcgc ggaggccgac cgggtggtcg agctcgagac
    12181 gttctacccg cgctgccacc cggcgccgct cgagtgctgc gggtgcgtcg cggacgtcga
    12241 cccggcgacc ggcaaggcga cgatctacat gacctcgcag gccccgcacg cccaccggac
    12301 gctcttcgcg ctcgtcgccg ggctgccgga gcacaagatc cggatcgtct cgccggacat
    12361 cggcggcggc ttcgggaaca aggttccgat ctaccccggc tacgtcgtcg cgaccgccgc
    12421 ctccctgctc ctcgggcggc cggtgaagtg gaccgaggac cgcacgggca acctcatctc
    12481 gaccggcttc gcccgcgatt accacatgcg gggcgagctg gccgtgaagg acgggcggat
    12541 gacggggctc cgcgtgaaca tgctctcgga caacggcgcg ttcttcgcgg acgcgcagcc
    12601 gacgaggtgc aaggcggggc tcttccacat cgt
//
LOCUS       DASTFC010000026         4873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_840934,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000026 DASTFC010000000
VERSION     DASTFC010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840934"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFO88_00870"
     CDS             complement(<1..603)
                     /locus_tag="VFO88_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9544105.1"
                     /translation="MSLSYDLSDEHELIRTTVREFAEQRIAPVAEELDREHRFPYDIV
                     SELAELGLMGIPIPEEYEGAGSDTLAYAIAIEELTRVDSSVAITVAAHTSLGTMPVFL
                     YGTEEQKREWLPDLASGRRLGAFGLTEPGAGSDAGATQTRAELRDGQWVVNGSKIFIT
                     NAGTDITWGVTITARTGEDEISNVVVPNGTSGYEISPPMEK"
     gene            complement(600..2102)
                     /locus_tag="VFO88_00875"
     CDS             complement(600..2102)
                     /locus_tag="VFO88_00875"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET9544106.1"
                     /translation="MDTAALSTPASRVEADVLGVALAEPPALSPAARELDEALGGRLG
                     RAVSDGDATGRRGTVTVLDTDGEGAPRRVALVGLGAGRDLDADAVRAAACRVAGAAGE
                     APGRTVAWLLDGETTGLETAAEARAVVDGVALASHDTGRWKRDPADEERVERLVLVGK
                     RAAEAEEAARTEGVVAGWQNHARDLVNAPANELTPAALADAAVAVADRFDAIDAEVIG
                     PDELAAAGMGALAAVAQGSHNPGRLVTLRYEPAGAEETLVLGLVGKGITFDTGGISLK
                     PADALEEMKSDMAGAAATLAGLAAIAELGLSLRIVAVVAAAENMPAGHAYRPGDIVRA
                     ADGTTIEVTNTDAEGRLVLADALLHARAQGATHLLDLATLTGTIQVALGDLYAGLFGN
                     NEAWLAAVLEAAERSGDHVWPMPLHPAYARFLDSQFADLKNTPDKKRGSSSIGAAFLE
                     RFAGEGPWAHLDIAGTAYLDRGRDYYPRPGATGFGVRLIAELATALTRDR"
     gene            2338..4578
                     /locus_tag="VFO88_00880"
     CDS             2338..4578
                     /locus_tag="VFO88_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544107.1"
                     /translation="MTSEAEPGPAGVTASALGLPRYSVALVQSELEMLNTPGNELSAF
                     LLADPGRWRTEVFTERTFEAALGSANRFDCVILGFNAVYQSDAIQEALADWTDVTGLV
                     VLHQLKQRGLSFLPPDLALTLNELREEARGAVVHRHTRPQDEILLNWPEPVAEPAAKL
                     GTVTSAVMCHLETLEAGTWRTSLELEVGTRRLPVLLRTSSLRRPRVIVCTLQLEPRKP
                     LHAALLRNMITYCAAGWPEIAIATEERSGEGADIARKLRVQGANAVELGIDEPGRLHF
                     DRWPLRGVHEVVVDERWAAGDVLGSVEAREWLEVGNTLVRVGSGGLTFHHGASDAHLV
                     GERWATWFHGIDHATWHGGATRDGGEEPGSIFGTRAVFRVLELLARDETRAHPERLGL
                     EPPPAFAEPVLALLERRRAARPSFEATISTYAAALDVLTLLGGEAVAPELAREIEAWL
                     RAAFEDAADEDRFTIARSLADPELFGRALDGLGPGPLPVVLATRLREAAIMCSVRPDL
                     DEARLEELPSSDLDGSLLLAGEYVAARVAFAETFPEHAGGRLDGDGMTRALTALGRFG
                     LLAGQAFGAGELERVRAGVISTEARALIRYFDASPTSTHAILRRAQGVPGTMVEAVLR
                     ASNAARAGEAAARAEEAELRARLERRERTLRLARNVLGAVAAAAALALFGWMLADRGT
                     DLGTVATAFALAGLLFVAVALALNLAGLAPAWARKTGGAVAGGFWGITGALTGLFRED
                     DDERGS"
     gene            4582..>4873
                     /locus_tag="VFO88_00885"
     CDS             4582..>4873
                     /locus_tag="VFO88_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544108.1"
                     /translation="MAARALRIAGRLARAGRLVDGWLEIRGGRIAAVEAGRPPRGAER
                     HDGVVAPGLCDLQVNGGAGANVTDGAAALDAIDALQLDHGVTSYLPTVISTAP"
BASE COUNT          573 a         1812 c         1856 g          632 t
ORIGIN      
        1 cttctccatc ggcggcgaga tctcgtagcc ggacgtcccg ttcgggacga ccacgttcga
       61 gatctcgtcc tcgcccgtgc gcgcggtgat cgtgacgccc cacgtgatgt ccgtgccggc
      121 gttcgtgatg aagatcttcg agccgttcac gacccactgc ccgtcgcgga gctccgcccg
      181 cgtctgcgtc gcgcccgcgt cggagcccgc gcccggctcc gtgaggccga acgcgcccag
      241 cctgcgaccc gaggcgaggt cggggagcca ctcgcgcttc tgctcctccg tcccgtagag
      301 gaagaccggc atcgtcccga gcgacgtgtg cgcggcgacc gtgatcgcca cggacgagtc
      361 gacgcgcgtg agctcctcga tcgcgatcgc gtaggcgagc gtgtccgagc ccgcgccctc
      421 gtactcctcg gggatcggga tccccatgag cccgagctcg gcgagctcgg agacgatgtc
      481 gtacgggaag cggtgctcgc ggtcgagctc ctcggcgacc ggtgcgatgc gctgctccgc
      541 gaactcgcgg acggtcgtcc ggatcagctc gtgctcgtcg gagaggtcgt aggagaggct
      601 catcggtccc tggtcagggc ggtcgcgagc tcggcgatca gacgaacgcc gaagcccgtg
      661 gcccccggcc ggggatagta atcgcgcccg cgatcgaggt acgcggtacc ggcgatgtcg
      721 aggtgcgccc acggcccctc gccggcgaag cgctcgagga aggcggcgcc gatcgaggag
      781 gagccgcgtt tcttgtccgg cgtgttcttc aggtcggcga actgggagtc gaggaaccgc
      841 gcgtacgccg ggtggagcgg catcggccag acgtggtcgc cgcttcgctc cgccgcctcg
      901 aggacggccg cgagccaggc ctcgttgttc ccgaagaggc cggcgtagag atcgccgagc
      961 gcgacctgga tcgtgcccgt gagcgtcgcg aggtcgagga ggtgcgtcgc gccctgcgcg
     1021 cgcgcgtgga ggagcgcgtc ggccaggacg aggcggccct cggcgtcggt gttcgtcacc
     1081 tcgatcgtcg tcccgtcggc cgcacggacg atgtcgcccg gccggtacgc gtggccggcc
     1141 ggcatgttct cggcggccgc gacgacggcg acgatgcgga gggaaaggcc gagctcggcg
     1201 atcgccgcga gcccggccag cgtcgccgcg gctcccgcca tgtccgactt catctcctcg
     1261 agcgcgtccg ccggcttgag cgagatgccg ccggtgtcga aggtgatccc cttgccgacg
     1321 aggccgagga cgagcgtctc ctccgcgccg gcgggctcgt agcggagggt gacgaggcgc
     1381 cccgggttgt gactgccctg cgcgaccgcg gcgagcgcgc ccatgcccgc ggcggcgagc
     1441 tcgtccgggc cgatgacctc cgcgtcgatc gcgtcgaacc ggtcggcgac cgcgacggcc
     1501 gcgtcggcga gcgcggccgg ggtcagctcg ttggcgggcg cgttcacgag gtcgcgcgcg
     1561 tggttctgcc agccggcgac gacgccttcg gtccgcgcgg cctcctcggc ctccgcggcg
     1621 cgcttgccca cgaggacgag ccgttcgacc cgctcctcgt ccgcagggtc gcgcttccag
     1681 cggccggtgt cgtgcgaggc gagcgcgacg ccgtcgacca cggcgcgcgc ctcggccgcc
     1741 gtctcgagcc cggtcgtctc gccgtcgagg agccaggcaa ccgtgcggcc cggagcctcc
     1801 cccgcggcgc cggcgacacg gcaggcggcc gcgcggacgg cgtccgcgtc gaggtcgcgc
     1861 ccggcgccga ggccgacgag ggcgacgcgc cgcggcgctc cctcgccgtc ggtgtcgagg
     1921 acggtcacgg tgccgcggcg gccggtcgcg tccccgtccg agacggcgcg cccgagccgt
     1981 ccgccgagcg cctcgtcgag ctcgcgggcg gcgggggaga gcgcgggtgg ctcggcgagg
     2041 gcgaccccga ggacgtcagc ctcgacgcgg ctcgccggcg tcgagagcgc ggcggtgtcc
     2101 atgcggcgac gatgctagct acccgttcga gggatacgcg cggggcgggc gggggccgat
     2161 acgcggtgtg cgcccgaaag gcgcacgctc taccctctcc gcccgctacc tccctccctc
     2221 ctgcgggcac ctctcccgtc agaagccctc cgccccgggg ggcttctgtg ctttccggaa
     2281 ccggcaggtg gtgcccggcc gcacgacgcg caagaattgc gccgtgggag cgtcacggtg
     2341 acgagcgagg ccgagcccgg tccggccggc gtcaccgcga gcgccctcgg gctgccgcgc
     2401 tactcggtcg cgctcgtgca gagcgagctc gagatgctga acacgcccgg caacgagctc
     2461 tccgcgttcc tcctcgccga cccgggccgc tggcggacgg aggtcttcac cgagcggacg
     2521 ttcgaggcgg cgctcggctc ggcgaaccgc ttcgactgcg tcatcctcgg cttcaacgcg
     2581 gtctaccaga gcgacgcgat ccaggaggcg ctcgccgact ggacggacgt gacgggcctc
     2641 gtcgtcctcc accagctcaa gcagcgcggc ctgagcttcc tcccgcccga tctcgcgctc
     2701 acgctgaacg agctgcggga ggaggcgcgc ggcgccgtcg tccaccggca cacgcgcccc
     2761 caggacgaga tcctgctcaa ctggcccgag cccgtcgcgg agccggccgc gaagctcggg
     2821 accgtgacga gcgcggtcat gtgccacctc gagacgctcg aggccggcac gtggcggacg
     2881 tcgctcgagc tcgaggtcgg gacgcggcgg ctgcccgtgc tcctccggac gtcgagcctg
     2941 cggcggccgc gggtgatcgt ctgcacgctc cagctcgagc cgcggaagcc gctccacgcg
     3001 gcgctcctgc gcaacatgat cacgtactgc gcggcgggct ggcccgagat cgcaatcgcg
     3061 acggaggagc ggagcggcga gggcgccgac atcgcccgca agctgcgcgt gcagggcgcg
     3121 aacgccgtcg agctcgggat cgacgagccc ggccgcctgc acttcgaccg gtggcccctg
     3181 cgcggcgtcc acgaggtcgt cgtcgacgag cgctgggccg ccggcgacgt gctcggctcc
     3241 gtcgaggcgc gcgaatggct cgaggtcggg aacacgctcg tccgcgtcgg gagcgggggg
     3301 ctgacgttcc accacggcgc gtccgacgcg cacctcgtgg gggagcggtg ggccacgtgg
     3361 ttccacggga tcgaccacgc gacgtggcac ggcggcgcca cgcgcgacgg gggcgaggag
     3421 ccgggcagca tcttcgggac gcgcgcggtc ttccgcgtgc tcgagctgct cgcacgcgac
     3481 gagacgcgcg cgcaccccga gcggctcggg ctcgagccgc cgccggcgtt cgccgagccg
     3541 gtcctcgcgc tcctcgagcg gcggcgcgcg gcgaggccga gcttcgaggc gacgatcagc
     3601 acctacgcgg ccgcgctcga cgtcctgacc cttctcggcg gcgaggcggt cgcgcccgag
     3661 ctcgcccgtg agatcgaggc gtggctgcgc gccgcgttcg aggacgcggc cgacgaggac
     3721 cgcttcacga tcgcccgctc gctcgccgac ccggagctct tcggccgggc gctcgacggg
     3781 ctgggcccgg gcccgctccc cgtcgtgctc gcgacgcgcc tccgcgaggc ggccatcatg
     3841 tgcagcgtcc gccccgacct cgacgaggcc cggctcgagg agcttccctc ctccgacctc
     3901 gacgggagcc tgctgctcgc cggcgagtac gtcgccgcgc gcgtcgcgtt cgcggagacg
     3961 ttcccggagc acgcgggcgg gcggctcgac ggcgacggca tgacgcgggc gctcacggcg
     4021 ctcggccggt tcggcctcct ggccggccag gcgttcggcg ccggcgagct cgaacgcgtg
     4081 cgcgccggcg tgatcagcac ggaggcgcgg gcgctcatcc gctacttcga cgcctccccg
     4141 acctcgaccc acgcgatcct gcgccgcgcg cagggcgtgc cggggacgat ggtcgaggcg
     4201 gtcctcaggg cctcgaacgc cgcgcgggcc ggggaggcgg cggcccgggc cgaggaggcg
     4261 gagctccgcg cgcggctcga gcgccgcgag cggacgcttc ggctggcgcg gaacgtcctc
     4321 ggggccgtcg cggccgcggc cgcgctcgcg ctgttcggct ggatgctcgc cgaccgcgga
     4381 accgacctcg gcaccgtcgc gacggcgttc gccctcgccg ggctgctctt cgtcgccgtc
     4441 gcgctcgcgc tcaacctggc cgggctcgcg ccggcctggg cgcgcaagac cgggggcgcc
     4501 gtcgccggcg ggttctgggg gatcacgggt gcgctcaccg gtctcttccg cgaagacgac
     4561 gacgagcgcg gcagctgacg cgtggccgca cgcgcgcttc ggatcgcagg tcggctcgcg
     4621 cgcgcgggga ggctcgtcga cgggtggctc gagatccggg gcgggcggat cgccgcggtc
     4681 gaggccgggc ggccgccgcg cggcgccgag cggcacgacg gcgtcgtcgc cccgggcctc
     4741 tgcgacctcc aggtgaacgg cggcgccggc gcgaacgtca cggacggcgc tgccgccctc
     4801 gacgcgatcg acgcgctcca gctcgaccac ggggtgacga gctacctgcc gacggtcatc
     4861 tcgacggcgc ccg
//
LOCUS       DASTFC010000027         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1005765,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000027 DASTFC010000000
VERSION     DASTFC010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005765"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1102)
                     /locus_tag="VFO88_00890"
     CDS             complement(32..1102)
                     /locus_tag="VFO88_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9544109.1"
                     /translation="MGSEQAVASGSPLLEHDEAKALVAHGQESGSLTQGEVTILLDEL
                     GADEAQAEELYRALEELHVEVVQTPEPDVDLRYDEREMSTDALQLFLKDIGRVPLLTA
                     AQEVELAKRIERGEMPAKQKMVESNLRLVVSIAKNYRNQGLPFLDLIQEGTIGLVRAA
                     EKFDHRKGFKFSTYATWWIRQAVARALADKARTIRMPVHVVEKLNKIGRTERKLLAEL
                     GRDPSCEEIAAELDLQPQDVEHVRRSAQVPVSLEKPVGDEEESEFGHFIADESELSPD
                     EAAHTTIREEVLYRVLSGLSYRERRILEMRYGLSGEQPRTLDEVGKTFNVTRERVRQI
                     ENQSLKKLQAMAESQKLREVAA"
     gene            complement(1267..2271)
                     /locus_tag="VFO88_00895"
     CDS             complement(1267..2271)
                     /locus_tag="VFO88_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9544110.1"
                     /translation="MSARAKIVLLVGLAAVLAIGAVVAGALLLGDDTPAATEAPAPTG
                     TEPAEPGEERPPALELAVIDRDDEDATELRRGEFLYEEGDPDGARESFQAVLERDPDS
                     IEAAIGAAYASWPDGTLEELEALALRHPTSAVLRLNLGLVRLALGDLEAAQDEWREAE
                     EGEPDAPAALKAEDLLNPQSPPGRPQFFLTGFPNEAAGLTIGKRIDALTRRARAGDAR
                     DWLFLGSTLEQAGRRISAQRAYDRALELEPSSLDARVAAIVARFDKDDPSAAFSQLGP
                     LASRNRDEPVVSFHLGLMLLWLPDLDRAREQLTAARTAAPRSFYGRQADRILTELANA
                     "
     gene            2360..>3208
                     /locus_tag="VFO88_00900"
     CDS             2360..>3208
                     /locus_tag="VFO88_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET9544111.1"
                     /translation="MPSYRDLLARTKAEISEIDAPAAHERLADEETLFLDVREASEWD
                     EGHVPGAVHIPRGSLESRAEGLLPDRSRPVVVYCAGGNRSAFAAKSLEELGYEDVASL
                     TGGFADWKRNGFPFEIPQTLEAAQRRRYSRHLLIPEVGEDGQAKLLASRILLIGAGGL
                     GSPASLYLAAAGVGTLGIVDDDAVDETNLQRQIVHTTDRLGEGKAESAKRTLEALNPD
                     VTVKVFAERLTSENADRILGEGWDVIVDGADNFPTRYLLNDASVWHDIPVVHGSIFRF
                     EGQTTVF"
BASE COUNT          421 a         1173 c         1133 g          481 t
ORIGIN      
        1 aggtgacggg gctcgaggcc gcgccgcgcc atcaggcggc gacctcccgc agcttctgcg
       61 actcggccat cgcctggagc ttcttcaggc tctggttctc gatctggcgg acgcgctcgc
      121 gcgtcacgtt gaacgtcttg ccgacctcgt cgagcgtccg cggctgctcc ccggaaaggc
      181 cgtagcgcat ctcgaggatg cggcgctcgc ggtacgagag cccggagagg acgcggtaga
      241 ggacctcctc gcggatcgtc gtgtgcgcgg cctcgtcggg cgacagctcg gactcgtcgg
      301 cgatgaagtg tccgaactcc gactcctcct cgtctccgac gggcttctcg agcgacacgg
      361 gcacctgtgc gcttcgccgc acgtgctcga cgtcctgcgg ctgcaggtcg agctcggccg
      421 cgatctcttc gcaggaggga tcgcgcccga gctcggcgag cagcttccgc tccgtgcggc
      481 cgatcttgtt cagcttctcg acgacgtgga cgggcatgcg gatcgtccgc gccttgtccg
      541 cgagcgcccg cgcgaccgcc tggcggatcc accacgtcgc gtacgtcgag aacttgaagc
      601 ccttgcggtg gtcgaacttc tccgccgcgc gcacgaggcc gatcgtcccc tcctggatca
      661 ggtcgaggaa cgggaggccc tggttgcggt agttcttcgc gatcgagacg acgagccgga
      721 ggttcgactc gaccatcttc tgcttcgcgg gcatctcgcc gcgctcgatc cgcttcgcga
      781 gctcgacctc ctgcgccgcg gtgaggagcg ggacgcgccc gatgtccttc aggaagagct
      841 ggagcgcgtc cgtcgacatc tcgcgctcgt cgtaccggag gtctacgtcc ggctccggcg
      901 tctggacgac ctccacgtgc agctcctcga gcgcccggta cagctcctcc gcctgggcct
      961 cgtccgcgcc cagctcgtcc aggaggatcg tgacctcccc ctgcgtgagg gaccccgact
     1021 cctgcccgtg cgcgacgagc gccttcgcct cgtcgtgctc gagaagcggc gaccccgatg
     1081 ccactgcctg ctccgacccc atgacaccct ccttcacggt agaaccgtct cccccagcgg
     1141 cccccctcgg gccggcagta cggaaaccgt gtgcgtgggc gactctgcca gcccggggct
     1201 ccgcgtgtca tgggccgaac ggcccatttt ccgagaagcg gggtcctatt gcggaattcg
     1261 caggcgctac gcgttcgcga gctcggtcag gatccgatcg gcctgacggc cgtagaagct
     1321 tcgcggcgcg gccgttcgcg cggcggtcag ctgctcgcgc gcccggtcga gatcgggcag
     1381 ccagaggagc atgagcccga ggtggaagct gacgaccggc tcgtcccggt tccgcgaggc
     1441 gagcgggccg agctgcgaga acgcggcgga gggatcgtcc ttgtcgaagc gcgcgacgat
     1501 cgcggccacc cgggcgtcga ggctcgacgg ctcgagctcg agcgcgcggt cgtaggcgcg
     1561 ctgtgcggag atccgcctcc ccgcctgctc gagcgtgctc ccgaggaaga gccagtcgcg
     1621 ggcgtcgccg gcgcgcgccc ggcgggtgag cgcgtcgatc cgcttgccga tcgtgagccc
     1681 ggccgcctcg ttcgggaagc ccgtcaggaa gaactggggc cggccgggcg gagactgcgg
     1741 attgaggagg tcctccgcct tgagcgcggc cggcgcgtcg ggctcgccct cctccgcctc
     1801 gcgccattcg tcctgcgcgg cctcgaggtc gccgagcgcg agccgcacga gcccgaggtt
     1861 gagccggagc accgcgctcg tcgggtggcg gagggcgagc gcctcgagtt cctcgagcgt
     1921 cccgtcgggc caggacgcgt aggcggcgcc gatcgccgcc tcgatcgagt cggggtcgcg
     1981 ctcgagcacg gcctggaagc tctcgcgggc gccgtccggg tcgccctcct cgtagaggaa
     2041 ctcgccgcgg cggagctcgg tcgcgtcctc gtcgtcgcgg tcgatcacgg cgagctcgag
     2101 cgccggcggc ctctcctccc cgggctcggc gggctcggtc ccggtcggcg ccggcgcctc
     2161 cgtcgcggcg ggcgtgtcgt cgccgaggag gagcgcgccg gcgactaccg caccgatcgc
     2221 gagcacggcc gcgaggccca cgaggagcac gatcttggca cgagcggaca cctcgccgaa
     2281 tgtagcttcc aggcgaggcc gcccggcccg ccaaccgctt tcgggcggcc ggcggcgggt
     2341 cgctatactc attgaagcaa tgccgtccta ccgcgacctg ctggcccgga cgaaggccga
     2401 gatctccgag atcgacgccc ccgccgccca cgagcgcctg gccgacgagg agaccctctt
     2461 cctcgacgtg cgcgaggcgt ccgaatggga cgaggggcac gtccccggcg ccgtccacat
     2521 cccgcgcggc tcgctcgagt cgcgcgccga gggcctcctc cccgaccgct cgcggccggt
     2581 cgtcgtctac tgcgcgggcg ggaaccggag cgcgttcgcg gccaagtcgc tcgaggagct
     2641 cggctacgag gacgtcgcct cgctgacggg cggcttcgcc gactggaagc gcaacggctt
     2701 cccgttcgag atcccgcaga cgctcgaggc cgcgcagcgg cgccgctact cgcgccacct
     2761 cctcatcccg gaggtcggcg aggacgggca ggcgaagctc ctcgcgtccc ggatcctcct
     2821 gatcggcgcc ggcgggctcg gctcgcccgc gtcgctctac ctcgccgcgg ccggcgtcgg
     2881 gacgctcggg atcgtggacg acgacgccgt ggacgagacg aacctccagc gccagatcgt
     2941 ccacacgacc gaccgcctcg gcgagggcaa ggccgagtcg gcgaagcgga cgctcgaggc
     3001 cctgaacccc gacgtgacgg tgaaggtctt cgcggagcgg ctcacgtcgg agaacgccga
     3061 ccggattctc ggcgagggct gggacgtgat cgtggacggc gcggacaact tccccacgcg
     3121 ctacctcctg aacgacgcgt cggtctggca cgacatcccc gtcgtgcacg ggtcgatctt
     3181 ccgcttcgag ggccagacga cggtcttc
//
LOCUS       DASTFC010000028         5057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_165279,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000028 DASTFC010000000
VERSION     DASTFC010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165279"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFO88_00905"
     CDS             <1..1041
                     /locus_tag="VFO88_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET9544112.1"
                     /translation="GELDALTICVPTPLSKTRTPDIGYVVSAAESVAANTRPGQLVVL
                     QSTTSPGTTEEIVKPIFERAGAIVGQDVFLGYAPERVDPGNRRYTIKSTPKLVAGVTG
                     ECLRRTELLYRQIVDEVVPVSSPIVAETAKLHENTFRAVNIALANELALMCDRLGISP
                     WEVIEAAATKPFAFLPHYPGPGLGGDCIPVVPQFLAARVREYGYSPRLIDAAHEINTS
                     MPGYVVGKVADALNEDGKPVKGSRLLLLGMAYKADVHDTRESPSLEIMRLLLERGGEV
                     RYCDPWVPDLELDGVRHRSVGWTREEVEAADCVVLLTNHRQFLDEPLWDAAARIVDSR
                     NVVPDGPSVHRI"
     gene            1045..1923
                     /locus_tag="VFO88_00910"
     CDS             1045..1923
                     /locus_tag="VFO88_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9544113.1"
                     /translation="MPSLLVLGAGYVGARVAELALERGDEVVLADDWSLTRREQVAGL
                     ESRGARVVTADVASREDVERALGLGCDRLVFLAAQASRPLAEREPDRTERTNATGARL
                     VAETVAGRGGPPVVFGSSLHVYGEGLAGEVSPSHPYGEQRDLTHLTKVYAELALALHL
                     RRAGVPLALMRLGIVYGPSPVEHDGPESVTVVDKFRRLAALGEPLPVEGGGRATIGVV
                     HVEDAARILLECEPRDGVAAENVAAETITVADVAALAEGREPAGDAPFRFASPFEYRH
                     RVADYLAGGHARETRA"
     gene            1920..2798
                     /locus_tag="VFO88_00915"
     CDS             1920..2798
                     /locus_tag="VFO88_00915"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9544114.1"
                     /translation="MRLLVTGATGFLGSRLADLLAARGHDVVRLARPGGRPRAAARRE
                     DLVRVDAGDPAARELVAGCDAVLHFAGVPSPTVAAADPARAVRENAGTTANLLDACAE
                     HGAGLVYPSTTRAALDPPPDAYALSKRLGEEACRLHGARATVVRLTSVFGPGQVAAEG
                     ATGAIASFAHAALAGDPIVIPGNPDRARDFVLADDVAAPLERIVSEGRWGETIALARG
                     TSTPLREAAELVRRAAGTGVAIETPGGDLAPGENESYEAAPPPAWLGFEPIPLEEGIE
                     RYVGWLRSHPAAQGRP"
     gene            2752..4746
                     /locus_tag="VFO88_00920"
     CDS             2752..4746
                     /locus_tag="VFO88_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9544115.1"
                     /translation="MSAGSAAIPQLKAAPRAEQIAERLEGGPWRGLELALLPPDVADD
                     DAVARAIETVRRATDGHDLALTAEAPVSWPSGAFVRVDRLTDEARACIERSVRFAEAI
                     GSPVLTIHLYAPLAPDEFRAAPPLDEEAVQEFLRFFAAACEAHGVTPLVENVPPVLRM
                     RVGGVFLSQVGGHWRDLRRWHARVPELRFTFDTSHAGLFRTFAAAYPSLFGLESDEEL
                     DLARYVEELAPSLEVAHVSDAHGLLGEGLPLGDGELDLDPVTRRLGELCRFVVAEVNE
                     PDPARSPAMKDGYRRIERALAAAAAPLPRPPRRLPTGAADWSLVLGRRDPVPALLELQ
                     ERFGGRRVLVTGGAGSIGRALASFLGGLRPERITLVDTHEAALTADRRAREPVEPVAH
                     VLCDIRDGGRVERAVAEARPDVVFHLAAYKHVDWAELYPEEFVDTNLAGSWNVLRAAE
                     RAGVDTVVVASTDKAALAASVYGRTKRFMEQLTAFAARETGGRRIAVRFVNVLGTAGS
                     VSELFLRQAREGIPLTVTDAGMIRYWITMAHAATLAAHSALLAREDVALATASDAVTL
                     TVGDLAERIWRGAGRRGAPAVDVVGIRPGETMSEVLVGPGEELGTERFQGIAPIEGDV
                     PTAAPAWVLERLPERGSREDVRAVWLEALSRPGLLAPATR"
     gene            complement(4790..>5057)
                     /gene="upp"
                     /locus_tag="VFO88_00925"
     CDS             complement(4790..>5057)
                     /gene="upp"
                     /locus_tag="VFO88_00925"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET9544116.1"
                     /translation="IASRDVLLLDPMLATGHSTAYATRLVKEAGAASVRLIALIAAPE
                     GVRHMQDEHPDVSIVVAAIDRQLNELGYIMPGLGDAGDRLFGTK"
BASE COUNT          599 a         1909 c         1900 g          649 t
ORIGIN      
        1 ggcgagctcg acgcgctcac gatctgcgtc ccgacgccgc tctcgaagac gcggacgccc
       61 gacatcggct acgtcgtctc cgccgccgag tcggtcgcgg cgaacacgcg ccccgggcag
      121 ctcgtcgtcc tccagtcgac gacctcgccg gggacgacgg aggagatcgt gaagccgatc
      181 ttcgagcggg cgggcgcgat cgtcgggcag gacgttttcc tcggctacgc gcccgagcgc
      241 gtcgacccgg gcaaccggcg ctacacgatc aagtcgacgc cgaagctcgt cgcgggcgtg
      301 accggcgagt gcctgcggcg caccgagctc ctctaccggc agatcgtgga cgaggtcgtg
      361 cccgtctcga gcccgatcgt cgccgagacg gcgaagctgc acgagaacac gttccgcgcc
      421 gtgaacatcg cgctcgcgaa cgagctcgcg ctcatgtgcg accggctcgg gatctccccc
      481 tgggaggtga tcgaggccgc ggcgacgaag ccgttcgcgt tcctcccgca ctatccgggg
      541 ccgggcctcg gcggggactg catcccggtc gtgccgcagt tcctcgcggc gcgcgtgcgc
      601 gagtacggct actcgccgcg gctgatcgac gcggcccacg agatcaacac gagcatgccg
      661 ggctacgtcg tcgggaaggt cgcggacgcg ctgaacgagg acggcaagcc ggtgaagggc
      721 tcgcgcctcc tcctgctcgg gatggcctac aaggcggacg tgcacgacac gcgggagtcg
      781 ccgtcgctcg agatcatgcg gctgctgctc gagcgcggcg gcgaggtgcg ctactgcgac
      841 ccgtgggtgc cggacctcga gctggacggc gtccgccacc ggagcgtcgg gtggacgcgc
      901 gaggaggtcg aggcggccga ttgcgtcgtc ctgctcacga accaccgcca gttcctcgac
      961 gagccgctct gggacgcggc cgcgcggatc gtggactcgc ggaacgtcgt gcccgacggg
     1021 ccgtcggtgc accgcatcta gcgcgtgccc tcgctgctcg tcctcggggc ggggtacgtc
     1081 ggcgcgcggg tggcggagct ggcgctcgag cgcggcgacg aggtcgtgct cgcggacgac
     1141 tggtcgctca cccggcgcga gcaggtggcg gggctcgagt cgcgcggcgc gcgggtcgtg
     1201 acggccgacg tcgcgtcgcg cgaggacgtc gagcgggcgc tcgggctcgg ctgcgaccgg
     1261 ctcgtcttcc tcgccgcgca ggcgagccgg cccctcgccg agcgcgagcc cgaccggacc
     1321 gagcggacga acgcgacggg ggcgcgactc gtcgccgaga cggtcgcggg gcgcggcggg
     1381 ccgccggtcg tgttcggctc gtcgctccac gtgtacgggg aggggctcgc cggcgaggtg
     1441 tccccgtcgc acccgtacgg cgagcagcgc gatctcaccc atctgacgaa ggtctacgcg
     1501 gagctcgccc tggcgctcca cctgcgccgg gccggggtgc cgctcgcgct catgcggctc
     1561 ggaatcgtct acgggccgag cccggtcgag cacgacgggc cggagtcggt gacggtcgtg
     1621 gacaagttcc ggcggctggc ggccctcggc gagccgctcc cggtggaggg cggcggccgt
     1681 gcgacgatcg gcgtcgtgca cgtcgaggac gcggcgcgga tcctcctcga gtgcgagccg
     1741 cgcgacgggg tcgccgccga gaacgtcgcc gcggagacga tcacggtcgc ggacgtcgcc
     1801 gcgctcgccg agggccgcga gcccgccggg gacgcgccct tccgcttcgc gagccccttc
     1861 gagtaccggc accgcgtggc cgactacctg gccggcgggc acgcacgaga gacacgagcg
     1921 tgaggctgct cgtcacgggg gcgacggggt tcctcggctc gcggctcgcc gacctgctcg
     1981 ccgcccgggg ccacgacgtc gtccggctcg cgcggccggg tgggcgcccg cgcgcggcgg
     2041 cgcggcgcga ggatctcgtc cgcgtcgacg cgggcgaccc ggcggcgcgg gagctcgtcg
     2101 ccggctgcga cgcggtgctc cacttcgcgg gcgtgccgag cccgaccgtc gccgcggccg
     2161 accccgcccg cgccgtgcgc gagaacgcgg gcacgacggc gaacctcctc gacgcgtgtg
     2221 ccgagcacgg tgcgggcctc gtctacccgt cgaccacccg ggccgcgctc gacccgccgc
     2281 ccgacgccta cgccctctcg aagcggctcg gcgaggaggc gtgccgcctg cacggcgccc
     2341 gcgcgaccgt agtccgcctg acctccgtct tcggccccgg ccaggtcgcg gccgaggggg
     2401 cgaccggcgc gatcgcgagc ttcgcgcacg cggccctcgc gggcgacccg atcgtcatcc
     2461 cggggaaccc cgaccgcgcc cgcgacttcg tcctcgccga cgacgttgcg gcgccgctcg
     2521 agcgcatcgt ttcggagggc cgctggggcg agacgatcgc gctcgcgcga ggcacgtcga
     2581 cgccgcttcg cgaggccgcc gagctcgtcc ggcgcgccgc cggaacgggc gtcgcgatcg
     2641 aaacccccgg cggcgatctc gcgcccggcg agaacgagag ctacgaggcc gccccgccgc
     2701 ccgcctggct agggttcgag ccgatcccgc tggaggaggg aatcgagcgc tatgtcggct
     2761 ggctccgcag ccatcccgca gctcaaggcc gcccctaggg ccgagcagat cgccgagcgc
     2821 ctcgaaggag gcccgtggcg cgggctcgag ctggcgctcc tgccgcccga cgtcgccgac
     2881 gacgacgcgg ttgcgcgcgc gatcgagacc gtgcgccgag cgaccgacgg gcacgacctt
     2941 gccctcaccg ccgaggcgcc cgtctcgtgg ccgagcggcg cgttcgtccg cgtcgaccgg
     3001 ctgacggacg aggcgcgtgc gtgcatcgag cgcagcgtcc ggttcgcgga ggcgatcggc
     3061 tcgccggtgc tcacgatcca tctttacgcg ccgctcgctc cggacgagtt ccgcgcggca
     3121 ccgccgctcg acgaggaggc cgtgcaggag ttcctccgct tcttcgccgc cgcgtgcgag
     3181 gcgcacgggg tgacgccgct cgtcgagaac gtcccgcccg tactgcggat gcgcgtcggc
     3241 ggcgtcttcc tctcgcaggt cggcggccac tggcgcgacc tccgccgctg gcacgcgcgc
     3301 gtcccggagc tccgcttcac cttcgacacg tcgcacgcgg ggctcttccg gacgttcgcg
     3361 gccgcgtacc cgtcgctctt cgggctcgag tcggacgagg agctcgacct cgcccgctac
     3421 gtcgaggagc tcgcgccgtc gctcgaggtc gcgcacgtct cggacgccca cggcctgctc
     3481 ggcgagggcc tgccgctcgg ggacggcgag ctcgacctcg accccgtgac gcgccggctc
     3541 ggcgagctct gccgcttcgt cgtcgcggag gtgaacgagc ccgacccggc ccgctcgccg
     3601 gcgatgaagg acggctaccg gcggatcgag cgggcgctcg ccgccgccgc cgcgccgctc
     3661 ccccgcccgc cgcgccgcct tccgaccggc gccgccgact ggtcgctcgt cctcgggcgg
     3721 cgcgacccgg tgccggccct gctcgagctg caggagcgct tcggcgggcg ccgcgtgctc
     3781 gtcaccggcg gcgcggggtc gatcggccgc gcgctcgcgt ccttcctggg cggccttcgg
     3841 ccggagcgga tcacgctcgt cgacacccac gaggcggcgc tgacggccga ccgccgcgcc
     3901 cgcgagccgg tcgagcccgt cgcccacgtc ctctgcgaca tccgcgacgg cggccgcgtg
     3961 gagcgtgccg tggccgaggc ccgcccggac gtcgtcttcc acctcgcggc ctacaagcac
     4021 gtggactggg ccgagctcta ccccgaggag ttcgtcgaca cgaacctcgc cgggagctgg
     4081 aacgtcctcc gcgccgccga gcgcgcgggc gtcgacacgg tggtcgtggc ctcgacggac
     4141 aaggccgcgc ttgccgcgag cgtctacggg cgcacgaagc gcttcatgga gcagctcacc
     4201 gcgttcgccg cgcgcgagac gggcggccgg cggatcgccg tgcgcttcgt gaacgtgctc
     4261 ggcacggcgg gcagcgtgtc ggagctcttc ctccgccagg cccgcgaggg catcccgctc
     4321 acggtgacgg acgccgggat gatccgctac tggatcacga tggcgcacgc ggccacgctt
     4381 gccgcccact ccgcgctcct ggcccgcgag gacgtggcgc tcgcgaccgc ctcggacgcc
     4441 gtcacgctca cggtcggcga cctcgccgag cggatctggc gcggagccgg ccggcgaggc
     4501 gcgccggcgg tcgacgtggt ggggatccgg ccgggcgaga cgatgagcga ggtgctcgtc
     4561 ggccccggcg aggagctcgg gacggagcgc ttccagggca tcgcgccgat cgagggagac
     4621 gtcccgacgg cggcccccgc ctgggtgctc gagcgcctcc ccgagcgggg gagccgcgag
     4681 gacgtcaggg ccgtgtggct cgaggcgctc tcccggcccg gcctcctcgc gcccgcgacc
     4741 cggtagcaag taacactgtg ttacttgcgg tgggcgaccc ggcgcgcctc tacttcgtgc
     4801 cgaacagccg gtcgccggcg tcgccgagcc ccggcatgat gtagccgagc tcgttcagct
     4861 ggcggtcgat cgcggcgacg acgatcgaga cgtccgggtg ctcgtcctgc atgtggcgga
     4921 cgccctccgg cgccgcgatg agcgcgatca gccgcacgct cgccgcgccc gcctccttca
     4981 cgagccgcgt cgcgtacgcc gtcgagtggc ccgtcgcgag catcgggtcg aggaggagca
     5041 cgtcgcgcga cgcgatg
//
LOCUS       DASTFC010000029         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_560778,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000029 DASTFC010000000
VERSION     DASTFC010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560778"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..916)
                     /locus_tag="VFO88_00930"
     CDS             complement(170..916)
                     /locus_tag="VFO88_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9544117.1"
                     /translation="MSDHGSRLPGADTLVFVPAWNEEENLPAVLDDLRAVLPDADVLV
                     VDDGSTDGTAAVAREGGAKVLSFSANRGLPAAIGAGYAYAEQNGYAFCGRVDADGQHP
                     AAELARLLALVRGGSCDVAVGSRFVSGEGYEPYRYRPSPARRLGTAVLRRAMALVLRR
                     PFGDATSGLWAVNATALPVLARPFTTAAPEVEALIRLADAGLRVEEVPVNMAARAGGV
                     SKLRGGKAVRVVLTVTGALVLAKVLSARRR"
     gene            complement(958..1500)
                     /locus_tag="VFO88_00935"
     CDS             complement(958..1500)
                     /locus_tag="VFO88_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544118.1"
                     /translation="MQGQKRWRIIALLATGIAIGVVMAATPATGHISGWAHNWTQHIK
                     PKTDARYYTKQQSNDRFRNHIPGGPLAGASVGADGTLIKWFNRRGGAPTIEKLGTGAY
                     RLTFPGLEGQAAVNNSVALVSLLDSGQIYRTSSSGNPYIFTSDSAGAAADRSFDLVIF
                     RPGALLRPAVARGGPGGTDG"
     gene            1719..>2159
                     /locus_tag="VFO88_00940"
     CDS             1719..>2159
                     /locus_tag="VFO88_00940"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HET9544119.1"
                     /translation="MRIEEPQELFEHKLGAALKMESTAAEMLATLTEKTQSPELRGIL
                     LRHADETQHQIVNLHEVFAAIGADPKEKASAAIRGIETEGQTLIRMTDESLVDAVVLA
                     SAAETEHHEIAVYESLIAYAEALGHGTVVGLLRENLRQEERMLEE"
BASE COUNT          327 a          767 c          778 g          287 t
ORIGIN      
        1 catgtgcggg tgccggggaa gctgctgctg cgcgggcgca gcagcggcga gcgctccgac
       61 catgaccgcc gcagttgcga gaacgagaag aactcgcgtc atgatcaccc tcctcgggtt
      121 gatgtcgggg cgcggaacgt atacggcgcg gcccgcgtgc gcaaccggct cagcgacggc
      181 gcgcgctcag caccttggcg aggacgagcg cgcccgtgac cgtcaggacg acgcggaccg
      241 ccttgccgcc gcggagcttc gagacgccgc cggccctcgc ggccatgttc acgggcacct
      301 cctcgacgcg caggcccgcg tccgcgaggc ggatcagcgc ctcgacctcc ggcgccgccg
      361 tcgtgaacgg gcgcgcgagc accgggaggg cggtcgcgtt caccgcccag agcccgctcg
      421 tcgcatcccc gaacgggcgg cgcaggacga gcgccatcgc gcgccggagc accgccgtcc
      481 cgagccggcg ggccgggctc ggccggtagc ggtacggctc gtagccctcg cccgagacga
      541 accgcgagcc gaccgcgacg tcgcaggagc cgccccgcac gagcgcgagc agcctcgcga
      601 gctcggcggc cggatgctgg ccgtccgcgt cgacccgccc gcagaacgca tagccgttct
      661 gctcggcgta cgcgtagccg gcgccgatcg cggcggggag cccgcggttg gcggagaagg
      721 agagcacctt cgcgccgccc tcccgcgcga ccgcggccgt cccgtccgtc gagccgtcgt
      781 cgacgacgag cacgtccgcg tccgggagca ccgcccgcag gtcgtcgagc accgcgggga
      841 ggttctcctc ctcgttccag gccgggacga agacgagcgt gtccgccccg ggaaggcggg
      901 agccatgatc gctcacggct cccgcctctg cccggacacg cggatcacgc ggatcgtcta
      961 gccgtccgtg ccgcccgggc cgccgcgcgc gacagccggt cggagcagcg ctccgggccg
     1021 gaagatgacg aggtcgaagc tccggtccgc cgcggccccg gcactgtccg acgtgaagat
     1081 gtagggattc ccgctggagc tcgtcctgta gatctgcccc gaatcgagca gcgagacgag
     1141 tgcgaccgag ttgttgaccg cggcctgtcc ctcgagcccg gggaacgtca ggcgatacgc
     1201 accggttccc agcttctcga tggtcggggc gccgccgcgc cggttgaacc acttgatgag
     1261 cgtcccgtcc gcgccgacgc tcgcccctgc gagcggtccg ccggggatgt gattccggaa
     1321 ccggtcgttc gactgctgct tcgtgtagta gcgcgcgtcc gtcttgggct tgatgtgctg
     1381 cgtccagttg tgtgcccagc ccgagatgtg gccggtcgcc ggcgtggccg ccatgaccac
     1441 gccgatcgcg atgcccgtgg cgagcagcgc gatgatgcgc cacctcttct gtccctgcac
     1501 ctgcgctccc ttcgttgacg gttcgccggc acaagccggc gcggctctcc tgggtgggac
     1561 gtcgtgtggg tcgtgccacc cggagcgcca agtaaaggga tcgcgtcggc tggaatcaat
     1621 ccccaggtcg ggggatcggt taccggcgct aggcgggggg tacggcgcca gtgaccagga
     1681 ccgaccgcga agatcggggg cagttgatgc tcgaagcgat gaggatcgag gagccgcagg
     1741 agctcttcga gcacaagctc ggcgcggcgc tcaagatgga gtcgacggcc gccgagatgc
     1801 tcgccacgct gacggagaag acgcagagcc cggagctccg cgggatcctc ctccgccacg
     1861 ccgacgagac ccagcaccag atcgtcaacc tccacgaggt gttcgccgcg atcggcgccg
     1921 acccgaagga gaaggcgtcc gccgcgatca gggggatcga aaccgagggc cagacgctca
     1981 tccgaatgac ggacgagtcg ctcgtcgacg ccgtcgtcct tgcgagcgcc gccgagaccg
     2041 agcaccatga gatcgccgtc tacgagagcc tcatcgcgta cgcggaggcg ctcgggcacg
     2101 gaacggtcgt cgggctcctg cgcgagaacc tccggcagga ggagcggatg ctcgaggag
//
LOCUS       DASTFC010000030         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_214722,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000030 DASTFC010000000
VERSION     DASTFC010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214722"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VFO88_00945"
     CDS             complement(<1..1274)
                     /locus_tag="VFO88_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9544120.1"
                     /translation="MFERFTERARQVVVLAQDEARALKHNYIGTEHILLGLLREEEGL
                     AARVLESLDITVEEVRAQVARIVGQGDEVTTGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADAEKIRNEIIRMLSGPGRRSSGGAASSEKSK
                     SSKLLDQFGRNLTKLAAEGKLDPVVGRQNEIERVMQILSRRTKNNPVLIGEPGVGKTA
                     VVEGLASRIATGQVPELLKNKQIYTLDLAALVAGSKYRGEFEERLKKVMKEITQRGDI
                     VLFIDELHNLVGAGAAEGAIDAASILKPALARGELQTIGATTLDEYRKYLERDAALER
                     RFQQIKVDEPSVDDSVQILRGLRDRYEAHHRCTITDDALHAAAVLADRYIQDRHLPDK
                     AIDLIDEAASRMRIKTMTAPPRYRELEEEIEKV"
     gene            1536..>2963
                     /locus_tag="VFO88_00950"
     CDS             1536..>2963
                     /locus_tag="VFO88_00950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9544121.1"
                     /translation="MRNVTGSTSADEPTSDYTVGGGATAQAVAETLAGRSRRVRVRTE
                     WALVRFALDATMLGLGVAVAESSWRGAGFSATGSIWLVALPLAALALLYVRGAYVSGL
                     QPSFFDEVRRVVGATAIAAMFVTTARVVTSNEVVPSTQAVRPWLFATACVLAGRGVLA
                     WADARARRRGETARPTLILGAGRVGRLLAQRLLERPELGLRPVGFLDKEPREGLESVP
                     VLGASWDLDRIIAEHGVENVIVTFSTAPHEVLLRIARRCEELGVEVSLVPRLFERLPE
                     RLTVEHIGGLPLITPHPADPRGWQFAAKYAFDRVAAAGLLLLASPILAVTALAVWATA
                     GRPIFFRQARIGLDGREFGMLKFRTMRGAPEENGEADAEWAARELGGEAAPAAAPAED
                     RQTGLGRYLRRASVDELPQLWNVLRGDMSLVGPRPERAHYARRFEGRIYRYAERHRVK
                     SGITGWAQVNGLRGKTSLADRVEWDN"
BASE COUNT          371 a         1037 c         1077 g          478 t
ORIGIN      
        1 accttctcga tctcctcctc gagctcgcgg tagcgcggcg gagcggtcat cgtcttgatc
       61 cgcatccgcg acgcggcctc gtcgatcagg tcgatcgcct tgtccggcag gtggcggtcc
      121 tggatgtagc ggtcggcgag cacggcggct gcgtggagcg cgtcgtccgt gatcgtgcag
      181 cggtgatgcg cctcgtagcg gtcgcgcagg ccgcggagga tctgcacgga gtcgtccacg
      241 gacggctcgt ccaccttgat ctgctggaag cgccgctcga gggcagcgtc gcgctcgagg
      301 tacttgcggt actcgtcgag cgtcgtcgcg ccgatcgtct ggagctcccc gcgcgcgagc
      361 gccggcttca ggatggaggc cgcgtcgatc gcgccctcgg ccgcgcccgc gccgacgagg
      421 ttgtggagct cgtcgatgaa gaggacgatg tcgccccgct gcgtgatctc cttcatgacc
      481 ttcttcagcc gctcctcgaa ctcgccgcgg tacttcgagc cggcgacgag ggcggccagg
      541 tcgagcgtgt agatctgctt gttcttcagg agctcgggca cctggcccgt cgcgatgcgc
      601 gaggcgaggc cctcgacgac ggcggtcttg ccgacgccgg gctcgccgat cagcaccggg
      661 ttgttcttcg tgcggcgcga gaggatctgc atgacgcgct cgatctcgtt ctggcgcccg
      721 accaccgggt cgagcttccc ctcggcggcg agcttcgtca ggttgcggcc gaactggtcg
      781 aggagcttcg agctcttcga cttctccgac gacgcggccc cgcccgacga gcggcggccc
      841 gggccggaga gcatcctgat gatctcatta cgaatcttct cggcatccgc gtcgaagtcg
      901 aggaggatgc gcgcggcgac cccctcgttc tcacggacga ggccgagcag gatgtgctcg
      961 gtgccgatgt agttgtggcc gagggacagc gcctcgcgca gcgccagctc gagcaccttc
     1021 ttcgcgcgcg gcgtgaacgg gatctggccc gtcgtcacct cgtcgccctg gccgacgatc
     1081 cgggcgacct gcgcgcggac ctcctcgacc gtgatgtcca ggctctcgag cacgcgcgcg
     1141 gccaggcctt cctcctcgcg gaggagcccg agcaggatgt gctcggttcc gatgtagttg
     1201 tgcttcaggg cgcgcgcctc gtcctgggcg agaacgacga cctgtcttgc gcgttcagtg
     1261 aagcgttcga acaagcgctc aacgtccttt cggtcgtacg ggaagaccca tggtaccgga
     1321 cgggttcggc gtcaccgagg tccgcatgcg tcctgtatcg gctccgtttc tcctttgcga
     1381 cacccccagc ttctgcgccg ggccccacgg cggaaaacca ggatgtgtca cgcaaccgta
     1441 actcgggaac gggccggtac gttgtagcgg acgaacccct tcgcccggag cgctgcggcg
     1501 ttgctcctgg ggaaggctgc ggaggaggaa ctgcggtgag gaacgtaacg gggtcgacgt
     1561 cggccgacga gccgacgtcg gactacacgg tcggcggagg ggcgacggct caggccgtcg
     1621 cggaaacgct cgcggggcgc tcgcggcgcg tccgcgtgcg gaccgagtgg gcgctcgtgc
     1681 gcttcgcgct cgacgcgacg atgctcggcc tgggcgtcgc cgtcgccgag tcgagctggc
     1741 gcggcgccgg gttctccgcc accggctcga tctggctcgt cgccctcccg ctcgccgcgc
     1801 tcgcgcttct gtacgtgcgg ggcgcgtacg tctcgggcct ccagccgagc ttcttcgacg
     1861 aggtacgacg ggttgtcggc gcgacggcga tcgccgccat gttcgtcacg accgcgcggg
     1921 tcgtgacgag caacgaggtc gtcccgtcga cgcaggccgt ccgcccctgg ctcttcgcga
     1981 ccgcgtgtgt cctcgccggg cgcggagtgc tcgcctgggc ggacgcacgg gcgcgccggc
     2041 gaggggagac ggcgcgcccg accttgatcc tgggcgcggg tcgcgtcggg cggctgctgg
     2101 cgcagcggct gctcgagcgg cccgagctcg ggctgcgccc ggtcggcttc ctggacaagg
     2161 agccgcgcga gggcctcgag agcgtcccgg tgctcggcgc gagctgggac ctcgaccgga
     2221 tcatcgccga gcacggggtc gagaacgtga tcgtcacctt ctccacggcg ccgcacgagg
     2281 tgctgctcag gatcgcgcgc cggtgcgagg agctcggcgt cgaggtctcg ctcgtcccgc
     2341 gcctcttcga gcgcctgccc gagcggctga ccgtcgagca catcggcggg ctcccgctca
     2401 tcaccccgca tccggccgac ccccgcggct ggcagttcgc ggcgaagtac gcgttcgacc
     2461 gcgtcgccgc cgccggcctc ctgctcctgg cctcgccgat cctcgccgtg accgcgctcg
     2521 cggtctgggc gaccgccggg cggccgatct tcttccgcca ggcgcggatc gggctcgacg
     2581 ggcgcgagtt cgggatgctg aagttccgca cgatgcgcgg cgcgcccgag gagaacggcg
     2641 aggccgacgc ggagtgggcg gcgcgcgagc tcggcggcga ggccgcgccg gctgccgcgc
     2701 cggccgagga ccggcagacg ggcctcggcc gctacctgcg ccgcgcgtcg gtcgacgagc
     2761 tcccgcagct ctggaacgtc ctgcgcggcg acatgtcgct cgtcggcccg cgtcccgagc
     2821 gcgcgcacta cgcgaggcgg ttcgagggca ggatctaccg ttacgctgag cgccaccgcg
     2881 tgaagtccgg gatcaccggc tgggcgcagg tcaacgggct gcgcggcaag acctcgctcg
     2941 ccgaccgcgt cgaatgggac aac
//
LOCUS       DASTFC010000031         6062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_643132,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000031 DASTFC010000000
VERSION     DASTFC010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643132"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFO88_00955"
     CDS             complement(<1..356)
                     /locus_tag="VFO88_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544122.1"
                     /translation="MAGSDDAPILPFASRAEWEAWLEEHHGSEDAVWVKFAKKASGLP
                     TVTYDEAVEVALCFGWIDGLVRRLDDDFYLQRFTPRRKRSKWSQVNRDKALALIERGE
                     MRPAGLAEVERAQGDGR"
     gene            384..782
                     /locus_tag="VFO88_00960"
     CDS             384..782
                     /locus_tag="VFO88_00960"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9544123.1"
                     /translation="MGQHRVQELRLVVTAEDYDEALRFYRDVLGMPEVEAISDDEGRV
                     AILEAGRATLELTDPPHAAWIDELEVGRRVAGQVRVALRVDDSTAETRRLEEAGATVL
                     AEPRPTPWGSLNSRLEGPAGLQLTLFSGGE"
     gene            838..1242
                     /locus_tag="VFO88_00965"
     CDS             838..1242
                     /locus_tag="VFO88_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HET9544124.1"
                     /translation="MTHAQAMLETHPLGADLDNAKLLGCIEACYDCAQSCTACADACL
                     GEENVAMMRRCIRLCQDCADVCVATGRILSRQTEYDAGMTRAALDACAQACRICAEEC
                     ERHADHHDHCRVCAEACRHCERMCAELLVAIR"
     gene            complement(1239..2111)
                     /locus_tag="VFO88_00970"
     CDS             complement(1239..2111)
                     /locus_tag="VFO88_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9544125.1"
                     /translation="MELDRDRAVLAAFAGVSVLGGGNAVGIRFSNRELDPLWGAGLRF
                     ALAALVLLAVVAVLRLALPRGRALAGAVLFGALNFGAAFALAYYALVRLHAGFGQIVL
                     ALVPLLTLLLAVAWRQERFRLAAVAGALLALAGVAVMSQGPLREDVPALSLLAAAGSA
                     LCVAQAGVLVRRLPPVHPVTMNAVGMSAGAALLVLGALAAREPLALPDRGATWLAIAY
                     LAVVGSGVVFVLYLFVLGRWQASRAAYTFVVIPVVTVALSAWLDDEPVGLSLVFGGVL
                     VLAGVYVGALRAAR"
     gene            complement(2172..3617)
                     /locus_tag="VFO88_00975"
     CDS             complement(2172..3617)
                     /locus_tag="VFO88_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544126.1"
                     /translation="MQDTALDPRRWKALAVLGIAYLMVVLDVSIVNVALPSIQTDLNF
                     TPENLQWVVSGYALTFGGFLLLGGRMGDLLGRRTLFMVGLALFFVTSLWAGLATSDTM
                     LIIARLAQGAAGAILAPSVFSITSVTFAEGPERNKALGILGGIAGAGAAIGVLLGGVL
                     TSYISWEWIFFVNLPIAAGALVLVPRYVRESRADGMARHFDAWGAVTVTGSLMFLVYG
                     LTQSTTNGWTSWQTIGSLVAWAVLSVVFLAIEQRSHSPLVRLGIFRRRTLTGANVIGF
                     LLGTMIFGMFFLLSLYMQQVLGFSAMETGVGYLAVALTAVVSAGISQGFVTKLGVKPV
                     LTFGMGLLLAGLLYFTQVSVNGTYAGDLLPGFLLIGVGMGFSFVPISIAALAGVDGPE
                     AGLASGLINTSQQIGGALGLAILATVATTRTDNQLAAGTAPPEALTNGFTLAFWVAAG
                     FAVVSLVTTLVVLRRRDLAAAPAGEPVPVGA"
     gene            3751..4140
                     /locus_tag="VFO88_00980"
     CDS             3751..4140
                     /locus_tag="VFO88_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9544127.1"
                     /translation="METVERIMSRDLLTAAPDERLQDAAKRMVGRRVGAILVLEGERL
                     AGILTERDVLRAVGNGLDPDALVSQWMTRGPETVEPSATAEEALALMHQGGFRHLPVT
                     DGDRLVGIVSVRDVLRVGVQDASPRGV"
     gene            complement(4137..4595)
                     /locus_tag="VFO88_00985"
     CDS             complement(4137..4595)
                     /locus_tag="VFO88_00985"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HET9544128.1"
                     /translation="MSLYELLLFAHVIAAAAWFGAALLSLVLVELAVRGGDTPGVLKL
                     GEYEDRVAQVLFIPSALVVLGAGIWLTVEGPWSLGRDGWVVAGIVLLAAVFVLGIALI
                     VPAGNRLKALAVANAPDEELQDQISRIRALSWIDMALLTAAFFVMTVKPF"
     gene            complement(4709..5824)
                     /locus_tag="VFO88_00990"
     CDS             complement(4709..5824)
                     /locus_tag="VFO88_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544129.1"
                     /translation="MMRLATFLAALAAFITVAAVLTGVAAAYPAENDSFAAATVLEGP
                     NGWVGGANFKATKEPGEPAHAGNSGGASLWYRWVAPHDETMSFTAWEWGFDPVLAVYT
                     GTTLGDLVEVASNDDWDGFTSRVVFDVVAGTELWIAVDGYDGATGSFGLQWFVNPEND
                     DFADAALLEGARGDVFGSNIGATVEPGEPLVAGGTASVWYSWTAPVTGHVRFNTHGSE
                     FDTVLGVYTGTAVDALTCLCSNDDFGWYSSEVSFLAEAGTTYAIAVGGWDGETGSLEL
                     HWYPGAILQGTGAGDVLRGTPGADLILGLGGGDTLYGNGGADEIVGGAGRDRLYGGPG
                     PDFLDSRDGRRGNDAVYGGRGHDTARADRGDAIRGVP"
BASE COUNT          980 a         2171 c         2207 g          704 t
ORIGIN      
        1 cgcccgtcgc cctgcgcgcg ctcgacctcg gccagcccgg cgggccgcat ctcgccgcgc
       61 tcgatgaggg cgagcgcctt gtcgcggttg acctgcgacc acttgctccg cttgcgcctg
      121 ggcgtgaacc gctggaggta gaagtcgtcg tcgagccggc gcacgagccc gtcgatccag
      181 ccgaagcaga gcgcaacctc gaccgcttcg tcgtacgtga cggtcgggag cccggacgcc
      241 ttcttcgcga acttcaccca gacggcgtcc tcgctcccgt ggtgctcctc gagccacgcc
      301 tcccactcgg cgcgcgaggc aaacggcaga atcggggcgt cgtcgctacc agccatcgcc
      361 gcatgatcgc ggaaggaggc cgaatgggac agcaccgcgt gcaggagctc cggctcgtcg
      421 tgaccgccga ggactacgac gaggccctcc gcttctaccg ggacgtgctc gggatgcccg
      481 aggtcgaggc gatctcggac gacgagggcc gcgtggcgat cctcgaggcg ggccgggcga
      541 cgctcgagct gaccgacccg ccgcacgccg cgtggatcga cgagctcgag gtcggccgcc
      601 gcgtcgccgg gcaggtccgg gtcgcgctcc gcgtggacga ctcgaccgcc gagacccgcc
      661 gtctggagga ggccggcgcg accgtgctcg ccgagccgag accgacaccg tggggctcgt
      721 tgaactccag gctcgaaggc ccggcgggcc ttcagctcac gctcttcagc ggaggggagt
      781 agcaaccgcc gctacgggaa cgcgcgaagg gacgcacgcc gacgaaggga gaccaggatg
      841 acccacgcac aagcgatgct cgagacccat ccgctgggag ccgacctcga caacgcgaag
      901 ctcctcggct gcatcgaggc ctgctacgac tgcgcgcaga gctgcacggc ctgcgccgac
      961 gcctgcctcg gcgaggagaa cgtcgcgatg atgcgccgct gcatccggct ctgccaggac
     1021 tgcgccgacg tatgcgtcgc gaccggccgg atcctgagcc ggcagacgga gtacgacgcc
     1081 gggatgacgc gcgccgcgct cgatgcgtgc gcgcaggcgt gccggatctg cgccgaggag
     1141 tgtgagcgcc acgccgacca ccacgaccac tgccgcgtgt gcgccgaggc gtgccgccac
     1201 tgcgagcgca tgtgcgcgga gctgctcgtc gcgatccgct agcgcgccgc gcggagcgcg
     1261 ccgacgtaca cgcccgcgag gacgagcacg ccgccgaaga cgagcgatag gccgaccggc
     1321 tcgtcgtcga gccaggcgga cagcgcgacc gtcacgaccg ggatcacgac gaacgtgtac
     1381 gcggcgcgcg acgcttgcca gcgtccgagc acgaagaggt agaggacgaa gacgacgccc
     1441 gatccgacga ccgcgaggta ggcgatcgcg agccatgtgg cgccgcggtc cggcagggcc
     1501 agcggctccc gggcggcgag cgcgccgagc accagaagcg ccgcgccggc gctcatcccg
     1561 accgcgttca tcgtgaccgg gtgcaccggc gggagccggc ggacgagcac gcccgcctgg
     1621 gccacgcaga gggcgctccc ggccgcggcg aggagcgaga gcgccgggac gtcctcccgc
     1681 agcggcccct gcgacatcac cgcgacgccg gcgagcgcga gcagcgcacc ggcgacggcg
     1741 gcgaggcgga agcgctcctg ccgccacgcg accgcgagaa ggagcgtgag gagcgggacg
     1801 agcgcgagga cgatctgacc gaacccggcg tgcaggcgta cgagcgcgta gtacgcgagc
     1861 gcgaacgcgg cgccgaagtt gagcgcgccg aagagcaccg caccggcgag cgcccggccg
     1921 cgcggaagcg ccaggcggag caccgccaca acggcgagga gcacgagcgc cgcgagcgcg
     1981 aagcgcaggc ccgctcccca gagcgggtcg agctcgcggt tcgagaaccg gatcccgacc
     2041 gcgttgccgc cgccgaggac cgaaacgccg gcgaacgccg cgagcaccgc ccggtcgcgg
     2101 tcgagctcca cgccgagacg atacgagggg cggccgggcc cgcgcccggc cgcccctcgg
     2161 gttgtggtcc gctaggcgcc gaccggaacc ggctcgcccg ccggagcggc ggcgaggtcg
     2221 cggcgccgca gcaccacgag cgtcgtcacg agcgagacga ccgcgaaccc ggccgcgacc
     2281 cagaacgcaa gcgtgaagcc gttcgtcagg gcctcgggcg gagcggtgcc ggccgcgagc
     2341 tggttgtccg tccgcgtcgt cgccacggtg gcgaggatcg cgagaccgag ggcgccgccg
     2401 atctgctggc tcgtgttgat gagcccggac gcgagccccg cctccggccc gtcgacgccg
     2461 gcgagcgccg cgatcgagat cggcacgaag gagaagccca tcccgacgcc gatcagcagg
     2521 aacccgggca ggaggtcgcc cgcgtacgtg ccgttcaccg acacctgcgt gaagtacagg
     2581 aggccggcga gcaggaggcc catcccgaag gtgagcaccg gcttcacgcc gagcttcgtg
     2641 acgaagccct gcgagatccc ggccgagacg accgcggtga gcgcgacggc gaggtagccg
     2701 acgcccgtct ccatcgcgga gaagccgagc acctgctgca tgtagagcga gagcaggaag
     2761 aacatcccga agatcatcgt cccgagcagg aacccgatca cgttcgcgcc cgtgagcgtc
     2821 cgccggcgga agatcccgag ccggacgagc ggcgagtgcg agcgctgctc gatcgcgagg
     2881 aacacgaccg agaggaccgc ccacgcgacg agcgagccga tcgtctgcca ggacgtccag
     2941 ccgttcgtcg tcgactgcgt gaggccgtag acgaggaaca tgaggctgcc ggtcaccgtg
     3001 accgcgcccc acgcgtcgaa gtgccgggcc atgccgtccg cgcggctctc ccggacgtag
     3061 cgcgggacga gcacgagcgc gccggccgcg atcgggaggt tcacgaagaa gatccactcc
     3121 caggagatgt acgaggtgag gacgccgccg agcagcacgc cgatcgcggc gcccgcgccg
     3181 gcgatgccgc cgaggatgcc gagcgccttg ttccgctcgg ggccctccgc gaacgtcacg
     3241 gacgtgatcg agaacacgga cggagcgagg atcgcgccgg ccgcgccctg ggcgagccgc
     3301 gcgatgatca gcatcgtgtc cgaggtggcg agcccggccc agagggacgt gacgaagaag
     3361 agggcgaggc cgaccatgaa gagcgtcctg cggccgagca ggtcgcccat gcgccctccg
     3421 aggaggagga accctccgaa cgtgagcgcg tacccggaga cgacccactg gaggttctcc
     3481 ggcgtgaagt tgagatccgt ctggatcgag gggagcgcga cgttcacgat ggagacgtcg
     3541 agcaccacca tgaggtaggc gatcccgagc acggcgagcg ccttccagcg gcgcggatcg
     3601 agtgcggtgt cttgcatgcg aggtctcctt ggggcgtttt ggttaccgag cactaacgat
     3661 gccgccgcga ggtctatgcc ggaatgcggc tggtccgagt tcgagggtgc ggatatccgc
     3721 accccgccgc gtcccggtta ggatcagcgg gtggagaccg tcgagcggat catgagccgc
     3781 gacctgctca ccgcggcgcc ggacgagcgg ctgcaggacg ccgcgaagcg gatggtcggg
     3841 cggcgcgtcg gcgcgatcct cgtgctcgag ggcgagcggc tcgccgggat cctgacggag
     3901 cgggacgtcc tccgcgcggt cgggaacggg ctcgaccccg acgcactcgt ctcgcagtgg
     3961 atgacgcgcg gcccggagac cgtcgagccg tccgcgaccg ccgaggaggc gctcgcgctc
     4021 atgcaccagg gcggcttccg gcacctgccg gtgaccgacg gggacaggct cgtcggaatc
     4081 gtgtcggtgc gcgacgtcct gcgcgtcggc gtccaggacg cctcgccgcg cggcgtctag
     4141 aacggcttga ccgtcatgac gaagaacgcg gccgtgagca gggccatgtc gatccaggag
     4201 agcgcgcgga tccgcgagat ctgatcctgg agctcctcgt cgggggcgtt cgcgacggcg
     4261 agcgccttga ggcggttccc ggcggggacg atgagcgcga tgccgaggac gaagacggcc
     4321 gcgagaagca cgatcccggc gacgacccag ccgtcgcgcc cgaggctcca cggcccctcg
     4381 accgtcagcc agatccccgc gccgagcacg acgagcgccg acgggatgaa gagaacctgc
     4441 gcgacccggt cctcgtactc gccgagcttg aggacgcccg gagtgtcccc gccgcggacg
     4501 gcgagctcca cgagcacgag ggagagcagc gcggccccga accacgccgc cgcggcgatc
     4561 acgtgtgcga agagcaagag ctcgtagagc gacacggccg cggagtatgg caggcgcgcg
     4621 tgcggtcgat ccacgagccg ggacgccggc cgcgcgacat tacgacggag ccggcggatc
     4681 gtcgatccgc cggctccttc cgtccgcgct acggcacgcc gcggatcgcg tccccgcggt
     4741 ccgcccgcgc cgtgtcgtgg ccccgcccgc cgtacacggc gtcgttcccg cgccggccgt
     4801 cccggctgtc gaggaagtcc gggccggggc ctccgtagag gcgatcccgc ccggccccgc
     4861 cgacgatctc gtcggcgccg ccgttgccgt agagcgtgtc gccgccgccc agcccgagga
     4921 tgagatccgc cccgggcgtg ccacggagga cgtcgcccgc cccggtcccc tgcaggatcg
     4981 cgccgggata ccagtgcagc tcgagcgagc cggtctcgcc gtcccagccc ccgacggcga
     5041 tcgcgtacgt cgtcccggcc tcggcgagga agctcacctc gctcgagtac cagccgaagt
     5101 cgtcgtttga gcacaggcac gtgagcgcgt cgaccgcggt gccggtgtag acgccgagca
     5161 cggtgtcgaa ctcgcttccg tgcgtgttga agcgaacgtg gccggtcacg ggcgccgtcc
     5221 agctgtacca gacggacgcc gttccgcccg cgacgagcgg ctcgcccggc tcgaccgtcg
     5281 ctccgatgtt cgacccgaac acgtctccgc gggcgccctc gaggagcgcc gcgtccgcga
     5341 agtcgtcgtt ctccgggttg acgaaccact ggagcccgaa gctcccggtg gctccgtcgt
     5401 agccgtcgac ggcgatccag agctccgtac cggccacgac gtcgaagacg acccggctcg
     5461 tgaacccgtc ccagtcgtcg ttcgacgcga cctcgacgag gtcgccgagc gtcgtgcccg
     5521 tgtagacggc gagcaccgga tcgaagcccc actcccatgc ggtgaagctc atggtttcgt
     5581 cgtggggagc gacccagcgg taccagagcg acgccccgcc ggagttgccg gcgtgcgccg
     5641 gctccccggg ctccttggtt gccttgaagt tcgccccgcc gacccagccg ttcggcccct
     5701 cgaggaccgt cgccgcggcg aatgagtcgt tctccgcggg atacgcggcg gcaacgcccg
     5761 tcaggacggc cgccacggtg atgaaggccg caagcgcggc cagaaatgtc gccagccgca
     5821 tcatcgtgtg ccccctttcc ttgccactga cagcgtctcg ccgcgctcgg cggacgtcat
     5881 cggggcggca ccgatctcgg cgccgaaact gcggatctac gccgtccggc ggcggagggt
     5941 cgtcgcgccg aggacgaggg cgagcacgat gcagccggtc acgaccgcga cgtcgagcca
     6001 gagccgcccg ccgatgtcgt cggcagccac gcgcacgagc ccgtcgaacg cgtacgtgaa
     6061 cg
//
LOCUS       DASTFC010000032         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_989324,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000032 DASTFC010000000
VERSION     DASTFC010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989324"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..769)
                     /locus_tag="VFO88_00995"
     CDS             complement(221..769)
                     /locus_tag="VFO88_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544130.1"
                     /translation="MATNTISRTLHDVGLALWSGGSTMGALGVNGAATAAEPKERLRV
                     AGEGWSRWQPAHMGAVAAYGLGSIGLTWGNRRRIRAQKGVAGLAIAKTAVTAAALAAD
                     AYAGRLGRRAAEAGDVPVESATEPASETPEDVANTLRRLKVVQWVVPALTGALVVLSA
                     RMGEQQRPQAVARGVRDRLLRR"
     gene            complement(875..1849)
                     /locus_tag="VFO88_01000"
     CDS             complement(875..1849)
                     /locus_tag="VFO88_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9544131.1"
                     /translation="MRRLAGLAALSLAALAVGGAGHAAPGAKPFTVCAKGCQYTTIQP
                     AVNAAADGATITVGPGVYAGGVTIEKNLTLVGVNPKRTVLAGTAGANVVEAVSGDITL
                     RGFTITGGDLGVSVSFFPRVTVVDSVITGNAQGSGGFGGFVGVYDSLVTRNSTAGVGP
                     IFSGGVSVEDSTVSDNGVGVLGDSRMLVVVRNSRILDNRNSGILMPGGTLEIYGSTIR
                     RNTGLNGGGVALYVGRAGSTATVVESTISHNTASAKGGGVFIDILSTLTLRNTKVFKN
                     TAQKGGGIYNGGEWQTEQNFHSIDSRIWNNTPDDYLEECDPARRACSP"
     gene            complement(1944..2645)
                     /locus_tag="VFO88_01005"
     CDS             complement(1944..2645)
                     /locus_tag="VFO88_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544132.1"
                     /translation="MSVERIREPRPAPVGAAAAERVFEWLSAERGSRIFHPQGTAYRG
                     VVRVDDGVDGVPLLAAGSTHDAVVRFSRGLGLPRGWPEFLGLALRIAGEQDLLLASSS
                     PSPLVRMVPLPARSFFGTTLSSLLPFDGDGRVVLVGALVHGTPTGDDDQLDEVANTPG
                     PVTADLLVAELGKPWRRFGGLELGERLPDEEARRLRFDPWNCAGGLVPHGWINRLRRP
                     AYRGSRRGRPSTATT"
     gene            complement(2690..>3128)
                     /locus_tag="VFO88_01010"
     CDS             complement(2690..>3128)
                     /locus_tag="VFO88_01010"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HET9544133.1"
                     /translation="PSSYLRLFFSDTIHMKVWLASAAAVLGVFQIFSAAWIFRKLPWS
                     RPRWINTAHRVSGAAALVLTLPVAYHCIFNLGFQDATARVLAHSLLGCVFFGAFAAKV
                     LVVELHRFPRWVLPTAGGLLFAVLVAVWYTSALWFFRLAGVGL"
BASE COUNT          473 a         1140 c         1086 g          429 t
ORIGIN      
        1 cagctcatgt acgaggtctt cgtgaacggc gtccgcgtga gctccgcgat cgggtacgac
       61 gaggacatcg tctactgcct cgacaccggg tcgaacgcga tccgggtgcg cgccgtggac
      121 acgtccggga acgccggacc gttctcgaac gagatcgtgc tcatctgctg agcgcgaccc
      181 gccgggggcc tgcggcggca caggcccccg gcggctcccc ctacctgcgg agaagcctgt
      241 cgcggacgcc gcgggcgacg gcctgcgggc gctgctgctc gcccatccgg gccgagagga
      301 cgacgagggc gccggtcagc gccgggacga cccactggac gaccttgagg cggcgcagcg
      361 tgttcgcgac gtcctccggc gtctcgctcg cgggctcggt cgcgctctcg accgggacgt
      421 caccggcctc ggcggcgcgg cgcccgagcc ggccggcgta ggcatccgcg gcgagcgcgg
      481 ccgcggtcac cgcggtcttc gcgatcgcga gccccgcaac gcccttctgc gcgcgaatcc
      541 ggcgccggtt gccccacgtg agcccgatcg agccgagccc gtacgcggcg accgcgccca
      601 tgtgcgcggg ctgccagcgg ctccagccct cgccggcgac gcgcagccgc tccttcggct
      661 cggcggccgt cgcggcgccg ttcacgccga gcgcgcccat ggtcgagcca cccgaccaga
      721 gcgccagtcc gacgtcgtgc agcgtcctag agatcgtgtt cgtggccatg gttactcctc
      781 cgttcgttga agtgccgcaa gccgttcctg taggcgccgc ccggcgcccg caaacacggg
      841 caccgggccg ccgcggctca tggctgcgcc tgggtcacgg agagcacgcc cgccttgcgg
      901 gatcgcactc ctcgaggtag tcgtccggcg tgttgttcca gatccggctg tcgatcgaat
      961 ggaagttctg ctcggtctgc cactcgccgc cgttgtagat gccgccgccc ttttgagccg
     1021 tgttcttgaa caccttcgtg ttgcgcagcg tcagcgtcga caggatgtcg atgaagacgc
     1081 cgccgccctt cgccgaagcg gtgttgtggc tgatcgtgct ctcgacgacc gtggcggtgc
     1141 tgcccgcgcg gccgacgtag agtgcgaccc cgccaccgtt cagcccggtg ttgcggcgaa
     1201 tcgtgctccc gtagatctcc agcgtgccac ccggcatgag gatgccggag ttgcgattat
     1261 cgagaatccg gctgttccgg acgacgacga gcattcgcga gtcgcccagc acgccgaccc
     1321 cgttgtccga aaccgtcgag tcctcgacgc tgacgccgcc gctgaagatc ggaccaacgc
     1381 ccgcggtcga gttccgggtg acaaggctgt catacacgcc gacgaagccg ccgaagccgc
     1441 ccgagccctg cgcgttcccc gtgatgacac tgtccacgac ggtcacccgc ggaaagaacg
     1501 agacgctgac gccgagatcg ccgcccgtga tcgtgaagcc gcggagcgtg atgtcgccgc
     1561 ttacggcctc gacaacgttg gctcctgcgg ttccggcgag caccgtccgt ttcggattga
     1621 caccgacgag cgtgaggttc ttttcgatcg tcacgccgcc tgcgtaaacg cccgggccga
     1681 cggtgatcgt cgccccatcc gccgcggcgt tcacggccgg ctggatcgtc gtgtactgac
     1741 agcctttcgc gcaaaccgtg aacggctttg ctcccggcgc cgcgtgtcca gcgcccccga
     1801 ccgccagcgc tgcgagagag agcgccgcga gccccgcaag ccttcgcatt ctccgatcgg
     1861 acaccgcctc cacctccggt tcggggtttg tgcccgaagt tacgacccgc ctcgcgagcg
     1921 ttcaagaggc ggaaccccgg ctctcatgtc gtggccgtgg agggccgtcc acgccggctg
     1981 ccccggtacg cggggcggcg caggcggttg atccagccgt gcgggacgag gccaccggcg
     2041 cagttccacg ggtcgaaccg gaggcgccgg gcctcctcgt cgggaagccg ctcgccgagc
     2101 tcgagcccgc cgaagcggcg ccacggtttg ccgagctcgg cgacgagcag gtcggcggtc
     2161 accggccccg gcgtgttcgc gacctcgtcg agctggtcgt cgtcaccggt cggcgtcccg
     2221 tggacgagcg cccccacgag gacgacgcgg ccgtccccgt cgaacggcag gaggctcgag
     2281 agcgtcgtcc cgaagaacga ccgggcgggc agcggcacca tccgcacgag gggcgacggc
     2341 gacgaggatg cgaggagaag atcctgctcg cccgcgatcc gcagcgcgag cccgaggaac
     2401 tccggccacc cgcgcggcag cccgagcccg cgcgagaacc gcacgaccgc gtcgtgcgtc
     2461 gagcccgcgg cgagcaacgg aacgccgtcc acgccgtcgt ccacgcgcac gaccccgcgg
     2521 taggcggtcc cctgcgggtg gaagatccgg ctcccgcgct cggccgagag ccactcgaag
     2581 acgcgctcgg cggcagcggc ccccacggga gccggccggg gctctcggat ccgctcgacg
     2641 ctcacgcccg ttcagttccg ccgcacgcgt gcgccaaacg gcgctcgctc tagagaccga
     2701 cgccggcgag ccggaagaac cagagcgcgc tcgtgtacca gacggcgacc aggacggcga
     2761 agagcagccc gcccgccgtc gggaggaccc agcgcgggaa ccggtgcagc tcgacgacga
     2821 gcaccttggc cgcgaacgct ccgaagaaga cgcagccgag cagcgagtgg gcgagcacgc
     2881 gggcggtcgc atcctggaag cccaggttga agatgcagtg gtacgcgacg ggcagcgtga
     2941 ggacgagcgc agccgctccc gagacccgat gcgccgtgtt gatccaccgc ggccgcgacc
     3001 agggcagctt ccggaagatc caggcggccg agaagatctg gaacacgccg agcacggccg
     3061 ccgcgctcgc gagccagacc ttcatgtgga tcgtgtcgct gaagaagagc cggaggtagc
     3121 tcgacggc
//
LOCUS       DASTFC010000033         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1005863,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000033 DASTFC010000000
VERSION     DASTFC010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005863"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFO88_01015"
     CDS             complement(<1..830)
                     /locus_tag="VFO88_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HET9544134.1"
                     /translation="MEGPREEGGRGLGPWLPEPPPPLPPGYTPPVAPDEPPPFRPVRR
                     RARWAAGILAVSFVADVVAVALGLSERGLLTRLVDGEVVPPDELSASDDRQLGIGLAQ
                     AIVLVAAAVAFLAWFFRAYTNLEALGVQRLRYGRRWSVGAWFVPILNLFRPKQIANDI
                     WRGSDPDAAERPLAGIGSVPALFWFWWAAFLLSGALYNAAARAELEAVVPEALLTTNG
                     LWLAADVVGAISAPLAIAVVLRTTSRQERRAARLGLATVPFPTPLLRRGTTWAAAALT
                     L"
     gene            complement(958..1869)
                     /locus_tag="VFO88_01020"
     CDS             complement(958..1869)
                     /locus_tag="VFO88_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9544135.1"
                     /translation="MLLLLLALASLAFAVYLAGEVATLPARQRQGSIRRAANYGHSRR
                     HVNPFAVNTSFRERALGPAATGLARMVLRVSPRSTVDSINLKLHGAGLSRRLSPTSFL
                     ATKGVLAASGLVVGLLAGAGEGVSGKALLLALGVAFAGFMFPDTIITFRARSRRERIQ
                     AELPDALDLLAVSVEAGLGFDGAITKLTEHMHGPLAEEFALSLGEMRIGESRQVSLKR
                     MADRVNSPAMAAFTRAVIQADQLGISLGRILRLQAAEARNRRQATAEERAMKAPIKML
                     FPTVIFIFPAMFIVILGPAFLNLSKIF"
     gene            complement(1874..>2669)
                     /locus_tag="VFO88_01025"
     CDS             complement(1874..>2669)
                     /locus_tag="VFO88_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9544136.1"
                     /translation="MAASLFRATERTFGHRDFWSRIQTKLDRADLPLRTVEFLYVVGG
                     SSLAFGIVAALVGPPMPFVLGAFGLGALLPFGFVSFRAKRRLKAFDAQLPELLTTIAA
                     SLKAGHSFRQGIQAVVEEGQEPAAGEFKRVLAETRLGRPMDAALAEMSRRVGSKNLEF
                     VLTAVTIQRQVGGSLAAIFDMVAETVRNRHQFARKVKGLTAMGRASAYVLVGLPFFVA
                     LAVTFVNAEYMAPLYYTSTGRMLIGIGLTMIAIGSLILRKMVSFRS"
BASE COUNT          421 a          950 c          989 g          309 t
ORIGIN      
        1 agcgtgaggg ccgccgccgc ccacgtcgtg ccgcggcgga gcagcggcgt cggaaacgga
       61 acggtcgcaa gcccgaggcg cgccgccctc cgctcctgcc ggctcgtcgt gcgcagcacg
      121 accgcgatcg cgagcggtgc cgagatcgcg ccgacgacgt cggcggcgag ccagagcccg
      181 ttcgtcgtca ggagcgcctc cggtacgacc gcttccagct ccgcgcgggc ggccgcgttg
      241 tagagggcgc cgctcaggag gaacgcggcc caccagaacc agaagagcgc cgggacggag
      301 ccgattccgg cgagcggccg ctccgccgcg tccgggtcgc tgccgcgcca gatgtcgttc
      361 gcgatctgct tcgggcggaa gaggttcagg atcgggacga accacgcgcc gaccgaccag
      421 cgccgcccgt agcggagccg ctggacgccg agcgcctcga ggttcgtgta cgcgcggaag
      481 aaccaggcga ggaacgcgac cgccgcggcg acgagcacga tcgcctgcgc gagcccgatc
      541 ccgagctgcc ggtcgtcgct cgccgagagc tcgtccggcg ggacgacctc gccgtcgacg
      601 agccgcgtca ggaggccgcg ctcgctcagg ccgagcgcga ccgcgacgac gtcggcgacg
      661 aacgagaccg cgaggatccc cgccgcccag cgcgcccgcc ggcgaaccgg gcggaacggc
      721 ggcggctcgt ccggcgcgac gggcggtgtg tagccgggcg gaagcggcgg cggcggctcc
      781 gggagccacg gtccaaggcc gcgccccccc tcctcccgcg gtccctccac gtatcgaacc
      841 gtacaacggc ttccgcccgg cgtcgatgcc ccgttcgcgg gcgggcttcg cccgccgcgg
      901 gtcacgaagc ggcgcgagag cccctgcgag ccggaggccg gccgcgagcg tggagagcta
      961 gaagatcttc gagaggttga ggaaggccgg cccgaggatg acgatgaaca tcgccgggaa
     1021 gatgaagatc accgtcggga agagcatctt gatcggcgcc ttcatggccc gctcctcggc
     1081 ggtcgcctga cggcggttcc gcgcctcggc ggcctgcagg cgcaggatcc gcccgagcga
     1141 gatgccgagc tggtcggcct ggatgaccgc gcgcgtgaac gccgccatcg cgggcgagtt
     1201 cacgcggtcg gccatcctct tcagcgagac ctgccggctc tcgccgatgc gcatctcgcc
     1261 gagcgagagc gcgaactcct ccgcgagcgg gccgtgcatg tgctcggtca gcttcgtgat
     1321 cgcgccgtcg aacccgagac ccgcctcgac gctcaccgca aggaggtcga gcgcgtccgg
     1381 gagctccgcc tggatccgct cgcggcggct cctcgcgcgg aaggtgatga tcgtgtccgg
     1441 gaacatgaac ccggcgaagg cgacgccgag cgccaggagg agcgccttgc ccgacacgcc
     1501 ctcccccgcg ccggcgagca gcccgaccac gagcccgctc gccgcgagca cgcccttcgt
     1561 ggcgaggaac gaggtcggag agagccgccg cgagagcccc gcgccgtgca gcttcaggtt
     1621 gatcgagtcg accgtggaac gcgggctcac acggagcacc atgcgcgcga ggccggtcgc
     1681 ggccggcccg agcgcccgct cgcggaagct cgtgttgacg gcgaacgggt tcacgtgccg
     1741 gcgcgagtgc ccgtagttcg cggcgcgccg gatcgagccc tggcgctggc gggcgggcag
     1801 cgtcgcaacc tcgccggcca ggtagacggc gaaggcgagc gacgcgagtg cgaggagaag
     1861 cagcagcatc tgatcagctc ctgaacgaga ccatcttgcg gaggatgagc gagccgatcg
     1921 cgatcatcgt gagcccgatc ccgatcagca tgcggcccgt ggacgtgtag tagagcggcg
     1981 ccatgtactc ggcgttcacg aaggtgaccg cgagcgcgac gaagaacggc agcccgacga
     2041 ggacgtaggc cgaggcccgc cccatggcgg tcaggccctt caccttgcgc gcgaactggt
     2101 gccggttgcg caccgtctcg gcgaccatgt cgaagatcgc ggcgagcgag ccgccgacct
     2161 ggcgctggat cgtcaccgcg gtgaggacga actcgaggtt cttcgagccg acccggcggg
     2221 acatctccgc gagcgcggcg tccatcgggc ggccgaggcg cgtctcggcg agcacgcgct
     2281 tgaactcgcc cgcggcgggc tcctggccct cctcgacgac cgcctggatg ccctgacgga
     2341 agctgtgccc ggccttgagc gaggcggcga tcgtcgtcag gagctcggga agctgcgcgt
     2401 cgaacgcctt caggcggcgc ttcgcccgaa acgagacgaa cccgaacgga agcagcgcgc
     2461 cgagcccgaa cgcgccgagc acgaacggca tgggcggccc gacgagcgcc gccacgatcc
     2521 cgaaggcgag ggacgagccg ccgacgacgt agaggaactc gacggtccgg agcggcaggt
     2581 cggcgcggtc gagcttcgtc tggatccggc tccagaagtc ccggtggccg aacgtccgct
     2641 cggtcgcgcg gaagaggctc gcggccatc
//
LOCUS       DASTFC010000034         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_636,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000034 DASTFC010000000
VERSION     DASTFC010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VFO88_01030"
     CDS             complement(<1..1788)
                     /locus_tag="VFO88_01030"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET9544137.1"
                     /translation="MATPRTAPPRIQIANLWPELACGRYPVKRTQGSTVEVWADVFRD
                     GHETLGAAVRYRAPGARRWREAPMRHVGADRWTAAFDADELGRWAFQVEAWVDRVATW
                     QDELRRKHEAGQTELAGELSEGAALLGLERLTLAEGLAAETGDRSEVTRSATREVDVD
                     RELAAFGSWYELFPRSWGGLAGVAEIVPELAELGFDVVYLPPVHPIGETNRKGKNNAL
                     TARRGDPGSPWAIGSSAGGHTALEPSLGTFDDFERLVAAAREHDMEVALDFAIQCSPD
                     HPWLEEHPDWFHRRPDGTLKYAENPPKKYQDIYNVNFDSPDWRALWEALRDVVLFWVE
                     RGVTVFRVDNPHTKPLPFWEWLIREVRAEHPEVVFLSEAFTRPAMMQTLALAGFGQSY
                     TYFTWRTTKAELTEYMVELSHSLQPQYFRPNFFVNTPDILTEELQRGGLPAFAARAVL
                     AATLSPSWGIYSGFEHGENVPAGEGSEEYLSSEKYEVKERRLDGPLLPLIGRLNRIRR
                     ENPAFRRIDNLTFLETENDRLIAYAKREGRNVVVTCVNLDHFAPQEGVVVIPAELGLP
                     PAFRARELLTDATFDWRIGRNYVRLDPAAP"
     gene            1835..>2427
                     /locus_tag="VFO88_01035"
     CDS             1835..>2427
                     /locus_tag="VFO88_01035"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET9544138.1"
                     /translation="MRSAGILLHPTSLPSGRLDGEAYRFVDWLAAAGQRWWQVLPLGP
                     PGEGRSPYTSPSAFAGSPELLADPSAPVSAREEWAFRGREAAWADGWAEHAGDGALAD
                     QVRFEREWQALRAYARARGISILGDVPIYVARGGADHLAHRELFVEGEVAGAPPDAFS
                     DVGQLWGNPLYDWRANRADGYRWWIERFRRVLSLVDAA"
BASE COUNT          303 a          841 c          930 g          353 t
ORIGIN      
        1 cggcgcggcg gggtcgaggc gcacgtagtt ccgtccgatc cgccagtcga acgtcgcgtc
       61 cgtgagcagc tcgcgggcgc ggaacgccgg cggcagcccg agctcggccg ggatcacgac
      121 gacgccctcc tgcggcgcga agtggtcgag gttcacgcac gtcacgacga cgttacggcc
      181 ctcgcgcttc gcgtaggcga tcaggcggtc gttctccgtc tcgaggaacg tgaggttgtc
      241 gatccgccgg aaggcggggt tctcgcgccg gatgcggttc agccgcccga tgagcggcag
      301 gagcggcccg tcgagccggc gctccttcac ctcgtacttc tccgagctca ggtactcctc
      361 gctcccctcc cccgccggca cgttctcgcc gtgctcgaag ccggagtaga tcccccagct
      421 cggcgagagc gtcgccgcga gcaccgcgcg cgccgcgaac gcgggcaggc cgccgcgctg
      481 gagctcctcc gtgaggatgt cgggcgtgtt gacgaagaag ttggggcgga agtactgcgg
      541 ctggagcgag tgcgagagct cgaccatgta ctcggtgagc tcggccttcg tcgtgcgcca
      601 cgtgaagtac gtgtagctct ggccgaaccc ggcgagtgcg agcgtctgca tcatcgccgg
      661 gcgcgtgaac gcctcggaga ggaagacgac ctcgggatgc tccgcgcgca cctcgcggat
      721 gagccactcc cagaacggga gcggcttcgt gtgcgggttg tcgacgcgga agaccgtgac
      781 cccgcgctcg acccagaaga ggacgacgtc gcggagcgcc tcccagagcg cccgccagtc
      841 gggcgagtcg aagttgacgt tgtagatgtc ctgatacttc ttgggcgggt tctcggcgta
      901 cttcagcgtc ccgtcggggc ggcggtggaa ccagtccggg tgctcctcga gccaggggtg
      961 gtcgggggag cactggatcg cgaagtcgag cgcgacctcc atgtcgtgct cgcgggcggc
     1021 ggcaacgagg cgctcgaagt cgtcgaacgt cccgagcgac ggctcgagcg ccgtgtggcc
     1081 gcccgccgag gagccgatcg cccacgggct gccgggatcg cccctgcgcg cggtgagcgc
     1141 gttgttcttt cccttgcggt tcgtctcgcc gatcgggtgg acgggcggaa ggtagacgac
     1201 gtcgaagccg agctcggcga gctcggggac gatctccgcc acgccggcga ggccgcccca
     1261 cgagcggggg aagagctcgt accaggagcc gaaggcggcg agctcgcggt cgacgtccac
     1321 ctcgcgggtc gcggagcgcg tgacctccga gcggtcgccc gtctcggcgg cgaggccctc
     1381 ggcgagcgtg aggcgctcga ggccgagcag cgcggcgccc tcggagagct cgccggcgag
     1441 ctccgtctgg ccggcctcgt gcttgcggcg cagctcgtcc tgccacgtcg cgacgcggtc
     1501 gacccaggcc tcgacctgga acgcccagcg gccgagctcg tcggcgtcga aggcggccgt
     1561 ccagcggtcg gcgccgacgt ggcgcatcgg agcctcgcgc cagcgccgcg cgccgggcgc
     1621 ccggtagcgg accgccgcgc cgagcgtctc gtggccgtcg cggaagacgt ccgcccagac
     1681 ctcgaccgtc gagccctgcg tgcgcttcac ggggtagcgg ccgcacgcga gctcgggcca
     1741 gaggttcgcg atctggatgc gcggcggtgc cgtccggggg gtcgccatgt tctgtgtcct
     1801 gtcctatccg cgtcggttgg ggtacacacg cgggatgcgc tcggccggaa tcctgctgca
     1861 tccgacgtcc ctgccgtcgg gccgactgga cggcgaggca taccggttcg tcgactggct
     1921 cgcggcggcg gggcagcggt ggtggcaggt gctgccgctc gggccgcccg gagaagggcg
     1981 ttcgccgtac acctcgccgt cggcctttgc gggctcgccg gagcttctcg ccgacccgtc
     2041 ggcccccgtc tcggcgcggg aggagtgggc gttccgcggg cgtgaggccg cgtgggcgga
     2101 cgggtgggcg gagcacgccg gcgacggcgc gctcgccgac caggtgcgct tcgagcgcga
     2161 gtggcaggcg ctgcgcgcgt acgcgcgcgc gcgcgggatc tcgatcctcg gcgacgtgcc
     2221 gatctacgtc gcgcgcggcg gcgccgacca cctcgcgcac cgggagctct tcgtcgaggg
     2281 cgaggtcgcg ggcgcgcctc cggacgcgtt cagcgacgtg ggccagctct ggggcaaccc
     2341 gctctacgac tggcgggcga accgcgccga cggctaccgg tggtggatcg agcgcttccg
     2401 gcgcgtgctc tcgctcgtcg acgccgc
//
LOCUS       DASTFC010000035         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_676330,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000035 DASTFC010000000
VERSION     DASTFC010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676330"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFO88_01040"
     CDS             complement(<1..543)
                     /locus_tag="VFO88_01040"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544139.1"
                     /translation="MAADELVVHGAREHNLKDITVRLPRNALICVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLQMMEKPDVDHIDGLSPAISIDQKTTSRNPRSTVGT
                     VTEIYDYLRLLYARVGRPHYPVCGRPISGQSLESIVDQVLQLPEGTRFTVNAPVVRDR
                     KGEYREVLEELRREGFTRVKV"
     gene            complement(590..1651)
                     /locus_tag="VFO88_01045"
     CDS             complement(590..1651)
                     /locus_tag="VFO88_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9544140.1"
                     /translation="MRLARTLRLCGLGALALALAAPVSASAQTPRPGQLALATNGGMH
                     ELSDEEIAAELDVARDAGARWVRFDLNWAVIQEEGPESWRWEPFDRMIEGARARGLNV
                     LGTIWYTPAWARPSDTPAHRPPTVVEDYARFAAAAAERYMALGVHHWEIWNEPNIPQA
                     WAGGVSAKNYGLLLAAAASAIRAVDSEAVILSAGLSPAATRGGSLAPPEFLRRLYRHG
                     FASSFDQVGHHASTFPHLPSRKKDWSAWQQMAWTKPSLRSVMRRYGDAKKRIWITELG
                     APTGGDRAVSEARQARILTEAHRLWAGYRWAGPLFWYAVRDRGPVTWHNTCGLVREDL
                     TPKLSFAAYRAIANRARLR"
     gene            complement(1685..>2215)
                     /locus_tag="VFO88_01050"
     CDS             complement(1685..>2215)
                     /locus_tag="VFO88_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544141.1"
                     /translation="AGARGIRRANRRAVIITGGLSPAANADGNIAPVTFLSRLYRHGF
                     RRSFDHVGHHPYTFPSYPSRRRAWSPWRQMAWTRPSLRAVMVKFGNRHKRIWATEYGA
                     PTGSDRAVSESTQAAMLREAYRLWGRYSWTGPLFWYSLRDRGPVSWHTTCGLVRGDFS
                     RKPAFAAYRAIADSSR"
BASE COUNT          297 a          775 c          833 g          310 t
ORIGIN      
        1 gaccttgacg cgcgtgaagc cctcgcgccg gagctcctcg agcacttccc ggtactcgcc
       61 cttgcggtcg cggacgaccg gcgcgttgac ggtgaagcgc gtcccctcgg ggagctggag
      121 cacctggtcg acgatcgact cgaggctctg cccggagatc ggccgcccgc agacggggta
      181 gtgcggccgg ccgacgcgcg cgtagaggag gcggaggtag tcgtagatct cggtgaccgt
      241 gccgaccgtc gagcgcgggt tgcgcgaggt cgtcttctgg tcgatcgaga tcgccggcga
      301 gagcccgtcg atgtggtcga cgtcgggctt ctccatcatc tggaggaact ggcgcgcgta
      361 cgcggagagc gactcgacgt agcggcgctg gccctccgcg tagatcgtgt cgaaggcaag
      421 gctcgacttc ccggacccgg agagtccggt cacgcagatg agggcgttcc gcggcagccg
      481 gacggtgatg tccttgaggt tgtgctcgcg ggcgccgtga acgacgagct cgtcggccgc
      541 catacgggcc agtgtagttt ccggcccgga cgaacgcatg ttcgggtcgt tagcggagcc
      601 gggctcggtt cgcgatcgcg cggtacgcgg cgaaggacag cttgggggtg agatcctcgc
      661 gcacgagccc gcacgtgttg tgccacgtca ccggcccgcg gtcgcgcacg gcgtaccaga
      721 agagcggtcc ggcccaccgg tagcccgccc agaggcggtg cgcctcggtg agaatccgcg
      781 cctgccgcgc ctcgctcacg gcgcggtcgc cgccggtcgg ggccccgagc tcggtaatcc
      841 agatccgctt cttcgcgtcg ccgtagcgtc gcatcacgga gcgcaggctc ggcttcgtcc
      901 acgccatctg ctgccacgcg ctccagtcct tcttccgcga cggcaggtgc gggaaggtcg
      961 acgcgtggtg cccgacctgg tcgaaggacg acgcgaagcc gtggcggtac agccggcgga
     1021 ggaactcggg cggcgcgagg ctccccccgc gcgtcgcggc cggggagagg ccggcgctca
     1081 ggatgaccgc ctccgagtcg acggcgcgga tcgccgacgc ggcggccgcg agcagcaggc
     1141 cgtagttctt cgcgctcacg ccgccggccc acgcctgcgg gatgttcggc tcgttccaga
     1201 tctcccagtg gtggacgccg agcgccatgt accgctcggc ggcggccgcg gcgaaccgcg
     1261 cgtagtcctc gacgaccgtc ggcggccggt gcgccggcgt gtcagacggc cgcgcccagg
     1321 cgggtgtgta ccagatcgtc ccgaggacgt tcaggccgcg cgcccgggcg ccctcgatca
     1381 tgcggtcgaa cggctcccag cgccacgact cgggcccttc ctcctggatg acggcccagt
     1441 tcaggtcgaa ccggacccag cgcgcgccgg cgtcgcgggc gacgtcgagc tccgccgcga
     1501 tctcctcgtc ggagagctcg tgcatccccc cgttcgtcgc cagggcgagc tgccccgggc
     1561 ggggcgtctg cgccgacgcg gagaccggcg cggccagggc gagcgcgagc gcgccgaggc
     1621 cgcagagccg cagggttcga gcgagccgca cggcaccctt gtagcagagc cgggccgggg
     1681 agcgctaccg cgaggagtcc gcgatcgcgc ggtaggcggc gaacgcgggc ttgcgggaga
     1741 agtctccccg gacgaggccg cacgtcgtgt gccagctgac ggggccgcgg tcgcgcagcg
     1801 agtaccagaa gagcggcccg gtccacgagt accgccccca gaggcggtag gcctctcgca
     1861 gcatggccgc ctgcgtggac tcgctcacgg cgcgatcgct gcccgtgggc gccccgtact
     1921 cggtcgccca gatcctcttg tgccggttgc cgaacttgac catgacggca cggaggctcg
     1981 gcctcgtcca ggccatctgc cgccacgggc tccaggcgcg ccggcgcgac gggtagctgg
     2041 ggaacgtgta ggggtggtgg ccgacgtggt cgaacgaccg cctgaacccg tgccggtaga
     2101 gacggctcag gaacgtgacg ggcgcgatgt tgccgtccgc gttcgccgcc ggggacaatc
     2161 cgcccgtgat gatgaccgcg cgccggttgg cccgccggat gccgcgggcc cccgc
//
LOCUS       DASTFC010000036         4889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_99505,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000036 DASTFC010000000
VERSION     DASTFC010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99505"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFO88_01055"
     CDS             complement(<1..302)
                     /locus_tag="VFO88_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544142.1"
                     /translation="MGSVGALAFLLGRGRLLVAAGLLALGVAEALVAQDLLPDGIELG
                     SAVGLAALAVGAPLLALLAVLFVHRPAVLVPAVVGTAWLRWPFEIDTTDGLSIGLG"
     gene            469..1896
                     /locus_tag="VFO88_01060"
     CDS             469..1896
                     /locus_tag="VFO88_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9544143.1"
                     /translation="MKKRILIAVALVLLAAVGTAGVFAALWYREQTEPKNLRGSSTVE
                     FVTTEEPGEELRPEREVERVPWPMFGYDLARTKVAPDFEHRPPFRRLWGVETRDYIEF
                     PPAVANGRVFVANQRGVFLAIRTRNGKVMWRKELGRCVASGPAFSHGIVYLGFGTTKP
                     CGRSENRAGQSGGVIAVNARTGKVHWRFDAAPVESSPLVVGNLVYVGSWNERVYALNR
                     KSGSVRWAYNAGEEVNSSAAYDDNTIYIGGEEGTLFALNAWTGALRWRASSYSRFGRR
                     EQFYPTPVVAYGRVFAANVDGTVYAFGAKSGRLLWARTAGTYVYTSPAVWRNRVYVGS
                     YDGKFSALDAATGDIVWQWEAPGAIHGAPTIMAGLVYFSTTSSGVATSKAQRFIKAGR
                     RGSYALDARTGKPVWELPNVGQYSPVVADEAHVYLTGSTRVYGMLARNTARALRREER
                     RERREREREEREREERSDAPEADGS"
     gene            complement(1910..2737)
                     /locus_tag="VFO88_01065"
     CDS             complement(1910..2737)
                     /locus_tag="VFO88_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544144.1"
                     /translation="MDTLVAERARMIADDRTHGAGWLARNAVEALAEAVARGADPLES
                     GRALVSARPAMGAIAGAVGRVLAAGRTPELLLEECRALVDRRDRAAHSIAVLLKPFLL
                     SRVMTHSSSATVREALLYSEPSRVVCTVARPYDEGRAFADELRETGLAVDLVAEEDAG
                     HALETVDVLLLGADCVFRDGAFANRVGTRELAATATERGVPVLVAFETFKLAPFDTPG
                     PGEDDWEDEEPFDLTPAEYVDRYVTDEGDFDPADIAALVDQTPFLKEGWELLSGSRR"
     gene            2877..3875
                     /locus_tag="VFO88_01070"
     CDS             2877..3875
                     /locus_tag="VFO88_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9544145.1"
                     /translation="MRRFRYPLQILVSVGILAFLLYHIDVGRTVELVRESDVRYVALA
                     LAIFVVTTWVMAWRWQLLLASKGIREPLGWLTKLYFVGYAAGQVLPTSMGGDAVRIVE
                     HARRRPGARAEAAGAVVIERVIGATGTLLLVAVGLVVAAGRYSDLEAVLWVELAGLLT
                     VLGLGVLIFSRRTRRLLEERIFPLGRRVRLEKPLERVHLALHGYRDQRGVLAFTLALT
                     VVTQGARLVAIWMCGEAVGVDLSPLVYVILGPLLFLVMLVPFTINGFGVREAFFVAFL
                     GRLGVESDAAFATGFLFYVVTVLTSVPGAVILLWRSLPPAVRGRVRPGVGPDGSAS"
     gene            3886..>4889
                     /locus_tag="VFO88_01075"
     CDS             3886..>4889
                     /locus_tag="VFO88_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544146.1"
                     /translation="MQALERYLASRPMPPAVVADVGWVNGLAAIRQLARAGIPVIALD
                     QRKTALGFRSRYGFAVLAPDPLTEREAYVELLLRLGDAIGRPAPVFPTHDDHLNAIAG
                     AAGRLGERFRYPFPAWDVLEQVQRKRHQLERAVELGVPTPRTASGPTSDLGFPVLVKP
                     SDPVEFRRVFKRQSFRCETRAELDEAWERARPYDPLVQELIPGGDEELYTLGSYLSAD
                     GEALGLFCGRKLRQTPAGVGTARIGEAVWVDEVVEQGLALVRGLGAHGIAQTEFKRDP
                     RDGVFKLMEVNPRLWQWHGLAAACGVDLPRIAYWDLLGARLPPARSNGTRKRWAITHV
                     A"
BASE COUNT          627 a         1734 c         1846 g          682 t
ORIGIN      
        1 tcgccgagcc cgatcgagag cccgtcggtc gtgtcgatct cgaacggcca gcgcagccac
       61 gccgtgccca cgactgcggg cacgagcacc gcggggcggt ggacgaacag gacggcgagg
      121 agcgcgagga gcggggcccc gaccgcgagc gccgcgagcc cgacggccga gccgagctcg
      181 attccgtccg gaagcagatc ctgcgccacg agcgcctcgg cgacgccgag cgcgaggagc
      241 cccgcggcga cgagcaggcg gccccggccg agcaggaacg cgagcgcccc gactgacccc
      301 acaagcgccg cggcagtcgt catcgcgccg tgccgcgcgt gtcgtcggcg actgaggcgg
      361 cctgacggga cacgcgggta acgtatccgc aaggcgcgcc cggatcagcc gacaagcggc
      421 gcacgtcgac cggacggcgg gacaccgccg ccgcgctcct ctcgcccggt gaaaaagcgg
      481 atcctcatcg ccgtagcgct cgtgctgctc gccgccgtcg gcacggccgg cgtcttcgcc
      541 gcgctctggt accgcgaaca gacggagccg aagaacctcc gcggctcgtc cacggtcgag
      601 ttcgtgacga cggaggagcc cggcgaggag ctgcggcccg agcgcgaggt cgagcgcgtg
      661 ccgtggccga tgttcggcta cgacctcgcg cggacgaagg tcgcgccgga cttcgagcac
      721 aggcctccct tccggcggct ctggggggtc gagacgcggg actacatcga gttcccgcct
      781 gcggttgcga acgggcgggt cttcgtggcg aaccagaggg gcgtcttcct ggcgatccgc
      841 acccgcaacg ggaaggtcat gtggcggaag gaactcgggc gctgcgtcgc gtccggcccg
      901 gctttctcgc acgggatcgt ctatctcggc ttcggaacga cgaagccctg cgggcggagt
      961 gaaaaccgcg cggggcagtc cggcggcgtg atcgcggtga acgcgcggac cgggaaggtg
     1021 cactggcgct tcgacgcggc gccggtcgag tcgtcgccgc tcgtggtcgg caacctcgtc
     1081 tacgtcggat cgtggaacga gcgggtctac gccctgaaca ggaagagcgg ctccgtgcgc
     1141 tgggcgtaca acgcgggcga ggaggtgaac agctccgccg cgtacgacga caacacgatc
     1201 tacatcggcg gcgaggaggg gacgctcttc gcgctgaatg cgtggacggg agcgctcagg
     1261 tggcgtgcga gctcgtactc gcgcttcggg cggcgcgagc agttctaccc gaccccggtc
     1321 gtcgcgtacg ggcgggtgtt cgcggcgaac gtggacggga cggtgtacgc gttcggcgcc
     1381 aagagcgggc ggctgctctg ggcacggacg gcggggacgt acgtctacac ctcccccgcg
     1441 gtctggcgga accgggtcta cgtcggctcc tacgacggga agttcagcgc gctcgacgcg
     1501 gccaccggcg acatcgtctg gcagtgggag gcgccgggcg cgatccacgg cgcgccgacg
     1561 atcatggccg gcctcgtgta cttctcgacg acgtcgtccg gcgtcgccac gtcgaaggcg
     1621 cagcgcttca tcaaggccgg ccgccgcggc tcgtacgcgc tcgacgcgcg cacgggcaag
     1681 ccggtctggg agctcccgaa cgtcggccag tactcccccg tggtcgcgga cgaggcgcac
     1741 gtgtacctga ccgggtcgac gcgcgtgtac gggatgctcg cgcgcaacac cgcccgcgcg
     1801 ctcaggcggg aggagcggcg ggagcgccgc gagcgggagc gcgaggaacg ggagcgcgag
     1861 gagcgctccg acgcgccgga ggcggacggc tcgtagcgcc cggggcgcgt cagcgccgcg
     1921 agccgctgag gagctcccag ccctccttca ggaacggggt ctggtcgacg agcgcggcga
     1981 tgtcggccgg gtcgaagtcg ccctcgtcgg tcacgtagcg gtcgacgtac tcggccgggg
     2041 tgaggtcgaa cggctcctcg tcctcccagt cgtcctcgcc cgggccggga gtgtcgaacg
     2101 gcgcgagctt gaacgtctca aaggcgacga ggacggggac cccgcgctcg gtggcggtcg
     2161 ccgcgagctc tcgcgtcccg acccggttcg caaaggcgcc gtcgcggaag acgcagtcgg
     2221 cgccgagcag caggacgtcg acggtctcga gcgcgtgccc ggcgtcctcc tctgccacga
     2281 ggtcgaccgc gaggcccgtc tcgcggagct cgtcggcgaa cgcccgcccc tcgtcgtagg
     2341 ggcgggcgac ggtgcagacg acgcgcgaag gctcgctgta gaggagcgcc tcccgcacgg
     2401 tcgcggacga ggagtgcgtc atcacgcggc tgaggaggaa cggcttcagg aggacggcga
     2461 tcgagtgcgc cgcccggtcg cggcggtcga cgagcgcgcg gcactcctcg agcaggagct
     2521 ccggcgtgcg cccggccgcc aggacacggc ccaccgcgcc cgcgatcgcg cccatcgccg
     2581 ggcgcgcgga aacgagcgcg cggccgctct cgagcgggtc ggctccgcgg gccaccgcct
     2641 cggcgagggc ctcgacggcg ttccgcgcga gccagcccgc accgtgcgtg cggtcgtccg
     2701 cgatcatcct ggcgcgctct gcgacgagcg tgtccaccct cacctcctgc ccgttcaccc
     2761 tacctccccg ccgacggcgt ctggcacgca tgggccgctg gtagcctcct gcgggtgcac
     2821 cgggccgcgg agcggatcgt cctcccacgc ggccggtccg cccctcggag cgcatcgtga
     2881 ggcgcttccg gtaccccctc cagatcctcg tcagcgtggg gatcctcgcg ttcctcctct
     2941 accacatcga cgtggggcgg acggtcgagc tcgtgcggga aagcgacgtc cgctacgttg
     3001 ccctcgcgct cgcgatcttc gtcgtgacga cctgggtgat ggcgtggcgg tggcagctcc
     3061 tgctcgcgtc gaaggggatc cgggagccgc tcggctggct cacgaagctc tacttcgtcg
     3121 ggtacgcggc ggggcaggtg ctgccgacct cgatgggcgg ggacgcggtg cggatcgtcg
     3181 agcacgcgcg tcgccggccg ggcgcgaggg ccgaggcggc cggcgcggtc gtgatcgagc
     3241 gcgtgatcgg ggcgacgggg acgctcctcc tcgtcgccgt cggtctcgtc gtcgcggcgg
     3301 ggcgctactc ggatctcgag gccgtgctct gggtggagct cgcggggctc ctgaccgtgc
     3361 tcgggctcgg tgtgctcatc ttctcgcggc ggacgcggcg gctcctcgag gagcggatct
     3421 tcccgctcgg ccgccgcgtg cggctcgaga agccgctcga gcgcgtccac ctcgcgctgc
     3481 acgggtaccg cgaccagcgc ggcgtgctcg cgttcacgct cgcgctcacg gtcgtgacgc
     3541 agggcgcccg gctcgtggcg atctggatgt gcggggaggc ggtcggcgtc gacctctcgc
     3601 cgctcgtgta cgtgatcctc gggccgctgc tcttcctcgt catgctcgtc ccgttcacga
     3661 tcaacgggtt cggcgtgcgc gaggcgttct tcgtcgcgtt cctcgggcgg ctgggggtcg
     3721 agagcgacgc cgcgttcgcg accggctttc tcttctacgt cgtgacggtg ctgacgtccg
     3781 tgccgggcgc ggtcatcctc ctgtggcgca gcctgccgcc ggcggtgcgc ggccgcgtgc
     3841 ggcccggtgt cgggccggac ggctcggctt cctagaatcc cggacgtgca ggcgctcgag
     3901 cgctacctcg cgagccgccc catgcctccc gcggtcgtgg ccgacgtcgg ctgggtgaac
     3961 gggctcgccg cgatccgcca gctcgcgcgg gccgggatcc ccgtgatcgc gctcgatcag
     4021 cgcaagaccg cgctcggctt tcgctcgcgc tacggcttcg ccgtgctcgc gccggacccg
     4081 ctcaccgagc gcgaggccta cgtggagctg cttttgcggc tcggcgacgc gatcgggcgg
     4141 cccgcgcccg tgttcccgac gcacgacgac cacctgaacg cgatcgccgg cgcggccggg
     4201 cggctcggcg agcgcttccg gtatccgttc cccgcctggg acgtgctgga gcaggttcag
     4261 cgaaagcggc accagctcga gcgtgccgtc gagctcggcg tcccgacgcc gcggacggcg
     4321 agcgggccga cgtccgacct cgggttcccc gtgctcgtca agccgtccga tccggtcgag
     4381 ttccgccgtg tgttcaagcg ccagtcgttt cgctgcgaga cgcgcgcgga gctcgacgag
     4441 gcgtgggagc gagcccgccc gtacgacccg ctcgtgcagg agctcatccc aggcggtgac
     4501 gaggagctct acacgctcgg cagctacctc tcggccgacg gcgaggcgct cgggctcttc
     4561 tgcggccgca agctccggca gacgccggcg ggggtcggga cggcccggat cggcgaggcg
     4621 gtctgggtgg acgaggtcgt cgagcagggg ctcgcgctcg tccgcggcct cggcgcgcac
     4681 gggatcgcgc agacggagtt caagcgcgac ccgcgcgacg gcgtcttcaa gctcatggag
     4741 gtgaacccac ggctctggca gtggcacggg ctcgccgccg cctgcggcgt cgacctgccg
     4801 cggatcgcct actgggacct gctcggcgcc cgcctgccgc cggcgcgctc gaacgggacg
     4861 cggaagcgat gggcgatcac gcacgtcgc
//
LOCUS       DASTFC010000037         8381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_973011,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000037 DASTFC010000000
VERSION     DASTFC010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973011"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFO88_01080"
     CDS             complement(<1..402)
                     /locus_tag="VFO88_01080"
                     /inference="COORDINATES: protein motif:HMM:NF044766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544147.1"
                     /translation="MESVDSAGRRDALVERLFASTLGAWDLLGVYLGDRLGLYRALSD
                     RGPLTSAELAEATGTHERYAREWAEQQATAGILEVDGEGRFLLPAGHDEALLDEGSLN
                     YIAPIAKLVVACTEPLPQLLDAFRSGEGVPYA"
     gene            complement(449..3172)
                     /locus_tag="VFO88_01085"
     CDS             complement(449..3172)
                     /locus_tag="VFO88_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9544148.1"
                     /translation="MPQTAPIGRETELALLAAAARAAGAGRGEIVFLDGPTGSGKSFL
                     LKSFAESLAEAERPDVVSVLCYETSAGNPLGPFGEALRALTSKERRGEKAKRALEVVG
                     QVAPPIVELIPVIGKLAALGVKTAADVGVWALGGSHEAQQTELAVDVALALRGIAGET
                     PIVVVLDDAQWIDAASTEVVARLAQAPEEHPLLLVVAYDGDLVPDTHPLARARAATAG
                     RSGVRTLELGALDVERVASFLADRYGSVPDEKLAAWLHDRTDGSPLFLSRFLDTLESQ
                     GVLSRTNGGWALDGTIAGGPGDWRLGGALAGAETPGTLLELLRPRVADLTDDERSLLE
                     TGAVQGRRFLSTVIVKLLDAEEDAILNRLAQIAERQRLIASEAIEDWWSDRSALYAFD
                     PGVLQEILYSRYAKSPYEKRRRHKAVAEALEALVADDDPPPRHALLEIARHYAEAGAP
                     LEAAARLVHVAESTFAEGADEETAEHAERAVALLRGAGPKATEAPETQRLLAQALLLF
                     LLGGEAGWSGTRGAGGDGDGAEERILALADEAERAAEASGDPRLRANARYAKARALTA
                     YRGLDEAIGAYGDALELARSAGDPLAEFAVLVDYGHHMDSVDLTRGWGLLQEAHGLLT
                     GGALDGHLGGSQHEAEQATLESSIGVAAFDLGRYGEALGLLAQSGDALSAARRRDEGA
                     WARAFLGQAYTAIGFFEAAEATLRDGTSLLAELPHPLGVRGYVGALLGRLYVEWEPPR
                     LEEARQALAPARVEVSASGYRPVLPLVETYWAELLLAEGTPDSLAQARGVLGAASSYG
                     WARSEIAICSLLARLALAEGRAADSVEPSTRAVTALAERGGAVPTVRSEEVLWTHARV
                     LEATGSAEAADYRARAAAVVRAKADSLTDPAQRESFLTRVRLSREILAAAG"
     gene            3221..3667
                     /locus_tag="VFO88_01090"
     CDS             3221..3667
                     /locus_tag="VFO88_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9544149.1"
                     /translation="MEVRLRDVEDADLPLFFAWQREPESVALSGVPARERDAFDEQWE
                     RIRRDPATVLRTVLADGEPVGNALAFEQSGRRVVGYRIARGHWGRGIATRALAAFVDE
                     LVERPLWATVAPGNAASVRVLEKCGFALEGEIEESGERVLLFRLAA"
     gene            complement(3724..3930)
                     /locus_tag="VFO88_01095"
     CDS             complement(3724..3930)
                     /locus_tag="VFO88_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET9544150.1"
                     /translation="MANGIVKWFSDEKGYGFITPDEGSKDLFVHHSNIAGEGFKSLSE
                     GARVQFEEREGAKGPEATDVAPIG"
     gene            complement(4024..4953)
                     /locus_tag="VFO88_01100"
     CDS             complement(4024..4953)
                     /locus_tag="VFO88_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544151.1"
                     /translation="MRDSPSGIGTASLARLGLAAFVLVWLFGPSSLRAVVPIWVPFLV
                     ALGLELQFFLTAWRAEPGTTGPDRRPQEIDRELYGYPPEPDEPGDEDDDAPDDYEEDE
                     EWEWEPEPEAQRSLPLRNLLLGVGVIVALAALAWVVDSRSGWGGLDDRTQAVAEERFS
                     AEASRIAEREVEIRCDESGERVGFVQHTDGVAEVGGRLAYLAPDRCHELYRLAFDGET
                     TGTTGRAIAVLAHEAWHLRGVRDESETQCYALQSGVEVGQRLGLSEDEARRLMRRELS
                     ANAGRGTSFEYFVGPECRDGGALDLDPGASDFP"
     gene            5049..5258
                     /locus_tag="VFO88_01105"
     CDS             5049..5258
                     /locus_tag="VFO88_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544152.1"
                     /translation="MEAEATTVAPGRTPEKARVIRWRTEELVRAGYSAFAADVLAVHT
                     EIDLHRAIELPRNGCPHDLALQILL"
     gene            complement(5274..5657)
                     /locus_tag="VFO88_01110"
     CDS             complement(5274..5657)
                     /locus_tag="VFO88_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544153.1"
                     /translation="MGATAREPGKVRSLHRVRVLLAAADPRFLRAATFLLEREGFAVV
                     GACKPDDAPGLARAERPHAVVVDGNASVAAAARTALAIEATGLPVHVLVIASGGGRRG
                     GPLTRVAECGSFVELTRELRRLYGS"
     gene            complement(5754..6374)
                     /locus_tag="VFO88_01115"
     CDS             complement(5754..6374)
                     /locus_tag="VFO88_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544154.1"
                     /translation="MKVLIADDHRLVLDSVRRTLERDEEIEVVGEATTGSQVLPFVRR
                     TSPDVLLLDLRMPQMDGLTCIEQVRKHCPNVKIVVLSAYTDAERIQAALDRGASGYVV
                     KSVNPDDLPGAVRHAVDGTFFGPVGAQGSEESDAVAAAGLTERELAVLKALARGLSNG
                     AIARESWISEQTVKFHLSNIYRKLGVANRTEAARFAYEHMLVESHA"
     gene            complement(6455..8101)
                     /locus_tag="VFO88_01120"
     CDS             complement(6455..8101)
                     /locus_tag="VFO88_01120"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HET9544155.1"
                     /translation="MTFTATVTADGGSPDGVGSVTFRNGATALCSAVALSGNTATCTA
                     SGLSAAASPHAVTAEYSGTAGGSPQFEPSTSATLSQAVHPKPVDGSFTAADKVYDGTA
                     DAEITGRALTGAIAGDDVSLSGGTASFDDENAGEDKPVSGTGFALAGADKDNYALGSV
                     AGTTATIEPRELAVAASGLDKVYDGTAAASTTFTDDRIPGDSLTIDYDASFADKHVGD
                     DKPVSVTGISLAGPDAGNYALTSTTAATTADITPRALVVTATGVDKVYDGTTAATVTL
                     ADDRVSGDELDVSYATAAFADAGAGTGKVIDVGGISVTGADAGNYTHNTSAVATADIA
                     PRPITVTAEPKSKPFGAPDPAFTYAVSGGPLVAGDGFSGSLTRVAGEAVGGYDILQGT
                     VSAGPNYAIAYVGATLTIGAWHAVGFHQPIGTGNSQFVPAPGLAPHVGTAVWNTAKGG
                     STIPLKFDLFTAAGGDERTSTADVKGFQAVRLTGCASGGGEEAVEFVTTGTTSLRYDG
                     TAGQFVQNWRSPSVSGEECYRVSVRFQDESAVYAFVKLRR"
     gene            8091..>8381
                     /locus_tag="VFO88_01125"
     CDS             8091..>8381
                     /locus_tag="VFO88_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544156.1"
                     /translation="MKVTEPPYVEGLELLASAVDVSRAGAATKFAVNVRFPEIALKQS
                     GLLVEEKVACAVHSSKSWPPELAGTSSVNEPVFCAKTFTPPDVGLAVAVPVAN"
BASE COUNT         1042 a         3114 c         3093 g         1132 t
ORIGIN      
        1 ggcgtacggg acgccctcgc cggaccggaa ggcgtcgagg agctgcggga gcggctccgt
       61 gcaggcgacg acgagcttcg cgatcggcgc gatgtagttg aggctcccct cgtcgaggag
      121 cgcctcgtcg tgcccggcgg ggaggaggaa gcggccctcg ccgtcgacct cgaggatccc
      181 ggcggtcgcc tgctgctccg cccattcgcg ggcgtagcgc tcgtgcgtgc cggtcgcctc
      241 ggcgagctcg gcgctcgtca gcgggccgcg gtctgagagc gcgcggtaga ggccgagccg
      301 gtcgccgagg tagacgccga ggaggtccca ggccccgagc gtggacgcga agagccgctc
      361 gacgagcgca tccctccgcc cggcgctgtc gacgctctcc atggcctctc cggtctccac
      421 tccgcgctcg cgcgcgagta tcccaggttc agccggccgc cgctaggatc tcgcgcgaga
      481 ggcgcacgcg ggtgaggaac gactcgcgct gggccggatc cgtgagcgag tccgccttcg
      541 cgcgcacgac cgccgccgcc cgcgcgcggt agtcggccgc ctccgcggag ccggtcgcct
      601 cgaggacgcg cgcgtgcgtc cagaggacct cctcgctccg gaccgtcggc acggcgccgc
      661 cgcgctcggc gagcgccgtg accgccctcg tgctcggctc gaccgagtcg gccgcgcgcc
      721 cctcggcgag cgcgaggcgg gccaggaggg agcagatcgc gatctcgctc cgcgcccacc
      781 cgtaggacga cgcggcgccg agcacgccgc gcgcctgggc gagcgagtcc ggcgtcccct
      841 cggcgagcag gagctcggcc cagtacgtct cgacgagcgg gaggaccggc cggtagcccg
      901 acgcggacac ctccacgcgc gccggcgcga gcgcctggcg cgcctcctcc aggcgcggcg
      961 gctcccactc gacgtacagc cgcccgagga gcgcgccgac gtagccgcga accccgagcg
     1021 gatgcggcag ctcggcgagg agcgaggtcc cgtcccggag cgtggcctcg gccgcctcga
     1081 agaagccgat cgccgtgtag gcctggccga ggaacgcgcg cgcccaggcg ccctcgtccc
     1141 gtcggcgcgc ggcgctcagc gcgtcgccgc tctgcgcgag cagtccgagc gcctcgccgt
     1201 agcggccgag gtcgaacgcc gcgaccccga tcgaggactc gagcgtcgcc tgctccgcct
     1261 cgtgctgcga gccgccgagg tgcccgtcga gcgctccgcc ggtgaggagc ccgtgcgcct
     1321 cctggagaag cccccagccc cgtgtgaggt cgacgctgtc catgtggtgg ccgtagtcga
     1381 cgagcacggc gaactctgcg agtggatccc cggcggagcg ggcgagctcg agcgcgtcgc
     1441 cataggcgcc gatcgcctcg tccagcccgc ggtaggccgt gagggcgcgc gccttcgcgt
     1501 agcgggcgtt cgcgcgcagc cgcgggtcac cggacgcctc cgcggcgcgc tccgcctcgt
     1561 ctgcgagcgc caggatccgc tcttccgccc cgtcgccgtc gcccccggcg ccgcgcgtcc
     1621 cgctccagcc cgcctcgccg ccgagcagga agagcaggag cgcctgcgcg aggagccgct
     1681 gcgtctccgg cgcctccgtc gccttcgggc cggcgccgcg gaggagcgca accgcccgct
     1741 cggcgtgctc ggccgtctct tcgtcggcgc cctccgcgaa cgtcgactcg gcgacgtgga
     1801 cgagccgcgc cgccgcctcg agcggcgcgc ccgcctccgc gtagtggcgc gcgatctcga
     1861 gcagggcgtg gcgcggcggc gggtcgtcgt cggccacgag cgcctcgagc gcctcggcga
     1921 ccgccttgtg ccgccgcctc ttctcgtacg ggctcttcgc gtagcggcta tagaggatct
     1981 cctggaggac gccggggtcg aaggcgtaga gcgccgagcg gtcgctccac cagtcctcga
     2041 tcgcctcgga ggcgatgagc cgctgccgct cggcgatctg cgccagccgg ttgaggatcg
     2101 cgtcctcctc cgcgtcgagg agcttcacga tcacggtcga gaggaaccgc cggccctgta
     2161 cggcgccggt ctcgagcagc gagcgctcgt cgtccgtcag gtcggcgacg cgggggcgga
     2221 ggagctcgag cagcgtgccc ggcgtctccg cgccggcgag cgcgccgccg aggcgccagt
     2281 cgcccggccc gcccgcgatc gtcccgtcga gcgcccaccc gccgttcgtc cggctgagga
     2341 cgccctgcga ctcgagcgtg tcgaggaagc ggctgaggaa gagcgggctc ccgtccgtgc
     2401 ggtcgtggag ccacgcggcg agcttctcgt ccggcaccga gccgtaccgg tcggcgagga
     2461 acgacgcgac tctctcgacg tcgagcgcgc cgagctcgag cgtgcggacc ccggagcggc
     2521 ccgccgtcgc cgcccgcgcg cgtgcgagcg gatgcgtgtc gggcacgagg tccccgtcgt
     2581 aggcgacgac gagcaggagc gggtgctcct ccggcgcctg tgcgagcctg gcgaccacct
     2641 cggtcgacgc ggcgtcgatc cactgcgcgt cgtcgaggac gacgacgatc ggcgtctcgc
     2701 cggcgattcc acgaagcgca agcgcgacgt cgacggcgag ctccgtctgc tgcgcctcgt
     2761 ggctcccgcc gagcgcccag acgccgacgt cggcggccgt cttcacgccg agcgcggcga
     2821 gcttgccgat caccgggatg agctccacga tcggcggcgc gacctggccg acgacctcga
     2881 gcgcgcgctt cgccttctcg ccgcgccgct ccttgctcgt gagcgcgcgc agcgcctccc
     2941 cgaacgggcc gagcgggttg cccgcgctcg tctcgtagca gaggacggac acgacgtccg
     3001 gccgctcggc ctcggcgagg ctctcggcga acgatttgag gaggaacgac ttgccgctcc
     3061 ccgtcggccc gtcgaggaag acgatctcgc ctcgcccggc gccggcagcg cgcgcggcgg
     3121 cggcgagcaa tgcgagctcc gtctcacggc cgatcggagc cgtctgcggc atggagaagg
     3181 tctagcgccg ccgggccgtg cgtgcaagag tcgccccgcg atggaggtcc ggctgcgcga
     3241 cgtcgaggac gcggatctcc cgctcttctt cgcgtggcag cgcgagcccg agtcggtcgc
     3301 gctctcgggc gtgcccgcgc gcgagcggga tgcattcgac gagcagtggg agcgcatccg
     3361 gcgcgacccg gcgactgtcc tgcgaaccgt gctggcggac ggcgagccgg tcgggaacgc
     3421 gctcgcgttc gagcagtccg ggcgccgggt ggtcggctac cggatcgccc gcgggcactg
     3481 gggccgcggg atcgcgacgc gcgcgctcgc ggcgttcgtc gacgagctcg tggagcggcc
     3541 gctctgggcg accgtagcgc cgggaaacgc cgcgtcggtc cgcgtgctcg agaagtgcgg
     3601 gttcgccctg gagggcgaga tcgaggagag cggcgagcgc gtgctgctct tcaggctcgc
     3661 ggcgtaggcg ggccggaggt cgcccgtgac cgcgctgggc ggccacgggc tcctcggaac
     3721 gggctagccg atcggggcga cgtcggtcgc ctccgggccc ttagcgccct cgcgctcctc
     3781 gaactggacg cgggcaccct cggagagcga cttgaagccc tcaccggcga tgttggagtg
     3841 gtggacgaag agatccttcg atccctcgtc gggggtgatg aagccgtacc ccttctcgtc
     3901 actgaaccac ttcacgattc cgttagccat gaaaacacct cccttgctta ctcacgaacg
     3961 tgttaggaag gcgcttggcg caaaccggac tcgctctaga ttgccttcag cgtagcagcg
     4021 atctcacggg aagtcgctcg cgccggggtc gagatcgagc gcgccgccgt cgcggcactc
     4081 ggggccgacg aagtactcga agctcgtccc gcggccggcg ttcgccgaga gctcgcgccg
     4141 catgagccgc cgcgcctcgt cctccgagag cccgagccgc tgccccacct cgacgccgga
     4201 ctggagcgcg tagcactgcg tctcgctctc gtcgcggacg ccgcgcaggt gccaggcctc
     4261 gtgcgcgagg accgcgatcg cccggccggt cgtgccggtg gtctcgccgt cgaacgcgag
     4321 gcggtagagc tcgtggcagc ggtcgggcgc gaggtacgcg agtcgcccgc cgacctccgc
     4381 gacgccgtcc gtgtgctgga cgaacccgac gcgctcgccc gactcgtcgc agcggatctc
     4441 gacctcgcgc tcggcgatgc gggacgcctc ggcggagaag cgctcctcgg cgaccgcctg
     4501 cgtccggtcg tcgagcccgc cccagccgct gcgggagtcc acgacccacg cgagcgcggc
     4561 gagggcgacg atcacgccga ccccgagcag gaggttgcga agcggcagcg accgctgcgc
     4621 ctcgggctcc ggctcccact cccattcctc gtcctcctcg tagtcgtcgg gcgcgtcgtc
     4681 gtcctcgtcc ccgggctcat ccggctcggg cgggtagccg tagagctcgc ggtcgatctc
     4741 ctgcgggcgc cggtcgggcc cggtcgtccc gggctccgcg cgccaggccg tgaggaaaaa
     4801 ctgcagctcg aggccgagcg ccacgaggaa cggcacccag atcggcacca ccgcccgaag
     4861 cgaggacggc ccgaagagcc agacgaggac gaaggcggcg aggccgaggc gggcgaggct
     4921 cgcggtgccg atgccggagg gggagtcgcg cacgcgggga ccgtagccgc gggaacccac
     4981 ccgaacgtcc ggctccaccc accgatccgt cagtttcggg tccggccggc tcggggcacg
     5041 ctgaccccat ggaagccgag gccacgaccg tcgcaccggg gcgcacgccg gagaaggcgc
     5101 gcgtcatccg ctggcgcacc gaggagctcg tccgcgccgg gtacagcgcc ttcgcggccg
     5161 acgtcctcgc cgtgcacacg gagatcgacc tccaccgggc gatcgagctg ccgcgcaacg
     5221 gctgcccgca cgacctcgcg ctccagatcc tcctctgaga gccgcgagct gcgctacgag
     5281 ccgtagaggc ggcgcagctc gcgcgtcagc tcgacgaacg agccgcactc ggcgacacgg
     5341 gtgagcggcc ctccgcgccg gccgccgccc gaggcgatca cgagcacgtg cacgggaagg
     5401 ccggtcgcct cgatcgcgag cgccgtccgg gcggcggcgg cgacggacgc gttcccgtcg
     5461 acgacgacgg cgtgcgggcg ctcggcgcgg gcgaggccgg gcgcgtcgtc cggcttgcac
     5521 gccccgacga cggcgaagcc ctcacgctcg agcaggaacg tcgccgcgcg gaggaagcgc
     5581 gggtcggcgg ccgcgagcag gacgcgcacg cggtgcagcg acctgacctt gcccggctct
     5641 cgcgcggtgg cacccatcgg atgcccggag tctcgtccct cgccgcctcg tgcgggccgc
     5701 cggtccggac gggccgtgcc acccgaacgg ccggttcacc ggcgcggcgt cgatcaggcg
     5761 tggctctcga cgagcatgtg ctcgtaggcg aagcgcgcgg cctccgtccg gttggccacc
     5821 ccgagcttcc gatagatgtt cgagaggtgg aacttgacgg tctgctcgct gatccaggac
     5881 tccctcgcga tcgcgccgtt cgagaggccg cgggcgagcg ccttcagcac ggcgagctcc
     5941 cgctcggtga ggcccgccgc cgcgaccgcg tcgctctcct cggacccctg cgcgccgacg
     6001 ggcccgaaga acgtcccgtc gaccgcgtgg cggacggcgc cgggcaggtc gtccgggttc
     6061 acgctcttga ccacgtagcc gctcgccccg cggtcgagag cggcctggat ccgctcggcg
     6121 tcggtgtacg cggagaggac gacgatcttc acgttcgggc agtgcttgcg gacctgctcg
     6181 atgcacgtga gaccgtccat ctgaggcatg cgcagatcga ggaggaggac atcgggggac
     6241 gtccggcgga cgaacggaag cacctggctg ccggtcgtcg cctccccgac cacctcgatc
     6301 tcctcgtccc gctcgagggt cctccggacg ctgtcgagca cgagccggtg gtcgtccgca
     6361 atcagcacct tcatcgccgc atcgctcctt tcgccttgta ggaggggggc cagccgccgc
     6421 cgggccggcc ccccacgcgc cgccctccgg ccgctcagcg gcggagcttc acgaaggcgt
     6481 acaccgccga ctcgtcctgg aagcggacgc tcacgcggta gcactcctcc ccgctcacgg
     6541 agggcgatct ccagttctgg acgaactggc cggccgtgcc gtcgtagcgg aggctcgtgg
     6601 tgccggtcgt gacgaactcg accgcctcct cgcctccgcc gctcgcgcag ccggtcagcc
     6661 tgacggcctg gaaccccttg acgtccgccg tgctcgtccg ctcgtcgccg cccgcggccg
     6721 tgaagaggtc gaacttgagc gggatcgtcg agccgccctt cgccgtgttc cagacggcgg
     6781 tgccgacgtg gggcgcgagg cccggcgccg gcacgaactg cgagttcccg gtcccgatcg
     6841 gctggtggaa gccgaccgcg tgccacgcgc cgatcgtgag cgtggcgccg acgtacgcga
     6901 tcgcgtagtt cgggccggcc gagacggtgc cctggaggat gtcgtaaccg ccgacggcct
     6961 cgcccgcgac gcgcgtcagg ctgccggaga acccgtcccc ggcgacgagc ggcccgccgc
     7021 tcacggcgta cgtgaaggcc gggtcgggcg cgccgaacgg cttgctcttc ggctcggcgg
     7081 tcaccgtgat cggccgcggc gcgatgtccg cggtcgcgac ggccgacgtg ttgtgcgtgt
     7141 agttgcccgc gtcggcgccg gtgaccgaga tcccgccgac gtcgatcacc ttgcccgtcc
     7201 cggcgccggc gtccgcgaac gcggcggtcg cgtagctcac gtcgagctcg tcaccgctca
     7261 cccggtcgtc cgccagggtc acggtcgcgg cggtggtgcc gtcgtacacc ttgtccacgc
     7321 cggtcgccgt cacgacgagg gcgcgcggcg tgatgtcggc ggtcgtcgcg gccgtcgtgc
     7381 tcgtgagcgc gtagttgccg gcgtccgggc ccgcgagcga gatcccggtc acgctcaccg
     7441 gcttgtcgtc gcccacgtgc ttgtcggcga acgaggcgtc gtagtcgatc gtgagcgagt
     7501 cgccggggat ccggtcgtcg gtgaacgtcg tgctcgccgc cgccgtgccg tcgtagacct
     7561 tgtccaggcc ggacgcggcc acggcgagct cgcgaggctc gatcgtcgcc gtcgtgcccg
     7621 cgacggagcc gagggcgtag ttgtccttgt cggcgccggc gagcgcgaag cccgtgccgc
     7681 tgaccggctt gtcctccccg gcgttctcgt catcgaagct cgcggtgccg ccggagaggc
     7741 tgacgtcgtc gccggcgatc gcgcccgtga gcgcgcgccc ggtgatctcc gcgtcggcgg
     7801 tgccgtcgta gaccttgtcg gcggcggtga agctcccgtc gacgggcttc gggtggacgg
     7861 cctgcgacag ggtcgcgctc gtgctgggct cgaactgcgg cgagccgccc gccgtgccgc
     7921 tgtactcggc cgtgaccgcg tgcgggctcg cggcggcgga caggccggac gccgtgcacg
     7981 tcgcggtgtt gccgctcagc gcgacggcgg agcagagcgc ggtcgcaccg ttccggaatg
     8041 tcaccgaccc caccccgtcc gggctcccgc cgtcggcggt gaccgtcgcc gtgaaggtca
     8101 cggagccacc gtacgtcgag gggttggagc tgctcgcgag cgccgtcgac gtctcccggg
     8161 cgggcgccgc cacgaagttc gccgtgaacg tccggttccc cgagatcgcg ttgaagcaga
     8221 gcgggttgct cgtcgaggag aaggtcgcct gcgccgtcca ctcgtcgaag tcctggccgc
     8281 ccgagctcgc ggggacctcg agcgtgaacg agccggtgtt ctgcgcgaag acgttcacgc
     8341 cgcccgacgt cgggctcgcc gtcgccgtgc cggtggcgaa c
//
LOCUS       DASTFC010000038         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_544531,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000038 DASTFC010000000
VERSION     DASTFC010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544531"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFO88_01130"
     CDS             complement(<1..826)
                     /locus_tag="VFO88_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9544157.1"
                     /translation="MQDVLSVSNQVAAELATVDDSVLSRLRDRLGATVLLRGNRLTLE
                     GDEANVAEARAVIDELVELVEGGHAIGAGTVDAVLAALDESRDLRDVFEDVVWRHRSK
                     KITPKTVNQKRYVDAIRKGTVTFGIGPAGTGKTYLAMALAVAALSERQVNRIILTRPA
                     VEAGERLGFLPGDMLAKVDPYLRPLYDALYDMIDSYRVNSYMERGTIEFAPLAFMRGR
                     TLNHAFVILDEAQNTTPEQMKMFLTRIGFGAKAVVNGDVTQIDLARGQKSGLIEARR"
     gene            complement(997..1440)
                     /locus_tag="VFO88_01135"
     CDS             complement(997..1440)
                     /locus_tag="VFO88_01135"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET9544158.1"
                     /translation="MTLISELEDEIKDALRERDTERLDTLRLVLAELRAAEKELLRPL
                     DESEELQVLQRERKRRLEAAEAFRAADREEQAEKEERELDVIEEFMPDPIDEDELEEI
                     VDDAIAETGATSLRDLGRVMADVMPQVAGRADGSTVSRLVREKLA"
     gene            complement(1437..1769)
                     /locus_tag="VFO88_01140"
     CDS             complement(1437..1769)
                     /locus_tag="VFO88_01140"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9544159.1"
                     /translation="MSQPDCLFCKLVAEGDHVRAADGFVAIRDIAPKAPTHLLVLPER
                     HVDSFREIAEFPPDESKGMLAFIADTAREAGLEDYRVIVNVGPGAGQTVFHLHWHVLG
                     GNGLGGFE"
     gene            complement(1756..>2717)
                     /locus_tag="VFO88_01145"
     CDS             complement(1756..>2717)
                     /locus_tag="VFO88_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9544160.1"
                     /translation="GDAFAGLPANVRVVPLPSERTAAFVAGDVGAVGCVRADVGLDRV
                     RACVKVQDGCSFSCNFCVIPQVRGASRSRPAAAVLAELRKRVTQGHREIVLTGINLGC
                     YRDRAAGFDLPRLVREAGAIPGLARLRLSSVEVNHLTDDLVAAMRETPTVSRHLHVPL
                     QSGDDGVLRAMGRRYTVATFLRRVARAGGFNLTSDVIVGFPAEDDAAFERTLATVRAA
                     GITKVHVFPYSPRPGTRTAADDPVPAETKRERSERLRAASDAACRRRWRERIGSHDDV
                     LVDRPGRGYADDYTPWLVEGPVGEVVRARAERVTSEGIVGVAA"
BASE COUNT          375 a          966 c          979 g          397 t
ORIGIN      
        1 tgcgccgcgc ctcgatgagg ccgctcttct gcccgcgcgc gaggtcgatc tgcgtgacgt
       61 cgccgttcac caccgccttg gcgccgaagc cgatgcgcgt gaggaacatc ttcatctgct
      121 cgggcgtcgt gttctgcgcc tcgtccagga tgacgaaggc gtggttgagc gtgcggccgc
      181 gcatgaacgc gagcggggcg aactcgatcg tgccgcgctc catgtacgag ttgacgcggt
      241 aggagtcgat catgtcgtag agagcgtcgt agagcgggcg caggtacggg tcgaccttcg
      301 cgagcatgtc gccggggagg aacccgagcc gttcgccggc ctcgaccgcc gggcgcgtga
      361 ggatgatccg gttcacctgg cgctcggaga gcgccgcgac ggcaagggcc atggcgaggt
      421 aggtcttgcc cgtgccggcc ggcccgatcc cgaacgtgac cgtgcccttg cggatcgcgt
      481 ccacgtagcg cttctggttc accgtcttcg gggtgatctt cttcgagcgg tgccgccaga
      541 cgacgtcctc gaagacgtcc ctgaggtcgc ggctctcgtc gagcgcggcg agcacggcgt
      601 cgacggtgcc ggccccgatc gcgtggccgc cctccacgag ctcgacgagc tcgtcgatca
      661 cggcgcgcgc ctcggcgacg ttcgcctcgt cgccctcgag cgtcagccgg ttgccgcgca
      721 ggagcacggt cgcgccgagc cggtcgcgca gccggctgag cacggagtcg tcgacggttg
      781 cgagctcggc ggcgacctga ttggagaccg agagaacgtc ctgcaagctc tcctcagtgt
      841 agatgcggct tccgggggca tcgggccgcg ccctgggtca gacgcgcgga gtgatttccc
      901 cggggacccc ctctggttac ccctcaccgg tcaggctaac ggcgagaagc gcaccgttcg
      961 tgccacgagt gtgacgaaag cgtgtcggcg accgacctac gcgagcttct cgcggacgag
     1021 ccggctcacg gtcgagccgt cggcgcggcc ggcgacctga ggcatgacgt cggccatcac
     1081 gcggccgagg tcgcggaggg acgtcgcccc cgtctcggcg atcgcgtcgt cgacgatctc
     1141 ctcgagctcg tcctcgtcga tcgggtcggg catgaactcc tcgatcacgt cgagctcgcg
     1201 ttcctccttc tcggcctgct cctcccggtc ggccgcacgg aacgcctcgg cggcctcgag
     1261 ccgccgcttg cgctcacgct ggagcacctg gagctcctcg ctctcgtcga gcgggcgaag
     1321 cagctccttc tcggccgcgc gcagctccgc gagcacgagc cggagcgtgt cgagccgctc
     1381 cgtgtcgcgc tcgcggagag cgtccttgat ctcgtcttcg agctcggaga tgagggtcat
     1441 tcgaagcctc ccaggccgtt ccccccgaga acgtgccagt gcaggtggaa caccgtctgg
     1501 ccggcgcccg gcccgacgtt cacgatcacg cggtagtcct cgagcccggc ctcgcgggcc
     1561 gtgtccgcga tgaacgcgag catccccttc gactcgtccg gcgggaactc ggcgatctcc
     1621 cggaaggagt cgacgtggcg ctccgggagg acgagaaggt gcgtcggcgc cttcggagcg
     1681 atgtcacgga tggccacgaa gccgtcggcc gcgcgaacgt ggtcgccctc ggcgacaagc
     1741 ttgcagaaca ggcagtcagg ctgcgacacc gacgatcccc tcactggtca cgcgctcggc
     1801 ccgcgcccgg acgacctccc cgaccgggcc ctcgacgagc cagggagtgt agtcgtccgc
     1861 gtagccccgg cccgggcggt cgacgaggac gtcgtcgtgc gagccgatgc gctctcgcca
     1921 gcgccgccgg cacgccgcgt cggacgccgc ccggaggcgc tcgctgcgct cccgcttcgt
     1981 ctcggcgggg acagggtcat cggcggcggt gcgcgtgccc gggcgcggcg agtacgggaa
     2041 gacgtgcacc ttcgtgatcc ccgcggcccg gacggtcgcg agcgtgcgct cgaaggccgc
     2101 gtcgtcctcg gccggaaagc cgacgatgac gtcgctcgtc aagttgaagc cgcccgcgcg
     2161 ggcgacccgg cggaggaacg tggcgaccgt gtaccggcgg cccatcgcgc gcagcacgcc
     2221 gtcgtcgccg gactggagcg gcacgtgcag gtgcctggag acggtgggcg tctcgcgcat
     2281 cgcggcgacg aggtcgtccg tcaggtggtt cacctcgacg gagctcagcc gcaggcgcgc
     2341 gaggccgggg atcgcgcccg cttcccgtac gagccgcggc aggtcgaacc cggccgcgcg
     2401 atcgcggtag cagccgagat tgatcccggt aaggacgatc tcgcgatggc cctgcgtcac
     2461 gcgcttgcgg agctcggcga gaaccgcggc ggccggccgg ctccgcgacg cgccgcgcac
     2521 ctgcgggatg acgcagaagt tgcacgagaa cgaacagccg tcctgcacct tcacgcacgc
     2581 gcgcacgcga tcgaggccga cgtcggcccg cacgcagccg accgcgccga cgtcgcccgc
     2641 gacgaacgcc gcggtgcgct cgctcgggag cgggacgacg cggacgttcg ccgggaggcc
     2701 ggcgaaggcg tcgcccg
//
LOCUS       DASTFC010000039         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1236756,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000039 DASTFC010000000
VERSION     DASTFC010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236756"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFO88_01150"
     CDS             <1..189
                     /locus_tag="VFO88_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544161.1"
                     /translation="KALGCGVHFTWREIEIAGRPKPGVNLSGWTKAWSERVRAGSIDL
                     SMTHSKELAAAICVVTDP"
     gene            186..1679
                     /locus_tag="VFO88_01155"
     CDS             186..1679
                     /locus_tag="VFO88_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9544162.1"
                     /translation="MSAFEPLFTAEEMRAAEAAYPGPTEELMERAGAAVADAVAASLP
                     DARRVAVWCGTGANGGDGFVVARLLHTAGREVEAVLVGDEGRVAGDAATMLARAREAG
                     VPVRPFADVAGGRADVVVDALFGTGFSGAPRREAAAAIEALSGLGGAVVAVDVPSGVD
                     ASTGRVGGVAVEAARTVTFHGAKVGLAVGPGRFHAGRVEVADIGLAAGGETRARRVTA
                     DALELVPRRGPRDNKYSAGFVCVVGGSAGLTGAPFLASEAAMRAGAGYVRACVPASLA
                     PGFAGRFVEVTTTACPDDGEGRLTEAAADVVLEAAAKADCVALGPGLGRSDGTRALVR
                     ALLDRLEIPVVLDGDGLWALAGHLDWVFSRDAPTVLTPHSGELAGLLGRDAAWVDANR
                     LDAAGGAADDVGAVVCLKGADTLVAAPGRGLLVCDLGSPGLASAGTGDVLTGIVAAFL
                     AKGMAAPTAAAVGAAAGGVAAAEAARRHGEAGLIARDVVEALSPVLS"
     gene            1927..>2627
                     /locus_tag="VFO88_01160"
     CDS             1927..>2627
                     /locus_tag="VFO88_01160"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9544163.1"
                     /translation="MEPETETTRGRSEVTIDLGALRRNVRRLREVAAPAELWAVVKAD
                     AYGHGANDAARTALAEGAAALCVATAREGVALRARIPRATRILVMGPLDAGDEALAQE
                     HGLEVAVSRLPLPDGLAVHLKVDTGMGRYGLTAAEALSVPPDRVAGVMSHLATADEPD
                     ETFAREQLEAFAALAARFPDATAHLANSAATLRFPEARHDAVRCGVALYGLDPFGRDP
                     GDQALAPVLSWRSRVA"
BASE COUNT          278 a          948 c         1077 g          324 t
ORIGIN      
        1 aaggcgctcg gctgcggcgt ccacttcacg tggcgcgaga tcgagatcgc cgggcggccg
       61 aagccgggcg tgaacctctc cggctggacg aaggcgtgga gcgagcgggt tcgtgccggc
      121 tcgatcgacc tctcgatgac gcactcgaag gagctcgccg ccgcgatctg cgtcgtgacc
      181 gacccgtgag cgcgttcgag ccgctcttca ccgcggagga gatgcgggcg gccgaggcgg
      241 cctaccccgg cccgaccgag gagctcatgg aacgggcggg cgcggcggtg gccgacgcgg
      301 tggccgcgtc gttgccggac gcccggcgtg tcgccgtctg gtgcgggacc ggcgcgaacg
      361 gcggggacgg cttcgtcgtg gcgcggctgc tccacacggc cgggcgcgag gtggaggcgg
      421 tgctcgtcgg ggacgagggg agggtcgcgg gcgacgccgc gacgatgctc gcgcgggcgc
      481 gcgaggccgg cgtccccgtg cggccgttcg cggatgtggc cgggggccgg gccgacgtcg
      541 tcgtggacgc gctcttcggg acggggttct cgggcgcgcc ccggcgggag gccgccgcgg
      601 cgatcgaggc gctctcgggg ctcggcggcg ccgttgtcgc ggtggacgtg ccctcggggg
      661 tcgacgcatc gaccggccgt gtgggcggcg tcgcggtcga ggcggcacgc acggtcacgt
      721 tccacggcgc gaaggtcggt ctcgcggtcg ggccgggacg gttccacgcc gggcgcgtgg
      781 aggtggcgga catcgggctc gcggcgggcg gcgagacgcg ggcgcggcgg gtgacggcgg
      841 acgcgctcga gctcgtgccc cggcgcgggc cgcgcgacaa caagtactcc gcggggttcg
      901 tgtgcgtcgt cggcgggtcg gcggggctga ccggcgcgcc gttcctcgcg tctgaggcgg
      961 cgatgcgcgc gggggccggc tacgtgcgcg cgtgcgtccc ggcgtcgctc gccccggggt
     1021 tcgccgggcg gttcgtcgag gtgacgacga ccgcgtgccc ggacgacgga gaggggcgcc
     1081 tgacggaggc ggcggccgac gtcgtgctcg aggcggcggc gaaggcggac tgcgtcgcgc
     1141 tcggcccggg gctcgggcgg agcgacggga cccgggcgct cgtgcgggcg ctcctcgacc
     1201 ggctggagat ccccgtcgtg ctcgacggcg acgggctctg ggcgctcgcc gggcacctgg
     1261 actgggtctt ctcccgggac gcgccgaccg tgctcacgcc gcactccggc gagctggccg
     1321 ggctgctcgg gcgcgacgcg gcctgggtgg acgcgaaccg gctcgacgcg gccggcgggg
     1381 cggcggacga cgtcggcgcc gtggtgtgcc tgaagggggc ggacaccctt gtcgccgcac
     1441 ccggccgcgg gctgctcgtc tgcgacctcg gcagcccggg gctcgcgagc gccgggacgg
     1501 gcgacgtgct gaccgggatc gtggccgcgt tcctcgccaa gggcatggct gcgcccaccg
     1561 ccgctgcggt cggtgccgcc gccggcggag ttgccgccgc cgaggccgcg cgccgccacg
     1621 gcgaggcggg cctcatcgcc cgcgacgtcg tcgaggccct ctcgcccgtc ctctcgtagt
     1681 taatcgcgcg cgcctagcgg gtcggacgcc gtaagggggt cagaccccgc acaaaagcgc
     1741 atgagaatgt gtgccgactt cgtaacgtcc ctgctcggag cgctgttctc gaccgtctga
     1801 acctgtcacg ggcgtgtgac gggcccgagc tcacggggtc agaccccgtc gcggaagccg
     1861 cacgcgttcg tcccggaacg gcgacagggc cacgcggccg tcacgcgggg cggggacaat
     1921 cccggcgtgg agcctgagac cgagacgacc cgcggccgtt ccgaggtcac gatcgacctc
     1981 ggcgcgctgc ggcgcaacgt ccgccggctg cgcgaggtcg cggccccggc cgagctctgg
     2041 gcggtcgtca aggcggacgc ctacggtcat ggcgcgaacg acgccgcccg gacggcgctc
     2101 gccgaggggg cggccgcgct ctgcgtcgcc accgcgcgcg agggcgtggc gctgcgcgcg
     2161 cggatcccgc gcgcgacgcg gatcctcgtc atgggcccgc tcgacgccgg cgacgaggcg
     2221 ctggcgcagg agcacgggct cgaggtcgcc gtctcccggc tcccgcttcc ggacggcctt
     2281 gccgtccacc tcaaggtcga caccggcatg ggccgctacg ggctcacggc ggcggaggcg
     2341 ctgagcgtcc caccggaccg ggtcgcgggc gtgatgagcc acctcgcgac ggccgacgag
     2401 cccgacgaga ccttcgcccg cgagcagctc gaggcgttcg cggccctcgc cgcgcgcttc
     2461 cccgacgcga ccgcgcacct cgcgaacagc gcggccacgc tccgctttcc cgaggcgcgc
     2521 cacgacgcgg tccggtgcgg cgtcgccctg tacgggctcg acccgttcgg ccgcgacccg
     2581 ggcgaccagg ccctcgcccc cgtcctctcg tggcggagcc gcgtggc
//
LOCUS       DASTFC010000040         6542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1533480,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000040 DASTFC010000000
VERSION     DASTFC010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533480"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFO88_01165"
     CDS             complement(<1..752)
                     /locus_tag="VFO88_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9544164.1"
                     /translation="MTDWPAVCRRCGEAVAAALAELPTRAERERVVGAGEGGDETTAV
                     DAVAEAAVVRVLEDGVGEGFTLVSEELGERRFGDGGDGLRVVCDPIDGSLNAKRGFPL
                     FALSLAVADGPAMEDVFFAYVLDYGTGEEWTATRGDGARLNGEPLTGPGPKGEVELCV
                     IEATRTDLLAEAVGAFVGFAYRTRVFGSLALSLCHLAAGRVDAACSLKPARSVDIAAA
                     QLLVRERGLAIDLPEDAPFGRARLDLVPRSRCV"
     gene            complement(749..1882)
                     /locus_tag="VFO88_01170"
     CDS             complement(749..1882)
                     /locus_tag="VFO88_01170"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET9544165.1"
                     /translation="MSLVKPFRALRYDPERAGPLESVVAPPHDVVRPGQREELHEASP
                     YNAIRLTRPDDPEEAGRLFHAWIEEGVLVREPEPAVWILEETYTGPDGAERTRRSLVA
                     RVRMSRYGEGEVFPHERTFRRQKQFRLELLRATRTKLSPVFFLHEGSTPAHPEGPPDL
                     EAAFEGVASRLWRVSEPAAVAAALALVRGPLIIADGHHRYETALRFHEENGTEETAYV
                     LGVLVAHDDPGLEIFPTHRLAGPPVPEPNGRFRLTELPGGAADGLARLGELPRDRPAF
                     VLLHRGGAALAETDPVADGPAGELDTAVLDELGLDDVRFTPSAEEAEQAVASGEAAAA
                     FLVRAPTVAQVEAVALAGETMPQKSTYFFPKLTSGLLFAPFDE"
     gene            1950..2831
                     /locus_tag="VFO88_01175"
     CDS             1950..2831
                     /locus_tag="VFO88_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9544166.1"
                     /translation="MSVALQHAVSPGARAARATIAELVEDRIVEGVFAVARKRRLSTR
                     AGRPYIAIELVDATGQIEGRVWSDVELLDRRFREGDAVRVLARVERFKERLQLDVRSL
                     EPVEADPASLVPQARRDLEELDGFLEFIAEELAHPGLRRVVDGLVGDSAFRERMRTLP
                     ATPGGHHGYSGGLLEHTVAVTTLCRDAADLHKRLRRDLLLAAALLHDAGRTLELDRGP
                     LARPTPEGRLLGHVHLGLRLIEERSAELEAEVRAELLHAVAVHHDPRAARTAEALVLY
                     HANQLDAVAATRPVDGE"
     gene            2860..3345
                     /locus_tag="VFO88_01180"
     CDS             2860..3345
                     /locus_tag="VFO88_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544167.1"
                     /translation="MADERLLRIYLNDHLALATAAVRLARRSAKSNAGSDLGRTLERI
                     AAELDDERHVFARAVEALGYRRNRAKEAAAVVGERLGLLKLNGHVVSPSPLSRVVELE
                     ALAALVGWNARAWRTLAGLPQLAERLAPLDLERLATEADERQAELDRFRPGAASTAFV
                     G"
     gene            3366..4580
                     /locus_tag="VFO88_01185"
     CDS             3366..4580
                     /locus_tag="VFO88_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET9544168.1"
                     /translation="MPVPQEQLDAYAELVVGVGVNLEPGQAFGVTAYVEHAPFVRAVA
                     RAAWAAGARDVDVRYVDEHLRKAAVQLAPEESLGVSPPWLLDRRESYGDRSAYLLVRG
                     HPDPRMFEDVDGARLAASRLHEEDAIWLRQVSERRVSWSLVSFPTEGWAATVFGEPDV
                     GRLWDAIARTVRLDEPDPVAAWAEHIRRLDARAAALNERRFDAIRFRGPGTDLLVGLH
                     AGSRWQSAGEETAWGRPHVPNLPTEEIFTTPDRRRTEGRVRSTRPLVCQGRIVRGLEI
                     ELRAGRVVDVRAEEGAEVVQAQVATDEGAAELGEIALVDGDSRVGREGLTFFDTLFDE
                     NASSHVAYGQGFGGCVDGAAELSPEEQWELGISQSSLHTDFMVGGPDVEIDGLTASGE
                     AVAIVRGDAWQL"
     gene            complement(4863..5801)
                     /locus_tag="VFO88_01190"
     CDS             complement(4863..5801)
                     /locus_tag="VFO88_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HET9544169.1"
                     /translation="MALQAGIVGLPNAGKTTLFNALTRAGAEITAYATVSDKANVGMA
                     PIADERLGEVAAVVSARKVTPAAVRVVDVPGSGRQLLGGLREVDALVAVLNGFRPDAD
                     PDADLETIELELLLADREHVERRLERVEKQAKSGEAALRAEADELRRLLAHLDGGGAL
                     RDYPGGLPEALEPLTTKPIVPVVNGPGGIDLKLEAELAELPEDEAAAFREGPSALGEI
                     VARLFAALDLISFFTAGDKETRAWTLRRGETALDAAAEIHTDIARGFIRCEVIRWRDL
                     VECGSAAEAAKRGLQRLEGKTYEVEDGDVLNIRFNV"
     gene            complement(5819..6391)
                     /locus_tag="VFO88_01195"
     CDS             complement(5819..6391)
                     /locus_tag="VFO88_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9544170.1"
                     /translation="MERRVRGDAEEAGPDVRRLVERAQAGDRAAIEELYRTHFDRIYG
                     YLRLSVGSQHDAEDLTNQTFIRMLESLDRFVWQKVPFSAWLFRIAHNLAIDHFRAGRR
                     WQPEGDVPERISEVSPSAEEAAFHTIDRERMLGLIRGLSRDQQQVLTLRYVFDFTNGE
                     AAAILGKTENAVKQLQHRAIGSLQQRLVHA"
BASE COUNT          813 a         2412 c         2463 g          854 t
ORIGIN      
        1 acgcagcggg agcgcgggac gaggtcgagc cgggcccgcc cgaagggcgc gtcctcgggc
       61 aggtcgatcg cgaggccgcg ctcccggacg aggagctgcg cggccgcgat gtcgacgctc
      121 cgcgcgggct tgagcgagca ggccgcgtcc acgcggccgg cggcgaggtg gcagagcgag
      181 agcgcgagcg agccgaagac gcgggtccgg taggcgaacc cgacgaacgc gccgaccgct
      241 tcggcgagca ggtccgtgcg agtcgcctcg atcacgcaca gctcgacctc gcccttcgga
      301 cccggcccgg tgagcggctc gccgttcagc cgcgcgccgt cgccgcgcgt cgccgtccac
      361 tcctcgcccg tgccgtagtc gaggacgtac gcgaagaaga cgtcctccat cgccggcccg
      421 tccgcgaccg cgagcgagag ggcgaagagc gggaagccgc gcttcgcgtt caggctcccg
      481 tcgatcgggt cgcagacgac gcgcaggccg tccccgccgt ccccgaaccg gcgctcgccg
      541 agctcttccg agacgagcgt gaagccctcg ccgaccccgt cttcgaggac gcgcacgacc
      601 gcggcctcgg cgacggcgtc gacggcggtc gtctcgtcgc cgccctctcc ggcgccgacc
      661 acccgctcgc gctcggcgcg ggtgggcagc tctgcaagcg ccgctgcgac cgcctcgccg
      721 cagcgccggc agaccgcggg ccagtcggtc attcgtcgaa cggcgcgaag aggaggccgc
      781 tcgtcagctt cggaaagaag tacgtcgact tctgcggcat cgtctcgccc gcgagcgcga
      841 cggcctcgac ctgcgccacc gtcggcgcgc gcacgaggaa ggcggccgcg gcctcgcccg
      901 acgcgacggc ctgctcggcc tcctcggcgc tcggcgtgaa ccggacgtcg tcgaggccga
      961 gctcgtcgag gacggccgtg tcgagctcgc ccgccgggcc gtcggctaca gggtcggtct
     1021 ccgcgagcgc cgcgccgccc cggtgcagga ggacgaacgc cggccggtcg cgcggcagct
     1081 ccccgaggcg cgcgagcccg tccgccgcgc cgccggggag ctcagtcagc cggaagcgcc
     1141 cgttcggctc cggtaccgga gggcccgcga gccggtgcgt ggggaagatc tcgagccccg
     1201 ggtcgtcgtg ggcgacgagg acgccgagga cgtaggcggt ctcctccgtc ccgttctcct
     1261 cgtggaagcg gagcgcggtc tcgtaccggt ggtgcccgtc cgcgatgatg agcggcccgc
     1321 gcacgagcgc gagggccgcg gcgaccgcgg ccggctcgga gacgcgccag aggcggctcg
     1381 cgacgccctc gaacgccgcc tcgaggtcgg gcgggccctc ggggtgcgcg ggcgtcgagc
     1441 cctcgtggag gaagaagacg ggcgagagct tcgtccgcgt cgcgcgcagg agctccaggc
     1501 ggaactgctt ctgccggcgg aacgtccgct cgtgcgggaa gacctcgccc tcgccgtagc
     1561 gcgacatccg cacgcgggcg acgaggctgc ggcgcgtccg ctcggcgccg tcggggcccg
     1621 tgtacgtctc ctcgaggatc cagacggccg gctcgggctc gcgcacgagc acgccttcct
     1681 cgatccacgc gtggaagagg cgtcctgcct cctcggggtc gtcgggccgc gtcaggcgga
     1741 tcgcgttgta ggggctcgcc tcgtgcagtt cctcgcgctg gccggggcga acgacgtcgt
     1801 gcggcggggc gacgacggac tcgagcgggc cggcgcgctc ggggtcgtag cgaagggcgc
     1861 ggaacggctt gacgaggctc acgggctgag gctatcgcac cgttcccgcg cccccgggag
     1921 cgtccgtcgg ggggcttaca ctcggagtgg tgagcgtcgc gctccagcac gccgtttcgc
     1981 ccggggcccg ggcggcgcgc gcgacgatcg ccgagctcgt cgaggaccgg atcgtcgagg
     2041 gcgtcttcgc cgtcgcgcgc aagcggcggc tctcgacccg cgcggggcgt ccgtacatcg
     2101 cgatcgagct cgtcgacgcg accgggcaga tcgagggacg cgtctggagc gacgtcgagc
     2161 tgctcgaccg ccggttccgc gagggtgacg cggtccgcgt gctcgcgcgg gtcgagcggt
     2221 tcaaggagcg gctccagctc gacgtcaggt cgctcgagcc ggtcgaggcg gacccggcct
     2281 cgctcgtccc gcaggcgcgg cgcgacctcg aggagctgga cggcttcctc gagttcattg
     2341 ccgaggagct cgcgcacccc ggcctccggc gcgtcgtgga cgggctcgtc ggcgactccg
     2401 cgttccgcga gcggatgcgg acgcttccgg cgacgccggg cgggcaccac gggtactcgg
     2461 gcgggctgct cgagcacacg gtcgcggtga cgacgctctg ccgcgacgcg gccgacctgc
     2521 acaagcggct gcggcgcgac ctcctgctcg ccgccgcgct gctccacgac gcaggccgga
     2581 cgctcgagct cgaccgtggg ccgctcgccc ggccgacgcc cgaggggcgg ctgctcggcc
     2641 acgtccatct cggcctgcgg ctgatcgagg agcgctcggc cgagctcgag gcggaggtgc
     2701 gcgccgagct gctccacgcg gtcgccgtcc accacgatcc gcgcgcggcc aggaccgccg
     2761 aggcgctcgt cctctaccac gcgaaccagc tcgacgccgt cgcggcgacg cggccggtcg
     2821 acggcgagta gcacccgtcc acgcgggtag cgtccgcgtg tggccgacga acggctcctg
     2881 cggatctacc tgaacgacca tctcgcgctt gcgacggcgg ccgtgcggct cgcgcgccgg
     2941 tcggcgaagt cgaacgcggg cagcgacctc gggcgcacgc tcgagcggat cgcggcggag
     3001 ctggacgacg agcggcacgt cttcgcgcgc gcggtcgagg cgctcggcta ccggcgcaac
     3061 cgggcgaagg aggccgcggc ggtcgtcggc gagcgcctcg ggctcctgaa gctgaacggc
     3121 cacgtcgtca gcccctcgcc gctcagccgg gtcgtcgagc tcgaggcgct cgccgctctc
     3181 gtcggctgga acgcccgtgc ctggcggacg ctcgccgggc ttccgcagct cgcggagcga
     3241 ctcgcaccgc tcgacctcga gcggctcgcg accgaggcgg acgagcggca ggccgagctc
     3301 gaccgcttcc ggccgggcgc ggcatccacg gcgttcgtcg gctgacgccc cgctagagtc
     3361 ccgcggtgcc cgttccccag gagcagctcg acgcgtacgc cgagctcgtc gtcggcgtcg
     3421 gcgtgaacct cgagcccgga caggcgttcg gcgtgacggc gtacgtcgag cacgcgccgt
     3481 tcgtccgcgc ggtcgcccgc gcggcgtggg cggcgggagc gcgggacgtg gacgtccgct
     3541 acgtcgacga gcacctccgc aaggcggcgg tccagctcgc gccggaggag tcgctcggcg
     3601 tctcgccgcc gtggctcctc gaccggcgcg agtcgtacgg cgaccgcagc gcctacctgc
     3661 tcgtgcgcgg ccatcccgac ccgcggatgt tcgaggacgt cgacggcgcg cgcctcgccg
     3721 cgtcgcggct gcacgaagag gacgcgatct ggctccggca ggtgagcgag cggcgcgtga
     3781 gctggtcgct cgtctcgttc ccgaccgagg gctgggcggc gacggtcttc ggcgagccgg
     3841 acgtcgggcg gctctgggac gcgattgccc gcacggtgcg gctggacgag cccgacccgg
     3901 tcgcggcctg ggccgagcac atccggcgcc tcgacgcacg cgcggcggcg ctgaacgagc
     3961 ggcgcttcga cgcgatccgc ttccgggggc cggggacgga cctcctcgtc ggcctgcacg
     4021 ccggctcgcg ctggcagagc gccggtgagg agaccgcgtg gggccgcccg cacgtcccga
     4081 acctcccgac ggaggagatc ttcacgacgc cggaccggcg gcggacggaa gggcgcgtgc
     4141 gctccacgcg gccgctggtc tgccagggga gaatcgtgcg ggggctcgag atcgagctcc
     4201 gcgccgggcg cgtcgtcgat gtgcgggccg aggagggcgc cgaggtcgtg caggcgcagg
     4261 tcgccacgga cgagggcgcg gccgagctcg gggagatcgc gctcgtcgac ggggactccc
     4321 gggtcgggcg ggaggggctc acgttcttcg acacgctctt cgacgagaac gcgagctcgc
     4381 acgtcgccta cggccagggc ttcggcgggt gcgtcgacgg cgcggcggag ctttcgccgg
     4441 aagagcagtg ggagctcggg atctcgcagt cgtcgctcca cacggacttc atggtcggcg
     4501 ggccggacgt cgagatcgac gggctgacgg cgtcgggcga ggcggtcgcg atcgtccgcg
     4561 gcgacgcctg gcagctctag ggcgtcgggc gggcgctcca ggtcttcggg tcttcgggtc
     4621 ttcggggctt cgcgacttcg ggacttcggg acttcgggac ttcgggattg ccaccggccc
     4681 cgggcctcga gccgcgcgct cgcccggccg gccttcctct cccaacgccc tgagacctac
     4741 cccacccccg gggggtcggg gtgcgacgct acaggtcgag cgcgcccgag acggtgacgt
     4801 tcgtgccggc ttcgtggcgg agccgtgccg attcccgcac acgggcgggt tcgcgaacgc
     4861 cctcagacgt tgaaccggat gttcaggacg tccccgtcct cgacctcgta cgtcttgccc
     4921 tcgagccgct ggaggccgcg cttcgccgcc tccgccgccg agccgcactc gacgagatcc
     4981 cgccagcgga tcacctcgca gcggatgaac ccgcgcgcga tgtccgtgtg aatctcggcc
     5041 gccgcgtcga gcgccgtctc tccccgccgg agcgtccacg cgcgggtctc cttgtcgccc
     5101 gcggtgaaga agctgatcag gtcgagcgcc gcgaagagcc gcgcgacgat ctcgccgagc
     5161 gccgacgggc cctcgcggaa cgccgccgcc tcgtcctccg gcagctcggc gagctcggcc
     5221 tcgagcttca ggtcgatccc gcccgggccg ttcacgacgg ggacgatcgg cttcgtcgtc
     5281 agcggctcga gcgcctccgg gaggccgccc gggtagtcgc ggagggcgcc gcccccgtcc
     5341 agatgcgcga ggagccgccg gagctcgtcg gcctcggcac gaagggccgc ctcgcccgac
     5401 ttcgcctgct tctcgacccg ctcgagccgc cgctcgacgt gctcccggtc ggcgaggagc
     5461 agctcgagct cgatcgtctc gaggtcggcg tccgggtcgg catccggccg gaagccgttc
     5521 agcaccgcga cgagggcatc gacctcgcgc aacccgccga gcagctgccg cccgctcccc
     5581 ggcacgtcga ccacgcgcac agcggcgggc gtcaccttcc gcgcggagac gaccgcggcg
     5641 acctcgccca gccgctcgtc cgcgatcggg gccatgccga cgttggcctt gtccgagacg
     5701 gtcgcgtacg cggtgatctc ggcgccggcg cgcgtcagcg cgttgaagag cgtcgtcttg
     5761 cccgcgttcg gcaagcccac gatgccggcc tggagcgcca cggccggagg ctagcgatct
     5821 aggcgtgcac gagccgctgc tgcagcgagc cgatcgcgcg gtgctggagc tgcttcacgg
     5881 cgttctcggt cttgccgagg atcgccgccg cctcgccgtt cgtgaaatcg aagacgtagc
     5941 ggagcgtcag gacctgctgc tggtcgcgcg agagcccgcg gatgagcccg agcatccgct
     6001 cgcggtcgat cgtgtggaac gccgcctcct cggccgaggg cgagacctcg gagatccgct
     6061 cgggcacgtc gccctccggc tgccagcggc ggccggcgcg gaagtggtcg atcgcgaggt
     6121 tgtgcgcgat ccggaagagc caggccgaga acggcacctt ctgccagacg aaccggtcga
     6181 gcgactcgag catgcggatg aacgtctggt tcgtcaggtc ttccgcgtcg tgctggctgc
     6241 cgacggagag acggaggtag ccgtagatcc ggtcgaagtg cgtccggtag agctcctcga
     6301 tcgccgcgcg gtcgcccgcc tgggcgcgct cgacgagccg ccgcacgtcc gggcccgcct
     6361 cctctgcgtc gccgcgcaca cggcgctcca cggtcgcttc cagtgtcctc gccaaccccg
     6421 ttgtcctctc tggacgcctc ccccggacgg ggtagtgcct ccactcgcac ctaagcagag
     6481 agctgccgaa aagcaacccg gcgcgcgacg cgccgcgggg gctacccggc ggcgccgaaa
     6541 cc
//
LOCUS       DASTFC010000041         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1615778,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000041 DASTFC010000000
VERSION     DASTFC010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615778"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VFO88_01200"
     CDS             complement(<1..1458)
                     /locus_tag="VFO88_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET9544171.1"
                     /translation="MTLERLQAPHMLVAALCLGLALPLAAPFGGRWLAILAGLLAVAA
                     PFASGRRRLLAATGALVLAGAWWGDARLASIDASTLESRVDESALARVEVTGPARHGL
                     FALRVPVRVLRFDRLTLNEPARLELPLERAPPQGAVLQVVATVERPRTAEEAGDFDEA
                     AYLARQGVHVILRAGWYRELGRRGGLPGAVDRLRHRLARSIAPGLEGERGALVAGVVL
                     GEDEKLSEPLRDDFRASGLYHLLAVSGQNVAYVVFGVLLFAWLAGLPRRAGHLAALAG
                     VLGYTCAVGWQPSVVRAGVAGTLASLAWLAARPSDRWYFWLVGAAVLLAVNPYSLLEP
                     GFQLSFAAVAGIFVLVPRIGRRLEGTPLPRVLREVVSVSLACSLVTAPILWIQFGSIP
                     VYSVLANALAFPVVAPLLGLALVCAALDPVLPSAALALAWVNGWLAAYLAWCARAVGS
                     LPHAEATSLRALLVIVGCVALAWTATRIRPPRAPRL"
     gene            complement(1455..2051)
                     /locus_tag="VFO88_01205"
     CDS             complement(1455..2051)
                     /locus_tag="VFO88_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9544172.1"
                     /translation="MEMPSISRPRALAYALVALALLVLGGRALTTGADGAPGAPPGGA
                     GLEVVEPEGEEAAEEVVVHVAGAVRQPGLYALEEGSRIDDAIARAGGPTGKAVLDAVN
                     LAAPVADGQQVVVPERGEVGLAGGGEAGGAATGGRVHLNTATLEQLDALPGVGPVTAQ
                     KILDYRDERGAFQSVDELDAVPGIGPARLEQLRELVDL"
     gene            complement(2141..3277)
                     /locus_tag="VFO88_01210"
     CDS             complement(2141..3277)
                     /locus_tag="VFO88_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET9544173.1"
                     /translation="MSPFDLLLVAWVLVFGLSGFFRGFAAQVVSLVGVGVGIVVGAWL
                     APHLLADGANGAWVPLASLVGAAIGAFVAGLVTGRVAARAHATLAANPVSNAVDRAGG
                     AAAGAVVGLALAWALAVLFLHQPELGLRQAVQRSAVLPALVRAVPPEPLLRTLERLDP
                     LPILGLGPGSLPDPDPSVLQSPAAAAAQWSVVRIEGSSCGLGIRGSGWVVRPEIVATN
                     AHVVSGQTDTRVLIPNEGTRDATIVYMDTRNDVALLRVPGLTAPPLPPATGDLYPRSV
                     VLLGYPRGGPLVARAGTAGEPRTVIAPDAYERSPAPRTVVPLRGQVQRGQSGGPVVAR
                     NGRVVAMVFGGSRGDDGGFGVPVELVLRGLDGPLEPVDPGRCVG"
BASE COUNT          495 a         1266 c         1311 g          311 t
ORIGIN      
        1 gagccggggc gcgcgcggcg ggcgaatccg cgtcgccgtc catgcgagcg ccacacaccc
       61 gacgatcacg aggagcgcgc ggagggacgt cgcctcggcg tgcggaaggc ttccgacggc
      121 gcgcgcgcac cacgcgaggt aggcggcaag ccatccgttc acccacgcga gcgcgagcgc
      181 ggccgagggc aggacggggt cgagcgccgc gcagacgagc gcgagcccga ggagcggcgc
      241 gacgaccggg aacgcgagcg cattcgcgag gaccgagtac acggggatcg acccgaactg
      301 gatccacagg atcggcgcgg tgacgaggct gcaggcgagc gagaccgaga cgacctcgcg
      361 caggacccgc gggagcggcg tcccttcgag cctccgcccg atccgcggaa cgagcacgaa
      421 gatcccggcg accgccgcga aggagagctg gaagcccggc tcgagcaggc tgtacgggtt
      481 gaccgcgagg agcaccgccg caccgacgag ccagaagtac cagcggtcgg acggccgcgc
      541 cgcgagccac gcgagcgacg cgagcgttcc ggcgactccc gcccgcacga ccgagggctg
      601 ccagcccacc gcgcacgtgt atccgagcac gcccgcgagc gccgccaggt ggcccgcccg
      661 gcgcggaagc ccggcgagcc aggcgaagag cagcacgccg aacacgacgt acgcgacgtt
      721 ctgtccggag acggccagca ggtggtagag cccggacgcg cggaagtcgt cgcgcagcgg
      781 ttcgctgagc ttctcgtcct cgccgaggac gactccggcg acgagcgcgc cgcgctcgcc
      841 ctcgagcccg ggcgcgatcg aacgcgccag gcggtggcgg aggcggtcga ccgcccccgg
      901 caacccgcca cggcggccga gctcgcggta ccagccggca cgcaggatga cgtgcacgcc
      961 ctgacgcgcg aggtacgcag cctcgtcgaa gtcgcccgcc tcctcggccg tccgcggccg
     1021 ctcgacggtc gcgacgacct ggaggacggc tccctgcggc ggcgcacgtt cgagcggaag
     1081 ctcgaggcgc gcgggctcgt tcagcgtgag ccggtcgaag cgcagcacgc ggacgggcac
     1141 ccggagggcg aagagcccgt ggcgcgcggg ccccgtgacc tcgacgcggg cgagggcgct
     1201 ctcgtcgacg cggctctcga gcgtgctcgc gtcgatggag gcgaggcgcg cgtctcccca
     1261 ccaggcaccg gcgaggacga gcgccccggt cgcggcgaga aggcgtcggc gaccggacgc
     1321 gaacggtgcg gcaacggcga ggaggccagc gaggatcgcg agccaccgtc cgccgaacgg
     1381 agcggcgagc gggagcgcga gcccgaggca gagcgccgcg acgagcatgt gcggggcctg
     1441 cagccgttcg agcgtcacag gtcgaccagc tcccgcagct gctcgagccg cgcgggcccg
     1501 atgccgggga cggcgtcgag ctcgtcgacc gactggaacg cgccgcgctc gtcgcggtag
     1561 tcgaggatct tctgcgcggt caccggcccg acgcccggga gcgcgtcgag ctgctcgagc
     1621 gtcgccgtgt tcaggtggac gcggccgccc gtcgccgcgc cgccggcttc tccgccgccg
     1681 gcgagaccaa cctcgccgcg ctcggggacg acgacctgct gtccgtccgc cacgggcgcg
     1741 gccaggttga ccgcgtcgag caccgccttc cccgtcgggc cgccggcgcg ggcgatcgcg
     1801 tcgtcgatcc ggcttccctc ctcgagcgcg tagagccccg gctgccgcac ggcgccggcg
     1861 acgtggacga cgacctcctc cgccgcttcc tcgccttcgg gctcgacgac ctcgaggccg
     1921 gcgccgccgg gcggagcgcc gggcgcgccg tccgcacccg tcgtgagggc ccgtccgccg
     1981 agaacgagga gggcgagcgc gacgagcgcg taggcgagcg cccggggacg cgagatcgag
     2041 ggcatctcca tgcgccggac gatgccgggc gaaagcgcac ggaaccgtca cgttcgtgcc
     2101 gagttcgtgt cggacccgtg ccggaccgcg cgcgggccgg tcagccgacg cagcggccgg
     2161 gatcgaccgg ctcgaggggc ccgtccaggc cgcgcaggac gagctcgacc gggacgccga
     2221 acccgccgtc gtctccgcgc gatccgccga agaccatcgc gaccacgcga ccgtttcgcg
     2281 cgacgaccgg ccctccgctc tggccgcgtt gcacctgccc gcgcaggggc accaccgtcc
     2341 gcggcgccgg ggagcgctcg tatgcgtcgg gcgcgatcac cgtgcgcggc tcccccgccg
     2401 tcccagcccg cgcgacgagc ggcccgcccc gcgggtagcc gagcaggacg acgctgcgcg
     2461 ggtagaggtc gccggtcgcg ggcggaagcg gcggcgccgt gagccccggc acgcggagga
     2521 gcgcgacgtc gttccgggtg tccatgtaca cgatcgtcgc gtcccgggtg ccctcgttcg
     2581 ggatcaggac gcgcgtgtcc gtctggccgg agacgacatg cgcgttcgtc gccacgatct
     2641 cggggcggac gacccagccg gagccgcgga tcccgagccc gcacgaggat ccctcgatcc
     2701 gcacgacgct ccattgcgct gccgccgcgg ccgggctctg gagcacgctc gggtcggggt
     2761 ccggcagcga cccgggcccg aggccgagga tcgggagcgg gtcgaggcgc tcgagcgtcc
     2821 gcaggagcgg ctccggcggc accgcacgga cgagcgccgg cagcacggca gaccgctgca
     2881 ccgcctggcg cagcccgagc tcgggctgat ggaggaagag gacggcgagc gcccaggcga
     2941 gcgcgagccc gacgaccgcg ccggcggccg caccgccggc ccggtcgacg gcgttcgaga
     3001 ccgggttcgc ggcgagcgtc gcgtgggcgc gggcggcgac ccggccggtg acgaggccgg
     3061 cgacgaaggc gccgatcgcc gcgcccacga ggctcgcgag cggcacccac gctccgttcg
     3121 cgccgtccgc aagaaggtgc ggggcgagcc aggcgccgac gacgatcccg acgccgaccc
     3181 cgacgagcga gacaacctgc gccgcgaagc cgcggaagaa cccggacagc ccgaagacga
     3241 ggacccacgc gacgagcagg agatcgaacg ggctcacgcg cgacgccccc gcggaggcgg
     3301 gccgaggcgg tccggcccgg cgtggagcgg caccggctca tggtacgtgc ggcgcacggg
     3361 ccggccacgg gcttgccatc gcg
//
LOCUS       DASTFC010000042         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_864,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000042 DASTFC010000000
VERSION     DASTFC010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFO88_01215"
     CDS             <1..504
                     /locus_tag="VFO88_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9544174.1"
                     /translation="LHADQFTEAGGIELAIELGARSVDHLEATGPVGVRALAANDVTA
                     VLLPIAALVLGRPQPPGRALVDAGAAVALATDLNPGSAFGESLPLACALACTQTGLAP
                     AEALAACTVNAAHVLGRADRVGRLAPGYDADVVLLDAPDWRYLAYHVSGCDVAVVVER
                     GEVAWRR"
     gene            545..967
                     /locus_tag="VFO88_01220"
     CDS             545..967
                     /locus_tag="VFO88_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544175.1"
                     /translation="MPSRKQRRRREKLKRHEYEYVVETEDGEEVHVERFRDAEPDRNA
                     PKAAGRPRNEVLVDRRGRPIQRPSLGRVLRRAAIFAPILFVFVYLTAQDRLTPAGMAF
                     NTLVLVAFFIPFSYLVDTMVYRMMIRRYERDQAAKRGR"
     gene            complement(973..1323)
                     /locus_tag="VFO88_01225"
     CDS             complement(973..1323)
                     /locus_tag="VFO88_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9544176.1"
                     /translation="MQTWRLPEIATPEGSRSPVVLRSENEARAVLVGLEPGQELGDHQ
                     VKENAWIVVVDGAAQFSAGEETVEAPVGTLVRFDPDERHSVRAPGGAKLLLLLAPWPG
                     PGHYRGEQRTAPES"
     gene            1368..2009
                     /locus_tag="VFO88_01230"
     CDS             1368..2009
                     /locus_tag="VFO88_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9544177.1"
                     /translation="MALDVTRLELGPIGTNCYLVRAPGSAEAVVVDPGADAAEIRLEL
                     ARSGAACAAILVTHGHWDHLGGVADLAEGTGAPVHMAEDERALLEHVNDYVPPGMRLR
                     PYTPDVLLAGAETLELAGLTFETLRVPGHSPAHLAFATEGALFSGDVLFAGSVGRADL
                     PGADWQLLLSSIATLAERFPPETVVYPGHGPATTIGDELETNPFLAELRASRR"
BASE COUNT          268 a          824 c          726 g          267 t
ORIGIN      
        1 ctccacgcgg accagttcac ggaggcgggc ggaatcgagc tcgcgatcga gctcggcgcg
       61 aggagcgtcg accatctcga ggcgacgggg cccgtcggcg tgcgcgcgct cgcggcgaac
      121 gacgtgaccg cggtgctgct cccgatcgcc gccctcgtcc tcggtcggcc ccagccgccc
      181 ggccgcgcgc tcgtcgacgc cggcgcggcg gtcgcgctcg cgaccgacct caacccgggg
      241 agcgcgttcg gggagagcct cccgctcgcg tgcgcgctcg cgtgcaccca gacgggcctc
      301 gcgccggccg aggcgctcgc ggcgtgcacg gtgaacgccg cgcacgtgct cgggcgcgcc
      361 gaccgcgtgg ggcgcctcgc gcccggctac gacgcggacg tcgtcctcct cgacgcgccc
      421 gactggcgct acctcgccta ccacgtgtcg ggctgcgacg tcgccgtcgt cgtcgagcgg
      481 ggcgaggtcg cctggcggcg ctgaaccgag gcggcagccg accgggcgcc gtcataatcg
      541 tctcatgccc tcgcggaagc agcggcgccg gcgcgagaag ctcaagcgcc acgagtacga
      601 gtacgtcgtc gagacggagg acggggagga ggtccacgtc gagcggttcc gcgacgccga
      661 gcccgaccgg aacgccccca aggccgccgg ccggcccagg aacgaggtgc tcgtcgaccg
      721 caggggccgg ccgatccagc ggccgagcct cggacgcgtc cttcgccggg cggcgatctt
      781 cgcgccgatc ctcttcgtat tcgtctacct gaccgcgcaa gaccggctca cgccggccgg
      841 gatggcgttc aacaccctcg tcctcgtcgc gttcttcatc ccgttcagct acctcgtgga
      901 cacgatggtc taccggatga tgatccgccg ctacgagcgc gaccaggccg ccaagcgcgg
      961 ccgctgagcg ccctacgact ccggcgccgt gcgctgctcg ccgcggtagt gcccgggccc
     1021 gggccacggc gcgagcagca ggagaagctt cgcaccgccc ggcgcgcgga ccgagtgccg
     1081 ctcgtccggg tcgaagcgca cgagcgtccc gaccggcgcc tcgaccgtct cctctcccgc
     1141 cgagaactgg gccgccccgt ccacgaccac gatccaggcg ttctccttca cctggtggtc
     1201 gccgagctcc tggcccggct ccagcccgac gagcacggcc cgcgcctcgt tctcgcttcg
     1261 caggacgacg ggcgagcggc tgccttccgg ggtcgcgatc tcggggagac gccacgtctg
     1321 cacgagcgcg ggaaacccta caggacccgg atagactcaa ccgcctgatg gccctggacg
     1381 tgacacgcct cgagctcggc ccgatcggga cgaactgcta cctcgtccgg gcgcccggct
     1441 cggcagaggc ggtcgtcgtc gacccgggcg cggacgcggc cgagatccgc ctggagctcg
     1501 cccggagcgg cgcggcctgc gccgcgatcc tcgtgacgca cggccactgg gaccacctcg
     1561 gcggagtcgc cgacctcgcc gagggaacgg gcgcgccggt gcacatggcc gaggacgagc
     1621 gcgcgctcct cgagcatgtc aacgactacg ttccgccggg gatgcggctg cgtccgtaca
     1681 cgcccgacgt gctcctcgcc ggcgccgaga cgctcgagct cgcgggcctc acgttcgaga
     1741 ccctccgcgt ccccggtcac tcgcccgcgc acctcgcgtt cgcgaccgag ggcgcgctct
     1801 tctccggcga cgtcctcttc gccggctccg tcgggcgcgc cgatctcccg ggcgccgact
     1861 ggcagctcct gctgtcgtcg atcgcgacgc tcgccgagcg gttcccgccc gagacggtcg
     1921 tctacccggg ccacggcccc gcgacgacca tcggcgacga gctcgagacg aaccccttcc
     1981 tcgccgagct ccgggccagc cggcggtgaa gctcgaggcg ccgaagggca cgctcgacgt
     2041 cctccccgcc gagcagccgc tgcgcgacgc cgtcgtcgac gccgc
//
LOCUS       DASTFC010000043         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_561097,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000043 DASTFC010000000
VERSION     DASTFC010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561097"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFO88_01235"
     CDS             complement(<1..638)
                     /locus_tag="VFO88_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HET9544178.1"
                     /translation="MRIAVVGGGSWGSVFAALLRERGHDVTLACRDAAQVAAIEETGH
                     NPRYVRGLSLAGIVPCTLEDPRLAEAELHVVAVPSGAFREVVAGLPDGGTPVLSLVKG
                     LDPGSGERLSRVVEGRPVAVLSGPNHAEEIADGLLAAAVIASEDEELANELQHAVHSE
                     RFRVYVNADLVGVELCAAAKNVIALGAGAVDGLGMGDNGKAALITRGLAEMSR"
     gene            complement(635..1966)
                     /gene="der"
                     /locus_tag="VFO88_01240"
     CDS             complement(635..1966)
                     /gene="der"
                     /locus_tag="VFO88_01240"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9544179.1"
                     /translation="MSAGVAEAPLLGSVAIVGFPNVGKSTLVNRLTATRQAVVFETPG
                     VTRDRKEIVCEWAGKRLLFVDTGGVDLADPSEITRQIAEQARRAVAEADLVLFAVDAR
                     AGVTPGDEEIADILRRAGKPVVVVANKVDDPARDLDALEFHRLGLGDPVAISALHGHG
                     TGDLLDRLVADLERLGPAPRPEVGEEAIRVAVLGRPNVGKSSLLNAIAGEERVIVSDV
                     PGTTRDSIDTVVRRGEQTFVFVDTAGLRRKRRQRQGIEYYSELRALQAAERADVALVL
                     VDASEGLVDGDLAVADVARTAGCSTLVVLSKWDETVVEVEDVRPRLDERLRQRPPLVA
                     VSAHTRRGLHRLLERIEELFARHTSRVQTAGLNTFLQELREQRPGPSKGGKRLNLLYG
                     TQVSVRPPRFRIFVNDPGLMTRDYGYWVENRLRERFGLAGVPVVIDFVRRS"
     gene            complement(1963..2649)
                     /locus_tag="VFO88_01245"
     CDS             complement(1963..2649)
                     /locus_tag="VFO88_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9544180.1"
                     /translation="MSATRAAHAFMWWTVGKGILASATRLVVPLKDYGRERIPREGGV
                     VLAVNHLAWIDVPAIGTLCPRRIVFLAKAEAHEVPGFGQLMRSMGTLSVRRGESDREA
                     LRLAREAVRANGLLGVFVEGTRQAGRPGEAKPGAAMIAMQEGVPVLPAAVYGSYEWTP
                     RNRNPVSVAWGEPMRFEHLPRNSRGYKEATTEIQAEILRLWSFLVDMHRLGRPDGTPP
                     RRAELPSRAG"
     gene            complement(2646..3284)
                     /gene="cmk"
                     /locus_tag="VFO88_01250"
     CDS             complement(2646..3284)
                     /gene="cmk"
                     /locus_tag="VFO88_01250"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9544181.1"
                     /translation="MIIAIDGPAGAGKSTVARLLAKRLGFRYLDTGAMYRALTWLALE
                     RGVSLDDADALAGLARAHDVTVDGDSVEIAGHEIGAAIRTVEIDRVVSSVARHDPVRE
                     ILRERQRTLAAEGDAVMEGRDIGMVVCPDADVKIFLTADPEERARRRTDERPGLGADA
                     LATDLRLRDRRDAKQLEAAPDAERIDTTHLGIEEVVARIEALAAARAAEAKP"
     gene            complement(3307..>4011)
                     /locus_tag="VFO88_01255"
     CDS             complement(3307..>4011)
                     /locus_tag="VFO88_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544182.1"
                     /translation="ERLLEAAGARIRRTPRRAAVWPAERLRPLDLDVPGDFSSAAPFV
                     VAATLLSGSDLRIHGVGINPTRTGFLSVLDRMGARVSLFNRRAAGGEPIADVEVAPAE
                     LVATEIRPEEVPLLVDELPLFALVAGLARGESTVTGAQELRVKESDRLEAVKEVLRPL
                     GVRIQTTEDGFRVRGVPARPRGGVSVDAHGDHRIAMLAAVAGAVSREGVEISGAESVA
                     VSYPEFFAVLDSLALR"
BASE COUNT          512 a         1487 c         1514 g          498 t
ORIGIN      
        1 cggctcatct ccgcgagccc gcgcgtgatg agcgccgcct tgccgttgtc gcccatcccg
       61 agcccgtcga cggccccggc gccgagcgcg atcacgttct tcgcggccgc gcagagctcg
      121 acgccgacga ggtcggcgtt cacgtacacg cggaagcgct ccgagtggac ggcgtgctgg
      181 agctcgttcg cgagctcctc gtcctcgctc gcgatcaccg ccgccgcgag cagcccgtcc
      241 gcgatctcct ccgcgtggtt cgggcccgag agcaccgcga ccggccgccc ttcgacgacg
      301 cgcgacagcc gctcgcccga gcccgggtcg aggcccttca cgaggctgag gacgggcgtc
      361 ccgccgtcgg ggagcccggc gacgacctcc cgaaaggcgc cgctcgggac ggcgaccaca
      421 tgcagctcgg cctccgccag gcgcgggtcc tcgagcgtgc agggcacgat cccggcgagc
      481 gagaggccgc gcacgtagcg cgggttgtgc cccgtctcct cgatcgcggc gacctgcgcg
      541 gcgtcgcggc acgcgagcgt cacgtcgtgc ccgcgctcgc gcaggagcgc cgcgaacacg
      601 gagccccacg agccgccgcc gacgaccgcg atcctcacga gcgccgcacg aagtcgatca
      661 cgacggggac gcccgcgagg ccgaagcgct cgcgcagccg gttctcgacc cagtagccgt
      721 agtcgcgcgt catcaggccg ggatcgttca cgaagatgcg gaagcgcggc gggcgaacgc
      781 tcacctgcgt cccgtagagg aggttcaggc gcttcccgcc cttcgacggc ccggggcgct
      841 gctcgcggag ctcctggagg aacgtgttca gcccggccgt ctgcacacgc gaggtgtggc
      901 gggcgaagag ctcctcgatg cgctccagca gccggtgcag gccgcgccgg gtgtgcgcgg
      961 agacggccac gagcggcggg cgctggcgca ggcgctcgtc gaggcgcggg cggacgtcct
     1021 cgacctccac cacggtttcg tcccacttgg agaggacgac gagcgtcgag cacccggccg
     1081 tccgggcgac gtccgcgacg gccaggtcgc cgtccacgag cccctcggag gcgtcgacga
     1141 ggacgagcgc gacgtcggcg cgctcggccg cctggagcgc acgtagctcc gagtagtact
     1201 cgatcccctg gcgctggcgg cgcttccggc gcaggccggc cgtgtcgacg aagacgaacg
     1261 tctgctcgcc gcggcggacg accgtgtcga tcgagtcgcg cgtcgtgccc gggacgtcgg
     1321 agacgatcac gcgctcctcg cccgcgatcg cgttcaggag gctcgacttg ccgacgttcg
     1381 gccggccgag gacggccaca cggatcgcct cctcccccac ctccggccgc ggcgcggggc
     1441 cgagtcgctc gaggtctgcg acgagccggt cgagcaggtc gccggtgccg tgcccgtgca
     1501 gcgccgagat cgcgaccggg tcgccgagcc cgagccggtg gaactcgagc gcgtcgaggt
     1561 cgcgcgccgg gtcgtccacc ttgttcgcga cgacgacgac cggcttcccc gcgcggcgca
     1621 ggatgtccgc gatctcctcg tcgcccggcg tgacgcccgc gcgcgcgtcg accgcgaaga
     1681 ggacgaggtc ggcctccgcg accgcgcggc gtgcctgctc ggcgatctgg cgcgtgatct
     1741 cggacgggtc ggcgaggtcg accccgccgg tgtccacgaa gaggaggcgc ttcccggccc
     1801 actcgcagac gatctccttg cggtcgcggg tcacgccggg cgtctcgaag acgaccgcct
     1861 ggcgcgtcgc cgtgagccgg ttcacgagcg tcgacttgcc cacgttcggg aacccgacga
     1921 tcgccaccga cccgagcagc ggcgcctcgg cgacgcccgc gctcatccgg cgcgcgacgg
     1981 gagctcggcg cggcgcggcg gcgtcccgtc tggccggccg agccggtgca tgtccacgag
     2041 gaagctccag aggcggagga tctcggcctg gatctcggtc gtcgcctcct tgtagccgcg
     2101 cgagttgcgc gggaggtgct cgaagcgcat cggctcgccc cacgcgaccg agacggggtt
     2161 ccggttccgc ggcgtccact cgtacgagcc gtagaccgcg gccggcagga cgggaacgcc
     2221 ctcctgcatg gcgatcatcg ccgcgcccgg cttcgcctcg ccggggcggc cggcctggcg
     2281 cgtgccctcg acgaagacgc cgagcagccc gttcgcgcgg acggcctcgc gggcgagccg
     2341 gagggcctcg cggtcgctct ctccgcggcg cacggagagc gtccccatcg agcgcatgag
     2401 ctggccgaag cccgggacct cgtgcgcctc cgccttcgcg agaaagacta tccggcgcgg
     2461 gcagagggtc ccgatcgcgg ggacgtcgat ccaggcgagg tggttcacgg cgaggacgac
     2521 gccgccctcg cgcggaatgc gctcgcggcc gtagtccttg agggggacga cgagccgggt
     2581 ggcgctcgca aggatgccct tgccgaccgt ccaccacatg aacgcgtggg ccgctcgggt
     2641 cgcgctcacg gcttcgcctc cgcggcgcgg gcggcggcga gcgcctcgat gcgggcgacg
     2701 acctcctcga tcccgaggtg cgtcgtgtcg atccgctcgg cgtcgggcgc ggcctcgagc
     2761 tgcttcgcgt cgcgcctgtc gcgcaggcgc aggtcggtcg cgagcgcgtc ggcgccgagg
     2821 cccggccgct cgtcggtgcg ccggcgcgcg cgctcctcgg gatcggcggt gaggaagatc
     2881 ttcacgtcgg cgtcggggca gacgaccatg ccgatatccc ggccctccat gactgcgtcg
     2941 ccctcggcgg cgagcgtgcg ctggcgctcg cggaggatct cgcgcaccgg gtcgtggcgg
     3001 gcgacggagg agacgacgcg gtcgatctcg accgtgcgga tggcggcgcc gatctcgtgc
     3061 ccggcgatct cgacggagtc gccgtccacg gtcacgtcgt gcgcgcgggc gagacctgcg
     3121 agcgcgtcgg cgtcgtcgag cgagacgccc cgctcgagcg cgagccacgt gagcgcgcgg
     3181 tacatcgcgc ccgtgtcgag gtagcggaag ccgagccgct tcgcgaggag ccgcgcgacc
     3241 gtgctcttcc cggccccggc cgggccgtcg atggcgatga tcatccgcgg ctccgcacgg
     3301 gctgcatcat cgcagcgcga gcgagtcgag cacggcgaag aactcgggat acgagacggc
     3361 gaccgactcg gcgccgctga tctcgacgcc ctcgcgggac acggcgcccg cgaccgcggc
     3421 cagcatcgcg atccggtggt cgccgtgcgc gtcgaccgag acgccgccgc gcggccgcgc
     3481 cggaacgccg cgcacgcgga acccgtcctc ggtcgtctgg atgcgcacgc cgagcgggcg
     3541 gagcacctcc ttcacggcct cgaggcggtc ggactccttc acgcggagct cctgcgcgcc
     3601 ggtgaccgtg ctctccccgc gcgcgaggcc ggcaacgagc gcgaagagcg ggagctcgtc
     3661 gacgaggagc ggcacctcct cggggcggat ctcggtcgcg acgagctcgg cgggcgcgac
     3721 ctcgacgtcg gcgatcggct cgccgcccgc ggcgcggcgg ttgaagagcg agacgcgcgc
     3781 gcccatccgg tcgagcaccg agaggaaccc ggtgcgggtc gggttgatgc cgacgccgtg
     3841 gatgcgcagg tcgctcccgc tcaggagcgt cgcggcgacg acgaacggcg ccgcggagga
     3901 gaagtcgccc gggacgtcga ggtcgagcgg gcggaggcgc tccgccggcc agacggcggc
     3961 ccggcgcggg gtgcggcgga tccgcgcgcc ggcggcctcg aggagccgct c
//
LOCUS       DASTFC010000044         9478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1302792,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000044 DASTFC010000000
VERSION     DASTFC010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302792"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFO88_01260"
     CDS             <1..481
                     /locus_tag="VFO88_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9544183.1"
                     /translation="ARREAASMAEIAAEADITRGTLYRHFPGRETLVHALEAAANDEA
                     GRRLAEANLDQVPVSEGLARATRALVTVGADFVVLLRERRPPEPAFTAPLEALLERGR
                     EEGQLRTDVSVATLVESLLALVAVSVRTGRQEGAGLEDMSATALRLFLTGARAPDQN"
     gene            complement(704..1171)
                     /locus_tag="VFO88_01265"
     CDS             complement(704..1171)
                     /locus_tag="VFO88_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9544184.1"
                     /translation="MLAALVLMLDVLPSSAQVSPPPIATEFLTERAVFTDDVDTKFKI
                     KMDGRKTTVVKDKNPSRTVVARFTVQPGAQFPWHSHPGPVIVNVVQGELTYLPSSDCV
                     ERTYGAGTAFVDPGHGHTHTAWNATGGVTVFVATFFAAPATGPLLIPASQGCP"
     gene            1867..3693
                     /locus_tag="VFO88_01270"
     CDS             1867..3693
                     /locus_tag="VFO88_01270"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9544185.1"
                     /translation="MGLSIHVLGPPRLERGGAPAEPPRGNKAWGLLVFLLRTRLPPSR
                     ERLAGLLFPEADDPLGTLRWTLSELRRSLGDDADVGGDPVRLTLHPGTLVDVDVLNGG
                     SWMEAVALPGFGHELVDGLTVRSSPGFEMWLEDERRHVAGTTSAVLHQAALALLGRGQ
                     AADAVRHASELVRLNPYEENAHVLLVRCLRAAGDPEGAASRAEACTELFRRELGIEPS
                     GALRTAAEASEAPVVGRVSSGAAVLAQIEAGEAALAAGAVDAGVQRMRGAVAAARTID
                     ERELLARALVSLGGALVHSARGRDEEGAASLHEGVTLAEQAGRADVAATGWREISWVQ
                     FLRAQYERAEASLTRTAELAAGANEELAWVDLTRGACRHDTGDYAAAGELLRSGVERA
                     RRLPSGQPLAQALTLLGRYHLLRGEIEDAVHVLDQALAEVDARGMTAFVSWPESFRAE
                     VDLALGDVDAAEARFEHAFALGCQVGDACWESIGLRGLGLVAAARGDVPRALDLLVEA
                     PRLCRRLPDTYLWIEAYALDALCAVAVEHRAEAALRWIDELEAVTARRGIRELLVHAT
                     IYRARLGEPGAAEAARSLAAQVDNPALDGLLGLQELAADASY"
     gene            complement(3690..4208)
                     /locus_tag="VFO88_01275"
     CDS             complement(3690..4208)
                     /locus_tag="VFO88_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544186.1"
                     /translation="MSGAAHTEGRAGRLARGERLAGFIYGTIIVLSVIAAGAKAYPDS
                     AGHVAALVLATTTVLWLAHVYAHGLGQSVARDERLSAAELGHIARLEVSIVGAAVLPV
                     AALLLDPLGLASATVAGWLAFGLGVAALVAQGVGFGRMERLSPLGTLAVVALNLCIGL
                     VLVALKLFVSHY"
     gene            4358..4567
                     /locus_tag="VFO88_01280"
     CDS             4358..4567
                     /locus_tag="VFO88_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544187.1"
                     /translation="MRKDVREFIRRLEAAGLTVEPTPGHYRVLRDGKPLRKANGMPFM
                     LPFSPDTTRWRRSATVELRKLGIDL"
     gene            4654..5229
                     /locus_tag="VFO88_01285"
     CDS             4654..5229
                     /locus_tag="VFO88_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9544188.1"
                     /translation="MTNLAIADAHRSAEEALLDAAERLLVDVGYARITTRRLAETAGV
                     NHGLVHYYFGSNENLLVRALERFTERLVERQRALYAGDEPFPEKWRTAMRYLVDDDAH
                     YEKIWLELQALSWNDAGLRERLARVNAEWRAVLTEAFAEPHRELGIPMPLDALVTLVM
                     TFNLGLIVERLGGVTAGHDTLLDWIDGWLSG"
     gene            5214..7325
                     /locus_tag="VFO88_01290"
     CDS             5214..7325
                     /locus_tag="VFO88_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9544189.1"
                     /translation="MAVRLTAAPQPEQTRARYPDESGHVERDGVRIHYEVYGAGEATV
                     LLLPTWSIIHSRHWKMQIPYLARHCRVVTFDGRGNGHSDRPEAGYDEHEFAADALAVL
                     DATGTRSATLVSLSLGAQRALILAAEHPERVDAAVFICPAVPLGDPPPGRGIHAWDEE
                     LATDEGWAKYNRYHWLRDYRDFLEFFFSRMFTEPHSTKPIEDCVGWGLETTPETLVTA
                     ELGRGLDAETTRGLAARLTCPVLVIQGTEDAITGPARGIELAEATGGELVLLEGSGHG
                     PHVRDPVKVNLLLREVVAPPPRPTRWLRGRARRKRALYVSSPIGLGHAQRDVAIADEL
                     RLLHPDLEIDWLAQHPVTAVLEGRGERIHPASAHLANESGHIESESAEHDLHCFQAIR
                     RMDEILLANFMVFHDLVRDEHYDLWIGDEAWELDYYLHENPEQKGAAYVWLTDFVGWL
                     PMEDGGDREAFVTADYNAEMIEHIARFPRLRDRAVYVGNSDDIVPDRFGPGLPAIRDW
                     TEENFDFAGYVTGFDPRDVGDRGALGYGDDERVCIVTVGGSGVGAHLLRRVIAAFPEA
                     KERVPDLRMVVVAGPRIDPASLPEHDGLEVRAYVHELYRHLAVCDLAIVQGGLTTAME
                     LTANRRPFLYFPLRHHFEQNFHVRHRLERYGAGRCMDFETDGPPEIAAAIAEEIGREV
                     DYRPVETDGAARAARLIAELL"
     gene            complement(7372..7926)
                     /locus_tag="VFO88_01295"
     CDS             complement(7372..7926)
                     /locus_tag="VFO88_01295"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HET9544190.1"
                     /translation="MKPFIKVGALSVAVIAAAVLAAAAYAGPAAPDVPEAIAVPEGNK
                     VFLVGEAVGVQIYACNAVAGGYRWDFVAPRANLYDERGMLLTTHFGGPSWQARDGSLV
                     VGTVVDRAPVAGAIPWLLLAAASTSAGADGDRLAHTTFIQRIATTGGLAPAEASCHAG
                     TVGTQAEVPYTADYYFWKATGSGS"
     gene            complement(8045..8653)
                     /locus_tag="VFO88_01300"
     CDS             complement(8045..8653)
                     /locus_tag="VFO88_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544191.1"
                     /translation="MILFRMLVVVTAAAVLSACGGDSEPAATAPAATTEASDPREGYF
                     TSVESEALNPSLGSFSGAWSSYRARGEACNEEAQRVFDEGASPRRAVRCHLEENRALI
                     DATDEVRAAFEGLSGEYREECEAGVERFTSALDEVESLRQEVLDGYERYAETGEVARG
                     FRRESAEADERTQALLAAELVALTEACYTEEDREQAAEDADG"
     gene            complement(8783..>9478)
                     /locus_tag="VFO88_01305"
     CDS             complement(8783..>9478)
                     /locus_tag="VFO88_01305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9544192.1"
                     /translation="AIDAARGDWVAVLDADDAWLPERLERLTPLLAEADVVTDDIVIE
                     EAGKRTRRLLASHGVRAAEPFELRPADLARYGLGLLKPLVRRTFVRDHGIRYDGSLPV
                     GSDFCFFVDLALAGARWIQVPDAYYLYRRRAGSVTGDRREHLQGRLASDSLLLARPGV
                     QGHAELRGLLEDRVGWLRDLDRLLEAHERGGRALARAVVADPRLARTGARHGYHVLRR
                     RVRAARRPRDAAG"
BASE COUNT         1318 a         3534 c         3385 g         1241 t
ORIGIN      
        1 cgcccggcgg gaagcggcga gcatggccga gatcgcggcc gaggcggaca tcacgcgcgg
       61 gacgctctac cgccacttcc cgggccgcga gacgctcgtg cacgccctcg aggccgccgc
      121 gaacgacgag gccggacggc gcctcgcgga ggcgaacctc gatcaggtcc cggtgtccga
      181 ggggctcgcg cgcgcaactc gcgccctcgt cacggtcggc gccgacttcg tcgtcctcct
      241 ccgtgaacgg cgaccccccg agccggcctt cacggccccg ctcgaggcgc tcctcgagcg
      301 cggtcgcgag gagggccagc tgcggacgga cgtctccgtc gccaccctcg tcgagtcgct
      361 cctcgccctc gtcgcggtga gtgtcaggac cggcaggcag gaaggagcgg gcctcgagga
      421 catgagcgcg accgctctcc ggctcttcct caccggcgcg cgcgcccccg atcagaactg
      481 agcgtcgccg cgccagccgg acgggagggc gccgtggccc ggcgccctcc cgtacggcaa
      541 cctgactcgg tcagcggccg ccgcccttgg cccgcgtcgt gacgccagat ctcggccctt
      601 ttgaggtagt cgccgaagat acgaccatga ggacgggact atctcggccc gtcctcatgg
      661 ctttcggctc tcgctgtccg taccgctttc gcggttccga cggctacggg cagccctggg
      721 aggcggggat gagcagcggc cctgtggcgg gcgcggcgaa gaacgtcgcc acgaacaccg
      781 tcacgccgcc cgtcgcgttc caggcggtgt gggtgtgccc atgaccggga tcgacgaagg
      841 cggtgccggc gccgtacgtg cgttcgacgc aatcgctcga aggcaggtag gtcagctccc
      901 cctggacgac gttgacgatc acggggccgg ggtgcgagtg ccaggggaac tgcgctcccg
      961 gctgcaccgt gaatctcgcg acgaccgttc gcgagggatt cttgtccttc acgacggtgg
     1021 tcttccttcc gtccatcttg atcttgaact tggtgtccac gtcgtcggtg aacaccgcac
     1081 gctcggtcag gaactccgtc gcgatgggcg gcggcgaaac ctgcgccgac gacggcagca
     1141 cgtcgagcat gaggacgagg gcggcgagca acgccgctcc tcctgcaata gctcctcgct
     1201 taccggtcat gtctttctct ccttcgttgg atgttcgacc ttgggcgcgc ggcgacgcgc
     1261 ccggccttgc tcagcccgcg cggcccggac gttgccctgg catcgccttg cctagcaagc
     1321 gtcacgcggc tcatgcgcgt agggggcgac gacgcctgcg ccgaagttcg gctggttcgc
     1381 gaactgcgct ccggcgcgga tgtcggctcc gaaaagcgcg ccgtcctgct gggcgttcgc
     1441 cgacgcaaac tggccgacgc acgacggggt tcccgcggca acgggcgcac ccggtagggc
     1501 gaggaaagct gccgcagata cgaaagcgac aacacgtgtc cgcatgatgc tttctccttc
     1561 cgcagaggga cgcacgatcg attcccgaac ccgtgaacgg cggcctcgct tcctcatcac
     1621 cgccccgggt tcacagcgcc tgacgccgag ggcgcaagag cgctgacaac accgagcgtc
     1681 gcgaggagca gaaccgttac tcgacgtcgc agagcatcct cctttccatt ctccccacga
     1741 gcgcgaggcg agccgatctc ctgtggacgt tcatgccgca ctcccttcgt acgccgtatt
     1801 gcgcttgacg ggacggaggt tcacagtgag gcgtgtgacg ggacgtgtga ggacggaggg
     1861 cgctcggtgg ggctgagcat ccacgtcctc gggccgccga ggctcgagcg cggcggcgcc
     1921 cccgccgagc ccccgcgcgg caacaaggcg tgggggctgc tcgtcttcct cctgcgaaca
     1981 cggctgccgc cctcgaggga gcggctggcc ggcctcctct tcccggaggc ggacgacccg
     2041 ctcggaacgc tccgctggac gctctcggag ctccggcgca gcctgggcga cgacgcggac
     2101 gtcggcggcg acccggtgcg cctgacgctg cacccgggga cactcgtgga cgtcgacgtc
     2161 ctgaacggag gctcgtggat ggaggccgtc gcgctgcccg gcttcggcca cgagctggtg
     2221 gacggcctca ccgtccgctc cagccccggc ttcgagatgt ggctcgagga cgagcgccgc
     2281 cacgtcgccg ggacgacgag cgccgtgctc catcaggccg cgctcgcgct gctcggacgc
     2341 gggcaggccg ccgacgccgt ccgccacgct tcggagctcg tgcgactgaa cccgtacgag
     2401 gagaacgccc acgtgctcct cgtgcgctgc ctgcgcgccg caggcgaccc cgaaggtgca
     2461 gcaagcaggg ccgaggcctg cacggagctc ttccgccgcg agctgggcat cgagccgagc
     2521 ggcgccctgc ggaccgcggc cgaggcgtcc gaggctcccg tcgtgggccg cgtctcgagc
     2581 ggcgccgccg tcctcgcgca gatcgaggcg ggcgaggccg ccctcgccgc gggcgcggtc
     2641 gacgccggtg tccagcgcat gcgaggcgcg gtcgccgcgg cgcggacgat cgacgagcgc
     2701 gagctcctcg cgcgcgcgct cgtgtcgctg ggcggcgcgc tcgtccactc ggcccgcgga
     2761 agggacgagg agggtgcggc ctccttgcac gagggggtga cgctcgccga gcaggccggc
     2821 cgggccgacg tcgccgcgac cgggtggcgc gagatcagct gggtgcagtt cctgcgggcc
     2881 cagtacgagc gcgcggaggc gtcgctgacc cgcacggcgg agctcgcggc gggagcgaac
     2941 gaggagctcg cctgggtcga cctgacgcgg ggcgcctgcc ggcacgacac cggcgactac
     3001 gcggcggccg gcgagctcct ccgctcgggg gtcgagcggg cgaggcggct cccgtccggc
     3061 cagccgctcg cccaggcgct cacgctgctc gggcgctacc acttgctccg tggggagatc
     3121 gaggacgcgg tccacgtcct cgaccaggcg ctcgcggagg tcgacgcgcg cggcatgacc
     3181 gcgttcgtct cctggccgga gtccttccgc gccgaggtcg acctcgccct cggcgacgtg
     3241 gacgccgcgg aggcgcgctt cgagcacgct tttgcgctcg gctgccaggt tggcgacgct
     3301 tgctgggaga gcatcggcct gcggggtctc ggactcgtcg cggcggcgcg cggcgacgtc
     3361 ccccgcgcgc tcgacctgct cgtcgaggcg ccgcggctct gccgccggct tccggacacg
     3421 tacctctgga tcgaggccta cgcgctcgac gccctctgcg ccgtcgccgt cgagcatcgc
     3481 gccgaggccg ccctgcgctg gatcgacgag ctcgaggcgg tcacggcgcg gcgcgggatc
     3541 agggagctcc tcgtgcacgc gacgatctac cgggcccggc tgggcgagcc cggcgccgcc
     3601 gaggcggccc gctcgctcgc cgcgcaggtc gacaacccgg cgctcgacgg gctcctcggg
     3661 ctgcaggagc tcgccgccga cgcctcctac tagtagtggg agacgaacag cttgagcgcg
     3721 accagcacga ggccgatgca caggttcagg gcgaccacgg cgagcgtccc gagggggctg
     3781 agccgctcca tgcggccgaa cccgacgccc tgtgcgacga gcgcggccac gcccagcccg
     3841 aaggcgagcc accccgcgac cgtcgccgag gcaaggccga gcgggtcgag caggagcgca
     3901 gcgacgggca ggacggcggc cccgacgatc gacacctcga gccgggcgat gtggccgagc
     3961 tcggccgcgg acaggcgctc gtcgcgcgcg acgctctggc cgagtccgtg cgcgtacacg
     4021 tgcgcgagcc agagcacggt cgtcgtggcg agcacgagcg cggcgacgtg cccggccgag
     4081 tccggatacg ccttcgcgcc cgccgcgatc acggacagga cgatgatcgt gccgtagatg
     4141 aagcccgcga gccgctcgcc tctcgccagc cggcccgcgc ggccctcggt gtgggcggcg
     4201 ccgctcacgc gcgcatcatg ctggtcacgg agccgccacg cgagcccgct gccccgccaa
     4261 gaggaggaga cggacagccg gctgcgccgg ctcggcctgc gaagctgagg cgtccgtcga
     4321 acgaggagcg aatggaggga acgcgtttgc gccggccatg aggaaggacg tgcgcgagtt
     4381 catccgccgg ctcgaggcgg ccgggctcac cgtcgagccg acgcccggcc actaccgcgt
     4441 cctgcgcgac ggcaagccgc tccggaaggc gaacggcatg ccgttcatgc tccccttctc
     4501 gcccgacacg acccgctggc gacgctcggc gaccgtggag ctccgcaagc tcgggatcga
     4561 cctctgagac ccccgcagag cagcgcagac tgacggcgcg ggcacccttg acgtggccct
     4621 tcaggtgcgg aacaattcga gcggtttagt caaatgacta atcttgccat cgccgacgca
     4681 catcgatcgg ccgaggaagc gctcctcgac gccgccgagc gtctgctcgt cgacgtcggc
     4741 tacgcgcgca tcacgacccg ccgcctcgcc gagaccgcgg gcgtgaacca cgggctcgtc
     4801 cactactact tcggctcgaa cgagaacctg ctcgtgcggg cgctcgagcg cttcaccgag
     4861 cgcctcgtcg aacgccagcg cgcgctctac gccggcgacg agccgttccc ggagaagtgg
     4921 cgcaccgcga tgcgctatct cgtggacgac gacgcgcact acgagaagat ctggctcgag
     4981 ctccaggcgc tctcctggaa cgacgccggc ctgcgcgagc ggctcgcccg cgtgaacgcg
     5041 gagtggcgcg ccgtcctcac ggaggcgttc gccgagccgc atcgggagct cgggatcccg
     5101 atgccgctcg acgcgctcgt cacgctcgtc atgacgttca acctcgggct catcgtcgag
     5161 cgcctcggcg gcgtgaccgc cggccacgac acgctcctcg actggatcga cggatggctg
     5221 tccggctgac cgccgcgccg cagccggagc agacgcgcgc ccgctacccg gacgagtccg
     5281 gtcacgtcga gcgcgacggt gtccggatcc actacgaagt ctacggcgcc ggcgaggcga
     5341 cggtgctgct cctgccgaca tggtcgatca tccattcgcg ccactggaag atgcagatcc
     5401 cgtatctcgc gcgccactgc cgggtcgtca ccttcgacgg ccgcgggaac gggcacagcg
     5461 accggccgga ggcgggctac gacgagcacg agttcgccgc cgacgcgctc gcggtcctcg
     5521 acgcaaccgg cacgcgaagc gcgacgctcg tctcgctctc gctcggcgcg cagcgggcgc
     5581 tcatcctcgc ggcggagcat ccggagcgcg tcgacgcggc cgtcttcatc tgcccggccg
     5641 tcccgctcgg ggacccgccg cccggccgcg gcatccacgc ctgggacgag gagctcgcga
     5701 ccgacgaggg ctgggcgaag tacaaccgct accactggct gcgcgactac cgggacttcc
     5761 tcgagttctt cttctcgcgg atgttcacgg agccgcactc gacgaagccg atcgaggact
     5821 gcgtcggctg ggggctcgag acgacgcccg agacgctcgt caccgccgag ctcggccggg
     5881 ggctcgacgc ggagacgacc cgcgggctgg ccgcgcgcct cacctgcccg gtgctcgtga
     5941 tccagggaac ggaggacgcg atcaccggcc cggcgcgggg gatcgagctc gcggaggcga
     6001 ccggcggcga gctcgtgctg ctcgagggct ccgggcacgg gccgcacgtg cgcgacccgg
     6061 tcaaggtgaa cctcctgctg cgcgaggtcg tcgcgccgcc gccccgtccc acgcgctggc
     6121 tccgcggcag ggcccgccgc aagcgggcgc tctacgtctc ctcgccgatc gggctcggcc
     6181 acgcgcagcg cgacgtggcg atcgccgacg agctacggct gctccatccc gacctcgaga
     6241 tcgactggct cgcccagcac cccgtcacgg ccgtgctcga ggggcgcggc gagcggatcc
     6301 acccggcgag cgcgcacctg gcgaacgaat cggggcacat cgagagcgag tcggccgagc
     6361 acgacctcca ctgcttccag gcgatccggc ggatggacga gatcctgctc gcgaacttca
     6421 tggtcttcca cgacctcgtc cgcgacgagc actacgacct ctggatcggc gacgaggcct
     6481 gggagctcga ctactacctg cacgagaacc ccgagcagaa gggcgccgcg tacgtctggc
     6541 tcacggactt cgtcggctgg ctcccgatgg aggacggcgg cgaccgggag gccttcgtga
     6601 cggccgacta caacgcggag atgatcgagc acatcgcgcg cttcccgcgc ctccgcgacc
     6661 gcgccgtgta cgtcgggaac tcggacgaca tcgtccccga ccgcttcggg ccggggctcc
     6721 cggcgatccg cgactggacg gaggagaact tcgacttcgc cggctacgtg accgggttcg
     6781 acccgcggga cgtcggtgac cgcggcgcgc tcggctacgg cgacgacgag cgcgtctgca
     6841 tcgtgaccgt gggcggctcg ggcgtcggcg cgcacctgct ccggcgcgtg atcgccgcgt
     6901 tcccggaggc gaaggagcgc gtgcccgacc tgcggatggt cgtcgtcgcg ggcccgcgga
     6961 tcgacccggc gtccctgccg gagcacgacg gcctcgaggt acgcgcctac gtccacgagc
     7021 tgtaccgcca cctcgccgtc tgcgacctcg cgatcgtcca gggcggcctg acgacggcga
     7081 tggagctgac ggcgaaccgc cggccgttcc tctacttccc gctccgccac cacttcgagc
     7141 agaacttcca cgtccgccac cggctcgagc gctacggcgc cggccggtgc atggacttcg
     7201 agacggacgg cccgccggag atcgccgccg cgatcgccga ggagatcggg cgcgaggtcg
     7261 actaccggcc ggtcgagacc gacggcgccg cgcgcgcggc gaggctcatc gccgagctgc
     7321 tctgacccgc gccggtgcgg ggccggaacg ccgaccccgc acgacggcgc gctaggagcc
     7381 ggagccggtc gccttccaga agtagtagtc cgcggtgtac ggcacctcgg cctgcgtccc
     7441 gaccgtcccg gcatggcacg aggcctccgc gggcgcgagc ccgccggtgg tggcgatccg
     7501 ctggatgaac gtcgtgtggg cgaggcggtc gccgtccgcg ccggccgagg tcgaggcggc
     7561 cgcgaggagg agccacggga tcgccccggc gacgggagcg cgatccacga ccgtgccgac
     7621 gacgaggctg ccgtcccgcg cctgccagga gggcccgccg aagtgcgtcg tgagaagcat
     7681 gccgcgctcg tcgtagaggt tcgccctcgg agcgacgaag tcccaccggt aaccgcccgc
     7741 aaccgcgttg cacgcgtaga tctggacgcc gacggcctcc ccgacgagga acaccttgtt
     7801 cccctcgggc accgcgatcg cctccgggac gtcgggcgct gcgggaccgg cgtatgcggc
     7861 ggcggcgagg acggccgccg cgatcacggc cacggacagg gcgcccacct tgatgaacgg
     7921 cttcatgcga gatacctccg ttggtcacgc gtccggcgga gatcgccgga cgtcgcgcgg
     7981 aggagacacg agcccggttg cgattcgcct gcgatccggt aaagacgggc cggcgacgcg
     8041 gctgctagcc gtccgcgtcc tcggcggcct gctcccggtc ctcctcggtg tagcaggcct
     8101 cggtgagcgc aacgagctcc gccgcgagga gcgcctgcgt gcgctcgtcg gcctcggcgc
     8161 tctcgcgccg gaacccgcgc gcgacctcgc cggtctcggc gtaccgctcg tagccgtcga
     8221 gcacctcctg ccgcaggctc tcgacctcgt cgagcgcgct cgtgaaccgc tcgacccccg
     8281 cctcgcattc ctcgcggtac tcgccgctca ggccctcgaa cgcggctcgg acctcatccg
     8341 tcgcgtcgat gagggctcgg ttctcctcca gatggcagcg cacggccctg cgcggcgagg
     8401 ctccctcgtc gaacacccgt tgcgcctcct cgttgcacgc ctcgccgcgc gcgcggtacg
     8461 agctccatgc cccgctgaag ctgccgagcg acgggttcag cgcctcgctc tcgaccgacg
     8521 tgaaataccc ctcgcgcggg tcggacgcct cggtcgtcgc cgccggcgcg gtcgccgccg
     8581 gctcggagtc gccgccgcac gccgagagca cggccgccgc cgtcacgacc acgagcatcc
     8641 tgaacaggat cacgccaccc ccaaatcgtc gcccgccacg ccgcccgtcc ggccgaaacc
     8701 cggcggacgg ccgcactcta gacgttgcgg cgaccgccga ccacgggtcc ggcgacccgg
     8761 gcggccggaa cccggcgcgc aactagcccg ccgcatcgcg cgggcggcgg gccgcccgga
     8821 cgcgtcgccg caggacgtgg tatccgtggc gcgcgccggt gcgggcgagc cggggatccg
     8881 cgacgaccgc gcgcgcgagc gcccgcccgc cccgctcgtg ggcctcgagc agccggtcga
     8941 gatcgcgaag ccagccgacc ctgtcctcga ggaggccgcg cagctccgcg tggccctgca
     9001 cgcccgggcg tgcgagcagg agggagtcgg acgcgagccg cccctggagg tgctcccggc
     9061 ggtcgcccgt gaccgagccg gcgcgccggc ggtagaggta gtacgcgtcc ggcacctgga
     9121 tccaccgcgc gccggcgagc gcgaggtcga cgaagaagca gaagtcggag ccgaccggca
     9181 gcgacccgtc gtagcggatg ccgtggtcgc gcacgaacgt ccgccgcacg agcggcttga
     9241 ggagccccag cccgtaccgg gcgaggtcgg cgggccgcag ctcgaacggc tccgccgccc
     9301 ggacgccgtg cgaggcgagc agccttcgcg tgcgcttccc ggcctcctcg atcacgatgt
     9361 cgtcggtcac gacgtccgcc tccgcgagga ggggcgtcag ccgctcgagc cgctcgggca
     9421 gccaggcgtc gtccgcgtcg agcaccgcga cccagtcgcc gcgcgccgcg tcgatcgc
//
LOCUS       DASTFC010000045         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1253381,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000045 DASTFC010000000
VERSION     DASTFC010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253381"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFO88_01310"
     CDS             <1..373
                     /locus_tag="VFO88_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9544193.1"
                     /translation="LSVYHELGLDYYSATSNTFIGKIYERLGLENIADDAEKAGTEYP
                     QLSVEHIVASNPDLIVLADTKCCGQTAETVAKRPGWQRIDAVRNGNVVEADDDITSRW
                     GPRTVDFVALVVDALAGLERR"
     gene            370..1449
                     /locus_tag="VFO88_01315"
     CDS             370..1449
                     /locus_tag="VFO88_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET9544194.1"
                     /translation="MTEAGLRARLARRADRPLPGISVAWALGVAGFVLLAALLGLALG
                     PVGLGTGRVARELLSHVPGLGVESPLTPTEDAILWELRAPRVALGLVVGALLASAGAA
                     YQGVFRNPLADPYLLGAAAGAGLGATLVIAYDVGDPLGVDLRPVAAFAGAILGVAAAY
                     GLGRSVGGGRSTATLILAGVTVAAFLTAVQTFLQQRNTETVQEVYAWILGQLETSGWS
                     DVAIALPYAIVSTALLVWHRRLLDVLAVGDDEAASLGVAVARVRLVVVAAATLGTAAA
                     VAFSGLIAFVGIIVPHAIRLLVGTSYRAVLPLSLGVGAGFLVLTDLICRTAIAPAELP
                     IGVVTAFLGAPFFAVVLRTTRTVGP"
     gene            1446..2243
                     /locus_tag="VFO88_01320"
     CDS             1446..2243
                     /locus_tag="VFO88_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9544195.1"
                     /translation="MSVVRARGVGVVLDGVRVVDGVGLELERGGWIALIGPNGAGKTT
                     FLRAVAGLVPYDGSLELLGSEVASLARRDVARRVAVVPQLPVIPPDMSVLEYVVLGRT
                     PHLGYAGAPGRRDVDAARAALGALDVLQLAGRRLGSLSGGERQRAVLARAVAQDGTLL
                     LLDEPTSALDVGAQQQVLELVASLRAERGLTVLAAMHDLTLAGQYADRLLLLDGGREA
                     ASGAPAEVLTEELVARHYGASVRVVEGEEGVAVVPLRPRVDAERPVG"
     gene            2243..2761
                     /gene="cobU"
                     /locus_tag="VFO88_01325"
     CDS             2243..2761
                     /gene="cobU"
                     /locus_tag="VFO88_01325"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET9544196.1"
                     /translation="MGLVFLLGGARSGKSALAVRLAAESDSPVVFVATTEVRGGDEEL
                     RARVDAHRAERPSSWATVEEPLELAHALREAPAEACVVVDCLTLWTANALEAGLDDAA
                     VERAAVEAADVAASREALTVVVSNEVGLGVVPATPLGRRFRDVLGRVNAVFAAAAERA
                     SFVVAGRELELR"
     gene            2758..>3075
                     /locus_tag="VFO88_01330"
     CDS             2758..>3075
                     /locus_tag="VFO88_01330"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET9544197.1"
                     /translation="MTSTVAFDLDALVARIRPVDEEARAAAQAALDAKTKPRGSLGAL
                     EELAVRVAGIRGDAAPGRVASAIVLAAADHGYARQGVSAYPPEVTGQMLANFLAGGAA
                     VNVL"
BASE COUNT          311 a         1144 c         1192 g          428 t
ORIGIN      
        1 cctgagcgtc taccacgagc tcggcctcga ctactactcg gccacctcga acacgttcat
       61 cgggaagatc tacgagcggc tcggcctcga gaacatcgcg gacgacgcgg agaaggccgg
      121 aaccgagtat ccgcagctct cggtcgagca catcgtcgcc tcgaaccccg acctgatcgt
      181 cctcgcggac acgaagtgct gcggacagac cgcggagacg gtcgcgaagc gcccgggctg
      241 gcagcggatc gacgcggtcc ggaacgggaa cgtcgtcgag gccgacgacg acatcacgtc
      301 gcgctggggc ccgcggacgg tcgacttcgt ggcgctcgtc gtcgacgcgc tcgcggggct
      361 ggagcgccgg tgaccgaggc cggtctccgc gcgcggctcg cacgccgggc cgaccggccg
      421 ctcccgggca tctcggtcgc ctgggcgctc ggcgtcgccg ggttcgttct tctcgccgcg
      481 ctgctcgggc tcgcactcgg gccggtcggc ctcggcaccg gccgggtggc ccgcgagctc
      541 ctgtcgcacg ttccgggcct cggcgtcgag tcgccgctca cgccgaccga ggacgcgatc
      601 ctctgggagc tccgggcgcc gcgcgtcgcg ctcgggctcg tggtcggcgc gctcctcgca
      661 tcggccggcg ccgcctacca gggggtcttc cggaacccgc tcgccgaccc gtacctcctc
      721 ggggcggctg cgggggcggg cctcggggcg acgctcgtga tcgcgtacga cgtcggcgac
      781 ccgctcggcg tcgacctgcg gccggtcgcc gcgttcgccg gcgcgatcct cggcgtcgcg
      841 gccgcgtacg ggctcggccg ctcggtcggg ggcgggcgga gcaccgcgac gctgatcctc
      901 gccggcgtca ccgtggccgc gttcctcacg gcggtgcaga ccttcctcca gcagcggaac
      961 acggagacgg tgcaggaggt ctacgcgtgg atcctcggcc agctcgagac ctccgggtgg
     1021 agcgacgtcg cgatcgcgct tccgtacgca atcgtctcga ccgcgctcct cgtctggcac
     1081 cggcggctgc tcgacgtgct cgccgtcggg gacgacgagg cggcctcgct cggcgtcgcg
     1141 gtcgcgcgcg tgcggctcgt cgtcgtggcg gccgcgacgc tcgggacggc ggccgcggtc
     1201 gccttcagcg gcctcatcgc gttcgtcggg atcatcgtcc cgcacgcgat ccggctcctc
     1261 gtcgggacga gctaccgcgc cgtgctcccg ctctcgctcg gcgtcggagc ggggttcctc
     1321 gtcctcacgg atctcatctg ccggacggcg atcgcgcccg cggagctccc gatcggcgtc
     1381 gtgaccgcgt tcctcggggc gccgttcttc gcggtcgtcc tgcgcacgac taggacggtc
     1441 gggccgtgag cgtcgttcgt gcgcgcgggg tcggcgtcgt gctcgacggc gtccgggtcg
     1501 tggacggggt cgggctcgag ctcgagcgcg gcgggtggat cgcgctcatc gggccgaacg
     1561 gcgccggcaa gacgaccttc ctccgcgcgg tcgcggggct cgtcccctac gacggctcgc
     1621 tcgagctgct cggttcggag gtcgcgtcgc tcgcgcggcg tgacgtcgcg cgccgcgtcg
     1681 ccgtcgtgcc ccagctcccg gtcatcccgc cggacatgag cgtcctcgag tacgtcgtgc
     1741 tcggccggac gccgcacctc ggctacgcgg gggcaccggg gcggcgcgac gtcgacgcgg
     1801 cccgcgcggc gctcggggcg ctcgacgtcc tccagctcgc cgggcggcgg ctcggctcgc
     1861 tctccggcgg ggagcggcag cgggccgtgc tcgcgcgcgc cgtggcgcag gacgggacgc
     1921 tgctcctcct agacgagccg acgagcgccc tcgacgtcgg cgcgcagcag caggtgctcg
     1981 agctcgttgc ctccctgcgg gccgagcgcg ggctcaccgt gctcgcggcc atgcacgacc
     2041 tgacgctcgc cgggcagtac gccgaccggc tgctcctcct cgacggcggc cgggaggcgg
     2101 cgagcggggc gcccgccgag gtgctgacgg aggagctcgt cgcgcgccac tacggcgcct
     2161 cggtgcgcgt ggtcgagggg gaggagggcg tggccgtcgt cccgctgcgg ccgcgcgtcg
     2221 acgccgagcg gccggtgggc tagtgggcct cgtgttcctc ctcggcggcg cgcggagcgg
     2281 gaagtcggcg ctcgccgtgc gtctcgcggc ggagtccgac tcgcccgtcg tgttcgttgc
     2341 gacgaccgag gtgcgcggcg gcgacgagga gctgcgcgcg cgcgtcgacg cgcaccgggc
     2401 cgagcggccg tcctcgtggg cgacggtcga ggagccgctc gagctcgcgc acgcgctgcg
     2461 cgaggcgccc gccgaggcgt gcgtcgtcgt cgactgcctc acgctctgga cggcgaacgc
     2521 gctcgaggcg ggcctggacg acgccgcggt cgagcgcgcg gccgtcgagg cggcggacgt
     2581 cgccgcgtcg cgcgaggcgc tcaccgtcgt cgtcagcaac gaggtggggc tcggcgtcgt
     2641 gccggcgacg ccgctcggcc ggcgcttccg cgacgtcctc ggccgcgtga acgcggtctt
     2701 cgccgcggcc gccgagcgtg cgtccttcgt cgtcgcgggg cgggagctcg agctccggtg
     2761 acgagcacgg tcgccttcga cctggacgcg ctcgtcgctc ggatccggcc cgtggacgaa
     2821 gaggcgcggg ccgcggcgca ggcggcgctc gacgcgaaga cgaagccgcg cgggagcctc
     2881 ggcgcgctcg aggagctcgc cgtgcgggtg gccgggatcc gcggcgatgc cgcgccggga
     2941 cgcgtcgcgt cggcgatcgt gctcgccgcc gccgaccacg gctacgcacg gcagggcgtg
     3001 agcgcgtacc cgcccgaggt gaccggccag atgctcgcca acttcctcgc cggcggcgcg
     3061 gcggtcaacg tcctc
//
LOCUS       DASTFC010000046         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_759023,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000046 DASTFC010000000
VERSION     DASTFC010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759023"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFO88_01335"
     CDS             <1..351
                     /locus_tag="VFO88_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9544198.1"
                     /translation="RGRLTGTRPATGQYLTVVSSGVPELLDTRGRDLVSLLAGEVTAR
                     LGPANVLWSRVSREPAATFLGRPGTEALRLGPETGLPGVVRAGAWTRTGWPATMEGAI
                     RSGRRAARLLIDGA"
     gene            443..2314
                     /gene="shc"
                     /locus_tag="VFO88_01340"
     CDS             443..2314
                     /gene="shc"
                     /locus_tag="VFO88_01340"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET9544199.1"
                     /translation="MPDGRPASVAPEVALDRAVRKATARLLDLQTPGGWWVGELESNV
                     TMTAQHVFLSHFLRRLDDDTLRRCANELLARQRPDGTWAIYHGGEPDLAATIEAYAAL
                     RLAGLGADDSRLAGARAYVESRGGIGASRVFTRIWLALLGLWSWDDVEQIPVELVLLP
                     SRGPLSVYDFACWARQTLVPLAVVLHYRPVRPVPEERACRELDLGPAPRGRGPLDLAD
                     RLLARYARARVKPGRARALALAERWILDRQELDGSWGGIQPPWVWSLVALVCRGHGPD
                     SPYLRRGIAGWRRFLVEDGDRLRPEACQSPVWDTGLAVLALRAAGVEPDHPALARAAG
                     WLLGEEVRARGDWALRRPGAEPGGWSFEHDNDLYPDVDDAAVVGLALHELGQGDAAVE
                     RATGWVEAMQSSGGGWGAFDVDNRAEWLYGLPFCDFGFVIDPPSVDVSAHALELLAAR
                     GGSEESLRRGVDYVLAEQEPDGSWFGRWGVNYVYGVGAAVPGLVAAGVPRGHPAIRRA
                     VAWLERCQNADGGFGEDCRSYDLDGTGDPWRGHGGSTASQTAWALLALVAAGEERSPV
                     AERAAAYLCASQREDGDWDEEAFTGTGFPRDFMIRYHLYRIVWPLLALGRLREASRA"
     gene            2311..>2552
                     /locus_tag="VFO88_01345"
     CDS             2311..>2552
                     /locus_tag="VFO88_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9544200.1"
                     /translation="MKVHVTGASGFVGSHVAAALRAHGADVRDERVELLDRPALERAF
                     AGCDAVVHVAALYSFDARAEELERVNVAGTENVLAA"
BASE COUNT          271 a          993 c          960 g          328 t
ORIGIN      
        1 cgcgggcgcc tgaccgggac gcggcccgcg accggccagt acctcaccgt cgtctcgagc
       61 ggcgtgccgg agctgctcga cacgcggggg cgcgacctcg tctcgctcct cgccggcgag
      121 gtgacggcgc ggctcgggcc cgcaaacgtc ctctggtcgc gggtcagccg ggagccggcc
      181 gcgaccttcc tcggccgccc cggcacggag gcgctgcggc tcggcccgga gaccggcctc
      241 ccaggtgtcg tccgcgccgg cgcgtggacg cggaccggct ggccggccac gatggagggc
      301 gcgatccgca gcggccgccg ggcggcgagg ctcctgatcg acggcgcgtg acgcccggcg
      361 ccgcgcgcga tacgctgaac acacggggca cggagcgtcc cggcctgcga tgagtgtggt
      421 cgaatcgcac aaggcagcca cggtgcccga cggtcgcccg gcgagcgtcg cgcccgaggt
      481 cgcgctcgac cgggccgtgc gcaaggcgac cgcgcggctg ctcgacctcc agacgcccgg
      541 cggctggtgg gtcggcgagc tcgagtcgaa cgtgaccatg acggcgcagc acgtcttcct
      601 ctcgcacttc ctgcgccggc tcgacgacga caccctgcgg cgctgcgcga acgagctcct
      661 cgcgcggcag cggccggacg gcacgtgggc gatctaccac ggcggcgagc ccgacctggc
      721 ggcgacgatc gaggcgtacg cggcgctccg tctcgccggc ctcggcgcgg acgactcccg
      781 cctcgcgggc gcgcgcgcct acgtcgagag ccgcggcggg atcggcgcct cgcgcgtctt
      841 cacccgcatc tggctcgcgc tcctcgggct ctggagctgg gacgacgtgg agcagatccc
      901 ggtcgagctc gtcctcctcc cgtcgcgggg cccgctctcc gtctacgact tcgcctgctg
      961 ggcgcggcag acgctcgtcc ccctcgccgt cgtcctccac taccggccag tccggcccgt
     1021 gccggaggag cgcgcctgcc gcgagctcga cctcggcccg gcaccgcgcg ggaggggccc
     1081 gctcgacctc gccgaccggc tcctcgcacg ctacgcccgg gcgcgtgtga agcccggccg
     1141 cgcgcgagcg ctggccctgg cggagcgctg gatcctcgac cggcaggagc tcgacggctc
     1201 gtggggcggg atccagccgc cgtgggtctg gtcgctcgtc gcgctcgtgt gccgcggcca
     1261 cgggcccgac tcgccgtacc tccgccgcgg gatcgcgggc tggcgccggt tcctcgtcga
     1321 ggacggcgac cgcctgcggc ccgaggcgtg ccagtcgccc gtgtgggaca cgggcctcgc
     1381 cgtcctcgcc ctccgcgcgg ccggcgtcga gcccgaccat ccggcgctcg cccgcgcggc
     1441 cggctggctg ctcggggagg aggtgcgcgc gcgcggcgac tgggcgctcc gccggcccgg
     1501 cgccgagccg ggcggctggt cgttcgagca cgacaacgac ctctatccgg acgtcgacga
     1561 cgcagccgtc gtcggcctcg ccctccacga gctcgggcag ggcgacgcgg cggtcgagcg
     1621 cgcgaccggc tgggtcgagg cgatgcagtc gtccgggggc ggctggggcg ccttcgacgt
     1681 ggacaaccgg gccgagtggc tctacgggct cccgttctgc gacttcggct tcgtcatcga
     1741 cccgccgagt gtcgacgtga gcgcccatgc gctcgagctc ctcgccgcgc ggggcgggag
     1801 cgaggagtcg ctccgccgcg gcgtcgacta cgtcctcgcc gagcaggagc ccgacggctc
     1861 gtggttcggc cgctggggcg tgaactacgt ctacggcgtc ggcgccgccg tccccggcct
     1921 cgtggccgcc ggggtacccc gcgggcaccc cgcgattcgc cgtgccgttg cgtggctcga
     1981 acgctgtcag aatgcggacg gcggcttcgg agaggactgc cgctcgtacg acctcgacgg
     2041 caccggcgac ccctggcgcg gccacggcgg gtcgaccgcc tcgcagaccg cgtgggcgct
     2101 tctcgcgctc gtcgccgccg gggaggagcg ctcgcccgtc gccgagcgtg ccgccgccta
     2161 cctctgcgcg agccagcgcg aggacggcga ctgggacgag gaggcgttca ccggcaccgg
     2221 cttcccgcgc gacttcatga tccgctacca cctctaccgc atcgtctggc cgctcctcgc
     2281 gctcggccgc ctcagggagg cctcgcgcgc gtgaaggtcc acgtcaccgg cgcgagcggg
     2341 ttcgtgggga gccacgtcgc cgctgcgctc cgcgcgcacg gggcggacgt gcgcgacgag
     2401 cgcgtcgagc tcctcgaccg tcccgcgctc gagcgggcgt tcgccggctg cgacgccgtc
     2461 gtccacgtcg ccgcgctcta cagcttcgac gcgcgcgcgg aggagctcga gcgcgtgaac
     2521 gtcgccggga cggagaacgt cctcgccgcc tg
//
LOCUS       DASTFC010000047         4891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_693075,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000047 DASTFC010000000
VERSION     DASTFC010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693075"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFO88_01350"
     CDS             complement(<1..542)
                     /locus_tag="VFO88_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9544201.1"
                     /translation="MLETDVVVVGAGIAGLGAATALAQEGRNVVVLEARDRVGGRTEN
                     VEIGGQPNEIGGQWVAPYQSELEGLLEELGIELFPSHRTGEHVYVGPDKRVTRYEGHD
                     APLSDAAGRSYADAAAKLDELAAQLDPDAPWEHPDALALDALTYEEWLRREVEDDAAR
                     DLLRSFLAGGFMTKPAQSFSL"
     gene            complement(574..1374)
                     /locus_tag="VFO88_01355"
     CDS             complement(574..1374)
                     /locus_tag="VFO88_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HET9544202.1"
                     /translation="MRTGTAGRLPGEALCGHAVRIYHEDAELVACVASYLAAGLAAGE
                     PAVAVVTREHRAGLAAALARLGHDPLALEAAGLLTTADAEATLACFLEGDGPSAGRFH
                     TVIGALLAEAQRGRPGKPVRVFGEMVDLLARRGRHDAALAVEELWDGLVGRRGISLLC
                     AYRLDLFDAETQRGVLPRVYRAHDHVEPALDPARLEHAVAHALDEALGREEAERVRRL
                     AVDARRDAHVPAAQLALLWVSERMPVAAGRILAAARAAYERPAPAGAR"
     gene            1439..2122
                     /locus_tag="VFO88_01360"
     CDS             1439..2122
                     /locus_tag="VFO88_01360"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9544203.1"
                     /translation="MAEAPALVETVVEIGGREVSLLHPPDAEALLSEEAFEREEFLPY
                     WAELWPSGLALAREVAGRPLAGARVLELGCGLGLPSLAAALGGADVLATDWARDAIAL
                     LDRNAERNGIRLETAAASWTAPEPLVERGPWDLVLAADVLYERRDAEPLLDLLPRLVT
                     RDEAPASPLPSNTVLQARGSRRPGEILLAEPGRPPAAAFLAAARERWQVTTTPLQEHP
                     RVDLHAIGV"
     gene            2155..2838
                     /locus_tag="VFO88_01365"
     CDS             2155..2838
                     /locus_tag="VFO88_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9544204.1"
                     /translation="MRAAIVLLEEEPRFRPYPFGLTLTGALQRAVGDARDTLARTPGR
                     FDGPIAFCHGVTPEGVIVRRAGRYAEAMVVFQEPSLAYKLGFTLGVQLFVERPEGAVL
                     FQLRGPSIGRDPLLWTASASGGVMPGEEPRAAVLADAAEEIALGPDDLPGFRPVAVCV
                     NDDSGSALVVYHAMLREGAEPLPNEAKVAELHWAAEPGAIGAQVSGDTLASWQALDAW
                     RAAHAPREA"
     gene            2910..4502
                     /locus_tag="VFO88_01370"
     CDS             2910..4502
                     /locus_tag="VFO88_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544205.1"
                     /translation="MAKEKPAATDTEKGFGTGLRAQLERRRAALLEAQAESGAEQAEA
                     AAKVAAEQNGGATADIESVREELAASLAREEGLRATLSEEIAASERGLELDEKLAARA
                     AELDGREAELAQLRERLDSDRIELERTQEALVKAQAQLADAGDESGERERALEETIAA
                     LEAREQQHEAKITELEQREQEREAKLAELESRERDRETSQAGEAELNELQAELRKREQ
                     AVQSAEKALAAREAAAEAATADVARRETALNEREAGLVAHEAEVAKRKRELDAERKQK
                     AGDVRKEMEAREKILAAREQAIAEREQAIEAEAASRAAQLEERKEALRVEAERLEAEL
                     EAKREDLAGREEAMRADAARLETELSERTRAADEAMTRLTEIQARETELAVHEAELHR
                     REAALSRVETAALKSGELEELEGRLHARERELAEREAAFQNAEQRADAERGRLAKREK
                     RTAELEESLHGRIRELDEREAAVDEREARVQADHDIREDKLERWQAELSDLERRLQHK
                     ETELAEYVAQVQGALNKRQAVG"
     gene            complement(4565..>4891)
                     /locus_tag="VFO88_01375"
     CDS             complement(4565..>4891)
                     /locus_tag="VFO88_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544206.1"
                     /translation="FASVYADRYYQIVKDPPGPDDAEAEVIASGRAKRSAIGVREGAT
                     ALELTCRETGSEAASLTLTANGVELARVRDRDPLLYVTGVGLFVASTSGDAGAAFDDL
                     RVEVLD"
BASE COUNT          673 a         1812 c         1885 g          521 t
ORIGIN      
        1 agcgagaacg actgcgccgg cttcgtcatg aacccgcccg cgaggaagga gcggagcagg
       61 tcgcgggccg catcgtcctc cacctcgcgg cgaagccact cctcatacgt gagggcgtcg
      121 agcgcgaggg cgtccgggtg ctcccacggc gcgtccggat cgagctgcgc ggcgagctcg
      181 tcgagcttcg ccgccgcgtc cgcgtacgag cggccggccg cgtcggagag cggcgcgtcg
      241 tgcccctcgt agcgcgtgac ccgcttgtcc gggccgacgt agacgtgctc gccggtccgg
      301 tggctcggga agagctcgat cccgagctcc tcgaggaggc cctcgagctc cgactggtac
      361 ggcgcgaccc actggccgcc gatctcgttc ggctggcccc cgatctcgac gttctctgtc
      421 ctcccgccga cgcggtcgcg cgcctcgagc acgacgacgt tgcgaccctc ctgcgcgagc
      481 gcggtcgcgg cgccgagccc cgcgatcccc gcgccgacca cgacgacgtc cgtctcgagc
      541 atgggcggac cctaccgcgg agcccgcgcg cgttcagcgg gcgccggcgg gcgcgggccg
      601 ctcgtaggcg gcgcgcgcgg cggcgaggat gcgcccggcg gcgaccggca tccgctcgct
      661 gacccacagg agcgcgagct gcgcggccgg gacgtgcgcg tcgcggcgcg cgtcgaccgc
      721 gaggcggcgc acgcgctccg cctcctcccg cccgagggcc tcgtcgagcg cgtgggcgac
      781 ggcgtgctcg agccgagccg ggtcgagcgc cggctccacg tgatcatggg cgcggtagac
      841 gcgcgggagg acgccgcgct gcgtctccgc gtcgaagaga tcgagccggt acgcgcagag
      901 gagcgagatc ccgcggcggc cgacgagacc gtcccagagc tcctcgacgg cgagagccgc
      961 gtcgtgccgc ccccggcggg cgaggaggtc gaccatctcg ccgaagacgc gcacgggctt
     1021 cccgggcctg ccgcgctgcg cctcggcgag cagcgcgccg atcaccgtgt ggaagcgccc
     1081 ggcggacggc ccgtctccct cgaggaagca ggcgagcgtc gcctcggcgt cggcggtcgt
     1141 gaggaggccg gccgcctcga gcgcgagcgg gtcgtgcccg aggcgtgcga gcgcggcggc
     1201 gagcccggcc cggtgctccc gggtgacgac ggcgacggcc ggctcgcccg ccgcgaggcc
     1261 cgcggccagg tacgaggcga cgcaggcgac gagctcggcg tcctcgtggt agatccgaac
     1321 ggcgtggccg cacagggcct cgccggggag acgccccgcc gttccagtgc gcatcgtgcg
     1381 gcatcgtaac ggtcgaatgc gcgcttcgcg gcgtggtttc ccggctacgc tgcgcatcgt
     1441 ggccgaggcg cccgcgctcg tcgagacggt cgtcgagatc ggcggccggg aggtctcgct
     1501 gctccacccg cccgacgcgg aggcgctcct ctcggaggag gcgttcgagc gggaggagtt
     1561 cctgccctac tgggccgagc tctggccgag cgggctcgcg ctcgcgcggg aggtggcggg
     1621 ccggccgctc gcgggcgccc gcgtgctcga gctcggctgc gggctcgggc tcccgagcct
     1681 cgccgccgcc ctcggcggcg cggacgtcct cgcgaccgac tgggcacgcg acgcgatcgc
     1741 gctgctcgac cggaacgccg agcggaacgg gatccgcctg gagaccgccg ccgcctcgtg
     1801 gacggcgccg gagccgctcg tcgagcgcgg gccgtgggat ctcgtcctcg ccgccgacgt
     1861 cctatacgag cggcgagacg cggagccact cctcgacctg ctcccccggc tcgtcacgcg
     1921 ggacgaggcg cccgcgagcc ccctcccaag taacactgtg ttacaagcga ggggctcccg
     1981 ccggcccggg gagatcctcc tcgccgagcc cggccggccg ccggcggcgg cgttcctcgc
     2041 ggcggcacgg gagcggtggc aggtgacgac gacgcccctc caggagcacc cgcgcgtcga
     2101 cctccacgcg atcggcgtct gacggcagcc gggtcgatcg gccacaatca ggggatgcgc
     2161 gccgccatcg tcctgctcga ggaggagccc cgcttccgcc cgtatccctt cggcctgacg
     2221 ctgaccgggg cgctgcagcg cgcggtcggc gacgcgcgcg acacgctcgc ccgcacgccg
     2281 ggacgcttcg acgggccgat cgcgttctgc cacggcgtca cgccggaggg cgtgatcgtg
     2341 cgccgcgccg gccgctacgc ggaggccatg gtcgtcttcc aggagccctc gctcgcctac
     2401 aagctcgggt tcacgctcgg cgtgcagctc ttcgtcgagc ggcccgaggg agccgtgctc
     2461 ttccagctcc gcgggccctc gatcggacgt gacccgctgc tctggacggc ctccgcgtcc
     2521 ggcggcgtca tgccgggtga ggagccgcgg gccgcggtgc tcgccgacgc ggcggaggag
     2581 atcgccctcg ggcccgacga cctgccgggt ttccggccgg tcgcggtctg cgtgaacgac
     2641 gactccggct ccgcgctcgt cgtctaccac gcgatgctgc gcgagggcgc ggagccgctc
     2701 ccgaacgagg cgaaggtggc cgagctccac tgggcggcgg agccgggcgc gatcggcgcg
     2761 caggtctccg gcgacacgct cgcctcctgg caggcgctcg acgcctggcg cgccgcgcac
     2821 gcccccagag aggcctgatc gccaccaacc gataggggga tgcggcaccc ccccgaacgc
     2881 gccgatgcca tcggtgaccc gcctgccgca tggccaaaga gaaacccgcc gccaccgaca
     2941 ccgagaaggg cttcggaacc gggcttcgcg cacagctcga gcgtcgccgt gccgcgctgc
     3001 tcgaggccca ggccgagtcc ggtgccgagc aggccgaggc ggccgccaag gtcgcggccg
     3061 agcagaacgg cggcgccacc gccgacatcg agtccgtccg cgaggagctc gcggcgtcac
     3121 tcgcgcgtga ggagggtctc cgcgcgacgc tgagcgagga gatcgcagcc tccgagcgtg
     3181 ggctcgagct cgacgagaag ctggcggcac gcgccgccga gctggacggc cgcgaggccg
     3241 agctggcgca gctccgcgag cggctcgact ccgaccgcat cgagcttgag cgcacgcagg
     3301 aggcgctcgt caaggcgcag gcgcagctcg cggacgcggg cgacgagagc ggcgagcgcg
     3361 agcgcgcgct cgaagagacg atcgcggcgc tcgaagcccg cgagcagcag cacgaggcga
     3421 agatcacgga gctggagcag cgcgagcagg aacgcgaggc gaagctggcg gagctcgaga
     3481 gccgcgagcg cgaccgcgag acgtcgcagg ccggcgaggc cgagctgaac gagctccagg
     3541 ccgagctccg caagcgcgag caggccgtcc agtcggccga gaaggccctg gccgcccgcg
     3601 aggccgccgc cgaggccgcg accgccgacg tcgcgcgccg cgagaccgcg ctgaacgagc
     3661 gcgaggccgg cctcgtcgcc cacgaggcgg aggtcgcgaa gcgcaagcgg gagctcgacg
     3721 ccgagcggaa gcagaaggcg ggcgacgtcc gcaaggagat ggaggcgcgc gagaagatcc
     3781 tcgccgcccg cgagcaggcg atcgcggagc gcgagcaggc gatcgaggcc gaggcggcct
     3841 cgcgcgcggc gcagctcgag gagcgcaagg aggcgctgcg cgtcgaggcg gagcggctcg
     3901 aggccgagct cgaggcgaag cgcgaggatc tcgccggccg cgaggaggcc atgcgcgccg
     3961 acgcggcgcg gctcgagaca gagctctccg agcgcacccg cgcggccgac gaggcgatga
     4021 cccgcctcac ggagatccag gcgcgcgaga ccgagctcgc cgtccacgag gccgagctcc
     4081 accgccgcga ggcggcgctc agccgggtcg agaccgcggc gctcaagtcg ggcgagctcg
     4141 aggagctcga ggggcgcctc cacgcgcgtg agcgggagct ggccgagcgc gaggcggcct
     4201 tccagaacgc tgagcagcgc gccgacgccg agcggggccg gctcgcgaag cgcgagaagc
     4261 ggaccgccga gctcgaggag agcctccacg ggcgcatccg cgagctcgac gagcgcgagg
     4321 cggcggtcga cgagcgcgag gcgcgcgtcc aggccgacca cgacatccgc gaggacaagc
     4381 tcgagcgctg gcaggccgag ctctcggacc tcgagcgccg gctccagcac aaggagacgg
     4441 agctcgccga gtacgtcgcc caggtgcagg gcgcgctgaa caagcgccag gccgtcgggt
     4501 agccgtcccc tccggccacg gccggtgcct ggcaccggac acgtccgcgg ggacgatcgt
     4561 cgggctagtc gaggacctcg acgcgaaggt cgtcgaaggc agcgcccgcg tcgccgctcg
     4621 tcgaggcgac gaagagcccg acgcccgtca cgtagagcag ggggtcccgg tcgcggaccc
     4681 gggcgagctc gacgccgttc gcggtcagcg tgagcgacgc cgcctcggag ccggtctccc
     4741 tgcaggtgag ctcgagcgcg gtggcgccct cccggacgcc gatcgcgctg cgcttcgcgc
     4801 gcccgctcgc gatcacctcg gcctcggcgt cgtccggccc gggcgggtcc ttcacgatct
     4861 ggtagtagcg gtccgcgtac acggacgcga a
//
LOCUS       DASTFC010000048         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1550078,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000048 DASTFC010000000
VERSION     DASTFC010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550078"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFO88_01380"
     CDS             <1..198
                     /locus_tag="VFO88_01380"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET9544207.1"
                     /translation="AIVRLVVLALLVLYALVFVLMNRDDVEVSFVFFTATTPLIVALV
                     LVGVIGAVAGWLARGQARRRV"
     gene            complement(195..947)
                     /locus_tag="VFO88_01385"
     CDS             complement(195..947)
                     /locus_tag="VFO88_01385"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9544208.1"
                     /translation="MTRLDGKRALVTGAASGIGKATAERLAEEGASVLVTDIQDEAGE
                     AVAAGIRERGGHAVFLHHDVSSEGDWSAAVERATGELGGLDILVNNAGIGDLNPIEDT
                     SKEDYDRTIAITQTSVFLGMKHAGDALKASGSGSVVNVSSIFGASGGFGVSPAYHAAK
                     GAVRILTKNVALRWATEGVRVNSVHPGFIETPIIEQAMGTPVEEAMLETTPMGRLGRP
                     EEIAAVIVFLAGDDASFMTGSEVYVDGGYLAR"
     gene            1064..2128
                     /locus_tag="VFO88_01390"
     CDS             1064..2128
                     /locus_tag="VFO88_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544209.1"
                     /translation="MLNLPALDVAVGLVFLYVVLSLVCSTVNEIVATSVGLRARFLQM
                     GILNLLSAAPRPTRAGVETAKAFYGHPLVQGLIRPAHGPDPSLDPTSTARWWRKPPYP
                     SYIPSRTFVAALGDLLEDAERRIASVDEEEAKAVEARLQGAAAELEQKIAAVPNARLS
                     EALLSLYRAAGADAAAFKHATEEWFDDAMERVSGWYRRRIQVILAVIATLVVLLLNAD
                     TLASGRVLWRDDAVRASVVRQAEASAEAGRDAAEVDDAVQRLDLPLGWDLSFGDRPTQ
                     VPDDPLGWIAKLVGLALTIGAVLLGAPFWFDLLSKIVRVRATGAPPPATDAVRTGEGD
                     QKRAGPGAATPAGARASPRR"
     gene            complement(2135..3061)
                     /locus_tag="VFO88_01395"
     CDS             complement(2135..3061)
                     /locus_tag="VFO88_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9544210.1"
                     /translation="MAASGGTRAVVVALLANIGIAIAKFGGYLLTGSSSMLAESVHSV
                     ADSSNQALLLIAGKRATRRASEVHQFGYGRVRYVAAFVVSIVLFTLGGLFALYEAWHK
                     YSDPHPIDSWQWVPIAVLLIAVAMESFALRTAVIEANHARGKMSLRQYVRQSRSPEIP
                     VILLEDTGALCGLVFALFGVSMTLATGDGRWDAVGSAAIGVLLVVIAVFLAIEMHSML
                     IGESALPAHHDAIEASLCGEGIDRVIHMRTMHLGPDDVLVAAKVAVTATDSAEEVAAA
                     INAAEARVRAAVPLRLQVYIEPDLFRKDGTAP"
     gene            complement(3073..3915)
                     /locus_tag="VFO88_01400"
     CDS             complement(3073..3915)
                     /locus_tag="VFO88_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET9544211.1"
                     /translation="MNPLRRLVDSPAFVGTVVAVILANAVVLGLQTYPGLEERHGDTL
                     HLLNGIFLGFFVVEILLRVAAYLPRPWEFFRSGWNVFDFVAVGLAFAPGLRENATVLR
                     LARLARIVRVVHLLPDVRILITGVLRSLPPLGSMAILTTLILFVYGMVGWQLFASELP
                     DQWGTIGLAMLTLFVMLTLENFPAYMDAGMDVHPWSWIYFVTFVLVAAFVVLNVLIGI
                     VLNSMEEARELERRKRIVPGEGGIFDVAPAPVVERIAILRAALDELEQELAGAGRRRD
                     GDRE"
     gene            complement(3956..>4199)
                     /locus_tag="VFO88_01405"
     CDS             complement(3956..>4199)
                     /locus_tag="VFO88_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9544212.1"
                     /translation="AYVAWTKALEAIGPARASVWANAVPPLAVVIAAVTLGETITPWF
                     AAGAALVIGGVVLAQRSGAPARRAVRTRPVLAPAAR"
BASE COUNT          589 a         1518 c         1507 g          585 t
ORIGIN      
        1 gcgatcgtcc gcctcgtcgt cctcgcgctg ctcgtcctct acgccctcgt cttcgtcctc
       61 atgaaccgcg acgacgtgga ggtgagcttc gtcttcttca ccgcgacgac gccgctcatc
      121 gtcgcgctcg tcctcgtcgg cgtgatcggc gcggtcgccg gctggctcgc gcggggccag
      181 gcgcgccgcc gcgtctagcg cgcgagatat ccgccgtcca cgtagacctc cgagccggtc
      241 atgaaggacg cgtcgtcgcc cgcgaggaac acgatcacgg cggcgatctc ctccggccgg
      301 cccaggcggc ccatcggcgt cgtctcgagc atcgcctcct cgaccggggt gcccatcgcc
      361 tgctcgatga tcggagtctc gatgaacccc gggtgcaccg agttgacgcg cacgccctcc
      421 gtcgcccacc ggagcgcgac gttcttcgtg aggatccgga cggcgccctt cgcggcgtgg
      481 taggccggtg agacgccgaa gccgccgctc gcgccgaaga tcgagctgac gttcacgacg
      541 gacccgctgc cggaggcctt gagcgcgtcg cccgcgtgct tcatgccgag gaagacgctc
      601 gtctgcgtga tcgcgatcgt ccggtcgtag tcctccttcg aggtgtcctc gatcgggttc
      661 aggtcgccga tcccggcgtt gttcacgagg atgtccagcc cgccgagctc gcccgtggcg
      721 cgctcgaccg cggcgctcca gtccccttcc gaggacacgt cgtggtggag gaagaccgcg
      781 tgcccaccgc gctcgcggat gccggccgcg accgcctcgc cggcctcgtc ctgaatgtcc
      841 gtcacgagca cggatgcgcc ctcctcggcg agccgctcgg cggtcgcctt gccgatcccg
      901 ctcgccgcgc ccgtcacgag cgcccttttc ccgtccagcc tcgtcatgtg gctccttcct
      961 gtcagggttt ccggggtcga gtctccgctt tccggctgcg ccgaggcgca accgccggag
     1021 ccggcgggcg atttcgtccg gcccgcggcg taccgtcggt ttcgtgctga acctccccgc
     1081 gctcgacgtc gccgtcggcc tcgtcttcct ctacgtcgtc ctctcgctcg tctgctcgac
     1141 cgtgaacgag atcgtcgcga cctcggtggg gctgcgcgcg cggttcctcc agatggggat
     1201 cctgaacctg ctgagcgcgg cgccgcggcc gacgcgcgcc ggggtcgaga cggcgaaggc
     1261 gttctacggc cacccgctcg tgcaggggct gatccgcccg gcgcacggcc ccgacccctc
     1321 gctcgacccg acgagcacgg cgcgctggtg gcgcaagccg ccgtacccgt cgtacatccc
     1381 gtcgcggacc ttcgtcgcgg cgctcggcga tctcctcgag gatgccgagc ggcggatcgc
     1441 ctcggtcgac gaggaggagg cgaaggcggt cgaggcgcgc ctgcagggcg cggctgcgga
     1501 gctcgagcag aagatcgcgg cggttccgaa cgcgcggctc tcggaggcgc tgctctcgct
     1561 ctaccgcgcg gcgggggcgg acgcggccgc gttcaagcac gcgacggagg agtggttcga
     1621 cgacgcgatg gaacgcgtgt ccggctggta ccggcggcgg atccaggtga tcctggccgt
     1681 gatcgcgacg ctcgtcgttc tcctcctgaa cgcggacacg ctcgcctccg ggcgcgtgct
     1741 ctggcgcgac gacgcggtcc gggcgtccgt cgtgcggcag gccgaggcat ccgccgaggc
     1801 gggccgcgac gcggcggagg tggacgacgc ggtgcagcgc ctcgacctcc cgctcggctg
     1861 ggatctctcg ttcggcgacc ggccgacgca ggtgccggac gacccgctcg gctggatcgc
     1921 gaagctcgtc gggctcgctc tcacgatcgg agcggtgctg ctcggcgcgc cgttctggtt
     1981 cgacctcctg agcaagatcg tccgcgtccg cgcgaccggc gcgccgccgc ccgcaacgga
     2041 cgcggtccgc accggcgagg gcgaccagaa gcgcgcgggc ccgggtgccg ccacgccggc
     2101 cggcgcgcgc gcgagcccgc gccgctgagc cgggctacgg ggccgtcccg tccttccgga
     2161 agaggtccgg ctcgatgtag acctgcaggc ggagcggcac cgcagcgcgc acgcgcgcct
     2221 cggccgcgtt gatcgcggca gcgacctcct cggccgagtc ggtcgcggtg acggccacct
     2281 tcgcggcgac gaggacgtcg tcggggccga ggtgcatcgt ccgcatgtgg atgacgcggt
     2341 cgatcccctc gccgcagagc gaggcctcga tcgcgtcgtg gtgtgcgggg agcgccgact
     2401 cgccgatcag catcgagtgc atctcgatcg cgaggaacac ggcgatgacg acgagcagca
     2461 cgccgatcgc cgccgatccg accgcgtccc agcgcccgtc gccggtcgcg agcgtcatcg
     2521 agaccccgaa gagcgcgaac acgagcccgc agagtgcccc ggtgtcctcg aggaggatga
     2581 cggggatctc cggcgagcgg gactggcgaa cgtactgccg gagcgacatc ttcccgcgcg
     2641 cgtggttcgc ctcgatcaca gcggtccgaa gcgcgaagct ctccatcgcg accgcaatca
     2701 ggagcacggc gatcggcacc cactgccacg agtcgatcgg gtgcgggtcg ctgtacttgt
     2761 gccacgcctc gtagagcgcg aagagcccgc cgagcgtgaa gaggacgatc gagacgacga
     2821 acgcggccac gtagcgcacg cgcccgtagc cgaactggtg cacctcggac gccctgcgcg
     2881 tcgcgcgctt cccggcgatg agcagaaggg cctggttcga gctgtcggcg accgagtgca
     2941 cgctctcggc gagcatcgag ctcgaccctg tgaggaggta cccgccgaac ttcgcgatcg
     3001 cgatcccgat gttcgccaag agcgcgacga cgacggcgcg cgttccgccc gatgctgcca
     3061 cggcccggaa ggctactcgc ggtcgccgtc gcgccgccgg cccgccccgg cgagctcctg
     3121 ctcgagctcg tcgagcgccg cgcggaggat cgcgatccgc tcgacgacgg gcgccggcgc
     3181 gacgtcgaag atcccaccct cgccggggac gatccgcttg cgccgctcga gctcgcgcgc
     3241 ctcctccatc gagttgagga cgatcccgat gagcacgttc agcacgacga acgccgcgac
     3301 gagcacgaag gtcacgaagt agatccacga ccacgggtgg acgtccatcc ccgcgtccat
     3361 gtacgccggg aagttctcga gggtcagcat cacgaagagc gtgagcatcg cgaggccgat
     3421 cgtcccccac tggtccggca gctcggacgc gaagagctgc cagcccacca tcccgtagac
     3481 gaagaggatg agcgtcgtga ggatcgccat gctgccgagc ggcggcaggc tgcgcaggac
     3541 gcctgtgatg aggatccgga cgtcggggag caggtggacg acccggacga tccgcgcgag
     3601 gcgggcgagc cgcaggacgg tcgcgttctc ccgcagcccc ggcgcgaacg cgagcccgac
     3661 ggcgacgaag tcgaagacgt tccagccgct ccggaagaac tcccacgggc gcgggaggta
     3721 ggcggccacc cggagcagga tctcgacgac gaagaagccg aggaagatcc cgttcagaag
     3781 gtgcagcgtg tcgccgtggc gctcctcgag ccccgggtac gtctgcaggc cgagcacgac
     3841 cgcgttcgcg aggatgacgg cgacgacggt gccgacgaag gcgggtgagt cgacgagacg
     3901 gcgcagcggg ttcatgggcg cgccgaaatg tacggtctgc gggtgtcgcc gcggctcagc
     3961 gggcggcggg ggcgagcacc gggcgcgtgc ggaccgcgcg gcgggccgga gcgcccgagc
     4021 gctgcgcgag cacgacgccg ccgatcacga gcgccgctcc ggcggcgaac cagggcgtga
     4081 tcgtctcgcc gagcgtcacc gccgcgatca cgacggcgag cggcggcacg gcgttcgccc
     4141 agacggaggc gcgcgccggg ccgatcgcct cgagcgcctt cgtccacgcg acgtaggcg
//
LOCUS       DASTFC010000049         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1566525,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000049 DASTFC010000000
VERSION     DASTFC010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566525"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFO88_01410"
     CDS             <1..717
                     /locus_tag="VFO88_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9544213.1"
                     /translation="AWAAARLGLRAVVYAPEGASAAKLAHIERLGAETRLVGADLDAA
                     KDEARAFAEVEGLPFFEDGAEREQYDGYGAIGREIVEQLGGEPAAVVVPVGNGALLGG
                     IGIELCKSCPSTERIGVASTAAPVMIESWEAGRVVESDRSATFADGLAVRVAIPVAVA
                     VLGEVASRMVGVTEREIARAVGAFAAAGIRVEGAAAAGLAALPHLEDVDDPVVLVVTG
                     RNIDDELYARAVERPETFPE"
     gene            complement(953..1822)
                     /gene="dprA"
                     /locus_tag="VFO88_01415"
     CDS             complement(953..1822)
                     /gene="dprA"
                     /locus_tag="VFO88_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9544214.1"
                     /translation="MTYGVLRRGGAGYPSLLAQLHDPPARLHLRGDPDGIFERPAVAV
                     VGARSCSAYGAQVARTVARELAAAGVVVVSGMARGVDAEAHRGALEAGGTTLAVLGCG
                     IDRDYPARHAELARRIVSGAGLVVSEYEPGVEPAPWRFPARNRVIAGLSHATVVVEAR
                     ERSGALITADFALELGREVFAVPGEITTALSVGTNRLIRQGAAPLLGTDDVFAALGLE
                     AAAAPTVELTDAAARVLDLLADEPRGHDELVRASGIAASAVAAALVELELAGLTAEAD
                     GVHRVTRIPGRAA"
     gene            complement(1819..>2914)
                     /locus_tag="VFO88_01420"
     CDS             complement(1819..>2914)
                     /locus_tag="VFO88_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544215.1"
                     /translation="VAAEAARRSGLAKLLCAAESAPEAALAGIEPIPVRHLAEAGAYL
                     RGRHEPEPVGEPPAYAGPPPPDLREVRGQERARRALEIAAAGRHNLLLGGPPGTGKTM
                     LARRLPGILPPLTREEALEVTRIHSVAGALPAGQPLVAHPPFRAPHHTASTPAVVGGG
                     PGPRPGEATLAHRGVLLLDELGEFPRPSLEALRQPLEDGMVAIARIGGRHVFPARFLL
                     VGTMNLCPCGARGDPGAECSCSPARLAAYRQKLSRALLDRFDLVVTVPRARSQEVAGA
                     PGEPSSAVADRVIDADRRLRERPPPIGEAAGTVLARAVDRLGLSARGHARIWGVAVTI
                     GALAGVDEVCPEHMAEALSYRSPSELAVAA"
BASE COUNT          366 a         1085 c         1132 g          331 t
ORIGIN      
        1 gcctgggcgg ccgcgcggct cgggcttcgg gcggtcgtct acgcgcccga gggcgcgtct
       61 gccgccaagc tcgcgcacat cgagcggctc ggggcggaga cgaggctcgt cggcgccgat
      121 ctcgacgcgg cgaaggacga ggcgcgcgcg ttcgcggagg tcgaggggct tccgttcttc
      181 gaggacggcg ccgagcgcga gcagtacgac gggtacggcg cgatcggccg ggagatcgtc
      241 gagcagctcg gcggggagcc ggccgccgtc gtcgttcccg tcgggaacgg cgcgctgctc
      301 ggcgggatcg ggatcgagct ctgcaagagc tgcccgagca cggagcggat cggcgtcgcc
      361 tccaccgccg ccccggtgat gatcgagtcc tgggaggccg gccgagtcgt cgagtcggac
      421 cggagcgcca cctttgcgga cgggctcgcc gtccgtgtgg cgattcccgt cgcggtcgcg
      481 gtgctcggcg aggtggcgag ccggatggtc ggcgtcacgg agcgcgagat cgcgcgcgcc
      541 gtcggcgcct tcgcggcggc ggggatccgg gtcgagggcg ccgcagccgc cggccttgcg
      601 gcgctgccgc acctggagga cgtcgacgac ccggtcgtcc tcgtcgtcac cgggcggaac
      661 atcgacgacg agctgtacgc ccgtgccgtc gagcggccgg agacgtttcc cgagtgagcc
      721 ggcgccgcgc ggccgtctcc tgcggcggcg agtacgcggg cgcacgcgag gcccgtgacg
      781 cgccgcccgc gacggcgacc tccaccgtct cgtcggcgtc aagaggtgga tacgcctcac
      841 cgggcgggcc gagccagtaa gggacggtca cgtccgcact caaggcacgg cagcgcagct
      901 cgttattcga atgtcgctca cgaacgccgg ccgtgcggag gcgcgcgcgc cctcatgctg
      961 cgcgccccgg aatccgagtg acccggtgga cgccgtcggc ttcggcggtc agcccggcca
     1021 gctccagctc gacgagcgcg gccgcgacgg ccgaggcggc gatgccgctc gcacggacca
     1081 gctcgtcgtg gccgcgaggt tcgtccgcga gcaggtcgag cacacgcgcc gccgcatccg
     1141 tgagctcgac cgtcggcgcg gccgccgcct cgagaccgag cgcggcgaag acgtcgtccg
     1201 tccccaggag cggcgcggct ccctggcgga tcagccggtt cgttccgacc gagagggccg
     1261 tcgtgatctc gcccggcacg gcgaagacct cccgcccgag ctcgagcgcg aagtccgccg
     1321 tgatcagcgc gccggatcgc tcccgtgcct cgaccacaac ggtcgcgtgg gacagcccgg
     1381 cgatcacccg gttacgcgcc ggaaatcgcc acggtgcggg ctcaacgccc ggctcgtact
     1441 cggagacgac gaggcccgca ccggacacga tccgccgggc gagctccgcg tgccgcgccg
     1501 ggtagtcccg gtcgattccg cagccgagga cggcgagcgt cgtcccgccc gcctcgagcg
     1561 cgccgcggtg cgcctcggcg tccacgcccc gcgccatgcc gctcaccacg acgacgccgg
     1621 cggcggcgag ctcgcgggcg accgtccgcg cgacctgcgc gccgtacgcc gagcagctcc
     1681 gcgcgccgac gacggcgacg gcgggccgct cgaagatccc gtccggatca ccgcgcagat
     1741 gaagtcgtgc cggcgggtcg tggagctggg cgaggaggga cgggtaccct gcgccgccgc
     1801 gtcggagcac gccgtacgtc acgccgcgac cgcgagctcg ctcggcgagc ggtaggagag
     1861 cgcttccgcc atgtgctcgg gacagacctc gtcgacccct gcgagcgcac cgatcgtgac
     1921 ggcgacgccc cagatcctcg cgtgcccgcg cgcggagagc ccgagccggt cgacggcccg
     1981 ggcgagcacg gtgcccgccg cctcgccgat cggcggcggt cgctcgcgca ggcggcgatc
     2041 cgcgtcgatg acccgatctg cgaccgcgct cgagggctcg ccgggcgccc cggccacctc
     2101 ctgcgagcga gcgcgcggga ccgtgacgac gaggtcgaac cggtcgagga gcgcccggga
     2161 gagcttctgc cggtacgccg cgagtcgagc gggcgagcac gagcactcgg cgccgggatc
     2221 gccgcgcgcc ccgcacgggc aaaggttcat cgtcccgaca aggaggaacc gcgcgggaaa
     2281 cacgtggcgc ccgccgatcc gggcgatcgc caccatgccg tcctcgagcg gctggcgcag
     2341 cgcctcgagc gagggccgcg ggaactcgcc gagctcgtcc agcaggagca cgccccggtg
     2401 ggcgagcgtc gcctcgccgg gacgcgggcc gggcccgccg ccgacgaccg cgggcgtcga
     2461 ggcagtgtgg tgcggcgcgc gaaacggcgg gtgcgcgacg aggggctgtc cggccggaag
     2521 cgcccccgcg acggagtgga tccgcgtgac ctcgagcgcc tcctcgcgcg tgagcggcgg
     2581 gaggatcccc ggcaggcgtc gcgcgagcat cgtcttgccc gttcccggcg gcccgccgag
     2641 caggaggttg tggcgtccgg ccgcggcgat ctcgagcgcg cgccgcgccc gctcctgccc
     2701 gcgaacctcg cgcagatcgg gcggcggcgg gcccgcgtag gcgggcggct cgccgacggg
     2761 ctccggctcg tgacgtccgc ggaggtacgc gcccgcctcg gcgaggtgac ggacggggat
     2821 cggctcgatt ccggcgagcg ccgcctcggg cgccgactcg gcggcgcaga ggagcttcgc
     2881 gagcccgctt cgccgtgccg cctctgccgc gacg
//
LOCUS       DASTFC010000050         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_857971,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000050 DASTFC010000000
VERSION     DASTFC010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857971"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            454..1194
                     /locus_tag="VFO88_01425"
     CDS             454..1194
                     /locus_tag="VFO88_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET9544216.1"
                     /translation="MRTAVVLTIGNEIVSGDVENTNASWLARRLADLGVTVRLTAALG
                     DDIGEVAAFLGAELPKADLIFVTGGLGGTPDDLTREAVAAAFGVPCVEQEPLAGELRT
                     RFASRGLGEYAARWACLPEGAEPLANPLGGAPGFVLGPVHVLPGLPSEMEAMFDAIAD
                     RFRGAPVAAWRRRYRTTEGEIVAVLEEATARHPDVAVGSYPHFAGADGPTVEVVLKSR
                     DEAALAEVRAWLERELGNLRAVAPVEEA"
     gene            1191..1886
                     /locus_tag="VFO88_01430"
     CDS             1191..1886
                     /locus_tag="VFO88_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544217.1"
                     /translation="MTRIATLLAALALAGCGAAAEQPNDPAAGAGAPPPPPVSIPTTA
                     LIVCGPDGASVATPEVAASTDGVHVEIRNETQGRQIVHVQGAESGQGEGFSEGAHTRV
                     WTLPPGSATVSCADPADEAPDPTGASFEIIDVDGVWVSTALDCENVSTATFDWIAGAP
                     GVKGDPAEVVKAESEAQLEPGDVVEAAGYPEAEGFSTVRVVRDGRVISTVDLVPTDDG
                     GWLISTTSTCDEP"
     gene            1942..>2630
                     /locus_tag="VFO88_01435"
     CDS             1942..>2630
                     /locus_tag="VFO88_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9544218.1"
                     /translation="MAVASPEEYLAHLNPAQREAVTAPEGPVLIVAGAGSGKTRVLTH
                     RIVHEIRALGVKPNEILAITFTNRAANEMKRRLEALLGGVARTMWVMTFHAACGRILR
                     REAPRLGLKSNFTIYDSADQVRLVKSCLEELDKDPKRFAPRGIHAQISSAKNQLVSPD
                     EYLERVASFYDQTVAEVYKRYQERLHAANALDFDDLLVLTVEVLERFPEARARWQKVF
                     RAVLVDEYQDT"
BASE COUNT          377 a          976 c          928 g          349 t
ORIGIN      
        1 cgcgctcggc atggacgggg cggcggtctt ccggctcgac cagtcgccgg gcggcgtgag
       61 cgtcgtcgac tgggtcggcg agacaccggt cgtccgcgtc gtgaacggcc tcgcgcgcgc
      121 gtagcgggtc ggcctccgaa agggggtcag accccgcacc aaaccgccta tgaatgtgtg
      181 ccgacttcgt gacgtccctg ctcggagcgg gcttctcgac ccccgggaac ggtcacggaa
      241 atgtgacgcg ctcgccttca cggggtcaga ccccgtgacg gagcacgcgc gcctccgtgt
      301 cggaaccgaa acgtccctgc aaacgggcac gttcatgccc gtggaagtgt gtcggaagtc
      361 gcgcgtcgcc ggcccttacg gggtcagacc ccgtaaggag agcgccacga gcccctcccg
      421 gaacgagcgc gcccggctcc tatactcgcc cgcatgagga cggccgtcgt cctgacgatc
      481 gggaacgaga tcgtctccgg cgacgtcgag aacacgaacg cgtcctggct cgcccgccgc
      541 ctcgccgacc tcggggtcac cgttcgcctc accgccgcgc tcggcgacga catcggcgag
      601 gtcgccgcgt ttctcggcgc ggagctcccg aaggcggatc tgatcttcgt caccggaggc
      661 ctcggcggga cgccggacga cctgacgcgc gaggcggtcg ccgccgcgtt cggcgtcccc
      721 tgtgtcgagc aggagccgct cgccggcgag ctccgcaccc gcttcgcgtc gcgcgggctc
      781 ggcgagtacg ccgcgcgatg ggcctgcctc ccggagggcg ccgagcccct cgcgaacccg
      841 ctcggcggcg ctcccggctt cgtcctcggc ccggttcacg tcctgccggg cctgccgagc
      901 gagatggagg cgatgttcga cgcgatcgca gaccgcttcc gcggcgcgcc ggtcgccgcg
      961 tggcgtcgcc gctaccgcac gaccgagggg gagatcgtcg ccgtcctcga ggaggcgacc
     1021 gcgcgccacc cggacgtcgc cgtcgggagc tacccgcact tcgccggcgc ggacgggccg
     1081 accgtcgagg tcgtcctcaa gtcgcgggac gaggccgccc tcgccgaggt gcgcgcatgg
     1141 ctcgaacggg agctcgggaa cctccgcgcg gttgcgcccg tcgaagaggc gtgacccgca
     1201 tcgccaccct cctcgccgcg ctcgcgctcg ccgggtgcgg cgccgccgcc gagcagccca
     1261 acgatcccgc cgccggagcc ggggccccgc cgccgcctcc tgtgagcatc cccacgacgg
     1321 cgctgatcgt ctgcggcccg gacggggcgt cggtcgcgac gcccgaggtc gcggcgagca
     1381 cggacggtgt gcacgtcgag atccgcaacg agacgcaggg caggcagatc gtccacgtgc
     1441 agggagccga atctgggcag ggcgagggct tctccgaggg agcccatacg cgggtgtgga
     1501 cgctgccacc cggctcggcc acggtctcgt gcgccgaccc ggccgacgag gcgcccgacc
     1561 cgaccggcgc gtccttcgag atcatcgacg tggacggcgt atgggtctcg accgcgctcg
     1621 actgcgagaa cgtgtcgacg gcgacgttcg actggatcgc gggcgctccc ggcgtcaagg
     1681 gcgacccggc cgaggtcgtc aaagctgaga gcgaggcgca gctcgagccc ggcgacgtcg
     1741 tcgaggccgc cggctacccg gaggccgagg gcttctcgac cgtccgtgtc gtccgcgacg
     1801 gccgtgtgat ctcaacggtc gacctcgtgc cgaccgacga cggcggctgg ctcatctcga
     1861 cgacgtcgac ctgcgacgag ccgtagggcg cgtcccggcg ggacgagggg agcggccccg
     1921 cgagcgacta gcatcgagcc cgtggccgtc gcctcgccgg aggagtacct cgcgcacctg
     1981 aaccccgccc agcgggaggc cgtcaccgcg ccggagggcc ccgtcctgat cgtcgccggc
     2041 gctggctcgg ggaagacccg cgtcctcacg cacaggatcg tccacgagat ccgcgcgctc
     2101 ggcgtgaagc cgaacgagat cctcgcaatc acgttcacga accgggccgc gaacgagatg
     2161 aagcgccgcc tcgaggccct gctcggcggg gtcgcgcgga cgatgtgggt gatgacgttc
     2221 cacgccgcct gcggccggat cctccgccgc gaggcgccgc gcctcggcct caagtcgaac
     2281 ttcacgatct acgattcggc cgaccaggtc cggctcgtga agagctgcct cgaggagctc
     2341 gacaaggacc cgaagcgctt cgccccgcgc ggcatccacg cgcagatctc ctcggcgaag
     2401 aaccagctcg tctcgccgga cgagtacctc gagcgcgtcg cgtcgttcta cgaccagacg
     2461 gtcgccgagg tctataagcg ctaccaggag cggctccacg cagcgaacgc gctcgacttc
     2521 gacgacctcc tcgtcctcac ggtcgaggtg ctcgagcgct tccccgaggc gcgcgcccgc
     2581 tggcagaagg tcttccgcgc cgtgctcgtc gacgagtacc aggacacgaa
//
LOCUS       DASTFC010000051         6350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_742665,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000051 DASTFC010000000
VERSION     DASTFC010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742665"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFO88_01440"
     CDS             complement(<1..622)
                     /locus_tag="VFO88_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9544219.1"
                     /translation="MPSRLRTRLYWMKVRLLPYGVEGLWRRPVHRARWALRSPEALER
                     WVDRRLALGDDYWLFLLGVSNSGTTILTRLLASHPALRVLPKEGQALTDAVPSANRFG
                     LGRRWTERLDLFHLTEESDPAPALRAKYDWAYHFERRPGILVEKSPPNAVRTRWLQAN
                     FRPARFLAITRHPYAVCEGMRRRSKATIEDAARQWATANEVMLADAE"
     gene            complement(637..1839)
                     /locus_tag="VFO88_01445"
     CDS             complement(637..1839)
                     /locus_tag="VFO88_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9544220.1"
                     /translation="MIPPEVERRARWVLDTLGASDLGLGDDLPYVESAWEQAARGERP
                     EGDDLAEAFFHLARVEERDAERDRHGRFAAASSSLDPLDPPLERLRARLGLEPPRWGG
                     ARFAIALTHDVDVPWRWTRVGIRGGAARVKHAVLARRPRDVAAETRALAAVPLHKARG
                     TDPNWRFREICELERSLGTRSTFFLLAAHRHRADGPVPEAYESRRARLVETLRAGGAE
                     IGLHASYTAADDPAVLAEEQEALTALAGPLAGQRFHYLRVDPHRNLRELDRLGFRYDS
                     SLGFADAPGFRAGIAHPFRPWNFEREAPLGLVEVPLVAMDVTYAEERYLGLSARAAEP
                     RLLALVRQAAERGGGFAVLWHTDRFDRGTSRGWDRLYRRLIEAVGAAGGVCVSAGELA
                     AEAAERLA"
     gene            complement(1836..3065)
                     /locus_tag="VFO88_01450"
     CDS             complement(1836..3065)
                     /locus_tag="VFO88_01450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9544221.1"
                     /translation="MRILLVSLYWPPAGGPGVQRPLKLAGHLTALGFDVHVLAPDDPK
                     WVHRDASLRVPEGVTVHRARNLGPRARIPGHELYGRRGLDRLRRRATITARGLLVPDA
                     SVLWNATAIPAALRIAREERIDIVLTTSPPGSVHLVGAAVRTRGRARWVADLRDGIVG
                     HAHRRREIRGEPALARLVARRADAVVAASAGVAEGMERFAPGLTVHVVENGCDFDDFE
                     GLEYRRGERFRITHTGSFFGRRSPQAFLEALERTDGDVVARFVGGLPPRQRDWVESNG
                     LRDRVEVHDFLPRAEALALQRDSEVNLLLVPEAEGRGRAVLTAKVFEYLAAERPILAV
                     VPPDGHAAELVREAGAGVVVPPDDVGAIATALAELERRWREGTLDGSPLSPELRARLD
                     RRAGAERLAEILRGVAR"
     gene            complement(3087..3722)
                     /locus_tag="VFO88_01455"
     CDS             complement(3087..3722)
                     /locus_tag="VFO88_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9544222.1"
                     /translation="MTEGWSRVLVLAPHTDDGEFGCGGAIARMVDAGVDVRYVAFSVA
                     TKSLPEGFPPDTLAREVREATAAIGIPPDKLDVHDFEVRTFPVHRQEILELLIAIWEE
                     WRPDAVFMPSVRDIHQDHQVIAAEGLRAFKRTTVLGYEIPWNNLDFAYQAYISLEKAH
                     VERKVEALSRYASQQHRNYANPEHIWTRARTHGINIGRDYAEVFEVYRVVA"
     gene            complement(3736..3891)
                     /locus_tag="VFO88_01460"
     CDS             complement(3736..3891)
                     /locus_tag="VFO88_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544223.1"
                     /translation="MQGPDLLLAHMALLDEPADPPDERPTARERLDEELGPELAAELL
                     AALAPRR"
     gene            4010..4612
                     /locus_tag="VFO88_01465"
     CDS             4010..4612
                     /locus_tag="VFO88_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET9544224.1"
                     /translation="MAFAFSLSFGVLAKASGMGSLAPVLMSMTTFAGSAQFAAASVLG
                     TGGGALAAIGAASLLNTRYAPISLTVAPSMGGTWFSRLIQSQLIVDESWAVAQAGGGR
                     VDRRTLIGAGALLWVFWVGGTVVGVLGSEFIGNPARYGLDAAFPALFLALLVAQIDSR
                     KAVLAAVLGAAIALALVPFSAAGVPIIVASLACLVGLWER"
     gene            4609..4917
                     /locus_tag="VFO88_01470"
     CDS             4609..4917
                     /locus_tag="VFO88_01470"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET9544225.1"
                     /translation="MSEVWLVVLVVGAVTVALKAAGPVLLGGRELPPPVLRVVVLLAP
                     ALLAALVAVGLVGGDRELLLDERLAGLGAAVLALLVRAPILVVVIAAAVATALARGLA
                     "
     gene            complement(4889..5701)
                     /locus_tag="VFO88_01475"
     CDS             complement(4889..5701)
                     /locus_tag="VFO88_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET9544226.1"
                     /translation="MLELDFMRMAFAAGSVVGLLAPAVGFFVVQRRMSLIGDGIGHTA
                     FAGVAFGYLVGLSPVLTALVAAVGGAVAVEWLRARRGTAGDQALALLFYTGLAAGVVL
                     VSAAGRFDAGLFAFLFGAILTVTTSDLVLVGVLGAVGLATIGLLYRALAGIALDEEGA
                     HVAGVPVALLNVLLAALVGLTVAISMRIVGILLVAALMVLPVIAASRVARSMRASFLL
                     ALALGLASVWIGLTVSYYGNLASGGAIVLVAAAFVLAATAAGSVTRGRVPAR"
     gene            complement(5694..>6350)
                     /locus_tag="VFO88_01480"
     CDS             complement(5694..>6350)
                     /locus_tag="VFO88_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9544227.1"
                     /translation="RDACLTVEEGEFVAIAGPNGGGKTTLLRILLGLARPAAGTALLF
                     GEPAHRFSRRATLGYLAQRSRLGTDAPATVREVVSAGRLAAGGLLGPLRAGDKAIVAR
                     AIADVGLEEKADAPLATLSGGQQQRAFIAKALAAEPSLLVLDEPTAGVDPGAQESLAR
                     LLERLHRERQVTVLYVSHEFGAVENVVRRLVLVRGGIVFDGPPSDLPGAWHDPSHVHA
                     "
BASE COUNT          787 a         2353 c         2448 g          762 t
ORIGIN      
        1 tctccgcgtc ggcgagcatc acctcgttcg ccgtcgccca ctggcgcgcc gcgtcctcga
       61 tcgtcgcctt cgagcggcgg cgcatcccct cgcagacggc gtacgggtgc cgcgtgatcg
      121 cgaggaagcg cgccgggcgg aagttcgcct gcagccagcg cgtgcggacg gcgttcggcg
      181 ggctcttctc cacgaggatc cccggccgcc gctcgaagtg gtacgcccag tcgtacttcg
      241 cccggagcgc cggcgcggga tcgctctcct ccgtcaggtg gaagaggtcg agccgctccg
      301 tccagcgccg gccgaggccg aaccggttcg cgctcgggac ggcgtcggtg agcgcctggc
      361 cctccttcgg caggacgcgg agcgccgggt gcgacgcgag cagccgggtc aggatcgtcg
      421 tgccggagtt cgagacgccg agcaggaaga gccagtagtc gtccccgagg gcgagccggc
      481 ggtcgaccca gcgctcgagc gcctcggggc ttcgaagcgc ccagcgcgcg cggtgcaccg
      541 gtcgccgcca gaggccctcg acgccgtacg ggaggagccg gaccttcatc cagtagaggc
      601 gcgtgcggag gcgcgacggc atgccggcga tcctagtcag gcgaggcgct cggcggcctc
      661 ggctgcgagc tcgccggccg agacgcagac gccgccggcg gccccgacgg cctcgatgag
      721 ccgccgatag aggcggtccc agccgcgcga cgtgccgcgg tcgaagcggt cggtgtgcca
      781 gaggacggcg aagccgccgc cgcgctcggc cgcctgccgc acgagcgcga ggaggcgcgg
      841 ctcggctgcg cgcgcggaga gcccgagata gcgctcctcc gcgtacgtca cgtccatcgc
      901 cacgagcggg acttcgacga gcccgagcgg cgcctcgcgc tcgaagttcc agggacggaa
      961 cgggtgcgcg atcccggcgc ggaacccggg cgcgtccgcg aagccgaggg agctgtcgta
     1021 gcggaacccg agccggtcga gctcgcggag gttccggtgc gggtcgacgc ggaggtagtg
     1081 gaaccgctgc ccggcgagcg gccccgcgag cgccgtgagc gcctcctgct cctcggcgag
     1141 gacggccggg tcgtcggcgg ccgtgtagct cgcgtggagc ccaatctccg cgccgcccgc
     1201 ccgcagcgtc tcgacgaggc gggcgcggcg gctctcgtac gcctccggga cgggcccgtc
     1261 ggcgcggtgc cggtgggcgg cgaggaggaa gaacgtcgag cgcgtgccga gcgagcgctc
     1321 gagctcgcag atctcgcgga agcgccagtt cgggtcggtg ccgcgcgcct tgtgcagcgg
     1381 gacggccgcg agcgcgcgcg tctcggcggc cacgtcgcgc ggccggcggg cgaggacggc
     1441 gtgcttcacg cgcgccgcgc cgccgcggat cccgacacgc gtccagcgcc agggaacgtc
     1501 gacgtcgtgc gtgagcgcga tcgcgaagcg ggcgccgccc cagcgcggcg gctcgagccc
     1561 gagcctcgcc cgcagccgct cgagcggcgg gtcgaggggg tcgagcgacg aggacgcggc
     1621 ggcgaagcgg ccgtgccggt cgcgctcggc gtcgcgctcc tcgacgcggg cgaggtgaaa
     1681 gaacgcctcg gcgaggtcgt ccccctccgg gcgctcgccg cgcgcggcct gctcccaggc
     1741 gctctcgacg tacgggaggt cgtccccgag cccgaggtcg ctcgcgccga gcgtgtcgag
     1801 cacccagcgg gcgcgccgct cgacctcggg cgggatcacc gggcgacccc gcggaggatc
     1861 tccgcgagcc gctccgcgcc cgcgcgccgg tcgagccggg cgcggagctc gggcgagagc
     1921 ggcgagccgt cgagcgtccc ctcccgccag cgccgctcga gctcggcgag cgcggtcgcg
     1981 atcgcgccga cgtcgtcggg cggcacgacg acgcccgctc cggcctcccg gacgagctcg
     2041 gccgcgtgcc cgtcgggcgg gacgaccgcg aggatcggcc gctcggccgc gaggtactcg
     2101 aagaccttcg ccgtcagcac cgcgcgcccg cggccctcgg cctccgggac gagcaggagg
     2161 ttcacctcgg agtcgcgctg gagcgcgagc gcctccgcgc gcgggaggaa gtcgtggacc
     2221 tcgacgcggt cgcgcagccc gttcgactcg acccagtcgc gctggcgggg cgggaggccg
     2281 ccgacgaagc gcgcgacgac gtcgccgtcc gtccgctcga gcgcctcgag gaacgcctgc
     2341 gggctccgtc gcccgaagaa gctccccgtg tgcgtgatcc ggaaccgctc gccgcgccgg
     2401 tactcgagcc cctcgaagtc gtcgaagtcg cagccgttct cgaccacgtg gacggtgagc
     2461 cccggcgcga agcgctccat cccctcggcc acgccggccg acgcggcgac gaccgcgtcc
     2521 gcgcgccgcg cgacgagccg cgcgagcgcc ggctcgccgc ggatctcccg ccgccggtgc
     2581 gcatggccga cgatcccgtc acggaggtcg gcgacccagc gcgcccggcc gcgcgtgcga
     2641 acggccgcgc ctacgaggtg caccgagccg ggcggcgagg tggtcaggac gatgtcgatc
     2701 cgctcctcgc gcgcgatccg cagcgccgcc gggatcgcgg tggcgttcca gagcacgctc
     2761 gcgtccggca cgagcagccc gcgcgccgtg atcgtcgcgc gacgccgcag ccggtcgagc
     2821 ccgcggcggc cgtagagctc gtgcccgggg atccgcgcgc gcggcccgag gttccgggcg
     2881 cggtggacgg tcacgccctc cggcacgcgg agcgaggcgt cgcggtgcac ccacttcggg
     2941 tcgtcaggcg cgagcacgtg cacgtcgaag ccgagcgccg tcaggtgccc ggcgagcttc
     3001 agcggccgct ggacgcccgg cccgcccgcg ggcggccagt agagcgagac gaggaggatc
     3061 cgcacgccgc cattctcgcg tggcggctag gcgacgacgc ggtagacctc gaacacctcg
     3121 gcgtagtcgc gcccgatgtt gatgccgtgc gtgcgggcgc gcgtccagat gtgctcgggg
     3181 ttcgcgtagt tgcggtgctg ctgcgaggcg tagcgggaga gggcctcgac cttccgctcg
     3241 acgtgcgcct tctcgagcga gatgtacgcc tggtaggcga agtcgaggtt gttccagggg
     3301 atctcgtagc cgagcacggt cgtccgcttg aacgcccgca ggccctcggc ggcgatcacc
     3361 tggtggtcct ggtggatgtc gcgcacgctc ggcatgaaca ccgcgtccgg gcgccactcc
     3421 tcccagatcg cgatcaggag ctcgaggatc tcctgccggt gcacggggaa cgtccgcacc
     3481 tcgaagtcgt ggacgtcgag cttgtccggc gggatgccga tcgccgcggt cgcctcgcgc
     3541 acctcgcggg cgagcgtgtc cggcgggaag ccctccggca gcgacttcgt ggcgacggag
     3601 aacgcgacgt agcggacgtc gacgcccgcg tcgaccatcc gcgcgatcgc tccgccgcac
     3661 ccgaactcgc cgtcgtcggt gtgcggggcg agcacgagca cgcggctcca gccctcggtc
     3721 acgtgcgggc cccggtcatc ggcgcggcgc gagggcggcg agaagctcgg cggcgagctc
     3781 cggcccgagc tcctcgtcca gtcgctcgcg cgccgtcggc cgctcgtccg gcgggtcggc
     3841 gggctcgtcg aggagcgcca tgtgcgccag gaggagatcg ggaccctgca tcgttagggg
     3901 aagtatcgcc gcctcgcccg acatcgtctc gccgcgaccg tccgcggcgg cccgcgggga
     3961 gccgacgcgc taccgcgacg gcttccgcgc gatcgcgccc atcgtcccga tggcgttcgc
     4021 cttcagcctc tcgttcggcg tgctcgcgaa ggcgtcgggg atgggctcgc tcgcgcccgt
     4081 cctcatgtcc atgacgacgt tcgccggctc ggcccagttc gccgcggcgt ccgtgctcgg
     4141 gacgggaggc ggcgcgctcg cggcgatcgg cgccgcctcg ctcctgaaca cgcgctacgc
     4201 gccgatctcg ctcacggtcg cgccgtccat gggcggcacg tggttctcgc gcctcatcca
     4261 gtcgcagctc atcgtggacg agtcctgggc ggtcgcccag gcgggcggcg gacgcgtcga
     4321 ccggcggacg ctcatcggcg ccggggccct cctctgggtc ttctgggtcg gcgggacggt
     4381 cgtcggcgtc ctcggctcgg agttcatcgg caacccggcg cgctacgggc tcgacgccgc
     4441 gttcccggcg ctcttcctcg cgctcctcgt cgcgcagatc gacagccgga aggccgtgct
     4501 cgccgcggtg ctcggcgcgg cgatcgccct cgcgctcgtg ccgttctcgg cggccggcgt
     4561 gccgatcatc gtcgcgtcgc tcgcctgtct cgtggggctc tgggaacgat gagcgaggtc
     4621 tggctcgtcg tcctcgtcgt cggggcggtc accgtcgcgc tcaaggcggc cggcccggtg
     4681 cttctcggcg gtcgcgagct gccgccgccc gtcctgcgcg tcgtcgtcct gctcgcgccg
     4741 gcgctcctcg cggcgctcgt cgcggtcggg ctcgtcggcg gcgaccggga gctcctcctc
     4801 gacgagcggc tggcggggct cggcgcggcc gtgctcgcgc tgctcgtccg cgcgccgatc
     4861 ctcgtcgtcg tgatcgcggc ggcggtcgct accgcgctgg cacgcggcct cgcgtgaccg
     4921 agccggcggc ggtcgcggcg agcacgaacg ccgcggcgac gaggacgatc gccccgccgg
     4981 acgcgaggtt cccgtagtac gagaccgtga ggccgatcca gaccgacgcg agcccgagcg
     5041 cgagcgcgag gaggaacgac gcgcgcatgc tccgcgcgac gcggctcgcg gcgatcacgg
     5101 ggagcaccat gagcgcggcg acgaggagga tgcccacgat ccgcatcgag atggcgacgg
     5161 tgaggccgac gagcgcggcg aggagcacgt tcaggagcgc gaccgggacg cccgcgacgt
     5221 gcgcgccctc ctcgtcgagc gcgatgcccg cgagggctcg gtagaggagg ccgatcgtcg
     5281 cgagcccgac cgcgcccagg acgccgacca gcacgaggtc gctcgtcgtc accgtgagga
     5341 tggcgccgaa gaggaaggcg aagagccccg cgtcgaagcg cccggccgcg gagacgagca
     5401 cgacgcccgc ggcgaggccg gtgtagaaga ggagcgcgag cgcctggtcg ccggccgtcc
     5461 cgcggcgcgc gcgcagccac tcgaccgcga cggcgccgcc gaccgccgcg acgagcgccg
     5521 tcaagacggg cgagaggccg acgaggtagc cgaacgcgac gccggcgaac gcggtgtggc
     5581 cgatcccgtc tccgatgagg ctcatgcggc gctggacgac gaagaacccg accgccggcg
     5641 cgaggagccc gacgaccgag ccggccgcga aggccatccg catgaagtcg agctcaagca
     5701 tggacgtgcg acgggtcgtg ccaggcgccg ggcaggtcgg agggcggccc gtcgaagacg
     5761 atcccgccgc ggacgagcac gagccggcgc acgacgttct cgaccgcgcc gaactcgtgc
     5821 gagacgtaga ggacggtcac ctgccgctcg cggtgcagcc gctcgaggag ccgcgcgagc
     5881 gactcctgcg cgcccgggtc gacgcccgcg gtcggctcgt cgaggacgag gagcgagggc
     5941 tcggcggcga gcgccttcgc gatgaaggcc cgctgctgct ggccgcccga gagcgtcgcg
     6001 aggggcgcgt ccgccttctc ctcgagcccg acgtcggcga tcgcccgggc aacgatcgcc
     6061 ttgtccccgg cacggagcgg gcccaggagc ccgccggcgg cgagacggcc cgcgctcacg
     6121 acctcgcgga cggtggcggg cgcgtccgtc ccgaggcggc tgcgctgcgc gaggtacccg
     6181 agcgtcgcgc ggcgcgagaa gcggtgcgcc ggctccccga agaggagcgc cgtgcccgcg
     6241 gccgggcggg ccaggccgag aaggatgcgc aggagcgtcg tcttcccccc gccgttcggc
     6301 ccggcgatcg cgacgaactc gccctcctcg accgtgaggc acgcgtcgcg
//
LOCUS       DASTFC010000052         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1220648,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000052 DASTFC010000000
VERSION     DASTFC010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220648"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFO88_01485"
     CDS             <1..912
                     /locus_tag="VFO88_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544228.1"
                     /translation="VAEAVRSVEGAAALAPELAEVAAELGEAEIRVREGASSLRAFLA
                     SLEAEPGRLEAVEGELERVADARRRFGAATTDELLARAAAARAELEQVEAGVDPAQAA
                     EQAVAEAEARVGALSAELGAARADAAPAFAESVAGELQGLGMGDGEFEVELSEREPGP
                     SGTDGVAFLVRPNPGLPFAPAAETASGGELSRIALALRAVAHAAAGEPTIVFDEIDAG
                     IGGETAHAVADALRTLAGRAQIVTITHLPQIASVADAHFRVEKVPGDPTHTRIELLSD
                     EERRRELERMLGGADFLAGVAQRADEG"
     gene            912..1355
                     /locus_tag="VFO88_01490"
     CDS             912..1355
                     /locus_tag="VFO88_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9544229.1"
                     /translation="MEARATRELPSARSDVWALVAEPYHLTDWWPGYRGVRPDRRGLA
                     ADARWTVRRAPAAGFLAQASWRRPDAEGLIVITLVDPGLELRWHDVQQNTDVALALAN
                     AGEGRTRATVTMNGPWWRMLPEGGRDLPARSLQRLHDLCQTAADL"
     gene            1454..3100
                     /locus_tag="VFO88_01495"
     CDS             1454..3100
                     /locus_tag="VFO88_01495"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9544230.1"
                     /translation="MSARPTKYVFVTGGVVSSLGKGITAAALGRLLKARGLRVQVQKF
                     DPYLNVDPGTMSPFQHGEVFVTEDGAETDLDIGHYERFVDENLSRNANHTSGSIWYSV
                     IRKERKGEYLGSTVQVIPHVTNEIKDRIRRVGASSDADVVISEIGGTVGDIESLPFLE
                     AIRQFRREAGAENVMYVHVTYVPWIETAGELKTKPTQHSVNELRRIGIHPDVVVCRSN
                     EPLSYDIREKIALFADVDSRAVIVNEDVSDVYLVPVRLQEEGFDALVCERLGLDGEAP
                     DLGEWNELVSRIGKREGEIEIALVGKYVKLADAYLSVHEALKHSGIHHGVNVRVRWVD
                     AEGMSVEEAAAELERSHGVLVPGGFGARGWEGKIVACQVARERGIPYLGICLGMHVAV
                     SEFARNVVGLDGANSTEMDPETPYPVIDLLPEQKEVEDLGGTMRLGAQAVDLAEGSRV
                     RDAYGEAVIYERHRHRYEVNNHFRPQLVEAGLVVSGTYQEGRLVEIIELPGHPWFVAS
                     QFHPEFKSRPTRPAPLFREFVGAAAERARERVGGLHTTAG"
     gene            3271..>3592
                     /locus_tag="VFO88_01500"
     CDS             3271..>3592
                     /locus_tag="VFO88_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544231.1"
                     /translation="MSHALKLFLELAAVPSPPGSERAVADRVLAELRDLGLDATEDDA
                     GDRIAGDTGNILARLPASDPAAPDGGTPIFLCAHLDTVPPTDDIEPVVEDGVVRNARD
                     TILGA"
BASE COUNT          493 a         1298 c         1335 g          466 t
ORIGIN      
        1 gtcgcggagg ccgtgcggtc cgtcgagggc gcggccgcgc tcgcccccga gctggcggag
       61 gtcgccgccg agctcggcga ggcggagatc cgcgtccgcg agggcgcctc gtcgctccgc
      121 gccttcctcg cctcgctgga ggccgagccc ggcaggctcg aggcggtcga gggcgagctc
      181 gagcgggtcg ccgacgcgcg gcggcgcttc ggcgcggcga cgactgacga gctgctcgcg
      241 cgtgcggcgg ccgcacgcgc cgagctcgag caggtggagg ccggcgtcga cccggcgcag
      301 gccgcggagc aggcggtcgc ggaagccgag gcgcgcgtgg gcgcgctctc ggccgagctc
      361 ggcgccgctc gcgccgacgc ggcgcccgcg ttcgcggagt ccgtcgccgg tgagcttcag
      421 ggcctcggca tgggtgacgg cgagttcgag gtggagctct cggagcgcga gccggggccg
      481 agcgggacgg acggcgtcgc cttcctcgtc cgcccgaatc cgggcctccc gttcgcgccg
      541 gcggcggaga ccgcctcggg cggcgagctc tcgcgcatcg cgctcgcgct gcgcgccgtc
      601 gcgcacgccg ccgccggcga gcccacgatc gtcttcgacg agatcgacgc gggcatcggc
      661 ggcgagacgg cacacgcggt cgcggacgcg cttcgcacgc tcgcgggccg ggcgcagatc
      721 gtcacgatca cgcacctccc gcagatcgcg agcgtcgccg acgcgcactt ccgggtcgag
      781 aaggtgcccg gtgacccgac gcacacgcgg atcgagctgc tctccgacga ggagcggcgt
      841 cgcgagctcg agcggatgct cggcggcgcc gacttcctcg ccggcgtcgc gcagcgggcc
      901 gacgagggct gatggaggcg cgcgccacgc gggagctccc gtctgcgcgt tccgacgtct
      961 gggcgctcgt ggcggagccg taccacctca cggactggtg gcccggctac cgcggcgtcc
     1021 ggccggaccg gcgcggcctg gcggcggacg cgcgctggac cgtgcgacgc gccccggcgg
     1081 cgggattcct cgcgcaggcg tcgtggcggc ggcccgacgc ggaggggctc atcgtgatca
     1141 ccctcgtcga cccggggctc gagctccgct ggcacgacgt ccagcagaac accgacgtcg
     1201 cgctcgcgct cgcgaacgcg ggggagggcc gcacgcgcgc gacggtgacg atgaacggcc
     1261 cctggtggcg gatgctcccg gagggcggcc gcgaccttcc cgcccgcagc ctgcagcgcc
     1321 tccacgacct ctgccagacc gcggcggacc tctagcgccg actgacttgc gccgccccgc
     1381 ccgccggctc gtccccggcg gctggtacgc tccgatcccg tgggtcggtc acgggaatcg
     1441 ggggtctgag gtcgtgagcg cgcggccgac gaagtacgtc ttcgtcaccg gcggcgtcgt
     1501 ctcgtccctc ggcaagggca tcaccgccgc ggcgctcggc cggctgctca aggcgcgcgg
     1561 gctgcgtgtc caggtgcaga agttcgaccc gtacctcaac gtcgatccgg gcacgatgag
     1621 cccgttccag cacggcgagg tcttcgtcac cgaggacggc gccgagaccg acctcgacat
     1681 cggccactac gagcgcttcg tcgacgagaa cctctcgcgc aacgcgaacc acacctcggg
     1741 ctcgatctgg tactcggtca tccgcaagga gcgcaagggc gagtacctcg ggtcgaccgt
     1801 gcaggtgatc ccgcacgtca cgaacgagat caaggaccgc atccggcgcg tcggggcgtc
     1861 gagcgacgcg gacgtcgtca tctccgagat cggggggacg gtcggcgaca tcgagtcgct
     1921 cccgttcctc gaggcgatcc gccagttccg ccgcgaggcg ggggcggaga acgtcatgta
     1981 cgtccacgtc acgtacgtcc cgtggatcga gacggccggc gagctcaaga cgaagccgac
     2041 gcagcactcc gtgaacgagc tcaggcgcat cggtatccac ccggacgtcg tcgtctgccg
     2101 ctcgaacgag ccgctctcgt acgacatccg ggagaagatc gcgctcttcg ccgacgtcga
     2161 ctcgcgcgcc gtgatcgtga acgaggacgt gagcgacgtg tacctcgtcc cggtgaggct
     2221 gcaggaggag ggcttcgacg cgctcgtctg cgagcggctc gggctcgacg gcgaggcgcc
     2281 cgacctcggc gagtggaacg agctcgtgtc gcggatcggc aagcgcgagg gggagatcga
     2341 gatcgcgctc gtcggcaagt acgtgaagct cgcggacgcg tacctctccg tacacgaggc
     2401 gctcaagcac tcggggatcc accacggggt gaacgtccgc gtccgctggg tcgacgccga
     2461 ggggatgagc gtcgaggagg cggccgcgga gctcgagcgc tcgcacggcg tgctcgtccc
     2521 cggcgggttc ggcgcgcgcg gctgggaggg gaagatcgtc gcctgccagg tggcgcgcga
     2581 gcgcgggatc ccgtatctcg gcatctgcct cgggatgcac gtcgccgtct cggagttcgc
     2641 gcggaacgtc gtcgggctcg acggcgcgaa ctcgacggag atggatccgg agacgccgta
     2701 tccggtcatc gacctcctgc cggagcagaa ggaggtcgag gatctcggcg gaacgatgcg
     2761 cctcggcgcg caggcggtcg acctcgcgga gggctcgaga gtgcgcgacg cgtacggcga
     2821 ggccgtgatc tacgagcgcc accggcaccg ctacgaggtg aacaaccact tccgtccgca
     2881 gctcgtcgag gcaggcctcg tcgtctccgg gacgtaccag gaggggcgcc tcgtcgagat
     2941 catcgagctg cccggccacc cgtggttcgt ggcgagccag ttccacccag agttcaagtc
     3001 gcggcccacg cggcccgcgc cgctcttccg cgagttcgtc ggcgccgccg ccgagcgcgc
     3061 ccgcgagcgg gtcggcggcc tccacacgac cgccgggtag ccgggccctc ccgcacccgg
     3121 gctagacgca cgcgggtaac gggaggttcg cccccacccc tgagattcat cgtatcggcg
     3181 gaaagcgcac gtgtctgtgc caaaccggtg ccggagtcgt gccgactcac ggcgggtcga
     3241 ccgtcgagtc gtgcggccta gaatcgccgg gtgtcccacg ccctcaaact cttcctcgag
     3301 ctggcggccg tcccgagccc gccggggagc gagcgggccg tcgccgatcg cgtcctcgcc
     3361 gagctccgcg acctcggact ggacgccacg gaggacgacg ccggcgaccg gatcgcgggc
     3421 gacaccggga acatcctcgc gcggctcccg gcgtccgacc cggcggcccc cgacggcggg
     3481 acgccgatct tcctctgcgc tcacctcgac accgttccgc cgacggacga catcgagccc
     3541 gtcgtcgagg acggtgtcgt ccgcaacgcg cgcgacacga tcctcggcgc gg
//
LOCUS       DASTFC010000053         6690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_248234,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000053 DASTFC010000000
VERSION     DASTFC010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248234"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="cofH"
                     /locus_tag="VFO88_01505"
     CDS             <1..906
                     /gene="cofH"
                     /locus_tag="VFO88_01505"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HET9544232.1"
                     /translation="GAVEICLQGGIHPAFTGETYLGIARAVKDAVPGLHVHAFSALEV
                     WQGAATLGEPLDAYLERLRDAGLASLPGTAAEILDDEVRATLCPDKISTAQWLAVHDA
                     AHRAGLRSTTTMMFGSVEGPRSWARHLLALRDQQRATGGFTEFVPLPFVHMEAPIFLK
                     GLARPGPTFREAMLVHAAGRLVLHPWITNVQASWVKLGVAGAQAALRAGANDLGGTLM
                     NESISRAAGAAHGEEFPPEAMEAAIRAVGRAPRQRTTLYGTPEPDRTTASFGAPALAP
                     PWNPSVNDARLRRPRRLVRPGVTAV"
     gene            complement(911..1309)
                     /locus_tag="VFO88_01510"
     CDS             complement(911..1309)
                     /locus_tag="VFO88_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET9544233.1"
                     /translation="MRRRVAAARVARLASVDPAGRLHVVPICFALDGETLVSAVDAKP
                     KGSTRPQRLVNVRASPEVALLVDEYDEDWSRLWWVRLRGRARVVEEEPEREDALALLA
                     AKYPQYRDEPPPGPAIVVELTEWRGWAAVP"
     gene            complement(1335..2399)
                     /locus_tag="VFO88_01515"
     CDS             complement(1335..2399)
                     /locus_tag="VFO88_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9544234.1"
                     /translation="MNGNGLRFSYVMLPDYPLVDSIEMIKTADELGFYGCYAADETWH
                     KDLWLLFAAAADKTTNIRFGPSLSPIGLREPTLIVQALATLDELTNGRAECVFSIGNF
                     GLLAQYGIDWAKVKPLSRVREAHQVMRTFLDQGTIDFDGEFYNYSGLFTFARPVQEHV
                     PLLVGAMRGPKSFQFAGEASDGVHHALSYSREAYDYLVENVRVGAEKAGRDWKELDIG
                     AWCVCVVSPDGDAAKEAARSMVGFYISSMPREQLARHGLDADDLQPIVEALGRGAIDE
                     AIELTSADVAARLSLAGTPEEVAAKIRDDIAGAGVNHMILAITDPAMVKAFTTRDLEG
                     VPDVRGQLKLIHDEVMPAFA"
     gene            complement(2440..2724)
                     /locus_tag="VFO88_01520"
     CDS             complement(2440..2724)
                     /locus_tag="VFO88_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544235.1"
                     /translation="MIGFTLRAQRPPEARRVNENVVERFEHVFEVAPEKMTEHVQQQD
                     IPAWDTQRIVGSRWDHLDWMHDHWADSVLSTGIEPEIAELGETMPSEERS"
     gene            complement(2721..4256)
                     /locus_tag="VFO88_01525"
     CDS             complement(2721..4256)
                     /locus_tag="VFO88_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9544236.1"
                     /translation="MTATATALGDGVLDELRGILGGDEYVLPDLPARVNRTRVPAPFP
                     VHRWGEFLPDVAVLPRTTEQVSEVVKLANRHGIPIVPRAGGTGLADGAVPLRGGIVVD
                     VKLMNRILDIDLVDRTVTVQTGINMLTLNRELKAHGVIYPDDPASYPCSIVGGRIGTS
                     GWSLIGGRYGHTRDLVISMELVLPTGEVIEVGDGGGRKVRKSSTGFQLKHLFMGHQGT
                     LGIATEATLELVPRPEVEFAAFFAFSDYRTAWEATGALAKSGLATLAGVVLFDEWKLA
                     YLRRDDEAYIPQPPTVRAVIASAMYGARDEVEPASRRLMRIAKEAGGKYLGDEISQGD
                     WASRHDRYATPLHGRLKDGQVVPMCWHCEDASIPYSQLPAVREKWHAIAERYIDRYGI
                     FDDWGMFAYTNAAYKPWGDYLTEIDIGIWEQELDEENWRGWVECKREIAEVALEHGGS
                     ITACHGATRAGDVELVPQEMGGGFEVMKKIKAALDPNNVMNPGKYLLDQAYEGENGRE
                     AVR"
     gene            complement(4253..5539)
                     /locus_tag="VFO88_01530"
     CDS             complement(4253..5539)
                     /locus_tag="VFO88_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9544237.1"
                     /translation="MEATQGGAVAENEVRETGTGQGANGRDGREAAFAAEWRNAAYNC
                     FSSGHKFCREVCPVMQVTRNENHSPTAFHANVVAMEQGLVSVEDTARDYVHCTQCGAC
                     ELRCPNTLFTGDFYRYRTRTVDVVKAMRALAVEQGVEQPAWKRWNELTNERRNEPVLG
                     DVPVSQEHVADWAAGLDLPVGGETVLFCDCEAAFHRTSLPRAVARILQAGGVEFGLMR
                     EQWCCGGPAAEMGYVDQARRFAEHNVADWRAVGARRILVLDPHDYIAFTEDYPRFFGD
                     EFDFEIVLVVELVAELVREGKLALTRPVERTVTYHDACRLNKRKGIHEAPREILRAIP
                     GITFKDVDHVSQWSYCSGAGGGLPIERPDLTAEISRRRVERAAELDVDTLVSACVWSE
                     RPLSEQGAAGERPLEVMDLMELVAVSAGLDDREEAA"
     gene            5726..>6690
                     /locus_tag="VFO88_01535"
     CDS             5726..>6690
                     /locus_tag="VFO88_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544238.1"
                     /translation="MLGGNSGRRLTLGVLVLAISLVFGSSLAGARTVEPDDGLQDRDS
                     MGSSMDPSGALSALDAESDVVSSGPSAKVTKNLAVAGRGERLMPNATTDVWAHDGYAY
                     LGTFNEPCGDGTGANGSGVHIFDVSNPNKVTPAGQLPSVLGSRINDIKVADMNSGTIL
                     VHSNESCGGPEGGFEVYDMSNPLSPVHLASARVDEANATLRDLFGGTNLGVHNLYLFT
                     QGDRDYVAMQTHAWFGSFQIWELTDPSNPQFVSAWGAELLCEEAYCSDDPYNETDVTT
                     LVNHINGYMFGLLTPAYGFSQNRFLHDMTVTADGTKAYLAHWDAGL"
BASE COUNT          931 a         2481 c         2291 g          987 t
ORIGIN      
        1 ggcgccgtcg agatctgcct ccagggcggg atccaccccg ccttcaccgg cgagacgtac
       61 ctcgggatcg cccgcgcggt gaaggacgcc gtgccgggcc tccacgttca cgcgttctcc
      121 gcgctcgagg tctggcaggg cgcggcgacg ctcggcgagc cgctcgacgc ctacctcgag
      181 cggctgcgcg acgcggggct cgcctcgctc ccgggcacgg cggccgagat cctcgacgac
      241 gaggtccgcg cgacgctctg cccggacaag atctcgaccg cgcagtggct cgccgtccac
      301 gacgccgcgc accgggccgg cctgcgctcg acgacgacga tgatgttcgg ctcggtcgag
      361 ggcccgcgct cgtgggcgcg ccacctgctc gccctgcgcg accagcagcg ggcaaccggc
      421 ggcttcacgg agttcgtgcc gctgccgttc gtgcacatgg aggcgcccat cttcctcaag
      481 ggcctcgccc gccccggccc gacgttccgc gaggcgatgc tcgtccacgc ggccggacgg
      541 ctcgtcctcc acccgtggat cacgaacgtc caggcgtcct gggtcaagct cggcgtcgcc
      601 ggcgcgcagg ccgcgctccg cgccggcgcg aacgacctgg gcgggacgct catgaacgag
      661 tcgatctcgc gcgcggccgg cgcggcccac ggcgaggagt ttccgccgga ggcgatggag
      721 gccgcgatcc gcgccgtcgg gcgcgcgccc cgccagcgga cgacgctcta cggcacgccc
      781 gagcccgacc gtacgaccgc gagcttcggc gcgccggcgc tcgcgccgcc ctggaacccc
      841 agcgtgaacg acgcccgcct ccggcgcccc cgccggctcg tccggccggg cgtcacggcc
      901 gtctgaggcg ctacgggacc gcggcccagc cgcgccactc cgtcagctcc acgacgatcg
      961 ccggccccgg cggaggctcg tcgcggtact gcgggtactt cgccgcgagg agcgcgagcg
     1021 catcctcacg ctccggctcc tcctcgacca cgcgcgcccg cccgcgcagc cgcacccacc
     1081 agaggcggct ccagtcctcg tcgtactcgt cgacgaggag cgcgacctcc gggctcgcgc
     1141 ggacgttgac gagccgctgc gggcgagtcg atcccttcgg cttcgcatcg accgccgaaa
     1201 cgagcgtctc gccgtcgagg gcgaagcaga tcggcacgac gtgcagccgg cccgccgggt
     1261 cgacggacgc gagccgggcg acgcgcgccg ccgcgacgcg gcgcctcatc tcggggccgg
     1321 agagcgcgtt ggcgctacgc gaacgcaggc atcacctcgt cgtggatgag cttcagctgg
     1381 ccgcgcacgt ccggcacgcc ttccaggtcg cgcgtcgtga aggccttcac catcgccggg
     1441 tcggtgatcg cgaggatcat gtggttcacg ccggcgcccg cgatgtcgtc gcggatcttc
     1501 gccgcgacct cctcgggcgt cccggcgagg gagagccggg cggccacgtc cgctgacgtc
     1561 agctcgatcg cctcgtcgat cgccccgcgg ccgagcgcct cgacgatcgg ctgcaaatcg
     1621 tccgcgtcga ggccgtggcg ggccagctgc tcgcgcggca tcgaggagat gtagaagccg
     1681 accatgctgc gcgccgcctc cttcgccgcg tcgccgtcgg gcgagacgac gcagacgcac
     1741 cacgcgccga tgtcgagctc cttccagtcg cggcccgcct tctccgcccc cacgcggacg
     1801 ttctcgacga ggtagtcgta cgcctcgcgc gagtagctga gcgcgtggtg caccccgtcc
     1861 gacgcctcgc cggcgaactg gaacgacttc gggccgcgca tggcgccgac gaggagcggg
     1921 acgtgctcct gcaccggccg cgcgaacgtg aagaggccgc tgtagttgta gaactcgccg
     1981 tcgaagtcga tggtgccctg gtcgaggaac gtccgcatga cctggtgcgc ctccctcacc
     2041 cgcgacagcg gcttcacctt cgcccagtcg atcccgtact gcgcgagcag cccgaagttc
     2101 ccgatcgaga agacgcactc ggcgcggccg ttcgtcagct cgtcgagcgt cgcgagcgcc
     2161 tggacgatga gcgtcggctc ccgcaggccg atcggcgaga ggctcggacc gaagcggatg
     2221 ttcgtcgtct tgtccgccgc cgccgcgaac aggagccaga gatccttgtg ccaggtctcg
     2281 tccgccgcat agcacccgta gaacccgagc tcgtccgcgg tcttgatcat ctcgatcgag
     2341 tccacgagcg ggtagtcggg gagcatcacg tagctgaacc tcaatccgtt gccgttcacg
     2401 ccgcctcctt cattcgtctc cgtccttccg ggcccccgcc tagctgcgct cctcgctcgg
     2461 catcgtctcg ccgagctccg cgatctccgg ctcgatcccc gtcgagagga cgctgtcggc
     2521 ccagtggtcg tgcatccagt cgaggtggtc ccaccggctc ccgacgatcc tctgcgtgtc
     2581 ccaggccggg atgtcctgct gctggacgtg ctcggtcatc ttctcgggcg cgacctcgaa
     2641 cacgtgctcg aaccgctcga cgacgttctc gttcacgcgc cgcgcctcgg gcgggcgctg
     2701 cgcgcgcagc gtgaacccga tcatcggacc gcctcccgtc cgttctcgcc ctcgtacgcc
     2761 tgatccagga ggtacttgcc ggggttcatg acgttgttcg ggtcgagggc ggccttgatc
     2821 ttcttcatca cctcgaagcc gccgcccatc tcctgcggga cgagctcgac gtcgcccgcc
     2881 cgggtcgcgc cgtggcaggc ggtgatcgag ccgccgtgct cgagcgcgac ctcggcgatc
     2941 tcccgcttgc actcgaccca gccccgccag ttctcctcgt cgagctcctg ctcccagatc
     3001 ccgatgtcga tctcggtcag gtagtcgccc cacggcttgt aggccgcgtt cgtgtaggcg
     3061 aacatccccc agtcgtcgaa gatcccgtag cggtcgatgt agcgctcggc gatcgcgtgc
     3121 cacttctccc gcaccgcggg gagctggctg tacgggatgg acgcgtcctc gcagtgccag
     3181 cacatcggaa cgacctgacc gtccttcagc cggccgtgga gcggcgtcgc gtagcggtcg
     3241 tgtcgcgagg cccagtcgcc ctgggagatc tcgtcgccga ggtacttgcc gcccgcctcc
     3301 ttggcgatgc gcatgagccg ccgcgacgcg ggctcgacct cgtcgcgcgc gccgtacatg
     3361 gcggaggcga tcaccgcccg cacggtcgga ggctgcggga tgtaggcctc gtcgtcgcgg
     3421 cggaggtacg cgagcttcca ctcgtcgaag aggacgacgc ccgcgagcgt cgcgagcccg
     3481 gacttcgcga gcgcgccggt cgcctcccag gcggtccggt agtcggagaa cgcgaagaag
     3541 gccgcgaact cgacctccgg gcgcgggacg agctcgagcg tcgcctcggt cgcgatcccg
     3601 agcgtgccct ggtggcccat gaacaggtgc ttgagctgga agccggtcga cgacttgcgc
     3661 accttccgcc cgccgccgtc gccgacctcg atcacctcgc ccgtcggcag gacgagctcc
     3721 atcgagatga cgaggtcgcg cgtgtgcccg tagcgcccgc cgatgagcga ccagccgctc
     3781 gtcccgatcc ggccgcccac gatcgagcac gggtacgagg ccgggtcgtc gggatagatg
     3841 acgccgtgcg ccttcagctc acggttgagc gtgagcatgt tgatccccgt ctgcaccgtg
     3901 accgtccggt cgacgaggtc gatgtcgagg atccggttca tgagcttcac gtcgacgacg
     3961 atcccgccgc ggagcggcac ggcgccgtcc gcgagccccg tcccgccggc gcgcgggacg
     4021 atcgggatcc cgtggcggtt cgcgagcttc acgacctcgg agacctgctc cgtcgtgcgc
     4081 gggaggacgg ccacgtccgg caggaactcg ccccagcggt gaacggggaa cggcgcgggc
     4141 acgcgcgtcc tgttcacccg cgcggggagg tcgggcagga cgtactcgtc tccgcccagg
     4201 atgccgcgca gctcgtcgag gacgccgtcg ccgagcgcgg tcgccgtcgc cgtcatgccg
     4261 cctcctcccg gtcgtcgagc ccggccgaga cggcgacgag ctccatcagg tccatgacct
     4321 cgagcgggcg ctcgcccgcc gcgccctgct cggagagcgg ccgctccgac cagacgcagg
     4381 cgctcacgag cgtgtcgacg tcgagctcgg ccgcccgctc cacgcggcgc ctgctgatct
     4441 ccgcggtcag gtcggggcgc tcgatcggca gcccgccgcc ggcgcccgag cagtacgacc
     4501 actgcgagac gtggtcgacg tccttgaacg tgatcccggg gatcgcgcgc aggatctcgc
     4561 gcggcgcctc gtggatgccc ttgcgcttgt tcagccggca ggcgtcgtgg tacgtgaccg
     4621 tccgctcgac cggccgggtg agcgcgagct tcccctcgcg gacgagctcc gcgacgagct
     4681 cgacgacgag gacgatctcg aagtcgaact cgtccccgaa gaagcgcgga tagtcctccg
     4741 tgaacgcgat gtagtcgtgc gggtcgagga cgaggatcct cctcgccccc acggcgcgcc
     4801 agtcggccac gttgtgctcg gcgaagcgcc gcgcctggtc gacgtacccc atctccgccg
     4861 ccgggccgcc gcagcaccac tgctcccgca tgagcccgaa ctcgacgccg cccgcctgga
     4921 ggatccgcgc gaccgcgcgc ggaagcgacg tgcggtggaa cgcggcctcg cagtcgcaga
     4981 acagcaccgt ctcgccgccg acgggcaggt cgaggcccgc ggcccagtcg gccacgtgct
     5041 cctgcgagac cggcacgtcg ccgagcaccg gctcgtttcg gcgctcgttc gtgagctcgt
     5101 tccagcgctt ccacgcgggc tgctcgaccc cctgctcgac ggcgagcgcg cgcatcgcct
     5161 tcacgacgtc gaccgtgcgc gtccggtagc ggtagaagtc gccggtgaag agcgtgttcg
     5221 ggcagcgcag ctcgcacgcg ccgcactggg tgcagtggac gtagtcgcgg gccgtgtcct
     5281 cgacggagac gaggccctgc tccatcgcga cgacgttcgc gtggaacgcg gtcggcgagt
     5341 ggttctcgtt ccgcgtgacc tgcatgaccg ggcagacctc gcggcagaac ttgtggcccg
     5401 agctgaagca gttgtacgcg gcgtttcgcc actcggcggc gaacgccgcc tcgcggccgt
     5461 ctcgcccgtt cgcgccctgc ccggttcccg tctctcgcac ctcgttctcg gccacggcgc
     5521 ctccctgggt cgcttccatc gggcgagccg cgccgtccct cggggctcgg cgcgggacgc
     5581 cgggctatcg tgaccgctgg gaggcgccga agcaagttcc ccgcgggcct cgccatttgg
     5641 cacgagacgt tgtagcctcc ggtgcagcca gcctccgtag gggaggtccc tgcagagggg
     5701 cagggagaac agagggaggg acaacatgct gggagggaac agcggccgcc ggcttacgct
     5761 cggtgtcctc gtgctcgcga tctcgctcgt cttcgggagc tcgcttgccg gggcacgaac
     5821 cgtcgagccg gacgacggcc tccaggatcg cgactcgatg ggaagctcga tggacccgag
     5881 cggcgcgctg agcgcgctcg acgccgagag cgacgtcgtg agctccggcc cgtccgcgaa
     5941 ggtgacgaag aacctcgcgg tcgcggggcg cggcgagcgg ctcatgccga acgcaaccac
     6001 ggacgtgtgg gcgcacgacg gctacgccta tctcgggacg ttcaacgagc cgtgcggcga
     6061 cggaaccggc gcgaacgggt cgggcgtcca catcttcgac gtctcgaacc cgaacaaggt
     6121 cacgccggcc gggcagctcc cgtccgtcct gggcagccgg atcaacgaca tcaaggtcgc
     6181 cgacatgaac agcggcacga tcctcgtcca ctcgaacgag tcgtgcggcg gccccgaggg
     6241 cgggttcgag gtgtacgaca tgagcaaccc gctcagcccg gtccacctcg cgagcgcgcg
     6301 cgtggacgag gcgaacgcca ccctgcgcga cctgttcggc ggcacgaacc tcggcgtgca
     6361 caacctgtac ctcttcaccc agggggaccg cgactacgtc gcgatgcaga cgcacgcgtg
     6421 gttcggcagc ttccagatct gggagctcac cgacccgtcg aacccgcagt tcgtgagcgc
     6481 ctggggcgcc gagctcctct gtgaggaggc gtactgcagc gacgacccgt acaacgagac
     6541 ggacgtgacg acgctcgtga accacatcaa cggctacatg ttcggcctgc tcacgccggc
     6601 gtacgggttc tcgcagaacc gcttcctgca cgacatgacg gtgacggccg acgggacgaa
     6661 ggcctacctc gcccactggg acgcgggcct
//
LOCUS       DASTFC010000054         7386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1006374,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000054 DASTFC010000000
VERSION     DASTFC010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006374"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2554)
                     /locus_tag="VFO88_01540"
     CDS             complement(<1..2554)
                     /locus_tag="VFO88_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9544239.1"
                     /translation="MDVIVTHTNTDFDAFASLLAARRLYPGAVAALSGSLNRNVREFY
                     RLHAEELDVAETGTVDHDAIERLIVVETVAAERLGEFEAVTRRPGVEVVAFDHHQPER
                     PDWIPEANLVTSDDGALTTTLVGIIAERELEPTPLEATVFALGIHEDTGSLTYPTVTQ
                     RDADALAWCLRHGARQDMIAQYLHTPLGEQERGLLDKLQESLEEHDVGGIPVLVAAVA
                     WPGYVDGVSNLVHKLVDLTDARGLVALVEMDERVVCVARARVAELDAAAVAGALGGGG
                     HPQAASAVFRGTVAEARELVLAALPRAVRRSLTAGQIMSRPARFVSPDDTVAHAMVEC
                     QRHRQSGILVGEPEDVVGLVEREDLDKAIGHELAHAPVKSVMAATVHACDADTPLAEV
                     QRLVVHAGASRVPVRRDGRVVGVVTRSDLLRALEEPVTEAAPARADLRERLHSLEGLQ
                     PAFEAIQAVGEGYEGVYLVGGAVRDVLMGEPTFDVDIAVEGDGIAFGRALAAALGGRA
                     VPHEKFGTAVVLAGDGTRVDVATTRTEFYDRPAALPRVEQATLRQDLFRRDFTINALA
                     VSLKGEDFGRVVDFFGGLDDLEAGVVRVIHPLSFIDDPTRIFRGIRYETRYGFRMDGH
                     TASLARACVEMNLVGELSSARLRDELQALLSEERAAEAVRRMADLGVAAAVHPRLAAD
                     EESVRLVGVSDELRARFTPEVPAWRARLAVLARPLSAHELFDWFERLRLRRRDAGLVA
                     DAVAVAPRLLELLAETDEAAEIRRLVEPHDPDGALLALALADEDGPARERLERYFREL
                     RGVSLEITGGDLAGLGVAESPRVGEILDELLRRKLNGELDGRSAELEAARELL"
     gene            complement(2733..3119)
                     /locus_tag="VFO88_01545"
     CDS             complement(2733..3119)
                     /locus_tag="VFO88_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET9544240.1"
                     /translation="MEKRVVHTDKAPAPFQGAPYSQGIVAGDFVFVSGQVAVDPATGD
                     VVEGGIAEQTERVMLNVAAVLEAAGSSTDKLVKCGIFLADFDDFQAMNEVYARHVGSS
                     PPARATVQVAYLPSGVLVEIDAVAHL"
     gene            complement(3130..4449)
                     /gene="murA"
                     /locus_tag="VFO88_01550"
     CDS             complement(3130..4449)
                     /gene="murA"
                     /locus_tag="VFO88_01550"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9544241.1"
                     /translation="MDSTLTDIIATDASSESFVIEGGRPLEGRIRSAGNKNAALPIVA
                     ACLLTDEPVLLTNVPAILDVATMLDLVQALGVHVERHGGGEVLIHAASEPGTELDEEL
                     CRQIRASILLAGPLLARAGEAIVPPPGGDVIGRRRVDVHIHALEQLGAVVEVNRRFTM
                     RTERLRGAPLFLDEPSVTGTENAIMAAVLAPGETVIGNAACEPHVQDLCRFLVALGAD
                     IEGIGSNVLRIGGVERLRGGEWRICPDHIEIASFVGLGAVTQGEIVIDDVVAEDLVAV
                     WPAFARLGVHATVEGNSVRVGRGDLRVEDDLGGQIPKIEDGPWPAFPADLTSIALAVA
                     TQCEGTILIFEKMFENRLFFVDKLVTMGARIILCDPHRAVVNGPARLYGERMESPDIR
                     AGMAMLIASLCAEGTSTIGNVGQIDRGYEKIDERLRALGAAIERVEA"
     gene            4499..5074
                     /locus_tag="VFO88_01555"
     CDS             4499..5074
                     /locus_tag="VFO88_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9544242.1"
                     /translation="MEVTRERAWETLTKYTKGEALRRHALAVEAAVGGYARERGEDEE
                     LWRAVALLHDFDYEIHPTLDRHPQDGAPILREEGWPEPVVEAVLSHAEHLSLPRDTPL
                     KKTLFACDELAGFVHACGLVRPDGIETLEPRSVKKKLKQPSFAAGVSRDDVRNGAEEL
                     GIDLDEHIANVIRFMRPVAGELGLRTEPAPA"
     gene            5071..5505
                     /locus_tag="VFO88_01560"
     CDS             5071..5505
                     /locus_tag="VFO88_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544243.1"
                     /translation="MKVTMLLADYAQVADGKLTIVGGGWSVTGPMPVPFAIAILFEVP
                     WDRANMKHRFRLDLVDSDGHVVFVPTDEGEEPLVIEGEFEAGRPPGLKPGTPLDVPIA
                     INLPGPPIQPGGRYEWRLTVNGEGGENWRLPFSTRGEAEQPA"
     gene            complement(5502..6593)
                     /locus_tag="VFO88_01565"
     CDS             complement(5502..6593)
                     /locus_tag="VFO88_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9544244.1"
                     /translation="MSGLERLRAEFPVLAHAAYLNAGTFGPLARRTVEAQERWLRRGL
                     EEGRAGHAFFVEVLGLRDDLRARLGALVGAPPEAMALTTSTTEGCNIVLAGLGLGPED
                     EIVTTDSEHPGLLGPLRTSGARVRVARVSEVPADGALELIERELTPATRLVALSHVLW
                     TTGHVIPVERLAGRGFRLLVDGAQSVGAIPVDVGSLGVDFYTVSGQKWLCGPDTTGAL
                     YVRPDERGGLAMRTASYLSWEDVHELEPWPGARRYESVWLPPGSVAGLLASLDLADEA
                     GEERFAAARATAERCRELVAGRADLVTEPGQATLVSFRPRGAEPAEVVERCAECAVVV
                     RELPGTGMVRASCGWWTSEDDLRRLVEAL"
     gene            complement(6590..>7386)
                     /locus_tag="VFO88_01570"
     CDS             complement(6590..>7386)
                     /locus_tag="VFO88_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9544245.1"
                     /translation="VLLLEEVELAIDEVGEGTAAEAHAHRRDYAAVSFVVRTWNLFHG
                     RTHPPSRRTHLRHMVELVTEDGPDVVALQEVPVWALGRLERWSGMTARWAVTVPALLL
                     GPLARLVTEIDAARLRSLFTGQANVVLVAPRLAVGGQRLLQLNDAGRWEWLRLGRQQR
                     WCQAVGVDELVVANVHATNEPKAAAAEAARAAAFLSGEERVVLCGDFNVSAHAVEGFS
                     PPIAGLDQILVRGLELVEPARAWEPGRRALGGRLLSDHAPVEAVVA"
BASE COUNT         1010 a         2777 c         2693 g          906 t
ORIGIN      
        1 cgaggagctc ccgggccgcc tcgagctcgg ccgagcggcc gtccagctcc ccgttcagct
       61 tgcgccggag cagctcgtcg aggatctccc cgacgcgcgg cgactcggcg acgccgagcc
      121 cggccaggtc gccgccggtg atctcgagcg agaccccgcg cagctcccgg aagtagcgct
      181 cgagccgttc gcgcgccggc ccgtcctcgt ccgcgagggc gagcgcgagg agcgccccgt
      241 ccgggtcgtg cggctcgacg agccggcgga tctcggccgc ctcgtccgtt tccgcgagga
      301 gctcgaggag gcgcggggcg acggcgacgg cgtcggcgac gagccccgcg tcgcggcgcc
      361 gcagccggag ccgctcgaac cagtcgaaga gctcgtgcgc ggagagcggc cgcgcgagca
      421 ccgcgaggcg cgcccgccac gccgggacct ccggcgtgaa gcgcgcgcgc agctcgtccg
      481 agacgccgac gagccggacg gactcctcgt cggccgcgag gcgcgggtgc acggcggcgg
      541 cgacgccgag gtcggccatt cgcctgaccg cctccgccgc gcgctcctcg gagagcagcg
      601 cctgcagctc gtcgcgcagg cgagccgacg acagctcgcc gacgaggttc atctcgacgc
      661 aggcgcgcgc gagcgaggcc gtgtgcccgt ccatccggaa cccgtagcgc gtctcgtagc
      721 ggatcccacg gaagatccgc gtcgggtcgt cgatgaagga cagcggatgg atgacccgca
      781 cgacgcccgc ctcgaggtcg tcgagcccgc cgaagaagtc gaccacgcgc ccgaagtcct
      841 cgcccttcag ggagacggcc agcgcgttga tcgtgaagtc gcgccggaag aggtcctggc
      901 ggagcgtcgc ctgctcgacc cgcgggagcg ccgccggccg gtcgtagaac tcggtgcggg
      961 tcgtggcgac gtcgacgcgc gtgccgtccc ccgcgagcac gaccgccgtg ccgaacttct
     1021 cgtgcggcac cgcgcggccg ccgagcgccg cggcgagcgc ccgtccgaac gcgatcccgt
     1081 cgccctcgac ggcgatgtcc acgtcgaagg tcggctcgcc catgagcacg tcgcggacgg
     1141 cgccgccgac gaggtagacg ccctcgtacc cctcccccac cgcctggatc gcctcgaacg
     1201 cgggctggag cccctcgagc gagtgcagcc gctcgcgcag gtcggcgcgc gcgggcgcag
     1261 cctccgtgac cggctcctcg agcgcgcgca ggaggtcgct gcgcgtgacg acgccgacga
     1321 cgcggccgtc gcggcgcacc ggaacgcgac tcgcgcccgc gtggacgacg agccgctgca
     1381 cctccgcgag cggcgtgtcc gcgtcgcagg cgtgaacggt cgcggccatc acgctcttca
     1441 ccggcgcgtg cgcgagctcg tggccgatcg ccttgtcgag atcctcgcgc tcgacgaggc
     1501 cgacgacgtc ctccggctcg ccgacgagga tcccgctctg gcgatggcgc tggcactcga
     1561 ccatcgcgtg cgcgaccgtg tcgtcgggcg agacgaaccg ggccgggcgc gacatgatct
     1621 ggccggcggt gagcgagcgc cgaaccgcgc ggggcagcgc ggcgaggacc agctcgcgcg
     1681 cctccgccac ggtgccgcgg aagacggccg aggccgcctg gggatgaccg ccaccgccga
     1741 gcgcgcccgc gaccgccgcc gcgtcgagct cggcgacgcg cgcgcgggcg acgcagacga
     1801 cccgttcgtc catctcgacg agcgcgacga gcccgcgcgc gtcggtcagg tcgacgagct
     1861 tgtggacgag gttcgagacg ccgtcgacgt agccgggcca cgcaaccgcc gcgacaagga
     1921 cggggatccc gcccacgtcg tgctcctcaa ggctttcctg gagcttgtcg aggagccccc
     1981 gctcctgctc gccgagcggc gtgtggaggt actgcgcgat catgtcctgc cgcgcgccgt
     2041 ggcgcaggca ccacgcgagc gcgtccgcgt ctcgctgcgt gacggtcggg tacgtgagcg
     2101 accctgtgtc ctcgtggatc ccgagcgcga acacggtcgc ctcgagcggc gtcggctcga
     2161 gctcgcgctc ggcgatgatc ccgacgaggg tcgtcgtcag cgcgccgtcg tcgctcgtga
     2221 cgaggttcgc ctcgggaatc cagtcgggcc tctccggctg gtggtggtcg aacgcgacca
     2281 cctcgacgcc gggccggcgc gtgacggcct cgaactcgcc gagccgttcg gccgcgaccg
     2341 tctcgacgac gatcagccgc tcgatcgcgt cgtggtcgac cgtgcccgtc tccgccacgt
     2401 cgagctcctc ggcgtggagg cggtagaact cccgcacgtt ccggttgagc gaccccgaga
     2461 gcgccgcgac cgcgcccggg tagagccgcc gggcggcgag aagcgacgcg aatgcgtcga
     2521 agtccgtgtt cgtgtgcgtg acgatgacgt ccacgcgacg aagcgtagtg cggcgggcgg
     2581 gccgaccctg cgccgcgtca ggttcacgca cgtgtagatc cgggtcccga ccctcccgcc
     2641 tgccgccgct caggctaacg cggaaatgcg cgcgcgtgtg cgcccgtacc gtgccgaatg
     2701 tgtgacgacc gacgagcgcc cggcgcggcg cgctacaggt gggcgaccgc gtcgatctcg
     2761 acgagaacgc cgctcgggag atacgcgacc tgcacggtcg cgcgcgcggg cggcgacgag
     2821 ccgacgtgcc gcgcgtagac ctcgttcatc gcctggaagt cgtcgaagtc ggcgaggaag
     2881 atcccgcact tcacgagctt gtccgtgctc gaccccgccg cctcgagcac cgccgcgacg
     2941 ttcagcatca cgcgctcggt ctgctcggcg atcccgccct cgacgacgtc tccggtcgcg
     3001 ggatcgacgg ccacctggcc ggagacgaac acgaagtcgc cggcgacgat gccctgcgag
     3061 tacggcgcgc cctggaacgg ggcgggcgcc ttgtccgtgt gtacgacgcg cttctccatg
     3121 cgggggatcc tacgcctcca cgcgctcgat cgcggcgccg agggcgcgca ggcgctcgtc
     3181 gatcttctcg tagccgcggt cgatctggcc gacgttcccg atcgtggacg tcccctcggc
     3241 gcagaggctc gcgatcagca tcgccatccc ggcgcggatg tccgggctct ccatccgctc
     3301 gccgtagagc cgcgccggcc cgttcacgac cgcgcgatgc gggtcgcaga ggatgatccg
     3361 ggcgcccatc gtgacgagct tgtcgacgaa gaagaggcgg ttctcgaaca tcttctcgaa
     3421 gatgaggatc gtcccctcgc actgggtcgc cacggcgagc gcgatcgacg tgaggtcggc
     3481 gggaaacgcg ggccacgggc cgtcctcgat cttcgggatc tggccgccga ggtcgtcctc
     3541 gacgcgcagg tcgccccggc ccacgcgcac ggagttgccc tcgaccgtcg cgtgcacgcc
     3601 gagccgcgcg aaggccggcc agacggcgac gagatcctcg gccacgacgt cgtcgatcac
     3661 gatctccccc tgcgtgacgg cgccgaggcc gacgaagctc gcgatctcga tgtgatccgg
     3721 gcagatgcgc cactcgccgc cgcgaagccg ctcgacgccc ccgatcctga ggacgttcga
     3781 gccgatgccc tcgatgtcgg cgccgagcgc gacgaggaag cggcagagat cctgcacgtg
     3841 cggctcgcag gcggcgttcc cgatcaccgt ctcgccgggc gcgaggacgg ccgccatgat
     3901 ggcgttctcg gtgcccgtca cggacggctc gtcgaggaag agcggtgccc cgcgcagccg
     3961 ctccgtccgc atcgtgaagc gccggttcac ctcgacgacc gcgccgagct gctcgagcgc
     4021 gtggatgtgg acgtcgacgc gccgccgccc gatcacgtcg ccgcccggag gcgggacgat
     4081 cgcctcgccc gcgcgggcga gaagcggccc ggcgaggagg atcgaggcgc ggatctgccg
     4141 gcagagctcc tcgtcgagct cggtgccggg ctcgctcgcg gcgtggatca gcacctcgcc
     4201 gccgccgtgg cgctcgacgt ggacgccgag cgcctgcacg aggtcgagca tcgtcgcgac
     4261 gtcgaggatc gcgggcacgt tcgtgaggag caccggctcg tccgtcagga ggcacgcggc
     4321 cacgatcggg agggcggcgt tcttgttccc ggcgctccgg atccgcccct cgagggggcg
     4381 gccgccctcg atgacgaagg actccgacga cgcgtcggtc gcgatgatgt cggtcagggt
     4441 gctgtccacg ggcccggcgg ccctcggtcg gccgcggcgg gctatcctac ccggcccgat
     4501 ggaagtgacg cgcgagcgcg cctgggagac cctgacgaag tacacgaagg gcgaggccct
     4561 gcgccgccac gcgctcgccg tcgaggcggc ggtcggcggc tacgcgcgcg agcggggcga
     4621 ggacgaagag ctctggcgcg cggtcgcgct cctgcacgac ttcgactacg aaatacaccc
     4681 gacgctcgac cggcatccgc aggacggcgc gccgatcctc cgcgaggagg gctggccgga
     4741 gcccgtcgtg gaggcagtcc tctcccacgc cgagcacctg tcgctcccgc gcgacacgcc
     4801 gctcaagaag acgctcttcg cgtgcgacga gctggccggc ttcgtccacg cgtgcgggct
     4861 cgtgcgcccg gacgggatcg agacgctcga gccccgctcc gtgaagaaga agctgaagca
     4921 gccgtccttc gccgccggcg tgagccgtga cgacgtccgc aacggcgccg aggagctcgg
     4981 gatcgacctc gacgagcaca tcgcgaacgt gatccggttc atgcggccgg tcgcgggcga
     5041 gctcggcctc cggacggagc cggcgcccgc gtgaaggtga cgatgctcct cgccgactac
     5101 gcgcaggtcg cggacggcaa gctcacgatc gtcggcggcg ggtggtcggt gaccgggccg
     5161 atgcccgtcc cgttcgcgat cgcgatcctc ttcgaggtgc cgtgggaccg cgcgaacatg
     5221 aagcaccggt tccggctcga cctcgtggac tcggacgggc acgtcgtctt cgtcccgacc
     5281 gacgagggcg aggagccgct cgtgatcgag ggcgagttcg aggcgggccg cccgccgggc
     5341 ctcaagccgg ggacgccgct cgacgtgccg atcgcgatca acctccccgg gccgccgatc
     5401 cagccgggcg ggcgctacga gtggcggctc acggtgaacg gcgagggcgg cgagaactgg
     5461 cggctgccct tctccacgcg cggcgaggcc gagcagcccg cctagagcgc ctcgacgagg
     5521 cggcgcaggt cgtcctcgct cgtccaccag ccgcacgacg cgcgcaccat cccggtcccc
     5581 ggcagctcgc ggacgacgac ggcgcactcc gcgcagcgct cgaccacctc ggccggctcc
     5641 gcgccgcgcg ggcggaagct cacgagcgtc gcctggccgg gctcggtcac gaggtcggcg
     5701 cgcccggcga cgagctcgcg gcaccgctcg gccgtcgccc gggccgccgc gaagcgctcc
     5761 tccccggcct cgtcggcgag gtcgagcgag gcgaggagcc ccgcgaccga gccgggcgga
     5821 agccagaccg actcgtagcg ccgcgcgccc ggccacggct cgagctcgtg cacgtcctcc
     5881 cacgagaggt acgaggccgt gcgcatggcg agcccgcccc gctcgtcggg ccggacgtag
     5941 agcgcccccg tcgtgtccgg cccgcagagc cacttctgac cggacacggt gtagaagtcg
     6001 accccgagcg agcccacgtc gacggggatc gcgcccacgc tctgcgcccc gtccacgagc
     6061 aggcggaagc cgcgcccggc gagccgctcg accgggatga cgtggccggt cgtccagagg
     6121 acgtgtgaga gcgcgacgag ccgggtcgcc ggcgtgagct cccgctcgat cagctcgagc
     6181 gccccgtccg ccggaacctc ggacacgcgc gcgacgcgca cgcgtgcgcc ggacgtccgg
     6241 agcggcccga ggaggcccgg gtgctcggag tccgtcgtca cgatctcgtc ctccggcccg
     6301 agaccgagcc ccgctaggac gatgttgcag ccctccgtcg tcgaggtcgt gagcgccatc
     6361 gcctcgggcg gcgcccccac gagcgccccg agccgggccc gcaggtcgtc gcgcaggccg
     6421 agcacctcga cgaagaacgc gtggcccgcg cggccctcct ccaggccgcg gcggagccac
     6481 cgctcctgcg cctcgaccgt ccggcgcgca aggggcccga acgtgccggc gttcaggtac
     6541 gcggcgtgcg cgagcaccgg gaactcggcc cggagccgct cgaggccgct cacgcgacca
     6601 ccgcctcgac gggcgcgtgg tccgagagca gccggccccc gagcgcacgg cgtcccggct
     6661 cccaggcgcg ggccggctcg acgagctcga ggccgcggac gaggatctgg tcgagccccg
     6721 cgatcggcgg cgagaacccc tcgaccgcgt gcgccgacac gttgaagtcg ccgcagagca
     6781 cgacgcgttc ctccccggag aggaacgccg ccgcgcgtgc ggcctccgct gcggccgcct
     6841 tcggctcgtt cgtcgcgtgg acgttggcga cgacgagctc gtccacgccg accgcctggc
     6901 accaccgctg ctgccggccg aggcgcagcc actcccaccg cccggcgtcg ttcagctgca
     6961 ggagccgctg cccgccgacg gcgagccgcg gcgcgacgag gacgacgttc gcctgcccgg
     7021 tgaagagcga ccgcaggcgg gcggcgtcga tctcggtcac gaggcgcgcg agcggcccga
     7081 ggaggagcgc cggcacggtg accgcccagc gcgccgtcat cccgctccag cgctcgagcc
     7141 ggccgagcgc ccagacgggc acctcctgga gcgcgacgac gtccggcccg tcctccgtca
     7201 cgagctcgac catgtgccgg aggtgcgtcc gccgcgacgg cgggtgggtg cggccgtgaa
     7261 agaggttcca ggtgcggacg acgaacgaca cggccgcgta gtctcgccga tgcgcatgcg
     7321 cttccgccgc cgtcccttcg ccgacctcgt cgatcgccag ctcgacctct tcgaggagga
     7381 gcacgc
//
LOCUS       DASTFC010000055         9795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1286588,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000055 DASTFC010000000
VERSION     DASTFC010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286588"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VFO88_01575"
     CDS             complement(<1..1088)
                     /locus_tag="VFO88_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9544246.1"
                     /translation="MAVVIGTNLRKDLSGEALFDGVSFKVERRDRVALAGPNGAGKTT
                     LLRMIAGETSVDGGTLVLSKGARVALHDQRPPLERGLTLREYVLAGAADLVAIEGELG
                     RLERAMADGAHDAQTLARYAEAQARLEHAGGYAWRDRATAVVRGLGFADADLDRRLET
                     FSGGELTRASLARSLGGDPDLLLLDEPTNHLDVASLEWLERELTSLDAAVILVAHDRW
                     FLEAVTSATLELAAGRATYFPGPWHAWRREKAERAAHAQKTADRVAADIERLDRFVQR
                     FRYKKTKAKQAQAKLTQISRLEKEKAEARGEVELLTRRTRSLGFEFLKPPRSGRTVLE
                     VQGVRLEAGDKHLLDDASFALERGEHVGL"
     gene            complement(1135..2985)
                     /locus_tag="VFO88_01580"
     CDS             complement(1135..2985)
                     /locus_tag="VFO88_01580"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET9544247.1"
                     /translation="MSDSDRWRELGQQLRVDSIRASAVTKSGHPTSSMSAADLVAVLA
                     DRYLTYDFDRPDDPRNDHLVFSKGHASPLLYSLYKAAGAVTDDELLTFRKLGSRLEGH
                     PTPVIPWVDVATGSLGQGLPIGVGIALAGKKLDRLPYRVWVLCGDSELAEGSMWEAFE
                     HASFYELDNLVAVLDINRLGQTGETMHGWDLDLYARRAEAFGWHTITIDGHDVEAIDR
                     AYAEALETTGRPTAIVARTMKGKGAESVENKPGAHGKALDDPEIAIAELGGARDLRIE
                     VRRPDHDAEPHRFDTGPLELPTYEVGSEVATRKAYGEALAALGSADGLVVALDGEVSN
                     STYSEIFREAHPERYLEMYIAEQQMIAAAVGIQVRGWRPFSSTFAAFLTRAYDFIRMA
                     AVSRATMALCGSHAGVSIGEDGPSQMALEDLASLRAVGGSTVLYPCDGNQTAKLVAAM
                     AELDGISYLRTTRAATPVLYGPDEDFPVGGSKVVRSSDADDVAIVGAGITVREALEAA
                     DALAEDGINARVIDAYSVKPIDAETLHAAAEATGGRIVTVEDHWPEGGLGEAVLSAFA
                     DAEERPRVKLLAVQGMPTSATPDELLREAGIDADAIAAAARALVGTPVAR"
     gene            3219..3677
                     /gene="rpiB"
                     /locus_tag="VFO88_01585"
     CDS             3219..3677
                     /gene="rpiB"
                     /locus_tag="VFO88_01585"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9544248.1"
                     /translation="MKVAVAFDHRGTKLRERVLADLVALGHETVDLGTDAPEPRVDYP
                     DKARELAAAIRSGEAERGILVCGSGVGASVAANKIPGIRAAICHDTYSAHQGVEHDDM
                     NVLCLGSEIVGGELAGELVRAFLAAEFDGGERYVARLQKIEEMEAAHTHG"
     gene            3670..4779
                     /gene="tal"
                     /locus_tag="VFO88_01590"
     CDS             3670..4779
                     /gene="tal"
                     /locus_tag="VFO88_01590"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET9544249.1"
                     /translation="MADTRLHSLAEHGQSPWLDFVSRNMIRTGDLERRIHDDAITGVT
                     SNPTIFQGAISAGNAYDDQLRELLAEEDDPRTLFFRLAARDIADACDLLRAAWEQGAR
                     GRDGHVSLEVDPGLAHDTQATIDQAQFLSDLVERPNLYVKIPATREGLPAIEEMISRG
                     VPINVTLIFSLERYKEVTEAYIRGVERFVEGGGDASRVTSVASFFVSRVDTEGDRRLD
                     ELGGHDELKGKLAIANAKLAYQEYRKIFAGERWERLAAKGAIPQYCLWASTSTKNPEY
                     RDVMYVEELIGRDTVNTMPEETIQAFQDHGRVEPTLEQGLDEARALFDRLAEAGVDYD
                     DVTETLEREGVKKFSDSFDELLEGIRATSRELVTA"
     gene            4783..6150
                     /locus_tag="VFO88_01595"
     CDS             4783..6150
                     /locus_tag="VFO88_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544250.1"
                     /translation="MAVGTAELVERIWARDASLWTGADEDRWLGWLDEPARQRGNVQE
                     LEAFAAEVREAGLTHTVLAGMGGSSLAPEVMRRTFGADAFTVCDTTHPDALRTLERGL
                     DRERTLVLVASKSGSTLETRCHLDWLWELLGGRGDRFVAITDPGSELEALAAERGFRR
                     VFHGEPEIGGRYSALSAFGLVPAALMGVDLARLLDRTLEMVEACRLEEGNPGLELGER
                     LGHGWLDGRDKVLVNPTPHGFGLWVEQLIAESTGKQGRGLVPVPGERSEGDDRLDAEV
                     RLNDAYELGQELFRWEVATAVAGALIGINPFDQPDVQAAKDRTTALLEAGGEPDVAPE
                     GSLDELLGEAQAGDYFCLQAFVEPTTGAERRIAETLDRVRAATGIVATAGFGPRYLHS
                     TGQLHKGGPASGLFLQVVDDPEELPVPGRDFGFRRLIHAQAAGDLDALRERGRRVARI
                     RWEDL"
     gene            6153..7052
                     /gene="gnd"
                     /locus_tag="VFO88_01600"
     CDS             6153..7052
                     /gene="gnd"
                     /locus_tag="VFO88_01600"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET9544251.1"
                     /translation="MQIGMVGLGRMGGNMTKRLEQDGHDVKTYDHGGGGTAGSLAELV
                     EQLDAPRAVWLMIPAGDPTEQAVDELLGLLAEGDVIVDGGNSNFRDSQRRAAKAGEHG
                     IGYVDAGVSGGIWGLEIGYCLMVGGEDDAVARIRPACETLAPEDGFAHVGPSGAGHYV
                     KMVHNGIEYGLMQAYAEGFELMYHSEFPLDLREISGIWRYGSVVRSWLLELLHEAFEQ
                     QGSRLEEIEGYVEDSGEGRWTITEAIDTAVPTPVIAAALFARFSSRQDINFAAKVAAA
                     LRNQFGGHAVRAVEEAKAHPDPR"
     gene            7056..8582
                     /gene="zwf"
                     /locus_tag="VFO88_01605"
     CDS             7056..8582
                     /gene="zwf"
                     /locus_tag="VFO88_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9544252.1"
                     /translation="MTETHAPPAAEQENPLLEGLRRRRTPEPCALTIFGASGDLTQRK
                     LFPALYALAVRHLLPEHFAVVGVARTQMTTEAFVERMRDAVQEHGRDEFHQETWEELA
                     AGVRYVAMEFDDDAAWEGLAAALTELDEERGTDGNRIHYLAVPPKAFEEIVRQVGKRR
                     TTSGWTRLICEKPFGHDLASARALNATIARYFAEEEVFRIDHYLGKETVQNMLVLRFA
                     NGIFEPIWNRQFVDHVQITVGESLGVEGRAGFYESAGAIRDIVQNHLLQLVALTAMEP
                     PIAFDPESVRNEKVKVLRSLHTPGPKHIVRGQYGPGDIEGAEVPGYREEEGVAPDSET
                     ETYVAGKLYVDNWRWADTPFYFRTGKRLPRRETTIAIQFKRAPKPPFDAAEEGMRPNV
                     LLLHIQPDEGVSLAVGAKVPGQGLAIRTVHMDFLYGGAFRTGLPEAYERLVLDCMLGD
                     ATLFMRADEVEEQWALVDSIIATWQRDRPAFPNYPSGSWGPAAADELLHRDGRSWRRH
                     "
     gene            8567..9445
                     /locus_tag="VFO88_01610"
     CDS             8567..9445
                     /locus_tag="VFO88_01610"
                     /inference="COORDINATES: protein motif:HMM:NF021632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET9544253.1"
                     /translation="MEAPLIRGATIRDVERELARLRNEQTGEDVSPSLRTSVLTHMAW
                     VPERWADAATRALGGLEERHPSRTILLYPRPDEERDELDASVDLRCFAVGGGAGVCFE
                     VVELALCGPRAQRPASVVAPLLVPDLPAFLRWRGDLPFGEPELEGLVGVADRLLVDSA
                     EWDDCTAGLERLETLFDRISVSDIAWSRSEPWRFELARRWPGIAEAETLAVAGPEADA
                     RLLAGWLRARLDREIELEHEPADTIERVAVDGEPVKLTRPDARTPSDLLSEQLDIFSR
                     DRVYEEAVCSYSSVPT"
     gene            9418..>9795
                     /locus_tag="VFO88_01615"
     CDS             9418..>9795
                     /locus_tag="VFO88_01615"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9544254.1"
                     /translation="MQLLVGSDLAELAAGWIAGRASAFSPFRVALAGGGTPERVYERL
                     AGLDLDWGSWHVWWSDEREVPPDHEDSNERMARRALLDRVPVPEEQIHPLHSVDVELP
                     DRFDLVLLGVGPDGHCASLFPGDP"
BASE COUNT         1342 a         3543 c         3576 g         1334 t
ORIGIN      
        1 agcccgacgt gctcgccccg ctcgagcgcg aacgacgcgt cgtcgagcag atgcttgtcg
       61 ccggcctcga ggcgcacgcc ctgcacctcg agcaccgtgc gcccgctgcg aggcggcttc
      121 aggaactcga acccgagcga gcgcgtccgg cgcgtgagga gctcgacctc gccgcgcgcc
      181 tcggccttct ccttctccag gcgggagatc tgcgtgagct tggcctgcgc ctgcttggcc
      241 ttcgtcttct tgtagcggaa gcgctgcacg aagcggtcga gccgctcgat gtcggcggcg
      301 acgcggtcgg ccgtcttctg cgcgtgggcg gcgcgctcgg ccttctcgcg gcgccaggcg
      361 tgccacgggc cggggaagta cgtcgcgcgg ccggcggcga gctcgagcgt ggcgctcgtc
      421 accgcctcga ggaaccagcg atcgtgcgcg acgaggatca ccgcggcgtc gagcgaggtg
      481 agctcccgct cgagccactc gaggctcgcg acgtcgaggt ggttcgtcgg ctcgtcgagc
      541 agcagcaggt cggggtcgcc gccgagcgac cgggcgagcg aggcgcgggt cagctcgccg
      601 ccggagaagg tctcgagccg ccggtccagg tcggcgtccg cgaagccgag cccccggacg
      661 acggcggtcg cccggtcgcg ccacgcgtac cctcccgcgt gctcgaggcg cgcctgcgcc
      721 tccgcgtagc gggcgagggt ctgcgcgtcg tgcgccccgt cggccatcgc gcgctcgagc
      781 cgcccgagct ctccctcgat cgcgacgagg tcggcggcgc cggcgaggac gtactcgcgc
      841 agcgtgagcc cccgctcgag cggcgggcgc tggtcgtgga gcgcgacgcg cgcgcccttc
      901 gagagcacga gcgtgccccc gtcgacgctc gtctcgccgg cgatcatccg gagcagcgtc
      961 gtcttccccg cgccgttcgg ccccgcgagc gccacccggt cgcgccgctc gaccttgaac
     1021 gagaccccgt cgaagagcgc ctcgcccgag agatccttgc ggagattcgt gccgatcacg
     1081 acggccatgc cgacatggtg gcacctggag gcacgggcag tcggcgaaaa ggaactagcg
     1141 cgcgaccggc gtgccgacga gcgcgcgcgc cgcggccgcg atcgcgtcgg cgtcgatccc
     1201 ggcctcgcgg aggagctcgt cgggcgtcgc ggaggtcggc attccctgca cggcgaggag
     1261 cttcacgcgc ggccgctcct cggcgtcggc gaacgccgag agcaccgcct cgccgagccc
     1321 gccctcgggc cagtggtcct cgaccgtgac gatccgcccg ccggtcgcct ccgcggccgc
     1381 gtggagggtc tccgcgtcga tcggcttcac cgagtaggcg tcgatcacgc gggcgttgat
     1441 cccgtcctcg gcgagcgcgt ccgccgcctc gagcgcctcg cgcacggtga tcccggcgcc
     1501 gacgatcgcg acgtcgtcgg cgtcggagga gcgcacgacc ttcgagccgc cgaccgggaa
     1561 gtcctcgtcc ggcccgtaga gcaccggcgt cgccgcgcgg gtcgtgcgga ggtacgagat
     1621 cccgtcgagc tcggccatgg cggcgacgag cttcgccgtc tggttcccgt cgcaggggta
     1681 gaggacggtc gagccgccga ccgcgcggag cgacgcgagg tcctcgagcg ccatctgcga
     1741 cggcccgtcc tcgccgatcg agacgcccgc gtgcgagccg cagagcgcca tcgtcgcgcg
     1801 gctcacggcc gccatgcgga tgaagtcgta cgcccgcgtc aggaacgcgg cgaacgtgga
     1861 cgagaagggc cgccagccgc gcacctggat cccgaccgcc gccgcgatca tctgctgctc
     1921 ggcgatgtac atctcgagat agcgctcggg gtgcgcctcg cggaagatct ccgagtacgt
     1981 cgagttcgag acctcgccgt cgagcgcgac gacgagcccg tcggccgagc cgagcgctgc
     2041 gagcgcctcg ccgtaggcct tgcgcgtcgc gacctcgctc ccgacctcgt acgtcggcag
     2101 ctcgagcggg cccgtgtcga agcggtgcgg ctctgcgtcg tggtcgggcc ttcgcacctc
     2161 gatccgcagg tcgcgcgcgc cgccgagctc ggcgatcgcg atctcagggt cgtccagcgc
     2221 cttcccgtgc gcgccgggct tgttctcgac cgactcggcg cccttgccct tcatcgtcct
     2281 cgcgacgatc gcggtcgggc gcccggtggt ctcgagcgcc tcggcgtacg cgcggtcgat
     2341 cgcctcgacg tcgtgcccgt cgatcgtgat cgtgtgccag ccgaaggcct ccgcgcgccg
     2401 cgcgtagagg tcgaggtccc agccgtgcat cgtctcgccc gtctgcccga gccggttgat
     2461 gtcgaggacc gcgacgaggt tgtcgagctc gtagaacgac gcgtgctcga aggcctccca
     2521 catcgagccc tcggcgagct ccgagtcgcc gcagagcacc cacacgcggt acgggagccg
     2581 gtcgagcttc ttgccggcga gcgcgatccc gaccccgatc ggcaggcctt ggccgagcga
     2641 gccggtggcg acgtcgaccc acgggatgac cggggtggga tgcccctcga gccggctgcc
     2701 gagcttgcgg aaggtcagga gctcgtcgtc ggtcaccgcg ccggcggcct tgtagagcga
     2761 gtagaggagc ggcgaggcgt gccccttcga gaagacgaga tggtcgttcc gggggtcgtc
     2821 gggccggtcg aagtcgtacg tcaggtaccg gtcggcgagc acggcgacga ggtcggccgc
     2881 cgacatggac gaggtcgggt ggcccgactt cgtgacggcg ctcgcgcgga tggagtcgac
     2941 gcggagctgc tgccccagct cgcgccagcg gtcggagtcg gacatggccg tcgcctttcc
     3001 gtcgggggga actcgaaacc ggcggccatt ctagggtggc ggcggcccgc ggcgtgcgcg
     3061 ggatcggcac ggactcgtga cgacgtcggc acgaagcgtc acagacgcgt gcggatttcc
     3121 tcgatacgct ttcactagtg ggtggcgggg gtcttccctc atcccgcgtg cgcgtgtttg
     3181 tcgggaacgg ggccggtgta ccctccctag gaggctctgt gaaggtcgcc gtcgcattcg
     3241 atcaccgagg cacgaagctg cgcgagcggg tgctcgccga cctcgtcgcg ctcgggcacg
     3301 agacggtcga cctcggcacc gacgcgcccg agccgcgcgt cgactacccc gacaaggccc
     3361 gcgagctcgc cgctgcgatc cgctccggcg aggccgagcg cgggatcctc gtctgcggct
     3421 ccggcgtcgg ggcctccgtc gccgcgaaca agatccccgg gatccgcgcg gcgatctgcc
     3481 acgacacgta ttccgcccac cagggcgttg agcacgacga catgaacgtc ctctgcctcg
     3541 ggtcggagat cgtcggcggg gagctcgccg gcgagctcgt ccgcgcgttt ctcgcggcgg
     3601 agttcgacgg gggcgagcgc tacgtcgcac gcctacagaa gatcgaagag atggaggcag
     3661 cacacacgca tggctgacac ccgacttcac tccctcgccg agcacggtca gagcccctgg
     3721 ctcgacttcg tctcgcgcaa catgatccga accggcgacc tcgagcgccg catccacgac
     3781 gacgcgatca ccggcgtcac ctcgaatccg accatcttcc agggcgcgat ctccgcgggg
     3841 aacgcgtacg acgaccagct gcgcgagctc ctggccgagg aggacgaccc gcgcacgctg
     3901 ttcttccggc tcgccgcgcg cgacatcgcg gacgcctgcg acctcctccg cgcggcctgg
     3961 gagcagggcg ccaggggccg cgacggccac gtctcgctcg aggtcgaccc cggcctcgcg
     4021 cacgacacgc aggcgacgat cgaccaggcg cagttcctga gcgacctcgt cgagcgcccg
     4081 aacctctacg tgaagatccc ggcgacccgt gagggcctgc cggcgatcga ggagatgatc
     4141 tcccgcggcg tcccgatcaa cgtgacgctc atcttctcgc tcgagcgcta caaggaggtc
     4201 acggaggcgt acatccgcgg cgtcgagcgc ttcgtcgagg gcggcggcga cgcgagcagg
     4261 gtcacgtcgg tcgcgagctt cttcgtctcg cgcgtcgaca cggagggcga ccggcgcctg
     4321 gacgagctcg gcggtcacga cgagctcaag ggcaagctcg caatcgcgaa cgcgaagctc
     4381 gcgtaccagg agtacaggaa gatcttcgcc ggggagcgct gggagcgcct ggccgcgaag
     4441 ggcgcgatcc cgcagtactg cctgtgggcc tcgacctcga cgaagaaccc ggagtaccgg
     4501 gacgtcatgt acgtcgagga gctgatcggc cgcgacaccg tgaacacgat gccggaggag
     4561 acgatccagg cgttccagga tcacgggcgg gtcgagccga cgctcgagca ggggctcgac
     4621 gaggcgcgcg cgctcttcga ccggctcgcc gaggcgggcg tcgactacga cgacgtgacc
     4681 gagacgctcg agcgcgaggg cgtgaagaag ttctccgact cgttcgacga gctgctcgag
     4741 gggatccggg cgacgagccg cgagctcgtg acggcgtagc ccatggcggt cgggaccgcc
     4801 gagctcgtcg agcgcatctg ggcgcgcgac gcctcgctct ggacgggcgc cgacgaggac
     4861 cgctggctcg gctggctcga cgagccggcg cggcagcgcg ggaacgtcca ggagctcgag
     4921 gctttcgccg ccgaggtgcg cgaggccggc ctcacgcaca cggtcctcgc cggcatgggc
     4981 ggctcgagcc tcgcgcccga ggtcatgcgg cgcacgttcg gggccgacgc cttcaccgtc
     5041 tgcgacacga cgcatccgga cgccctccgg acgctcgagc gcggcctcga tcgcgagcgc
     5101 acgctcgtcc tcgtcgcctc gaagtccggc tcgacgctcg agacgcgctg ccacctcgac
     5161 tggctctggg agctcctcgg cgggcgcggc gaccgcttcg tcgcgatcac cgaccccggc
     5221 tccgagctcg aggcgctcgc ggccgagcgc ggcttccgcc gagtcttcca cggcgagccg
     5281 gagatcggcg ggcgctactc ggcgctctcc gccttcgggc tcgttccggc cgcgctcatg
     5341 ggcgtcgacc tcgcgcggct cctcgaccgg acgctcgaga tggtcgaggc ctgccgcctc
     5401 gaggagggca acccggggct cgagctcggc gagcggctcg ggcacggctg gctcgacggg
     5461 cgcgacaagg tgctcgtcaa cccgaccccg cacggcttcg ggctctgggt cgagcagctg
     5521 atcgccgagt cgaccggtaa gcaggggcgc gggctcgtgc ccgttcccgg cgagcggagc
     5581 gagggcgacg accggctgga cgccgaggtg cgcctgaacg acgcgtacga gctcgggcag
     5641 gagctcttcc gctgggaggt cgcgacggcc gtcgccggcg cgctcatcgg catcaacccg
     5701 ttcgaccagc cggacgtgca ggcggcgaag gaccggacga ccgcgctcct cgaggcgggc
     5761 ggcgagcccg acgtcgcgcc ggagggatcg ctcgacgagc tcctcggcga ggcgcaggcg
     5821 ggtgactact tctgcctcca ggccttcgtc gagccgacca ccggggcgga gcgccgcatc
     5881 gccgagacgc tcgaccgggt ccgcgccgcg acgggcatcg tcgcgaccgc gggcttcggg
     5941 ccgcgctacc tccactcgac gggccagctg cacaagggtg ggccggcgag cgggctcttc
     6001 ctgcaggtcg tggacgaccc cgaggagctg ccggttcccg gccgcgactt cggctttcgc
     6061 cgcctgatcc acgcgcaggc ggcgggcgac ctcgacgcct tgagagagcg cggccgccgt
     6121 gtcgcgcgca tccgatggga ggacctctag cgatgcagat cgggatggtc ggcctcggcc
     6181 gcatgggcgg caacatgacg aagcgcctcg agcaggacgg gcacgacgtg aagacgtacg
     6241 accacggcgg cgggggcacc gcggggtcgc tcgccgagct cgtggagcag ctcgacgcgc
     6301 cgcgtgccgt ctggctcatg atcccggccg gcgacccgac cgagcaggcc gtcgacgagc
     6361 tgctcggcct cctcgccgag ggcgacgtga tcgtcgacgg cggcaactcg aacttccgcg
     6421 actcccagcg ccgcgccgcc aaggccggcg agcacgggat cggctacgtc gacgcgggcg
     6481 tctcgggcgg catctggggg ctcgagatcg gctactgcct catggtcggc ggcgaggacg
     6541 acgcggtcgc gcgcatccgc cccgcctgcg agacgctcgc ccccgaggac ggcttcgcgc
     6601 acgtcggccc gtccggcgcg gggcactacg tgaagatggt gcacaacggg atcgagtacg
     6661 gcctcatgca ggcctatgcc gaggggttcg agctcatgta ccactcggag ttcccgctcg
     6721 acctccgcga gatctccggg atctggcgct acgggtcggt cgtgcgctcg tggctgctcg
     6781 agctgctcca cgaggcgttc gagcagcagg gctcgcggct cgaggagatc gagggctacg
     6841 tcgaggactc cggggagggc cgctggacga tcaccgaggc gatcgacacc gccgtgccga
     6901 cgcccgtgat cgctgccgcg ctcttcgccc gcttctcgtc gcgccaggac atcaacttcg
     6961 ccgcgaaggt cgcggccgcg ctccgcaacc agttcggcgg ccacgccgtc cgcgcggtcg
     7021 aagaggcgaa ggcgcacccg gacccgaggt agcgcgtgac cgagacgcac gccccgcccg
     7081 ccgccgagca ggagaacccc ctcctcgagg gcctccgccg ccgccggacg cccgagccgt
     7141 gcgcgctcac gatcttcggc gcctcgggcg acctcacgca gcgcaagctc ttccccgcgc
     7201 tctacgcgct cgcggtgcgc cacctcctgc cggagcactt cgccgtcgtc ggcgtcgcgc
     7261 ggacgcagat gacgacggag gcgttcgtcg agcggatgcg ggacgcggtg caggagcacg
     7321 gccgcgacga gttccaccag gagacctggg aggagctcgc ggccggcgtc cgctacgtcg
     7381 cgatggagtt cgacgacgac gccgcctggg aggggctcgc cgccgccctc accgagctcg
     7441 acgaggagcg cggcacggac ggcaaccgca tccactacct cgccgtgccg cccaaggcgt
     7501 tcgaggagat cgtccgccag gtcggcaagc ggcggacgac ctcggggtgg acgcggctca
     7561 tctgtgagaa gccgttcggc cacgacctcg cgagcgcgcg ggcgctcaac gcgacgatcg
     7621 cccgctactt cgccgaggag gaggtcttcc ggatcgacca ctacctcggc aaggagacgg
     7681 tccagaacat gctcgtcctg cgcttcgcga acgggatctt cgagccgatc tggaaccgcc
     7741 agttcgtcga ccacgtgcag atcacggtcg gcgagtcgct cggcgtcgag gggcgcgcgg
     7801 ggttctacga gtcggccggc gcgatccgcg acatcgtcca gaaccacctc cttcagctcg
     7861 tcgcgctgac ggcgatggag ccgccgatcg ccttcgaccc cgagtcggtg cggaacgaga
     7921 aggtgaaggt gctccgctcg ctccacacgc cggggccgaa gcacatcgtg cgcgggcagt
     7981 acgggccggg cgacatcgag ggcgccgagg tgccggggta ccgggaggag gagggcgtcg
     8041 cgcccgactc cgagaccgag acgtacgtgg ccggcaagct ctacgtggac aactggcgct
     8101 gggccgacac gccgttctac ttccggacgg gcaagcggct gccgcggcgc gagacgacga
     8161 tcgcgatcca gttcaagcgc gcgccgaagc cgccgttcga cgcggccgag gagggcatgc
     8221 ggccgaacgt cctgctcctg cacatccagc cggacgaggg cgtctcgctc gcggtcggcg
     8281 ccaaggtgcc gggccagggg ctggcgatcc ggacggtgca catggacttc ctctacggcg
     8341 gcgcgttccg gaccggcctg ccggaggcgt acgagcggct ggtcctcgac tgcatgctcg
     8401 gcgacgcgac gctcttcatg cgggcggacg aggtcgagga gcagtgggcg ctcgtcgact
     8461 cgatcatcgc gacctggcag cgcgatcggc ccgcgttccc gaactacccg tccggttcgt
     8521 gggggcccgc ggccgcggac gagctgctcc acagggacgg gcgctcgtgg aggcgccatt
     8581 gatccgcggc gccaccatcc gcgacgtcga gcgggagctc gcgcggctgc gaaacgagca
     8641 gaccggcgag gacgtctcgc cgagcctccg cacgagcgtg ctgacgcaca tggcatgggt
     8701 gccggagcgc tgggcggacg cggcgacgcg cgcgctcggc gggctcgagg agcggcaccc
     8761 gtcccggacg atcctcctct acccgcgccc ggacgaggag cgcgacgagc tcgacgcgtc
     8821 cgtcgacctg cgctgcttcg ccgtcggcgg cggtgccggc gtctgcttcg aggtggtcga
     8881 gctcgccctc tgcggcccgc gggcccagcg gcccgcgagc gtcgtcgcgc ccctcctcgt
     8941 gccggatctc ccggccttcc tgcgctggcg cggcgacctg ccgttcggcg agccggagct
     9001 cgaggggctc gtcggcgtcg ccgaccggct cctcgtcgac tcggccgagt gggacgactg
     9061 cacggcgggc ctcgagcgcc tcgagacgct cttcgaccgg atcagcgtct cggacatcgc
     9121 ctggtcgcgg tccgagccgt ggcgcttcga gctcgcgcgc cgctggccgg ggatcgccga
     9181 ggcggagacc ctcgcggtcg ccgggcccga ggccgacgcg cgcctcctcg ccggctggct
     9241 gcgggcgcgg ctcgaccgcg agatcgagct ggagcacgag ccggcggaca cgatcgagcg
     9301 cgtggcggtc gacggcgagc ccgtgaagct cacccgtccc gatgcgcgca cgcccagcga
     9361 cctgctctcg gagcagctcg acatcttctc gcgcgaccgg gtctacgagg aggccgtgtg
     9421 cagctactcg tcggttccga cctagccgag ctcgccgccg gctggatcgc gggccgcgcc
     9481 tcggcgttct cgccgttccg ggtcgccctc gcgggcggcg gcacgccgga gcgcgtctac
     9541 gagcggctcg ccgggctcga cctcgactgg ggctcgtggc acgtctggtg gagcgacgag
     9601 cgcgaggtgc cgcccgacca cgaggactcg aacgagcgca tggcccgccg cgccctcctc
     9661 gaccgggtgc cggttcccga ggagcagatc catccgctcc actcggtcga cgtcgagctg
     9721 cccgaccggt tcgacctcgt cctgctcggc gtcggcccgg acgggcactg cgcctcgctc
     9781 ttccccggcg acccc
//
LOCUS       DASTFC010000056         4831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_231777,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000056 DASTFC010000000
VERSION     DASTFC010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231777"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFO88_01620"
     CDS             <1..649
                     /locus_tag="VFO88_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET9544255.1"
                     /translation="GGEIPLERRELTLVLRPRQISGFGGCNEFGARARIAGERIRVRG
                     PLTSTLMGCGAEINAVEEAYFDALRDVERRELKDGRLVLTGPETELVFEPLAPVDPER
                     LDGRTWTLESWTVDGATRTARARPATLRFGRDGRVEAHTRCRDLAGRYILQGHEVVLT
                     EAEMSRRDHAGCSREAIEQEGAVVGALGDRFRVDVDGDRLRIESRDGYVLVLRAD"
     gene            744..953
                     /locus_tag="VFO88_01625"
     CDS             744..953
                     /locus_tag="VFO88_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544256.1"
                     /translation="MMPTAIADRPTFDPLSAGGLLLGVLFVCVGAGALIGWAAGSVGI
                     GMAIGSVIGIPAAILTVYRVYREAF"
     gene            953..1333
                     /locus_tag="VFO88_01630"
     CDS             953..1333
                     /locus_tag="VFO88_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544257.1"
                     /translation="MLAIRPMPERRLPVAAAATVVALALPLFLVAGWRIEGWLLGAVL
                     WGGSQLLGLVFSRVGIGEPTLRGSGIVAFGMMGRGILVALAAIAVAVNDPYLAAAGAL
                     VYAAGYSTELATSLVLYFSGEARR"
     gene            1330..2154
                     /gene="atpB"
                     /locus_tag="VFO88_01635"
     CDS             1330..2154
                     /gene="atpB"
                     /locus_tag="VFO88_01635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9544258.1"
                     /translation="MSPFPLAAEEFDPAHEFELPAWVSLELGPIDMSINKAVAYLWLG
                     GLVTILFGIFFMRFGLSLRPSRRQTMGESLYELLQSQIAEGNLPKKSVGRWFPYVAAL
                     FLFIWILNMIGFIPLPLSDERFELFGLELPTWGVYAATANLSVTLALALITFFATHVE
                     GLRYNGAVGYFKSWIPAGVPKGLLILIVPLEIISQFMRLISLSVRLFANMLAGHMLIL
                     VMIGLTFIISGWALIIALPAATAIYLFEVVIVVTIQAFVFALLSAIYIGGAIEPEH"
     gene            2169..2429
                     /locus_tag="VFO88_01640"
     CDS             2169..2429
                     /locus_tag="VFO88_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET9544259.1"
                     /translation="MEFLLAQATGDVADLGKAIAFGVGVGLGAVGAGIGIGNIFGSMI
                     QAVARQPELRGELTGIQWLGFALVEAVVFYALLGGLLAFVLV"
     gene            2430..2984
                     /gene="atpF"
                     /locus_tag="VFO88_01645"
     CDS             2430..2984
                     /gene="atpF"
                     /locus_tag="VFO88_01645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET9544260.1"
                     /translation="MTGLPLILAQEEGAGDALIRPIPGLMIWTFITFLIVFLVLRKLA
                     FGRIQGLIDQRRDRIREALDEADKAREEARQLRELTQQEREEAKLERERQLEEVRRQA
                     AEQLRRSREEADQDLQRRLEENQKAIEAENRKLREEIRRDVVELTLLASEKVTRKSLD
                     ADDQKRLIDETIAEIDAEQLAREN"
     gene            2992..3525
                     /gene="atpH"
                     /locus_tag="VFO88_01650"
     CDS             2992..3525
                     /gene="atpH"
                     /locus_tag="VFO88_01650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET9544261.1"
                     /translation="MAVVHKVYAEALLGAAKDAKRVDEVRDEFAGFVEALRSSDELVD
                     LLRNPQIDPRAKQESLQAVLAGADETFLNFVRLVAEKGRAGELDAIHDEWERLLAAEE
                     RILELELTTAVELSDEEAAAVVRKIEEASGRKVEATRTVDPDLIGGLVLRAGSVRVDG
                     SVRGRLNSLRDELLTRG"
     gene            complement(3522..>4831)
                     /locus_tag="VFO88_01655"
     CDS             complement(3522..>4831)
                     /locus_tag="VFO88_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9544262.1"
                     /translation="QTRVCPAELCEHRLEPARERFRSLFSDARVVYLYLVSPPALEDR
                     LPAIDEAWADFTDDGGSGAPAEPKDRGGLPKFSAATFHLGRDEDFIRFVASVRGPEEP
                     ARPSLDFLHALLPHGPWLRWPDGRESAAKNTRAPGRIGEHWRNAALARQAYQRHLLQL
                     GYTDGLLGALLDRLHELDLWDRALVVVTADHGVSFRGGDMRRTPTATNLADLGFTPLF
                     VKLPGQRAGRVSEKHVTTADLLPTIADALGVEVPWSVDGTSGFEPGDGPDELRVGTVS
                     APFAEALEQRERALARQVDLFGTGAFDERFFALGPFAELVGEMVDALVVEAGAEGAAR
                     IEPPVSGLLAALPEDSSAVPSPITGVLTGEAVAGDALAVAVNGRIAAVCEAYRPSGGD
                     VQFSALAPATAFRPGENRVQVFLVGGAAERPVLRELPVELEGL"
BASE COUNT          660 a         1750 c         1735 g          686 t
ORIGIN      
        1 gggcggcgag atccccctcg agcggcgcga gctcacgctc gtgctccggc cgcggcagat
       61 ctcgggcttc ggcgggtgca acgagttcgg cgcgcgggcg cggatcgcgg gcgagcgcat
      121 ccgcgtgcgc ggcccgctga cctcgaccct gatgggctgc ggggccgaga tcaacgccgt
      181 cgaggaggcc tacttcgacg cgctccggga cgtcgagcgg cgggagctga aggacggacg
      241 gctcgtcctg accggccccg agacggagct cgtcttcgag ccgctcgcgc ccgtcgaccc
      301 cgagcggctc gacgggcgga cgtggacgct cgagtcctgg accgtcgacg gcgccacgcg
      361 cacggcccgc gcgcggccgg cgacgctccg cttcggccgc gacgggcgcg tcgaggcgca
      421 cacgcggtgc cgcgacctcg ccggccgcta catcctccag ggccacgagg tcgtgctcac
      481 ggaggcggag atgagccgcc gcgaccatgc cggctgctcg cgcgaggcga tcgagcagga
      541 gggagcggtc gtcggcgcgc tcggggaccg ctttcgggtc gacgtcgacg gcgaccggct
      601 ccggatcgag tcgcgcgacg gctacgtgct cgtcctgcgg gccgactgac gccgccgcac
      661 aaagttcacc ggcaagcggc cagggacgcg ctttccggct gtcacgaact cggctcccgc
      721 tgttaagctt cgccccgcgt ggaatgatgc cgaccgcaat cgccgaccgc ccgacattcg
      781 acccgctgag cgccggaggg ctcctcctcg gagtcctctt cgtctgcgtg ggcgccggcg
      841 cgctgatcgg ctgggcggcc ggctcggtcg ggatcgggat ggcgatcggc tccgtgatcg
      901 gcatccccgc cgcgatcctc accgtgtacc gcgtgtaccg ggaggccttc tagtgctcgc
      961 gatccgcccg atgccggagc ggcgcctgcc ggtcgccgcg gccgcgaccg tcgtcgccct
     1021 cgcgctgccg ctcttcctcg tcgcaggctg gcggatcgag ggctggctcc tcggcgccgt
     1081 gctctggggc ggcagccagc tcctcgggct cgtcttctcg cgcgtcggga tcggcgagcc
     1141 gacgctgcgc gggtccggga tcgtcgcctt cgggatgatg ggccgcggga tccttgtcgc
     1201 cctcgccgcg atcgcggtcg ccgtgaacga cccgtacctc gccgccgcgg gcgcgctcgt
     1261 ctacgcggcc ggctactcga ccgagctcgc cacgagcctc gtcctctact tttccgggga
     1321 ggcgcgccgg tgagcccctt cccgctcgcc gcggaggagt tcgacccggc ccacgagttc
     1381 gagctcccgg cctgggtctc gctcgagctc gggccgatcg acatgtcgat caacaaggcg
     1441 gtcgcgtacc tctggctcgg cggcctcgtg acgatcctct tcgggatctt cttcatgcgc
     1501 ttcgggctct ccctccgccc gagccggcgc cagacgatgg gcgagagcct ctacgagctc
     1561 ctccagtccc agatcgccga ggggaacctc ccgaagaagt cggtcggccg ctggttcccg
     1621 tacgtcgcgg cgctcttcct cttcatctgg atcctcaaca tgatcgggtt catcccgctc
     1681 ccgctctcgg acgagcgctt cgagctcttc gggctcgagc tgcccacctg gggggtgtac
     1741 gcggcgaccg cgaacctgag cgtgacgctc gctctcgcgc tgatcacgtt cttcgcgacg
     1801 cacgtggagg gcctgcgcta caacggcgcc gtgggctact tcaagagctg gatcccggcc
     1861 ggcgtgccga agggcctgct catcctcatc gtcccgctcg agatcatctc gcagttcatg
     1921 cgcctgatct cgctctccgt gcgtctcttc gcgaacatgc tcgcggggca catgctcatc
     1981 ctcgtgatga tcggcctcac gttcatcatc agcggctggg cgctcatcat cgcgctcccg
     2041 gccgcgaccg cgatctacct cttcgaggtg gtgatcgtcg tcaccatcca ggccttcgtc
     2101 ttcgccctgc tctccgcgat ctacatcggc ggagcgatcg aacccgaaca ctaggaaagg
     2161 gaggaaccgt ggaatttctg cttgcacagg cgacggggga cgtcgccgac ctcgggaagg
     2221 cgatcgcctt cggcgtcggc gtcggcctcg gcgcggtagg cgccggcatc ggcatcggca
     2281 acatcttcgg ctcgatgatc caggcggtcg cgcgccagcc ggagctgcgc ggcgagctga
     2341 ccggcatcca gtggctcggg ttcgcgctcg tcgaggccgt cgtcttctac gcgctcctcg
     2401 gcgggctcct cgccttcgtc ctcgtctaga tgaccgggct cccactcatc ctggcgcagg
     2461 aggagggcgc gggcgacgcc ctgatccggc ccatcccggg cctcatgatc tggaccttca
     2521 tcacgttcct gatcgtcttc ctcgtcctgc gcaagctcgc gttcgggcgg atccaggggc
     2581 tgatcgacca gcgccgcgac cggatccgcg aggcgctcga cgaggcggac aaggcgcgcg
     2641 aggaggcccg ccagctccgc gagctgaccc agcaggagcg cgaggaggcg aagctcgagc
     2701 gcgaacggca gctcgaggag gtccgccgcc aggcggccga gcagctccgc cgctcgcgcg
     2761 aggaggccga ccaggatctc cagcgccggc tcgaggagaa ccagaaggcg atcgaggccg
     2821 agaacagaaa gctccgcgag gagatccgcc gcgacgtcgt cgagctcacc ctcctcgcct
     2881 cggagaaggt gacgcggaag tcgctcgacg cggacgacca gaagcggctg atcgacgaga
     2941 cgatcgccga gatcgacgcc gaacagctgg cgcgcgagaa ctaggggcgc cgtggccgtc
     3001 gtccacaagg tgtacgcgga ggcgctgctc ggcgccgcga aggacgcgaa gcgcgtggac
     3061 gaagtgcgcg acgagttcgc cgggttcgtc gaggcgctgc gctcctccga cgagctcgtc
     3121 gacctgctcc gcaacccgca gatcgacccg cgcgcgaagc aggagtcgct ccaggccgtg
     3181 ctcgccgggg cggacgagac gttcctgaac ttcgtccgcc tcgtcgccga gaagggccgc
     3241 gccggcgagc tcgacgcgat ccacgacgag tgggagcgcc tgctcgccgc cgaggagcgg
     3301 atcctcgagc tcgagctgac gacggccgtc gagctctcgg acgaggaggc ggccgcggtc
     3361 gtgcggaaga tcgaggaggc ctccgggcgg aaggtcgagg cgacgcggac ggtcgacccc
     3421 gacctgatcg gcggcctcgt cctgcgcgcc ggctccgtgc gggtcgacgg cagcgtgcgc
     3481 gggcggctga acagcctccg cgacgagctc ctgacgcgcg gttagaggcc ttcgagctcg
     3541 accgggagct cgcggaggac gggacgctcg gcggcgccgc ccacgaggaa gacctgcacg
     3601 cggttctccc cgggccggaa cgccgtcgcc ggcgcgagcg ccgagaactg gacgtcgccg
     3661 ccggacgggc ggtaggcctc gcagacggcg gcgatcctgc cgttcacggc gacggcgagc
     3721 gcgtccccgg ctaccgcctc gccggtcaga acgcccgtga tcggcgacgg cacggcggag
     3781 gagtcctccg gaagcgcggc caggagcccg ctgaccggcg gctcgatccg tgccgcgccc
     3841 tcggcgccgg cctcgacgac cagggcgtcg accatctcgc cgacgagctc cgcgaacggc
     3901 ccgagggcga agaagcgctc gtcgaacgcg cccgtcccga acaggtcaac ctgccgggcg
     3961 agcgcacgct ctcgctgctc gagcgcctcc gcgaacggcg ccgagaccgt gcccacacgg
     4021 agctcgtccg ggccgtcgcc gggctcgaac cccgacgtcc cgtccaccga ccagggcacc
     4081 tcgacgccga gcgcgtcggc gatcgtgggg aggaggtccg ccgtcgtcac gtgcttctcc
     4141 gacacgcgcc ccgcccgctg tcccgggagc ttgacgaaga gcggcgtgaa gccgaggtcg
     4201 gcgaggttcg tcgcggtcgg ggtgcggcgc atgtcgccgc cgcggaagct cacgccgtgg
     4261 tcggccgtga cgacgacgag cgcccggtcc cagaggtcga gctcgtggag ccggtcgagg
     4321 agcgcgccga gcagcccgtc cgtgtacccg agctggagga ggtgccgctg atacgcctgc
     4381 cgcgccagcg ccgcgttgcg ccagtgctcg ccgatgcggc cgggcgcgcg ggtgttcttc
     4441 gcggcgctct cgcggccgtc cggccagcga agccaggggc cgtgcgggag gagcgcgtgg
     4501 aggaagtcga gcgagggacg ggcgggctcc tccggccccc gcaccgaggc gacgaaccgg
     4561 atgaagtcct cgtcccggcc gagatggaag gtcgccgcgg agaacttcgg cagccccccg
     4621 cgatccttcg gctcggccgg cgcgccgctc cccccgtcgt cggtgaagtc cgcccacgcc
     4681 tcgtcgatcg cgggcagccg gtcctcgagc gcgggcgggg agaccaggta gaggtagacg
     4741 acccgtgcgt ccgagaagag cgaccggaac cgctcccgcg ccggctcgag acggtgctcg
     4801 cagagctccg cgggacacac gcgcgtctgc g
//
LOCUS       DASTFC010000057         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1006411,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000057 DASTFC010000000
VERSION     DASTFC010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006411"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFO88_01660"
     CDS             <1..354
                     /locus_tag="VFO88_01660"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544263.1"
                     /translation="VHQLTREDLVQILLEPKNALVKQYQRFFNYDSIELIFTEDALWE
                     ISDKALKRETGARGLRSIIEHALLDVMFELPSRKDVNKCVITKETIERNLRPTLVTTG
                     GRDADDDEALAEETA"
     gene            complement(351..554)
                     /locus_tag="VFO88_01665"
     CDS             complement(351..554)
                     /locus_tag="VFO88_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544264.1"
                     /translation="MDVKTIGEQGLHGWKGSVAHSVARRADRSRIPVTETQVRAVLGV
                     AFFTMSLLYVVKTLRAVAHEARD"
     gene            complement(590..>2135)
                     /locus_tag="VFO88_01670"
     CDS             complement(590..>2135)
                     /locus_tag="VFO88_01670"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9544265.1"
                     /translation="EALRAHNAHDLDLAIELGRRAEELGRRLGDRGLEVRGLQRRGAA
                     LIAKGEVATGRALLDEAATAAVGGELDPYSTVVVYCNTIGACRDVADFDRAGEWTDLA
                     HAFCTSSSMSAFPGMCRVNYAEVMKFKGRLDQASEEAIRAGEELRGWLPRVAAAAFYE
                     LGEIRLRLGDLAEAERSFREADEFGRHPEPGLSLLRLAKGNAKAAWSSIRRAIADDGL
                     TAPVRVRLLPAAIEIALAAGEQDAAAPLVEELAEAAERFETSAVKAQAAYAKGALALA
                     DGDPGAAFGALREARRLWDATGATYDVARARELLGLAARADGDEESAVWELRAAAARF
                     ERLGALRDADRVAAHLARDGARQVTKTFLFTDIVRSTELLETIEDRHWANVLRRHDDT
                     LRAIFSDHGGEVVDHTGDGFFVAFEDAAEAAHAAMAVQRAVDQEFVFDVRIGLHMDGA
                     LARGENYHGRGVHTAARVGAAAEGREILATVETVEGLDVPVSEPRPLTLKGIKAPIET
                     VAIHWS"
BASE COUNT          293 a          800 c          765 g          277 t
ORIGIN      
        1 gtgcaccagc tgacgcgcga ggacctcgtg cagatcctcc tcgagcccaa gaacgcgctc
       61 gtcaagcagt accagcgctt cttcaactac gactcgatcg agctgatctt caccgaggac
      121 gccctctggg agatctccga caaggcgctc aagcgcgaga ccggcgcccg cggcctgcgc
      181 tcgatcatcg agcacgcgct cctcgacgtc atgttcgagc tgccgtcccg caaggacgtg
      241 aacaagtgcg tgatcacgaa ggagacgatc gagcggaacc tccgcccgac cctcgtgacc
      301 acgggcggcc gcgacgcgga cgacgacgag gcgctcgccg aagaaaccgc ctagtcgcgg
      361 gcttcgtgcg cgacggcgcg gagggtcttc acgacgtaga gcagcgacat cgtgaagaag
      421 gcgacgccga ggacggcgcg cacctgcgtc tcggtcacgg ggatccgcga gcggtccgcg
      481 cggcgggcga ccgaatgcgc gacgctcccc ttccagccgt gcaggccctg ctcgccgatc
      541 gtcttcacgt ccatgggtgg cgggataccc gcacgggccg cggccgaacc tagctccagt
      601 ggatcgcgac cgtctcgatc ggcgccttga tgcccttgag cgtgagcggg cgcggctccg
      661 agacggggac gtcgagccct tcgaccgtct cgacggtcgc gaggatctcg cgcccctccg
      721 cggcggcccc gacacgtgcc gcggtgtgga cgccgcggcc gtggtagttc tccccgcgcg
      781 cgagggcgcc gtccatgtgc aggccgatcc gcacgtcgaa gacgaactcc tggtcgaccg
      841 cgcgctgcac cgccatcgcc gcatgggcgg cctcggcggc gtcctcgaac gcgacgaaga
      901 agccgtcgcc ggtgtggtcc accacctcgc cgccgtggtc ggagaagatc gcgcggagcg
      961 tgtcgtcgtg gcggcgcagg acgttcgccc agtgccggtc ctcgatcgtc tccaggagct
     1021 ccgtcgagcg gacgatgtcc gtgaagagga acgtcttcgt cacctgccgc gcgccgtcgc
     1081 gcgcgaggtg cgcggcgacc cggtcggcgt cgcggagggc gcccaggcgc tcgaagcggg
     1141 cggccgccgc gcggagctcc cacacggcgg actcctcgtc cccgtcggcg cgcgcggcga
     1201 gcccgagcag ctcgcgggcg cgcgcgacgt cgtacgtcgc gccggtcgcg tcccagagcc
     1261 ggcgggcctc gcggagcgcg ccgaaggccg cgcccggatc cccgtccgcg agcgcgagcg
     1321 cgcccttcgc gtacgccgcc tgcgccttga ccgccgacgt ctcgaagcgc tcggcggcct
     1381 ccgcgagctc ctcgacgaga ggcgccgcgg cgtcctgctc gcctgcggcg agcgcgatct
     1441 cgatcgccgc cggcaggagc cgcacgcgga cgggcgcggt cagcccgtcg tcggcgatcg
     1501 cgcgccggat cgacgaccag gccgccttcg cgttcccctt cgcgagccgc aggagcgaga
     1561 gaccgggctc cgggtggcgg ccgaactcgt ccgcctcgcg gaaagagcgc tcggcctcgg
     1621 caaggtcgcc gagccgcaag cggatctcgc cgagctcgta gaacgccgcg gcggcgacgc
     1681 gggggagcca gccgcgcagc tcctcgccgg cgcggatcgc ctcctcgctc gcctggtcga
     1741 gccggccctt gaacttcatg acctccgcgt agttcacgcg gcacatgccg ggaaacgcgc
     1801 tcatggagct cgacgtgcag aaggcgtggg cgaggtcggt ccactcgccc gcccggtcga
     1861 agtcggcgac gtcccggcac gcgccgatcg tgttgcagta gacgacgacc gtcgagtacg
     1921 ggtcgagctc gccgccgacc gccgccgtcg ccgcctcgtc gaggagcgcg cggccggtcg
     1981 cgacctcgcc cttcgcgatg agcgcggcgc ccctgcgctg gaggccgcgc acctcgagcc
     2041 cgcggtcgcc gagccgccgg ccgagctcct cggcgcggcg accgagctcg atcgcgaggt
     2101 cgaggtcgtg cgcgttgtgc gcgcgcagcg cttcc
//
LOCUS       DASTFC010000058         4632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1204124,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000058 DASTFC010000000
VERSION     DASTFC010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204124"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFO88_01675"
     CDS             complement(<1..272)
                     /locus_tag="VFO88_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9544266.1"
                     /translation="MASRANFRPPQQRRSAATLDRIVQAAEELLAERTFDEATVDDIV
                     ARAGSSKGSFYSRFADKESLLAYLGGECIARSKATWAEQLGPEDYA"
     gene            complement(296..1291)
                     /locus_tag="VFO88_01680"
     CDS             complement(296..1291)
                     /locus_tag="VFO88_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9544267.1"
                     /translation="MRQVVARRAGGPEVLEVVDVPEPAPGPGEVVVRTEAIGVNFADV
                     WIRMGADGEPPFTPGIEVAGHVVAVGEEVESRHVGERVVATPHETRCGYAELVRAPAE
                     LAFPLPDDLEFTLAAALPLNYLTAYAAVEHAARPRWRERVLVHAAAGGVGLAAVQLAR
                     RREAELYATASAGKHDALLEEGVAHAIDYRTDDWVEEIHRLTGDGVDVVVDGVGEDGF
                     RKSLGVLRFGGRVVAYGFTSGVAGPTAELPDLEDELATGVPLLELLSDARGLVGVHMG
                     APAPILHRWWADILAWLERDEIAPRIDRVLPLERAAEAHARLHARENVGKIVLVP"
     gene            complement(1322..2461)
                     /locus_tag="VFO88_01685"
     CDS             complement(1322..2461)
                     /locus_tag="VFO88_01685"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HET9544268.1"
                     /translation="MRITRRELAGAVADLGVLVPIAVALIVSNGLSPTAVLLPAGLLY
                     LANAAIYGLPLPSQPLKAFGAIAIAEGLGADEIAAGALLIGALFLVAGRLGLVDRLAR
                     AFPRPLVRGVQLAVGLLFLQIAWNLAADPPAAFAEHALPPVWAVGLALAAAAVALAFR
                     GQGAALALVAVGAAAMVATAGGSASLGPSALAFPSLDLGVLATAFVVLVVPQAPLSFA
                     NSCLATADAARVYFGEERARRVRPGSLASTYGVANLFAGSIGGMPVCHGAGGLTAHYT
                     FGARTAAAPLVMGSVLVVLALAVGSGLAALLVAFPLPILAALLGVAGLLHIGLLRDLQ
                     GAPAWAAALLVGAVGATVGLAVGLAAGLALWWLPVAVRRARLVLD"
     gene            2480..3859
                     /locus_tag="VFO88_01690"
     CDS             2480..3859
                     /locus_tag="VFO88_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9544269.1"
                     /translation="MFFRQVLHEDLGCASYVVADGGEAAIIDPKWEIEEYLELADEHG
                     FEIRHVLETHTHADHVSGHGRLRAATGATIHVSIDAGAEYEHEPLSDGDTVEVGDARI
                     TALATPGHRPEHLAFLVADGSRGEEPWLVVTGDSLFVGDLARPDLAVEAEEGAHGLYR
                     SLRRLLELDDFAEVWPGHIGGSLCGGAGMSEKPGSTIGFERRYNRFASLADEREFVRT
                     LTGKLAPQPPNFKRIVELNRGPLLTESARVDPLSPIRVAELLDAGATLLDGRDTRDFD
                     AAHVPGSINVTMVRAAVGTRAAWVVEPQADVVVTAASDEEARRMARLLEAVGFRSIRG
                     LLSGGVAAWRDAGHPTESTPAIDIESLADRLRAGDVVLLDVREEDEWEEGHVEGSLHV
                     PYHDLRDGLPELPANGKTLAVACSAGNRSSIAVSLLRRAGVNDLIHVTDGGIADLEQE
                     GIELVRYPA"
     gene            3893..3965
                     /locus_tag="VFO88_01695"
     tRNA            3893..3965
                     /locus_tag="VFO88_01695"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3926..3928,aa:Met,seq:cat)
     gene            4017..4166
                     /gene="rpmG"
                     /locus_tag="VFO88_01700"
     CDS             4017..4166
                     /gene="rpmG"
                     /locus_tag="VFO88_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET9544270.1"
                     /translation="MRVGITLACTECKRRNYQTNKSKRNTPDRFEAKKYCRWCGGHTL
                     HRETR"
     gene            4171..4497
                     /gene="secE"
                     /locus_tag="VFO88_01705"
     CDS             4171..4497
                     /gene="secE"
                     /locus_tag="VFO88_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET9544271.1"
                     /translation="MARPSRARRGNYQAPATPARRRRDRQPQHKPAQQTKSQAGAQRE
                     RRGGVRTFVGESAAELRKVDWPGQRQLFMGTVVVLIAIAVVGAYLAAADFVFSRFVQD
                     VLLRGI"
     gene            4500..>4632
                     /locus_tag="VFO88_01710"
     CDS             4500..>4632
                     /locus_tag="VFO88_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014469615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HET9544272.1"
                     /translation="MFRWYVINTYSGHENKVKANLEHRIDSMKQAERFRRVVVPTEQR
                     "
BASE COUNT          689 a         1701 c         1703 g          539 t
ORIGIN      
        1 tccgcgtagt cctcgggccc gagctgctcc gcccaggtcg ccttcgagcg cgcgatgcac
       61 tcgccgccga ggtaggcgag cagggattcc ttgtccgcga agcgcgagta gaacgacccc
      121 ttggaggagc cggcgcgcgc gacgatgtcg tccacggtcg cctcgtcgaa ggtgcgctcg
      181 gcgagcagct cctcggccgc ctgcacgatg cggtcgaggg ttgccgcact ccgccgctgc
      241 tgcggcggac ggaagttcgc ccgggacgcc atccccggct cattgtgact gttggctacg
      301 gcacgagcac gatcttcccc acgttctcgc gcgcgtggag gcgtgcgtgg gcctccgcgg
      361 cgcgctcgag cgggaggacg cggtcgatcc gcggggcgat ctcgtcgcgc tcgagccagg
      421 cgaggatgtc cgcccaccag cggtggagga tcggcgccgg cgcgcccatg tgcacgccga
      481 cgagcccgcg cgcgtccgag aggagctcga gcagcgggac gccggtcgcg agctcgtcct
      541 cgagatccgg cagctcggcc gtcggcccgg cgacgccgga cgtgaagccg taggcgacga
      601 cgcggccgcc gaagcggagg acgccgaggc tcttccggaa cccgtcctcg ccgacgccgt
      661 ccacgacgac gtcgacgccg tccccggtca ggcggtggat ctcctcgacc cagtcgtcgg
      721 tgcggtagtc gatcgcgtgc gcgacgcctt cctcgaggag cgcgtcgtgc ttccccgccg
      781 acgcggtcgc gtagagctcc gcctcgcgcc ggcgcgcgag ctggactgcg gcgaggccga
      841 cgccgcccgc ggccgcgtgc acgagaacgc gctcgcgcca ccgcggccgc gccgcgtgct
      901 cgacggccgc gtacgccgtc aggtagttga gcgggagggc ggccgcgagc gtgaactcga
      961 ggtcgtcagg gagggggaag gcgagctcgg ccggggcgcg cacgagctcg gcgtagccgc
     1021 agcgcgtctc gtgcggcgtc gcgaccaccc gctcgccgac gtggcgcgac tcgacctcct
     1081 cgccgaccgc gaccacgtgt cccgccacct cgatcccggg cgtgaacgga ggctccccgt
     1141 ccgcgcccat ccggatccac acgtccgcga agttgactcc gatcgcctcg gtgcggacga
     1201 cgacctcgcc ggggcccgga gcgggctcgg gcacgtccac gacctcgagg acctctggcc
     1261 cgccggcgcg gcgggcgaca acctggcgca tgggctcgcc tccagggaag gactagaccg
     1321 atcagtctaa tactagccga gcgcgccgga ccgcgacggg aagccaccag agggcgaggc
     1381 cggcggcgag gccgaccgcg agcccgaccg tcgcgccgac cgctcccacg agcagcgccg
     1441 ccgcccaggc cggcgcgccc tgcagatcgc gtagcagccc gatgtggagg agcccggcca
     1501 cgccgaggag cgccgcgagg atcgggagcg gaaacgcgac gagtagcgcc gcgagccccg
     1561 acccgacggc gagcgcgagg acgacgagga cgcttcccat gacgagcggt gcggcggccg
     1621 tgcgcgcgcc gaacgtgtag tgggcggtga gtccgcccgc tccgtggcag accggcatcc
     1681 cgccgatcga gccggcgaag aggtttgcca ccccgtacgt ggacgcgagg gagcccggcc
     1741 gcacgcgccg cgcgcgctcc tccccgaagt acacgcgtgc ggcgtccgcc gtcgcgaggc
     1801 aggagttcgc gaacgagagc ggcgcctgcg ggacgacgag cacgacgaac gcggtcgcga
     1861 ggacgccgag gtcgagcgag gggaaggcca gcgcggacgg cccgagcgag gccgagccgc
     1921 ccgccgtcgc caccatcgcc gccgcgccga cggcgacgag cgcgagcgcc gcgccctggc
     1981 cgcggaacgc gagcgcgacc gccgccgcgg cgagcgcgag ccccacggcc cagaccggcg
     2041 gcagcgcgtg ctcggcgaac gcggccggcg ggtcggccgc gaggttccac gcgatctgga
     2101 ggaagaggag gccgaccgcg agctgcaccc cgcgcacgag cggccgcggg aacgcgcgcg
     2161 ccagccggtc gacgaggccg agccgcccgg cgacgaggaa gagcgccccg atgaggagcg
     2221 ccccggccgc gatctcgtcc gcgccgaggc cctccgcgat cgcgatcgct ccgaacgcct
     2281 tcagcggctg cgaggggagc ggcagcccgt agatcgccgc gttcgcgagg tacaggaggc
     2341 ccgcgggcag gagcaccgcg gtcggcgaga gcccgttcga gacgatgagc gcgacggcga
     2401 tcggaacgag gacgccgagg tcggcgaccg ccccggccag ctcccggcgg gtgatgcgca
     2461 cgcgcatatc atggcgcgcg tgttcttccg gcaggtgctg cacgaggatc tcgggtgcgc
     2521 gtcgtacgtc gtggccgacg gcggcgaggc ggcgatcatc gacccgaagt gggagatcga
     2581 ggagtacctc gagctcgccg acgagcacgg cttcgagatc cgccatgtgc tcgagacgca
     2641 cacgcacgcc gaccacgtct cgggccacgg gcggctgcgg gcggcgacgg gcgccacgat
     2701 ccacgtctcc atcgacgcgg gcgcggagta cgagcacgag ccgctctcgg acggcgacac
     2761 ggtcgaggtc ggcgacgcgc ggatcaccgc gctcgcgacg ccgggccacc ggcccgagca
     2821 tctcgcattc ctcgtcgcgg acgggagccg cggcgaggag ccgtggctcg tcgtgaccgg
     2881 cgactcgctc ttcgtcggcg acctcgcccg gcccgacctc gcggtcgagg cggaggaggg
     2941 cgcgcacggc ctctaccgct cgctccgccg gctgctcgag ctggacgact tcgccgaggt
     3001 ctggcccggc cacatcggcg gctcgctctg cggcggggcg gggatgagcg agaagcccgg
     3061 ctcaacgatc ggcttcgagc gccgctacaa ccgcttcgcg tcgctcgccg acgagcgcga
     3121 gttcgtgcgc acgctgaccg gcaagctcgc gccgcagccg ccgaacttca agcggatcgt
     3181 cgagctgaac cgcgggccgc tcctcaccga gtccgcgcgc gtcgacccgc tctccccgat
     3241 ccgcgtcgcc gagctcctcg acgcgggcgc gacgctcctc gacggccgcg acacgcgcga
     3301 tttcgacgcc gcgcacgtgc cgggctcgat caacgtgacg atggtgcgcg ccgcggtcgg
     3361 aacgcgcgcc gcgtgggtgg tcgagcccca ggcggacgtc gtggtgactg cggcgtcgga
     3421 cgaggaggcg cgccgcatgg cgcggctgct cgaggcggtc ggcttccgct cgatccgcgg
     3481 cctgctctcc ggcggggtgg ccgcgtggcg cgacgccggc catccgaccg agtcgacgcc
     3541 cgcgatcgac atcgagagcc tcgccgaccg cctgcgcgcc ggcgacgtcg tcctgctcga
     3601 cgtgcgcgag gaggacgaat gggaggaggg gcacgtcgag ggctcgctcc acgtccccta
     3661 ccacgacctc cgcgacggcc tgcccgagct tcctgcgaac ggcaagacgc tggccgtcgc
     3721 ctgctcggcg gggaaccgca gctcgatcgc cgtctcgctc ctccggcgcg ccggtgtgaa
     3781 cgacctgatc cacgtgaccg acggcgggat cgccgacctg gagcaggaag gaatcgagct
     3841 cgtccgctac cctgcctgag tccgctctgc tgctagactt gcgagccgcc tcggcggcgt
     3901 agctcagtgg ttagagcggc ggactcataa cccgcaggtc ggtggttcga atccacccgc
     3961 cgctactccg cccacgcaat ccctaggacg aagagagaag aacgttggca actggagtga
     4021 gagtaggaat cacgctcgcc tgcaccgagt gcaagcggcg taactaccag acgaacaagt
     4081 cgaagcggaa cacgcccgac cggttcgagg cgaagaagta ctgccgctgg tgcggcggcc
     4141 acacgctcca ccgggaaacg cggtagagcg atggcccgtc cctcccgcgc acgccgtggc
     4201 aactaccagg cgcccgcgac cccggcgcgg cgccgtcgcg accgccagcc gcagcacaag
     4261 cccgcccagc agacgaagag ccaggccggg gcgcagcgcg agcgccgcgg cggcgtccgc
     4321 accttcgtcg gcgagtcggc ggccgagctc cgcaaggtcg actggccggg ccagcgccag
     4381 ctcttcatgg gcaccgtcgt cgtgctgatc gcgatcgccg tcgtcggcgc gtacctcgcg
     4441 gccgcggact tcgtcttcag ccgcttcgtc caggacgtcc tcctgagagg aatttgagcg
     4501 tgttccgctg gtacgtgatc aacacctact cgggccacga gaacaaggtc aaggcgaacc
     4561 tcgagcaccg catcgactcg atgaagcagg cggagcggtt ccgccgcgtc gtcgtgccca
     4621 ccgagcagcg ga
//
LOCUS       DASTFC010000059         6829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_297790,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000059 DASTFC010000000
VERSION     DASTFC010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297790"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFO88_01715"
     CDS             complement(<1..381)
                     /locus_tag="VFO88_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544273.1"
                     /translation="MPTSAPAIAPMLRAFLRALDEAGIRWTLLRPVASLAAEEGDVDV
                     LVEPASLAAVEAHAERLGYVLVPFAPPDVHATTYDEEAGRFVWVHVQGELRLAGAVFP
                     AEDVLGEAVREDDAARPGDGWLLWI"
     gene            complement(431..715)
                     /locus_tag="VFO88_01720"
     CDS             complement(431..715)
                     /locus_tag="VFO88_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544274.1"
                     /translation="METWLWILVALPFVAGGALFVWELISLDRGRAGTESRPADPGTD
                     DLAAAVRHRARKHAEPGRSSADDYAWEDRPPPQRRNVEREHAPRNDAAGP"
     gene            complement(1182..1254)
                     /locus_tag="VFO88_01725"
     tRNA            complement(1182..1254)
                     /locus_tag="VFO88_01725"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1219..1221),aa:Ile,seq:cat)
     gene            1372..1545
                     /locus_tag="VFO88_01730"
     CDS             1372..1545
                     /locus_tag="VFO88_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544275.1"
                     /translation="MIRCGECRCVADDADEGWLTYLIAEDREEPDFIPYFLSYCPSCS
                     FREFGVIPRTAYT"
     gene            1675..2094
                     /locus_tag="VFO88_01735"
     CDS             1675..2094
                     /locus_tag="VFO88_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9544276.1"
                     /translation="MVAILTGCGGGDGGGGTGTSEAGAAPSASLSVAASEFAFEPAEL
                     TADAGEVTVELVNDGAAPHAIAIAGNGVDEASETVDGGATTSLTVELEDGTYDIWCPV
                     GDHRDRGMVGTLTVGAGGGASDGGTDTGETSDDSPRY"
     gene            2190..2678
                     /locus_tag="VFO88_01740"
     CDS             2190..2678
                     /locus_tag="VFO88_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET9544277.1"
                     /translation="MKVFQHETSLRTKGGLEITDITDEVREAVTKSGVRDGIACVYSP
                     HTTCCVRVNEFETGFLEDFVQMLRRLVPAEEYYAHDDWDRRTENICPEDMEFGNGHSH
                     CMAMLLGPAGESIPVRDGELCLGTWQRVLFMELDRSRDRRWIVQVVGDGPPASASNGA
                     SA"
     gene            complement(2908..4791)
                     /locus_tag="VFO88_01745"
     CDS             complement(2908..4791)
                     /locus_tag="VFO88_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HET9544278.1"
                     /translation="MDSERIFAGLNPEQRRAVEAVRGPVCILAGAGSGKTTTITRRIA
                     NQVATGAFRAESVLAVTFTDKAAGEMRSRLEALGATGVRARTFHAAALGQLHALGAAP
                     PGGILPSKAIVLTNVARGLPKPYRFRPVGDLATEIEWAKNRRLTPATYTEAAMGRRAP
                     LPPDLMVGVFQGYERRKEALGKVDFEDVLELAIRLFAEDEVARERFHRTIGAITVDEY
                     QDVNLLQQTLLDLWLGGRDELCVVGDDHQAIYSFTGATPAHLLGMPERYPHATVVRLE
                     ENYRSTPQVLELANRLAPRLGGAEKALRAVLPDGPEPTAEALDGPEETRFVVERVREL
                     AAEGVPLGEIAVLYRTNARSAGFEQAFTEAGIPYQVREGGFLLRQAARRLRARLRNAS
                     GSTAVAASVRAAAGEEGWVPAPEKELGEQELVRQADFAQFVDLAERLDDGVRTIAGFW
                     AELEERFGPDSVADRGVQLLTYHRAKGLEFEAVFLPRLEEKELPIRQAKSDGEIDEER
                     RLLYVGITRAKRRLYLTRSVDAKPSRFLVELGLADERATARREKTPLEDTPEVRALRK
                     WRLERAKADGVPAYVVFHDSTLAAIAAQRPSSRHELADVPGIGPTKLERYGDDVIAAL
                     QAA"
     gene            complement(4919..6091)
                     /locus_tag="VFO88_01750"
     CDS             complement(4919..6091)
                     /locus_tag="VFO88_01750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET9544279.1"
                     /translation="MRAVCWTGRNSVEVENVPDPQILNDRDAIVRVTSTAICGSDLHL
                     YDGYVPTMKKGDILGHEFMGEVVEVGRGVSNLGVGDRVVVPFPIACGNCNACRAEWWS
                     LCENSNPNAGLAEKLWGHSPCGIFGYSHLTGGYAGGQAEYVRVPFADVGPLKLENGIP
                     DEQVLFLSDILPTGYMGAELCDITQGDVVAVWGAGPVGQFAIASAFMLGAERVIAIDR
                     FPERLRMAAEVSGAETLNYEQEDVPEALKELTGGRGPDAAIDAVGMEAHHASLPLHAY
                     DRVKQATRLESDRAHALREAIMSVKNGGIVSVVGVYGGLVDKFPMGAVVNRSLTIKAG
                     QCHVHRYLRPLLERIENGEIDPSFVVTHRMALDDAPEGYELFKRKQDDCVKVVLSP"
     gene            complement(6088..6705)
                     /locus_tag="VFO88_01755"
     CDS             complement(6088..6705)
                     /locus_tag="VFO88_01755"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9544280.1"
                     /translation="MGTLRRAGWFGLGLGLAYAAGRGLRRETKEEPMQVRAAVTVVED
                     TGTAYALWRDVTSFPRFMAHVESVEEREDGTTHWRVASPTGTVEWDAETVEDVPGERI
                     AWRSLPGGDVETSGTVRFSPAPGGRGTEIQLELRYEAPAGSVGTLLAKLMGREPTQQA
                     KDDLRRFKQILETGEIARSDAVPGGIDASRTLRQRPARPLEEAVR"
BASE COUNT          923 a         2531 c         2417 g          958 t
ORIGIN      
        1 tatccagagg agccagccgt cccccgggcg cgcggcgtcg tcttcgcgca cggcctcccc
       61 gaggacgtcc tccgccggga acaccgcccc cgccaggcgg agctcgccct ggacgtgcac
      121 ccagacgaag cggccggcct cctcgtcgta cgtcgtcgcg tggacgtccg gcggggcgaa
      181 cgggacgagc acgtacccga gccgctcggc gtgcgcctcg acggcggcga gcgaggccgg
      241 ctcgacgagg acgtccacgt cgccctcctc ggcggcgagg ctcgcgaccg gccgcaggag
      301 cgtccagcgg atccccgcct cgtcgagcgc gcggaggaac gcccggagca tgggggcgat
      361 ggccggggcg ctcgtcggca tgcgccgacg ttatcccgga cgccggtcgg gagggacggg
      421 ctcgggtcgg ctacgggccc gccgcgtcgt tgcggggtgc atgttcccgc tcgacgttcc
      481 ggcgctgcgg aggcgggcgg tcctcccatg catagtcgtc cgccgaggaa cgccccggct
      541 cggcgtgctt cctcgcccga tgccgaaccg ccgcggcgag atcgtcggtg cccggatcgg
      601 ccggccggct ctcggtcccg gcccggcccc ggtcgaggga gatcagctcc cagacgaaga
      661 gcgcgccgcc ggcgacgaac ggcagtgcga cgaggatcca gagccatgtc tccatcggcg
      721 ccgacgctac cgacgggccg gaaagcgtcg ggcaaggctt tcgtcatgac ccggccaagg
      781 tcgcgtgcag ctgcggtagc ggcggccgcc ccgaatgccc ctctccccga gaacggagag
      841 aggggcgcca cgccgccgga tctcaaacac ttgcggagtg agagagaggg gaggccgcag
      901 cgcccctcac gcgcagagta cgcgttttcg cacggatcgg ggccgcctgg gatcggacga
      961 gcgaccggca ggccgcgaga atcccgcggc tgccctcggc ctgcaccggc cgcgttgatt
     1021 tcgctcaaca gaagcggttt tcgccggtcg cttcctgcgg cagtcgcgga gcggcggcta
     1081 cgctagccgt gtgggcgccg tggcgcgccc ctcccctggt tagccgcaga ctgggaccgg
     1141 acccccgcgc ccggcgccca ccatcctgcg catgctcttc atgggcccgc ctggattcga
     1201 accagggacc gacggattat gagtccgctg ctctgaccgc tgagctacgg gcccgcgagg
     1261 cgtctggtca acactagccc gtgcccgcga cggcttcgag cgcgtcggcg ggagcgtcgt
     1321 gcccgaaggc tgtcgctgcc cccgacccgc cgcggagact agggtgcgcg gatgatccgc
     1381 tgcggggaat gccggtgcgt tgccgacgac gccgacgagg gctggctcac ctacctgatc
     1441 gccgaggaca gggaggagcc ggacttcatc ccgtacttcc tctcctactg cccctcctgc
     1501 tcgttccgcg agttcggcgt catccctcgg acggcgtaca catgaaggtg gggctgagac
     1561 ggcgaccggg aagtcgccgc agcgccacgc cccgcaacgg ctcccggaaa ccgtctcgga
     1621 ggatccctcg tattccggac gatgcatcgt gccgccccgt acctggctct cctggtggta
     1681 gccatcctga cggggtgcgg aggcggcgat ggagggggcg gaaccggcac gtcggaagcc
     1741 ggcgccgccc cctccgcatc gctctccgtc gcggcctcgg agttcgcgtt cgagcccgcc
     1801 gagctcacgg ccgatgcggg cgaggtgacg gtcgagctcg tgaacgacgg cgcggcgccg
     1861 cacgcgatcg cgatcgcggg caacggcgtg gacgaggcct ccgagacggt cgacgggggc
     1921 gcgacgacct cgctcaccgt cgagctcgag gacgggacgt acgacatctg gtgccccgtc
     1981 ggcgaccacc gcgatcgcgg aatggtcggc acgctcacgg tcggtgccgg cggcggggcg
     2041 tcggacggcg ggaccgacac gggcgagacc tcggacgaca gcccgcgcta ctagcccccg
     2101 tccgcaagtt cgacacgaag cgcgcggaat cgccaaaata ggcgtcggac cggagtgcac
     2161 ccccgcgttt tggaggtcga gccgcagccg tgaaggtctt ccagcacgag acaagcctgc
     2221 gtacgaaggg cgggctcgag atcaccgaca tcaccgacga ggtccgggag gccgtcacga
     2281 agagcggcgt ccgcgacggc atcgcctgcg tctactcgcc gcacacgacc tgctgcgtgc
     2341 gcgtgaacga gttcgagacc gggttcctcg aggacttcgt gcagatgctc cggcgactcg
     2401 tgccggccga ggagtactac gcgcacgacg actgggaccg ccgcacggag aacatctgcc
     2461 cggaggacat ggagttcggg aacgggcact cgcactgcat ggcgatgctg ctcgggccgg
     2521 cgggcgagtc gattcccgtc cgggacggcg agctctgcct cggcacgtgg cagcgcgtcc
     2581 tcttcatgga gctcgaccgc tcgcgcgacc ggcgctggat cgtgcaggtc gtgggcgacg
     2641 gcccgcccgc gtccgcgtcg aacggcgcga gcgcctagcg gcccgggcgg cccggagcct
     2701 cgtgtcgagg cgagcccctt aaagggaaac ggggccgccg caggccccgt ctcatgaagt
     2761 ccgatccgct gcccccgccg catcggggcc tcggtcggtt atgtaggcgt actgtggccg
     2821 accgcccggc ccctgtcccc cctagggggg agttgacctc ctcccccgac cggggttgac
     2881 cgccccggtt caccgcttca cccgctctca ggccgcctgg agcgctgcga tcacgtcgtc
     2941 gccgtagcgc tcgagcttcg tcggcccgat cccgggcacg tcggcgagct cgtgacggct
     3001 cgacggtcgc tgcgcggcga tcgcggcgag cgtcgagtcg tggaagacga cgtaggccgg
     3061 cacgccgtcg gccttcgccc gctcgaggcg ccacttcctg agcgcgcgca cctcgggcgt
     3121 gtcctcgagc ggcgtcttct cgcgccgcgc ggtggcccgc tcgtcggcga gcccgagctc
     3181 gacgaggaac cggctcggct tcgcgtccac cgaccgcgtg aggtagaggc gccgcttcgc
     3241 gcgcgtgatc ccgacgtaga gcaggcggcg ctcctcgtcg atctccccgt cgctcttcgc
     3301 ctggcggatc ggcagctcct tctcctcgag gcgcggcagg aacacggcct cgaactcgag
     3361 ccccttcgcc cggtggtacg tgaggagctg cacgccgcgg tcggcgacgg agtccggccc
     3421 gaaccgctcc tcgagctccg cccagaagcc ggcgatcgtc cgcacgccgt cgtccaggcg
     3481 ctcggcgagg tcgacgaact gcgcgaagtc ggcctggcgg acgagctcct gctcgccgag
     3541 ctccttctcg ggcgcgggca cccagccctc ctccccggcg gccgcgcgga ccgaggccgc
     3601 caccgcggtc gagcccgagg cgttccgcag cctggcccgc agccgccgcg ccgcctggcg
     3661 gagcaggaag ccgccctcgc gcacctggta cgggatcccg gcctcggtga acgcctgctc
     3721 gaagccggcc gagcgcgcgt tcgtccggta gaggaccgcg atctcgccca gcgggacgcc
     3781 ctccgcggcg agctcccgca cccgttcgac gacgaagcgc gtctcctcgg gcccgtcgag
     3841 cgcctcggcc gtcggctccg gcccgtcggg gagcacggcg cggagcgcct tctccgcccc
     3901 gccgagccgc ggcgcgagcc ggttcgcgag ctcgagcacc tgcggggtcg agcggtagtt
     3961 ctcctcgagc cgcacgaccg tcgcgtgcgg ataccgctcc ggcatcccga gcaggtgcgc
     4021 gggcgtcgcg ccggtgaacg agtagatcgc ctggtggtcg tccccgacga cgcagagctc
     4081 gtcgcggccg ccgagccaga ggtcgagaag cgtctgctgg aggaggttga cgtcctggta
     4141 ctcgtccacc gtgatcgccc cgatcgtccg gtggaagcgc tcgcgcgcga cctcgtcctc
     4201 ggcgaagagc cggatcgcca gctcgagcac gtcctcgaag tcgaccttcc cgagcgcctc
     4261 cttgcgccgc tcgtagccct ggaagacgcc gaccatgagg tcgggcggaa gcggggcgcg
     4321 gcgccccatc gccgcctccg tgtacgtcgc gggcgtcagc cggcggttct tcgcccactc
     4381 gatctcggtc gcgaggtcgc cgacggggcg gaagcgatac ggcttgggaa gcccgcgcgc
     4441 gacgttcgtg agcacgatcg ccttcgacgg gaggatcccg cccggcggcg ccgcgccgag
     4501 cgcgtggagc tggccgagcg cggcggcgtg gaacgtccgg gcgcgcaccc ccgtcgcgcc
     4561 gagcgcctcg agccgcgagc gcatctcgcc ggcggccttg tcggtgaacg tgaccgcgag
     4621 gacggactcc gcgcggaacg cgccggtcgc cacctggttc gcgatccgcc gggtgatcgt
     4681 cgtcgtcttg cccgagcccg cgcccgcgag gatgcagacg ggcccgcgca ccgcctcgac
     4741 ggcgcgccgc tgctccgggt tcaggcccgc gaagatccgc tccgagtcca tcgcgcccag
     4801 gctagtgccc gcgtcggcgg gcgaggcccc cgggcgcgcc tttcacgcgt cttccggcac
     4861 agaaccggca cgaacgagcg ccgctcacgc cgccgagacg agaaaaggac ctgaacgact
     4921 agggcgagag caccaccttc acgcagtcgt cctgctttcg cttgaagagc tcgtagccct
     4981 cgggcgcgtc gtcgagcgcc atgcggtgcg tcacgacgaa gctcgggtcg atctcgccgt
     5041 tctcgatccg ctcgagcagc ggccggaggt accggtgcac gtggcactgg cccgccttga
     5101 tcgtgagcga cctgttgacg acggcgccca ttggaaactt gtcgacgagg ccgccgtaga
     5161 cgccgacgac ggacacgatc ccgccgttct tcacgctcat gatcgcctcg cggagggcgt
     5221 gcgcgcggtc ggactcgagc cgggtcgcct gcttcacccg gtcgtacgcg tggagcggga
     5281 gcgacgcgtg gtgcgcctcc atcccgaccg cgtcgatcgc cgcgtcgggg ccgcgcccgc
     5341 cggtcagctc cttgagcgcc tcggggacgt cctcctgctc gtagttcagc gtctccgcgc
     5401 cgctcacctc ggccgccatc cgcaggcgct cggggaagcg gtcgatcgcg atcacgcgct
     5461 cggcgccgag catgaacgcg ctcgcgatcg cgaactggcc gaccgggccg gcgccccaga
     5521 ccgcgacgac gtcgccctgc gtgatgtcgc agagctccgc gcccatgtag cccgtcggga
     5581 ggatgtccga gaggaagagc acctgctcgt ccgggatccc gttctcgagc ttcagcgggc
     5641 cgacgtcggc gaagggcacg cgcacgtatt ccgcctgccc acccgcgtag ccgccggtca
     5701 ggtgcgagta gccaaagatt ccgcacgggg agtggcccca gagcttctcc gcgagccccg
     5761 cgttcgggtt cgagttctcg cagagcgacc accattcggc gcggcacgcg ttgcagttcc
     5821 cgcacgcgat cgggaacggg acgacgacgc ggtcgccgac gcccaggttc gagacgcctc
     5881 gcccgacctc gacgacctcg cccatgaact cgtggccgag gatgtcgccc ttcttcatcg
     5941 tggggacgta gccgtcgtag agatggaggt cggaaccgca gatcgccgtc gaggtgacgc
     6001 ggacgatcgc gtcgcggtcg ttcaggatct gcgggtcggg gacgttctcg acctcgacgc
     6061 tgttcctgcc ggtccagcag acggccctca tcggacggcc tcctcgagcg gccgcgcggg
     6121 gcgctgtcgc agcgtgcgtg acgcgtcgat cccgcccggt acggcgtcgg agcgcgcgat
     6181 ctcgccggtc tcgaggatct gcttgaagcg gcgcaggtcg tccttcgcct gctgcgtcgg
     6241 ctcccggccc atgagcttcg cgaggagcgt ccccacggag cccgcgggcg cctcgtagcg
     6301 aagctcgagc tggatctcgg tgccgcgtcc gccgggcgcc ggcgagaagc gcacggtgcc
     6361 ggaggtctcg acgtcgccgc cgggaagcga gcgccacgcg atccgctcgc ccggcacgtc
     6421 ctcgaccgtc tccgcgtccc actcgaccgt gccggtcggg gacgcgacgc gccagtgcgt
     6481 cgtcccgtcc tcgcgttcct cgaccgactc gacgtgcgcc atgaagcgcg ggaagctcgt
     6541 gacgtcgcgc cagagggcgt acgccgtccc ggtgtcctcg acgacggtga ccgccgctcg
     6601 cacctgcatc ggctcctcct tcgtctcacg gcgcaggccc cggccggccg cgtacgcgag
     6661 gccgagcccg aggccgaacc agccggctcg ccgcagcgtc cccatgccac ctcccgtctc
     6721 ccctcgccgc gggcctaaac tccggagcct cgtgttcgac cacgtgacga tccgcgcctc
     6781 cgaccgaacc gcgtccgagc ggttctacga cctcgtcctc ggggtgctc
//
LOCUS       DASTFC010000060         4869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_940507,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000060 DASTFC010000000
VERSION     DASTFC010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940507"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFO88_01760"
     CDS             complement(<1..743)
                     /locus_tag="VFO88_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9544281.1"
                     /translation="MAVDLFAGSPVRDYEQAVAWYERLLGSEPSFLPHATEAVWELAE
                     HRFLFVVERPEHAGHAVHTIFVDDLDATVAEIAARGIEPAERETYSNGVRKATYRDPD
                     GNEIGLGGAPLAAAAETDVEDAPVSDTLADIPTAAVPYADARWQPIRNHFGLTAFGIN
                     AYTGLRAGDRIIGEHDHSSPEDEQHQELYFVHSGRARFEVDGAVVDAPAGTFVSARDV
                     ATRRSAIAEEDNTVVLVVGAEPGAPFEITP"
     gene            931..2472
                     /locus_tag="VFO88_01765"
     CDS             931..2472
                     /locus_tag="VFO88_01765"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET9544282.1"
                     /translation="MAVMALAAAALVPAGEAMAAPPQKACDTRTNNTYTKLLECIRLD
                     EVREHQAALQAIADENGGNRFSGTPGYDASAAYVAERLEAAGYDVEIQTFDYLAFEVV
                     GPSTLQQIAPNPTTYVEGVDFGVITQSDPGDVTAPVTPVDLQLGLGNTSTSGCDASDW
                     VGFPAGHIALLQRGLCTFELKAENAAAAGAVGIVIFNQGDTTDPSRQGIPAVTLTANN
                     TSGIPVLGTTYALGAQLAGTPGLVMRVFANTLRQILPTSNVIAEKTGVNDDNVVMAGA
                     HLDSVLAGPGINDNGSGTAAILEAAEQLAKLNPQNTLRFAFWGAEESGLVGSTNYVNG
                     LSQAEKDRIALYLNFDMVGSPNYIFMVYDGDQSSFPPPAGVPIPDGSVAIEDIFESFY
                     TLRGEPYDDTQFSGRSDYQAFILNNIPAGGLFTGAEVPKTAEQAAIWGGTVGAQFDPC
                     YHEACDTFANNNDHALDVNADAIAFAILTYAYSTESVNGVPGRKVPGNFQIPAPAGPE
                     GTFVP"
     gene            2541..3341
                     /gene="dapB"
                     /locus_tag="VFO88_01770"
     CDS             2541..3341
                     /gene="dapB"
                     /locus_tag="VFO88_01770"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET9544283.1"
                     /translation="MIAVCIAGATGWTGRALVPAVLAADDLRLVSAVARSAAGDDLGE
                     ALGGRTIGVPVRATVAEALDGVDVLVDYTSAAAVRTNVLAAVEAGVGVVVGSSGLTAG
                     DFEEIDALARERGVGVVASGNFSVTAAMCQAAALLAARHLPRWEIVDYASAGKPDVPS
                     GTARELAERLGEVRRPEVGHPLDELHGPREARGATVGGAQVHSVRLPSFVVSTEIVFG
                     GEDERLTIRHDAGGSPRPYVEGTLLAVRRVPGLVGLIRGLDTLLATSG"
     gene            complement(3338..3997)
                     /locus_tag="VFO88_01775"
     CDS             complement(3338..3997)
                     /locus_tag="VFO88_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9544284.1"
                     /translation="MSDSPATPRVVHLDELPRVANGGLWRPVRRELGVTGFGVNAYTA
                     EAAGDELIEPHDETSPGSGRHEELYLVLEGSARFRVGDEEVDAPTGTFVFVPPGVHRE
                     ATAASAPATVVVVGGRPGDALPVSPFEYWYAAEPAYQAGDFDEAVRIASEGLETYPEH
                     PHLNYQLACYEALAGRPEAALARLEIACREERVRGWAADDEDFASLRGDERFQRLLGA
                     S"
     gene            complement(4028..4606)
                     /locus_tag="VFO88_01780"
     CDS             complement(4028..4606)
                     /locus_tag="VFO88_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544285.1"
                     /translation="MADEAAVDLFWLPLGAGGHSVRLNGRVFEAVAARLAHRTRCDLY
                     HSALQVHVPEGRYAIEVTPVFSGDAAERGVVAGGAVGARAAGRLRIFRDEVRRWLGGE
                     IPDLAEAVESPQRLAGDLATARRVLELVPRAPTPVWGRDELRAGEMWNSNSVVSWLLV
                     QAGLPPESARLPARGRAPGWDAGIVVARRSAA"
BASE COUNT          671 a         1768 c         1803 g          627 t
ORIGIN      
        1 ggtgtgatct cgaacggcgc gccgggctcc gccccgacga cgagcacgac cgtgttgtcc
       61 tcctcggcga tcgccgaccg cctcgtggcg acgtctcgcg cgctcacgaa cgtgcccgcc
      121 ggggcatcga cgaccgcgcc gtcgacctcg aacctggctc ggccggagtg gacgaagtac
      181 agctcctggt gttgctcgtc ctcggggctc gagtggtcgt gctccccgat gatgcggtcg
      241 ccggcacgca gccccgtgta ggcgttgatg ccgaacgccg tgagcccgaa gtggttgcgg
      301 atcggctgcc agcgggcgtc ggcgtacgga acggccgccg tcgggatgtc ggcgagcgtg
      361 tcggaaaccg gagcatcctc cacgtcggtc tcggcggcgg ccgcgagcgg agcgccgccg
      421 aggccgatct cgttgccgtc ggggtcgcgg tacgtggcct tgcggacgcc gttcgagtag
      481 gtctcccgct cggccggctc gattccccgg gcggcgatct cggcgacggt cgcgtcgagg
      541 tcgtcgacga agatcgtgtg gacagcgtgc cctgcgtgct cgggccgctc gacaacgaac
      601 aggaaccggt gctcggcgag ctcccacacc gcctcggtcg cgtggggcag gaagctcggc
      661 tcggagccga ggaggcgctc gtaccaggcc accgcctgct cgtagtcgcg caccgggctg
      721 ccggcgaaga gatcgaccgc catgcgcgcg accctaccgc cgagagctgc gtcctggggg
      781 ctgccagggc ttcggtgctc ggcggcgagg gggtatgctc cgcgccctgc ggccgggcgg
      841 ctcacactcg ccgaccggcc ggaaccgaga gtgaggggag cacacatgtc gggacttgca
      901 ggagcgctcg ggcgcagagg cgtccggcgc atggcggtca tggccctcgc ggcggcggca
      961 ctcgtgcccg ccggggaggc gatggcggcg ccaccacaga aggcgtgcga cacacgcacg
     1021 aacaacacgt acacgaagct gctcgagtgc atccggctcg acgaggtgcg tgagcaccag
     1081 gcggcgctcc aggcgatcgc ggacgagaac ggcgggaacc gcttctccgg cacgccgggc
     1141 tacgacgcct ccgccgccta cgtggccgag cggctcgagg cggccggcta cgacgtggag
     1201 atccagacgt tcgactacct cgcgttcgag gtcgtcggcc cgtcgaccct ccagcagatc
     1261 gctccgaacc cgacgacgta cgtcgagggg gtcgacttcg gcgtcatcac tcagagcgat
     1321 ccgggtgacg tcacggcgcc ggtcacgccg gtcgacctcc agctcgggct gggcaacacg
     1381 tcgacgagcg gctgcgacgc ctccgactgg gtcgggttcc cggccgggca catcgccctg
     1441 ctccagcgag ggctctgcac attcgagctc aaggccgaga acgccgcggc cgccggggcg
     1501 gtcgggatcg tgatcttcaa ccagggcgac acgaccgatc cgagccggca ggggatcccg
     1561 gcggtgacgc tcaccgccaa caacacgagc ggcatcccgg tgctggggac gacgtacgcg
     1621 ctcggcgcgc agctcgcggg cactccgggg ctcgtgatgc gcgtcttcgc gaacaccctg
     1681 cgccagatcc tgccgacgtc gaacgtgatc gccgagaaga ccggcgtgaa cgacgacaac
     1741 gtggtcatgg ccggcgcgca cctcgactcg gtgctcgcag ggcccgggat caacgacaac
     1801 ggctcgggca ccgcggcgat cctcgaggcc gccgagcagc tcgccaagct gaacccgcag
     1861 aacacgctcc gcttcgcgtt ctggggcgct gaggagagcg gtctcgtcgg ctcgacgaac
     1921 tacgtgaacg gcctgagcca ggcggagaag gacaggatcg cgctctacct gaacttcgac
     1981 atggtcggct cgccgaacta catcttcatg gtgtacgacg gcgaccagtc gagcttcccg
     2041 ccgccggcgg gcgttcccat cccggacggc tcggtcgcga tcgaggacat cttcgagtcc
     2101 ttctacacgc tccgcggcga gccgtacgac gacacgcagt tcagcggacg cagcgactac
     2161 caggcgttca tcctgaacaa catccccgcc ggcgggctgt tcaccggcgc ggaggtcccg
     2221 aagaccgccg agcaggcggc gatctggggc ggcaccgtgg gcgcgcagtt cgacccgtgc
     2281 taccacgagg cgtgcgacac gttcgcgaac aacaacgacc acgcgctcga cgtcaacgcc
     2341 gacgcgatcg cgttcgcgat cctcacgtac gcgtactcga cggagtccgt gaacggggtg
     2401 ccgggcagga aggttcccgg caacttccag attccggctc ctgccggccc cgagggcacc
     2461 ttcgtcccgt agccggacgc gcaggacgga gggggcggcc ggaccggccg ccccctcccc
     2521 tgcgggatag gctgccgcgc gtgatcgccg tctgcatcgc gggagcgact ggctggacgg
     2581 ggcgcgcgct cgtccccgcg gtgctcgccg cggacgacct gcggctcgtg tccgcggtcg
     2641 cgcgctcggc cgccggcgac gacctcggcg aggcgctcgg cggccgcacg atcggggtgc
     2701 cggtgcgggc gacggtggcc gaggcgctcg acggcgtcga cgtgctcgtc gactacacct
     2761 cggcggcggc ggtgcggacg aacgtcctcg cggcggtcga ggcgggtgtc ggcgtggtcg
     2821 tggggagctc ggggctcacg gccggcgact tcgaggagat cgacgcgctc gcccgtgagc
     2881 gcggggtcgg cgtcgtcgcg tccgggaact tctccgtcac cgcggcgatg tgccaggcgg
     2941 cggcgctcct cgcggctcgg cacctcccgc ggtgggagat cgtcgactac gcgagcgccg
     3001 gaaagcccga cgtgccgagc gggacggcgc gcgagctcgc ggagcggctc ggcgaggtgc
     3061 gccggcccga ggtcgggcat ccgctcgacg agctgcacgg cccgcgggag gcacgcggcg
     3121 cgacggtcgg cggggcgcag gtccactccg tgcggctgcc gagcttcgtc gtctcgaccg
     3181 agatcgtgtt cggcggcgag gacgagcgcc tcacgatccg ccacgacgcg ggcggctcgc
     3241 cccggcccta cgtggagggg acgctgctcg ccgtccgccg cgtccccggg ctcgtcgggc
     3301 tgatccgcgg gctcgacacg ctcctggcga cgtccggcta ggacgcgccg agcaggcgct
     3361 ggaagcgctc gtcgccgcgc aggctcgcga agtcctcgtc gtcggccgcc cacccgcgga
     3421 cgcgttcctc gcggcaggcg atctcgagcc gggcgagcgc ggcctccggc cgtccggcga
     3481 gcgcctcgta gcacgcgagc tggtagttca ggtgcgggtg ctcggggtac gtctcgagcc
     3541 cctcgctcgc gatccggacg gcctcgtcga agtcgcccgc ctggtaggcg ggctcggcgg
     3601 cgtaccagta ctcgaagggc gagacgggga gcgcgtcgcc cggccgcccg ccgacgacga
     3661 cgaccgtcgc cggggccgac gcggcggtcg cctcgcggtg cacgcccggc ggcacgaaga
     3721 cgaacgtccc ggtcggggcg tcgacctcct cgtcgccgac ccggaagcgg gcgctcccct
     3781 cgagcacgag gtagagctcc tcgtgccggc ccgagcccgg gctcgtctcg tcgtggggct
     3841 cgatgagctc gtcgccggcg gcctcggcgg tgtacgcgtt gacaccgaag ccggtcacgc
     3901 cgagctcgcg ccgcacggga cgccagaggc ccccgttcgc gacgcgcggg agctcgtcca
     3961 ggtgcacgac cctcggggtc gcgggcgagt cgctcacgca cgcagcgtac cgcgccgcga
     4021 cggctggtca ggcggccgag cggcgggcga cgacgatccc cgcgtcccag ccgggcgcac
     4081 gtcctcgcgc gggaagccgg gccgactcgg gcgggagccc ggcctgcacg aggagccacg
     4141 acacgaccga gttcgagttc cacatctcgc cggcgcggag ctcgtcccgg ccccagaccg
     4201 gcgtcggcgc ccgcgggacg agctcgagca cgcgccgcgc cgtcgcgagg tcgccggcga
     4261 gccgctgcgg gctttcgacc gcctcggcga ggtccgggat ctcgccgccg agccagcggc
     4321 gcacctcgtc gcggaagatc cggagccggc cggccgcacg tgcgccgacc gcgccgcccg
     4381 ccacgacgcc gcgctcggcc gcgtcgcccg agaagaccgg tgtcacctcg atcgcgtagc
     4441 ggccctccgg gacgtgcacc tggagcgccg agtggtagag gtcgcagcgc gtccggtgtg
     4501 cgagccgggc ggcgaccgcc tcgaacacgc ggccgttcag ccgcaccgaa tggccgcccg
     4561 ccccgagcgg aagccagaag agatcgacgg ccgcctcgtc cgccatgccg cgagtgtgcg
     4621 cccgccgcgc gtgcgcggca tccgcgcgcc accggatccg cgctgcggcc gactaccgac
     4681 ccgtttgccc gcgaggccgg tgggtacgcg accccctcgt cgtcagaccg aaggaggccg
     4741 cagatgatcc tgcgttacga ccgaatcggg atggagatcc cgccgccgtc cgacccggat
     4801 cccgccggcg ccgccgccgt gcaggagctg atgggcgggc gcttcggcga gatgtccacg
     4861 ttcatgaac
//
LOCUS       DASTFC010000061         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1055874,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000061 DASTFC010000000
VERSION     DASTFC010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055874"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1546)
                     /locus_tag="VFO88_01785"
     CDS             complement(299..1546)
                     /locus_tag="VFO88_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HET9544286.1"
                     /translation="MVALALTGTASAQGTGSSQKPTRHAPCVQGEAAGFPCKRVDLLS
                     YFWMEEIGGSQVVGQGRGSDVWGWEDPETGREYVIAGRENGTAFVDITDPKRPLYLAN
                     LPTASPQNQVIWRDIKVYADHAYIVSEASNHGMQIFDLNRLRTLDRSAAPVNVVEDAR
                     YTGFGRAHNLAINEETGYGYAVGTRQGIDCSQGMHVMDLSTPTAPEFAGCVSEDGYIH
                     DTQCVVYRGADARYLGREICFAASPTPGPSPHSVLIVDVTDKANPLIISNTPYEGAGY
                     SHQGWLTPDQETFVFGDEGDEGAFGHNTRTLVFDVRNLESPLHVGTHFGPTRAINHNI
                     YTENRWVYQSNYTAGLRILDGRDAADGELEEVAYFDVYPPHDNAVFGFGTWSNYAWFS
                     SGVIAVHGYQGLWIVKARLGRQS"
     gene            1794..2786
                     /locus_tag="VFO88_01790"
     CDS             1794..2786
                     /locus_tag="VFO88_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET9544287.1"
                     /translation="MARPTLPRPRIRRARAAAGDVASRPEPRIAELAAGGLTWVHVSH
                     PTADDAAELASRFGFHELDVEDVLSKRQRPKIDEYPDYLFLVLHFPFYDKAIQRLNAA
                     ELDVFVGHDFLITIPNVELHPVTYLQRRCDEDPELRAELFAKGPGYLLYHILDDLFDY
                     CFPILDKISHKLDRIEDEMHEGRSEEIVRDISNAKQEIIAYRKIIKPERSTLRALERH
                     TERFLHEDLEVYYDDLVDAAERIWDLLDNYKEVVDGLEQTNESVISHKQNAVLRVLTV
                     IATIMLPLTLVTGIFGMNVAVPGEGASGPFWAIIAVLVALGVAMLGYFRLRKWL"
     gene            2797..3282
                     /locus_tag="VFO88_01795"
     CDS             2797..3282
                     /locus_tag="VFO88_01795"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9544288.1"
                     /translation="MRRLWAPWRLEYVSTADEQDGCVFCRAASSGSDEDALVVHRGDR
                     AFVLVNKFPYASGHLMVAPYRHEGELGELADDEALEIHRLASVALGALAETMHPQGFN
                     LGWNLGRIAGAGVVDHVHLHVVPRWAGDTNFMPVLADVRVLPEALEATRAKLASAWPA
                     S"
     gene            3423..>4070
                     /locus_tag="VFO88_01800"
     CDS             3423..>4070
                     /locus_tag="VFO88_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544289.1"
                     /translation="MALDPKVFKAYDVRGIHPTELDEDGAYRIGRAYAEVFEPRRIAV
                     GRDMRLSAPTMAEAVIRGAADAGVDVLDIGMVGTEMVYFAVGEHGLDGGIAVTASHNP
                     KEYTGMKIVRRGALPVGGDSGLLEIRDRAEHDPGEPAPAAERGAVEKADVYPGFVERV
                     LSFVDVDAIRPLRVVIDAANGMAGAMLPPILDRLPVEAVRCYFDPDGSFPNHEPNP"
BASE COUNT          575 a         1472 c         1408 g          615 t
ORIGIN      
        1 ggtgccaggc accggccgtg tcggcggggg ccgtggccgc ttgcgccgtg tccggtgcca
       61 ggcaccggcc gtggctcgag gagacgaccg gccgggccca ccgacaagcg aaggatggga
      121 ggaagcccgc gcgaatccgg cccgcgttgc gcgcgggccg gatgattcgc gcaggcttct
      181 gcggcgttcc gacagacgtc cgctccggac acggccggtg cctggcaccg gacaatgtcc
      241 cgaggcgacg gggggcccgc tcgcgcgggc cccccggtcg gtcgaaagcg acctccgcct
      301 agctctgccg gccgaggcgg gccttcacga tccacaggcc ctggtagccg tgcacggcga
      361 tgacgccgct cgagaaccac gcgtagttgc tccacgtgcc gaagccgaag acggcgttgt
      421 cgtggggcgg gtacacgtcg aagtacgcga cctcctcgag ctccccgtcg gcggcgtcgc
      481 gcccgtcgag gatccggagc ccggccgtgt agttcgactg gtagacccag cggttctccg
      541 tgtagatgtt gtggttgatc gcccgggtcg gcccgaagtg cgtgccgacg tgcagcgggc
      601 tctcgaggtt ccggacgtcg aagacgagcg tccgcgtgtt gtgcccgaac gcgccctcgt
      661 caccctcgtc tccgaagacg aacgtctcct gatcgggcgt cagccagccc tggtgggagt
      721 agcccgcgcc ctcgtacggc gtgttcgaga tgatcagcgg gttcgccttg tccgtcacgt
      781 ccacgatcag cacggagtgc gggctcggcc ccggcgtcgg gctcgcggcg aagcagatct
      841 cgcgcccgag gtaccgcgcg tccgccccgc ggtagacgac gcactgcgtg tcgtggatgt
      901 agccgtcctc gctcacgcag ccggcgaact ccggcgcggt cggcgtcgag agatccatca
      961 cgtgcatgcc ctggctgcag tcgatcccct ggcgcgtccc gaccgcgtag ccgtagccgg
     1021 tctcctcgtt gatcgcgagg ttgtgcgcgc gcccgaaccc ggtgtagcgc gcgtcctcga
     1081 caacgttcac cggcgcggcg gagcggtcga gcgtccgcag ccggttcagg tcgaagatct
     1141 gcatcccgtg gttcgaggcc tccgagacga tgtacgcgtg gtccgcgtag accttgatgt
     1201 cccgccagat cacctggttc tgcgggctcg cggtcggcag gttcgcgagg tagagcggcc
     1261 gcttcgggtc ggtgatgtcg acgaacgcgg tgccgttctc ccggccggcg atcacgtact
     1321 cgcgcccggt ctccggatcc tcccagcccc acacgtcgct cccgcgaccc tggccgacga
     1381 cctggctgcc gccgatctcc tccatccaga agtacgacag caggtcgacg cgcttgcacg
     1441 ggaagcctgc ggcctcgccc tgcacgcacg gcgcgtgccg ggtcggcttc tgcgagctgc
     1501 ccgtcccctg cgccgacgcg gttccggtca gcgcgagcgc gaccacggcc gccaacgctg
     1561 tgatcagcct gactctcatg ctcgattccc ccttgtcctg gtcggggctt tgcccccgcg
     1621 cgccgccggg tggaggtccg caaccgggcc tttcgacccg cctccgtcgc cggaggcacc
     1681 ctgggcacga ccgcgccagc atacgggacc gcgggcggcc gcgcggaggg ctcggcctgc
     1741 gtgcggacta gactcagcgg ggatgacggg aggcgaggag ccccgaccac ccgatggcac
     1801 gcccgaccct tcccaggcct cgtatccggc gcgcgcgcgc cgcggccggc gacgtcgcgt
     1861 cgcgccccga gccgcggatc gccgagctcg cggccggcgg gctcacgtgg gtgcacgtat
     1921 cgcacccgac ggccgacgac gcggcggagc tcgcgtcgcg cttcgggttt cacgagctcg
     1981 acgtcgagga cgtcctctcg aagcgccagc gcccgaagat cgacgagtac ccggactacc
     2041 tcttcctcgt cctccacttc cccttctacg acaaggcgat ccagcggctg aacgcggcgg
     2101 agctcgacgt gttcgtcggg cacgacttcc tcatcacgat cccgaacgtc gagctgcacc
     2161 ccgtcacgta cctccagcgg cgctgcgacg aggacccgga gcttcgggcg gagctcttcg
     2221 ccaaggggcc gggctacctg ctctaccaca tcctcgacga cctcttcgac tactgcttcc
     2281 cgatcctcga caagatctcg cacaagctcg accgcatcga ggacgagatg cacgaggggc
     2341 gctccgagga gatcgtccgc gacatctcga acgcgaagca ggagatcatc gcgtaccgga
     2401 agatcatcaa gccggagcgc tccaccctcc gcgcgctcga gcgccacacc gagcggttcc
     2461 tccacgagga tctcgaggtc tactacgacg acctggtcga cgccgccgag cggatctggg
     2521 acctcctcga caattacaag gaggtcgtgg acgggctcga gcaaacgaac gagtcggtca
     2581 tctcgcacaa gcagaacgcc gtcctgcgcg tgctaaccgt gatcgcgacg atcatgctcc
     2641 ccctcaccct cgtcaccggg atcttcggga tgaacgtggc ggtgccgggc gagggcgcgt
     2701 ccgggccgtt ctgggcgatc atcgccgtgc tcgtcgcgct cggggtcgcg atgctcggct
     2761 acttccggct gaggaagtgg ctctgagggc ccgcgcgtga ggcgcctctg ggcgccgtgg
     2821 cggctggagt acgtctcgac cgcggacgag caggacggct gcgtcttctg ccgcgcggcg
     2881 tcgtcgggct cggacgagga cgccctcgtc gtccaccgcg gcgaccgtgc gttcgtgcta
     2941 gtgaacaagt tcccgtacgc gtccgggcac ctgatggtcg cgccctaccg gcacgagggc
     3001 gagctcggcg agctcgcgga cgacgaggcg ctcgagatcc accggctcgc gtcggtcgcg
     3061 ctcggcgcgc tcgcggagac gatgcacccg caggggttca acctcggctg gaacctcggg
     3121 cggatcgccg gcgcgggcgt cgtcgaccac gtccacctgc acgtcgtgcc gcgctgggcc
     3181 ggcgacacga acttcatgcc ggtgctcgcg gacgtccgcg tgctgcccga ggcgctcgag
     3241 gcgacgcgcg cgaagctcgc ctccgcgtgg cccgcgtcct agcgcttcac gcacgcgggt
     3301 aacgggaggt cggcccccac ccctacgatt catcgtatcg gcgcaaggcg cacgtgtctc
     3361 gcgcgcttcg tgccggatgt gtgacggctt cgtgccggga ctgcgcctag gatgaccgcg
     3421 ccatggcgct cgacccgaag gtcttcaagg cgtacgacgt ccgtgggatc cacccgacgg
     3481 agctcgacga ggacggcgcg taccggatcg gccgcgcgta cgcggaggtc ttcgagccgc
     3541 gccggatcgc ggtcggccgc gacatgcggc tctccgcgcc gacgatggcg gaggcggtga
     3601 tccgcggcgc cgcggacgcg ggcgtcgacg tcctcgacat cgggatggtc ggtaccgaga
     3661 tggtctactt cgccgtcggc gagcacgggc tcgacggcgg catcgcggtc accgcgtcgc
     3721 acaacccgaa ggagtacacg gggatgaaga tcgtccgccg cggggcgctc cccgtgggcg
     3781 gcgactccgg gctcctggag atccgcgacc gcgccgagca cgaccccggc gagccggcgc
     3841 cggccgccga gcgcggcgcc gtggagaagg cagacgtcta ccccggattc gtcgagcgcg
     3901 tcctctcctt cgtcgacgtg gacgcgatcc gaccgctgcg cgtcgtcatc gacgccgcga
     3961 acggcatggc cggcgcgatg ctcccgccga tcctcgaccg cctgcccgtc gaggccgtcc
     4021 gctgctactt cgacccggac ggaagcttcc cgaaccacga gccgaacccg
//
LOCUS       DASTFC010000062         8121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_693310,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000062 DASTFC010000000
VERSION     DASTFC010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693310"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VFO88_01805"
     CDS             complement(<1..1211)
                     /locus_tag="VFO88_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9544290.1"
                     /translation="MRKVVIMGAGGRDFHNFNVAFRGDPEAKVVAFTAAQIPGIDDRV
                     YPASLAGDGYPDGIPIRPEEELTALVREHEVDEVVLAYSDLSHEEVMHKASIALAAGA
                     DFALMGPKATMLGSQKPVVAVCAARTGSGKSQTSRRVGQLLVDAGLSVALVRHPMPYG
                     DLEAMRVQRFATLEDIDASSPTIEEREEYEAPVELGMIMYAGVDYGEILAAAQAEADV
                     VVWDGGNNDFPFFRPDLFITVVDPLRPGHELRYHPGEANVRMADVVVVNKVDSAEEAA
                     VGQVVGNVLAINPAATIVLAESPVTLGDGPSLEGKRVLVVEDGPTITHGGRPYGAGTV
                     AAKAAGAAELVDPRPFAVGSIAETFERYPEIGAVLPAMGYGDEQLAELAATIDAADCD
                     VVVTGTPMNLTR"
     gene            complement(1282..2697)
                     /locus_tag="VFO88_01810"
     CDS             complement(1282..2697)
                     /locus_tag="VFO88_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9544291.1"
                     /translation="MSTVTPTGLAPVKTLPDEARVPPRPALKRSMGLWMATALVIGNM
                     VGSGVFLLPAAMAGTAGPVSVLAWVFTGLGAMLLALVFATLGRRYTKTGGPYVYARKA
                     FGDFVGFQTAWSYWINAWVGNAAIAIAFAAYLSVFWGDATKNWVAALLAIGLVWLLTL
                     VNILGVREAGWIQVVTTVLKFTPLLVIGVVGLFFMDSANFDPFTVDQSWDFGIGAAAL
                     LTLWAFIGLESATVPAEEVKDPEKTIPRATIIGTAAAALLYLVATLALFGMIPSAQLA
                     ESASPFADGANEIFGGTWGGKAIAVVAMISIFGVLNGWILLQGRAPLAPARDGLFPRR
                     FARVDGKRGTPVFGLVVSSVLVTALLMTNFQTEGSVVDTFTDIIIIATLTALVPYLYS
                     SAAQLYLFFADRAAFSGVKLARHSTIALLAFGYSLWAIWGAGYESLAKGFMLLLAGIP
                     VYLWVKWRKSVREPGSVRTEA"
     gene            complement(2694..2969)
                     /locus_tag="VFO88_01815"
     CDS             complement(2694..2969)
                     /locus_tag="VFO88_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544292.1"
                     /translation="MGVHAHARHLFDEGWSRAYWLGRCEGFHVEGPRGRVGVVEAVRR
                     GGDGSPTSLDISCGVLRRRVREVPIDDIAEIDPRDLRIVLRREKEES"
     gene            complement(3045..5669)
                     /locus_tag="VFO88_01820"
     CDS             complement(3045..5669)
                     /locus_tag="VFO88_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HET9544293.1"
                     /translation="MALGQAVRAPPAGLTSEEARLRFADAGPNALAEPRRPSHARRFA
                     AQLVHLFALLLWTGSALAWLAGMPELSVAIAAVVLVNAVFAFAQEYRAERAVAALRSM
                     LPPHARVRRDGGVREIAAEEVVPGDLLVLAAGDRVSADAALVVHVGLRVDESALTGES
                     YPVEPEAEVSAGTYVVAGSGEALVTATGMRTRFGRIAELTQATREGRSPLERQLDRLT
                     RFVAVLAVAIGTTFFLVAGALGMPLADRFVFAIGVTVALVPEGLLPTVTLSLALATQR
                     MARRNALVRRLSSVEALGATTVICTDKTGTLTENQMTVRRVWTPEGELEVEGAGYEPF
                     GRFLRDGEVADPRTLAELLRAGLLCNDARLVHGGDGWRVEGDPTEGALVVLAEKGGLR
                     HEQEAAHRPRLAELPFDSRRKRMTTLHLDGDGAGRVAYAKGAPEEIVARSTLGPVERA
                     EALAVAERFEHDALRVLAVARRTVPADGGDTPEELERGLELLGLVGMDDPPRPEVADA
                     VARCRRAGIRIVMVTGDSGATAAAVARQIGLASEPHVVEGGELARLDDEELRTRLAER
                     EVVFARIDPEAKLRLAHVLREEGEIVAMTGDGVNDAPALREADIGVAMGLRGTDVARE
                     AADMVLLDDNFASVVAAVEEGRAVYENIRRFAGYHFCSNVGELVPFVVWGATGGAVPL
                     PLVVMQVLAIDLGTDMLPAIGLGAERAEAGTMERPPRPRRERLLDRATIRRVFGLVGP
                     LEGLAGLASFFAAYALAGWRPWEPLASSGDLYVTATAMTCGGIVAAQVGAGLAFRTSR
                     ESSLSVGLLSNRLLAAGIAFEIALLGALLYAPPLAELFHMAPLGPAELAVLLLWPPLV
                     FGADEARKALSRRSVETT"
     gene            complement(5766..7406)
                     /locus_tag="VFO88_01825"
     CDS             complement(5766..7406)
                     /locus_tag="VFO88_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET9544294.1"
                     /translation="MHRRVLVVLLVLLAALVAPAAHGAPVFVLTGKGWGHGIGLGQYG
                     TQGRALQGWGYEAILEHYYTGASLTSGQPNDQVRVLLASAQSSLSLSSASSFTAGGTT
                     LAAGTYAVTAGTGTVRFTRSGATTTVASPASVAPGAASLGLGGVLYRGSLVFASASGK
                     VSALNVLNRQPYVQGVVPREMPASWHPQALAAQAVAARTYSLGGGHCTWLGSPVYCPD
                     TRDQVYGGRSAETDATNTAVQSTAGEIMTHGGSPIAAYFFSTSGGRTAAKADEWGGAP
                     VPYLVSVLDTPDSISPHHAWGPKDAEIDCAGTSPDCVFGAAKVQTLLGLAQRPVDLEV
                     TARNSSTRVATLVARGSSTTASFSGTTARTELGLRSTWFYVGVLSLKPAATTITYGGS
                     VKLSGLARRGGTAGWGTATLERRRVGETAWETVGEPLPNGAWERVVKPKRANDYRVVS
                     ALANGEAQRIQVRTSVAFAKPVAPFTRLRGIVRPPAAGILVTIGRQRPDGTFARVGTT
                     RTLADGSFSLRISSSGTYRARADAGANLLEGSALVTVP"
     gene            7595..>8121
                     /locus_tag="VFO88_01830"
     CDS             7595..>8121
                     /locus_tag="VFO88_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9544295.1"
                     /translation="MSQEFRALGVSADVVEVLASRDIVSPFSIQTAVLPEALSGGDVL
                     AKSPTGSGKTLAFGLPIVERLRPDEGTPAALVLVPTRELAAQVAEQLEPIGAVKGLRV
                     ATAYGGAPMGPQVKRLKGAHIVVATPGRLQDLVERRHVVLSKVRVLVLDEADRMLDMG
                     FKPQVDRLVRRLPRER"
BASE COUNT         1143 a         2988 c         2964 g         1026 t
ORIGIN      
        1 cgcgtcaggt tcatcggcgt gccggtcacg acgacgtcgc agtcggcggc gtcgatcgtg
       61 gccgcgagct cggcgagctg ctcgtcgccg tagcccatcg ccgggagcac ggccccgatc
      121 tccgggtagc gctcgaacgt ctcggcgatc gagccgaccg cgaacggccg cgggtcgacg
      181 agctccgcgg cgccggcggc cttcgccgcc accgtcccgg ccccgtaggg cctgccgccg
      241 tgggtgatcg tcggcccgtc ctcgacgacg aggacgcgct tgccctcgag cgaggggccg
      301 tccccgagcg tcacgggcga ctccgcgagg acgatcgtgg ccgccgggtt gatcgcgagg
      361 acgttcccga cgacctgccc gaccgcggcc tcctccgcgc tgtccacctt gttcacgacg
      421 acgacgtcgg ccatgcggac gttcgcctcg ccggggtggt accggagctc gtggcccggc
      481 cggagcgggt cgacgaccgt gatgaagaga tccgggcgga agaacgggaa gtcgttgttc
      541 ccgccgtccc agacgacgac gtcggcctcg gcctgcgcgg ccgcgaggat ctcgccgtag
      601 tcgacgcccg cgtacatgat catccccagc tcgacgggcg cctcgtactc ctcgcgctcc
      661 tcgatcgtcg ggctcgacgc gtcgatgtcc tcgagcgtcg cgaagcgctg gacgcgcatc
      721 gcctcgaggt cgccgtacgg catcgggtgc ctgacgagcg cgaccgagag gcccgcgtcc
      781 acgagcagct ggccgacgcg ccggctcgtc tggctcttgc cgctgccggt gcgcgccgcg
      841 cagaccgcga cgaccggctt ctgcgagccg agcatcgtcg ccttcggccc catgagcgcg
      901 aagtccgcgc cggcggcgag ggcgatggac gccttgtgca tgacctcctc gtgcgagagg
      961 tccgagtagg cgagaacgac ctcgtcgacc tcgtgctcgc gcacgagcgc agtgagctcc
     1021 tcctccggcc ggatggggat cccgtccggg tagccgtccc cggcgaggct cgccgggtag
     1081 acgcgatcgt cgatgcccgg gatctgcgcc gcggtgaacg cgaccacctt cgcctccggg
     1141 tcgccccgga acgcgacgtt gaagttgtgg aagtccctgc ctccggctcc catgatcacg
     1201 accttgcgca tgacgtctct cccttcggtc gaggcggggc cggggcccgt cgaggccccg
     1261 gcccggcccg gttgtgtgcg actacgcctc cgtccgcacc gaccccggct cgcgaacgct
     1321 cttccgccac ttcacccaga ggtagaccgg gatcccggcg agcaggagca tgaagccctt
     1381 ggcgagcgac tcgtagcccg cgccccagat cgcccagagc gagtagccga aggcgaggag
     1441 cgcgatcgtg gagtgccgcg cgagcttcac gcccgagaac gcggcccggt cggcgaagaa
     1501 gaggtagagc tgagcggcgg acgagtacag gtacggaacg agcgccgtca gcgtggcgat
     1561 gatgatgatg tcggtgaacg tgtcgaccac cgacccctcg gtctggaagt tcgtcatgag
     1621 cagcgccgtg acgagcaccg aggagacgac gaggccgaac accggcgtcc cgcgcttgcc
     1681 gtcgacgcgc gcgaaccgcc gcgggaagag cccgtcgcgg gccggcgcga gcggcgcgcg
     1741 gccctggagc aggatccacc cgttcagcac accgaagatc gagatcatcg cgacgaccgc
     1801 gatcgccttg ccgccccacg tgccgccgaa gatctcgttc gcgccgtcgg cgaacgggct
     1861 cgcggactcg gccagctgcg cgctcgggat catcccgaag agcgcgagcg tggcgacgag
     1921 gtagaggagc gccgccgccg cggtgccgat gatcgtggca cgcgggatcg tcttctccgg
     1981 atccttcacc tcctcggccg ggacggtcgc cgactcgagg ccgatgaacg cccacagcgt
     2041 gaggagcgcc gccgcgccga tcccgaagtc ccagctctgg tcgacggtga acgggtcgaa
     2101 gttcgcgctg tccatgaaga acaggccgac gacgccgatc acgaggagcg gggtgaactt
     2161 gaggacggtc gtgacgacct ggatccagcc ggcctcacgg acgccgagga tgttgacgag
     2221 cgtgaggagc cagacgaggc cgatcgcgag gagcgccgcg acccagttct tcgtcgcgtc
     2281 gccccagaac acgctcaggt acgcggcgaa cgcgatcgcg atggccgcgt tgccgaccca
     2341 ggcgttgatc cagtagctcc acgcggtctg gaacccgacg aagtcgccga aggccttccg
     2401 cgcgtacacg tacggcccgc cggtcttcgt gtaccggcgg ccgagggtgg cgaagacgag
     2461 cgcgaggagc atggcgccga ggcccgtgaa gacccaggcg aggacggaca cggggcccgc
     2521 cgtcccggcc atcgcggccg gcagcaggaa gacgcccgat ccgaccatgt tcccgatcac
     2581 gagggcggtc gccatccaca ggcccatcga gcgcttgagc gcgggacggg gtgggacgcg
     2641 cgcctcgtcg ggaagcgtct tgaccggcgc caggccggtc ggtgtgacgg tgctcatgac
     2701 tcctccttct cccggcggag gacgatccgc aggtcgcgcg ggtcgatctc cgcgatgtcg
     2761 tcgatcggga cctcgcggac gcgtcggcgc aggacgccgc agctgatgtc aagcgacgtg
     2821 ggcgagccgt cgcctcccct tcggacggct tcgacgacgc cgacgcgtcc gcgtggcccc
     2881 tcgacgtgga agccctcgca gcgcccgagc cagtaggccc gcgaccaccc ctcgtcgaac
     2941 aggtgacgcg cgtgcgcgtg gactcccatt gcgtctctcc tttggctcgg gtccatcgtc
     3001 cacccgggac cactgcacgg catcggagag agcgctgaac cgcgctacgt ggtttccacc
     3061 gagcgccggg agagcgcctt gcgcgcctcg tcggccccga agacgagcgg cggccagagc
     3121 aggagcacgg cgagctcggc gggcccgagc ggcgccatgt ggaagagctc ggcgagcggc
     3181 ggcgcgtaga ggagcgcccc gagcagcgcg atctcgaacg cgatcccggc cgcgagcagg
     3241 cggttcgaca ggagcccgac ggagagcgac gactcgcggc tcgtccggaa cgcgagcccg
     3301 gcgccgacct gcgcggccac gatcccgccg cacgtcatcg ccgtcgccgt cacgtagagg
     3361 tctccggagg aagcgagcgg ctcccacggg cgccagccgg cgagggcgta ggcggcgaag
     3421 aagctcgcga gcccggcgag cccctcgagc ggcccgacga gaccgaacac gcgcctgatc
     3481 gtcgcccggt cgagaagccg ctcgcggcgc ggccgcggcg ggcgctccat cgtccccgct
     3541 tcggcgcgct ccgcccccag cccgatcgcg gggagcatgt cggtcccgag gtcgatcgcg
     3601 agcacctgca tgacgacgag cggcaggggg accgctccgc ccgtcgcgcc ccagacgacg
     3661 aacgggacga gctcgccgac gttcgagcag aagtggtagc ccgcgaaccg gcggatgttc
     3721 tcgtacaccg cgcggccctc ctcgacggcc gcgacgaccg aggcgaagtt gtcgtcgagg
     3781 agcaccatgt cggccgcctc ccgcgcgacg tcggtcccgc ggaggcccat cgcgacgccg
     3841 atgtcggcct cccggagggc cggcgcgtcg ttcacgccgt cacccgtcat cgcgacgatc
     3901 tcgccctcct cccgcaggac gtgcgcgagg cgaagcttcg cctccgggtc gatgcgcgcg
     3961 aagacgacct cacgctcggc gaggcgggtg cggagctcct cgtcgtcgag ccgcgccagc
     4021 tccccgccct cgacgacgtg cggctcggat gcgagaccga tctggcgcgc gacggccgcc
     4081 gcggtcgcgc cgctgtcgcc ggtcaccatg acgatgcgga tcccggcccg gcggcagcgc
     4141 gcgaccgcat ccgcgacctc cggccgcggc ggatcgtcca tccccacgag cccgaggagc
     4201 tcgaggccgc gctcgagctc ctcgggcgtg tcgccgccgt ccgcggggac ggtccgccgt
     4261 gcgaccgcga gaacgcgcag cgcgtcgtgc tcgaagcgct ccgcgacggc gagggcctcg
     4321 gcgcgctcga cgggcccgag cgtggagcgg gcgacgatct cctcgggcgc gcccttcgcg
     4381 tacgcgaccc ggccggcgcc gtcgccgtcc aggtgaaggg tggtcatccg cttccgccgg
     4441 gagtcgaacg ggagctccgc gaggcgaggc cggtgcgccg cctcctgctc gtgcctcagg
     4501 ccgcccttct ccgcgaggac gacgagcgcg ccctcggtcg ggtcgccctc gacgcgccag
     4561 ccgtccccgc cgtgcacgag ccgcgcgtcg ttgcagagca gtccggcgcg caggagctcc
     4621 gcgagagtgc gcgggtcggc gacctcaccg tcacgcagga agcgcccgaa cggctcgtag
     4681 ccggcgccct ccacctcgag ctcgccctcc ggcgtccaca cgcgccggac ggtcatctgg
     4741 ttctcggtga gcgtcccggt cttgtccgtg cagatcaccg tcgtcgcgcc gagcgcctcg
     4801 acggaggaga gccgccggac gagggcgttg cgccgcgcca tccgctgcgt ggcgagcgcg
     4861 agcgagagcg tcacggtcgg gaggagcccc tccggcacga gcgcgacggt cacgccgatc
     4921 gcgaagacga agcggtcggc gagcggcatc ccgagcgccc ccgcgacgag aaagaacgtc
     4981 gtcccgatcg cgaccgcgag gacggcgacg aagcgtgtga ggcggtcgag ctggcgctcg
     5041 agcgggctgc ggccctcgcg cgtggcctgc gtgagctcgg cgatccggcc gaagcgcgtc
     5101 cgcatccccg tcgccgtcac gagcgcctcg ccggagccgg ctacgacgta cgtcccggca
     5161 gacacctccg cctccggctc gaccggatac gactcgcccg tcagggccga ctcgtcgacc
     5221 cgcaggccca cgtggacgac gagcgccgcg tccgcgctga ccctgtcgcc ggcggcgagg
     5281 acgagcaggt cgcccgggac gacctcctcc gcggcgatct cgcggacgcc gccgtcgcgc
     5341 cgcacacgcg cgtggggcgg gagcatcgag cggagcgcgg ccaccgcgcg ctcggcccgg
     5401 tactcctgcg cgaacgcgaa gacggcgttc acgagcacga ccgccgcgat cgcgacggag
     5461 agctccggca tcccggcgag ccacgcgagg gccgaccctg tccagaggag cagggcgaac
     5521 aggtgcacga gctgcgccgc gaaccggcgc gcgtgcgagg gccggcgcgg ctcggcgagc
     5581 gcgttcggcc cggcgtccgc gaaccggagg cgcgcctcct cagacgtgag cccggccggc
     5641 ggggcccgca cggcctggcc gagggccatc gtgcctcctt tcttcactcg gaggatcggc
     5701 ccggacgggg cgcggcgcat cgtggaatgc gcgtcgccgc gtgcgggttt ctccctacgc
     5761 ggcggctacg gcacggtgac gagcgcgctg ccctcgagga ggttcgcgcc ggcgtcggcg
     5821 cgcgcccggt acgtgccgct cgaggagatg cgaagcgaga agctgccgtc ggccagcgtg
     5881 cgcgtcgtcc cgacgcgcgc gaacgtcccg tcgggccgct gccggccgat cgtgacgagg
     5941 atgccggcgg ccggggggcg cacgatgccg cgcaggcgcg tgaacggcgc gaccggcttc
     6001 gcgaacgcga cggacgtccg cacctggatc cgctgggcct cgccgttcgc gagcgccgag
     6061 acgacccggt agtcgttcgc gcgcttgggc ttgacgacgc gctcccaggc cccgttcggg
     6121 agcggctcgc cgacggtctc ccacgcggtc tcgccgacgc ggcgccgctc gagcgtggcg
     6181 gtgccccagc cggccgtgcc cccgcgccgc gcgaggccgg agagcttcac ggagccgccg
     6241 tacgtgatcg tcgtggcggc cggcttcagc gagagcacgc cgacgtagaa ccaggtcgag
     6301 cgcaggccga gctccgtgcg cgcagtcgtc ccggagaacg acgcggtcgt cgaggatccg
     6361 cgcgcgacga gggtcgccac gcgggtcgag ctgttccgcg ccgtcacctc gaggtcgacc
     6421 ggccgctgcg cgaggccgag gagcgtctgc accttcgcgg cgccgaagac gcagtcgggc
     6481 gacgtgccgg cgcagtcgat ctcggcgtcc ttcgggcccc aggcatggtg cggcgagatc
     6541 gagtccgggg tgtcgaggac ggagacgagg tacggcaccg gcgccccgcc ccactcgtcc
     6601 gccttcgcgg ccgtccgccc gccggaggtc gagaagaagt acgccgcgat cggcgagcca
     6661 ccgtgggtca tgatctcgcc cgccgtcgac tggacggccg tgttcgtcgc gtccgtctcg
     6721 gccgagcggc cgccgtagac ctggtcgcgc gtgtcagggc agtacacggg gctgccgagc
     6781 cacgtgcagt ggccgccgcc gagggagtac gtccgcgcgg cgaccgcctg cgcggcgagc
     6841 gcctgcgggt gccagctcgc cggcatctcc cgcgggacga cgccctggac gtacggctgg
     6901 cggttcagca cgttcagcgc cgacaccttc ccgctcgccg acgcgaagac gaggctcccg
     6961 cggtacagga cgccgccgag cccgagcgag gccgctcccg gcgcgacgga tgccgggctc
     7021 gcgacggtcg tggtcgcccc gctgcgcgtg aaccgcaccg tcccggttcc cgccgtcacc
     7081 gcgtacgtcc cggcggcaag ggttgtcccg cccgcggtga acgacgatgc cgacgagagc
     7141 gagagcgagg actgcgcgga ggcgagcagg acgcgcacct ggtcgttcgg ctgcccggac
     7201 gtcaacgacg cgcccgtgta gtaatgctcg aggatcgcct cgtagcccca gccctggagc
     7261 gcgcggccct gcgtcccgta ctggccgagc ccgatcccgt gcccccagcc cttccccgtc
     7321 aggacgaaga cgggcgcgcc gtgcgccgcc ggcgcgacga gggccgcgag gaggacgagc
     7381 agaacgacga ggacacgcct gtgcatacac aacagagtcg cacgagaacg cgtcatccgc
     7441 atcggtgtcc tccccgatcg cgggacggcg ccgcgcgggc tctctgctac ggtggaagca
     7501 cggacggcag cccgtcgtcc cgtgagatcg cttctcgttc ctcttcggcg gcttcgagcg
     7561 ggtgatctcg agccgacaac cgaaggagta gaacgtgtcc caggagtttc gcgcgctcgg
     7621 cgtgagcgcg gacgtcgtag aggtgctcgc ctcgcgcgac atcgtgtccc ccttttccat
     7681 ccagaccgcg gtcctgcccg aggcgctctc gggcggcgac gtgctggcga agtccccgac
     7741 cggctcgggc aagacgctcg cgttcggcct gccgatcgtc gagcggctgc gtccggacga
     7801 ggggacgccg gccgcgctcg tgctcgtccc cacgcgggag ctggccgccc aggtggccga
     7861 gcagctcgag ccgatcggcg cggtgaaggg gctcagggtc gcgaccgcgt acggcggcgc
     7921 gccgatgggc ccgcaggtga agcgcctgaa gggcgcgcac atcgtcgtcg cgacgcccgg
     7981 ccggctgcag gacctcgtcg agcggcgcca cgtcgtcctc tcgaaggtcc gcgtgctcgt
     8041 cctcgacgag gccgaccgga tgctcgacat gggcttcaag ccccaggtcg accggctcgt
     8101 tcgccggctc ccccgcgagc g
//
LOCUS       DASTFC010000063        12391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_396713,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000063 DASTFC010000000
VERSION     DASTFC010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396713"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFO88_01835"
     CDS             <1..651
                     /locus_tag="VFO88_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HET9544296.1"
                     /translation="ARAVEHAGGEGDGLELTICARNFELEVDLFVSAGGEERAAAVED
                     ALVVGFGDALFARDERPVAALVLDRCRELGLTLATAESCTGGLVGARLTDVAGASDAY
                     LGGVVAYSNEVKAARLGVGEDVLARHGAVSEETARAMAAGARAALGADVGIAVTGVAG
                     PGGGTAEKPVGLVHVASEGPGGATAREFRFPGDREEIRERAVVMALHEARTLLARL"
     gene            702..1244
                     /gene="thpR"
                     /locus_tag="VFO88_01840"
     CDS             702..1244
                     /gene="thpR"
                     /locus_tag="VFO88_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET9544297.1"
                     /translation="MEGDDRHRLFVALPLPEETVERLARWGAVELAGARDARLVPPEN
                     LHVTLAFLGSRPADELGAIAAELAGAARAAEPPVLTVERYRETRSVGMVALGDEGGRA
                     TRLAADVAERLARIGVYRPEERPWLPHVTVLRFRRRPRLAPAPPDLGRVSPSDVALYT
                     SVLRRTGAQYAIRDAFPLGG"
     gene            1252..2379
                     /gene="recA"
                     /locus_tag="VFO88_01845"
     CDS             1252..2379
                     /gene="recA"
                     /locus_tag="VFO88_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9544298.1"
                     /translation="MDREQALDAALSQIERAYGKGSVMKMSDQAQVAIPSISTGSLSL
                     DVALGIGGLPRGRVIEVFGPESSGKTTLVYHVIAEAQRRGGICAFVDAEHAMDPAYAR
                     RIGVNIDDLLVSQPDNGEQALEIAELLIRSGALDVVAVDSVAALTPRAEIEGEMGDSH
                     VGLQARLMSQALRKLAGTLNKTGTVALFTNQLREKIGVMFGNPETTPGGRALKFYSSV
                     RLDIRRIETLKEGVEAIGNRVRVKVVKNKVAPPFKQAEFDIIYGEGISWEGSVLDVGL
                     DRKIVQKSGSYFSFEDERLGQGRQNATAFLREHPDVTQGILRAIQLQLGPDAVVSSRL
                     LPTTEGEAPAEAAPVQEAAANGDGDPAEAAEAAPRAKASAR"
     gene            2381..2959
                     /locus_tag="VFO88_01850"
     CDS             2381..2959
                     /locus_tag="VFO88_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET9544299.1"
                     /translation="MPRLTALRRARPGLVALELDGRPWRTVPDDAVARAGIAAGDELD
                     RPALRRLRAELVRARGLRVATRALARRDLSAAELDARLERARVAPTVAADVADTLGRA
                     GLVDDERFAAGRAAQLAARGWGDAAIAARLEGAGVDEAVARAAIAALEPEADRAEALL
                     RGVDDPFRRARLLARKGFGEDTLERLAGAALD"
     gene            3202..4737
                     /gene="rny"
                     /locus_tag="VFO88_01855"
     CDS             3202..4737
                     /gene="rny"
                     /locus_tag="VFO88_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET9544300.1"
                     /translation="MQLVIGIVLGVAAGLAVGALVASRVAIGRRRAAAEQEIARMTAD
                     AERSADAIRREAQVEAKEEALKLRAEVERELADRRAETIKVEERTAAREQDVERTLTE
                     LERREQGLADRELHVKELQESLKETRDAQVRELERIAGLTTADAKRQLLEQAEDQIRR
                     DMGRMVRAAEEEAQAEAKKRARNLVADALQRVAASHAAETTVSLVELPSDDMKGRIIG
                     REGRNIRALENLTGVDFIIDDTPQAVVLSSFDGVRREVAKLTLAKLVEDGRIHPSRIE
                     EMYYQSKAEIEDHIRQLGEQAVFEANVGTMHDELIKLLGRLRFRTSYGQNILKHSLEC
                     THLAGIMASELGASVKTAKRAALLHDAGKAMTHEVEGSHASISAQMAKRYGESQHVVH
                     AIEAHHYEVQPQTVEAVLVIAADAISASRPGARGESLEHYVKRLENLEGLASAKKGVE
                     RVFAIQAGREIRVMVKPAEVDDDEAALLSHEIAREIEGQLEYPGQIKVTVIRESRATE
                     YAK"
     gene            complement(4777..9165)
                     /locus_tag="VFO88_01860"
     CDS             complement(4777..9165)
                     /locus_tag="VFO88_01860"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HET9544301.1"
                     /translation="MRLTVARRRLSVLALLAFVAALAAQPAAADTTAQPLPFTQNWTN
                     IDLITALNDWSGVPGIIGYRGDGMTSATGVDPQTVLADGSGTLVNVLANQTTPNTIAT
                     GGVAEFHLENPVVALQGSGTARAPHLVLTLDTTGFATVTVGYLLRDVDASIDNSVQPI
                     ALQYRVGSSGSFTNVPSAFVADASAGPSATLETPVTAVLPAAAGNQSLVQVRVITTDA
                     VGSDEWIGIDDISVTGSNDPGDTAPAVQSTSPVDGATGVAGNANITITFNEAVTVAAG
                     GFGVTCGIVGSQPVTVSGGPTTFTLSPLSPLPAASTCTVTVTATAVSDNDTIDPPDQM
                     AADHVFSFMTAAPGPAPGSVVVSEVYGGGGNSGATLTHDFIELYNRTGAAVSLSGWSV
                     QYASSAGSTWQVTPLAGSIPSGRNYLVQEAAGAGGTTPLPTPDATGSIAMSATAGKVA
                     LVASTTPLSGTCPTGGAIVDFVGYGSAANCFEGTGPTGTLSNTTSAQRKDGGNQDTDN
                     NSEDFTVGAPDPRSSGEPAPAVSASTPAAGAVGVRRGANIQLVFTEPVDVAGAWFTIN
                     CTTSGPHTATASGGPVTFTLDPDGDFAFDETCTVTILAANVTDQDADDPPDNMAADHV
                     FSFQTEELLTCGDPATGIHAVQGSGTSTPLAGQRVTIEGVVVGDYQLQPSEFGGFYLQ
                     EEDAHADGDPATSEGIFVFDNGFGVDVAPGDVVRVRGTAGESFSLTQIAGITGFSVCG
                     SGASVTAASVSLPVANVPDHERTEGMLVNFAQLLTATEVFNLGRFGEVSLSGVGRLWN
                     PTAVALPGAPALDVLAQNNRSRIILDDGNNQQNIDPTRYPQGGLSASNTLRVGDTLDG
                     LTGVMDFRFSNYRIQPVGPIEFDVGNPRTPAPEPVGGNLKVASFNVLNYFNGDGLGGG
                     FPTSRGALSPFELERQSAKIVSALAAIDADVVGLMEIENDGGPNSALQELVRRLNDEV
                     GAGTYAAIDTGVIGTDEIKVALIYKPAVVSPVGDWKILTSAIDPRFDTSLNRPALAQT
                     FEHAASDERLTVAVNHLKSKGTPCPADPDMGDGQGNCANVRNLAAAALADWLATDPTG
                     SGDPDRLVIGDLNAYTFEDAIRTLEVKGYVNLVRQFNGLTAYSYVFQGESGYLDHALA
                     NASLAAQVTGTTEWHINPDEPNVLDYTTSFKTANQIVTFYDPGPYRASDHDPVIVGLS
                     MNLAPEVDAGGPYSVDEGGTVGLSATGSDPDGEDLTYAWDLDGDGTYETPGQTATFAA
                     GTLDGPGSVTVGVRVTDERGKTATDTATIQIVNVAPSGTFEAPASAPAGFAFELALSG
                     VTDPSAADTAAGFEYAFDCGSGYGAFGAASSASCPTDAVETRSVGVQVRDKDGGVREY
                     SATVDVFVTFDSLCDLVQALSSDPDVADSLCERLAHAENAPNAGVRKRLLDAFRNQVR
                     AQSGEQPDKAFTPAEGALIEALSLEL"
     gene            9280..10608
                     /gene="miaB"
                     /locus_tag="VFO88_01865"
     CDS             9280..10608
                     /gene="miaB"
                     /locus_tag="VFO88_01865"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET9544302.1"
                     /translation="MRRYHVTTFGCQMNAHDSERIKGMLESLGLGEAVSQAEADVIVF
                     NTCTIREKPDQRFAAHLAQARALKERDPGTVIAVGGCYAEAQRERLFELYPEVDVAFG
                     PGSIPHLGEWLGAGGVGVGRGRFGIAEERAFAGELPMHRERSFQAWVQVSMGCNSRCS
                     YCIVPSVRGREVSRRPGEIVAEVTRLADEGVREVTLLGQNVNSWGRDLAPDVRTEFGE
                     LLRACDAVEGIERIRFTSPHPKDFRDPVVAAMAECDAVCEHIHLPLQSGSDRILKAMR
                     RTYDRARYVRLVEKLRAAIPDLALGTDIIVGFPGETEDDFRATLEAVEEVRYDSAFTF
                     IYSPRRGTEAAGLDAQVPYEVKRDRLERLVDVVQRIAAERNAERVGGVEEVLVEGPSR
                     TDEARLRGRTRRNTTVNFDGSARPGELVPVEITSATSTTLGGRRTALVAA"
     gene            10706..11626
                     /gene="miaA"
                     /locus_tag="VFO88_01870"
     CDS             10706..11626
                     /gene="miaA"
                     /locus_tag="VFO88_01870"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET9544303.1"
                     /translation="MREGPDGFVLAIFGPTASGKTAVAEALAERIPAEIVSADALQVY
                     DGLPILTNQSERPARLVAIWPLDHEASVGEYERLAHAAIDEVLALGRTPIVAGGTGLY
                     LRAALTNLRLPPPPPPGARERLEARYDELGPERAHAELADRDPAAAAAVHPNDRRRVV
                     RALELAEAGASLAPATDRLWSEETRRPTVLVGLDVPPEELERRIEARTAAMFERGVEE
                     EVARALARPLSRTARAVLGLEEIASLPRDDAEREIVVRTRRYAAYQRKWMRRIPGLVT
                     VRADRPADEVADAILEVARARQRVPARRAG"
     gene            11568..12350
                     /gene="dapF"
                     /locus_tag="VFO88_01875"
     CDS             11568..12350
                     /gene="dapF"
                     /locus_tag="VFO88_01875"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET9544304.1"
                     /translation="MRFSKWHALGNAYLLVERADGAPLDAGLARRLCDPSTGIGSDGV
                     LEVVADGAAADVTVWNPDGSQAEFSGNGARIAAAWLAARAGSAEVVLRFGAREVVASV
                     RGSDVEVDAGPVEVGPPEELALEIERLEFTPVSVGNPHAVVRREFGDLDVVRLGPLVE
                     CHTRVPGRTTVLLVRVTGPHELEIGIWERGVGVTRSSGSSSVAAAAAAVANGWVESPV
                     TVKVPGGGALTVTLAGGRARLTGPVEEICRGEVVLGEQSAPR"
BASE COUNT         1668 a         4363 c         4709 g         1651 t
ORIGIN      
        1 gcgcgggcgg tcgagcacgc gggtggggag ggggacgggc tcgagctcac gatctgcgcg
       61 cgcaacttcg agctcgaggt cgacctcttc gtgtcggcgg gcggggagga gcgagcggcg
      121 gccgtcgagg acgcgctcgt ggtcgggttc ggagacgcgc tcttcgcccg cgacgagcgg
      181 ccggtcgcgg cgctcgtcct cgaccggtgc cgagagctcg ggctgacgct cgcgaccgcg
      241 gagagctgca cgggcgggct cgtgggcgcg cggttgaccg acgtcgcggg cgcgagcgac
      301 gcgtacctcg gcggcgtggt cgcatactcg aacgaggtga aggcggcccg gctcggcgtg
      361 ggggaggacg tgctcgcgcg ccacggggcg gtgtcggagg agaccgcgcg tgcgatggcc
      421 gccggggcgc gggcggcgct cggcgccgac gtcggcatcg cggtcaccgg cgtcgcgggc
      481 ccggggggcg gtacggcgga gaagcccgtc gggctcgtcc acgttgcctc cgaggggccg
      541 ggaggggcca cggcgcgcga gttccggttc ccgggcgacc gggaggagat ccgcgagcgg
      601 gcggtcgtga tggccctcca cgaggcgcgc acgcttctgg cacggctctg acacggaatc
      661 ggcacatccc ggcgcactcc cgtgcggatt cgccgcctag ggtggagggc gatgaccgcc
      721 accgcctctt cgtcgcgctc ccgctcccgg aggagaccgt ggagcgactc gcccgctggg
      781 gcgccgtgga gctcgccggc gcacgggacg cccggctcgt cccgccggag aacctccatg
      841 tcacgctcgc gttcctcggc tcgcggcccg cggacgagct gggcgcgatc gccgcggagc
      901 tcgccggggc cgcccgcgcg gccgagccgc cggtgctcac cgtggagcgc tatcgcgaga
      961 cgcggagcgt cggcatggtc gccctcggcg acgagggcgg acgggcgacg cggctcgccg
     1021 cggacgtggc ggagcggctc gcgcggatcg gcgtctaccg gccggaggag cggccgtggc
     1081 tcccgcacgt gaccgtcctg cgcttccgcc ggcggccgcg cctcgcgccc gcgccgcccg
     1141 acctcgggcg ggtcagtccg tccgatgtcg ctctctacac ttcagtcctg cgccggacgg
     1201 gggcgcagta cgcgatccgg gacgcattcc cgctaggagg ctgagacacc gatggaccgt
     1261 gagcaagcac tcgacgcagc cctgagccag atcgagcggg cctacggcaa gggctccgtg
     1321 atgaagatga gcgaccaggc ccaggtcgcg atcccctcga tctcgaccgg gtcgctgtcg
     1381 ctcgacgtgg cgctcgggat cggcggcctg ccgcgcggcc gcgtgatcga ggtcttcggc
     1441 cccgagagct cggggaagac gacgctcgtc taccacgtga tcgccgaggc gcagcgccgc
     1501 ggcgggatct gcgcgttcgt ggacgccgag cacgcgatgg acccggcgta cgcgcgccgc
     1561 atcggcgtga acatcgacga cctcctcgtc tcgcagcccg acaacgggga gcaggcgctc
     1621 gagatcgccg agctcctcat ccgctcgggc gcgctcgacg tcgtcgccgt cgactcggtc
     1681 gccgcgctca ccccgcgcgc cgagatcgag ggcgagatgg gggacagcca cgtcggcctc
     1741 caggcgcggc tcatgagcca ggcgctccgc aagctcgccg gcacgctgaa caagacgggc
     1801 accgtcgcgc tcttcacgaa ccagctccgc gagaagatcg gcgtcatgtt cgggaacccc
     1861 gagacgacgc ccggcgggcg cgcgctcaag ttctactcct cggtgcggct cgacatccgg
     1921 cgcatcgaga cgctgaagga gggcgtcgag gcgatcggga accgcgtgcg cgtgaaggtc
     1981 gtgaagaaca aggtcgcgcc gccgttcaag caggcggagt tcgacatcat ctacggcgag
     2041 gggatctcct gggagggctc cgttctcgac gtcgggcttg accggaagat cgtccagaag
     2101 tccggctcgt acttctcctt cgaggacgag cggctcgggc aggggcgcca gaacgcgacc
     2161 gcgttccttc gcgagcaccc ggacgtgacg caggggatcc tgcgggcgat ccagctccag
     2221 ctcgggccgg acgcggtcgt gtcctcgcgg ctcctcccga cgacggaggg cgaggctccc
     2281 gccgaggcgg cgcccgtgca ggaggcggcg gcgaacggcg acggcgaccc ggcggaggcc
     2341 gccgaggccg cgccgcgggc gaaggcgtcc gcgcgctagc gtgccgcgcc tgacggcgct
     2401 gcggcgcgcg cgcccgggcc tcgtcgcgct cgagctcgac gggcgcccgt ggaggacggt
     2461 tcccgacgac gcggttgcgc gggcaggcat cgccgcgggc gatgagctcg accgccccgc
     2521 gctccgccgg ctgcgggccg agctcgtgcg cgcccgcggc ctgcgggtcg cgacgcgggc
     2581 gctcgcgcgg cgcgacctct cggcggccga gctcgacgcc cggctcgagc gcgcccgcgt
     2641 cgcgcccacc gtcgccgcgg acgtcgccga caccctcgga cgggcggggc tcgtcgacga
     2701 cgagcggttc gccgcggggc gcgcggcgca gctggcggcc cgcggctggg gcgacgctgc
     2761 gatcgcggcg cgcctcgagg gcgcgggcgt ggacgaggcc gtcgcccgcg cggcgatcgc
     2821 cgccctggag ccggaggcgg accgcgcgga ggcgctcctc cgcggcgtcg acgacccgtt
     2881 ccgccgcgcg cggctcctcg ctcggaaggg cttcggtgag gacacgctcg agcgcctcgc
     2941 gggcgccgcg ctggactgac cggcctcgcc ggggctagga tacggtttcc atcacgacaa
     3001 aaacgcctgc aacggaacgg ctttacgccg aaatcggact cagcgacacg accacgactc
     3061 cacgaaccac tgacatgacc gaacgagcga gcagacggag gggaccgacc gccgaccggt
     3121 agtcgcgcgc ctgcgcacgc cgtccgcccg caccgattcc gacgaccggc cgttcctacg
     3181 ggctcaacgt aggagcgagc aatgcagctt gtgatcggca tcgtcctcgg cgtcgccgcc
     3241 gggctcgccg tgggcgcact cgtggcctcc cgggtcgcga tcgggcgccg ccgcgcggcc
     3301 gccgagcagg agatcgcacg catgacggcc gacgccgagc ggagcgcgga cgcgatccgc
     3361 cgcgaggcgc aggtcgaggc gaaggaggag gcactgaagc tccgcgccga ggtcgagcgg
     3421 gagctcgccg accggcgggc ggagacgatc aaggtcgagg agcgcaccgc ggcgcgcgag
     3481 caggacgtcg agcgcacgct gacggagctc gagcgccgcg agcagggcct cgcggaccgc
     3541 gagctccacg tgaaggagct ccaggagtcc ctcaaggaga cgagggacgc gcaggtccgc
     3601 gagctcgagc gcatcgcggg cctcacgacc gcggacgcga agcggcagct cctcgagcag
     3661 gccgaggacc agatccggcg cgacatgggc cggatggtca gggcggccga ggaggaggcg
     3721 caggcggagg cgaagaagcg cgcccgcaac ctcgtcgcgg acgcgctcca gcgcgtcgcg
     3781 gcgagccacg ccgccgagac gacggtctcg ctcgtcgagc tcccctcgga cgacatgaag
     3841 ggacggatca tcggccgcga gggccgcaac atccgcgcgc tcgagaacct caccggcgtc
     3901 gacttcatca tcgacgacac gccgcaggcg gttgtcctct cgtcgttcga cggcgtccgg
     3961 cgcgaggtcg cgaagctcac gctcgcgaag ctggtcgagg acgggcgcat ccacccgtcg
     4021 cggatcgagg agatgtacta ccagtcgaag gcggagatcg aagaccacat ccgccagctc
     4081 ggcgagcagg ccgtcttcga ggcgaacgtc ggcacgatgc acgacgagct gatcaagctc
     4141 ctcgggcgcc tccgcttccg gacgagctac gggcagaaca tcctcaagca ctcgctcgag
     4201 tgcacccacc tcgccgggat catggcctcc gagctcggcg cgagcgtgaa gacggccaag
     4261 cgcgccgcgc tcctccacga cgccggcaag gcgatgacgc acgaggtcga gggctcgcac
     4321 gcctcgatct ccgcccagat ggcgaagcgc tacggcgagt cgcagcacgt cgtccacgcg
     4381 atcgaggcgc accactacga ggtgcagccg cagacggtcg aggcggtgct cgtgatcgcc
     4441 gccgacgcta tctccgcctc acgccccggc gcgcgcggcg agagcctcga gcactacgtg
     4501 aagcgcctcg agaacctcga gggccttgcg tccgcgaaga agggcgtcga gcgcgtgttc
     4561 gccatccagg cgggccgcga gatccgtgtg atggtgaagc cggccgaggt ggacgacgac
     4621 gaggcggccc tcctctcgca cgagatcgcg cgcgagatcg agggccagct cgagtacccc
     4681 ggccagatca aggtcacggt catccgcgag agccgcgcca ccgagtacgc gaagtagggc
     4741 ggaggggccg ggtcgcgccc ggcccctccg ctaccgctag agctcaaggg agagcgcctc
     4801 gatcagcgcg ccctcggcgg gcgtgaacgc cttgtccggc tgctcgccgc tctgggcgcg
     4861 aacctggttt cggaacgcgt ccaggaggcg cttgcggacg ccggcgttcg gcgcgttctc
     4921 cgcgtgcgcg agccgctcgc agagcgagtc ggccacgtcc gggtcgctcg agagcgcctg
     4981 gacgaggtcg cagaggctgt cgaacgtgac gaagacgtcg acggtcgcgc tgtactcgcg
     5041 gacgccgccg tccttgtcgc gcacctggac gccgaccgac cgcgtctcga cggcgtccgt
     5101 cgggcaggag gccgacgacg cggcgccgaa cgccccgtag cccgagccgc agtcgaacgc
     5161 gtactcgaag ccggcggccg tgtcggccgc ggacgggtcg gtcaccccgc tcagcgcgag
     5221 ctcgaacgcg aacccggcgg gcgccgaggc gggcgcctcg aacgtgccgc tcggagcgac
     5281 gttcacgatc tggatcgtcg ccgtgtcggt cgcggtcttt ccgcgctcgt cggtcacccg
     5341 gacgccgacg gtgacgctgc ccggcccgtc gagcgtcccg gccgcgaacg tcgccgtctg
     5401 gcccggcgtc tcgtacgtcc cgtcgccgtc gaggtcccac gcgtacgtca ggtcctcgcc
     5461 gtccgggtcg ctcccggtcg cggagagccc gacggtgccg ccctcgtcga ccgagtaggg
     5521 cccgcccgcg tccacctcgg gcgcgaggtt catgctgagc ccgacgatca ccgggtcgtg
     5581 gtcggacgcc cggtaggggc cggggtcgta gaacgtgacg atctggttcg ccgtcttgaa
     5641 cgacgtcgtg tagtcgagga cgttcggctc gtccgggttg atgtgccact cggtcgtgcc
     5701 cgtcacctgc gccgcgagcg acgcgttcgc gagggcgtgg tcgaggtagc cggactcgcc
     5761 ctggaagacg taggagtacg ccgtcaggcc gttgaactga cggacgaggt tgacgtagcc
     5821 cttcacctcg agcgtccgga tcgcgtcctc gaaggtgtac gcgttcaggt ctccgatgac
     5881 gagccggtcg gggtcgccgc ttccggtcgg gtcggtcgcg agccagtccg cgagcgccgc
     5941 cgcggccagg ttgcggacgt tggcgcagtt gccctggccg tcgcccatgt ccgggtcggc
     6001 cgggcacggc gtccccttcg acttgaggtg gttcaccgcg accgtcagcc gctcgtccga
     6061 cgccgcgtgc tcgaacgtct gcgcgagcgc gggccggttg agcgacgtgt cgaaccgcgg
     6121 gtcgatcgcc gacgtgagga tcttccagtc accgaccggc gagacgacgg ccggcttgta
     6181 gatgagcgcg accttgatct cgtccgtgcc gatcacgccg gtgtcgatcg ccgcgtacgt
     6241 gccggccccg acctcgtcgt tcagccgccg gacgagctcc tggagcgcgc tgttcggccc
     6301 gccgtcgttc tcgatctcca tgaggccgac gacgtccgcg tcgatcgcgg cgagggcgct
     6361 cacgatcttc gccgactgcc gctcgagctc gaacgggctg agcgcgccgc gggacgtcgg
     6421 gaagccgccg cccaggccgt ccccgttgaa gtagttgagg acgttgaagg acgcgacctt
     6481 caggttcccg ccgaccggct cgggagccgg cgtccgcggg ttcccgacgt cgaactcgat
     6541 cgggccgacc ggctggatcc ggtagttcga gaaccggaag tccatgacgc ccgtcagccc
     6601 gtccagcgtg tcgccgacgc gcagcgtgtt cgacgcgctg agcccgccct gcgggtagcg
     6661 cgtcgggtcg atgttctgct ggttgttgcc gtcgtcgagg atgatccggc tgcggttgtt
     6721 ctgcgccagg acgtcgagcg ccggggcgcc gggcagcgcg accgccgtcg ggttccagag
     6781 ccgtcccacg ccggagaggc tgacctcgcc gaagcggccg aggttgaaga cctcggtcgc
     6841 cgtcaggagc tgggcgaagt tgacgagcat gccctccgtc cgctcgtggt cgggcacgtt
     6901 cgcgaccggg agggagacgc tcgccgcggt gacgctcgcg ccgctcccgc agacggagaa
     6961 gcccgtgatg cccgcgatct gcgtcaggct gaacgactcg ccggccgtcc cgcgcacccg
     7021 cacgacgtcc cccggggcga cgtcgacgcc gaacccgttg tcgaagacga agatgccctc
     7081 ggacgtggcc gggtcgccgt cggcgtgcgc gtcctcctcc tggaggtaga agccgccgaa
     7141 ctcggacggc tggagctggt agtcgccgac gacgacgccc tcgatcgtca cgcgctggcc
     7201 ggcgagcggc gtcgaggtgc cggagccctg cactgcgtgg atcccggtcg ccgggtcgcc
     7261 gcacgtgagc agctcctcgg tctggaagga gaagacgtgg tccgccgcca tgttgtcggg
     7321 cgggtcgtcc gcgtcctggt cggtcacgtt cgcggcgagg atcgtcacgg tgcacgtctc
     7381 gtcgaaggcg aagtcgccgt ccgggtcgag cgtgaacgtc acgggcccgc ccgacgcggt
     7441 cgccgtgtgc gggccgctcg tcgtgcagtt gatcgtgaac cacgcgccgg cgacgtccac
     7501 gggctcggtg aagacgagct ggatgttcgc gccgcgccgg actccgacgg cgcccgccgc
     7561 gggcgtcgac gccgagacgg ccggggccgg ctcgccgctc gagcgcgggt cgggcgcgcc
     7621 gaccgtgaag tcctccgagt tgttgtccgt gtcctggttg cccccgtcct tccgctgtgc
     7681 ggaggtcgtg ttcgagagcg ttccggtcgg gccggtgccc tcgaagcagt tcgccgccga
     7741 gccgtagccg acgaagtcga cgattgcgcc gccggtcggg cacgtgccgc tgagcggcgt
     7801 cgtggaggcg acgagtgcca ccttccctgc ggtggcggac atggcgatcg agccggtcgc
     7861 gtcgggggtc gggagcggcg tcgtcccgcc ggcgccggcc gcctcctgga cgaggtagtt
     7921 gcgcccggac gggatggagc cggcgagcgg ggtcacctgc cacgtcgagc ccgccgagga
     7981 cgcgtactgg acggaccagc cggagaggct cacggccgct ccggtgcggt tgtagagctc
     8041 gatgaagtcg tgggtgagcg tggcgcccga gttgccgccg ccgccgtaca cctcgctgac
     8101 gacgacgctt ccgggcgcgg ggcccggcgc ggcggtcatg aaggagaaga cgtggtcggc
     8161 cgccatctga tcgggcgggt cgatcgtgtc gttgtcgctc acggccgtgg ccgtgacggt
     8221 gaccgtgcag gtcgaggccg ccgggagcgg cgagagcggg gagagcgtga aggtcgtcgg
     8281 gccgccgctc accgtgaccg gctgggagcc gacgatgccg cacgtgacgc cgaagccgcc
     8341 ggcggcgacg gtcacggcct cgttgaacgt gatcgtgatg ttcgcgttgc cggcgacgcc
     8401 cgtcgcgccg tcgaccgggc tcgtcgactg aacggccggg gcggtgtcgc ccgggtcgtt
     8461 cgagccggtg accgagatgt cgtcgatccc gatccactcg tccgagccga ccgcgtccgt
     8521 ggtgatgacg cgcacctgga cgagcgactg gttccccgcc gccgcgggga gcaccgcggt
     8581 gacgggcgtc tcgagcgtcg cgctcgggcc cgcggacgcg tcggcgacga acgcggacgg
     8641 gacgttcgtg aaggatccgc tcgagccgac gcggtactgg agcgcgatcg gctggacgct
     8701 gttgtcgatg ctcgcgtcga cgtcacgcag gaggtacccg accgtcacgg tcgcgaagcc
     8761 ggtcgtgtcg agcgtgagga cgaggtgcgg cgcgcgcgcg gtgccggagc cctgcagcgc
     8821 gacgaccggg ttctcgaggt ggaactcggc cacgccgccg gtcgcgatcg tgttgggggt
     8881 cgtctggttc gcgagcacgt tgacgagcgt tccggacccg tcggcgagga cggtctgcgg
     8941 atcgacaccg gtagcgctcg tcatgccgtc gccccggtag ccgatgatcc cgggcacgcc
     9001 ggaccagtcg ttcagggcgg tgatcagatc gatgttcgtc cagttctgcg tgaacggaag
     9061 cggctgcgcc gtcgtgtcgg cggcagcggg ctgcgcggcc agcgccgcca cgaacgcaag
     9121 aagcgccagg acggacagcc gccggcgcgc cacagtaagt ctcatgtctc tcccctgtaa
     9181 ggacaggtcc cccgcggacc agtgcgcgag ggagggtagc accgtttcgg acgaagtccg
     9241 aatggccgac gagcgcgccg gggctaccat tcgcgggcga tgcgccgcta ccacgtcacg
     9301 acgttcggct gccagatgaa cgcgcacgac tccgagcgga tcaagggcat gctcgagtcg
     9361 ctcgggctcg gcgaggcggt ctcccaggcg gaggccgacg tgatcgtctt caacacgtgc
     9421 acgatccgcg agaagcccga ccagcgcttc gccgcgcatc tcgcgcaggc ccgggcgctc
     9481 aaggagcgcg acccgggcac ggtgatcgcg gtcggcggct gctacgcgga ggcgcagcgc
     9541 gagcggctct tcgagctcta tccggaggtg gacgtcgcgt tcgggcctgg ctcgatcccg
     9601 catctcggcg agtggctcgg cgcgggcggg gtcggcgtcg gacggggccg cttcgggatc
     9661 gcggaggagc gcgcgttcgc gggcgagctg ccgatgcacc gcgagcgctc gttccaggcg
     9721 tgggtgcagg tgtcgatggg ctgcaactcg aggtgctcgt actgcatcgt cccgtccgtg
     9781 cgcggccgcg aggtcagccg ccggccgggt gagatcgtcg cggaggtgac gcggctcgcg
     9841 gacgagggcg tgcgcgaggt cacgctcctc gggcagaacg tgaactcgtg ggggcgcgac
     9901 ctcgcgcccg acgtgcgcac ggagttcggc gagctcctgc gcgcctgcga cgcggtggag
     9961 gggatcgagc ggatccgctt cacgagcccg cacccgaagg acttccgcga ccctgtcgtc
    10021 gcggcgatgg ccgagtgcga cgcggtctgc gagcacatcc accttccgct ccagtcgggc
    10081 tccgaccgca tcctcaaggc gatgcgccgc acgtacgacc gcgcgcgcta cgtgcggctc
    10141 gtcgagaagc tccgggccgc gatccccgac ctcgcgctcg ggacggacat catcgtcggc
    10201 ttccccggcg agaccgagga cgacttccgg gcgacgctcg aggcggtcga ggaggtgcgc
    10261 tacgacagcg ccttcacgtt catctactcg ccgcggcgcg gcaccgaggc ggccgggctc
    10321 gacgcccagg tgccgtacga ggtgaagcgc gaccggctcg agcggctcgt ggacgtcgtc
    10381 caacggatcg ccgccgagcg gaacgccgag cgcgtcggcg gggtggagga ggtgctcgtg
    10441 gagggcccgt cgcgtacgga cgaggcgcgc ctgcgcggcc gcacgcgccg gaacacgacg
    10501 gtgaacttcg acggctcggc gcgacccggc gagctcgtcc cggtcgagat cacgagcgcg
    10561 acctcgacga cgctcggcgg tcgccggacg gcgctcgtcg ccgcttgacg cgaactcggc
    10621 acattccggc acacatccgc gcgcccttcg gcgatagcat cgaaagcggg tggtgggctg
    10681 gggtgccccg gatccggagc gagtcgtgag ggaagggccg gacggcttcg tgctcgcgat
    10741 cttcggtccg acggcgagcg ggaagacggc ggtcgcggag gcgctggcgg agcggatccc
    10801 ggcggagatc gtctcggccg acgccctcca ggtgtacgac gggctgccga tcctcacgaa
    10861 ccagtccgag cggccggcgc ggctcgtcgc gatctggccg ctcgaccacg aggcgtccgt
    10921 cggggagtac gagcggctcg cgcacgcggc gatcgacgag gtgctcgcgc tcggccgcac
    10981 gccgatcgtc gccggcggca ccgggctcta cctgcgcgcc gcgctcacga acctccgcct
    11041 cccgccgccc ccgccgccgg gcgcgcgcga gcgactcgag gcgcgctacg acgagctggg
    11101 cccggagcgc gcgcacgccg agctcgccga ccgcgacccg gccgccgccg cggccgtcca
    11161 cccgaacgac cggcgccgcg tcgtccgcgc gctcgagctg gccgaggcgg gagcctccct
    11221 cgcgcccgcg accgatcgcc tctggagcga ggagacgcgc cgtccgaccg tgctcgtcgg
    11281 gctcgacgtc ccgccggagg agctcgagcg gcgcatcgag gcccgcacgg cggcgatgtt
    11341 cgagcgcggg gtggaggagg aggtcgcgcg cgcgctcgcc cggccgctct cgcgcaccgc
    11401 acgggccgtt ctcggcctcg aggagatcgc gtcgctcccg cgcgacgacg ccgagcggga
    11461 gatcgtcgtc cgcacgcgcc gctacgcggc ctaccagcgc aagtggatgc ggcggatccc
    11521 gggcctcgtt accgttcggg ccgaccggcc cgcggacgag gtggcggatg cgattctcga
    11581 agtggcacgc gctcggcaac gcgtacctgc tcgtcgagcg ggctgacggc gcgccgctcg
    11641 acgccggcct cgcccgccgg ctgtgcgacc cgagcacggg gatcggctcg gacggcgtcc
    11701 tcgaggtcgt cgcggacggc gcggccgcgg acgtgaccgt ctggaacccg gacggctcgc
    11761 aggcggagtt ctccggcaac ggcgcgcgga tcgcggcggc gtggctcgcg gcgcgcgccg
    11821 gcagcgccga ggtcgtcctc cggttcggcg cgcgcgaggt tgtcgcgagc gttcgcggat
    11881 cggatgtcga ggtcgacgcg ggtcccgtcg aggtcgggcc gccggaggag ctcgcgctcg
    11941 agatcgagcg gctcgagttc acgccggtct ccgtcgggaa cccgcacgcg gtcgtgcggc
    12001 gtgagttcgg cgacctcgac gtcgtccgcc tcggcccgct cgtcgagtgc cacacgcgcg
    12061 tccccggccg gacgaccgtc ctgctcgtcc gcgtgacggg cccgcacgag ctcgagatcg
    12121 gtatctggga gcgcggcgtc ggcgtcaccc ggtcgtccgg ctcgtcctcg gtcgccgcgg
    12181 ccgccgccgc ggtcgcgaac ggctgggtcg agagcccggt caccgtcaag gtgcccgggg
    12241 gcggcgccct caccgtcacg ctcgcaggcg gccgcgcccg cctcaccggc ccggtcgagg
    12301 agatctgccg cggcgaggtc gtcctcggcg agcagagcgc ccctcgctag tgacacagtg
    12361 ttactagcga ggggttcgcc tgtccctcag a
//
LOCUS       DASTFC010000064         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_17560,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000064 DASTFC010000000
VERSION     DASTFC010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17560"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..408)
                     /locus_tag="VFO88_01880"
     CDS             complement(238..408)
                     /locus_tag="VFO88_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544305.1"
                     /translation="MASFEATVVGAIGFAEVDGQRLIAIRQTSPTVGSLDVVECTASG
                     IGTVYLSFVRRA"
     gene            complement(654..1769)
                     /locus_tag="VFO88_01885"
     CDS             complement(654..1769)
                     /locus_tag="VFO88_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET9544306.1"
                     /translation="MTGVQLEIQAIAAVTAAACALPGAFLVLRRLSLLSDAIGHSILL
                     GIVLAFLVVQELTSPFLVVGAALVGLLTVVAVELVRGTGLVREDAAIGLVFPALFSLG
                     VVLVARYTDSVHLDTDAVLLGELAFTPFDRLIVGGWDLGPRALWVMSVILAVNLVALL
                     LFYKELKLTTFDPALALALGLAPGLVHYGLMTLVSVTAVGAFTAVGAILVVALMIVPP
                     ATAYLLTDRLGVLLGASVGIGVASAIGGYWLAHALDASIAGSMAATAGALFALAFFFA
                     PGRGVVAAARRRARQRWEFPQTMLAIHLLNHEGLPEAAVENRIDHLDAHLGWEPAFAH
                     EVVERAERRGLVLSDGAALELTDRGRAGARSALARTA"
     gene            complement(1766..2869)
                     /locus_tag="VFO88_01890"
     CDS             complement(1766..2869)
                     /locus_tag="VFO88_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET9544307.1"
                     /translation="MDFLGDYTLQIVALGAGTLGIVAGALGAFAVLRRQSLLGDAISH
                     AALPGVVLAFILTGTKAPLGLLLGAAFAGWAATLAVMAIARMSRIKYDAALGIVLSVF
                     FGIGLLLLTDVQKQPDATQAGLDRFLFGQAAGLLRSDVITMMALGLPVLVALALLWKE
                     FKLLAFDPGFGASLGLPMRALDVVLTGLLVVAIVVGLQTVGVVLMSAMVVAPAAAARQ
                     WTDRLGTMVVLSAGFGALAGVVGAVISSRVERMPAGPTIVLCVTVLVLVSLFLAPRRG
                     LLWARVRAHRARARLRVQAVLEALLDLDARHGDGHAHSEAVLEVAGAGRGGAGRTLAE
                     LERRGWAERRGDDAWLLTPAGRTEAEERYGGPR"
     gene            complement(2915..3691)
                     /locus_tag="VFO88_01895"
     CDS             complement(2915..3691)
                     /locus_tag="VFO88_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET9544308.1"
                     /translation="MSVRDGHAIEVTDLTVAYGDHPVLWDVDLDVPHGVLMGVVGPNG
                     AGKTTLVKAILGLVRPAAGRVSVLGRPYEDVRRAVAYVPQRGSVDWDFPTSVLDVVQM
                     GRYGALGWVRRPGRAERERSLEAIRKVGLEELAGRQISQLSGGQQQRVFLARALVQDA
                     DVYLMDEPFQGVDATTERAVVELLRELRAEGKTVVAVHHDLQTVPEYFDWVTLLNVRR
                     IASGPVDDAFTEENLRLAYGAPLGFLGRNGRPPEEGLVSE"
     gene            complement(3688..>4057)
                     /locus_tag="VFO88_01900"
     CDS             complement(3688..>4057)
                     /locus_tag="VFO88_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET9544309.1"
                     /translation="TVPPEKRVLITAHDAFLYFGRAYGFEVRGLQGISTVSEAGAADV
                     NDLAEFIAEEEIPAVFVESSVPQQTIRAVQEAVRARGFDVEIGGELFSDAMGDPGTPE
                     GTYPGMVRHNIETIVEALNG"
BASE COUNT          629 a         1494 c         1460 g          474 t
ORIGIN      
        1 gcgtccgtgc tccgctcctt cgaggagcgg gacgcgtacc tcgaggacat cccggtcgcg
       61 gacaccgtgc tcgtgctgga gctcctcgcc gccgagcgcg gcctcgccgc cggcctccgc
      121 tcgccgctcc tcccgcgcga gtagcgccgc ggcccgacgg gtgcggcagc cgccccgaga
      181 gaccgaaacg gagaaaaggg cggtgattct ccgcccctcc tccgtttctg catcgaccta
      241 cgcgcgccga acgaacgaga ggtagaccgt tccgatgccc gacgccgtgc actcgacgac
      301 gtcgaggctg cccaccgtcg gcgacgtctg gcggatcgcg atcagccgtt gcccgtcgac
      361 ctcggcgaac ccgatcgccc cgacgaccgt cgcttcgaac gaagccatac caggacatgt
      421 gaccgtgaac gcaagagcgt tgggattgcg cgtcgggtcg gccgaggccg ttggcgctgc
      481 cgcgatggca gtaaccagcg ccgcgccgca cacgaacgcg cgaagtttcg atcgttgccg
      541 cataaggatc ctcctcgtag ccgcagatac gaagtcggag aataggagga tccgcgaagc
      601 ggttcaatgg ccggagcctt ctgcgtgccg ccgatggttc cggctcgttc gcgctaggcc
      661 gttctcgcga gggccgagcg ggcgccggca cggccgcggt cggtcagctc gagcgccgcc
      721 ccgtcggaga ggacgagccc gcgccgctcc gcgcgctcga cgacctcgtg cgcgaacgcc
      781 ggctcccagc cgaggtgcgc gtccagatgg tcgatccggt tctcgaccgc cgcctccggg
      841 aggccctcgt ggttcaggag gtggattgcg agcatcgtct gcgggaactc ccaccgctgc
      901 cgagcccgcc gccgcgccgc cgcgacgacg ccgcgtccgg gcgcgaagaa gaacgcgagg
      961 gcgaagagcg cgcccgccgt agccgccatc gacccggcga tcgaggcgtc gagcgcgtgc
     1021 gcgagccagt agccgccgat cgcgctcgcg acgccgatcc ccacgctcgc gccgagcagc
     1081 acgccgagcc ggtcggtgag gaggtacgcg gtcgcgggcg gcacgatcat gagcgcgacg
     1141 acgaggatcg ccccgacggc cgtgaacgcg ccgaccgcgg tgaccgagac gagcgtcatg
     1201 agcccgtagt ggacgagccc cggcgcgagg ccgagcgcga gcgcgagcgc cgggtcgaac
     1261 gtcgtcagct tgagctcctt gtagaagagg aggagcgcga cgaggttcac ggcgaggatc
     1321 acgctcatca cccagagcgc gcgcggcccg aggtcccagc cgccgacgat cagccggtcg
     1381 aacggcgtga acgcgagctc gccgagcagc accgcgtccg tgtccaggtg caccgagtcg
     1441 gtgtaccggg cgacgagcac gacgccgagc gagaagagcg cggggaagac gaggccgatc
     1501 gccgcgtcct cgcgcacgag gcccgtcccg cggacgagct cgacggcgac gacggtcagc
     1561 agcccgacga gcgccgcccc gacgacgagg aacggcgagg tcagctcctg cacgacgagg
     1621 aacgcgagca cgatcccgag caggatcgag tggccgatcg cgtcggagag gagcgagagc
     1681 cggcgcagga cgaggaacgc gccgggaagc gcgcacgccg ccgccgtcac cgccgcgatc
     1741 gcctggatct cgagctggac gccggtcacc gcggccctcc gtagcgttcc tcggcctccg
     1801 tgcgccccgc gggcgtgagg agccaggcgt cgtccccgcg ccgctccgcc cagccgcggc
     1861 gctcgagctc ggcgagcgtc cggcccgcac cgccgcggcc cgcgcccgcg acctcgagca
     1921 ccgcctccga gtgggcgtgg ccgtcgccgt ggcgggcgtc gaggtcgagg agcgcctcga
     1981 gcaccgcctg cacgcgcagc cgcgcgcgcg cccggtgcgc ccgcacccgt gcccagagga
     2041 gcccgcggcg cggcgcgagg aagagcgaga cgagcacgag caccgtcacg cagaggacga
     2101 tcgtcgggcc cgccggcatc cgttcgacgc ggctcgagat cacggcgccc acgacgcccg
     2161 cgagggcgcc gaagccggcc gagagcacga ccatcgtgcc gagccggtcg gtccactgcc
     2221 gcgccgccgc cgcgggcgcg acgaccatcg cgctcatgag cacgaccccg accgtctgca
     2281 gtccgacgac gatcgccacg acgagcagcc cggtgaggac gacgtcgagc gcgcgcatcg
     2341 gcagcccgag gctcgccccg aagccggggt cgaacgcgag cagcttgaac tccttccaga
     2401 ggagcgcgag cgcgacgagc accggcagcc cgagcgccat catcgtgatc acgtcgctcc
     2461 gcagcaggcc tgccgcctgg ccgaacagga agcggtcgag ccccgcctgc gtcgcgtccg
     2521 gctgcttctg cacgtccgtc aggaggagca ggccgatccc gaagaagacc gagaggacga
     2581 tcccgagcgc ggcgtcgtac ttgatccgcg acatgcgggc gatcgccatg acggcgagcg
     2641 tggccgccca gccggcgaag gcggcgccga ggaggagccc gagcggcgcc ttcgtccccg
     2701 tgaggatgaa cgcgagcacg acgcccggga gcgccgcgtg cgagatcgcg tctccgagca
     2761 ggctctggcg gcgcagcacg gcgaaggcgc cgagcgcgcc ggcgacgatc ccgagcgtcc
     2821 cggctccgag cgcgacgatc tgcagcgtgt agtcgccgag gaagtccacg cgcttactcg
     2881 gcctcggctc gaaacgaagc cttgcgacga agcgtcattc cgagacgagg ccttcctccg
     2941 gcggtcggcc gttccggccg aggaacccga gcggggcgcc gtacgcaagg cggaggttct
     3001 cctccgtgaa cgcgtcgtcg accgggccgc tcgcgatccg gcggacgttc aggagcgtga
     3061 cccagtcgaa gtactccggc acggtctgga ggtcgtggtg gacggcgacg acggtcttcc
     3121 cctcggcgcg cagctcgcgc aggagctcga cgacggcgcg ctccgttgtc gcgtccacgc
     3181 cctggaaggg ctcgtccatg aggtacacgt ccgcgtcctg cacgagcgcc cgcgcgagga
     3241 agacacgctg ctgctggccg ccggagagct ggctgatctg gcgcccggcg agctcctcga
     3301 gcccgacctt gcggatcgcc tcgagcgagc gctcgcgctc ggcccggccg gggcgccgca
     3361 cccagccgag cgcgccgtag cggcccatct ggacgacgtc gaggacgctc gtcgggaagt
     3421 cccagtcgac cgagccgcgc tgcgggacgt acgcgacggc gcggcgcacg tcctcgtacg
     3481 ggcggcccag cacggacacg cggcccgcgg ccgggcgcac gaggccgagg atcgccttga
     3541 ccagcgtggt cttccccgcg ccgttcggcc cgacgacgcc catgaggacg ccgtgcggca
     3601 cgtcgaggtc gacgtcccag aggacggggt ggtcgccgta cgcgacggtg aggtcggtga
     3661 cctcgatcgc gtgcccgtcg cgcacgctca tccgttcagc gcctccacga tcgtctcgat
     3721 gttatggcga accatcccgg gatacgtgcc ctccggcgtg cccgggtcgc ccatcgcgtc
     3781 ggagaagagc tcgccgccga tctccacgtc gaacccgcgc gcgcgcaccg cctcctgcac
     3841 ggcgcggatc gtctgctgtg ggacggacga ctcgacgaag accgcaggga tctcctcctc
     3901 cgcgatgaac tcggccaggt cgttcacgtc cgcggcgccc gcctccgaga cggtcgagat
     3961 gccctggagc ccgcgcacct cgaacccgta cgcccgcccg aagtagagga acgcgtcgtg
     4021 ggcggtgatg aggacgcgct tctccggcgg gacggtc
//
LOCUS       DASTFC010000065         5092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1138340,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000065 DASTFC010000000
VERSION     DASTFC010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138340"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFO88_01905"
     CDS             complement(<1..486)
                     /locus_tag="VFO88_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET9544310.1"
                     /translation="MKTIRLLLVALCAVVFLSACSSGDDGEASGDAAVEGEVGAVAEG
                     DGGGEGGASDEAAVPVAELPGAGPKVIQTATLGVVVEEGRFDDAVREARGIAARLGGF
                     VVSSQTQRGDEDVPRSGMLVLRVPGESYAAAMDALGRLGEIDHESEEGTDVSQEYVDL
                     EA"
     gene            528..953
                     /locus_tag="VFO88_01910"
     CDS             528..953
                     /locus_tag="VFO88_01910"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9544311.1"
                     /translation="MDGFGFSADIRVRFSETDAQGIANNAVYLNWFEVARVAYLARFR
                     GGYRGMIEEGFEAVTLETHVRYRQPTRFDDELRIWTRIAEPERLRARFRFEYALERLS
                     EPAGVVADGWTSHATVDATTLSPVRMPDWLREAIRGVER"
     gene            complement(950..2869)
                     /locus_tag="VFO88_01915"
     CDS             complement(950..2869)
                     /locus_tag="VFO88_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9544312.1"
                     /translation="MRAEVDAQPVRERRPARRKAFLRSVPWKRLGLGLLIALVVATLA
                     LGLGFAGSAARIPAGVTVAGMDVAGKTPAEAAALLEARAAELADVPVVFTVGDERFPL
                     SPADLEARVDWNAVAAEARAEGDWPHPFRGLKRLSLRVFGADVEPAAEVYEARLAYEL
                     DRMAKEVDRPARDAAIVLRGMEPALVPDRVGTTLARRESADMIVHAVAGFDREPVPLG
                     VTTAHPSVEAAELEPVLAEVRTVLSAPVRFSWKGAAWGVQPGQLADFLLLPADGRSAL
                     EIGGKAADRYFDRLARSVNRRPREAGFTVAADGRTVRVVESRTGQQVDAEASGEALLA
                     GARSPNRREAALVVATVEPELTTEEARSLGITRVLSSYTTAYAGTADRITNLTLAVKA
                     IHGTQLAPGTPFSFNEVVGPRTERRGYRPAPTIIDNEYEDTVGGGVSQVATTVFNAAW
                     EAGVKITDRTPHSLYIDRYVAGRDATVTYPEIDLGFENDTDGWIVMQGIPGDTGITIR
                     LLGGETDRRVESDPGFLRITEKAEVETVPDPTLFVGERVVEEVGAPARTITVRRTVYE
                     GGEVLYEEAWVTSYDSIPRVVRVGTIPKPKPPPPPPPPPPGPPPPPPPGPPPPGPPPP
                     PPPPPPPTTTGSGRR"
     gene            complement(2904..3830)
                     /locus_tag="VFO88_01920"
     CDS             complement(2904..3830)
                     /locus_tag="VFO88_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9544313.1"
                     /translation="MPLPVAAFAQALLAAAGLGGASPPVEDPLAGLTARDKAALVVVS
                     GLPAPRGVGGVFVRSWNADAPRPRDTLVFVDQEGGEVRSFPALPPAPWPSEHATAGEA
                     RAAGRETGAALRRAGVHVDFAPVLDLAGGPLGARHFRRPELGVAFARGLGEGGVVACV
                     KHFPGLGSAEHSTDDRPWVLSRFRRAELAAFRAAIRAGVPCVMTSHAVYRELGFARAL
                     NSPEAHRMLRDWGFEGVVVTDSLSIFSSGPWPVEWARRALRAGGDLVLYTSPEHARRA
                     IRALVPLARAGELDEAVLRVLELRRRAGLPPP"
     gene            3848..5059
                     /locus_tag="VFO88_01925"
     CDS             3848..5059
                     /locus_tag="VFO88_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9544314.1"
                     /translation="MDFDLTPEQREVQATVRQFVDERILPTAIENDINHHLDMEAIEG
                     MAELGLLGIVIPEEYGGAGMDFVCEALSCEEIERGEAAFRTLISVHVGLNSLSLLRYG
                     TEEQKERYLTPQAKGEKIACFGLTEPAAGSDVAAMKTTARREGDTYVLNGQKNWISYA
                     SVADHALVFAKTDPEAGHKGISAFAIETAWPGVSAADTENKLGIWAGSTGELFFENVE
                     VPAESLVGEEGQGFEIAMYGLDQGRFTVAAGAVGVIRACLERSVEYARERETFGQPIG
                     KYQFVQDMIAEMVLGYETSKLLVLQAAWMKDEGLRNTRETSLAKWHATESAFKAAHLA
                     IQVHGSYGYSAEYGIERYFRNARAPIIYEGTTQIHKMMQAEHALGLRRLNGHTGEMSP
                     IVSWGPALAAR"
BASE COUNT          703 a         1871 c         1904 g          614 t
ORIGIN      
        1 cgcctcgagg tcgacgtact cctgcgagac gtcggtcccc tcctcgctct cgtggtcgat
       61 ctcgccgagc cggccgagcg cgtccatcgc ggcggcgtac gactcgccgg gcacgcggag
      121 aacgagcatg ccgctccgcg gcacgtcctc gtcgccgcgc tgggtctgcg agctgacgac
      181 gaacccgccg agccgggcgg cgatcccgcg cgcctcgcgg acggcgtcgt cgaagcggcc
      241 ctcctcgacg acgacgccga gggtcgcggt ctggatcacc ttcgggccgg cgccgggaag
      301 ctcggcgacc ggaacggccg cctcgtcgga ggcgccgccc tcgccgccgc cgtcgccctc
      361 cgccacggcg cccacctcgc cctcgacggc ggcgtcgccc gacgcctccc cgtcgtcgcc
      421 ggaggagcag gccgagagga acacgactgc gcagagcgcc acgaggagga gccggatcgt
      481 cttcatgctt gctccgacgg gccgtgcgct gcgaaagttc ccggcccgtg gacgggttcg
      541 gcttctccgc agacatccgc gtccgcttca gcgagacgga cgcgcagggc atcgcgaaca
      601 acgcggtgta cctgaactgg ttcgaggttg cgcgcgtcgc gtacctcgcg cgctttcgcg
      661 gcggctaccg cgggatgatc gaggagggct tcgaggcggt cacgctcgag acgcacgtcc
      721 gctaccgcca gccgacgcgc ttcgacgacg agctccggat ctggacgcgg atcgccgagc
      781 ccgagcgcct gcgcgcccgc ttccgcttcg agtacgcgct cgagcggctg agcgagcctg
      841 cgggcgtcgt tgccgacggc tggacgagcc acgcgaccgt cgacgcgacg acgctctcgc
      901 ccgtgcggat gccggactgg ctccgcgagg cgatccgcgg cgtcgagcgc tagcggcgac
      961 cgctgccggt cgtcgtcggc ggcggcggag gcggcggcgg aggcgggggg ccgggcggcg
     1021 gcgggccagg cggcgggggc ggcggcggcc cgggcggcgg cggcggcgga ggcggcggag
     1081 gcttcggctt cgggatcgtc ccgacgcgga cgacgcgcgg gatcgagtcg tagctcgtca
     1141 cccacgcctc ctcgtagagc acctcgccgc cctcgtagac cgtccggcgc acggtgatcg
     1201 tccgggcggg ggcgccgacc tcctcgacga cccgctcgcc gacgaagagc gtcgggtccg
     1261 gaaccgtctc gacctccgcc ttctcggtga tccggaggaa gccggggtcg gactcgacgc
     1321 gcctgtccgt ctcgccgccg aggagccgga tcgtgatgcc ggtgtcgccg gggatgccct
     1381 gcatgacgat ccagccgtcc gtgtcgttct cgaacccgag gtcgatctcg gggtaggtga
     1441 ccgtggcgtc gcgcccggcg acgtagcggt cgatgtagag cgagtgcggg gtccggtcgg
     1501 tgatcttcac cccggcctcc cacgccgcgt tgaagacggt cgtcgccacc tgggagacgc
     1561 cgccgccgac ggtgtcctcg tactcgttgt cgatgatcgt cggcgccggc cggtagccgc
     1621 ggcgctccgt ccgcgggccg acgacctcgt tgaaggagaa cggcgtgccg ggcgcgagct
     1681 gtgtcccgtg gatcgccttc acggcgagcg tgaggttcgt gatccggtcg gcggtccccg
     1741 cgtacgccgt cgtgtacgag gagaggaccc gcgtgatccc gagcgagcgc gcctcctcgg
     1801 tcgtcagctc cggctcgacc gtcgcgacga cgagcgccgc ctcgcgccgg ttcggcgagc
     1861 gcgcgccggc gagaagcgcc tcgccgctcg cctcggcgtc gacctgctgg cccgtgcggc
     1921 tctcgacgac gcggaccgtc cggccgtcgg ccgcgacggt gaagcctgcc tcgcgcggcc
     1981 ggcggttcac cgagcgggcg aggcggtcga agtagcggtc ggcggccttc ccgccgatct
     2041 cgagcgccga ccgcccgtcg gccgggagca ggaggaagtc cgcgagctgg ccgggctgga
     2101 caccccacgc cgcgcccttc cagctgaacc gcacgggcgc agagagcacc gtccgcacct
     2161 cggcaagaac cggctcgagc tcggccgcct cgacggacgg gtgcgcggtc gtgacgccca
     2221 gcgggaccgg ctcccggtcg aaccccgcga ccgcgtggac gatcatgtcg gccgactcgc
     2281 gccgagccag ggtggtgccg acgcggtcgg ggacgagggc cggctccatc ccgcgcagca
     2341 cgatcgcggc gtcgcgggcg gggcggtcga cctccttcgc catcctgtcg agctcgtacg
     2401 cgagccgcgc ctcgtagacc tcggcggcgg gctcgacgtc cgcgccgaag acgcgcagcg
     2461 agagccgctt gagcccgcgg aacgggtgcg gccagtcgcc ctcggcgcgc gcctccgcgg
     2521 cgacggcgtt ccagtccaca cgcgcctcga ggtcggccgg cgagagcggg aagcgctcgt
     2581 cgccgaccgt gaagacgacc gggacgtcgg cgagctcggc cgcgcgcgcc tcgagcaggg
     2641 ccgcggcctc ggcgggcgtc ttgccggcga cgtccatccc ggcgacggtc acgccggcgg
     2701 ggatccgcgc ggccgagccg gcgaagccga gcccgagcgc gagcgtggcg acgacgagcg
     2761 cgatgaggag gccgagcccg aggcgcttcc acgggaccga gcgaaggaac gccttccggc
     2821 gcgcaggacg gcgctcgcgc accggctggg cgtcgacctc ggcccgcaag gcggccaatc
     2881 tagcagcgcg cgtcgggact tcgtcagggc ggcggaaggc ccgcccggcg ccgcagctcg
     2941 agcacgcgga ggacggcctc gtcgagctcg ccggcgcgcg cgagtggcac gagcgcgcgg
     3001 atcgcgcgtc gcgcgtgctc ggggctcgtg tagaggacga ggtcgccgcc tgcgcgaagc
     3061 gcccggcgcg cccactcgac gggccacggg ccgctcgaga agatgctcag cgagtcggtc
     3121 acgacgacgc cctcgaaccc ccagtcgcgc agcatccgat gggcctcggg cgagttcagg
     3181 gcccgcgcga agccgagctc ccggtagacg gcgtggctcg tcatgacgca cgggacgccg
     3241 gctcggatcg ccgcgcgaaa ggcggccagc tccgcacggc ggaaccgcga cagcacccac
     3301 ggccggtcgt ccgtcgagtg ctcggccgac ccgaggcccg ggaagtgctt cacgcacgcg
     3361 acgacgccgc cctcgccgag cccgcgcgcg aacgcgacgc cgagctccgg ccggcggaag
     3421 tggcgcgcgc cgagcggccc gcccgcgagg tcgaggacgg gcgcgaagtc cacatggacg
     3481 ccggcgcgcc ggagcgccgc gcccgtctcc cttccggccg cgcgcgcctc cccggccgtc
     3541 gcgtgctcgg acggccacgg ggccggcggc agcgcgggga acgaacgcac ctcgcccccc
     3601 tcctggtcga cgaagacgag cgtgtcgcgc ggccggggcg cgtcggcgtt ccacgagcgc
     3661 acgaagacgc cgccgacgcc gcgcggcgcc gggaggcccg agacgacgac gagcgccgcc
     3721 ttgtcccgcg ccgtgagccc cgcgagcgga tcctccaccg gaggggaggc gccgccgagc
     3781 ccggccgccg cgaggagggc ttgcgcgaac gcggcgacgg gcagcggcac gtcactagac
     3841 tagccccgtg gatttcgacc tcacccccga gcaacgcgag gtgcaggcga ccgtccgaca
     3901 gttcgtggac gagcgcatcc tgccgaccgc gatcgagaac gacatcaacc accacctcga
     3961 catggaggcg atcgagggca tggccgagct gggcctgctc gggatcgtca tccccgagga
     4021 gtacggcggc gccggcatgg acttcgtctg cgaggcgctc tcgtgcgagg agatcgagcg
     4081 cggcgaggcg gcgttccgaa cgctcatctc ggttcacgtc gggctgaact cgctctcgct
     4141 cctgcgctac ggcacggagg agcagaagga gcgctacctg acgccccagg cgaagggcga
     4201 gaagatcgcc tgcttcggcc tgaccgagcc cgcggcgggc tccgacgtcg cggcgatgaa
     4261 gacgaccgcg cggcgcgagg gcgacacgta cgtcctgaac ggccagaaga actggatctc
     4321 gtacgcgtcc gtcgccgacc atgcgctcgt cttcgcgaag accgacccgg aggccggcca
     4381 caagggcatc tccgcgttcg cgatcgagac cgcctggccg ggcgtctccg cggccgacac
     4441 cgagaacaag ctcggcatct gggcgggctc gacgggcgag ctcttcttcg agaacgtcga
     4501 ggtgccggcc gagagcctgg tcggcgagga gggacagggc ttcgagatcg cgatgtacgg
     4561 gctcgaccag ggccgcttca ccgtcgccgc gggcgccgtc ggcgtcatcc gcgcctgcct
     4621 ggagcgctcc gtcgagtacg cgcgcgagcg ggagacgttc ggccagccga tcggcaagta
     4681 ccagttcgtc caggacatga tcgcggagat ggtgctcggc tacgagacct cgaagctgct
     4741 cgtcctgcag gccgcgtgga tgaaggacga gggcctccgc aacacgcgcg agacctcgct
     4801 cgccaagtgg cacgcgaccg agtccgcgtt caaggcggcg cacctcgcga tccaggtgca
     4861 cggctcgtac ggctactccg ccgagtacgg catcgagcgc tacttccgga acgcgcgcgc
     4921 gccgatcatc tacgagggca cgacgcagat ccacaagatg atgcaggccg agcacgcgct
     4981 cgggctgcgc cgcctcaacg gccacaccgg cgagatgtcg cccatcgtgt cctggggccc
     5041 cgcgctcgcg gcgcggtagc gtcgggagcc gggcctccgg ctacgccgac cc
//
LOCUS       DASTFC010000066         6831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_133073,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000066 DASTFC010000000
VERSION     DASTFC010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133073"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFO88_01930"
     CDS             complement(<1..348)
                     /locus_tag="VFO88_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544315.1"
                     /translation="MAIGVESGLVRSGGLLPEDPYVETYIVRAGGATVFDVGPDERVT
                     LTDPEGGQAAELTVLGPDGRDAAAALGVRADAEASVVRAALRSPGAYGFLTELAARGL
                     DPHDAVALRLFGET"
     gene            complement(348..1754)
                     /locus_tag="VFO88_01935"
     CDS             complement(348..1754)
                     /locus_tag="VFO88_01935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9544316.1"
                     /translation="MTEQMWGGEPWWGVGEEWNPDWFLTDRQKELRATLIELCEQEMR
                     ANAKRSDDELLYPRRNLELLGEHGFLGLTVPEEYGGLGENHVAYSMVCETIARYGCAS
                     TAMCYVMHIGGVNAIMLRPTDELVERYIRPLRDGKIGTLSYSDPETGSHFWYPISSGA
                     ERVNGGFKVRKKASWTTSAGFADFYVVQTTSPDFSGYDDLSVFVIDGEDVEAQPALWD
                     ALGLRGNQSGPLEVPDVEIPESQIVGPVGDGAASNDESVDPWFLIGSSSVWNGIALGA
                     LDIAKRHTTRKRHVDVGLRVADYPTIQDYVGEAVIDTNACRVFVLSVANAMDKATDDN
                     TKLLKPGEFARADFLHWAWQIKFAAAKNVAHVVDKMLHACGGSGYKRDMELERYLRDA
                     KAGWVMGPTNEVLRQFVGKAVLLGFDVLDYWNQTFNRRAVENEVKKLDAKGKRELAEQ
                     LISQADEQEAKQAQPAAS"
     gene            complement(1812..3122)
                     /locus_tag="VFO88_01940"
     CDS             complement(1812..3122)
                     /locus_tag="VFO88_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9544317.1"
                     /translation="MTDFLRLAAIPLLVLGNAVFVAAEYALVTARRSRLEEQARRGRR
                     GARTALDLMDQPVRFISTVQVAITVFGILLGALGEPIVSSLFDEALPRGVSFLIAFLV
                     LTYVGVVLGELVPKAVALENADRLAAVLAVPVSWLSRALHPLVWVLDSSAGLVARLLR
                     VPPARAGITSHTEEDIRFIVGEAEDMGAIETVEEEMVYKVFDFADKEAHEVMVPRPQV
                     VALSADLPTQEALGALVGSPYTRYPVYRDSLDDVLGILHVRDLFRALYDRGIENVEME
                     ELIRPAYVVPETKDLGALLAEFRTQNQHMAIVVDEYGGVAGIVTLEDLLEEIVGEIED
                     EYDLPDESVERLDERRIRIAGTFPIDDFNEQFGQEIPQEDFHTVAGFVFGELGRAPGE
                     GDEILWNGLRFDVVETDGTRIGKLEVEFIPPEEQAEAEAEPAAG"
     gene            3326..3922
                     /locus_tag="VFO88_01945"
     CDS             3326..3922
                     /locus_tag="VFO88_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544318.1"
                     /translation="MADDHEVVREGLRLSLSRAPHIRVVGEASDGASAVSLAERRRPN
                     VVIMDVRMPGMDGLEATKLLTDKVPSTSVLIFTAYGERSLLGRGLESGAKGYILKEAP
                     HQTLLRAIEKVASGEGYVDPALMPDFLTRDREDMLTGREREILKLLADGMSNADVAAR
                     LFISQETVKSHVRHILAKLEADTRTHAVAIALREAIID"
     gene            3932..5134
                     /locus_tag="VFO88_01950"
     CDS             3932..5134
                     /locus_tag="VFO88_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9544319.1"
                     /translation="MTALVRRIGPAGLLVAGAAVWATVGGLGLVVGSAQLAAGALVAA
                     TAFAGAFAVFLIVSERRRHEGVEDELQSQSRFLESLVGSIASVSDTREPAEILERTLT
                     ESRRLFDAETAELVPPEQAVVRDAHEVEGGIRVPLRAGSTPLGMLELRRSEPFHRVDL
                     MRATMLADFASRAYENARLIEEAREREDERTRLTERLITAEQDERRRLSIFLHDGPLQ
                     SMSGIALMHDAALAALQDGRYEEASKVIENSLERERDTIRTLRDLSFAIEPLVLRDRG
                     FAAAVDALAEQIEGSHRITVSADVTAGEQLAAKAQVALYQLIREAVSQAVRRRPSRIG
                     VTVAANGEGFTTAIGDDGMGERRRGGIEELDERVRVLNGRVSVETGDDGGTTVRVVLP
                     PYAGAGPG"
     gene            5146..5337
                     /locus_tag="VFO88_01955"
     CDS             5146..5337
                     /locus_tag="VFO88_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544320.1"
                     /translation="MSDGKGHLLFVSKSSGYELAEREGDPPAPGSSVEDGEKTFRVSK
                     LAPSPLPGDDRVCAYLEPA"
     gene            complement(5334..5651)
                     /locus_tag="VFO88_01960"
     CDS             complement(5334..5651)
                     /locus_tag="VFO88_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HET9544321.1"
                     /translation="MPRAALVVCVVLLAAACSSDAPTGAPNYEGVVVSVGRTNVEIRH
                     DADACGQIVFEAMDGVAVRRGDDEIGWADLRAGDRIQVWAPGVDESCPAYGGAERIEI
                     TGP"
     gene            complement(5659..6126)
                     /locus_tag="VFO88_01965"
     CDS             complement(5659..6126)
                     /locus_tag="VFO88_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544322.1"
                     /translation="MRSGRRTLARAAPVVTAVALLPGAALPLGCGGDCTSYGCVDGVA
                     FAFRDGLLPSSAAAPLRAHVCFGGRCDSFTGTPRELREVLAYDFLNGGSIDGAPLDPE
                     REYEAALRVTDATGRTIVRAERQVTPTREEGDWCVPECTEARVVFTRADVVGG"
     gene            complement(6157..>6831)
                     /locus_tag="VFO88_01970"
     CDS             complement(6157..>6831)
                     /locus_tag="VFO88_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9544323.1"
                     /translation="ALPDGAPLVLATGGFAAGRGLVREHVTPEAEHLALRSNPWSTGD
                     GLRLGLAAGASLSDGMDEFWGRAMAAPPAVVEPATFRPLAQFYARHARIVDERGKEFV
                     ARTWSEIDVAQWIARRPRARAWFHVPDEKLGERVRDRTVGEMVEAARAAGTFVERRDG
                     ETVVEVVAGITTTLGGLAIDTEARVADGVWAVGADAGGISTGGWASGLATALVLGLVA
                     AESAAA"
BASE COUNT          975 a         2483 c         2421 g          952 t
ORIGIN      
        1 ggtctcgccg aagagccgga gcgcgacggc gtcgtgcggg tcgagcccgc gcgccgcgag
       61 ctccgtgagg aagccgtacg cgcccggcga gcggagcgcc gcgcgcacga cggaggcctc
      121 cgcgtccgcg cgaacgccca aggcggccgc cgcgtcgcgc ccgtccgggc cgagcaccgt
      181 cagctcggcc gcctgcccgc cctcgggatc cgtgagcgtg acgcgctcgt ccggcccgac
      241 gtcgaagacc gtcgcgccgc cggcccgcac gatgtacgtc tcgacgtacg ggtcctcggg
      301 caggagcccg ccggagcgga cgagccccga ctcgaccccg atcgccatca gctcgcggcc
      361 ggctgcgcct gcttggcttc ctgctcgtcc gcctgggaga tgagctgctc cgcaagctcg
      421 cgcttgccct tcgcgtcgag cttcttcacc tcgttctcga ccgcgcggcg gttgaacgtc
      481 tggttccagt agtcgagcac gtcgaagccg agcaggacgg ccttgccgac gaactggcgc
      541 agcacctcgt tcgtcgggcc catgacccag ccggccttcg cgtcgcggag gtagcgctcg
      601 agctccatgt cgcgcttgta gccggagccg ccgcacgcgt ggagcatctt gtccacgacg
      661 tgcgcgacgt tcttcgcggc ggcgaacttg atctgccagg cccagtggag gaagtccgcg
      721 cgcgcgaact cgcccggctt caggagcttc gtgttgtcgt ccgtcgcctt gtccatcgcg
      781 ttcgcgaccg agaggacgaa cacccggcac gcgttcgtgt cgatcacggc ctcgccgacg
      841 tagtcctgga tcgtcgggta gtcggcgacg cgcaggccga cgtccacgtg gcgcttgcgc
      901 gtcgtgtggc gcttcgcgat gtcgagcgcg ccgagcgcga tcccgttcca gacggacgac
      961 gatccgatga ggaaccacgg gtcgacggac tcgtcgttcg aggccgcgcc gtcgccgacg
     1021 gggccgacga tctgcgactc cgggatctcg acgtcgggca cctcgagcgg ccccgactgg
     1081 ttgccgcgca gcccgagcgc gtcccagagc gccggctgcg cctccacgtc ctccccgtcg
     1141 atcacgaaga cggagaggtc gtcgtagccg gagaagtcgg gcgaggtcgt ctggacgacg
     1201 tagaagtcgg cgaagccggc cgacgtcgtc cacgacgcct tcttgcgcac cttgaagccg
     1261 ccgttcacgc gctcggcgcc ggacgagatc ggataccaga agtgcgagcc cgtctcgggg
     1321 tcggagtacg agagcgtccc gatcttcccg tcgcggagcg gccggatgta gcgctcgacg
     1381 agctcgtcgg tcgggcggag catgatcgcg ttcacgccgc cgatgtgcat gacgtagcac
     1441 atggccgtcg aggcgcaccc gtagcgcgcg atcgtctcgc agaccatcga gtaggcgacg
     1501 tggttctcgc cgagcccgcc gtactcctcg ggcaccgtga ggccgaggaa gccgtgctcg
     1561 ccgagcagct cgaggttgcg gcgcggatag agcagctcgt cgtccgagcg cttcgcgttc
     1621 gcgcgcatct cctgctcgca caattcgatc agggtcgcgc ggagctcctt ctgccggtcg
     1681 gtgaggaacc agtcggggtt ccattcctcg ccgacgcccc accacggctc gccgccccac
     1741 atctgctcgg tcatgtccac ctccgcctcg gtcgccttga gggcaatcgt atctgcggcg
     1801 agagtgcgct actacccggc tgcgggctcc gcctcggcct ccgcctgctc ctcgggcggg
     1861 atgaactcca cctcgagctt gccgatccgg gtgccgtcgg tctcgacgac gtcgaagcgc
     1921 aggccgttcc agaggatctc gtcgccctcg ccgggggcgc ggccgagctc gccgaagacg
     1981 aagccggcga ccgtgtggaa gtcctcctgc gggatctcct ggccgaactg ctcgttgaag
     2041 tcgtcgatcg ggaacgtccc ggcgatgcgg atgcgccgct cgtcgaggcg ctcgacggac
     2101 tcgtccggca ggtcgtactc gtcctcgatc tcgccgacga tctcctcgag gaggtcctcg
     2161 agcgtcacga tcccggcgac gccgccgtac tcgtccacca cgatcgccat gtgctggttc
     2221 tgggtgcgga actcggcgag gagcgcgccg agatccttcg tctccgggac gacgtacgcg
     2281 ggacggatga gctcctccat ctcgacgttc tcgatcccgc ggtcgtagag cgcgcggaag
     2341 aggtcgcgga cgtggaggat cccgagcacg tcgtcgagcg agtcgcggta gacgggatag
     2401 cgcgtgtacg gcgagccgac gagcgcgccg agcgcctcct gcgtcggcag gtcggccgag
     2461 agcgcgacga cctgcgggcg cgggaccatc acctcgtggg cctccttgtc cgcgaagtcg
     2521 aagaccttgt agaccatctc ctcctcgacg gtctcgatcg cgcccatgtc ctcggcctcg
     2581 ccgacgatga agcggatgtc ctcctccgtg tgcgaggtga tcccggcgcg cgccggcggg
     2641 acgcggagaa gccgggcgac gagcccggcc gaggagtcga gcacccagac gagcgggtgg
     2701 agggcacgcg agagccagct caccggcacc gcgagcacgg ccgccagccg gtcggcgttc
     2761 tcgagcgcga ccgccttcgg gacgagctcg ccgagcacga cgcccacgta cgtgagcacg
     2821 aggaaggcga tgaggaagga gacgccgcgc ggcagcgcct cgtcgaagag gctcgagacg
     2881 atcggctccc cgagggcgcc cagcaggatc ccgaacaccg tgatcgcgac ctggaccgtg
     2941 ctgatgaagc gcaccggttg gtccatcagg tcgagtgccg tgcgcgcgcc gcggcggccg
     3001 cgcctggctt gctcctcgag ccggctccgg cgcgcggtca cgagcgcgta ctcggccgcg
     3061 acgaacacgg cgttccccaa gacgagcagg gggatcgccg cgaggcgaag gaaatcggtc
     3121 acgccccgct ctccggggcc tgggtactgg gcggtttctc gtccaactcg gtgctctgat
     3181 ggtagcgcag catccggcgg attccgcatt cgcgggccgt tccctgcgcc gccggcgcag
     3241 gagccctaaa gtttcgggcg aaactgcccg aaactgaaga cgacctgggg agccgatcaa
     3301 actgccgcaa gaaatcacct gcctcatagc ggacgaccac gaggtcgtga gagaagggct
     3361 ccggctctcc ctctcccgcg cgccgcacat ccgcgtcgtc ggcgaggcct cggacggggc
     3421 gagcgccgtc tcgctcgccg agcgccgccg cccgaacgtc gtgatcatgg acgtccggat
     3481 gcccggcatg gacgggctcg aggcgacgaa gctcctcacg gacaaggttc cgtcgacctc
     3541 cgtcctgatc ttcaccgcgt acggcgagcg ctcgctcctc gggcgcgggc tcgagtcggg
     3601 cgcgaagggc tacatcctga aggaggcgcc gcaccagacg ctcctgcgcg cgatcgagaa
     3661 ggtcgcgagt ggcgagggct acgtcgaccc ggcgctcatg cccgacttcc tcacgcgcga
     3721 ccgcgaggac atgctgaccg gccgcgagcg cgagatcctg aagctccttg ccgacgggat
     3781 gtcgaacgcc gacgtggccg cccggctctt catcagccag gagacggtca agagccacgt
     3841 ccgccacatc ctcgcgaagc tcgaggcgga taccaggacc catgccgtcg cgatcgcgct
     3901 ccgcgaagcg atcatcgatt aggggcgttc ggtgacggcg ctcgtccggc gcatcgggcc
     3961 ggcgggcctg ctcgtggccg gcgcagccgt gtgggcgacg gtcggcgggc tcgggctcgt
     4021 cgtcgggagc gcccagctcg ccgccggcgc gctcgtcgcc gcgaccgcgt tcgccggcgc
     4081 gttcgcggtg ttcctcatcg tctccgagcg ccgccgccac gagggcgtcg aggacgagct
     4141 ccagtcgcag tcccgcttcc tcgagtcgct cgtcggctcg atcgcctccg tctcggacac
     4201 gcgcgagccg gcggagatcc tcgagcgtac gctcaccgag tcgcgccgcc tcttcgacgc
     4261 cgagacggcg gagctcgtcc ctccggagca ggccgtcgtc cgcgacgcgc acgaggtcga
     4321 gggcgggatc cgcgtgccgc tgcgcgccgg ctcgacgccg ctcgggatgc tcgagctgcg
     4381 ccgctcggag ccgttccacc gcgtcgacct catgcgggcg acgatgctcg ccgacttcgc
     4441 ctcgcgtgcg tacgaaaacg cgcggctgat cgaggaggcg cgcgagcgcg aggacgagcg
     4501 cacacgcctg accgagcgcc tcatcacggc cgagcaggac gagcggcggc ggctctcgat
     4561 cttcctccac gacgggccgc tccagtcgat gtccggaatc gcgctcatgc acgacgccgc
     4621 gctcgccgcg ctccaggacg ggcgctacga ggaggcgtcg aaggtgatcg agaactcgct
     4681 cgagcgcgag cgggacacga tccgcacgct ccgcgacctc tcgttcgcga tcgagccgct
     4741 ggtcctgcgc gaccgcgggt tcgcggcggc ggtcgacgcg ctcgccgagc agatcgaggg
     4801 ctcgcaccgc atcaccgtct cggccgacgt caccgccggg gagcagctcg ccgcgaaggc
     4861 ccaggtggcg ctctaccagc tcatccggga ggcggtgtcg caggccgtcc gtcgccggcc
     4921 gtcgcggatc ggcgtgaccg tggccgcgaa cggcgagggc ttcacgaccg cgatcggcga
     4981 cgacggcatg ggcgagcgcc gccgcggcgg aatcgaggag ctcgacgagc gtgtgcgcgt
     5041 tctgaacggc cgcgtgtcgg tcgagacggg cgatgacggc ggcacgacgg tccgcgtcgt
     5101 cctgcccccg tacgcgggcg ccgggccggg gtaagatccc ggccgatgag cgacggcaag
     5161 gggcacctcc tcttcgtctc gaagagctcg ggctacgagc tcgccgagcg cgagggcgac
     5221 ccgcccgcgc cgggctcgtc ggtcgaggac ggcgagaaga cgttccgcgt ctcgaagctc
     5281 gcgccgtccc cgttgcccgg cgacgaccgg gtctgcgcct acctcgagcc cgcttagggc
     5341 cccgttatct cgatccgctc ggcaccgccg tacgccgggc aagactcgtc gacgccgggt
     5401 gcccagactt gaatgcgatc gcccgcgcga aggtcggccc agccgatctc gtcgtcgccg
     5461 cgacgcacgg cgacgccgtc catcgcctcg aacacgatct gcccgcacgc gtccgcgtcg
     5521 tgacggatct cgacgttcgt ccggccgacc gagacaacga cgccctcgta gttcggggct
     5581 ccggtgggcg cgtcgctgct gcaggcggcg gcgagaagaa cgacgcagac gacgagggcc
     5641 gctcgcggca cgaatcgcct agccgcccac cacgtcggcc cgcgtgaaga ccacacgtgc
     5701 ttcggtgcat tcggggacgc accagtcgcc ctcctcgcga gtcggcgtga cctgccgctc
     5761 ggcgcggacg atcgtcctcc ccgtcgcgtc ggtgacgcga agggccgcct cgtactcacg
     5821 ttccggatcg agcggcgcgc cgtcgatcga cccgccgttc aggaagtcgt aggcgagcac
     5881 ctcccggagc tcgcggggcg tgcccgtgaa agagtcgcac cggccgccga agcagacgtg
     5941 cgcgcgaagc ggcgccgcgg cggacgaggg tagaagcccg tcgcggaacg cgaacgcgac
     6001 gccgtccacg catccgtacg aggtgcagtc tccgccgcag ccgagcggga gagcggcgcc
     6061 ggggaggagc gcgacggccg tcacgaccgg cgcggctcgt gcgagcgtcc ggcgcccgga
     6121 tctcatggag attcagacgc ctgagagcgc ctgaagtcag gccgccgcgc tctcggcggc
     6181 gacgaggccg aggacgaggg cggtcgcgag gccgctcgcc cagccgccgg tcgagatgcc
     6241 gccggcgtcg gcgcccacgg cccacacgcc gtcggcgacc cgggcttcgg tgtcgatcgc
     6301 gaggccgccg agcgtcgtcg tgatgccggc gacgacctcg acgaccgtct cgccgtcgcg
     6361 ccgctcgacg aacgtgcccg cggcccgcgc cgcctcgacc atctcgccga cggtgcggtc
     6421 gcgcacgcgc tcgccgagct tctcgtccgg gacgtggaac cacgcgcgcg cccgcggccg
     6481 gcgggcgatc cactgcgcca cgtcgatctc agaccacgtg cgggcgacga actccttccc
     6541 gcgctcgtcg acgatccgcg cgtggcgcgc gtagaactgc gcgagcggcc ggaacgtggc
     6601 cggctcgacg accgcgggcg gcgccgccat cgcgcggccc cagaactcgt ccatcccgtc
     6661 cgagagcgag gcgccggcgg cgagcccgag ccgcagcccg tcgcccgtgc tccacgggtt
     6721 cgagcgcagc gcgaggtgct ccgcctccgg cgtcacgtgc tcgcgcacga ggccgcgacc
     6781 cgccgcgaag ccgccggtcg cgaggacgag cggtgcgccg tccgggagcg c
//
LOCUS       DASTFC010000067         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1187826,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000067 DASTFC010000000
VERSION     DASTFC010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187826"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFO88_01975"
     CDS             complement(<1..614)
                     /locus_tag="VFO88_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9544324.1"
                     /translation="MSAPAAEPATTREERIEAQLAALPSKPGVYLFRGERDAVLYVGK
                     AKSLRPRVRSYFRAGDARRGLERLADRVQRIEVIVTANESEALHLEQNLIRRHRPPFN
                     VRLRDDKSYPYIAVTVGDDYPRVMFTRERHRRGVHYFGPYANAKKVRETLDVLNRVFP
                     YRPCEGPKPGRHSGIPCLDYHIERCLAPCVGYVSKDDYRAVVDGV"
     gene            complement(710..1399)
                     /locus_tag="VFO88_01980"
     CDS             complement(710..1399)
                     /locus_tag="VFO88_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544325.1"
                     /translation="MSDRVARFVGLYATIAAGVAAVVAPLLGLAYFAIPIGAEEETGT
                     VAVWAEPARELAGGLLTWASADRVYASYTQVFALIVPAVLLCAWVTRARRGRAGSRLE
                     RWGWRVSLVAYGMLAAGLAIVALMLIGVSASSTTLDYAYLPLVFPGIVFGTIGSTVLG
                     VGLLRAGYEPRATAWLLALVFPLWIVGDFLIGHNSLGLVPLFLAWATTGWRLFRAGWS
                     AADTREAVTVG"
     gene            1552..2727
                     /locus_tag="VFO88_01985"
     CDS             1552..2727
                     /locus_tag="VFO88_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9544326.1"
                     /translation="MRVRGLLGEATSGRIRRSDLVLPALFGVLGTVEIAVQGYRPLLV
                     ALGTYWLAVLVLCARRLAPLAMPLLVAGIYALTPVLGFDVSEPAAWILLPGLAFFSAG
                     SMVPRSRAPLGLASVVGALLVVFATLDWLTSFDPDLVFGLVWVIGPWMVGVAVRETLE
                     RNRELAVEAERARADAERAAADAAAAERARIARELHDVLAHSLSVMVVQASLAEDLVA
                     RDANGAAAAIREIQQIGRDALGETGRLLRLIRDDGDELGMGPQRSLDDLSALVDDYAR
                     AGLEVELVVDPAARSVPVGVGLSVYRIVQEALTNALKHAPGSCVAVRLERRANVLAVE
                     VENGSGAGEPAVRVESGHGLGGVRERVAIFGGTVDAGPTEGGGFRLVATLPAPAETA"
     gene            2724..3407
                     /locus_tag="VFO88_01990"
     CDS             2724..3407
                     /locus_tag="VFO88_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544327.1"
                     /translation="MTSVVVADDQDLVRSGLRALLEARGIEVLAEAANGREALAAARE
                     CRPDVLVMDIRMPVLDGIAATREVAASGLASRVLVLTTYDLDQYVYEALKAGAAGFML
                     KTSPPDRLAQAVEVVAAGEALLAPSLTRRLIAEHIRRPPPPTGVPEPLRELTARERDV
                     LVLVARGLPNDEIAAELVVSLATVKTHVNRILAKLGLRSRAQAVVAAYESGLVRPGEA
                     PTRDEPAAS"
     gene            complement(3409..>4218)
                     /locus_tag="VFO88_01995"
     CDS             complement(3409..>4218)
                     /locus_tag="VFO88_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET9544328.1"
                     /translation="TVLKPGVVFFDELLPEAAIDRAFALAREAALLLVVGSSLEVHPV
                     AGLPRETLDAGGAVAVVNRDPTWVDPHAALVVRESAGDALAEAVARLRPDDEPVEVVE
                     HDPAWAEAAAAEAARVAGTLDAVAVEHVGSTAVPGLAAKPVLDLLAGLPGDARVSPEQ
                     IRAMEELGYECLGEFGLPGRIFFYKDTDGRRTHHVHAVEHGGDQWQRHVAVRDYLRAH
                     PGEAARYAEVKGAAAARTAGREAYWEAKSAYVEELERRALSASGRPPGPPS"
BASE COUNT          546 a         1563 c         1564 g          545 t
ORIGIN      
        1 accccgtcca cgaccgcgcg gtagtcgtcc ttcgacacgt agccgacgca cggggccagg
       61 cagcgctcga tgtggtagtc gaggcacggg atcccgctgt gccggccggg cttcgggccc
      121 tcgcacggcc ggtacgggaa gacgcggttc aggacgtcga gcgtctcgcg caccttcttc
      181 gcgttcgcgt acgggccgaa gtagtggacc ccgcgccggt gccgctcccg ggtgaacatg
      241 acgcgcgggt agtcgtcgcc cacggtgacc gcgatgtacg ggtacgactt gtcgtcgcgc
      301 aggcggacgt tgaacggcgg ccgatgacgg cggatgaggt tctgctccag gtggagcgcc
      361 tccgactcgt tcgcggtgac gatcacctcg atccgctgga cgcggtcggc gagccgctcg
      421 agcccccggc gggcgtcgcc ggcgcggaag tacgaccgga cgcgcgggcg gagcgacttt
      481 gccttcccga cgtagaggac ggcgtcgcgc tcgccgcgga agaggtagac gccgggcttc
      541 gacggcagcg cggcgagctg cgcctcgatg cgctcctcgc gggtcgtcgc gggctcggcg
      601 gcgggtgcgg acatgcccgt ccagcctagc cccggggcgg gccgccgccg ctcggcggcc
      661 gtttccccgc cccgggtgtc ctgaatgctc ggccccgagc tcccgtgcgt cagccgaccg
      721 tgacggcctc cctcgtgtcg gcggcgctcc agccggcgcg gaagagccgc cagcccgtcg
      781 tcgcccaggc gagaaagagc ggaacgagcc cgaggctgtt gtggccgatc aggaagtcgc
      841 ccacgatcca cagcgggaag acgagcgcca ggagccaggc cgtcgcgcga ggctcgtagc
      901 ctgcgcggag cagcccgacg cccagcaccg tggagccgat cgtcccgaac acgatcccgg
      961 gaaagacgag cgggaggtac gcgtaatcga gggtcgtgct cgacgcggag acgccgatca
     1021 gcatgagcgc cacgatcgcg aggcccgcgg cgagcatccc gtaggcgacg agcgacacac
     1081 gccagcccca gcgctcgagg cgcgatccgg cgcgtccgcg ccgcgcgcga gtcacccacg
     1141 cgcagaggag gacggccggc acgatcagcg cgaacacctg cgtgtaggag gcgtagacgc
     1201 gatccgccga ggcccacgtg agcaagcccc cggcgagctc ccgggcgggc tccgcccaga
     1261 cggcgacggt tccggtctcc tcctcggctc cgatcgggat cgcgaagtac gcgaggccga
     1321 ggaggggagc gacgacggcc gcgacccccg cggcgatcgt cgcatagagg ccgacgaagc
     1381 gagcgacgcg gtcggacatg gcgagtacct ccctggtcga atggcctcgc accgtgcgag
     1441 gcgtcctcat cgtcccgcgc tcgcccggcg atgtcgtcgg gcggcgggtc gatcccgact
     1501 caacccacgg gttgacgacg aacacgcgcg gtcgcgctag cgtgagcggt ggtgagagta
     1561 cgcgggctcc tcggcgaggc cacgtcgggc cgcatcaggc gcagcgacct cgtgctgccc
     1621 gccctcttcg gcgttctcgg cacggtcgag atcgccgtgc aggggtaccg gccgctcctc
     1681 gtggcgctcg ggacctactg gctggccgtc ctcgtgctct gcgcccgtcg cctggccccg
     1741 ctcgccatgc cgcttctcgt cgcggggatc tacgcgctca cgcccgtgct cgggttcgac
     1801 gtcagcgagc ctgcggcctg gatcctcctg cctgggctcg cattcttctc cgccgggagc
     1861 atggtgcccc ggtcgcgtgc cccgctcggc ctcgcgagcg tcgtcggcgc gctcctcgtc
     1921 gtcttcgcga cgctcgactg gctcaccagc ttcgatcccg acctcgtctt cggcctggtc
     1981 tgggtgatcg ggccgtggat ggtcggcgtc gcggtacggg agacgctcga gcggaaccgc
     2041 gagctcgcgg tcgaggccga gcgcgcccgc gccgacgccg agcgcgcggc cgcggacgcg
     2101 gccgccgccg agcgggctcg gatcgcacgc gagctgcacg acgtgctcgc ccactcgctg
     2161 agcgtgatgg tcgtccaggc gtcgctcgcc gaggacctcg tcgcgcgtga cgcgaacggc
     2221 gcggccgccg cgatccgcga aatccagcag atcggccggg acgctctcgg cgagaccggc
     2281 cgtctgctcc gcctgatccg cgacgacggg gacgagctcg gcatgggacc gcagcgctcg
     2341 ctggacgatc tgtcggcgct cgtggacgac tacgcgcggg cgggcctcga ggtcgagctc
     2401 gtcgtcgatc ccgctgcccg ctcggtgccg gtcggcgtcg gcctgtccgt ctaccggatc
     2461 gtgcaggagg cgctgacgaa cgcgctcaag cacgcgccgg gcagctgcgt cgccgtgagg
     2521 ctcgagcgcc gggcgaacgt gctcgcggtc gaggtggaga acggctcggg ggccggcgag
     2581 ccggcggtgc gcgtcgagag cgggcacggt ctcgggggcg tgcgcgagcg cgtcgcgatc
     2641 ttcggcggca ccgtcgacgc cggcccgacc gagggcggcg ggttccggct cgtcgcgacg
     2701 ctaccggcgc ccgccgagac tgcatgacga gcgtcgtcgt cgccgacgac caggatctcg
     2761 tccggagcgg cctccgcgcc ctcctcgagg cccgcgggat cgaggtgctc gcggaggccg
     2821 cgaacggtcg cgaggccttg gcggccgcgc gcgagtgccg cccggacgtg ctcgtgatgg
     2881 acatccgcat gccggtcctc gacggcatcg ccgccacgcg cgaggtggcc gcgagcgggc
     2941 ttgccagcag agttctcgtg ctgacgacgt acgacctcga ccagtacgtc tacgaggcgc
     3001 tgaaggccgg ggcggcgggg ttcatgctca agacgagccc gcccgaccgc cttgcccagg
     3061 cggttgaggt cgtcgcggcc ggcgaggcgc tcctcgcgcc cagcctgacg cggcggctca
     3121 tcgccgagca catccgccgc ccgccgccgc ccaccggcgt ccccgagcct ctccgcgagc
     3181 tgaccgcgcg cgagcgcgac gtactcgtcc tcgtggcccg tggtctcccc aacgacgaga
     3241 tcgccgccga gctcgtcgtg agcctcgcga cggtgaagac ccacgtgaac cggatcctcg
     3301 cgaagctcgg cctgcgcagc cgcgcgcagg cggtcgtcgc cgcctacgag agcgggctcg
     3361 tgcgaccggg cgaggcgccc acccgcgacg agccggccgc gagctgactc acgacggcgg
     3421 acccggcggg cggccagacg ccgagagcgc gcgccgctcg agctcctcga cgtacgcgga
     3481 cttggcctcc cagtacgcct cccggccggc ggtgcgcgcg gcggcggcgc ccttgacctc
     3541 ggcgtagcgc gccgcctcgc cgggatgcgc ccggaggtag tcgcgcacgg ccacgtgtcg
     3601 ctgccactgg tcgccgccgt gctcgacggc gtgcacgtgg tgcgtccggc gcccgtccgt
     3661 gtccttgtag aagaagatcc ggcccgggag cccgaactcg cccaggcact cgtagccgag
     3721 ctcttccatg gcgcggatct gctccgggct gacgcgcgcg tcgcccggta gacccgcgag
     3781 caggtcgagc accggcttcg ccgcgaggcc gggaacggcc gtcgagccga cgtgctcgac
     3841 cgcgacggcg tcgagcgtcc ccgcgacgcg cgcggcctcc gcggcggcgg cctcggccca
     3901 cgccgggtcg tgctcgacga cctcgacggg ctcgtcgtcc ggccggagtc gcgcgaccgc
     3961 ctcggcgagc gcgtcgccgg cgctctcccg cacgacgagc gctgcgtgcg ggtcgaccca
     4021 cgtcgggtca cggttcacga ccgcgacggc gccgcccgcg tcgagcgtct cccgcggaag
     4081 accggcgacc ggatggacct cgagcgacga cccgaccacg aggaggagcg ccgcctcgcg
     4141 cgcgagcgcg aaggcccggt cgatcgccgc ctcggggagc agctcgtcga agaagacgac
     4201 gcccggcttc agcaccgt
//
LOCUS       DASTFC010000068         7146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1039646,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000068 DASTFC010000000
VERSION     DASTFC010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039646"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFO88_02000"
     CDS             complement(<1..903)
                     /locus_tag="VFO88_02000"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9544329.1"
                     /translation="MSAIAAVHGRQILDSRGNPTVEVDVRLESGATGRAAVPSGASTG
                     QFEAVELRDGGDTWRGKGVSQAVANVNGELADALRGLDAGDQRALDRALIELDGTPNK
                     ARLGANAILGCSLAAARAAAADAGVPLYRSVGGEEARVLPVPMMNVVNGGAHAQNRLD
                     FQEFMVVPAGAETFSEALRVGDEVFHALHDLLHERGLATAVGDEGGFAPDLPSTEAAI
                     EAVLEAAERAGHRERIAIALDPAATEVFGDGVYRLPGEGRELDTEGLVGLWAELAERF
                     PIVSIEDGLAEEEWDGWRLLTERLG"
     gene            complement(900..1700)
                     /gene="mazG"
                     /locus_tag="VFO88_02005"
     CDS             complement(900..1700)
                     /gene="mazG"
                     /locus_tag="VFO88_02005"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET9544330.1"
                     /translation="MPEADGPGRALDDLAALAAHLRRECPWDREQTAKTIVPHTVEEA
                     YEVADAALAGDDAKLVDELGDLVFQAVFLALLLEERGEPGLAESIRRVHEKLVRRHPH
                     VFGEAAVGSAAGVKARWEELKVEQEERTGIFHDVPASLPALLLAKKVQRRAATVGFEY
                     PDAAGAFGDLADEVEELRRELPAEDPPGEREPDPRHAGEVGDVLFAAVNVARRLNVDP
                     ELALRAAAGRFRARVEAAERLAAAEGLEFAKLPLAEQDRFYDLAKEAE"
     gene            complement(1703..2806)
                     /locus_tag="VFO88_02010"
     CDS             complement(1703..2806)
                     /locus_tag="VFO88_02010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9544331.1"
                     /translation="MLTKRFSLAVRALAAAAVLTLTLAACGGGDDEPAEVPADAIALV
                     GDTPVPKADFDELMARAQQSYESQERDFPAVGTPEYLDLQSRAVAFLVQRYRFQAEAE
                     ALEIEVTDEEVQERLDQIRDESFEGDQERFDEALEAEGLTVEQAEGEIRAQLLQEKLF
                     ERVTEDVEVTDEDVAAYYEENQEQFTQPASRDVRHILVKNKARADEIHAELENDGGNF
                     AQLARRYSQDTGSKKAGGKLPVQQGSTVPPFDEAAFELDVNEISEPVQTTFGWHIIMP
                     ITEIEPEQVTPLEDVEERIRQQLESERSNAAVETLTQELEKKYPATYAAGFEPAETDV
                     DTTTGETEPPAENGEDTGTGDDTATTEETTTDQ"
     gene            complement(2867..6091)
                     /gene="mfd"
                     /locus_tag="VFO88_02015"
     CDS             complement(2867..6091)
                     /gene="mfd"
                     /locus_tag="VFO88_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET9544332.1"
                     /translation="MDQRTPTTKVSEPPVLHALARELAESERLRAFVDRPGRARVSEP
                     ILALFLAAAWLAREGPLVVLLPDDADVRDAAEAAGWFLGEENVGLFASRGVRRDSGLE
                     PPAHLVGERARALEVLAGGGLVCASALGYAEGLPPAGTRPETIRLAVGDEPGIEALVE
                     RLALAGYARVERVEERGQMAVRGGIVDVFPSTGREPVRIELFGDEIESLRAFSAFTQR
                     ALRTLEETAIHPAAERRLDLVEPHLRDEDDEPLPAPADLVPALPAPDLVWSVGDVREA
                     WAEELLEEPDLGGAIELDQLPSGQPHTFEAQRPAVAARGIAEAERELASFLRGGNRVV
                     VTFAHRGEALRQAGLLRRVDAELLEPGDDLPRADALRFAVAPARRGFVSRELGLVLLP
                     DTQVFRKRPPRTDQRLGRALQSFADLRTGDHVVHEDHGVGQLLGFETKTVAGITRDYL
                     YLGFKGADRLYVPHEQIGKVSRYVGGDSPALSKLGGKAWALMKSRARAGAHELAGELI
                     ALYARRQQAPGVAFDLDGDLLERLEASFPYVETPDQQRAIEAVKEDLEAPRPMDRLVC
                     GDVGFGKTEVAVRAAFAVAEAGRQTLVLAPTTILAQQHWNTFRERYRDLPVTVEMVSR
                     FRKPSEVKRILADYEAGKVDVLVGTHRVLSRDVKAKELGLVVLDEEQRFGVAQKELLR
                     QLRLEVDVLALTATPIPRTLHMSLSGLRDISVIETPPQGRRPVRTHVGEYDEELVQVA
                     LERELARGGQSFVLHNRVETIDEAAEKIRQLVPAARVMVVHGQLPERELEERMLAFLS
                     GDADVLVTTTIIESGLDIPQANTLVVERADALGLAQLYQIRGRVGRSDVTAHAYLFYP
                     DGSELSPEARARLAALADHTELGAGFQIAMRDLEIRGAGDLLGAEQSGHVAAVGFELY
                     LELLGEAVAELSGSRRAVTRPVRVEAQVDAYVPADYIAAEAQKIDLHRRLALVETEDE
                     LRELHAALEDRYGPVPEPVENLFAIQEAKLGLALLGADYLVLRGGKIAVGPLVLGSEE
                     LRELRRSAPTAVYSSARKEVTQRGDGTRACLALLGAILETRLTG"
     gene            6266..6532
                     /locus_tag="VFO88_02020"
     CDS             6266..6532
                     /locus_tag="VFO88_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544333.1"
                     /translation="MPGELVPIGRRPKQVASFAGPGAPARFQAWREANAASLAAVPVE
                     ALRVEHAGFGAARTIRIRIDEDAIPDGLEGPDEAGAGDGLPPAA"
     gene            complement(6538..>7146)
                     /gene="pth"
                     /locus_tag="VFO88_02025"
     CDS             complement(6538..>7146)
                     /gene="pth"
                     /locus_tag="VFO88_02025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9544334.1"
                     /translation="APVRLFRRGERASTLDLLVAGLGNPGREYAATRHNVGFLVADEL
                     ARRYGGSWRSKFSGDLSEVRVDGARVALLKPQTYMNLSGRSVQPAARFFKVEPDALLV
                     VHDEVDLEPGRLQLRAGGGLAGHNGLRSVAQALGSQEFLRLRVGVGRPQRGDPRPVAD
                     FVLDPFDAHEDVDGLVARAADAVEALLAEGLEDAQQRFNERP"
BASE COUNT          892 a         2673 c         2631 g          950 t
ORIGIN      
        1 gccgagccgc tcggtcagga gccgccagcc gtcccactcc tcctcggcga gcccgtcctc
       61 gatcgagacg atcgggaagc gctccgcgag ctccgcccac aggccgacga gcccctcggt
      121 gtcgagctcg cgcccctcgc cggggagccg gtagacgccg tcgccgaaga cctccgtcgc
      181 ggccgggtcg agcgcgatcg cgatgcgctc ccggtgcccg gcgcgctcgg ccgcctcgag
      241 gacggcctcg atcgccgcct cggtggaggg caggtcgggc gcgaagccgc cctcgtctcc
      301 gacggccgtc gcgagcccgc gctcgtgcag gaggtcgtgg agcgcgtgga agacctcgtc
      361 gccgacgcgc agcgcctccg agaaggtctc cgcgcccgcg ggcacgacca tgaactcctg
      421 gaagtcgagc ctgttctggg cgtgggcgcc gccgttcacg acgttcatca tcggaaccgg
      481 aagcacgcgc gcctcctccc cgccgacgga gcggtagagc gggacgcccg cgtcggccgc
      541 cgccgcccgc gccgccgcga gcgagcagcc gaggatcgcg ttcgcgccga gtctcgcctt
      601 gttcggcgtc ccgtccagct cgatcagcgc gcggtcgagc gcgcgctggt cgccggcgtc
      661 gagcccgcgc agcgcgtcgg ccagctcgcc gttcacgttc gcgaccgcct gcgagacgcc
      721 cttcccgcgc cacgtgtcgc cgccgtcgcg gagctcgacc gcctcgaact gcccggtcga
      781 cgccccggac ggcaccgccg cacgcccggt cgcgcccgac tcgaggcgga cgtccacctc
      841 gaccgtcggg ttcccgcggg aatcgaggat ctgccgcccg tgcaccgccg cgatcgcgct
      901 cactcggcct ccttcgccag gtcgtagaac cggtcctgct cggcgagcgg cagcttcgca
      961 aactcgagcc cctcggccgc ggcgagccgc tcggccgcct cgacccgggc ccggaagcgg
     1021 cccgccgccg cccgcagcgc gagctccggg tcgacgttca gccgccgcgc cacgttcacc
     1081 gccgcgaaga ggacgtcgcc gacctcgccg gcgtggcgag gatccggctc gcgctcgccg
     1141 ggaggatcct ccgccggcag ctcgcgccgc agctcttcca cctcgtccgc gaggtcgccg
     1201 aacgcgccgg cggcgtccgg gtactcgaag ccgaccgtcg ccgcgcgccg ctgcaccttc
     1261 ttcgccagga gaagcgccgg cagcgacgcg ggcacgtcgt ggaagatccc cgtgcgctcc
     1321 tcctgctcga ccttgagctc ctcccagcgc gccttcacgc ccgcggccga gccgacggcc
     1381 gcctcgccga agacgtgcgg gtggcggcgg acgagcttct cgtgcacgcg ccggatcgac
     1441 tccgcgagcc ccggctcgcc gcgctcctcg aggaggagcg cgaggaagac cgcctggaag
     1501 acgaggtcgc cgagctcgtc gaccagcttc gcgtcgtccc cggcgagcgc ggcgtcggcg
     1561 acctcgtagg cctcctccac cgtgtgcggg acgatcgtct tcgccgtctg ctcgcggtcc
     1621 cacgggcact cgcggcgcag gtgcgccgcg agtgccgcga gatcgtcgag cgctcggccc
     1681 gggccgtccg cctcgggcac ggctactggt cggtcgtcgt ctcttccgtc gtcgccgtgt
     1741 cgtcgccggt gcccgtgtcc tcgccgttct cggcgggagg ctcggtctca ccggtcgtcg
     1801 tgtcgacgtc ggtctcggcc ggctcgaagc cggcggcgta cgtcgccggg tacttcttct
     1861 cgagctcctg cgtcagcgtc tcgacggcgg cgttcgaccg ctcgctctcg agctgctggc
     1921 ggatccgctc ctcgacgtcc tcgaggggcg tcacctgctc gggctcgatc tcggtgatcg
     1981 gcatgatgat gtgccagccg aaggtcgtct gcaccggctc ggagatctcg ttcacgtcga
     2041 gctcgaacgc ggcctcgtcg aagggcggca ccgtcgagcc ctgctggacc ggaagcttcc
     2101 cgccggcctt cttcgagccc gtgtcctgcg agtagcggcg cgcgagctgg gcgaagttgc
     2161 cgccgtcgtt ctcgagctcc gcgtggatct cgtccgcacg cgccttgttc ttcacgagga
     2221 tgtggcgcac gtcacggctc gccggctgcg tgaactgctc ctggttctcc tcgtagtagg
     2281 ccgcgacgtc ctcgtccgtg acctcgacgt cctcggtcac ccgctcgaag agcttctcct
     2341 gcaggagctg cgcgcggatc tccccctccg cctgctcgac cgtcaggccc tcggcctcga
     2401 gcgcctcgtc gaagcgctcc tggtcgccct cgaacgactc gtcgcggatc tggtcgaggc
     2461 gctcctgcac ctcctcatcg gtcacctcga tctcgagcgc ctccgcctcg gcctggaagc
     2521 ggtagcgctg cacgaggaac gcgacggcgc ggctctggag atccaggtac tcgggcgtgc
     2581 cgacggccgg gaagtcgcgc tcctgcgact cgtagctctg ctgggcgcgg gccatcagct
     2641 cgtcgaagtc ggctttcggg acgggcgtgt cgccgacgag ggcgatcgca tcggccggga
     2701 cctcggccgg ctcgtcgtcc ccgccgccac acgcggcgag tgtcagcgtc agcacggccg
     2761 cggctgcgag cgcgcggacg gcgagggaaa agcgcttggt cagcatggga acgagtacct
     2821 gcctaacgaa tgaggaagtg acacgggtca gggggaaccg gccgtcctag ccggtcagcc
     2881 gagtttcgag gatagcaccg agtaacgcga ggcaggcccg tgtaccgtcc ccccgctgcg
     2941 tgacctcctt gcgggccgac gagtacacgg cggtgggcgc gctccggcgc agctcgcgca
     3001 gctcctccga cccgaggacg agcggcccga cggcgatctt cccgccgcgc aggacgaggt
     3061 agtcggcccc gaggagcgcg agcccgagct tggcctcctg gatcgcgaag aggttctcga
     3121 ccggctccgg cacgggcccg tagcggtcct cgagcgccgc gtggagctcg cgcagctcgt
     3181 cctccgtctc gacgagcgcg agccggcggt ggaggtcgat cttctgcgcc tcggccgcga
     3241 tgtagtccgc ggggacgtag gcgtcgacct gcgcctccac ccggacgggc cgggtgaccg
     3301 cccggcgcga gccggagagc tccgccacgg cctcgccgag cagctcgagg tagagctcga
     3361 acccgaccgc cgcgacgtgg ccggactgct cggcgccgag caggtcgccg gcgccgcgga
     3421 tctcgaggtc gcgcatggcg atctggaagc cggcgcccag ctccgtgtgg tcggcgagcg
     3481 cggcgagcct ggcgcgtgcc tcgggcgaga gctccgagcc gtcgggatag aagaggtacg
     3541 cgtgcgcggt cacgtcgctc cggccgacgc gcccgcggat ctggtagagc tgggcgagcc
     3601 cgagcgcgtc cgcgcgctcg acgacgagcg tgttcgcctg cgggatgtcg agcccggact
     3661 cgatgatcgt cgtcgtcacg agcacgtccg cgtcgccgga gaggaacgcg agcatccgct
     3721 cctcgagctc ccgctccggg agctgcccgt gcacgaccat cacgcgggcc gccgggacga
     3781 gctgccggat cttctccgcc gcttcgtcga tcgtctcgac gcggttgtgg agcacgaacg
     3841 actgcccccc gcgggcgagc tcccgctcca gggcgacctg gacgagctcc tcgtcgtact
     3901 cgccgacgtg cgtccgcacc ggccggcggc cctggggcgg cgtctcgatc acgctgatgt
     3961 cacgcaggcc ggagagcgac atgtgcaggg tccgcgggat cggcgtcgcg gtgagcgcga
     4021 gcacatctac ctcgagccgg agctggcgca ggagctcctt ctgcgcgacg ccgaagcgct
     4081 gctcctcgtc gaggacgacg aggccgagct ccttcgcctt cacgtcgcgc gagaggacac
     4141 ggtgcgtgcc gacgaggacg tcgaccttcc cggcctcgta gtccgccagg atccgcttga
     4201 cctccgacgg cttgcggaag cgcgacacca tctcgaccgt gaccgggagg tcgcggtagc
     4261 gctcgcggaa cgtgttccag tgctgctgcg cgaggatcgt cgtcggggcg aggacgagcg
     4321 tctgccgccc ggcctcggcg accgcgaacg cggcacgcac cgcgacctcc gtcttcccga
     4381 agccgacgtc gccgcagacg agccggtcca tcgggcgcgg cgcctcgagg tcctccttca
     4441 cggcctcgat cgcgcgctgc tggtcgggcg tctcgacgta cgggaagctc gcctcgaggc
     4501 gctcgagcag gtcgccgtcc aggtcgaagg cgacgccggg cgcctgctgg cggcgcgcgt
     4561 agagggcgat cagctcgccg gcgagctcgt gcgcgcccgc gcgggcgcgc gacttcatga
     4621 gcgcccaggc cttcccgccg agcttcgaga gggcgggcga gtcgccgccg acgtaccggg
     4681 agaccttccc gatctgctcg tgcgggacgt agagccggtc ggcgcccttg aagccgaggt
     4741 agaggtagtc gcgggtgatc cccgcgaccg tcttcgtctc gaagccgagc agctgcccga
     4801 cgccgtggtc ctcgtggacg acgtggtcgc cggtgcgcag gtcggcgaag ctctggagcg
     4861 cgcggccgag gcgctggtcg gtgcgcggcg ggcgcttgcg gaagacctgc gtgtccggga
     4921 ggaggacgag cccgagctcg cgcgagacga acccgcggcg ggcgggcgcg acggcgaagc
     4981 ggagggcgtc cgcccgcggg aggtcgtcgc ccggctcgag gagctcggcg tcgaccctgc
     5041 gcaggaggcc ggcctggcga agcgcctcgc cgcgatgggc gaacgtgacg acgacgcggt
     5101 tcccgccgcg gaggaaggac gcgagctcgc gctcggcctc cgcgatcccg cgcgccgcga
     5161 cggccgggcg ctgcgcctcg aacgtgtgcg gctggccgga cgggagctgg tccagctcga
     5221 tcgccccgcc gaggtcgggc tcctcgagga gctcctccgc ccaggcctcg cgcacgtcgc
     5281 cgacgctcca gacgaggtcg ggcgccggca gggcgggaac gaggtcggcc ggggccggga
     5341 gcggctcgtc gtcctcgtcg cgcaggtgcg gctcgacgag atcgaggcgg cgctcggcgg
     5401 ccgggtggat cgccgtctcc tcgagcgtgc ggagcgcgcg ctgcgtgaag gcggagaacg
     5461 cgcggagcga ctcgatctcg tcgccgaaga gctcgatccg gaccggctcg cggccggtcg
     5521 aggggaagac gtcgacgatc ccgccgcgga cggccatctg gccgcgctcc tcgacccgct
     5581 cgacgcgtgc gtagccggcg agcgcgagcc gctcgacgag cgcctcgatg cccggctcgt
     5641 cgccgaccgc gagccggatc gtctccggcc gcgtccccgc tgggggcagg ccctccgcgt
     5701 acccgagcgc cgacgcgcag acgaggccgc cgcccgcgag cacctcgagc gcgcgcgccc
     5761 gctcgccgac gagatgcgcc ggcggctcga gcccggaatc cctgcggacg ccgcgcgagg
     5821 cgaagaggcc gacgttctcc tcgccgagga accagcccgc cgcctcggcc gcgtcgcgca
     5881 cgtcggcgtc gtccgggagg aggacgacga gcggcccctc ccgcgcgagc cacgcggcgg
     5941 cgaggaagag cgcgaggatc ggctccgaga cgcgcgcgcg gcccggccgg tcgacgaacg
     6001 cccgcaggcg ctcgctctcg gcgagctcgc gcgcgagcgc gtggaggacg ggcggctccg
     6061 agaccttcgt tgtcggcgtg cgctggtcca tgacgacggg cggaacacgc ggttccgccg
     6121 gccgcgaatc gtagccgggc ggcggctcgc cgcggccgcg tggacgcggg cgcccgccga
     6181 ggccctcgct agtaacacag tgttaccagc gagcgggctc ccgcccgctt gggcatcgcc
     6241 gggcgacggc ttagactcgc gcgcgatgcc cggtgagctc gtcccgatcg gccggcggcc
     6301 gaagcaggtc gcgtccttcg cgggccccgg cgcgcccgcg cggttccagg cgtggcgcga
     6361 ggcgaacgct gcctcgctcg cggcggtgcc cgtcgaggcg ctccgcgtcg agcacgcggg
     6421 cttcggcgcg gcccggacga tcaggatccg gatcgacgag gacgcgatcc cggacgggct
     6481 cgagggccct gacgaggcgg gcgccggcga cggcctcccg cccgcggcgt aacgaggcta
     6541 cgggcgctcg ttgaagcgct gctgcgcgtc ttcgagcccc tcggcgagga gcgcctcgac
     6601 ggcgtccgcc gcccgggcga cgaggccgtc gacgtcctcg tgcgcgtcga aggggtcgag
     6661 cacgaagtcg gcgacgggcc gcggatcgcc gcgctgcggc ctcccgacgc cgacgcggag
     6721 ccgcaggaac tcctgcgagc cgagcgcctg ggcgaccgag cggagcccgt tgtgccccgc
     6781 gagcccgccc cccgcgcgga gctggaggcg ccccggctcg aggtcgacct cgtcgtggac
     6841 gacgagcagg gcgtcgggct cgaccttgaa gaaccgcgcc gccggctgca cggagcggcc
     6901 ggagaggttc atgtacgtct gcggcttgag gagcgcgaca cgggcgccgt cgacccgcac
     6961 ctccgagagg tcgcccgaga acttcgagcg ccacgagccg ccgtaccggc gcgcgagctc
     7021 gtccgcgacg aggaagccga cgttgtggcg cgtcgccgcg tactcgcgcc cggggttccc
     7081 gagccccgcc acgagcaggt cgagcgtcga ggcccgctcg ccccgacgga acaaccgcac
     7141 aggcgc
//
LOCUS       DASTFC010000069         6888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1550477,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000069 DASTFC010000000
VERSION     DASTFC010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550477"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFO88_02030"
     CDS             <1..443
                     /locus_tag="VFO88_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9544335.1"
                     /translation="RPARFLAITRHPYAVCEGMRRRSKATIEDAARQWATANEVMLAD
                     AERLEHFLLLSYEELTERTDETLERMRAFLGLEQPFDRSVAERPLRMVNVEGEPRPLQ
                     NFNVKSLERLTDEEIATIDRIAGPMMERLGYVPLERPAHAGAGA"
     gene            440..1561
                     /locus_tag="VFO88_02035"
     CDS             440..1561
                     /locus_tag="VFO88_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9544336.1"
                     /translation="MTGERSLRILAVGDARSIHTIRWARRLDERGHTMHLVTDRMPRP
                     NDDLGGVVVHDVRRLGLITRVRGLRKPFIGRTIGRLARHLAVDVVHAHGLLPYAWWAA
                     QGGFHPLVVSPWGRDALLDAQTEPGRSRARLAFAEADHLVVNSQAILDASIPLGADPD
                     RTLHTIWHTRLDGFSPDRADRARLRRELGWPEDALVVLSLRNFQARTNIDVLVRAFDR
                     ARREVPEARLLLAARGGETKAEIERLVEELALGDLVRFHRVEPDELPPLAASADLTVS
                     IADTDSTPSSLLEAMASGQPMIGGWCASIDEWIQPGEGAEMIEPKDEDALVEALLKLL
                     RDPELRRRYGNRNAEITRQRVAESAPAMEKLYRELAAAR"
     gene            1589..3133
                     /locus_tag="VFO88_02040"
     CDS             1589..3133
                     /locus_tag="VFO88_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET9544337.1"
                     /translation="MASDTPSSAPPRRSPGRLSQLAGQSVIYGLGGTLARLAGVFLVP
                     VYLAAAGTEAFGEAELVVSAVAVAAIALRLGIVNSMSRFTLAESPTGDWRPVIHTIYG
                     FVLAASTTAAVVGLLARAQIADALQVSETLAVAGVAGLWIVMNYDVLARVYRIERRPK
                     AFVAFQLTNVAITVVLTIILVVVYDYGAVGLLVGNFAGTAVVFLAMLWARRSTVGVTR
                     FERPLLGELLRYSIPLMPTNLAIWALNFADRLQVQRLASPEELGEYAAAAKVAAGMTL
                     FLAAFQSAWTPFAHALRGEEGDEVAKRTYAEVFTFWSVVMGWGLVALTVASAPYIELT
                     FPEGAREDAIPVVPLLATGVVLYGIYLIVNIGVTISKRTQWTPFIAGFAGAVTVGLNF
                     LFIPAFGIVGAGITTVIGFALLAGLQWANARRMWRVDYEWSRVARIAVVAAVIVALSL
                     WVVPVTGVLGLVGRAVLIAVYPLGLLALRAVSRDDLRRIPGALRAARRRRGRRGDEDD
                     AADVTP"
     gene            complement(3123..3998)
                     /locus_tag="VFO88_02045"
     CDS             complement(3123..3998)
                     /locus_tag="VFO88_02045"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9544338.1"
                     /translation="MPPSDHKFVFVCGLQRSGTTMLYRYLDEHPEVSALSGTARDTNE
                     GQHNQTVYPGDEWHGKGGRFAFRPEARFTEDSPLVTPENAEKLFEEWSRFWDTSRPYL
                     LEKSPPNLIRMRFLQALFPESSFVVLLRHPIPVSLATQRWGGDRMHRLVEHWLRAHEL
                     FVEDLPHLRRVHTVRYEDLVADPDGVLGRAFAFLGLKDPAAGRRERAEGVNADNFQAD
                     RTLRTGVNDRYFAEWRHRKRGLVPRAYYAAIERRYERRVRGFGYSLRRPEELREPELA
                     LPGLAAEAAPAPVTA"
     gene            4056..4925
                     /locus_tag="VFO88_02050"
     CDS             4056..4925
                     /locus_tag="VFO88_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET9544339.1"
                     /translation="MQPTAPARWTLAKRAEMRWRGATRRARALPDFLVLSAARCGSTS
                     LFAALSEHPQVLPPSHKETHFFDRNHERGLDFYRRLFPLEAHRRLRARGRGGPVLTGE
                     ATTYYLLHPAVPARAHAAVPEARLVLILRDPVDRAYSHYQLSVRRGKEELSFEEALDA
                     EPERLAGAEERLLADPAADVPSHRLHSYVTRGLYLEQLLRWEELYGRERILFVRSEDF
                     FADPVGAVGRVTGFLGLRPHPGPLPPAKNVASYDTMKPETRSRLREIFAEPNRRLEEH
                     LGRELGWQTPGDS"
     gene            complement(5102..5707)
                     /locus_tag="VFO88_02055"
     CDS             complement(5102..5707)
                     /locus_tag="VFO88_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9544340.1"
                     /translation="MAVEVGIDVETLRFEIRKTYARVSDEPESGFIFPTGRAWALDLD
                     YPRELLDRVPDRSAESFAGVANPFALGALEPGEVVLDLGAGAGTDSLVAAQMVAPGGR
                     VTGIDMTPEMVAKAQASAAEAGLDNVEFLSGDAESLPFEDERFDVVISNGVIDLVPDK
                     DAVFSEVFRVLRPGGRIQIADVTIQSPVSEEGRRKIDLWTG"
     gene            complement(5741..>6888)
                     /locus_tag="VFO88_02060"
     CDS             complement(5741..>6888)
                     /locus_tag="VFO88_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544341.1"
                     /translation="LVGERDFGVESRTALLSFVVAFGTAKAIANLVAGGAADRVGRRR
                     LLVAGWLLAAPTPLLIALAPSWEYVVAANVFLGASQGLAWSMTVVMKIDLAGPRRRGL
                     ALGLNESAGYVGVALAAFASGVVAASVAPQTVVWAGSLGVVVLGTAVSLLLVRDTAAH
                     VALEQGNRRATIGLAHAFRRGTATHPVLRACSQAGLVNNANDALAWGLVPLYLAAHGA
                     GTARIGVVAAVYPAVWGVGQLAAGWASDHLGRKPLIVSGMLVQAVALALLVQGGGAFG
                     PSLVAAVLLGAGTALAYPTLIAAVSDAVEPRERAPAVGVYRFWRDAGFVLGALAAGAA
                     ADAFGASTAIALVAGLTAASGAWVGATRWTDGGRLSAKPMGDIATGI"
BASE COUNT          895 a         2599 c         2485 g          909 t
ORIGIN      
        1 tccgcccggc gcgcttcctc gcgatcacgc ggcacccgta cgccgtctgc gaggggatgc
       61 gccgccgctc gaaggcgacg atcgaggacg cggcgcgcca gtgggcgacg gcgaacgagg
      121 tgatgctcgc cgacgcggag aggctcgagc acttcctgct cctctcgtac gaggagctga
      181 ccgagcgcac cgacgagacg ctcgagcgca tgcgagcctt cctcgggctc gagcagccgt
      241 tcgaccgctc cgtcgccgag cgcccgctgc ggatggtgaa cgtcgagggc gagccgcggc
      301 cgctccagaa cttcaacgtg aagagcctcg agcggctgac cgacgaggag atcgcgacga
      361 tcgaccggat cgccggcccc atgatggagc gcctcggcta cgtcccgctc gagcggccgg
      421 cgcacgcggg cgcgggcgcg tgacgggcga gcggtcgctg cgcatcctcg cggtcgggga
      481 cgcgcgctcg atccacacga tccgctgggc ccgccgcctc gacgagcgcg ggcacacgat
      541 gcacctcgtg accgaccgaa tgccgcgccc caacgacgac ctcggcggcg tggtcgtcca
      601 cgacgtccgc cggctcgggc tgatcacccg cgtgcgcggc ctgcggaagc ccttcatcgg
      661 gcggacgatc ggccggctcg cgcggcacct cgccgtcgac gtcgtccacg cccacggcct
      721 gcttccgtac gcgtggtggg cggcgcaggg cggcttccac ccgctcgtcg tctcgccgtg
      781 gggccgcgac gcgctcctcg acgcccagac ggagccgggc cggagccgcg cccgtctcgc
      841 gttcgcggag gccgaccacc tcgtcgtcaa ctcgcaggcg atcctcgacg cgtcgatccc
      901 cctcggcgcc gacccggatc gcacgctgca cacgatctgg cacacgcgcc tggacggctt
      961 ctcgcccgac cgggccgacc gcgcccggct gcgccgcgag ctcggctggc cggaggacgc
     1021 gctcgtcgtc ctctccctac gcaacttcca ggcgcggacg aacatcgacg tcctcgtgcg
     1081 cgcgttcgac cgcgcgcggc gcgaggtgcc cgaggcgcgg ctcctgctcg ccgcgcgcgg
     1141 cggcgagacg aaggccgaga tcgagcggct cgtcgaggag ctcgcgctcg gcgacctcgt
     1201 ccgcttccac cgcgtcgagc cggacgagct cccgcccctc gccgcctcgg ccgacctgac
     1261 cgtgtcgatc gcggacacgg actcgacccc gtcctcgctc ctcgaggcga tggcgagcgg
     1321 ccagccgatg atcggcggct ggtgcgcgtc gatcgacgag tggatccagc cgggcgaggg
     1381 ggccgagatg atcgagccca aggacgagga cgccctcgtc gaggccctcc tgaagctcct
     1441 ccgcgacccc gagctgcgcc ggcgctacgg gaaccggaac gccgagatca cgcggcagcg
     1501 cgtggccgag tcggcgccgg cgatggagaa gctctaccgc gagctcgcgg ccgcccgctg
     1561 agccgcacgg caggattcgc cgcagcccgt ggcgtccgac acccctagct ccgcgccgcc
     1621 ccgccgcagt cccggccggc tgagccagct cgcgggacag tcggtcatct acggcctcgg
     1681 cgggacgctc gcgcggctcg cgggcgtctt cctcgtccct gtctacctcg ccgcggccgg
     1741 gaccgaggcg ttcggcgagg ccgagctcgt cgtctcggcc gtcgcggtcg cggcgatcgc
     1801 gctgcggctc gggatcgtca actcgatgtc ccgcttcacc ctcgccgaga gcccgacggg
     1861 cgactggcgg ccggtcatcc acacgatcta cgggttcgtc ctcgccgcgt cgacgacggc
     1921 cgcggtcgtc gggctcctcg cgcgggcgca gatcgcggac gcgctccagg tgagcgagac
     1981 cctcgcggtc gcgggcgtcg cggggctctg gatcgtcatg aactacgacg tcctcgcccg
     2041 cgtctaccgc atcgagcggc ggccgaaggc gttcgtcgcc ttccagctca cgaacgtcgc
     2101 gatcacggtc gtgctgacga tcatcctcgt cgtcgtctac gactacggcg cggtcgggct
     2161 gctcgtcggg aacttcgcgg ggacagcggt cgtcttcctc gccatgctct gggcgcggcg
     2221 gagcacggtc ggggtcacgc gcttcgagcg gccgctcctc ggcgagctcc tgcgctactc
     2281 gatcccgctc atgccgacga acctcgcgat ctgggcgctc aacttcgccg accgcctcca
     2341 ggtgcagcgg ctcgcgagcc cggaggagct cggcgagtac gcggccgccg ccaaggtggc
     2401 cgcggggatg acgctcttcc tcgccgcgtt ccagtccgcg tggacgccgt tcgcgcacgc
     2461 cctccgcggc gaggagggag acgaggtggc gaagcggacg tacgcggagg tcttcacgtt
     2521 ctggtcggtc gtgatgggct ggggcctcgt cgcgctcacg gtcgcctccg cgccgtacat
     2581 cgagctcacc ttccccgagg gcgcgcgcga ggacgcgatc ccggtcgtgc cgcttctcgc
     2641 gaccggcgtc gtcctgtacg ggatctacct gatcgtgaac atcggcgtca cgatctcgaa
     2701 gcgcacccag tggacgccgt tcatcgccgg tttcgcgggc gcggtcaccg tcgggctcaa
     2761 cttcctcttc atccccgcgt tcgggatcgt cggagccggg atcacgaccg tgatcgggtt
     2821 cgcgctcctc gccgggctcc agtgggcgaa cgcgcggcgg atgtggcgcg tggactacga
     2881 gtggagccgc gtcgcgcgga tcgccgtcgt cgccgcggtg atcgtcgcgc tctcgctctg
     2941 ggtcgtcccc gtgacgggcg tgctcgggct cgtcggccgc gccgtgctca tcgcggtcta
     3001 tccgctcggg ctcctggcgc tccgggccgt gtcgcgcgac gacctgcggc ggattcccgg
     3061 cgcgctcagg gctgcgaggc ggcggcgcgg gcggcgcggc gacgaggacg acgccgccga
     3121 cgtcacgccg tgacgggggc gggcgccgcc tcggcggcga gcccggggag cgcgagctcc
     3181 ggctcgcgga gctcctccgg ccggcggagc gagtagccga acccgcgaac gcgccgctcg
     3241 tagcgccgct cgatcgccgc gtagtacgcg cgcggcacga gcccgcgctt ccggtgccgc
     3301 cactcggcga agtacctgtc gttcacgccg gtgcggagcg tccggtcggc ctggaagttg
     3361 tccgcgttca cgccctcggc gcgctcgcgc cggccggcgg ccggatcctt gaggccgagg
     3421 aacgcgaacg cgcgcccgag cacgccgtcc ggatcggcga cgaggtcctc gtagcgcacc
     3481 gtgtgcacgc ggcgcaggtg cgggaggtcc tcgacgaaga gctcgtgcgc gcggagccag
     3541 tgctccacga gccggtgcat ccggtcgccg ccccagcgct gcgtcgcgag cgagaccggg
     3601 atcgggtgcc ggagcaggac gacgaacgag gactccggaa agagcgcctg gaggaagcgc
     3661 atccggatga ggttcggcgg cgacttctcg aggaggtacg gcctcgacgt gtcccagaag
     3721 cggctccact cctcgaagag cttctccgcg ttctccggcg tgacgagcgg cgagtcctcc
     3781 gtgaagcgcg cctcggggcg gaacgcgaac cggccgccct tcccgtgcca ctcgtcgccc
     3841 ggatagacgg tctggttgtg ctggccctcg ttcgtgtcgc gcgccgtgcc ggagagggcg
     3901 ctcacctcgg ggtgctcgtc gaggtagcgg tagagcatcg tcgtgccgct ccgctggagg
     3961 ccgcagacga acacgaactt gtgatccgag ggaggcatgg ccagcggcga tcgtaccggc
     4021 gggcgggcgg gatcgcgccg gtatgctcgg ccgccgtgca gccgaccgcc cccgcccgct
     4081 ggacgctcgc gaagcgcgcc gagatgcgct ggcgcggcgc gacccggcgc gcgcgggcgc
     4141 tgccggactt cctcgtcctg agcgcggccc gctgcgggtc gacctcgctc ttcgccgcgc
     4201 tctcggagca cccgcaggtg ctgccgccga gccacaagga gacgcacttc ttcgaccgca
     4261 accacgagcg cgggctcgac ttctaccggc ggctcttccc gctcgaggcg caccggcggc
     4321 tccgggcgcg gggccgcgga ggccccgtgc tgacgggcga ggcgacgacg tactacctcc
     4381 tccacccggc cgtgccggcg cgcgcccacg cggcggtccc ggaggcgcgg ctcgtcctca
     4441 tcctgcgcga ccccgtcgac cgggcgtact cgcattacca gctctcggtg cgccgcggga
     4501 aggaggagct ctcgttcgag gaggcgctcg acgcggagcc cgagcggctc gcgggcgcgg
     4561 aggagcggct gctcgccgac ccggccgccg acgtgccgtc ccatcgcctc cattcgtacg
     4621 tcacgcgcgg gctgtacctc gagcagctgc tccgctggga ggagctctac gggcgcgagc
     4681 ggatcctctt cgtccggagc gaggacttct tcgccgaccc ggtcggcgcg gtcgggcggg
     4741 tgacgggctt cctcggcctg cgcccgcacc cgggcccgct cccgcccgcg aagaacgtcg
     4801 cgtcgtacga cacgatgaag ccggagacgc ggtcgcgcct gcgcgagatc ttcgccgagc
     4861 cgaaccgccg cctcgaggag cacctgggcc gcgagctcgg ctggcagacg ccgggcgact
     4921 cctaacgagg cctgtacgcc ttcacggtcg tcccgcgggc gccgtacttc tcggccttgc
     4981 ggcgcgtgtc ctcgaacgcc gagctcgcgt aggtctcgac gaggtcgccg gtcgcgatct
     5041 cccggaagcc gtgccgctcg agcgtcctcg cgtactctgc ctccagcaga gcgccggcga
     5101 ttcaccccgt ccagaggtcg atcttccggc gcccctcctc ggacaccggg ctctggatcg
     5161 tcacgtcggc gatctggatg cgcccgcccg gtcgcagcac gcggaagacc tccgagaaga
     5221 ccgcgtcctt gtccgggacg aggtcgatga cgccgttcga gatgacgacg tcgaagcgct
     5281 cgtcctcgaa cggcaggctc tccgcgtcgc ccgagaggaa ctcgacgttg tcgagcccgg
     5341 cctccgcggc gctcgcctgc gccttcgcga ccatctccgg ggtcatgtcg atccccgtca
     5401 cgcggccgcc gggcgcgacc atctgcgcgg cgacgagcga gtcggtgccc gcgccggcgc
     5461 cgaggtccag cacgacctcg cccggctcga gcgcgccgag cgcgaacggg ttcgcgaccc
     5521 cggcgaacga ctcggccgag cggtcgggga cgcggtcgag gagctcgcgc gggtagtcga
     5581 gatcgagcgc ccacgcgcgc cccgtcggga agatgaagcc gctctccggc tcgtcggaga
     5641 cccgcgcata cgtcttgcgg atctcgaagc ggagcgtctc gacgtcgatg ccaacctcga
     5701 ccgccatcga ccgaccctaa ccggcgaaga acgcggccgg ctagatccct gtggcgatgt
     5761 cacccatcgg tttcgccgat aggcggccgc cgtccgtcca ccgcgtggcg ccgacccatg
     5821 cgccgctcgc cgcggtgagg ccggcgacga gcgcgatcgc ggtcgacgcg ccgaacgcgt
     5881 ccgccgcggc gccggcggcg agcgcgccca ggacgaagcc cgcgtcgcgc cagaagcggt
     5941 acacgccgac cgcgggcgcc cgttcgcgcg gctcgacggc gtccgagacg gccgcgatga
     6001 gcgtcgggta ggcgagcgcc gtccccgcgc ccagcagcac tgcggccacg agcgacgggc
     6061 cgaacgcgcc gcctccctgg acgaggaggg caagcgccac cgcctgcacg agcatcccgg
     6121 agacgatcag cggcttgcgt cccagatggt cggaggccca gccggcggcg agctgtccga
     6181 cgccccacac ggccgggtag acggcggcga ccaccccgat ccgcgcggtg cccgccccgt
     6241 gcgcggcgag gtacagcgga acgaggcccc aggcgagcgc gtcgttggcg ttgttcacga
     6301 gcccggcctg cgagcacgcg cgcagcacag ggtgcgtcgc cgtgccgcgg cggaacgcgt
     6361 gcgcgagccc gatcgtcgcc cgccggttcc cctgctcgag cgcgacgtgc gcggccgtgt
     6421 cccgcacgag gaggagggac accgccgtcc cgagcacgac gacgccgagc gagccggccc
     6481 agacgaccgt ctgcggcgcg acgctcgccg cgaccacgcc cgaggcgaac gcggccagcg
     6541 ccacgccgac gtacccggcc gactcgttca gcccgagcgc caggccgcgc cgccgggggc
     6601 cggccaggtc gatcttcatc acgaccgtca tcgaccacgc gagaccctgg ctcgcgccga
     6661 ggaagacgtt cgcggccacg acgtattccc acgacggcgc gagcgcgatg aggagcggcg
     6721 tcggcgcggc gaggagccag ccggcgacga gcagccgccg ccggcccacc cggtcggctg
     6781 cgccgcccgc gacgaggttc gcgatcgcct tcgccgtccc gaacgcgacg acgaaggaga
     6841 ggagcgccgt gcgtgactcg acgccgaagt cgcgctcgcc gacgagcg
//
LOCUS       DASTFC010000070         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_100196,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000070 DASTFC010000000
VERSION     DASTFC010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100196"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFO88_02065"
     CDS             complement(<1..1073)
                     /locus_tag="VFO88_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET9544342.1"
                     /translation="MTASRSPCPRHGATARASVLRRTGRAATILVAALATLLVATPAG
                     ADPASEKQAEAEAVLAQIEALDMEVGKAVEEYNGARVMLERIHEQQEENQRALKIAKQ
                     SFGRAQDALEVRIHQLYTSGDSSTLEILLGATSIEDFLARVDATSRVSEQDARIVDEI
                     RAVRGEVKEREAELEAAREEQAAIVAELEQRKAAIEAQLAEREAMLATIRDEIERIQA
                     EERRRQARLQAQAEERLAEEESAPAPVSSGGSGGGGGGNPPPSQYGGVVGVAMQFLGV
                     PYVWGGASPSGFDCSGLVMYAFAQVGVSLPHNAAMQYAYGSPVSKSALQAGDVVFFNG
                     LGHNGIYIGGGQFVHAPHTGDVV"
     gene            1179..2216
                     /locus_tag="VFO88_02070"
     CDS             1179..2216
                     /locus_tag="VFO88_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HET9544343.1"
                     /translation="MRAAARRHARLARLAVSVFAATATLLGAAGAHADQAGEAARDAQ
                     GFADAGDRLAAEAQAVTLELYALESELAAARERLASLQARAAAVEEEREAARTHLAAV
                     RATLATAEERLAARLRELYVQSDPDPLAVLLGAQSLDEALTALDSLGRFADQDRTVLE
                     QVRAARAAAHDAVERLAAREAELEALVADAADTEASLAASQESRGAYLAGLREEQRLN
                     AAQLARAEEQAAAAQQKAEAIPAAQPSVAAPAPVGGPVAGPGRQLTVEVTAYALPGTT
                     ATGIPVGWGVVAVDPSVIPLGTRMTIPGYGEGVAADTGSAVKGHVIDVWMPTREQALA
                     WGRRTLTITLH"
     gene            2316..2969
                     /locus_tag="VFO88_02075"
     CDS             2316..2969
                     /locus_tag="VFO88_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544344.1"
                     /translation="MNEQPRVLLVDDHDLFRTGLAKLLTEQGVHVIGEAPTGEAALRL
                     VDELAPDVVIMDLNMPGLGGVDATREISASAPRTRVVVLTISEDDDDIVDAIMAGACG
                     YLLKNSTVQELVAGISAAADGDSLISPQVGTKVLQQLRAQAAGVRRDTAAPRIQLSER
                     ELDVLRLIAIGKDNAQIAQELFISPKTVKNHISNILMKLQIENRIQAAVYAVRSGIV"
     gene            complement(2966..3211)
                     /locus_tag="VFO88_02080"
     CDS             complement(2966..3211)
                     /locus_tag="VFO88_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544345.1"
                     /translation="MEHTLTDGYAEALAIEGERLRTEREIAAGLAEAGGEAGAAHLVA
                     LSERLAELELELTRLRASLDGLRARRESVLRGAAERL"
     gene            3374..4585
                     /gene="dacB"
                     /locus_tag="VFO88_02085"
     CDS             3374..4585
                     /gene="dacB"
                     /locus_tag="VFO88_02085"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET9544346.1"
                     /translation="MSTARTRLLLALAVVLSVAVSAPAAAAQSLATRLDRALNTSGVS
                     WSASGAIALNLSNGRVAYARNLDKPFEPASNQKLTVTLAALHKLGPDYRIPTQAYGEG
                     RQDGDVWRGRIVIKGFGDPTLTTSDLNTLARKVRDLGITRVTGGVRGDETYFDTRRTA
                     PGWKPSFYKVWCPPLSALVVDRARVNGTVVDRPALAAAHAFRAALVRNGVAVMRSARV
                     GPLGEEAVALARVRSPRIALLVRRMNRPSDNFYAEMLLKKLGAHFRGKGTSGAGATVV
                     RRVFRARGVPLAGIRIADGSGLSSLDRLTTRAISRMLVSARKDPVIGLPFFNSLPIAG
                     VNGTLKDRMRRGPARGVVHAKTGTTLAASALSGYVGSRWVFSILQNGSPVPWTSSRAS
                     QDRFAQILAGG"
BASE COUNT          639 a         1840 c         1659 g          619 t
ORIGIN      
        1 ttcacaacgt cgcccgtgtg cggcgcgtgg acgaactggc cgccgccgat gtagatcccg
       61 ttgtggccga gcccgttgaa gaagacgacg tcgccggcct ggagcgccga cttcgagacc
      121 ggggagccgt acgcgtactg catcgcggcg ttgtgcggaa gcgacacgcc cacctgcgcg
      181 aacgcgtaca tgacgagccc ggagcagtcg aagccgctcg gcgacgcgcc gccccagacg
      241 taggggacac cgaggaactg catcgccacg ccgacgacgc cgccgtactg cgagggcggc
      301 gggttcccac caccgccgcc accgctgccg ccgctcgaga cgggcgccgg cgcgctttcc
      361 tcttcggcga gccgctcctc cgcctgcgcc tggagccggg cctggcggcg ccgctcctcg
      421 gcctggatcc gctcgatctc gtcacggatc gtcgcgagca tcgcctcgcg ctcagcgagc
      481 tgcgcctcga tcgcggcctt gcgctgctcg agctcggcga cgatcgccgc ctgctcctcg
      541 cgcgccgcct cgagctcggc ctcgcgctcc ttgacctcgc cgcgcacggc gcggatctcg
      601 tcgacgatgc gggcgtcctg ctccgagacg cgactggtcg cgtccacgcg cgcgaggaag
      661 tcctcgatgc tcgtcgcgcc gagcaggatc tcgagcgtcg acgagtcgcc cgacgtgtac
      721 agctggtgga tccgcacctc gagcgcgtcc tgggcccggc cgaagctctg cttcgcgatc
      781 ttgagcgccc gctggttctc ctcctgctgc tcgtggatcc gctcgagcat cacgcgggcg
      841 ccgttgtact cctcgaccgc cttcccgacc tccatgtcga gcgcctcgat ctgcgcgagg
      901 acggcctcgg cctccgcctg cttctcggac gcgggatcgg ccccggcggg agtcgcaacg
      961 aggagggtcg ccagggctgc gacgaggatc gtcgccgctc ggcccgtgcg gcgcaggacg
     1021 ctcgctcgag ccgtggcccc gtggcgcggg cagggggatc tgctcgcagt cacaggggag
     1081 cgactctagc aacgcccgcc gacatgctgc aaacgccacc cgcgccgagg gcgccgcagt
     1141 ccgtcccggc cgccgcgaag ctggtagggt gcgcgaccgt gcgagcggct gcacgcaggc
     1201 atgcgcgtct cgcccgtctc gccgtcagcg tctttgcggc gacagccacc cttctggggg
     1261 cggcgggggc gcatgcagac caggcagggg aggctgcgcg ggacgcgcag ggcttcgccg
     1321 acgccgggga ccgcctcgcg gccgaggcgc aggcggtcac gctcgagctc tacgcgctcg
     1381 agtccgagct cgccgccgcc cgcgagcggc tcgcttccct ccaggcacgc gccgccgcgg
     1441 tcgaggagga gcgtgaggcc gcccgcacgc acctcgccgc agtgcgcgcg acgctcgcga
     1501 cggccgagga acggctcgcc gcccgcctgc gggagctcta cgtccagagc gatcccgacc
     1561 cgctggccgt cctcctgggc gcgcaatcgc tcgacgaggc cctcaccgcc ctcgactcgc
     1621 tcgggcgctt cgccgaccag gaccgcacgg tgctcgaaca ggtgcgcgcg gcccgcgcgg
     1681 ccgcgcacga cgcggtcgag aggctcgccg cccgcgaggc ggagctcgag gcgctcgtcg
     1741 ccgacgccgc ggacaccgag gcgtcgctcg ccgcatcgca ggagagccgc ggcgcgtacc
     1801 tcgcgggcct gcgggaggag cagcgcctga acgccgccca gctcgcccgg gcggaggagc
     1861 aggccgccgc ggcgcagcag aaggccgagg cgatccccgc cgcacagccc tcggtcgcgg
     1921 cgcccgcgcc ggtcggcggc ccggtcgcgg ggcccggccg gcagctgacc gtcgaggtga
     1981 ccgcgtacgc gctccccggc acgaccgcga cggggatccc ggtcggctgg ggcgtcgtcg
     2041 ccgtcgatcc gtccgtgatt ccgctcggca cccggatgac gatccccggc tacggcgagg
     2101 gcgtcgcggc cgacaccggc agcgcggtca agggccatgt gatcgacgtc tggatgccga
     2161 cccgcgagca ggcgctcgcc tggggccggc ggacgcttac gatcacgctc cactgagacc
     2221 ccgggcgacc gccctcgcgc gagccgccac tacaatccga ccgtccagcc acgaacccgg
     2281 ggtcgcccgt gccgaacgac gcgaggacgg agaacgtgaa cgagcagccg cgagtgctgc
     2341 tcgtcgacga ccatgacctc tttcggaccg ggctcgccaa gctgctgacc gagcagggcg
     2401 tccacgtgat cggcgaggct ccgacgggcg aagccgcgct caggctcgtc gacgagctcg
     2461 ccccggacgt cgtgatcatg gatctgaaca tgccgggcct cggcggcgtc gacgcgacgc
     2521 gcgagatctc ggcctcggcc ccgcgcacac gcgtcgtcgt cctgaccatc tccgaggacg
     2581 acgacgacat cgtcgacgcg atcatggccg gcgcgtgcgg ctacctcctc aagaactcga
     2641 ccgtgcagga gctcgtcgcc ggcatctccg ccgccgcgga cggcgactcg ctcatctcgc
     2701 cccaggtcgg gacgaaggtg ctccagcagc tgcgggccca ggcggcgggc gtccgccgcg
     2761 acacggccgc cccgcgcatc cagctctcgg agcgcgagct cgacgtcctc cgcctcatcg
     2821 cgatcggcaa ggacaacgcc cagatcgcgc aggagctctt catcagcccg aagacggtca
     2881 agaaccacat ctcgaacatc ctcatgaagc tccagatcga gaaccgcatc caggccgcgg
     2941 tctacgcggt ccggagcggg atcgtctaga gccgctcggc cgcgccgcgc aggacgctct
     3001 cgcgccgcgc gcggagcccg tcgagagacg cccgcagccg cgtcagctcg agctcgagct
     3061 cggcgaggcg ctccgacagg gcgacgaggt gcgccgcgcc ggcctcccct ccggcctcgg
     3121 cgaggccggc cgcgatctcg cgctccgtcc gcagccgctc gccctcgatc gcgagcgcct
     3181 cggcatagcc gtccgtgagc gtatgctcca ttgcctcgag gaggacatcc ggggacccct
     3241 cggaaggttg ctccacgagc gcctcgatcc ggtcgatgag agacggacga tcgcccaccg
     3301 acccattatc cgagccgccc gtggcacaag accatgggcc gaccggccta tctttcgccg
     3361 ccggagtcct accgtgagca ccgcccgcac ccgcctgctg ctcgcgctcg ccgtcgtgct
     3421 ctcggtcgcc gtctcggcgc cggccgccgc ggcccagtcg ctcgcgacgc ggctcgaccg
     3481 agcgctcaac acctccggcg tctcgtggag cgcgagcgga gcgatcgcgc tcaacctctc
     3541 gaacggccgc gtcgcgtacg cgcgaaacct cgacaagccg ttcgagccgg cgtcgaatca
     3601 gaagctcacc gtgacgctcg ccgcgctcca caagctcggg ccggactacc ggatcccgac
     3661 gcaggcgtac ggcgagggga ggcaggacgg cgacgtctgg cgcgggcgga tcgtcatcaa
     3721 gggcttcggc gaccccacgc tcacgacctc cgacctgaac acgctcgcgc gcaaggtgcg
     3781 cgacctcggg atcacgcgcg tgacgggcgg tgtgcgcggc gacgagacgt acttcgacac
     3841 gcggcgcacc gcgccgggct ggaagccgag cttctacaag gtctggtgcc cgccgctctc
     3901 cgcgctcgtc gtcgaccgcg cacgcgtgaa cgggacggtc gtcgaccgcc ccgcgctcgc
     3961 ggccgcgcac gccttccgcg ctgcgctcgt acggaacggc gtcgccgtga tgcgctcggc
     4021 gcgcgtcggg ccgctcggcg aggaggcggt cgcgctcgcg cgcgtccgct cgccgcggat
     4081 cgcgctcctc gtgcgccgga tgaaccgccc cagcgacaac ttctacgccg agatgctgct
     4141 gaagaagctc ggcgcgcact tccgcggcaa gggcacgagc ggagccggcg ccacggtcgt
     4201 acgccgggtg ttccgagcgc gcggcgtgcc gctcgcgggg atccggatcg ccgacggctc
     4261 gggcctctcc tcgctcgacc ggctgacgac gcgcgcgatc tcgcggatgc tcgtctcggc
     4321 gcggaaggac cccgtgatcg ggttgccgtt cttcaactcg ctcccgatcg cgggcgtgaa
     4381 cgggacgctc aaggaccgga tgcgccgcgg ccccgcccgg ggcgtcgtcc acgccaagac
     4441 gggcacgacg ctcgccgcct ccgcgctctc cgggtacgtc ggctcgcgct gggtgttctc
     4501 gatcctccag aacggctcgc ccgtcccctg gacgtcgtcg cgcgcgagcc aggaccgctt
     4561 cgcccagatc ctcgcaggcg gctagcaaag cgacgtatcc ttcgtcacat gcgggactcg
     4621 ctcatcgctg cgggggcggc cgcggcggtc gcgctcggcg ccggctgtgc cggcgaccgc
     4681 gactccgcgc cggcgacggc cccgcctgcg gcgcccgcgg ccgagcccgc cgtcgggaac
     4741 ggtgcgctcc tcttcac
//
LOCUS       DASTFC010000071         5297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_479376,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000071 DASTFC010000000
VERSION     DASTFC010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479376"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFO88_02090"
     CDS             <1..513
                     /locus_tag="VFO88_02090"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9544347.1"
                     /translation="IVHRLDRDTSGLLVVARSDAVHRALERSLRAREITREYLALVRG
                     RPRSRRGRIEAAIGRDRHDATRHSLDTATPREAVTEFEVVELLRAHALLRVRLETGRT
                     HQIRVHLAAIDLPVAGDPVYGVEGDLGLERQFLHAARLAFPHPVGGAPVEVESPLPDG
                     LAAALERARA"
     gene            complement(710..1498)
                     /locus_tag="VFO88_02095"
     CDS             complement(710..1498)
                     /locus_tag="VFO88_02095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9544348.1"
                     /translation="MAEKQAAAWEVAGEALAEASRCGVAPSLERLGRLGQLGSLAALV
                     EALGDEEALAGAAIAHAREREGVGLAAGEIVGELLALGRVLERRGMRREREAVDWCLL
                     LVFERVTAELGERARRDPLTGVLNHRAFHALVAAEVARARRYRGRLALLLFDLDRFKE
                     TNDAEGHAEGDRILRAVALALGDSARENDAVGRLGGDEFGVLLLQARPAAVAAFVERV
                     RSRFPADVSASAGSAFLAEVTGPAEELFALADRRLYDDKIARAA"
     gene            1670..2569
                     /gene="rpsB"
                     /locus_tag="VFO88_02100"
     CDS             1670..2569
                     /gene="rpsB"
                     /locus_tag="VFO88_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET9544349.1"
                     /translation="MSVVSMRELLEAGVHFGHQTRRWNPKMRRFLFTERGGIYIIDLQ
                     KTQQLLDEAHDFVRNIAERGGSVLFVGTKKQCQDAIESEAKRVGMPYVNHRWLGGLLT
                     NWRTISKRLERLEELRRLRDEGQLELLPPKERISMLAELEKLETNLGGVHEMKDTPDA
                     VLVIDLKKEQLAIREARRLNIPVVALVDTNCDPDDADFVIPGNDDAISSCSLVVRAIA
                     DGVEAGRKLVTEQEMARKQQAAAEAEAQAQARAAAEAAAAEQAATEGEVAPAEPAASA
                     ETDETPAPEEAPAAAETEEVKAE"
     gene            2566..3360
                     /gene="tsf"
                     /locus_tag="VFO88_02105"
     CDS             2566..3360
                     /gene="tsf"
                     /locus_tag="VFO88_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9544350.1"
                     /translation="MTEINAELVRELRARTGAGMMDCKRALVETGGDLEAAEKLLREK
                     GMADAAKRADRATTEGVVLTRIENGHGAIVAVACETEPVSKNEEFAAFAERVLDAVEA
                     EGPQAADRLDGERIELVAKLGENIEIRDAARLEATDGETLADYVHPPARKLGVLVRAQ
                     ASPELARMLAMHIAASPGTAYLTREQVPAETIEAEREIYEKLPEVEGKPDEIKTKIVD
                     GMLAKRLFADIVLLDQPWVHDSSLTVGKALAEHGAEVREFVRQSVA"
     gene            3389..4156
                     /gene="pyrH"
                     /locus_tag="VFO88_02110"
     CDS             3389..4156
                     /gene="pyrH"
                     /locus_tag="VFO88_02110"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9544351.1"
                     /translation="MSGALAEDAPAAEPAAFHRVLVKLSGESLMGEAAYGIDPERVAA
                     VAKQLGRLTELGIETAVVIGAGNIYRGMTAAADGMDRATADYAGMLATLLNCLAVQDA
                     LERAGVHTRILSALTVSEVAEPYIRRRAIRHLEKGRVVIFAAGTGNPFFTTDTAAALR
                     ALEIGAEGILMGKNGVDGVYDGDPNDDPSATFLPELTHREALERGLKVMDTTALSLCM
                     DNGLPIHVFGLDDERNIERVARGERVGTVISTPGRTR"
     gene            4153..4710
                     /gene="frr"
                     /locus_tag="VFO88_02115"
     CDS             4153..4710
                     /gene="frr"
                     /locus_tag="VFO88_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9544352.1"
                     /translation="MIDELLADAGRRMDKSVEATVHELNTVRTGRASAALLDRINVDY
                     YGTPTPLRQLATINVPEPRMLTIQPFDPSSVKAIERSIQESDLGLTPSNDGKIIRLPI
                     PQLTEERRKELVKVVRGLAEEGRVAVRNVRRDVMHHLKDLVRDGDVGDDEERRAEDKA
                     QKLTDEHVGRIDELLKRKEEEIMEV"
     gene            4738..>5297
                     /gene="uppS"
                     /locus_tag="VFO88_02120"
     CDS             4738..>5297
                     /gene="uppS"
                     /locus_tag="VFO88_02120"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET9544353.1"
                     /translation="MALLTKLRSLRGLKPAPEPELPDVARSVAIIMDGNGRWARRRRL
                     PVAAGHREGTRALRRTVEAAIDLGVESLCVYAFSTENWSRPEEEISDLMEIFVETIER
                     ELPDLARQGVRVRFIGRRDRATDALRAKMDELEAETAANARLALWIAFDYGGRAEIVG
                     AARRLVEEGVPAGEVDEEALRSRLYAP"
BASE COUNT          797 a         1937 c         1952 g          611 t
ORIGIN      
        1 atcgtccacc gcctcgaccg ggacacgtcg gggctgctcg tcgtcgcccg ctcggacgcc
       61 gtgcaccggg cgctcgagcg ctcgctgcgg gcccgcgaga tcacgcgcga gtacctcgcg
      121 ctcgttcgcg ggcggccgcg ctcgcgacgg gggcggatcg aggcggcgat cggccgcgac
      181 cggcacgacg cgacgcgcca ctcgctcgac accgcgacgc cgcgggaggc ggtcacggag
      241 ttcgaggtcg tcgagctcct ccgcgcgcac gcgctgctcc gggtgcgcct cgagacgggg
      301 aggacgcacc agatccgcgt ccatctcgcg gcgatcgacc tgcccgtcgc gggggacccc
      361 gtctacggcg tggagggcga cctgggcctc gagcggcagt tcctccacgc ggcgcgcctc
      421 gcgttcccgc atccggtcgg cggagcgccg gtggaggtcg agtcgccgct gccggacggc
      481 ctcgccgccg cgctcgagcg ggcgcgcgcg tagccgcccg cctgtggacg agtcgtcacg
      541 ctcccggcac ggtcccgtca cggacacgtg cgcccgcccg cgatagcgtt cgtcctggga
      601 cgctcgcgcc cccgatcggg gggaacggcc gcggtgcggc cgcggcggcg cgtggtggcg
      661 cggtcgcccg gccgccccac cgacgggtcg cgcgcgacgg ccgcgcgcgt caggccgcgc
      721 gcgcgatctt gtcgtcgtag agccggcggt cggcgagcgc gaagagctcc tccgccggcc
      781 cggtgacctc cgcgaggaag gccgagccgg cgctcgcgct cacgtccgcc gggaacctcg
      841 accgcacccg ctcgacgaac gcggcgacgg ccgccggcct cgcctggagg agcagcacgc
      901 cgaactcgtc tccgccgagg cgcccgaccg cgtcgttctc gcgggccgag tccccgagcg
      961 cgagggcgac ggcgcgcagg atccgatcgc cctccgcgtg gccctccgcg tcgttcgtct
     1021 ccttgaaccg gtcgaggtcg aagagcagga gcgccaggcg cccccggtac cgccgtgccc
     1081 gcgcgacttc ggccgccacg agcgcatgga acgcccggtg gttcaggacg ccggtgagcg
     1141 ggtcgcgcct cgcccgctcg cccagctccg cggtcacgcg ctcgaagacg aggagcaggc
     1201 accagtcgac ggcctcccgc tcgcgccgca tgccgcgccg ctcgagcacg cgcccgagcg
     1261 cgagcagctc gccgacgatc tcgcccgcgg ccaggccgac gccctcgcgc tcccgcgcgt
     1321 gcgcgatcgc cgcgcccgcg agcgcctcct cgtcgccgag cgcctcgacg agcgccgcga
     1381 ggctcccgag ctggccgagc cgcccgagcc gttcgagcga cggcgcgacg ccgcagcgcg
     1441 acgcctcggc cagggcctca cctgccacct cccatgcagc cgcctgcttc tccgccacgg
     1501 gcaaagggta gggaggcggt cggccggcgc ttgcgccgga cgctacactg tcggagctcc
     1561 gacccctacc cggcggcatc gaggcgggtg gcggtgccgg gctgcgtgtg cggcccggtt
     1621 ccatccgttc gccgccggcg caccgcaagc gaaaccgagg aggatcaccg tgtccgtcgt
     1681 ttccatgcgc gagctcctgg aggccggcgt ccatttcggc caccagacgc gtcgctggaa
     1741 ccccaagatg cgccgcttcc tgttcacgga gcgcggcggc atctacatca tcgacctgca
     1801 gaagacgcag cagctcctgg acgaggcgca cgacttcgtc cggaacatcg ccgagcgcgg
     1861 cggctccgtg ctcttcgtcg gcacgaagaa gcagtgccag gacgcgatcg agagcgaggc
     1921 gaagcgcgtc ggcatgccgt acgtgaacca ccgctggctc ggcgggctgc tgacgaactg
     1981 gcgcacgatc tcgaagcgcc tcgagcgcct cgaggagctc cgccgcctgc gcgacgaggg
     2041 gcagctcgag ctgctcccgc cgaaggagcg gatctccatg ctcgcggagc tcgagaagct
     2101 cgagacgaac ctcggcggcg tccacgagat gaaggacacg ccggacgccg tgctcgtgat
     2161 cgacctgaag aaggagcagc tcgcgatccg cgaggcgcgc cgcctgaaca tcccggtggt
     2221 cgccctcgtg gacacgaact gcgacccgga cgacgccgac ttcgtcatcc cggggaacga
     2281 cgacgcgatc agctcctgca gcctcgtcgt ccgcgcgatc gcggacggcg tcgaggcggg
     2341 ccgcaagctc gtcaccgagc aggagatggc ccgcaagcag caggcggccg ccgaggcgga
     2401 ggcccaggcc caggcgcgcg cggccgccga ggccgccgcg gccgagcagg ccgcgaccga
     2461 gggcgaggtc gccccggccg agccggcggc gagcgccgag acagacgaga cgcccgcgcc
     2521 cgaggaggcg ccggcggcag cagagacaga agaggtgaag gccgaatgac cgagatcaac
     2581 gccgagctcg taagagagct ccgcgcccgc acgggcgcgg ggatgatgga ctgcaagcgc
     2641 gccctcgtcg agacgggcgg cgacctggag gcggccgaga agctcctccg cgagaagggc
     2701 atggccgacg cggccaagcg cgccgaccgc gcgacgaccg agggcgtcgt cctcacgcgc
     2761 atcgagaacg gccacggcgc aatcgtcgcg gtcgcgtgcg agaccgagcc cgtctcgaag
     2821 aacgaggagt tcgccgcgtt cgccgagcgc gtcctcgacg ccgtcgaggc ggagggcccg
     2881 caggcggccg atcggctcga cggggagcgc atcgagctcg tcgcgaagct cggggagaac
     2941 atcgagatcc gtgacgcggc ccggctcgag gcgaccgacg gcgagaccct cgccgactac
     3001 gtccacccgc cggcccgcaa gctcggcgtc ctcgtccgcg cgcaggcgag cccggagctc
     3061 gcgcgcatgc tcgcgatgca catcgccgcg tcgccgggca cggcgtacct cacgcgcgag
     3121 caggtgccgg ccgagacgat cgaggccgag cgcgagatct acgagaagct gcccgaggtc
     3181 gagggcaagc cggacgagat caagacgaag atcgtggacg ggatgctcgc gaagcgcctg
     3241 ttcgcggaca tcgtgctcct cgaccagccg tgggtgcacg actcctcgct cacggtcggc
     3301 aaggcgctcg cggagcacgg cgccgaggtg cgcgagttcg tgcgccagtc cgtcgcctga
     3361 gagcgtcgga ggctctaagg tctcgcccat gagcggcgcg ctcgcagagg acgcaccggc
     3421 cgcggaaccg gccgccttcc accgcgtcct cgtcaagctg agcggcgagt cgctcatggg
     3481 cgaggccgcc tacggcatcg accccgagcg ggtcgcggcc gtggcgaagc agctcggccg
     3541 gctgaccgag ctcgggatcg agaccgcggt cgtgatcggc gccgggaaca tctaccgcgg
     3601 gatgacggcg gccgcggacg ggatggaccg cgcgaccgcc gactacgcgg ggatgctcgc
     3661 gacgctcctc aactgcctcg cggtgcagga cgcgctcgag cgcgcgggcg tccacaccag
     3721 gatcctgtcc gccctgacgg tgagcgaggt ggccgagccg tacatccgcc gccgcgcgat
     3781 ccggcacctc gagaagggcc gcgtcgtcat cttcgcggcc ggcacgggca acccgttctt
     3841 cacgacggac accgccgccg ccctgcgcgc gctcgagatc ggggcggagg ggatcctcat
     3901 ggggaagaac ggcgtggacg gcgtctacga cggcgacccg aacgacgacc cgtcggcgac
     3961 gttcctgccc gagctgaccc accgcgaggc gctcgagcgc ggcctcaagg tgatggacac
     4021 gaccgcgctc tcgctctgca tggacaacgg actgccgatc cacgtcttcg gcctcgacga
     4081 cgagcgcaac atcgagcgcg tcgcgcgggg cgagcgggtc ggcacggtga tctcgacccc
     4141 cgggaggacg cgatgatcga tgagcttctg gccgacgcag gccgccgcat ggacaagtcc
     4201 gtcgaggcga cggtgcacga gctgaacacc gtccggaccg gacgggcctc cgccgccctc
     4261 ctcgaccgga tcaacgtcga ctactacggc acgccgacgc cgctccgcca gctcgcgacg
     4321 atcaatgtcc ccgagccgcg gatgctcacg atccagccgt tcgacccgag ctcggtgaag
     4381 gcgatcgagc gctcgatcca ggagtcggac ctcggcctca cgccgtcgaa cgacgggaag
     4441 atcatccggc tcccgatccc gcagctcacc gaggagcgcc ggaaggagct cgtgaaggtc
     4501 gtccgcggcc tcgcggagga ggggcgcgtg gcggtgcgga acgtccggcg cgacgtcatg
     4561 caccacctga aggacctcgt ccgcgacgga gacgtcggcg acgacgagga gcgacgcgcg
     4621 gaggacaagg cgcagaagct caccgacgag cacgtcgggc ggatcgacga gctcctcaag
     4681 cgcaaagagg aagagatcat ggaggtctag ccctcgggcc gggcctctcc gacgacgttg
     4741 gcgctcctca cgaagcttcg ctcgctccgg gggctgaagc cggctcccga gccggagctt
     4801 ccggacgtcg cccgctcggt ggcgatcatc atggacggca acggccgctg ggcccggcgc
     4861 cgccgcctgc ccgtcgccgc gggacaccgc gagggcacgc gagcgctgcg gcggacggtc
     4921 gaggcggcga tcgacctcgg cgtcgagtcg ctctgcgtct acgcgttctc gaccgagaac
     4981 tggtcgcggc ccgaggagga gatctcggac ctcatggaga tcttcgtcga gacgatcgag
     5041 cgggagctcc cggacctggc ccggcagggc gtgcgcgtgc ggttcatcgg gcggcgcgac
     5101 cgggcgaccg acgcgctccg ggcgaagatg gacgagctcg aggcggagac ggccgcgaac
     5161 gcgaggctcg cgctctggat cgcgttcgac tacggcgggc gcgcggagat cgtcggggcg
     5221 gcgcggcggc tcgtggagga gggcgtgccg gcgggcgagg tggacgagga ggcgcttcgc
     5281 tcgcggctct acgcccc
//
LOCUS       DASTFC010000072         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1599886,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000072 DASTFC010000000
VERSION     DASTFC010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599886"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFO88_02125"
     CDS             complement(<1..227)
                     /locus_tag="VFO88_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544354.1"
                     /translation="MSTVTLHPPARRPLGATIWFVWMVAMWAVFFALLAADRLGDVWA
                     SVRDLPLVVEGILWLAFLPWLLGTAVWESSWP"
     gene            359..1543
                     /locus_tag="VFO88_02130"
     CDS             359..1543
                     /locus_tag="VFO88_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544355.1"
                     /translation="MIACLSIPVFELRAALRRRPELDGRPVALAPEPGTQPRVGPCTM
                     AAEAAGIQAGMRLSEALATCPELVLVEADEAGAEEEWERVLRRLEDAGFAVESPQAGC
                     ALFDTAGIERLAGGLQPALRRALDAVGREWHPRIGAGARRFTALAAASVAPPHRIVIV
                     DDDESALFLEPLPLDLLSLTPDQRDELSELGIRRLGELARLPGASVADRLGREAAEAW
                     RIVTGEEGPRVEGRRPASEIVEELEFPEAVGNELTLRHALAALLDRLLARPERGGRAP
                     RKVAVSARLVGGGSWRRALTLREATAEPDRLRLALGPKLAELTAPVLALRLELGELAE
                     SVGTQAELVRPRGSRLRDRLRDGLKQARLGAGVDAVCSVVEVAPWSRIPESRAILVPR
                     DD"
     gene            1564..1776
                     /locus_tag="VFO88_02135"
     CDS             1564..1776
                     /locus_tag="VFO88_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544356.1"
                     /translation="MVTRGVGASAEPVTVNRMQVARVREVWRVVDRWWTEAPVRRRYF
                     DLVLESGQNVVAYHDEEAGGWFTQRA"
     gene            1788..>2929
                     /locus_tag="VFO88_02140"
     CDS             1788..>2929
                     /locus_tag="VFO88_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9544357.1"
                     /translation="MAGYVELHCHSAYSFLDGASRPEELVLAAAEHGYPALALTDHDG
                     IYGSLEFAYAAKAFGVRPITGAEVTLADGSHVTLLVETPRGYANLCRLLTAAHAGRRL
                     DPGLDQALLEERNDGLVCLSGCARSGLAVRNPNAAARLARALGPERFLVELQRPLTRG
                     DARRNAALRELAGTLGARTVATGNAHAHAESRVRLQDALVAIRHRTSLEGCEPERRGN
                     DECALLAPDEVAERWRDDPASVAAAGELAERLTFDLTQELGYRYPDFSDGPDPADVQL
                     ARVTRAAFDERYRDAKPGLRRRAEERLREELALIAELGLSGFFLLHWEVLELARDVAA
                     EVRGAGSPRHLLPPGRGRGSSVGSIVCYLTGLSHVDPVAADLKLGR"
BASE COUNT          345 a         1113 c         1110 g          361 t
ORIGIN      
        1 ggccacgagc tctcccagac ggccgttccg agcagccagg ggaggaacgc gagccagagg
       61 atcccctcga cgacgagcgg caggtctcgc acggacgccc agacgtcgcc gagccggtct
      121 gcggcgagga gcgcgaagaa caccgcccac atcgcgacca tccagacgaa ccagatcgtc
      181 gcgccgagcg ggcgcctcgc gggcgggtgg agcgtgacgg tcgacatgcc ggcctccttt
      241 cgcgaccagt ctccgccgcg cacggccgcg gcgcatcggc gaatcgcccg accttcgctg
      301 cgaggattcg cggaggacct tgcaggggaa cacgtgttcg tgtagaacat acgttcccat
      361 gatcgcctgt ctctccatcc cggtgttcga gcttcgcgcg gcgctcaggc ggcggccgga
      421 gctcgacggg cgccctgtcg ccctcgcgcc cgagcccggc acccagccgc gcgtcgggcc
      481 ctgcacgatg gccgccgagg cggccggcat ccaggcgggg atgcgcctct ccgaggccct
      541 cgccacctgt cccgagctcg tcctcgtcga ggccgacgag gcgggggcgg aggaggagtg
      601 ggagcgcgtc ctccgccgcc ttgaggacgc gggctttgcg gtcgagtcgc cccaggccgg
      661 ctgcgcgctc ttcgacacgg cgggcatcga gcggctcgcg ggcgggctcc agccggcgct
      721 ccggcgggcg ctcgacgcgg tcggccgcga gtggcatccg cggatcgggg ccggcgcgcg
      781 ccgcttcacc gcgctcgccg cggcgagcgt cgcgccgccg caccggatcg tgatcgtgga
      841 cgacgacgag agcgcgctct tcctcgagcc gctcccgctc gacctcctct cgctcacccc
      901 tgatcagcgg gacgaactct cggagctcgg gatcaggcgc ctcggggagc tcgcgcggct
      961 cccgggcgcg tcggtcgccg accggctcgg gcgggaggcg gccgaggcgt ggcggatcgt
     1021 gacgggggag gaggggccgc gcgtcgaggg gcggaggccg gcgagcgaga tcgtggagga
     1081 gctcgagttc ccggaggcgg tcgggaacga gctgacgctc cgccacgcgc tcgccgcgct
     1141 cctcgaccgg ctcctggcgc ggcccgagcg gggaggccgg gcgccgcgca aggtggccgt
     1201 gtccgcgcgg ctcgtcgggg gcgggtcgtg gcggcgggcg ctgacgctcc gcgaggcgac
     1261 cgcggagccg gaccgcctcc ggctcgcgct cgggcccaag ctcgcggagc tcacggcgcc
     1321 cgtcctcgcc ctccggctcg agctcggaga gctcgcggag tcggtcggca cgcaggccga
     1381 gctcgtgcgc ccgcgcggga gccggctgcg cgaccggctc cgcgacgggc tcaagcaggc
     1441 tcggctcggc gccggggtcg acgccgtgtg cagcgtggtg gaggtggcgc cgtggtcgcg
     1501 gatccccgag agccgggcga tcctcgtgcc gcgcgacgac tgaacgagcc ccggcgggcg
     1561 ctcgtggtca cgcggggggt cggcgcgtcc gcggagccgg tgacggtgaa ccggatgcag
     1621 gtcgcgcgcg tccgcgaggt ctggcgcgtc gtcgaccgtt ggtggacgga ggcgcccgtc
     1681 cggcggcgct acttcgatct cgtcctcgag agcggccaga acgtcgtggc ctaccacgac
     1741 gaggaggcgg ggggatggtt cacgcagcgg gcctaggcag gtagtgcgtg gcgggctacg
     1801 tcgagctcca ctgccacagc gcgtactcct tcctcgacgg cgcctcgcgg cccgaggagc
     1861 tcgtcctcgc cgcggccgag cacggttacc cggcgctcgc gctcaccgac cacgacggga
     1921 tctacggctc gctcgagttc gcctacgcgg cgaaggcgtt cggcgtccgc ccgatcacgg
     1981 gcgccgaggt cacgctcgcg gacggctcgc acgtcacgct cctcgtcgag acgccgcgcg
     2041 gctacgcgaa cctctgccgg ctgctcacgg ccgcgcacgc gggccgccgc ctcgatccgg
     2101 ggctcgacca ggcgcttctc gaggagcgga acgacgggct cgtctgcctc tccggctgcg
     2161 cgcgctcggg cctcgccgtg cgcaacccga acgcggccgc ccgcctcgcc cgcgcgctcg
     2221 ggccggagcg gttcctcgtc gagctccagc ggccgctcac acgcggggac gcgcgacgga
     2281 acgccgccct gcgcgagctc gccgggacgc tcggcgcccg caccgtcgcg accggaaacg
     2341 cgcacgcgca cgccgagagc cgcgtccgcc tgcaggacgc gctcgtcgcg atccgccacc
     2401 gcacctcgct cgaaggctgc gagccggagc ggcgcgggaa cgacgagtgc gcgctcctcg
     2461 cgccggacga ggtggccgag cgctggcgcg acgatcccgc ctcggtcgcc gccgcgggcg
     2521 agctcgccga gcggctcacg ttcgacctca cgcaggagct cggctaccgc tacccggact
     2581 tctccgacgg gcccgacccg gcggacgtcc agctcgcgcg cgtgacccgc gccgcgttcg
     2641 acgagcgcta ccgcgacgcg aagcccggcc tgcgccgccg ggccgaggag cgcctgcgcg
     2701 aggagctcgc cctgatcgcc gagctcggcc tgtccgggtt cttcctcctc cactgggagg
     2761 tgctcgagct cgcgcgcgac gtcgccgccg aggtcagagg cgccggctcg ccgcgccacc
     2821 tgcttccgcc cggccgcggg cgcgggagct cggtcggctc gatcgtctgc tacctcaccg
     2881 ggctctcgca cgtcgacccg gtcgccgccg acctgaagct cggccgctt
//
LOCUS       DASTFC010000073         5873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_792483,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000073 DASTFC010000000
VERSION     DASTFC010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792483"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFO88_02145"
     CDS             complement(<1..1220)
                     /locus_tag="VFO88_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET9544358.1"
                     /translation="MGVFGSLYRRTATRLDRRYGWDRLPKWVAVYALAGLRSRLRKEN
                     LYDTETVHPLAPAGEPPPRALRARTLDGSFNDLDSPRMGAVGARFGRNVPLDRTFPEQ
                     PPALLEPSPRVVSRELLTRREFIPATTVNLLAGAWLQFEVHDWFSHGTNEPEEPWEVP
                     LGDGDDWPEDPMRIQRTRVDPTSDDDPTTPPTFVTADSHWWDASQIYGSEQGFADAIR
                     SGEHGRLRLDEQGLLPADLEQHVDLEGVAANFWLGLGLLHTLFTREHNAICDALRAEN
                     PGWDDERLYDTARLVNAALLAKIHTVEWTPAIIAHPTTTFAMNANWWGIVGERITKRV
                     GRIGGSEVLSGIPGSPTNHHAAPYSLTEEFVAVYRMHPLLPDDFTFRSLEDDRVLEER
                     TFPELGALQTRTRM"
     gene            1412..2347
                     /gene="cofD"
                     /locus_tag="VFO88_02150"
     CDS             1412..2347
                     /gene="cofD"
                     /locus_tag="VFO88_02150"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET9544359.1"
                     /translation="MARLTVLCGGLGGSRFADALVRAAGAGSVTVVGNVGDDLEVHGL
                     HVSPDLDTVLYTLAGLLDEERGWGVRDETYNARETAGRLGAETWFTLGDRDLGLHLVR
                     TELLRAGVPLSAVFARLAERLGVGIRLLPASDEPVRTMLATPDGELDFQTWFVRRRHA
                     DDVLAVRYAGAAEARPAPGVLEAIAEADVCFLAPSNPFVSIGPILAVPGVADAVAGRA
                     GPVVAVSPLVAGKALRGPLARMLETLGHEPSALGVARLYEGLASAFVLDPADAALAPA
                     VEALGLEAVVTPVVIVDPAVRGEVGRAILDATVPA"
     gene            2344..2934
                     /locus_tag="VFO88_02155"
     CDS             2344..2934
                     /locus_tag="VFO88_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9544360.1"
                     /translation="MIGIVGGSGDFGRGLGARLRAAGEEVALGSRTPRDDLVANEDAC
                     RDASVVFLSVPPEGVDSMSRGLAPLLGGKIVVSVASPVVFRDGKPGAAPGDVSLAELT
                     AAAVPDARVVAGFHTVSAKALAGDGALEEDVLLAGDDADAKAEVARLAGLVVSGRAVD
                     AGRLEVARWIEPLTAVLINVNRNYKAQSGILVTGLP"
     gene            2931..3629
                     /gene="cofE"
                     /locus_tag="VFO88_02160"
     CDS             2931..3629
                     /gene="cofE"
                     /locus_tag="VFO88_02160"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET9544361.1"
                     /translation="MTAGPVEILSLGGIPEIRPGDDLARLVGDALERAGGLAEGDVVV
                     VAQKAVSKAEGRLAQTDDTVRLILGEARAVRRRRGDLVIAETEHGFVCASAGVDRSNA
                     PGDGWAVLLPRDPDASARGLRSALAARFGPAPAVIVSDSFGRAWRQGTTDVAIGAAGL
                     APLLDLRGTTDARGRVLGSTQIAVADELAGAAELVLGKAAGVPAARIRGYRVPPGDGS
                     ARDLVMPPERDLFP"
     gene            3641..4240
                     /locus_tag="VFO88_02165"
     CDS             3641..4240
                     /locus_tag="VFO88_02165"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET9544362.1"
                     /translation="MLEAFTDFVSGSPWTYAYLFAVSAIDAFFPIVPSETSVIAAGVL
                     AASDDLVLPLVILVAAGGAILGDNISYFIGRTVGHRIAERFFSGERRKRLDQAERQLA
                     QRGGYLILVARFIPGGRTAVTLAAGTLEFPWRRFIAWDVAAGIIWASYASLLGYFGGR
                     TFEESPLKGLALAFGVAVAVTAIVEVVRWVRRRRTPTAA"
     gene            complement(4245..4409)
                     /locus_tag="VFO88_02170"
     CDS             complement(4245..4409)
                     /locus_tag="VFO88_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544363.1"
                     /translation="MRPDVWIPLLVLLSGATVARTLLVSARKLPATCARCGLRLERRA
                     LGEPVCRCGH"
     gene            4494..4742
                     /locus_tag="VFO88_02175"
     CDS             4494..4742
                     /locus_tag="VFO88_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544364.1"
                     /translation="MGILAVVFLTCAVLLVVGAEWPRLAGRLGSDGRAARSRRRRKAE
                     LTLIPGEEADQEDFAASVERDLASLPVIDAPDDRSSRR"
     gene            4777..4860
                     /locus_tag="VFO88_02180"
     tRNA            4777..4860
                     /locus_tag="VFO88_02180"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4811..4813,aa:Leu,seq:cag)
     gene            4971..5570
                     /locus_tag="VFO88_02185"
     CDS             4971..5570
                     /locus_tag="VFO88_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544365.1"
                     /translation="MTRAPAWITLHRAVAPVIVIALLALAVGVQAAGATVVEKGPYAD
                     QYTFVDDSCGFPLEVVGEVKGTYRLRQGKGKTASAFFLRDRFTYREVVTNLETGGWFV
                     LRGSGVFNEVKARRVSGTVFEFTAVEAGQPFVIEDSTGTVVVRDRGVLRHRILFDTLG
                     DDVPGGEFVLDLGTSVHGPHPGFADDFPFCEIAAELTGA"
     gene            5628..>5873
                     /locus_tag="VFO88_02190"
     CDS             5628..>5873
                     /locus_tag="VFO88_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544366.1"
                     /translation="MTMKLRIGLLAMVLAVGAVLASAGLAQPPVKEPLVSEPMEFPAG
                     VVCPFPVALESISGNESITFFASGRFHITGEGSTRVTN"
BASE COUNT          707 a         2083 c         2221 g          862 t
ORIGIN      
        1 tccatgcgcg tgcgcgtctg gagcgcgccg agctccggga acgtccgctc ctcgagcacc
       61 cggtcgtcct cgagcgagcg gaacgtgaag tcgtccggca agagggggtg catgcggtag
      121 acggcgacga actcctccgt cagcgagtac ggcgcggcgt ggtggttcgt cggcgagccg
      181 gggatcccgc tcagcacctc gctgccgccg atccggccga cgcgcttcgt gatccgctcg
      241 ccgacgatcc cccaccagtt cgcgttcatg gcgaacgtcg tcgtcgggtg ggcgatgatc
      301 gcgggcgtcc actcgaccgt gtggatcttc gcgagaagcg ccgcattcac gaggcgcgcg
      361 gtgtcgtaga ggcgctcgtc gtcccagccg gggttctccg cgcggagcgc gtcgcagatc
      421 gcgttgtgct cgcgcgtgaa gagcgtgtgc aggaggccga gcccgagcca gaagttcgcc
      481 gcgacgccct cgaggtcgac gtgctgctcg aggtcggcgg ggaggaggcc ctgctcgtcg
      541 aggcggagcc tcccgtgctc gccggagcgg atcgcgtccg cgaagccctg ctccgagccg
      601 tagatctggg acgcgtccca ccagtggctg tccgcagtca cgaacgtcgg cggtgtggtc
      661 gggtcgtcgt ccgaggtcgg gtcgacgcgg gtgcgctgga tgcgcatcgg gtcctcgggc
      721 cagtcgtcgc cgtcgccgag cggcacctcc cagggctcct ccggctcgtt cgtcccgtgg
      781 ctgaaccagt cgtgcacctc gaactggagc caggcgccgg cgaggaggtt caccgtcgtc
      841 gccgggatga actcgcggcg cgtgaggagc tcgcgcgaga cgacgcgcgg gctcggctcg
      901 aggagcgccg gcggctgctc cgggaaggtc cggtcgagcg ggacgttgcg gccgaagcgc
      961 gcgccgaccg cgcccatccg cggcgagtcg agatcgttga agctcccgtc gagcgtccgc
     1021 gcgcggaggg cgcgcggcgg cggctcgccg gccggcgcga gcgggtgcac tgtctccgtg
     1081 tcgtagaggt tctccttccg caggcggctg cgcagccctg cgagggcgta gaccgcgacc
     1141 cacttcggca ggcggtccca gccgtagcgg cggtcgaggc gggtcgcggt gcggcggtag
     1201 agagagccga agacccccac gggcctcgat cctcgcgcgc cgcgcccggt gtgtcaacgg
     1261 cgtggcgccg cgcgggccgt gacgctcccg gcacgcattc ggcacacggc gtgcgcctgt
     1321 cggcggtacg ctcgttctgg agggcctgag ggcccggtct cacaggcgcg tgtagatcgc
     1381 gtgcggcgcc ggggcggtag cgtctcgcgg catggctcgg ctcacggtgc tctgcggggg
     1441 gctcggcggc tcgcgcttcg cggacgcgct cgtgcgcgcg gcgggggcgg gctccgtgac
     1501 ggtggtcggg aacgtcggag acgacctcga ggtgcacggc ctgcacgtct cgcccgacct
     1561 cgacacggtg ctctacaccc tcgccgggct gctggacgag gagcggggct ggggcgtccg
     1621 ggacgagacg tacaacgcgc gcgagaccgc cgggcggctc ggcgcggaga cgtggttcac
     1681 gctcggcgac cgcgatctcg ggctccatct cgtccgcacg gagctcctgc gcgccggcgt
     1741 gccgctctcg gccgtcttcg cccggctcgc ggagcggctc ggggtgggga tccgcctcct
     1801 gccagcgtcg gacgagccgg tgcggacgat gctcgcgacg cccgacgggg agctcgactt
     1861 ccagacctgg ttcgtccggc ggcggcatgc cgacgacgtg ctggcggtgc gctacgcggg
     1921 cgccgccgag gcgcggccgg cacccggcgt gctcgaggcg atcgccgagg cggacgtgtg
     1981 ctttctcgcc ccgtcgaacc cgttcgtctc gatcgggccg atcctggccg tccccggcgt
     2041 ggccgacgcg gtcgcggggc gggcggggcc cgtcgtcgcc gtgtcgccgc tcgtcgcggg
     2101 gaaggcgctg cgcggcccgc tggcccgcat gctcgagacg ctgggtcacg agccgagcgc
     2161 gctcggcgtc gcgcggctct acgaggggct cgcgagcgcg ttcgtcctcg acccggccga
     2221 cgcggcgctc gcgccggccg tcgaggcgct cgggctcgag gccgtcgtca cgccggtcgt
     2281 tatcgttgac cccgcggtgc gaggcgaggt gggacgggcg atcctggacg cgacggtgcc
     2341 ggcgtgatcg gcatcgtcgg cggcagcggc gacttcggcc gcggcctcgg tgcgcgcctg
     2401 cgcgcggcgg gagaggaggt cgcgctcggc tcgcgcacgc cgcgcgacga cctggtcgcg
     2461 aacgaggacg cctgccgcga cgcctccgtc gtcttcctct ccgtcccgcc cgagggcgtc
     2521 gattccatgt cgcgcgggct cgcgcccctg ctcggcggga agatcgtcgt gagcgtcgca
     2581 agcccggtcg tcttccggga cgggaagccg ggcgcggccc ccggcgacgt ctcgctcgcc
     2641 gagctgaccg cggcggccgt ccccgacgcg cgggtcgtcg ccggcttcca cacggtgagt
     2701 gcgaaggcgc tcgcgggcga cggcgcgctc gaggaggacg ttctcctcgc gggcgacgac
     2761 gcggacgcga aggccgaggt cgcccgcctc gccgggctcg tcgtcagcgg ccgcgcggtc
     2821 gacgcggggc gcctcgaggt cgcgcggtgg atcgagccgc tcaccgccgt cctcatcaac
     2881 gtgaaccgga actacaaggc gcagagcggg atcctcgtga ccgggctccc gtgaccgcgg
     2941 gcccggtcga gatcctctcg ctcggcggga tccccgagat ccggccaggc gacgacctcg
     3001 cgcgcctcgt cggcgacgcg ctcgagcggg cgggcggcct cgcggaaggc gacgtcgtcg
     3061 ttgtcgccca gaaggccgtc tcgaaagcag agggccggct cgcgcagacc gacgacaccg
     3121 tccggctgat cctcggcgag gcccgcgccg tgcggcggcg ccggggcgac ctcgtgatcg
     3181 cggagacgga acacgggttc gtctgcgcga gcgccggcgt cgaccggtcg aacgcgcccg
     3241 gggacggctg ggccgtcctc ctgccgcgcg acccggacgc gtcggcgcgc gggctccgct
     3301 cggcgctcgc cgcccgcttc ggcccggccc cggccgtgat cgtgagcgac tcgttcggcc
     3361 gcgcatggcg gcaggggacg acggacgtcg cgatcggcgc cgccgggctc gcgccgctcc
     3421 tcgacctgcg cgggacgacc gacgcgcgcg gccgcgtcct cggctcgacg cagatcgccg
     3481 tcgcggacga gctcgcgggc gcggcggagc tcgtcctcgg caaggcggcc ggcgtcccgg
     3541 cggcgcgcat ccggggctac cgagtcccgc cgggcgacgg gagcgcgcgc gacctcgtga
     3601 tgccgcccga gcgcgacctt ttcccgtagc gtcttcggcc ttgctcgaag cgttcaccga
     3661 cttcgtctcc gggtccccct ggacgtacgc gtacctcttc gccgtatcgg cgatcgacgc
     3721 gttcttcccg atcgtcccga gcgagacgtc cgtgatcgcg gccggcgtgc tcgcggcgag
     3781 cgacgacctc gtcctcccgc tcgtcatcct cgtcgccgcc ggcggcgcga tcctcggcga
     3841 caacatctcg tacttcatcg gacgcaccgt cgggcaccgg atcgccgagc gcttcttctc
     3901 gggcgagcgg cgcaagcggc tcgaccaggc ggagcggcag ctcgcccagc gcggcggcta
     3961 cctcatcctc gtcgcgcggt tcatccccgg cgggcgcacc gccgtcacgc tcgccgcggg
     4021 cacgctcgag ttcccgtggc ggcgcttcat cgcgtgggac gtcgcggccg ggatcatctg
     4081 ggcctcgtac gcgtcgctgc tcggctactt cggcgggcgg acgttcgagg agagcccgct
     4141 caaggggctc gcgctcgcct tcggggtcgc cgtcgccgtg accgcgatcg tcgaggtcgt
     4201 gcgctgggtg cgcaggcgcc gcaccccgac ggcggcctag ccgcctagtg cccgcagcgg
     4261 cagacgggct cgccgagcgc ccgccgttcc aggcggagcc cgcagcgggc gcaggtcgca
     4321 ggcagcttgc gcgcggagac gagcagcgtg cgggcgacgg tcgctccgga gagcaggacg
     4381 aggagcggga tccacacgtc cgggcgcacg ccgtcatcat cggcaggaga cgcccggggg
     4441 taaacccttt ccgccgctgc cggaagggaa ggagcttcac tacaattcac ggcgtgggga
     4501 tcctcgcggt cgtctttctg acctgcgccg tcctcctggt ggtcggggcc gaatggccgc
     4561 gcctcgccgg ccggctcggc agcgacgggc gcgccgcgcg ctcgcgccgg cgccggaagg
     4621 ccgagctgac gctcatcccc ggcgaggagg cggaccagga ggacttcgcc gcgagcgtcg
     4681 agcgtgatct cgcgagcctc ccggtgatcg acgccccgga cgaccgctcc tcgcgccggt
     4741 aggcgcgcgg agcctcgcta caatcgggcg cgccacgcgg atgtggcgga attggtagac
     4801 gcgcacggtt caggtccgtg tgggcttcgg cccgtggagg ttcaagtcct ctcatccgca
     4861 ctctcgccaa accgtgcgag ggcgttgaga aagatccact tgacacgaat tcgggtgggt
     4921 gcttgagtcc cgcgtccacg cttctgcggt cgagacgaag gaggtgcctc atgacgagag
     4981 cacccgcgtg gatcacgttg caccgggcgg tcgcgcccgt gatcgtaatc gccctcctcg
     5041 cgctcgcggt cggcgtccag gcggccggcg ccacggtcgt cgagaagggt ccgtatgccg
     5101 accagtacac gttcgtcgac gactcgtgcg gcttcccgct cgaggttgtc ggcgaggtga
     5161 agggcacgta ccggctccgc caggggaagg ggaagacggc ctcggcgttc ttcctccgcg
     5221 accggttcac gtaccgcgag gtggtgacga acctcgagac cggtggttgg ttcgtcctcc
     5281 gcggtagcgg cgtcttcaac gaggtgaagg cgagacgggt gagcggcacc gtcttcgagt
     5341 tcaccgcggt cgaggcgggc cagccgttcg tgatcgagga ctcgacgggg acggtcgtcg
     5401 tccgggacag gggcgtcctg cggcaccgga tcctcttcga cacgctcggt gacgacgtgc
     5461 cggggggtga gttcgttctc gatctcggaa cgtccgtgca cggtccgcat ccgggcttcg
     5521 cggacgactt cccgttctgc gagatcgcag cggagctgac cggcgcatag cggcgccggc
     5581 gaatgccgcc cctcgcgatc catccacact gaacattccg aagggagatg acgatgaagc
     5641 tgcggatcgg tctacttgca atggttctcg cggtcggggc ggtgctcgcc tcggcgggac
     5701 tcgcccagcc gcccgtcaag gagccgctcg tgagcgagcc catggagttc ccggccggcg
     5761 tcgtctgccc gttcccggtc gctctcgagt cgatctcggg caacgagtcg atcacgttct
     5821 tcgcgagcgg ccggttccac atcaccggtg aaggaagcac ccgcgtcacc aac
//
LOCUS       DASTFC010000074         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1154987,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000074 DASTFC010000000
VERSION     DASTFC010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154987"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFO88_02195"
     CDS             <1..310
                     /locus_tag="VFO88_02195"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544367.1"
                     /translation="VADQLDVATVFVREWRSLDAERRDEILAERRRYEERIRALFREG
                     REGSELRTDLDENAAALLFLSAANWAYTWLHPGEDTDAVADAFFGLLVDGMRGYDTPA
                     "
     gene            414..866
                     /locus_tag="VFO88_02200"
     CDS             414..866
                     /locus_tag="VFO88_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544368.1"
                     /translation="MTRMTKLVTTLCVSVVAAFGFTGTAAAQVPPGNQAGDSLVNVQV
                     GDITILAPISVAAALCDVNVNVLAQQLDAGDATCEATAESVATPGTGGNGGGNEAGDS
                     LVNVQIGDITALIPISVAAAICDVNVNVLARQLRLGEATCEATGTSNA"
     gene            946..1845
                     /locus_tag="VFO88_02205"
     CDS             946..1845
                     /locus_tag="VFO88_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9544369.1"
                     /translation="MARLIVNPNASRVTPELVAAVERELGGVETVLTRGPGHAAELAA
                     EAEPGEPLYVLSGDGGFNEVVNGLPDGVPVAFLPGGGTSVLPRALGLPRDAVAAAHAL
                     AQGATGRTISLGHVEASLEGEPVADRRFTFSAGIGLDAELVRAVDRLGRRAGGRRPGD
                     VVFARELGRLLLAHRAGFGPALTVVGRGRAAFALVANCDPYSYAGRVPLHAAPEATFE
                     GGLDLAAPVELTAARIPAAALALVRPRPGRPRWLLRVHDADELRIECDRPLPLQVDGE
                     DLGDVTAATFRAERAALEALVAR"
     gene            1856..>2163
                     /locus_tag="VFO88_02210"
     CDS             1856..>2163
                     /locus_tag="VFO88_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9544370.1"
                     /translation="MAQIAAAEHELRRVIGDAEGVGDREVEGLSEGDLLALYRDMILL
                     RTYDERSVVYHRQGRIGTYAIYWNHEAMQAGAAYALADDDWIFPSYRESAIGLLRGLP
                     A"
BASE COUNT          270 a          790 c          801 g          302 t
ORIGIN      
        1 cgtggccgac cagctcgacg tggcgaccgt cttcgtgcgc gagtggcgct cgctcgacgc
       61 ggagcgccgc gacgagatcc tcgccgagcg ccgccgctac gaggagcgga tccgcgcgct
      121 cttccgcgag ggccgcgaag gcagcgagct gcgcaccgac ctggacgaga acgcggccgc
      181 gctcctcttc ctctcggcgg cgaactgggc ctacacgtgg ctgcatccgg gcgaggacac
      241 ggacgcggtc gcggacgcct tcttcggcct gctcgtcgac gggatgcgcg gctacgacac
      301 gcccgcctaa gagcgtcgcg acaacattca catcgaattt acacccgccc cgtttcccgg
      361 ggggcctggt ggggacgacg tgtcttcgtc aacgaaagta ggggggaacg gaaatgacca
      421 ggatgaccaa gctcgtaacg acgctgtgcg tcagcgtggt tgccgcgttc gggttcaccg
      481 gcacggcggc ggcgcaggtg ccgcccggca accaggctgg ggacagcctt gtgaacgtcc
      541 aggtcggcga catcaccatc ctcgcgccga tctcggtcgc ggctgcgctc tgcgatgtga
      601 acgtcaacgt gctcgcccag cagctcgacg ccggcgacgc cacgtgcgag gccacggccg
      661 agtcggtcgc caccccgggt acgggcggta acggcggcgg caacgaggcc ggcgactccc
      721 tcgtgaatgt ccagatcggc gacatcaccg cgctcattcc gatctcggtc gctgcggcga
      781 tctgcgacgt gaacgtgaac gttctcgccc gccagctccg gctcggtgag gcgacctgcg
      841 aggccacggg cacctcgaac gcctagcgcc ttctccgcgg ggcgggtggg cttcgggcct
      901 gcccgccccg cttcgtctcg gcgcgcgcat gcgatcctct tgcgcgtggc gcgcctgatc
      961 gtcaacccca acgcgagccg cgtgacgccc gagctggtcg cggcggtcga gcgcgagctc
     1021 ggcggcgtgg agaccgtcct cacgcgcggg ccgggccacg cggccgagct cgcggccgag
     1081 gccgagccgg gcgagccgct ctacgtcctg tccggcgacg gcggcttcaa cgaggtcgtg
     1141 aacggcctgc cggacggcgt gccggtcgcg tttcttcccg gcggaggcac gagcgtcctt
     1201 ccgcgggccc tcgggctgcc gcgcgacgcg gtcgccgccg cgcacgcgct cgcgcagggc
     1261 gcgacgggtc gcacgatctc gctcgggcac gtcgaggcgt cgctcgaggg cgagccggtc
     1321 gccgaccgcc gcttcacgtt ctcggcgggg atcggcctcg acgcggagct cgtgcgcgcg
     1381 gtcgaccgcc tgggacggcg cgcgggcggg cggcggcccg gcgacgtcgt cttcgcgcgc
     1441 gagctcgggc ggctgctcct cgcgcaccgg gcgggcttcg ggcctgcgct cacggtcgtc
     1501 ggtcgggggc gtgcggcgtt cgcgctcgtc gcgaactgcg acccgtactc gtacgccggg
     1561 cgcgtcccgc tccacgcggc gccggaggcg acgttcgagg gcgggctcga cctcgccgcc
     1621 cccgtcgagc tgacggccgc tcggatccct gcggcggccc tcgcgctcgt ccgcccgcgg
     1681 cccggccgtc cgcgctggct cctgcgcgtc cacgacgccg acgagctgcg gatcgagtgt
     1741 gaccgcccgc tgccgctcca ggtcgacggg gaggacctcg gcgacgtgac cgcggcgacg
     1801 ttccgggccg agcgcgccgc actggaggcg ctcgtcgcgc gataatgggc tgcacatggc
     1861 gcagatcgcg gcggcagagc acgagcttcg gagggtgatc ggcgacgcgg agggcgtcgg
     1921 cgaccgcgag gtcgaggggc tctccgaggg cgacctgctc gccctctacc gcgacatgat
     1981 cctgctccgg acgtacgacg agcgctcggt cgtctaccac cgccagggtc gcatcgggac
     2041 gtacgcgatc tactggaacc acgaggcgat gcaggccggc gcggcgtacg cgctcgcgga
     2101 cgacgactgg atcttcccct cctaccggga gtcggcgatc gggctcctcc gcgggctgcc
     2161 ggc
//
LOCUS       DASTFC010000075         6900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1369276,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000075 DASTFC010000000
VERSION     DASTFC010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369276"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /gene="clpX"
                     /locus_tag="VFO88_02215"
     CDS             complement(<1..1065)
                     /gene="clpX"
                     /locus_tag="VFO88_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET9544371.1"
                     /translation="MARATDGNEQLLCSFCGKSQRQVKKLIAGPGVYICDECIDLCNE
                     IIDEELTAPAALDLDNLPRPKEIYEVLNDYVVGQEEAKRTLSVAVYNHYKRVQMATAE
                     GDVELQKSNILLLGPTGCGKTLLAQTLARILNVPFAIADATALTEAGYVGEDVENILL
                     KLIQAADFDVKKAETGIIYIDEVDKIARKADNPSITRDVSGEGVQQALLKILEGTVAS
                     VPPQGGRKHPHQEFLSIDTTNILFICGGAFAGLEKIIGKRIGKNSVGFGADIKSRRDV
                     EASELLSEVMPEDLLSYGLIPEFIGRLPVVSAVHQLTREDLVQILLEPKNALVKQYQR
                     FFNYDSIELIFTEDALWEISD"
     gene            complement(1106..1714)
                     /gene="clpP"
                     /locus_tag="VFO88_02220"
     CDS             complement(1106..1714)
                     /gene="clpP"
                     /locus_tag="VFO88_02220"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET9544372.1"
                     /translation="MSPLVPMVVEQTSRGERAFDIYSRLLNERIIFLGTPIDDQIANL
                     IIAQLIHLESEDPDKDISLYINSPGGSVYAGLAVYDTMQYVKPDVQTVCVGIAMSMGA
                     VLLAGGTQGKRMALPNSKILIHQVSSGFQGQATDIEIQAREVINIKRRLEEILADHTG
                     QEHEKVSHDMERDYFMTADEAKEYGIIDTVQTQRGAGVAAAA"
     gene            complement(1711..3066)
                     /gene="tig"
                     /locus_tag="VFO88_02225"
     CDS             complement(1711..3066)
                     /gene="tig"
                     /locus_tag="VFO88_02225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET9544373.1"
                     /translation="MKTNVEELSGNRVRLDVEVPEADVKHAIEHAASDLAASARIPGF
                     RKGKAPLPLIMARLGRDAVWAEAVRTHLDGWFWTAAATSGVQPVSSPEVELGEDPSDD
                     GSFRFSATVAVLAKPEVPDWTALEVPAAQPEVPAELVDNELDVLRASVAELVPADRPA
                     QEGDTVVVDIEGELAGTQRDYVTEVGSGRLVDGLDEALVGMRAGETKTVSVAVGEGET
                     TEVELALKDVKEPVLPELDDELARSTSEFDTLAELRADVEGRLREHVEAEVEAAFRES
                     AVDALVEATEFDLHPELVDRRAAELWSGMTRSLAQRGLGPEQYLALTGQSQDDLLERL
                     RAEAGHAIKRELALDAVAEKLGLEVTDEEVETFVREQADGAGDDAEETLATLHERGTF
                     ESLRGDLRLRKALDEVAAGVQRIPVELAAARDKLWTPEKEKASSGMNIWTPGSEEAPN
                     R"
     gene            3206..4939
                     /locus_tag="VFO88_02230"
     CDS             3206..4939
                     /locus_tag="VFO88_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9544374.1"
                     /translation="MESVANVLDYVNLGLLTLVALTAVWLWRRGRGRAALWAAITFGT
                     LAFVVDFSRFLPEDPETTLEQVGQRALLVALVLFPYLLYRFAVSFEPPARRVRVATAL
                     LTLAMVAWTIALPELPGEDEPEPAWFTAYVIGFVVHWTLLTAVVAGRLWRGRRGQPTV
                     VRRRMQLIGLAVAAITVALLLSAFSDEGNAGVQLAAGILVTVSALGFLVGLAPPAALR
                     LIWRRPETVETQAAISRLMTATSEADVAREVLPPMARMVGARAVALRDAGGRVIGTHG
                     ASAEMLADAHASATHDEHGRVVVLDVPSGSLVVWTNPYAPFFGREELALLRSLGALTG
                     LALDRSRLFAHERAAREALERADELKSNFIALAAHELRTPVTAIDGIIQTLHSRSADL
                     GPERRAALEETLAAQSTNMRGLVDQLLDLSRLDAEAVAISPMTVPVRERLESVVASAA
                     GVRADDVVLEVDEGLEITVDPAALDRIVSNLVTNALRYGQPPVVVRAERADRHFRLAV
                     EDSGPGVPDEFVPSLFERFTRSRTGRDRVTGTGLGLAIARSYAAAHGGDLIYEPADPT
                     GARFRLVIPLQ"
     gene            4971..5056
                     /locus_tag="VFO88_02235"
     tRNA            4971..5056
                     /locus_tag="VFO88_02235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5005..5007,aa:Leu,seq:tag)
     gene            5089..6057
                     /locus_tag="VFO88_02240"
     CDS             5089..6057
                     /locus_tag="VFO88_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9544375.1"
                     /translation="MPDERPPATAVRAPEGVPAPERHEPVLRDPGLTDFTLRDWRAIL
                     KRTGKGALADGVTDVAASLAYYAFLAVPAVLLVTLGLFTVLGGEDTVDSIMGRLDGVV
                     PPEAVTLLEESLTRTLENQGGGIVMVVLGTVLALWTSTGAMGGLIRGLNRVYGTEEGR
                     GFVKQRLTALQMLACVVVAFALVVVLLVLGPVISDWLGGVLGLEGVFGWIWWTVQWPV
                     LLVGLLVAIGGLLYLGPDVSHPRFRLVTPGSLLAVLIWLVASGLFALYVGLFGSYNKT
                     WGSLAAVIIMLTWLWLSALAVLLGAELNAEVKRTWRHRQGEPAHED"
     gene            6085..6156
                     /locus_tag="VFO88_02245"
     tRNA            6085..6156
                     /locus_tag="VFO88_02245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6118..6120,aa:Arg,seq:tcg)
     gene            complement(6157..6573)
                     /locus_tag="VFO88_02250"
     CDS             complement(6157..6573)
                     /locus_tag="VFO88_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544376.1"
                     /translation="MSRSAPGRRIARRAAVLATIGFGVLAIFQALLAAGAALGHAAWG
                     GESAELSTAQRIGSVVSVVLWTCAAYVVLGRAGFWARASSSRLFRWGTWFLAGLTAIS
                     ALVNFASQSRWENFLWGPLALILAVLCATVARSRDS"
     gene            complement(6681..>6900)
                     /locus_tag="VFO88_02255"
     CDS             complement(6681..>6900)
                     /locus_tag="VFO88_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544377.1"
                     /translation="GVVTFHKIDDMTTRVMLQMDWDPEGLKEKAGSMLGRDEAQIKAD
                     LERFKELIESRGVETGAWRGEVSRGTTT"
BASE COUNT          871 a         2511 c         2389 g         1129 t
ORIGIN      
        1 gtcggagatc tcccagaggg cgtcctcggt gaagatcagc tcgatcgagt cgtagttgaa
       61 gaagcgctgg tactgcttga cgagcgcgtt cttgggctcg aggaggatct gcacgaggtc
      121 ctcgcgcgtc agctggtgca ccgccgagac gaccgggagc cggccgatga actcggggat
      181 gaggccgtac gagaggaggt cctccggcat gacctcggag agcagctcgc tcgcctcgac
      241 gtcgcggcgc gacttgatgt ccgccccgaa gccgacggag ttcttgccga tccgcttccc
      301 gatgatcttc tcgagcccgg cgaaggcgcc gccgcagatg aagaggatgt tcgtcgtgtc
      361 gatcgagagg aactcctggt gcgggtgctt ccgtccgccc tgcggcggca cggaggcgac
      421 cgtcccctcg aggatcttga ggagcgcctg ctggacgccc tcaccggaca cgtcgcgcgt
      481 gatcgacggg ttgtccgcct tgcgcgcgat cttgtccacc tcgtcgatgt agatgatccc
      541 ggtctccgcc ttcttcacgt cgaagtcggc cgcctggatg agcttgagga ggatgttctc
      601 gacgtcctcg ccgacgtagc cggcctccgt gagcgcggtc gcgtcggcga tcgcgaacgg
      661 gacgttcagg atccgcgcga gcgtctgcgc gaggagcgtc ttcccgcagc cggtcgggcc
      721 gagcaggagg atgttcgact tctgcagctc gacgtcgccc tcggcggtcg ccatctgcac
      781 gcgcttgtag tggttgtaga ccgcgaccga gagggtgcgc ttcgcctcct cctggccgac
      841 gacgtagtcg ttcaggacct cgtagatctc cttcgggcgc gggaggttgt cgaggtcgag
      901 cgcggcggga gcggtgagct cctcgtcgat gatctcgttg cagaggtcga tgcactcgtc
      961 gcagatgtag acgccgggcc cggcgatgag cttcttcacc tggcgctggc tcttcccgca
     1021 gaaactgcag aggagctgct cgttcccgtc ggtcgcgcga gccatagacc ttgttgtagc
     1081 ggctttcggc gcggctgcct agccgctacg ccgccgcggc gacccccgcg ccacgttgcg
     1141 tctggacggt gtcgatgatc ccgtactcct tcgcctcgtc ggccgtcatg aagtagtcgc
     1201 gttccatgtc gtgcgaaacc ttctcgtgct cctggccggt gtggtcggcg aggatctcct
     1261 ccaggcggcg cttgatgttg atcacctcgc gcgcctggat ctcgatgtcc gtcgcctggc
     1321 cctggaagcc gctcgagacc tggtggatga ggatcttcga gttcggcagc gccatgcgct
     1381 ttccctgcgt gccgccggcc aggaggacgg cgcccatcga catcgcgatg ccgacgcaca
     1441 cggtctgcac gtccggcttc acgtactgca tcgtgtcgta caccgcgagg cccgcgtaca
     1501 cggagccgcc cggcgagttg atgtagagcg agatgtcctt gtcgggatcc tcggactcga
     1561 gatggatgag ctgcgcgatg atcaggttcg cgatctggtc gtcgatcggg gttccgagga
     1621 agatgatgcg ctcgttcagc aggcgcgagt agatgtcgaa cgcacgctcg ccgcgcgacg
     1681 tctgctccac gaccatgggg actaggggac tcatcggttc ggtgcctcct cgctgcctgg
     1741 ggtccagatg ttcatcccgg aggaggcctt ttccttctcc ggggtccaaa gcttgtctct
     1801 cgccgccgcg agctcgaccg ggatccgctg cacgccggcc gccacctcgt cgagcgcctt
     1861 gcgcaggcgc aggtcgccgc gcaggctctc gaaggtgccg cgctcgtgga gggtcgccag
     1921 cgtctcctcc gcgtcgtcgc ccgcgccgtc cgcctgctcg cggacgaacg tctcgacctc
     1981 ctcgtccgtc acctcgaggc cgagcttctc cgcgaccgcg tcgagcgcga gctcgcgctt
     2041 gatcgcgtgg cccgcctcgg cgcggagccg ctcgaggagg tcgtcctgcg actggccggt
     2101 gagcgccagg tactgctccg gcccgagccc gcgctgggcg agcgagcggg tcatgccgga
     2161 ccagagctcg gcggcgcgcc ggtcgacgag ctccgggtgg aggtcgaact cggtcgcctc
     2221 gacgagcgcg tcgaccgcgg actcgcggaa cgcggcctcg acctcggcct cgacgtgctc
     2281 gcgcaggcgg ccctcgacgt ccgcgcgtag ctcggcgagc gtgtcgaact cgctcgtcga
     2341 gcgtgcgagc tcgtcgtcga gctccggcag caccggctcc ttcacgtcct tgagcgcgag
     2401 ctcgacctcg gtcgtctcgc cctcccccac ggcgacggac acggtcttcg tctcgccggc
     2461 gcgcatgccg acgagcgcct cgtccagccc gtccacgagc cggccgctgc ccacctcggt
     2521 cacgtagtcg cgctgcgtgc ccgcgagctc gccctcgatg tccacgacga cggtgtcgcc
     2581 ctcctgcgcc ggccggtcgg ccgggacgag ctccgcgacg ctcgcgcgga gaacgtcgag
     2641 ctcgttgtcc acgagctccg ccgggacctc gggctgcgcg gcgggcacct cgagcgccgt
     2701 ccagtcgggc acctcgggct tcgcgagcac ggcgacggtc gcgctgaacc ggaacgagcc
     2761 gtcgtcggac gggtcctcgc cgagctcgac ctccggggag ctgaccggct gcacgccgga
     2821 cgtcgccgcg gccgtccaga accagccgtc gagatgcgtc ctcacggcct ccgcccagac
     2881 ggcgtcgcgg ccgagccgcg ccatgatgag cggcagcggc gccttcccct tccggaagcc
     2941 ggggatccgc gcgctcgcgg cgaggtcgga cgccgcgtgc tcgatggcgt gcttcacgtc
     3001 cgcctcgggc acctcgacgt cgagccggac gcggttcccg gagagctctt cgacgttcgt
     3061 cttcaccgtc cggccattct gccagcggtc ggccgccccg cgggccgggc ccgtcgcgcc
     3121 cggcaggact cgtgttcgcc gcctggaaat tgcgggcagc cgctgcgggg cggccctaga
     3181 gtacgtgccg agggtcgtcg ttctcatgga atcggtcgca aacgttctcg actacgtcaa
     3241 cctggggctg ctcacgctcg tagccctgac ggcagtctgg ctctggcggc gcggtcgcgg
     3301 acgggcggcg ctctgggcgg cgatcacctt cgggacgctc gcgttcgtcg tcgacttctc
     3361 gcgcttcctg ccggaggatc cggagacgac gctcgagcag gtcggccagc gcgcgctcct
     3421 cgtcgcgctc gtcctcttcc cctacctcct ctaccgcttc gccgtctcgt tcgagccgcc
     3481 ggcgcgccgc gtccgcgtcg cgaccgccct gttgacgctc gccatggtcg cgtggacgat
     3541 cgcgctcccc gagctcccgg gcgaggacga gccggagccc gcctggttca cggcgtacgt
     3601 gatcgggttc gtcgtccact ggacgctcct gacggccgtc gtcgccggcc gtctctggcg
     3661 ggggcgtcgc ggccagccga cggtcgtccg ccgccggatg cagctgatcg ggctcgcggt
     3721 cgccgcgatc accgtggcac tcctcctgtc ggcgttcagc gacgagggca acgcgggggt
     3781 ccagctcgcg gcggggatcc tcgtgacggt gagcgcgctc gggttcctcg tcgggcttgc
     3841 gccgccggcc gcgctccggc tgatctggcg acgcccggag acggtcgaga cgcaggccgc
     3901 gatctcgcgc ctcatgacgg cgacgagcga ggccgacgtc gcccgggagg tcctcccgcc
     3961 gatggcccgc atggtcggcg cccgggccgt tgccctgcgc gacgcgggcg gccgcgtgat
     4021 cgggacgcac ggcgcctccg ccgagatgct cgccgacgcc cacgcgtcgg ccacccacga
     4081 cgagcacggg cgcgtcgtcg tgctcgacgt tccgtcgggc tcgctcgtcg tctggacgaa
     4141 cccgtacgcg ccgttcttcg gacgcgagga gctcgccctc ctgcgctcgc tcggcgccct
     4201 caccgggctc gcgctcgacc gctcgcggct cttcgcgcac gagcgcgcgg cgcgcgaggc
     4261 gctcgagcgc gcggacgagc tcaagtcgaa cttcatcgcg ctcgccgccc acgagctccg
     4321 gacgccggtc accgcgatcg acggaatcat ccagacgctg cacagccgct cggcggacct
     4381 cggcccggaa cgccgggcgg cgctcgaaga gacgctcgcc gcccagagca cgaacatgcg
     4441 cgggctcgtc gaccagctcc tcgacctctc ccggctcgac gcggaggccg tggcgatcag
     4501 ccccatgacg gtgcccgtgc gcgagcggct cgagtcggtc gtcgcctcgg cggcgggcgt
     4561 ccgggccgac gacgtcgtcc tcgaggtgga cgaggggctc gagatcaccg tcgatccggc
     4621 cgcgctcgac cggatcgtgt cgaacctcgt cacgaacgcg cttcgctacg gacagccgcc
     4681 cgtcgtcgtc cgggccgagc gggccgaccg gcacttccgc ctcgccgtcg aggactccgg
     4741 gccgggcgtc ccggacgagt tcgtcccctc gctcttcgag cgcttcacgc gcagccgcac
     4801 cggccgtgac cgcgtgaccg ggacggggct cgggctcgcg atcgcccgct cgtacgcggc
     4861 cgcgcacggc ggcgacctca tctacgagcc ggccgaccca accggcgcgc gcttccggct
     4921 cgtgatcccg ctccagtagg gagccgtccg gtgcggcaca atccgcgcat gcgggcgtgg
     4981 cggaactggc agacgcgacg ggtttaggtc ccgttgggcc ttcgggccct tggaggttcg
     5041 agtcctctcg cccgcatcga atcccgcggc gcgtggaaga cccttcgcat gccggacgag
     5101 cgccctcccg cgaccgccgt gcgcgccccc gagggcgtgc ccgcgcccga acggcacgag
     5161 cccgtgctcc gcgaccccgg cctgaccgac ttcacgctcc gcgactggcg cgcgattctc
     5221 aagcgcaccg gcaagggcgc actggccgac ggggtgaccg acgtcgccgc ctccctcgcg
     5281 tactacgcct ttctcgccgt cccggccgtc ctgctcgtga cgctcgggct cttcaccgtc
     5341 ctcgggggcg aggacacggt cgacagcatc atgggccggc tcgacggcgt cgtcccgccc
     5401 gaggcggtca cgctcctcga ggagagcctg acccgcacgc tcgagaacca gggcggcggg
     5461 atcgtcatgg tcgtcctcgg caccgtgctc gcgctctgga cgtcgaccgg cgcgatgggc
     5521 ggcctcatcc gcgggctcaa ccgcgtctac ggaaccgagg aagggcgggg gttcgtgaag
     5581 cagcggctga cggcgctcca gatgctcgcg tgcgtcgtcg tcgccttcgc gctcgtcgtc
     5641 gtcctcctcg tgctcggccc ggtcatctcc gactggctcg gcggcgtgct cgggctcgag
     5701 ggcgtcttcg gttggatctg gtggacggtg cagtggccgg tgctcctggt cggcctgctc
     5761 gtcgcgatcg gcgggctcct ctacctcggg ccggacgtct cgcacccgcg cttccggctc
     5821 gtgacgccgg ggtcgctgct cgcagtcctg atctggctcg tcgcgtcggg gctcttcgcg
     5881 ctctacgtcg gcctcttcgg ctcctacaac aagacgtggg ggtcgctcgc ggccgtgatc
     5941 atcatgctca cctggctctg gctctccgcg ctcgccgtcc tcctcggcgc cgagctgaac
     6001 gccgaggtca agcgcacctg gcgccatcgc cagggcgagc cggcgcacga ggactgacgc
     6061 ggggcggggc tacactcggg ctgcggcccc gtagctcagt ggatagagcg gcagacttcg
     6121 aatctgcgtg cggaggttcg actcctcccg gggccactag gagtcgcggc tgcgcgcgac
     6181 cgtcgcgcag aggaccgcga ggatcaacgc tagcgggccc cagaggaaat tctcccagcg
     6241 gctctgcgag gcgaagttga cgagcgcgct gatcgcggtg aggccggcga ggaaccacgt
     6301 tccccaccgg aagaggcgcg acgagctcgc ccgcgcccag aagcccgcgc gaccgagcac
     6361 gacgtacgcg gcgcacgtcc acaggacgac ggagaccacg ctgccgatcc gctgggccgt
     6421 cgagagctcc gcggactccc ctccccaggc agcgtggccg agagcagcac ccgctgcgag
     6481 cagcgcctgg aagatcgcca gaacgccgaa gccgatcgtc gccagaaccg ccgctcgacg
     6541 cgcgatacga cgacctggcg cggaccggct catcctcggc cgtccacgat agtcgccggc
     6601 tcggctcgct tcggccgcga gaacgcggaa ggccgggctc tcgcccggcc ttctcgacgg
     6661 ttcttccgct gcggagacga ctaggtggtc gtgccgcggc tgacctcgcc gcgccaggcg
     6721 cccgtctcga cgccgcggct ctcgatgagc tccttgaagc gctcgagatc cgccttgatc
     6781 tgggcctcgt cgcggccgag catcgagccg gccttctcct tcaagccttc cggatcccag
     6841 tccatctgga gcatgacccg ggtggtcatg tcgtcgatct tgtggaacgt gacgacgccg
//
LOCUS       DASTFC010000076         7119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1171480,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000076 DASTFC010000000
VERSION     DASTFC010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171480"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFO88_02260"
     CDS             complement(<1..492)
                     /locus_tag="VFO88_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9544378.1"
                     /translation="MPRNPLDEAGRALRQLGERLRETGGPEGEWIAEQLDRLQPDARR
                     RAPTEPEKTLEELQAELDALVGLDTVKEQVRALVAFLQVQALRKESGLAEVATSQHLV
                     FLGNPGTGKTTVARLLAQMYRSMGLLRRGHLVEVDRAGLVGQWVGSTALKTDRVIRRA
                     LDGV"
     gene            552..1988
                     /locus_tag="VFO88_02265"
     CDS             552..1988
                     /locus_tag="VFO88_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET9544379.1"
                     /translation="MRARLLDEANRKWLTLAAVSFGLFMIMLDNTVVNVALPSIQREL
                     GVGLSELEWIVTGYALAFAALMLTGGKLADLFGRRLVFVVGLAIFTLASLACGLAGSG
                     DVLIGARIVQGVGAALMNPATLSIIAATFPARQRGTAIGIWAGVSALALAIGPLVGGL
                     ITQHVNWNWIFFINVPVGILAIAASFLVIRESRDESEVQRLDLPGLLTSGIGLFALTY
                     GLIEANTHGWTSTRILVSFTVAVVALVGFVLLERHQRVPMLDLTLFRSGTFAGANVVV
                     LLVALAMFGVFFFVSLYMQNILGYSPVQAGAAFLPMTILVILVSPVAGRMSDRIGSRW
                     LMTAGMTLLSIQLLYFSQLGASATYWNLLPAMLVGGLGMSLTMTPSAAAAMRALPVDK
                     SGVGSAVLNAFRQVGGSVGIALMGAIVAHEAAGRRTPAAFMDGFSLALLVAAGIAFVG
                     AIVAAALVRPHEMTGTGAPEAPAVPEAV"
     gene            1985..2584
                     /locus_tag="VFO88_02270"
     CDS             1985..2584
                     /locus_tag="VFO88_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9544380.1"
                     /translation="MSPGQRLPARERRAHVLECACRVFSQGSYRGTTTAELARSAGVT
                     EPILYRHFESKRHLYLACLDEIWQALKARWDEAIAAEPDPAAWLRVLGHTFFEAERDE
                     PVISALWVQALAEAGEDEEIQRYMRDHVHEVHAYVASVIERGQQEGGFLADRDPRAEA
                     WLFVSLGLLRMASLRLGGLVDDDFPAIVAARRRWLTGRD"
     gene            3030..4379
                     /locus_tag="VFO88_02275"
     CDS             3030..4379
                     /locus_tag="VFO88_02275"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9544381.1"
                     /translation="MTDSPLLLKTRAGSTARPSLGERVRQLRINAGLTQTDLAGERFS
                     KEYVSQIERGKTRPTDETIAWLAERLGVDAGFLLHGVSSGERERVESVIARAEATLES
                     HDYEGTLPILEGVAEALTKVSAPDLELRALTAEAWARMYIGDVKDAARLLERARVLTE
                     EPSFTDVDRADVLFRLGACRYNLSSIATAVSLLTQAIELADRSELPSDRLRALALEWR
                     SRCYQRQRDWEAAREDVERALELARGIDDRYALAQAHFQASLVAERQGRWVLARTHAE
                     QAKGLYEEIAHQANVGALLNNLGGLNFLLGKPEEAKAYLKDAFRVALEVGSDMEAGYA
                     VSSLAQVHLRTGELDLADEQARHALELLKGREDDLQDEIGNVQLVLGRSLLERGRLDE
                     AEEWFRRAEANFDQLSSASHRAAAWIAQGDLAAMRGDDRSAADLYRRAAEALQDFHF"
     gene            4774..5631
                     /locus_tag="VFO88_02280"
     CDS             4774..5631
                     /locus_tag="VFO88_02280"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET9544382.1"
                     /translation="MGKIDAYAKPVLVDTDWLAEHLGDDGVVVAEVDESPELYDEGHI
                     PGAVKLHWQEDLQDPVERDLVGREDFERLLGSLGVGSDTTVVLYGDKNNWFAAYAYWY
                     LKVYGHGDVRILDGGRQKWIDERRELTADAPSPRAVEYHAGERDESIRAYRDQVREWI
                     GAGDRALVDVRSPGEYAGDLVAPPGYEQEGAQRAGHIPTAASIPWASAVADDGTFRPA
                     EELRDLYAGKGVTADKEVTAYCRIGERSAHTWFVLHELLGFEKVRNYDGSWTEWGNLV
                     DVPIEKGAG"
     gene            complement(5645..6529)
                     /locus_tag="VFO88_02285"
     CDS             complement(5645..6529)
                     /locus_tag="VFO88_02285"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HET9544383.1"
                     /translation="MTTLEPADRLALGYGCQSMTAPLRRVLVRPPTADGCAAWQAFEW
                     RSEPDTAKIAEEHEAFRALLAEAGAEVIVAGTPLAAATDAIYTYDPVLVTDEGALLLR
                     PGKPGRRAEIDAMAADLVEAGVPIRGRLEAPATAEGGDTVWLDERTLLVGRGYRTNDA
                     GVEGLAELLPDVDVIAFDLPHLNGPGEVLHLMSLVSPLDTDLALVYLPYMPVRLVELL
                     QERGIAFVEVPDEEFWSMGPNVLALAPRVALALEGNPETRRRMEAAGVDVRVYSGEEL
                     SKKGDGGPTCLTRPILRA"
     gene            6583..>7119
                     /gene="selD"
                     /locus_tag="VFO88_02290"
     CDS             6583..>7119
                     /gene="selD"
                     /locus_tag="VFO88_02290"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET9544384.1"
                     /translation="MESATRLRGLEAGGGCAAKYPAARLRELLAGFVPAEADDLLVGL
                     DPADDAAVYRLDDERALVFTIDFFPPMLDDPALYGAIAAANALNDVFAMGGRPLVALS
                     VAAFPEELPIETVRAILDAASAKTEEAGALLAGGHTIRDDEPKYGLAVVGTVHPGALW
                     RKDGARPGDALFLTKPLGT"
BASE COUNT          935 a         2635 c         2506 g         1043 t
ORIGIN      
        1 cacgccgtcg agcgcccgcc ggatgacacg gtcggtcttg agcgccgtcg agccgaccca
       61 ctggcccacg agcccggcgc ggtcgacctc cacgagatgc ccgcgccgga ggaggcccat
      121 cgagcggtac atctgcgcga gtaggcgcgc gacggtggtc ttcccggtgc ccgggttccc
      181 gaggaagacg aggtgctggc tcgtcgccac ctccgccagg ccgctctcct tgcggagcgc
      241 ctgcacctgg aggaacgcga cgagggcgcg cacctgctcc ttcaccgtgt cgaggccgac
      301 gagggcgtcg agctccgcct ggagctcctc gagcgtcttc tccggctcgg ttggcgcgcg
      361 ccggcgggcg tcgggctgca gccggtcgag ctgctcggcg atccactcgc cctcgggccc
      421 gccggtctcg cgcaggcgct cgccgagctg gcggagcgcc cgtcctgcct cgtcgagcgg
      481 gttgcgcggc acggcgccac ggtacccacc tgacgcttct ccaccgcctt agtgcttgct
      541 aacatcgacc cgtgcgcgcg cgactgctcg acgaggcgaa ccggaagtgg ctgaccctcg
      601 cggccgtctc cttcgggctc ttcatgatca tgctcgacaa cacggtcgtg aacgtcgcgc
      661 tgccctcgat ccagcgggag ctcggcgtcg ggctctcgga gctcgagtgg atcgtgaccg
      721 gctacgccct cgcgttcgcg gcgctcatgc tcaccggcgg gaagctcgcc gacctcttcg
      781 gccgccggct cgtgttcgtc gtcggcctgg cgatcttcac gctcgcctcg ctcgcctgcg
      841 ggctcgccgg ctcgggcgac gtactcatcg gcgcgcggat cgtccagggc gtgggggcgg
      901 cgctcatgaa cccggcgacg ctctcgatca tcgccgcgac cttcccggcc cgccagcgtg
      961 gcaccgcgat cggcatctgg gcgggcgtgt ccgctctcgc gctcgcgatc ggcccgctcg
     1021 tcggcggcct catcacccag cacgtgaact ggaactggat cttcttcatc aacgtcccgg
     1081 tcggcatcct cgcgatcgcg gcgagcttcc tcgtcatccg cgagtcgcgc gacgagtcgg
     1141 aggtccagcg gctcgacctg cccgggctcc tgacctcggg gatcggcctc ttcgcgctca
     1201 cgtacggcct catcgaggcc aacacgcacg gctggacgtc gacccgcatc ctcgtctcct
     1261 tcacggtggc ggtcgtcgcg ctcgtcgggt tcgtcctgct cgagcgccac cagcgcgtgc
     1321 cgatgctcga cctgacgctc ttccgcagcg ggaccttcgc gggcgcgaac gtcgtcgtcc
     1381 tgctcgtcgc gctcgcgatg ttcggcgtct tcttcttcgt ctcgctctac atgcagaaca
     1441 tcctcggcta ttcgcccgtg caggccggtg cggcgttcct gccgatgacg atcctcgtga
     1501 tcctcgtctc tcccgtcgcc gggcgcatgt ccgaccggat cggctcgcgc tggctcatga
     1561 ccgccgggat gacgctgctc tcgatccagc tcctctactt ctcgcagctc ggcgcgagcg
     1621 cgacgtactg gaacctcctg cccgcgatgc tcgtcggcgg gctcggcatg tcgctcacga
     1681 tgaccccgag cgcggccgcc gcgatgcgcg ccctccccgt ggacaagagc ggcgtcggct
     1741 cggccgttct gaacgccttc cgccaggtcg gcgggtcggt cgggatcgcg ctcatgggcg
     1801 cgatcgtggc ccacgaggcc gccggccggc gcacgccggc cgccttcatg gacggcttct
     1861 cgctcgcgct tctcgtcgcc gccgggatcg ccttcgtcgg cgcgatcgtg gccgcggcgc
     1921 tcgtgcgccc gcacgagatg accggtacgg gcgcgcccga ggcgcccgcg gttccggagg
     1981 ccgtatgagc ccgggacagc gcctgcctgc ccgcgagcgc cgggcgcacg tcctcgagtg
     2041 cgcctgcagg gtcttctcgc agggcagcta ccgcgggacg acgaccgccg agctcgcccg
     2101 ctccgccggc gtgacggagc cgatcctcta ccgccacttc gagtcgaagc gccacctcta
     2161 cctggcctgc ctggacgaga tctggcaggc gctcaaggcg cgctgggacg aggcgatcgc
     2221 cgccgagccg gatcccgcgg cgtggctccg cgtcctcggc catacgttct tcgaggccga
     2281 gcgcgacgag ccggtcatct ccgcgctctg ggtgcaggcg ctcgccgagg cgggcgagga
     2341 cgaggagatc cagcgctaca tgcgcgacca cgtccacgag gtgcacgcct acgtcgcctc
     2401 cgtcatcgag cggggccagc aggagggcgg cttcctcgcc gaccgcgacc cgcgcgccga
     2461 ggcgtggctc ttcgtctccc tcggcctgct ccggatggcg agcctgcgcc tcggcggcct
     2521 cgtcgacgac gacttcccgg cgatcgtcgc ggcacgccgc cgctggctaa ctggtcgcga
     2581 ctaggacgcg cggcgtatca acgctgagcc gccggcgcgt cccggacgcc ccgtggccgc
     2641 gtcctcagtc tcgcgcgtag cgtcgctacg cgcgctcctt gcgtccttgc cacgcaacgt
     2701 ccgcgacacg ccgacgacga acggaacacg ccgcgcgccc cagccgcgac cgtgcgatta
     2761 acccaacgag ggattcacgc ggcgagccga acgggcgacg gtggaggtat gccgcatcct
     2821 ctcgaggtct ttaaaggcga cggcccgaag gcttcgggtc tgcggcccac ccagcctccg
     2881 ggcgtctcgt cgcctatctc tctggcgcgc cgcccgcacc tgcctggcgt gtggcgagga
     2941 gcgagcaact cggactatag gcacacggcc gaaaaagtca agtggggttg tgaagcagat
     3001 ttccgcccct atagttcact taggctcaaa tgaccgactc gccgctcctg ctcaagaccc
     3061 gcgccggctc gaccgcacgg cccagcctcg gcgagcgcgt gcgtcagctc cgcatcaacg
     3121 cggggctcac gcagaccgat ctggccggcg agcggttctc gaaggaatac gtgagccaga
     3181 tcgagcgcgg caagacgcgc ccgaccgacg agacgatcgc ctggctcgcg gagcggctcg
     3241 gcgtcgacgc cggcttcctg ctccacgggg tctcctccgg cgagcgggag cgcgtcgaga
     3301 gcgtgatcgc ccgcgccgag gcgacgctcg agtcgcacga ctacgagggc acgcttccga
     3361 tcctcgaggg ggtcgccgag gcgctcacga aggtctcggc gcccgatctc gagcttcgcg
     3421 ccctcacggc cgaggcctgg gcgcgcatgt acatcggcga cgtcaaggac gccgcacgcc
     3481 tgctcgagcg cgcccgcgtc ctgaccgagg agccgagctt cacggacgtc gaccgcgccg
     3541 acgtgctctt ccggctcggc gcctgccgct acaacctctc gtcgatcgcg acggcggtca
     3601 gcctgctcac ccaggcgatc gagctggccg accgctccga gctgccgagc gaccggctcc
     3661 gcgcgctcgc cctcgagtgg cgctcccgct gctaccagcg gcagcgcgac tgggaggcgg
     3721 cgcgggagga cgtcgagcgc gcgctcgagc tcgcccgcgg gatcgacgac cgctacgcgc
     3781 tcgcgcaggc gcacttccag gcgtcgctcg tggccgagcg ccagggccgg tgggttctcg
     3841 cgcgcacgca cgccgagcag gccaagggcc tctacgagga gatcgcgcac caggcgaacg
     3901 tcggcgcgct cctgaacaac ctcggcggtc tcaacttcct cctcggcaag ccggaggagg
     3961 ccaaggcgta cctgaaggac gcgttccgcg tcgcgctcga ggtgggcagc gacatggagg
     4021 ccggctacgc cgtctcgtcg ctcgcgcagg tgcatctccg caccggcgag ctcgacctcg
     4081 cggacgagca ggcgcgccac gcgctcgagc tcctcaaggg gcgcgaggac gacctgcagg
     4141 acgagatcgg caacgtccag ctcgtgctcg gccgctcgct cctggagcgg ggccggctcg
     4201 acgaggcgga ggagtggttc cgccgcgcag aggcgaactt cgaccagctc tcctcggcca
     4261 gccatcgcgc cgccgcctgg atcgcgcagg gcgatctcgc ggcgatgcgc ggcgacgacc
     4321 gcagcgccgc cgatctctac cggcgcgcgg ccgaggcgct ccaggacttc cacttctaga
     4381 aaggaggtga acgcatgagg ggtcgtttga tcatgttggt tacggcgctc gtaccggtgg
     4441 cgctgttcgt gggcgaggcc tggatggcct tcccggggtt caggcgcggc gggtaacgcg
     4501 cgcgggaagg acgcgaggct ctggcggcga atctgtcacc cgccgtgcga agtcatccgc
     4561 cgctcgacaa ccgtccttct ctccgccccc tcgcgccggt tctagcaccg ctcgtagccg
     4621 tctgggccgc cgtcggcgtc ctgatcgcag cgcagctctt ctagccccgt cgcgttcgcg
     4681 cctgccggac atcccctccg gcacgctctc cccggcgtcc gctgcggtcg atctgccgcg
     4741 cgggcgccgg acgcgtttaa actgccgggg cgcatgggca agatcgacgc atacgcgaag
     4801 cccgtcctcg tcgacacgga ctggctcgcc gagcacctcg gggacgacgg cgtcgtcgtc
     4861 gcggaggttg acgagtcgcc cgagctctac gacgagggtc acatccccgg ggcggtcaag
     4921 cttcactggc aggaggacct tcaggatccg gtcgagcgcg acctcgtcgg gcgggaggac
     4981 ttcgagcggc tgctcgggtc gctcggcgtc gggtcggaca cgaccgtcgt cctctacggg
     5041 gacaagaaca actggttcgc cgcgtacgcg tactggtacc tcaaggtcta cgggcacggg
     5101 gacgtccgga tcctcgacgg cgggcgccag aagtggatcg acgagcggcg cgagctgacg
     5161 gcggacgcgc cgagccccag ggccgtggag taccacgcgg gggagcgcga cgagtcgatc
     5221 cgggcgtacc gcgaccaggt gcgcgagtgg atcggcgccg gggaccgcgc gctcgtcgac
     5281 gtccgctcgc cgggcgagta cgcgggcgac ctcgtcgcgc cgccgggcta cgagcaggag
     5341 ggcgcccagc gcgccggcca catcccgacc gccgcgtcga tcccgtgggc gagcgcggtc
     5401 gccgacgacg ggacgttccg tccggccgag gagcttcgcg acctctacgc cggcaagggc
     5461 gtgaccgccg acaaggaggt cacggcctac tgccggatcg gcgagcgctc ggcgcacacg
     5521 tggttcgtcc tccacgagct gctcggcttc gagaaggtgc gcaactacga cggctcgtgg
     5581 accgagtggg gcaatctcgt cgacgtcccg atcgagaagg gcgccggcta ggcggtcgcg
     5641 ccggtcacgc gcggaggatc gggcgagtga ggcacgtcgg ccccccgtcg cccttcttcg
     5701 agagctcctc gcccgagtag accctgacgt ccacgccggc cgcctccatg cggcggcgcg
     5761 tctccgggtt cccctcgagg gcgagcgcga cccggggcgc gagcgcgagg acgttcgggc
     5821 ccatcgacca gaactcctcg tcgggcacct cgacgaacgc gatgccgcgc tcctggagca
     5881 gctccacgag ccgcacgggc atgtacggga ggtacacgag cgcgagatcc gtgtcgagcg
     5941 gcgagacgag cgacatgagg tggagcacct cgccgggccc gttgaggtgc ggcaggtcga
     6001 acgcgatcac gtcgacgtcg gggaggagct cggctagccc ttccacgccg gcgtcgttcg
     6061 tgcggtagcc gcggccgacg aggagcgtcc gctcgtcgag ccagacggtg tcgccgccct
     6121 cggcggtcgc cggcgcctcg agccggcccc ggatcgggac gcccgcctcg acgaggtcgg
     6181 cggccatcgc gtcgatctcg gcccggcgcc ccggcttgcc gggccggagg aggagcgcgc
     6241 cctcgtccgt gacgagcacg gggtcgtacg tgtagatcgc gtccgtcgcg gcggccagcg
     6301 gcgtgccggc gacgatcacc tcggcgcccg cctcggcgag gagcgcccgg aaggcctcgt
     6361 gctcctcggc gatcttcgcc gtatcgggct cggagcgcca ctcgaacgcc tgccaggccg
     6421 cgcacccgtc ggccgtcggc gggcgcacga gcactcgccg cagcggcgcc gtcatgctct
     6481 ggcacccgta cccgagcgcg aggcggtcgg cgggctcgag cgtcgtcatg gcgcgatcgt
     6541 acgtgtcggg tggcccgccg ggacgctggt acgctcagcc gcgtggagag cgccacgcgc
     6601 ctgaggggcc tcgaggcggg cggcgggtgc gccgcgaagt acccggccgc gcggctccgg
     6661 gagctccttg ccggcttcgt gcccgccgag gcggacgacc tgctggtcgg cctcgacccg
     6721 gccgacgacg cggcggtcta ccggctcgac gacgagcgcg cgctcgtgtt cacgatcgac
     6781 ttcttcccgc cgatgctcga cgacccggcg ctctacgggg cgatcgccgc cgcgaacgcg
     6841 ctgaacgacg tcttcgccat gggcggacgt cccctcgtgg ccctctccgt cgcggcgttc
     6901 cccgaggagc tgccgatcga gaccgtccgc gccatcctcg acgccgcgtc ggcgaagacg
     6961 gaggaggcgg gggcgctgct cgccggcggg cacacgatcc gcgacgacga gccgaagtac
     7021 gggctcgcgg tcgtcggcac cgtccacccg ggcgcgctct ggcgcaagga cggggcccgt
     7081 cccggcgacg cgctcttcct gaccaagccg ctcggcacg
//
LOCUS       DASTFC010000077         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_429842,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000077 DASTFC010000000
VERSION     DASTFC010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429842"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..498)
                     /locus_tag="VFO88_02295"
                     /pseudo
     CDS             complement(1..498)
                     /locus_tag="VFO88_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(518..1189)
                     /locus_tag="VFO88_02300"
     CDS             complement(518..1189)
                     /locus_tag="VFO88_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9544385.1"
                     /translation="MPLEKLDHIALWVSDRDSIADAVTSRLGMHVIDRTDAFTLVGSN
                     ARRGKLTLFAAEGPRERGALAHVGLRVNDLAAARDALGGEEATLADGLVVRLVEGPTD
                     QDYDLDHVAIYSRDPEATAERYVDQLGFEPAEPGPGGEPRVEVGGAYVVFLEGEPGDP
                     ESPLLNHVAVLVPSADEQLAESERLGVEVADVVDAANTKAVFVWGPERVKVEYVEHKP
                     TFSLV"
     gene            1253..1600
                     /locus_tag="VFO88_02305"
     CDS             1253..1600
                     /locus_tag="VFO88_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544386.1"
                     /translation="MAAERGKILVVAARQPDFAELLEKDGFEVELRTRPLEDAGETDA
                     DVAVVFRGRLIGRSQAPALVEGGTPVVEVLTVEPPSVSTAQWIRLSNRVAKADLAQVV
                     HALVAWTRVRVHG"
     gene            1699..1839
                     /locus_tag="VFO88_02310"
     CDS             1699..1839
                     /locus_tag="VFO88_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544387.1"
                     /translation="MYPPVIQYETRRRDIEAELLRRQRRVERRGAVPARSRRRRLVAR
                     FA"
BASE COUNT          267 a          761 c          729 g          257 t
ORIGIN      
        1 ccgcagcccg tcgcccgtgc tccacgggtt cgagcgcagc gcgaggtgct ccgcctccgg
       61 cgtcacgtgc tcgcgcacga ggccgcgacc cgccgcgaag ccgccggtcg cgaggacgag
      121 cggtgcgccg tccgggagcg cggtcagcgg ctcgccgagc cggatctccc ccgcgcgcgc
      181 cgcgagcgcc tcggtcgtcc cctcgacgtc gagccggacg ccgaccgtca gcgggttccc
      241 ggtctcctcc tcgagcacgg gcgcgccgac gccgcgcagc caggcgagcg cgtcgtcgaa
      301 ccgctcccag acgagccgct ggagcgccgg gtcgccgccg gggcactccg cgcggaaccc
      361 ctcccactcc cggtagcgga agacgtagcc gccggagagc cgcatcgacc ctcccggccg
      421 gtcgcccttc tcgtggacga cgacgtccgc gccgagctcc cgcgcgcgca cggccgcgac
      481 gagcccgccg agccccgctc ccccgacgac gagcggccta gacgagcgag aacgtgggct
      541 tgtgctcgac gtactcgacc ttcacgcgct cggggcccca gacgaacacg gccttcgtgt
      601 tcgccgcgtc cacgacgtcc gcgacctcga cgccgaggcg ctcggactcc gcgagctgct
      661 cgtccgcgga cggcacgagc accgcgacgt ggttcaggag cgggctctcc gggtcgccgg
      721 gctcgccctc gaggaagacg acgtaggcgc cgcccacctc gacgcgcggc tccccgccgg
      781 ggccgggctc cgcgggctcg aacccgagct ggtcgacgta acgctccgcg gtcgcctccg
      841 ggtcgcggga gtagatcgca acgtggtcga ggtcgtagtc ctggtcggtc ggcccctcga
      901 cgagccggac gacgagcccg tcggcgagcg tcgcctcctc cccgccgagc gcgtcgcggg
      961 ccgccgcgag gtcgttcacg cgaagcccca cgtgcgcgag cgcgccgcgc tcccgcggcc
     1021 cctcggcggc gaagagcgtg agcttcccgc gccgcgcgtt cgagccgacg agcgtgaacg
     1081 cgtcggtgcg gtcgatcacg tgcatcccga gccgggacgt gacggcgtcg gcgatcgagt
     1141 cccggtccga gacccagagg gcgatgtggt cgagcttctc cagcggcatc gaggaggggt
     1201 gttcctggtc gtacgcggtt ggaaacggcg cgacggtggg gactattcca ccatggcggc
     1261 cgagaggggg aagatactgg tcgtggccgc gcggcagccg gacttcgcgg agctgctcga
     1321 gaaggacggg ttcgaggtcg agctgcgcac gcgaccgctc gaggacgcgg gtgagacgga
     1381 cgcggacgtt gccgtcgtct ttcgggggcg gctgatcggc cgctcccagg cgccggctct
     1441 cgtcgagggc gggacccccg tcgtggaggt gctgacggtc gagccgccga gcgtctcgac
     1501 ggcgcagtgg atccggctct cgaaccgcgt cgcgaaggcc gacctggcgc aggtcgtgca
     1561 cgcgctcgtc gcctggacgc gagtgcgggt tcacgggtag ttcggccagc cgcaccgacg
     1621 gacgcctgcc agcgcgattc cgccctgccg cgtgcctgct ccatcctgga cgcgttcacg
     1681 ccgtagaagg agtcacagat gtatccaccc gtcatccagt acgagacccg ccgccgcgac
     1741 atcgaggccg agctcctccg ccggcagcgt cgcgtcgagc gccgcggcgc cgttccggca
     1801 cgctcccggc ggcgccgcct ggtcgcacgc ttcgcgtagg accgtcgttt cgccggggtg
     1861 cggccacccg cacctggaaa cgccgggcag ccccattccg gcggaagggc ggtcctgcga
     1921 gccttgacgc cacagtcgag agaaggagct ggacatgcaa ccccccgtca tccattacga
     1981 ggcccgccgc cgcgagatcg aggccgacct cgcg
//
LOCUS       DASTFC010000078         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1484619,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000078 DASTFC010000000
VERSION     DASTFC010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484619"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFO88_02315"
     CDS             complement(<1..468)
                     /locus_tag="VFO88_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544388.1"
                     /translation="MSDTIRVLVVDDHAVVRSGLRLVLDAADGVETVGEAGSAEDGIR
                     AARELKPDVVLMDVVMPGMNGLEATPPLLLASPGSKVLVLSMQDDPRYVREAFAAGAS
                     GYVLKEAADADVVEAVREVASGGRYVHPALGARLATAEMEATARAEADPLSERE"
     gene            complement(465..2099)
                     /locus_tag="VFO88_02320"
     CDS             complement(465..2099)
                     /locus_tag="VFO88_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9544389.1"
                     /translation="MRTEQERLRAVVEAGIALTSELSLDTLLQRIVETASRLTGARYA
                     ALGVLDAGRVSLERFVYTGIDDEMRARIGDLPRGRGILGALIEDAKPLRLHAIADDPR
                     SVGFPEHHPPMQTFLGVPVTIRGIAYGNLYLTEKEGGEDFTDEDEDVALLLAAQAGTA
                     IENARLYESATRWSRQLETLNEIIAAMASEVELPRLLNLVAARLREVVNARLVTIALP
                     TEGKDLRVEAADGEGAAVLLGARLGHDSSKHARVLERGRTERVDSFLDDPEVDQALTR
                     QAGLRSGLYVPLTIGERRIGVVAAHDKLTTDGRFTDADVRLSEIFAARAAVAVDLSRR
                     VAQDALERAIAAQETERRRLALELHDETGQELTSILLGLRGVEEAQTDEERASAIAEV
                     RALAVQTLQNVRRLAVELRPKALDDFGLVPALERLAESFGERTGMKVEVESAGLDARL
                     PELVETTLYRVVQEALTNVVKHAQAGHVSIVLARRGSAATAVVEDDGRGFDPASENGG
                     IGLAGMRERLALIGGRLEVESGPGVGTTIVAEAPLP"
BASE COUNT          281 a          837 c          797 g          299 t
ORIGIN      
        1 ctcccgctcg ctcagggggt cggcctccgc gcgcgcggtc gcctccatct ccgccgtcgc
       61 gaggcgcgcg ccgagcgccg ggtggacgta gcgcccgccc gaggccacct cgcgcaccgc
      121 ctcgacgacg tccgcgtcgg ccgcctcctt gaggacgtag ccggacgcgc ccgccgcaaa
      181 cgcctcgcgg acgtagcgcg ggtcgtcctg catcgagagg acgagcacct tggagccggg
      241 cgaggcgagc agaagcggcg gcgtggcctc gagcccgttc atgccgggca tgacgacgtc
      301 catgaggacg acgtccggct tgagctcgcg ggcggcgcgg atcccgtcct ccgccgaccc
      361 cgcctcgccg accgtctcga caccgtcggc cgcgtcgagc acgagccgca ggccggagcg
      421 cacgacggca tggtcgtcga cgacgaggac gcgaatcgtg tcgctcacgg gagcggcgcc
      481 tcggcgacga tcgtggttcc cacgccgggc cccgactcga cctcgagccg cccgccgatg
      541 agcgcgagcc gctcgcgcat tcccgcgagc ccgatcccgc cgttctcgga ggccgggtcg
      601 aacccgcgcc cgtcgtcctc cacgaccgcg gtcgccgcgc ttccgcgtcg cgcgagcacg
      661 atcgacacgt ggccggcctg cgcgtgcttc acgacattcg tgagcgcctc ctgcaccacg
      721 cggtagagcg tcgtctcgac caactccggg agccgggcat cgagccctgc cgactcgacc
      781 tcgaccttca tccccgtgcg ttcgccgaac gactcggcga gccgctcgag cgcggggacg
      841 agcccgaagt cgtcgagggc cttcgggcga agctcgaccg cgaggcggcg gacgttctgg
      901 agcgtctgga cggcgagggc gcgcacctcg gcgatcgcgc tcgcccgctc ctcgtccgtc
      961 tgcgcctcct ccacgccgcg caggccgagg aggatcgagg tcagctcctg tcccgtctcg
     1021 tcgtggagct cgagcgcgag ccgtcggcgc tccgtttcct gcgccgcgat cgcgcgctcg
     1081 agcgcgtcct gcgcgacgcg ccgcgacagg tcgaccgcga cggcggcgcg cgcggcgaag
     1141 atctcgctca gccgcacgtc ggcgtcggtg aagcggccgt cggtcgtcag cttgtcgtgg
     1201 gcggcgacga cgccgatccg ccgctcgccg atcgtcagcg ggacgtagag ccccgagcgg
     1261 aggccggcct ggcgggtgag cgcctggtcg acctcggggt cgtcgagaaa cgagtcgacc
     1321 cgctcggtcc gaccgcgctc cagcacgcgc gcgtgcttcg aggagtcgtg cccgagccgc
     1381 gcgccgagca ggacggccgc gccctcgccg tccgcggcct cgacgcgcag atccttcccc
     1441 tccgtcggga gcgcgatcgt cacgagccgg gcgttcacga cctcgcggag ccgcgccgcg
     1501 acgaggttca agagccgcgg cagctcgacc tcgctcgcca tcgcggcgat gatctcgttc
     1561 agggtctcga gctggcgcga ccagcgcgtg gcggactcgt agagccgcgc gttctcgatc
     1621 gccgtccccg cctgcgcggc gaggaggagc gccacgtcct cgtcctcgtc ggtgaagtcc
     1681 tcgccgccct ccttctccgt caggtagagg ttgccgtagg cgatgccgcg gatcgtgacc
     1741 gggacgccga ggaaggtctg catcggcgga tggtgctcgg ggaagccgac ggagcgcggg
     1801 tcgtccgcga tcgcgtggag gcgcagcggc ttcgcgtcct cgatcagggc gccgaggatc
     1861 ccgcgccccc gcgggaggtc gccgatccgc gcgcgcatct cgtcgtcgat gccggtgtag
     1921 acgaagcgct cgagcgagac gcggccggcg tcgaggacgc cgagcgccgc gtagcgcgcg
     1981 ccggtgagcc ggctcgccgt ctcgacgatc cgctggagga gcgtgtcgag cgagagctcg
     2041 gaggtcagcg cgatgccggc ctcgacgacc gcgcgcaggc gctcttgctc cgtcctcacg
     2101 cggctgatta tgacgacggc tctcgcggaa atgccgagcc gcccgcttct agcgcgcgat
     2161 ccggcggcgc acctcgtccg gcacgaaccg ctcgtccatg agcacgagcg cgcc
//
LOCUS       DASTFC010000079         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_627741,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000079 DASTFC010000000
VERSION     DASTFC010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627741"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1062
                     /locus_tag="VFO88_02325"
     CDS             181..1062
                     /locus_tag="VFO88_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544390.1"
                     /translation="MRLLVATRTGITALDDGRAEDVWEGQVRCLAREGLRVYAGTASG
                     AVRSVDDGATWAPSGLAGSRVMSLAVDGESVLAGAQPVALHRSDDGGRTWRTLDSFPR
                     RAWWWQPATKPHTQGYVSALATRGDTILAGIEAFRGYRSGDRGGSWRPLRRGFSRDCH
                     ALVLAHGRAYEGAGLGPSWSLDGGHAWEPLRAGLDRRYVIAIAVDPADADCWYAAAAP
                     LLKAHSRNSQARIFRWSDGRWAPVTEELRELPHALVCPQPDAVVAGLRDGTLRSSADR
                     GETWETLARVDGVRALV"
     gene            1123..>2423
                     /locus_tag="VFO88_02330"
     CDS             1123..>2423
                     /locus_tag="VFO88_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9544391.1"
                     /translation="MVGADMQAPGPRLRLAELLASVSLATDLGTGQPPGHALRTCAVA
                     TALAEALGLGPAEVRAVHQVALLRFLGCTADSAETAAAVGGDDRAFLAAMAPAWMGST
                     REATGRFVRAVGAGEPWPRRVALVARGLADPNEAERSLSAHCEVAVMLAARMGLEQPV
                     VDALAHAYERWDGKGHPAGLAGEEIPVAVRVVVVARDVDLALKQGEDPRALARARRGR
                     AYDPAVVDALDDVAPDVLAGLEGADEWEAALAAEPLPAATVAAGELDDVLSAWADFAD
                     LKSPWTRGHSRAVASLAEAAGRRAGLDAARCDGLRLAGLVHDIGRVAVENGIWDKPGA
                     LTTSEWERVRLHPYFTERVLVRCTALAPIAGPAASHHERTDGSGYHRSLGGDALSPAD
                     RILAAADVFAALVADRPHRPALGPDDAARTLEAEAGRGLDA"
BASE COUNT          253 a          907 c          951 g          312 t
ORIGIN      
        1 gacgcgggct tcgacggccg gatcgcccgc gtggcgagca aggactcgtt catcccgctc
       61 ggcgacgcgt cgagcctcgt gctcctctcc gaggaggaga tcgaggcggc ggcgctggcg
      121 ctcgtgggcg agatccgaag cgagcgcgat cgctcggcgc ggtcggcgta gactcggcgc
      181 atgcggcttc tcgtcgcgac gcggacgggg atcaccgccc tcgacgacgg tcgcgcggag
      241 gacgtctggg aaggtcaggt ccgttgcctc gcgcgcgagg gactccgcgt ctacgccggc
      301 acggcatcgg gagccgtccg ctcggtcgac gacggtgcga cctgggcgcc ctccggcctc
      361 gccggaagcc gggtcatgtc gctcgcggtg gatggcgaga gcgtcctcgc cggcgcgcag
      421 cccgtggcgc tccaccggtc ggacgacggc ggccgaacct ggcgcaccct cgactcgttt
      481 ccgcgccggg catggtggtg gcagccggcg acgaagccgc atacgcaggg ctacgtctcg
      541 gccctggcga cgcggggcga cacgatcctc gcaggcatcg aggcgttccg cggctaccgg
      601 tcgggcgacc gcggagggtc gtggcgcccg ctccggcgcg gcttctctcg cgactgccac
      661 gcgctcgtgc tggcgcacgg acgcgcatac gaaggtgccg gccttggccc ctcttggagc
      721 cttgacgggg gtcatgcctg ggagccgctg cgagcgggtc tcgaccggag gtacgtgatc
      781 gcgatcgccg tggaccccgc cgacgcggac tgctggtacg ccgccgctgc gcccctgctc
      841 aaggcgcact cacgcaactc gcaggcgcgc atcttccgct ggtcggacgg gcgctgggcg
      901 ccggtgaccg aggagctccg ggagcttccg cacgcgctcg tctgcccgca acccgacgcc
      961 gtcgtcgccg ggctccggga cgggacgctt cgctcgtccg ccgaccgcgg cgagacctgg
     1021 gagacgctgg cccgcgtcga cggcgtccgg gctctcgtct gaagcccggc ggccgcggtg
     1081 tcggcgtcgt ctccggcccg cgcgccggtt agcgtaggcg cgatggtcgg cgcggacatg
     1141 caggcgccgg gaccgcggct ccggctcgcc gagctcctcg cgtccgtctc gctcgcgacg
     1201 gacctcggca ccggccagcc gcccgggcac gcgttgcgga cgtgtgcggt cgcgaccgcg
     1261 ctcgccgagg ccctcggcct cggcccggcg gaggtccgcg cggtccacca ggtcgcgctg
     1321 ctccggttcc tcggctgcac ggccgactcg gccgagaccg cggccgccgt cggcggcgac
     1381 gaccgcgcgt tcctcgccgc gatggcgccg gcgtggatgg gctcgacgcg cgaggcgacc
     1441 gggcgcttcg tgcgtgcggt cggggccggc gagccctggc cgcgccgtgt cgcgctcgtc
     1501 gcacgggggc tcgccgaccc gaacgaggcg gagcgctcgc tctcggcgca ctgcgaggtc
     1561 gccgtgatgc tcgcggcgcg gatgggcctc gagcagccgg tcgtcgacgc cctcgcgcac
     1621 gcgtacgagc gctgggacgg caagggccat ccggcggggc tcgcgggcga ggagatcccg
     1681 gtggccgtgc gcgtcgtcgt cgtggcgcgc gacgtcgacc tcgcgctcaa gcagggcgag
     1741 gacccgcgcg cgttggcgcg tgcgcggcgc gggcgggcct acgaccctgc cgtggtcgac
     1801 gcgctcgacg atgtggcgcc cgacgtcctc gccggcctcg agggcgcgga cgagtgggag
     1861 gcggcgctcg cggccgagcc gctgccggcc gccacggtcg cggccggcga gctcgacgac
     1921 gtcctctcgg cgtgggccga cttcgccgac ctgaagtcgc cctggacgcg cggccattcg
     1981 cgcgccgtcg catcgcttgc ggaggcggcg gggcgccggg cgggcctcga cgccgccagg
     2041 tgcgacgggc tccgcctcgc cgggctcgtg cacgacatcg gccgggtggc ggtcgagaac
     2101 gggatctggg acaagccggg tgcgctgacg acctccgagt gggagcgcgt gcggctccac
     2161 ccgtacttca ccgagcgcgt cctcgtgcgc tgcacggcgc tcgcgccgat cgcggggccc
     2221 gccgcctcgc accacgagcg gacggacggg tccggctacc accgctcgct cggcggcgac
     2281 gcgctctcgc cggccgaccg gatcctcgcg gccgccgacg tgttcgcggc gctcgtcgcc
     2341 gaccggccgc accgtccggc gctcgggccc gacgacgcgg cccggacgct cgaggcggag
     2401 gccgggcgtg gcctcgacgc gga
//
LOCUS       DASTFC010000080         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_809040,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000080 DASTFC010000000
VERSION     DASTFC010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809040"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFO88_02335"
     CDS             complement(<1..182)
                     /locus_tag="VFO88_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544392.1"
                     /translation="MSKRTLARVGGLAAFLAGVFWGFTLPLIATQATDDPVGLRYDDF
                     NRWLTLPLALLLVTLVV"
     gene            complement(203..1021)
                     /locus_tag="VFO88_02340"
     CDS             complement(203..1021)
                     /locus_tag="VFO88_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET9544393.1"
                     /translation="MPPSTVCLTFDFDALSVWLAYEKTTPAMLGRGEYGARVGVPRVL
                     DLLASHGLEATFFVPGHTVESFPDETERILAAGHEVAHHSYAHLDPSTQVRDEEREDM
                     ERAWASLARIGVEPLGFRAPSADLSASTLELVEEHGFLYDSSLMADDYTPFRPRIGDR
                     VARGEPLVRGREARFWELPLSFELDDWVHFQFNFDPYRRGGSTPGDVLEIWTAEFDWM
                     HANVDGGVLTATMHPQVIGRGSRIAMLDRFIRHCGEAGARFARMDTVARELDGA"
     gene            complement(1021..2388)
                     /locus_tag="VFO88_02345"
     CDS             complement(1021..2388)
                     /locus_tag="VFO88_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET9544394.1"
                     /translation="MASERYGSRLGARAASLPPAATDAIRGDPADDLAPPVEPNLVRP
                     REIPGARRVGRNTVEILLFRGLSTPLALGLVVLQSRFLEPEGRGAYVVAVLGVTIVSR
                     LLGQLGVAVSNRLRTEGDELRGLVHRALALGIGGGLLAVPAIAAAGAGIGGIDLDVAL
                     VAAFALAPNVVWQSVSGVLLGQARVRLWNYVQLGSPVLTLAGMLVLVVALDLGVLGAV
                     TAWLVANVLTAAFALGAARDLWTPVALPRLDPVARSLAVLAAGMGAVHVLNLLSYRIE
                     LFILDRHAGLAEIGIYSVAMQAAEAMWLLSAAVANAVTGPAIHDDEPGAVRLVRRAAL
                     RALGLTAAVAAVVGLAAPFVLPAILGEAFEGSVRPLLLLLPGIVLYAPVSVLVVYLSV
                     RHGRPRLSVLVSLLGMAVTLGLSLVLIPEHGVSGAAVASALGYAAGGALAWLFFVRVA
                     GRRLG"
     gene            2387..>3361
                     /locus_tag="VFO88_02350"
     CDS             2387..>3361
                     /locus_tag="VFO88_02350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9544395.1"
                     /translation="MPIRESPAPSVRPSLPPWWLDEALAAEGEAPEAPPLEGEERADV
                     AIVGGGYTGLWAALALREREPSARVAVLEAEICGWGPSGRNGGFLNGYWTHLSRLRPV
                     FGDEAAVAIGRAAAQVVPAVRAFCEARGEDVWLREGGYLKVSAAPAQDAAVDKAIRTA
                     EELGVPEEARPLSREQVAERISSPVFRRGVLFRDGSTVQPARLARALRRAVLADGIAL
                     HERTRVTRVRAGAPSTLETPRGRLVADEVIVATNAALAGWRPLRRFLTNFGSYVVLTE
                     PVPELVEEIGWTGGEAVADGRMFVHYFRTTNDGRVLMGSGSGPIGHGGR"
BASE COUNT          478 a         1233 c         1277 g          373 t
ORIGIN      
        1 acgacgagcg tcacgagcag gagcgcgagc ggcagcgtga gccaccggtt gaagtcgtcg
       61 tagcggaggc cgaccgggtc gtccgtcgcc tgcgtcgcga tgagcgggag cgtgaagccc
      121 cagaagactc cggccaggaa cgcggcgagg ccgccgaccc gcgcgagcgt ccgcttcgac
      181 acacgcggag cgtaacggag cctcaggccc cgtcgagctc gcgcgcgacg gtgtccatcc
      241 gggcgaagcg cgcccctgcc tcgccgcagt ggcggatgaa ccggtcgagc atcgcgatcc
      301 ggctcccgcg gccgatcacc tgcgggtgca tcgtcgccgt cagcacgccg ccgtcgacgt
      361 tcgcgtgcat ccagtcgaac tccgccgtcc agatctcgag cacgtcgccg ggcgtcgagc
      421 cgccgcgccg gtacgggtcg aagttgaact ggaagtggac ccagtcgtcg agctcgaagg
      481 agagcggcag ctcccagaag cgcgcctcgc gcccgcggac gagcggctcc ccgcgcgcga
      541 cacggtcgcc gatccgcggc cggaacggcg tgtagtcgtc cgccatgagc gacgagtcgt
      601 aaaggaagcc gtgctcctcc acgagctcga gcgtcgatgc ggagaggtcg gccgacggcg
      661 cccggaagcc gagcggctcg acgccgatcc gcgcgagcga ggcccaggcg cgctccatgt
      721 cctcgcgctc ctcgtcgcgc acctgtgtgc tcgggtcgag gtgcgcgtac gagtggtgcg
      781 cgacttcgtg cccggcggcg aggatccgtt ccgtctcgtc tgggaacgac tcgaccgtgt
      841 ggccggggac gaagaacgtc gcctcgagcc cgtgcgaagc gagcaggtcg agtacccgcg
      901 gcaccccgac ccgcgccccg tactcgccgc gcccgagcat cgccggagtc gtcttctcgt
      961 acgcgagcca gacgctgagc gcgtcgaagt cgaacgtgag gcagacggtg ctcggcggca
     1021 tcacccgagt ctacggccgg ccacgcggac gaagaagagc cacgcgagcg ccccgccggc
     1081 cgcgtagccg agcgcgctcg cgaccgccgc gcccgagacg ccgtgctcgg ggatgaggac
     1141 gagcgagagc ccgagcgtca ccgccatccc gaggagcgag acgagcacgc tcagacgcgg
     1201 ccggccgtgg cgaacggaga ggtagacgac gagcaccgac accggcgcgt agagcacgat
     1261 ccccggcagg agcaggagca gcggtcgcac ggagccctcg aacgcctcgc cgaggatcgc
     1321 cggcaggacg aacggcgcgg cgagcccgac gacggccgcg accgccgcgg tcagcccgag
     1381 cgcgcggagc gccgcgcgcc gcacgagccg caccgctccg ggctcgtcgt cgtggatcgc
     1441 gggccccgtc accgcgttcg ccacggcggc cgagaggagc cacatcgcct ccgccgcctg
     1501 catggcgacg gagtagatgc cgatctcggc gagccccgcg tggcggtcga ggatgaagag
     1561 ctcgatccgg tagctcagga ggttgagcac atggacggcg cccatcccgg ccgcgagcac
     1621 cgcgagcgag cgcgcgaccg ggtccaggcg cggcagcgcg accggcgtcc agaggtcgcg
     1681 cgcggcgccg agcgcgaagg cggccgtgag gacgttcgcg acgagccatg cggtcaccgc
     1741 gccgagcacg ccgaggtcga gcgcgacgac gagcacgagc atcccggcga gcgtcagcac
     1801 gggcgagccg agctgcacgt agttccagag gcgcacgcgc gcctgcccga gcaggacgcc
     1861 ggacacggac tgccacacga cgttcggcgc gagcgcgaag gcggcgacga gggcgacgtc
     1921 gaggtcgatc ccgccgattc cggctccggc ggccgcgatc gccggcacgg cgaggagccc
     1981 gccgccgatc ccgagcgcga gcgcgcggtg cacgagcccg cgaagctcgt cgccctcggt
     2041 ccgcagccgg ttcgagacgg cgacgccgag ctggccgagc agccgcgaga cgatcgtcac
     2101 gccgagcacc gcgacgacgt acgcgccgcg cccctccggc tcgaggaagc ggctctgcag
     2161 gacgacgagc ccgagggcga gcggcgtcga gagcccgcgg aagaggagga tctcgaccgt
     2221 gttccgtccg acgcgccgcg ctcccgggat ctcgcgcggg cggacgaggt tcggctcgac
     2281 gggcggcgcg aggtcgtccg ccgggtcgcc gcggatcgcg tccgtcgcag cgggcggcag
     2341 ggatgcggcg cgcgcgccga ggcggcttcc gtaacgttcg ctcgccatgc cgatccggga
     2401 gtctcccgca ccgagcgtcc gtccgtcgct tccgccctgg tggctcgacg aggcgctcgc
     2461 cgccgagggc gaggcgcccg aggcgccgcc gctcgaaggg gaggagcgcg ccgacgtcgc
     2521 gatcgtcggc ggcggctaca cggggctctg ggcggcgctc gctctcaggg agcgcgagcc
     2581 gtcggcccgc gtcgccgtcc tcgaggcgga gatctgcggc tggggcccga gcgggcgcaa
     2641 cggcggcttc ctgaacggct actggacgca cctctcgcgt ctgcgtcccg tgttcggcga
     2701 cgaggcggcc gtcgcgatcg ggcgcgcggc ggcgcaggtc gtcccggccg tgcgcgcgtt
     2761 ctgcgaggca cgcggggagg acgtctggct ccgcgaaggg ggctacctca aggtctccgc
     2821 cgcgcccgcg caggacgccg cggtcgacaa ggcgatccgg acggccgagg agctcggcgt
     2881 tccggaggag gcgcggccgc tctcgcggga gcaggtcgcg gagcggatct cctcgcccgt
     2941 cttccggcgg ggcgtcctgt tccgcgacgg ctcgaccgtg cagcccgccc ggctcgcccg
     3001 ggcgctgcgc cgcgccgtcc tcgccgacgg gatcgcgctc cacgagcgca cgcgggtcac
     3061 ccgcgtccgc gccggggcgc cctcgacgct cgagacgccg cgtggccgcc tcgtcgccga
     3121 cgaggtgatc gtcgccacga acgccgcgct cgccggctgg cgcccgctcc gccggttcct
     3181 gaccaacttc ggctcctatg tcgtgctcac ggagcccgtc cccgagctcg tcgaggagat
     3241 cggctggacc ggcggcgagg cggtcgccga cgggcggatg ttcgtccact acttccgcac
     3301 gacgaacgac gggcgcgtgc tcatgggctc gggctccggg ccgatcggcc acggcgggcg
     3361 g
//
LOCUS       DASTFC010000081         6175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_166296,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000081 DASTFC010000000
VERSION     DASTFC010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166296"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..975
                     /locus_tag="VFO88_02355"
     CDS             481..975
                     /locus_tag="VFO88_02355"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HET9544396.1"
                     /translation="MRIEEPQELFEHKLCAALKMESTAAEMLATLTEKTQSPELRGVL
                     LRHADETQHQIVNLHEVFAAIGADPKEKASAAIKGIETEGQTLIRMTDESLVDAVVLA
                     SAAETEHHEIAVYESLIAYAEALGHGTVVGLLRENLRQEERMLEEVKAANERLAGRVA
                     AGVV"
     gene            1179..1249
                     /locus_tag="VFO88_02360"
     tRNA            1179..1249
                     /locus_tag="VFO88_02360"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1211..1213,aa:Cys,seq:gca)
     gene            1348..2430
                     /locus_tag="VFO88_02365"
     CDS             1348..2430
                     /locus_tag="VFO88_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET9544397.1"
                     /translation="MAATLTVPAAGEAASIYVTRGVVVYSAGAGEENAVELTVTETFD
                     PEFFELNMDVTVRDNGALIVAGTGCTALAPSEALCQVSHAASGWADWFAAYLRGEADT
                     FTAAGERTDVGNGVTVFGGAGADELEGTEEADTLRGGPGDDMLDGRAGTVPLDKFILG
                     GDRLFGGAGADLLSGGDGGHDELRGGLGADTLRGGRGPNDVVSYQGLTDRVVVRLNGR
                     RDDGRPGERDLVARDVEDVMSGSGNDLLIGNDRGNWLAGGPGDDALIARGGRDLLEGD
                     PGGDSLRGGFGHDRIYGDSWHPRGGWWPSRVGRDTALGGAGNDRFEMLDRKRDRVVGG
                     TGRDRARVDAGRDLLFQIELILDAAF"
     gene            complement(2499..2723)
                     /locus_tag="VFO88_02370"
     CDS             complement(2499..2723)
                     /locus_tag="VFO88_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544398.1"
                     /translation="MPRCEGYYTLETSRCPNEGDHEMKAADGDHYALCEYHADAVWTT
                     TVARWHGETDLRASASVELQAQAREPSFVW"
     gene            2816..3673
                     /gene="folD"
                     /locus_tag="VFO88_02375"
     CDS             2816..3673
                     /gene="folD"
                     /locus_tag="VFO88_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET9544399.1"
                     /translation="MAATLMDGKGLAERVRAEVKEEVAALGRPVGLATVLVGDDQASA
                     IYVRRKREACAEVGIESFHHELGADTGEDELLGLVADLNADERVTGILVQLPLPGQID
                     EDRVIQAIDPVKDVDGFHPSNAGLLFQGQPTLVAATPSGIMAILAEYDVPLQGARALV
                     IGRSNIVGKPIALLLLAEHATVTIAHSRTRDLDALAREADVLVAAVGRADTVTKDMVK
                     PGATVIDVGINRVDGKLVGDVSPDVAEVAGLLTPVPGGVGPMTIASLLRNTVKAARYQ
                     SGALAFPGA"
     gene            3746..3952
                     /locus_tag="VFO88_02380"
     CDS             3746..3952
                     /locus_tag="VFO88_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET9544400.1"
                     /translation="MAEGTVKWFSNEKGYGFISRDGGDDVFVHHSKIEMDGYRSLVEG
                     QRVQFEVEEGPKGLQAASVQLLQE"
     gene            4051..4335
                     /gene="gatC"
                     /locus_tag="VFO88_02385"
     CDS             4051..4335
                     /gene="gatC"
                     /locus_tag="VFO88_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET9544401.1"
                     /translation="MAITREDVLHVARLARLEIPEEEIGRVQEQLAAILEAVGKVSEL
                     DLSDVEPTSHPLDVVNAWAEDEPRPSLPQDEALANAPDHEDGHFRVPAVG"
     gene            4457..5896
                     /gene="gatA"
                     /locus_tag="VFO88_02390"
     CDS             4457..5896
                     /gene="gatA"
                     /locus_tag="VFO88_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET9544402.1"
                     /translation="MSGDTAGIDTLRLTAEEAAGLLERGEVSSAELVAAYRAAIEARD
                     PELHAYLHVTDDEHAGDGGGVPIALKDVITTKGVPTTAGSRILEGYVPVFDSTVAARC
                     KEAGLPLLGKTNLDEFAMGSSTENSGFGPTRNPWDPARVPGGSSGGSAAVVAAGLAPW
                     SLGTDTGGSVKQPASLCGVVGLRPTWGTVSRYGIVAFASSLDQVGPLAKTVRDCARLY
                     GITAGRDACDSTTVELPAPVELPEREDLRGLRVGVPRELNEVDGIEPGVRERVQEAIR
                     LAEELGAEVGETTLPRSVEYGLACYYLVAPAEASANLARYDGVRYGHRAAGDGDLTAL
                     YERTRWEGFGAEPKRRIMLGTYALSAGYYDAYYGQAQKVRTVIKQEFAAAFEDFDVLL
                     SPTSPTVAFPLGDKVENPLAMYLTDVLTIPPNMAGLPGLSIPCGLSEGLPVGLQILGP
                     QFSENTLFTVGHALERALGFDFVPPRLAS"
     gene            5893..>6175
                     /locus_tag="VFO88_02395"
     CDS             5893..>6175
                     /locus_tag="VFO88_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544403.1"
                     /translation="MRELLDRLRERARAPKADNPAGVEAVLRETFRFLEEHQGYELAQ
                     AKTLPDGAVAAYENRAAHRAIAVFARRDRGAWAGVTRIDEGAPLPPVNRD"
BASE COUNT          892 a         2135 c         2268 g          880 t
ORIGIN      
        1 ccgtgaggac gtagggattc ccggaggagc tcgtcctgct gatctggccc gaatcgagca
       61 gtgagacgac tgcgaccgag ttgttgttcg cgacgaggcc cacgagaccg gggaacgtca
      121 ggatgtaggc tccggtgcca accttgttca cggtgggcgc tccgcccctc cggttgaacc
      181 acttgatgac cgtcccgtcg gccccgatgc cggggtggcc gccatgacca cgccgatcgc
      241 gatgcccctg gcgaccagcg cgatgatgcg cccgtcttct gtccctgcac ctgcgctccc
      301 ttcgttgacg gttcgccggc agacgtcgtg tgggccgtgc cacccggagc gccaagtaaa
      361 gggatcgcgt gggctggact caatccccag gtcgaggatc ggttaccggc gcgaggcggg
      421 gggtagggcg ccagtgacca ggaccgacgc gaagatcggg ggcatttgat gctcgaagcg
      481 atgaggatcg aggagccgca ggagctcttc gagcacaagc tctgcgcggc gctcaagatg
      541 gagtcgacgg ccgccgagat gctcgccacg ctgacggaga agacgcaaag cccggagctc
      601 cgcggggtcc tcctccgcca cgccgacgag acccagcacc agatcgtcaa cctccacgag
      661 gtgttcgccg cgatcggcgc cgacccgaag gagaaggcgt ccgccgcgat caaggggatc
      721 gagaccgagg gccagacgct catccgcatg acggacgagt cgctcgtcga cgccgtcgtc
      781 cttgcgagcg ccgccgagac cgagcaccac gagatcgccg tctacgagag cctcatcgcg
      841 tacgcggagg cgctcgggca cggaacggtc gtcgggctct tgcgcgagaa cctccggcag
      901 gaggagcgga tgctcgagga ggtcaaggcc gcgaacgagc gcctcgcagg gcgggtcgcg
      961 gccggggtcg tgtagccgct gcggcgtggt gccgcggagg ccgcctgctc ggcggccccc
     1021 gcggatccca ccaggaggga ccccgatgcg gatccggggc cggcgccggc cgtgcaactc
     1081 gccccgggac aaggcggacc gaccggctcc gcgccgggac ttcgtcgcat ccgtggcaat
     1141 tcctgccggt tcgccgccgc cgctacgatc cgtcgagcgg cgcggtggcc gagtggttag
     1201 gcagaggcct gcaaagcctc gtacaccggt tcaaatccgg tccgcgcctc tcgcgttgac
     1261 gtgcgtccac gggccgaggc ttggcgcgac gctccggacg cgcgacgatc gtggtctgtg
     1321 cgtcttgtca cgttcccgct cctactggtg gccgcgaccc tcacggttcc cgcggccggc
     1381 gaagctgcct ccatctacgt gactcgcggg gtcgtggtgt actcggccgg cgcgggagag
     1441 gagaacgcgg tcgagctcac ggtcacggaa acgtttgatc cggagttctt cgagctgaac
     1501 atggacgtca cggtgcgcga caacggtgct ttgatcgtcg ccgggacggg ctgtaccgcc
     1561 cttgccccga gcgaggccct atgccaagtc tcgcacgctg catctggatg ggcggactgg
     1621 tttgccgcct atctgcgtgg cgaagcggac actttcactg cggccggcga gcgaacggac
     1681 gtgggcaacg gcgtcaccgt cttcggcggg gcgggcgctg acgagctcga gggcacggag
     1741 gaggccgaca ccctgcgagg cggtcccggt gatgacatgc tcgacggccg tgcgggcacc
     1801 gtcccgctcg acaagttcat cttgggaggc gatcgtcttt ttgggggcgc cggcgccgac
     1861 ctcctctcgg gcggtgacgg gggacacgac gagctgcgcg gcggtcttgg agcggacacg
     1921 ctgcggggtg gccgtgggcc gaacgacgtg gtctcctacc aggggctcac ggatcgcgtc
     1981 gtcgtgcggc tcaacgggcg acgcgacgac ggacgtccgg gcgagcgtga tctggttgcg
     2041 cgcgacgtcg aggacgtcat gtccggtagc ggcaacgacc tcttgatcgg caacgaccgg
     2101 ggcaactggc tcgccggtgg gccgggcgac gacgcgctga tcgcgcgcgg cgggcgcgat
     2161 ctactcgagg gtgacccggg aggggattct ctccgcggtg gattcggcca cgatcggatc
     2221 tacggcgact cctggcaccc ccgtggtgga tggtggccct cgcgagtcgg ccgcgacacg
     2281 gcgttgggcg gcgcgggcaa cgatcgcttc gagatgctcg accggaagcg cgaccgggtt
     2341 gtgggaggga cgggtcgcga cagagcgcga gtcgacgcag ggcgcgatct gctcttccag
     2401 atcgagctca tcctggacgc cgcgttctga gctcagggtt gggcgagctc gtcatccacg
     2461 cgccgcgtac ggcgcagggc tgatcccggc gtgggcggct accagacgaa ggaaggctcg
     2521 cgggcctgag cctggagctc gacggaggcc gacgcgcgga ggtcggtctc gccgtgccag
     2581 cgtgccaccg tcgtcgtcca cacggcgtcc gcgtggtact cgcagagcgc gtagtggtct
     2641 ccgtcggcgg ccttcatctc gtgatcgccc tcgttcgggc agcggctggt ctcgagggtg
     2701 tagtacccct cacatcgcgg catggcctca gcgtggctcg ttcgagggac gctgtcgatc
     2761 atccgaacgg gggaggggcg tccggcacgg gtccgcttct gtagattccc cggccgtggc
     2821 tgccacgctc atggacggga aggggctcgc cgagcgcgtc cgcgccgagg tgaaggagga
     2881 ggtcgccgcg ctcgggcggc cggtcgggct cgcgacggtg ctcgtcggcg acgaccaggc
     2941 gtccgcgatc tacgtgcgcc gcaagcggga ggcgtgcgcc gaggtgggga tcgagagctt
     3001 ccaccacgag ctcggcgccg acacgggcga ggacgagctc ctcggcctcg tcgccgatct
     3061 gaacgcggac gagcgcgtga ccgggatcct cgttcagctc ccgctgccgg ggcagatcga
     3121 cgaggaccgc gtcatccagg cgatcgaccc ggtgaaggac gtggacgggt tccacccgtc
     3181 gaacgcgggc ctcctcttcc agggccagcc gacgctcgtg gcggccacgc cctcggggat
     3241 catggcgatc ctcgccgagt acgacgtgcc gctccagggc gcccgcgcgc tcgtgatcgg
     3301 gcggtcgaac atcgtcggga agccgatcgc gctgctcctc ctcgccgagc acgcgacggt
     3361 gacgatcgcg cacagccgta cgcgcgacct cgacgcgctc gcgcgcgagg ccgacgtgct
     3421 cgtcgccgcg gtcggccgcg cggacacggt cacgaaggac atggtgaagc ccggcgcaac
     3481 cgtgatcgac gtcgggatca accgagtgga cggcaagctc gtgggcgacg tgagcccgga
     3541 cgtcgccgag gtcgcgggcc tcctgacgcc cgtcccgggc ggggtcggcc cgatgacgat
     3601 cgcctcgctc ctgcgcaaca cggtcaaggc ggcgcggtac cagtccgggg cgcttgcctt
     3661 tcccggcgcc tgagcgctac agtccgacca gttgcaacat ggcggcggcc ctgatcgggc
     3721 cgtcgttcgc gtagaaggag gaacgttggc tgagggcacc gtcaagtggt tctcgaacga
     3781 gaagggctac ggcttcatct cgcgggacgg aggcgacgac gtcttcgtcc accactcgaa
     3841 gatcgagatg gacgggtatc gctccctcgt cgaggggcag cgcgtccagt tcgaggtcga
     3901 ggaggggccg aagggcctcc aggcggcgag cgtccagctc ctgcaggagt agcccggcgt
     3961 ctcgtgaccg acttggcggg ccccggacga cggggcccgt cctttttcac cggcgggcga
     4021 ccccgtccgc gtccctagac tggaaggccg atggcgatca cccgtgaaga cgtgctgcac
     4081 gtcgcgcgcc tcgcgcggct cgagatcccg gaggaggaga tcgggcgcgt gcaggagcag
     4141 ctcgcggcga tcctcgaggc ggtcggcaag gtgtccgagc tcgacctctc ggacgtggag
     4201 ccgacctcgc acccgctcga cgtcgtgaac gcctgggccg aggacgagcc gcgcccgtcg
     4261 ctcccgcagg acgaggcgct cgcgaacgcc ccggaccacg aggacggcca cttccgcgtg
     4321 cccgcggtcg ggtgaggatc gtgacggtct cggcacgctt tcggcacacc gccttcacca
     4381 tgcgcggacc tgcgcgggtt acggtgtcga cgggggagac gctaatcggg gggcccccgg
     4441 gggagggcgt accggcgtga gcggggacac ggccggaatc gacacgctgc gcctcaccgc
     4501 ggaggaggcg gccgggctgc tcgagcgcgg cgaggtgtcg agcgcggagc tcgtcgccgc
     4561 ctaccgcgcc gcgatcgagg cgcgcgaccc ggagctccac gcctacctcc acgtgaccga
     4621 cgacgagcac gcgggcgacg gcggcggcgt gccgatcgcg ctcaaggacg tgatcacgac
     4681 gaagggcgtc ccgacgacgg ccggctcgcg gatcctcgag gggtacgtcc ccgtcttcga
     4741 ctcgaccgtc gccgcgcgct gcaaggaggc gggcctgccg ctcctcggca agacgaacct
     4801 cgacgagttc gcgatgggct cctcgacgga gaactcgggc ttcggcccga cgcgcaaccc
     4861 gtgggacccg gcccgcgtcc cgggcggctc ctcgggcggc agcgccgccg tcgtcgcggc
     4921 cgggctcgcg ccctggtcgc tcggcaccga cacgggcggc tctgtgaagc agccggcgtc
     4981 gctctgcggc gtcgtcggcc tgcgcccgac gtggggcacc gtctcgcgct acgggatcgt
     5041 cgccttcgcg tcgagcctcg atcaggtcgg cccgctggcg aagaccgtcc gcgactgcgc
     5101 gcggctctac gggatcaccg ccggccgcga cgcatgcgac tcgacgaccg tcgagctgcc
     5161 ggcgcccgtc gagctgcccg agcgcgagga cctccgcggc ctgcgcgtcg gcgtcccccg
     5221 cgagctgaac gaggtcgacg ggatcgagcc cggcgtgcgc gagagggtcc aggaggcgat
     5281 ccggctcgcc gaggagctcg gcgccgaggt cggcgagacg acgcttccgc gctccgtcga
     5341 gtacgggctc gcctgttact acctcgtcgc ccccgcggag gcgagcgcga acctcgcgcg
     5401 ctacgacggc gtccgctacg ggcaccgggc cgcaggcgac ggcgacctga ccgccctcta
     5461 cgagcgcacc cgctgggagg gcttcggcgc cgagccgaag cgccgcatca tgctcggcac
     5521 gtacgcgctc tcggccggct actacgacgc gtactacggc caggcgcaga aggtgcgcac
     5581 ggtgatcaag caggagttcg ccgccgcctt cgaggacttc gacgtcctgc tcagcccgac
     5641 ctcgccgacc gtcgcgttcc cgctcgggga caaggtggag aacccgctcg ccatgtacct
     5701 gaccgacgtc ctcacgatcc cgccgaacat ggccgggctg cccggcctct cgataccgtg
     5761 cggcctctcc gagggcctcc cggtcgggct ccagatcctc gggccgcagt tttccgagaa
     5821 cacgctcttc accgtcggcc acgcgctcga gcgcgcgctc ggcttcgact tcgtcccgcc
     5881 gaggctcgcg tcgtgaggga gctgctcgac cgcctgcgcg agcgtgcccg cgcgccgaag
     5941 gccgacaacc cggccggcgt cgaggccgtg ctccgcgaga cgtttcgctt cctcgaggag
     6001 caccagggct acgagctcgc gcaggcgaag acgctgccgg acggcgccgt cgccgcgtac
     6061 gagaaccggg ccgcgcaccg ggcgatcgcc gtcttcgcgc ggcgcgaccg cggcgcctgg
     6121 gccggcgtca cgcgaatcga cgagggtgcg ccgctcccgc ccgtgaaccg cgaca
//
LOCUS       DASTFC010000082         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_248666,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000082 DASTFC010000000
VERSION     DASTFC010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248666"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1149)
                     /locus_tag="VFO88_02400"
     CDS             complement(70..1149)
                     /locus_tag="VFO88_02400"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9544404.1"
                     /translation="MQALVVTPGEPHSVRVAEHPVEAADGAVLVRTLEVGVCGTDREI
                     SEGIFGVAPGDERALVLGHELLGVVERDGHGFSRGDLVTATVRRSCRRCVACEEGAPD
                     SCLTGDYSERGITRLHGFARELVAEDPAQLVPIPRALGRLGVLAEPASICARALRHAR
                     AIGHRQPWRAERALVIGAGAIGMLVTYLLRLDGLEVWTAALDAPGSPRAGLVEASGAR
                     YVPSRETPLEDLRDDVGGFDLVVEAAGDAQLMAATLGLLRRSGVACLLGIDPRERTVE
                     LDGPTIGLDAVLENRVLFGSVNAHREDWVAAVEGLARARERWPEALERFVTLRVPLDR
                     FEEAFEHRGVKATLVLGETGSIPLA"
     gene            complement(1150..1881)
                     /locus_tag="VFO88_02405"
     CDS             complement(1150..1881)
                     /locus_tag="VFO88_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9544405.1"
                     /translation="MAEAEFAGKRALVTGAGSGIGAAVARRLAAGGASVVLADVRRDG
                     IEALGEELGGDAQPLLLDVRDEEAVAPAVAGLDVLANVAGIGSTQSAPDTPLEVWEDV
                     FAVNARGTFLTCKHAIPDMAARGGGAIVNVASVAALVGLRNRAAYCASKGAVVALTRA
                     LAVDHVGVGIRVNAVCPGTVDSPWVRRLVEEVGESLDALRARQPLGRLGTPEEIAEAV
                     VYLASDRAGFVTGSVLTIDGGLTAA"
     gene            1901..2815
                     /locus_tag="VFO88_02410"
     CDS             1901..2815
                     /locus_tag="VFO88_02410"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9544406.1"
                     /translation="MDVALRLEATLGEGPRWDAAGRRLLAVDIEGRRLHRFDPATGED
                     RPLDLGERIGVALPVEGGGLLLALARRLAVLDEAANRMETLAAFPHGDDVRANDGACD
                     PAGRLFVGTMALDERPGGGALYRYTAEAGLERVLDAVTLSNGLGWSPDGTRMYYVDSP
                     TRRVDVFDYDAATGEPGARRPFARIAAEDGIPDGLAVDDEGGVWVALYGGGAVCRFDP
                     DGRLDRVLEVPATNVTACCFGGPDGRSLFVTTARQGLGETELEREPLAGSIFFADAGV
                     AGPPATPFAHGGGTRSTAPSEADETSAR"
     gene            complement(2755..4500)
                     /locus_tag="VFO88_02415"
     CDS             complement(2755..4500)
                     /locus_tag="VFO88_02415"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9544407.1"
                     /translation="MNGSGASAERGARPAGRPGSQDDLRRRARAGLAGAPERAPARAH
                     FRALGIDPARLDGPIVGVVSTWTETMPCNLNQRDLAEHVKAAVEAAGGVALAFTTIAV
                     SDNQSQGTPGMRASLVSREVIADSIELMTHAHDFDALVCLVGCDKTVPGALMALARVD
                     KPAVCLYSGPMRAGRWRGREVAIGDVWEAVGAEERGLLSRAELDELERVACPGAGTCA
                     GHFTANTMAVALELLGIAALGDGLIPAAELAEKRAAAGRAGGLAVRLAGESVTARRFL
                     SRGALLNAMAGIAATGGSTNGLLHLLALAREAGVALELDELAAVGARTPVLASLAPGG
                     RWMAEDFHRAGGTATLVRELVRGGLVDGGAPTVTGDALGDAPAGAREPDGEVLAPVER
                     PFSAGGALRVLRGSLAPEGAVVKLAGIERRAQRGPARVFESEEACAEAVRGGGVRAGD
                     VLVVRYEGPAGGPGMREMLSVTASVVGAGLGESVALVTDGRFSGATRGLMVGHVSPEA
                     ARGGPLAIVRDGDPVAIDADAGTLELELPAAEIAARLAEWRPPALPYADGVFGRYRAL
                     VSSASEGAVLRVPPP"
     gene            4681..>5618
                     /locus_tag="VFO88_02420"
     CDS             4681..>5618
                     /locus_tag="VFO88_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9544408.1"
                     /translation="MTRGAAAAASPAWRTRVAAVPPRTLAVRLLIGLVLAFVAVYVGY
                     KAADDPRTFVVVGLNGLTLAGLYFVVASGFTLIFGLMRVVNMAHGSLYMLAGFFALEL
                     QKNLTGGEATSAFGTSRAGVGDWIIPLVVACLAVGLIGLVMQQVFLRWNQGQELRQAL
                     ITIAISIIIADQVLANFGGVAENLAAPDSFPETVSLRLYDISYPFFRIFVLLVAIAVG
                     LALWAMIKRTRFGMIVRAGVDDRQMVSALGINIQLVFASAFLIGAFLAGLGGVLGGTM
                     ISVNKGNDTTYLLFSLIVVIIGGMGSLAGAAIGALAL"
BASE COUNT          695 a         2198 c         2020 g          705 t
ORIGIN      
        1 ggtcgagatt cgcgagaccg ggacgttcga ggcgctctag gagggaccgg cggcgtcgag
       61 gccgtgcgcc taggcgagcg ggatcgagcc cgtttcgccg aggacgagcg tcgccttgac
      121 gccgcggtgc tcgaacgcct cttcgaaccg gtcgagcggc acgcgcaggg tgacgaagcg
      181 ctcgagcgcc tccggccagc gctcgcgcgc ccgcgcgagg ccctcgaccg cggcgaccca
      241 gtcctcgcgg tgcgcgttga cgctcccgaa gaggacacgg ttctcgagga cggcgtcgag
      301 gccgatcgtg gggccgtcga gctcgacggt ccgctcgcgc gggtcgatcc cgagcaggca
      361 ggcgacaccg cttcggcgca gcaggcccag ggtcgcggcc atgagctgcg cgtcgccggc
      421 cgcctcgacg acgaggtcga agccgccgac gtcgtcccgg aggtcttcga gcggcgtctc
      481 ccggctcggg acgtagcggg cgccggaggc ctcgacgagc cccgcgcgcg ggctgccggg
      541 cgcgtcgaga gcggccgtcc agacctcgag gccgtcgagg cggaggagat aggtgacgag
      601 catcccgatc gcaccggcgc cgatcacgag cgcacgctcg gcccgccacg gctgcctgtg
      661 cccgatcgcc cgcgcatgcc tgagcgcacg cgcgcagatc gaggccggct ccgcgagcac
      721 gccgaggcgg ccgagcgcgc gcgggatcgg cacgagctgc gccggatcct cggcgacgag
      781 ctcgcgcgcg aacccgtgca ggcgcgtgat cccccgctcg ctgtagtccc cggtcaggca
      841 cgagtccggg gcaccctcct cgcacgcgac gcagcgccgg cacgagcgcc ggaccgtcgc
      901 cgtgacgaga tccccccgcg agaacccgtg gccgtcgcgc tcgacgacgc cgagcagctc
      961 gtggccgagg acgagcgccc gctcgtcgcc cggcgcgacg ccgaagatgc cctcggagat
     1021 ctcccggtcg gtgccgcaca cgccgacctc gagcgtccgc acgagcaccg cgccgtccgc
     1081 ggcctcgacc gggtgctcgg cgacccgaac ggagtgcggc tcgcccgggg tgacgacgag
     1141 cgcctgcatt caggccgccg tgagcccgcc gtcgatcgtg aggacgctcc ccgtcacgaa
     1201 ccccgcccgg tcggacgcga ggtagacgac cgcctcggcg atctcctccg gcgtgccgag
     1261 ccgcccgagc ggctggcgcg cgcgcagcgc gtcgagcgac tcgccgacct cctcgacgag
     1321 gcgccgcacc cacggcgagt cgaccgtgcc cgggcagacc gcgttcacgc ggatccccac
     1381 gccgacgtgg tcgacggcga gcgcgcgggt cagcgccacg acggctccct tcgaggcgca
     1441 gtaggcggct cggttgcgca ggccgacgag ggccgcgacc gacgcgacgt tcacgatcgc
     1501 gccgccgccg cgggccgcca tgtcggggat cgcgtgcttg cacgtgagaa acgtcccgcg
     1561 cgcgttgacg gcgaagacgt cctcccacac ctcgagcggc gtgtcgggcg cgctctgcgt
     1621 cgagccgatc ccggcgacgt tcgcgaggac gtccaggcct gccaccgccg gcgcgacggc
     1681 ctcctcgtcg cgcacgtcga gaagaagcgg ctgcgcgtcg ccgccgagct cctcgccgag
     1741 cgcctcgatc ccgtcccgcc ggacgtccgc gaggacgacg gacgcgccgc ccgccgcgag
     1801 ccgccgcgcg accgcggcgc cgattcccga gccggccccg gtcacgagcg cccgctttcc
     1861 cgcgaactcc gcctcagcca tggccgctta accttcccgg atggacgtcg ccctgcgcct
     1921 cgaagcgacg ctcggcgagg gcccgcgctg ggacgcggcc ggccggcggc tcctcgccgt
     1981 cgacatcgag ggacgcaggc tccaccgctt cgaccccgcg acgggcgagg accgcccgct
     2041 cgacctcggc gagcggatcg gggtcgcgct gcccgtggag ggcggcgggc tcctgctcgc
     2101 gctcgcacgc cggctcgccg tcctcgacga ggcggcgaac aggatggaga cgctcgccgc
     2161 gttcccgcac ggcgacgacg tccgtgcgaa cgacggcgcg tgcgacccgg ccgggcggct
     2221 cttcgtcggg acgatggcgc tcgacgagcg gccgggcggc ggcgcgctct accggtacac
     2281 ggccgaggcc gggctcgagc gcgtgctcga cgccgtgacg ctctcgaacg ggctcggctg
     2341 gtcgcccgac gggacgcgga tgtactacgt cgactcgccg acgcgccggg tggacgtgtt
     2401 cgactacgac gccgccaccg gcgagcccgg tgcgcggcgc ccgttcgcgc ggatcgccgc
     2461 cgaggacggg atccccgacg ggctcgcggt cgacgacgag ggcggcgtct gggtcgcgct
     2521 ctacggcggc ggcgcggtgt gccggttcga tccggacggc cggctcgacc gggtgctcga
     2581 ggttcccgcg acgaacgtga ccgcgtgctg cttcggcggt ccggacgggc gctcgctctt
     2641 cgtcacgacg gcgcggcagg gcctcggcga gaccgagctt gagcgcgagc cgctcgcggg
     2701 cagcatcttc ttcgccgacg ccggcgtcgc cgggccgccc gccacgccct tcgctcacgg
     2761 cggcggcacg cggagcaccg cgccctccga ggcggacgag acgagcgcgc ggtagcgccc
     2821 gaagacgccg tccgcgtacg gaagggcggg cgggcgccac tccgcgagcc gcgcggcgat
     2881 ctcggcggcc gggagctcga gctcgagcgt ccccgcgtcg gcgtcgatcg cgaccgggtc
     2941 gccgtcccgc acgatcgcga gcggcccgcc gcgcgccgcc tcgggtgaga cgtgcccgac
     3001 catgagcccg cgcgtcgcgc cggagaagcg cccgtcggtg acgagcgcga ccgactcgcc
     3061 gaggcctgcg ccgacgacgg aggcggtgac gctcagcatc tcgcgcatgc cgggcccgcc
     3121 cgccggcccc tcgtagcgga cgacgagcac gtcgcccgcg cgcacccctc cgccgcggac
     3181 ggcctcggcg cacgcctcct cgctctcgaa gacgcgcgcc ggcccgcgct gggcgcggcg
     3241 ctcgatcccg gcgagcttca cgaccgcgcc ctccggcgcg aggctcccgc gcaggacgcg
     3301 gagcgccccg ccggcggaga acggccgctc gaccggggcg agcacctcgc cgtccggctc
     3361 gcgcgcgccc gccggcgcgt ccccgagcgc gtcgcccgtc accgtcggcg caccgccgtc
     3421 cacgagcccg ccgcggacga gctcccgcac cagggtcgcg gtcccgccgg cgcgatggaa
     3481 gtcctccgcc atccaccgcc cgccgggcgc gaggctcgcg agaacgggcg tcctcgcgcc
     3541 gaccgcggcg agctcgtcga gctcgagcgc gacgccggcc tcccgggcga gcgcgagcag
     3601 gtggaggagg ccgttcgtcg agccgcccgt cgcggcgatc cccgccatcg cgttcaggag
     3661 cgcgccgcgc gagaggaagc gccgcgcggt cacgctctcg cccgcaagcc gaacggcgag
     3721 cccgcccgcc cgcccggcgg cggcgcgctt ctccgcgagc tccgcggcgg ggatcagccc
     3781 gtcgccgagc gcggcgatcc cgaggagctc gagcgcgacg gccatcgtgt tcgcggtgaa
     3841 gtgccccgcg cacgtcccgg cccccgggca ggcgacgcgc tcgagctcgt cgagctccgc
     3901 gcgcgagaga aggccgcgct cctcggcgcc gaccgcctcc cacacgtcgc cgatcgcgac
     3961 ctcgcgcccc cgccagcggc ccgcgcgcat cggccccgag tagaggcaga ccgcgggctt
     4021 gtccacgcgg gcgagcgcca tgagcgctcc gggcaccgtc ttgtcgcagc cgacgaggca
     4081 gacgagcgcg tcgaagtcgt gcgcgtgcgt catgagctcg atcgagtccg cgatcacctc
     4141 gcgcgagacg agcgaggcgc gcatccccgg cgtcccctgc gactggttgt ccgagaccgc
     4201 gatcgtggtg aacgcgagcg ccacgccgcc ggccgcctcg accgccgcct tcacgtgctc
     4261 ggccaggtcg cgctggttca ggttgcacgg catggtctcc gtccacgtcg agacgacgcc
     4321 gacgatcggg ccgtccaggc gcgccgggtc gatcccgagc gcgcggaagt gcgcccgcgc
     4381 cggagcccgc tccggcgcgc cggcgagccc cgccctggcc cggcggcgga ggtcgtcctg
     4441 cgagccaggc cggccggccg gtcgagcgcc tcgctcggcg ctggctccag atccgttcat
     4501 gcgattacgg tactccgacc caccgcgctc tcgcgggcgc ggcccgcgcg ctcctttgac
     4561 ccacgtgcgc cgaagcgggc gctttgcaac ccggcctccg cagtggtacg gtcgcccttc
     4621 aaaagtgttc gtctcttttc aaattcgatc atccgcctgc ctttctagag agcggctcgt
     4681 gtgacccggg gggcggcggc ggcggcgagc ccggcgtggc ggacgcgcgt agcggccgta
     4741 ccgccgagaa cgctcgcggt acgcctcctc atcggcctcg tcctcgcctt cgtcgcggtc
     4801 tacgtcggct acaaggccgc cgacgacccg cggacgttcg tcgtcgtcgg cctcaacggg
     4861 ctcacgctcg cgggcctcta cttcgtcgtc gcgagcggct tcacgctcat cttcggcctc
     4921 atgcgcgtgg tgaacatggc gcacggctcg ctctacatgc tggcgggctt cttcgcactc
     4981 gagctccaga agaacctcac cggcggggag gccacgtcgg ccttcggcac ctcgcgggcc
     5041 ggggtcggcg actggatcat cccgctcgtc gtggcgtgcc tcgccgtcgg tctgatcggc
     5101 ctcgtcatgc agcaggtctt tctgcgctgg aaccagggtc aagagctgcg ccaggccctg
     5161 atcacgatcg ccatctcgat catcatcgcc gaccaggtcc tcgccaactt cggcggagtc
     5221 gccgagaacc tcgccgcccc cgactccttc ccggagacgg tgagcctccg cctctacgac
     5281 atctcctatc ccttcttccg gatcttcgtc ctgctcgtcg cgatcgccgt cgggctggcc
     5341 ctctgggcca tgatcaagcg cacgcgcttc gggatgatcg tgcgcgcggg cgtggacgac
     5401 cggcagatgg tctccgcgct cgggatcaac atccagctcg tcttcgcgag cgcgttcctc
     5461 atcggcgcgt tcctcgccgg cctgggcggc gtcctcggcg ggacgatgat ctcggtcaac
     5521 aaggggaacg acacgaccta cctgctcttc tcgctcatcg tcgtcatcat cggcggcatg
     5581 ggcagcctcg ccggggccgc gatcggcgcc ctcgccct
//
LOCUS       DASTFC010000083        11130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1303354,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000083 DASTFC010000000
VERSION     DASTFC010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303354"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..872
                     /locus_tag="VFO88_02425"
     CDS             72..872
                     /locus_tag="VFO88_02425"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9544409.1"
                     /translation="MPGRIFALANQKGGVGKTTTAVNLAACLAEAGERTLVVDLDPQA
                     NATSGLGERANGASSGDLLDGAELRQIVRRTRFANLDLVPAKPELAGLAVELARHADG
                     ETFLAESLAGARDEYSFVFVDCPPSLGPLTVSALAAADQVIVPIQCEYYALEGLTQLL
                     DSIERLRARLNPRLSVAGMILTMADGRTRLAAEVAAEVRRHFGDLVFDAVVPRSVRLA
                     EAPSHGLPITAYDPTSAGADAYYRVAGELVDRLRGAPSSRESLVHAGR"
     gene            853..1707
                     /locus_tag="VFO88_02430"
     CDS             853..1707
                     /locus_tag="VFO88_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9544410.1"
                     /translation="MSTPAADPRRRGLGRGLEVLLGGTPPEAELARLPLGQIKPNPRQ
                     PRRRVDAEALGGLAESVRAQGVVQPIIVRPVGAAAYEIIAGERRWRAARAAGLATVPA
                     VVRETDDRESLVLALVENVARENLTAVEEARAYALLQDEFGLSLGEVAERVGRSKPSV
                     SNRLRLLELPDDVLDCVERGQLSEGHARAVLSIPGNEERRALVRRIIRDGLSVRAAER
                     AARWAGARTRPRRSAPVDPELAARARAALERLTGAPVKVGARRIEIPFDGEVELAELV
                     ESLERARA"
     gene            1739..1993
                     /locus_tag="VFO88_02435"
     CDS             1739..1993
                     /locus_tag="VFO88_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544411.1"
                     /translation="MLGSLLTELETLDALDRCHETRAVVLRMARLASHGRLGAFVDAV
                     GADSKLDERTRRWVLDLARHEPFLLATAEYASLQSASAGD"
     gene            1984..2057
                     /locus_tag="VFO88_02440"
     tRNA            1984..2057
                     /locus_tag="VFO88_02440"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2018..2020,aa:Ile,seq:gat)
     gene            2156..3604
                     /locus_tag="VFO88_02445"
     CDS             2156..3604
                     /locus_tag="VFO88_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544412.1"
                     /translation="MYRTVLFLSLLALGFVGPALASGDQEAGLPDVWVGCPYGDVDPD
                     GNPVCLEEVPAEDPFEGALARAVATAEAQEAAANCRLKNEVFFWTDTNWLRVARGLAA
                     NPAPCTEYYISVPPMSNQKTEPRRNQAAQIREFGPQFHALFDIFLADATGWVNWVAEN
                     RHRFGSEEETWYEAGVEARRRMALPDRDFDVGSGDSWLVNDFNTGTRRDGGTYFGLDL
                     EPWKRANMRALVRGLHEGPADGSLGKTRGLAQVAIQFTHQNIPFVDEYKADWKAWLED
                     EAFWADIAEHVRWIGKEVYADSRYWGTPDTSRNERSRHLQQYFFHVPELAEAGPESVS
                     VARGYLRESFLPLVNGTWAALGPDRFTPLFCCGHGETMLPIERFLTYVTEELFAVRQY
                     AGSHPQGAPAGRLGFTWQPTNNFGVPNAEFLAAQDAIVARIAESVRWGYRQGGASPAG
                     ICSPPASEVDWCTGGREGALFTDAWRAFESWD"
     gene            complement(3662..4057)
                     /locus_tag="VFO88_02450"
     CDS             complement(3662..4057)
                     /locus_tag="VFO88_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544413.1"
                     /translation="MKSRHLIVALAAGIAAATSLAFAAPASGGGDREVRVRGTCTGAA
                     TSKLKLSEENGRIEVEFEVDQNVNGRRWRVTLKRNGVRVFRGIRTTQPPSGSFEVRRL
                     VADRAGRETITARARALSGGQLCTARARW"
     gene            4208..4705
                     /locus_tag="VFO88_02455"
     CDS             4208..4705
                     /locus_tag="VFO88_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET9544414.1"
                     /translation="MEEMVIYGVSFDMVGKQPIVLLKTADGNKFLPIWIGHPEAAAIL
                     MKLQGASTPRPMTHDLFTDMLAQLDARVVRIAVTELRDNTFYALVTLAVNGSEIEIDS
                     RPSDAIALAVRADAPIFAAESVIEESAIEFEGEEVNEEQVVEEFKQFLDEVKPEDFSA
                     EADPD"
     gene            complement(4709..4990)
                     /locus_tag="VFO88_02460"
     CDS             complement(4709..4990)
                     /locus_tag="VFO88_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET9544415.1"
                     /translation="MPPDDASTDPLVRQLREQISDNDRAIVDAINKRLKLVARLKEYK
                     ASRGFEFVDPDREDWMIRYLERANGGPLSDDGLRELFAQILALTKREVS"
     gene            complement(5001..6143)
                     /locus_tag="VFO88_02465"
     CDS             complement(5001..6143)
                     /locus_tag="VFO88_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9544416.1"
                     /translation="MRRVVAIGFLALALPAAAHAQTLTLDAAPASTKFGGTVTFSGTL
                     TPATEGVAVAVFSLAPGVSLVGQTTVAADGSWSVPAALTRPGPYLARAQLDATTTVDS
                     PQLVVPLKPRLTAEVVGKRTVGARVVLRGRMQPAAAGRLTLRFAGKARRVRVGGVGRF
                     QVTLPTDVPGRFRWSLRVAPKPGYLAARRANTLVLTAPPLALGARGQAVLALERRLNR
                     LRYALATVNSTYGYDTVEAVFALQKVHGLPRTGRVGAQEWRLLRTSGVPRARVPRGDH
                     IEVSKTTQVMYEVRDGKVVKVVHVSTGATGNTPVGKFRVYLLTPGTLPSGMYYSLFFL
                     RGFAIHGYPSVPTYPASHGCVRTPMWFAPGFYRRWGKIGTVIHVFA"
     gene            complement(6184..6933)
                     /locus_tag="VFO88_02470"
     CDS             complement(6184..6933)
                     /locus_tag="VFO88_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9544417.1"
                     /translation="MDETSSPTSPERSSDSRRGLAIVAWGVLAALQIALAFMIMLLVD
                     EDEVGRDGLYEYGTAIGNAFVYLVLLGLTVAIAGIAGRPSVVLGLRSFAWRWVGAAIG
                     IGIGVAALSYLIAALLGLDAGSEQGILPEDWRPERAGAIAANAVVIVVAAPLVEELFF
                     RGAGVGLLQSIGAVTAMVVTGVAFGLAHGLLIGLLPLAVFGIGLAWVRLRSTSVWPCV
                     IGHALYNGAVLSIGLLCLSDAECRATLGCVM"
     gene            complement(6884..8047)
                     /locus_tag="VFO88_02475"
     CDS             complement(6884..8047)
                     /locus_tag="VFO88_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9544418.1"
                     /translation="MTDERPGISTRAIHGRGRPHAPGEPLAPPIVTSTTFSFETAAEM
                     GRVMSEEEYGFTYSRLRNPTVEELNGAVAELEGAEAAQAFGSGMAAIAAALLTSLSPG
                     DTLLCARQLYGSTYAFVESQVRRLNVDVHYLDVTDLEAWERPAAVRYVETLANPGFPL
                     ADLEALAATKGDGLLIVDNTFASPVLCRPLELGADLVCESATKYLNGHHDVTAGVVAG
                     STELVARVRTFQIDTGAILDAFPAYLLRRGLKTLALRVERQSANALELARFLEGHPKV
                     ERVYYVGLASSPQHELALRQLADSGGMLAFDVPGGREAGERLMDALELAARATSLGGV
                     DTVVHHPASTSHRQFDAEQLAAAGISEGTIRVSVGCEDGRDVVADFARALERL"
     gene            complement(8044..8688)
                     /locus_tag="VFO88_02480"
     CDS             complement(8044..8688)
                     /locus_tag="VFO88_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9544419.1"
                     /translation="MRSEPPRAVTIDAFGTLVELVDPVEPLRAALAAAGVERDDEVVA
                     RAFAAEVAHYVPRAHEGRDAASLAALRRDCAAVFLAEAGADLDPAGFAPAFVRALAFR
                     PLPGTVEALDALRARGLRLACVSNWDVSLADALAAAGLAARLDAIVSSAEAGAPKPDP
                     RPFLLALERLGAEPGETLHVGDDEADRLGAEAAGLAFAPPPLSTLPARMAKASG"
     gene            complement(8678..9574)
                     /locus_tag="VFO88_02485"
     CDS             complement(8678..9574)
                     /locus_tag="VFO88_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9544420.1"
                     /translation="MRSRAPALLGLLLVLVAATGCGERDEPLGEDVDAYPVLVGDARG
                     EEIELEGRPERIVALAPSVAELAGELGAGERVVGVPAGVTVRGALGAPRVTRPSGFVD
                     IGAVARLEPDLVLAGSGAPAGDLETIARRTGAPVYVQPDRSIRDVVRAVHDLGFLLGE
                     PARARVFAASLREELAAVEEAVRGRAPVRVFVDLGLLIAPSPDSLVVDLVRRAGGQPV
                     GTGNDGAPAEPCQVLALRPEVVLRIREPVAALPDTRLVCPRRPNVDVTVVRIPGDLAV
                     RPGPRVGEALDTIARALHPDAF"
     gene            9639..10388
                     /locus_tag="VFO88_02490"
     CDS             9639..10388
                     /locus_tag="VFO88_02490"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET9544421.1"
                     /translation="MDSQDWKRVAVAGALILIAIVVARLADRAITRRIELTPEQLTRY
                     RVLRRSVTATIVAIGVLSALLVIPEIRAAAGGVLASSALLGLVIGLAARTTIANFVAG
                     VLLAFTQPLRIGDEVEVAGAQGTVEEISLTYTTIRTPGGARFFVPNEKLASETIRNAT
                     AGTAEHLARVTVPVPLASDLDRALALVEEEARTASHVEEKRAIATISQFEPAGQAILT
                     VEAWAPRGQVAAVETEIRQAVHRRLRAEGVF"
     gene            10388..>11130
                     /locus_tag="VFO88_02495"
     CDS             10388..>11130
                     /locus_tag="VFO88_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HET9544422.1"
                     /translation="MPPAGRKRPNGNGNGAARARNGNGGTRPPTRGRRERARRRRAAR
                     GKRRAIVFLVLVGAVSALAAIAAGVFTGASAALACDLSSLRPVAIGQNSFIYAADGSL
                     LGSIPAERNRTPRALDEISPLVQEAAVAIEDRRFYDHMGVDVEGIVRAAVKNLEAGEI
                     VEGGSTITQQLVRNLYIGNEKSFNRKAKEACLALKLDAEWTKERILETYLNQVYFGNH
                     AYGIEAAAQTYFSKPAEELGLAQAALLAGL"
BASE COUNT         1540 a         4040 c         4149 g         1401 t
ORIGIN      
        1 acgccggagc gcttcccgcg ccgcccgggc gcggccagga aacgtccact cgcctgagta
       61 gccttggccg cgtgccggga cggatcttcg cgctcgcgaa ccagaagggc ggggtcggca
      121 agacgacgac cgcggtgaac ctcgccgcct gcctcgcgga ggcgggcgag cggacgctcg
      181 tggtcgatct cgacccgcag gcgaacgcga cctccggcct cggcgagcgc gcgaacgggg
      241 cctcgagcgg cgatctcctc gacggcgcgg agctgcgcca gatcgtcagg cggacgcgtt
      301 tcgcgaacct cgatctcgtg ccggcgaagc ccgagctcgc ggggctcgcg gtcgagctcg
      361 cgcgccacgc cgacggcgag acgtttctcg ccgagtcgct cgcgggcgcg cgcgacgagt
      421 actcgttcgt cttcgtcgac tgcccgccct ccctcggccc gcttaccgtt agcgccctcg
      481 cggccgccga ccaggtgatc gtgccgatcc agtgcgagta ctacgccctc gaggggctca
      541 cccagctcct cgactcgatc gagcgcctgc gcgcgcggct gaacccgcgg ctctccgtgg
      601 ccgggatgat cctgacgatg gccgacgggc gcacccggct cgcggccgag gtcgccgcgg
      661 aggtgcgccg ccacttcggc gacctcgtct tcgacgccgt cgtcccacga tcggtgcggc
      721 tcgcggaggc gccgagccac ggcctgccga tcaccgcgta cgacccgacc tcggccgggg
      781 cggacgcgta ctaccgtgtc gccggggagc tcgtcgaccg gctccgcggc gcgccgtcct
      841 cgagggagag ccttgtccac gccggccgct gacccccgcc ggcgcgggct cgggcgcggg
      901 ctcgaggtcc tcctgggcgg cacgccgccg gaggcggagc tcgcgcgcct tccgctcggg
      961 cagatcaagc cgaacccgcg tcagccccgt cgccgcgtgg acgccgaggc gctcggcggg
     1021 ctcgcggagt cggtgcgcgc gcagggcgtc gtccagccga tcatcgtgag gccggtcggc
     1081 gcggcggcgt acgagatcat cgccggggag cggcgctggc gggcggcgcg cgcggccggc
     1141 ctcgcgaccg tccccgccgt ggtgcgcgag acggacgacc gcgagtcgct cgtcctcgcg
     1201 ctcgtcgaga acgtcgcgcg cgagaacctg acggcggtcg aggaggcgcg cgcctacgcg
     1261 ctcctccagg acgagttcgg gctctcgctc ggcgaggtcg ccgagcgcgt gggccgctcg
     1321 aagccctccg tgtcgaaccg gctccgcctg ctcgagctgc cggacgacgt cctcgactgc
     1381 gtcgagcgcg ggcagctctc cgagggtcat gcccgcgccg tgctctcgat cccaggcaac
     1441 gaggagcggc gcgcgctcgt gcgcaggatc atccgcgacg gcctctccgt ccgcgccgcc
     1501 gagcgcgccg cgcgctgggc cggcgcacgg acgaggccgc gccgctccgc gcccgtcgac
     1561 ccggagctcg ccgcccgggc ccgcgcggcg ctcgaacggc tgaccggcgc gcccgtcaag
     1621 gtcggcgccc gccgaatcga gatcccgttc gacggcgagg tcgagctcgc cgagctcgtc
     1681 gagtcgctcg agcgcgcccg ggcgtaaccg atcgtcccgc tttcccgttg agcgggtggt
     1741 gctggggagc ctgctgaccg agctcgagac gctcgacgcg ctcgaccgct gccacgagac
     1801 gcgcgcggtc gtcctgcgca tggcgcgcct cgcctcgcac ggccggctcg gcgcgttcgt
     1861 cgacgccgtc ggcgcggact cgaagctcga cgagcggacg cgccgctggg tgctcgacct
     1921 cgcccgccac gagccgttcc tcctcgcgac ggcggagtac gcctcgctac aatccgcctc
     1981 cgcgggcgat tagctcagtc ggttagagcg ccgctctgat aaggcggagg tccctggttc
     2041 gaatccagga tcgcccatct gctcccgccc gttgatggac atcaacgagg cacctcttgt
     2101 ctactgtgta gctagctgcg actatgcgcg cacggtctag agaagggagc cgcacatgta
     2161 ccggaccgtg ctctttctgt cgttgctcgc gctcggcttc gtcggcccgg cgctggcctc
     2221 gggcgaccag gaggcagggt tgcccgacgt ctgggtgggc tgtccgtatg gggacgtcga
     2281 cccggacggg aatccagtct gtctcgagga ggtgcccgcc gaggatccgt tcgagggggc
     2341 gctcgcacgc gccgtcgcga ccgccgaggc gcaggaggcg gccgcgaact gccgcctgaa
     2401 gaacgaggtc ttcttctgga cggacacgaa ctggctgcgc gtcgcgcgcg gcctcgcggc
     2461 gaaccccgcg ccctgcaccg agtactacat ctcggttccg ccgatgtcga accagaagac
     2521 ggagccgcgc cggaaccagg ccgcccagat ccgtgagttc ggcccgcagt tccacgcgct
     2581 cttcgacatc ttcctcgccg acgcgaccgg ctgggtgaac tgggtcgcgg agaaccggca
     2641 tcggttcggt tcggaggagg aaacctggta cgaggccggc gtcgaggcac ggcgccggat
     2701 ggccctgccc gaccgggact tcgacgtcgg aagcggcgac tcgtggctcg tgaacgactt
     2761 caacacgggg acgcggcgag acggcgggac gtacttcggg ctcgacctcg agccgtggaa
     2821 gcgcgcgaac atgcgcgccc tcgtccgcgg gctgcacgag gggccggccg acggcagcct
     2881 ggggaagacc cgcggcctcg cccaggtggc gatccagttc acgcaccaga acatcccgtt
     2941 cgtcgacgag tacaaggccg actggaaggc gtggctcgag gacgaggcgt tctgggcgga
     3001 catcgcggag cacgtccgct ggatcggcaa ggaggtctac gcggactcgc gctactgggg
     3061 cacgccggac acctcgcgca acgagcggag ccggcacctc cagcagtact tcttccacgt
     3121 gccggagctc gcggaggccg ggccggagtc cgtttcggtc gcgcgcggct acctccgcga
     3181 gtcgttcctg ccgctcgtga acgggacgtg ggccgcgctc gggccggacc ggttcacgcc
     3241 gctcttctgc tgcgggcacg gcgagacgat gctgccgatc gagcgcttcc tgacgtacgt
     3301 gacggaggag ctcttcgcgg tccggcagta cgccgggagc cacccgcagg gcgcgcctgc
     3361 cgggcggctc gggttcacct ggcagccgac gaacaacttc ggcgttccga acgccgagtt
     3421 cctcgccgcc caggacgcca tcgtcgcgcg gatcgccgag tccgtccgct gggggtaccg
     3481 ccaagggggc gcctcgcccg cggggatctg cagtcccccc gcgagcgagg tggactggtg
     3541 caccggcggg cgggaaggcg cgctcttcac cgacgcctgg cgagccttcg agtcctggga
     3601 ctgagggcgg ggtccgggag ccggcggcgg gttgtggggc ccgccgccgg cacattcccg
     3661 gctaccagcg cgcgcgggcg gtgcagagct gcccgccgga gagcgcccgc gcccgggcgg
     3721 tgatcgtctc gcgcccggcg cggtcggcga cgaggcggcg cacctcgaac gagccgctcg
     3781 gcggctgcgt cgtgcggatg ccgcggaaga cccggacccc gttgcgcttc agggtcacgc
     3841 gccagcggcg gccgttgacg ttctggtcga cctcgaactc gacctcgatc cggccgttct
     3901 cctcgctcag cttgagcttc gaggtcgccg cgcccgtgca cgtgccgcgg acgcggacct
     3961 cgcggtctcc tccgccgctc gccggcgccg cgaaggccag ggacgtcgcg gccgcgatcc
     4021 cggccgcgag ggcgacgatc aggtgcctcg acttcatctc gctcctcctc ctcgggtgac
     4081 gggctcaccg taggagggag cgggcgaagc gcgggccacg cctcggttca ggttcggcca
     4141 ggcgcgggtt aaggctcgga ggttcccgct ccgcgagcgg tgactatcat cagaccgagg
     4201 acggctgatg gaggagatgg tcatctacgg ggtgagcttc gacatggtcg gcaagcagcc
     4261 gatcgtgctc ctgaagaccg ccgacgggaa caagttcctc cccatctgga tcgggcatcc
     4321 cgaggcggcc gcgatcctca tgaagctcca gggcgcgtcg acgccgcggc cgatgacgca
     4381 cgacctcttc acggacatgc tcgcgcagct cgacgcgcgg gtcgtccgga tcgcggtcac
     4441 cgagcttcgc gacaacacgt tctacgcgct cgtcaccctg gccgtgaacg ggagcgagat
     4501 cgagatcgac tcgcgcccct ccgacgcgat cgccctcgcc gtgcgcgcgg acgcgccgat
     4561 cttcgccgcc gagagcgtga tcgaggagtc cgcgatcgag ttcgagggcg aggaggtgaa
     4621 cgaggagcag gtcgtcgagg agttcaagca gttcctcgac gaggtgaagc ccgaggactt
     4681 ctccgccgag gcggacccgg actgacggct agctcacctc gcgcttcgtg agcgcgagga
     4741 tctgcgcgaa gagctcgcgg aggccgtcgt ccgagagcgg gccgccgttc gcccgctcga
     4801 ggtagcggat catccagtcc tcccggtccg ggtcgacgaa ctcgaacccg cgggaggcct
     4861 tgtactcctt caggcgcgcg acgagcttca ggcgcttgtt gatcgcgtcg acgatcgcgc
     4921 ggtcgttgtc cgagatctgc tcccggagct ggcggacgag cgggtccgtg ctggcgtcgt
     4981 cgggcggcac ggccgcgagc ctacgcgaag acgtggatca cggtgccgat cttcccccag
     5041 cggcggtaga agcccggcgc gaaccacatc ggcgtccgga cgcagccgtg gctcgccggg
     5101 tacgtcggca cggacgggta gccgtggatc gcgaagccgc ggaggaagaa gagcgagtag
     5161 tacatgccgc tcgggagggt cccgggcgtg agcaggtaga cgcggaactt gccgacgggc
     5221 gtgttcccgg tcgcgccggt cgagacgtgg acgaccttca cgaccttccc gtcgcggacc
     5281 tcgtacatga cctgggtcgt cttcgacacc tcgatgtggt cgccgcgcgg gacgcgggcg
     5341 cgcgggacgc cggacgtgcg cagcagccgc cattcctgcg cgccgacgcg gccggtgcgc
     5401 gggaggccgt gcaccttctg gagcgcgaag acggcctcga cggtgtcgta gccgtacgtc
     5461 gagttcacgg tcgcgagggc gtagcggagc cggttcagcc gccgctcgag cgcgaggacg
     5521 gcctgcccgc gcgcgccgag cgcgagcggc ggcgccgtca ggacgagcgt gttcgcgcgc
     5581 cgcgccgcca ggtagcccgg cttcggcgcg accctgagcg accagcggaa ccggccgggc
     5641 acgtccgtcg ggagcgtcac ctggaagcgc ccgacgccgc cgacgcgcac gcgccgcgcc
     5701 ttccctgcga agcggagcgt gagcctgccc gcggccgccg gctgcattcg cccgcgcagg
     5761 acgacgcgcg ccccgaccgt ccgcttcccg accacctcgg cggtgagccg cggcttgagc
     5821 gggacgacga gctgcggcga gtcgaccgtc gtcgtggcgt cgagctgggc cctcgcgagg
     5881 tacgggccgg gccgggtgag ggcggcgggg acgctccacg agccgtctgc cgcgacggtc
     5941 gtctgcccga cgagcgaaac gcccggagcg agcgagaaca cggcgaccgc gacgccctcc
     6001 gtcgccggcg tgagcgtccc ggagaacgtc accgtcccgc cgaacttcgt cgaggccggc
     6061 gcggcgtcga gggtcagcgt ctgcgcgtgc gcagcggccg gaagcgcgag ggcgaggaat
     6121 ccgatcgcga cgacgcgacg cacgccgtca ttgtgcgcag accggtcggc cggagcaaga
     6181 ggctcacatg acgcagccga gcgtcgcgcg gcactccgcg tcggagaggc acaggagccc
     6241 gatcgagagc acggctccgt tgtagagcgc gtgcccgatc acgcacggcc agacgctcgt
     6301 cgagcggagc cggacccacg ccagcccgat tccgaagacc gcgagcggga ggaggccgat
     6361 caggagcccg tgcgcgagcc cgaacgcgac gccggtgacg accatcgcgg tgacggcgcc
     6421 gatgctctgg agcagcccga ccccggcccc gcggaagaag agctcctcga cgagcggcgc
     6481 ggcgacgacg atcacgaccg cgttcgcggc gatcgctccc gcccgctcgg gccgccagtc
     6541 ctccgggagg atgccctgct ccgagcccgc gtcgagcccg aggagcgcgg cgatgaggta
     6601 ggagagcgcc gcgacgccga tcccgatccc gatcgcggcc ccgacccagc gccacgcgaa
     6661 ggagcgcagc ccgagcacga cggacgggcg ccccgcgatc cccgcgatcg cgaccgtgag
     6721 ccccaggagg acgaggtaga cgaacgcgtt gccgatcgcc gtgccgtact cgtagaggcc
     6781 gtcgcgcccg acctcgtcct cgtccacgag gagcatgatc atgaatgcga gcgcgatctg
     6841 gagcgcggcg agcacgcccc aggcgacgat cgcgaggccg cgcctagagt cgctcgagcg
     6901 ctcgggcgaa gtcggcgacg acgtctcgtc catcctcaca gcctacggag acacggatcg
     6961 tcccctcgga gatgcccgcg gcggcgagct gctcggcgtc gaactggcgg tggctcgtcg
     7021 acgcggggtg gtggacgacc gtgtccacgc cgccgaggct cgtcgcgcgg gctgcgagct
     7081 cgagcgcgtc catgagccgc tcgcccgcct cgcggccgcc cgggacgtcg aacgcgagca
     7141 tgccgccgga gtcggcgagc tgccggagcg cgagctcgtg ctgcggggag ctcgcgaggc
     7201 cgacgtagta gacgcgctcg accttcggat gcccctcgag gaagcgggcg agctcgagcg
     7261 cgttcgccga ctggcgctcg acgcgcagcg cgagcgtctt caggccgcgg cgcaggaggt
     7321 acgcggggaa cgcgtcgagg atcgcgcccg tgtcgatctg gaacgtgcgc acgcgcgcga
     7381 cgagctccgt gcttccggcc acgaccccgg cggtcacgtc gtggtggccg ttcaggtact
     7441 tcgtggccga ctcgcagacg aggtcggcgc cgagctcgag cggccggcag agcacgggcg
     7501 aggcgaacgt gttgtcgacg atcaggagcc cgtcgccctt cgtcgccgcg agcgcctcga
     7561 ggtcggcgag cgggaatcca gggttggcga gcgtctcgac gtagcgcacg gcggccggcc
     7621 gctcccacgc ctcgaggtcg gtcacgtcga ggtagtggac gtccacgttg aggcgccgca
     7681 cctgcgactc gacgaacgcg tacgtcgacc cgtagagctg ccgggcgcag agaagcgtgt
     7741 cgcccggcga gagcgaggtg aggagcgcgg ccgcgatcgc ggccatgccg gagccgaacg
     7801 cctgggccgc ctccgccccc tcgagctcgg cgaccgcgcc gttcagctcc tcgacggtcg
     7861 ggttgcgcag gcgcgagtac gtgaagccgt actcctcctc gctcatcacg cgccccatct
     7921 cggcggccgt ctcgaaggag aacgtcgtcg aggtgacgat cggcggggcg agcggctcac
     7981 ccggtgcgtg cgggcggccg cgcccgtgga tcgcacgggt cgagatcccg ggccgctcgt
     8041 ccgtcatccg ctcgccttcg ccatgcgcgc gggaagcgta ctgagcggcg gcggcgcgaa
     8101 cgcgagacca gccgcctcgg ccccgagccg gtcggcctcg tcgtccccca cgtgcagcgt
     8161 ctcgcccggc tcggcgccca gccgctcgag cgcgaggagg aacggccgcg ggtcgggctt
     8221 cggggcgccc gcctcggcgg agctcacgat cgcgtcgagc cgcgcggcga gcccggccgc
     8281 cgcgagcgcg tccgcgagcg agacgtccca gttcgagacg cacgcgaggc ggaggccgcg
     8341 ggcccgcagc gcgtcgagcg cctcgaccgt cccggggagc ggccggaagg cgagcgcccg
     8401 gacgaacgcg ggcgcgaagc cggccgggtc gaggtcggcc cctgcctcgg cgaggaagac
     8461 ggccgcgcag tcccggcgga gcgcggcgag gctcgccgcg tcgcggccct cgtgcgcgcg
     8521 ggggacgtag tgcgcgacct cggccgcgaa ggcccgcgcg acgacctcgt cgtcccgctc
     8581 gacgccggcg gccgcgagcg ccgcgcggag cggctcgacc gggtcgacga gctcgacgag
     8641 ggtcccgaag gcgtcgatcg tgaccgcccg aggcggctca gaacgcatcc gggtggagcg
     8701 cgcgggcgat ggtgtcgagc gcctcgccga cgcggggccc ggggcggact gcgaggtctc
     8761 ccgggatccg gacgacggtc acgtcgacgt tcggccggcg cgggcacacg aggcgcgtgt
     8821 ccggcagcgc ggccaccggc tcccggatac gaaggacgac ctccggccgc agcgcgagca
     8881 cctggcacgg ctcggcggga gcgccgtcgt tcccggtgcc gaccggctgg ccgcctgccc
     8941 ggcgaacgag gtcgacgacg agcgagtccg gggacggggc gatcagcagc ccgaggtcga
     9001 cgaagacgcg caccggcgcc cggccgcgga cggcctcctc gaccgccgcg agctcctcgc
     9061 ggagcgaggc ggcgaacacc cgcgcgcggg ccggctcccc gaggaggaac ccaaggtcgt
     9121 gcacggcgcg gacgacgtcg cggatcgacc ggtccggctg gacgtagacg ggagcccccg
     9181 tgcgccgggc gatcgtctcg aggtcgccgg cgggggcccc ggagcccgcg agcacgagat
     9241 ccggctcgag ccgggcgacg gcgccgatgt cgacgaaccc gctcgggcgc gtcacccggg
     9301 gcgcgccgag cgccccgcgg acggtcaccc cggccggaac gccgaccaca cgctccccgg
     9361 cgccgagctc gccggccagt tccgcgacgg agggcgcgag cgcgacgatc cgctccggcc
     9421 ggccctcgag ctcgatctcc tcgccgcggg cgtccccgac gagcaccggg tacgcgtcga
     9481 cgtcctcccc gagcggctcg tcgcgctcgc cgcagccggt cgccgcgacg aggacgagga
     9541 ggaggccgag gagcgcgggc gcacgggatc gcatcccgag gagtgtgccc agtccgcgcg
     9601 gggcaggcaa aggcgcggca ccggagaaca ggacgctcgt ggactcgcag gactggaagc
     9661 gcgtcgcggt cgccggcgcg ctcatcctca tcgcgatcgt ggtcgcgcgg ctcgccgacc
     9721 gcgcgatcac gcggaggatc gagctgacgc ccgagcagct gacgcgctac cgcgtcctgc
     9781 gccgcagcgt gaccgcgacg atcgtcgcga tcggcgtcct gagcgccctg ctcgtcatcc
     9841 cggagatccg cgcggcggcg ggcggcgtgc tcgcgtcgtc cgcgctcctc ggcctcgtga
     9901 tcgggctcgc cgcccggacg acgatcgcga acttcgtcgc cggagtcctg ctcgccttca
     9961 cgcagccgct gcggatcggc gacgaggtcg aggtcgcggg ggcgcagggc acggtcgagg
    10021 agatctcgct cacgtacacg acgatccgca cgccgggcgg agcgcggttc ttcgtcccga
    10081 acgagaagct cgcgtccgag acgatccgga acgcgaccgc agggacggcg gagcatctcg
    10141 cgcgggtgac cgtgccggtt ccgctagcct cggatctcga ccgggccctc gccctcgtgg
    10201 aagaggaggc ccggaccgcg agtcacgtcg aggagaagcg cgccatcgca acgatctctc
    10261 agttcgagcc cgccggccag gcgatcctga ccgtggaggc gtgggctccg cgcgggcagg
    10321 tcgccgcggt cgagacggag atccgccagg ccgtccaccg ccgcctccgc gcggaggggg
    10381 tcttctagtg ccgccggccg ggcggaagcg cccgaacggg aacggcaacg gcgccgcacg
    10441 ggcgcggaac ggcaacggtg gcacgcggcc gcccacccgt ggtcgccgcg agcgtgcgcg
    10501 ccgccgccgc gcggcccgcg gcaagcgccg cgcgatcgtc ttcctcgtcc tggtcggcgc
    10561 cgtctcggcg ctcgcggcga tcgcggccgg cgtcttcacc ggcgcctccg cggcgctcgc
    10621 gtgcgacctc tcctcgctcc gcccggtcgc gatcgggcag aactccttca tctacgccgc
    10681 ggacggctcg ctcctcggct cgatcccggc cgagcggaac cgcacgccgc gcgcgctcga
    10741 cgagatctcg ccgctcgtcc aggaggccgc ggtcgcgatc gaggaccgcc gcttctacga
    10801 ccacatgggc gtggacgtcg aggggatcgt gcgcgcggcc gtcaagaacc tcgaggcagg
    10861 cgagatcgtg gagggcggct cgacgatcac gcagcagctc gtccgcaacc tctacatcgg
    10921 caacgagaag agcttcaacc gcaaggcgaa ggaggcgtgc ctcgcgctca agctcgacgc
    10981 cgagtggacg aaggagcgga tcctcgagac ctacctgaac caggtctact tcgggaacca
    11041 cgcgtacggg atcgaggcgg ccgcgcagac gtacttctcg aagccggcgg aggagctcgg
    11101 gctcgcgcag gccgccctcc tcgcgggcct
//
LOCUS       DASTFC010000084        14155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_841951,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000084 DASTFC010000000
VERSION     DASTFC010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14155
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841951"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFO88_02500"
     CDS             <1..415
                     /locus_tag="VFO88_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HET9544423.1"
                     /translation="FVFCRPEASGEEHEGLLAAEEALVQELGLPYRVVNIAAGDLGAS
                     AAKKYDIEAWFPSQGRYREVTSTSNTTDYQARRLEVRFRGERGVEPVHTLNGTAVTAR
                     HMLAVLENFQDEGGAVTVPGALVPFGAPARIEPGE"
     gene            604..693
                     /locus_tag="VFO88_02505"
     tRNA            604..693
                     /locus_tag="VFO88_02505"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:638..640,aa:Ser,seq:tga)
     gene            complement(708..2522)
                     /locus_tag="VFO88_02510"
     CDS             complement(708..2522)
                     /locus_tag="VFO88_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET9544424.1"
                     /translation="MRRAVSGLLAGAVLAAALALAGPAAAGYKAKVAGGTLTLTGNAK
                     GDRLALRLKKNAAGTLQVDVGANGSVEFSFRRSRFTAIVVNAGGSHDTVTIDERRGVF
                     TTTEATTLNGGAGNDRLNGGTGGETLDGGPGKDVLNGKGGADTARGGPGADTVSLGGG
                     DDSAVSLLGGGADVVTGGPGTDLFRMAGTSGGDALAARAEGAAARVTRNVDNASVRVA
                     TVERLELDAGVGADDVTVSDLTDSGVTHVEVELGTGGAGDGADDRVFVEGGSMPDVLT
                     AAAVMSMVQVTGGAAQVDVLGAEPALDRLNLAGLGGNDTLSGSALASLVMLTLDGGTG
                     NDVLNGGNGADLLLGGSDNDTIDGNQGADVALAGEGNDSFVWDPGDGSDVVEGQEGDD
                     TLVFNGSAGAEIFTAAANGGRLLFTRNVGNIVMDTDGVEALVVNALGGTDSITVNSLG
                     ATDVTNVTTDLGVGGASDGAADTVTMNTSAGLDAVTVSGSSGSFVLSWSGFVLSGLGA
                     AAATDSLTVNLLGGDDVFSAGGLAATSVGLTANGGTGNDVLVGSAGNDTLNGDANDDF
                     VDGNDGADTIDCGANTDVADGGAGIDSAANCETTFNVP"
     gene            complement(2790..4700)
                     /locus_tag="VFO88_02515"
     CDS             complement(2790..4700)
                     /locus_tag="VFO88_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9544425.1"
                     /translation="MRRETNRNDVRSSALWGKRSGSREGRKLGRRGFAAALVAGLVML
                     AGPLAGVADASGKDYEAYVTPTLLERAEANGDRLFEVIVQSDGTMPSAALAGMIVREG
                     GSTATAQARVRSRIKDEFDTVSAVAVTLSGKQILRLADRRGVYAITPDAPVELSNYSN
                     KQLWPLATGVAKVWPSMANGTAGTMPAIAVVDSGIDSSRNDFGGRVIVEKTMTSLAGN
                     SPGDGRGHGTFVAGIAAGSAPGYAGAAPSAPIVSIDVMDDKGMAMTSDVIAAADWIHA
                     NRRTYDIRVANFSLHSSVPNSFMYDPLSKAVERLWFAGVVVVAASGNYGVANAPSGVP
                     FAPGNDPFIITVGALDLGEKLNVKDDGSAPWSAYGYTLDGFLKPEVGAPGRYMVGPVP
                     ATSTLALERPEQLRAAGYIELSGTSFAAPLVSGMAAYILARNPTWTPDQVKGALMLTA
                     THLPSADFGSVGVGFVSADKAAAVKDPPNPNLALNAFLVSDPAGSGDLVFDSASWAHV
                     AKEDASWASASWANASWANASWANASWANASWASASWASASWASASWANASWAAASWA
                     SASWASASWANTAEAAASWANASWASVSYEDNAAGEDGIGGLVLSPEEEAFLLGLLED
                     APAEEPVVEILP"
     gene            4856..5791
                     /locus_tag="VFO88_02520"
     CDS             4856..5791
                     /locus_tag="VFO88_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9544426.1"
                     /translation="MPPFVRVGTCSWADESLSKWWYPRGVNGAEERLRYYAERFDTVE
                     LDATYYRLPEAEQVAKWVERTPPGFVFHVKAFGLMTRHPVKLEALPPELREGMPVDER
                     GRVDRPPRELRARVFERFHEALEPFRAAGKLGGILFQLPSYVVRKPVSLDYLAWAKEE
                     LRGDEMLVEFRHRSWLDDEGRAETLAFLEELGASHVVVDAPKTEAKNLVPTVLALTSP
                     TLYVRFHGRNAATWNKRGGSAAERFDYLYSDEELEEWAEAIGPLAEEAENAYAMFNNN
                     GRSPSPPGLGERDWFAQAPVNALRFKELLGQEVPA"
     gene            5788..6453
                     /locus_tag="VFO88_02525"
     CDS             5788..6453
                     /locus_tag="VFO88_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544427.1"
                     /translation="MSPLETLAYRRRDALGDPEGALVVCHGRGADEHDLAPLLDALDP
                     ERRLAGFLPRGPLSLPPGGAHWYVVHEVGFPDPATFLPTFETLSGWVDAALADAGVPI
                     ERTVFAGFSQGTVMSYSLAFAASRPRPAGVLAFSGFVPRVDGFELDLEGRAGLPVSIA
                     HGTHDPIITVGFGRDARERLQAAGVDVAYVEEPMAHSIGPAGLAQAREVLERALGLDE
                     QPD"
     gene            complement(6450..7196)
                     /locus_tag="VFO88_02530"
     CDS             complement(6450..7196)
                     /locus_tag="VFO88_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HET9544428.1"
                     /translation="MERDWAQRGVAEAIGAFALVFIGAGSIAVAGPADGGLVGVALAH
                     GLVIAVMVSAVGHISGGHFNPAVTLGFLVTKRMAPSLAGVYWAAQMLGGLLGAILLTL
                     LLPNELTDPVALGTPALGLGIGAGAGVVIEAILTFFLVWVIFATAADPRGTFTAIAGL
                     AIGLTITLDILMGGPLTGAAMNPARAFGPQLVAGEWGDFWVWYLGPLVGGAAAALAYE
                     ALYLRPARPEPVGTPGSGLDEPRPGDAASR"
     gene            complement(7399..8889)
                     /locus_tag="VFO88_02535"
     CDS             complement(7399..8889)
                     /locus_tag="VFO88_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET9544429.1"
                     /translation="MDVGSALDLVGGVLKYLGGAFLVPAAVAVGYGEPWWPFVVAGAV
                     TAGVGWSLDRLVTGTREQVGPREGFLVVALIWLLVPAFGALPYLLGDVEQLSSPVNAY
                     FEAVSGFTATGATVLTDIEALDRSMLVWRQLTHWLGGMGIIVLAVAVLPRLRVGGRQL
                     LSSELAGPTELERLTATIRDTARRLWALYVGLTATCILGLALLGWTGIDPEMDLFQAV
                     AHAFSVLALGGFSTQNESAAAFVPATQWLLVVFMVLAGINFLRLYRILVQRHVRVAAR
                     DEELRLYAAFLLAASLLLFVELLADGIAVGEGAVRDSVFQAVSIMTTTGFATADYTGW
                     TALATLTLLLLMFVGASAGSTGGSLKVVRHLLMVKLIRRELRQAVHREAVIPVRLSGA
                     VVDERALRSAFAFVVLYLGIFALGTLGLLIDARRGEVEVATFEAIGAAAACLGNVGPA
                     FGFAGPFGSYEPFGDLSTGILSMLMWLGRLEIVPVVVLLMRSYWRA"
     gene            complement(8981..10411)
                     /locus_tag="VFO88_02540"
     CDS             complement(8981..10411)
                     /locus_tag="VFO88_02540"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HET9544430.1"
                     /translation="MGLVLLAGVATAFTAARASVPVLVAFLGVGMLLGSEGLGGIEFD
                     DPDLARTVGIVGLVAILFEGGLATRWRDIRPIVVPAALLSTVGVAVTALAAGLAAYLL
                     FDDLSLSVALLLGAVVGSTDAAAVFATLRFTSLRRRLAALLEAESGANDPMAVALTLG
                     LVAWVTTDGYGGRDLALLLVRQLGLGLVLGLALGLLAAWVFPRLPAELAPFAPVLSLA
                     TAAVSFGVVDVAGGSGFLAVYIVGLFIGNVPGPFTRAIVAFHHGLAFLAQIVLFVVLG
                     LLVFPSNLAEVILPGLALAAVLLVVARPLAVLASLPFPVPGLGVRERVFVGWAGLRGA
                     VPIVLATFALSADVAESDLIFDAVFFVVLVSAVTQGPTLEPLARRLGLATEARPLHVE
                     PLEVGAIRSLGADVLEFPVDERSRVVGKRLRELGLPADAVVVVIVRDLAAVPPRGSTE
                     ILAGDRVYVLVRGAARPAVEDVFTAV"
     gene            10522..11709
                     /locus_tag="VFO88_02545"
     CDS             10522..11709
                     /locus_tag="VFO88_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9544431.1"
                     /translation="MTLTQLAVTVALLGVLGLLASRFGLSAIPAYLLAGLLLGPNEPE
                     ALTVIRPSEVTEFVADLGVIFLLFFLGLEFSVGRLLRSGRHFGAGGSIDLVVNGGIGL
                     ALGLAAFGPGIAAVVFAAAVYISSSAVTVKGLIDFRRLADEETDLVLAILIFEDLVIA
                     FVLGFAATGGGGFGPTFVLVAKALAFVGLTLVASRYASRAIAMTLERMPREFFLLFSF
                     ALLIGMSAVAERLGLSEAIGALMAGVVLSETAVRAELEERFFSFRDLFAALFFFVFGL
                     TIDLDALGSVGWLVAVAVVLTVAGKLFAGYAAGQVGGFTRGQSLNTGAALVAHGEFTV
                     IIAQLASRNPQISPTTRSDLVAFAGLYVLATATIGVVLMKESKRIGRRLFRRPTLTQE
                     ALE"
     gene            11706..12242
                     /locus_tag="VFO88_02550"
     CDS             11706..12242
                     /locus_tag="VFO88_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HET9544432.1"
                     /translation="MNQVTQTRLPGVGTKFSIPTSHGGRLAVIQHNDGMREIYFFQRR
                     DDDEPCAVIQLEDDEARQLGAVIGGAYERPRIVEELEMAFGELAIEWVPVPQGSPAIG
                     HSLAECAFRARTGITIIAILREPEPVTGAQPGDVIKEGDTLVTVGKLGQYTAFRKLLA
                     EGPFPAPNQEPTHIPKRL"
     gene            complement(12387..12884)
                     /locus_tag="VFO88_02555"
     CDS             complement(12387..12884)
                     /locus_tag="VFO88_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544433.1"
                     /translation="MLLVLLGVAVLLFSVAACGGDDGDVAAEGEVVEVELGESGSTYY
                     VEPAQTTVEAGTVTFAVTNMGELYHEFIVYSNLDGVEPGDLPITEEDKADLVEENIVG
                     EAAYATPPIVPSDSEPGAADHHIRGGGWGAELTVDLEAGEYILLCDITGHYGTYKQYA
                     AFTAE"
     gene            complement(13110..>14155)
                     /locus_tag="VFO88_02560"
     CDS             complement(13110..>14155)
                     /locus_tag="VFO88_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HET9544434.1"
                     /translation="TNVEYLEIWRERELEIDFMVCSELEAAHAIARLVGVPAARQTDV
                     FADGLVQMIELDVEVEPGGDDGEVIGRPLREARIPDESKVAAIIRGDSVLVPRGDESV
                     LRGDRVVIIGSPAAAREWSRLMLRGRTAVVDDVVVFGGGQTGFEVARTLTDQGIGVRL
                     IEADGERAREVAEALPGVAVFHATGVDPEFLERERIGRSQVAVFAMREDAKNLYAATL
                     AKLHAVAFTVAIVHEPGAELVFERAGVDAAVNPRSVTAEEMVRFAHDPRIRQLAMLEG
                     DRFEVLDITVREESELCGIPFRELPMTGSLIGAIVRDGEAIFPHGEDVLLPGDRAIIF
                     TESSRVANVEKVL"
BASE COUNT         2022 a         5217 c         5051 g         1865 t
ORIGIN      
        1 gttcgtcttc tgccggccgg aggcgtcggg cgaggagcac gagggcctgc tcgcggcgga
       61 ggaggcgctc gtgcaggagc tcggcctgcc gtatcgggtc gtgaacatcg cggcgggcga
      121 cctcggagcg tcggcggcga agaagtacga catcgaggcg tggttcccga gccagggccg
      181 ctaccgcgag gtcacgtcca cctcgaacac gaccgactac caggcgcggc ggctcgaggt
      241 gcgcttccgc ggcgagcgcg gcgtcgagcc ggtgcacacg ctgaacggga cggccgtcac
      301 ggcgcgccac atgctcgccg tgctcgagaa cttccaggac gagggcggcg ccgtgaccgt
      361 ccccggggcg ctcgtgccct tcggggcgcc ggcccgcatc gagccgggcg aataggccgt
      421 cccatgctcg gcgggcccgc gccgcgggcc tcctccccct aggggatcgc gagcatcccg
      481 ggaggaacgc tagcggcgcc cggcgcacgc gtctgtgccg aacgcgtgac ggattcgtgc
      541 ccccgcccgc ggcgcggccg cgtgcgcttg tgagagctcc gcgccccgct agactcgccc
      601 gcgggcgggg tgcccgagcg gtcgaaaggg gcggtcttga aaaccgctag gcgagtgacc
      661 ctcgcctcgt gggttcgaat cccacccccg ccgttcgccc gcgccggcta ggggacgttg
      721 aaggtcgtct cgcagttggc ggccgagtcg atcccggcgc cgccgtcggc cacgtcggtg
      781 ttcgcgccgc agtcgatcgt gtcggcgccg tcgttgccgt cgacgaagtc gtcgttcgcg
      841 tcgccgttca gcgtgtcgtt gcccgcgctt ccgacgagca cgtcgttgcc cgtgccgccg
      901 ttcgcggtca gcccgacgct cgtcgcggcg agccccccgg cgctgaagac gtcgtcgccg
      961 ccgagcaggt tcacggtcag gctgtcggtg gcggccgccg ctccgaggcc gctcaggacg
     1021 aacccgctcc agctcaggac gaacgagccg gagcttccgc tcaccgtcac cgcgtccagg
     1081 cctgcactgg tgttcatcgt caccgtgtcg gccgctccgt cgctcgcccc gccgacgccg
     1141 agatcggtgg tcacgttcgt gacgtccgtc gcgcccaggc tgttgaccgt gatcgagtcg
     1201 gtgccgccga gcgcgttcac gacgagcgcc tcgacgccgt ccgtgtccat gacgatgttg
     1261 ccgacgttcc gcgtgaacag gagacgtccg ccgttcgccg ccgcggtgaa gatctccgcg
     1321 cccgccgagc cgttgaaaac gagggtgtcg tcgccctcct ggccctcgac gacgtcgctg
     1381 ccgtcaccgg gatcccagac gaacgagtcg tttccctcgc cggcgagggc gacatcggcg
     1441 ccctggttgc cgtcgatcgt gtcgttgtcg gatccgccta ggaggaggtc ggcgccgttg
     1501 ccgccgttca ggacgtcgtt ccccgtgccg ccgtccagcg tcagcatgac gagcgaggcg
     1561 agcgcgctcc cgctcagcgt gtcgttcccg ccgaggccgg cgaggttcag ccggtccaag
     1621 gccggctcgg cgccgaggac gtcgacctgc gcggcgcccc cggtgacctg caccatcgac
     1681 atgacggcgg cggcggtgag gacgtccggc atgctcccgc cctcgacgaa cacgcgatcg
     1741 tccgccccgt cgccggcgcc gcccgtcccg agctcgacct ccacgtgcgt gacgccgctg
     1801 tccgtcaggt cggagacggt cacgtcgtcg gcgccgaccc ccgcgtcgag ctcgagccgc
     1861 tccaccgtcg ccacgcggac gctcgcgttg tcgacgttcc gcgtgacccg cgccgcggcg
     1921 ccctcggccc gcgcggcgag cgcgtcgccg ccggacgtcc ccgccatccg gaagagatcg
     1981 gtgccggggc cgccggtcac gacgtccgcc ccgccgccga ggagcgagac ggcgctgtcg
     2041 tcgccgccgc cgagcgagac ggtgtccgcg cccggtccgc cgcgcgccgt gtcggcgccg
     2101 cccttcccgt tcaggacgtc ctttcccggc ccgccgtcga gcgtctcgcc gccggttccg
     2161 ccgttcaggc ggtcgttccc ggccccgccg ttcagcgtcg tcgcctcggt cgtcgtgaag
     2221 acgccgcgcc gctcgtcgat cgtgaccgtg tcgtggctcc cgcctgcgtt cacgacgatc
     2281 gccgtgaagc gcgagcgccg gaaggagaac tcgaccgacc cgttcgcacc gacgtccacc
     2341 tggagcgtgc cggcggcgtt cttcttcagc ctgagcgcga gcctgtcgcc cttcgcgttg
     2401 ccggtgagcg tgagcgtccc gccggcgacc ttcgccttgt agccggccgc ggcgggcccg
     2461 gcgagggcga gcgcggccgc gagaacggcg ccggcgagaa ggcccgagac ggctcgtcgc
     2521 atgtgccgga cgatacgccg tcgcgtggcg aatctccaga ctggcgaacc gcagcgccgt
     2581 gcgacggccg gaacggcgag cgcccgcccg gcccgtgggg gccgaacggg cgcgcctccc
     2641 cgtcctacgc cttcggccga ggctcagctg ccgcgctcac cggagcgctt cccccacagg
     2701 gcgctgagcc ttccgctgtc gctcttcgac attcgtccgt ccttcctcgc cgcccgtgcg
     2761 gggcggccgc agtaccggga tcgacttcgt tacgggagga tctccacgac cggttcctcc
     2821 gcgggtgcgt cctcgaggag gccgagcagg aacgcctcct cctcgggcga gagcacgagc
     2881 ccgccgatgc cgtcctcgcc ggcagcgttg tcctcatagc tcacgctcgc ccaggacgcg
     2941 ttcgcccagg acgccgccgc ctcggccgtg ttggcccacg aggccgacgc ccaggaggcc
     3001 gacgcccacg aggccgccgc ccaggaggcg ttcgcccagg acgcactcgc ccaggacgcg
     3061 ctggcccagg aggcgctcgc ccacgacgcg ttcgcccagg aggcgttggc ccaggacgcg
     3121 ttcgcccacg aggcgttcgc ccaggaggcc gacgcccacg acgcgtcctc cttcgcgacg
     3181 tgcgcccagc tcgccgagtc gaagacgagg tcgcccgagc cggccgggtc cgagacgagg
     3241 aacgcgttca gcgcgaggtt cgggttcggc ggatccttga ccgccgccgc cttgtccgcc
     3301 gagacgaagc cgacgcccac cgacccgaag tcggccgagg gcagatgcgt cgccgtcagc
     3361 atgagggcgc ccttgacctg atccggcgtc cacgtcgggt tcctggccag gatgtacgcg
     3421 gccatccccg agacgagcgg ggccgcgaac gacgtgcccg agagctcgat gtagccggcg
     3481 gcgcggagct gctcgggccg ctcgagcgcg agcgtcgagg tcgccggcac gggcccgacc
     3541 atgtagcggc cgggagcccc gacctcgggc ttcaggaagc cgtcgagcgt gtagccgtac
     3601 gccgaccacg gagccgagcc gtcatccttc acgttcagct tctcgccgag gtcgagggcg
     3661 ccgaccgtga tgatgaacgg gtcgttgccc ggggcgaagg gaacgcccga gggcgcattc
     3721 gcgacgccgt agttgcccga ggccgcgacg acgacgacgc cggcgaacca gagccgctcg
     3781 accgccttcg agagcgggtc gtacatgaag ctgttgggca ccgacgagtg gagcgagaag
     3841 ttcgcgaccc ggatgtcgta cgtgcgccgg ttcgcatgga tccagtcggc ggccgcgatg
     3901 acgtcgctcg tcatcgccat gcccttgtcg tccatcacgt cgatcgagac gatcggggcg
     3961 gacggcgccg cgccggcgta gccgggagcg ctccccgccg cgatgcccgc gacgaacgtg
     4021 ccgtggccgc ggccgtcgcc gggcgagttc cccgcgagcg acgtcatcgt cttctcgacg
     4081 atcacgcgcc cgccgaagtc gttccggctc gagtcgatgc ccgagtcgac gacggcgatc
     4141 gccggcatcg tgcccgcggt cccgttcgcc atcgacggcc agaccttcgc cacgccggtg
     4201 gcgagcggcc agagctgctt gttcgagtag ttcgagagct cgaccggggc gtcgggcgtg
     4261 atcgcgtaga cgccgcgccg gtcggcgagc cgcaggatct gcttcccgct cagcgtgacc
     4321 gcgaccgcgg acaccgtgtc gaactcgtcc ttgatccgcg agcgcacccg cgcctgggcc
     4381 gtcgcggtgc ttccgccctc gcggacgatc atgcccgcga gcgccgcgct cggcatcgtg
     4441 ccgtcgctct gcacgatgac ctcgaagagg cggtcgccgt tcgcctccgc acgttcgagg
     4501 agcgtcgggg tgacgtacgc ctcgtaatcc ttgcccgacg cgtcggcgac gccggcgagg
     4561 gggcccgcca gcatgacgag gcccgcgacg agcgccgccg cgaacccgcg ccgaccgagc
     4621 ttccgaccct cacgggagcc ggagcgcttg ccccagaggg cgctcgagcg cacgtcgttc
     4681 cggttggtct ctctccgcat ctgtcccgtc cttctctccg ccgtcccggg cggttccgca
     4741 ggtgaaaagg gaatgcacgc ggaacgctag gtcgcgagac ctttagccac ttcccccgaa
     4801 cggggcacgg ccgggaagat cactcggacg agggatcgcc cgctagcatc cgcgcgtgcc
     4861 ccctttcgtt cgcgtcggca cctgctcctg ggcggacgag tcgctctcga agtggtggta
     4921 cccgcgcggc gtgaacggcg cggaggagcg gctccgctac tacgcggagc gcttcgacac
     4981 ggtcgaactc gacgcgacgt actaccggct gccggaggcc gagcaggtcg cgaagtgggt
     5041 cgagcggacg ccgcccggct tcgtcttcca cgtgaaggcg ttcgggctca tgacacgcca
     5101 ccctgtgaag ctcgaggcgc tgccgccgga gctccgcgag gggatgccgg tggacgagcg
     5161 ggggcgcgta gaccggccgc cgcgcgagct ccgggcccgg gtgttcgagc ggttccacga
     5221 ggcgctcgag ccgttccgcg cggccgggaa gctcggcggg atcctgttcc agctcccctc
     5281 gtacgtcgtg cgcaagcccg tgtcgctcga ctatctcgcc tgggcgaagg aggagctgcg
     5341 cggggacgag atgctcgtcg agttccggca ccggagctgg ctcgacgacg aggggcgggc
     5401 cgagacgctc gccttcctcg aggagctcgg cgcgtcgcac gtcgtcgtcg acgcgccgaa
     5461 gacggaggcg aaaaacctcg tgccgaccgt gctcgcgctc acctcgccga ccctctacgt
     5521 ccgcttccac gggcgcaacg cggcgacctg gaacaagcgc ggcgggagcg cggccgagcg
     5581 gttcgactac ctctactcgg acgaggagct ggaggagtgg gccgaggcga tcgggccgct
     5641 cgccgaggag gcggagaacg cgtacgcgat gttcaacaac aacgggcgct cgccgtcgcc
     5701 gcccgggctc ggcgagcggg actggttcgc gcaggcgccg gtcaacgcgc tccgcttcaa
     5761 ggagctgctc gggcaggagg tgccggcgtg agcccgctcg agacgctcgc gtaccggcgc
     5821 cgggacgcgc tcggcgaccc cgagggcgcg ctcgtcgtct gccacgggcg cggcgcggac
     5881 gagcacgacc tcgcgcccct gctcgacgcg ctggacccgg agcggcggct cgccggcttc
     5941 ctgccccgcg ggccgctctc gctcccgccc ggcggggcgc actggtacgt cgtgcacgag
     6001 gtcgggttcc cggatccggc gacgttcctg ccgacgttcg agacgctctc cggctgggtc
     6061 gacgcggcgc tcgccgacgc gggcgtgccg atcgagcgga ctgtcttcgc ggggttctcg
     6121 caggggacgg tgatgtcgta ctcgctcgcc ttcgccgcgt cgcgaccgcg gccggccggg
     6181 gtgctcgcgt tcagcggctt cgtcccgcgc gtggacgggt tcgagctcga cctcgagggg
     6241 cgcgcgggcc tgccggtctc gatcgcgcac gggacgcacg acccgatcat cacggtcggg
     6301 ttcgggcgcg acgcgcgcga gcggctccag gcggccggcg tggacgtcgc gtacgtcgag
     6361 gagccgatgg cgcactcgat cggccccgcc ggcctcgccc aggcgcgcga ggtgctggag
     6421 cgggcgctcg gcctcgacga gcagccggac tagcgggagg cggcgtcgcc gggacggggc
     6481 tcgtcgagcc ccgagccggg cgtgccgacg ggctcgggcc gcgccgggcg gaggtagagc
     6541 gcctcgtacg cgagcgcggc cgcggcgcct ccgacgagcg ggccgaggta ccagacccag
     6601 aagtcgcccc actccccggc cacgagctgc gggccgaacg cgcgcgccgg gttcatcgcc
     6661 gcgccggtga gcgggccgcc catgaggatg tcgagcgtga tcgtcaggcc gatcgcgagc
     6721 cccgcgatcg cggtgaacgt cccgcgcggg tcggccgcgg tggcgaagat gacccagacg
     6781 aggaagaagg tcaggatcgc ctcgatcacg accccggctc cggctccgat ccccagcccg
     6841 agcgccggcg tcccgagcgc aaccgggtcg gtgagctcgt tcggaagcag gagcgtgagg
     6901 aggatcgcgc cgaggagccc gccgagcatc tgcgccgccc agtagacgcc cgcgagcgac
     6961 ggcgccatgc gcttcgtgac gaggaagccg agcgtcacgg ccgggttgaa gtgcccgccg
     7021 gagatgtgcc cgaccgccga gaccatcacg gcgataacca ggccgtgcgc gagcgcaacg
     7081 ccgacgagcc cgccgtcggc gggcccggcg accgcgatcg acccggcgcc gatgaacacg
     7141 agcgcgaacg cgccgatcgc ctcggcgacg ccgcgctggg cccagtcgcg ctccacggcg
     7201 cggcagcgta cggcgcgcgc cggacggcgc tcggttcgtc tccggcggac gtggcacgtg
     7261 cctggcaccg gtcgtgtccc gcgcggaccc cgtcccgcgc gcgtgagcag gagaaacgcg
     7321 ttcggccgtg tcccgggcgg cctcggccgg tgcctggcac cggtcacgtc ccccgcggac
     7381 gtggacgcgc cggccgggtc aggcgcgcca gtagctgcgc atgagcagca cgaccacggg
     7441 cacgatctcg agccggccga gccacatgag catgctcagg atcccggtcg acaggtcgcc
     7501 gaacggctcg tacgagccga acgggccggc gaagccgaag gccggcccga cgttcccgag
     7561 gcaggccgcg gccgccccga tcgcctcgaa cgtcgccacc tcgacctccc cgcggcgcgc
     7621 gtcgatgagg agcccgagcg tcccgagcgc gaagatcccg aggtagagca cgacgaacgc
     7681 gaacgcggag cggagcgccc gctcgtccac gaccgccccg ctcagccgca ccgggatgac
     7741 cgcctcacgg tgcaccgcct gccggagctc ccgccggatc agcttgacca tgagcaggtg
     7801 ccgcacgacc ttgagcgacc cgcccgtcga ccccgctgag gcgcccacga acatgagcag
     7861 caggagtgtg agcgtcgcga gcgccgtcca gcccgtgtag tccgcggtcg cgaacccggt
     7921 cgtcgtcatg atcgagacgg cctggaagac cgagtcgcgc acggcgccct cgcccaccgc
     7981 gatcccgtcc gcgagcagct cgacgaagag gagcagcgac gccgcgagga ggaacgccgc
     8041 gtagagccgc agctcctcgt cgcgcgcggc gacccggacg tggcgctgga cgaggatcct
     8101 gtagaggcgc aggaagttga tcccggcgag caccatgaag acgacgagga gccactgcgt
     8161 cgccgggacg aacgccgccg ccgactcgtt ctgggtcgag aacccgccga gcgcgagcac
     8221 cgagaaggcg tgcgcgaccg cctggaagag atccatctcg gggtcgatcc cggtccagcc
     8281 gagcagcgcg agcccgagga tgcaggtcgc ggtcaggccg acgtagagcg cccacagccg
     8341 ccgggccgtg tcgcggatcg tcgccgtgag gcgctcgagc tcggtcggcc cggcgagctc
     8401 ggaggagagg agctgccgcc cgccgacccg cagccgcggg agcacggcga cggcgagcac
     8461 gatgatcccc atcccaccga gccagtgggt gagctgccgc cagacgagca tcgagcggtc
     8521 gagcgcctcg atgtccgtga gcacggtcgc ccccgtcgcg gtgaagccgg acacggcctc
     8581 gaagtacgcg ttcaccgggc tcgagagctg ctccacgtcg ccgagcaggt agggcagcgc
     8641 cccgaacgcc ggcacgagga gccagatgag tgcgacgacc aggaacccct cgcgcggccc
     8701 gacctgctcc ctcgtcccgg tgacgagccg gtcgagcgac cacccgacgc ccgccgtcac
     8761 cgcgcccgcg acgacgaacg gccaccacgg ctccccgtac ccgaccgcga ctgccgccgg
     8821 gacgaggaac gcgccgccga ggtacttgag caccccgccg acgaggtcga gcgcgctccc
     8881 gacgtccacg ccgatccgaa gcggcgcgcg gcgcgaccgc agccgtccca agagcgaccc
     8941 gagtccgcgc gagcggcgca cgaggcggaa ggctactccc ctacacggcc gtgaagacgt
     9001 cctcgacggc cggccgcgcc gccccccgga cgaggacgta gacgcggtcg ccggcgagga
     9061 tctccgtgct cccgcggggc gggacggcgg cgaggtcgcg gacgatcacg acgacgaccg
     9121 cgtcggccgg aagcccgagc tcgcgaagcc gcttcccgac gacgcgcgag cgctcgtcga
     9181 cggggaactc gaggacgtcg gcgccgaggc tgcggatcgc cccgacctcg agcggctcga
     9241 cgtgcagcgg ccgcgcctcg gtcgcgagcc cgagccggcg tgcgagcggt tcgagcgtcg
     9301 gcccctgcgt gaccgcggag acgagcacga cgaagaagac ggcgtcgaag atgaggtcgc
     9361 tctcggccac gtccgccgag agcgcgaacg tcgcgagcac gatcggcacg gccccgcgca
     9421 ggcccgccca gccgacgaag acccgctccc gcaccccgag gcccggcacg gggaacggaa
     9481 gcgaggcgag caccgcgagc gggcgcgcga cgacgaggag cacggcggcg agcgcgagcc
     9541 ccggcaggat cacctccgcg aggttggacg ggaagacgag caggccgagg acgacgaaga
     9601 ggacgatctg ggcgaggaac gcgagcccgt ggtggaacgc gacgatcgcg cgcgtgaacg
     9661 gccccggcac gttcccgatg aagaggccca cgatgtacac ggccaggaag ccgctccccc
     9721 cggcgacgtc gacgacgccg aacgagaccg ccgccgtcgc gagcgagagc accggcgcga
     9781 agggcgcgag ctccgccggc agacgcggga agacccaggc ggcgaggagc cccagcgcca
     9841 ggccgagcac gaggccgagc ccgagctgac ggacgaggag gagcgcgagg tcccggccgc
     9901 cgtagccgtc ggtcgtcacc caggcgacga gcccgagggt gagcgcgacc gccatcgggt
     9961 cgttcgcgcc cgactccgcc tcgaggagcg ccgcgagccg ccgccgcagg ctcgtgaagc
    10021 gcagcgtggc gaagaccgcg gccgcgtccg tcgagccgac gacggcgccg agaaggagcg
    10081 cgaccgagag cgacaggtcg tcgaagagca ggtaggcggc gagccccgcg gcgagcgccg
    10141 tgacggcgac gccgaccgtg ctgaggagcg cggccgggac gacgatcggc cggatgtcgc
    10201 gccagcgcgt ggcgagcccg ccctcgaaga ggatcgcgac gaggccgacg atcccgaccg
    10261 tgcgggcgag gtccgggtcg tcgaactcga tcccgccgag cccttcggag ccgagcagca
    10321 tcccgacccc gaggaacgcc acgagcacgg gcacgctggc ccgcgcggcg gtgaaggccg
    10381 tcgcgacgcc cgcgagcagc acgagcccca cgacgagcag gagcgctccc tcgatcgcgt
    10441 tcctcctggt cgggtcggtc gcggcaacct atcgcgcgtc gccggggacg gcggagcgag
    10501 ggcataggat cggcctcccg catgacgctc acgcagctcg ccgtgaccgt ggccctcctg
    10561 ggcgtcctgg gcctgctcgc gagccgcttc ggcctctccg cgattcccgc ctacctcctg
    10621 gccgggctcc tcctcgggcc gaacgagccc gaggcgctca cggtcatccg cccctccgag
    10681 gtgacggagt tcgtcgccga cctcggcgtc atcttcctcc tcttcttcct cgggctcgag
    10741 ttcagcgtcg ggcggcttct gcgatcgggc cgccacttcg gcgcgggagg ctcgatcgac
    10801 ctcgtcgtga acggagggat cgggctcgcg ctcggcctcg cggcgttcgg gccgggcatc
    10861 gccgcggtcg tcttcgcggc cgcggtctac atctcgtcga gcgcggtgac ggtcaagggc
    10921 ctcatcgact tccgccgcct cgcggacgag gagaccgacc tcgtcctcgc gatcctcatc
    10981 ttcgaggacc tcgtgatcgc gttcgttctc ggcttcgcgg cgacgggcgg cggcggcttc
    11041 gggccgacgt tcgtcctcgt ggcgaaggcg ctcgccttcg tcggcctcac gctggtcgcc
    11101 tcgcgctacg cctctcgggc gatcgcgatg acgctcgagc ggatgccgcg cgagttcttc
    11161 ctcctcttca gcttcgcgct cctcatcggg atgagcgcgg tcgccgagcg gctcgggctc
    11221 tcggaggcga tcggcgcgct catggccggc gtcgtcctct ccgagacggc cgtccgggcg
    11281 gagctcgagg agcggttctt cagcttccgc gacctgttcg cggcgctctt cttcttcgtc
    11341 ttcggcctca cgatcgacct cgatgcgctc ggaagcgtgg gctggctcgt cgccgtcgcg
    11401 gtcgtcctca ccgtggccgg gaagctcttc gcgggctacg cggccgggca ggtcggaggg
    11461 ttcacacgcg gccagagcct gaacaccggc gccgccctcg tcgcccacgg cgagttcacc
    11521 gtgatcatcg cccagctcgc ctcgcgcaac ccgcagatct cgccgacgac gcgctccgac
    11581 ctcgtcgcgt tcgcggggct ctacgtcctc gccacggcca cgatcggcgt cgtgctcatg
    11641 aaggagtcga agcggatcgg ccggaggctc ttccggagac ctacactgac acaggaggct
    11701 ctcgagtgaa tcaggtcacc cagacccgcc tgcccggcgt cgggacgaag ttctcgatcc
    11761 cgacctcgca cggcggccgc ctcgccgtca tccagcacaa cgacggcatg cgcgagatct
    11821 acttcttcca gcgccgcgac gacgacgagc cgtgcgcggt catccagctc gaggacgacg
    11881 aggcccgcca gctcggcgcg gtgatcggcg gcgcgtacga gcggccgcgc atcgtcgagg
    11941 agctcgaaat ggccttcgga gagctcgcga tcgaatgggt ccccgtgccg cagggcagcc
    12001 cggcgatcgg gcattcgctc gccgagtgcg cattccgcgc gcggacgggg atcacgatca
    12061 tcgccatcct ccgcgagccg gagccggtca cgggcgccca gcccggcgac gtgatcaagg
    12121 agggcgacac gctcgtgacg gtggggaagc tcgggcagta caccgcgttc cgcaagctcc
    12181 tcgccgaggg ccccttcccg gccccgaacc aggagccgac ccacatcccg aagcggctct
    12241 agatggctga tcgtgatccg ctggcgaatc ccggggtcgg atgacataac accgatccgg
    12301 gaggtgcccg ttgcgctcgt ccggcctgac atcggccgtc cgtggccgat atcagggggt
    12361 ccggtgtggg ccgagtaccg gggccgctac tccgcggtga aggcggcgta ctgcttgtac
    12421 gtcccgtagt ggcccgtgat gtcgcagagc aggatgtact ccccggcctc gaggtccacg
    12481 gtcagctcgg cgccccagcc cccaccgcgg atgtggtggt ctgcagcgcc gggctcgctg
    12541 tccgagggaa cgatcggcgg cgtcgcgtag gcggcttcgc cgacgatgtt ctcctcgacg
    12601 aggtccgctt tgtcctcctc agtgatcgga agatcgccgg gctcgacacc gtcgagattg
    12661 gagtagacga tgaactcgtg gtagagctca cccatgttgg tgacggcgaa cgtcaccgtg
    12721 ccggcttcca ctgtcgtttg tgcgggctcg acgtaatacg tcgaacccga ctcacccagc
    12781 tcgacctcga cgacctcgcc ctcggcagcg acgtcgccgt catcaccgcc acacgccgcc
    12841 acactgaata gcagcacggc gacgcccagc agcaccagaa gcatccgggt tcgtctcacg
    12901 accttgtccc ctggatcagt acctgagcga ctgatcgcgg aatcgaaaat ccagggtagt
    12961 cgccctagca gcgccgcgaa tcagtagaaa gccgacccta gccaacggac atgtacggaa
    13021 atcccgcggt ggacccgtgc cgctcacaac caccgctcgc ggcgttagag acgcaccagg
    13081 aacggcgcac gaggcttctg aaaccggttc taaagcacct tctcgacgtt cgcgacgcgc
    13141 gacgactcgg tgaagatgat cgcgcggtcg cccgggagga gcacgtcctc gccgtgcggg
    13201 aagatcgcct cgccgtcgcg gacgatcgcg ccgatcagcg agccggtcat cgggagctcg
    13261 cggaagggga tcccgcagag ctcgctctcc tcccggacgg tgatgtcgag cacctcgaac
    13321 cggtcgccct cgagcatcgc gagctggcgg atgcgcgggt cgtgcgcgaa gcgcaccatc
    13381 tcctccgcgg tcaccgagcg cgggttcacc gcagcgtcga cgccggcccg ctcgaagacg
    13441 agctcggcgc ccggctcgtg gacgatggcg accgtgaacg cgacggcgtg gagcttcgcg
    13501 agcgtcgcgg cgtagaggtt cttcgcgtcc tcccgcatgg cgaagacggc gacctgcgag
    13561 cggccgatcc gctcgcgctc gaggaactcc gggtcgaccc cggtcgcatg gaagacggcg
    13621 acaccgggga gcgcctcggc cacctcgcgc gcccgctccc cgtcggcctc gatcaggcgg
    13681 acgccgattc cctggtcggt gagcgtccgc gcgacctcga agccggtctg gcccccgccg
    13741 aagacgacga cgtcgtcgac caccgccgtc cggccgcgga gcatgagccg gctccactcc
    13801 ctggccgcgg cgggtgagcc gatgatcacg acccggtcgc cccggaggac ggactcgtcc
    13861 ccgcgcggga cgagcacgct gtcgccgcgg atgatcgccg ccaccttcga ctcgtccggg
    13921 atccgcgcct cgcggagcgg gcggccgatc acctcgccgt cgtccccgcc gggctcgacc
    13981 tcgacgtcga gctcgatcat ctggacgagc ccgtccgcga agacgtccgt ctgccgcgcc
    14041 gcgggcacgc cgacgagccg ggcgatcgcg tgcgccgcct cgagctccga gcagaccatg
    14101 aagtcgatct cgagctcccg ctcgcgccag atctcgaggt actcgacgtt cgtcg
//
LOCUS       DASTFC010000085         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_298163,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000085 DASTFC010000000
VERSION     DASTFC010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298163"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFO88_02565"
     CDS             <1..822
                     /locus_tag="VFO88_02565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET9544435.1"
                     /translation="ELLPRFGDGSFALVYADPPFNTGSRRVRRTLETRAHPNGDRRGF
                     AGRRYRTRVLAESSYADRFDDYLGFLEPRLREIRRVLAESGTLYLHLDYREAHGAKLL
                     CDEVFGRDCFLNEIVWAYDYGARTKRRWPAKHDTILVYVKHPERYWFDAEAVDREPYM
                     APGLVGPEKAARGKLPTDVWWHTIVSPTGREKTGYPTQKPVGLVRRMVQASTRPGETC
                     LDPFAGSGTLGAAARELGRGFVLVDESPEAVEIAAARLGLAAVVVRGSAPLEARP"
     gene            917..1336
                     /locus_tag="VFO88_02570"
     CDS             917..1336
                     /locus_tag="VFO88_02570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544436.1"
                     /translation="MTDPYSSGVVALRIPAKPEYIALARLALSGLAQVRPVEPETLAD
                     LKLALTEACSNSVRHAYREGRDGTVDVRYELNGSELAVEVVDEGGGFDVDATVDGELG
                     ELDEGGLGLAIIRALTDELAIDSHERGSRLRFAKKLP"
     gene            1370..>2079
                     /locus_tag="VFO88_02575"
     CDS             1370..>2079
                     /locus_tag="VFO88_02575"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544437.1"
                     /translation="MQLQPVNVGHKALADYATIASRGVMDQIRTLAEALQGKRVLHLS
                     ATAFGGGVAEILYTLVPLMRDAGLETEWQVINGQDEFFDVTKSIHNALQGADVTLTDE
                     QKKIFHAYNRANAEALDGAWDFVIVHDPQPAAILDGGRPGGGRWIWRCHIDLSAPSRD
                     VIDFLAPSLARYDAAVFHRREYVPAGIPLPRAYIWPPAIDPLAPKNMALSPEDAAYIV
                     DQFGIDVERPLITQVSRF"
BASE COUNT          302 a          780 c          703 g          294 t
ORIGIN      
        1 gagctcctcc cgcgcttcgg cgacggctcg ttcgccctgg tctacgccga cccgcccttc
       61 aacacgggct cccgtcgcgt gcggcgcacg ctcgagaccc gcgcccaccc gaacggcgac
      121 cgccggggct tcgccggccg ccgctaccgc acccgcgtcc tcgccgagtc gtcgtacgcc
      181 gaccgcttcg acgactacct cggcttcctc gagccgcgcc tgcgcgagat tcgccgcgtc
      241 ctcgccgaga gcgggacgct ctacctccac ctcgactacc gcgaggcgca cggggcgaag
      301 ctcctctgcg acgaggtctt cggccgggac tgcttcctga acgagatcgt ctgggcgtac
      361 gactacggcg cgcgcacgaa gcgccgctgg ccggcgaagc acgacacgat cctcgtctac
      421 gtcaagcatc ccgagcgcta ctggttcgac gcggaggcgg tcgaccggga gccctacatg
      481 gcgcccggcc tcgtcgggcc ggagaaggcc gcgcgcggca agctccccac ggatgtctgg
      541 tggcacacga tcgtctcccc gaccgggcgg gagaagaccg gctacccgac ccagaagccc
      601 gtcggcctcg tgcgccggat ggtgcaggcg tccacgcgcc cgggcgagac gtgtctcgat
      661 ccgttcgccg gcagcggcac cctcggggcc gccgcgcgcg agctcgggcg cggcttcgtg
      721 ctcgtcgacg agagccccga ggcggtcgag atcgcggctg cccggctcgg cctcgcggcg
      781 gtcgttgtgc gaggatcggc gccgctcgag gcaagaccat gaccgaccac gaccgccttt
      841 cctgtttcgc cgcgcccgat gggggaatac ggcccgagtg gccgcaggac gacatgagac
      901 ctgaacgcgg ccctctatga ccgaccccta ctcctccggc gtcgtcgcac tccgcattcc
      961 ggccaagccg gagtacatcg cgcttgcccg tctcgccctg agcggcctcg cccaggtgcg
     1021 cccggtcgag cccgagacgc tcgccgacct gaagctcgcc ctgacggagg cctgctcgaa
     1081 ctcggtgcgc cacgcgtatc gcgaggggcg cgacggcacg gtggacgtcc gctacgagct
     1141 gaacggctcc gagctggccg tcgaggtggt ggacgaggga ggcgggttcg acgtcgacgc
     1201 gacggtggac ggcgagctcg gcgagctcga cgagggcggc ctggggctcg ccatcatccg
     1261 ggcgctcacc gacgagctcg cgatcgactc gcacgagcgc ggctcccggc tccgcttcgc
     1321 gaagaagctc ccgtaggcgc tcgcaggtcc cccaggtaat ctccgccagt tgcagctcca
     1381 gccggtcaac gtggggcaca aggccctcgc cgattacgcg acgatcgcga gccgcggcgt
     1441 catggaccag atccggacgc tcgcggaggc gctccagggg aagcgggtgc tgcacctctc
     1501 ggcgaccgcc ttcggcggcg gcgtcgcgga gatcctctac acgctcgtcc cgctcatgcg
     1561 cgatgccggg ctcgagaccg agtggcaggt gatcaacggg caggacgagt tcttcgacgt
     1621 gacgaagtcg atccacaacg cgctccaggg cgccgacgtc acgctgacgg acgagcagaa
     1681 gaagatcttc cacgcctaca accgtgcgaa cgccgaggcg ctcgacggcg cctgggactt
     1741 cgttatcgtc cacgacccgc agccggccgc gatcctggac ggcggtcggc cggggggcgg
     1801 gcggtggatc tggcgttgcc acatcgacct ctcggccccg agccgggacg tgatcgactt
     1861 cctcgcgccc tcgctcgccc gctacgacgc cgcggtgttc caccggcgcg agtacgtgcc
     1921 ggccgggata ccgcttccgc gcgcctacat ctggcccccg gcgatcgacc cgctcgcgcc
     1981 gaagaacatg gccctctcgc ccgaggacgc cgcgtacatc gtcgaccagt tcgggatcga
     2041 cgtcgagcgg ccgctcatca cccaggtttc ccgcttcga
//
LOCUS       DASTFC010000086         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_957376,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000086 DASTFC010000000
VERSION     DASTFC010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957376"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1831
                     /gene="treS"
                     /locus_tag="VFO88_02580"
     CDS             170..1831
                     /gene="treS"
                     /locus_tag="VFO88_02580"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET9544438.1"
                     /translation="MSAEPRHWFESNPLWFKTAVFYEIHVRGFFDGSDDGSGDFRGLS
                     EKLDYLQWLGIDCVWLLPFYPSPLRDGGYDIADFGGVHPDYGTTEDFRAFVEAAHERG
                     IRVIADLVMNHTSSDHPWFQEARQGRDNPKHDWYVWADDEEGYPDARIIFLDTETSNW
                     TWDPACERYYWHRFFSHQPDLNYENPEVQEAMLDVLRFWLDLGLDGFRLDAVPYLYEE
                     EGTNGENLPATHEFLKRVRAEVDSHYDDRVLLAEANQWPEDVVDYFGDGDECHMAFHF
                     PVMPRMFMAVRREEARPILDILRNTPAIPDTCQWGLFLRNHDELTLEMVTDEERDYMY
                     AEYAKEPRMRLNLGIRRRLAPLLDNSRDEIELLTAILFSLPGSPVLYYGDEIGMGDNV
                     YLGDRDGVRTPMQWTGDRNGGFSRADFAQLFLPPLMDPVYGYQAVNVESQLRQPHSLL
                     RWVQRFVGLRKAHPVFGLGSFEALVPENPRVFAHIRTYEEDVILCVHNLARSAQAVEL
                     DLRWYDGYVPEEMFGHSRFPPIGELPYLLTFAPRGFFWFKLRAPA"
     gene            1828..3243
                     /locus_tag="VFO88_02585"
     CDS             1828..3243
                     /locus_tag="VFO88_02585"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9544439.1"
                     /translation="MIAAIVERLGEERLAACVAEARWFGAKTRDVTGARVVAAPLVRE
                     EAAGLVLALVEVRFETGSHEVYQVPLGVRPAGEWDEGVVGEVDGSVVYDALTDPDLVG
                     ALVGLMREGAAIEDEEEDAHIELSAVDAGADLLTGPWPVRAMAVEQSNTSVVLGEELV
                     LKVYRRLEAGVNPELELLRFLTARGFPNVPALAGWYAYSGRLMDATLGILQRYVAVEA
                     DGWAFALDALADDPERFLAEARRLGEVTGHMHATLASDSTDPAFAPEEPSAESLGLLT
                     ATIDEEIAGAFASLPELDALEPIAGRGEEVREQLRLVSHAGPLGRTIRHHGDYHLGQV
                     LWTGADWIVIDFEGEPARPLAERRRKRSALRDVAGMLRSFAYVATAAELTRGSPAPED
                     WEERAREEFLAGYLPVVEPLGLLPAGHAQTSRLLAVFELEKAVYELRYELDHRPDWVS
                     IPVAGIVRLLESDALSAPVRP"
     gene            3249..>4487
                     /gene="glgB"
                     /locus_tag="VFO88_02590"
     CDS             3249..>4487
                     /gene="glgB"
                     /locus_tag="VFO88_02590"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HET9544440.1"
                     /translation="MSDLERVVARDHHDPHTVLGAHPDERGVRVRAFLPNAVRVSAAV
                     DGALYELERVHDEGLFEGVVEDAELPLAYELEVAYDGTTLRLRDPYSFPPTLGDLDLH
                     LAGEGRHERLYERLGAHVREHEGVAGVAFAVWAPAARSVSVVSDLNLWDGRVHQMRSL
                     GSSGVWELFVPAAEPGARYKYEVRGADGALRLKSDPLAFATEVPPANASVVHEPRHEW
                     RDDEWLARRRASSAHAEPMSIYEVHLGSWRLNPLEGNRPLTYVELADELADYVLDLGF
                     THVELLPVMEHPFSGSWGYQVTGFYAPTSRFGSPDDFRAFVDRLHVRGLGVLLDWVPA
                     HFPTDEFALARFDGTALYEHEDPRRGFHPDWGTLVFNYGRNEVRNFLLANALFWLREY
                     HADGLRVDAVASMLYLDYSRE"
BASE COUNT          598 a         1626 c         1592 g          671 t
ORIGIN      
        1 ttcgcgccgc aggagggcgt cgtcgtgatc ccggccgagc tcgggctgcc gccggcgttc
       61 cgcgcccgcg agctgctcac ggacgcgacg ttcgactggc ggatcggacg gaactacgtg
      121 cgcctcgacc ccgccgcgcc ggcccacgtc ctgcgcatcc cggcctagcg tgagcgccga
      181 gccccgccac tggttcgagt cgaacccgct gtggttcaag acggccgtct tctacgagat
      241 ccacgtgcgc gggttcttcg acggctccga cgacggcagc ggcgacttcc gcggcctgtc
      301 cgagaagctc gactacctgc agtggctcgg gatcgactgc gtgtggctcc tcccgttcta
      361 tccctcgccg ctgcgcgacg gcggctacga catcgcggac ttcggcggcg tgcaccccga
      421 ctacgggacg acggaggact tccgcgcgtt cgtcgaggcg gcgcacgagc gcgggatccg
      481 cgtgatcgcc gacctcgtca tgaaccacac gtcgagcgat cacccctggt tccaggaggc
      541 gcggcagggc cgcgacaacc cgaagcacga ctggtacgtc tgggccgacg acgaggaggg
      601 ttacccggac gcgcggatca tcttcctcga caccgagacc tcgaactgga cgtgggaccc
      661 ggcgtgcgag cgctactact ggcaccggtt cttctcccac cagcccgacc tgaactacga
      721 gaaccccgag gtgcaggagg cgatgctcga cgtcctgcgc ttctggctcg acctcgggct
      781 cgacgggttc aggctcgacg ccgtcccgta cctctacgag gaggagggga cgaacggcga
      841 gaacctcccg gcgacgcacg agttcctgaa gcgcgtgcgc gcggaggtgg actcgcacta
      901 cgacgaccgc gtgctcctcg ccgaggcgaa ccagtggccc gaggacgtgg tcgactactt
      961 cggcgacggc gacgagtgcc acatggcctt ccacttcccg gtcatgccgc ggatgttcat
     1021 ggccgtgcgg cgcgaggagg cgcgtccgat cctggacatc ctccgcaaca cgcccgcgat
     1081 cccggacacg tgccagtggg gcctcttcct gcgcaaccac gacgagctga cgctcgagat
     1141 ggtcaccgac gaggagcgcg actacatgta cgccgagtac gcgaaggagc cgcgcatgcg
     1201 cctgaacctc gggatcaggc gccggctcgc gccgctcctc gacaactcgc gggacgagat
     1261 cgagctcctg acggcgatcc tcttctcgct cccgggcagc ccggtcctct actacgggga
     1321 cgagatcggg atgggcgaca acgtctacct cggcgaccgt gacggggttc ggactccgat
     1381 gcagtggaca ggcgaccgga acggaggctt ctcgcgtgcg gacttcgcgc agctcttcct
     1441 gcccccgctc atggatcccg tgtacggata tcaggccgtg aacgtcgagt cacagctccg
     1501 gcagccgcat tcgcttctcc gctgggtcca gcgcttcgtc gggctgcgga aggcgcaccc
     1561 ggtcttcggc ctcggctcgt tcgaggcgct cgtcccggag aacccgagag tcttcgcaca
     1621 catccgcacg tacgaggagg acgtgatcct ctgcgtccac aacctcgcgc gctcggcgca
     1681 ggcggtcgag ctcgacctgc gctggtacga cgggtacgtg cccgaggaga tgttcggcca
     1741 ctcgaggttc ccgccgatcg gcgagctgcc gtacctcctg acgttcgcgc cgcgcgggtt
     1801 cttctggttc aagctgaggg ccccggcgtg atcgcggcga tcgtcgagcg gctgggcgaa
     1861 gagcggctcg cggcgtgcgt cgccgaggcg cgctggttcg gcgcgaagac gcgcgacgtg
     1921 accggcgcgc gcgtggtcgc cgcgccgctc gtccgcgagg aggcggccgg gctcgtcctc
     1981 gccctcgtcg aggtgcgctt cgagaccggc tcgcacgagg tctaccaggt gccgctcggc
     2041 gtccggcccg ccggggagtg ggacgagggg gtcgtcggcg aggtcgacgg ctcggtcgtc
     2101 tacgacgcgc tcaccgatcc ggacctcgtc ggcgcgctcg tcgggctcat gcgcgagggc
     2161 gcggcgatcg aggacgagga ggaggacgcg cacatcgagc tctcggccgt tgacgccggc
     2221 gcggacctcc tcaccggccc ctggccggtc cgcgcgatgg cggtggagca gtcgaacacg
     2281 tcggtcgtcc tcggcgagga gctcgtcctc aaggtgtacc gccggctcga ggcgggcgtc
     2341 aacccggagc tcgagctgct ccgcttcctc accgcgcgcg ggttcccgaa cgtgccggcg
     2401 ctcgcgggct ggtacgcgta ctcggggcgg ctcatggacg cgacgctcgg gatcctccag
     2461 cgctacgtcg ccgtcgaggc ggacggctgg gcgttcgcgc tcgacgcgct cgccgacgac
     2521 ccggagcggt tcctcgccga ggcgcggcgg ctcggcgagg tcacgggcca catgcacgcg
     2581 acgctcgcct cggactcgac cgacccggcc ttcgcgccgg aggagccgag cgcggagagc
     2641 ctcggcctgc tcaccgcgac gatcgacgag gagatcgccg gcgcgttcgc ctcgcttccg
     2701 gagctcgacg cgctcgagcc gatcgccggg cgcggggagg aggtgcgcga gcagcttcgc
     2761 ctcgtctcgc acgcgggccc gctcgggcgc acgatccgcc accacgggga ctaccacctc
     2821 ggccaggtgc tctggacggg cgcggactgg atcgtgatcg acttcgaggg cgagcccgcg
     2881 cgcccgctcg ccgagcggcg gcgcaagcgc tccgccctcc gggacgtggc cgggatgctc
     2941 cgctcgttcg cgtacgtcgc caccgcggcc gagctgacgc gcggctcgcc cgcgccggag
     3001 gactgggagg agcgggcgcg ggaggagttc ctcgcggggt acctccccgt cgtcgagccg
     3061 ctcgggctcc tgcccgccgg gcacgcgcag acgtcgcggc tcctcgccgt gttcgagctc
     3121 gagaaggccg tgtacgagct ccgctacgag ctcgaccacc ggccggactg ggtgtcgatc
     3181 ccggtcgcgg ggatcgtccg gctgctcgag tcggacgcgc tctcggcacc ggtgcggcct
     3241 tgaccgtcgt gagcgacctc gagcgcgtcg tcgcgcgcga ccaccacgac ccgcacacgg
     3301 tcctcggtgc ccatccggac gagcgcggcg ttcgggttcg ggccttcctg ccgaacgcgg
     3361 tgcgcgtctc cgccgccgtc gacggtgcgc tctacgagct cgagcgcgtg cacgacgagg
     3421 ggctcttcga gggcgtcgtc gaggacgccg agctgccgct cgcctacgag ctcgaggtgg
     3481 cctacgacgg cacgacgctg cggctgcgcg acccgtactc gttcccgccc acgctcggcg
     3541 acctcgacct ccacctcgcc ggggaggggc ggcacgagcg cctgtacgag cggctcggcg
     3601 cgcacgtccg cgagcacgag ggcgtcgcgg gggtcgcgtt cgccgtctgg gcgccggccg
     3661 ctcgctcggt cagcgtcgtc tccgacctga acctctggga cggccgcgtg caccagatgc
     3721 gctcgctcgg gtcgagcggc gtgtgggagc tcttcgtccc ggccgccgag ccgggggcgc
     3781 gctacaagta cgaggttcgc ggcgccgacg gggctcttcg tctcaagtcc gacccgctcg
     3841 ccttcgcgac ggaggtgccg cccgcgaacg cctcggtcgt ccacgagccg cgccacgagt
     3901 ggagagacga cgagtggctc gcgcgccgcc gggcgagctc cgcgcacgcc gagccgatgt
     3961 cgatctacga ggtgcacctc ggctcgtggc ggctgaaccc gctcgagggc aaccgcccgc
     4021 tcacgtacgt cgagctggcc gacgagctcg ccgactacgt cctcgacctc gggttcacgc
     4081 acgtcgagct gctccccgtc atggagcacc cgttcagcgg ctcgtggggc taccaggtga
     4141 ccgggttcta cgcgccgacg tcacgcttcg gctcgcctga cgacttccgc gcgttcgtcg
     4201 accggctgca cgtgcggggg ctcggcgtgc tcctcgactg ggtgccggcg cacttcccga
     4261 cggacgagtt cgcgctcgcc cgcttcgacg ggacggcgct ctacgagcac gaggacccgc
     4321 gccgcggctt ccacccggac tgggggacgc tcgtcttcaa ctacgggcgg aacgaggtgc
     4381 ggaacttcct cctcgcgaac gcgctcttct ggctgcgcga gtaccacgcg gacggcctgc
     4441 gcgtggacgc ggtcgcctcg atgctctacc tcgactactc gcgcgag
//
LOCUS       DASTFC010000087         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_149720,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000087 DASTFC010000000
VERSION     DASTFC010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149720"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1881
                     /gene="secF"
                     /locus_tag="VFO88_02595"
     CDS             <1..1881
                     /gene="secF"
                     /locus_tag="VFO88_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET9544441.1"
                     /translation="AIALQSFAIVLDKEIRSFPTIDFVGDTATGIAGGRAQISGLEGT
                     QEARDLALVLQTGALPYSFEQLERTDISATLGEESLREALIAGIGGLIAVALFLLLVY
                     RFLGVVAIVGLGIYGVFLYGTILALNVTMTLPSFAGIILTIGVAADANIVIFERIKEE
                     VRAGKSVRAAIAGGYRKGFGTIVDANVVTMITAAVLFLVGTGSVRGFALMLLLGVLIS
                     MVTAVLATRALLGILGRFRWFDNPSFMGASAQKIPAWQRIDIVGRRRTWFTIAGVVLA
                     IAVGSLIFKGLNLGIDFRGGSQVTFETPQAHAVADVREEMEALGIGEAVVQGRGEAGD
                     DGGYESFQMKTESLTADEQRAVERTLEEEFEVESVSVRNVSGSFSEQILRGAIIAIVI
                     SLILIIIYVSFRFEYKFALPIFRALFYDVLVAMGVYSIVGWEVTAATVAAILTILGYS
                     MYDTIIIFDRVRENMQLMKKSSFQAIANQSLWETIRRSLATTFITLLPITSLILFGGD
                     TLKEFAFALLIGIGSGAFSTIFIATPFLATLKEREPEWERRKDAGLREKLELSEPVEQ
                     EPEPEPVAVAAPAPTDGGDGGDVTAERETNGEADGGRSAAAARREARRKRRRARPHGR
                     SR"
     gene            1865..2218
                     /locus_tag="VFO88_02600"
     CDS             1865..2218
                     /locus_tag="VFO88_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544442.1"
                     /translation="MAGAADDRERGLAEELLFAGVGAVSLTKARADELVDELVQRGRL
                     TRADARELVDDMTGRWRGEAGRVGERASASLAGVMRELGLVTRNEWEELELRLAQVEH
                     RLKLLETPAEPPRPV"
     gene            2254..2379
                     /locus_tag="VFO88_02605"
     CDS             2254..2379
                     /locus_tag="VFO88_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544443.1"
                     /translation="MFRPYRRGNVRVYGCSPGCLALSLGISILLTILVNACIRIV"
     gene            2411..>2637
                     /locus_tag="VFO88_02610"
     CDS             2411..>2637
                     /locus_tag="VFO88_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544444.1"
                     /translation="MSSRPAVRQLGRLSEIAQVAAKHGFGYLFDSRRRDGGPSEREME
                     VVADETSTRGVRLRAMLDELGPTFVKFGQLLS"
BASE COUNT          367 a          946 c          920 g          404 t
ORIGIN      
        1 gcgatcgcgc tccagagctt cgcgatcgtc ctcgacaagg agatccgctc gttcccgacg
       61 atcgacttcg tcggcgacac ggcgaccggc atcgccggcg gccgcgcgca gatcagcggc
      121 ctcgagggca cgcaggaggc gcgcgacctc gcgctcgtcc tgcagaccgg cgccctgccg
      181 tactcgttcg agcagctcga gcgcacggac atctccgcga cgctcggcga ggagtcgctc
      241 cgcgaggcgc tcatcgcggg catcggcggc ctcatcgcgg tcgcgctctt cctgctcctc
      301 gtctaccgct tcctcggcgt cgtcgcgatc gtcggcctcg gcatctacgg cgtcttcctc
      361 tacgggacga tcctcgcgct caacgtgacg atgaccctgc cgagcttcgc cgggatcatc
      421 ctcacgatcg gcgtcgcggc cgacgcgaac atcgtcatct tcgagcgcat caaggaggag
      481 gtccgagccg gaaaatcggt gcgggccgcc atcgcgggcg gctaccggaa gggcttcggg
      541 acgatcgtcg acgcgaacgt cgtgacgatg atcacggcgg ccgtcctctt cctcgtcggc
      601 accgggagcg tgcgcgggtt cgcgctcatg ctcctgctcg gcgtcctcat ctcgatggtc
      661 acggccgtcc tcgcgacgcg cgccctcctc gggatcctcg ggcgcttccg ctggttcgac
      721 aacccgtcct tcatgggcgc gagcgcgcag aagatccccg cctggcagcg gatcgacatc
      781 gtcgggcggc ggcgcacgtg gttcacgatc gcgggcgtcg ttctcgcgat cgcggtcggc
      841 tcgctgatct tcaagggcct gaacctcggg atcgacttcc gcggcggcag ccaggtgacg
      901 ttcgagacgc cccaggcgca cgcggtcgcc gacgtgcgcg aggagatgga ggcgctcggg
      961 atcggcgagg cggtcgtcca gggccgcggc gaggcgggcg acgacggcgg ctacgagtcg
     1021 ttccagatga agacggagtc cctcaccgcg gacgagcagc gggcggtcga gcggacgctc
     1081 gaggaggagt tcgaggtcga gtcggtcagc gtcaggaacg tctcgggaag cttctcggag
     1141 cagatcctgc gcggcgcgat catcgcgatc gtcatctcgc tgatcctgat catcatctac
     1201 gtctcgttcc ggttcgagta caagttcgcg ctcccgatct tccgggcgct cttctacgac
     1261 gtgctcgtcg cgatgggggt ctactccatc gtcggctggg aggtgacggc cgcgaccgtg
     1321 gcggccatcc tcaccatcct cggctactcg atgtacgaca cgatcatcat cttcgaccgc
     1381 gtgcgcgaga acatgcagct catgaagaag tcgagcttcc aggcgatcgc gaaccagtcg
     1441 ctctgggaga cgatccgccg ctccctcgcg acgacgttca tcacgctcct tccgatcacg
     1501 tcgctcatcc tgttcggcgg cgacacgctg aaggagttcg cgttcgcgct cctcatcggg
     1561 atcggctcgg gcgcgttctc gacgatcttc atcgccacgc cgttcctcgc gacgctcaag
     1621 gagcgcgagc ccgagtggga gcgccgcaag gacgcggggc tccgcgagaa gctcgagctc
     1681 agcgagcccg tcgagcagga gccggagccc gagccggtcg cggtcgccgc gccggcaccg
     1741 acggacggcg gggacggcgg ggacgtcacg gccgagcgcg agacgaacgg cgaggccgac
     1801 ggcggacgga gcgccgccgc ggcccgacgg gaggcgcgcc gcaagcgccg ccgggcgagg
     1861 ccgcatggcc ggagccgctg acgaccgcga gcgcgggctc gccgaggagc tgctcttcgc
     1921 gggcgtcggc gccgtctcgc tgacgaaggc gcgcgcggac gagctcgtgg acgagctcgt
     1981 gcagcgcggc cggctcaccc gcgcggacgc acgcgagctc gtggacgaca tgaccggccg
     2041 gtggcgcggc gaggccgggc gcgtcggcga gcgggcctcc gcgagcctcg ccggcgtcat
     2101 gcgcgagctc ggcctcgtca cgcgcaacga gtgggaggag ctcgagctcc gcctcgccca
     2161 ggtcgagcac cgcctcaagc tgctcgagac gccggccgag ccgccgcgcc cggtctgacg
     2221 tttccggcgg gcccctccgg gtagccccgg ccgatgttcc gtccgtaccg ccggggcaac
     2281 gtccgcgtct acggctgcag ccccggttgc ctggccctct cgctcggcat ctcgatcctg
     2341 ctcacgatcc tcgtgaacgc ctgcatccgg atcgtctgag ccgcccgcgc gcgcccgtag
     2401 actgcgccgc atgagctccc ggcctgccgt gaggcagctc ggccgtctct ccgagatcgc
     2461 gcaggtcgcg gcgaagcacg gcttcggcta cctcttcgac tcccgccggc gcgacggggg
     2521 cccgagcgag cgcgagatgg aggtcgtcgc cgacgagacc tcgacccgcg gcgtccgcct
     2581 ccgcgcgatg ctcgacgagc tcggcccgac gttcgtgaag ttcggccagc tgctctc
//
LOCUS       DASTFC010000088         6696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1188092,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000088 DASTFC010000000
VERSION     DASTFC010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188092"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFO88_02615"
     CDS             <1..531
                     /locus_tag="VFO88_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET9544445.1"
                     /translation="PRSGMLVLRVPGESYAAAMDALGRLGEIDHESEEGTDVSQEYVD
                     LEARQRHLEAVEAQLLELLERARTVQAALAVQSQLNGTQLELEQVRGRLRFLDDRTEF
                     ATITLELREPEREEADAGGWGVVDAWKAGAHAFVTVARWAFVLVAAVAPLLLVAVLGW
                     LGARAVRRRRAAAPTG"
     gene            584..1036
                     /locus_tag="VFO88_02620"
     CDS             584..1036
                     /locus_tag="VFO88_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544446.1"
                     /translation="MAKQENDTGVLNRLAGRGEEAVNRLMEELGKNEKVTDALARAME
                     AKGKVDERTRKTLGTVGLAAADQLEGLRNQIEALEKRLAALEKTEAGKAGETAATARS
                     TTAAKPKTTAARPKPKATPETPKTTAARKPPTTRKSPPPPTDEPTGSG"
     gene            complement(1033..1959)
                     /locus_tag="VFO88_02625"
     CDS             complement(1033..1959)
                     /locus_tag="VFO88_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9544447.1"
                     /translation="MARDGVVFLVNPASANGSTGRRWAEIAHRAAAAGLEGEALISTR
                     QGEIAELAERAVADGAAVIVVVGGDGTVYEAVNGLIRSGASAEVDVAIVPRGTGTDFV
                     RTFGITGDLDRAFATAREGRVRAVDVGRARYVAWDGTPAESNFANFAGAGISGAIARR
                     ANATSKAAGGRVSFMWATIAVFAGWKATPVELTVDGERRAGPMFEVLATIGEYAAGGM
                     RVTPGAAPDDGLLDVITIGDVTKIDFVLTFPKIYRGTHVRHPKIETLRGRTVRIESEE
                     PLPIALDGEQPGTTPLEVDLLPGALRLRVPAR"
     gene            complement(1965..2678)
                     /locus_tag="VFO88_02630"
     CDS             complement(1965..2678)
                     /locus_tag="VFO88_02630"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HET9544448.1"
                     /translation="MPIHVRAEPGDYAEACLLPGDPLRARYIAETFLDDVRQTNAERG
                     MLGFTGTFQGRPVSVQSTGMGCPSAGIVIEELVQLGVKRLLRVGTSGGLQPDLELGDL
                     IIALSAVPADSTATHYVGGEPHAPTANWELVHGAAHAAKELGKRVRVGPIVSSDVFYD
                     PDQGRAQRWSDRGILAVEMEAAVLFTLGALRKVQAGCLLTVSDVIVEGEFKRISDEDL
                     KAAVDGMTELALGTVTAER"
     gene            2735..3520
                     /locus_tag="VFO88_02635"
     CDS             2735..3520
                     /locus_tag="VFO88_02635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9544449.1"
                     /translation="MRRLVRRPDSKRILAFCAEEPVERVFLEDVARRGLGRFAALEED
                     AGYVAVCHIGANVVPSGAGTAAFASLAAEAEPRMIVGEEGAVSELWDAVRVALPTPLD
                     DRPGQPVYELREPPPPGESGLRAATFDDLELLVPAAAAAYLEEVGIDAYERDPALFEW
                     RTRVQVEQGRSWLWRRDGEILFKAEASAWTDRAVQLQQVWVDPRLRGHGYAKRGLADL
                     CRLLLERTPIVCLFVRPENTPALALYDSLGMRRTIAYRSLIFP"
     gene            3608..4057
                     /locus_tag="VFO88_02640"
     CDS             3608..4057
                     /locus_tag="VFO88_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544450.1"
                     /translation="MTAAALVLAFGIGFAMRGGGGEAAYAHSCSATDKRFIQAASTDM
                     TALAVWADGYRAGELDADEVARQASDAARRVGYVKPRDPSLKLSQRLINAMFVEYGEA
                     VSVGKKEKKRAGLHMHRAYGLANFAREVLVQAQPGLAREGCDVTPLL"
     gene            4137..5156
                     /gene="tilS"
                     /locus_tag="VFO88_02645"
     CDS             4137..5156
                     /gene="tilS"
                     /locus_tag="VFO88_02645"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET9544451.1"
                     /translation="MDTAAIRARVEEHVRRHDLIRPGGEVTCLVSGGADSTCLWHALS
                     ALGYRVAALHVNHGLRGAESGEDARFCRDVLGAEVVEAPADPASATEAELRDLRYAVA
                     RDRLRATGHTASDQVETILYRLATSGTTTGIRVHRDDGVVRPLLPLWREETEEYCRAE
                     GLAFRTDSSNDGTVRGLIRREIVPALRRLHPQAEQNVLAALEEPAALPQPVARAIAEL
                     LAAPAGSRRADLGGGRVAVREREGVWLERAPASLDGGPVIWGPWRIEASRPGLRVRGW
                     RAGDRLARRAKKVQDLFVDAKVPRSERDAWPLVVRGGEVVAVPGVATAPGWEDAVTAT
                     REEPA"
     gene            5153..5734
                     /gene="hpt"
                     /locus_tag="VFO88_02650"
     CDS             5153..5734
                     /gene="hpt"
                     /locus_tag="VFO88_02650"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET9544452.1"
                     /translation="MSEPRSSGTVQPSLEAGVEEILIEEDALQRRIAELGAQITEDYR
                     GRDLLLVCVLKGAVFFLSDLMRRIDLPVEVDFMAISSYGAGVDSSGVVRILKDLDVPI
                     EDRNVLIVEDIVDSGLTLSYLLRALESRGPATLEVCALLTKPERRENEVECRYIGFEI
                     PNRFVIGYGLDLAERYRNLPYIGVVRDELVAEA"
     gene            5806..>6696
                     /locus_tag="VFO88_02655"
     CDS             5806..>6696
                     /locus_tag="VFO88_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9544453.1"
                     /translation="MSRFFRSALFPLLIIILLVWLASQTLLPNQDERTELPYSALIET
                     VETNSDSVTELLIIPKEREIQATIDGEKTTSHYPSPEAIVPFQETLVANEIGFDSKGT
                     GENAWWSFFIYLIPFLLFFGFWIFLMNQMQGGGSKVMSFGKSRAKRMAPDSPKITFKD
                     VAGVDEAVEELHEIKEFLENPKKFQALGARIPKGVLLYGPPGTGKTLLARSVAGEAGV
                     PFFSISGSDFVEMFVGVGASRVRDLFEQAKQASPCIIFMDEIDAVGRHRGAGLGGGHD
                     EREQTLNQLLVEMDGFEMKDN"
BASE COUNT          940 a         2448 c         2420 g          888 t
ORIGIN      
        1 ccgcggagcg gcatgctcgt tctccgcgtg cccggcgagt cgtacgccgc cgcgatggac
       61 gcgctcggcc ggctcggcga gatcgaccac gagagcgagg aggggaccga cgtctcgcag
      121 gagtacgtcg acctcgaggc gcggcagcgt cacctcgagg cggtcgaggc gcagctcctc
      181 gagctgctcg agcgggcacg gacggtgcag gcggcgctcg ccgtgcagtc gcagctgaac
      241 ggcacgcagc tcgagctcga gcaggtgcgc ggccggctcc gcttcctcga cgaccgcacc
      301 gagttcgcca cgatcacgct cgagctccgg gagcccgagc gcgaggaggc ggacgcgggc
      361 ggctggggcg tcgtcgacgc ctggaaggcc ggcgcgcacg cgttcgtcac ggtcgcgcgc
      421 tgggcgttcg tgctcgtcgc ggccgtcgcg ccgctcctgc tcgtcgccgt cctcggctgg
      481 ctcggcgccc gcgccgtccg ccgccgccgc gccgccgctc cgacagggtg atcccgccgg
      541 gtcgaacccg ggtaaggtga tcggggtcga cagggaggca ggaatggcga agcaggagaa
      601 cgacaccggt gtcctcaacc ggctcgcggg ccgcggcgag gaggccgtga accggctcat
      661 ggaggagctc ggcaagaacg agaaggtcac ggacgcgctc gcgcgcgcga tggaggcgaa
      721 gggcaaggtc gacgagcgga cgcgcaagac gctcggcacc gtcgggctcg ccgccgccga
      781 ccagctcgag gggctccgca accagatcga ggcgctggag aagcggctcg cggcgctcga
      841 gaagacggag gccggcaagg ccggcgagac ggccgcgacc gcgcgctcga cgaccgcggc
      901 gaagccgaag acgacggcgg ccaggccgaa gccgaaggcc acgcccgaga cgccgaagac
      961 gaccgccgcg aggaagccgc cgaccacgcg caagagcccg ccgccgccga cggacgagcc
     1021 gaccggctcc ggctaacgag caggcacgcg gagcctgagg gctccgggca ggaggtcgac
     1081 ctcgagcggg gtcgtccccg gctgctcgcc gtcgagcgct atcgggagcg gctcctcgga
     1141 ctcgatccgg acggtgcggc cgcgcagcgt ctcgatcttc gggtgcctca cgtgggtgcc
     1201 gcggtagatc ttcgggaacg tgaggacgaa gtcgatcttc gtcacgtcgc cgatcgtgat
     1261 cacgtcgagg agcccgtcgt cgggcgccgc gccgggcgtg acgcgcatcc cgcccgcggc
     1321 gtactcgccg atcgtggcta gaacctcgaa catcgggccg gcgcggcgct cgccgtcgac
     1381 cgtgagctcg accggcgtcg ccttccagcc ggcgaacacg gcgatcgtcg cccacatgaa
     1441 ggagacgcgc ccgccggcgg ccttcgacgt cgcgttcgcg cgccgggcga tcgcgccgct
     1501 gatcccggcg ccggcgaagt tcgcgaagtt cgactcggcg ggcgtgccgt cccaggcgac
     1561 gtagcgcgcc cggccgacgt ccacggcgcg cacgcgtccc tcgcgcgccg tcgcgaacgc
     1621 gcggtcgagg tcgcccgtga tcccgaaggt gcggacgaag tccgtgccgg tgccgcgcgg
     1681 gacgatcgcc acgtcgacct ccgcggacgc gcccgagcgg atcaggccgt tcacggcctc
     1741 gtacaccgtc ccgtcgccgc cgacgaccac gatgaccgcc gcgccgtcgg cgaccgcgcg
     1801 ctcggcgagc tccgcgatct cgccctgccg cgtcgagatg agcgcctcgc cctcgagccc
     1861 cgccgcggcg gcccggtggg cgatctccgc ccagcggcgt ccggtcgagc cgttcgccga
     1921 cgccgggttc acgaggaaga cgacgccgtc gcgcgccacc gggatcagcg ctcggcggtc
     1981 acggtgccga gcgcgagctc ggtcatcccg tccacggcgg ccttcaggtc ctcgtcggag
     2041 atccgcttga actccccctc gacgatcacg tcgctgacgg tgaggaggca gcccgcctgc
     2101 accttgcgca gcgcgccgag cgtgaagagg acggccgcct ccatctcgac ggcgaggatc
     2161 ccgcggtcgg accagcgctg cgcgcgcccc tggtcggggt cgtagaagac gtcgctcgag
     2221 acgatcggcc cgacgcgaac gcgcttgccg agctccttcg ccgcgtgcgc cgcgccgtgg
     2281 acgagctccc agttcgcggt cggcgcgtgc ggctccccgc cgacgtagtg cgtcgcggtc
     2341 gagtcggccg gcacggcgga gagggcgatg atgaggtcac cgagctcgag gtccggctgg
     2401 aggcccccgg acgtgccgac gcggagcagc cgcttgacgc cgagctggac gagctcctcg
     2461 atcacgatcc ccgccgacgg gcagcccatc cccgtggact ggacgctgac cggccgcccc
     2521 tggaacgtcc cggtgaaccc gagcatccct cgctcggcgt tcgtctgccg cacgtcgtcg
     2581 aggaacgtct ccgcgatgta gcgggcccgc agcgggtcgc cggggaggag gcaggcctcc
     2641 gcgtagtcgc ccggctcggc tcgcacgtgg atcggcacgc gcgggagcct aacgcgcggg
     2701 cgagcccggc gcacgcgtcg gggatactcc cggcgtgcga cggctcgtac gccggccgga
     2761 ctcgaagcgg atcctcgcct tctgcgccga ggagccggtc gagcgggtct tcctcgagga
     2821 cgtcgcgcgc cgggggctcg gccgcttcgc cgcgctcgag gaggacgcgg gctacgtcgc
     2881 cgtctgccac atcggcgcga acgtcgtccc gtcaggcgcc gggacggccg cgttcgcctc
     2941 gctcgcggcc gaggccgagc cgcgcatgat cgtgggggag gagggagccg tgagcgagct
     3001 ctgggacgct gtgcgagtcg cgctcccgac gccgctcgac gaccgtccgg gacagcccgt
     3061 gtacgagctg agggagccgc cgccgccggg cgagagcggg ctccgagcgg cgacgttcga
     3121 cgatctcgag ctgctcgtcc ctgccgcggc ggccgcgtac ctcgaggagg tcgggatcga
     3181 cgcgtacgag cgcgacccgg ccctcttcga gtggcgcacg cgggtgcagg tcgagcaggg
     3241 gcggagctgg ctctggcgcc gcgacgggga gatcctcttc aaggccgagg cctccgcgtg
     3301 gaccgaccgg gccgtgcagc tccagcaggt ctgggtcgac ccgaggctgc gcggccatgg
     3361 ctacgcaaag cgcggcctcg ccgatctctg ccgcctgctg ctcgagcgga cgccgatcgt
     3421 ctgtctcttc gtccggccgg agaacacgcc cgcgctcgcg ctgtacgact ccctcgggat
     3481 gcgcaggacg atcgcgtacc ggtcgctcat cttcccgtag ccaggcctgg ttacaggctc
     3541 acccgggcga ggcatggcgc ccgctgcacg gcgccccgat gcttcgtctg cggtgcgtgg
     3601 aatcggagtg actgcggcgg ctctcgtcct cgccttcgga atcggcttcg ccatgcgcgg
     3661 cggaggcggc gaggcggcgt atgcgcacag ctgcagcgcg acggacaagc gtttcatcca
     3721 ggcggcctcg acggacatga ccgccctcgc cgtgtgggcc gacggctacc gcgccggcga
     3781 gctcgacgcc gacgaggtcg cccgccaggc gagcgacgcc gcgcggcgcg tcggctacgt
     3841 gaagccgcgc gacccgtcgc tcaagctctc gcagcggctc atcaacgcga tgttcgtgga
     3901 gtacggcgag gccgtctcgg tcggcaagaa ggagaagaag cgcgccggcc tccacatgca
     3961 ccgcgcctac gggctcgcga acttcgcccg tgaggtcctc gtgcaggcgc agccgggcct
     4021 cgcccgcgag ggctgcgacg tgaccccgct cctctgagcc gcgcgccttg taacactgtg
     4081 ctactagcgc ccggccgccc gccgtcggct agtgctgtcg cgcctacaat cgcggaatgg
     4141 acaccgccgc gatccgggcc cgcgtcgagg agcacgtccg ccgacacgac ctgatccggc
     4201 ccggcgggga ggtcacgtgc ctcgtctccg gcggcgccga ctcgacgtgc ctctggcacg
     4261 cgctctccgc gctcgggtac cgggtcgccg cgctccacgt gaaccacggg ctccgcggcg
     4321 ccgagtccgg ggaggacgcc cgcttctgcc gcgacgtcct cggcgccgag gtcgtcgagg
     4381 cgcccgccga cccggcgagc gcgaccgagg cggagctccg cgacctccgc tacgccgtcg
     4441 cgcgcgatcg cctccgcgcg accggccaca ccgcctccga ccaggtcgag acgatcctct
     4501 accgcctggc gacgagcggg acgacgacgg ggattcgcgt ccaccgcgac gacggcgtcg
     4561 tccgcccgct cctccccctc tggcgggagg agaccgagga gtactgccgt gccgaggggc
     4621 tcgcgttccg caccgactcc tcgaacgacg ggaccgtgcg cgggctcatc cggcgcgaga
     4681 tcgtcccggc gctgcgccgg ctccacccgc aggccgagca aaacgtgctc gccgcgctcg
     4741 aggagccggc ggcgctcccg cagcccgtcg cgcgcgcgat cgccgagctc ctcgccgcgc
     4801 ccgccggctc gcgccgcgcc gacctcggcg gcggccgcgt cgccgtccgc gagcgcgagg
     4861 gcgtctggct cgagcgagcc cccgcgtccc tcgacggcgg gccggtcatc tgggggccct
     4921 ggcggatcga ggcgagccgt cccggcctgc gcgtccgcgg ctggcgcgcg ggggatcggc
     4981 tcgcacgccg cgccaagaag gtgcaggatc tcttcgtgga cgcgaaggtc ccccgctcgg
     5041 agcgcgacgc ctggccgctc gtcgtgcgcg gcggcgaggt cgtcgcagtc cccggcgtcg
     5101 cgaccgcgcc gggctgggag gatgccgtca cggccacgcg agaggagccc gcatgagcga
     5161 gccgcgttcc agcggcaccg tccagccgag cctcgaggcc ggcgtcgagg agatcctgat
     5221 cgaggaggac gcgctccagc ggcgcatcgc cgagctcggc gcgcagatca cggaggacta
     5281 ccgcggccgc gacctcctgc tcgtctgcgt cctcaagggt gcggtcttct tcctctccga
     5341 cctcatgcgc cggatcgacc tgccggtcga ggtggacttc atggcgatct cgagctacgg
     5401 cgccggggtc gattcctcgg gcgtcgtccg gatcctcaag gacctcgacg tgccgatcga
     5461 ggaccggaac gtcctgatcg tcgaggacat cgtcgactcg ggcctcacgc tctcgtacct
     5521 cctgcgcgcg ctcgagtcgc gcggccccgc gacgctcgag gtctgcgcgc tcctcacgaa
     5581 gccggagcgg cgcgaaaacg aggtcgagtg ccgctacatc ggcttcgaga tcccgaaccg
     5641 cttcgtgatc ggatacgggc tcgacctcgc cgagcgctac cgcaacctcc cctacatcgg
     5701 ggtcgtgcgg gacgagctcg tcgccgaagc gtaggaggcc cggcaggcaa gccgggtgcc
     5761 gcgcagaacc ccgctgctac ccttcgatcc aggagacaac cccttttgag ccgtttcttt
     5821 cgcagcgcac tcttcccgct cctcatcatc atcctgctcg tctggctggc gtcgcagacg
     5881 ctcctgccga accaggacga gagaacggag ctgccctaca gcgccctgat cgagacggtc
     5941 gagaccaact ccgactcggt gaccgagctc ctgatcatcc cgaaggagcg cgagatccag
     6001 gcgacgatcg acggggagaa gacgacctcg cactacccga gcccggaggc gatcgtcccg
     6061 ttccaggaga cgctcgtcgc gaacgagatc ggcttcgact cgaagggcac gggtgagaac
     6121 gcctggtggt cgttcttcat ctacctgatc ccgttcctcc tcttcttcgg gttctggatc
     6181 ttcctcatga accagatgca gggcggcggc tcgaaggtga tgagcttcgg gaagtcgcgc
     6241 gcgaagcgga tggcgcccga ctcgccgaag atcacgttca aggacgtggc cggcgtggac
     6301 gaggcggtcg aggagctgca cgagatcaag gagttcctcg agaacccgaa gaagttccag
     6361 gcgctcggcg cgcggatccc gaagggcgtc ctcctctacg ggccgcccgg caccgggaag
     6421 acgctgctcg cgcgctcggt cgcgggcgag gcgggcgtgc cgttcttctc catctcgggc
     6481 tccgacttcg tcgagatgtt cgtcggcgtc ggcgcctcgc gcgtgcgcga cctcttcgag
     6541 caggcgaagc aggcgagccc ctgcatcatc ttcatggacg agatcgacgc cgtcggccgg
     6601 caccgcggcg cgggtctcgg aggcggccac gacgagcgcg agcagacgct gaaccagctc
     6661 ctcgtcgaga tggacggctt cgagatgaag gacaac
//
LOCUS       DASTFC010000089         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_248749,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000089 DASTFC010000000
VERSION     DASTFC010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248749"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFO88_02660"
     CDS             complement(<1..577)
                     /locus_tag="VFO88_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544454.1"
                     /translation="MRPNGRFLLFVAFAFTVMADPVSSIAYAIEAALAALDGDLASLV
                     PTMALVVGTIAIVAVTYHDLIRRFPGGGGGPEALGAAFGEGWAFVPLGALLVDFTLTI
                     AVSCAAAASAFIAYAPGLADGRVPIAAGLALVVGAACLFGHRGRLVFAGATIGFVGLT
                     VFVLVRGLGAEPVGDAAPIAGDASLLAVLLAM"
     gene            complement(586..1533)
                     /locus_tag="VFO88_02665"
     CDS             complement(586..1533)
                     /locus_tag="VFO88_02665"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9544455.1"
                     /translation="MRALVFHGPGDVRLEDVPEPEPGPGDVLVQVEVALTDGTDAKAY
                     RRGHPVLLGPPPSPFGHEFCGIDVATGRRVVAANSAPCGACPPCRRREETLCENLFPL
                     LNGAYAELLLVPERIARVNLLPVPHGLAPEVAALLEPLACCLHGIERAAIEPEQTVAV
                     VGVGPIGLMLCAAARDAGAHVVAVGGRASRRELAQEFGATPGDGKDADVVLEAAGTEE
                     AWRTAVELVRPGGTVVLFGGLPRDAAPPVDAYRLHYEELTLRGAFHHAPRHVRAALAF
                     LASGAAPWERLVTHRVGLGGVAALFDEPPDDLLKAAVVP"
     gene            1601..>2059
                     /locus_tag="VFO88_02670"
     CDS             1601..>2059
                     /locus_tag="VFO88_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544456.1"
                     /translation="MEREKPLRLDRRQLLVGGGLVAGAAGVGGAAAYALRRDGGPAPA
                     AQPAAAAADDDREFALDGDHTDLTTFVLAPHPRHVREAIERHRAALDASPSLALLEGE
                     PALEDAARAAAAAYLGTSPDLVALTDSTTMGLGLVSAGLGLAEGDEVLTTE"
BASE COUNT          279 a          762 c          817 g          201 t
ORIGIN      
        1 gcatcgcgag gagcacggcg aggagcgacg cgtcaccggc gatcggcgcc gcgtcgccga
       61 ccggctctgc gccgaggccg cggacgagca cgaacaccgt cagcccgacg aacccgatcg
      121 tcgcgccggc gaacacgagg cgcccgcgat gaccgaagag gcaggcggcg ccgacgacga
      181 gcgcgaggcc cgcggcaatc ggcacgcgac cgtccgcgag gcccggggcg taggcgatga
      241 aggccgaggc ggcggcagcg cagctcacgg cgatcgtgag cgtgaagtcg acgaggagcg
      301 cgccgagcgg cacgaacgcc cacccctcgc cgaaggccgc gccgagcgcc tccgggccgc
      361 cgccgcctcc cgggaagcgg cggatcaggt cgtggtacgt gacggcgacg atcgcgatcg
      421 tccccacgac gagggccatc gtcggcacga gcgacgcgag gtcgccgtcg agcgccgcga
      481 gcgccgcctc gatcgcgtag gcgatcgagg agactggatc ggccatcacc gtgaaggcga
      541 aggcgacgaa gaggaggaag cggccgttcg ggcgcatgcg cgctcctacg gaacgacggc
      601 cgccttgagg aggtcgtcgg gcggctcgtc gaagagggcg gccacgcccc cgaggccgac
      661 gcggtgcgtg actaggcgct cccacggcgc cgcgccgctc gcgaggaacg ccagggcggc
      721 ccggacgtgc cgcggcgcat ggtggaaggc accgcggagc gtcagctcct cgtagtgaag
      781 gcgatacgcg tcgaccggcg gcgccgcgtc gcgcgggagg ccgccgaaga ggacgaccgt
      841 gccgccgggc cgaacgagct cgacggcggt tcgccaggcc tcctccgtgc cggccgcctc
      901 gaggacgacg tcggcgtcct ttccgtcgcc cggggtcgcg ccgaactcct gcgcgagctc
      961 gcggcggctc gcccgcccgc cgacggcgac gacgtgcgcg ccggcgtcgc gcgcggccgc
     1021 gcacagcatg agcccgatcg ggccgacgcc gaccaccgcg accgtctgct ccggctcgat
     1081 cgccgcgcgc tcgatcccgt gcaggcagca ggcgagcggc tcgaggagcg ccgcgacctc
     1141 cggcgcgagc ccgtgcggga cggggaggag gttcacgcgc gcgatccgct ccgggacgag
     1201 gagcagctcc gcgtaggcgc cgttcaggag cgggaagagg ttctcgcaga gcgtctcctc
     1261 gcggcggcgg cagggcgggc acgcgccgca gggggccgag ttcgccgcga cgacgcgccg
     1321 cccggtcgcg acgtcgatcc cgcagaactc gtggccgaag gggctcggcg gcgggccgag
     1381 cagcaccggg tggccgcgcc ggtaggcctt ggcgtccgtc ccgtcggtga gggcgacctc
     1441 gacctggacg agcacgtcgc cgggcccggg ctccggctcc gggacgtcct ccagccggac
     1501 gtcgccgggg ccgtggaaga cgagcgcgcg cacgcccgga tcgtacggct gccgtgctcg
     1561 gcgccgtgga accccggcgg ccggactacc gtattgcgac atggagcggg agaagcccct
     1621 ccggctcgac cggcggcagc tgctcgtcgg cggcgggctc gtcgcgggcg cggcgggcgt
     1681 gggcggcgcc gcggcctatg cgctgcggcg cgacggcggg ccggcgccgg cggcgcagcc
     1741 tgccgcggcg gccgctgacg acgaccgcga gttcgcgctc gacggcgacc acacggatct
     1801 gacgacgttc gtgctcgcgc cccatccgcg ccacgtccgc gaggcgatcg agcgccaccg
     1861 cgccgcgctc gacgcgagcc cgtcgctggc gcttctcgag ggcgagccgg ccctcgagga
     1921 cgccgcccgt gcggcggcgg cggcctacct cgggacgagc cccgacctcg tcgcgctgac
     1981 cgactcgacc acgatgggcc tcggcctcgt ctccgcgggg ctcgggctcg ccgagggcga
     2041 cgaggtgctg acgacggag
//
LOCUS       DASTFC010000090         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1089458,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000090 DASTFC010000000
VERSION     DASTFC010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089458"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /gene="sufB"
                     /locus_tag="VFO88_02675"
     CDS             complement(<1..1052)
                     /gene="sufB"
                     /locus_tag="VFO88_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET9544457.1"
                     /translation="MAVTETEVVQGIGSDYEVKFGFSVPEDYFFKSGRGLSHELVDAI
                     SSHKDEPDWMRKFRHQSLDYFYARPMPKWGGDLSGIDFDNIYYYIRPTEKQADKWEDL
                     PADILDTWDKLGIPEAEKKYLAGVGAQYESEVVYHKLQADLEAQGVIFLDMDSGLREH
                     EELVKQYFGTVIPQNDNKFAALNSAVWSGGSFIYVPPGVSIEMPLQAYFRINAENMGQ
                     FERTLIIVDEGAYVHYVEGCTAPIYSSDSLHSAVVEIVVKKGGRCRYTTIQNWSNNVY
                     NLVTKRAVAYENATMEWVDGNLGSKLTMKYPAIWLMGERAHGEVLSIAFGGKGQHQDA
                     GGKVVHVAPRTSSVITS"
     gene            complement(1052..1837)
                     /gene="sufC"
                     /locus_tag="VFO88_02680"
     CDS             complement(1052..1837)
                     /gene="sufC"
                     /locus_tag="VFO88_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET9544458.1"
                     /translation="MALLEIKNLHVALEDGTEIVKGVDLAIDTNEVHAVMGPNGSGKS
                     TLAYALMGHPAYEITEGEILLDGEDLVELEADERAQRGLFLAFQYPYAVPGVTVASFL
                     RSAVNAVRKARNGGVDDPVPVPEFRKRLMAAMDELGVAKEMAGRYLNEGFSGGEKKRI
                     EILQMAMLDPRIAVLDETDSGLDIDALRVVAGGVKGLVGETMGALVITHYQRILNYIT
                     PDYVHVFVGGRIVAEGGPELAHKLEAEGYEAFAPAEALAGGES"
     gene            1994..2233
                     /locus_tag="VFO88_02685"
     CDS             1994..2233
                     /locus_tag="VFO88_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9544459.1"
                     /translation="MPGSIGWQGLVVILLILLLVFGPKRLPEMGRSLGRGMREFKDSI
                     TGNDVDEEPEHTELAPPIERDRTTAPKKPESERVP"
     gene            2243..3022
                     /gene="tatC"
                     /locus_tag="VFO88_02690"
     CDS             2243..3022
                     /gene="tatC"
                     /locus_tag="VFO88_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9544460.1"
                     /translation="MPRLPRRLQHGESATLVEHLEELRTRLIISLLAVVVFFVPTYIF
                     RRTLIDWLQGPVPEGFELTTLSPGEPFMTSFTVALYAAVALAVPVVVWQIWAFLAPAL
                     PEQSQLVVVRLVAAATVLLFCGMAFAYWVVLPNAIGFLLNFDSEIYATQLRAREYLSF
                     AALLLVGSGVMFQLPIFILGLVRLGILSSARLRRNRRIGYGIALLAVVFLPGVEFVSM
                     ALQAAPVMLLFEASIWLSIFFERRWEASGVLPEPRFGTGDS"
     gene            3019..4314
                     /locus_tag="VFO88_02695"
     CDS             3019..4314
                     /locus_tag="VFO88_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9544461.1"
                     /translation="MTRVLSADWVLPIEGPPIADGAVALEDGRIAAVGTRAELGEGER
                     FDGAAILPGLVNAHTHLEYAVYAGFGDGLPFGPWLALHIERKARIGWEEFLAIARLGA
                     AECLRSGVTTVADASYSGAAAVAMRDLGLRGIVGLEVFGSDPSRLAEVEERRRALEAE
                     TGGAVELAISPHAPYSVSADLYRGALATSRVAITHVAESDDELDYLARGDGPIARVSE
                     IEPPGTTPVRHLAAEGLLGPGVLAAHCVKVDEEEIALLAAHDVAVAHCPRSNALLGCG
                     IAPLAELRAAGLRVGLGTDSPASAPSFDMFEEARAAVYAARARAAAPDALSAGDALEL
                     ATLGSARALGLDGEIGSLAPGKRADLAVVSLAGSPYLPWEDPAAAVVFGGAPERVELT
                     LVAGEERYRKGTTAWHELRRRAESARRLLLGVLPPAPKT"
BASE COUNT          571 a         1535 c         1508 g          749 t
ORIGIN      
        1 gacgtgatca ccgatgacgt ccgcggtgcg acgtggacga ccttgccgcc cgcgtcctgg
       61 tgctggccct tgccgccgaa cgcgatcgag agcacctcac cgtgtgcgcg ctcgcccatg
      121 agccagatgg ccgggtactt catcgtgagc ttcgagccga ggttgccgtc cacccactcc
      181 atcgtcgcgt tctcgtaggc gaccgcacgc ttcgtgacga ggttgtagac gttgttcgac
      241 cagttctgga tcgtcgtgta ccggcagcgc ccgcccttct tcacgacgat ctcgacgacc
      301 gccgagtgca gcgagtcgga cgagtagatc ggcgccgtgc agccctcgac gtagtgcacg
      361 tacgcgccct cgtcgacgat gatcagagtg cgctcgaact ggcccatgtt ttccgcgttg
      421 atgcggaaat aggcctggag cggcatctcg atcgagacgc ccggcggcac gtagatgaag
      481 gagcctcccg accagacggc gctgttcagc gccgcgaact tgttgtcgtt ctgcgggatc
      541 acggtgccga agtactgctt gacgagctcc tcgtgctcgc gcaggcccga gtccatgtcg
      601 aggaaaatca cgccctgtgc ctccaggtcg gcctggagct tgtggtagac gacctcggac
      661 tcgtactggg cgccgacgcc ggcgaggtac ttcttctccg cctccgggat cccgagcttg
      721 tcccacgtgt cgaggatgtc ggccgggagg tcctcccact tgtcggcctg cttctcggtc
      781 gggcggatgt agtagtagat gttgtcgaag tcgatccccg acaggtcgcc gccccacttc
      841 ggcatcgggc gggcgtagaa gtagtcgagg ctctggtgcc ggaacttccg catccagtcc
      901 ggctcgtcct tgtggctcga gatcgcgtcg acgagctcgt gcgagagccc tcgtccggac
      961 ttgaagaagt agtcctccgg gacggagaac ccgaacttga cctcgtagtc ggagccgatt
     1021 ccctggacga cctcggtctc ggtgacggcc actacgactc accccctgcg agcgcttcgg
     1081 ccggcgcgaa cgcctcgtag ccctcggcct cgagcttgtg cgcgagctcc gggccgccct
     1141 cggcgacgat cctgccgccg acgaagacgt ggacgtagtc cggcgtgatg tagttgagga
     1201 tccgctggta gtgcgtgatc acgagcgccc ccatcgtctc gccgacgagg cccttgaccc
     1261 cgcccgctac gacgcgcagc gcgtcgatgt cgaggcccga gtcggtctcg tcgagcaccg
     1321 cgatccgcgg gtcgagcatc gccatctgga ggatctcgat ccgcttcttc tcgcccccgg
     1381 agaacccctc gttcagatag cggccggcca tctccttcgc gacgccgagc tcgtccatcg
     1441 cggccatgag cctcttgcgg aactccggca ccgggacggg gtcgtcgacg ccgccgttgc
     1501 gcgccttccg caccgcgttc accgcggagc ggaggaagct cgccacggtc acgccgggga
     1561 cggcgtacgg gtactggaac gcgaggaaga ggccgcgctg cgcgcgctcg tccgcctcga
     1621 gctccacgag gtcctcgccg tcgagcagga tctcgccctc ggtgatctcg taggccggat
     1681 ggcccatgag cgcgtaggcg agcgtgctct tgccggaccc gttcgggccc atgacggcgt
     1741 gcacctcgtt cgtgtcgatc gcgaggtcga cgcccttgac gatctcggtg ccgtcttcga
     1801 gggcgacgtg caggttcttg atctcgagca aagccaagtt cgtgacagcc cttcgcttga
     1861 agtttccagg gcgagccggg gaggctcgtg acgcacccag tgtagcggct cggccgtggc
     1921 ctcccgcggc cacgccgggc ccgtcgagtc gggcctccgt gtcgaaggag agccacggtg
     1981 ctatcctcgg tgcatgcctg gaagcatcgg ctggcagggc ctcgtcgtca tcctgctcat
     2041 ccttcttctc gtcttcggac cgaagcgact gccggagatg ggccgctcgc tcgggcgtgg
     2101 gatgcgcgag ttcaaggact cgatcaccgg caacgacgtc gacgaggagc ccgagcacac
     2161 cgagctcgcg ccgccgatcg agcgcgatcg gacgaccgcg cccaagaagc ccgagagcga
     2221 gcgcgtcccc taggcggtcg cggtgccgcg gcttccgcgt cgcctgcagc acggcgagag
     2281 cgcgacgctc gtcgagcacc tggaggagct gcggacgcgg ctcatcatct cgctgctcgc
     2341 ggtcgtcgtc ttcttcgtcc cgacgtacat cttccgcagg acgctgatcg actggctcca
     2401 ggggccggtg cccgaggggt tcgagctgac gacgctctcg ccgggcgagc cgttcatgac
     2461 ctcgttcacg gtcgccctct acgcggccgt cgcgctcgcc gtgcccgtcg tcgtgtggca
     2521 gatctgggcg tttctcgcgc cggccctgcc cgagcagagc cagctcgtcg tcgtccgcct
     2581 ggtcgcggcg gcgacggtgc tcctcttctg cgggatggcg ttcgcgtact gggtcgtcct
     2641 gccgaacgcg atcgggttcc tgctcaactt cgactcggag atctacgcga cgcagctgcg
     2701 ggcgcgcgag tacctgtcgt tcgcggcgct tctgctcgtc ggcagcggcg tgatgttcca
     2761 gctcccgatc ttcatcctgg ggctcgtgcg gctcggcatc ctctcgtcgg cgcgcctgcg
     2821 ccgcaaccgg cggatcggct acgggatcgc gctcctcgcc gtcgtcttcc tccccggcgt
     2881 cgagttcgtc tcgatggcgc tgcaggcggc gccggtgatg ctgctcttcg aggcctcgat
     2941 ctggctctcg atcttcttcg agcggcgctg ggaggcgtcc ggcgtcctgc ccgagccgcg
     3001 cttcggcacc ggcgactcgt gacccgcgtc ctctcggccg actgggtcct cccgatcgag
     3061 ggcccgccga tcgccgacgg cgcggtcgcg ctcgaggacg ggcggatcgc cgccgtcggc
     3121 acacgcgcgg agctcggcga gggcgagcgc ttcgacgggg cggcgatcct ccccgggctc
     3181 gtgaacgcgc acacgcacct cgagtacgcg gtctacgcgg gcttcgggga cgggctcccg
     3241 ttcgggccgt ggctcgccct ccacatcgag cgcaaggcgc ggatcgggtg ggaggagttc
     3301 ctcgcgatcg cgcggctcgg cgcggccgag tgcctgcgct ccggcgtgac gacggtcgcc
     3361 gacgcgagct actcgggtgc cgcggcggtc gccatgcgtg acctcggcct gcgggggatc
     3421 gtggggctcg aggtcttcgg ctcggacccg tcccggctcg cggaggtcga ggagcgccgg
     3481 cgcgcgctcg aggccgagac gggcggcgcg gtcgagctcg cgatctcccc gcacgcgccc
     3541 tactcggtgt cggccgacct ctaccgcggc gcgctcgcga ccagccgcgt cgcgatcacc
     3601 cacgtcgcgg agtcggacga cgagctcgac tacctcgccc gcggggacgg cccgatcgcg
     3661 cgcgtgagcg agatcgagcc gccggggacg acgccggtgc gccatctcgc cgccgagggg
     3721 ctgctcgggc ctggcgtcct cgccgcccac tgcgtgaagg tggacgagga ggagatcgcg
     3781 ctcctcgcgg cgcacgacgt cgcggtcgcg cactgtccgc gctcgaacgc gctcctcgga
     3841 tgcgggatcg cgccgctcgc cgagctccgc gcggccggcc tccgcgtcgg cctcggcacg
     3901 gacagcccgg cgtcggcgcc ctccttcgac atgttcgagg aggcccgcgc ggccgtgtac
     3961 gcggcccgcg cgcgcgccgc ggcgccggat gcgttgtcgg ccggcgacgc cctggaactc
     4021 gctacccttg gctcggcacg cgcgctcggt ctcgacggag agatcgggtc gcttgctccg
     4081 gggaagcgcg ccgacctcgc ggtcgtctcg ctcgccgggt ccccgtatct tccgtgggag
     4141 gaccccgcag cggcggtcgt cttcggagga gcgccggaac gagtagagct gaccctcgtc
     4201 gcaggcgagg agcggtacag gaaaggaacg acggcatggc acgagcttcg acgaagagca
     4261 gaaagcgctc gacgcctcct gctcggcgtc ctgcctccag cgccgaagac ctgatgttct
     4321 tcccgaagct tcgccgccgg gcgaagtggg tgttcgcgtt cct
//
LOCUS       DASTFC010000091         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_430026,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000091 DASTFC010000000
VERSION     DASTFC010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430026"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFO88_02700"
     CDS             complement(<1..758)
                     /locus_tag="VFO88_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9544462.1"
                     /translation="MARIEEDGDSGTAGAAGAAGAATDREERPRPAGDRPRVVTEESL
                     AELALGPFLDDLLARIRDALAADTVVILLAEPDQSELYAAAVLDFEEEISERVRVPVG
                     KGFAGRVAATGQPVVIEDLEHADAVNHLLRKRGLTSLLGVPLRLDDDVVGVLHVGSVA
                     PRVFGPADIEVLEGIGERIALAIGRARAHQAERRAHERLAFLAEVSHVLSSSLDHEET
                     LARLGRLSVPRLADSYVVMLVDGGRLVPAGIAHS"
     gene            complement(805..>2566)
                     /locus_tag="VFO88_02705"
     CDS             complement(805..>2566)
                     /locus_tag="VFO88_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET9544463.1"
                     /translation="CVISAGFAEHGEEGRRRQDELLALVRAHGARMVGPNCLGIALAD
                     PPLNATFAAGSFPPGNVGLCSQSGALGIALLERAAARGLGFSSFVSIGNKADVSTNDL
                     LEHWQDDDATAAVCFYVESFGNPHKFGRVARRVARRKPLLALKGGASRAGARAAASHT
                     AALAGSDAAVDALFRQAGVTRAATLEELLDAAALLSSQPLPRGPRVALLTNAGGLAIL
                     CADAAEAAGLEVGPLSPETEAELRSFLPAEAAAGNPVDVLGSSGADAYERALPLLLAD
                     PLVDSVIVLAVATVAFSPADLEPVLAELESDKPVLAVGTGAPGVADFGYPESAARALG
                     RAVERAAWLRRPLGTPPELDGIDETAGEAVVEAVLARGGGWLEPEEARALLGAYGIPF
                     VGERDAAGPDEAVAAARELGFPAVVKTAEAGAHKTETGGVALDLEDEEAVREAAERIG
                     GPVVVQPMAQGDVELLAGLLQDPVFGAVVAFGPGGVLAELAGGTSFALAPLTDVDAEE
                     LVRTGTAGRLVAGFRGAEPANAGALASVLLRLARLGEDRPEVAELDLNPVLAGPDGCV
                     AVDARVRVGPPVTRRGPKTW"
BASE COUNT          316 a          997 c          980 g          273 t
ORIGIN      
        1 tccgaatgcg cgatccccgc ggggacgagc cgcccgccgt cgacgagcat gacgacgtag
       61 ctgtcggcga ggcgcggcac ggagaggcgg ccgaggcgcg cgagcgtctc ctcgtggtcg
      121 agcgagctcg agaggacgtg gctcacctcg gcgaggaacg cgagccgctc gtgcgcgcgg
      181 cgctccgcct ggtgcgcgcg cgcccggccg atcgcgagcg cgatccgctc gccgatcccc
      241 tcgagcacct cgatgtcggc gggcccgaac acgcgcggcg cgaccgagcc gacgtggagg
      301 acgccgacca cgtcgtcgtc gaggcggagc ggcacgccga gaagggacgt gagcccgcgc
      361 ttgcggagga ggtggttcac ggcgtccgcg tgctcgaggt cctcgatcac gaccggctgc
      421 ccggtcgcgg cgacgcggcc ggcgaagccc ttgccgaccg ggacgcggac gcgctcggag
      481 atctcctcct cgaagtcgag gacggccgcg gcgtagagct cggactggtc cggctcggcg
      541 agcaggatga cgacggtgtc ggcggcgagc gcgtcgcgga tgcgggcgag gaggtcgtcg
      601 aggaacggcc cgagggcgag ctccgcgagc gactcctcgg tgacgacgcg cgggcggtcg
      661 ccggccggcc tcggccgctc ctcccggtcc gtcgcggcgc cggccgcgcc ggccgcgccg
      721 gcggtcccgc tgtccccgtc ctcctctatg cgcgccactg tgggcgccgg ttcccgttcg
      781 ggccaaacgc cattcgggac cgtgtcacca ggtcttcggg ccgcgacggg tgacgggggg
      841 cccgacgcgc acgcgcgcgt cgaccgcgac gcagccgtcc gggccggcga gcacggggtt
      901 gaggtcgagc tcggccacct ccggccggtc ctcgccgagc cgcgcgagcc ggagcaggac
      961 gctcgcgagg gcgcccgcgt ttgccggctc ggcgccgcgg aacccggcga cgagccgccc
     1021 ggccgttccc gtgcggacga gctcctccgc gtccacgtcc gtgagcgggg cgagcgcgaa
     1081 cgaggtcccg ccggcgagct cggccagcac gccgcccggc ccgaacgcga cgaccgcgcc
     1141 gaagaccgga tcctggagca gccccgcgag cagctcgacg tcgccctgcg ccatcggctg
     1201 cacgacgacc gggccgccga tccgctccgc cgcctcgcgc acggcctcct cgtcctcgag
     1261 gtcgagcgcg acgccgcccg tctcggtctt gtgcgcgccc gcctccgcgg tcttcaccac
     1321 cgccgggaag ccgagctcgc gggccgccgc gacggcctcg tccggcccgg ccgcgtcccg
     1381 ctcgccgacg aacgggatcc cgtaggcgcc gaggagcgcc cgggcctcct ccggctcgag
     1441 ccagccgccg cctcgcgcga ggacggcctc gaccacggcc tcgccggccg tctcgtcgat
     1501 cccgtcgagc tcgggcggcg tcccgagcgg ccggcggagc cacgcggccc gctccaccgc
     1561 gcgcccgagg gcccgcgcgg ccgactccgg gtagccgaag tccgcgacgc ccggcgcgcc
     1621 cgtcccgacc gcgagcacgg gcttgtcgga ctcgagctcg gcgagcaccg gctcgagatc
     1681 ggccggagag aacgcgaccg tcgcgacggc gaggacgatc accgagtcca cgagcgggtc
     1741 ggcgaggagc agcggcagcg cccgctcgta cgcgtccgcg ccggagctcc cgagcacgtc
     1801 gacggggttc ccggcggccg cttcggcggg gagaaacgag cggagctccg cctcggtctc
     1861 gggcgagagc ggccccactt cgagcccggc cgcctcggcc gcgtcggcgc agaggatcgc
     1921 gagcccgccc gcgttcgtga ggagcgcgac ccgcggcccg cgcggaagcg gctgcgagga
     1981 gaggagcgcg gccgcgtcga gcagctcctc gagcgtcgcc gcgcgcgtga cgccggcctg
     2041 gcggaacagc gcgtcgacgg ccgcgtcgga ccctgcgagc gccgccgtgt gcgaggcggc
     2101 cgcccgtgca cccgcgcgcg aggccccgcc cttgagcgcg aggagcggct tgcgccgcgc
     2161 gaccctccgg gcgacgcgcc cgaacttgtg cgggttgccg aacgactcga cgtagaagca
     2221 gaccgccgcg gtcgcgtcgt cgtcctgcca gtgctcgagc aggtcgttcg tcgagacgtc
     2281 cgccttgttc ccgatcgaga cgaacgacga gaacccgagt ccccgcgcgg ccgcccgttc
     2341 gaggagcgcg atcccgagcg cgccgctctg ggagcagagc ccgacgttcc cgggcgggaa
     2401 cgagccggcg gcgaacgtcg cgttgagcgg gggatcggcg agcgcgatcc cgaggcagtt
     2461 cgggccgacc atccgcgccc cgtgcgcgcg gacgagcgcg aggagctcgt cctgccggcg
     2521 ccgcccctcc tcgccgtgct ccgcgaaccc cgccgagatg acgcag
//
LOCUS       DASTFC010000092         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_990389,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000092 DASTFC010000000
VERSION     DASTFC010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990389"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1890)
                     /locus_tag="VFO88_02710"
     CDS             complement(49..1890)
                     /locus_tag="VFO88_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544464.1"
                     /translation="MELRRQQALELPAPLRSLLALAAAAVAFAAPPAALGQPGNVYDG
                     EGNLVETPFVPRDEVEPRLTEEEAIQAVLLRPKVADWVERYEGQELTQEGTFDTDTGL
                     WEVKVWAPGESGQIVEGKVEDASARVTEAWTGPQVAWRMARGYEGAFGRTINNPAVWL
                     GLCALFLLGLADLRRPLSLRNLDLLVLLSLSISLRFFNEGDVFTAMVLAYPPLLYLLG
                     RLVWIGTGRRRAPRAPMRPVWPVWLLLALTVFAAGFRIGLNTQTSNVIDVGYASVIGA
                     QRIVAQGEMPYGHMPTDEGEECGPADSDGYVRERIQTNGRCESANGRGDTYGPVTYLG
                     YVPGYLIFGWTGRWDSLPAAHFTAILFDLLALAGLALVGRRFGGNRLAVTLAFAWVAY
                     PFTQYASSSNSNDTIMPALLIWGFWLVSSPFGRGAFVALAAWSKFAALLLWPLWASYP
                     RGLARDAGPRLLRYGAGILVGTALAFSVLLLEPNVVDAARTFWDRTFGWQLSRPSPFS
                     VWDWGRYPGMPDLAWLQGALKIALVAGAAALYFVPRQKSPLQVAALSGALLIGFELVL
                     THWFYLYIVWFFPFAALALLAPALPRRAVPEEPAPDDRESRALVPAG"
     gene            complement(1845..2261)
                     /locus_tag="VFO88_02715"
     CDS             complement(1845..2261)
                     /locus_tag="VFO88_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET9544465.1"
                     /translation="MPTGRLHPALRRQANWVQLLKFCIVGGSGYIVNLAVYSALVVGL
                     DVHYAPAAVCSFLVAVTNNYTWNRAWTFRRQRGHLVHQGMRFLVVSTVALGANLVFLT
                     LLVAAGLPEIPAQAVAIVLVTPWNFVVNKLWSFRLR"
     gene            complement(2297..>3000)
                     /locus_tag="VFO88_02720"
     CDS             complement(2297..>3000)
                     /locus_tag="VFO88_02720"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET9544466.1"
                     /translation="LHGRGRPRARRRLSAVDYERLRERMVERQLARRGIRDERVLEAM
                     RRVPRERFVAPGDRGEAYDDVPLAIGAGQTISQPYMVALIAEAAAVRPGSKVLDVGTG
                     SGYAAAVFAELGADVHTIERIPELAEQARENLRAAGYGEVHVHVGDGSLGVPEEAPFD
                     AIAVAAAAPGLPPALYEQLVTGGRLVVPVGGPAGQRLEVVVRSPEGPAVVHSVECRFV
                     PLVGAGGFRGRGQGR"
BASE COUNT          465 a         1068 c         1134 g          333 t
ORIGIN      
        1 acgaagagcg cgacgccggc gagcgcggcg agcgcgacct gcgagcgctc agcccgcggg
       61 gacgagcgct cggctctctc ggtcatcggg cgcgggctcc tcggggacgg cgcgccgcgg
      121 gagcgcgggc gcgagcaggg cgagcgcggc gaaggggaag aaccagacga tgtagaggta
      181 gaaccagtgc gtcagcacga gctcgaagcc gatgaggagc gcgcccgaga gcgcggcgac
      241 ctggagcggg ctcttctgcc ggggcacgaa gtagagcgcg gccgcgccgg cgacgagcgc
      301 gatcttgagc gcgccctgga gccacgcgag gtccggcatg cccggatagc gcccccagtc
      361 ccagacggag aacggcgagg ggcgtgagag ctgccagccg aacgtccggt cccagaacgt
      421 gcgcgcggca tcgacgacgt tcggctcgag caggagcacg gagaaggcga gcgcggtccc
      481 gacgaggatc cccgcgccgt agcgcaggag ccgcgggccg gcgtcgcgcg ccaggccgcg
      541 cggataggag gcccagagcg gccagagaag cagcgccgcg aacttcgacc aggccgcgag
      601 cgcgacgaac gccccccgcc cgaacggcga cgagacgagc cagaagcccc agatgaggag
      661 cgccggcatg atcgtgtcgt tcgagttcga gctcgaggcg tactgcgtga acgggtacgc
      721 gacccacgcg aacgcgagcg tgaccgcgag ccggttcccg ccgaagcgcc gtcccacgag
      781 ggcgagcccg gcgagcgcga gcaggtcgaa gaggatcgcc gtgaagtgcg ccgccgggag
      841 cgagtcccag cgccccgtcc agccgaagat gagatagccc gggacgtagc ccaggtaggt
      901 gaccggcccg tacgtgtcgc cacggccgtt cgccgactcg cagcggccgt tcgtctggat
      961 ccgctcgcgg acgtagccgt cgctgtcggc gggcccgcac tcctcgccct cgtcggtcgg
     1021 catgtggccg tacggcatct cgccctgcgc gacgatccgc tgcgcgccga tcacgctcgc
     1081 gtacccgacg tcgatcacgt tcgacgtctg cgtgttcagc ccgatgcgga acccggccgc
     1141 gaacacggtc agcgcgagca ggagccacac gggccagacg ggtcgcatgg gcgcacgcgg
     1201 cgcgcggcgc cggcccgtcc cgatccagac gagccgcccg agcaggtaga ggagcggcgg
     1261 gtaggcgagc accatcgccg tgaagacgtc cccctcgttg aagaagcgga gcgagatgct
     1321 gagcgacagg aggacgagca ggtcgaggtt ccgaagcgag agcgggcggc gcaggtcggc
     1381 gagcccgagg aggaagagcg cgcagagccc gagccagacg gccgggttgt tgatcgtccg
     1441 cccgaacgcg ccctcgtacc cgcgggccat gcgccaggcg acctgtgggc ccgtccaggc
     1501 ctccgtgacc cgggcgctcg cgtcctcgac cttcccctcg acgatctggc ccgactcgcc
     1561 cggggcccag accttcacct cccagaggcc cgtgtcggtg tcgaacgtcc cctcctgcgt
     1621 cagctcctgg ccctcgtagc gctcgaccca gtcggccacc ttcggccgga ggagcacggc
     1681 ctggatcgcc tcctcctccg tcagccgcgg ctcgacctcg tcgcgcggga cgaacggcgt
     1741 ctcgacgagg ttgccctcgc cgtcgtagac gtttccaggc tgcccgagcg cggccggcgg
     1801 cgccgcgaac gcgacggcgg ccgcggcgag cgcgagcagg ctcctcagcg gagccggaag
     1861 ctccagagct tgttgacgac gaagttccac ggcgtgacga ggacgatggc gacggcctgc
     1921 gccgggatct ccggaagccc ggccgcgacg agcagggtga ggaagacgag gttcgcgccg
     1981 agcgcgacgg tcgagacgac gaggaacctc atcccctggt ggacgaggtg cccgcgctgc
     2041 ctgcggaacg tccaggcgcg gttccacgtg tagttgttcg tgacggcgac gaggaacgag
     2101 cagaccgccg cgggcgcgta gtggacgtcg agccccacga cgagcgccga gtagacggcg
     2161 aggttcacga tgtagcccga cccgccgacg atgcagaact tgaggagctg cacccagttg
     2221 gcctgccggc gcagggcggg gtgcaggcgt cccgtcggca tccgcggaag cgtagtcggg
     2281 tgggcgccgg gctctctcat cgaccctgcc cccggccgcg gaagcccccc gcgccgacga
     2341 gcggcacgaa gcggcactcg accgagtgga cgaccgcggg cccttcgggg ctccggacga
     2401 cgacctcgag ccgttgccca gcggggcccc cgacggggac tacgaggcgg ccgccggtca
     2461 cgagctgctc gtagagcgcg ggcggcaggc ccggcgcggc ggcggcgacg gcgatcgcgt
     2521 cgaagggcgc ctcctccggc acgccgagcg acccgtcgcc gacgtggacg tgcacctctc
     2581 cgtacccggc cgcgcggagg ttctcgcggg cctgctccgc gagctcggga atgcgctcga
     2641 tcgtgtggac gtcggcgccg agctcggcga agacggcggc cgcgtagccg gacccggtgc
     2701 cgacgtcgag gaccttgctc cccggccgga cggcggccgc ctcggcgatc agcgcgacca
     2761 tgtacggctg cgagatcgtc tggcccgcgc cgatcgcgag cgggacgtcg tcgtacgcct
     2821 cgccacggtc gcccggcgcg acgaagcgct cgcgcgggac gcggcgcatc gcctcgagga
     2881 cgcgctcgtc ccggatcccg cgccgggcga gctggcgctc gaccatccgc tcgcggagcc
     2941 gctcgtagtc cacggcgctc agacgccggc gggctcgagg gcggccgcgc ccatggaggc
//
LOCUS       DASTFC010000093         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_100460,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000093 DASTFC010000000
VERSION     DASTFC010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100460"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VFO88_02725"
     CDS             <1..1557
                     /locus_tag="VFO88_02725"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET9544467.1"
                     /translation="LLRPGFLHAARRSLELIAAETKEIAALVGTPHLDRDLFNACAVC
                     ADGRVKAVYRKRFLPNYGVFDEDRYFQPGRDLVLLRLGDTLVGPTICEDIWQPGPPAT
                     DLALAGAHVIANISASPFHLGKGREREEMLAQRARDNVAWVVFVNAIGGQDELLFDGH
                     SLVVDEEGEVVARGPAFEETLLVVDIDPATATGRRLSDARRRAQARERVDLPDPPVVE
                     LGKPRRQEATAPLALAPILPGLEEMRLALELALRDYLEKNGFREVVLGISGGIDSALT
                     AALAAEALGPDRVTAVSMPSRFTSKETRADARSVAENLGVRFLELPINGIVNAVRKTL
                     AEPFAGTEPGLAEENIQARARGLVLMALSNKFGSLLLATGNKSELSVGYATLYGDMAG
                     GFALLKDVYKTDVFRLARHLNERAGRELIPQTTIDRPPSAELRANQRDEDSLPPYEEL
                     DRVLEAYVEQDRSREELEAEYDAETVHRALALIDRAEYKRRQAPPGVKLRPKAFGRDR
                     RTPITNRWRG"
     gene            1557..1952
                     /locus_tag="VFO88_02730"
     CDS             1557..1952
                     /locus_tag="VFO88_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9544468.1"
                     /translation="MPKRFEYAASINREGALCAEDRDRLDPGETWTPEHLVLAALCRC
                     TLSSLRFHAERDGLRAAGTAAASGVVTKREEDGRYAFVELLCGMDVTVEPAPDGLRDL
                     LARAERDCFVGASLTAPVEYEWRVNGELV"
     gene            complement(1949..3730)
                     /locus_tag="VFO88_02735"
     CDS             complement(1949..3730)
                     /locus_tag="VFO88_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544469.1"
                     /translation="MARARRLLTAAVAAGLVAVTLPGIAAADCEPVLPVADVTTGMAA
                     TGWTVAQGRTPVPFGVEVLGVATDGLGPGRDLIVVEVASPEVDAAGGIWAGMSGSPVY
                     APGGELIGAVGYGFSFAGSKIGGVTPAQDMVNLLGLPSSRALARPVPKTVELDRGLAR
                     TVARAAGVTVAEASTLARLRVPLAVSGLDSVGLGLLTRNFAHRRADGLVPFVAGSTSA
                     SAPLGAPPAAGENFAAAIAYGNVSMNAMGTTTYVCGGQALAFGHPFLHGGPAHLGANA
                     ADAFAVVPDPVYGPFKFGQAAEPVGVVDQDRLAGVRASLGVLPETIPIETTVHVPERG
                     VVQTATTDLVANDQAGFLTFASMLSVFGTAFDAVGGGFDTAGRGTARVWWTFEGERAD
                     GTPWRLARGNRFASRSAAAVSASFNPGFMLDALIWLPEENVKVTSVKVDATIEKAVRV
                     YRVARVLMARGNRPLEARRAVAARPGAKYRFRVVLRQFEGGTRNLDFTFRLPKNLGRF
                     ASIGFLSNGAYWLDDCAGGFCFPRDEFFFSDAVTLDEWLDVFRSMPRDDELRIVLNRG
                     VGRGGGVLGGVRLDGVVVGGARVTLRR"
BASE COUNT          558 a         1482 c         1414 g          467 t
ORIGIN      
        1 ctgctccgcc ccggcttcct ccacgcggcg cgccgcagcc tcgagctgat cgccgcggag
       61 acgaaggaga tcgcggcgct cgtcggcacg ccgcacctcg accgcgacct cttcaacgcg
      121 tgcgccgtct gcgcggacgg ccgggtgaag gcggtgtacc gcaagcgctt cctcccgaac
      181 tacggcgtct tcgacgagga ccgctacttc cagccgggcc gcgacctcgt gctcctcagg
      241 ctcggcgaca cgctcgtcgg cccgacgatc tgcgaggaca tctggcagcc ggggccgccc
      301 gcgaccgacc tcgcactggc gggcgcgcac gtgatcgcga acatctccgc ctcgcccttc
      361 cacctcggaa aggggcgcga gcgcgaggag atgctcgccc agcgcgcgcg cgacaacgtc
      421 gcctgggtcg tcttcgtgaa tgcgatcggc ggccaggacg agctgctctt cgacggccac
      481 tcgctcgtcg tggacgagga gggcgaggtg gtcgcccgcg ggccggcctt cgaggagacg
      541 ctcctggtcg tggacatcga ccccgcgacc gcgaccggcc gccggctctc cgacgcgcgc
      601 cgccgggcgc aggcgcgcga gcgcgtcgac ctccccgacc cgcccgtcgt cgagctcggg
      661 aagccgcgcc ggcaggaggc gacggcgccg ctcgcgctcg ccccgatcct ccccggcctc
      721 gaggagatgc ggctcgcgct cgagctcgcg ctgcgcgact acctggagaa gaacggcttc
      781 cgcgaggtcg tcctcgggat ctccggtggg atcgactcgg cgctcaccgc cgcgctcgcc
      841 gccgaggcgc tcggccccga ccgcgtcacc gccgtctcga tgccgtcgcg gttcacgtcg
      901 aaggagacgc gcgcggacgc gcgcagcgtg gcggagaacc tcggcgtccg gttcctcgag
      961 ctgccgatca acgggatcgt gaacgccgtc cgcaagacgc tcgccgagcc gttcgcgggc
     1021 accgagccgg gcctcgcgga ggagaacatc caggcccgcg cgcgcgggct cgttctcatg
     1081 gcgctctcga acaagttcgg ctcgctcctc ctcgcgaccg ggaacaagag cgagctctcg
     1141 gtcggctacg cgacgctcta cggggacatg gccggcgggt tcgcgctcct gaaggacgtg
     1201 tacaagacgg acgtcttccg cctcgcgcgc cacctgaacg agcgcgccgg ccgcgagctc
     1261 atcccgcaga cgacgatcga ccgcccgccg agcgccgagc ttcgcgcgaa ccagcgcgac
     1321 gaggactcgc tgccgccata cgaggagctc gaccgcgtgc tcgaggcgta cgtcgagcag
     1381 gaccgctcgc gcgaggagct cgaggcggag tacgacgcgg agaccgtcca ccgggcgctc
     1441 gcgctcatcg accgggccga gtacaagcgg cgccaggcgc cccccggcgt gaagctccgc
     1501 cccaaggcgt tcggccgcga ccgccgcact ccgatcacga accgctggcg cggctgatgc
     1561 cgaagcgctt cgagtacgcg gcgtcgatca accgcgaggg cgcgctctgc gcggaggacc
     1621 gcgaccggct cgacccgggc gagacgtgga cgccggagca tctcgtgctc gccgcgctct
     1681 gccgctgcac gctctcgagc ctgcgcttcc acgccgagcg cgacgggctc cgcgccgcgg
     1741 gcaccgcggc cgcgagcggc gtcgtcacga agcgcgagga ggacggccgc tacgccttcg
     1801 tcgagctcct ctgcgggatg gacgtcaccg tcgagcccgc gccggacggg ctccgcgacc
     1861 tcctcgcccg cgccgagcgc gactgcttcg tcggcgcctc gctcaccgcg cccgtcgagt
     1921 acgagtggcg cgtgaacggc gagctcgtct agcgcctgag cgtcacgcgc gcgccgccga
     1981 cgacgacgcc gtcgagtcgc acccctccga gaacgccgcc gccgcgcccg acgcctcggt
     2041 tgaggacgat ccgcagctcg tcgtcgcgcg gcatcgagcg gaagacgtcg agccactcgt
     2101 cgagcgtcac ggcgtccgag aagaagaact cgtcgcgcgg gaagcagaag ccgccggcgc
     2161 agtcgtcgag ccagtacgcc ccgttcgaca ggaagccgat cgaggcgaac cgcccgaggt
     2221 tcttcggcag gcggaacgtg aagtcgaggt tgcgcgtccc gccctcgaac tgccgcagga
     2281 cgacgcggaa ccggtacttc gcgcccggcc gcgcggcgac cgcgcggcgc gcctcgagcg
     2341 gcctgttgcc gcgcgccatg aggacccgtg cgacccggta cacgcggacg gccttctcga
     2401 tcgtcgcgtc gaccttgacg ctcgtgacct tgacgttctc ctccggcagc cagatgagcg
     2461 cgtcgagcat gaagccgggg ttgaacgagg ccgagaccgc cgcggccgac cgcgacgcga
     2521 agcggttccc gcgcgcgagc ctccacggcg tgccgtccgc acgctcgccc tcgaacgtcc
     2581 accagacccg cgccgtcccg cgcccggcgg tgtcgaaccc gccgccgacc gcgtcgaacg
     2641 cggtcccgaa cacgctcagc atgctcgcaa acgtgaggaa ccccgcctgg tcgttcgcga
     2701 cgagatccgt ggtggccgtc tggacgaccc cgcgctcggg cacgtggacg gtcgtctcga
     2761 tcgggatcgt ctcgggcagg acgccgaggc tcgcccgcac gccggccagc cggtcctggt
     2821 cgacgacgcc gacgggctcc gccgcctggc cgaacttgaa ggggccgtag accgggtcgg
     2881 ggacgacggc gaaggcgtcg gccgcgtttg ccccgaggtg agccggcccg ccgtggagga
     2941 acggatgccc gaaggcgagc gcctgcccgc cgcagacgta cgtcgtcgtc cccatcgcgt
     3001 tcatgctcac gttcccgtac gcgatcgccg cggcgaagtt ctcgccggcc gccggcgggg
     3061 cgccgagcgg ggcgctcgcc gacgtcgagc cggcgacgaa cgggacgagc ccgtccgctc
     3121 ggcggtgcgc gaagttccgc gtgaggaggc cgagcccgac ggagtcgagg ccggagacgg
     3181 cgagcggcac gcgcaggcgg gcgagcgtcg aggcctccgc gacggtgacg ccggccgcgc
     3241 gcgccaccgt ccgggcgagc ccacggtcga gctcgaccgt cttcggcacc ggacgcgcga
     3301 gcgcgcgcga ggaggggagc ccgaggaggt tcaccatgtc ctgcgcgggc gtgacgccgc
     3361 cgatcttcga cccggcgaac gagaacccgt acccgacggc cccgatcagc tcgccgccgg
     3421 gcgcgtagac gggagagccg gacatgccgg cccagatccc acccgcggcg tcgacctcgg
     3481 ggctcgccac ctcgaccacg atgaggtcgc gccccgggcc gagcccgtcg gtcgcgacgc
     3541 cgagcacctc gacgccgaac gggaccggcg tccggccctg cgcgaccgtc cacccggtcg
     3601 cggccatccc ggtcgtcacg tcggcgaccg ggagcacggg ctcgcagtcg gctgctgcga
     3661 ttccggggag cgtgacggcg acgaggccgg cggcgaccgc ggcggtcagg agtcgacggg
     3721 cgcgggccat ggacgcccga tgatgaccga ttgccggacc gaaaacaatg ctgtcttttc
     3781 cggtcagcgc cggccggcga gctcggcgag cgcgcggaac cagcgcaggc gctcggcgta
     3841 cgggacggcc gcgttcgcgg gcgacgtgga cggcaggacg aagaggcgcg tgtcgccgag
     3901 cgtccgctcc tggaggccga g
//
LOCUS       DASTFC010000094         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1534416,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000094 DASTFC010000000
VERSION     DASTFC010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534416"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VFO88_02740"
     CDS             complement(<1..1583)
                     /locus_tag="VFO88_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9544470.1"
                     /translation="MASPKFFTRAARSEDGWSELSPHGRAWESLYRDRWAHDKVVRST
                     HGVNCTGSCSWKVHVKEGIVAWETQQTDYPSNGPDMPEYEPRGCPRGASFSWYLYSPL
                     RVKYPYVRAVLLDMWREALERTGDPVEAWAEVVEDPEKARRYKSQRGRGGFVRASWPE
                     VADLVAAAHVHTVRRYGPDRVAGFSPIPAMSMVSYAAGTRFLSLIGGVCLSFYDWYAD
                     LPPASPQIWGDQTDVPESADWWNASYVIVWGTNIPQTRTPDAHFLAEARYRGQKVVVV
                     SPDYAGHTKFADHWLAANPGTDGALALAMAHVIFKEFHAEGRVPYFDDYARRYTDMPF
                     LVTLRERDGAYVADRFLRASDLGETGENAEWKTACLDEATGEPVVPNGSIGFRWGEEG
                     KGRWNLELEGARPALTLLDRAEELVPVDLPRFDEPAETEGGTVHRRGVPALRVGGRLV
                     TTVFDLLAAQLGVRRGDLPGEWPAGYDDPAPYTPAWQEEITGVDAALVTRVAREFARN
                     AERSNGRSMIMMGGGTNHW"
     gene            complement(1576..2766)
                     /locus_tag="VFO88_02745"
     CDS             complement(1576..2766)
                     /locus_tag="VFO88_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544471.1"
                     /translation="MPEPPSGASRNLGLSTFAFALCFSAWGLMAPLARRFEDDLDLSS
                     TETAVLIAIPVVLGSLLRIPVGLLTDRLGGRIMFTAILLLSAPPSVALGFAESYWALV
                     GVGFVLGVAGSSFAVGVPFVARWYGRERQGFAVGVYGMGNIGTAVAAFSAPAIVDGLG
                     RPALGLFAGAILVAGAALFWLRAEDAPGERPRTRYGEVLGAGWRLWRLALFYFVTFGG
                     FVAMAVFLPKLLVDWFDLSLVDAGLRAAGFTLVATVARPIGGSLADRYGAYPVLVLAF
                     AGIAVDAGVLAVFSAEPRIVPITLACLTLAGFLGAGSGAVFKLVPAEFPTSAGAATGI
                     VGAAGGLGGFFPPLLMGVVNDAFGSYTLGFVGLLVFTAGCLALAVSLLRTAAPSDVRG
                     GAGG"
     gene            2864..3169
                     /locus_tag="VFO88_02750"
     CDS             2864..3169
                     /locus_tag="VFO88_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544472.1"
                     /translation="MPYGDWINELPTGFFLAVHLGAFALGAGFARLAFARELGLVGAG
                     FALYALAELVYLTYHFDWTVFLFAHTIAEVLNLAAFTLVFAGAVHQAVRHPAVEATR"
     gene            3166..3492
                     /locus_tag="VFO88_02755"
     CDS             3166..3492
                     /locus_tag="VFO88_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9544473.1"
                     /translation="MRALAALPLVLVLAACGGTAQASEPVSTTEVAMAESYVFEPETI
                     EVEAGATVTWRNEDRFTHTVQVEGQEDHEVEPGASVSITFSEPGTYAYVCTLHSKDME
                     GEVIVR"
BASE COUNT          557 a         1301 c         1341 g          435 t
ORIGIN      
        1 taccagtggt tcgtcccgcc gcccatcatg atcatcgagc ggccgttcga gcgctcggcg
       61 ttgcgggcga actcgcgcgc gacccgcgtg acgagggccg cgtcgacgcc tgtgatctcc
      121 tcctgccacg cgggcgtgta cggggcggga tcgtcgtagc cggccggcca ctcgcccggc
      181 aggtcgccgc ggcgcacgcc gagctgcgcc gcgagcaggt cgaacacggt cgtcacgagc
      241 cgcccgccga cgcgcagcgc ggggacgccg cgccggtgga cggtgccgcc ctccgtctcg
      301 gccggctcgt cgaagcgcgg caggtcgacg gggacgagct cctccgcgcg gtcgaggagc
      361 gtgagcgccg gccgcgcgcc ctccagctcg aggttccagc ggcccttgcc ctcctcgccc
      421 cagcggaagc cgatcgagcc gttcgggacg acgggctcgc cggtcgcctc gtcgaggcag
      481 gcggtcttcc actcggcgtt ctcgcccgtc tccccgaggt cggacgcgcg caggaaccgg
      541 tcggccacgt acgccccgtc gcgctcgcgc agcgtgacga ggaacggcat gtcggtgtag
      601 cggcgcgcgt agtcgtcgaa gtacgggacg cggccctccg catggaactc cttgaagatc
      661 acgtgcgcca tcgcgagcgc gagcgcgccg tccgtgcccg ggttcgcggc gagccagtgg
      721 tcggcgaact tcgtgtgccc cgcgtagtcg ggcgagacga ccacgacctt ctggccgcgg
      781 tagcgcgcct cggcgaggaa gtgggcgtcg ggcgtccgcg tctgcgggat gttcgtcccc
      841 cagacgatca cgtagctcgc gttccaccag tcggccgact cgggcacgtc cgtctggtcg
      901 ccccagatct gcggcgacgc gggcgggagg tcggcgtacc agtcgtagaa cgagaggcag
      961 accccgccga tcagcgagag gaagcgcgtc cccgccgcgt acgagaccat cgacatggcc
     1021 gggatcggtg agaagccggc cacgcggtcc ggcccgtagc ggcgaacggt gtgcacgtgc
     1081 gcggcggcga cgaggtcggc cacctccggc caggacgcgc gcacgaagcc cccgcgcccg
     1141 cgctgcgact tgtagcggcg cgccttctcg gggtcctcga cgacctcggc ccacgcctcg
     1201 acgggatcgc cggtgcgctc gagcgcctcc cgccacatgt cgaggaggac ggcgcgcacg
     1261 tacgggtact tcacgcgcag cggcgagtag aggtaccagc tgaacgaggc gccgcggggg
     1321 cagccgcgcg gctcgtactc gggcatgtcc gggccgttcg agggatagtc ggtctgctgc
     1381 gtctcccacg cgacgatccc ctccttcacg tgcaccttcc acgagcacga gccggtgcag
     1441 ttcacgccgt gggtcgagcg gacgaccttg tcgtgcgccc agcggtcgcg gtagagcgac
     1501 tcccaggcgc ggccgtgcgg ggagagctcg ctccagccgt cctccgagcg cgccgcgcgc
     1561 gtgaagaatt tcgggctagc caccggcacc tccgcgcaca tccgaggggg cggcggtccg
     1621 gagcagcgac acggccaggg cgaggcagcc ggcggtgaac acgagcagcc cgacgaaccc
     1681 taacgtgtag ctcccgaacg cgtcgttcac gacgcccatg aggagcgggg gaaagaaccc
     1741 gccgagcccg cccgccgcgc cgacgatccc ggtcgccgcg ccggcgctcg tcgggaactc
     1801 ggccgggacg agcttgaaca ccgcgccgct cccggcgccg aggaagcccg cgagcgtgag
     1861 gcacgcgagc gtgatcggca cgatgcgcgg ctccgcggag aacacggcga ggactcccgc
     1921 gtccaccgcg atcccggcga aggcgagcac gaggaccggg taggcgccgt agcggtcggc
     1981 gagcgagccg ccgatcgggc gcgcgacggt ggcgacgagc gtgaacccgg ccgcgcggag
     2041 gccggcgtcg acgagcgaga ggtcgaacca gtcgacgagc agcttgggca ggaagacggc
     2101 catcgccacg aacccgccga aggtgacgaa gtagaagagc gcgagccgcc agagccgcca
     2161 gcccgccccg agcacctcgc cgtagcgggt gcggggccgc tcgcccggcg cgtcctccgc
     2221 ccgcagccag aagagcgcgg cgccggcgac gaggatcgcc cccgcgaaga ggccgagcgc
     2281 cggccggccg agcccgtcca cgattgcggg ggcgctgaac gccgcgacgg cggtgccgat
     2341 gttccccatc ccgtagacgc cgaccgcgaa gccctgccgc tcgcgcccgt accagcgggc
     2401 gacgaacggg acgccgaccg cgaacgacga gcccgcgacc ccgaggacga agccgacccc
     2461 gacgagcgcc cagtagctct cggcgaaccc gagcgcgacg ctcggcggcg cggacaggag
     2521 caggatcgcc gtgaacatga tcctcccgcc gaggcggtcg gtgagcaggc cgaccgggat
     2581 gcgcaggagc gagccgagga ccacggggat cgcgatcagg acggccgtct cggtgctcga
     2641 aaggtcgagg tcgtcctcga agcgccgcgc gagcggagcc atgaggcccc aggcgctgaa
     2701 gcagagcgcg aacgcgaagg tcgagaggcc gaggttccgg gaggcgccgg acggaggctc
     2761 tggcatcgct ccgtcgatcc tacgacgctc cgaccgagaa cgtaaggacc ggcggcgcgg
     2821 gcgcaccaat ccctccgaga ccagcgatca aggagggaac ggaatgccct acggcgactg
     2881 gatcaacgag cttcccaccg ggttcttcct cgcggtgcac ctgggcgcgt tcgcgctcgg
     2941 cgcggggttc gcccgcctcg cgttcgcgcg cgagctcggg ctcgtcggcg ccggcttcgc
     3001 gctctacgcg ctcgccgagc tcgtgtatct cacgtaccac ttcgactgga cggtcttcct
     3061 cttcgcgcac acgatcgcgg aggtcctgaa cctcgccgcc ttcacgctcg tcttcgcggg
     3121 ggccgtgcac caggcggtgc ggcatccggc cgtcgaggcg acgcggtgag ggcgctcgcc
     3181 gcactgccgc tcgtactcgt gctcgccgcc tgcgggggga cggcgcaggc gtccgagccg
     3241 gtctcgacga ccgaggtcgc gatggccgag agctacgtgt tcgagccgga gacgatcgag
     3301 gtcgaggccg gcgcgacggt cacgtggcgc aacgaggacc gcttcacgca caccgtccag
     3361 gtcgagggac aggaagacca cgaggtcgag cccggcgcga gcgtctcgat cacgttctcc
     3421 gagccgggca cgtacgcgta cgtctgcacg ctccacagca aggacatgga aggggaggtg
     3481 atcgtccgat gaccagcagg aagcgcacac gcatcacacg gctcgttgcg gcgctggtcg
     3541 cggttctcgc cgcggcgctc gtggcgggcg tgctcggagg cggccaggaa gcggtcgcgc
     3601 agggcaaggc tcaggcgaag cgaccggtct ccca
//
LOCUS       DASTFC010000095         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_842104,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000095 DASTFC010000000
VERSION     DASTFC010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842104"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFO88_02760"
     CDS             <1..349
                     /locus_tag="VFO88_02760"
                     /inference="COORDINATES: protein motif:HMM:NF018399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HET9544474.1"
                     /translation="AEAFGKGALVGLDGDVEHGSAIIHNLLFGNPVRELVGGTSLLPS
                     AEKRGAAGAPLDLALKHKEDHSVRSHHQTFEVRIPDAPRPDEIVVWVAFAATGRPHAR
                     LPAFGSELAADAS"
     gene            353..1225
                     /locus_tag="VFO88_02765"
     CDS             353..1225
                     /locus_tag="VFO88_02765"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9544475.1"
                     /translation="MARVAFIGVGTMGLPMARSLLAAGHDVVPVDADRERAALVGAPA
                     ATPAEAAAAADVALLSLPTPAAVEAVVLGPAGVLEGAVRGFAVVDTSTSPPALARRLE
                     AELAPAGIEFLDAPVSGGPRGAEDATLTVMVGGSREGFERMRPLFEALGSLVVHVGAA
                     GAGQAAKLCNNLIAGATMTAVAEACAIAEREGIEAATLYDLLTRSTGDSRVLRTRFPL
                     AGVTESHPASRGFAPLFALDLMAKDLELVLELAAARGVDAPVGSASLARYREAQRRGL
                     GALDYSAVFSARRP"
     gene            1222..>2363
                     /locus_tag="VFO88_02770"
     CDS             1222..>2363
                     /locus_tag="VFO88_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544476.1"
                     /translation="MSSSPRAVLTASSAAQASVSVVAFGLPAIGPDLREAFGLSLPML
                     GAALGATIFGSGLFLIGAGYLVDRHGARPSMLAGTALGSAGLAAAGFAPGPEALIALL
                     FVSGAGSAVVPIAGTRALFRAYEVDRRGWALGVRQMAVPLGGTVAALLLPGLVALGGI
                     RAALIGSAVLVAAAGLVFAGVAGAVEPVRERVPFALPRLWRTPGMQRLLLVAALYIVV
                     LQAVVAYMVPAARAAGFSPLVAGAAFVLVNVVAGVARIVWGRLADQGGGGRRRVRTLV
                     EAGGVAAAGAVVFSFALAAGGPVGLLAATVVLAFGALGWNALVYVTAGERVPRELAGQ
                     AVALAATVVFVVAALCTPALGALAELAGWNAFWFTAAVLALAGAAVA"
BASE COUNT          207 a          916 c          888 g          352 t
ORIGIN      
        1 ggccgaggcc ttcggcaagg gcgcgctcgt cggcctcgac ggcgacgtcg agcacgggtc
       61 ggcgatcatc cacaacctcc tcttcggcaa cccggtgcgc gagctcgtcg gcggcacgtc
      121 gctcctcccc tccgcggaga agcggggcgc cgccggggcg ccgctcgacc tcgccctcaa
      181 gcacaaggaa gaccattcgg tcaggtcgca ccaccagacc ttcgaggtcc gcatccccga
      241 cgcgccgcgg cccgacgaga tcgtcgtctg ggtcgcgttc gcggccacgg gtcgaccgca
      301 tgcacggctg ccggccttcg gcagcgagct cgccgccgac gcgtcctgac gcatggcccg
      361 cgtcgccttc atcggcgtcg ggacgatggg gctgccgatg gcccgcagcc tcctggcggc
      421 cggccacgac gtcgtccccg tcgacgccga ccgggagcgc gccgcactcg tgggcgcgcc
      481 cgccgcgacg cctgccgagg cggcagcggc cgccgacgtc gctcttctct ccctgcccac
      541 ccccgccgcc gtcgaggcgg tcgttctcgg cccggcgggc gtgcttgagg gagcggtgcg
      601 gggcttcgcc gtcgtggaca cgtccacgtc tccgccggct ctcgcccgga ggctcgaggc
      661 agagctcgcg ccggccggga tcgagttcct cgacgcgccc gtcagcggcg ggccgcgcgg
      721 cgccgaggac gcgacgctca cggtcatggt gggcggcagc cgggaggggt tcgagcggat
      781 gcgccctctc ttcgaggcac tcggcagcct cgtcgtccac gtcggtgcgg cgggcgccgg
      841 gcaggcggcg aagctctgca acaatctgat cgccggagcg acgatgaccg ccgtcgcgga
      901 ggcctgcgcg atcgccgagc gggaggggat cgaggccgcg acgctctatg acctgctcac
      961 gcgctccacc ggcgactcgc gcgtcctgcg gacgcgcttc cccctcgcgg gcgtgacgga
     1021 gagccatccg gcctcgcgag ggttcgcccc gctcttcgcg ctcgacctga tggccaagga
     1081 tctcgagctg gtgctcgagc tcgcggccgc ccgcggcgtc gacgcacccg tcgggtcggc
     1141 gagcctcgcg cgctaccgcg aggcgcagcg ccgggggctc ggagcgctcg actactccgc
     1201 cgtcttctcg gcgcgccggc cgtgagctcg tccccgcggg ccgtgctcac ggcgtcgtcc
     1261 gcggcgcagg cgagcgtctc ggtcgtcgcg ttcgggctgc ccgcgatcgg gccggacctc
     1321 cgcgaggcgt tcgggctctc gctgccgatg ctgggcgccg cgctcggggc caccatcttc
     1381 ggttcggggc tcttcctcat cggagcgggg tacctggtcg accgccacgg cgcgcgtccc
     1441 tcgatgctcg caggcaccgc gctcggctcg gccgggcttg ccgcggccgg cttcgcgccc
     1501 ggtcccgagg cgctcatcgc gctcctcttc gtgtccggtg ccggctcggc ggtcgtcccg
     1561 atcgccggca cgcgcgccct cttccgggcc tacgaggtcg accggcgggg gtgggcgctc
     1621 ggcgtccgcc agatggccgt gccgctgggc ggaaccgtgg cggcgctcct tctgccgggg
     1681 ctcgtcgccc tgggcgggat cagggccgct ctcatcggga gcgcggtgct cgtcgcggcc
     1741 gccggcctgg tcttcgcagg ggtcgccggt gccgtcgagc ccgtgcgcga gcgggtgccg
     1801 ttcgccctcc cccggctctg gcggacgccc ggcatgcagc ggctgctcct ggtcgccgcg
     1861 ctctacatcg tcgtcctgca ggccgtcgtc gcgtacatgg tccccgcggc ccgggcggcg
     1921 ggcttctcgc ccctcgtggc aggggccgcc ttcgtcctcg tgaacgtcgt cgcgggggtc
     1981 gcccggatcg tgtggggccg gctcgccgat cagggcggcg gcggtcgccg ccgcgtgcgc
     2041 acgctcgtcg aggcgggcgg cgtggccgcg gcgggggcgg tcgtcttctc cttcgcgctc
     2101 gcggccggcg gcccggtcgg cctgctcgcg gcaaccgtcg tgctcgcatt cggcgcgctc
     2161 ggctggaacg cgctcgtcta cgtgacggcg ggggagaggg tgccccggga gctggccggg
     2221 caggccgtcg cgctcgccgc caccgtcgtg ttcgtcgtcg ccgcgctctg cacgccggcc
     2281 ctcggcgccc tggccgagct cgccggctgg aacgccttct ggttcaccgc ggccgtcctc
     2341 gccctcgccg gggctgcggt cgc
//
LOCUS       DASTFC010000096         9975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_941031,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000096 DASTFC010000000
VERSION     DASTFC010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941031"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFO88_02775"
     CDS             <1..183
                     /locus_tag="VFO88_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9544477.1"
                     /translation="LAREAGAVVSRERILAEVWNTTWYGSAKTIDVHVASLRRKLGDP
                     SWIETVRGVGLRLRDA"
     gene            180..1556
                     /locus_tag="VFO88_02780"
     CDS             180..1556
                     /locus_tag="VFO88_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544478.1"
                     /translation="MTNRLLLGFLAVTVVVLAVLEIPLGITYARNERQDLTLKVERDA
                     VTLATLAADVLEPDGETRLGALERLADGYAVETGGRVVVVNARGRAVVDTSPEFGEGR
                     DFSTRPEMALALGGEVATGVRDSETLGQTLLYVAVPAVSGGSVLGAVRITYPTSAVDE
                     RVRRYWLILLAIAGVVVAAAAVAGLLLARWITSPLRAVERVAAAVGRGELDARAPAAG
                     PPEVRDLARVFNETAAKLQTLLHSQEQFVADASHQLRTPLTALRLRLENLEADTGPAS
                     QESLAGALAEVERLSALVEGLLALARSDASDAQAEPIDLAEIAATRAEAWSALADERG
                     VRLETASAGETVARAAPDRVEQVLDNLLSNALEVSPPGSTIRLAAARDGSRVELHVVD
                     EGPGLTPEQRERAFDRFWRGGSGEGGSGLGLAIVARLVAADGGDVELRAAVPRGIDAV
                     VRLPAAHA"
     gene            1611..2117
                     /locus_tag="VFO88_02785"
     CDS             1611..2117
                     /locus_tag="VFO88_02785"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HET9544479.1"
                     /translation="MSEITSPRDLFLHELGDILYVEQKLAEEVLPTLMEEVTDDELRK
                     GFEKHLRETEKHVENLEKAFQKLGEPAEAEECIGFEGLKSEHDQLVQESSPDLIDLVD
                     AGAAARTEHYEIAAYESLIAMARGLGERDVIELFEKNLKDEKETLREVESVAKRLAKE
                     HAKAGQTA"
     gene            complement(2124..3071)
                     /locus_tag="VFO88_02790"
     CDS             complement(2124..3071)
                     /locus_tag="VFO88_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HET9544480.1"
                     /translation="MRYRKLGSSDLVVSEIALGSWLTYGVGVETERSRACLERAFELG
                     INFIDTANVYGRGRAEEFLGAELAGRDRSSYVLATKLFFPMSDTDRGLSREQVLKQID
                     ASLARLRTDYVDLYQCHRYDRETPLEETMEALTEVVRSGKARYIGFSDWNAEQIQAAL
                     ELPGVEKFVSSQPQYSLLWRRPEREIFDLCRRNGISQIVYSPLAQGVLTGKYRPGERP
                     GTDTRAASDEMGWAMGKFLSDRVLEGVERLRPIADGLGVTLAQLSLAWILREENVASA
                     IVGASRPEQVEANASASGIELDDATLEAVDEALADATLS"
     gene            3556..4857
                     /locus_tag="VFO88_02795"
     CDS             3556..4857
                     /locus_tag="VFO88_02795"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9544481.1"
                     /translation="MPRQKSTHVDDPKAVGERLRTAREAAGLSQRQLSFTGCSPAYIS
                     RIEAGDRIPSLQLLRELGRRLGVTEDFLATGSEVRPQAINLLEAEIALRLDDVERARE
                     LYTQSLENARTPSERAQAGEGLGHLALRVGDARQAVALFEQALVDSRMEEWQVPTLAE
                     SLARAYSVVGELEPSVAILERCLAAFEQIGDRVQAIRFACVLGYALSDAGQFARAEQV
                     VSKALAEGRDSFDPYMRARVYWARAKLQLDQGDFDGASRYAYQALAALELTEDLHYTG
                     LAHQLVARIELERGEAQEALSHLAQGWPLVERTGTPIEQAQFRLEEARALAQTGDREQ
                     ATAIAMDVLGLLGDAEPLDGGRLYALLAEVALDLGDEDEALELYEKAAALLESAAGNR
                     ALVDVYAKLGEIYERLGRTDEAYAYMKKAVSTQQTLSVPRK"
     gene            4971..7541
                     /locus_tag="VFO88_02800"
     CDS             4971..7541
                     /locus_tag="VFO88_02800"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET9544482.1"
                     /translation="MQEAFPYPTDQSERVGGLAASALRAQSWAGEPFLQEDENPADVL
                     AAGRARRRALDDALAEMEAGLRAPSPEWKVRYALMLGLERVVNDRPPRLASGTELRRH
                     QIDALAGMLTELISKHEEGKENGNGNGHHEPEDEELPEEPGPDDDEDEDEEPELDDPG
                     AERRFRFRHPTASGKTIAAAGFVEGARTMGVLILTHRRLLVKQFERDLTVEGYGDRFR
                     DAIEQGLEPLVGNPITIQTYAWFARHVGSISRNAYQLVICDEAHTALGEKTSAAIRSF
                     PEPIYVGMTATEQLIAKQVSDVFPASVDDLPLGDAARRGLIAPLRSLRVPPATAISSV
                     PIVGGDYDQEALAKVLDHALINQAAASLYRDRFDATPGIVYAAGVEHAYNLAREFRAA
                     GLKAEAVSGRTPPLQLAETLAAYERGEINVLINAQLLAEGWNSPRATVCMHLAPTASR
                     RVYQQRIGRVMRTHPRKEAGVVVDFVDPAATHNDRTITIHSLLDSDFYRPGARVTPAP
                     RRRVQRRARRKLSPAPWLIPVTPDPRRRVLVIQREWERIDPGRLAEDEQLLWARIAGR
                     QLRFDERQQFAEKLAKASRECREAFLTSCAAENPNRRLRLSALGDRVTTPVDRASFDD
                     LVSMVTSAPTWEKDRVNGIRTLLRAVGEGKADAPQDIVSRWTWRLARATRKLQDRRAS
                     AELPDAKRLLGALTNSRGHRHEENAARLVNVALEQPIHVGAALLASAEAYTPTAQRLV
                     DGARERLGTIEEVANALADNLPQAKQPSGKSRRRRRRKKRQAEAAKAGKNGEGQAQDS
                     GEPQPGTDTKAPAAEDGTPPRKSGRRRSRRKPAAKKDAPAAEAEAPKPAEPQPAAEAS
                     "
     gene            complement(7538..8419)
                     /locus_tag="VFO88_02805"
     CDS             complement(7538..8419)
                     /locus_tag="VFO88_02805"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HET9544483.1"
                     /translation="MGEHLLMDERPSLERPVLVAAFRGWNDGAQGASLAVGYLAQAWA
                     AERFAEIDPEEFYDFQVARPHVALEDGVTRRIDWPENAFFHAAVPGGGRDVVLLLGVE
                     PNNRWRTFAAQIVDLAQELGVELVVTLGALLADVPHTRPAPVTGSATDPALVEELGLA
                     ASRYEGPTGIVGVLHDACRVAGVPSASLWAAVPHYASLAASPKAALALCRRFADLVSA
                     DVELHELEQAAETYERQVSEAVAENEETQAYVEGLERRRDALGDELDLPSGDTLAAEL
                     TRFLREHERRREEGEEA"
     gene            complement(8429..9346)
                     /locus_tag="VFO88_02810"
     CDS             complement(8429..9346)
                     /locus_tag="VFO88_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9544484.1"
                     /translation="MTEAAAERRRGLPRLAFDARAFRWAAWLALVALFAVTVSGATVR
                     LTGSGLGCENWPRCGDTFLPAQSYHALIEFGNRVVGFVVGLTTLLAALVAWRARVPRR
                     LLLWTLALPLLVLAQGILGGITVLFELHPLIVMGHFLLSLVSIGVAVVVVAGAERLHR
                     PRPSPALALPGWLGTLALALLPLAAVLVVTGAFVTAAGPHSGGEDIERLGDLENAMWV
                     HVRVSAVFGVAFLALVAALQRFRDRARGTALLAGGVLALLLVQMVVGEVQWRNQLPWE
                     LVLGHVTLATLVWTGVVALAVVLRRPQDA"
     gene            complement(9343..>9975)
                     /gene="selD"
                     /locus_tag="VFO88_02815"
     CDS             complement(9343..>9975)
                     /gene="selD"
                     /locus_tag="VFO88_02815"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET9544485.1"
                     /translation="LAGGHTIRDDEPKYGLAVVGTVHPGALWRKDGARPGDALFLTKP
                     LGTGLVLTASRKGVVGDAEHEAATASMTELNAAAADALRPFEPHAVTDVTGFGLFGHA
                     HEVAERSGVRLVLDAGALPALPGAVEAAEAGVRTGGDGRNRDYAGPALDLDGLPEPLA
                     ALGYDPQTSGGLLVSLPAERAALLAAEFSARGLFLARLGRVEEGSGVAVR"
BASE COUNT         1453 a         3699 c         3643 g         1180 t
ORIGIN      
        1 ctcgcgcgcg aggcgggcgc ggtcgtgagc cgcgagcgga tcctcgccga ggtctggaac
       61 acgacctggt acggatcggc gaagacgatc gacgtccacg tcgcgtcgct tcgccgcaag
      121 ctcggcgacc cgagctggat cgagaccgtg cgcggcgtcg gactccgcct gcgcgacgcg
      181 tgacgaaccg gctgctcctc ggcttcctcg ccgtcaccgt ggtcgtcctc gccgtgctcg
      241 agatcccgct cgggatcacg tacgcacgca acgagcggca ggacctgaca ctcaaggtcg
      301 agcgcgacgc ggtcacgctc gcgacgctcg ccgcggacgt cctcgagccg gacggcgaga
      361 cgcggctcgg tgccctcgag cggctcgcgg acggctacgc ggtggagacc ggcgggcgcg
      421 tcgtcgtcgt gaacgcccgc ggccgggcgg tcgtcgacac gtcgccggag ttcggcgagg
      481 gccgcgactt ctccacccgg ccggagatgg cgctcgccct cggcggggag gtcgccaccg
      541 gcgtccgcga ctcggagacg ctcgggcaga cgctgctcta cgtggccgtg ccggccgtgt
      601 cgggcgggtc cgtcctcggc gccgtgcgga tcacgtatcc gacctcggcc gtcgacgagc
      661 gggtccgccg gtactggctc atccttctcg cgatcgccgg ggtggtcgtc gccgcagccg
      721 cggtcgccgg gctgctcctg gcccgctgga tcacgagccc gctccgggcg gtcgagcgcg
      781 tcgccgccgc ggtcggccgg ggcgagctcg acgcccgcgc gccggccgcc gggccgcccg
      841 aggtgcgcga cctcgcccgc gtcttcaacg agacggcggc gaagctccag accctcctcc
      901 actcgcagga gcagttcgtc gcggacgcgt cgcaccagct ccggacgccg ctgacggcgc
      961 tccgcctccg gctcgagaac ctcgaggcgg acaccgggcc cgcgtcgcag gagtcgctcg
     1021 cgggagcgct cgcagaggtc gaacggctct ccgcgctcgt cgagggcctc ctcgcgctcg
     1081 cccgctcgga cgcgagcgac gcgcaggccg agcccatcga cctcgcggag atcgccgcca
     1141 cgcgagcgga ggcgtggtcg gcgctcgcag acgagcgcgg cgtccggctc gagaccgcct
     1201 cggccggcga gaccgtcgcc cgcgcggcgc ccgaccgggt ggagcaggtg ctcgacaacc
     1261 tcctgtcgaa cgcgctcgag gtgagcccgc cgggctcgac gatccggctc gccgcggcgc
     1321 gcgacgggag ccgcgtcgag ctccacgtcg tcgacgaggg gccgggcctc acgcccgagc
     1381 agcgcgagcg cgcgttcgac cgtttctggc ggggcgggtc gggcgagggc gggtccgggc
     1441 tcggcctcgc gatcgtcgcg cgcctcgtcg ccgcggacgg cggagacgtc gagctccggg
     1501 ccgccgtccc gcgcgggatc gacgcggtcg tgcgcctccc cgccgcgcat gcgtgagcac
     1561 gcgcggcggc gggtactcgc cgcttcggcc acgaaaggaa ggagacctgc atgtccgaga
     1621 tcaccagccc gcgcgacctg ttcctgcacg agctcgggga catcctctac gtcgagcaga
     1681 agctcgccga ggaggtcctc ccgaccctca tggaggaggt cacggacgac gagctccgca
     1741 agggcttcga gaagcacctg cgcgagaccg agaagcacgt cgagaacctc gagaaggcgt
     1801 tccagaagct cggcgagccg gccgaggcgg aggagtgcat cggcttcgag ggcctgaaga
     1861 gcgagcacga ccagctcgtc caggagtcct cgccggacct catcgacctc gtggacgccg
     1921 gcgctgccgc gcgcacagag cactacgaga tcgccgcgta cgagagcctc atcgccatgg
     1981 cacgcggcct cggcgagcgc gacgtgatcg agctcttcga gaagaacctg aaggacgaga
     2041 aggagacgct ccgcgaggtc gagtccgtcg cgaagcggct cgcgaaggag cacgcaaagg
     2101 ccgggcagac cgcctagccg aagtcagctc agggtggcgt cggcgagcgc ctcgtccacg
     2161 gcttcgagcg tcgcgtcgtc gagctcgatg ccggaggccg aggcgttcgc ctccacctgc
     2221 tccggccgcg acgcgcccac gatcgccgaa gcgacgttct cctcccggag gatccacgca
     2281 agcgagagct gggcgagcgt gacacccagg ccgtcggcga tcgggcgcag ccgctcgacg
     2341 ccctcgagca cccggtccga gaggaacttg cccatcgccc atcccatctc gtccgaggcg
     2401 gcgcgcgtgt cggtgcccgg ccgctcgccc ggccggtact tcccggtcag cacgccctgc
     2461 gcgagcgggg agtagacgat ctgcgagatg ccgttccggc ggcagaggtc gaagatctcc
     2521 cgctcgggcc ggcgccagag gagcgagtac tgcggctggc tcgagacgaa cttctcgacc
     2581 cccggcagct cgagcgccgc ctggatctgc tctgcgttcc agtcgctgaa gccgatgtag
     2641 cgcgccttcc cggagcggac gacctcggtg agcgcctcca tcgtctcctc gagcggcgtc
     2701 tcgcggtcgt agcggtggca ctggtagaga tcgacgtagt ccgtccgcag gcgggcgagc
     2761 gaggcgtcga tctgcttcag cacctgctcg cgcgagaggc ccctgtccgt gtcggacatg
     2821 gggaagaaga gcttcgtcgc gagcacgtac gacgagcggt cgcggccggc gagctcggcg
     2881 ccgaggaact cctccgccct cccgcgcccg tagacgttcg cggtgtcgat gaagttgatc
     2941 ccgagctcga acgcccgctc gaggcaggcg cgcgagcgct cggtctcgac gcccacgccg
     3001 tacgtgagcc acgagccgag cgcgatctcc gagacgacga ggtcgctcga gccgagcttc
     3061 cggtagcgca ttccggcgtt ctacccgatc cggccgagcg cgcgtccggg caggcgggga
     3121 gcacccgtta gggggacggc gctgcggtca ggttcgctta cttaagtcac gacgcgacga
     3181 gtcccgccgc atgccagtcc cgagggcgtc ccaccctcga gcgatcgcgg agaatcgtcc
     3241 cgccatcccg tccggcgaag gcccgccccc gcaggcgggc cttcgcgcgc ctggactcct
     3301 ccgtcgacga gagttcgcgc ctacacttcc cgtcaggcgc gaaccgtgtc aaactgaacg
     3361 aaacgactgt gctctacggg ccccagttgg gtccgacgaa ccccgctgcg gcggcgaacg
     3421 aagccaatgt ggccgaaagg atgagaagac gagccttcac ctggacaccg tcccttctgg
     3481 tggtgggcaa gaaacctgct aggccggacc ggcggcgtcg agtataactc ggccgaaaca
     3541 aggaaggaac cgaacatgcc ccgacagaag tccacgcacg tagacgatcc gaaggccgtc
     3601 ggcgagcggc tgcgcaccgc gcgcgaggcc gccggcctga gccagcggca gctgtcgttc
     3661 accggctgct cgcccgcgta catctcgcgg atcgaggccg gggaccggat cccgtcgctc
     3721 cagctcctgc gtgagctcgg tcggcggctg ggcgtgacgg aggactttct ggcgacgggg
     3781 agcgaggtgc gcccgcaggc gatcaacctg ctcgaggccg agatcgcgct gcgcctggac
     3841 gacgtcgagc gcgcgcgcga gctctacacc cagtcgctcg agaacgcccg gacgccgtcg
     3901 gagcgcgcgc aggccggcga ggggctcggc cacctcgcgc tccgggtcgg ggacgcccgg
     3961 caggcggtcg cgctcttcga gcaggcgctc gtggactcgc ggatggagga gtggcaggtg
     4021 ccgacgctcg ccgagagcct cgcgcgcgcc tactccgtcg tcggcgagct cgagccgtcc
     4081 gtcgcgatcc tcgagcggtg cctcgcggcg ttcgagcaga tcggggaccg ggtgcaggcg
     4141 atccggttcg cgtgcgtcct cgggtacgcc ctctccgacg ccggccagtt cgcccgcgcg
     4201 gagcaggtcg tctcgaaggc cctcgccgag ggccgcgaca gcttcgatcc ctacatgcgc
     4261 gcgcgcgtct actgggcccg cgccaagctc cagctcgacc agggcgactt cgacggcgcc
     4321 tcgcggtacg cctaccaggc gctcgcggcg cttgagctga cggaggatct ccactacacc
     4381 ggtctcgcgc accagctcgt cgcccgcatc gagctcgagc gcggggaggc gcaggaggcc
     4441 ctctcgcacc tcgcccaggg ctggccgctc gtcgagcgga cgggcacgcc gatcgagcag
     4501 gcgcagttcc gcctcgagga ggcgcgtgcc ctcgcgcaga cgggcgaccg ggagcaggcg
     4561 accgcgatcg cgatggacgt cctcgggctc ctcggcgacg ccgagccgct cgacggcggg
     4621 cggctgtacg cgctgctcgc cgaggtcgcc ctcgacctcg gcgacgagga cgaggcgctc
     4681 gagctctacg agaaggcggc cgcgctcctc gagtcggcgg ccggcaacag ggcgctcgtc
     4741 gacgtgtacg cgaagctcgg cgagatctac gagcgcctgg gtcgcacgga cgaggcgtac
     4801 gcgtacatga agaaggccgt ctcgacccag cagacgctct cggtgccgcg caagtaacga
     4861 gcccgccccg gcaatccggg ggccgtctcg tatatagtcc ccggtagtcc ggcgctggga
     4921 gttgcgccgg catctccctg aacatgcagc gcgttccgag cgctgcgtcc atgcaagagg
     4981 cattcccgta cccgaccgac cagtcggagc gagtgggcgg cctggcggct tccgcgctcc
     5041 gcgcccagtc ctgggcgggc gagcccttcc tccaggagga cgagaacccc gccgacgtgc
     5101 tggcggccgg ccgtgcccgg cggcgtgcgc tcgacgacgc gctcgccgag atggaggcgg
     5161 gcctgcgcgc cccgtcgccc gagtggaagg tgcgctacgc gctcatgctc gggctcgagc
     5221 gcgtcgtcaa cgaccgcccg ccgcggctcg cgtcggggac cgagctccgc cgccaccaga
     5281 tcgacgcgct ggccgggatg ctcaccgagc tcatctcgaa gcacgaggag ggcaaggaga
     5341 acgggaacgg caacgggcac cacgagcccg aggacgagga gctgcccgag gagccggggc
     5401 cggacgacga cgaggacgag gacgaggagc ccgagctcga cgaccccggc gccgagcggc
     5461 gctttcgctt ccgccacccg acggcgtcgg ggaagacgat cgccgccgcc ggcttcgtcg
     5521 aaggcgcgag gacgatgggc gtcctcatcc tcacgcaccg ccggctgctg gtgaagcagt
     5581 tcgagcgcga cctgaccgtc gaggggtacg gcgaccggtt ccgcgatgcg atcgagcagg
     5641 ggctcgagcc gctcgtgggg aacccgatca cgatccagac ctacgcctgg ttcgcccgcc
     5701 acgtcggctc gatctcccgc aacgcgtacc agctcgtcat ctgcgacgag gcgcacaccg
     5761 cgctcggcga gaagacgagc gccgcgatcc ggtccttccc cgagccgatc tacgtcggca
     5821 tgacggcgac cgagcagctc atcgcgaagc aggtctcgga cgtcttcccc gcctcggtcg
     5881 acgaccttcc gctcggcgac gccgccaggc gcgggctgat cgccccgctg cgctcgctcc
     5941 gcgtgccgcc ggcgacggcg atctcgtccg tcccgatcgt gggcggcgac tacgaccagg
     6001 aagccctggc caaggtcctc gaccacgcgc tcatcaacca ggcggccgcg agcctctacc
     6061 gcgaccgctt cgacgcgacg ccggggatcg tctacgcggc cggcgtcgag cacgcctaca
     6121 acctcgcgcg cgagttccgc gccgccggcc tcaaggcgga ggcggtgagc ggccgcacgc
     6181 cgcccctcca gctcgccgag acgctcgcgg cctacgagcg cggcgagatc aacgtcctca
     6241 tcaacgcgca gctcctcgcc gagggctgga actcgccccg cgcgaccgtc tgcatgcacc
     6301 tcgcgccgac cgcctcgcgg cgcgtctacc agcagcggat cggccgcgtc atgcggacgc
     6361 atccgcgcaa ggaggcgggc gtcgtcgtcg acttcgtcga cccggccgcg acgcacaacg
     6421 accggacgat cacgatccac tcgctcctcg actcggactt ctaccgtccg ggcgcgcgcg
     6481 tcacgcccgc gccgcgccgc cgggtgcagc gccgggcccg ccgcaagctc tcgccggcgc
     6541 cctggctcat ccccgtcacc cccgatccgc gccgccgcgt gctcgtcatc cagcgcgagt
     6601 gggagcggat cgatccgggc cgcctcgccg aggacgagca gctcctctgg gcgcggatcg
     6661 ccggacggca gctccgcttc gacgagcgcc agcagttcgc ggagaagctc gcgaaggcga
     6721 gccgggagtg ccgcgaggcg ttcctcacga gctgcgcggc cgagaacccg aaccggcgcc
     6781 tgcggctctc ggcgctcggc gaccgggtga cgacgcccgt cgaccgggcg agcttcgacg
     6841 acctcgtctc gatggtcacc tccgcgccga cgtgggagaa ggaccgggtg aacgggatcc
     6901 gcacgctcct ccgcgcggtc ggtgagggca aggcggacgc gccgcaggac atcgtctcgc
     6961 gctggacgtg gcgcctcgcc cgcgcgacgc gcaagctgca ggaccggcgc gcgagcgcgg
     7021 agctcccgga cgcgaagcgg cttctcggcg cgctcacgaa ctcgcgcggc caccgtcacg
     7081 aggagaacgc cgcgcgcctc gtgaacgtcg cgctcgagca gccgatccac gtcggcgcgg
     7141 ccctgctcgc gtcggccgag gcctacacgc cgaccgcgca gcggctcgtc gacggcgcgc
     7201 gcgagcggct cggcacgatc gaggaggtcg cgaacgcgct cgcggacaac cttccgcagg
     7261 cgaagcagcc gagcggaaag tcgcgccgcc gccggcgccg caagaagcgc caggccgagg
     7321 cggcgaaggc cggcaagaac ggcgaggggc aggcgcagga cagcggcgag ccgcagcccg
     7381 ggactgacac gaaggcgcct gcggccgagg acggcacgcc gccgcgcaag tcgggccggc
     7441 ggcgctcgcg caggaagccg gccgcgaaga aggacgcgcc ggcggccgag gccgaggcgc
     7501 cgaagccggc cgagccgcag cccgcggccg aagcctccta ggcctcctcc ccttcctcgc
     7561 ggcgccgctc gtgctcgcgg aggaagcgcg tgagctcggc cgcgagcgtg tcgccggacg
     7621 gcaggtcgag ctcgtcgccg agcgcgtcgc gccgccgctc gagcccctcc acgtacgcct
     7681 gggtctcctc gttctcggcg accgcctcgc tcacctggcg ctcgtacgtc tcggcggcct
     7741 gctcgagctc gtggagctcg acgtcggcgg agacgaggtc ggcgaagcgg cggcagagcg
     7801 caagcgccgc cttcgggctc gcggcgagcg acgcgtagtg cggcaccgcc gcccacaggc
     7861 tcgccgaggg gacgcccgcg acgcggcacg cgtcgtgcag gacgccgacg atcccggtcg
     7921 gcccctcgta gcgcgacgcc gcgagcccga gctcctcgac gagcgcggga tcggttgcgg
     7981 agccggtcac gggcgcgggg cgcgtgtgcg ggacgtcggc gaggagcgcc ccgagggtca
     8041 cgacgagttc gacgccgagc tcctgcgcca ggtcgacgat ctgcgccgcg aacgtccgcc
     8101 agcggttgtt cggctcgacc cccagcagga gcacgacgtc gcggccgccg cccggaaccg
     8161 ccgcgtggaa gaacgcgttc tccggccagt cgattcggcg cgtgaccccg tcctcgagcg
     8221 cgacgtgcgg gcgcgcgacc tggaagtcgt agaactcctc ggggtcgatc tccgcgaagc
     8281 gctcggccgc ccaggcctgc gcgaggtagc cgaccgcgag cgaggcgccc tgcgccccgt
     8341 cgttccagcc gcgaaacgcg gcgacgagca ccgggcgctc gagcgagggg cgctcgtcca
     8401 tcaggaggtg ctcgcccatg gacagagtct aggcgtcctg cggccgccgc agcacgaccg
     8461 cgagggcgac cacgcccgtc cagacgagcg tcgccagcgt gacgtggccg agcacgagct
     8521 cccacgggag ctggttccgc cactgcacct cgccgacgac catctggacg aggagcagcg
     8581 cgagcacgcc gccggcgaga agcgccgtcc cgcgcgcgcg gtcgcggaac cgctggaggg
     8641 cggcgacgag cgcgaggaac gccactccga agacggcgct cacccggacg tgcacccaca
     8701 tcgcgttctc gaggtcgccg agccgctcga tgtcctctcc gcccgagtgg ggcccggcgg
     8761 cggtgacgaa cgccccggtc acgacgagca ccgcggcgag cgggagcagc gccagcgcga
     8821 gcgttcccag ccagcccggg agcgcgagcg ccggggacgg gcgcggccgg tggagccgct
     8881 ccgcgcccgc gacgacgacg accgcgacgc cgatcgagac gagcgagagg aggaagtgcc
     8941 ccatcacgat cagcgggtgg agctcgaaga gcaccgtgat cccgccgagg atcccctgcg
     9001 cgaggacgag gagcggcagc gcgagcgtcc agaggagcag ccggcgcggc acgcgcgccc
     9061 gccaggcgac gagcgccgcg aggagcgtcg tgagcccgac gacgaacccg acgacgcggt
     9121 tcccgaactc gatcagcgcg tggtagctct gcgcggggag gaacgtgtcg ccgcagcgcg
     9181 gccagttctc gcagccgagc ccggagccgg tcaggcggac ggtcgcgccg ctcacggtca
     9241 cggcgaagag cgcgaccagc gcgagccacg cggcccagcg aaaggcgcgc gcgtcgaagg
     9301 cgagccgcgg cagcccgcga cgccgctcgg cggccgcctc cgtcaccgga cggcgacccc
     9361 gcttccctcc tcgacgcggc cgagacgggc gaggaagagc ccgcgcgccg agaactccgc
     9421 cgcgaggagc gcggcccgct cggccgggag ggagacgagc agcccgccgg aggtctgcgg
     9481 gtcgtagccc agcgccgcga gcggctccgg cagcccgtcg aggtcgagcg ccggcccggc
     9541 gtagtcgcgg ttccggccgt cgccgcccgt ccggacgccc gcctccgccg cttccacggc
     9601 gcccgggagt gccgggaggg cgccggcgtc gaggacgagc cggacgccgc tccgctccgc
     9661 gacctcgtgc gcgtgcccga agaggccgaa gcccgtcacg tcggtgaccg cgtgcggctc
     9721 gaacgggcgc agcgcgtcgg cggccgccgc gttcagctcg gtcatggagg cggtcgcggc
     9781 ttcgtgctcg gcgtccccga ccacgccctt acgcgaggcc gtgagcacga gccccgtgcc
     9841 gagcggcttg gtcaggaaga gcgcgtcgcc gggacgggcc ccgtccttgc gccagagcgc
     9901 gcccgggtgg acggtgccga cgaccgcgag cccgtacttc ggctcgtcgt cgcggatcgt
     9961 gtgcccgccg gcgag
//
LOCUS       DASTFC010000097         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_941034,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000097 DASTFC010000000
VERSION     DASTFC010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941034"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFO88_02820"
     CDS             complement(<1..185)
                     /locus_tag="VFO88_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544486.1"
                     /translation="MEVRSRADAEPFTTADGSTIRVLVDAEAGGARNQSLAEATLAPG
                     QATERHYHAETEELYVVL"
     gene            complement(188..1687)
                     /gene="gcvPB"
                     /locus_tag="VFO88_02825"
     CDS             complement(188..1687)
                     /gene="gcvPB"
                     /locus_tag="VFO88_02825"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9544487.1"
                     /translation="MQLVYEKSQPGRRAATLPDPGLPVPELPEALRREQAPRLPELSE
                     PELVRHFTALADRTFGVDTGFYPLGSCTMKHNPRVNERVVALPGFRDLHPLQEEDGAQ
                     GALELMWRLQEILAEVVGLDAVSLQPAAGSQGELTGLMLMRAYFEDRGEGEGRDTVFV
                     ADTAHGTNPASVTMAGYKLQKVGTDERGNLDVDFLREHVDERTAGLMLTNPSTLGLFD
                     ERIEEVADIFHGAGALLYYDGANLNAVCGISRPGDMGFDIVHYNLHKTFSQPHGGGGP
                     GGGPVAVRAQLEPFLPVPVVVRDGERFRFDEDRPKTIGKVRGYYGPFGVFVRSYAFIR
                     SYGPRLREMSEVAVLNANYLLARLRDAYDLPYDRLCMHEFVLSARTLKREHGVTALDV
                     AKRLMDYGFHPPTIYFPLIVPEALMIEPTETEPKERLDAFADAMLAIAREAAEEPGLV
                     KDAPHRRPVKRLDEVKAAKQAIVRYRFDEHPDAGAAREPAQPVEAPKGV"
     gene            complement(1708..3036)
                     /gene="gcvPA"
                     /locus_tag="VFO88_02830"
     CDS             complement(1708..3036)
                     /gene="gcvPA"
                     /locus_tag="VFO88_02830"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET9544488.1"
                     /translation="MSLPYLSLTERDRDEMLAAIGVSSVEELFRDIPEAVRFRGDLEL
                     EPPLSEQEITAHLAELASRNAHTGVELSFLGAGIYDHYVPAFVDAMLARGEFLTAYTP
                     YQPEMSQGVLQAIFEYQTAVCELTGMDVSNASGYDGPAVTADACYVAKHVTGRSRVVL
                     CEAVSPQVRRVVKTYAPGFGLEVVEVPHRDGVTDPEELRAAAADAACVVFQQPNFLGN
                     LEPAPELAAAANEQEALPVAYVDPMSLGLLEAPGRYGCAIAVGEGQPAGNYQSYGGPH
                     YGFLAAREDYVRRMPGRIVGETTDLDGRRAFVLTLQTREQHIRREKATHNITTNQTLL
                     ALAGLMYLSWLGPQGLRQVGETCLALAQYAKERLGFPLVFPEEATFKEFAVRVGRPAR
                     EAVAAARAEGVHPGYALGRDYEGLDDALLVCVTEKRTREDIDRLAEVLRT"
     gene            complement(3033..3428)
                     /gene="gcvH"
                     /locus_tag="VFO88_02835"
     CDS             complement(3033..3428)
                     /gene="gcvH"
                     /locus_tag="VFO88_02835"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9544489.1"
                     /translation="MAADESYPTELKYHAEHDWARIEGDTATLGITWYAQDALGEIVH
                     FEPPEEGGSVAAGESYGEVESVKAVSDLIAPLSGEILEVNPALADAPETINEDPYGEG
                     WLIRVRLTEPGEVDGLLDADAYRKLLEEQ"
     gene            complement(3428..>4519)
                     /gene="gcvT"
                     /locus_tag="VFO88_02840"
     CDS             complement(3428..>4519)
                     /gene="gcvT"
                     /locus_tag="VFO88_02840"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET9544490.1"
                     /translation="SEAARASKPPQARLEPLGSAPVQTLRRTPLHERHAALGARLVPF
                     AGWEMPVQYTSIAEEHAAVRSSAGVFDVSHMGELELAGDGAHAYLQGRLTNDLDRIEP
                     GRAQYTLLTNAEGGIVDDLIAYRRGEDDYLLVVNASNREPDHAALPETRDVSDGWALL
                     AVQGPEALDRLGIEIAPFTFRDDDVLGVRCLVAGTGYTGERGCELGCDPADAPRLWDA
                     ILERGIVPCGLAARDTLRLEACYPLHGNDIGPDRTPIEAGLGWACALDKEFPGVEVLR
                     RQKEEGPAQKLVAFRMEDKGIPRQGMAIAEGGEVTSGTLSPTLGEPIGLAYVDARLAE
                     AGTEITIDLRGRPRRARIARKPFYEREER"
BASE COUNT          596 a         1617 c         1640 g          666 t
ORIGIN      
        1 aggacgacgt agagctcttc cgtctccgcg tggtagtgcc gctcggtcgc ctgccccggc
       61 gcgagcgtcg cctcggcgag gctctggttc cgcgcgccgc ccgcctcggc gtcgacgagg
      121 acgcggatcg tcgatccgtc cgcggtcgtg aacggctcgg cgtcggcgcg cgagcgcacc
      181 tccacggtca gacgcccttg ggtgcctcga ccggctgcgc cggctcgcgc gccgctcccg
      241 cgtcggggtg ctcgtcgaac cggtaccgga cgatcgcctg cttcgccgcc ttcacctcgt
      301 cgagccgctt caccggccgc cggtgcggcg cgtccttcac gagcccgggc tcctcggccg
      361 cctcgcgcgc gatggcgagc atcgcgtcgg cgaacgcgtc gagccgctcc ttcggctccg
      421 tctcggtcgg ctcgatcatg agcgcctccg ggacgatgag cgggaagtag atggtcggcg
      481 ggtggaagcc atagtccatg agccgcttcg cgacgtcgag cgcggtcacc ccgtgctcgc
      541 gcttgagggt gcgcgcggag aggacgaact cgtgcatgca caggcggtcg tacggcaggt
      601 cgtaggcgtc acgcaggcgc gcgaggaggt agttcgcatt cagcaccgcg acctcggaca
      661 tctcgcgcag gcgcggcccg tacgaccgga tgaacgcgta cgaccggacg aacacgccga
      721 acggcccgta gtagccgcgc accttcccga tcgtcttcgg gcggtcctcg tcgaaccgga
      781 agcgctcgcc gtcgcggacg acgaccggga ccgggaggaa cggctcgagc tgcgcccgca
      841 ccgcgaccgg cccgccgccc ggcccgccgc cgccgtgcgg ctgcgagaac gtcttgtgga
      901 ggttgtagtg gacgatgtcg aagcccatgt cgcccggtcg cgagatcccg catacggcgt
      961 tcaggttcgc gccgtcgtag tagaggagcg cgccggcgcc gtggaagatg tccgccacct
     1021 cctcgatccg ctcgtcgaag agcccgagcg tcgaggggtt cgtgagcatg aggccggccg
     1081 tgcgctcgtc gacgtgctcg cggaggaagt cgacgtcgag gttcccgcgc tcgtccgtgc
     1141 cgaccttctg gagcttgtag ccggccatcg tgacgctcgc cgggttcgtc ccgtgggcgg
     1201 tgtcggcgac gaagacggtg tcccgcccct ccccctcgcc gcggtcctcg aagtacgcgc
     1261 gcatgagcat gagcccggtc agctcgccct gcgagcctgc cgcgggttgg agcgagaccg
     1321 cgtcgagccc gacgacctcg gcgaggatct cctggaggcg ccacatgagc tcgagcgcgc
     1381 cctgggctcc gtcctcctcc tggagcgggt ggaggtcgcg gaagccgggc agcgcgacga
     1441 cgcgctcgtt cacgcgcggg ttgtgcttca tcgtgcagga gccgagcggg tagaagccgg
     1501 tgtcgacgcc gaacgtccgg tctgcgagcg cggtgaaatg gcggacgagc tccggctcag
     1561 agagctcggg gagccgcggc gcctgctcgc gccggagggc ctcgggcagc tcgggaacgg
     1621 gcaggcccgg gtcgggcaac gtcgcggcgc gccgtccggg ctgcgacttc tcgtacacga
     1681 gctgcatggg cggacgcgga tcggcggtca cgtgcggagc acctcggcga gccggtcgat
     1741 gtcctcgcgc gtccgcttct ccgtcacgca gacgaggagc gcgtcgtcga ggccctcgta
     1801 gtcgcgcccg agcgcgtagc cgggatgaac gccctcggcc cgggcggcgg cgacggcctc
     1861 gcgggcggga cgtccgacgc gtacggcaaa ctccttgaac gttgcctcct cgggaaacac
     1921 gagtggaaag cccaggcgct ccttcgcgta ctgcgcgagc gcgaggcagg tctcccccac
     1981 ctgccgcaat ccctggggcc cgagccagga gaggtacatg agcccggcca gcgcgaggag
     2041 cgtctggttc gtggtgatgt tgtgcgtcgc cttctcgcgc cggatgtgct gctcacgcgt
     2101 ctggagcgtg aggacgaacg cgcgccggcc gtcgaggtcg gtcgtctcac cgacgatccg
     2161 gcccggcatc cggcggacgt agtcctcccg cgccgcgagg aacccgtagt gcgggccgcc
     2221 gtacgactgg tagttgccgg cgggctgtcc ctcgccgacg gcgatcgcgc agccgtaccg
     2281 cccgggcgcc tcgaggaggc cgagcgacat cgggtcgacg tacgcgacgg gcagcgcctc
     2341 ctgctcgttc gcggccgcgg ccagctccgg ggcgggctcc aggttcccga gaaaattggg
     2401 ctgctggaag acgacgcacg cggcatccgc cgccgcggcg cggagctcct ccggatcggt
     2461 cacaccgtca cggtgcggca cctcgacgac ctcgaggccg aaccccggcg cgtacgtctt
     2521 gacgacgcgg cgcacctgcg ggctcacggc ctcgcagagg acgaccctcg agcggccggt
     2581 gacgtgcttc gccacgtagc acgcgtccgc cgtcacggcc gggccgtcgt agccggacgc
     2641 gttcgagacg tccatgccgg tcagctcgca gaccgccgtc tggtactcga agatcgcctg
     2701 gaggacgccc tggctcatct ccggctggta cggggtgtac gcggtcagga actcaccgcg
     2761 cgcgagcatc gcgtcgacga acgccgggac gtagtggtcg tagatccccg cgccgaggaa
     2821 cgatagctcg acgccggtgt gcgcattgcg tgaggcgagc tccgccaggt gcgccgtgat
     2881 ctcctgctcg gagagcggcg gctcgagctc aaggtcgccg cggaagcgca ccgcctcggg
     2941 gatgtcccgg aagagctcct cgaccgacga gacgccgatc gcggcgagca tctcgtcgcg
     3001 gtcgcgctcc gtcagggaga ggtacgggag gctcactgct cctcgaggag cttgcggtag
     3061 gcgtcggcgt cgaggaggcc gtcgacctcg cccggttcgg tcaggcgcac acggatgagc
     3121 cagccctcgc cgtacgggtc ctcgttgatc gtctcgggcg cgtcggcgag cgccgggttg
     3181 acctcgagga tctcgccgga gagcggcgcg atcaggtcgg acacggcctt cacggactcg
     3241 acctcgccgt agctctcccc cgcggccacg ctgccgccct cctcgggagg ctcgaagtgg
     3301 acgatctcgc cgagtgcgtc ctgggcgtac cacgtaatcc cgagggtcgc cgtgtcgccc
     3361 tcgatccggg cccagtcgtg ctcggcgtgg tacttgagct cggtcggata gctctcgtcg
     3421 gctgccacta gcgctcctct cgctcgtaga acggcttcct ggcgattctc gcgcggcggg
     3481 gacggccacg caggtcgatc gtgatctccg tgccggcctc cgcgagccgg gcgtcgacgt
     3541 acgcgagccc gatcggctcg ccgagcgtcg gcgagagcgt cccggacgtg acctcgcccc
     3601 cctcggcgat cgccatgccc tgccgcggga tccccttatc ctccatccgg aacgcgacga
     3661 gcttctgcgc cgggccctcc tccttctggc gccggagcac ctcgacgccc gggaactcct
     3721 tgtcgagcgc gcacgcccag ccgaggcccg cctctatcgg cgtccggtcg gggccgatgt
     3781 cgttcccgtg cagcgggtag cacgcctcga gccggagcgt gtcgcgcgcg gccaggccgc
     3841 acgggacgat cccgcgctcg aggatcgcgt cccacaggcg cggcgcgtcg gccgggtcgc
     3901 agccgagctc gcacccccgt tcccccgtgt agccggtccc cgcgacgagg caccggacgc
     3961 cgagcacgtc gtcgtcgcgg aaggtgaacg gcgcgatctc gatcccgagc cggtcgagcg
     4021 cctccgggcc ctgaaccgcg aggagcgccc agccgtcgga gacgtcccgc gtctcgggca
     4081 gcgccgcatg gtcgggctcc cggttcgacg cgttcaccac gaggaggtag tcgtcctcgc
     4141 cgcgccggta ggcgatcagg tcgtcgacga tcccgccctc ggcgttcgtg agcagcgtgt
     4201 actgggcgcg cccgggctcg atccggtcga ggtcgttcgt cagccggccc tggaggtagg
     4261 catgcgcgcc gtcgccggcg agctcgagct cgcccatgtg cgagacgtcg aagacacccg
     4321 cgctcgagcg gacggcggcg tgctcctccg cgatggacgt gtactgcacc ggcatctccc
     4381 agcccgcgaa cgggacgagg cgggcgccga gcgcagcgtg gcgctcgtgg agcggcgtgc
     4441 ggcggagcgt ctgcaccgga gcggatccta gaggctcgag ccgcgcctgc ggcggcttcg
     4501 aagcgcgcgc agcctcgga
//
LOCUS       DASTFC010000098         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1122331,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000098 DASTFC010000000
VERSION     DASTFC010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122331"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..822
                     /locus_tag="VFO88_02845"
     CDS             49..822
                     /locus_tag="VFO88_02845"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9544491.1"
                     /translation="MGRLDGKVVVITGAAGGIGREAALLFSGEGARVCVADVDPDAGE
                     QTASECRDAFFHEVDVASEESVRALYETTAERYGGIDVLYNNAGIMPADDASVLDTGA
                     DAWERVLAVNAKGVFLCCKHGIPHLLERGGGSVINVASFVALMGAATSQIAYTASKGA
                     VLSLSRELAVEFARRGVRVNALCPGPVETPLLMRLFSDDPAAYERRRVHLPMGRLAQA
                     REVAQAALFLASDEASYVNGATFLVDGGLSAAYVTPLDE"
     gene            complement(803..1342)
                     /locus_tag="VFO88_02850"
     CDS             complement(803..1342)
                     /locus_tag="VFO88_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544492.1"
                     /translation="MDERERKRLLGAWGVRGENDRERREREVLERDLAGAPYHGQRVR
                     LRPNPFGPATESYVTSLGGPLPYMVRLREIEVETAAHERALAEAWQELAAECAGRPDT
                     FARRWRSLAARWNFVAVNELIERHNRWYPTEARLPMDLRTRDFALVNGEHYRREPLGP
                     DWVLRRFPAELELATRRAA"
     gene            complement(1365..>2949)
                     /locus_tag="VFO88_02855"
     CDS             complement(1365..>2949)
                     /locus_tag="VFO88_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544493.1"
                     /translation="DADYVELVARLERDFMTAVQKNVRARIFLNLWKNLLMAKPSYSR
                     SDRIGDLLDRLFYKPAWPEPRPKRRWGLLDTQIALTELRIQPAGDDRDFRPDEHNAGR
                     ANKVPMLMINATSLNTGHNWRFEAVRMGEPMPRDARTRRVAAEVDKNRRLDLGYYVPD
                     EGPNGHKAITSRQREFPLGLAVAASACVPTLFHPLAISGLYDGLRVELVDGGVHDNQG
                     VQALFDADCTHLVVSDASGQMADLDWPATRIPGVAGRSISIYGDRIRDEQLMHATNRA
                     QPIALVHLRKGLPAEAVAPLADDGSPVREIPEEERVGPVRSADFGVLEDTQRRLARVR
                     TDLDAFGDTESFSLMLDGYLMSDLELRTCTPLEPLLAPDALGADPGRWRFGAVAPHIG
                     WADPPAWYRRALAASDKRFFKPLTLVPVLRWTLLALVTAAVLLLALLVVGNAGGLEDW
                     LTGSWPRWLTFAILGGLAVFTFAYVREQTRWRLLRLASTLLISVLMPILAAPILWLSS
                     LVTVGLTPLFLRLGRVRDG"
BASE COUNT          413 a         1012 c         1138 g          386 t
ORIGIN      
        1 acgaccgggt agattccgcc gaccggcaag gcggaaagga ggcggctcgt gggcaggctg
       61 gacgggaagg tcgtggtgat cacgggggcg gccggaggga tcgggcgcga ggccgcgctc
      121 ctcttctcgg gcgaaggcgc ccgggtctgc gtcgccgacg tcgacccgga cgcgggggag
      181 cagacggcct ccgagtgccg ggacgcgttc ttccacgagg tcgacgtcgc gagcgaggag
      241 agcgtccggg cgctctacga gacgaccgcc gagcggtacg gcggcatcga cgtcctctac
      301 aacaacgcgg ggatcatgcc ggcggacgac gcgtccgtcc tcgacacggg cgccgacgcg
      361 tgggagcgcg tgctcgccgt gaacgcgaag ggcgtcttcc tgtgctgcaa gcacgggatc
      421 ccgcacctgc tggagcgcgg cggcggatcg gtgatcaacg tcgcgtcgtt cgtcgccctc
      481 atgggtgcgg ccacctcgca gatcgcgtac acggcgtcga agggagccgt cctctcgctc
      541 tcgcgggagc tcgcggtcga gttcgcgcgc cgcggcgtcc gcgtgaacgc cctctgcccc
      601 gggcccgtcg agacgccgct cctcatgcgg ctcttctccg acgacccggc ggcgtacgag
      661 cgccggcgcg tccacctccc gatggggcgg ctcgcgcagg cgagggaggt cgcgcaggcg
      721 gcgctcttcc tcgcaagcga cgaggcctcg tacgtgaacg gggcgacgtt cctcgtggac
      781 ggcggcctga gcgccgccta cgtcacgccg ctcgacgagt agccagctcg agctcggccg
      841 ggaagcggcg cagcacccag tccggcccga gcggctcccg ccgatagtgc tcgccgttca
      901 cgagggcgaa gtcgcgcgtg cgcaggtcca tcggaaggcg cgcctcggtc gggtaccagc
      961 ggttgtggcg ctcgatcagc tcgttcaccg cgacgaagtt ccagcgcgcg gcgagcgagc
     1021 gccagcggcg ggcgaacgtg tccggccggc cggcgcactc ggcggcgagc tcctgccacg
     1081 cctcggcgag cgcccgctcg tgcgccgccg tctcgacctc gatctcgcgc aggcgcacca
     1141 tgtacgggag cggcccgccg agcgaggtga cgtacgactc cgtcgccggg ccgaacgggt
     1201 tcggacgcag gcggacgcgc tgcccgtggt acggcgcgcc cgcgaggtcg cgctcgagca
     1261 cctcgcgctc ccgccgctcg cggtcgttct cgccgcgcac gccccaggcg ccgagcagcc
     1321 gcttccgctc gcgttcgtcc atgccctgat tgtgcccgcc cgggctaccc gtcgcggacg
     1381 cggccgagcc ggaggaagag cggggtcagc ccgaccgtga cgagcgacga gagccagagg
     1441 atcggcgccg cgaggatcgg catgaggacg gagatgagga gcgtgctggc gaggcggagc
     1501 aggcgccagc gcgtctgctc gcgcacgtac gcgaaggtga agaccgcgag tccgccgagg
     1561 atcgcgaacg tgagccagcg cggccaggag cccgtcagcc agtcctcgag cccgcccgcg
     1621 ttcccgacga ccagcagggc gaggagcagg acggcggccg tgacgagcgc gaggagcgtc
     1681 cagcgcagga cgggcacgag cgtgagcggc ttgaagaagc gcttgtccga ggcggcgagg
     1741 gcgcggcggt accaggcggg cgggtcggcc cagccgatgt ggggcgcgac cgcgccgaac
     1801 cgccagcggc cgggatccgc tcccagcgcg tccggcgcga ggagcggctc gagcggcgtg
     1861 caggtgcgaa gctcgaggtc gctcatgagg tagccgtcga gcatgagcga gaacgactcg
     1921 gtgtccccga aggcgtcgag atccgtgcgc acgcgggcga gccgccgctg cgtgtcctcg
     1981 agcaccccga agtcggccga gcgaacggga ccgacgcgct cctcctccgg gatctcccgc
     2041 acgggcgagc cgtcgtccgc gagcggcgcg accgcctcgg ccggaaggcc cttgcggaga
     2101 tgaacgagcg cgatcggctg cgcgcggttg gtcgcgtgca tgagctgctc gtcgcggatc
     2161 cggtcgccgt agatcgagat cgagcgcccg gccacgccgg ggatgcgggt ggccggccag
     2221 tcgagatcgg ccatctgccc cgacgcgtcg ctcacgacga ggtgcgtgca gtcggcgtcg
     2281 aagagcgcct gcacgccctg gttgtcgtgc acgccgccgt ccacgagctc gacgcgcagc
     2341 ccgtcgtaga gcccgctgat cgcgagcgga tggaagagcg tcgggacgca ggcggacgcc
     2401 gcgacggcga gcccgagcgg gaactcgcgc tggcgcgagg tgatcgcctt gtgcccgttc
     2461 gggccctcgt cggggacgta gtagccgaga tcgagccgcc ggttcttgtc cacctcggcg
     2521 gcgacccgcc gcgtgcgcgc gtcgcggggc atcggctcgc ccatgcgcac ggcctcgaag
     2581 cgccagttgt ggccggtgtt gagcgacgtc gcgttgatca tgagcatcgg aaccttgttc
     2641 gctcggccgg cgttgtgctc gtcggggcgg aagtcgcggt cgtcgcccgc cggctggatg
     2701 cgaagctcgg tcagcgcgat ctgcgtgtcg aggagccccc agcggcgctt cgggcgcggc
     2761 tccggccagg ccggcttgta gaagagccgg tcgagcaggt cgccgatccg gtcggagcgc
     2821 gagtacgagg gcttcgccat caggaggttc ttccagaggt tgaggaagat ccgggcgcgg
     2881 acgttcttct gcaccgcggt catgaagtcg cgctccaggc gggcgacgag ctcgacgtag
     2941 tcggcgtcg
//
LOCUS       DASTFC010000099         7112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_924599,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000099 DASTFC010000000
VERSION     DASTFC010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924599"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFO88_02860"
     CDS             complement(<1..433)
                     /locus_tag="VFO88_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET9544494.1"
                     /translation="MKIFVVGAGQVGTAVVEALYEEHDVVVLDVDGGRLQAVAHRFDV
                     VTVQGNGASRRALREAGVDKGDLLLACTSRDEVNIVASMFAKKLGPNIRTIVRTTNVE
                     YLEIWRERELEIDFMVCSELEAAHAIARLVGVPAARQTDVFA"
     gene            complement(744..989)
                     /locus_tag="VFO88_02865"
     CDS             complement(744..989)
                     /locus_tag="VFO88_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9544495.1"
                     /translation="MAYLRLIDVDEATGELRVEYDAAIGRAGKVFNIVKAMSLRPPVL
                     REAMGLYKAVMFGRCELSRAERELLAVVVSSANDCHY"
     gene            1032..1586
                     /locus_tag="VFO88_02870"
     CDS             1032..1586
                     /locus_tag="VFO88_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544496.1"
                     /translation="MRPRNVTVVGQGDEHEPLPDEGVVRVETPRSPYEDLGARVAGVL
                     AAAEQAAAQMKDEAETEAEQVRREADEYAKEVRAAVEAYAKEQRRRAEDEARAAVAAA
                     DAEARAMREAAQAMAARLEEEARRGTLKFREDTRALEERRQRVLDELREIAATLEDIA
                     QGGAAGRFGAPESIANARSRRRSR"
     gene            1583..2278
                     /locus_tag="VFO88_02875"
     CDS             1583..2278
                     /locus_tag="VFO88_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9544497.1"
                     /translation="MSRAGNTTGVGAVVFDLDGVIVDSEHVWNEARMRLAEERGGLWH
                     PGAQRAMMGMSSTEWSRYMHEVIELPDPPEEISAEVVRRLSAIYREELPLIPGAVEAV
                     RRLRARFRLGLASSSNRELIDLVLDLAGIAECFEATVSSEEVARGKPAPNVYLEVARL
                     LELPPEDCAAIEDSENGILSARRAGMRVVAVPNPRYPPGEAALAEADVVVRSPAELTL
                     SLVGSLPPRSWRG"
     gene            complement(2328..2684)
                     /locus_tag="VFO88_02880"
     CDS             complement(2328..2684)
                     /locus_tag="VFO88_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544498.1"
                     /translation="MRKVTVLATVVALGVAVLALAGGAQSRATSPPNTQIHGGPTGTV
                     TSRTATFHLYSTKPGRIRCKLNARPWRVCVNARQGYVTFRDLKRGKTHTLRAQAVDRQ
                     GRRDPTPAVRRWTVSL"
     gene            2828..3040
                     /locus_tag="VFO88_02885"
     CDS             2828..3040
                     /locus_tag="VFO88_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544499.1"
                     /translation="MLLRIDNPASADDLVRFFETHDYVVERRADTLVHVRPSSALGER
                     PDRERVVRDLEAWQAWNPGARAELVD"
     gene            complement(3108..4337)
                     /gene="fabF"
                     /locus_tag="VFO88_02890"
     CDS             complement(3108..4337)
                     /gene="fabF"
                     /locus_tag="VFO88_02890"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9544500.1"
                     /translation="MRRVAVTGMGAVTPVGNDVETTWNALVAGESGIDTITAFDPSEF
                     PVRIAGEVKGLEPTDVMSAKKARHVDRAVLFAVAAAREAMGDAGMNGFTPERVGVVVG
                     CCVGGINQSLQQWEVLKERGWDRMSPFFLANFLPDSPSGHLAIELGLKGPNCAVVSAC
                     ASGSHSIAEGAELIRTGQADAVLAGGTEGAISPLVLGGFCAMRGLATRNDEPARASRP
                     FDAGRDGFVMSEGAAVLMLEDYDTAEKRGATIYAEVLGAGSSNDAHNMIQPDPTGTGV
                     AAMMSTALETAGLEPEQVDYVNAHGTATPQGDIAETKAIKQVFGDHAYDLTISSTKSM
                     IGHLFGAAGAIEALVCARAIHEGVVPPTINLDDPDPDCDLDYVPNVARKEKVDVALSN
                     AMGLGGHNACVLLGRAS"
     gene            complement(4370..4639)
                     /locus_tag="VFO88_02895"
     CDS             complement(4370..4639)
                     /locus_tag="VFO88_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544501.1"
                     /translation="MWKVVRESGALPPFGVSKRIFGAAGWTLLAGAPAAHFKVRRPDS
                     DHAVLDYVGLPVRDELVARDSTTWDGRGVLLGREFCRFRLEREDV"
     gene            4675..5211
                     /locus_tag="VFO88_02900"
     CDS             4675..5211
                     /locus_tag="VFO88_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544502.1"
                     /translation="MDAYDVVNALLGVLYILLSLVLARYAVRLGRSFPWFALLVVLFF
                     ARGVDRVYAAFTDEQRLGILVDGILIAVLVALILGIDRTASALKTAHDDAAVREEEYR
                     RALTDYRRLARHRLANPLAAIRGSIVTLRDVPELDAEKRRQLLDALESEAIRLEKIAL
                     DPEVIGPEERGLDPRPNL"
     gene            5253..5642
                     /locus_tag="VFO88_02905"
     CDS             5253..5642
                     /locus_tag="VFO88_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544503.1"
                     /translation="MRLTAFVPVLWLAGVLGLLAAAGWERGRGDVAEAGSKPVAVAEG
                     RAAAPTANATKPTKLWRGKKGVRYERSYRICSVFTVKEVARELGVPARRKQAALAHAN
                     AWYDPPFRQAAYRGCLDAFRGRPPALG"
     gene            5639..6661
                     /locus_tag="VFO88_02910"
     CDS             5639..6661
                     /locus_tag="VFO88_02910"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9544504.1"
                     /translation="MSSDELPVPAGLPFPPMEAELVRELPEGDGWQYEPKWDGFRGVL
                     ENLDGELHLWSRNGRPLLRYFPELAGLGDRLPPRSALDGEVVVEREGALDFDSLQMRL
                     HPAESRVRKLSAEIPARYVCFDVLVWEGEDVWPLPLEERRARVEALPFDVSPATGELA
                     VARGWEESLEAAGFDGVIAKRLGSPYLPGSREAVVKVKRHLAADCVVIGVTWSEKGAG
                     FASLMLGLWDGDALRPVGTASAAGKRREEIEAAVLPLLGPSPERRPRGEPSRWRRDVT
                     LEWSPVEPKLVVEVRYDKWQKGRFRHGTRFLRFRPDKDPSACTVDQVRPKPGAGDVTV
                     PQLLGS"
     gene            complement(6676..>7112)
                     /locus_tag="VFO88_02915"
     CDS             complement(6676..>7112)
                     /locus_tag="VFO88_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544505.1"
                     /translation="ARSGAVCATPTRELERYFPSRAALQADDGRRVVVPARARGGAWW
                     ASFTASVRRPACARAPKRLTAVLDARSSAAGVRARLEARNPSTGRWVAVGSRRLGPGD
                     RLLAARVPASFARASAVVRLRVVATGPARFAAGLDLLRLDVR"
BASE COUNT          909 a         2578 c         2674 g          951 t
ORIGIN      
        1 ccgcgaagac gtccgtctgc cgcgccgcgg gcacgccgac gagccgggcg atcgcgtgcg
       61 ccgcctcgag ctccgagcag accatgaagt cgatctcgag ctcccgctcg cgccagatct
      121 cgaggtactc gacgttcgtc gtccggacga tcgtccggat gttcggcccg agcttcttcg
      181 cgaacatcga ggcgacgatg ttcacctcgt cgcgcgaggt gcacgcgagg agcaggtcgc
      241 ccttgtccac gcccgcctcg cggagcgcgc gccgcgacgc gccgttcccc tggacggtca
      301 cgacgtcgaa gcggtgcgcg accgcctgga gccgtccccc gtccacgtcg agcacgacca
      361 cgtcgtgctc ctcgtagagc gcctcgacga ccgccgtgcc gacctggcct gcgccgacga
      421 cgaagatctt cacggccggg agcctattcg tcccagtcgg gctcgggccc gatgccgacc
      481 gcctcggcga tccggttgat gtagttgaag aacgcgatca cctggatcgc gtcgtggatc
      541 gcctcgtcgg acaggccgtg gccgcggagc cggtcgatct ccgcctcgcc gaccgcggcc
      601 ggctcgagcg tcaggtcgac ggcgaagtcg cagagcgcgc ggaggcgcgc gtcgagcggc
      661 gcgcggcggt agtcgtggga gacgtgctcg gcaagcttgt cgtctgcgcc ctcggcacgg
      721 aggtcgtcgg catgcgcccg cgttcagtag tggcagtcgt tcgccgacga gacgacgacc
      781 gcgaggagct cgcgctcggc gcgcgagagc tcgcaccggc cgaacatgac cgccttgtag
      841 agccccatcg cctcgcgcag gacgggcggg cggaggctca tcgccttgac gatgttgaag
      901 accttgccgg cccgcccgat cgctgcgtcg tactcgacgc ggagctcgcc ggtcgcctcg
      961 tcgacgtcga tcagccggag gtaggccacg ggccccgatt ctgcccgacg acggtgtaga
     1021 ctcgcgctcc gatgcgccct cgcaacgtca cggtcgtcgg ccagggcgac gagcacgagc
     1081 cgcttcccga cgagggcgtc gtgcgggtcg agacgccccg ctccccgtac gaggacctcg
     1141 gcgcgcgcgt cgcgggcgtg ctcgcggcgg ccgagcaggc ggcggcccag atgaaggacg
     1201 aggcggagac cgaggcggag caggtgcgcc gcgaggccga cgagtacgcg aaggaggtcc
     1261 gcgccgccgt cgaggcgtac gcgaaggagc agcgccgccg cgcggaggac gaggcgcgcg
     1321 ccgcggtcgc ggccgccgac gccgaggcac gcgcgatgcg cgaggcggcc caggccatgg
     1381 cggcccgcct cgaggaggag gcacgccgcg gcacgctcaa gttccgcgag gacacgcggg
     1441 cgctcgagga gcgccgccag cgcgtgctcg acgagctccg cgagatcgcg gcgacgctcg
     1501 aggacatcgc gcagggcggc gcggccggcc ggttcggcgc gccggagtcg atcgcgaacg
     1561 cgcgctcccg gcgccgctcc cgctgagccg ggccgggaac accacggggg tcggcgcggt
     1621 cgtcttcgac ctcgacggcg tgatcgtcga ctcggagcac gtctggaacg aggcgcggat
     1681 gcggctcgcc gaggagcgcg gcggcctctg gcaccccggc gcccagcggg cgatgatggg
     1741 catgagctcc accgagtggt cgcgctacat gcacgaggtg atcgagctcc cggacccgcc
     1801 ggaggagatc tcggcggagg tcgtgcggcg gctgtcggcg atctaccgcg aggagcttcc
     1861 gctcatcccc ggggcggtcg aggcggtgcg gcgcctgcgg gcgcgcttcc ggctcgggct
     1921 cgcgtcgtcg tcgaaccgcg agctgatcga cctcgtgctc gacctggccg ggatcgccga
     1981 gtgcttcgag gcgaccgtct cctccgagga ggtggcgcgc gggaagccgg cgccgaacgt
     2041 ctacctcgag gtcgcccgtt tgctcgagct gccgcccgag gactgcgcgg cgatcgagga
     2101 ctccgagaac gggatcctga gcgcccgccg cgccgggatg cgcgttgtcg ccgtcccgaa
     2161 cccgcgctac ccgccgggcg aggcggcgct cgccgaggcg gacgtcgtcg tccggtcgcc
     2221 ggccgagctg acgctctcgc tcgtcggctc gctcccgccg cgctcgtggc gcggctgagc
     2281 ggctcccgca ggagggcttt gggattggat accgcgaagc gctcctacta gagcgagacc
     2341 gtccagcggc ggacggccgg cgtcgggtcg cggcggccct gccggtcgac ggcctgcgcg
     2401 cggagcgtgt gcgtcttccc gcgcttcagg tctcggaagg tgacgtagcc ctggcgcgcg
     2461 ttcacgcaga cgcgccacgg cctcgcgttc agcttgcagc ggatccggcc cggcttcgtg
     2521 gagtagaggt ggaaggtcgc cgtccgcgag gtcaccgtcc cggtgggccc gccgtggatc
     2581 tgcgtgttcg gcggcgaggt cgcccggctc tgcgccccgc cggcgagcgc gaggacggcg
     2641 acgccgagcg cgacgacggt ggcgagaacg gtgaccttcc gcatgggtgc tcctccccgg
     2701 ggttgtcgcg cacagaatcg cacgtccgca gcgcacggat cgcgcgagcg gtacgcacct
     2761 cgttccaggg atccggctcg cggcccggcg cccgcccgcc gacacacgga gtgggacttc
     2821 tgcggccatg cttcttcgca tcgacaaccc ggcctcggcc gacgacctcg tgcgcttctt
     2881 cgagacgcac gactacgtcg tggagcggcg cgccgacacg ctcgtccacg tccggccgtc
     2941 gagcgcgctc ggcgagcggc cggaccgcga gcgcgtcgtc cgcgacctcg aggcgtggca
     3001 ggcctggaac cccggcgcgc gcgccgagct cgtggactga gcggcgggcg ggagcgggtg
     3061 cccgcccgcc ggtcggtccg tgagttcggc cggcgccggc ttcgcgccta cgaggcgcgg
     3121 ccgaggagga cgcaggcgtt gtgcccgccg aggcccatcg cgttcgagag cgcgacgtcg
     3181 accttctcct tgcgggcgac gttggggacg tagtcgaggt cgcagtcggg atcggggtcg
     3241 tcgaggttga tcgtcggcgg gacgaccccc tcgtggatcg ccctcgcgca gacgagcgcc
     3301 tcgatcgccc cggccgcgcc gaagaggtgg ccgatcatcg acttcgtcga ggagatcgtc
     3361 aggtcgtacg cgtggtcgcc gaagacctgc ttgatcgcct tcgtctcggc gatgtcgccc
     3421 tgcggcgtcg cggtgccgtg cgcgttcacg tagtcgacct gctccggctc gagcccggcc
     3481 gtctcgagcg cggtgctcat catcgccgcg acgccggtgc ccgtcggatc gggctggatc
     3541 atgttgtgcg cgtcgttcga ggagcccgcg ccgagcacct ccgcgtagat cgtcgcgccg
     3601 cgcttctcgg ccgtgtcgta gtcctcgagc atgagcacgg ccgcgccctc ggacatgacg
     3661 aacccgtcgc ggccggcgtc gaagggtcgt gaggcgcggg ccggctcgtc gtttcgggtc
     3721 gcgagcccgc gcatggcgca gaagccgccc aggacgagcg ggctgatcgc gccctccgtc
     3781 ccgccggcga gcaccgcgtc ggcctggccc gtgcggatga gctcggcgcc ctcggcgatc
     3841 gagtgcgacc cggacgcgca ggcagagacg accgcgcagt tcgggccctt gagcccgagc
     3901 tcgatcgcga ggtggccgct cggcgagtcc ggcaggaagt tcgcgaggaa gaacgggctc
     3961 atccggtccc agccgcgctc cttcagcacc tcccactgct ggagcgactg gttgatcccg
     4021 ccgacgcagc agccgacgac gacgccgacc cgctccggcg tgaagccgtt catgccggcg
     4081 tcgcccatcg cctcgcgcgc ggccgcgacc gcgaagagca ccgcgcggtc cacgtgacgg
     4141 gccttcttcg ccgacatgac gtcggtcggc tcgaggccct tcacctcgcc ggcgatccgg
     4201 acggggaact cgctcgggtc gaaggcggtg atcgtgtcga tcccgctctc gccggcgacg
     4261 agcgcgttcc acgtcgtctc gacgtcgttt ccgacagggg tcaccgcccc catccccgtt
     4321 actgccacac gacgcatcta atggttggac gtgcccgggc cgcgccgagt tacacgtctt
     4381 cccgctcgag gcggaagcgg cagaactcgc gccccaggag cacgccgcgg ccgtcccagg
     4441 tcgtggagtc gcgcgcgacg agctcgtcgc ggacgggaag gccgacgtag tcgaggacgg
     4501 cgtggtccga gtcggggcgg cgcaccttga agtgcgcggc cggcgcgccg gcgaggaggg
     4561 tccagccggc cgcgccgaag atgcgcttcg agacgccgaa gggaggcagc gcgccgctct
     4621 cgcggacgac cttccacacg ccgtcgaggc gccccgtgcg gctaccttcg gtctgtggac
     4681 gcatacgacg tcgtcaatgc cctgctcggt gtcctgtaca tcctgctctc gctcgtcctc
     4741 gcgcgctacg ccgtgcgcct gggccggtcg ttcccctggt tcgcgctcct cgtcgtcctc
     4801 ttcttcgcgc gcggcgtgga tcgagtgtac gcggcgttca cggacgagca gcggctcggc
     4861 atcctcgtgg acgggattct gatcgcggtg ctcgtcgcgc tcatcctcgg gatcgatcgg
     4921 acggcgtccg cgctcaagac ggcgcacgac gacgcggccg tgcgggagga ggagtaccgg
     4981 cgcgcgctca cggactaccg gcggctcgcg cgccaccggc tcgcgaaccc gctcgccgcg
     5041 atccgcggga gcatcgtcac gcttcgggac gtcccggagc tcgacgccga gaagcgccgc
     5101 cagctcctcg acgcgctcga gtcggaggcg atccggctgg agaagatcgc cctcgacccg
     5161 gaggtcatcg ggcccgagga gcgcgggctc gacccgcggc cgaacctgta gcccgtttcg
     5221 ggcggcgccg cggcggaaat cccatcgcgg cagtgcggct cacggcattc gtccccgttc
     5281 tctggctcgc cggcgtcctc ggactgctcg ccgcggccgg gtgggagcgc gggaggggcg
     5341 acgtcgcgga ggcgggctcg aagcccgtcg ccgtggcgga gggccgcgcg gccgcaccga
     5401 ccgcgaacgc cacgaagccg acgaagctct ggcgcggcaa gaagggcgtc cgctacgagc
     5461 gctcgtaccg gatctgtagc gtcttcaccg tgaaggaggt ggcccgggag ctcggagtcc
     5521 ccgcgcggcg gaagcaggcg gcgctcgcgc acgcgaacgc gtggtacgac ccgcccttcc
     5581 ggcaggccgc ctaccgcggc tgcctggacg ccttccgggg tcgtccgccg gcgctcgggt
     5641 gagctcggac gagctcccgg tcccggccgg gctgccgttc ccgccgatgg aggcggagct
     5701 cgtgcgcgag ctgccggagg gcgacggctg gcagtacgag ccgaagtggg acggctttcg
     5761 cggcgtcctc gagaacctcg acggggagct gcacctctgg agccggaacg gacggcctct
     5821 cctgcgctac ttcccggagc tcgccgggct cggcgaccgg ctcccgccgc gctcggcgct
     5881 cgacggggag gtggtcgtcg agcgggaggg cgcgctcgac ttcgattcgc tccagatgcg
     5941 gctccatccg gcggagagcc gggtccggaa gctctcggcg gagatcccgg cgcgctacgt
     6001 ctgcttcgac gtgctcgtgt gggagggcga ggacgtctgg cccctgccgc tcgaggagcg
     6061 ccgcgcgcgc gtcgaggcgc tgccgttcga cgtctcgccc gcgacgggcg agctcgcggt
     6121 cgcgcgcggc tgggaggaga gcctcgaggc ggccggcttc gacggcgtga tcgcgaagcg
     6181 gctcgggtcg ccgtaccttc ccggctcgcg cgaggcggtc gtgaaggtga agcgccacct
     6241 ggccgcggac tgcgtcgtga tcggcgtgac gtggagcgag aagggcgcgg gcttcgcatc
     6301 gctcatgctc ggcctgtggg acggcgacgc gctccggccc gtcgggacgg cgtccgccgc
     6361 cgggaagcga cgggaggaga tcgaggcggc cgtgcttccg cttctcgggc cgtcgcccga
     6421 gcggcggccg cgcggcgagc cgagccgctg gcgccgcgac gtgacgctcg agtggtcgcc
     6481 ggtcgagccg aagctcgtcg tcgaggtgcg ctacgacaag tggcagaagg gccgcttccg
     6541 gcacgggacg aggtttctcc gcttccggcc ggacaaggac ccgtccgcgt gcacggtcga
     6601 ccaggtgcgt ccgaagcccg gcgcgggcga cgtgaccgtg ccgcagctgc tcggctccta
     6661 gccctggcgc gagcctcagc ggacgtcgag gcggaggagg tcgagccccg ccgcgaaccg
     6721 ggccgggccg gtcgccacga cgcgaagccg cacgaccgcg gacgcgcgcg cgaacgaggc
     6781 gggcacgcgg gccgcgagca gacggtcgcc gggcccgagc cgccgcgacc cgaccgccac
     6841 ccaccggccg gtggacgggt tccgcgcctc gagccgcgcc cgcaccccgg cggccgagga
     6901 acgcgcgtcg aggaccgccg tgagccgctt cggcgcccgc gcgcacgccg gccggcggac
     6961 cgacgccgtg aacgacgccc accacgcccc gccgcgcgcc cgcgccggca cgacgacccg
     7021 gcggccgtcg tccgcctgca gagccgcccg cgacgggaag taacgctcga gctcgcgcgt
     7081 gggcgtcgcg cagaccgccc ccgaccgcgc cc
//
LOCUS       DASTFC010000100         5891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1023372,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000100 DASTFC010000000
VERSION     DASTFC010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023372"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1183)
                     /gene="gmd"
                     /locus_tag="VFO88_02920"
     CDS             complement(146..1183)
                     /gene="gmd"
                     /locus_tag="VFO88_02920"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9544506.1"
                     /translation="MTTKRALITGITGQDGSYLAELLLAEGYEVAGMVRRASTESLER
                     IEHVLDRVALVQGDLLDQASLVSALGETEPHEVYNLAAQSFVPTSFRQPVLTAEFTAT
                     GVTRLLEAIRGVNPEIRFYQASSSEMFGRVREVPQNERTAFHPRSPYGVAKAYGHYIT
                     VNYRESYGLFACSGILFNHESPRRGLEFVTRKISDGVARLKLGLADELRLGNLDARRD
                     WGFAGDYVRAMWLMLQADEPDDFVVGTGAMHSVQDFVDAAFARAELDPEGLVAVDPAL
                     LRPAEVDQLVADPSHARDRLGWEPETSFEELVAMMVDADLERLSAGPRARAHGAADSL
                     GTAAFTDPERG"
     gene            complement(1180..2073)
                     /locus_tag="VFO88_02925"
     CDS             complement(1180..2073)
                     /locus_tag="VFO88_02925"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9544507.1"
                     /translation="MSGPVLVTGAGGFVGRHLLAELGGRAVAGRADVTDGDALAAELR
                     EVAPAAVCHLAAATSVAESWRRGAETWRVNVLGTVALLDAVRAEAPEARTLVVSTGEV
                     YGPTPDGPAGEDTPVAPLSPYAASKAAAELAATRAARAEKLDVVVARPFQHAGPGQDE
                     RFAIGSWTARIARLERAGGGDLEVGDLTVRRDLTDVRDVTRAYRLLLDPATPAGTYNV
                     CSGRTVELATVLDTLVRLAREPVEVRDNPALRRPVDVPVLAGDPARLEAATGWRAEIP
                     LEQTLADALDEARRRAAEEDA"
     gene            complement(2070..3197)
                     /locus_tag="VFO88_02930"
     CDS             complement(2070..3197)
                     /locus_tag="VFO88_02930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9544508.1"
                     /translation="MAQPLTERTAIVHDWFQGFHGAERVVEAMRAGLFADGNEPDVFT
                     FQAARELLPAELAARIRQESRLANLPGIRQRGHDPGRWRMLLPLMPGYFRRLDLAAYD
                     TVIASSHACAIHARPREGALHVCYCYTPIRYAWLPETEARRARGPAALGLRAARGWLR
                     RKDLEASRRPDGYIAISEAVRERIRTAYGRDAAVVHPPVDVGDFDPHAEKEPGRFLWA
                     HRLVAYKQPQVVAEAFRGLPHRLTMVGVGPLADELRRDLPPNVELRGWVSREELARLY
                     ERASGFIHVGEEDFGITMVEALAAGAPVVALARGGALDIVRPDEDGVLVERADPASVR
                     EAVERIAARAWDRDALAARAAEFSRERFLSRMLETIEGFRR"
     gene            complement(3290..4777)
                     /locus_tag="VFO88_02935"
     CDS             complement(3290..4777)
                     /locus_tag="VFO88_02935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9544509.1"
                     /translation="MSETSVVQRQHAGDPVLSAVPGVAAEASGIPAALTSAPAPSSLP
                     AAWRAARLGPWRDSLLRRLLALADLTTAALVALLFAAVPAGGPATALLAFAAAPAWVL
                     FAKLQGLYDGDERSLRHLTVDELPKLFVWALTCTVATLLVLWSAPVTPPSVGAGVAAF
                     GLAVVAATALRTTARFSWRRIVPREQAVIIGSGPLADEARRKLELFPDIHVDVVGQHD
                     ESVAGNGAVVGLEGADRVILASQSVDEALIAGLLRHCRAHHVKLSMIPPVRGMFGTAV
                     RLNHVADLPVVEYSTWGVSRSSLLLKRWIDVVLSTLALLVLAPLFLVVAAAVRLESRG
                     PAIFRQERAGQDGQPFRMLKFRTMVADAEARLPQLVSFESLRDPMFKLREDPRVTRVG
                     RILRRTSIDELPQLVNVLRGEMSLVGPRPEQVELVERYRPEHRFRLLVRPGITGPMQV
                     FGRGELTFEERLAVEREYIENLSLGRDVRILALTVSAVVHAKGAY"
     gene            complement(4845..>5891)
                     /locus_tag="VFO88_02940"
     CDS             complement(4845..>5891)
                     /locus_tag="VFO88_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9544510.1"
                     /translation="GSHLFAERRTPPLPGWAAAVAVLVVAVVAAVAVDAAERFGEFCD
                     PPPEFARGDFVQAHLVSGTGSGRCQFWGAALDQWREAPLAGQGAGSYEAWWSQNGTIP
                     YFIRDAHSLYLETLGELGVIGFLLLAGALATGLVAGARRLLAAADADEAVAIAAVLSG
                     FLAFLASAAIDWMWEETVVAVVGLLLLGLAVGPATSTAAPALAPAAESPRHSSLRRYG
                     AAIAVLVAGWALVCVQAVPLLTQYTIVASQEAVQDDDLPEALDQALAARQLSPWASSP
                     YLQLALVHEQGDDLPAARAAIGEAIERDPLNWRLWLIRARLETLDGEVAAARESLARA
                     VDLNPRSPLFANAG"
BASE COUNT          820 a         2101 c         2299 g          671 t
ORIGIN      
        1 ctgcgcgctc gccggcgatg ccgcgagggc gcccgcggcg agcaggcaga cgaaaaggac
       61 ggtgagccgc atctctctga gtgtatggac gaccccgcga cccgaaaccg gcggccgtca
      121 agcttcacaa agtgttgatg ccgcgctagc cgcgctccgg gtcggtgaac gccgccgtcc
      181 cgagcgagtc ggccgcgccg tgcgcgcggg cgcgggggcc cgcggagagg cgctcgagat
      241 cggcgtcgac catcatcgcg acgagctcct cgaaggaggt ctccggctcc cagccgagcc
      301 ggtcgcgcgc gtgcgacggg tcggcgacga gctggtcgac ctccgccggg cggagcaggg
      361 cggggtcgac ggccacgagc ccctcagggt cgagctcggc gcgcgcgaac gcggcgtcga
      421 cgaagtcctg gacggagtgc atggccccgg tgccgacgac gaagtcgtcc ggctcgtccg
      481 cctggagcat gagccacatc gcgcgcacgt agtcgcccgc gaagccccag tcccggcgcg
      541 cgtcgaggtt cccgagccgc agctcgtcgg cgagcccgag cttcaggcgc gcgaccccgt
      601 cggagatctt ccgcgtcacg aactcgagcc cgcgccgcgg cgactcgtgg ttgaagagga
      661 tcccggagca ggcgaagagc ccgtacgact cccggtagtt cacggtgatg tagtggccgt
      721 acgccttcgc gacgccgtac ggcgagcgcg ggtggaacgc cgtccgctcg ttctgcggca
      781 cctcgcgcac gcggccgaac atctcggagg aggacgcctg gtagaagcgg atctcgggat
      841 tcacgccgcg gatcgcctcg agcaggcgcg tcacgccggt cgccgtgaac tcggccgtca
      901 ggacgggctg gcggaagctc gtcgggacga agctctgcgc ggcgaggttg tagacctcgt
      961 gcggctccgt ctcgccgagg gccgagacga gcgaggcctg gtcgaggagg tcgccctgga
     1021 cgagcgcgac gcggtcgagg acgtgctcga tccgctccag gctctcggtc gacgcgcggc
     1081 gcaccatccc cgccacctcg tagccctccg cgaggaggag ctcggcgagg tacgagccgt
     1141 cctggcccgt gatccccgtg atgagcgcgc gcttcgtcgt catgcgtcct cctccgcggc
     1201 ccgccggcgc gcctcgtcga gcgcgtcggc gagggtctgc tcgagcggga tctccgcccg
     1261 ccagcccgtc gccgcctcga gccgcgccgg gtcgccggcg aggaccggga cgtccacggg
     1321 ccgcctcagt gcgggattgt cgcggacctc gaccggctcg cgcgcgaggc ggacgagcgt
     1381 gtcgagcacg gtcgcgagct cgaccgtgcg gccggagcag acgttgtacg tcccggcggg
     1441 cgtcgcgggg tcgaggagca ggcggtaggc gcgggtcacg tcgcgaacat cggtcaggtc
     1501 gcggcgaacc gtgaggtccc cgacctcgag gtcgccgccg cccgcgcgct cgagccgggc
     1561 gatccgggcc gtccacgagc cgatcgcgaa ccgctcgtcc tggcccgggc cggcgtgctg
     1621 gaacgggcgg gcgacgacga cgtcgagctt ctccgcgcgc gcggcccgcg tggcggcgag
     1681 ctcggcggcg gccttcgacg cggcgtacgg cgagagcgga gcgacgggcg tgtcctcacc
     1741 ggcggggccg tcgggcgtcg gcccgtatac ctcgccggtc gagactacga gcgtgcgggc
     1801 ctccggggcc tccgcgcgga cggcgtcgag gagcgcgacc gtgccgagga cgttgacgcg
     1861 ccacgtctcc gcgccgcggc gccacgactc ggcgaccgag gtcgcggcgg cgaggtggca
     1921 gacggccgcg ggagcgacct cgcggagttc ggcggcgagc gcgtcgccgt ccgtgacgtc
     1981 ggcccggccg gcgaccgcgc gcccgccgag ctccgcgagg aggtggcggc cgacgaaccc
     2041 gcccgcgccg gtgacgagca ccggcccgct caccgtcgga agccctcgat cgtctcgagc
     2101 atccgggaga ggaaccgctc ccgcgagaac tcggcagcgc gtgcggcgag cgcgtcgcgg
     2161 tcccaggcgc gcgcggcgat ccgctcgaca gcctcgcgga cggacgccgg gtcggcgcgc
     2221 tcgacgagca ccccgtcctc gtccggccgg acgatgtcga gcgccccgcc gcgcgcgagg
     2281 gcgacgacgg gcgcgccggc cgcgagcgcc tcgaccatcg tgatcccgaa gtcctcctcg
     2341 ccgacgtgga tgaagccgga cgcgcgctcg tacagccggg cgagctcctc gcgcgagacc
     2401 cagccgcgca gctcgacgtt cggcgggagg tcgcgccgga gctcgtccgc gagcgggccg
     2461 acgccgacca tcgtgagccg gtgcggaagc ccacggaacg cctcggcgac cacctgcggc
     2521 tgcttgtacg cgacgagccg gtgcgcccag aggaaacggc ccggctcctt ctccgcgtgc
     2581 gggtcgaagt cgccgacgtc gaccggcggg tggacgaccg cggcgtcgcg cccgtacgcg
     2641 gtgcggatcc gctcgcggac cgcctccgag atcgcgatgt agccgtccgg tcggcgcgag
     2701 gcctcgaggt ccttccggcg gagccagccg cgggcggcgc gcaggccgag cgccgccggg
     2761 ccgcgcgccc gccgcgcctc cgtctcgggg agccaggcgt aacggatcgg cgtgtagcag
     2821 tagcagacgt gcagcgcgcc ctcccgcggc cgggcgtgga tcgcgcacgc gtggctcgac
     2881 gcgatcacgg tgtcgtaggc ggcgaggtcg agccggcgga agtagccggg catgagcggg
     2941 aggagcatcc gccagcggcc ggggtcgtgc ccgcgctggc ggatccccgg aaggttcgcg
     3001 agccgcgact cctgccggat gcgggcggcg agctcggccg ggagcagctc gcgcgccgcc
     3061 tggaacgtga agacgtccgg ctcgttcccg tccgcgaaga gcccggcgcg catcgcctcg
     3121 acgacgcgct ccgcgccgtg gaagccctgg aaccagtcgt ggacgatcgc cgtccgctcc
     3181 gtcagaggct gcgccatttc gcggtcagct tttcacggag ggctcccggc ggcgcggagg
     3241 ggccgctagt aacactgtgt tactagcgag ccgccgttcg cggtcgccgc tagtaggcgc
     3301 ccttggcgtg gacgacggcc gagaccgtca gcgccaggat ccggacgtcg cggccgaggg
     3361 agaggttctc gatgtactcg cgctcgacgg cgaggcgctc ctcgaacgtg agctcgccgc
     3421 ggccgaagac ctgcatcggg ccggtgatcc ccggacggac gaggaggcgg aagcggtgct
     3481 ccggccggta gcgctcgacg agctcgacct gctcgggccg cgggccgacg agactcatct
     3541 cgccgcgcag cacgttcacg agctgcggga gctcgtcgat gctcgtccgg cgcaggatcc
     3601 ggccgacccg ggtcacgcgc gggtcttcgc ggagcttgaa catcgggtcg cggaggctct
     3661 cgaaggagac gagctgcggg agccgcgcct ccgcgtcggc gaccatcgtg cggaacttca
     3721 gcatccggaa cggttgcccg tcctgcccgg cgcgctcctg gcggaagatc gcgggcccgc
     3781 gcgactcgag ccggaccgcg gccgcgacga cgaggaagag cggcgcgagc acgaggagcg
     3841 cgagcgtgga gaggacgacg tcgatccagc gcttgaggag aagcgaggag cgcgagacgc
     3901 cccaggtcga gtactcgacc acggggaggt cggcgacgtg gttcagccgg accgccgtgc
     3961 cgaacatccc gcggacgggc gggatcatgc tcagcttcac gtggtgcgcc cggcagtgcc
     4021 gcaggaggcc ggcgatgagc gcctcgtcca ccgactgcga cgcgaggatc acgcggtcgg
     4081 cgccctcgag gccgacgacg gctccgttcc cggcgacgct ctcgtcgtgc tggccgacga
     4141 cgtcgacgtg gatgtccggg aagagctcga gcttgcgccg cgcctcgtcg gcgagcgggc
     4201 cgctgccgat gatgacggcc tgctcccgcg ggacgatcct gcgccaggag aagcgggcgg
     4261 tcgtgcggag cgcggtcgcc gcgacgacgg cgagcccgaa cgcggcgacg ccggcgccga
     4321 ccgagggcgg cgtgacgggc gcggaccaga gcacgagcag cgtcgcgacg gtgcacgtga
     4381 gagcccagac gaagagcttc ggcagctcgt cgaccgtcag gtggcgcagg ctgcgctcgt
     4441 ccccgtcgta gagcccctgg agcttcgcga agagcaccca cgcgggtgcc gccgcgaagg
     4501 cgagaagcgc cgtggcgggc ccgccggccg ggacggcggc gaagaggagc gcgacgagcg
     4561 cggccgtcgt gaggtcggcg agggcgagca ggcggcggag aagcgagtcg cgccacggcc
     4621 ccagccgcgc cgcccgccag gcggcgggaa ggcttgacgg cgcaggggcg ctcgtcagcg
     4681 ccgcagggat tccggaggcc tccgccgcca caccggggac ggcggagagc acggggtccc
     4741 cggcgtgctg ccgctgcaca accgaggtct cgctcattcg tcaccgagca tagtggacgc
     4801 gccccttccc ggacagaccg gatcgtgaac atctcgagaa gcgttcagcc ggcgtttgcg
     4861 aagagcggcg accgggggtt gaggtcgacc gcgcgcgcga ggctctcgcg ggccgcggca
     4921 acctcgccgt cgagcgtctc gagccgcgcc ctgatgagcc agaggcgcca gttcagcggg
     4981 tcgcgctcga tcgcctcgcc gatcgccgcg cgcgcggccg ggaggtcgtc gccctgctcg
     5041 tgcacgagcg cgagctggag gtacggcgag gacgcccagg gcgagagctg ccgcgcggcg
     5101 agcgcctggt cgagcgcctc gggaagatcg tcgtcctgca ccgcctcctg gctcgcgacg
     5161 atcgtgtact gcgtgaggag cggaaccgcc tggacgcaga cgagcgccca gccggcgacg
     5221 aggacggcga tcgccgcccc gtagcggcgg agggaggagt gccgcggcga ctcggcggcg
     5281 ggggcgagcg ccggcgcggc ggtgctcgtg gccgggccga cggcgaggcc gagcaggagc
     5341 aggccgacga cggcgacgac cgtctcctcc cacatccagt cgatcgcggc ggaggccagg
     5401 aaggcgagga agcccgagag cacggcggcg atcgcgacgg cctcgtccgc gtcggccgcg
     5461 gcgagcagcc gccgcgcgcc ggcgacgagg ccggtggcga gcgcgcccgc gaggagcagg
     5521 aacccgatca cgccgagctc gccgagcgtc tcgaggtaga gcgagtgcgc gtcgcggatg
     5581 aagtacggga tcgtcccgtt ctgcgaccac catgcctcgt acgacccggc gccctggccc
     5641 gcgagcggcg cctcgcgcca ctggtcgagc gcggcgcccc agaactggca gcggccgctt
     5701 cccgtcccgc tcacgagatg ggcctggacg aagtcgccgc gcgcgaactc cggcggcggg
     5761 tcgcagaact cgccgaaccg ctccgccgcg tcgaccgcga cggcggcgac gacggccacg
     5821 acgagcacgg cgaccgcggc cgcccagccg gggagcggcg gcgtgcggcg ttccgcgaag
     5881 aggtgcgagc c
//
LOCUS       DASTFC010000101         7421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1155284,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000101 DASTFC010000000
VERSION     DASTFC010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155284"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..913
                     /locus_tag="VFO88_02945"
     CDS             398..913
                     /locus_tag="VFO88_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9544511.1"
                     /translation="MLAGDLIELRLVREDDLGALYELMTDLAARGSYFPLGVLSEPAL
                     RAAYAKNGFWDKDEGMLLITAGEEIVGEIEFFPISHYMQGYELSYQLFGDRHAGKGYT
                     SEAVELLTRYLLAGKRVDRIQLVIHPDNAASKRVAAKCGFTFEGVMRRAWFHAGDFQD
                     VELWSRLRGEA"
     gene            927..1181
                     /locus_tag="VFO88_02950"
     CDS             927..1181
                     /locus_tag="VFO88_02950"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HET9544512.1"
                     /translation="MVVERFTQGARPVYERAAERGRMLPPGLVYVDSWVDESLSRCFQ
                     LMETDDPALLDAWIARWSDLAEFEVVPVLGSAEAAERALG"
     gene            complement(1193..2131)
                     /locus_tag="VFO88_02955"
     CDS             complement(1193..2131)
                     /locus_tag="VFO88_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9544513.1"
                     /translation="MRAALIREVGGVPEPAEVAAPAGETIEVLAAPINPIDLSVSRGI
                     LATGHPDLPYVPGCEAVGRTADGRTVWLFGGSLGRTVDGAIAERAAVGDAKAIEVPEG
                     VDPALASALGIAGLAGWLPLAWRAPLAGGETVLVLGATGSVGLVAVQAARLLGAARVV
                     AAGRSAAGLERAAECGADATIRLDEAGDLAAAFREAFDGAGPSYVFDPLWGEPAAAAV
                     RAAAPRATIVNLGQSAGATSELASADVRFKNLSLLGHTNFAVPEDELAAQYRRLVDHA
                     VAGEIRLDVERVPLADVADAWRRQSEGAGTKLVVVP"
     gene            complement(2131..3300)
                     /locus_tag="VFO88_02960"
     CDS             complement(2131..3300)
                     /locus_tag="VFO88_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544514.1"
                     /translation="MSGRLLVGLVLALAAVSVLVGACGGDSEPAPTTAAGIERCAELE
                     EDAEAARACYREELAALVSAAETPQDGAARADALAREQGGFLLANCHGIMHTVGREYA
                     RAEGVTLASLMDALPQSNDPGCSAGFAHGLVTGVAPEIDASSPETALEVCEDTETRYQ
                     RYSCVHGFGHAFMRISGEELDPALGLCSALGAEAPDCAQGAFHDYWFAVAGLDETEQP
                     AEVVDDPRRLCADQPERFVLPCWYRAYVDSRPPGEITVTADLQRLCAELEGIQREGCI
                     TAGSVIGPADPRSQLAMCAGLRGADAQACIHGVKAQNFLGQSIEADVALVERCELFAE
                     PAGRQECFRWLGKTLSVVTDGEFGEAGCPALTQADARRLCEQGAAAMDEPLVTFS"
     gene            complement(3312..4334)
                     /locus_tag="VFO88_02965"
     CDS             complement(3312..4334)
                     /locus_tag="VFO88_02965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9544515.1"
                     /translation="MELGLFSFVERTPGSGLTAEERLRNLLEEIELADQVGLDVFGVG
                     EHHRPDYVVSAPAVVLAAAAARTKRIRLTSAVNVLSSDDPIRVFQQFATVDLLSGGRA
                     EIMAGRGSFIESFPLFGYDLRDYDELFAEKLDLLLRLREGERVTWSGRHRPPLRDQPV
                     YPRPVQEPLPVWIAVGGTPESAVRAGTLGLPMALAIIGGLPERFVPFAELHRRAAEEA
                     GHGRLPLGINSHGFLADSADEAADLAFGPFSETMNRIGRERGWPPTTRVDFDAQRTLR
                     GALFVGSPAEVAEKILFQHSLFGHERFLLQLSVGSMPHDRMLRAIELFGTEVAPVVRA
                     ELGSPA"
     gene            complement(4404..5771)
                     /locus_tag="VFO88_02970"
     CDS             complement(4404..5771)
                     /locus_tag="VFO88_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HET9544516.1"
                     /translation="MRKRAPLVVLLGIATALVLMAPGYASKQADKRSPAVVQQAGMMK
                     AAAKGGKGKSKKPQTMQRCVRGTAGPYACKNIDMLSQVSMAELGLSFVNDTWGWIDPQ
                     TKREYALVGGIEGTVFVDITDKKRPKVLGILPAHTLDPQFPWWRDIKVYANHAYVVSE
                     QDGHGMQVFDLTQLRNDPAGYTIYSESAHYDEISWSHNLAINEDTGFAYPIGSGTCAG
                     GLHMVDITTPDNPTFAGCFDEHGYIHDTQCVVYNGPDSDWTGREICFNSNAEVHHNPP
                     PGSDPITNALSIVDVTDKANPVVISRTEYDGDGYSHQGWLTPNQAYFLHGDELDELEH
                     GNNTITRVWDVRDLDAPVMTLAWENPKTAAIDHNMYTKGKYVYQSNYSAGLRVLDISR
                     VSAPALREVAFFDVSPEDDLPHFDRGTWSNYPYWKNKHIVTVSSMDRGLFVLKTRALG
                     SKGKK"
     gene            complement(5875..7329)
                     /locus_tag="VFO88_02975"
     CDS             complement(5875..7329)
                     /locus_tag="VFO88_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET9544517.1"
                     /translation="MTAAENRTRWLALLVLCLGDVMIVLDGTIVNVALPSIRDDLGFT
                     QASLAWVVNAYMLTFGGFLLLGGRLGDLYGNRRLFLGGIVLFTLASLACGLATTQGML
                     VGARAVQGVGAAVVSAVALSLVMSLFTEPADRAKAMGVFGFVLSGGGTIGVLLGGVLT
                     DVLNWHWIFLVNLPVGIAVFLLGLRLLPGARAASASRRLDVPGAVTATAALMLAVYAI
                     VQGNEVGWTSGRTLGLLAAAVALLAVFFVIEARTPAPLVPLRIFRIRNVSTANVVGVL
                     MAGGMFAQFFFSALYLQQILGYSPLEVGIAFVPSTVLWGASSLFLSDRLVMRFGIKPP
                     LVAGLGLMAIALALFGRMPLDGNYLVDVLPATLLLGLGAGIAFNPLLLAAMSGVEPSE
                     SGLASGVVNTAFMMGGALGLAVLAASAAARTDSLLAAGRSELAALNGGFHVAFLLGAL
                     LVAGAAVLGGALLRGLAEEPPGHASPAEPALAAD"
BASE COUNT         1069 a         2660 c         2688 g         1004 t
ORIGIN      
        1 cgtccgcatg gacgagcacg gagccgtccg ccttcagcat gaggagccgc gtcgagggcg
       61 gcaggtacgc gttcagccgt cccgagtaga cgacctcgca gcgggcgacg acgagacgca
      121 cggggcgagg accgtaccgg ctctttcgga cgtgtccaaa gcgggcgacg gacggtgacc
      181 ttgtaacaca gtgttaccag ctggggctcc ttcccggcgt gccgccgggc cccgcgctcg
      241 cgcctacgcg atgcggggtg tggggacggc gaaattcatg tgacaggttc ttgcaggcgg
      301 ggtcaggtct tgcatcgtgc gccggccctc cgcggaatgt gatgtcgcaa ggcctgaccc
      361 ccggggttcg tcccgcgccg gaatagactc acgcgcggtg ctggccggtg acctcatcga
      421 gctcaggctc gtccgcgagg acgacctcgg cgcgctctac gagctcatga ccgacctcgc
      481 ggcgcggggc tcctactttc cgctcggcgt cctgagcgag cctgccctcc gcgcggccta
      541 cgcgaagaac ggcttctggg acaaggacga agggatgctg ctgatcaccg ccggcgagga
      601 gatcgtcggc gagatcgagt tcttcccgat cagccactac atgcagggct acgagctctc
      661 gtaccagctc ttcggcgacc ggcacgcggg caagggctac acgtccgagg ccgtcgagct
      721 gctcacccgc tacctcctcg cgggcaagcg cgtcgaccgg atccagctcg tgatccaccc
      781 cgacaacgcg gcctcgaagc gggtcgcggc gaaatgcggc ttcacgttcg agggcgtgat
      841 gcgccgcgcg tggttccacg ccggcgactt ccaggacgtc gagctctggt cgcggctgcg
      901 cggcgaggcc tgagtccgtg cgctacatgg tcgtcgagcg gttcacgcag ggcgcgcggc
      961 ccgtctacga gcgcgcggcc gagcgcgggc ggatgctccc gcccgggctc gtgtacgtcg
     1021 acagctgggt ggacgagagc ctgagccgct gcttccagct catggagacg gacgacccgg
     1081 cgctcctcga cgcctggatc gcccggtgga gcgacctcgc cgagttcgag gtcgtgcccg
     1141 tgctcggctc ggcggaggcg gccgagcggg cactcggctg acgccgcccg cgtcagggga
     1201 cgacgacgag cttcgtcccg gcgccttcgg actggcggcg ccaggcgtcc gcgacgtcgg
     1261 cgagggggac gcgctccacg tcgagccgga tctcgccggc gaccgcgtgg tccacgagcc
     1321 ggcggtactg cgccgctagc tcgtcctcgg ggacggcgaa gttcgtgtgg ccgaggagcg
     1381 acaggttctt gaagcggacg tccgcggacg cgagctcgct cgtcgcgccg gccgactggc
     1441 cgaggttcac gatcgtcgcg cgcggcgcgg ccgcccggac cgccgccgcg gccggctcgc
     1501 cccagagggg atcgaagacg tagctcggcc ctgccccgtc gaacgcctcc cggaaggcgg
     1561 ctgcgaggtc gccggcctcg tcgaggcgga tggtcgcgtc cgcgccgcac tccgcggcgc
     1621 gctcgagccc ggccgcgctg cgaccggccg cgacgacccg cgccgcgccg agcagccgcg
     1681 cggcctgcac ggcgacgagg ccgacggagc ccgtcgcgcc gaggacgagc acggtctcgc
     1741 cgcctgcaag cggcgcccgc caggccagcg gcagccagcc ggcgagcccc gcgatcccga
     1801 gcgcggacgc gagcgccgga tccacgccct cgggcacctc gatcgccttc gcgtcgccga
     1861 cggctgcgcg ctcggcgatc gcgccgtcga ccgtgcgccc gagcgagccg ccgaacagcc
     1921 agaccgtcct tccgtccgcc gtgcggccga cggcctcgca gcccggcacg tacggcaggt
     1981 cgggatgccc cgtcgcgagg atgccgcgcg agacggagag gtcgatcggg ttgatcggcg
     2041 cggcgagcac ctcgatcgtc tcgccggccg gcgcggccac ctcggccggc tcgggcacgc
     2101 cgccgacctc ccggatgagc gcggcccgca tcagctgaag gttacgagcg gctcgtccat
     2161 cgcggcggcg ccctgctcgc acaggcgccg cgcgtccgcc tgcgtgagcg cggggcagcc
     2221 ggcctcgccg aactcgccgt cggtcacgac gctcagcgtc ttcccgagcc agcggaagca
     2281 ctcctgccgc cccgccggct ctgcgaagag ctcgcagcgc tcgacgagcg cgacgtccgc
     2341 ctcgatcgac tgcccgagga agttctgcgc cttcacgccg tggatgcagg cctgcgcgtc
     2401 cgccccacgc agccctgcgc acatcgcgag ctgggagcgc gggtcggcgg ggccgatgac
     2461 cgagccggcc gtgatgcagc cctcccgctg gatcccctcg agctccgcgc agaggcgctg
     2521 gagatcggcg gtcaccgtga tctcgcccgg cgggcgcgag tcgacgtagg cgcggtacca
     2581 gcacgggagc acgaaccgct ccggctggtc ggcgcagagc cgccgcggat cgtcgacgac
     2641 ctcggccggc tgctccgtct cgtcgagccc cgccaccgcg aaccagtagt cgtggaacgc
     2701 gccctgcgcg caatccggcg cctcggcgcc gagcgccgag cagaggccga gcgccgggtc
     2761 gagctcctcg cccgagatcc gcatgaacgc gtgcccgaac ccgtggacgc agctgtagcg
     2821 ctggtagcgc gtctccgtgt cctcgcagac ctcgagcgcg gtctccgggg aggacgcgtc
     2881 gatctcgggc gcgaccccgg tcacgaggcc gtgcgcgaag ccggccgagc agcccgggtc
     2941 gttcgactgc gggagcgcgt ccatgagcga cgcgagcgtc acgccctccg cgcgggcgta
     3001 ctcgcgcccg accgtgtgca tgatcccgtg gcagttcgcg agcaggaagc cgccctgctc
     3061 gcgcgcgagc gcgtcggcgc gggcggcgcc gtcctgcggg gtctcggccg ccgacacgag
     3121 cgcggcgagc tcctcccggt agcacgcgcg ggcggcctcg gcgtcctcct cgagctcggc
     3181 gcagcgctcg atccctgcgg ccgtggtcgg cgcaggctcc gagtcgccgc cgcacgcgcc
     3241 gaccagcacg gacacggccg cgagggccag gacgaggccg accaggagtc tcccgctcac
     3301 ggccgcgaag gctatgcggg tgagccgagc tcggcccgca cgaccggagc gacctccgtg
     3361 ccgaagagct cgatcgcgcg cagcatccgg tcgtgcggca tcgagccgac gctgagctgg
     3421 agcaggaacc gctcgtgccc gaagagcgag tgctggaaga ggatcttctc cgccacctcg
     3481 gccgggctgc cgacgaagag cgcgccgcgg agcgtccgct gggcgtcgaa gtcgacccgc
     3541 gtcgtcggcg gccagccgcg ctcgcggccg atccggttca tcgtctcgga gaacggcccg
     3601 aaggcgaggt cggccgcctc gtccgccgag tccgcgagga agccgtgcga gttgatcccg
     3661 agcggcaggc gcccgtggcc cgcctcctcc gccgcgcgcc ggtggagctc ggcgaacggc
     3721 acgaagcgct cgggcagccc gccgatgatc gcgagcgcca tcggcagccc gagcgtcccg
     3781 gcacgcacgg cggactccgg cgtgccgccg accgcgatcc agacgggcag cggctcctgc
     3841 accggccgag ggtagacggg ctggtcgcga agcggcggcc ggtggcggcc cgaccacgtg
     3901 acccgctcgc cctcgcgcag ccgcaggagc aggtcgagct tctcggcgaa gagctcgtcg
     3961 tagtcgcgca ggtcgtagcc gaagagcggg aacgactcga tgaacgaccc gcgcccggcc
     4021 atgatctccg cgcgcccgcc cgagaggaga tccacggtcg cgaactgctg gaagacgcgg
     4081 atcgggtcgt ccgagctcag cacgttgacc gcgctcgtca ggcggatccg cttcgtccgc
     4141 gccgccgcgg cggcgagcac gacggcgggc gcggagacga cgtagtccgg ccggtggtgc
     4201 tcccccacgc cgaagacgtc gagcccgacc tggtcggcga gctcgatctc ctcgaggagg
     4261 ttcctcagtc gctcctcggc cgtgagcccg cttccgggcg tccgctcgac gaaggagaag
     4321 agcccgagct ccaccggcgg agttgtaccg gcccggaggg gcgccgcgcg cggcggcgcc
     4381 cctccgtccg gctcaggcct cggctacttc ttgcccttgc tgccgagcgc ccgcgtcttc
     4441 agcacgaaca ggccgcggtc catcgagctg accgtgacga tgtgcttgtt cttccagtac
     4501 ggatagttgc tccacgtccc gcggtcgaag tgcgggagat cgtcctccgg cgagacgtcg
     4561 aagaacgcca cctcgcggag cgcgggcgcg gagacccggc tgatgtcgag gacccgcagg
     4621 cccgcggagt agttcgactg gtacacgtac ttgcccttcg tgtacatgtt gtggtcgatc
     4681 gccgcggtct tggggttctc ccacgcgagc gtcatcaccg gggcgtcgag atcgcggacg
     4741 tcccagacgc gggtgatcgt gttgttgccg tgctcgagct cgtcgagctc gtcaccgtgc
     4801 aggaagtacg cctggttcgg cgtgagccag ccctggtggc tgtagccgtc cccgtcgtac
     4861 tccgtgcgcg agatcacgac cgggttcgcc ttgtcggtca cgtccacgat ggagagcgcg
     4921 ttggtgatcg gatcgcttcc gggcggcggg ttgtggtgca cctccgcgtt cgagttgaag
     4981 cagatctcac ggcccgtcca gtcggagtcg gggccgttgt agaccacgca ctgcgtgtcg
     5041 tggatgtacc cgtgctcgtc gaagcagccg gcgaacgtcg ggttgtcggg cgtcgtgatg
     5101 tcgaccatgt ggaggccgcc ggcacacgtg cccgagccga tcgggtacgc gaagccggtg
     5161 tcctcgttga tcgcgaggtt gtgcgaccag ctgatctcgt cgtaatgcgc ggactccgag
     5221 tagatcgtgt accccgcggg gtcgttccgg agctgcgtca ggtcgaagac ctgcatcccg
     5281 tgaccgtcct gctccgagac gacgtaggcg tggttcgcgt agaccttgat gtcacgccac
     5341 cacgggaact gcgggtcgag cgtatgcgcg ggcaggatcc cgagcacctt cgggcgcttc
     5401 ttgtccgtga tgtccacgaa caccgtgccc tcgatgccgc ccacgagcgc gtactcgcgc
     5461 ttcgtctgcg gatcgatcca gccccaggtg tcgttcacga agctgagccc gagctcggcc
     5521 atgctcacct ggctgagcat gtcgatgttc ttgcaggcgt acgggccggc cgtcccgcgg
     5581 acgcagcgct gcatggtctg gggcttcttg ctcttgccct ttccgccctt ggccgcggcc
     5641 ttcatcatcc cggcctgctg cacgaccgcc ggcgagcgct tgtccgcctg cttcgaggcg
     5701 tagcccggcg ccatcagcac gagcgctgtg gcgataccga ggagcacgac gagcggtgct
     5761 cgtttccgca tgtatctccc cctccctcct ggtgtgacgc cgccggctcc cgccggcggc
     5821 cctaacaggc cgggaagtat gccgaactag tgcgattttg gtcgagccaa gacgtcagtc
     5881 ggcggcgagc gcgggctcgg cgggcgaggc gtggccgggc ggctcctccg cgagcccgcg
     5941 gaggagcgcc cctcccagca cggccgcgcc cgccacgagc agcgcgccga gcaggaacgc
     6001 gacgtggaag ccgccgttca gcgccgcgag ctcgctccgg cccgcggcga ggagcgagtc
     6061 ggtgcgcgcg gcggcgctcg ccgcgagcac ggcgagcccg agcgcgccgc ccatcatgaa
     6121 cgcggtgttc acgacgccgg acgcgaggcc cgactcgctc ggctcgaccc cgctcatcgc
     6181 ggcgaggagc agcgggttga acgcgatccc ggcgccgagg ccgaggagga gcgtcgcggg
     6241 caggacgtcc acgaggtagt tcccgtcgag cggcatccgg ccgaagagcg cgagcgcgat
     6301 cgccatgagc ccgagcccgg cgacgagcgg cggcttgatc ccgaaccgca tcacgagccg
     6361 gtcggagagg aagagcgacg aggcgcccca gaggaccgtc gagggaacga acgcgatccc
     6421 gacctcgagc gggctgtagc cgaggatctg ctggagatag agcgccgaga agaagaactg
     6481 cgcgaacatc ccgcccgcca tgaggacgcc gacgacgttc gccgtcgaga cgttcctgat
     6541 ccggaagatc ctgagcggca cgagcggcgc gggcgtccgg gcctcgatca cgaagaagac
     6601 ggcgaggagc gcgaccgcgg cggcgagcag gccgagcgtc cggccggacg tccagccgac
     6661 ctcgttcccc tgcacgatcg cgtagaccgc gagcatgagc gccgccgtcg cggtcacggc
     6721 gcccgggacg tcgagccgcc gcgaggcgct cgcggcgcgc gcgcccggca ggagccgcag
     6781 cccgagcagg aagacggcga tcccgaccgg gaggttcacg aggaagatcc agtgccagtt
     6841 gaggacgtcg gtcaggacgc cgccgagcag cacgccgatc gtcccgccgc cggagaggac
     6901 gaacccgaag acgcccatcg ccttggcccg gtcggccggc tccgtgaaca ggctcatgac
     6961 gagtgagagc gcgaccgccg agacgaccgc ggcgccgacc ccctgcacgg cgcgcgcgcc
     7021 gacgagcatc ccctgcgtcg tcgcgagccc gcacgcgagc gaggcgagcg tgaagaggac
     7081 gatcccgccg aggaagagcc gccggttgcc gtagaggtcg ccgagccggc cgccgagcag
     7141 caggaacccg ccgaacgtga gcatgtacgc gttcacgacc caggccagcg aggcctgcgt
     7201 gaagccgaga tcgtcgcgga tcgacggcag cgccacgttc acgatcgtcc cgtcgaggac
     7261 gatcatcacg tcgcccaggc agaggacgag gagtgcgagc cagcgggtgc gattctctgc
     7321 tgcggtcatc tcttcctttc gcgtccgtgg ggttctcgca cgttggacgg cggcgccggc
     7381 cggaactcat cggtggcggg ccgatgagtt ctaccggcgc a
//
LOCUS       DASTFC010000102         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_215986,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000102 DASTFC010000000
VERSION     DASTFC010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215986"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFO88_02980"
     CDS             complement(<1..846)
                     /locus_tag="VFO88_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9544518.1"
                     /translation="MDIGFPEALLVVGFLLVAGAGLSGWLHGTVLSISVLSVTVGIAL
                     ALAGVIEADPSATIVLFAVELALVLTLFSDGLFVESELLRRYWGPPALALVVAMPITL
                     VLLALAAKALFPQLSWMEAFLLGAVLSPTDPVVTSAVVTSARIPARVRHTLNLESGLN
                     DGLALPFVLFFLAAAVPGGREPALEALVLAGEALAGGAIGVALALTAGRLLPTLPGGG
                     MTHKYEGSYALGVGFLAFGLAEATWGNGLIAAFVAGIVLAFTRHEIPVTFLEFNESVS
                     AMFQIV"
     gene            complement(853..1266)
                     /locus_tag="VFO88_02985"
     CDS             complement(853..1266)
                     /locus_tag="VFO88_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544519.1"
                     /translation="MRELWMRTLEEALAEDPEADGLASALVVLASVAGREIPLDPDAR
                     HGATRRALLLLAAGGDPARGLDLNGRAVAALADELRDVDRQRALEDGLRDLRQDATGL
                     PHVSEAVRALLAAPDIAWRAYAASLLVEDLEGDDE"
     gene            complement(1283..2215)
                     /locus_tag="VFO88_02990"
     CDS             complement(1283..2215)
                     /locus_tag="VFO88_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544520.1"
                     /translation="MGILYLAREEVAELLPPIPEQLDLVEDVYRALAAGRVELPPKPG
                     VHPRPDSFVHAMPAYLRDEDVVALKWVAGYPANKVRGLPYISGLVVVNDAETGLPTAI
                     LDGAEITAARTAAASGVCVRAFAPAGWREAAILGAGEQGVFHARLLRELNPDVRIRAY
                     DPNPDRVERLGEGVEPAAGELEAVAGAEVVVTAAPIVTEPDAPLGPDALGERWLLLPI
                     DFDALVQRELVEAAGLLAVDDVGQFEYYREQGHFRGWPVPHGSVGESLGRLRAQVPQR
                     VACVNLGIGALDAAFAARVVAAARDRGAGTELPR"
BASE COUNT          333 a          843 c          887 g          256 t
ORIGIN      
        1 gacgatctgg aacatcgcgc tcacgctctc gttgaactcg aggaacgtga ccgggatctc
       61 gtggcgcgtg aacgcgagca cgatcccggc gacgaacgcc gcgatgaggc cgttgcccca
      121 ggtggcctcc gcgagcccga acgcgaggaa cccgacgccg agcgcgtacg acccctcgta
      181 cttgtgcgtc atcccgccgc ccggaagcgt ggggaggagg cggccggccg tgagggcgag
      241 cgcgacgccg atcgcgccgc ccgcgagcgc ctcgccggcg aggacgagcg cctcgagcgc
      301 cggctcgcgc ccgccgggca ccgcggccgc gaggaagaac aggacgaacg ggagcgcgag
      361 cccgtcgttc aaccccgact cgaggttgag ggtgtggcgg acgcgggcgg ggatccgcgc
      421 cgaggtcacg accgcggacg tcacgacggg atccgtcggc gagaggacgg cgccgaggag
      481 gaacgcctcc atccacgaga gctgcgggaa gagcgccttc gccgcgagcg cgaggaggac
      541 gagcgtgatc ggcatcgcga cgacgagcgc gagcgcgggc ggcccccagt agcggcgcag
      601 gagctcgctc tcgacgaaga gcccgtcgga gaagagcgtc agaacgagcg ccagctcgac
      661 ggcgaagagg acgatcgtcg cgctcgggtc ggcctcgatc accccggcga gcgcgagcgc
      721 gatccccacc gtgaccgaga gcacggagat cgacaggacg gttccgtgga gccagccgga
      781 gagccccgcg cccgctacga gcaggaagcc gacgacgagg agcgcctccg ggaacccgat
      841 gtccaccgcg cgttattcgt cgtcgccttc gaggtcctcc acgaggagcg acgccgcgta
      901 ggcgcgccag gcgatgtccg gcgccgcgag gagcgcgcgc acggcctcgc tcacgtgggg
      961 gaggccggtc gcgtcctggc gcaggtcgcg gaggccgtcc tcgagcgcgc gctggcggtc
     1021 gacgtcgcgg agctcgtccg cgagcgccgc cacggcgcgg ccgttcaggt cgagcccacg
     1081 cgccgggtcg cctcccgcgg cgaggagcag gagcgctcgg cgcgtcgcgc cgtggcgggc
     1141 gtcggggtcg agcgggatct cccggccggc gacggacgcg agcacgacga gcgcgctcgc
     1201 gagcccgtcc gcctcgggat cctcggcgag cgcctcctcg agcgtcctca tccacagctc
     1261 ccgcacgcgt ggagtgtgcc cgtcagcgcg gaagctcggt gccggcgccc cggtcgcgcg
     1321 cggcggcgac gacgcgcgcg gcgaaggcgg cgtcgagcgc gccgatcccg aggttcacgc
     1381 aggcgacgcg ctgggggacc tgcgcccgca gacggccgag cgactcgccg acgctcccgt
     1441 gcgggacggg ccagccgcgg aagtggccct gctcccggta gtactcgaac tggccgacgt
     1501 cgtcgacggc gaggaggccg gccgcttcca ccagctcgcg ctggacgagc gcgtcgaagt
     1561 cgatcggcag gaggagccag cgctcgccga gcgcgtccgg cccgagcggc gcgtccggct
     1621 cggtgacgat cggcgccgcg gtgacgacca cctcggcgcc cgcgaccgcc tcgagctcgc
     1681 ccgccgccgg ctcgacgccc tcgccgagcc gctcgacgcg gtccgggttc gggtcgtagg
     1741 cccggatccg cacgtcgggg ttgagctcgc ggaggagccg cgcgtggaag acgccctgct
     1801 cgcccgcgcc gaggatcgcc gcctcgcgcc agcccgccgg cgcgaacgcg cgcacgcaga
     1861 cgccgctcgc cgcagccgtg cgggcggcgg tgatctccgc gccgtcgagg atcgcggtcg
     1921 ggaggcccgt ctccgcgtcg ttcacgacga cgagcccgga gatgtacggc aggccgcgca
     1981 ccttgttcgc cgggtagccg gccacccact tgagcgccac cacgtcctcg tcccggaggt
     2041 aggccggcat cgcgtggacg aacgagtccg gccgcgggtg gacgcctggt ttcggcggga
     2101 gctcgacccg cccggccgcg agcgcgcgat agacgtcctc gacgagatcg agctgctccg
     2161 ggatcggcgg aaggagctcc gcgacctcct cgcgcgcgag gtagaggatc cccacgctag
     2221 gcgacgcgcc gcggctcgat taggaagagg acccgttgct cgcccggacg taccggatac
     2281 tcgtcgtcgg tctcgtggta cttccggttg agcccgtcg
//
LOCUS       DASTFC010000103         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_166605,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000103 DASTFC010000000
VERSION     DASTFC010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166605"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3401)
                     /gene="rpoC"
                     /locus_tag="VFO88_02995"
     CDS             complement(<1..3401)
                     /gene="rpoC"
                     /locus_tag="VFO88_02995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET9544521.1"
                     /translation="MIDINNFDAIRIGLASSKQIRDWSSGEVTKPETINYRTLKPEKD
                     GLFCERIFGPTKDWECYCGKYKRVRYKGIICERCGVEVTRQKVRRERMGHIDLAAPVS
                     HIWFFKGVPSRIGYLLDIAPKELEKVLYFAASIVTEVDKEQRDADVADLEDKVRAESE
                     RIFVDRDEQLAALEGRLQRRRDFFTKGKEKNFDEDDEFWARGLIAWAEEQGLPPLEEA
                     RDLVAGLFKDVAQKITTEDSKKIRELVRSSAIRDDRQLTTRELDQVGTAAVQIREALA
                     PLRAEYEKSSGSKKGAVTRHMTKILDALLAGEELEGDDAELVSGVDAKNLEKARELGN
                     GLLRETIETAEPNASADEVRDLANDLCLKADGRMQKEDLDSLVQWSLKVREMYMDIES
                     RRSDARDAAVDAVNRLEQTWQLFRELEVKQIVSDEMLFRELKDRFGSPYGFGVYFRGG
                     MGAEAIRDLLKDLDLGAEAKSLRETIRTSKGQKQSRAIKRLKVVSAFITSENKPEWMI
                     LEAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEK
                     RMLQEAVDALFDNGRRGRAVTGPGNRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSV
                     IVSGPTLKLHQCGLPKLMALELFKPFIMSRLVERKSVQNIKAAKKYVDSRAPEVWDVL
                     EEVIAEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIQIHPLVCHAFNADFDGDQMAVHL
                     PLSAEAQAEARILMLSANNILSPAHGKPLTTPTQDMVLGGYYLTYCAENLEGMSAEEL
                     DPRPKRLGSETEVVLAYDAKQIELQEPIEFREAGELMLTTPGRVIFNSHVGRALAAAL
                     GDAYDPENRPFVNRTLGKRETNDFISELVDRYGALALSPILDAIKELGFTYATQGGIT
                     VSKNDIVIPEDKEQILGGYEDRVQGIERDYERGLITEEERKERIVEEWTEATDAVADA
                     MQKHMFELNPIYMMANSGARGSIKQIRQLAGMRGLMANPKGDIIERPIKANFMEGLSV
                     LEYFISTHGARKGLADTALRTADSGYLTRRLVDVSQDVIVRDEDCGTDESIELPLLTS
                     DGLNRSLEGRVLAADVAKPLKDGKPGKTVLHPKGELVTSADLRRIA"
     gene            complement(3445..>3747)
                     /locus_tag="VFO88_03000"
     CDS             complement(3445..>3747)
                     /locus_tag="VFO88_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544522.1"
                     /translation="GEVEVREEDDELLRAAEELGIDLSPGSLRATSRQPTAEETEEGQ
                     VRMDEEDVGVPTDEGLTDIDESSPDVVGGESPAEEAEDAAGPFAEAPNLADLEIED"
BASE COUNT          494 a         1291 c         1278 g          684 t
ORIGIN      
        1 gcgatccgcc gcaggtcggc ggacgtgacg agctcgccct tcggatggag caccgtcttg
       61 cccggcttgc cgtccttgag cggcttcgcc acgtcggcgg cgagcacccg gccctcgagc
      121 gagcggttga gcccgtccga ggtgaggagc ggcagctcga tcgactcgtc cgtcccgcag
      181 tcctcgtcgc ggacgatcac gtcctgggag acgtccacga ggcggcgggt caggtagccc
      241 gagtcggccg tgcggagcgc cgtgtcggcg aggcccttgc gggcgccgtg cgtcgagatg
      301 aagtactcga gcaccgacag gccctccatg aagttggcct tgatcgggcg ctcgatgatg
      361 tcgcccttcg gattcgccat gaggccgcgc atcccggcga gctggcggat ctgcttgatc
      421 gatccgcgcg ctcccgagtt ggccatcatg tagatcgggt tcagctcgaa catgtgcttc
      481 tgcatggcgt cggccaccgc gtcggtcgcc tccgtccact cctcgacgat gcgctccttg
      541 cgctcctcct ccgtgatgag gccgcgctcg tagtcgcgct cgatcccctg gacgcgatcc
      601 tcgtagccgc cgaggatctg ctccttgtcc tccgggatga cgatgtcgtt cttcgagacg
      661 gtgatcccgc cctgcgtcgc gtacgtgaag ccgagctcct tgatcgcgtc gaggatcggc
      721 gagagcgcga gggccccgta ccggtcgacg agctcggaga tgaagtcgtt cgtctcacgc
      781 ttgccgagcg tgcggttgac gaacggccgg ttctccgggt cgtacgcgtc gccgagcgcg
      841 gcggcgagcg cccgcccgac gtgggagttg aagatcacgc ggccgggcgt cgtcagcatg
      901 agctcgcccg cctcgcggaa ctcgatcggc tcctggagct cgatctgctt cgcgtcgtac
      961 gcgagcacga cctccgtctc ggagccgagc cgcttcggcc gcgggtcgag ctcctcggcc
     1021 gacatgccct cgaggttctc ggcgcagtag gtcaggtagt agccgccgag gaccatgtcc
     1081 tgcgtcggcg tcgtgagcgg cttcccgtgc gccggcgaca ggatgttgtt cgccgacagc
     1141 atgaggatgc gcgcctccgc ctgcgcctcg gccgagagcg ggaggtggac ggccatctgg
     1201 tcgccgtcga agtccgcgtt gaacgcgtgg cagacgagcg ggtggatctg gatcgccttg
     1261 ccctcgacga gcaccggctc gaatgcctgg atcccgaggc ggtgcagcgt cggcgcccgg
     1321 ttcaggagga ccgggtgctc ggcgatgacc tcctcgagga cgtcccagac ctcgggcgcc
     1381 ctcgagtcga cgtacttctt cgccgccttg atgttctgga cgctcttccg ctcgacgagc
     1441 cggctcatga tgaacggctt gaagagctcg agcgccatga gcttcggcag cccgcactgg
     1501 tggagcttga gcgtcggccc cgacacgatc accgaccggc cggagtagtc gacgcgcttc
     1561 ccgagcaggt tctgccggaa gcggccctgc ttgcccttca gcatgtcgga gagcgacttg
     1621 agcgcgcggt tgcccgggcc cgtcacggcg cggccgcggc gcccgttgtc gaagagcgcg
     1681 tcgacggcct cctggagcat ccgcttctcg ttgttcacga tgatctcggg cgcgccgagg
     1741 tcgagcagcc gcttgaggcg gttgttccgg ttgatcacgc gccggtagag gtcgttcaga
     1801 tcgctcgtgg cgaaccggcc gccgtcgagc tgcaccatcg ggcggagctc cggcgggatg
     1861 accgggacgg cctcgaggat catccactcg ggcttgttct ccgaggtgat gaacgcgctc
     1921 acgaccttga ggcgcttgat cgcgcggctc tgcttctggc ccttcgacgt gcggatcgtc
     1981 tcgcggagcg acttcgcctc ggcgccgagg tcgaggtcct tcagcaggtc gcgaatcgcc
     2041 tccgccccca tgccgccgcg gaagtagacg ccgaacccgt acggcgagcc gaagcggtcc
     2101 ttgagctcgc ggaagagcat ctcgtcggag acgatctgct taacctcgag ctcgcggaag
     2161 agctgccacg tctgctcgag ccggttcacc gcgtcgacgg cggcgtcccg cgcgtccgag
     2221 cggcgcgact cgatgtccat gtacatctcg cgcaccttga gcgaccactg gacgagcgag
     2281 tcgaggtcct ccttctgcat gcggccgtcg gccttcaggc agaggtcgtt cgcgaggtcg
     2341 cggacctcgt cggcggaggc gttcggctcg gccgtctcga tcgtctcgcg caggagcccg
     2401 ttgccgagct cgcgcgcctt ctcgaggttc ttcgcgtcga cgccggagac gagctcggcg
     2461 tcgtcgccct cgagctcctc gcccgcgagg agcgcgtcga ggatcttcgt catgtgccgc
     2521 gtgacggcgc ccttcttcga gccggacgac ttctcgtact cggcccggag cggggcgagc
     2581 gcctctcgga tctgcacggc ggccgtgccg acctggtcga gctcgcgcgt cgtgagctgc
     2641 cggtcgtcgc ggatcgccga cgagcggacg agctcgcgga tcttcttcga gtcctcggtc
     2701 gtgatcttct gcgccacgtc cttgaagagg ccggcgacga ggtcgcgcgc ctcctcgagc
     2761 ggcggcaggc cctgctcctc ggcccatgcg atgaggccgc gggcccagaa ctcgtcgtcc
     2821 tcgtcgaagt tcttctcctt gcccttcgtg aagaagtcgc ggcgccgctg gagccgcccc
     2881 tcgagcgccg cgagctgctc gtcgcggtcg acgaagatgc gctccgactc ggcgcgcacc
     2941 ttgtcctcga ggtcggcgac gtcggcgtcg cgctgctcct tgtccacctc ggtgacgatg
     3001 gacgccgcga agtagagaac cttctcgagc tccttcgggg cgatgtcgag caggtagccg
     3061 atgcggctcg ggacgccctt gaagaaccag atgtgcgaga cgggcgcggc caggtcgatg
     3121 tggcccatgc gctcgcggcg caccttctgg cgggtcacct cgacgccgca gcgctcgcag
     3181 atgatgccct tgtagcggac gcgcttgtac ttgccgcagt agcactccca gtccttcgtc
     3241 ggaccgaaga tgcgctcgca gaagagcccg tccttctccg gcttcagggt ccggtagttg
     3301 atcgtctccg gcttcgtgac ctcgcccgag gaccagtccc ggatctgctt cgacgaggcg
     3361 agcccgatgc gaatcgcgtc aaagttgttg atgtcgatca agcgatcttc ctttcgctag
     3421 ccgtttccgt ggacggaacg gccgttagtc ctcgatctca aggtcggcca ggttcggggc
     3481 ctccgcgaag gggcccgcgg cgtcctcggc ctcctcggcg ggcgactcgc cgccgacgac
     3541 gtccggggac gactcgtcga tgtcggtcag gccctcgtcc gtggggacgc ccacgtcctc
     3601 ctcgtccatc cggacctggc cctcttcggt ctcctcggcg gtcggctggc gcgacgtggc
     3661 gcgcagcgag ccgggcgaga ggtcgatccc gagctcctcc gccgcgcgga gcagctcgtc
     3721 gtcctcctcc cgcacctcga cctcgcc
//
LOCUS       DASTFC010000104         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_743394,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000104 DASTFC010000000
VERSION     DASTFC010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743394"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFO88_03005"
     CDS             complement(<1..299)
                     /locus_tag="VFO88_03005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET9544523.1"
                     /translation="MTPDVVIPTVGRPSLARLLETLAADPPPGRVLVVRDGGPPPPAV
                     DGLAYEVVEGPGRGPAAARNAGWRASGSEWVAVLDDDVVPEPGWAPALADDLRALP"
     gene            complement(296..1930)
                     /locus_tag="VFO88_03010"
     CDS             complement(296..1930)
                     /locus_tag="VFO88_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET9544524.1"
                     /translation="MRVLGINAVFHDPAAALVVDGVTVAAAEEERFSRRKHGKPPVAF
                     STWELPEASARWCLERAGLEPGALDAVAYSYDPDLAPQPNGDVTAENWEPLRTLYTRR
                     APLFLKTALPGLDPEKVRFVPHHVAHAASAHYASPFERSSVMVLDGRGERASYLAARY
                     ENGSHRVLATQELPHSLGLLYEEATAHLGFRRSSDEYKVMALASYGEPVWLDELRRLV
                     VPNGCGGFTVAPIDWNRFCPSRRPDEEWGPEHANLAASVQARLEEMLLALAAWLHGET
                     GDRNLTMAGGVALNCVANSKLWEAGPFDDVWVQPAAGDAGTALGAALQVARELGDEPQ
                     PMPGAALGREWSDGELERRLRTAKVAYERPASIAEAAAEALGADGIVAWFQGRSEYGP
                     RALGHRSLLAHPGRAENLERLNDVKGREQFRPVAPMVLEERAHEIFEGPLPSPYMLFV
                     HRVKPEWRDRIPAVVHVDGTARIQTVSERDEPLVAEMLRAFERRTGLPVVVNTSLNTA
                     GRPMVDDPRDALECFGSAPVDALALGPFLLRRETGG"
     gene            2020..>2216
                     /locus_tag="VFO88_03015"
     CDS             2020..>2216
                     /locus_tag="VFO88_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544525.1"
                     /translation="MASLARTPLAPRRIAILRALMLGDLLCAVPALRAFRRGFPDAEI
                     TLVGLEWARELAPRFSHLVDDV"
BASE COUNT          268 a          851 c          827 g          270 t
ORIGIN      
        1 gggagcgcgc ggagatcgtc cgcgagcgcg ggggcccagc ccggctccgg cacgacgtcg
       61 tcgtcgagga ccgcgaccca ctcggagccc gaggcgcgcc agccggcgtt gcgcgcggcc
      121 gcggggccgc ggcccgggcc ctcgacgacc tcgtacgcga ggccgtccac cgcgggcggg
      181 ggcgggcctc cgtcgcggac gacgagcaca cggcccggcg gcggatcggc ggcgagggtc
      241 tcgagcagcc gcgcgaggga cggccgcccg acggtcggga tgacgacgtc gggcgtcatc
      301 cccccgtttc gcgccgcagg aggaacggtc cgagcgcgag cgcgtcgacc ggcgccgagc
      361 cgaagcactc gagcgcgtcc cgcgggtcgt ccaccatcgg ccgccccgcg gtgttgaggc
      421 tcgtgttcac gacgaccgga aggccggtgc gccgctcgaa cgcgcgcagc atctcggcca
      481 cgagcggctc gtcccgctcc gagacggtct ggatccgcgc cgtcccgtcc acgtgcacga
      541 cggccgggat ccggtcgcgc cactccggct tcacgcggtg gacgaagagc atgtacgggc
      601 tcggcagcgg cccctcgaag atctcgtgcg cccgctcctc gagcaccatc ggcgcgacgg
      661 ggcggaactg ctcccggccc ttgacgtcgt tcagccgctc gaggttctcc gcccggccgg
      721 ggtgcgcgag gagcgagcgg tgcccgagcg cgcgcgggcc gtactcgctg cgcccctgga
      781 accacgcgac gatgccgtcc gcgccgagcg cctccgcggc cgcctcggcg atcgacgccg
      841 gccgctcgta cgcgaccttc gccgtgcgca gccggcgctc gagctcgccg tccgaccact
      901 cgcgcccgag cgcggcgccc ggcatcggct gcggctcgtc gccgagctcg cgcgcgacct
      961 ggagcgccgc gccgagcgcc gtccccgcgt cgccggcggc gggctgcacc cagacgtcgt
     1021 cgaacggccc ggcctcccag agcttcgagt tcgcgacgca gttcagcgcc acgccgcccg
     1081 ccatcgtgag gttccggtcg ccggtctcgc cgtgcagcca ggccgcgagc gcgaggagca
     1141 tctcctcgag ccgcgcctgg acgctcgccg cgaggttcgc gtgctctggc ccccactcct
     1201 cgtcggggcg gcgcgacggg cagaagcggt tccagtcgat gggcgcgacc gtgaacccgc
     1261 cgcagccgtt cggcacgacg agccggcgca gctcgtccag ccagacgggc tcgccgtacg
     1321 aggcgagcgc catcaccttg tactcgtccg acgagcggcg gaagccgagg tgcgcggtcg
     1381 cctcctcgta gagcagcccg agcgagtgcg gcagctcctg cgtcgcgaga acgcggtgcg
     1441 agccgttctc gtagcgcgcg gcgaggtagg acgcgcgctc gccgcggccg tcgagcacca
     1501 tcacgctcga gcgctcgaac ggcgaggcgt agtgcgccga ggcggcgtgc gcgacgtggt
     1561 gcgggacgaa gcgcaccttc tccgggtcga gccccggcag cgcggtcttc aggaagagcg
     1621 gcgcgcggcg cgtgtagagc gtgcggagcg gctcccagtt ctcggcggtc acgtcgccgt
     1681 tcggctgggg cgcgaggtcg gggtcgtacg agtacgcgac ggcgtcgaga gcccccggct
     1741 cgaggccggc gcgctcgagg caccagcgcg cggaggcctc cggcagctcc cacgtcgaga
     1801 acgcgacggg cggcttcccg tgcttgcgcc ggctgaagcg ctcctcctcg gcggcggcga
     1861 cggtgacacc gtccacgacg agcgcggcgg ccgggtcgtg gaagacggcg ttgatcccga
     1921 ggacccgcat cgcgccgcct ctttccactt ctcccgcccg gaaacatcgc gcgcgcgggt
     1981 gtaactcgac ggcgaggcgg gcaccggccg gagaaccgga tggcctcgct cgcgcgcacc
     2041 ccgctcgccc cgcgccggat cgcgatcctc cgcgcgctca tgctcggcga cctgctctgc
     2101 gccgtgccgg ccctgcgcgc gttccgccgc gggttcccgg acgccgagat cacgctcgtc
     2161 gggctcgaat gggcccggga gctcgcgcct cgcttctcgc acctcgtgga cgacgt
//
LOCUS       DASTFC010000105        15007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1105962,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000105 DASTFC010000000
VERSION     DASTFC010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105962"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFO88_03020"
     CDS             complement(<1..419)
                     /locus_tag="VFO88_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9544526.1"
                     /translation="MRRGLLLLLSVCAALAAAAPVSAQTEAPEPPDVAAPAFLVADAR
                     TGEILAERNADARRQIASITKLMTALVVLDHARPGETVTVLPRASSIGESTIDLVPGE
                     RMSVRDLLAAALIQSANDAAFALAAHVGDGSVPRFVEL"
     gene            454..774
                     /locus_tag="VFO88_03025"
     CDS             454..774
                     /locus_tag="VFO88_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544527.1"
                     /translation="MAEEKAVVRRHGVERSLEIQLGPLGGIDVRPTRVFVWGGEEREG
                     RERIVPLGSPPRNLRIPRRERELQAGAVHVRSLGNPLMTIALAGVHVARRLVAEREDE
                     RRAT"
     gene            complement(767..1159)
                     /locus_tag="VFO88_03030"
     CDS             complement(767..1159)
                     /locus_tag="VFO88_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9544528.1"
                     /translation="MREQTDERIERGEPTRRPRQPWGARILAPLAFFAAATALVLVVN
                     RALDGGSEAATTTPPPAAVATETSATGTATDGENVPRRERQFHRIRPGDTLEAVANRY
                     ETTVDDLLRLNPNIEPNNLSPGQRIRVR"
     gene            complement(1159..2088)
                     /gene="pyrF"
                     /locus_tag="VFO88_03035"
     CDS             complement(1159..2088)
                     /gene="pyrF"
                     /locus_tag="VFO88_03035"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9544529.1"
                     /translation="MEQELAAVPQTAVVPFADRVAEAVERKRSQLVLGVDPRPELLPL
                     ELRAAAHAGRAAAAEACARFSCGLVDAAGPYVVGVKLQLAFFEALGADGLAAFEEACA
                     YARAAGLLVIADGKRGDIGSTARAYADAYVQATEAGAPRVDAITVNPYLGGDSLEPFL
                     AAARRSGAGLFVLVKTSNGGSADVQELTLSDGRPVWHQVAELVADWGEELVGERGLSS
                     VGAVVGATYPRAVGEARRLMPQAILLLPGIGAQGATPADVARAFTSGPASALVPVSRA
                     VIYAFRVEGADWRTAAAAEAARLKREVWTASGW"
     gene            complement(2094..3788)
                     /locus_tag="VFO88_03040"
     CDS             complement(2094..3788)
                     /locus_tag="VFO88_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HET9544530.1"
                     /translation="MQLRLDAADRLVELVEERRAPVPATEAATALFALARPAAGLAAT
                     LLAEVVESDARLAWSGSAVALAGRGPESLPLEAATFVVFDLETTGLSARTAEICEIGA
                     VRIRGLEPAGTFETLVRPGAPLPSAVAALTGIRDAELRRAPPLGAAVRRFLAFAGDCV
                     LVAHNARFDVGFLDRAVERLDGRRSAAPVLDTVGLARTLLAGRVRRHGLASLAAWLGT
                     SVEPCHRALPDAQATAEILVHLIGLAQERGAETVADLQRLAAPRPRRAHDKRSLAFGA
                     PTLPGIYLFRGAGDQVLYVGKARDLRARLRSYFGSGRQRPAVEAALSALERIEWRVLG
                     SELEAALEELRLIRELRPPGNARGGRPDRYRYLELRGDRVAVTDEPGPLGPIRSRRRA
                     ELAARALAGATPEELDGLAAGLPLARVRARLARLAEAQRYEDAARMRDRLRALEAVAG
                     GLAALDRLRRAELCLLVPARERGFRQAYAVAGGRIAASRRLPLHGGAIVEAAALVAEA
                     GAARRAGTTHSPEAADELLLVSSFLRRPPPELDVCELDAARIAAATLGYRADADVA"
     gene            3848..4156
                     /locus_tag="VFO88_03045"
     CDS             3848..4156
                     /locus_tag="VFO88_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9544531.1"
                     /translation="MMMFHVVIHRSGPAWQPGLPLEEQSGWEEHAAFMDGLVDAGVIV
                     LGGPLADEFRVVHAIEAESEEAVRAALAPDPWAGTHLEIAAVEPWTIRLDGRRREGRP
                     "
     gene            complement(4196..4480)
                     /locus_tag="VFO88_03050"
     CDS             complement(4196..4480)
                     /locus_tag="VFO88_03050"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9544532.1"
                     /translation="MAVTKSEFVDRVASKSGLSKRDAGKAVDAFLDSVQETLVQGDSV
                     TFTGFGKFSAQQRNERQGVNPRTGERVTIAAAKVPKFSAGSQLKSAVKGS"
     gene            complement(4579..5109)
                     /gene="pyrE"
                     /locus_tag="VFO88_03055"
     CDS             complement(4579..5109)
                     /gene="pyrE"
                     /locus_tag="VFO88_03055"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET9544533.1"
                     /translation="MNDGELAALLKERAWLEGDFVLRSGRRSSYYLDKYRFETHPALL
                     RELGERIAAQARNGGDPPDLLAGPELGAVALAASASMASGLPFLIVRKEQKDYGTSNR
                     IEGVFEAGQRVCLVEDVVTTGGAAVAAVEALRDAGLECRRAICVVDRDEGGADALARA
                     AVRLHPLFRTRDLQTA"
     gene            complement(5106..5303)
                     /locus_tag="VFO88_03060"
     CDS             complement(5106..5303)
                     /locus_tag="VFO88_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544534.1"
                     /translation="MRRLLALGVAGAAAGIALWRRRGGAPAERVDLHFEDGSMVSLPA
                     VSEDAERLLVLARDVLAAARS"
     gene            complement(5296..5724)
                     /locus_tag="VFO88_03065"
     CDS             complement(5296..5724)
                     /locus_tag="VFO88_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9544535.1"
                     /translation="MAAADAAQALEELVEISSQIEGAVLLAEDGALVASTYPDAGRAE
                     RVAAAASELLRAADGSQARAERAAIGQLVVELPDGAVVCVREDGRAVAAVTVKEPTVG
                     LVLYDLRTTLRLAADEKPKRKPAARKKAAASDTAGEAADA"
     gene            5910..6722
                     /locus_tag="VFO88_03070"
     CDS             5910..6722
                     /locus_tag="VFO88_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9544536.1"
                     /translation="MHQPPVLGKFLADRGTHLAAMIAYFATLAFVPLLFLALSLIAVF
                     GQADESSYLIEQLRRAFPAASVENLVSVVEEIQASAAGLGIVGGVALVWAALGFFSVV
                     ESALNIVYGLPNRPFIRQKLFVLLLTAAALALLFLALFVGSVGVGIARHIGFGGTVAY
                     LYGIAISSVLVLVFVWSIYTLLTNERLRWRETLPGALFATLVLQASFQLLPLFVRATS
                     GLIALQAFGGFALLLLWIYLMANVIVLGAEVNWHLSRGRVEARREAETEGLA"
     gene            complement(6719..7168)
                     /locus_tag="VFO88_03075"
     CDS             complement(6719..7168)
                     /locus_tag="VFO88_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9544537.1"
                     /translation="MLFPHDTKVERLREAPLFADLSKDELEQLAKLTEDLEVEAGKVL
                     CREGEPAQEFFVIIEGTAEATKDGRQLGTMGQGDFFGEIALIEDIPRTATVTATSHLR
                     FFVLTRQAFWSIVDRMPDVERKVLRTLARRVVENWGDPTTANRPEPS"
     gene            complement(7178..7768)
                     /locus_tag="VFO88_03080"
     CDS             complement(7178..7768)
                     /locus_tag="VFO88_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HET9544538.1"
                     /translation="MRLVLVSENPHKARELAAALPGWQVEPWTGPPLPPETGATFREN
                     ARGKAAFGREHGPPGAWVAGEDSGLEVTGLGGRPGIFSARYAGERASDDANTAKLLEE
                     LDGLEPWSRAARYVCALVALSPDGEELAVEGTLEGAIAEERRGSAGFGYDPVFVPDGE
                     TRTVAELGDAWKAAWSHRARAARALAAALGERTPTV"
     gene            complement(7765..8472)
                     /locus_tag="VFO88_03085"
     CDS             complement(7765..8472)
                     /locus_tag="VFO88_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET9544539.1"
                     /translation="MQQTQQLIPELELWELALRLGVAAALTGAIGLERELRERAAGLR
                     THMLVGVGAALFTIVSAYGWSDFVFNRDQGTILDPTRIAAQIVTGIGFLGAGAIIRQG
                     LSVRGLTTAAGLWVVAAIGMAAGAGYYTAALIGTGIVLVGLGPIRWLEGAPLIRDLRR
                     EGRALEVLLHPGESVGGVLDVLHDRTVRISRVEISDLEEGRQVRVEIDLPLGGAGSEL
                     VENLARREDVIAVRWGA"
     gene            complement(8444..9157)
                     /gene="rph"
                     /locus_tag="VFO88_03090"
     CDS             complement(8444..9157)
                     /gene="rph"
                     /locus_tag="VFO88_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET9544540.1"
                     /translation="MTRADGRRPDELRPLEIAPGFLEQADGSALISLGRTRVLCTATV
                     EDGVPRWLEGKGQGWLTAEYGMLPASTGERMRREASAGRQKGRTVEIQRLIGRSIRGA
                     YDLANLGERTVWLDCDVLQADGGTRTAAVTGAWVALTLAARARGFTEPPEAIAAVSLG
                     IVDGEFLLDLDYREDSSAEVDMNIVMTGDGRIIDIQATAERGPFPHETLDALVALARG
                     GIDRIAEAQRRATDAAAHS"
     gene            complement(9154..9972)
                     /gene="murI"
                     /locus_tag="VFO88_03095"
     CDS             complement(9154..9972)
                     /gene="murI"
                     /locus_tag="VFO88_03095"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9544541.1"
                     /translation="MDSRPIGIFDSGAGGLTVLHECLVTLPHEDFVYLGDGERCPYGP
                     REAEEIRRFALELAAYLERSGVKLVVAACNTAAAAALPYLQERLDVPVVAVLAPEAHA
                     AVQATRNRRVGVLATEATIESGRYADVIHTLDAGVEVVSVACPKLVPLIEAGGPNEEL
                     LAAVREYAAPLRDAQVDTVVLGCTHYPLVRPVFERVFGRGTTLVFSADETAREVAETL
                     HRKGVGNDPVRHGETTFLTTGSPAEFAALGERFLQLPVAGARRVALDELALEAA"
     gene            complement(10064..11311)
                     /locus_tag="VFO88_03100"
     CDS             complement(10064..11311)
                     /locus_tag="VFO88_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9544542.1"
                     /translation="MSPTPRAAVLVGAAALSALLVPLPVAALLAVAVLAATVVDALLV
                     RRPPVVRRSVPATLSRGVAAPLRLELEEPPPGAARLRQPVPPDLRLEPSEADGRLDGV
                     LLPRRRGRHVLPPAAVRLTGPLGLGAWTRRAGEAAEALVYPDLPAAFRLALAVRQGRF
                     RDTGRATRGPLGLGTEFESVRDYLPDDDVRQVNWRATARLGRPMSNQYRIEQDRDVVC
                     LVDAGRLMAAPLGDRTRLDAAVDAAAAVAAVADELGDRCGAIAFDAELRRNVAPRRGG
                     GRSVIGALFDVEPERVDSDYELAFRAVGSSKRALVIVFTDLLEEAAARPLVEAIHVLR
                     RRHAVAVASAADTDLDRLVRRAPETALDAYAATVAVEVLDARARVAARIRATGADVIE
                     APPDGLGAACVGAYLRAKARAAL"
     gene            complement(11308..12237)
                     /locus_tag="VFO88_03105"
     CDS             complement(11308..12237)
                     /locus_tag="VFO88_03105"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9544543.1"
                     /translation="MRELRDRVRAEVGKVLVGQDEAVELMLAALVCRGHVLLEGVPGV
                     AKTLAANAVARALGLEYRRVQFTPDMLPSDLTGTMTLKGGELVFRAGPVFTQLLLADE
                     INRTPPKTQAALLEAMQEAQVTIEGEPHPLPDPFLVVATQNPIEYEGTYPLPEAQLDR
                     FLFKVDVGYPSEEDEHAVLRLRRRGVVPATLEDVAGVVDAGALADARGTLDATTVSDE
                     VAAYVVALVRATRDLPSVTLGASPRAAVHLVTAARAAARLDGREFVTPDDVVRMAPAV
                     LRHRLVLRPEAELERYRPDDAVQAALAAVPVPR"
     gene            complement(12234..13346)
                     /locus_tag="VFO88_03110"
     CDS             complement(12234..13346)
                     /locus_tag="VFO88_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HET9544544.1"
                     /translation="MRRPARARTVVALVVGAVVAVNLLLAALAELTRVPAGPPSSSYA
                     TAPEGVAAYADLLARSGHPVERLRDPLGEGELDPAVTLVLLDPGPLDGDDRAALARFV
                     EDGGHLLGGGAAPGRWVAELLPEPPAWAPTGPRRTEVVVPTDDVRGVETVETAGRGVW
                     EDAGEALPVLAGGRQAVVVVARRGRGTMTLLADPSILQNRLLARADNAAFGLAAAGEP
                     RRPVVFVESVHGYGRGSGLGAIPSRWAWALAGLGLAALLFMWARGRRLGPAEDAERDL
                     PPPRLEYVESLAALLARSRRPGEAAAPLQAAVRRALARRAGLPPGASDDDVRAAAARF
                     GLPEDEREAALGPVRDAAGLAAAGRALARLSGSGRA"
     gene            complement(13343..14188)
                     /locus_tag="VFO88_03115"
     CDS             complement(13343..14188)
                     /locus_tag="VFO88_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HET9544545.1"
                     /translation="MSARLAFVAVSVAALLTAAPAGAQEVTGGELRDLAARAQTDPAA
                     LEELRDVERVDGAPADLELALEGAGGEELAGRLEALADGAEASPGAAPPARAQDDARA
                     ILSERRFREPDVPRPFRRPLAWLGERIDALAGRVPGGPPVFWVLLGAVVVALAALAAA
                     RVGRRRAADAVERGRAASARGEVDVRRLEEEAAEAERSGDLERAVRLLFRAGLVRLAR
                     ARAIVLRDSLTTGQVRRELRSPDFDGVARVFDEVAYGRRPPEPADVEASRAGWRRVLA
                     EVGTR"
     gene            complement(14185..>15007)
                     /locus_tag="VFO88_03120"
     CDS             complement(14185..>15007)
                     /locus_tag="VFO88_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544546.1"
                     /translation="DAEFAAWFAAILVGALLGFVATTLATGACYKAVADAYLGETPNW
                     RTSLGYALRRLHSLLWISLIYAFTVGLGLLLCLLPGIWIAVAWVVAIPVLLTEGRKGL
                     KAVGRSFRLTRNRWWPAFGLMLVGVMIAGFLSYAVQFLAFAPAFTDAGDSAVLVLTAN
                     AVGQTLAAALWTPLIAAFATVLYFDLRVRKEGFDLALLAERIGVAPDPDRVSLAPRAA
                     PSPSSGEQPPYWPPPPGWRPSSEEGPPPGGETSPEPERPPYWPPPPGWKPGGRDE"
BASE COUNT         1859 a         5725 c         5730 g         1693 t
ORIGIN      
        1 agctcgacga accgcgggac gctgccgtcg cccacgtgcg cggcgagcgc gaaggcggcg
       61 tcgttcgcgc tctggatgag cgcggccgcc aggaggtcgc ggacgctcat ccgctcgccg
      121 gggacgaggt cgatcgtcga ctcgccgatc gagctcgcgc gcgggaggac ggtcaccgtc
      181 tcgcccggcc gcgcgtggtc gaggacgacg agcgccgtca tgagcttcgt gatgctcgcg
      241 atctggcggc gggcgtccgc gttccgctcg gcgaggatct cgcccgtcct cgcgtccgcc
      301 acgaggaacg cgggtgcggc gacgtcgggc ggctcgggcg cctccgtctg cgcggacacg
      361 ggtgcggcgg cggcgagcgc ggcgcagacg gagagcagga gcagcaggcc acgccgcacg
      421 gggcctcact gtaccgtcac tagaattcgc ctcgtggcag aggaaaaggc tgtggtgcgg
      481 cgacacggcg tggagcgctc gctcgagatc cagctcgggc cgctcggggg gatcgacgtg
      541 cggccgacgc gcgtgttcgt ctggggcggc gaggagcggg agggccgcga gcgcatcgtc
      601 ccgctcggaa gcccgccgag gaacctccgc atcccgcgac gcgagcgcga gctccaggcg
      661 ggggcggtgc acgtgcgctc gctcgggaac ccgctcatga ccatcgccct cgcgggcgtg
      721 cacgtcgcac gccggctcgt cgccgagcgc gaggacgagc gccgcgctac ctgacgcgga
      781 tccgctggcc gggcgagagg ttgttcggct cgatgttcgg gttgagccgc aggaggtcgt
      841 ccacggtcgt ctcgtagcgg ttcgcgaccg cctcgagcgt gtcgccgggc cggatccggt
      901 ggaactgccg ctcgcgccgc gggacgttct cgccgtccgt ggcggtgccg gtcgcgctcg
      961 tctcggtcgc gacggccgcc ggcggcgggg tcgtcgtggc cgcctcggag cctccgtcga
     1021 gcgcgcggtt cacgacgagc acgagcgccg tcgcggcggc gaagaaggcg agcggcgcga
     1081 ggatccgcgc gccccacggc tggcgcggcc gccgcgtggg ctcgcctcgc tcgatccgct
     1141 cgtccgtctg ctccctcatc accagcccga ggccgtccag acctcgcgtt tgagccgcgc
     1201 cgcctccgcg gccgcggcgg tccgccagtc ggcgccctcc acgcggaagg cgtagatgac
     1261 ggcgcgcgag acgggcacga gggcgctcgc cggcccgctc gtgaacgcgc gcgccacgtc
     1321 ggcgggcgtc gcgccctggg cgccgattcc cggcaggagc aggatcgcct gcggcatgag
     1381 ccgccgcgcc tcgccgaccg cgcgggggta cgtcgcgccg acgaccgcgc cgacgctcga
     1441 gagcccgcgc tcgccgacga gctcctcgcc ccagtcggcc acgagctcgg ccacctggtg
     1501 ccagaccggc cgcccgtccg agagggtgag ctcctgcacg tccgcgctgc cgccgttcga
     1561 cgtcttcacg agcacgaaga gcccggcgcc cgagcgccgc gcggcggcga ggaagggctc
     1621 gagcgagtcg ccgccgaggt acgggttcac ggtgatcgcg tccacgcggg gcgcgccggc
     1681 ctccgtcgcc tggacgtagg cgtccgcgta cgcgcgcgcg gtcgagccga tgtcgccgcg
     1741 cttcccgtcg gcgatcacga gcagcccggc ggcgcgcgcg taggcgcagg cctcctcgaa
     1801 cgcggcgagc ccgtccgcgc cgagcgcctc gaagaacgcg agctggagct tcacgccgac
     1861 gacgtacggg ccggccgcgt ccacgagccc gcaggagaag cgcgcgcacg cctcggccgc
     1921 cgccgcgcgg cccgcgtggg cggcggcgcg gagctcgagc ggcaggagct ccgggcgcgg
     1981 gtcgaccccg agcacgagct ggctccgctt ccgctcgacc gcctcggcga cgcggtcggc
     2041 gaagggcacg accgccgtct gcgggactgc cgcgagctcc tgctccatgg ctcttaggct
     2101 acatcggcgt cggcccgata tccgagggtc gcggcggcga tccgtgctgc gtcgagctcg
     2161 cagacgtcga gctccggagg cgggcggcgc aggaaggacg agacgagcag gagctcgtcc
     2221 gcggcctccg ggctgtgcgt cgtccccgcg cggcgggcgg ccccggcctc ggcgacgagc
     2281 gccgccgcct cgacgatcgc gccgccgtgg agcgggagcc ggcgggaggc ggcgatccgc
     2341 ccgcccgcga ccgcgtacgc ctgccggaag ccccgctcgc gcgccgggac gaggaggcag
     2401 agctcggcgc gccggagccg gtcgagcgcg gcgagcccgc cggcgaccgc ctcgagcgcc
     2461 cggagccggt cgcgcatgcg ggcggcgtcc tcgtagcgct gcgcctcggc cagccgggcg
     2521 agcctcgcgc ggacgcgtgc gagcgggagg cccgcggcga gtccgtcgag ctcctccggc
     2581 gtcgcgcccg cgagcgcgcg ggcggcgagc tcggcccggc ggcggctgcg gatcggcccg
     2641 agcggccccg gctcgtcggt gacggcgacc cggtcgccgc gcagctcgag gtagcggtag
     2701 cggtccggcc gcccgccccg ggcgttcccg ggcgggcgga gctcgcggat gaggcgcagc
     2761 tcctcgagcg ccgcctcgag ctcggagccg agcacccgcc actcgatccg ctcgagcgcc
     2821 gagagcgccg cctcgacggc gggccgctgg cgcccgctcc cgaagtacga gcgaagccgc
     2881 gcgcggaggt cgcgcgcctt gccgacgtag agcacctggt cgccggcccc gcggaagagg
     2941 tagatgccgg ggagcgtcgg cgcgccgaac gcgagggagc gcttgtcgtg cgcccggcgc
     3001 gggcgcggcg cggcaaggcg ctggaggtcg gcgaccgtct cggcgccgcg ctcctgcgcg
     3061 aggccgatca ggtgcacgag gatctcggcc gtcgcctgcg cgtcggggag ggcgcggtga
     3121 cacggctcga ccgacgtccc gagccaggcg gcgagggagg cgagaccgtg gcggcgcacg
     3181 cggccggcga ggagcgtccg cgcgagcccg accgtgtcga ggacgggcgc ggccgaccgc
     3241 cggccgtcga gccgctcgac cgcgcggtcg aggaacccga cgtcgaagcg cgcgttgtgc
     3301 gccacgagga cgcagtcacc ggcgaaggcg aggaagcggc ggacggccgc gccgagcggc
     3361 ggcgcacggc ggagctcggc gtcgcggatc ccggtgagcg cggcgacggc ggacgggagc
     3421 ggcgccccgg gtcgcacgag cgtctcgaac gtccccgccg gctcgagccc gcggatgcgg
     3481 acggccccga tctcgcagat ctccgccgtc cgcgccgaga gcccggtcgt ctcgaggtcg
     3541 aagacgacga aggtcgccgc ctcgagcggg aggctctccg gcccgcgccc ggcgagcgcg
     3601 accgcggagc cggaccaggc gaggcgcgcg tccgactcga cgacctccgc gaggagcgtg
     3661 gccgcgagcc ccgcggcggg ccgcgcgagc gcgaagagcg ccgtcgcggc ctcggtcgcg
     3721 ggcacgggcg cacggcgctc ctccacgagc tccacgagcc ggtccgctgc gtcgaggcgt
     3781 agttgcacgg gccgccggag gatagaacgt atgttcggac gacacgcgcc gctgctacaa
     3841 cgcccgcatg atgatgttcc acgtcgtgat ccaccgctcg gggccggcct ggcagcccgg
     3901 cctcccgctc gaggagcagt ccggctggga ggagcacgcg gcgttcatgg acggcctcgt
     3961 cgacgccggc gtgatcgtgc tcggcggccc gctcgcggac gagttccggg tcgtccacgc
     4021 gatcgaggcc gagtccgagg aggccgtccg cgcggcgctc gcgcccgacc cgtgggccgg
     4081 cacgcacctc gagatcgccg ccgtcgagcc gtggacgatc cggctggacg gcagacggag
     4141 ggagggccgc ccgtagcggg cgaccctccg aagtcggaaa agaggggcgg ttcttctagg
     4201 agcccttgac ggcgctcttg agctggctgc cggcagagaa cttcggcacc ttcgcggcgg
     4261 cgatggtgac ccgctcgccg gtgcgggggt tgacgccctg gcgctcgttg cgctgctggg
     4321 cggagaactt gccgaacccg gtgaacgtga cggagtcgcc ctgcacgagc gtctcctgaa
     4381 cggagtcgag gaacgcgtcg acggccttgc cggcgtcccg cttcgagagc ccgctcttcg
     4441 acgcaacgcg atccacgaac tcagacttgg tgactgccac tgccctctcc tccctactgg
     4501 tcggtactcg gacgaactgg cacggaaccg taacaggtgc gtcggaaatc ccgcaaccaa
     4561 gcggcctgga gggatcttct aggccgtctg gaggtcgcgc gtgcggaaga gcggatggag
     4621 gcggaccgcg gcccgcgcga gggcgtcggc gcctccctcg tcgcggtcga cgacgcagat
     4681 cgcgcgacgg cactcgaggc cggcgtcgcg cagcgcctcg acggccgcga cggccgcgcc
     4741 gccggtcgtc acgacgtcct cgacgaggca cacacgctgc cccgcctcga agacgccctc
     4801 gatccggttc gaggtgccgt agtccttctg ctccttgcgc acgatgagga acgggagccc
     4861 ggacgccatc gacgcggagg cggccagcgc gaccgcgccg agctccggcc ccgcgaggag
     4921 gtcgggcggg tcgccgccgt tgcgcgcctg cgccgcgatc cgctccccga gctcgcgcag
     4981 gagcgccgga tgcgtctcga agcggtactt gtccaggtag tacgaggagc gccggccgga
     5041 gcggaggacg aagtcgccct cgagccacgc ccgctccttc aggagggcgg cgagctcccc
     5101 gtcgttcacg agcgggccgc ggcgagcacg tcgcgggcga gcaccaggag ccgctcggcg
     5161 tcctcggaca ccgcgggcag cgagaccatc gagccgtcct cgaagtggag gtcgacgcgc
     5221 tctgccggcg caccaccgcg cctgcgccag agcgcgatcc ccgccgcggc gccggcgacg
     5281 ccgagcgcga ggagcctacg catcggccgc ctccccggcg gtgtcggacg cggcggcctt
     5341 ctttcgcgcg gccggcttgc gcttcggctt ctcgtccgcc gccaggcgga gggtcgtgcg
     5401 gaggtcgtag aggacgaggc cgaccgtcgg ctccttgacg gtgacggcgg cgacggcgcg
     5461 cccgtcctcg cgcacgcaga cgacggcccc gtcggggagc tcgacgacga gctggccgat
     5521 cgccgcgcgc tcggcgcggg cctgtgaccc gtcggcggcg cggaggagct cggacgccgc
     5581 ggcggcgacg cgctccgccc gtccggcgtc gggatacgtc gacgcgacga gcgccccgtc
     5641 ctcggcgagg aggacggcgc cctcgatctg cgaggagatc tcgacgagct cctcgagcgc
     5701 ctgagcagcg tccgcggcgg ccacgcgcgc aagctaccac ggcgcccgca ctctctcgcc
     5761 cacgccccgc ccccgggggc gggtccggtc ccctgcgaac gagcctagcg gcgacggacg
     5821 cacgggtccg tgccggaacc gtgccgatcc tgcgcggcgg ccgccggtcg gccacggcca
     5881 ccggcggggc ggcgtcctag actcgccgga tgcaccagcc gcccgtgctc gggaagttcc
     5941 tcgccgaccg cggaacgcac ctcgcggcga tgatcgcgta cttcgcgacg ctcgcgttcg
     6001 tgccgctcct cttcctcgcg ctctcgctca tcgccgtgtt cggccaggcg gacgagtcga
     6061 gctacctgat cgagcagctc cgccgtgcgt tcccggccgc gtcggtcgag aacctcgtga
     6121 gcgtcgtcga ggagatccag gcgagcgccg ccgggctcgg gatcgtcggc ggcgtcgcgc
     6181 tcgtctgggc ggccctcggg ttcttcagcg tcgtcgagag cgcgctcaac atcgtctacg
     6241 ggctcccgaa ccggccgttc atccggcaga agctcttcgt cctccttctc accgcggccg
     6301 cgctcgcgct cctcttcctc gccctcttcg tcggctcggt cggggtgggg atcgcgcgcc
     6361 acatcggctt cggcggcacg gtcgcgtacc tctacgggat cgcgatctcg agcgtcctcg
     6421 tcctcgtctt cgtctggagc atctacacgc tcctcacgaa cgagcggctc cgctggcgcg
     6481 agacgctgcc cggcgcgctc ttcgccacgc tcgtgctcca ggcgagcttc cagctcctcc
     6541 cgctcttcgt ccgcgcgacg agcggcctca tcgcgctcca ggccttcggc ggcttcgcgc
     6601 tgctcctgct ctggatctac ctgatggcga acgtgatcgt gctcggcgcg gaggtcaact
     6661 ggcacctgtc gcgcgggcgc gtcgaggcgc gccgggaagc cgagaccgag gggctcgcct
     6721 aggagggctc gggccggttc gcggtcgtgg ggtcgcccca gttctcgacg acccggcggg
     6781 cgagggtgcg gagaaccttc cgctcgacgt ccggcatgcg gtcgacgatg ctccagaagg
     6841 cctgccgcgt gaggacgaag aaccgcagat ggctcgtcgc cgtgacggtc gccgtccgcg
     6901 ggatgtcctc gatgagcgcg atctcgccga agaagtcgcc ctggcccatc gtcccgagct
     6961 gtcgtccgtc cttcgtcgcc tccgccgtgc cctcgatgat gacgaagaac tcctgcgccg
     7021 gctcgccctc gcggcagagc accttgcccg cctcgacctc gaggtcctcg gtcagcttgg
     7081 cgagctgctc gagctcgtcc ttcgagaggt cggcgaagag cggagcctcg cggagccgtt
     7141 cgaccttcgt gtcgtgcgga aagagcatgc gggcaatcta caccgtgggc gtgcgctcgc
     7201 ctaacgccgc ggcgagggcg cgcgccgccc gggcgcggtg gctccaggcc gccttccacg
     7261 cgtcaccgag ctccgcgacc gtgcgcgtct cgccgtcggg gacgaagaca gggtcgtagc
     7321 cgaagcccgc gctgccgcgg cgctcctcgg cgatcgcgcc ctcgagcgtg ccctcaaccg
     7381 cgagctcctc gccgtcggga gagagcgcga cgagcgcgca gacgtagcgg gccgcgcggg
     7441 accacggctc gagcccgtcg agctcctcga gcagcttcgc ggtgttcgcg tcgtcgctcg
     7501 cgcgctcgcc ggcgtagcgg gccgagaaga tcccgggccg cccgccgagc ccggtcacct
     7561 cgagcccgga gtcctcgccc gcgacccacg cgcccggcgg gccgtgctcg cgcccgaacg
     7621 ccgccttgcc gcgcgcgttc tcgcggaacg tcgcgcccgt ctcgggcgga agcggcgggc
     7681 ccgtccacgg ctccacctgc cagccgggga gggcggcggc gagctcccgc gccttgtgcg
     7741 ggttctcgga gacgaggacg agcctcacgc gccccagcgg acggcgatga cgtcctcgcg
     7801 gcgcgcgagg ttctcgacga gctccgagcc ggcgccgccc agggggaggt cgatctcgac
     7861 gcgcacctgg cggccctcct cgaggtcgga gatctcgacg cggctgatcc ggacggtgcg
     7921 atcgtggagc acgtcgagca cgccgccgac cgactcgccc gggtggagca ggacctcgag
     7981 cgcccgtccc tcccgacgga ggtcgcggat gagcggcgcc ccctcgagcc accggatcgg
     8041 cccgaggccg acgaggacga tgcccgtccc gatgagcgcc gcggtgtagt acccggcccc
     8101 cgcggccatg ccgatcgcgg cgacgaccca gaggccggcc gccgtcgtca gcccgcgcac
     8161 ggagagcccc tggcggatga tcgcgcccgc gccgaggaag ccgatcccgg tcacgatctg
     8221 cgcggcgatc cgggtcgggt cgagaatcgt cccctggtcg cggttgaaga cgaagtccga
     8281 ccagccgtac gcggagacga tcgtgaagag cgccgcgccg accccgacga gcatgtgcgt
     8341 gcgcaggccc gcggcccgct cccggagctc ccgctcgagg ccgatcgcgc cggtcagcgc
     8401 ggcggcgacg ccgagccgga gggcgagctc ccagagctcg agctcaggaa tgagctgctg
     8461 cgtctgttgc acggcgctgg gcctccgcga tccggtcgat ccctccgcgg gcgagcgcga
     8521 cgagcgcgtc gagcgtctcg tgcgggaacg ggccccgctc cgcggtcgcc tggatgtcga
     8581 tgatccgtcc gtcccccgtc atgacgatgt tcatgtccac ctcggccgac gagtcctcgc
     8641 ggtagtcgag gtcgaggagg aactcgccgt cgacgatgcc gagcgagact gccgcgatcg
     8701 cctcgggcgg ctcggtgaac ccgcgcgcgc gggcggcgag cgtgagcgcg acccaggcgc
     8761 cggtcaccgc cgcggtgcgc gtgccgccgt ccgcctggag gacgtcgcag tcgagccaga
     8821 ccgtgcgctc gccgaggttc gcgagatcgt aggcgccgcg gatcgagcgg ccgatcaggc
     8881 gctggatctc gaccgtgcgg cccttctgcc tgccggcgct cgcctcgcgg cgcatccgct
     8941 cgccggtcga ggcggggagc atcccgtact cggccgtcag ccagccctgg cccttgccct
     9001 cgagccagcg cgggacgccg tcctccacgg tcgcggtgca gaggacgcgc gtgcggccga
     9061 gcgagatgag cgccgagccg tccgcctgct cgaggaagcc gggggcgatc tcgagcggcc
     9121 gcagctcgtc cggccggcgt ccgtcggcgc gcgtcacgcg gcctcgaggg cgagctcgtc
     9181 gagcgcgacg cggcgcgcgc ccgcgacggg aagctggagg aagcgctcgc cgagcgccgc
     9241 gaactccgcg ggggagccgg tggtcaggaa cgtcgtctcc ccgtggcgca ccgggtcgtt
     9301 gccgacgccc ttccggtgga gcgtctccgc cacctcgcgc gcggtctcgt cggccgagaa
     9361 gacgagcgtc gtcccacgtc cgaagacccg ctcgaagacg gggcgcacga gcgggtagtg
     9421 cgtgcagccg aggacgaccg tgtccacctg cgcgtcccgc agcggcgccg cgtactcgcg
     9481 gacggccgcg aggagctcct cgttcggccc gcccgcctcg atgagcggga cgagcttcgg
     9541 gcacgcgacg gagacgacct cgacccccgc gtcgagcgtg tggatgacgt ccgcgtaccg
     9601 gccgctctcg atcgtcgcct cggtggcgag gacgccgacg cggcggttgc gcgtcgcctg
     9661 cacggccgcg tgggcctccg gcgcgaggac ggcgacgacc ggcacgtcga gccgctcctg
     9721 gaggtacggg agcgccgcgg cggcggcggt gttgcaggcg gcgacgacga gcttcacgcc
     9781 gctccgctcg aggtaggcgg cgagctcgag cgcgaagcgg cggatctcct ccgcctcgcg
     9841 cggcccgtac gggcaccgct ccccgtcgcc gaggtagacg aagtcctcgt gcggaagcgt
     9901 gacgaggcac tcgtgcagca cggtgaggcc gccggcgccc gagtcgaaga tcccgatcgg
     9961 ccgggagtcc atgccggcga ttgtacgagc cggccaggcg gggcctccgc ggacacggcc
    10021 ggtgcctggc accgggcgtg tccgcccggg acgtgcccgg tcgctacagg gccgcgcggg
    10081 ccttggcccg caggtacgcg ccgacgcagg cggcgccgag cccgtccggc ggggcctcga
    10141 tcacgtccgc gccggtcgcg cggatccgcg ccgcgacccg ggcgcgcgcg tccagcacct
    10201 cgaccgcgac cgtggcggcg tacgcgtcga gggccgtctc gggcgcgcgg cgcacgagcc
    10261 ggtcgagatc cgtgtccgcg gcgctcgcga ccgcgaccgc gtgccggcgc cgcaggacgt
    10321 ggatcgcctc gacgagcggc cgtgccgccg cctcctcgag gaggtcggtg aagacgatca
    10381 cgagcgcccg cttagacgag ccgaccgcgc ggaaggcgag ctcgtagtcc gagtcgacgc
    10441 gctccggctc gacgtcgaag agcgcgccga tcaccgaccg cccgcccccg cgccgcggcg
    10501 cgacgttccg cctgagctcg gcgtcgaacg cgatcgcgcc gcagcggtcg ccgagctcgt
    10561 ccgcgacggc cgcgaccgcg gcggccgcgt cgaccgcagc gtcgagccgc gtccggtcgc
    10621 cgagcggcgc cgccatgagc cgcccggcgt cgacgaggca gacgacgtcc cggtcctgct
    10681 cgatccggta ctggttcgac atcggccgtc cgagccgcgc ggtcgcgcgc cagttcacct
    10741 gccggacgtc gtcgtcgggc aggtagtccc gcaccgactc gaactccgtc ccgagcccga
    10801 gcgggccgcg ggtcgcgcgc cccgtgtccc ggaagcggcc ctggcgcacg gcgagcgcga
    10861 ggcggaacgc ggccggaagg tcggggtaga cgagcgcctc cgccgcctcg cccgcgcggc
    10921 gcgtccacgc gccgagcccg agcgggccgg tcagccgcac cgccgccggg ggaagcacgt
    10981 gccggccgcg ccgccgcggc aggaggacgc cgtcgagccg cccgtccgcc tcggacggct
    11041 cgagccggag gtccggcggc acgggctgcc gcagccgcgc cgcgcccggc ggcggctcct
    11101 cgagctcgag ccgaagcggc gccgcgaccc cccgcgagag cgtcgccggc acgctccggc
    11161 ggacgaccgg cgggcggcgc acgagaagcg cgtccacgac ggtcgccgcc agcaccgcga
    11221 ccgcgaggag cgcggcgacc gggagcggga cgaggagcgc cgagagcgcc gccgcgccca
    11281 cgagcaccgc cgcgcgcggg gtcgggctca ccgcgggacg ggcacggccg cgagggcggc
    11341 ctgcaccgcg tcgtccggcc ggtagcgctc gagctccgcc tcgggccgga ggacgagccg
    11401 gtggcggagc acggccggcg ccatccggac gacgtcgtcg ggcgtcacga actcccgccc
    11461 gtcgagccgg gcggcggccc gcgcagcggt cacgagatgg acggcggcgc gcgggctcgc
    11521 gccgagcgtc acgctcggca ggtcgcgcgt cgcccgcacg agcgcgacga cgtacgccgc
    11581 cacctcgtcc gagacggtcg tcgcgtcgag cgtcccgcgc gcgtccgcga gcgccccggc
    11641 gtcgaccacg cccgcgacgt cctcgagcgt cgcgggcacg acgccgcggc ggcgcagccg
    11701 gagcaccgcg tgctcgtcct cctcggacgg gtacccgacg tccaccttga agaggaagcg
    11761 gtcgagctgc gcctcgggga gcgggtacgt gccctcgtac tcgatcgggt tctgcgtggc
    11821 gacgacgagg aacgggtcgg ggagcggatg cggctcgccc tcgatcgtca cctgcgcctc
    11881 ctgcatcgcc tcgaggagcg cggcctgcgt cttcggcggc gtgcggttga tctcgtccgc
    11941 gaggaggagc tgcgtgaaga ccggcccggc gcggaagacg agctccccgc ccttcagcgt
    12001 catcgtcccg gtcaggtccg aggggagcat gtccggcgtg aactggacgc gccggtactc
    12061 gaggccgagc gcccgcgcca ccgcgttcgc ggcgagcgtc ttcgccacgc cggggacgcc
    12121 ctccaggagg acgtggccgc ggcagacgag cgcggcgagc atgagctcga ccgcctcgtc
    12181 ctggccgacg agcaccttcc ccacctcggc gcggacccgg tcgcggagct ccctcacgcg
    12241 cggccgctcc ccgagagccg cgcgagcgcc cgcccggccg cggcgaggcc cgcggcgtcg
    12301 cgcaccgggc cgagcgccgc ctcccgctcg tcctccggga gcccgaaccg cgccgccgcg
    12361 gcccgcacgt cgtcgtccga agcgccggga ggcagcccgg cgcggcgggc gagcgcgcgg
    12421 cgcacggccg cctggagggg cgccgccgcc tcgcccggcc tccgcgagcg cgcgaggagc
    12481 gccgcgagcg actcgacgta ctcgagccgg ggcggcggga ggtcgcgctc cgcgtcctcg
    12541 gccggcccga gccgccggcc gcgcgcccac atgaagagga gcgccgcgag gccgagcccc
    12601 gcgagcgccc aggcccagcg gctcggaatc gccccgagcc cgctcccgcg cccgtagccg
    12661 tgcacgctct ccacgaagac gaccgggcgg cgcggctcgc cggccgccgc gagcccgaac
    12721 gccgcgttgt ccgcgcgggc gagcaggcgg ttctggagga tcgaggggtc ggcgagcagc
    12781 gtcatcgtcc cgcgcccgcg ccgtgcgacg accacgaccg cctgccggcc gcctgcgagc
    12841 acggggagcg cctcgcccgc atcctcccac accccgcggc cggcggtctc gaccgtctcg
    12901 acgccgcgca cgtcgtccgt cgggacgacg acctcggtgc gccgcgggcc cgtgggcgcc
    12961 cacgccggcg gctccggcag gagctccgcc acccaccgcc cgggcgcggc gccgccgccg
    13021 aggaggtgcc cgccgtcctc gacgaagcgc gcgagcgccg cacggtcgtc gccgtcgagc
    13081 gggcccgggt cgaggaggac gagcgtgacg gccgggtcga gctccccctc gccgagcggg
    13141 tcgcggagcc gctcgaccgg atgcccgctc cgcgcgagca ggtcggcgta cgcggccacg
    13201 ccctcgggcg ccgtcgcgta cgacgaggac gggggccccg cgggcacccg cgtcagctcg
    13261 gcgagcgcgg ccagcaggag gttgaccgcg acgacggcgc cgacgacgag cgcgacgacc
    13321 gtccgggcgc gggcggggcg tctcatcgcg tgcccacctc ggcgaggacg cgccgccagc
    13381 cggcgcgcga ggcctccacg tcggccggct cgggcgggcg gcgcccgtac gcgacctcgt
    13441 cgaagacgcg cgcgaccccg tcgaagtccg gcgagcggag ctcgcggcgc acctggccgg
    13501 tcgtgagcga gtcgcgcagc acgatcgcgc gcgcccgcgc gaggcgcacg agcccggcgc
    13561 ggaagaggag ccggaccgcg cgctcgaggt cgccgctccg ctccgcctcg gccgcctcct
    13621 cctcgagccg gcggacgtcc acctcccccc gcgcggacgc cgcccggccg cgctcgaccg
    13681 cgtccgcggc gcgccggcgc ccgacgcgcg cggccgcgag cgccgcgagc gcgacgacga
    13741 ccgccccgag gagcacccag aagaccggcg gccccccggg aacccggccc gcgagcgcgt
    13801 cgatccgctc gccgagccac gcgagcggcc ggcggaacgg ccgcggcacg tcgggctcgc
    13861 ggaagcggcg ctcggagagg atcgcgcgcg cgtcgtcctg cgcgcgggcg ggcggggcgg
    13921 ctcccggcga cgcctccgcc ccgtcggcga gcgcctcgag acggcccgcc agctcctcgc
    13981 ccccggcgcc ctcgagcgcg agctcgaggt cggccggcgc gccgtccacc cgctcgacgt
    14041 cccggagctc ctcgagcgcc gccggatccg tctgcgcccg ggcggcgagg tcgcggagct
    14101 cgccgcccgt cacctcctgc gcgcccgcgg gggcggcggt caggagcgcc gcaaccgaga
    14161 cggcgacgaa cgcgagccgg gcgctcactc gtcccgcccg ccgggcttcc agccgggggg
    14221 cggcggccag tacggcggcc gctcgggctc cggcgacgtc tcgccgcccg gcggaggccc
    14281 ctcctcggag gacggccgcc accccggcgg cggaggccag tagggcggct gctcgccgct
    14341 cgacggcgag ggcgcggccc ggggcgcgag cgacacgcgg tccgggtcgg gcgcgacccc
    14401 gatccgctcc gcgaggagcg cgaggtcgaa gccctccttc cgcacccgga ggtcgaagta
    14461 gaggacggtg gcgaacgcgg cgatgagcgg cgtccagagc gcggcggcga gcgtctgccc
    14521 gaccgcgttc gccgtgagca cgagcaccgc gctgtcccca gcgtcggtga acgccggcgc
    14581 gaacgcgagg aactgcacgg cgtacgagag gaagccggcg atcatcaccc cgacgagcat
    14641 gagcccgaac gccggccacc acctgttccg ggtgagccgg aaggagcgcc cgaccgcctt
    14701 gagccccttc ctcccctccg tgagcagcac cgggatcgcg acgacccagg cgaccgcgat
    14761 ccagatcccc ggcaggaggc agaggagcag cccgagaccg accgtgaagg cgtagatcag
    14821 gctgatccag aggagcgagt gcagccggcg cagcgcgtag ccgagagacg tcctccagtt
    14881 cggcgtctcg ccgaggtagg cgtcggcgac cgccttgtag caggcgccgg tggcgagcgt
    14941 ggtcgcgacg aagccgagca gcgcaccgac gaggatcgcg gcgaaccagg ccgcgaactc
    15001 ggcgtcc
//
LOCUS       DASTFC010000106         6352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1254224,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000106 DASTFC010000000
VERSION     DASTFC010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254224"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..1707
                     /locus_tag="VFO88_03125"
     CDS             373..1707
                     /locus_tag="VFO88_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544547.1"
                     /translation="MRTARSSKRPAGFRAPILLVVLVALWAAPSARADASPVLDAPAD
                     GETVTLPTTLALTLPAEGWVRVQISAGETFDAASLACDSGWYHSGFHTSSSWTLPGVI
                     GYSDVTCELEPGAYRWRAAYGGPTGEVPSDDPATWSDVASFSIFGGAPAPAPPPPAPA
                     PYEPEYDEQEEALCAAESEEDPDGGDPGEIEFSQDDVEPTAEELAIGTPPPAEPETFG
                     SLATVGESESAGESDGEEADAGFDATGATECSRREILRWVGVAEFHVALGFQGAFTYQ
                     DGRETKTQVALQVPGRGWTLGGWVMEARNRAAQWNWPRRGPFHRRIAAEYTFRKHVRC
                     SKGGCYAWWAPHQWTGRLRPGAIRIQTTGWNNRYKFRLPHGHTWTKWLGTNKTMDKAA
                     YVAGVTLSAQAGYSTITKMTWRSTPRCEENWIDPINDHASSAQLIFTWSDRC"
     gene            1689..2324
                     /locus_tag="VFO88_03130"
     CDS             1689..2324
                     /locus_tag="VFO88_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544548.1"
                     /translation="MERQVLKLLPGLAVAALLASGCGGEQGPVAGDALEVGAASTYAV
                     EGAGALPPGVEVSLMLTPLRNVSDGDLVIRRIEPIGGIPTDGEGAAAEVVGFELAVRD
                     QALATAVPLGFYAGARPALQVDGACVEQPTVAPEDFVLAPVGERDEDVFVVLRVRTTA
                     EGVFGLDGLRVVYELSGVQYFQELRDGLVLHVVPGAAPDVPEAQTACLAGG"
     gene            complement(2342..3208)
                     /locus_tag="VFO88_03135"
     CDS             complement(2342..3208)
                     /locus_tag="VFO88_03135"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HET9544549.1"
                     /translation="MVAETSAKPSGAASEAASEAKQAARGPWVERLGRAGLVAQGVSY
                     GLVGTLALMLALGLGGEAESREGALRTLAGNTAGLAVLVALAIGFAGYAIWRLAQAIF
                     DRDNEGTDGKGLAKRASRAGKAVIYAALCFTTVQIVLDGRRSSSGGGGEPTEDSATAG
                     VLGWPAGRWIVLGVGVAILGVAGYQVWRAFSEKFMKDMETSRLSPAAETAVEALGITG
                     LLARGVVFGLVGIFLVKAALEYDPDEAIGLDGALATLAGASYGSVLLGLTAAGLVAYG
                     LFCVVQARYRRI"
     gene            complement(3310..3753)
                     /locus_tag="VFO88_03140"
     CDS             complement(3310..3753)
                     /locus_tag="VFO88_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544550.1"
                     /translation="MSTLAKLLLVPAAVLVAVAGVALAGGASSAFEGGSSGTVSTDAT
                     TTDTVGTETGSRVEISGPCDEAEHANDPRCAGVVLPAPAAGAAAGGVDISGPCDELEH
                     ANDPRCTGAGRAADDNSGPGSASSGRDRDDDRGRGRGRGRGGDRD"
     gene            4280..4666
                     /locus_tag="VFO88_03145"
     CDS             4280..4666
                     /locus_tag="VFO88_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544551.1"
                     /translation="MPLLTRLALLILAGAGVLAALGCGGAGGDKTFEGDGYSFSYPGD
                     WDEGTARQEGAASGRSNAAVSPPGSEESFVSSADAHRVCDLTAEDFADRGVLIFRCID
                     EAQAEREVNALRAAWESENGIPPGRG"
     gene            complement(4777..4848)
                     /locus_tag="VFO88_03150"
     tRNA            complement(4777..4848)
                     /locus_tag="VFO88_03150"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4814..4816),aa:Val,seq:cac)
     gene            4896..5160
                     /gene="ffs"
                     /locus_tag="VFO88_03155"
     ncRNA           4896..5160
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFO88_03155"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            5214..>6352
                     /gene="dnaX"
                     /locus_tag="VFO88_03160"
     CDS             5214..>6352
                     /gene="dnaX"
                     /locus_tag="VFO88_03160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9544552.1"
                     /translation="MSGALYRTYRPQTFDEVVGQEAVVRTLRNAIEREQVRQAYLFAG
                     PRGTGKTSLARILAKALNCAYGPTATPCGECNSCVSIAAGTSLDVVEMDAASQRGIDD
                     IREIRDRVVLQPVEGRHKVYILDEAHQLTDAAFNALLKLIEEPPPHLVFVFCTTDLAK
                     MLPTVRSRCQTFMFARPRLPELTRLLRRIADGEKIDAPDAALSLVARSARGSFRDAVS
                     TLDQLAASTDGAVTVQAVLQLLGAVEEEALFRIGDLIVDGDTAGLLLFVEELADQGQD
                     LNRLVQDLLEHLRHLLLVQHMGEVPDSLPVTEETRERIRSQANQLGEPVVLRLIDLLA
                     VAVDDARQGGDPRLPLELALVKVTRPGAELTRESLAYRLEQLEARA"
BASE COUNT          918 a         2316 c         2270 g          848 t
ORIGIN      
        1 cgaggccggg cgcgagccgg ctcagcgcga gcacggtctc gagccctgcg acgccgccgc
       61 cggcgaccac gacacggtgc cggccgcgga gggatggcgt cgtcctcgtc atgagccgca
      121 gtgtcgcggc gacggatgcg cacgccatcg ggaggacgcc gcaggccgtt gcgggaaatc
      181 acggatacca ccgctgctcg ggtgactgcc gcagccgcgc gcccttttcg gtcacggctt
      241 ccgtgccgaa acgcacacga atctgtgcca agagcgtggc ggacgagccc ggaggcgccc
      301 tggcccgcta cggtgctcga gaagcccgct cccgctgacg ccaaggcccg cacgaacgcc
      361 aaggaggttg gcatgagaac cgctcgatcc tcgaagcggc ccgcgggttt ccgagcaccc
      421 atcctgctcg tagtcctcgt cgccctctgg gcggcgccgt ccgcccgggc cgacgccagc
      481 ccggtgctcg acgcacccgc cgacggcgag accgtcacgc tccccaccac cctcgccctg
      541 acgctcccgg cggaaggctg ggtgcgagtg cagatctcgg cgggcgagac gttcgacgcg
      601 gcctcgctcg cctgcgactc cggctggtac cacagcggct tccacacgtc gtcctcctgg
      661 acgctgcccg gcgtcatcgg ctactcggac gtgacgtgcg agctcgagcc gggcgcgtac
      721 cgctggcgcg cggcctatgg cggaccgacc ggcgaggtgc cgtcggacga ccccgccacg
      781 tggagcgacg tcgcgagctt ctcgatcttc ggcggcgctc ccgcgccggc tccgccgccg
      841 cccgctcccg cgccttacga gccggagtac gacgagcagg aggaggcgct ctgcgccgcg
      901 gagagcgagg aggacccgga cgggggcgac ccgggcgaga tcgagttctc gcaggacgac
      961 gtcgagccga cggccgaaga gctcgcgatc ggcacgcccc cgcctgccga gcccgagacc
     1021 ttcggatcgc tcgcgaccgt cggcgagagc gagagcgccg gcgagagcga cggcgaggag
     1081 gcggacgccg gattcgacgc gaccggagcg accgagtgct cgcggcggga gatcctccgc
     1141 tgggtcggcg tggccgagtt ccacgtcgcg ctcggcttcc agggagcctt cacctaccag
     1201 gacggccgcg agacgaagac gcaggtcgcc ctgcaggttc ccggccgcgg ctggacgctc
     1261 ggcggctggg tgatggaggc gaggaatcgc gccgcgcagt ggaactggcc tcgccgcggc
     1321 ccgttccacc ggcgcatcgc ggcggagtac accttccgca agcacgtccg ctgctcgaag
     1381 ggaggctgct acgcgtggtg ggctccgcac cagtggacgg ggcggcttcg gccgggcgcg
     1441 atccgcatcc agaccacggg ctggaacaac cggtacaagt tccgcctccc gcacggccac
     1501 acctggacga agtggctcgg cacgaacaag acgatggaca aggcggcgta cgtcgcgggc
     1561 gtcaccctga gcgcgcaggc gggctactcg acgatcacga agatgacgtg gaggtcgacg
     1621 ccccggtgcg aggagaactg gatcgacccg atcaacgacc acgcgtcgag cgcgcagctc
     1681 atcttcacgt ggagcgacag gtgctgaagc tccttccggg gctggccgtc gccgccctgc
     1741 tcgcctccgg ctgcggaggc gagcagggcc ccgtcgcagg cgacgccctc gaggtcggcg
     1801 cagcctcgac gtacgccgtc gagggagcgg gcgcgctgcc gcccggcgtc gaggtgtcgc
     1861 tgatgctgac gcccctccgc aacgtgtccg atggcgacct ggtcatcagg cgaatcgagc
     1921 cgatcggcgg gatcccgacc gacggggagg gggcggcggc cgaggtcgtc ggcttcgagc
     1981 tcgccgtgcg cgaccaggcc ctcgcgacgg ccgttccgct cgggttctac gccggagcgc
     2041 gtcccgccct ccaggtcgac ggcgcgtgcg tcgagcagcc gaccgtggcg ccggaggact
     2101 tcgtcctcgc ccccgtaggc gagcgcgacg aggacgtgtt cgtggtgctg cgcgtccgga
     2161 cgacggccga gggggtgttc ggcctcgacg gcctgcgggt cgtctacgag ctgagcggcg
     2221 tgcagtactt ccaggagctc cgagacgggc tcgtcctcca cgtcgtcccg ggcgccgcgc
     2281 ccgatgttcc cgaggcccaa accgcctgcc tcgccggtgg gtgacggcgt gcggccgttg
     2341 ctcagatgcg gcggtagcgc gcctgcacga cgcagaagag gccgtaggcg acgaggcccg
     2401 ccgccgtcag cccgaggagg accgacccgt acgaggcccc ggcaagcgtg gccagcgcgc
     2461 cgtcgagccc gatcgcctcg tcggggtcgt actcgagcgc ggccttgacg aggaagattc
     2521 cgacgagccc gaacacgacg ccgcgggcga ggaggccggt gatgccgagc gcctcgacgg
     2581 cggtctcggc ggcggggctg agccggctcg tctccatgtc cttcatgaac ttctccgaga
     2641 acgcgcgcca gacctggtag ccggcgacgc cgaggatggc gaccccgacg ccgagcacga
     2701 tccagcgccc ggccggccac ccgagcacgc ccgccgtcgc gctgtcctcg gtcggctcgc
     2761 cgccgccgcc cgagctcgac cggcggccgt cgaggacgat ctggacggtc gtgaagcaga
     2821 gagccgcgta gatgacggcc ttccccgcgc ggctcgcccg cttcgcgagg cccttcccgt
     2881 cggtgccctc gttgtcgcgg tcgaagatcg cctgcgcgag ccgccagatc gcgtacccgg
     2941 cgaacccgat cgcgagggcg acgaggaccg ccagcccggc cgtgttgccc gcgagcgtcc
     3001 ggagggcgcc ctcgcggctc tccgcctcgc cgccgagccc gagcgcgagc atgagggcga
     3061 gcgtgcccac gagcccgtag gacacaccct gggcaaccag tccggcgcgt ccgagccgct
     3121 cgacccaggg gccgcgcgcg gcctgcttcg cctcgctcgc cgcctcgctc gccgcgccgg
     3181 aaggcttggc cgatgtctcc gctaccacgg acgggagtgt gcccctcacc cacgtccgcc
     3241 gaaacgcagg ccggcgcggc cggggagggg aggacgtccg ccggccgcgc ccgcgtgcgc
     3301 gcgtccgggt cagtcccggt cgccgcccct gccacggcct cgacccctgc cgcggtcgtc
     3361 gtcccggtcg cgacccgagc tcgcggagcc cggccccgag ttgtcgtccg ccgcccggcc
     3421 ggcgccggtg cagcgcgggt cgttcgcgtg ttcgagctcg tcgcacggcc cgctgatgtc
     3481 gacgccgccc gcggcggctc cggcggccgg cgccggcagg acgacacctg cgcagcgagg
     3541 atcgttcgcg tgctcggcct cgtcgcacgg gccgctgatc tcgacgcgag agccggtctc
     3601 cgtgccgacc gtgtccgtgg tggtcgcgtc cgtcgacacg gttccggacg agccgccctc
     3661 gaacgcggac gacgcgcccc cggcgagcgc caccccggcg acggcgacga ggacggcggc
     3721 cggcacgagc agcagcttgg cgagcgtgga catgatccct cctccgttcg gttggacctg
     3781 tccctcgaag ctacgaggcg ccgccccaag cccgctcccc gcgagggcga agattcggcc
     3841 agacgacggt taagacggga atgggcgcac ctgggatcga acacccctgc aaatcgaggg
     3901 ggccgtcgcc tcgaggtacg cacgggggta cgcacgagac gagcgattcc gggccgcggc
     3961 gtgcgcgccg ctctacgcca actgcagtac gcgctcttcg actagaggga cgctgatacg
     4021 gatatcgcgt ccttccgacc gagcgcggaa gtcatagtcg gagacgagcg gtgtcccctc
     4081 gagctcgaaa gtcacaaccc tcaccctccg caggcgttcg cacacgtcct ccgggcggag
     4141 aagcacggtt gcgggcatct ctccgcgaaa ccttatattc cctacaggat agactgactt
     4201 taccccgaga ggacgggttt caaggccggc cggtaacctg ccggcgacct tgcgcccggg
     4261 ccgaacccga tctaaggttg tgcctctgct cacaaggctc gcacttctca ttctcgccgg
     4321 cgctggcgta ctcgccgccc tcggttgcgg aggtgcgggt ggtgacaaga ccttcgaggg
     4381 cgacggctac tccttcagct accccgggga ttgggacgag ggtacggctc gacaggaggg
     4441 ggcggcgagc ggtcgctcca acgccgccgt tagtcctccc ggtagcgagg agagctttgt
     4501 gagcagcgcc gacgcccaca gggtctgcga tctgacggcg gaggacttcg ccgatcgcgg
     4561 cgtactgatc ttccgctgta tcgatgaggc gcaggccgag cgcgaggtga acgctctccg
     4621 cgctgcctgg gagtcggaga acggcatccc gccggggcgg gggtagccgg attccggggt
     4681 agcaccgggg tacgcacgcg acgtgcatcg acggcacgta cgttcgcgtc cgtaccgctt
     4741 gaaatcaggg aacccgtgtg tccatgcggc tgggagtggg cgcacctggg atcgaaccag
     4801 ggacctcccg cgtgtgaagc gggcgctctc ccgctgagct atgcgccccg aggaggcgga
     4861 cgatagcaac ggcggtgcta ccatccacgg gccggtcgcg ctaggcgggg agctagcggt
     4921 tccctgaacc cgcaatccgc tccagcgggg ctgaatcccc tcccgagggg gtggctgcaa
     4981 ggtagggctt gcgcacgtga gcgacgacgg ccaggtcccg cgcactgaga gcctgcgaac
     5041 cgcgtcagat ccggaaggaa gcagcgctaa gcggagcgcc tcaggtgccg cgggtcagcc
     5101 tgacccgagc gagcgaacgc gggcaccgcc gcgcaagcgc ccacgacggg gggtgcgcga
     5161 ccggcatttt ccacccgttt cggccgcccg ctcgcctgct ctaggatggc ctcgtgtcgg
     5221 gcgcgctcta ccggacgtac cggccgcaga cgttcgacga ggtggtcggg caggaggcgg
     5281 tcgtccgcac cctgcggaac gcgatcgagc gggagcaggt tcgccaggcc tacctcttcg
     5341 cgggcccgcg cggcaccggc aagacgtcgc tcgcccgcat cctggccaag gcgctgaact
     5401 gcgcgtacgg cccgacggcc acgccgtgcg gcgagtgcaa ctcctgcgtc tcgatcgcgg
     5461 ccgggacgtc gctcgacgtc gtggagatgg acgccgcgag ccagcgcggc atcgacgaca
     5521 tccgcgagat tcgcgaccgc gtcgtcctcc agccggtcga gggccggcac aaggtctaca
     5581 tcctcgacga ggcgcaccag ctgaccgacg ccgcgttcaa cgcgctgctg aagctgatcg
     5641 aagagccgcc gccgcacctc gtgttcgtct tctgcacgac cgacctcgcg aagatgctcc
     5701 ccacggtccg gtcgcgctgc cagacgttca tgttcgcgcg cccgcggctg ccggagctga
     5761 cccgcctcct gcgccggatc gccgacggcg agaagatcga cgcacccgac gccgcgctct
     5821 cgctcgtcgc gcggagcgcc cgcgggagct tccgcgacgc cgtctcgacg ctcgaccagc
     5881 tcgcggcgtc gacggacggc gcggtcaccg tgcaggcggt gctccagctc ctcggcgcgg
     5941 tcgaggaaga ggcgctcttc cggatcggcg acctgatcgt cgacggcgac accgccgggc
     6001 tgctcctctt cgtcgaggag ctcgccgacc aggggcagga cctgaaccgg ctcgtccagg
     6061 atctcctcga gcatctgcgc catctcctgc tcgtgcagca catgggcgag gtgcccgact
     6121 cccttccggt cacggaggag acgcgcgagc ggatccgctc ccaggcgaac cagctcggcg
     6181 agccggtcgt cctgcgcctc atcgacctcc tcgcggtcgc ggtcgacgac gcgcggcagg
     6241 gcggcgaccc gcgcctcccg ctcgagctcg cgctcgtcaa ggtgacgcgg cccggcgccg
     6301 agctcacgcg cgagtccctc gcctaccggc tcgagcagct cgaggcgagg gc
//
LOCUS       DASTFC010000107         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_380777,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000107 DASTFC010000000
VERSION     DASTFC010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380777"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFO88_03165"
     CDS             complement(<1..288)
                     /locus_tag="VFO88_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544553.1"
                     /translation="MGLGNILFGKKKLSGAKLDKLFALSTAHVQMQAELGLRSAGAAA
                     VVFKPLSAGDFMRAEQELDELLGVAAASSGSEVRRRQDSFGFQWIVVRDNDF"
     gene            complement(464..1372)
                     /gene="htpX"
                     /locus_tag="VFO88_03170"
     CDS             complement(464..1372)
                     /gene="htpX"
                     /locus_tag="VFO88_03170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET9544554.1"
                     /translation="MRRKAFGRDAGLSARMFGTMFLLGLLYVVFFVVLISLFELQYWF
                     VILLLGGLAFLQYYTSDKIALAASRAKVVTPEQAPELHAMVDRLCAMADLPKPKVAII
                     PTDVPNAFATGRNPKNSAVAVTEGLWRRLEPREVEGVLAHELAHISNRDVAIMTVASF
                     FAMVAAFITRMAVWSGMMGGGRGRDNGPPVFLIVLAVSIVTYVISYVLILALSRYREF
                     AADRGAALITGAPEQLMSALQKIASQMTRIPQRDLRELEGMNAFYIVPTNVKQSIGQL
                     FMTHPPLEKRLERLGSIAAEMGRPVA"
     gene            1448..1861
                     /locus_tag="VFO88_03175"
     CDS             1448..1861
                     /locus_tag="VFO88_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9544555.1"
                     /translation="MRRLPLLALTLLLTLVLAACGGSEERDTSPEDVQGETPTTETQP
                     EDDGNGEDDGGGGEGDAEAGAALFEEQGCGSCHVLADAGSTGTTGPSLDEAQPSYDAA
                     FQQIQNGGGGMPAYDGLGEDAIADLAAYVSERAGG"
     gene            1914..2699
                     /locus_tag="VFO88_03180"
                     /pseudo
     CDS             1914..2699
                     /locus_tag="VFO88_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            2696..>3420
                     /locus_tag="VFO88_03185"
     CDS             2696..>3420
                     /locus_tag="VFO88_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544556.1"
                     /translation="MIDVKAARAEPDRYREALVRKGAGESFDALMAADAEWLTLVPRA
                     DELRARMKLKGKPTPEQLEGLKAAKAELQDVERALAEAQERRDALLAEVPNPPDASAP
                     DGTADEDAEEIRRVGEPAELGFEPRDHLELARIDTERGARLSGSRFAYRIGAVARLEL
                     ALYRFALDRIGERGFLPVLPPVLVREEAMYGTGFLPTEEVNIYRVERDDLYLTGTSEV
                     ALAGLHMGEILEADELPLRYAGYS"
BASE COUNT          507 a         1215 c         1245 g          453 t
ORIGIN      
        1 gaagtcgttg tcgcggacga cgatccactg gaacccgaac gagtcctggc ggcgccgcac
       61 ctccgagccg gagctcgcgg ccgcgacgcc gagcagctcg tcgagctcct gctcggcccg
      121 catgaagtcg cccgcggaga ggggcttgaa gacgaccgca gcggcgccgg cgctccggag
      181 gccgagctcc gcctgcatct ggacgtgcgc ggtcgagagg gcgaagagct tgtcgagctt
      241 cgcgccggag agcttcttct tgccgaagag gatgttgccg agacccacgc gggtgagtct
      301 aggcgccgcg cgcgaaacgc ccgtgggtca gctggcggcc cgaaccgcgt gccccgccct
      361 caggggtggg gtcgaagggg tccgacccgc ctcccgcctc gagcgtagcg ccgaaaagcg
      421 cacggaaccg tgccgatccg tccacaggcg cggcggcgag gcgctacgcg acggggcggc
      481 ccatctcggc cgcgatcgag cccagccgct cgaggcgctt ctcgagcggc gggtgggtca
      541 tgaagagctg tccgatcgac tgcttcacgt tcgtggggac gatgtagaac gcgttcatcc
      601 cctcgagctc gcggagatcg cgctgcggga tccgcgtcat ctggctcgcg atcttctgga
      661 gggcgctcat gagctgctcc ggcgcccccg tgatgagcgc cgcgccgcga tcggcggcga
      721 actcgcggta gcgcgagagc gcgaggatga ggacgtacga gatcacgtac gtcacgatcg
      781 agacggcgag gacgatgagg aagaccggcg gtccgttgtc gcgcccgcgg ccgccgccca
      841 tcatcccgct ccacacggcc atccgcgtga tgaacgcggc gaccatggcg aagaagctcg
      901 ccacggtcat gatcgcgacg tcgcggttcg agatgtgcgc gagctcgtgc gcgaggacgc
      961 cctcgacctc gcgcggctcg agccggcgcc agaggccttc cgtcacggcc acggccgagt
     1021 tcttcgggtt ccggccggtc gcgaacgcgt tcgggacgtc ggtcgggatg atcgcgacct
     1081 tcggcttcgg cagatcggcc atcgcgcaca ggcgatcgac catcgcgtgc agctcgggcg
     1141 cctgctccgg ggtgacgacc ttcgcgcgcg acgcggcgag cgcgatcttg tccgacgtgt
     1201 agtactggag gaaggcgagc ccaccgagca gcaggatcac gaaccagtac tgcagctcga
     1261 acaggctgat gaggacgacg aagaagacga cgtagagcag gccgagcagg aacatcgtcc
     1321 cgaacatgcg ggccgagagc cctgcgtcgc gtccgaatgc cttccgcctc atcgaggccg
     1381 attctaccgg cggggggtcg cgccaccgga ggttgccttc ccgcgtcgat cggtaatatc
     1441 ccgcgcgatg cgccgccttc cgcttctcgc gctgactctg ctcctcacgc tcgtgctcgc
     1501 ggcctgcggt ggctccgagg agcgcgacac gagcccggag gacgtccagg gcgagacgcc
     1561 gaccacggag acccagccgg aggacgacgg aaacggcgag gacgacggcg gcggaggcga
     1621 gggcgacgcc gaggcgggcg cggcgctctt cgaggagcag ggctgcggaa gctgccatgt
     1681 cctcgcggac gcgggctcga ccggcacgac gggccccagc ctcgacgagg cgcagccgtc
     1741 gtacgacgcg gcgttccagc agatccagaa cggcggcggc gggatgccgg cctacgacgg
     1801 gctgggcgag gacgcgatcg ccgacctcgc cgcgtacgtc tccgagcgcg ccggcggcta
     1861 gcgccacgct ccccgagggc ttcccgcgcg ccgtccaggc gttcgcgtgc gacttcgacc
     1921 gcacgctcgt cggcgaggac ctcgtcctgc ggccgcgcac gctcgcggcc ctcgccgccg
     1981 cccgcgacgc cgggctcccg gtgatcgtcg tgacgggccg gatgttccgc tccgtccgcc
     2041 cgtacctcgc cgaggcgcga atcgagacgc ccgtcgtctg ttaccagggt gccgtcgtgg
     2101 ccgagccgga gaccggccgg ttcctgcgcc acgagccgat cccgctcgag ctcgcgcgcg
     2161 aggcgatcgc ggccgtggag gaggagggct tcgggctcaa ctgctacgtc ggcgacgagc
     2221 tctacgtcgc gagcgagacg ccggaggcgc gccgctacgc ggagttccag cacatcccga
     2281 tccacgtggt cgggccgctc ctcgactggc tagacgagcc gcccacgaag ctcgtcacga
     2341 tcggcccgcc ggccgtgctg gacgggctcg aggagcgcct caaggcccgc ttcgacggcc
     2401 ggatgttcat ctcgaagtcg ctgccctact tcctcgagct cgcgagcgcc gacgtgacga
     2461 agggctcggg aatggacttc gtcgccgagc acctcgggct cgagccggag cgcgtggtcg
     2521 ccttcgggga cggcgagaac gacgtcgagc tgctcgagtg ggccggatac gccgtggccg
     2581 tcgcgaacgc gcacgagcgg gtgctcgcgg ccgccgacca cgtctgcccg cccgtcgacg
     2641 aggagggcgt ggcgcaggtg atcgaggcct atctcgcgct cgtagactct ccccgatgat
     2701 cgacgtcaag gccgcgcgcg ccgagccgga ccgctaccgc gaggcgctcg tccgcaaggg
     2761 cgcgggcgag tcgttcgacg cgctcatggc cgcggacgcg gagtggctca cgctcgtccc
     2821 gcgcgccgac gagctccggg cgcggatgaa gctcaagggg aagccgacgc ccgagcagct
     2881 cgagggcctc aaggccgcca aggccgagct ccaggacgtc gagcgcgcgc tcgccgaggc
     2941 gcaggagcgc cgcgacgcgc tgctcgcgga ggtcccgaac ccgccggacg cgagcgcccc
     3001 ggacggaacg gccgacgagg acgccgagga gattcgccgt gtcggcgagc cggccgagct
     3061 cggcttcgag ccgcgcgacc acctggagct cgcccggatc gacaccgagc ggggcgcgcg
     3121 gctctccggc tcgcgcttcg cgtaccggat cggcgcggtg gcgcggctcg agctcgcgct
     3181 ctaccgcttc gcgctcgacc ggatcggcga gcgcggcttc cttcccgtcc tcccgccggt
     3241 gctcgtgcgc gaggaggcca tgtacggcac cggcttcctc ccgaccgagg aggtcaacat
     3301 ctaccgcgtc gagcgcgacg acctctacct cacgggcacg tccgaggtgg ccctggccgg
     3361 gctgcacatg ggcgagatcc tcgaggcgga cgagctgccg ctccgctacg ccggctactc
//
LOCUS       DASTFC010000108         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_792958,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000108 DASTFC010000000
VERSION     DASTFC010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792958"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFO88_03190"
     CDS             complement(<1..182)
                     /locus_tag="VFO88_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544557.1"
                     /translation="MAATEMKPGSYIGARVARKEDRKLLTGQSRFIDDLTLPGMVWAS
                     VVRSPFAHARITSVDVS"
     gene            complement(236..691)
                     /locus_tag="VFO88_03195"
     CDS             complement(236..691)
                     /locus_tag="VFO88_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9544558.1"
                     /translation="MRRISVTVNGERHEAEVEPRQLLVYFLREELGLTGTVVGCDTSS
                     CGSCTVLLGGESVKSCTLLAVQADGAEVTTIEGLARDGELHPVQQAFHEQHGLQCGFC
                     TPGMVLATVSLLAESPSPSDGEIRLALEGNLCRCTGYQNIVKAVKAAAV"
     gene            complement(688..1251)
                     /locus_tag="VFO88_03200"
     CDS             complement(688..1251)
                     /locus_tag="VFO88_03200"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9544559.1"
                     /translation="MKLENSFHVDAPAERAWDLLMDVPRVVPCMPGATLDETVDDSHW
                     KATMQVKLGPIGLTFATDVERTVVDEAGRSVTLVANARETRNRGRATATVESSLAAAN
                     GGTRVDIVTDLALSGSVAQYGRGMIEDISSRMVTSFAECLQAQLGESEEDAEAAVAAQ
                     SRPISGLSLFFGSLWRRLGRLVGRRKQ"
     gene            complement(1248..2246)
                     /locus_tag="VFO88_03205"
     CDS             complement(1248..2246)
                     /locus_tag="VFO88_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET9544560.1"
                     /translation="MRDVLDRLERWAADGTPVAVATVVATERSAPRDPGAVLAVSEGG
                     DVAGSVTGGCVEPAVYGEAREVLAGGEPRLKTYGIADEEAFEVGLPCGGTVHIFVDRL
                     DPELVGPIAEAVREERPIALEMRITGEHAGEKRLVGPDDRGPAAQLLARGETGIVDDP
                     AGQLFVSSFAPRPNMYVFGAVDHAAALATIGRFLGFRVTVCDARAKFVTPDRFPDVDE
                     LVVEWPDRFLAGAPVDERTAICVLTHDHKFDVPVLKVALQTSAGYIGAMGSRRTNEDR
                     SERLRAEGVGESELERIHAPIGLKIGSRSPEEVAVAIAAQIVQVLRSPRPQEALTR"
     gene            complement(2243..3088)
                     /locus_tag="VFO88_03210"
     CDS             complement(2243..3088)
                     /locus_tag="VFO88_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9544561.1"
                     /translation="MNRADVIVEAGRLAAAGERYALATVVNVERPASARRGDRAVVTP
                     DGRVTGWIGGACSEPTVVREALRALADGEARLARMEGGCASEGVVEVLVEPVLPAPLL
                     AVCGASPAARTLAELARVVGWRVRDDGVGGADAVVVATMGHGDEELLSAALESGAGYV
                     GLVASAKRAATVLAGLRDAGLDEEAVLRVRAPAGLDLGPSSQEEIAVAILAELVAWRH
                     TRPGESPVPAPAEAVDPVCGMTVSVEGAAATIEHEGGVVVFCSAHCRKAFEAEPERYG
                     GLRAR"
     gene            complement(3085..>3643)
                     /locus_tag="VFO88_03215"
     CDS             complement(3085..>3643)
                     /locus_tag="VFO88_03215"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9544562.1"
                     /translation="IQDIAFAAYTNIPEGMEGGLEGVHYYDPPNMTYPFGAYAVVVEI
                     DRGTGQWNVRKVVAVDDCGVRINPMIVEGQIMGGLTEGFAIAAMELIDFDEDGNCIGA
                     NLMDYLIPTSWETPSFDLGMTETPSPHHPLGAKGIGESATVGAPAAFVNAVVDALAHA
                     GVRNLEMPVTPDKVWAALQEVDLAQ"
BASE COUNT          484 a         1384 c         1286 g          489 t
ORIGIN      
        1 gagacgtcga cgctcgtgat ccgcgcgtgc gcgaacgggc tgcggacgac cgaggcccac
       61 accatcccgg ggagggtcag gtcgtcgatg aagcgcgact ggccggtgag gagcttccgg
      121 tcctccttgc gggccacgcg cgccccgatg tacgagcccg gcttcatctc ggtggctgcc
      181 atcgatcctc ccttctagag ctcgtttcgg aagacttcat ttcgagacga gctctctaga
      241 cggcggccgc tttgacggcc ttgacgatgt tctgatagcc ggtgcaccgg cagaggttgc
      301 cctcgagcgc gagccggatc tccccgtccg acgggctcgg gctctcggcg agcaggctca
      361 cggtcgcgag caccatgccg ggcgtgcaga agccgcactg gaggccgtgc tgctcgtgga
      421 acgcctgctg caccgggtgg agctcgccgt ctcgcgcgag cccctcgatc gtcgtcacct
      481 ccgccccgtc ggcctggacg gcgaggagcg tgcacgactt caccgactcg ccgccgagca
      541 gcaccgtgca cgagccgcac gacgacgtgt cgcagccgac caccgtcccc gtgaggccga
      601 gctcctcgcg caggaagtag acgaggagct gccgcggctc cacctccgcc tcgtgccgct
      661 cgccgttcac cgtgaccgag atgcgtctca ctgcttcctc cttccgacga ggcgaccgag
      721 gcggcgccac agggatccga agaagagcga gagcccggag atcggccgcg actgggcggc
      781 gacggccgcc tccgcgtcct cctcggactc cccgagctgc gcctgcaggc actcggcgaa
      841 gctcgtgacc atgcgggacg agatgtcctc gatcatcccg cggccgtact gggcgacaga
      901 cccggagagg gccaggtcgg tgacgatgtc cacgcgcgtg ccgccgttcg cggcggcgag
      961 cgacgactcg accgtggccg tcgcgcgccc gcggttgcgc gtctcgcggg cattcgcgac
     1021 gagcgtgacg ctgcggccgg cctcgtccac cacggtgcgc tcgacgtcgg tggcgaacgt
     1081 gagcccgatc ggcccgagct tcacctgcat cgtcgccttc cagtgcgagt cgtcgaccgt
     1141 ctcgtcgagc gtcgcgcccg gcatgcacgg gacgacgcgc ggaacgtcca tgaggagatc
     1201 ccacgcccgc tcggccggcg cgtcgacgtg gaacgagttc tcgagcttca tcgcgtgagc
     1261 gcctcctgcg gcctgggtga gcggagcacc tgcacgatct gcgcggcgat cgcgaccgcg
     1321 acctcctcgg gcgagcgcga gccgatcttg agcccgatcg gcgcgtggat gcgctcgagc
     1381 tcgctctcgc ccacgccctc ggcgcgcagc cgctcggacc ggtcctcgtt cgtccgccgg
     1441 ctgcccatcg cgccgatgta gccggccgac gtctggagcg cgaccttgag gaccggcacg
     1501 tcgaacttgt ggtcgtgcgt gaggacgcag atcgccgtgc gctcgtcgac cggcgctccg
     1561 gcgaggaagc ggtcgggcca ctcgacgacg agctcgtcga cgtccgggaa ccgatcgggc
     1621 gtgacgaact tcgcgcgcgc gtcgcagacg gtgacgcgga agccgaggaa gcggccgatc
     1681 gtggcgagcg cggcggcgtg gtcgaccgcg ccgaagacgt acatgttcgg ccgcggggcg
     1741 aacgacgaga cgaagagctg cccggcgggg tcgtcgacga tcccggtctc cccgcgcgcg
     1801 aggagctgcg cggcggggcc gcggtcgtcc gggccgacga gccgcttctc ccccgcgtgc
     1861 tcgcccgtga tccgcatctc gagcgcgatc gggcgttcct cccgcaccgc ctcggcgatc
     1921 gggccgacga gctcggggtc gagccggtcg acgaagatgt ggacggtgcc gccgcacggg
     1981 agaccgacct cgaacgcctc ctcgtccgcg atcccgtacg tcttcaggcg cggctcgccg
     2041 ccggcgagca cctcgcgcgc ctcgccgtag acggccggct cgacgcaccc gcccgtgacc
     2101 gagccggcga cgtcgccgcc ctccgagacc gcgagcaccg cgccggggtc gcgcggggcg
     2161 gagcgctcgg tcgcgacgac cgtggcgacg gcgacgggag tcccgtcggc ggcccagcgc
     2221 tcgagcctgt cgaggacgtc cctcaccgcg cgcgcaggcc gccgtaccgc tcgggctcgg
     2281 cttcgaacgc cttccggcag tgcgccgagc agaagacgac gacgcccccc tcgtgctcga
     2341 tcgtggccgc ggcgccctcg acggacacgg tcatcccgca gaccgggtcg accgcctccg
     2401 ccggcgcggg caccgggctc tcgccggggc gcgtgtgccg ccaggcgacg agctcggcga
     2461 ggatcgcgac cgcgatctcc tcctgcgagg acgggccgag gtcgagcccg gccggcgcgc
     2521 ggacgcggag gacggcctcc tcgtcgagcc ccgcgtcgcg gagtcccgcc aggaccgtcg
     2581 cggcccgctt cgcgctcgcc acgaggccaa cgtagccggc gccggactcg agtgccgcgg
     2641 agagaagctc ctcgtctccg tgccccatcg tcgccacgac gaccgcgtcc gcccccccga
     2701 cgccgtcgtc gcggacgcgc cagccgacga cgcgcgcgag ctcggcgagc gtccgcgccg
     2761 ccgggctcgc gccgcagacc gcgaggagcg gcgccgggag cacgggctcc acgagcacct
     2821 cgacgacgcc ctccgacgcg cagccgccct ccatccgggc gagccgcgcc tcgccgtcgg
     2881 cgagcgcgcg gagcgcctcg cggacgaccg tgggctcgga gcacgccccg ccgatccagc
     2941 cggtcacgcg cccgtccggc gtcacgacgg cccggtcgcc gcgccgcgcc gacgccggcc
     3001 gctcgacgtt cacgaccgtc gccagcgcgt agcgctcgcc cgcggcggcg agccgccccg
     3061 cctcgacgat gacgtccgcg cggttcactg ggcgagatcg acctcctgca gggccgccca
     3121 gaccttgtcg ggcgtcaccg gcatctcgag gttccggacg cccgcgtgcg cgagcgcgtc
     3181 cacgaccgcg ttcacgaacg ccgcgggcgc gccgaccgtc gccgactcgc cgatcccctt
     3241 cgcgccgagc ggatggtgcg gcgagggcgt ctcggtcatg ccgaggtcga acgacggcgt
     3301 ctcccacgac gtcgggatga ggtagtccat gaggttcgcg ccgatgcagt tgccgtcctc
     3361 gtcgaagtcg atgagctcca tcgcggcgat cgcgaagccc tcggtgaggc cgcccatgat
     3421 ctggccctcg acgatcatcg ggttgatccg gacgccgcag tcgtccacgg ccacgacctt
     3481 ccgcacgttc cactgcccgg tgccgcggtc gatctcgacc acgaccgcgt acgccccgaa
     3541 cggatacgtc atgttcggcg ggtcgtagta gtggacgccc tcgaggccgc cctccatccc
     3601 ctcggggatg ttcgtgtagg cggcgaacgc gatgtcctgg atc
//
LOCUS       DASTFC010000109         8515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_693971,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000109 DASTFC010000000
VERSION     DASTFC010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693971"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..700)
                     /locus_tag="VFO88_03220"
     CDS             complement(431..700)
                     /locus_tag="VFO88_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544563.1"
                     /translation="MSTRSCPDWPDLVELAPDLHFKHYTVGEAQVPAEVLAQVPDLSL
                     DTVAVCADLEHHVFNPAHTDERLAAALKETYWYDVHEWAKRTPGG"
     gene            complement(697..1566)
                     /gene="hemH"
                     /locus_tag="VFO88_03225"
     CDS             complement(697..1566)
                     /gene="hemH"
                     /locus_tag="VFO88_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET9544564.1"
                     /translation="MTRTAVVAMAYGSPDRPEDVPAYFADVRGGRPVSQERVDELVER
                     YRRIGFSPLNEVTERQRAALEGELGLPVHVGMKHWKPWIADAVEQALAGGAEELVGLV
                     LAPHYSRISIGGYRDRLERALGGRAALRFIESWHAHEPFVEVIAGRVRGTDGHVVFSA
                     HSLPERIIAEGDPYRDELLTTARLAAERAGIPDERWTFAFQSESPTGEPWLGPDILDH
                     LDALAAAGVGKVLVAPIGFVADHLEILWDIDVEARERAAALGIELDRIESMNDDPAFI
                     SALADLVRKELTA"
     gene            complement(1563..2606)
                     /gene="hemE"
                     /locus_tag="VFO88_03230"
     CDS             complement(1563..2606)
                     /gene="hemE"
                     /locus_tag="VFO88_03230"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET9544565.1"
                     /translation="MTAPLLVRAARREPVERTPVWFMRQAGRSLPEYREIRKGLTLFE
                     ICERPDVCADVTLQPVRRHGVDAAVMFADIMLPPIGMGIEVELVEGVGPVVDSPVASA
                     ADVEALRVPHPEEAFAPLLEAVRIVRGELPPDGAVIGFCGGPFTVAGYLVEGRPSRDF
                     VKTKRCMYGAPEIWHGLMDKLADTFAAYVRAKVAAGADAIQLFDSWVGALSAEDYAEF
                     VAPYSARVLAAVAETGVPSIHFGTGATHLLGELAATGGDVIGLDWRVRIDRGWEAVGA
                     DRGVQGNLDPVLLLGPWNRAAAGAQRIIELVGGRPGHIFNLGHGVLPATDPDVLGRLV
                     ELVHERTAAEVAA"
     gene            complement(2646..3191)
                     /locus_tag="VFO88_03235"
     CDS             complement(2646..3191)
                     /locus_tag="VFO88_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HET9544566.1"
                     /translation="MDHPHLNVQDPWRATALVASGIAALELLLILVLAAVLFIGLSGD
                     DKAPAAAAEKASPTAAAETPAKKGEATKAQPAAKPAKAEPALERGETSVIVLNGNGTT
                     GLAGEKADLVRTKGYLIAGTANAPRTDFARSVVMFRPGYEAEARRLAADFGVPRVAPL
                     DGITNRDLQGAHVALVVGDDA"
     gene            complement(3216..4136)
                     /locus_tag="VFO88_03240"
     CDS             complement(3216..4136)
                     /locus_tag="VFO88_03240"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9544567.1"
                     /translation="MRAVVTGGAGFIGSHLVDALLARGDEVWVLDNLSTGRRERVADG
                     ARLVEGDIRDADAVGAAFAQAEPDACFHLAAQADVRRSVEDPAYDADVNVLGTIRILE
                     AARRHDAQIVFSSTGGAIYGECDAPAPETAARRPISPYGTSKLAGEEYLAMERRLYGA
                     SHVALRYGNVYGPRQDPHGEAGVVAIFLGRLAAGEGFTIFGDGTQTRDYVYVEDVVRA
                     TLAAVGNPGGVFNVGTGRETSVLELADACRAASGVDREPAFADARLGELQRSVLSTAL
                     AERELGFRAEVEPADGIARTWDSIRGEGTP"
     gene            4159..4755
                     /gene="tmk"
                     /locus_tag="VFO88_03245"
     CDS             4159..4755
                     /gene="tmk"
                     /locus_tag="VFO88_03245"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9544568.1"
                     /translation="MFVTFEGVDGSGKSTQARLLVEALRAEGREVVATREPGGTELGE
                     RVRDLLLHGNGLSPWAEATLFAAARAQLVEEVIRPALARGADVVCDRYLDSSLAYQGI
                     GRELGVDRVLELNLVATGGLLPDRTFLLDLPVDEAARRRGGDSDRLEAEGREFAERVG
                     IAYRELAAAFPRRIVRVDATRPPEEQAEIVRAELRDGS"
     gene            4730..5824
                     /locus_tag="VFO88_03250"
     CDS             4730..5824
                     /locus_tag="VFO88_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HET9544569.1"
                     /translation="MQSFETVPEQDEAKRLLAAALAEGPAHAYLFHGPPGVGKRRAAL
                     AFAGELIGDPARVARRSHPDLYVLEPLGDQIRIDPVRDMRRDLHMRPFEAERRVYLVH
                     GAHLMNEDAADALLKDLEEPPPYAVIVLVADELGPLPDTIRSRCQLVPFRRLSRGAVK
                     SWLAARGLEGETLEIAARVAAGRLDRAERLLDAAAGERRRAVLDLARAVYRDPAFEPG
                     AAAAAVGKLAAARAEEARAKAEREAAAAAEAGIEEPKRELDQRLRRAERGAEREEVLN
                     ALDTLAGWYRDLVVVGSGADRAALNSDLLAELAEDAQPDVAVRAARAAEAVRATWRSF
                     EFQVSTGLALEALFVRLRQELASPLPAEAA"
     gene            5821..6645
                     /gene="ricT"
                     /locus_tag="VFO88_03255"
     CDS             5821..6645
                     /gene="ricT"
                     /locus_tag="VFO88_03255"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HET9544570.1"
                     /translation="MSVAVGVVFSPGGRVYSFDPDGLELAWNERVVCQTGNGQELGRV
                     VKGNHELESQPPTPLKKVIRRATEVDRERAAENAEEAKAAMRVFRELLRRFGLDVKPV
                     AVEIGLDGSRAVVSFQAEGRPNTKALQAELARRLGRRIELRSVGPRECARLCGDHGLC
                     GGMQCSRRYPTHEQPITLRMAKDQDLPVASGRITGLCGRLRCCLAFEHPLYKSFRDRA
                     PRVGRRVETPAGVGLVTGYAVLEDTCTVVLDGEETPTPIPVDDCRELAGERRGTKR"
     gene            6676..7842
                     /locus_tag="VFO88_03260"
     CDS             6676..7842
                     /locus_tag="VFO88_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HET9544571.1"
                     /translation="MAVEGTHFEPSPRPDWGGPEPLTAEDCRAAFDAVEPYTIGLEEE
                     LMVVSPETLELSDTAAELVALAADERIRPELRASQLELVTPVCRTAVEACAHLGELRA
                     RVAEVAAGTALLAAAGTHPFSAGTGAVSEGERNELLVEEYEWAALETFACGLHVHVAL
                     GDADRALAVFNALRSYLPEIAALAANSPFARGRDTGLASIRPKLNQAFPREGIPPAFA
                     SWDEYADLVRWGRHAFPDPTFLWWELRPHPAFGTLEVRAADAQTRVEDAAAVAAVVQS
                     LVVSLGRRHDDGERLPAVSSFRISENFWSALRHGLSGWMLDLETGEQEPTLERVARLL
                     AELEPTAGELGLDAELVQARTLLGGNGADRQRYVAAREGLDGLVRWLVAETVLR"
     gene            complement(7839..8207)
                     /locus_tag="VFO88_03265"
     CDS             complement(7839..8207)
                     /locus_tag="VFO88_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544572.1"
                     /translation="MEGSSETLGPARAGAALQAAYYVVTGIWPLVDRGSFERVSGPKA
                     DFWLARTVGALTLAIGLSLGVAAARNRRTPEIETLSLAAPAAFGAIDLVYVARRRISP
                     VYLLDAAAQTVLSALWLRRR"
     gene            8283..8355
                     /locus_tag="VFO88_03270"
     tRNA            8283..8355
                     /locus_tag="VFO88_03270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8316..8318,aa:Thr,seq:cgt)
BASE COUNT         1093 a         3146 c         3176 g         1100 t
ORIGIN      
        1 tcggagccga gcccgctcac ctcgctgaac gcggcgacgg cgatcccgtc gatctcgacg
       61 acgaagttga acgctgtgaa cggatcagac cgagagcccg ccggcggcat cgtctctccc
      121 ttcctagggt gccgcccaag gttactcgcg cgcacgtagc gggtcgcccg ccgtaagggg
      181 gtcagacccc gcactatttc ggctacgaat ccgtgccgac ttcgtaacgt ccctgctcgg
      241 agcgggcttc tcgagacgcg gaaaccgtca cggaagtgtg acgcgcccgc cgttacgggg
      301 tcagaccccg caacggaagc gtcgcgacgc cgggctccgc ccggcgccgc ccggagacgt
      361 tggagttctc acgcggaagt cgtcatggcg cgggcgcgcg agcgcccgcg ccggacttca
      421 cgcgtgctga ctagccgccc ggcgttcgct tcgcccactc gtggacgtcg taccagtacg
      481 tctccttgag cgcggcggcg agccgctcgt ccgtgtgggc cgggttgaag acgtggtgct
      541 cgaggtcggc gcagaccgcc accgtgtcga gcgagaggtc gggcacctgc gcgagcacct
      601 cggcgggcac ctgggcctcg ccgacggtgt agtgcttgaa gtggaggtcg ggcgcgagct
      661 cgacgaggtc gggccagtcc gggcagctgc gggtgctcac gcggtgagct ccttcctgac
      721 gaggtctgcg agggcggaga tgaaggcggg gtcgtcgttc atggactcga tccggtcgag
      781 ttcgatgccg agcgcggcgg ccctttcgcg cgcctcgacg tcgatgtccc agaggatctc
      841 gaggtggtcg gcgacgaagc cgatcggggc gacgagcacc ttcccgaccc cggcggcggc
      901 gagcgcgtcg aggtggtcga ggatgtccgg cccgagccac ggctcgcccg tcgggctctc
      961 gctctggaac gcgaacgtcc agcgctcgtc cgggatcccg gcgcgctcgg cggcgagccg
     1021 cgcggtcgtc agaagctcgt cgcggtacgg gtcgccctcc gcgatgatcc gctccggaag
     1081 gctgtgggcg ctgaagacga cgtgcccgtc cgtcccgcgc acccggcccg cgatcacctc
     1141 cacgaacggc tcgtgcgcgt gccacgactc gatgaaccga agcgccgcgc gcccgccgag
     1201 cgcgcgctcg agccggtcgc ggtagccgcc gatcgagatg cgggagtagt gcggcgcgag
     1261 cacgagcccg acgagctcct ccgcgccccc ggcgagggcc tgctcgaccg cgtccgcgat
     1321 ccacggcttc cagtgcttca tcccgacgtg gacgggcagc ccgagctcgc cctcgagcgc
     1381 cgcccgctgc cgctccgtca cctcgttgag cggcgagaag ccgatccggc ggtagcgctc
     1441 gacgagctcg tcgactcgct cctgcgacac cggccgcccg ccgcggacgt ccgcgaagta
     1501 ggccgggacg tcctcgggcc ggtccggcga cccgtaggcc atcgcgacga ccgccgtccg
     1561 cgtcacgcgg cgacttccgc ggccgttcgc tcgtggacga gctcgacgag ccggccgagc
     1621 acgtccggat ccgtcgcggg cagcacgccg tgcccgaggt tgaagatgtg ccccggccgc
     1681 ccgccgacga gctcgatgat gcgctgggcg cccgcggccg cgcggttcca cgggccgagc
     1741 aggagcacgg ggtcgaggtt cccctggacg ccgcggtcgg ccccgaccgc ctcccagccg
     1801 cggtcgatcc gcacgcgcca atcgagcccg atcacgtcgc cgcccgtcgc cgccagctcg
     1861 ccgagcaggt gcgtcgcccc cgtgccgaag tggatcgagg gcactccggt ctccgcgacc
     1921 gccgccagga cgcgcgccga gtagggcgcg acgaactccg cgtagtcctc cgccgagagc
     1981 gcgcccaccc aggagtcgaa gagctggatc gcgtccgcgc cggcggcgac cttcgcgcgc
     2041 acgtacgcgg cgaacgtgtc cgcgagcttg tccatgagcc cgtgccagat ctcgggcgcc
     2101 ccgtacatgc agcgcttcgt cttgacgaag tcccgcgacg ggcggccctc cacgaggtag
     2161 ccggcgacgg tgaacgggcc gccgcagaac ccgatcacgg ccccgtccgg cgggagctcg
     2221 ccgcgcacga tccgcacggc ctcgaggagg ggcgcgaacg cctcctccgg atggggcacg
     2281 cgcagcgcct cgacgtccgc cgcgctcgcg accggcgagt cgaccaccgg cccgacgccc
     2341 tcgacgagct cgacctcgat ccccatcccg atcggcggga gcatgatgtc ggcgaacatc
     2401 accgcggcgt ccaccccgtg gcggcgcacc ggctggagcg tcacgtcggc gcagacgtcc
     2461 ggccgctcgc agatctcgaa gagcgtgagc cccttgcgga tctcccggta ctcgggcagc
     2521 gaccggcccg cctggcgcat gaaccagacg ggcgtgcgct ccacgggctc gcggcgggcg
     2581 gcgcggacga ggagcggcgc ggtcacgccg tcaaggctat ccgggcgtct cggagccgct
     2641 gcgggctagg cgtcgtcgcc gacgacgagc gccacgtgcg cgccctggag gtcgcgattc
     2701 gtgatgccgt cgagcggagc gacgcgcggc acgccgaagt ccgccgcgag ccggcgggcc
     2761 tcggcctcgt accccggccg gaacatgacg accgagcgcg cgaagtcggt gcgcggcgcg
     2821 ttcgccgtgc cggcgatgag gtagcccttc gtccgcacga ggtcggcctt ctcgccggcg
     2881 aggccggtcg tgccgttccc gttcaggacg atgaccgagg tctcgccgcg ctcgagggcc
     2941 ggctcggcct tcgcgggctt cgcggccggc tgcgccttcg tcgcctcgcc cttcttcgcg
     3001 ggcgtctccg cggccgccgt gggggacgcc ttctcggcgg ccgccgccgg cgccttgtcg
     3061 tcgccggaga gaccgatgaa gagcaccgcc gcgaggacga ggatgaggag cagctcgagc
     3121 gccgcgatcc cggaggcgac gagcgccgtc gcgcgccacg gatcctggac gttgagatga
     3181 ggatgatcca tcgggtcgct tgatttcgac ccggtctacg gcgttccttc tccccggatc
     3241 gaatcccagg tgcgcgcgat cccgtccgcc ggctcgacct ccgcgcggaa gccgagctcc
     3301 cgctccgcga gcgccgtcga gaggacgctc cgctggagct cgccgaggcg cgcgtcggcg
     3361 aacgccggct cgcgatcgac gccgctcgcc gcccggcagg cgtccgccag ctcgagcacg
     3421 gacgtctcgc ggcccgtccc gacgttgaac acgccgcccg ggttcccgac cgccgcgagc
     3481 gtcgcgcgca cgacgtcctc cacgtagacg tagtcgcgcg tctgcgtccc gtcgccgaag
     3541 atcgtgaacc cctcgcccgc cgcgaggcgc ccgaggaaga tcgccacgac acccgcctcg
     3601 ccgtgcgggt cctgccgggg cccgtagacg ttgccgtagc ggagcgccac gtgcgaggcg
     3661 ccgtagagcc ggcgctccat cgcgaggtac tcctcgcccg cgagcttcga cgtcccgtac
     3721 ggcgagatcg gccgccgcgc ggccgtctcc ggcgccggcg cgtcgcactc gccgtagatc
     3781 gcgccgcccg tcgaggagaa gacgatctgc gcgtcgtgcc gtcgcgccgc ttcgaggatg
     3841 cggatcgtcc cgaggacgtt cacgtccgcg tcgtacgccg ggtcctcgac cgagcgcctg
     3901 acgtccgcct gcgccgcgag gtggaagcag gcgtcgggct cggcctgcgc gaacgcggcg
     3961 cccacggcgt cggcgtcgcg gatgtcgccc tcgacgagcc gcgccccgtc ggcgacccgc
     4021 tcgcgccgtc cggtggagag gttgtcgagc acccagacct cgtccccgcg cgcgagcagc
     4081 gcgtcgacca agtgcgagcc gatgaagccc gcgccgcctg tgaccaccgc ccgcaccggc
     4141 cgtatcctac gaatcccgat gttcgtgacc ttcgaaggcg tcgacggctc cgggaagtcg
     4201 acccaggcgc gactcctcgt cgaggcgctg cgcgcggagg gccgcgaggt cgtcgcgacg
     4261 cgcgagccgg gcggcaccga gctcggtgag cgggtgcgcg acctcctgct gcacgggaac
     4321 ggcctctcgc cgtgggccga ggcgacgctc ttcgccgcgg cgcgcgccca gctcgtcgag
     4381 gaggtgatcc gcccggcgct cgcccgcggg gcggacgtgg tctgcgaccg ctacctcgac
     4441 tcgtcgctcg cgtaccaggg gatcggccgc gagctcggcg tcgaccgcgt gctcgagctg
     4501 aacctcgtcg cgaccggggg gctgcttccg gacaggacgt tcctgctcga cctgcccgtg
     4561 gacgaggcgg cgcgccggcg cggcggcgac tcggaccggc tcgaggccga gggcagggag
     4621 ttcgcggagc gcgtcggaat cgcttaccgt gagctcgcag cggcgttccc gcggcggatc
     4681 gtccgcgtgg acgcgacccg gccacccgag gagcaggcgg agatcgttcg tgcagagctt
     4741 cgagacggtt cctgagcagg acgaggcgaa gcgcctcctc gccgccgcgc tcgcggaggg
     4801 gccggcgcac gcgtacctct tccacgggcc gcccggcgtc gggaagcggc gcgcggcgct
     4861 cgcgttcgcg ggcgagctga tcggcgaccc ggcgcgcgtc gcgcggcgct cgcacccgga
     4921 tctctacgtc ctcgagccgc tcggcgacca gatccggatc gacccggtgc gggacatgcg
     4981 gcgcgacctc cacatgcgcc cgttcgaggc cgagcgccgc gtctacctcg tgcacggcgc
     5041 gcacctgatg aacgaggacg cggccgacgc gctcctcaag gacctcgagg agccgccgcc
     5101 gtacgcggtg atcgtgctcg tcgcggacga gctcgggccg ctgccggaca cgatccgctc
     5161 gcgctgccag ctcgttccgt tccgccggct ctcgcgcggc gcggtgaagt cgtggctcgc
     5221 cgcgcgcggg ctcgagggcg agacgctcga gatcgccgcg cgcgtcgccg ccgggcggct
     5281 cgaccgggcc gagcggctgc tcgacgcagc ggccggcgag cggcggcgcg ccgtgctcga
     5341 cctcgcgcgc gccgtctacc gcgacccggc gttcgagccg ggcgccgcgg cggcggcggt
     5401 gggaaagctg gcggcggcgc gcgcggagga ggcgcgcgcg aaggccgagc gcgaggcggc
     5461 cgccgcggcc gaggccggga tcgaggagcc gaagcgcgag ctcgaccagc ggctccgccg
     5521 cgccgagcgg ggcgccgagc gcgaggaggt cttgaacgcg ctcgacaccc tcgcgggctg
     5581 gtaccgcgac ctcgtggtgg tcggctccgg cgccgaccgc gcggcgctca actccgacct
     5641 cctcgccgag ctcgccgagg acgcgcagcc ggacgtcgcc gtccgcgccg cccgcgccgc
     5701 cgaggccgtc cgcgcgacgt ggcgctcgtt cgagttccag gtctcgaccg ggctcgcgct
     5761 cgaggcgctc ttcgtgcggc tccggcagga gctcgcgtcg ccgctgcccg cggaggccgc
     5821 atgagcgtcg cggtcggcgt cgtcttctcg ccgggcgggc gggtgtactc cttcgacccg
     5881 gacgggctcg agctcgcctg gaacgagcgt gtcgtctgcc agaccgggaa cgggcaggag
     5941 ctcggccggg tcgtcaaggg caaccacgag ctcgagtccc agccgccgac gccgctcaag
     6001 aaggtcatcc ggcgggcgac cgaggtcgac cgcgagcgcg cggccgagaa cgcggaggag
     6061 gcgaaggcgg cgatgcgggt cttccgcgag ctgctccgcc gcttcgggct cgacgtgaag
     6121 ccggtcgcgg tcgagatcgg cctcgacggg tcgcgcgcgg tcgtgtcctt ccaggcggag
     6181 ggacggccga acacgaaggc gctccaggcc gagctcgccc ggcgtctcgg acggcgcatc
     6241 gagctccgct cggtcggccc gcgcgagtgc gcgcggctct gcggcgacca cgggctctgc
     6301 ggcgggatgc agtgctcgcg ccgctacccg acgcacgagc agccgatcac gctgcggatg
     6361 gcgaaggatc aggatctccc ggtcgcgtcc ggccggatca ccgggctctg cgggcggctc
     6421 cgctgctgcc tcgccttcga gcacccgctc tacaagagct tccgcgaccg cgccccgcgc
     6481 gtggggcggc gcgtggagac ccccgcgggc gtcggcctgg tgaccggata cgcggtgctc
     6541 gaggacacgt gcaccgtcgt cctcgacggc gaggagacgc cgacgccgat cccggtcgac
     6601 gactgtcgcg agctcgcggg cgagcgacgc ggcacgaagc gctagcgaac cggaggcgga
     6661 gggaagaccc ctcgtatggc cgtcgagggc acgcacttcg agccgtcgcc gcgcccggac
     6721 tggggcgggc cggagccgtt gacggcggag gactgccgcg cggcgttcga tgccgtcgag
     6781 ccgtacacga tcgggctcga ggaggagctc atggtcgtgt cgccggagac gctcgagctc
     6841 tcggacacgg ccgccgagct cgtcgcgctc gccgccgacg agcggatccg gcccgagctt
     6901 cgcgcgtcgc agctcgagct cgtcacgccg gtctgccgga cggccgtcga ggcgtgcgct
     6961 cacctcggcg agctgcgcgc gcgggtggcg gaggtggcgg cggggacggc gctcctcgcg
     7021 gcggcgggga cgcatccgtt ctccgccggc acgggggcgg tcagcgaggg ggagcgaaac
     7081 gagctgctcg tggaggagta cgagtgggcg gcgctcgaga cgttcgcgtg cgggctgcac
     7141 gtccacgtcg cgctcggcga cgccgaccga gcgcttgcgg tcttcaacgc cctccgctcg
     7201 tacctcccgg agatcgccgc gctcgccgcg aactcgccct tcgcgcgcgg gcgcgacacg
     7261 gggctcgcgt ccatccggcc gaagctcaac caggcgttcc cgcgcgaggg catcccgccc
     7321 gcgttcgcct cgtgggacga gtacgccgac ctcgtccgct ggggccggca cgcgttcccg
     7381 gacccgacgt tcctctggtg ggagctgcgc ccgcatcccg cgttcgggac gctcgaggtc
     7441 cgcgccgccg acgcgcagac gcgcgtggag gacgccgccg cggtcgccgc ggtcgtccag
     7501 tcgctcgtcg tctccctcgg gcggcgccac gacgacgggg agaggctgcc cgccgtctcc
     7561 tcgttccgca tctcggagaa cttctggagc gccctgcggc acgggctctc gggctggatg
     7621 ctcgacctcg agaccggaga gcaggagccg acgctcgagc gcgtcgcacg gctgctggcc
     7681 gagctcgagc ccaccgccgg ggagctcggg ctcgacgcgg agctcgtcca ggcgcgcacc
     7741 ctcctcggcg ggaacggagc cgaccggcag cgctacgtcg cggcgcgcga gggcctcgac
     7801 gggctcgtcc gctggctcgt cgccgagacg gtgctccgct agcgccggcg gagccagagg
     7861 gcgctcagca cggtctgcgc cgcggcgtcg aggaggtaga cgggcgagat ccggcggcgt
     7921 gcgacgtaga cgaggtcgat cgcgccgaag gccgcgggcg cggcgagcga gagcgtctcg
     7981 atctcggggg tgcggcggtt gcgcgcggcc gcaacgccga gcgagagccc gatcgcgagc
     8041 gtgagcgcgc cgaccgtgcg ggcgagccag aagtcggcct tcgggccgct cacgcgctcg
     8101 aacgacccgc ggtcgacgag cggccagatc ccggtcacga cgtagtaggc ggcctggagg
     8161 gccgcgcccg cacgcgcagg gcctagcgtc tcggacgaac cttccacgtc gcgtcggtac
     8221 ccgcacgtgg gcctcggaaa cggctaggcg ccgcgcggaa cgggctacac tgggacggcc
     8281 ccgccgacgt agctcagctg gtagagcact tcactcgtaa tgaaggggtc cccggttcga
     8341 gtccgggcgt cggcttctcc ttaggtcacc cggaagtatc gccacggcga aacgtttccg
     8401 acgtggtcgg cgtcgcccgc gacctcgttc cgggaccggt acgagccccg agatgcggcg
     8461 ttgaagaaag cgtcgcggcg cctgaggtct tcgccggccg gcgtgatgga cgcaa
//
LOCUS       DASTFC010000110         6024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1089747,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000110 DASTFC010000000
VERSION     DASTFC010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089747"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFO88_03275"
     CDS             complement(<1..231)
                     /locus_tag="VFO88_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544573.1"
                     /translation="MEEPLARVEEPLGTGRSSELARRIVENLRRVMHAPDETLDFAVL
                     CLFSEGHLIIEDLPGVGKTTLAKSLARSVDLSF"
     gene            complement(232..1446)
                     /gene="coaBC"
                     /locus_tag="VFO88_03280"
     CDS             complement(232..1446)
                     /gene="coaBC"
                     /locus_tag="VFO88_03280"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET9544574.1"
                     /translation="MGAEDTSGARESAGRTTRVLVGVTGSIAAYKACELVRLLVKAGH
                     DVVPITTPGAERFVARETFEALARRESPRALYPHLLEADLLVVAPLSANTLAKLAHGL
                     ADTVLTETALAFRGPLVLAPAMNVRMWEHPATQANVELLAGRGAHLVGPATGELAEGE
                     TGAGRMVEPEDIATRIGELLGGGGGPLAGRRVLVTAGGTREPLDAVRFVGNRSSGRMG
                     VALAAEAAARGADVTLLAANLAVPAPPGVHTVETPTAADLAREALARGPDADVVLMAA
                     AVADYRPADPSAAKRPKDAEPWAVTLEPTQDVLAELGRRRSNGQVLVGFAADHGERGL
                     ERAREKLAAKNGTLFVFNDVSRDDIGFDSELNEVTVVSESGEHVIGKRDKRAVAAAIL
                     DEVATLLGGGQG"
     gene            complement(1464..1697)
                     /gene="rpoZ"
                     /locus_tag="VFO88_03285"
     CDS             complement(1464..1697)
                     /gene="rpoZ"
                     /locus_tag="VFO88_03285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET9544575.1"
                     /translation="MNHPRIDELLERADSRYGLVIMAAKRARQINNYHHQLGEGTFED
                     VAPPLVESRSKNYLTMALEEIAEGKITYAYDAK"
     gene            complement(1796..2356)
                     /gene="gmk"
                     /locus_tag="VFO88_03290"
     CDS             complement(1796..2356)
                     /gene="gmk"
                     /locus_tag="VFO88_03290"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET9544576.1"
                     /translation="MVTGPSGAGKGTLIRRLVEKRPDLEVAVSATTRRRREGETGGRH
                     YHYLSEEEFDERVAAGEFLEHVVFVSGHRYGTLNSEVERILDSGRSCILELETEGARR
                     VKELRPDALTIFVEPPSFDELERRLRARATESAGEISERLELARRQTLEKDGFDHVVV
                     NDDLERAVAEVEALVRAARVGTSPTA"
     gene            complement(2428..2757)
                     /gene="mihF"
                     /locus_tag="VFO88_03295"
     CDS             complement(2428..2757)
                     /gene="mihF"
                     /locus_tag="VFO88_03295"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HET9544577.1"
                     /translation="MQASRTQVQAPLRSLDQRMEALRRANEIRVRRAQLKKDLKAGSA
                     RIEEILSAPPAYVETAKVIDILMAVPKFGRVKAARFLNQCRISQSKTVGGLSERQRTE
                     LVSLLRR"
     gene            complement(2803..3690)
                     /locus_tag="VFO88_03300"
     CDS             complement(2803..3690)
                     /locus_tag="VFO88_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HET9544578.1"
                     /translation="MILNASGCLDALTAPAVARSLDAFVTKTVTPLPRAGNPPTRIAE
                     TDAGMLNSIGLQNPGIDRFCADVLPRLRELGVPLWVSVGGFAAADYAELCARLDGEPG
                     IAALELNVSCPNVASPVDATEEIVRAARAVTAKPLYTKLSPSAPDVAAAAEAAAAAGT
                     DGLSVVNTLRGLTLDERTLRPVVATGTGGYSGPALRPVALAAVFACHRATGLPIVGMG
                     GVETGRHALDLIAAGASAVALGTVLFRDPSAPGRIRAELAAEAAARGFADPLAAQGVA
                     HAERAAPLPPSTKTPAKTR"
     gene            complement(3687..4331)
                     /locus_tag="VFO88_03305"
     CDS             complement(3687..4331)
                     /locus_tag="VFO88_03305"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544579.1"
                     /translation="MLLRVSRAGLEPGEPGQFFMLSPPGRVLPRPMSLCLALPGELGF
                     LLDPIGPGTRALAALEPGDELDVLGPLGNGFRLDVERPLLVGGGIGIAPLPYLAERLG
                     GAPALLGFRSAGHAEAAALLPGAEVVVEPEYVTERLEPGYDVLACGPEPMLRAVAALA
                     PGAQLAWEAPMACGYGACYGCAVEIGGTLRRLCVEGPVLAAADVAAPLAREEAA"
     gene            complement(4363..>6024)
                     /gene="carB"
                     /locus_tag="VFO88_03310"
     CDS             complement(4363..>6024)
                     /gene="carB"
                     /locus_tag="VFO88_03310"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9544580.1"
                     /translation="LAAGVRPVYRAVDSCAAEVEAASNYLYSTWGERDEGARPREGAS
                     VVILGSGPNRIGQGIEFDYCCVHAARSFRELGYEAVMANCNPETVSTDYDTSDRLYFE
                     PLDVEAVLELCARERPAGVAIQLGGQTPLKLARALEAAGERILGTPFDAIDLAEDRER
                     FGALCDRLGVAVPPWAMATGPEEAAAAAERIGYPVLARPSYVLGGRRMRVCYAAEDVR
                     AAVEPGERILVDRFLEGALELDVDALCDGEETYVAAVMEHVEEAGVHSGDSSCVLPPI
                     SLAPGELARVRDVVRRLAPALGVVGLVNVQLALVRGDAFVLEANPRASRTVPFASKAI
                     GVNLVDAACRLMAGARLADLALPAEGAPSQVSVKAAVFPFARFPGADPVLGPEMRSTG
                     EVMASAGDFPTAFAKAERAAGRPLPAGGRAFLSVADGDKAAAVGLARRLAEVGFTLLA
                     TTGTAAALAQAGIPVTRVRKVSEPGEDATVVDLVRRGRCDLVVNTPAGSGARSDGYRI
                     REAALVARVPCVTTLAGAAAAVEAIAGAGRDEALSLQERVAAGAG"
BASE COUNT          768 a         2229 c         2273 g          754 t
ORIGIN      
        1 gaaggacagg tcgaccgagc gcgcgaggga cttcgcgagc gtcgtcttcc cgacgcccgg
       61 gaggtcctcg atgatcaggt gcccctcgga gaagaggcag aggacggcga agtcgagggt
      121 ctcgtcgggc gcgtgcatga cgcgccggag gttctcgacg atccgccgcg cgagctcgga
      181 cgagcgcccg gtcccgagcg gttcctcgac gcgtgcgagc ggctcttcca cctagccttg
      241 gcctcctccg agcagggtcg cgacctcgtc gaggatagcc gccgcgacag cccgcttgtc
      301 gcgtttgccg atcacgtgct caccgctctc tgagacgacg gtcacctcgt tcagttcgct
      361 gtcgaatccg atgtcgtcac gggatacgtc gttgaagacg aaaagggttc cgttcttcgc
      421 cgcgagcttc tcgcgggcac gctcgaggcc tcgctcgccg tggtcggccg cgaagccgac
      481 cagcacctgg ccgttcgacc tccggcggcc gagctcggcg aggacgtcct gcgtcggctc
      541 gagcgtcacc gcccacggct cggcgtcctt cgggcgcttc gcggcgctcg gatcggcggg
      601 ccggtagtcg gcgacggcgg cggccatgag gacgacgtcc gcgtccgggc cgcgtgcgag
      661 cgcctcgcgc gccaggtcgg cggcggtggg cgtctcgacc gtgtggacgc cgggcggcgc
      721 cgggacggcg aggttcgccg cgagcagggt cacgtcggcg ccgcgcgcgg ccgcctcggc
      781 ggcgagcgcg acgcccatcc ggccggacga gcggttcccg acgaagcgga cggcgtcgag
      841 cggctcgcgc gtgccgcccg cggtcacgag gacgcggcgg ccggcgagcg gccctccgcc
      901 cccgccgagg agctccccga tccgggtggc gatgtcctcc ggctccacca tccgcccggc
      961 gccggtctcg ccctcggcga gctcgcccgt cgcgggcccg acgaggtgcg cgccgcgccc
     1021 ggcgagcagc tcgacgttcg cctgcgtcgc ggggtgctcc cacatgcgga cgttcatcgc
     1081 gggcgcaagg acgagcggcc cgcggaacgc gagcgcggtc tccgtgagga cggtgtccgc
     1141 gaggccgtgc gcgagcttcg cgagcgtgtt cgccgagagc ggggcgacga cgaggaggtc
     1201 ggcctcgagc aggtgtgggt agagcgcgcg cggcgactcg cggcgggcga gcgcctcgaa
     1261 ggtctcgcgc gcgacgaagc gctcggcccc gggcgtcgtg atcgggacga cgtcgtggcc
     1321 cgccttcacg agcagccgga cgagctcgca cgccttgtag gcggcgatcg agccggtcac
     1381 cccgacgagg acgcgcgtcg tccggcccgc ggattcccgc gcgcccgagg tgtcctcagc
     1441 gcccatctga cgtgcccgcg acgctacttc gcgtcgtagg cgtacgtgat cttgccctcg
     1501 gcgatctcct cgagggccat cgtcaggtag ttcttcgagc gggactcgac gagcggaggg
     1561 gcgacgtcct cgaacgtgcc ctcgccgagc tgatggtggt agttgttgat ctgccgggcg
     1621 cgcttggcgg ccatgatcac gaggccgtac cgggagtcgg cgcgctcgag cagctcgtcg
     1681 atgcgtggat ggttcacgcg gatgagtgta ccggccgcgc gcaagccctc ggccgaaggg
     1741 ccgtgtccgc ggcggccacg tccggtgcca ggcacgggac agtggccgcg ggaggctagg
     1801 ccgttggtga cgtgccgacg cgcgcggcgc ggacgagcgc ctcgacctcg gcgacggcgc
     1861 gctcgaggtc gtcgttcacg acgacgtggt cgaacccgtc cttctcgagc gtctggcgcc
     1921 gcgcgagctc gagccgttcg ctgatctctc ccgcgctctc ggtcgcacgt gcgcgcaggc
     1981 gccgctcgag ctcgtcgaag gacggcggct cgacgaagat cgtgagcgcg tccgggcgca
     2041 gctccttcac ccgtcgcgcc ccctcggtct cgagctcgag gatgcagctc cgcccggagt
     2101 cgaggatgcg ctcgacctcg gagttcagcg tcccgtaccg gtgcccggag acgaagacca
     2161 cgtgctcgag gaactcgccc gcggcgaccc gctcgtcgaa ctcctcctcg gagaggtagt
     2221 ggtagtgccg cccgcccgtc tcgccctcgc gccgccggcg cgtcgtcgcc gagaccgcga
     2281 cctcgaggtc gggccgcttc tcgacgagcc ggcggatcag cgtgcccttc cccgccccgg
     2341 acgggccggt gaccacgatg agccggcccc gaagggcgct gccgctcgct ccggaatcct
     2401 ggttcgtctt tatggcgatg gaggtggtca gcggcgtagg aggctcacga gctccgtgcg
     2461 ctggcgctcg gagagcccgc ccacggtctt ggattggctg atccggcact ggttcaggaa
     2521 ccgcgctgcc ttcacgcgcc cgaacttcgg cacggccatg aggatgtcga tcaccttggc
     2581 ggtctccacg taggccgggg gcgcgctcag gatctcctcg atcctcgccg agccggcctt
     2641 caggtccttc ttgagctgtg cgcggcgcac gcggatctcg ttcgcgcggc gcagggcctc
     2701 catccgctgg tcgagggagc ggaggggagc ctggacctgt gtcctcgacg cctgcatggc
     2761 gcgcgattct aacaccatgc ccgtcaacgg caagtatgag gtttagcgcg tttttgcagg
     2821 cgtttttgtc gagggaggca acggcgcggc gcgctccgcg tgggcgacgc cctgcgctgc
     2881 gagcgggtct gcgaacccgc gggcggcggc ttccgcggcc agctcggccc tgatccggcc
     2941 gggcgcggag gggtcgcgga agagcaccgt gccgagcgcc acggcggacg cgcccgcggc
     3001 gatcaggtcg agcgcatggc ggcccgtctc caccccgccc atgccgacga tcgggagccc
     3061 cgtggcccgg tggcaggcga agacggcggc cagcgcgacg ggccggagcg cgggaccgga
     3121 gtaccccccg gtcccggtcg cgacgaccgg gcggagcgtc cgctcgtcga gcgtgaggcc
     3181 gcggagcgtg ttcacgaccg agagcccgtc cgtccccgcg gccgccgccg cctcggccgc
     3241 cgcggccacg tccggcgcgg acggggagag cttcgtgtag agcggcttcg cggtcacggc
     3301 ccgcgcggcc cgcacgatct cctcggtcgc gtcgacgggc gaggcgacgt tcgggcacga
     3361 gacgttcagc tcgagcgcgg cgatccccgg ctcgccgtcg agccgggcgc agagctcggc
     3421 gtagtcggcg gccgcgaacc cgcccacgga gacccagagc ggcacgccga gctcgcgcag
     3481 gcggggaagg acgtcggcgc agaagcggtc gatccccggg ttctggaggc cgatcgagtt
     3541 cagcatcccg gcgtccgtct cggcgatccg cgtcggcggg ttgcccgcgc gcgggagcgg
     3601 cgtgaccgtc ttcgtcacga aggcgtcgag gctccgcgcg acggcgggcg cggtgagcgc
     3661 gtcgaggcag ccggaggcgt tcaggatcac gccgcctcct ctcgcgcgag cggggccgcg
     3721 acgtccgcgg cggcgagcac cggcccttcc acgcagagcc ggcgaagcgt gccgccgatc
     3781 tcgacggcac agccgtagca cgcgccgtag ccgcacgcca tcggcgcctc ccacgcgagc
     3841 tgcgcgccgg gcgcgagcgc ggcgaccgcg cggagcatgg gctccgggcc gcacgcgagg
     3901 acgtcgtagc ccggctcgag ccgttcggtc acgtactccg gctcgacgac gacctcggca
     3961 ccgggcagga gcgccgccgc ctccgcgtgc ccggcggaac ggaagccgag gagcgccggc
     4021 gcgccgccga gccgctcggc gaggtacgga agcggcgcga tcccgatccc gccgccgacg
     4081 aggagcggcc gctcgacgtc gagccggaag ccgttcccga gcggcccgag cacgtcgagc
     4141 tcgtctcccg gctcgagggc ggcgagcgca cgcgtccccg gcccgatcgg gtcgagcagg
     4201 aacccgagct cgccggggag cgcgaggcag aggctcatcg gccgcggcag cacgcgcccg
     4261 ggcggcgaga gcatgaagaa ctggcccggc tcgccgggct cgagccccgc gcgcgagacg
     4321 cgcaggagca cgtagcgccc gacgctctcg acgccgcagg tcctagcccg cgcccgccgc
     4381 aactcgctcc tggagggaca gggcctcgtc gcgccccgcg cccgcgatcg cctcgaccgc
     4441 cgccgccgcg ccggcgagcg tggtcacgca cgggacgcgg gcgacgagcg ccgcctcgcg
     4501 gattcggtag ccgtccgagc gcgcgccgct cccggccggg gtgttgacga cgaggtcgca
     4561 gcggccgcgg cggacgaggt cgacgacggt cgcgtcctcg ccgggctccg agaccttgcg
     4621 gacgcgcgtg accgggatcc cggcctgcgc gagcgcggcc gcagtgcccg tggtcgcgag
     4681 gagcgtgaag ccgacctcgg cgaggcgccg cgcgagcccg accgccgccg ccttgtcgcc
     4741 gtccgcgacg gagaggaacg cgcggccgcc ggcgggcagc gggcgccccg cggcccgctc
     4801 ggccttcgcg aacgcggtcg ggaagtcgcc ggcgctcgcc atcacctcgc cggtcgagcg
     4861 catctcgggc ccgagcaccg ggtcggcccc cgggaagcgc gcgaacggga agacggccgc
     4921 cttgacgctg acctgcgacg gcgcgccctc ggccggcagc gcgaggtcgg cgagccgcgc
     4981 gcccgccatc agacggcacg ccgcgtcgac gaggttcacg ccgatcgcct tcgaggcgaa
     5041 cggcacggtg cgcgacgcgc gcgggttcgc ctcgagcacg aacgcatccc cgcggacgag
     5101 cgcgagctgg acgttcacga gcccgacgac gccgagcgcc ggggcgagcc ggcgaacgac
     5161 gtcgcggacg cgcgccagct cgccgggcgc gagcgagatc ggcgggagca cgcacgacga
     5221 gtcgcccgag tggacccccg cctcctccac gtgctccatg acggccgcga cgtacgtctc
     5281 ctcgccgtcg cagagcgcat cgacgtcgag ctcgagcgcc ccctcgagga agcggtcgac
     5341 gaggatccgc tcgcccggct cgacggccgc gcggacgtcc tccgccgcgt agcagacacg
     5401 catccgccgc ccgccgagca cgtaggaggg ccgggcgagc accgggtagc cgatccgctc
     5461 ggccgcggcg gccgcctcct cgggcccggt cgccatcgcc cagggcggca ccgcgacccc
     5521 gagcctgtcg cagagcgcgc cgaagcgctc gcggtcctcg gcgaggtcga tcgcgtcgaa
     5581 cggcgtcccg aggatccgct cccccgccgc ctcgagcgcc cgggcgagct tgagcggcgt
     5641 ctggccgccg agctggatcg cgaccccggc cggccgctcg cgcgcgcaga gctcgagcac
     5701 ggcctcgacg tcgagcggct cgaagtagag ccggtcggag gtgtcgtagt ccgtcgagac
     5761 ggtctccggg ttgcagttcg ccatgaccgc ctcgtagccg agctcgcgga agctcctcgc
     5821 cgcgtggacg cagcagtagt cgaactcgat tccctggccg atccggttcg ggccggagcc
     5881 gaggatgacg acgctcgcgc cctcgcgcgg ccgcgcgccc tcgtcgcgct cgccccacgt
     5941 ggagtagagg tagttcgagg ccgcctcgac ctcggccgcg caggagtcga ccgcgcggta
     6001 gacgggccgg acgcccgccg cgag
//
LOCUS       DASTFC010000111         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_249007,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000111 DASTFC010000000
VERSION     DASTFC010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249007"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFO88_03315"
     CDS             <1..598
                     /locus_tag="VFO88_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544581.1"
                     /translation="WIDEGIRLMVERAEWARRGLDEVIARLDEFVRRPPAEWPDVQPL
                     PAEAAFEPPPAAAMAGAPTPPEPEPAPEPVAVAEPEPEPEPEAVAEPQPEPEPEPEHE
                     AVADAEPEPEPEPEATAEPHPAEPQPGVPAEPHPPEGPDEPDQEPHPDFDDWEDVPPA
                     PAPRPPEPERESEPEPEQRSAYGDDVPPDRHGWLRRRD"
     gene            602..1042
                     /locus_tag="VFO88_03320"
     CDS             602..1042
                     /locus_tag="VFO88_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9544582.1"
                     /translation="MTGKARLVGLNHVALEVADVDEALEWYGRSFQLTLRSRGRGMAF
                     VDMGDQFLGFSANRRQPPDDHRHFGLVVDDLETARAALQAAGAEILPGRGLMFRDPWG
                     NLVQVVEYAAIQFTKTPRILQGMGLGDLTKSESALAELREKGLG"
     gene            1053..1226
                     /locus_tag="VFO88_03325"
     CDS             1053..1226
                     /locus_tag="VFO88_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544583.1"
                     /translation="MDPGTLTADELARLLREAEQAHGAYEKELGERDEDWPTWYATWI
                     LDRLRERGAGPGP"
     gene            complement(1223..2092)
                     /locus_tag="VFO88_03330"
     CDS             complement(1223..2092)
                     /locus_tag="VFO88_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9544584.1"
                     /translation="MGNESDRDGARLSDWSRLRGSGRRMLGAGVRSFRENDLLSYAAA
                     IAFQVLFALIPLALAAVALLGFLGLEEVWESDIAPQVQERVQEDAFSVIDRLADEILG
                     EKRGLWLTLGAAFALWQVSGAIRTTMSPLNAIYGSDERRSWWQRLLVSFALALAIGPL
                     VIGAALLIQAGPRLVRALELPGVLALLAQVARWGIALALLATAVWLLIRYATADRHPL
                     AWSGVGTVFVVGSWILASLAFGLYATYVADYGSVFGGLATIWVLLTYLYLSSLALLFG
                     IQLDSCVRSEAAG"
     gene            2131..3231
                     /locus_tag="VFO88_03335"
     CDS             2131..3231
                     /locus_tag="VFO88_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9544585.1"
                     /translation="MALMPGRGPREIEEAVPHQLRLAAAIGWRVVVVVVAIGLVGYAL
                     GQVRLVVLPVVFALFIASALIQPVRGLQRLHFPPAAAAATALVVTFVVIGGLGTLLAP
                     HVAGQFGDVRDNVREGTVQVTDWLLEGPLDLSRSDLERYREQALDELEERSGDIAGGV
                     IGGAYLAVEIIAGVLLTFVLAFFFLKDGQRMWPWVVGLFPPLARARVDDVGQIAWATL
                     GGYLRGVTLVALFDAVLIAIALWVIGVPLVLPLALLTFIGGFFPIVGAFTAGAAAALV
                     ALVSNGPVDALLVVAAVVAVQQIEGNVLHPIVVSRAVKIHAVGVLLAVTAGAVIWGIV
                     GAFLAVPVVAVISRAASHLRSGGDEAVEPQPP"
BASE COUNT          478 a         1329 c         1332 g          453 t
ORIGIN      
        1 gtggatcgac gagggcatcc ggctcatggt cgagcgcgcc gagtgggcgc gccgcggcct
       61 cgacgaggtg atcgcgcggc tcgacgagtt cgtccgccgc ccgcccgccg agtggcccga
      121 cgtgcagccg ctccccgccg aagccgcgtt cgagccgccg cccgcggccg cgatggcggg
      181 ggcgccgacg ccgccggagc ccgagcccgc cccggagccc gtggcggtcg cggagcccga
      241 accggagccg gagcccgagg cggtcgcgga gccgcagccg gaaccggaac cggagccgga
      301 gcacgaggcc gtcgccgacg cggagcccga gccggagccg gagcccgagg cgaccgccga
      361 gccccatccg gcggagccgc agccgggcgt cccggccgag ccgcacccgc ccgagggccc
      421 cgacgagccc gaccaggagc cgcatcccga cttcgacgac tgggaggacg tcccgccggc
      481 cccggcgcct cgtccgcccg agcccgagcg cgagtcggag ccggagccgg agcagcgctc
      541 ggcgtacggc gacgacgtgc cgcccgaccg gcacggctgg ctccgccgcc gcgactgagc
      601 cgtgaccggc aaggcgaggc tcgtcgggct gaaccacgtg gcgctcgagg tcgcggacgt
      661 cgacgaggcg ctcgagtggt acggccgctc cttccagctc acgctgcgca gccgcggccg
      721 cggcatggcg ttcgtggaca tgggcgacca gttcctgggg ttctccgcga accggcggca
      781 gcccccggac gaccaccgcc acttcggcct cgtcgtcgac gacctcgaga ccgcccgcgc
      841 cgcgctccag gcggcgggtg ccgagatcct cccggggcgc gggctcatgt tccgcgaccc
      901 gtggggcaat ctcgtccagg tggtcgagta cgccgcgatc cagttcacga agacgccgcg
      961 gatcctccag gggatgggcc tcggcgacct gacgaagtcc gagtccgcgc tcgccgaact
     1021 gcgagagaag ggtctcggat aagctcgcgc ctgtggatcc cggcacgctg accgccgacg
     1081 agctcgcccg gctcctgcgc gaggccgagc aggcgcacgg cgcctacgag aaggagctcg
     1141 gcgagcgcga cgaggactgg cccacgtggt acgccacgtg gatcctcgac cggctccgcg
     1201 agcgcggcgc cggcccgggc ccctagcccg ccgcctcgct gcggacgcac gagtccagct
     1261 ggatcccgaa gagcagcgcg agcgacgaga ggtagagata ggtgaggagc acccagatcg
     1321 tcgcgaggcc gccgaacacg gagccgtagt ccgcgacgta cgtggcgtag aggccgaacg
     1381 cgagcgacgc gaggatccac gagccgacga cgaagacggt gccgaccccg ctccacgcga
     1441 gcggatgccg gtcggccgtc gcgtagcgga tgagcagcca gaccgccgtc gcgaggagcg
     1501 cgagcgcgat tccccagcgc gcgacctggg cgagcagggc gagtacgccc ggcagctcga
     1561 gcgcccggac gagcctcggc ccggcctgga tcaggagcgc tgcgccgatg acgagcgggc
     1621 cgatggcgag cgcgagcgcg aacgagacga gcaggcgctg ccaccaggag cgccgctcgt
     1681 cggagccgta gatcgcgttg agcggcgaca tcgtggtccg gatggcgccg gagacctgcc
     1741 agagcgcgaa cgcggcgccg agcgtgagcc agagcccacg cttctcgccg aggatctcgt
     1801 cggccaggcg gtcgatgacg gagaacgcgt cctcctgcac ccgctcctga acctgcggtg
     1861 cgatgtccga ctcccacacc tcctcgaggc cgaggaaccc gaggagcgcg accgccgcga
     1921 gcgcgagggg gatgagcgcg aacagcacct ggaaggcgat cgcggccgcg tagctcagga
     1981 ggtcgttttc gcggaacgag cgcacgcccg cgccgagcat gcgccggccg ctgccgcgga
     2041 gccggctcca gtcggagagc ctggcgccgt cccggtcgct ctcgttcccc atcgggcaac
     2101 ggtatccggc cgagcgggta tgaacggacg atggcgctga tgcccggccg cggcccgcgg
     2161 gagatcgagg aggcggttcc ccaccagctc cgcctcgcgg cggcgatcgg ctggcgggtg
     2221 gtcgtcgtcg tcgtcgcgat cggcctggtc gggtacgcgc tcggccaggt gcggctcgtc
     2281 gtgctccccg tcgtgttcgc cctcttcatc gcgtccgcgc tcatccagcc cgtgcgcggg
     2341 ctgcagcggc tccacttccc gcccgcggcg gcggcggcga cggcgctcgt cgtgacgttc
     2401 gtcgtgatcg gcgggctcgg cacgctgctc gccccgcacg tcgcagggca gttcggggac
     2461 gtccgcgaca acgtccgcga aggaaccgtg caggtgaccg actggctgct ggaggggccg
     2521 ctcgacctct cgcggagcga cctcgagcgc taccgcgagc aggcgctcga cgagctcgag
     2581 gagcggtcgg gcgacatcgc cggcggggtg atcggcggcg cctacctcgc ggtcgagatc
     2641 atcgccggcg tcctgctcac gttcgtgctc gcgttcttct tcctcaagga cgggcaacgg
     2701 atgtggccgt gggtcgtcgg gctgttcccg cccctcgcgc gggcgcgcgt cgacgacgtg
     2761 gggcagatcg cgtgggcgac gctcggcggc tacctccgcg gggtgacgct cgtcgcgctc
     2821 ttcgacgcgg tgctgatcgc gatcgcgctc tgggtgatcg gcgtcccgct cgtcctgccg
     2881 ctcgcgctcc tcaccttcat cggcgggttc ttcccgatcg tcggggcgtt caccgccggc
     2941 gcagccgcgg cgctcgtcgc gctcgtctcg aacggccccg tggacgcgct gctcgtcgtg
     3001 gccgccgtgg tggcggtgca gcagatcgag gggaacgtcc tccatccgat cgtcgtcagc
     3061 cgggcggtga agatccacgc ggtcggggtg ctcctcgcgg tgaccgcggg cgcggtgatc
     3121 tgggggatcg tcggcgcgtt cctcgccgtg ccggtcgtcg cggtcatctc gcgggccgcg
     3181 tcgcatctcc gctcgggcgg cgacgaggcg gtcgagcccc agccgcccta gcccgggccc
     3241 gaggagccat gtccgcggcg gacgcgcccg gtgcctggca ccggacgtgt cacaagaggc
     3301 gcacgaaggc gcgccgattc gcgcgcgccg gcggattcgg cgtccgccgc cgtctagcct
     3361 cgaaggcatg ccccgagcgc ctcgcatcga ggttcccggc gggatccacc acgtgaccgc
     3421 ccgcggcgtg cgacgcctcc cgctctacac cgtccggacg actaccggct gttcctcgac
     3481 gtcctcggcc tcgtcgccgg gcgcttcggc tggcggatcc tcgcctactg cctcatgccg
     3541 aaccactacc acctcgtcgt cgagacgccg gagccgaacc tgtccgccgg ga
//
LOCUS       DASTFC010000112         6593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_347927,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000112 DASTFC010000000
VERSION     DASTFC010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347927"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFO88_03340"
     CDS             complement(<1..443)
                     /locus_tag="VFO88_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9544586.1"
                     /translation="MSALGYVLSDTVVLAKRSLLRIPRQPDLLLGFTVQPVMFVLLFV
                     YVFGGAIQTPGFDYEDFLIPGILVQSMAFGGFVTALGLAEDLKKGLIDRFRSLPMSRA
                     AVLTGRTTADIATNVIQVAIMLLVGLAVGFRFQTGVPDVIAGIALL"
     gene            complement(440..1405)
                     /locus_tag="VFO88_03345"
     CDS             complement(440..1405)
                     /locus_tag="VFO88_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9544587.1"
                     /translation="MDGPVVETHALEKRFGDTHALRGVDLRVEPGTIFGLLGPNGAGK
                     TTAVRILTTLLRPDAGSARVAGLDVVADADRLRERIGLAGQYAAVDENLTGFENLEMV
                     GRLYHLGRRHSRERSRELLRQFDLADAGDRLVRTYSGGMRRRLDLAAALVARPPVLFL
                     DEPTTGLDIRSRLVLWDAIEALVAEGTTVLLTTQYLDEADHLAGRIAVIDHGLLIAEG
                     TPDELKLQVGGDRLEVRLCSRDDAAAARDALAAVGDGNAEGEDGAVRVAIRERTGAIA
                     EAVRRLDERGIAIDDIAVRTPTLDEVFLTLTGHHAEDEAEEAAER"
     gene            1494..2069
                     /locus_tag="VFO88_03350"
     CDS             1494..2069
                     /locus_tag="VFO88_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544588.1"
                     /translation="MRTRRLAWLASLALMSVGGVIAHAAAYRLVAPELHHHQHTGASA
                     HLQFCVALCATVVLVALGLAFAGRVRRFRALHPPLWLFALLPPVGFVLQEHLERFLHT
                     GSVPVGMSVETVFLVGIVLQLPFALAAYLAARALVTLAVAVVARLRAVYRPRLVSLSL
                     TEPVSVRLHAPRGRLLAGGLGGRAPPVPAVG"
     gene            2114..3823
                     /locus_tag="VFO88_03355"
     CDS             2114..3823
                     /locus_tag="VFO88_03355"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET9544589.1"
                     /translation="MLGCTRRGLVLLVAAFIGLVFASIAFAHSTLIGTTPRADAVEQE
                     SPQEVVLEFNEPVETALGSVRVFNADGERVDLDEIMRPSGPEVAVALQDDLPNGTYTV
                     AWRAISADSDPINGAFVFHVGERGAAAFSGDVGAGVPRTTDVAFTLGRFFSFAFILLC
                     AGGMAALVFPLRSADARVHRRLLGILAVCAGGLVVVALLNILFQGAAAGGLALSDAFS
                     WDVFTAIADTRYGRVALIQAGLALALVGVALFARSRTGGARRTAITVGAVLGGALAFT
                     PTFAGHASTSGNLSIVSDILHVVAASMWVGGLAFVVLALWFALGDRWPLATRAVPRFS
                     TMAVGSVVLLLLGGTINGYLQVRTWSALWETTYGLLLLSKIGLVVPLLALGAYNNRYA
                     VPRLRAGVAEPRERRRFLQAAGVELAIMTAIVAVTAVLVNEMQPRAAGHGGEEGGAMM
                     TAVDFGEFEAEVSVEPGTVGPNEIMVVLGAMEGGEPVEPESVTVSASLPEQEIGPLVY
                     ELEPVEEEEEPAEGDAMTEGGMRYAAEADLALAGEWELRVEIRMNEFDVLTETTMVMI
                     EGR"
     gene            3830..4543
                     /locus_tag="VFO88_03360"
     CDS             3830..4543
                     /locus_tag="VFO88_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HET9544590.1"
                     /translation="MRLLRTLLVTVLAALVAVPAAAAHVTINPAEWEAGGFARFTIRV
                     PSERDVPTTEVTIRFPEAVTSARFMPVPGWERSVEMEQLDEPIQQGEGEPITERLASV
                     TWTGGSIAADEFMEFGVSFRMPDTPGETLVFPAIQTYQGGEVVEWIGPEDSEAPAPTI
                     LITEPAAEEEEEPAEEEPAAEDGEDGEDAGGGEAAPASDTDDDDGSGTATIALVLAIL
                     ALLAGLGALGLGFSRRRTA"
     gene            4702..>6593
                     /locus_tag="VFO88_03365"
     CDS             4702..>6593
                     /locus_tag="VFO88_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544591.1"
                     /translation="MTRPARILSLLAALTALLVLPLAATAKPYDPSATFTTTKNLHQL
                     GFSAQFVAFGGTNFNSDLAFWGDYAYQGKYDGFRIVDVKAPGNPKEILNFTRCVQGTT
                     TGNQGDVVVWDDILVRSWNSPAPAGGAFCGDLFTPEGQEGLHIFDISNPKQPKGLAFV
                     PVEQGSHTATGVPDLANGRLLVYNSASSSLRPGIDIVEIPLDDPASASVIGFEPADRA
                     CHDTAVILGDVMRAACAGHDGFTVWSLGGEEGGSLDDPRFLYSRVVPGVSIGHSASFS
                     WDGKILVFGHEPGGGVQARCQATSADVDKTLFFMDTATGAELGRFVLPRPQSATENCT
                     IHNYNVVPTDKGHILVHGSYQSGIGVVDFTDPTKPEEIAFADPAPLVPAQLGGDWSSY
                     WYNGAIYESDITRGLLVWKLSDDAVAGAKKLSHLNPQTQETSFEVKGKGQKRPPEIVT
                     TGSFELVGHDPLMGRGMNAALAIHGDYAYIGSRTDGKNMNENNAGVFIVDISDPTNPH
                     IVHQMGPPLEGVTRESSRELRVWAEQEILIVLHTNCGTGATHWCSPSGAPGATPTPNN
                     FKFYDISGEFATNPRLIGQLNQSTHEFFLWQDPYDPEYALMFGGSAGNNFAVWDLSPL
                     LEDQLPV"
BASE COUNT          901 a         2403 c         2324 g          965 t
ORIGIN      
        1 aggagggcga tccccgcgat cacgtccggc acgcccgtct ggaaccggaa cccgacggcg
       61 agcccgacga gcagcatgat cgccacctgg atgacgttcg tcgcgatgtc cgccgtcgtc
      121 cggccggtga gcacggccgc ccgcgacatc ggcagcgagc ggaagcggtc gatcaggccc
      181 ttcttcaagt cctcggcgag cccgagcgcc gtcacgaacc cgccgaacgc catcgactgg
      241 acgaggatcc cggggatgag gaagtcctcg tagtcgaagc ccggcgtctg gatcgcgccg
      301 ccgaagacgt acacgaagag gagcacgaac atgaccggct gcaccgtgaa cccgagcagg
      361 aggtcgggct ggcgcggtat ccggagcagg ctccgcttcg cgaggaccac cgtgtccgag
      421 agcacgtacc cgagggcgct caccgctccg cggcctcctc cgcctcgtcc tcggcgtggt
      481 gcccggtcag cgtgaggaag acctcgtcga gggtcggcgt gcgcacggcg atgtcgtcga
      541 tcgcgatccc ccgctcgtcg agccggcgca ccgcctcggc gatcgcgccg gtgcgctcgc
      601 ggatcgcgac gcgcacggcg ccgtcctcgc cctccgcgtt cccgtcgccg accgcggcga
      661 gcgcgtcgcg cgcggcggcg gcgtcgtcgc gggagcagag gcgcacctcg agccggtcgc
      721 cgccgacctg gagcttgagc tcgtccggcg tcccctcggc gatcaggagc ccgtggtcga
      781 tcacggcgat ccgcccggcg agatggtcgg cctcgtcgag gtactgcgtc gtcaggagca
      841 cggtcgtccc ctcggccacg agcgcctcga tcgcgtccca gagcacgagg cggctgcgga
      901 tgtcgagccc cgtcgtcggc tcgtcgagga agagcaccgg cgggcgcgcg acgagcgcag
      961 cggcgaggtc gagccggcgc cgcatcccgc ccgagtacgt gcgcacgagg cggtcgcccg
     1021 cgtcggccag gtcgaactgg cggaggagct cgcgcgagcg ctccctcgag tgccggcggc
     1081 cgaggtggta cagccggccg accatctcga ggttctcgaa gccggtgagg ttctcgtcca
     1141 ccgcggcgta ctggcccgcg aggccgatcc gctcgcgcag ccggtcggcg tccgcgacga
     1201 cgtcgaggcc cgcgacccgc gccgaccccg cgtcgggccg gagcagcgtc gtgaggatcc
     1261 ggacggcggt cgtcttcccg gcgccgttgg gcccgagcag cccgaagatc gtccccggct
     1321 cgacgcggag gtcgaccccg cgcagcgcgt gcgtgtcccc gaagcgcttc tcgagcgcgt
     1381 gcgtctcgac gaccggcccg tccacggcgg agacgctagc gggcgcccga cggccaatgc
     1441 ggcggcaggt tctccaaacc gctcgttgcc gaatgtcgta gcatcgaagc aggatgagga
     1501 cccgcaggct cgcgtggctc gcgtcgctcg ccctcatgtc cgtcgggggc gtgatcgctc
     1561 acgcggccgc gtaccggctc gtcgcgcccg agctgcacca ccaccagcac acgggcgcct
     1621 ccgcgcacct ccagttctgc gtcgcgctct gcgcgaccgt cgtcctcgtc gccctcgggc
     1681 tcgcgttcgc ggggcgcgtg cggcggttcc gcgcgctgca cccgccgctc tggctgttcg
     1741 ccctccttcc gccggtcggc ttcgtgctcc aggagcatct cgagcgcttc ctccacacgg
     1801 gctccgtccc ggtcgggatg tcggtcgaga ccgtcttcct cgtggggatc gtcctccagc
     1861 ttccgttcgc gctcgccgcg tacctcgccg cgcgcgcgct cgtcaccctt gcggtcgcgg
     1921 tcgtcgcgcg cctgcgcgcg gtgtaccggc cgcggctcgt ctcgctttcg ctcaccgagc
     1981 ccgtgtcggt ccgcctgcac gccccgcggg ggcggctgct cgcgggcggc cttggcgggc
     2041 gcgcccctcc ggtcccggcc gtcggctgac cgctgcgccg tcaccaggcg gcgctcaacg
     2101 ggaaggagag ggaatgctcg gctgtacgcg gcgcggcctc gtactgctcg tcgccgcttt
     2161 catcggactc gtcttcgcgt cgatcgcgtt cgcgcactcg acgctcatcg ggacgactcc
     2221 gagggcggac gcggtcgagc aggagtcgcc gcaagaggtc gtgctcgagt tcaacgagcc
     2281 ggttgagacc gcgctcgggt cggtccgcgt cttcaacgcg gacggcgagc gggtggacct
     2341 cgacgagatc atgcgcccga gcggcccgga ggtggccgtc gcgttgcagg acgacctgcc
     2401 gaacggcacc tacacggtgg cctggcgcgc gatctcggcg gactccgatc cgatcaacgg
     2461 ggcattcgtc ttccacgtcg gggaacgcgg ggcggcggcg ttctccggcg acgtcggggc
     2521 aggtgtcccg cggacgacgg acgtcgcgtt cacgctcggg cgtttcttct ccttcgcgtt
     2581 catcctcctc tgcgcgggcg gcatggcggc gctcgtcttt ccgttgcggt cggccgacgc
     2641 ccgcgtccac cggcgcctgc tcggcatcct cgccgtgtgc gccggcgggc tcgtggtcgt
     2701 cgcgctcctg aacatcctgt tccagggcgc ggcggccggc gggctcgcgc tctccgacgc
     2761 gttcagctgg gacgtcttca ccgcgatcgc ggacacgcgg tacgggaggg tcgccctcat
     2821 ccaggcgggc ctcgcgctcg cgctggtcgg ggtcgccctc ttcgcccgga gccgcaccgg
     2881 cggcgcccgg cggacggcga tcacggtggg cgcggtcctc ggcggggcgc tcgcgttcac
     2941 gccgacgttc gcggggcacg ccagcacgtc cggcaacctc tcgatcgtca gcgacatcct
     3001 gcacgtcgtc gcggcctcga tgtgggtcgg tgggctcgcg ttcgtcgtcc ttgcgctctg
     3061 gttcgcgctc ggggaccgct ggccgctcgc cacgcgggcg gttccgcgct tctcgacgat
     3121 ggcggtcggc tccgtcgtcc tcctgctcct cggggggacg atcaacggct acctccaggt
     3181 tcggacctgg agcgcgctct gggagacgac gtacggcctg ctcctcctgt cgaagatcgg
     3241 cctcgtcgtg ccgttgctgg cgctcggcgc ctacaacaac cgctacgcgg tgccgcgcct
     3301 gcgcgcgggc gtggccgagc cgagggagcg gcgccggttc ctccaggcgg cgggcgtcga
     3361 gctcgcgatc atgacggcga tcgtcgccgt caccgccgtc ctcgtgaacg agatgcagcc
     3421 gcgcgccgcc gggcacggcg gcgaggaagg cggcgcgatg atgaccgccg tcgacttcgg
     3481 cgagttcgag gccgaggtct cggtcgagcc gggcaccgtg ggcccgaacg agatcatggt
     3541 cgtcctcggc gcgatggagg gcggcgagcc cgtggagccg gagagcgtca ccgtctcggc
     3601 ctccctgccc gagcaggaga tcggcccgct cgtctacgag ctcgagccgg tcgaggagga
     3661 ggaggagccc gccgaggggg acgccatgac cgagggcggc atgcgctatg cggcggaggc
     3721 cgacctcgcc ctcgcgggcg agtgggagct tcgcgtcgag atccgcatga acgagttcga
     3781 cgtcttgaca gagaccacca tggtcatgat cgaaggaagg taatcaaccg tgagattgct
     3841 tcgcaccctt ctcgtcacgg tcctcgcggc gctcgtcgcc gtcccggccg cggcggcgca
     3901 cgtcacgatc aacccggccg aatgggaggc gggcggcttc gcccggttca ccattcgcgt
     3961 tccgtccgag cgcgacgtcc cgacgaccga ggtgacgatc cggttcccgg aggcggtcac
     4021 gtcggcccgg ttcatgcccg tccccggctg ggagcgttcc gtcgagatgg aacagctcga
     4081 cgagccgatc cagcagggcg agggcgagcc gatcaccgag cggctcgcca gcgtcacgtg
     4141 gacgggcggc tcgatcgccg cggacgagtt catggagttc ggcgtgagct tccgcatgcc
     4201 ggacacgccc ggcgagacgc tcgtcttccc ggcgatccag acgtaccagg gcggtgaggt
     4261 cgtcgaatgg atcggcccgg aggactccga ggcgccggcg ccgacgatcc tcatcaccga
     4321 gccggccgcc gaggaggaag aggagccggc cgaggaagag ccggccgccg aggacggcga
     4381 ggacggcgag gacgccggcg gcggcgaggc cgctcccgcg tccgacacgg acgacgacga
     4441 cggcagcggc accgcgacga tcgcgctcgt gctcgcgatc ctcgcgctgc tcgcgggcct
     4501 cggggcgctc ggcctcgggt tctcgcgccg ccgcaccgcg tagctcgaca cgcagcgccg
     4561 ggggcctccg cgcggggccc ccggccgcgt gactcttgac aactccgcct cgacgggagg
     4621 aaaatcgtcc ccgcttcgca atgcgaagcg cccaggcggg actcccttcc cgcgcgtccg
     4681 tctcctcacc gggaggtcaa cgtgaccagg ccagctcgaa tcctgtcgct ccttgcggcg
     4741 ctgacggctc tgctcgtgct gccgctcgcc gcgacggcga agccctacga tccgtccgcg
     4801 acgttcacga cgacgaagaa cctccaccag ctcgggttct ccgcgcagtt cgtcgcgttc
     4861 ggagggacga acttcaactc cgacctcgcg ttctggggcg actacgccta ccagggcaag
     4921 tacgacgggt tccggatcgt cgacgtgaag gcgcccggga acccgaagga gatcctcaac
     4981 ttcacccggt gcgtgcaggg cacgacgacc gggaaccagg gcgacgtcgt cgtctgggac
     5041 gacatcctcg tccgctcgtg gaactcgccc gcgcctgcgg gcggcgcgtt ctgcggcgac
     5101 ctcttcacgc cggagggtca ggagggcctc cacatcttcg acatctcgaa cccgaagcag
     5161 ccgaagggcc tcgcgttcgt gccggtggag caggggtcgc acaccgcgac cggcgtcccc
     5221 gacctcgcga acgggcggct gctcgtctac aacagcgcgt ccagctcgct ccggccgggg
     5281 atcgacatcg tcgagatccc cctcgacgat cccgcgagcg cgagcgtgat cggcttcgag
     5341 ccggccgacc gcgcgtgcca cgacaccgcc gtgatcctcg gcgacgtcat gcgcgcggcc
     5401 tgcgccggcc acgacgggtt caccgtctgg agcctcggcg gggaggaggg cggctcgctc
     5461 gacgacccga ggttcctcta ctcgcgggtc gtccccggcg tctcgatcgg gcactccgcg
     5521 tcgttctcct gggacgggaa gatcctcgtc ttcggccacg agccgggcgg gggcgtccag
     5581 gcgcgctgcc aggcgacgag cgcggacgtc gacaagacgc tcttcttcat ggacacggcg
     5641 acgggcgccg agctcggccg cttcgtcctt ccgcggccgc agtcggcgac ggagaactgc
     5701 acgatccaca actacaacgt cgtcccgacc gacaagggcc acatcctcgt ccacgggagc
     5761 taccagtcgg ggatcggcgt ggtcgacttc accgacccga ccaagccgga ggagatcgcg
     5821 ttcgccgacc cggccccgct cgtgccggcg cagctcggcg gcgactggtc gtcctactgg
     5881 tacaacggcg cgatctacga gtccgacatc acgcgcgggc tcctcgtctg gaagctctcc
     5941 gacgacgcgg tggcgggggc gaagaagctc tcgcacctga acccgcagac gcaggagacg
     6001 tcgttcgagg tcaagggcaa ggggcagaag cgcccgccgg agatcgtcac gaccggctcc
     6061 ttcgagctcg tcggccacga cccgctcatg ggtcgcggga tgaacgcggc gctcgccatc
     6121 cacggcgact acgcgtacat cggctcccgc acggacggca agaacatgaa cgagaacaac
     6181 gcgggcgtct tcatcgtcga catctccgac ccgacgaacc cgcacatcgt ccaccagatg
     6241 ggcccgccgc tcgagggcgt gacgcgcgag tcctcccgcg agctgcgcgt gtgggccgag
     6301 caggagatcc tgatcgtcct ccacacgaac tgcggcaccg gggcgacgca ctggtgctcg
     6361 ccgtccgggg cgcccggcgc gacgccgacg ccgaacaact tcaagttcta cgacatctcc
     6421 ggggagttcg cgacgaaccc gcggctcatc ggccagctga accagtcgac gcacgagttc
     6481 ttcctctggc aggacccgta cgacccggag tacgcgctca tgttcggcgg aagcgcgggc
     6541 aacaacttcg ccgtgtggga cctctcgccg ctcctcgagg accagctgcc cgt
//
LOCUS       DASTFC010000113         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1633609,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000113 DASTFC010000000
VERSION     DASTFC010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633609"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFO88_03370"
     CDS             <1..1182
                     /locus_tag="VFO88_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544592.1"
                     /translation="MDTTGTDTTETTETTPAEDAAADGETPADDATAGETPTETPAEE
                     TPAAEGDTPADDAAPADSEGDGGSTEPPSEGGGTSGDTGSSGGSSGSAPGQPADGGNG
                     SAGPSGPPTPSGPPVVKPHDHADDDASRRLDPEASAFGGSTVWLHRTLPDPTPPARRL
                     APAFARTLKTEANAAGIGWATVLASIRADGRLGRTPASKAELRTLARTLARLDGDGEW
                     QAFLALRGRTAYADRAQAFARYNRAVGLNALVTGLDAAKASLAKRLLADARISIYGGG
                     RSDIASGKTDVRVVVLLRYLAEAHGQVTVSSLTSGHRIYSRPGVVSAHTYGLAVDIAA
                     LDGRSILGNSAPGGLTEHAVRNILLLPAELRPQQVISLLGMGGPSFPMANHADHIHVG
                     Y"
     gene            1189..1530
                     /locus_tag="VFO88_03375"
     CDS             1189..1530
                     /locus_tag="VFO88_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET9544593.1"
                     /translation="MQLRDERGRVVCEGLTLADGPLTRMKGLLGRKDLPSGEGLLLRP
                     AGSVHTWFMRFPIDAVFLDGEGRVLKIAADLRPWRAAGCRGARAVLELSSGESERRGV
                     RPGDRLVPVEA"
     gene            complement(1664..2614)
                     /locus_tag="VFO88_03380"
     CDS             complement(1664..2614)
                     /locus_tag="VFO88_03380"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9544594.1"
                     /translation="MDAPRDRRRRLVPGRPFLLVNPRSGDGSPTADELVAEANALGVE
                     TELLGEGADAAELARAALARGATALGVAGGDGTLADVAQVAADEDVPFVAVPFGTRNH
                     FARDAGYDREDPLAALPAFTQGVERRVDVGVVCGRLFLNNVSLGVYAAFVHDEAQRTK
                     NRALAGLRMVPAVFGRSRRPLDLSFAVDGRREHHRALVVLVANNDYGFQTLADLEGRE
                     RLDEGRLHAYVIEAAKRRTLLALLWRVLRGRVERAAGWVEYAAESFRVEFHRPRLQAA
                     IDGEPVVLEPPFDFEVRAGALRLLVPPSSRDGEAAADARQ"
     gene            complement(2581..2967)
                     /locus_tag="VFO88_03385"
     CDS             complement(2581..2967)
                     /locus_tag="VFO88_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9544595.1"
                     /translation="MGRVSAIHFYVTDLDRAIDFYTRLLGYGPQAAYDEGENAHSAFF
                     ILEERTYLVLTWDPDRGREVGGSTRLELAADDLQGELAGLQARGLEGRITQTRFGNEV
                     LDVADPDGHLIRFGPAWTLHAIEGAV"
     gene            3133..>3842
                     /locus_tag="VFO88_03390"
     CDS             3133..>3842
                     /locus_tag="VFO88_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET9544596.1"
                     /translation="MSKPFALLVVLAAALAAGCGPVATSGPAELSGRETVKVVATTGM
                     VADVVRRVAGERAEVEQLMGPGVDPHLYKASERDVLALADADAIFFNGLHLEAKLADV
                     LERIEGDVVTQAVTDTMPRERLLSPPAFQGQYDPHVWFDVELWSRTVEQVRDALADLD
                     PTHAATYRANAEDALAELEELHAEVGALAETVPPEKRVLITAHDAFLYFGRAYGFEVR
                     GLQGISTVSEAGAADVNDL"
BASE COUNT          515 a         1467 c         1379 g          481 t
ORIGIN      
        1 atggacacga ccgggaccga cacgacggag acgaccgaga ccaccccggc cgaggacgca
       61 gcggccgacg gggagacgcc cgccgacgat gcgacggccg gcgagacgcc gaccgagacc
      121 cccgccgaag agacgcccgc cgccgagggc gacacgcccg cggacgacgc agcgcccgca
      181 gacagcgagg gtgacggcgg ttcgacggag cccccgtccg agggcggagg cacgtccggc
      241 gacaccggct cctccggcgg cagctcgggc tcggcgcccg gccagccggc cgacggcggg
      301 aacggctccg cgggcccgag cggtccgccc acgccctccg gcccgccggt cgtcaagccg
      361 cacgaccacg cggacgacga cgcgtcccgg aggctcgacc ccgaggcgag cgcgttcggc
      421 gggtcgacgg tgtggctcca ccgcacgctg cccgacccga ccccgcccgc ccgccgcctg
      481 gcgccggcgt tcgcccgcac gctcaaaacg gaggcgaacg cggccgggat cggctgggcc
      541 accgtcctcg cgtccatccg cgcggacggc cggctcgggc gcacgcccgc gtcgaaggcg
      601 gagctccgca cgctcgcgcg cacgctcgcc cgcctcgacg gagacggcga atggcaggcg
      661 ttcctcgcgc tccgcgggcg gacggcctac gccgaccgcg cccaggcgtt tgcgcgctac
      721 aaccgcgcgg tcgggctgaa cgccctcgtc accggcctcg acgccgcgaa ggcctcgctc
      781 gcgaagcgcc tcctcgcgga cgcccgcatc tcgatctacg ggggcggcag gagcgacatc
      841 gcgtcgggca agaccgacgt ccgggtcgtc gtgctcctcc gctacctcgc cgaggcgcac
      901 ggccaggtga cggtctcgag cctcacctcg ggccaccgga tctactcgcg ccccggcgtc
      961 gtctcggcac acacgtacgg gctcgccgtg gacatcgccg cgctcgacgg gcgctcgatc
     1021 ctcggcaact cggcgccggg cgggctcacg gagcacgcgg tgcgcaacat cctcctgctc
     1081 ccggccgagc tccggccgca gcaggtgatc tcgctcctcg ggatgggcgg cccgtccttc
     1141 ccgatggcga accacgccga ccacatccac gtcggctact agccggacgt gcagctacgc
     1201 gacgagcgcg ggcgggtcgt ctgcgagggt ctaaccctcg cggacggccc gctgacgcgg
     1261 atgaagggcc tgctcgggcg gaaagacctg ccgtccggcg aggggctgct gctccggccg
     1321 gccggctcgg tgcacacgtg gttcatgcgc ttcccgatcg acgcggtctt cctcgacggg
     1381 gagggacgcg tcctcaagat cgcggccgat ctccggccct ggcgcgcggc cggttgccgc
     1441 ggagcccgcg ccgttctcga gctctcttcc ggggagtccg aacggcgcgg ggtccgacct
     1501 ggtgaccggc tcgtgccggt cgaggcctag gccccaggga gctagctccg gcccgagagg
     1561 gagatgcgcc gacgcaggtc ggcctcgggg aagttccgga gcttccgctt cagatccgca
     1621 gtcatgtgat gccgcctttc gttgccgctt tggtacgccg cgatcattgt cgggcgtccg
     1681 cggcggcctc cccatccctc gaactgggtg gaacgagcag gcggagcgcc ccggcgcgca
     1741 cctcgaagtc gaagggcggc tcgagcacga ccggctcgcc gtcgatcgcc gcctggaggc
     1801 gcggtcggtg gaactcgacg cggaacgact cggccgcgta ctcgacccag cccgccgcgc
     1861 gctcgacgcg cccgcgcagg acgcgccaga ggagcgcgag gagcgtacgc cgcttcgccg
     1921 cctcgatcac gtaggcgtgc agccgtccct cgtcgagccg ctcgcggccc tcgaggtcgg
     1981 cgagcgtctg gaagccgtag tcgttgttag ccacgagcac gacgagcgcg cggtggtgct
     2041 cgcgccgccc gtcgaccgcg aacgaaaggt cgagcggccg ccgggagcgg ccgaagacgg
     2101 ccggcaccat ccggagccct gcgagcgcac ggttcttcgt ccgctgcgcc tcgtcgtgga
     2161 cgaacgccgc gtagacgccg agcgagacgt tgttcaggaa cagccggccg cagacgacgc
     2221 cgacgtcgac gcgccgctcg accccctgcg tgaacgcggg gagcgccgcg agggggtcct
     2281 cgcggtcgta gccggcgtcg cgggcgaagt ggttccgcgt cccgaagggc acggcgacga
     2341 agggcacgtc ctcgtcggcg gccacctggg ccacgtccgc gagcgtgccg tcgccgcccg
     2401 cgacgccgag cgccgtcgcg ccgcgcgcga gcgcggcccg cgcgagctcc gccgcgtcgg
     2461 cgccctcgcc gagcagctcg gtctcgaccc cgagcgcgtt cgcctcggcg accagctcgt
     2521 ccgcggtcgg cgatccgtcg cccgaccggg gattgacgag gaggaacggc cgcccgggaa
     2581 ctagacggcg ccttcgatcg cgtggagcgt ccacgcgggg ccgaagcgga tcaggtgccc
     2641 gtccggatcg gccacatcca gcacctcgtt cccgaagcgc gtctgcgtga tccgcccctc
     2701 gagcccgcgc gcctggagcc cggccagctc accctggagg tcgtcggccg cgagctcgag
     2761 ccgcgtcgag ccgcccacct cgcgcccgcg gtccgggtcc cacgtgagga cgaggtacgt
     2821 gcgctcctcg aggatgaaga acgccgagtg cgcgttctcg ccctcgtcgt acgccgcctg
     2881 cgggccgtac ccgagcaggc gcgtgtagaa gtcgatcgcg cgatcgaggt cggtgacgta
     2941 gaagtggatc gcgctgacgc ggcccatcta ccgccgccgg cgcgtggccc gcttgtcgcg
     3001 ttccttcttc ttccgctgcc ggtcgttgcg ccagcgcctg atcggtcgag agccgcgtcc
     3061 cattcgctgc ggactctacc gccttcaccc gcggcgcttg cgagcggccc gcgccgaaac
     3121 tacgatttag gcgtgtctaa acctttcgcc ctcctcgtcg ttctcgcggc cgcgctcgcg
     3181 gccggctgcg gccccgtcgc cacgagcggg cccgccgagc tctccggccg cgagacggtc
     3241 aaggtcgtgg cgacgacggg catggtcgcg gacgtcgtcc gccgtgtggc cggcgagcgc
     3301 gccgaggtcg agcagctcat gggccccggc gtcgacccgc acctctacaa ggcgagcgag
     3361 cgcgatgtcc tcgccctcgc cgacgcggac gcgatcttct tcaacggcct ccacctcgag
     3421 gcgaagctcg ccgacgtgct cgagcggatc gagggcgacg tcgtcacgca ggcggtgacc
     3481 gacacgatgc cgcgcgagag gctcctcagc ccgccggcgt tccaggggca gtacgacccg
     3541 cacgtctggt tcgacgtcga gctctggtcg cggacggtcg agcaggtgcg cgacgcgctc
     3601 gcggacctcg acccgacgca cgcggcgacg taccgcgcga acgccgagga cgcgctcgcc
     3661 gagctcgagg agctccacgc cgaggtgggc gcgctcgccg agaccgtccc gccggagaag
     3721 cgcgtcctca tcaccgccca cgacgcgttc ctctacttcg ggcgggcgta cgggttcgag
     3781 gtgcgcgggc tccagggcat ctcgaccgtc tcggaggcgg gcgccgcgga cgtgaacgac
     3841 ct
//
LOCUS       DASTFC010000114         8131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_282169,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000114 DASTFC010000000
VERSION     DASTFC010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282169"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="VFO88_03395"
     CDS             <1..1819
                     /locus_tag="VFO88_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HET9544597.1"
                     /translation="WANLILTNPDMLHIGVLPHHDRWGDVLSNLRYVVVDEAHVYRGV
                     FGSHVANVLRRLRRAAAIYGAEPQFLLASATIANPGEQAAALTGLDVTVVDRDAAPHA
                     ERTVAFWNPPLLDEELGIRASALGEASRILAGLVSNGQRTICFAKSRRAAELIHRMAS
                     DRLDAETAARLSPYRAGYTPAQRREIERRLVDGELLGVTATDALELGIDVGLLDCALS
                     VGFPGTVASLRQQWGRAGRRSHGLAMLVASDDALDQFFMREPEALTGRRVEAAILDHA
                     NPRILDGHVRAAAFEAPLDERDEPTLGPEALERAAALADEGELTRTKAGYVWAGRDYP
                     AARVGLRNTSPDSFAIVEATSGSILGTAERERAYSSLHEGAIYLHLGESYRVLALDLE
                     ARAAVVEPFRSDYYTQAKKETQTAIEEPLAAERRLGLELSFGRVVVTEQVVGYQKKAI
                     SDGATLDLVALELPETTFETEAIWFVPEPEQLAGLEAMPLLLGTLHAAEHSLIALLPL
                     WAMCDRWDIGGLSTNLHFQTGQPTIFVYDGHAGGVGLTRRGFDAFEGWTADTAKLLAG
                     CPCERGCPSCVQSPKCGNLNEPLDKAGALTLLERMLAAR"
     gene            1816..2349
                     /locus_tag="VFO88_03400"
     CDS             1816..2349
                     /locus_tag="VFO88_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9544598.1"
                     /translation="MIPPELRTERLLLRQWRDEDAEPLGAIYAQPEFLEHMPYVDPER
                     QIARFRREWADEGLSLWAAEDLESGRMIGRIGLLRHRDWSLGGDPVEVGWVLDRAFWG
                     RGLATEGGRAALELWRERLLADDELISITTPGNARSLAVMRRLGMTYRGATHWHGYDV
                     VWHAIDRAVDSGPARSF"
     gene            2381..2752
                     /locus_tag="VFO88_03405"
     CDS             2381..2752
                     /locus_tag="VFO88_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9544599.1"
                     /translation="MSQYILLIYGDEAAGNERFAQMSEEEQQADMGRWWAYGQELEQA
                     GAHVAGEALHPTETAQTVTVKNGERLVTDGPFAETKEQLGGFYIVDVASKEEALEWAA
                     KIPSADRGKIEVREIMVFEQP"
     gene            2775..3095
                     /locus_tag="VFO88_03410"
     CDS             2775..3095
                     /locus_tag="VFO88_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9544600.1"
                     /translation="MRYLLLVYAAAEEAAALDTRLALELAERGGLLAAHELEPVTTAT
                     SVRVRGAATLVADGPFAETGERLGGVYVVDLESLDEAIAVAARLPAARDGVVEIRPVV
                     RREG"
     gene            3092..4288
                     /locus_tag="VFO88_03415"
     CDS             3092..4288
                     /locus_tag="VFO88_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9544601.1"
                     /translation="MSSVATVFREEFGRSVAVLARTLGDLDLAEDAVQDAFATAVERW
                     PRDGAPANPGAWILATARNRAIDRIRRDRTLAGKRELLARLEREAPPAPADDDALPDE
                     RLALVFACCHPALAVDAQVALTLRLLGGLTTEEVARAFLVPEATMAQRLVRAKKKVRA
                     AGIPIRVPPPEALPDRLRAVHATLYLVFNQGYGPPPRDELCDEAIRLTRLLAVLLPDE
                     PETLGLAALTLLHDARRAARLDAHGDLVLLEDQDRSLWDEARIAEGRRLLDRALALCR
                     PGPYQVQAAIASLHSDPKPDRRRIAALYDRLAELAPSPVVELNRAVAVALAEGPATGL
                     ALLDALADELDGYHLLHAARADLLRRLGRPGEAAEAYRRALALAPSEPERRFLAGRLH
                     ELPNGG"
     gene            4327..5727
                     /locus_tag="VFO88_03420"
     CDS             4327..5727
                     /locus_tag="VFO88_03420"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET9544602.1"
                     /translation="MLVRPRLAPSIALCGVGAALPAAALFLVGRDVVDASSHLHFTTV
                     GGSALVAAVASIALTVIGARARDGRTVLVGTAFSAMAAMLFLHGLASPGVMVGMNGVV
                     SLSGALTLPLGAAVLALSGFAPFRGPRAIRPLVAIQAALLTGILALGIAGLVDPGLVP
                     SVPEVGSSEALVLVTVTLALFGFLILRSLDTYVLTQRKSDAAVVVGLAFLAAALVGAL
                     HYEYYELGWWLGHGFEIVGLVLVGAAVVLDLRRADQSRPLTGGVPAERLVAAEETFLG
                     TRVRALTVRLAEKDGSTEEHTRRVALLAVQIGERLGLPPSRLRSLATGGLLHDIGKLA
                     VPDEILKKPGSLTDDEFGVIKRHPEWGHHLLGELGDFAPDVRRLVLDHHERLDGSGYP
                     RGLTQAQLDLETRILAVCDVYDALISPRVYRDAWSQERALGLLREGAGALFDPRCVAA
                     LERVLDDAREPLGIAV"
     gene            5750..7009
                     /locus_tag="VFO88_03425"
     CDS             5750..7009
                     /locus_tag="VFO88_03425"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9544603.1"
                     /translation="MARRTARGGLLVVGGGFAGGYVARLVGKRGATLVSDDSFMLYTP
                     ILPEAASGTLEPRHVTVPLRLMCPHAELIIGRASALDEEARVVTVEAESGAWEIEYED
                     LVIAVGAVSRMLPIPGLAEHALPFKDLADAIVLRDHVLRELEGAAAEVDPERAARHLT
                     FVFIGAGYAGVEALAELSDLVRDALRHYPRAVRELPQRWILVDAAERILPEIPRPLGD
                     YAAQELAGRGVELRTSTTLERAEPGCVHLSDGERIEAHTLVWTAGVKANPGLMSLGLP
                     LDERGRIVVGPTLQVEGREHVWALGDCARVPNAATDGTPDPPTCQHALRQARRLAKNL
                     RGKRKAYRYRMLGQVATLGRYRGIADVLGIRLRGFPGWFVTRTYHLYQLPLLSRKLRV
                     VVDWTVALFFRRDIAELGTAARRVSKE"
     gene            complement(7055..>8131)
                     /locus_tag="VFO88_03430"
     CDS             complement(7055..>8131)
                     /locus_tag="VFO88_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HET9544604.1"
                     /translation="PNHPGIRDGDFDFAIIGHEYGHGVSNRLTGGPGINCLTGDERMG
                     EGWSDYIGLVIPLTPELDDPQQPRGLVPYVLYQPDRHGNGLRPRPYTRDMTIQPFTYD
                     SIKSDAWLTGSLATPHGVGHGWNSILWDMTWDLIDKHGFYANLYEGWENGGNTRSMQY
                     VMDGLKIQGCGPSFVVGRDAIITAQEVLTDGEDTCTLWASFSRRGLGFSASDGGSTGR
                     NDGTEAFDTHPDCRRGFTAPVKKTYGELNVVDAGSAVPLRFSDAGLTGLDILMPTNSP
                     FSRQVDCETLAVPSVGERITPREYPIDTETPGSSGLTRSATGVYTYPWKTQEDWAGTC
                     RELVITRLDGVQHRAFFRFVAPES"
BASE COUNT         1022 a         3050 c         2939 g         1120 t
ORIGIN      
        1 gtgggcgaac ctcatcctca cgaacccgga catgctccac atcggcgtcc tcccgcacca
       61 cgaccgctgg ggggacgtcc tgtcgaacct ccgctacgtc gtcgtggacg aggcgcacgt
      121 ctaccgcggc gtgttcggct cgcacgtcgc gaacgtcctg cgccgcctcc gccgggccgc
      181 ggcgatctac ggcgccgagc cgcagttcct gctcgcgtcg gcgacgatcg cgaaccccgg
      241 cgagcaggcc gccgcgctca cggggctcga cgtgaccgtc gtcgaccgcg acgccgcccc
      301 gcacgccgag cggactgtcg cgttctggaa cccgccgctc ctcgacgagg agctcgggat
      361 ccgcgcgagc gcgctcggcg aggcgtcgcg gatcctcgcc ggcctcgtct cgaacggcca
      421 gcgcaccatc tgcttcgcga agagccggcg cgcggccgag ctcatccacc gcatggcgtc
      481 cgaccggctg gacgcggaga cggccgcgcg cctctccccg taccgcgccg gctacacgcc
      541 cgcgcagcgg cgcgagatcg agcggcggct cgtggacggc gagctgctcg gcgtcacggc
      601 gacggacgcg ctcgagctcg ggatcgacgt cggtctcctc gactgcgcgc tctcggtcgg
      661 ctttccgggc accgtggcga gcctcaggca gcaatggggc cgcgccgggc ggcgctcaca
      721 cgggctcgcg atgctcgtcg cctcggacga cgcgctcgac cagttcttca tgcgcgagcc
      781 cgaggcgctg acgggccgcc gcgtcgaggc ggcgatcctc gaccacgcga acccgcgcat
      841 cctggacggg cacgtccgcg cggccgcgtt cgaggcgccg ctcgacgagc gcgacgagcc
      901 gacgctcggc ccggaggcgc tcgagcgcgc cgccgcgctc gccgacgagg gcgagctgac
      961 gcgcacgaag gccggctacg tctgggccgg acgcgactac cccgccgcgc gcgtgggcct
     1021 ccgcaacacg agcccggact cgttcgcgat cgtcgaggcg acgagcggct cgatcctcgg
     1081 cacggcggag cgcgagcgcg cgtactcgtc cctccacgag ggcgcgatct acctccacct
     1141 cggcgagtcg taccgcgtgc tcgcgctcga cctcgaggcg cgcgcggcgg tcgtcgagcc
     1201 gttccgctcc gactactaca cccaggcgaa gaaggagacg cagaccgcga tcgaggagcc
     1261 gctcgcggcc gagcgccggc tcgggctcga gctctccttc ggccgggtcg tcgtgaccga
     1321 gcaggtggtc ggctaccaga agaaggcgat ctcggacggc gcgacgctcg acctcgtcgc
     1381 gctcgagctg cccgagacga cgttcgagac ggaggcgatc tggttcgtcc cggagccgga
     1441 gcagctcgcg gggctcgagg cgatgccgct gctcctcggg acgctccacg ccgccgagca
     1501 ctcgctcatc gccctgctgc ccctctgggc gatgtgcgac cggtgggaca tcggcgggct
     1561 ctcgacgaat ctccacttcc agaccgggca gccgacgatt ttcgtctacg acggccacgc
     1621 gggcggcgtc ggcctcacgc ggcgcggctt cgacgcgttc gagggctgga cggccgacac
     1681 ggcgaagctc ctcgcaggct gcccgtgcga gcgcgggtgc ccgtcctgcg tccagagccc
     1741 caagtgcggg aatttgaacg agccgctcga caaggcgggc gcgctcaccc tgctcgagcg
     1801 gatgctcgcc gcgcggtgat cccgccggag ctccgcaccg agcggctcct gctccggcag
     1861 tggcgcgacg aggacgcgga accgctcggc gcgatctacg cgcagcccga gttcctcgag
     1921 cacatgccgt acgtcgaccc ggagcggcag atcgcgcgct tccgccgcga gtgggccgac
     1981 gaggggctca gcctctgggc ggcggaggac ctggagagcg gccgcatgat cgggcggatc
     2041 ggcctcctgc gccaccgcga ctggtcgctc gggggcgacc cggtggaggt cggctgggtg
     2101 ctcgaccgcg cgttctgggg ccgcgggctc gcgaccgagg gaggccgagc cgcgctcgag
     2161 ctctggcgcg agcggctgct cgcggacgac gagctgatct cgatcacgac gcccgggaac
     2221 gcccgctcgc tcgcggtcat gcgccggctc ggaatgactt accggggcgc cacgcactgg
     2281 cacggctatg acgtcgtctg gcacgcgatc gaccgggctg tcgattccgg cccggcccgt
     2341 tcgttctaga gtcgaacatc catccggaaa ggagctgaac gtgtcccagt acatcctctt
     2401 gatctacggc gacgaggcgg ccggcaacga gcggttcgcg cagatgtccg aggaggagca
     2461 gcaggctgac atgggccgct ggtgggccta tgggcaggag ctcgagcagg cgggcgcgca
     2521 cgtcgccggc gaggcgctcc acccgaccga gaccgcacag accgtgaccg tcaagaacgg
     2581 cgagcgcctc gtgaccgacg gcccgttcgc cgagacgaag gagcagctcg gcggcttcta
     2641 catcgtcgac gtcgcctcga aggaggaggc gctcgagtgg gcggccaaga tcccttcggc
     2701 cgaccgcggg aagatcgagg tccgcgagat catggtcttc gagcagccct agaacgaggc
     2761 cgcgggaggc gcggatgcgc tacctgctcc tcgtctacgc cgccgccgag gaggccgcgg
     2821 cgctcgacac gcggctcgcg ctcgagctcg ccgagcgcgg cggcctcctc gcggcgcacg
     2881 agctcgagcc cgtgacgacc gcgacctcgg tgcgcgtgcg cggggcggcg acgctcgtgg
     2941 ccgacgggcc gttcgccgag acgggcgagc ggctcggcgg cgtctacgtc gtcgacctcg
     3001 agtcgctcga cgaggcgatc gcggtcgcgg cccggcttcc cgccgcgcgc gacggcgtcg
     3061 tggagatccg gccggtcgtc cggagggagg gatgagctcg gtcgcgaccg tcttccgcga
     3121 ggagttcggc cgctcggtcg ccgtgctcgc acggacgctc ggcgacctcg acctcgcgga
     3181 ggacgcggtg caggacgcgt tcgcaaccgc ggtcgagcgc tggccgcggg acggcgcgcc
     3241 cgccaacccg ggcgcctgga tcctcgccac ggcgcgaaac cgggcgatcg accggattcg
     3301 ccgcgaccgc acgctcgcgg gcaagcgcga gctcctcgcc cggctcgagc gcgaggcgcc
     3361 gcccgcccct gccgacgacg acgccctccc ggacgagcgg ctcgcgctcg tcttcgcctg
     3421 ctgccacccc gcgctcgcgg tcgacgcgca ggtcgccctc acgctgcggc tgctcggcgg
     3481 gctcacgacg gaggaggtcg cgcgcgcctt cctcgttccc gaggcgacga tggcgcagcg
     3541 cctcgtccgg gcgaagaaga aggtgcgggc ggcgggaatc ccgatccggg tgccgccccc
     3601 ggaggcgctg cccgaccggc tccgggccgt gcacgcaacg ctctacctcg tcttcaacca
     3661 ggggtacggc ccgccgccgc gcgacgagct ctgcgacgag gcgatccggc tcacacggct
     3721 cctcgccgtg ctgctcccgg acgagccgga gacgctgggc ctcgccgccc tcacgctcct
     3781 ccacgacgcc cgccgggcgg cgcggctcga cgcgcacggc gacctcgtcc tgctcgagga
     3841 ccaggaccgg tcgctctggg acgaggcccg catcgccgaa ggccgccgcc tgctcgaccg
     3901 cgcgctcgca ctctgccggc cggggccgta ccaggtgcag gccgcgatcg cgtcgctcca
     3961 ctccgacccc aagccggacc gccggaggat cgccgcgctc tacgaccggc tcgccgagct
     4021 cgctccgtcc ccggtcgtcg agctgaaccg ggcagtcgcc gtcgcgctcg cggaggggcc
     4081 cgcgacgggg ctcgcgctcc tcgacgcgct cgcggacgag ctcgacggct accacctgct
     4141 ccatgccgcc cgcgccgacc tcctgcgccg gctcggccgg cccggggagg cggcggaggc
     4201 gtaccggcgc gcactcgcgc tggcgccgag cgagccggag cgccgcttcc tcgcggggcg
     4261 cctgcacgag cttccgaacg gggggtaaag gacgcggccc ttcgtgccga tgcgcgcaac
     4321 gtgcccatgc tcgtgcggcc ccggctggca ccctccatcg cgctctgcgg cgtcggcgcc
     4381 gcgctgcccg ccgccgcgct cttcctcgtg ggccgcgacg tggtcgacgc gtcgagccac
     4441 cttcacttca cgaccgtcgg cggaagcgcc ctcgtcgccg cggtcgcctc gatcgccctc
     4501 accgtgatcg gcgcgcgtgc gcgtgacggg cgcacggtcc tcgtcgggac ggcgttctcg
     4561 gccatggccg cgatgctctt cctgcacggg ctcgcctcgc ccggcgtgat ggtcgggatg
     4621 aacggcgtcg tctcgctctc cggcgcgctg acgctcccgc tcggcgcggc ggtgctcgcg
     4681 ctctcgggct tcgcgccctt ccgcgggccg cgcgcgatcc gcccgctcgt cgcgatccag
     4741 gccgcgctcc tgaccgggat cctggccctc gggatcgcgg ggctcgtcga cccggggctc
     4801 gtgccgtccg tcccggaggt cggaagctcc gaggcgctcg tcctcgtcac cgtcacgctc
     4861 gcgctcttcg ggttcctcat cctccgctcg ctcgacacgt acgtcctcac gcagcggaag
     4921 agcgacgcgg cggtcgtcgt cggcctcgcg ttcctcgcgg cggcgctcgt cggggcgctg
     4981 cactacgagt actacgagct gggctggtgg ctcggccacg ggttcgagat cgtcggcctc
     5041 gtcctcgtcg gtgcagcggt cgtcctcgac ctccgccgcg ccgaccagtc gcgcccgctg
     5101 accggcggcg ttcccgccga gcggctcgtc gcggccgagg agaccttcct cggcacccgg
     5161 gtcagggcgc tcacggtccg gctcgcggag aaggacggct ccaccgagga gcacacgcgg
     5221 cgcgtcgcgc tcctggccgt gcagatcggc gagcggctcg gcctcccgcc ctcgcgcctg
     5281 cggagcctcg ccacgggcgg cctcctgcac gacatcggca agctcgcggt gccggacgag
     5341 atcctgaaga agccggggtc gctgacggac gacgagttcg gcgtcatcaa gcgccatccc
     5401 gagtggggcc accacctcct cggcgagctc ggcgacttcg cgcccgacgt gcggcgcctc
     5461 gtgctcgacc accacgagcg cctggacggg agcggctacc cgcgcggcct cacgcaggcg
     5521 cagctcgacc tcgagacgcg gatcctcgcg gtctgcgacg tctacgacgc gctcatctcc
     5581 ccgcgcgtct accgcgacgc gtggtcgcag gagcgcgcgc tcggcctcct ccgcgaaggc
     5641 gcgggcgcgc tcttcgaccc gcgctgcgtc gccgcgctcg agcgcgtgct cgacgacgcg
     5701 cgcgagccac tggggatcgc cgtctagccg aaatcggaag aatggcgccg tggctcggcg
     5761 cacggcacgt ggaggccttc tcgtcgtcgg cggcgggttc gccggcggct acgtcgcgcg
     5821 gctcgtcggc aagcggggcg cgacgctcgt gagcgacgac agcttcatgc tctacacgcc
     5881 gatcctcccg gaggcggcgt ccgggacgct cgagccgcgc cacgtcacgg tgccgctgcg
     5941 gctcatgtgc ccgcacgcgg agctcatcat cggccgcgcg tcggcgctcg acgaggaggc
     6001 gcgcgtcgtc acggtcgagg ccgagtcggg cgcctgggag atcgagtacg aggacctcgt
     6061 gatcgccgtc ggggccgtct cgcggatgct cccgatcccc gggctcgccg agcacgcgct
     6121 gccgttcaag gacctcgccg acgcgatcgt cctgcgcgac cacgtcctgc gcgagctcga
     6181 aggggccgcc gcggaggtgg accccgagcg ggccgcgcgc cacctgacgt tcgtcttcat
     6241 cggggcgggc tacgcgggag tcgaggcgct cgccgagctc tccgacctcg tccgcgacgc
     6301 gcttcgccac tacccgcgcg cggtgcgcga gctgccgcag cggtggatcc tcgtggacgc
     6361 ggcggagcgg atcctcccgg agatcccgcg gccgctcggc gactacgcgg cgcaggagct
     6421 cgcgggacgc ggcgtcgagc tgcgcacctc cacgacgctc gagcgggccg agcccggctg
     6481 cgtgcacctc tcggacggcg agcggatcga ggcgcacacg ctcgtctgga cggccggcgt
     6541 caaggcgaac ccggggctca tgtcgctcgg gctcccgctc gacgagcgcg ggcggatcgt
     6601 cgtcgggccg acgctccagg tggaggggcg cgagcacgtc tgggcgctcg gcgactgcgc
     6661 gcgcgtgccg aacgcggcga cggacggcac gcccgacccg ccgacctgtc agcacgcgct
     6721 ccgccaggcc cggcgcctcg cgaagaacct gcgcggcaag cggaaggcct accgctaccg
     6781 gatgctcggc caggtggcga cgctcggccg ctaccgcggg atcgccgacg tgctgggcat
     6841 ccgcctgcgc ggctttccgg gctggttcgt cacgcggacg taccacctgt accagcttcc
     6901 gctcctctcg cggaagctcc gggtcgtcgt cgactggacg gtcgcgctct tcttccgccg
     6961 cgacatcgcc gagctcggaa cggcggcgcg gcgcgtctcg aaggagtgag aggggagccc
     7021 cggcgagcgg ggctcccctc ccttcgtcgg ctctttagct ctcgggagcg acgaaccgga
     7081 agaacgcgcg atgctggacg ccgtccagcc gcgtgatgac gagctcgcgg caggtaccgg
     7141 cccagtcctc ctgcgtcttc cacgggtacg tgtagacgcc ggtcgcgctc cgcgtgaggc
     7201 ccgagctgcc cggggtctcc gtgtcgatcg ggtactcgcg cggcgtgatc cgctcgccga
     7261 cgctcggcac ggcgagggtc tcgcagtcca cctggcggga gaacggcgag ttggtcggca
     7321 ttaggatgtc gagccccgtg aggccggcgt cgctgaacct gagcggcacc gcgctccccg
     7381 cgtcgacgac gttcagctcc ccgtaggtct tcttcacggg agccgtgaag ccgcggcggc
     7441 agtccggatg cgtgtcgaac gcctccgtgc cgtcgttgcg gccggtcgac ccgccgtcgc
     7501 tcgcgctgaa gccgagcccg cggcgggaga aggacgccca gagcgtgcac gtgtcctcac
     7561 cgtccgtcag gacctcctgc gcggtgatga tcgcgtcgcg cccgacgacg aacgacgggc
     7621 cgcacccctg gatcttgagg ccgtccatca cgtactgcat ggagcgggtg ttgccgccgt
     7681 tctcccaacc ctcgtagagg ttggcgtaga acccgtgctt gtcgatcagg tcccacgtca
     7741 tgtcccagag gatcgagttc cagccgtggc cgaccccgtg cggagtggcc agcgagccgg
     7801 tgagccaggc gtccgacttg atcgagtcgt acgtgaacgg ctggatcgtc atgtcgcgcg
     7861 tgtacggccg cggccgcagg ccgttgccgt ggcgatccgg ctggtagagc acgtacggca
     7921 cgaggccgcg cggctgctgc gggtcgtcga gctccggcgt cagcgggatg acgaggccga
     7981 tgtagtcgct ccagccctcg cccatccgct cgtcgccggt caggcagttg atccccggcc
     8041 cgcccgtcag gcggttcgag acgccgtggc cgtactcgtg gccgatgatg gcgaagtcga
     8101 agtcgccgtc gcggatcccc gggtggttcg g
//
LOCUS       DASTFC010000115         3949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1485229,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000115 DASTFC010000000
VERSION     DASTFC010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485229"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..103
                     /locus_tag="VFO88_03435"
     tRNA            31..103
                     /locus_tag="VFO88_03435"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:His,seq:gtg)
     gene            185..496
                     /locus_tag="VFO88_03440"
     CDS             185..496
                     /locus_tag="VFO88_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544605.1"
                     /translation="MENGTDQPISLFGEELVQDPPLPELLADAQRHLAELHDRYGDVL
                     PAGERFELLSNALDELETSLESVRAARDRLARIETRLTLAFEQAIVRLRSAERELDTA
                     D"
     gene            complement(506..1624)
                     /locus_tag="VFO88_03445"
     CDS             complement(506..1624)
                     /locus_tag="VFO88_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9544606.1"
                     /translation="MTDSITLGRIAGIRIGINWSWLVVFGLIVWTLYAGIFPQSNPGL
                     SDGTYAVMAVVAAILFFLSLLLHELGHALQARHEGMEIEGITLWLFGGVAKFKGMFPS
                     AGAEFRIAIAGPLVSLVLGVGFVLLAWSADFPSSVSGTLMWLGRINLVLLVFNLLPAL
                     PLDGGRVLRSALWYLKGDFASATRLSAGIGRAFGYVFIAAGLFFFFFEGAFSGAWLAF
                     IGWFLLSAAGAEDRALLTRQTLGGLRVRDLMVPEPVTASPDQTIGDFMDHLVWRENHT
                     TYPVAENGRAVGLLPFRCVAAVPRGEWDERRVRDCMLPLTEVPVLGADDELIDAAGDL
                     QESEVRRGLVLDGERLVGLLSLTDVARAFELRRRLRRA"
     gene            complement(1621..1782)
                     /locus_tag="VFO88_03450"
     CDS             complement(1621..1782)
                     /locus_tag="VFO88_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544607.1"
                     /translation="MWKFALLGWKLLPRQHRRALLVQAGKQARRHGPDVARAAGRAYR
                     AARTKDPRP"
     gene            complement(1786..2394)
                     /locus_tag="VFO88_03455"
     CDS             complement(1786..2394)
                     /locus_tag="VFO88_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET9544608.1"
                     /translation="MSELRVLVWSDFICPFCFVGLERAHYLEREHGASVEWLPFDLHP
                     EYPEDGIPRKELEERYGPGVHERTREAVEAAGLEYGPPERIPRSLKALALAELARDRG
                     LYREAHERLMRAYWSEARDLGDDETLVELAVEAGLDADEARTALGDEGYRARVAGSTR
                     AAQEHGVNAVPAFVLDDRLLVLGAHPHEVFDAAVAQLESKVA"
     gene            complement(2424..3476)
                     /locus_tag="VFO88_03460"
     CDS             complement(2424..3476)
                     /locus_tag="VFO88_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544609.1"
                     /translation="MATYERIRDLPLTIDDYELEALSYEISPEFTRHTTVIRLRGDGH
                     EGVGEDVTYDAKDQLDLQAEGPTLPLAGSHTIDSFSQLVDRLALFPDGPSRDDWVNYR
                     RWAYESAALDLALRQAGTTLAAAVGREPRPVTFVVSMNLGQPPTTARVRSILEHYPGT
                     RFKLDARSDWTDEIFDELAALGVVDSIDLKGAYTGTPVDQGPDPVLYRRVAETFPDAW
                     IEDPALTGETDPVLAPYRDRITWDAPIHSVGDIEGLPFPPKTVNVKPSRFGSLEELCA
                     AYDYCEERGIGAYGGGQFELGPGRGQIQYLASLFHPDGPNDTAPGGFNETSPKPGLPT
                     SPLEPRPEATGFRWAG"
     gene            3564..>3949
                     /locus_tag="VFO88_03465"
     CDS             3564..>3949
                     /locus_tag="VFO88_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9544610.1"
                     /translation="MANVESSIDVNVPVTTAYNQWTQFEEFPEFMEGVEYVHQIDDTH
                     LRWAAEIGGEREEWEAEITEQTPDQRIAWTSTTGKHNAGVVTFHKIDDMTTRVMLQMD
                     WDPEGLKEKAGSMLGRDEAQIKADLER"
BASE COUNT          593 a         1385 c         1445 g          526 t
ORIGIN      
        1 tcggccgcgc gataccattg accaggcgcg gtggcggtag ctcagctggt agagccccgg
       61 gttgtggtcc cggtggtcgc gggttcgaat cccgtccgcc accctctcgc gccgccgcgc
      121 ccgaccgcgg cgggcgccgg cgacggacgc ggagccccgc acgctgcgag tagcgttcgg
      181 gcggatggag aacggaaccg accagccgat ctcgctcttc ggggaggagc tcgtgcagga
      241 tccgccgctg ccggagctcc tcgcggacgc gcagcgccat ctcgccgagc tgcacgaccg
      301 ttacggggac gtcctgccgg cgggcgagcg gttcgagctg ctctcgaacg cgctcgacga
      361 gctcgagacg tcgctcgaaa gcgtccgcgc ggcccgcgac cggctcgcga ggatcgagac
      421 ccgcctgacg ctcgcattcg agcaggcgat cgtccggctc cgctcggccg agcgcgagct
      481 cgacacggcg gactgacggc ggggcctagg cccgccgcag ccggcggcgc agctcgaacg
      541 cgcgggccac gtccgtgagg gagaggagcc cgacgagccg ctcgccgtcg aggacgagcc
      601 cgcgccgcac ctcgctctcc tggaggtcgc cggccgcgtc gatcagctcg tcgtccgcgc
      661 cgagcaccgg cacctccgtg agcggcagca tgcagtcgcg cacccgccgc tcgtcccatt
      721 cgccgcgcgg caccgcggcc acgcagcgga acgggagaag cccgaccgcc cgcccgttct
      781 cggcgaccgg gtacgtcgtg tggttctcgc gccagacgag gtgatccatg aagtcgccga
      841 tcgtctggtc cggcgacgcc gtgaccggct cggggaccat gaggtcgcgc acgcgcaggc
      901 cgccgagcgt ctgccgggtg aggagcgcgc gatcctcggc gccggccgcc gacaggagga
      961 accagccgat gaacgcgagc cacgcgccgc tgaacgcccc ttcgaagaag aagaagaaca
     1021 gcccggcggc gatgaagacg tagccgaagg cgcgcccgat ccccgcggag aggcgcgtcg
     1081 ccgacgcgaa gtcgcccttg aggtaccaga gcgcagagcg aagcacgcgg ccgccgtcga
     1141 ggggcagcgc ggggaggagg ttgaagacga gcagcacgag gttgatccgg ccgagccaca
     1201 tgagcgtccc gctgaccgag ctcgggaagt ccgcgctcca cgcgagcagc acgaacccga
     1261 cgccgagcac gagcgagacg agcggccccg cgatcgcgat tcggaactcc gcgccggccg
     1321 acgggaacat ccccttgaac ttcgcgacgc cgccgaagag ccagagcgtg atcccctcga
     1381 tctccatccc ctcgtgtcgc gcctggagcg cgtggccgag ctcgtgcagg aggagcgaga
     1441 ggaagaacag gatcgccgcg acgaccgcca tgaccgcata cgtgccgtcg ctgagccccg
     1501 ggttcgactg cgggaagatg ccggcgtaga gcgtccagac gatcagcccg aagacgacga
     1561 gccagctcca gttgatcccg atgcggatcc cggcgatccg cccgagcgtg atgctgtcgg
     1621 tcacggccgc gggtctttgg tgcgcgcggc gcggtaggcg cggcccgctg cgcgggcgac
     1681 gtccggaccg tgccgcctcg cctgcttgcc cgcctggacg agcagggcgc gccggtgctg
     1741 cctggggagg agcttccagc ccaagagggc gaacttccac atcgttcagg ccaccttact
     1801 ctcgagttgg gcgaccgccg cgtcgaagac ctcgtgcggg tgggcgccga ggacgagcag
     1861 ccggtcgtcg aggacgaacg cgggcaccgc gttcacgccg tgctcctgcg ccgcgcgggt
     1921 cgaccctgcg acgcgcgcgc ggtagccctc gtcgccgagc gcggtccgcg cctcatcggc
     1981 gtcgagcccg gcctcgacgg cgagctcgac gagcgtctcg tcgtcgccga ggtcgcgcgc
     2041 ctccgaccag tacgcgcgca tgagccgctc gtgcgcctcg cggtagaggc cgcggtcgcg
     2101 ggcgagctcg gcgagcgcga gtgccttcag cgagcgcggg atccgctccg gcgggccgta
     2161 ctcgagcccg gccgcctcga ccgcctcgcg cgtgcgctcg tggacgccgg gcccgtagcg
     2221 ctcctcgagc tccttccgcg ggatgccgtc ctccgggtac tccggatgga ggtcgaacgg
     2281 aagccactcg acgctcgccc cgtgctcgcg ctcgaggtag tgcgcccgct cgagcccgac
     2341 gaagcagaac gggcagatga agtcgctcca gacgaggacg cgcagctcgc tcatcgcctt
     2401 cccgttcccg ccggccgcca tcgctatccc gcccagcgga acccggtcgc ctccggccgc
     2461 ggctcgagcg ggctcgtcgg gaggcccggc ttcggggacg tctcgttaaa cccgcccggc
     2521 gcggtgtcgt tcggcccgtc cgggtggaag agcgaggcga ggtactgaat ctgcccgcgc
     2581 cccggcccca gctcgaactg cccgccgccg tacgcgccga tcccgcgctc ctcgcagtag
     2641 tcgtacgcgg cgcagagctc ctcgaggctg ccgaagcgcg agggcttcac gttgaccgtc
     2701 ttcggcggga agggcaggcc ctcgatgtcg ccgaccgagt ggatcggcgc gtcccacgtg
     2761 atccggtcgc ggtagggcgc gagcacgggg tccgtctcgc cggtgagcgc cgggtcctcg
     2821 atccacgcgt ccgggaaggt ctccgcgacg cgccggtaga ggacgggatc cgggccctgg
     2881 tcgaccggcg tgccggtgta cgcgcccttc aggtcgatcg agtcgacgac gccgagcgcg
     2941 gccagctcgt cgaagatctc gtccgtccag tcgctgcgcg cgtcgagctt gaagcgcgtg
     3001 ccggggtagt gctcgaggat cgagcggacg cgcgcggtcg tgggcggctg gccgaggttc
     3061 atcgagacga cgaacgtgac gggcctcggc tcccggccga ccgcggcggc gagcgtcgtc
     3121 cccgcctgac ggagcgcgag gtcgagcgcc gcgctctcgt acgcccagcg ccggtagttc
     3181 acccagtcgt cgcgcgacgg cccgtcgggg aagagcgcga gccggtcgac gagctgcgag
     3241 aaggagtcga tcgtgtgcga gccggcgagc gggagcgtcg gcccctccgc ctgcaggtcg
     3301 agctggtcct tcgcgtcgta cgtcacgtcc tcgccgacgc cctcgtgtcc gtcgccgcgc
     3361 aggcggatga cggtcgtgtg ccgcgtgaac tcgggcgaga tctcgtagct cagcgcctcg
     3421 agctcgtagt cgtcgatcgt gagcggcagg tcgcggatgc gctcgtaggt ggccatggcg
     3481 cgcagtctgc cttctcgggt ttgcgccgtg tagcggtggg aacccggtgg tgagcgattt
     3541 tccgagacga caggaggtag gagatggcga acgtcgagtc ttcgatcgac gtcaacgtgc
     3601 cggtgacgac ggcatacaac cagtggactc agttcgagga gttcccggag ttcatggagg
     3661 gcgtcgagta cgtccaccag atcgacgaca cccatctgcg ctgggcggcc gagatcggcg
     3721 gcgagcgcga ggagtgggag gcggagatca ccgagcagac gccggaccag cggatcgcct
     3781 ggacgtcgac gaccggcaag cacaacgccg gcgtcgtcac gttccacaag atcgacgaca
     3841 tgaccacccg ggtcatgctc cagatggact gggatccgga aggcttgaag gagaaggccg
     3901 gctcgatgct cggccgcgac gaggcccaga tcaaggcgga tctcgagcg
//
LOCUS       DASTFC010000116         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1271062,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000116 DASTFC010000000
VERSION     DASTFC010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271062"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFO88_03470"
     CDS             <1..1176
                     /locus_tag="VFO88_03470"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9544611.1"
                     /translation="VAVALAGFSVGEAEGLRRAMSRKRSEAAIEAYRDRFLEGAAGRG
                     VPRETAELVFGKINGFASFGFPKSHAAAFALLAYQSAWLRHRYPAEFLCALLNAQPMG
                     FYPPASLVRDAQRRGVEVRPPDVRVSEAKCRLEGEAVRVGLGYVRGLGEEPAKALAAE
                     RERRGPFTGIRDLAQRAPVDRPALEALVAGGACDSFGWPRRQLLWRLGLAPRSVSVGE
                     GGEERQLALPLDPQTDVPELPEQTSWERMLADYRVTGLSVGVHPLTLLRPALPEGVTR
                     SDELGGLAHGAPVAVAGMVIARQRPATANGVVFMLLEDEEGQVNLVVPPPVYERCRAA
                     VRGEPLVLARGRFERYERNRNVVVESLESLSALARAAAGEVEVSESMPRAHHFGGRR"
     gene            1320..1586
                     /locus_tag="VFO88_03475"
     CDS             1320..1586
                     /locus_tag="VFO88_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544612.1"
                     /translation="MGLLDWFRRRGAPGAGASEADADAHPVTTSEPETATEAATEAGH
                     GAGVPAGSPAEGPPVGVSDPGSITGEDEPIAAGEGDELEPPQGP"
     gene            complement(1593..2483)
                     /locus_tag="VFO88_03480"
     CDS             complement(1593..2483)
                     /locus_tag="VFO88_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544613.1"
                     /translation="MRFPGLLGLAFSLLVVFAVASPAAAQPAGSVVFSASRDGDHELY
                     RVAAAGGPVTRLTRNRSSDAAPAVSPNGRLVAFVSDRDGDEELFVIRLDRTALWQVTR
                     NRAADHSPAWSPNGRHLVFASDRSGDFELYAAGRYGGDVRRLTRSPGFDVQPAWSPDG
                     RRIAFASTRVGGQFDVYSMRRDGSDVRRLTFEAGTEEEPVADMAPAWSPDGARIAFSS
                     TRGGVIDDLWTMGADGSGLVQVTATAFVDEWTPRFSPDGEWLLFTASSLAGGVRIAVA
                     RVDGSERRRLAGGLFASWAP"
     gene            complement(2555..>4111)
                     /locus_tag="VFO88_03485"
     CDS             complement(2555..>4111)
                     /locus_tag="VFO88_03485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9544614.1"
                     /translation="LLGLSAVAAALGLLAIPSQWFPVDDAAGSVILLIGLAVGVDYSL
                     FYLKREREERAAGRGPEAALEAAAATSGRAVLISGLTVIVALAGLFLGGTKFWNSIAI
                     GTILVVAIAVAGSLTVLPAVLASLGDRVEKGRLPLIGRRSHADTSRVWDAILTRVLRR
                     PLVSAVAAGGFLLVLAAPTLGLHTSLPGASSLPKDLPILQTYDRIQTAFPGGPLPAIV
                     AVEAENVTAPEVQAAIADLRERALATGVMADPITTQVSPNGRVALVSVPLAGEGAGDP
                     VSEQALAALRDDVVPASLGAAGLEAGVTGETAVSKDFNDLVKERAPFVFGFVLLLAFL
                     LLLVSFRSIVIAGKAIVLNLLSVGAAYGLLVVVFQHGWGESLLGFESTGAITSWLPLF
                     MFVILFGLSMDYHVFILSRVREAFDRGLSTEEAVAHGIKTTAGVVSAAAFVMVAVFSI
                     FVTLSTVEMKQAGFGLATAIAIDATIVRAVLLPSLMKLLGDWNWYLPRRLEWLPQVSH
                     ETEAAPVPAS"
BASE COUNT          570 a         1492 c         1582 g          467 t
ORIGIN      
        1 gtcgccgtcg cgctcgccgg gttctccgtc ggcgaggcgg aggggctgcg ccgcgcgatg
       61 agccgcaagc ggtcggaggc ggcgatcgag gcgtaccgcg accggttcct cgagggcgcg
      121 gccgggcggg gcgtcccgcg cgagacggcg gagctcgtct tcgggaagat caacgggttc
      181 gcgtcgttcg ggttcccgaa gtcgcacgcg gccgcgttcg cgctcctcgc ctaccagtcg
      241 gcctggctcc ggcaccggta tccggcggag ttcctctgcg cgctcctgaa cgcgcagccg
      301 atggggttct acccgccggc ctcgctcgtg cgggacgcgc agcggcgcgg ggtggaggtg
      361 cgcccgccgg acgtgcgcgt gtcggaggcg aagtgccggc tcgagggcga ggcggtgcgc
      421 gtcgggctcg ggtacgtgcg cgggctgggg gaggagccgg cgaaggcgct cgccgccgag
      481 cgggagcggc gcgggccgtt cacggggatc cgcgacctcg ctcagcgggc gccggtcgac
      541 cggccggcgc tcgaggcgct cgtcgcgggc ggcgcgtgcg actcgttcgg ctggccgcgg
      601 cggcagctcc tctggcggct cggcctcgcg ccccgctcgg tctcggtggg tgagggcgga
      661 gaggagcgcc agctcgcgct cccgctcgac ccgcagacgg acgtcccgga gctgccggag
      721 cagacgtcgt gggagcggat gctcgccgac taccgcgtga ccgggctctc ggtcggcgtg
      781 cacccgctca cgctcctgcg gccggcgctg ccggagggcg tgacgcggag cgacgagctc
      841 ggcgggctcg cgcacggcgc cccggtggcc gtcgccggga tggtgatcgc acgccagcgg
      901 cccgcgacgg cgaacggcgt cgtgttcatg ctgctcgagg acgaggaggg ccaggtgaac
      961 ctcgtcgtcc cgccgcccgt gtacgagcgc tgccgggccg cggtgcgcgg cgagccgctc
     1021 gtcctcgcgc gcggccgctt cgagcgctac gagcggaacc gcaacgtcgt cgtcgagtcg
     1081 ctcgagagcc tctccgccct cgcccgcgcc gcggccgggg aggtcgaggt gagcgagtcg
     1141 atgccgcgcg cccaccactt cggcgggcgc cgctgacacg cttccggcac ggaatcgtga
     1201 ccatgcgtgc gcttctcgcc gctagcgtga ccgatagggg gtaaccagag ggggtccccc
     1261 gggggaatca catccccggc ttccgcgcgc cgagccggtt cgcgagtaga gtcaccgcca
     1321 tgggactcct cgactggttc cgccgccgcg gcgctccggg agcgggcgcg tccgaggccg
     1381 acgccgacgc gcacccggtc acgacctccg agcccgagac ggcgacggag gcggcgacgg
     1441 aggccggtca cggcgccggc gtcccggccg ggtctccggc cgaggggccg ccggtcggcg
     1501 tgagcgaccc cggctccatc acgggcgagg acgagccgat cgccgccggc gagggcgacg
     1561 agctcgagcc gccgcagggc ccgtagccgg cgctacggcg cccaggacgc gaacagcccg
     1621 cccgcgagcc gccgccgctc cgagccgtcc acccgcgcga ccgcgatccg caccccgccc
     1681 gcgagcgacg acgccgtgaa gaggagccac tcgccgtcag gcgagaagcg cggcgtccac
     1741 tcgtccacga acgcggtcgc ggtcacctgc acgagcccgg atccgtcggc gcccatcgtc
     1801 cagaggtcgt cgatcacgcc cccgcgcgtc gacgagaacg cgatccgtgc gccgtcgggc
     1861 gaccaggcgg gcgccatgtc ggcgaccggc tcctcctccg tgccggcctc gaaggtcagc
     1921 cgccggacgt cggagccgtc gcgcctcatc gagtacacgt cgaactgccc gcccacgcgc
     1981 gtcgacgcga acgcgatccg gcggccgtcc ggcgaccacg ccggctggac gtcgaacccc
     2041 gggctccgcg tcagccggcg gacgtcgccg ccgtagcggc cggccgcgta gagctcgaag
     2101 tcgccggagc ggtcggaggc gaagacgaga tgccgcccgt tcggcgacca cgccggggag
     2161 tggtcggccg cgcggttgcg tgtcacctgc cagagcgcgg tgcggtcgag ccgtatgacg
     2221 aagagctcct cgtcgccgtc ccggtcgctc acgaacgcga cgagccggcc gttcggcgag
     2281 accgcgggcg ccgcgtcgct cgagcggttc cgcgtgaggc gcgtgacggg gccgccggcc
     2341 gccgccacgc ggtagagctc gtggtcgccg tcccggctcg cggagaagac gaccgacccc
     2401 gccggctgag ccgccgcggg cgaggcgacc gcgaagacga cgagcagcga gaaggcgagg
     2461 ccgaggagtc cggggaagcg catgtgaacc actcctttcg tcgcgcgggc ggccccgccg
     2521 ttacgcgggg ccgccgcacg cttgcgtgat cgcgtcagct cgcggggacc ggcgcagcct
     2581 cggtttcgtg cgagacctgc ggcagccact cgagccggcg cgggaggtac cagttccagt
     2641 cgcccaggag cttcatgagc gacggaagga gcacggcccg gacgatcgtc gcgtcgatcg
     2701 cgatcgccgt cgcgagcccg aagcccgcct gcttcatctc gacggtcgag agcgtcacga
     2761 agatcgagaa caccgcgacc atcacgaacg ccgcggcgct cacgacgccg gccgtcgtct
     2821 tgatcccgtg cgcgaccgct tcctcggtcg agagaccgcg gtcgaacgcc tcgcgcacgc
     2881 ggctcaggat gaacacgtgg tagtccatcg agagcccgaa caggatcacg aacatgaaga
     2941 gcggcagcca cgacgtgatc gcgccggtcg actcgaagcc caggaggctc tcgccccagc
     3001 cgtgctggaa gacgacgacg aggagcccgt aggccgcgcc gaccgacagc aggttgagga
     3061 cgatcgcctt gcccgcgatc acgatcgacc ggaacgagac gagcaggagc aggaacgcga
     3121 ggaggaggac gaacccgaag acgaacgggg cccgctcctt cacgaggtcg ttgaagtcct
     3181 tcgagacggc cgtctcgccg gtcacgccgg cttcgagccc ggccgcgccg agcgaggccg
     3241 ggacgacgtc gtcgcggagc gcggcgagcg cctgctccga gaccgggtcg ccggcgccct
     3301 cgcccgcgag cgggacggac acgagcgcga cgcggccgtt cgggctcacc tgcgtcgtga
     3361 tcgggtcggc catcacgccg gtcgcgagcg cccgctcgcg caggtcggcg atcgccgcct
     3421 gcacctccgg cgcggtcacg ttctcggcct cgacggcgac gatcgccggc agcgggccgc
     3481 cggggaacgc cgtctggatc cggtcgtacg tctggaggat cggcaggtcc ttcggcagcg
     3541 acgacgcgcc ggggagcgac gtgtgcagcc cgagcgtcgg cgccgcgagg acgagcagga
     3601 agccgccggc ggcgacggcg gagacgagcg gccggcgcag gacgcgcgtc aggatcgcgt
     3661 cccacacgcg cgaggtgtcc gcgtggctcc ggcgcccgat cagcggcagg cggcccttct
     3721 cgacccggtc gccgagggag gcgagcacgg ccgggaggac cgtcagcgag ccggcgacgg
     3781 cgatcgcgac gacgaggatc gtgccgatcg cgatcgagtt ccagaacttc gtcccgccga
     3841 ggaagagccc cgcgagcgcg acgatcacgg tcaggccgga gatgaggacg gcgcggcccg
     3901 aggttgcggc ggccgcctcg agcgcggcct ccgggcccct gccggccgcc cgctcctccc
     3961 gttcgcgctt gaggtagaag agcgagtagt cgacgccgac ggcgaggccg atcaggagga
     4021 tgaccgagcc ggccgcgtcg tcgaccggga accactggct cgggatcgcc aggagcccga
     4081 gcgcggccgc gacggcggag agcccgagca g
//
LOCUS       DASTFC010000117         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1023732,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000117 DASTFC010000000
VERSION     DASTFC010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023732"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /gene="tsaB"
                     /locus_tag="VFO88_03490"
     CDS             <1..568
                     /gene="tsaB"
                     /locus_tag="VFO88_03490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET9544615.1"
                     /translation="LRDGDVLGERRTKAARLLADAEELCAAAGIQPAELDRLVAGTGP
                     GGYTGLRMGLVTARVLAFSLGLDAAGVSTLDALAAGAPGAEPLLDARRGQVFARGMNG
                     EPRVLAPEDLEVEPGAVFVGDGAVRYRELVEARGGVVPADDDERHVPWARHHAGLARD
                     FGPAELLEPLYLREPDAERSLREGRLRT"
     gene            565..1059
                     /gene="rimI"
                     /locus_tag="VFO88_03495"
     CDS             565..1059
                     /gene="rimI"
                     /locus_tag="VFO88_03495"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET9544616.1"
                     /translation="MTIELRRLTLGDLGAIERIEREAYPTPWSRSMFAGEVAKPSSIS
                     LGAFDLDGEETLVGYLIISRYVDAWHVMNIAVAHAYRRRGIAIRMLEELFRLTEHDRK
                     RGYTLEVRVSNTSAIALYERLGFNATGIRRGYYTDNREDALIMWKDPVAKSGTVAAAR
                     APAR"
     gene            1056..2021
                     /gene="tsaD"
                     /locus_tag="VFO88_03500"
     CDS             1056..2021
                     /gene="tsaD"
                     /locus_tag="VFO88_03500"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9544617.1"
                     /translation="MILGLETSCDETAAALVTRDGEVRASVVASQAELHARYGGVVPE
                     VASRRHLELVEPVVREALGQADARLEDVEAVAVTRGPGLIGALLVGVSAAKALAWSRR
                     LPLAPVDHLHGHVAALYLRPEPLDPPFLALLATGGHTLLLDVQDRSGYRVVGRTRDDA
                     AGEAFDKGARLLGLGYPGGAALDRLAREGDPEAYRFPVARVPGLDFSFSGLKTALVYT
                     VRELEPDELERRRADLAASYQRAIVRALVERTRAAAEELGAGRVAVVGGVAANSELRA
                     SLPDATLAPLALCTDNAAMIASAARYVALVPFPDYLGLDAYATAA"
     gene            1999..>2841
                     /locus_tag="VFO88_03505"
     CDS             1999..>2841
                     /locus_tag="VFO88_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9544618.1"
                     /translation="MRMRPRRRSFPARLRVAVLALVAAGAAGVAVVPPVIGAGESAVR
                     AAASAWDGVFGERAQPVAEGRMIVVLEAPSVAERIAQAETPPTAEDQMRWVAEADASQ
                     RLLVARLRERGLDVERVRSFTKTLNGFSAVLDARAAAELGRMDGIAGVYPVRTLYPAA
                     LTAEVLAGAQFRAGAGRRPEPRLAGYDGSGIRIALLDGGVALDHPSLEGRVLRGFDLV
                     DRDRLPEAEAKPDEPAVVETHGTRMAGILVGSGGPAGLEGVAPGAQLLPIRILGWERA
                     ADGEY"
BASE COUNT          313 a         1082 c         1082 g          364 t
ORIGIN      
        1 tctccgggac ggggacgtgc tcggcgagcg gcggacgaag gccgcgcgcc tgctcgccga
       61 cgcagaggag ctctgtgcgg ccgcaggcat ccagccggcc gagctcgacc ggctcgtcgc
      121 gggcacgggg cccggcggct acaccggcct gcgcatgggg ctcgtgaccg cgcgcgtgct
      181 cgccttctcc ctcggcctcg acgccgcggg cgtctcgacg ctcgacgcgc tcgccgccgg
      241 tgcgccgggc gcggaaccgc tcctcgacgc gcgacgcggc caggtcttcg cgcgcgggat
      301 gaacggcgag ccgcgcgtcc tcgcgcccga ggacctcgag gtcgagcccg gcgccgtgtt
      361 cgtcggcgac ggggcggtgc gctaccgcga gctcgtcgag gcgcgcggcg gcgtcgtgcc
      421 cgcggacgac gacgagcgcc acgtcccgtg ggcgcgtcac cacgccgggc tcgcgcgcga
      481 cttcggcccg gccgagctcc tcgagccgct ctacctccgc gagccggacg ccgagcggag
      541 cctccgggaa ggcaggttgc gcacatgacg atcgagctcc ggcgcctcac gctcggcgac
      601 ctcggcgcga tcgagcggat cgagcgcgag gcgtacccga cgccctggtc gcgctcgatg
      661 ttcgcgggcg aggtggcgaa gccgtcctcg atctcgctcg gcgcgttcga cctcgacggg
      721 gaggagacgc tcgtcggcta cctgatcatc tcccgctacg tcgacgcctg gcacgtgatg
      781 aacatcgcgg tcgcgcacgc gtaccgccgc cgtgggatcg ccatccggat gctggaggag
      841 ctcttccgcc tcacggagca cgaccgcaag cgcgggtaca cgctcgaggt gcgcgtctcg
      901 aacaccagcg cgatcgccct ctacgagcgg cttggcttca acgcgaccgg gatccggcgc
      961 ggctactaca cggacaaccg cgaggacgcc ctgatcatgt ggaaggaccc ggtggcgaag
     1021 agcgggaccg tcgcggcggc gcgggcgccg gcccggtgat cctcgggctc gagacgtcct
     1081 gcgacgagac ggccgcggcg ctcgtcacgc gcgacgggga ggtgcgcgcg agcgtcgtcg
     1141 cctcgcaggc ggagctccac gcgcgctacg ggggcgtcgt ccccgaggtc gcctcgcggc
     1201 ggcacctgga gctcgtcgag ccggtcgtcc gcgaggcgct cgggcaggcg gacgcgcggc
     1261 tcgaggacgt cgaggcggtc gcggtcacgc gcgggcccgg gctgatcggc gcgctcctcg
     1321 tcggcgtctc ggcggcgaag gcgctcgcgt ggtcgcgccg gcttccgctc gcgccggtcg
     1381 accacctgca cggccacgtc gccgcgctgt acctccggcc ggagccgctc gacccgccgt
     1441 tcctcgcgct gctcgcgacc ggcgggcaca cgctcctcct cgacgtgcag gaccgctcgg
     1501 gctaccgcgt ggtcggccgc acccgcgacg acgcggcggg ggaggcgttc gacaagggcg
     1561 cgcgccttct cggcctcggc tacccgggcg gcgcggcgct cgaccggctc gcgcgcgagg
     1621 gcgacccgga ggcgtatcgc tttcccgtcg ctcgcgtgcc ggggctcgac ttctccttct
     1681 ccggcctgaa gacggcgctc gtctacacgg tgcgcgagct cgagccggac gagctcgaac
     1741 ggcgcagggc ggatctcgcc gcctcgtacc agcgcgcgat cgtccgcgcg ctcgtcgagc
     1801 gcacgcgggc ggccgcggag gagctcggcg ccgggcgggt cgcggtggtg ggcggcgtcg
     1861 ccgcgaactc cgagctccgg gcgtcgctcc ccgacgcgac gctcgcaccg ctcgcgctct
     1921 gcaccgacaa cgcggcgatg atcgcctcgg ccgcccggta tgtcgcgctt gtcccgtttc
     1981 ccgactatct tggtctggat gcgtatgcga ccgcggcgta ggtcgttccc cgcgcgcctg
     2041 cgcgtggccg tgctcgccct cgtcgccgcc ggcgccgcgg gcgtggccgt cgtcccgccc
     2101 gtgatcggcg cgggcgagtc ggccgtgcgc gccgccgcct ccgcctggga cggcgtcttc
     2161 ggcgagcgcg cgcagcccgt ggcggagggc cgcatgatcg tcgtcctcga ggcgccctcc
     2221 gtggcggagc ggatcgcgca ggcggagacg ccgccgaccg ccgaggacca gatgcgctgg
     2281 gtcgcggagg ccgacgcgtc gcagcggctc ctcgtcgccc gcctgcgcga gcgcgggctc
     2341 gacgtcgagc gcgtccggag cttcacgaag acgctgaacg ggttctccgc cgtgctcgac
     2401 gcgcgcgccg ccgcggagct cggccggatg gacggcatcg cgggcgtcta tccggtccgc
     2461 acgctttacc cggccgccct gaccgccgag gtcctggccg gggcgcagtt ccgcgccggc
     2521 gccggccgcc ggcccgagcc ccgcctcgca ggctacgacg ggagcgggat caggatcgcg
     2581 ctgctggacg gcggcgtcgc gctcgaccac ccctcgctcg aggggagggt cctgcgcggg
     2641 ttcgacctcg tcgaccgcga ccgcctgccc gaggccgagg cgaagcccga cgagccggcc
     2701 gtcgtcgaga cccacgggac gcgcatggcc ggcatcctcg tcggaagcgg cgggcccgcc
     2761 gggctcgagg gcgtcgcgcc gggcgcgcag ctcctgccga tcaggatcct cggctgggag
     2821 cgcgccgccg acggcgagta c
//
LOCUS       DASTFC010000118         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1419383,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000118 DASTFC010000000
VERSION     DASTFC010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419383"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFO88_03510"
     CDS             <1..255
                     /locus_tag="VFO88_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544619.1"
                     /translation="DGFVAKDGSWTCAQTHTFSAVTIGGEGAATPPPAPPAQAAPQPA
                     ADDGMSGALAVALAVAIVALGLGAAVLLGRRRQPDAAPHA"
     gene            338..538
                     /locus_tag="VFO88_03515"
     CDS             338..538
                     /locus_tag="VFO88_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544620.1"
                     /translation="MAANVIEERETEAERILSWRLEELERAGYDARAAWELAERADVD
                     LHRAVGLVRRGCPHQTALRILL"
     gene            591..1460
                     /gene="lgt"
                     /locus_tag="VFO88_03520"
     CDS             591..1460
                     /gene="lgt"
                     /locus_tag="VFO88_03520"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9544621.1"
                     /translation="MQALASIPSPSSGALEVGPLTLHAYGFMLLLGILAATAMTGIRW
                     TRRGGDWDLVLRVAMWGVVGGIVGARLYHVATSWSEVPDPKWQGVFEIWRGGLGIWGG
                     IAGGVLAGWIALRRSGQPLFPFMDAVAPGLLLAQAIGRWGNYFNQELFGEPTSRPWAL
                     EIDVENRPVEYLANETFHPLFLYESLWALLGVGVLLVVERLFRIRPPGLFCLYVLWYT
                     GMRFFIEFLRIDDAHEFFGLRLNAWVSAVLFVLAAIGLLWSQRRKEKPPKPKDRPIPR
                     PEKTMAVPKGRVR"
     gene            1481..2218
                     /locus_tag="VFO88_03525"
     CDS             1481..2218
                     /locus_tag="VFO88_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HET9544622.1"
                     /translation="MTGVAVRDLPLDLDAFQGPFDLLLTLVLRDELELSDVEVAEIVL
                     AFVARAAERETLDLEACGEFLVLVAALLELKARELFPGEEDVLDLDPEEAADELAERL
                     AAYRRVKAAAAWLDGRLGESGERWFRVGPAPVPVAAAPVELPPQNVASLAEAMRRLAV
                     EPPAPSLRHMGTFPPVSQFLARFRAVLSRSGRFLFDREVARLSRVEQAVAFLALLELR
                     KAGEVTIRQLAPFEPISVAACSSPTSA"
BASE COUNT          261 a          816 c          844 g          337 t
ORIGIN      
        1 gacggcttcg tggcgaagga cggatcgtgg acgtgcgcgc agacgcacac gttctccgcg
       61 gtcacgatcg gcggcgaagg cgccgcgacg ccgccgcccg cgccgcccgc gcaggcggcc
      121 ccgcagccgg ccgccgacga cgggatgagc ggcgctctcg ccgtcgcgct cgcggtcgcg
      181 atcgtcgcgc tcggcctcgg cgccgcggtc ctcctcggcc gccggcgcca gcccgacgcc
      241 gccccgcacg cctgagccgg cgcgcatccc cggatcgggc ggagaaatcc cccgatccgg
      301 ggagagacgc cgcgcgcacc ggggcgatgg aggggctatg gcggcgaatg tcatcgagga
      361 acgcgagacc gaggccgagc ggatcctgag ctggcggctc gaggagctcg agcgggccgg
      421 gtacgacgcc cgggccgcgt gggagctcgc cgagcgcgcc gacgtggatc tgcatcgcgc
      481 ggtcgggctc gtgcggcgcg ggtgcccgca ccagaccgcg ctccgcatcc tcctctagcg
      541 aatcccatcg gtcggacggg ggcgccggga acccttaagg ttgtcccggc atgcaggcgc
      601 tcgcctccat cccgagccca tcgagcggcg cgctcgaggt cgggccgctc acgctccacg
      661 cgtacggctt catgctcctg ctcgggatcc tcgccgcgac cgcgatgacc ggcatccgct
      721 ggacgcgccg cgggggcgac tgggatctcg tcctgcgcgt cgccatgtgg ggcgtcgtcg
      781 gcgggatcgt cggcgcgcgc ctctaccacg tcgccacgag ctggagcgag gtaccggatc
      841 cgaagtggca gggcgtgttc gagatctggc gcggcggcct cggcatctgg ggcgggatcg
      901 cgggcggcgt cctcgcaggc tggatcgcgc tgcgccgctc cgggcagccg ctcttcccgt
      961 tcatggacgc cgtcgccccg gggctcctcc tcgcgcaggc gatcgggcgc tgggggaact
     1021 acttcaacca ggagctcttc ggcgaaccga cgtcgcgtcc ctgggcgctc gagatcgacg
     1081 tcgagaaccg gccggtcgag tacctcgcga acgagacgtt ccacccgctc ttcctctacg
     1141 agtcgctctg ggcgctcctc ggcgtcggcg tcctgctcgt cgtcgaacgg ctcttccgga
     1201 tccgcccgcc gggcctcttc tgcctctacg tcctctggta cacgggcatg cggttcttca
     1261 tcgagttcct gcggatcgac gacgcgcacg agttcttcgg gctgcggctg aacgcctggg
     1321 tgtcggccgt cctcttcgtg ctcgccgcga tcgggctcct gtggtcgcag cggcgcaagg
     1381 agaagccgcc caagccgaag gaccgcccga tccccaggcc ggagaagacg atggccgttc
     1441 cgaaggggcg cgtccggtag cgggagtagg gttcggcgcc gtgaccgggg tcgccgtccg
     1501 ggatctcccg ctcgacctgg acgcctttca ggggccgttc gacctgctcc tgaccctcgt
     1561 cctgcgggac gagctcgagc tctcggacgt cgaggtggcc gagatcgtgc tcgcgttcgt
     1621 cgcgcgcgcg gcggagcgcg agacgctcga cctcgaggcg tgcggcgagt tcctcgtgct
     1681 ggtggccgcg ctgctcgagc tgaaggcgcg cgagctcttc ccgggggagg aggacgtgct
     1741 cgacctcgat ccggaggagg cggcggacga gctcgccgag cggctcgcgg cctaccggcg
     1801 cgtgaaggcg gccgccgcgt ggctggacgg gcggctcggg gagtccggcg agcggtggtt
     1861 ccgggtcggg cctgcgccgg ttccggtcgc cgcggcgccc gtggagctgc cgccgcagaa
     1921 cgtcgcctcg ctcgcggagg cgatgcgccg gctcgcggtc gagccgcccg cgccctcgct
     1981 ccggcacatg gggacgttcc cgccggtctc gcagttcctc gcccggttcc gcgccgtgct
     2041 ctcacggagc gggcggtttc tcttcgaccg cgaggtggcg cggctgtcgc gggtcgagca
     2101 ggcggtcgcg ttcctcgcgc tcctggagct ccggaaggcc ggggaggtga cgatccgcca
     2161 gctcgcgccg ttcgagccga tctcggtagc cgcatgcagc agcccaacct ccgcgtagtc
     2221 gagaaccccg tcgaccggct ggcccgcgtg ctcgaggg
//
LOCUS       DASTFC010000119         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_661191,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000119 DASTFC010000000
VERSION     DASTFC010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661191"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VFO88_03530"
     CDS             complement(<1..1310)
                     /locus_tag="VFO88_03530"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HET9544623.1"
                     /translation="MTTSDRYDQVHGNVGAGQPERRSVLGRSPIFTPEVLNDIHMKAE
                     LGRYRMRGFSMFKKIPHWDDLMFMPGTLTRFVIEGYREKCETKTVLGSRFAKKPIELD
                     IPIYITGMSFGALSLEAKMALAKGASMAGTATCSGEGGMIPPERDLSTKWYYQLIQSR
                     YGFNPHHLMLADACEFFIGQGCKVGLGGHLMGQKVTEQVAEMRSLPPGIDQRSPARHP
                     DWLGPDDLSLKVQEIREATDYQIPIQLKLGAARVYDDVRMAAKCGPDVIYLDGAEGGT
                     GAGPHVATEETGIPLMAAIPEARRALEDVGLADEIQLVVAGGIRNGADVAKCLALGAD
                     AIAIGHAALVALNCNKEIPGVTDYEGTMGVPAGECYHCHTGRCPVGITTQDPELRKRL
                     IVDEAAERVYNFLHTMTLELQMLARACGKTNVHSLEPEDLCALTV"
     gene            complement(1307..2095)
                     /locus_tag="VFO88_03535"
     CDS             complement(1307..2095)
                     /locus_tag="VFO88_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544624.1"
                     /translation="MAAVGEQRITYDARGLAEPNAEAPRIATVEDGRATFDAHDLTTR
                     QINLELRRLLYEEGITDVTVLNPGAKHSLGVGILTRCRLRFEGSLGYFGLGLIDGPEI
                     VVTGRVGWSACENMMSGVVVVESNAGSLTGAAMRGGDLVVKGRVGARTGIDQKGGTII
                     VGEDAGSMTGFMMQRGRMIILGNAGPGLGDSMYDGTIYVGGTVKSLGIDCVPGEWTDA
                     DTELIERKFSIYGLGDPPDLQKFVCGKKLYNYDNLEPAERKLVL"
     gene            complement(2083..3030)
                     /locus_tag="VFO88_03540"
     CDS             complement(2083..3030)
                     /locus_tag="VFO88_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544625.1"
                     /translation="MCGIAGLIYRDGTHEIGRDLTRMLQSMKHRGPDSTGYALYGPRG
                     HRILMRYTLADANTPRDFEFGDRLRRHQAQVLDRLRGLGATVHDVEAETEYAYRIELE
                     LPAANGGEPDLKAIADRLEDIPDCEVLSLGHSLEIVKDLGDAETVSDQYELKGFTGTH
                     AIGHVRMATESDVDISGAHPYWAYPFSDVAVVHNGQLTNYFQWKRRLERSGHRFQSEC
                     DSEIIAVYLAEKMSGGASLEDAMRDSLEELDGVFTYIAVTDDALGVAKDEMAAKPLVL
                     YESDDVVALASEEIAIRAVLDREIETYDPYESEVLVWQR"
     gene            complement(3051..4529)
                     /locus_tag="VFO88_03545"
     CDS             complement(3051..4529)
                     /locus_tag="VFO88_03545"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET9544626.1"
                     /translation="MAVVERPSLVQEVLSKGPIPDPHPTLDEARNDPARWAKIDEIRA
                     RIDELGIKYIFFQQVSVSGHVNGKGVAASMWERVASEGYQLVYGATADLFVDRADRYI
                     GFGPEESELAAIADLDTFQQLPWDSRVARVFCDCYDTETGKLLEADPRQNLKRTVNEI
                     EAELGYTFLCGIEPEMMWMRRPTGADAGAAEGLTKPWCYHINQFEELRPVILDVIEYG
                     EKLGIHPTYGDHEDSPGQLELNFLFDRAVRTADNLSTYRQLCAAVAREHTRRGTYGDD
                     YEVLATFMPKPFTGVSANGAHHHFTLVDEEGNNVFYDKDGPAKLSKIGLQFMGGILEH
                     ARGLCAITAPTVNSYKRFWDFGFWAPIYKDYGWQNRTTLVRVASGGRFEFRAVDSSCN
                     PYLTQAALLRAGLDGVKRKLDPGKPQQRNIYDVIEEGAEVERVPDHLGEALQALEEDD
                     VVKSALPGRLLDVYLHYKKDEWERFLAAVTDWERDRYLDVLP"
     gene            complement(4830..5987)
                     /locus_tag="VFO88_03550"
     CDS             complement(4830..5987)
                     /locus_tag="VFO88_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9544627.1"
                     /translation="MEAVLTEESRGFVARTGLPELDEPLGGLYRGDNVVWEVSAGASA
                     TPFLRAVTRDASAYDFAAYVALASHPDEIAAEHPRLRIVHAHAGSALGSPGPLLDELA
                     RRCAAPARNLVVFDPLDTMVARWGSEATRRFFGLCCPRLLELGVIAHWALDADADQAL
                     RRHVDEVTQCVVAISRDRVRIAKAQGRPPGAEGAVFRYRLEDGLPLLEPAPVSGRLGA
                     GLRAARQARGLSQADLARLAGVSPSAISQAERGQRGLSLETVLELSSRLGVSLDELLR
                     GDRSPGFRVGRLREEGRESRGQPLALLDDPGPGLRAYVVSLAPGASGRPHLGHKHVEL
                     VAVARGLVQVVTSGGPAVVRGGEVVLADEALVEGWRNMSDGDALLFWIVRD"
BASE COUNT          856 a         2209 c         2158 g          964 t
ORIGIN      
        1 accgtcagag cgcagaggtc ctcgggctcg agcgagtgga cgttcgtctt cccgcacgcg
       61 cgcgcgagca tctggagctc gagcgtcatc gtgtggagga agttgtagac ccgctcggcc
      121 gcctcgtcga cgatcaggcg cttgcggagc tccgggtcct gcgtcgtgat cccgacgggg
      181 cagcggcccg tgtggcagtg gtagcactcg ccggccggga cgcccatcgt gccctcgtag
      241 tcggtgacgc cggggatctc cttgttgcag ttgagcgcca cgagcgccgc gtggccgatc
      301 gcgatcgcgt ccgcgccgag cgcgaggcac ttcgcgacgt ccgcgccgtt ccggatcccg
      361 cccgccacga cgagctggat ctcgtccgcg agcccgacgt cctcgagcgc gcgccgcgcc
      421 tcggggatcg ccgccatgag cgggatcccg gtttcctcgg tcgccacgtg gggcccggcg
      481 ccggtgccgc cctccgcgcc gtcgagatag atgacgtccg gcccgcactt cgccgccatc
      541 cgcacgtcgt cgtagacgcg tgccgcgccg agcttgagct ggatcgggat ctggtagtcg
      601 gtcgcctcgc ggatctcctg caccttgagc gagaggtcgt cgggcccgag ccagtccggg
      661 tgccgcgccg gcgagcgctg gtcgatcccg ggcgggagcg agcgcatctc cgccacctgc
      721 tcggtcacct tctggcccat caggtggccg ccgagcccga ccttgcagcc ctgcccgatg
      781 aagaactcgc acgcgtccgc gagcatcagg tggtgcgggt tgaacccgta ccggctctgg
      841 atgagctggt agtaccactt ggtcgagagg tcgcgctccg gcgggatcat cccgccctcg
      901 cccgagcacg tcgccgtgcc ggccatggac gcgcccttcg cgagcgccat cttcgcctcg
      961 agcgagagcg cgccgaagct catgccggtg atgtagatcg ggatgtcgag ctcgatcggc
     1021 ttcttcgcga accgcgagcc gagcaccgtc ttcgtctcgc acttctcgcg gtagccctcg
     1081 atcacgaacc gggtcagcgt gcccggcatg aacatgaggt cgtcccagtg cgggatcttc
     1141 ttgaacatgg agaagccccg catccggtag cggccgagct cggccttcat gtggatgtcg
     1201 ttcaggacct ccggcgtgaa gatcgggctc cggccgagca ccgagcgccg ctccggctgc
     1261 ccggcgccga cgtttccgtg cacctggtcg taacggtccg aggtcgtcat aggacgagct
     1321 tcctctccgc aggctcgagg ttgtcgtagt tgtagagctt cttgccgcag acgaacttct
     1381 ggaggtccgg cgggtcgccg agcccgtaga tcgagaactt ccgctcgatg agctcggtgt
     1441 ccgcgtccgt ccactcgccg gggacgcagt cgatcccgag cgacttgacc gtgccgccga
     1501 cgtagatcgt gccgtcgtac atcgagtcgc cgagcccggg ccccgcgttc ccgaggatga
     1561 tcatgcggcc gcgctgcatc atgaagcccg tcatcgagcc ggcgtcctcg ccgacgatga
     1621 tcgtcccgcc cttctggtcg atccccgtgc gggcgccgac gcggcccttg acgacgaggt
     1681 cgccgccgcg catcgcggcg cccgtgagcg agccggcgtt cgactcgacc acgacgacgc
     1741 cggacatcat gttctcgcag gccgaccagc cgacccggcc ggtcacgacg atctccggcc
     1801 cgtcgatcag gccgaggccg aagtagccga gcgagccttc gaaccgcagg cggcagcgcg
     1861 tgaggatccc gacccccagt gagtgcttcg cgcccgggtt caggacggtg acgtccgtga
     1921 tcccctcctc gtagagcagc cggcgcagct cgaggttgat ctgccgggtg gtcaggtcgt
     1981 gcgcgtcgaa cgtcgcgcgc ccgtcctcga cggtcgcgat gcggggcgcc tcggcgttcg
     2041 gctcggcgag cccgcgcgcg tcgtacgtga tccgctgctc gcctaccgct gccatacgag
     2101 cacctcgctc tcgtacgggt cgtaggtctc gatctcgcgg tcgaggacgg cacggatcgc
     2161 gatctcctcg gaggcgagcg cgacgacgtc gtccgactcg tagaggacga gcggcttcgc
     2221 ggccatctcg tccttcgcca cgccgagcgc gtcgtcggtc acggcgatgt acgtgaagac
     2281 gccgtcgagc tcctcgagcg agtcgcgcat cgcgtcctcg agcgacgcgc ccccgctcat
     2341 cttctccgcg aggtagacgg cgatgatctc cgagtcgcac tcggactgga accggtgccc
     2401 gctccgctcg agccggcgct tccactggaa gtagttcgtg agctggccgt tgtgcacgac
     2461 cgcgacgtcc gagaacggat acgcccagta cgggtgcgcg cccgagatgt cgacgtccga
     2521 ctcggtcgcc atccggacgt ggccgatcgc gtgcgtcccg gtgaacccct tcagctcgta
     2581 ctggtcggag acggtctccg cgtcgccgag gtccttcacg atctcgagcg agtggccgag
     2641 cgagaggacc tcgcagtcgg ggatgtcctc gagccggtcg gcgatcgcct tgaggtcggg
     2701 ctcgccgccg ttcgcggccg gcagctcgag ctcgatccgg tacgcgtact ccgtctcggc
     2761 ctcgacgtcg tgcacggtcg cgccgaggcc gcgcagccgg tcgagcacct gcgcctggtg
     2821 ccgcctcagc cggtcgccga actcgaagtc ccgcggcgtg ttcgcgtccg cgagcgtgta
     2881 gcgcatcagg atccggtgcc cgcgcgggcc gtagagcgcg tagcccgtcg agtcggggcc
     2941 gcggtgcttc atggactgga gcatccgcgt caggtcgcgc ccgatctcgt gggtgccgtc
     3001 gcggtagatc agccctgcga ttccgcacat ggtcgtttac ccctttgtcg ctacggcagg
     3061 acgtcgaggt agcggtcgcg ctcccagtcc gtgacggccg cgaggaaccg ctcccactcg
     3121 tccttcttgt agtgaagata gacgtcgagg agccggccgg gcagggcgct cttcaccacg
     3181 tcgtcctcct cgagcgcctg gagcgcctcg ccgaggtggt cgggcacgcg ctcgacctcc
     3241 gccccctcct cgatcacgtc gtagatgttc cgctgctgcg gcttgcccgg gtcgagcttg
     3301 cgcttgacgc cgtcgaggcc ggcgcgaagg agcgccgcct gggtcaggta cgggttgcag
     3361 gaggagtcga cggcgcggaa ctcgaagcgc ccgccgctcg ccacgcgcac gagggtcgtg
     3421 cggttctgcc agccgtagtc cttgtagatc ggcgcccaga acccgaagtc ccagaagcgc
     3481 ttgtacgagt tcacggtcgg ggccgtgatc gcgcagagcc cacgcgcgtg ctcgaggatg
     3541 ccgcccatga actgcaggcc gatcttggag agcttcgcgg ggccgtcctt gtcgtagaag
     3601 acgttgttgc cctcctcgtc cacgagcgtg aagtggtggt gcgccccgtt cgccgacacg
     3661 ccggtgaacg gcttcggcat gaaggtcgcg agcacctcgt agtcgtcgcc gtacgttccg
     3721 cggcgggtgt gctcgcgcgc gaccgccgcg cagagctggc ggtacgtcga gaggttgtcc
     3781 gccgtccgca ccgcgcggtc gaagaggaag ttcagctcga gctggccggg cgagtcctcg
     3841 tggtcgccgt acgtcgggtg gatcccgagc ttctcgccgt actcgatcac gtcgaggatg
     3901 accgggcgga gctcctcgaa ctggttgatg tggtaacacc acggcttcgt caagccctcc
     3961 gcggcgccgg cgtcggcgcc cgtcggccgc cgcatccaca tcatctcggg ctcgatcccg
     4021 cagaggaagg tgtagccgag ctcggcctcg atctcgttga ccgtgcgctt gaggttctgg
     4081 cgcgggtcgg cctcgaggag cttgcccgtc tcggtgtcgt agcagtcgca gaacacgcgc
     4141 gcgacgcgcg agtcccaggg gagctgctgg aacgtgtcca ggtcggcgat cgcggcgagc
     4201 tcggactcct ccgggccgaa gccgatgtag cggtcggcgc ggtcgacgaa gaggtcggcg
     4261 gtcgcgccgt agacgagctg gtacccctcc gacgcgacgc gctcccacat ggaagccgcg
     4321 acgcccttgc cgttcacgtg gcccgagacc gagacctgct ggaagaagat gtacttgatc
     4381 ccgagctcgt cgatccgcgc gcggatctcg tcgatctttg cccagcgcgc aggatcgttc
     4441 cgcgcttcgt cgagcgtcgg atgggggtcg gggatcggcc ccttggacag cacctcctgg
     4501 acgagcgacg gccgctctac gacagccacg tagacctccc ttcctgggtt ggacgccacc
     4561 ttatcgccgc gccgaggccc gaccaaggcg cctccggccg gcccgcgggc attgtggaac
     4621 ttccgctcac gtcccccgcc cgccgatgga ggaagtggcc attcggaact cgtttcaaaa
     4681 cgaagccttg agccctcggg ccgcgctgga ctgcccctgc ccgcgtcctc ggtctcgccc
     4741 gtagcctccg gctacgaacg agacgctgcg tccttggcag gagccgccct tcgcaccccg
     4801 acgacgaagc gtcatttcga aacgagttct cagtcacgca cgatccagaa gaggagcgcg
     4861 tcgccgtcgc tcatgttccg ccagccctcg acgagcgcct cgtcggcgag gacgacctcc
     4921 cctccccgca cgacggccgg gccgccggac gtgacgacct gcacgagccc gcgcgcgacg
     4981 gcgacgagct cgacgtgctt gtgcccgagg tgcggccgcc cggacgcgcc gggcgcgagc
     5041 gagacgacgt acgcgcggag cccgggcccc gggtcgtcga ggagcgcgag cggctgcccg
     5101 cggctctcgc ggccctcctc gcgcaggcgc cccacgcgga agccgggcga ccggtcgccg
     5161 cggaggagct cgtcgagcga gacgccgagc cggctcgaaa gctcgagcac ggtctcgagc
     5221 gagagcccgc gctgcccgcg ctccgcctgc gagatcgcgc tcggcgagac gcccgcgagc
     5281 cgcgcgaggt cggcctggct cagcccgcgc gcctgccgtg ccgcgcgcag ccccgctccg
     5341 agccggcccg acacgggcgc cggctcgagc agcggcagcc cgtcctccag ccggtagcgg
     5401 aacacggccc cctcggcgcc gggcgggcgg ccctgcgcct tggcgatcct gacgcggtcg
     5461 cgcgagatcg cgacgacgca ctgcgtcacc tcgtccacgt ggcgccggag cgcctggtct
     5521 gcgtccgcgt cgagcgccca gtgcgcgatc accccgagct cgagcaggcg cgggcagcag
     5581 agcccgaaga accgccgcgt cgcctcggag ccccagcggg cgaccatcgt gtcgagcggg
     5641 tcgaagacga cgaggttgcg ggccggcgcg gcgcagcggc gggcgagctc gtcgaggagc
     5701 ggcccggggc tgccgagcgc cgaccccgcg tgcgcgtgga cgatgcgcag gcgcgggtgc
     5761 tcggccgcga tctcgtccgg atgcgacgcg agcgcgacgt aggcggcgaa gtcgtacgcc
     5821 gacgcgtccc gagtgaccgc ccggaggaac ggcgtcgcgg acgcgcccgc gctcacctcc
     5881 cagacgacgt tgtccccgcg gtagagcccg ccgagcggct cgtccagctc gggcaggccc
     5941 gtgcgggcga cgaagccccg ggactcctcc gtaagcaccg cttccatctt ctcacggcgt
     6001 gcgaaccgct gctatccgtc gtgtggagag cacacacctg gaggcccaaa ggatgggtga
     6061 gcgggacgtg ggccgcatcc ggaggccgcc gtaggctcgc ccggtcgcat ccgccccacg
     6121 aactagcggc gtcacgagcg gagcggggct cgatgcggca agcggctagg accccgtcga
     6181 aggaggc
//
LOCUS       DASTFC010000120         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1089998,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000120 DASTFC010000000
VERSION     DASTFC010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089998"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..451
                     /locus_tag="VFO88_03555"
     CDS             38..451
                     /locus_tag="VFO88_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544628.1"
                     /translation="MVRAAGFGGMVAAMESYDHLRDEELSGESAAVRDPGLDRHEWET
                     AWEGLQEQLHDAPAEALPELGDLIAEMLEARGYAVEDPVADDGIEPDVLVSFRSAREI
                     TVMVDRGDDVDPSDLGEAIHAYREIYEQLIDRPDN"
     gene            complement(448..582)
                     /locus_tag="VFO88_03560"
     CDS             complement(448..582)
                     /locus_tag="VFO88_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544629.1"
                     /translation="MTHHDEHDERDEKLLEAQEGTGYGEDEGEREEALEPDDAASDDD
                     "
     gene            complement(612..1049)
                     /locus_tag="VFO88_03565"
     CDS             complement(612..1049)
                     /locus_tag="VFO88_03565"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9544630.1"
                     /translation="MIDVVVEQEVARPREEVAAYATDWRNDPEWIGAVNASRLVGEGP
                     FGVGARVARQASFLGRRFEYVNEVTELEPGTRLGMRSVAGPFPMRVVYEWEDADGGSL
                     MRIRASGDASAFYRLAAPLLGRAVRRGIAGDLARLRAAVESRG"
     gene            complement(1046..1504)
                     /locus_tag="VFO88_03570"
     CDS             complement(1046..1504)
                     /locus_tag="VFO88_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9544631.1"
                     /translation="MSAVLRLSRRFAAPPERVFAAWTHPDVLREWWAANVEWEGAEAE
                     VDLRPGGRYRLGMRDTASGAVHVVTGEYVEVDPPSRLAYTWTWEGEPTEMRGSEDTHV
                     VVEFVPDGGGTLVLLTHSGFADENIRDLHDAGWTTCLDNLERRVLGRQPA"
     gene            complement(1501..1833)
                     /locus_tag="VFO88_03575"
     CDS             complement(1501..1833)
                     /locus_tag="VFO88_03575"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9544632.1"
                     /translation="MNSEPTLDRAFLALAHPARRAIVARLTAGSAPVAEAARGVRASK
                     PAISKHVKVLEEAGLVRRTVEGRSHRLRLEPAALDRTSGWLDRQRALWEAKLDAVERH
                     LAREDERA"
     gene            complement(1859..2350)
                     /locus_tag="VFO88_03580"
     CDS             complement(1859..2350)
                     /locus_tag="VFO88_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9544633.1"
                     /translation="MTEPIDPGARVGHVHLKVADLERALGFWRDVLGFEERARYGDQA
                     VFISAGGYHHHIGLNTWESKGGSPPPPGTTGLYHVAILYPTREALAKALRRVLEAGIR
                     LDGASDHGVSEALYLRDPDGNGVELYCDRPREEWPRAENGDGVGMFTAPLDVRALLEL
                     ASD"
     gene            complement(2507..3163)
                     /locus_tag="VFO88_03585"
     CDS             complement(2507..3163)
                     /locus_tag="VFO88_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544634.1"
                     /translation="MSKRTLARVGGLAAILAGVFWGFTLPLIATQATDDPVGLRYDDF
                     NRWLTLPLALLLVTLVVLRALLGRRLPAQGRLGTTLAAVGAGLMLLGNLVEFWAVMMT
                     DDYVAAIAEDRRVDEWVGSTIGWIGFLVGVFLLLVGGSLVALAAQRARALPAWVGIIL
                     AATAPALLAAAVLWASSVEATIVVAVALGLAWNALGLWMRARATGLAEAAFVATEPRA
                     "
BASE COUNT          455 a         1259 c         1189 g          423 t
ORIGIN      
        1 aggacgttca tcgcgggcga gtctaaagcc ccggtcggtg gttcgcgccg cggggttcgg
       61 gggaatggtg gccgcgatgg agagctacga ccacttgcgc gacgaggagc tttcgggtga
      121 aagtgcagcc gtgcgcgacc ccgggctcga tcgtcacgaa tgggagaccg cctgggaggg
      181 gctccaggag cagctccacg acgcgcccgc cgaggcgctc cccgagctcg gcgacctgat
      241 cgccgagatg ctcgaggccc gcggctatgc ggtcgaggac ccggtggccg acgacgggat
      301 cgagccggac gtgctcgtct cgttccgctc cgcccgcgag atcacggtga tggtcgaccg
      361 cggcgacgac gtcgacccct cggacctcgg cgaggcgatc cacgcgtacc gcgagatcta
      421 cgagcagctc atcgaccgcc cggacaacta gtcgtcgtcg gaggccgcgt cgtcgggctc
      481 gagcgcttcc tcgcgctcgc cctcgtcctc gccgtagccc gtgccctcct gcgcctcgag
      541 gagcttctcg tcgcgctcgt cgtgctcgtc gtgatgcgtc atggcgggat gctgccccgg
      601 gggcgcgcgg ctcaaccgcg cgactcgacc gccgcccgga gccgcgcgag gtcgcccgcg
      661 atcccccggc gcaccgcgcg accgaggagc ggcgcggcga gccggtagaa cgccgacgcg
      721 tcgccggacg cgcggatccg catgagcgac ccgccgtccg cgtcctccca ctcgtagacg
      781 acgcgcatcg ggaacggccc ggcgaccgag cgcatgccga gccgcgtgcc cggctcgagc
      841 tccgtcacct cgttcacgta ctcgaacctc cgcccgagga acgacgcctg ccgggcgacg
      901 cgcgcgccga cgccgaacgg cccctcgccg acgagccggg acgcgttcac ggcgccgatc
      961 cactcggggt cgttgcgcca gtccgtggcg tacgccgcca cctcctcgcg cgggcgcgcg
     1021 acctcctgct cgacgacgac gtcgatcacg cgggctgtct cccgaggacg cgccgctcga
     1081 ggttgtcgag gcaggtcgtc cagccggcgt cgtgcaggtc gcggatgttc tcgtcggcga
     1141 agccgctgtg cgtgaggagt acgagcgtgc cgccgccgtc cgggacgaac tcgaccacga
     1201 cgtgcgtgtc ctcgctgccc cgcatctcgg tcggctcgcc ctcccacgtc cacgtgtacg
     1261 cgagccgcga aggcgggtcg acctcgacgt actcgccggt cacgacgtgc acggcgccgc
     1321 tcgccgtgtc gcgcatcccg agcctgtagc ggccgcccgg gcgcaggtcg acctcggcct
     1381 cggcgccctc ccactcgacg ttcgccgccc accattcgcg gaggacgtcc gggtgcgtcc
     1441 acgcggcgaa gacgcgctcg ggcggcgccg cgaagcgccg gctgaggcgg aggacggcgc
     1501 tcacgcgcgc tcgtcctccc gcgccagatg ccgctcgacc gcgtcgagct tcgcctccca
     1561 gagcgcacgc tggcggtcga gccagccgga cgtccggtcg agcgccgccg gctccaggcg
     1621 gaggcgatgg ctgcgtccct cgacggtccg ccgcacgagc cccgcctcct cgagcacctt
     1681 cacgtgcttc gagatcgccg gcttcgacgc ccggacgccg cgcgccgcct cggcgaccgg
     1741 cgcggatccg gcggtgagcc gggcgacgat cgcgcgccgc gcggggtgcg cgagcgcgag
     1801 gaacgcccgg tcgagtgtcg gctcactgtt caccgatcgg tgaactatag cgccggtgtc
     1861 agtccgaggc cagctcgagg agcgcgcgga cgtcgagcgg cgccgtgaac attccgacgc
     1921 cgtcgccgtt ctcggcccgc ggccattcct cccgcggtcg gtcgcagtag agctccacgc
     1981 cgttcccgtc ggggtcgcgc agatagagcg cctcgctgac gccgtggtcg gacgcgccgt
     2041 cgagacggat ccccgcctcg agcacgcgcc gcagcgcctt cgcgagcgcc tcgcgtgtcg
     2101 gatagaggat tgccacgtgg tagagcccgg tcgtcccggg cgggggcggc gagccgccct
     2161 tgctctccca cgtgttgagg ccgatgtggt ggtggtagcc gccggcggag atgaagaccg
     2221 cctggtcgcc gtagcgcgcc cgctcctcga agccgaggac gtcgcgccag aagccgagcg
     2281 cgcgctcgag gtcggccacc ttgagatgga cgtgcccgac gcgggcgccg ggatcgatcg
     2341 gctccgtcat gccgaggact gtagcgccgt ccctttggga cgtgccggct cgcgacggcc
     2401 cgggaccatt ccccgccccc aggggtgggg tcgaggggac cgcccgcctc ccggcttcga
     2461 ggctagcggg agaaagcgcg cgagatcgtg ccgaaagcgt gccgattcac gcgcgcggct
     2521 cggtcgcgac gaacgcggcc tccgcgaggc cggtcgcccg cgcgcgcatc cacaggccga
     2581 gcgcgttcca cgcgagcccg agcgccaccg cgacgacgat cgtcgcctcc acgctcgacg
     2641 cccagagcac ggccgctgcg aggagcgccg gcgcggtcgc cgcgagaatg atcccgaccc
     2701 aggccggaag cgcgcgggcc cgctgcgcgg cgagcgcgac gagcgacccg ccgaccagga
     2761 gcaggaatac gccgacgagg aatccgatcc acccgatcgt cgacccgacc cactcgtcga
     2821 cccgccgatc ctccgcgatc gcggcgacgt agtcgtccgt catcatcacg gcccagaact
     2881 cgacgaggtt cccgagcagc atgagcccgg ccccgactgc cgcgagcgtc gtcccgagcc
     2941 gcccctgcgc gggcagccgt cgtccgagca gcgcgcgcag cacgacgagc gtcacgagca
     3001 ggagcgcgag cggcagcgtg agccaccggt tgaagtcgtc gtagcggagg ccgaccgggt
     3061 cgtccgtcgc ctgcgtcgcg atgagcggga gcgtgaaccc ccagaagact ccggccagga
     3121 tcgcggcgag gccgccgacc cgcgcgagcg tccgcttcga cacacgcgga gcgtaacgga
     3181 gcctcagggc ccgtcgagct cgcgcgcgac ggtgtccatc cgggcgaagc gcgcccccgc
     3241 ctcgccgcag tggcggatga accggtcgag catcgcgatc cggctcccgc ggccgatcac
     3301 ctgcgggtgc atcgtcgccg tcagca
//
LOCUS       DASTFC010000121         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_282279,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000121 DASTFC010000000
VERSION     DASTFC010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282279"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFO88_03590"
     CDS             complement(<1..754)
                     /locus_tag="VFO88_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9544635.1"
                     /translation="MNASRRLPWLALLLAGLVLALGAAACGGDDDDDGNGAGDTSAET
                     DEGGEEQQFRVGLVTDIGGLNDRGFNQLAYQGLQQAESELGVEIRVLESKSDADYIPN
                     LQTLADEGFNLIISVGFLMQEATATAAETYPDTNFAIIDAAFDPALPNAQGVLFKEQE
                     AGYLVGYLAGLVTESGTVSSVGGQKIPPVDKFIAGFQKGAADSNPEVQTLNAYSQDFV
                     DQAKCKEVALDQISRGSDVVFQVAGGCGLGALD"
     gene            complement(862..>2303)
                     /locus_tag="VFO88_03595"
     CDS             complement(862..>2303)
                     /locus_tag="VFO88_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9544636.1"
                     /translation="YRLPDPSLLRKSRPSDGQPAKATERTAEALVTALASHGVEATVI
                     GQVSGPRVTRYELQLAPGTKVGKVSNLKDDLAYALATTEIRILAPIPGKQAVGVEVPN
                     LAPNIVTLGDIFDTMPANASPVSVWLGKDISGASVWADLARMPHLLIAGTTGSGKSGC
                     LNTMLVSVLLRATPDEVRMILVDPKRVELGLYESIPHLLTPVVSSPKAAAAVLANVLT
                     EMERRYERMSIARARNLSELNRVLRERGEAPLPYLMVVIDELADLMMVSPQEVEDAVI
                     RLAQKSRAVGIHLVLATQRPSVDVITGMIKANVPSRIAFAVSSQTDSRVILDTSGAES
                     LLGQGDMLFKPLGTSRLQRVQGAYVSDEEIALVVEACRGQREQELDETLLEAPGVQVT
                     EGDEEGDFDPDEDPLLERAIEIVVQTQTASVSLLQRRLRVGYTRAGRLIDMLERRGII
                     SGYEGSKPRRVLVGEAELEHALAGHPSEA"
BASE COUNT          322 a          811 c          799 g          371 t
ORIGIN      
        1 cgtcgagcgc gccgagcccg cagccgcccg cgacctggaa gaccacgtcg ctgccgcggc
       61 tgatctggtc gagcgcgacc tccttgcact tcgcctggtc gacgaagtcc tgggagtagg
      121 cgttcagcgt ctgcacctcc gggttcgagt ccgcggcgcc cttctggaag cccgcgatga
      181 acttgtccac cggcgggatc ttctggccgc ccacgctcga caccgtgccg ctctcggtca
      241 cgagccccgc gaggtagccg acgaggtaac cggcctcctg ctccttgaag agcacgccct
      301 gcgcgttcgg gagcgccggg tcgaaggccg cgtcgatgat cgcgaagttg gtgtccggat
      361 aggtctccgc agccgtcgcg gtcgcctcct gcatgaggaa cccgaccgag atgatcaggt
      421 tgaagccctc gtccgcgagc gtctggagat tggggatgta gtccgcgtcc gacttggact
      481 cgagcacgcg gatctccacg ccgagctcgc tctcggcctg ctgcaggccc tggtacgcga
      541 gctggttgaa cccgcggtcg ttcagtccac cgatgtcggt cacgaggccg acccggaact
      601 gctgctcctc accgccctcg tcggtctcgg cgctcgtgtc tccggcaccg ttcccgtcgt
      661 cgtcgtcgtc gccgccgcac gccgccgcgc ccagagcgag cacgaggccc gcgagcagaa
      721 gagcaagcca gggcagcctt ctcgaagcgt tcaccgaacc tcccctctca tccacacctt
      781 ggtcatcgaa ttggccgcga tgctactcca ccccggccct ccattactag gacgcccgac
      841 cgatcggacg gttaggttcg ctcaggcttc ggacggatga ccggccagcg cgtgctcgag
      901 ctcggcctcg ccgacgagca cgcggcgggg cttcgagccc tcgtagccgg agatgatccc
      961 gcgccgctcg agcatgtcga tcagccgtcc ggcgcgcgtg tagccgacgc gcagccgccg
     1021 ctggaggagc gagacggagg cggtctgcgt ctgcacgacg atctcgatcg cgcgctcgag
     1081 gagcgggtcc tcgtccgggt cgaagtcgcc ctcctcgtcc ccctcggtca cctggacgcc
     1141 gggcgcctcg aggagcgtct cgtcgagctc ctgctcccgc tgcccgcggc aggcctcgac
     1201 gacgagcgcg atctcctcgt ccgagacgta cgcgccctgg acgcgctgga gccgcgacgt
     1261 gccgagcggc ttgaagagca tgtcgccctg cccgaggagg ctctccgcgc cggacgtgtc
     1321 gaggatcacg cggctgtccg tctgcgacga gacggcgaac gcgatccggc tcgggacgtt
     1381 cgccttgatc atcccggtga tgacgtcgac cgacggccgc tgtgttgcga gcacgagatg
     1441 gatcccgacc gcgcggctct tctgcgcgag ccggatgacc gcgtcctcga cctcctgcgg
     1501 cgagaccatc atgaggtcgg cgagctcgtc gatcacgacc atgaggtacg ggaggggcgc
     1561 ctcgccgcgc tcgcggagga ccctgttcag ctcggagagg ttgcgcgcgc gggcgatcga
     1621 catccgctcg tagcggcgct ccatctccgt gaggacgttc gcgaggacgg ccgcggccgc
     1681 cttggggctc gagacgacgg gcgtgagcag gtgcgggatc gactcgtaga gcccgagctc
     1741 gacgcgcttc gggtcgacga ggatcatcct cacctcgtcc ggcgtcgcgc gcaggaggac
     1801 ggagacgagc atcgtgttca ggcagccgct cttccccgag ccggtcgtgc cggcgatcag
     1861 gaggtgcggc atgcgcgcga ggtcggccca cacggacgcg cccgaaatgt ccttccccag
     1921 ccagacgctg accgggctcg cgttcgcggg catcgtgtcg aagatgtcgc cgagcgtgac
     1981 gatgttcggc gcgaggttgg gcacctcgac gccgaccgcc tgcttgcccg ggatcggcgc
     2041 gaggatgcgg atctccgtcg tggcgagcgc gtacgcgagg tcgtccttca ggttcgagac
     2101 cttgccgacc ttcgtcccgg gcgcgagttg gagctcgtag cgcgtgacgc ggggccccga
     2161 gacctggccg atcaccgtcg cctcgacgcc gtggctcgcg agcgcggtca cgagcgcctc
     2221 cgcggtgcgc tcggtcgcct tcgcgggctg accgtcggac gggcgagact tgcggaggag
     2281 cgacgggtcc ggcaggcggt agt
//
LOCUS       DASTFC010000122         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_529360,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000122 DASTFC010000000
VERSION     DASTFC010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529360"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFO88_03600"
     CDS             complement(<1..245)
                     /locus_tag="VFO88_03600"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544637.1"
                     /translation="MLVGARAFHAALDELPERAPATEKIRLALRAHLRLVADQLDVAT
                     VFVREWRSLDAERRDEILAERRRYEERIRALFREGREG"
     gene            447..1352
                     /gene="paaA"
                     /locus_tag="VFO88_03605"
     CDS             447..1352
                     /gene="paaA"
                     /locus_tag="VFO88_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HET9544638.1"
                     /translation="MTPRTEEAFIEHVQSGGQVEATDWMPDEYRAKLVKFIEMHGNSE
                     LMGVLPEREWILRAPTLQRKLALTAKVQDEAGHAQLIYRVVEDLGKPRDAILEDLISG
                     KSKFHNVFHYPTKTWGDVGVIAWLVDAAAIISQKALLKCSYAPYARIMKKICWEESFH
                     ILHGRDVILAMVTGTPEQRELVQEALDRWWKPLMMFHGNQIPAEKDPMYHWRIKSQGN
                     EEARQQFLDGYVPQIRELGLEIPDPKLRRDEETGRWEYTEPDWDELRHVVTGHGPMTA
                     ERLGFRRASRDETAWVRDVVLAEAA"
     gene            1357..1644
                     /locus_tag="VFO88_03610"
     CDS             1357..1644
                     /locus_tag="VFO88_03610"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544639.1"
                     /translation="MAVWEVFRQEREGQPFQHGGSLEAPNREFADAYAREFYGRRDES
                     FALWIVPREAVLEVHEPYVAPVFDRDYRRVDGYSIKVKLKEARELAGTAES"
     gene            1616..2302
                     /gene="paaC"
                     /locus_tag="VFO88_03615"
     CDS             1616..2302
                     /gene="paaC"
                     /locus_tag="VFO88_03615"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HET9544640.1"
                     /translation="MSSQAPPRADELLALADDELILGWRDSEWTGIAPFLEEDVAFSS
                     IAQTEIGHARALYELAARELGGTADTLAFDRRPDEYRSSPLVELRLVHDWGRTIARHI
                     LYELADAVRVAELKESDDADLAGLAEKIEREEAYHVMHAQMWADRLAESEEGRARLQA
                     GLDELWPYALGLVEGGQRERFLADVAERLPFFSPPEVEPVARGEHVDEWAELWEEMTM
                     VRRSAPGGQW"
     gene            2299..>2564
                     /locus_tag="VFO88_03620"
     CDS             2299..>2564
                     /locus_tag="VFO88_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET9544641.1"
                     /translation="MSVAAALSPERVWERLAEIPDPEIPVISLVDLGVVRDVAVEGER
                     VRVEFTPTFLGCPALEVMQRLMADAVRELGGEPEVVVRTDDPWTT"
BASE COUNT          391 a          873 c          948 g          352 t
ORIGIN      
        1 ccttcgcggc cctcgcggaa gagcgcgcgg atccgctcct cgtagcggcg gcgctcggcg
       61 aggatctcgt cgcggcgctc cgcgtcgagc gagcgccact cgcgcacgaa gacggtcgcc
      121 acgtcgagct ggtcggccac gagccgcaga tgggcgcgaa gggcgaggcg gatcttctcc
      181 gtcgcgggcg cgcgctcggg cagctcgtcg agcgccgcgt ggaaggcccg cgcgccgacg
      241 agcatcgtct cgtagaggag ctcctgcttc ccggcgatgt gcgcgtagag cgagcccttc
      301 tggacgccga gcgccttcgc gatgtcgccg atcgacgtcg cgtggtagcc cttctcggcg
      361 aagagacggg ccgcttcctc ggagagctgt tgccggcggg gcgtgctaat ctcgtcaccc
      421 taacgatcgt tcggtaggag gggcaaatga cgccgcgtac ggaggaagcg ttcatcgagc
      481 acgtgcagtc cggcggccag gtcgaggcga ccgactggat gcccgacgag taccgcgcga
      541 agctcgtcaa gttcatcgag atgcacggga actcggagct catgggcgtc ctgcccgagc
      601 gcgagtggat cctccgcgcg cccacgctcc agcgcaagct cgcgctgacg gcgaaggtgc
      661 aggacgaggc aggccacgcg cagctcatct accgcgtcgt cgaggatctc ggcaagccgc
      721 gcgacgcgat cctcgaggac ctgatctcgg gcaagagcaa gttccacaac gtcttccact
      781 acccgacgaa gacctggggc gacgtcggcg tgatcgcctg gctcgtggac gccgcggcga
      841 tcatcagcca gaaggcgctc ctcaagtgct cgtacgcgcc gtacgcgcgg atcatgaaga
      901 agatctgctg ggaggagtcg ttccacatcc tccacggccg cgacgtcatc ctcgcgatgg
      961 tgaccggcac gccggagcag cgggagctcg tccaggaggc gctcgaccgg tggtggaagc
     1021 cgctcatgat gttccacggc aaccagatcc cggccgagaa ggacccgatg taccactggc
     1081 ggatcaagag ccagggcaac gaggaggcac gccagcagtt cctcgacggc tacgtccccc
     1141 agatccgcga gctcgggctc gagatcccgg atccgaagct ccgcagggac gaggagaccg
     1201 ggcgctggga gtacaccgag ccggactggg acgagctgag gcacgtcgtc accggccacg
     1261 gcccgatgac cgccgagcgg ctcgggttcc ggcgggcgtc gcgcgacgag accgcctggg
     1321 tgcgcgacgt cgtcctcgcc gaagcggcgt agagccatgg ccgtgtggga ggtcttccgc
     1381 caggagcgcg agggccagcc gttccagcac ggcgggtcgc tcgaggcgcc gaaccgcgag
     1441 ttcgccgacg cgtacgcgcg cgagttctac ggccgccgcg acgagtcgtt cgcgctctgg
     1501 atcgtcccgc gcgaggccgt cctggaggtg cacgagccct acgtcgcgcc ggtcttcgac
     1561 cgcgactacc gccgcgtcga cggctactcg atcaaggtca agctgaagga ggcccgtgag
     1621 ctcgcaggca ccgccgagag ctgacgagct cctcgcgctc gccgacgacg agctgatcct
     1681 cggctggcgc gactcggagt ggacggggat cgcgccgttc ctcgaggagg acgtcgcgtt
     1741 ctcgtcgatc gcgcagacgg agatcgggca cgcgcgcgcg ctctacgagc tcgcggcgcg
     1801 ggagctcggc gggacggcgg acacgctcgc gttcgaccgc cggccggacg agtaccgctc
     1861 ctcgccgctc gtcgagctcc ggctcgtgca cgactggggc cggacgatcg cgcgtcacat
     1921 cctctacgag ctcgccgacg cggtccgcgt ggccgagctc aaggagtcgg acgacgccga
     1981 cctcgccggg ctcgccgaga agatcgagcg cgaggaggcc taccacgtca tgcacgcgca
     2041 gatgtgggcc gaccggctcg cggagtcgga ggagggccgc gcgcggctcc aggccgggct
     2101 cgacgagctc tggccgtacg cactcgggct cgtcgagggc gggcagcggg agcggttcct
     2161 cgccgacgtg gccgagcggc tgccgttctt ctccccgccg gaggtcgagc cggtcgcgcg
     2221 gggcgagcac gtggacgagt gggccgagct ctgggaggag atgacgatgg tgcgccgctc
     2281 cgcgcccggg gggcagtggt gagcgtcgcc gccgcgctga gcccggagcg cgtctgggag
     2341 cgcctcgccg agatccccga cccggagatc ccggtcatct cgctcgtgga cctcggcgtc
     2401 gtgcgcgacg tcgccgtcga gggcgagcgg gtgcgcgtcg agttcacgcc gaccttcctc
     2461 ggctgccccg cgctcgaggt gatgcagagg ctcatggcgg acgcggtgcg cgagctcggc
     2521 ggcgagcccg aggtggtcgt gcgcacggac gacccgtgga cgac
//
LOCUS       DASTFC010000123         5220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_447003,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000123 DASTFC010000000
VERSION     DASTFC010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447003"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFO88_03625"
     CDS             complement(<1..1724)
                     /locus_tag="VFO88_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9544642.1"
                     /translation="MSWLIPQLRSRGVAVALVGAALAVVVQLTFGPELDWDPLLALAA
                     GLALAALAGAAGGLVAGIVTAAVGAGLFVAYGTGDTDIAWLTVPAWLVAGAAAGLASD
                     LWSRARSERGSARAELAALREAAPAALVTVGPDATVYAWNPAAERLFGLPAGEAIGAE
                     AGVLGPDVADALAQARDGGAPVDATIERKDEAGDAFRLLVRALAFGAPEGEHAVLGAL
                     DTSGSVKLERELRDAEARHEALARTLPAVHYVHPPGERGSLTFVGPQVKRLLGYEPEE
                     LVEGTIRLDDLVHADDRERVAEELRAGGQRPFRSEYRLVARDGRELPVRDEATTVRDA
                     NGRPLYVQGFLVDLSHRLELEAERERLGREREDARGEALARRQRLDLAVEAGELLAAA
                     ADAETALRRVADLAVRDFADWCAVDLTSDDEGLTRLTASHAEVIYDGESRAAGPEAVP
                     TEDVAEAAAGSEPILSPDGSRLLAPMVARGHTLGVLTFVRGARGRSYGADDLALAADV
                     ARRAALTLDAARLHRRVELEADAARVLAYVADGVFLVDRTGVIRLWNPAAEAVTGYSA
                     DAMIGRRPS"
     gene            complement(1819..2046)
                     /locus_tag="VFO88_03630"
     CDS             complement(1819..2046)
                     /locus_tag="VFO88_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544643.1"
                     /translation="MSDTWLWVIAALILFPLAGLLLMLALGASVAPADAVRRLWQRAT
                     MRRPVHRGVALPDEVPEEQPPPEDSPDRPGG"
     gene            complement(2043..2801)
                     /gene="narI"
                     /locus_tag="VFO88_03635"
     CDS             complement(2043..2801)
                     /gene="narI"
                     /locus_tag="VFO88_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HET9544644.1"
                     /translation="MTDRLDLLLWVALPYLALAIFVVGHVWRYRRGQLTWTSRSTQIL
                     ERRLLQVGSLLFHFGILAVIGGHVLGILVPSGVTEAVGIDHDTYHLISILAGGTAGIA
                     ATLGILVLVYRRVAIGRVRASTPRSDLVMYPVLLAALVTGMLATFGPNLLLGEYEYRE
                     TVAPWFRGVFAFSPDPELMTGAPFLFQAHAVTAWLLFAVWPFSRLVHAWSIPVSYLAR
                     SWIPFRSRDPRTALAVQRARAARASEAQGARRAP"
     gene            complement(2798..3376)
                     /gene="narJ"
                     /locus_tag="VFO88_03640"
     CDS             complement(2798..3376)
                     /gene="narJ"
                     /locus_tag="VFO88_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HET9544645.1"
                     /translation="MTRYTEIARLLLYPEAPLDAAPAGDRHVDAFLAATRGVPLDRLQ
                     DDYVRTFDFDKRTNLYLTYHLHGDGRRRGVELVRLKRRFREAGLELAEGELPDYLPVL
                     LEFAELEPEAGVAVLAGLRGSLELVRSALHERRSPYAHLLEAVVEGLPALTRRQRAEI
                     ERLAVDGPPTELVGLDSFTPPELEPFLAGGKR"
     gene            complement(3373..4893)
                     /gene="narH"
                     /locus_tag="VFO88_03645"
     CDS             complement(3373..4893)
                     /gene="narH"
                     /locus_tag="VFO88_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HET9544646.1"
                     /translation="MRVRAQVGMVMSLDKCIGCHTCSVTCKNVWTNRKGAEYIWFNDV
                     ETKPGVGYPKRWEDQEQWNGGWELDRKGKLRLKAGGKLRKLANIFYNPDLPTLEDYYE
                     PWTYDYEHLTSAPPSEHQPVARPRSQVDGRPLELQWGPNWEDDLAGSQVYARQDPNLR
                     AAAEEAVAFEYEQAFMFYLPRICEHCMNPSCVASCPSGAMYKREEDGIVLVDQEACRG
                     WRFCVSGCPYKKVYFNWATGKAEKCTFCYPRIEAGQPTVCSETCVGKIRYLGIVLYDA
                     DRVEEAASVPDEHDLLESQLSLVLDPDDPDVQERARADGIPEDWLEAARRSPVYALLC
                     RHRVALPLHPEFRTLPMVWYVPPLSPVLEFAEGPQGDGDPDDVFPAIDDLRIPISYLA
                     NLLAAGDEEPVRLALKRQAAMRRVMRERTFGAEPDPALAMEVGMTLADVEDLYRLLAI
                     AKYDDRYVIPQAHAETAGDLYAMQGSCGFDVTPAEGYGISAGEPPVDLRRMLEDRP"
     gene            complement(4893..>5220)
                     /locus_tag="VFO88_03650"
     CDS             complement(4893..>5220)
                     /locus_tag="VFO88_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET9544647.1"
                     /translation="ERIGVVDNDWVEVFNRNGVIACRAVVSHRVPDGISLMYHSQDRH
                     VNVPLSELSGTRGGTDNSVTRISIKPTLLVGGYAQLSWGFNYYGPTGPQRDEVVVVRK
                     LEEVVY"
BASE COUNT          711 a         1862 c         2002 g          645 t
ORIGIN      
        1 gagggccgcc tcccgatcat cgcgtcggcg gagtagccgg tgaccgcctc ggcggcgggg
       61 ttccagaggc ggatcacgcc ggtgcggtcg acgaggaaga cgccgtccgc gacgtacgcg
      121 aggacgcgtg ccgcgtccgc ctcgagctcg acccgccggt ggaggcgggc ggcgtcgagc
      181 gtgagcgccg cgcggcgggc gacgtccgcg gcgagcgcga ggtcgtcggc gccgtacgag
      241 cgcccccggg ccccgcggac gaaggtgagg acgccgagcg tgtgcccgcg cgcgaccatc
      301 ggcgcgagga ggcgcgatcc gtccggggag aggatgggct cgctgcccgc ggccgcttcc
      361 gcgacgtcct ccgtgggcac ggcctcgggc ccggcggccc gcgactcgcc gtcgtagatg
      421 acctcggcgt gcgaggccgt gagcctcgtg aggccctcgt cgtcgctcgt caggtcgacc
      481 gcgcaccagt ccgcgaagtc ccgcacggcg aggtcggcga cgcggcgcag ggcggtctcg
      541 gcgtccgcgg cggcggcgag gagctctccg gcctcgacgg cgaggtcgag ccgttgccgg
      601 cgcgcgagcg cctcgccgcg cgcgtcctcc cgctcgcggc cgagccgctc gcgctcggcc
      661 tcgagctcca ggcggtggct caggtcgacg aggaagccct ggacgtagag cggccggccg
      721 ttcgcgtcgc ggacggtggt cgcctcgtcg cggacgggga gctcgcgccc gtcgcgcgcc
      781 acgagccggt actcggagcg gaacggccgc tgcccgcccg cgcggagctc ctccgcgacg
      841 cgctcccggt cgtcggcgtg gacgaggtcg tcgagccgga tcgtgccctc gacgagctcc
      901 tcgggctcgt agccgaggag ccgcttgacc tgcggcccga cgaacgtgag cgagccgcgc
      961 tcgccgggcg gatggacgta gtggacggcg ggcagcgtcc gcgcgagcgc ctcgtggcgc
     1021 gcctcggcgt cgcggagctc gcgctcgagc ttgaccgagc cgctcgtgtc gagcgcgccg
     1081 aggacggcgt gctcgccctc gggagccccg aacgcgagcg cgcgcacgag gaggcggaac
     1141 gcgtcgcccg cctcgtcctt ccgctcgatc gtcgcgtcga ccggggcgcc gccgtcacgc
     1201 gcctgcgcga gcgcgtcggc gacgtcgggg ccgaggacgc cggcctccgc gccgatcgcc
     1261 tcgccggcgg ggaggccgaa gagccgctcg gcggcggggt tccaggcgta cacggtcgcg
     1321 tcggggccga ccgtgacgag cgcggccggc gcggcctcgc gcagcgcggc gagctcggcc
     1381 cgggccgagc cgcgctcgct ccgcgcgcgg ctccagaggt cggacgcgag cccggcggcc
     1441 gcgccggcga cgagccaggc cggcacggtg agccacgcga tgtccgtgtc ccccgtcccg
     1501 taggcgacga agaggcccgc cccgaccgcg gccgtgacga tgcccgcgac gagcccgccg
     1561 gccgcgccgg cgagggccgc gagcgcgagc ccggcggcga gggcgaggag cggatcccag
     1621 tcgagctccg gcccgaacgt gagctggacg acaacggcga gcgccgcgcc gacgagagcg
     1681 acggcgacgc cacggctccg cagctgcggg atgagccagg acatccgaac cttccagaag
     1741 acgggctacc agtgaagcac gcttcgcgcg ccgttccaag ttcaccgcac ggaggccgat
     1801 acctgcggca gcccggcgtc agccgcccgg acggtcgggg gagtcctccg gcggcggctg
     1861 ctcctcgggc acctcgtccg gaagcgcgac gccccggtgg accggacgcc gcatcgtcgc
     1921 ccgctgccag agacgccgaa cggcgtcggc gggggcgacc gacgcgccga gcgcgagcat
     1981 gaggagcagg ccggcgagcg ggaacaggat gagcgccgcg atgacccaga gccaggtgtc
     2041 gctcacggcg cgcgccgcgc tccctgcgcc tcggacgcgc gggcggcgcg ggcccgctgg
     2101 acggcgagcg ccgtgcgcgg gtcgcggctc cggaagggga tccagctccg tgcgagatag
     2161 gagaccggga tgctccacgc gtggacgagg cgcgagaacg gccagacggc gaagaggagc
     2221 cacgccgtga ccgcgtgcgc ctggaagagg aacggcgccc ccgtcatgag ctccgggtcg
     2281 ggcgagaacg cgaagacgcc ccggaaccac ggcgcgacgg tctcgcggta ctcgtactcg
     2341 ccgagcagga ggttcggccc gaacgtggcg agcatcccgg tgacgagcgc cgcgaggagc
     2401 accgggtaca tgacgaggtc gctgcgcggg gtcgaggccc gcacgcggcc gatcgcgacg
     2461 cgccggtaga cgaggacgag gatcccgagc gtcgccgcga tcccggccgt cccgcccgcg
     2521 aggatcgaga tcaggtggta cgtgtcgtgg tcgatcccga ccgcctccgt cacccccgac
     2581 gggacgagga tcccgagcac gtggccgccg atcacggcga ggatcccgaa gtggaagagc
     2641 aggctgccca cctggaggag ccgccgttcc aggatctgcg tcgagcggga cgtccacgtg
     2701 agctgcccgc gccggtagcg ccagacgtgc ccgacgacga agatcgcgag ggcgagatac
     2761 ggcagcgcga cccagaggag caggtcgagg cggtcggtca cctcttcccc ccggcgagga
     2821 acggctcgag ctccggcggc gtgaacgagt cgaggccgac gagctcggtc ggcgggccgt
     2881 cgaccgcgag ccgctcgatc tcggcgcgct ggcggcgcgt gagcgcgggg agcccctcga
     2941 cgaccgcctc gagcaggtgc gcgtacgggc tccggcgctc gtgcagggcg gagcggacga
     3001 gctcgagcga gccgcgcagc ccggcgagga cggcgacgcc tgcctcgggc tcgagctccg
     3061 cgaactcgag caggacgggg aggtagtcgg gcagctcgcc ctcggcgagc tcgagcccgg
     3121 cctcgcggaa gcgccgcttg agccggacga gctcgacccc gcgccgccgg ccgtcgccgt
     3181 ggaggtggta cgtgaggtag aggttcgtcc gcttgtcgaa gtcgaacgtg cgcacgtagt
     3241 cgtcctggag gcggtcgagc ggaacgccgc gcgtcgcggc gaggaaggcg tccacgtgcc
     3301 ggtcgccggc cggcgccgcg tcgagcgggg cctcggggta gaggagcagg cgcgcgatct
     3361 ccgtgtaccg cgtcacggcc ggtcctcgag catccgcctg aggtcgaccg gcggctcgcc
     3421 cgcgctgatc ccgtagccct ccgcgggcgt cacgtcgaac ccgcagctcc cctgcatggc
     3481 gtagaggtcg ccggccgtct ccgcatgcgc ctgcgggatc acgtagcggt cgtcgtactt
     3541 cgcgatcgcg agcaggcggt agaggtcctc gacgtccgcg agcgtcatcc cgacctccat
     3601 cgcgagcgca gggtccggct cggcgccgaa cgtgcgctcg cgcatcacgc gccgcatcgc
     3661 cgcctggcgc ttgagcgcga ggcgcaccgg ctcctcgtcg cccgccgcga ggaggttcgc
     3721 gaggtagctg atcgggatcc gcaggtcgtc gatcgccggg aagacgtcgt ccgggtcgcc
     3781 gtcgccctgg gggccctccg cgaactcgag cacgggggag agcggcggga cgtaccagac
     3841 catcggcagc gtccggaact ccgggtggag cggcagcgcg acccggtgcc ggcagaggag
     3901 cgcgtagacc ggcgagcgcc gcgccgcctc gagccagtcc tccgggatgc cgtcggcgcg
     3961 cgcccgctcc tgcacgtccg ggtcgtccgg gtcgaggacg agcgagagct gcgactcgag
     4021 caggtcgtgc tcgtcgggga cggaggcggc ctcctcgacc cggtctgcgt cgtagaggac
     4081 gatcccgagg tagcggatct tcccgacgca ggtctcggag cagacggtcg gctggccggc
     4141 ctcgatgcgc gggtagcaga aggtgcactt ctccgccttg ccggtcgccc agttgaagta
     4201 gaccttcttg tacgggcagc cggagacgca gaagcgccag ccgcggcagg cctcctggtc
     4261 gacgagcacg atcccgtcct cctcgcgctt gtacatcgcg cccgagggac aggacgcgac
     4321 gcacgagggg ttcatgcagt gctcgcagat ccgcgggagg tagaacatga acgcctgctc
     4381 gtactcgaag gcgacggcct cctcggcggc ggcgcgcagg ttcgggtcct ggcgcgcgta
     4441 gacctgcgag ccggcgaggt cgtcctccca gttcggcccc cactgcagct cgagcggccg
     4501 gccgtccacc tgcgagcggg ggcgcgcgac cggctggtgc tcggagggcg gcgcgctcgt
     4561 caggtgctcg tagtcgtacg tccacggctc gtagtagtcc tcgagcgtgg ggaggtccgg
     4621 gttgtagaag atgttcgcga gcttgcggag cttgccgccc gccttgagcc ggagcttccc
     4681 cttgcggtcg agctcccagc cgccgttcca ctgctcctgg tcctcccagc gcttcggata
     4741 gccgacgccc ggcttcgtct cgacgtcgtt gaaccagatg tactcggcgc ccttgcggtt
     4801 cgtccagacg ttcttgcacg tgaccgagca cgtgtggcag ccgatgcact tgtcgaggct
     4861 catgaccatc ccgacctgcg cgcggactct cactagtaga cgacctcctc gagcttgcgc
     4921 acgaccacga cctcgtcccg ctggggcccg gtcggcccgt agtagttgaa cccccaggag
     4981 agctgcgcgt agccgccgac gagcagcgtc ggcttgatcg agatgcgcgt gacggagttg
     5041 tccgtcccgc cgcgcgtgcc cgagagctcg gagagcggca cgttcacgtg ccggtcctgc
     5101 gagtggtaca tgagcgagat cccgtccggg acgcggtgcg agacgaccgc gcggcacgcg
     5161 atcacgccgt ttcggttgaa gacctcgacc cagtcgttgt cgacgacgcc gatccgctcc
//
LOCUS       DASTFC010000124        10122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1600697,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000124 DASTFC010000000
VERSION     DASTFC010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600697"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1003)
                     /locus_tag="VFO88_03655"
     CDS             complement(17..1003)
                     /locus_tag="VFO88_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9544648.1"
                     /translation="MTPLLELRGVEVGFPVRGGRVARAVDGVDLTVEPGEVLALVGES
                     GCGKTTLARTIVGLERPDAGEVRFEGEPVARRGRALRAFRRRVQMVFQDPAGALNPRQ
                     TIYEAVAEGLRIQKTPGNEEELVAQALARAGLRPPERFFRLYPYEISGGQRQRVVIAG
                     AMAVGPSLLVADEPVSSLDASVRGEILELMLGLVRETGVSILVVTHDLGLAWNVADRV
                     AVMYLGRIVEIGTTEELLGDPKHPYTAALLSVVPEAGGREPLILSGETPDPTRIPSGC
                     RFHPRCPLYASGEAARLGIEERCRGEDPALAPARGGPQLAACHATALTEAAA"
     gene            complement(1000..1989)
                     /locus_tag="VFO88_03660"
     CDS             complement(1000..1989)
                     /locus_tag="VFO88_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9544649.1"
                     /translation="MSAAPRPEAPLLAVRDLAVTYAGAGGGVPAVRGVSLDLDPGETL
                     GLAGESGCGKSTLAGAILRLLPRGTEVTGQVLLDGEDVLAMRPGRLRAVRWTEASIVL
                     QGALHSLNPVQRIGAQIGEAIELHEPKLGRGAVRARVAELLELVGIPAGRASDYPHRL
                     SGGQRQRVLIALALACRPRLLIADEPTTALDVMVQAQVLRLLARLQEELGLAVLFVTH
                     DLSTLAAVCRRIAVMYAGRIVEEGPSDEVFRAPAHPYTRALAAAFPVIGDPAFRRHPS
                     GLPGDPPDPRELPSGCPFHPRCPLAQPECPDVDVRLRPAGAGRAAACVLVEPA"
     gene            complement(1986..2900)
                     /locus_tag="VFO88_03665"
     CDS             complement(1986..2900)
                     /locus_tag="VFO88_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9544650.1"
                     /translation="MRGAVATSATRVAWARRRRAVAGAWGDYRRSRTGMIGLAVLVLA
                     GLVALGAPLLADEAELRAVNAIDNPSWAPPSEFGPLGTDNLGRSVWAQFVWGSRISLL
                     VGLAATVIAIVIGTVVGVSAGFVGGRFGGLLMRGTEWFLVIPFLPLAIVLAAVLGPSI
                     ENIILVIGITSWPGTARLIRAQVLTLKERLYVDRSRAFGASNRHLMGRHILPNVMPLV
                     LANTTLTVPIAILSETTLSFLGLGDPSRASWGKMLEEAFHAGAITREAWWYFLPPGLG
                     IMLVVLAFTLCGQALEAILDPRLRERRS"
     gene            complement(2897..3862)
                     /locus_tag="VFO88_03670"
     CDS             complement(2897..3862)
                     /locus_tag="VFO88_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9544651.1"
                     /translation="MNRRRVIGKILASVGTLAFVVVFNFFLFRVVESDPVGNLYRGRN
                     LSAEQREELRREFGLDGSQAEQFARYVAQTAQLNFGRSYLTNEPVIDEIKRRAWPTVA
                     LVGVSALLSALIGILIGVVAAWRRGSKTDYVSTGFTMTTYSMPDFWLGMLLLLVFAAG
                     LGWFPVGGIVDPQTGGGLERLLDQAHHMVLPAATLTLAYLGEYALIMRSSLLDTMRED
                     YLLLARAKGLRDVAVRNRHAVPNALLPVTTLLAINIGFVLSGAIAVETIFSWPGLGLA
                     TFEALSGPDLPMLQGLFLVFSAAVIVANLAADLLYSYLDPRVRTA"
     gene            complement(3884..5689)
                     /locus_tag="VFO88_03675"
     CDS             complement(3884..5689)
                     /locus_tag="VFO88_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9544652.1"
                     /translation="MAAPRLLVAIVATLVILTAAGGATAQERLILTVGIPNDIGSLNP
                     IVGVEVPDYEVWNLHYATLTDKAAEDFETIPGLAESWEGSEDGLTWTYTLREGLLWSD
                     GEPLTADDVVFTINRAREEAWLNYDSTVANITASAPDERTVVLESSVPDPKLPTMDVY
                     ILPEHIWGELGPREITRYDGLDGVGSGPFTIAELRRGEFWRLEANENYWAGRPAIDEV
                     VFRLFNNTDAMVAALTAGEIDAAHYVPEGSFQQLSETEGIETIEGQQGGFDELAVNGG
                     DGLADPHPALLDLRVRQAVAHAIDEETIIDRVLEGLGSPSEAMSPSANPEWIPEIPDE
                     ERFDFDLDRARELLDEGGYEDTDGDGIREMPGGGEPLDFVFAVRTESQPAPGIAEFVS
                     GWLEEIGIAVTQRPMNDSRLLEVIGRGNYDMFVWGWVPFVDPDPMLSYFKCDQIASDP
                     EDPTNYYNDANLCDPEYDRLYEEQKVELDHERRVEIVHEMLRRFYSHAVYHVLYLSPD
                     LQAYRTDRFTGWVRQPADVGPVMFSNSSPSYALLRPVSEEDEAAGGGGEAAAATDDDG
                     GMSTGTLVALVALALLVVAAAVWILRRRRTVDERD"
     gene            complement(5710..7551)
                     /locus_tag="VFO88_03680"
     CDS             complement(5710..7551)
                     /locus_tag="VFO88_03680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9544653.1"
                     /translation="MPEPVAHWIGKDERGGIPVPARPDVRPPPHWRLEAIAAVERPRS
                     LTLGADGRTAVYIQDRDTSDVWLLDLERGVPERLTSGREPAPYWEDVEPRLSPDGSQV
                     AYGDEGAVWIVPTAGGPPRRLAKGSSPVWLDDARLAITVERDRATRLAVVDVHDPWPR
                     RLARHDDHGDEGSAAVSPDGREVAFVFTPRDDLKRSEIRVADLATGETRALTGTPGMQ
                     DRSPAWSPDGATLAYVSERPGWYELHLVERDGSGDRQLTHEEADFSEPRWHPGGTRLV
                     AVRGRRNRFDLVTVDTATGAVETLAPGGAWGAPHWTADGEILGTYEDHATPPELRLVR
                     PGAEPRTLLAPSPRAIRRAPHVAPEEVAYTSFDGLEIPAFLFRPRDASPERPAPAVVY
                     PHGGPTDAYGDHWDGHAQYFLDKGYAWLAPNFRGSTGYGREFERRNHGVWGVDDTKDC
                     LAAADFLRGLDWVDGGRLAIFGGSYGSYMALCAAVDDDEGRFRCAVAKYGDCDILTSW
                     AQGDREGVQDLERMMGHPETAREAYLRGSPIHRLERLDVPILVAHGERDERVHPKQSE
                     ELVAGLRRLGKTFEYVTYPTEGHGFLRAGPQVDFYRRLERFLDWSLL"
     gene            complement(7544..8614)
                     /locus_tag="VFO88_03685"
     CDS             complement(7544..8614)
                     /locus_tag="VFO88_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET9544654.1"
                     /translation="MPLGLPVVWSDRHRLHDPGGEVWVGVRMPAAEVPARAEAIRDAL
                     AADGARIVGADPHPDERLLAVHDADLLDYLARAWADWEAAGLPDDPGQDRVVPYVFAH
                     PGLTETPAVPAATWARPGYFAYDTMTLVGPGTWEAARAAVDAALTAADLVLAGTPAAY
                     ACCRPPGHHVTRRAHGGSCYLNNAAAAAALLRDELGRVAVLDVDAHHGNGTQSIFWED
                     PDVLTGSVHVDPAAGWFPHYLGFAGETGAGDGAGTNLNLPLAPGTGDADWVEAVARLA
                     AWARDGGAQALVVALGVDAAAGDPESPLAVSPDGFREGGRALGALGLPTVVVQEGGYD
                     LDALGGLVLETLTGLEEGLAHA"
     gene            8638..9657
                     /locus_tag="VFO88_03690"
     CDS             8638..9657
                     /locus_tag="VFO88_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET9544655.1"
                     /translation="MSTLREHGIRIGELEPGPANAITDVPGFRVGHVTVRRDEPEPPE
                     GRGVARTGVTVLVPPRLPLPAGAAVLNGAGELTGFLQVSEWGVVETPVYLTGTMAVGR
                     VFDGAVAAAVAADPAVGVETVVIPVVGECDDSFLSEARVVQVEAADAGRALAATADGP
                     VEQGAVGAGTGMIAFGWKGGIGSASRVVPGRGTLGVLVLANFGTRGQLRVDGVVVGRA
                     LPPDPHERGPAGSCIALVATDAPLAAPQLERLARRAGLGLARTGSVAHHGSGEIFLAL
                     SSGEGETADRDLDALFAATVETTEEAVLNALWSAATTTGREGRVVEALPHDETVAALR
                     AARRN"
     gene            9667..>10122
                     /locus_tag="VFO88_03695"
     CDS             9667..>10122
                     /locus_tag="VFO88_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9544656.1"
                     /translation="MAQREVVQLDRGRIAELTEREEKRLNERTQASRALYERGRTSLA
                     GGVPSSYHLRDPWPIYLDRGQGPRVWDVDGNEMLDFHNGFGSMVQGHAHPAVAAAVRE
                     RLDKGTHFAAPTEDGIVVAEELARRFRLPKWRYTNSGSESTMDAIRIARA"
BASE COUNT         1356 a         3696 c         3822 g         1248 t
ORIGIN      
        1 cggttcgagc ggacgatcag gcggctgctt cagtgagcgc ggtcgcgtgg caggccgcga
       61 gctgcggccc gccgcgcgcc ggcgcgagcg cgggatcctc cccgcggcac cgctcctcga
      121 ttccgagccg cgccgcctcg cccgacgcgt agagggggca gcgcgggtgg aagcggcagc
      181 cggaggggat ccgcgtgggg tcgggcgtct cgccggagag gatcaacggc tcgcgcccgc
      241 ccgcctccgg cacgaccgac aggagcgccg cggtgtacgg gtgcttcggg tcgccgagca
      301 gctcctccgt cgtcccgatc tcgacgatcc gcccgaggta catgaccgcc acgcggtcgg
      361 cgacgttcca cgcgagcccg aggtcgtgcg tcacgacgag gatcgagacg cccgtctcgc
      421 gcacgaggcc gagcatgagc tcgaggatct ccccgcgcac cgaggcgtcg aggctcgaga
      481 ccggctcgtc ggcgacgagc aggctcggcc cgaccgccat cgcgccggcg atcacgacgc
      541 gctgccgctg accccccgag atctcgtacg ggtagaggcg gaagaaccgc tccggcggcc
      601 gtagcccggc gcgcgcgagc gcctgcgcga cgagctcctc ctcgttcccg ggcgtcttct
      661 ggatccgcag gccctcggcg acggcctcgt agatcgtctg ccgcgggttc agcgcgccgg
      721 cgggatcctg gaagaccatc tggacgcgcc gccggaaggc tcgcagcgcc cggccgcgcc
      781 gcgcgacggg ctcgccctcg aagcgcacct cgccggcgtc cggccgctcg agcccgacga
      841 tcgttcgcgc aagcgtcgtc ttgccgcagc cggactcgcc gacgagcgcg agcacctcgc
      901 ccggctcgac cgtcaggtcg acgccgtcca cggcccgtgc gacccggccg ccgcggacgg
      961 ggaagccgac ctcgacgccg cgcagctcga ggagcggcgt cacgcgggct ccacgaggac
     1021 gcaggccgcc gcccgcccgg cgccggccgg gcgcagccgg acgtcgacgt cgggacactc
     1081 gggctgcgcg agcgggcagc gcgggtggaa cgggcagccg gacggaagct cgcgcgggtc
     1141 gggcggatcg ccgggcagcc cggacggatg ccgccggaac gcggggtcgc cgatcaccgg
     1201 gaacgccgcg gccagcgccc gcgtgtacgg gtgggccggc gcgcggaaga cctcgtcgct
     1261 cggaccctcc tcgacgatcc gcccggcgta catgacggcg atccgccggc agacggcggc
     1321 gagcgtggag aggtcgtgcg tgacgaagag gacggccagg ccgagctcct cctgcagccg
     1381 cgcgagcagg cggagcacct gcgcctgcac catcacgtcg agcgccgtgg tcggctcgtc
     1441 cgcgatgagg agccgcggcc ggcaggcgag cgcgagcgcg atcagcaccc gctggcgctg
     1501 cccgcccgag agccggtgcg ggtaatcgga ggcgcgcccg gccgggatcc cgacgagctc
     1561 gagcagctcg gccacgcggg cgcgcacggc accgcgcccg agcttcggct cgtgaagctc
     1621 gatcgcctcg ccgatctgcg cgccgatccg ctgcaccggg ttcagggagt ggagcgcgcc
     1681 ctggaggacg atcgaggcct ccgtccagcg cacggcccgc aggcgcccgg gcctcatcgc
     1741 cagaacgtcc tcgccgtcga ggagcacctg acccgtgacc tccgtccccc gggggagcag
     1801 ccgcaggatc gccccggcga gcgtcgactt gccgcagccc gactcccccg cgagcccgag
     1861 cgtctcgccc gggtcgaggt cgaggctgac gccgcgcacc gcgggcacgc cgccgcccgc
     1921 gcccgcgtag gtgacggcga ggtcgcgcac cgcgaggagc ggcgcctccg gccgcggcgc
     1981 ggcgctcacg accgccgctc ccgcaggcgc gggtcgagga tcgcctcgag cgcctggccg
     2041 cagagggtga acgcgagcac gacgagcatg atcccgagcc ccggcgggag gaagtaccac
     2101 caggcctcgc gcgtgatcgc gcccgcgtgg aacgcctcct cgagcatctt cccccacgac
     2161 gcgcgcgacg ggtcgccgag gccgaggaac gagagcgtcg tctcggagag gatcgcgatc
     2221 gggacggtga gcgtcgtgtt cgcgaggacg agcggcatga cgttcgggag gatgtgtcgc
     2281 cccatcagat ggcggttcga cgcgccgaac gcgcggctgc ggtcgacgta gagcctctcc
     2341 ttcagcgtga gcacctgcgc gcggatgagc cgggccgtcc ccggccacga ggtgatcccg
     2401 atcacgagga tgatgttctc gatcgagggc ccgagcaccg cggcgagcac gatcgcgagc
     2461 ggcaggaagg ggatgacgag gaaccactcc gtgccgcgca tgagcagccc gccgaagcgc
     2521 ccgccgacga agccggccga gacgcccacg accgtcccga tcacgatcgc gatcaccgtc
     2581 gcggcgagcc cgacgagcag gctgatccgc gatccccaga cgaactgcgc ccagacgctg
     2641 cgcccgaggt tgtccgttcc gaggggcccg aattcgctcg gcggcgccca cgaggggttg
     2701 tcgatcgcgt tcaccgcgcg cagctccgcc tcgtcggcga ggagcggcgc gcccagcgcg
     2761 acgagccccg cgaggacgag gaccgcgagc ccgatcatcc cggtccggct ccgccggtag
     2821 tccccccacg caccggcgac cgcgcggcgt cggcgtgccc aggcgacccg ggtcgcggag
     2881 gtggcgaccg cgcccctcac gccgtccgca cccgcgggtc gaggtacgag tagagaagat
     2941 cggcggcgag gttcgcgacg atcacggccg cgctgaagac gaggaagagc ccctggagca
     3001 tcggcaggtc gggcccggag agcgcctcga acgtcgcgag cccgagcccc ggccaggaga
     3061 agatcgtctc gaccgcgatc gcgcccgaga ggacgaaccc gatgttgatc gcgaggagcg
     3121 tcgtcacggg caggagggcg ttcggcacgg cgtggcggtt ccggacggcg acgtcgcgca
     3181 gccccttcgc gcgcgcgagg agcaggtagt cctcgcgcat cgtgtcgagg agcgacgagc
     3241 gcatgatgag cgcgtactcg ccgaggtagg cgagcgtgag cgtcgcggcc ggcagcacca
     3301 tgtggtgcgc ctggtcgagc agccgctcga gcccgccgcc ggtctgcggg tcgacgatcc
     3361 cgccgacggg gaaccagccg agcccggcgg cgaagacgag caggaggagc atcccgagcc
     3421 agaagtccgg catcgagtag gtcgtcatcg tgaaccccgt ggagacgtag tcggtcttgg
     3481 agccgcgcct ccaggcggcg acgaccccga tcaggatccc gatcagggcc gagaggagcg
     3541 ccgagacccc gacgagcgcg acggtcggcc acgcccgccg cttgatctcg tcgatgaccg
     3601 gctcgttcgt caggtacgag cggccgaagt tcagctgcgc ggtctgcgcg acgtagcgcg
     3661 cgaactgctc cgcctgcgag ccgtcgagcc cgaactcgcg gcggagctcc tcacgctgct
     3721 cggccgagag gttccggccg cggtagaggt tcccgaccgg gtccgactcg acgacccgga
     3781 agaggaagaa gttgaagacg acgacgaacg cgagcgtccc gaccgacgcg aggatcttgc
     3841 cgatgacgcg ccggcgattc acgagccccc gcgcccgggc cggtcagtcg cgctcgtcga
     3901 ccgttcgcct gcggcgcagg atccacacgg ccgccgcgac cacgagcagc gcgagcgcga
     3961 cgagggcgac gagcgttccg gtgctcatcc cgccgtcgtc gtccgtggcg gcggccgcct
     4021 cgccgccgcc cccggccgcc tcgtcctcct ccgagaccgg tcgcaggagc gcgtaggagg
     4081 gcgaggagtt cgagaacatg accggcccga cgtccgcggg ctggcgcacc cagccggtga
     4141 agcggtcggt gcggtacgcc tgcaggtccg ggctcaggta gagcacgtgg tagacggcgt
     4201 gcgagtagaa gcgccgcagc atctcgtgga cgatctcgac gcgccgctcg tggtcgagct
     4261 cgaccttctg ctcctcgtag agccggtcgt actcggggtc gcagaggttc gcgtcgttgt
     4321 agtagttcgt cgggtcttcc gggtcggagg cgatctggtc gcacttgaag tacgagagca
     4381 tcgggtcggg gtcgacgaac ggcacccagc cccacacgaa catgtcgtag ttcccgcggc
     4441 cgatcacctc gagcagccgt gagtcgttca tcggacgctg cgtgaccgcg attccgatct
     4501 cctcgagcca gccggagacg aactccgcga tccccggtgc gggctgcgac tcggtgcgga
     4561 cggcgaagac gaagtcgagc ggctcgccgc cgccgggcat ctcacggatg ccgtcgccgt
     4621 ccgtgtcctc gtagccgcct tcgtcgagga gctcgcgcgc gcggtcgagg tcgaagtcga
     4681 agcgctcctc gtccgggatc tcggggatcc actccgggtt cgcggacggg ctcatcgcct
     4741 cgctcggcga gccgagccct tccaggacgc ggtcgatgat cgtctcctcg tcgatcgcgt
     4801 gggcgaccgc ctggcggacg cgcaggtcga ggagcgccgg gtgcgggtcg gcgagcccgt
     4861 cgccgccgtt caccgcaagc tcgtcgaacc cgccctgctg gccctcgatc gtctcgatcc
     4921 cctccgtctc ggaaagctgc tggaacgagc cctcggggac gtagtgcgcg gcgtcgatct
     4981 cgcccgccgt cagggcggcg accatcgcgt ccgtgttgtt gaagaggcgg aagacgacct
     5041 cgtcgatcgc cggccgcccg gcccagtagt tctcgttcgc ctcgagccgc cagaactcgc
     5101 cgcgccggag ctcggcgatc gtgaacggcc ccgagccgac gccgtcgagc ccgtcgtagc
     5161 gcgtgatctc gcgcgggccg agctcgcccc agatgtgctc cgggaggatg tagacgtcca
     5221 tcgtcgggag cttcgggtcg gggacgctcg actcgagcac gacggtgcgc tcgtcgggcg
     5281 cggaggcggt gatgttcgcc acggtcgagt cgtagttcag ccacgcctcc tcgcgcgcgc
     5341 ggttgatcgt gaagacgacg tcgtccgcgg tgagcggctc gccgtccgac cagaggaggc
     5401 cctcgcgcag cgtgtacgtc caggtgagcc cgtcctccga gccctcccac gactcggcga
     5461 ggccggggat cgtctcgaag tcctccgcgg ccttgtccgt cagggtcgcg tagtggaggt
     5521 tccagacctc gtagtccggc acctcgaccc cgacgatcgg gttcaggctg ccgatgtcgt
     5581 tcgggatccc gacggtgagg atgagccgtt cctgggcggt cgcgcctccc gcggcggtga
     5641 ggatgacgag cgtcgcgacg atcgcaacga gcaggcgggg ggcggccatc gcgggattat
     5701 ggcgtgcgcc tacagcaaag accagtccag gaatcgctcg agccgccggt agaagtcgac
     5761 ctgcggcccg gcgcgcagga agccgtgccc ctccgtcggg taggtcacgt actcgaacgt
     5821 cttcccgagc cggcggagcc cggcgacgag ctcctcggac tgcttcggat ggactcgctc
     5881 gtcccgctcg ccgtgcgcga cgaggatcgg gacgtcgagc cgctcgagcc ggtggatcgg
     5941 tgacccgcgg aggtacgcct cccgggcggt ctcggggtgg cccatcatcc gctcgaggtc
     6001 ctggacgccc tcccggtcgc cctgcgccca cgaggtgagg atgtcgcagt cgccgtactt
     6061 cgcgaccgcg cagcggaagc ggccttcgtc gtcgtccacg gcggcgcaga gcgccatgta
     6121 cgagccgtag cttccgccga agatcgcgag gcgcccgccg tcgacccagt cgagcccgcg
     6181 gaggaagtcg gcggcggcga ggcagtcctt cgtgtcgtcc acgccccaga cgccgtggtt
     6241 tctccgctcg aactcgcgcc cgtagccggt cgacccgcgg aagttcggcg cgagccaggc
     6301 gtagcccttg tcgaggaagt actgcgcgtg gccgtcccag tggtcgccgt acgcgtcggt
     6361 cggcccgccg tgcgggtaga cgaccgcggg cgccggccgc tccgggctcg cgtcgcgcgg
     6421 acggaagagg aacgccggga tctcgaggcc gtcgaacgac gtgtacgcga cctcctccgg
     6481 cgcgacgtgc ggggcgcggc ggatcgcgcg cggcgacgga gcgaggagcg tgcgcggctc
     6541 ggcgcccggc cggacgagcc ggagctcggg cggggtcgcg tgatcctcgt acgtcccgag
     6601 gatctcgccg tccgcagtcc agtgcggggc gccccaggcg ccgccggggg cgagcgtctc
     6661 gaccgcgccg gtcgccgtgt cgacggtcac gaggtcgaag cggttgcgcc gcccgcggac
     6721 cgcgacgagc cgcgtcccgc cggggtgcca gcgcggctcg gagaagtcgg cctcctcgtg
     6781 cgtgagctgc cggtcgccgc ttccgtcgcg ctcgacgagg tggagctcgt accagccggg
     6841 ccgctccgag acgtacgcga gcgtcgcgcc gtcgggcgac cacgccgggc ttcggtcctg
     6901 cattccgggc gtgccggtga gcgcgcgcgt ctcgccggtc gcgaggtcgg cgacgcggat
     6961 ctcgctccgc ttgaggtcgt cgcgcggcgt gaagacgaac gcgacctcgc ggccgtcggg
     7021 cgagacggcg gcgcttccct cgtcgccgtg gtcgtcgtgc cgggcgaggc ggcgcggcca
     7081 ggggtcgtgc acgtcgacga ccgcgaggcg cgtcgcccgg tcgcgctcga ccgtgatcgc
     7141 gaggcgggcg tcgtcgagcc agaccgggct cgagcccttc gcgaggcggc gcggcgggcc
     7201 gcccgccgtc ggcacgatcc agacggcgcc ctcgtcgccg tacgcgacct gcgacccgtc
     7261 cggcgagagg cgcggctcga cgtcctccca gtacggcgcc ggctcgcgcc cgctcgtcag
     7321 gcgctccggg acgccgcgct cgaggtcgag gagccagacg tcggaggtgt cgcgatcctg
     7381 gatgtagacg gccgtccgcc cgtccgcgcc gagcgtgagc gagcgcggcc gctcgaccgc
     7441 ggcgatcgcc tcgaggcgcc agtgcggcgg gggccgcacg tccggccggg cgggcacggg
     7501 gatgccgccg cgctcgtcct tgccgatcca gtgcgcgacc ggctcaggca tgcgcgaggc
     7561 cctcctcgag gccggtgagc gtctccagga cgaggccgcc gagcgcgtcg aggtcgtacc
     7621 cgccctcctg gacgacgacc gtaggcaggc cgagagcgcc gagcgcgcgg ccgccctcgc
     7681 ggaagccgtc cggcgagacg gcgagcgggc tctcagggtc gcccgcggcc gcgtcgacgc
     7741 cgagcgcgac gacgagcgcc tgcgccccgc cgtcccgcgc ccaggccgcg aggcgcgcga
     7801 cggcctcgac ccagtcggcg tcgccggtgc ccggcgcgag cgggaggttc aggttcgtgc
     7861 ccgcgccgtc cccggcgccg gtctcgccgg cgaagccgag gtagtgcggg aaccagccgg
     7921 cggccggatc gacgtggacg gagcccgtca gcacgtcggg atcctcccag aagatcgact
     7981 gcgtcccgtt gccgtggtgc gcgtcgacgt cgaggacggc gacgcggccg agctcgtcgc
     8041 ggaggagcgc cgcggccgcg gcggcgttgt tcaggtagca ggagccaccg tgcgcccgcc
     8101 gcgtgacgtg gtggccgggc gggcggcagc aggcgtacgc ggccggcgtg ccggcgagga
     8161 cgaggtcggc cgccgtgagg gccgcatcga ccgccgcccg cgcggcctcc caggtgcccg
     8221 gcccgacgag cgtcatcgtg tcgtacgcga agtagcccgg ccgcgcccac gtcgcggccg
     8281 gaacggcggg cgtctcggtc aggccggggt gcgcgaagac gtacgggacg acgcggtcct
     8341 ggcccgggtc gtccggcagg ccggccgcct cccagtccgc ccacgcgcgc gcgaggtagt
     8401 cgaggaggtc ggcgtcgtgc acggcgagga gccgctcgtc gggatgcggg tcggcgccga
     8461 cgatccgggc gccgtcggcc gcgagcgcgt cccggatcgc ctcggcgcgc gcggggacct
     8521 cggcggcggg catccgcacg ccgacccaga cctcgccgcc cggatcgtgc aggcggtggc
     8581 ggtcgctcca gacgacgggc aggccgagcg gcatcgcccg agcataagct ccgcgcgatg
     8641 tcgaccctgc gcgagcacgg gatccggatc ggcgagctcg agccgggacc ggccaacgcg
     8701 atcacggacg tccccgggtt ccgcgtcggc cacgtgaccg tgcgtcgcga cgagcccgag
     8761 ccgccggagg gccgcggcgt ggcccggacc ggcgtgaccg tcctcgtgcc gccgcggctt
     8821 ccccttcccg ccggcgcggc ggtgctgaac ggcgcgggcg agctgacggg cttcctccag
     8881 gtctccgagt ggggcgtcgt cgagacgccc gtctacctga ccgggacgat ggccgtaggg
     8941 cgcgtcttcg acggggccgt cgccgccgcg gtcgcggccg acccggcggt cggcgtggaa
     9001 accgtcgtga tcccggtcgt cggcgagtgc gacgacagct tcctctccga ggcgcgcgtc
     9061 gtccaggtgg aggcagccga cgccggccgg gcgctggccg cgacggcgga cggcccggtc
     9121 gagcagggcg cggtcggcgc gggcaccggg atgatcgcct tcggctggaa gggagggatc
     9181 ggctcggcga gccgcgtcgt gccggggcgc ggcaccctcg gcgtgctcgt gctcgccaac
     9241 ttcggcacgc gcggccagct ccgcgtcgac ggcgtcgtcg tcggccgtgc gcttccgccc
     9301 gacccgcacg agcgcggccc ggcggggagc tgcatcgcgc tcgtcgccac cgacgcgccg
     9361 ctcgccgccc cgcagctcga gcgcctcgcc cgccgcgccg gcctcgggct cgcgcgcacc
     9421 ggctcggtcg cccaccacgg gagcggcgag atcttcctcg cgctctcgtc cggcgaaggc
     9481 gagacggccg accgcgatct cgacgcgctc ttcgccgcga ccgtcgagac gaccgaggag
     9541 gcggtcctga acgcgctctg gtcggccgcg acgacgaccg gtcgcgaggg ccgcgtcgtg
     9601 gaggcacttc cacacgacga gacggtggcg gcgctccgcg cagcgcgacg caattagact
     9661 gcgcccatgg cgcaacgcga agtcgtccag ctcgatcggg gccgcattgc ggagctgacc
     9721 gagcgcgagg agaagcggct caacgagcgc acgcaggcgt cgcgggcgct ctacgagcgc
     9781 ggccggacgt cgctcgcggg cggcgtgccg tcctcgtacc acctccgcga cccgtggccg
     9841 atctacctcg accgcgggca aggcccgcgc gtctgggacg tggacgggaa cgagatgctc
     9901 gacttccaca acggcttcgg ctcgatggtg cagggccacg cgcacccggc ggtcgccgcg
     9961 gccgtgcgcg agcggctcga caagggcacg cacttcgccg cgccgaccga ggacgggatc
    10021 gtcgtcgccg aggagctcgc ccgccgcttc cgcctgccga agtggcgcta cacgaactcc
    10081 ggctccgagt cgacgatgga cgcgatccgc atcgcgcgcg cc
//
LOCUS       DASTFC010000125         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_743816,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000125 DASTFC010000000
VERSION     DASTFC010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743816"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="panC"
                     /locus_tag="VFO88_03700"
     CDS             <1..724
                     /gene="panC"
                     /locus_tag="VFO88_03700"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9544657.1"
                     /translation="ALHAGHRALLAAARAECDTVVASIFVNPAQFGPNEDLDRYPRYE
                     ASDLAALEQAGVDHAFLPSAVEMYPPGFQTWVEVEELGRGLEGDARPGHFRGVATVCL
                     KLFAIVRPDRVYFGQKDAQQAEVVTRMVDDLALGIDVRVVSTVRDEDGLALSSRNAYL
                     SPEERRAALALPRALAAGAEAHRAGGDPAAAARAALAAEPRVAPDYVEVARWNGRAVL
                     VAAARVGSTRLIDNVVLEGGWE"
     gene            721..1593
                     /gene="panB"
                     /locus_tag="VFO88_03705"
     CDS             721..1593
                     /gene="panB"
                     /locus_tag="VFO88_03705"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9544658.1"
                     /translation="MSTTGDRPAKVSLPELAQLARDGRPIVMVTAYDYPSGRLADEAG
                     VDVVLVGDSAAMTVLGHDSTVPATLEEMILLARATVRGARRPLVVGDMPFGTFQVSDE
                     ETVRNAVRFVKEAGVDAVKVEGAGPTLGRVQALVGAGIAVMGHVGLTPQSATLLGGFR
                     AQGRTAEAARRLLADALALEAAGCFAVVLEAVPAPVAARVTEALSVPTLGIGSGAECD
                     GQVLVLHDILGLYPGRSPRFVRRYAEVGDTIRDALERFAADVRDGAFPEEEHTYSIPD
                     DELAEFEAGLERRA"
     gene            complement(1590..2255)
                     /locus_tag="VFO88_03710"
     CDS             complement(1590..2255)
                     /locus_tag="VFO88_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9544659.1"
                     /translation="METGAGHATAEAGLEREWWLRTLAVFASPVSTFAALRDPSGSRA
                     DAREEPVLLVVLLAGITSVLATPAVGDLLDSRTRSAASVAVIIFLAGALYGTATYWIG
                     GGFLRLGLRAAGSEGTYRQARHLLAYAAAPLALALLLLWPLRLAVYGGDLFREGGADE
                     NGVGRAFDAAEVGFFAWAAFLLVLGVKVVHDWPVVRSIGALALAALALLVFSLAFLIV
                     GGR"
     gene            2397..2978
                     /locus_tag="VFO88_03715"
     CDS             2397..2978
                     /locus_tag="VFO88_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544660.1"
                     /translation="MARRTRKLLLSLLVVGAVGSVAAWGVFAAFTATTTNSGNTITAG
                     TVAIGQHAGATTLYSASNQGPGATTVGCVRVTYTGSLASSVRLYASAAPSNGDAFNLT
                     VERGSGLSSPDNTMNCTGFSASSTAFNGTLDTFAATYAGGIDGKAGGAAWAQNDAVDY
                     RFTVEVVDDPTPNAHSASVSTGAHSFTWEARNN"
     gene            3044..3562
                     /locus_tag="VFO88_03720"
     CDS             3044..3562
                     /locus_tag="VFO88_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544661.1"
                     /translation="MHAARTHIEWTALLLGAALAALAVAGLQVQGGIDTPPAEVTIAA
                     APSEELALTPPGGTATSGTLRASEPADGLEQRTTVQNATGGPLLVGLRAEPATSELDR
                     VLLVRVNAGGELVFDGTLGGLRAGAPGRFELASHESAEVTVQAWIPSAADEELWRGRV
                     ENVRLIYVTKAD"
     gene            3657..4217
                     /locus_tag="VFO88_03725"
     CDS             3657..4217
                     /locus_tag="VFO88_03725"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HET9544662.1"
                     /translation="MLVIGVAGLLAGAGSYAVFTATTGNTGNSFASGTVAIQDNDANT
                     AMLALANAKPGDADTSCIRIEYTGSLDSTVRLYGSVSGSLASYLTLTVARGTDSSPSF
                     DSCANFTPDATNYIGAGNGVVYSGPLSSFPASYAAGIVDPTSGSPETWSQSEAHSYRF
                     VVTLQDDNAAQGLTGNASFTWEARNT"
     gene            4214..4810
                     /locus_tag="VFO88_03730"
     CDS             4214..4810
                     /locus_tag="VFO88_03730"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9544663.1"
                     /translation="MSAVPHPSPLSAAYAKAWRARTRRSALRRLARVVAFALVGFALA
                     TAAAATVPTLFGFQSFAVLSGSMEPAIGTGDVVVVRKIAPLDAKIGDVVTFRSPEDAG
                     RLVTHRAASIQASGETVTFVTKGDANTGVERWSIAAGGTIGRVEYRLPKLGYVTNRVG
                     SRFGRLAFLVLPALVLAVSELRRIWSAEPKGSGDARQE"
BASE COUNT          669 a         1824 c         1731 g          602 t
ORIGIN      
        1 cgcgctccac gcgggacacc gggcgctcct cgcggccgcg cgggcggaat gcgacaccgt
       61 cgtcgcgagc atcttcgtga accccgcgca gttcgggccg aacgaggatc tcgaccgcta
      121 cccgcgctac gaggcctccg acctcgccgc gctcgagcag gcgggcgtcg accacgcctt
      181 cctcccgtcc gccgtggaga tgtacccgcc gggcttccag acctgggtcg aggtcgagga
      241 gctcggccgc gggctcgagg gcgacgcgcg gcccgggcac ttccgcggcg tcgcgaccgt
      301 ctgtctgaag ctcttcgcga tcgtgcggcc cgaccgcgtc tacttcggcc agaaggacgc
      361 gcagcaggcg gaggtcgtca cgcggatggt cgacgacctc gcgctcggga tcgacgtccg
      421 tgtggtttcg accgtgcggg acgaggatgg gctcgcgctc tcctcccgga acgcgtacct
      481 ctcgccggag gagcgccgcg cggcgctcgc gctgccgcgc gccctcgcgg cgggcgccga
      541 ggcccaccgc gcgggcggcg acccggccgc ggccgcccgc gccgcgctcg ccgccgagcc
      601 gcgcgtcgcg cccgactacg tggaggtcgc ccgctggaac gggcgcgccg tcctcgtcgc
      661 cgccgcccgc gtgggctcga cgcggctgat cgacaacgtc gtcctcgaag gaggctggga
      721 atgagcacga ccggcgaccg tccggccaag gtgtcgctcc ccgagctcgc gcagctcgcg
      781 cgcgacgggc gcccgatcgt gatggtgacc gcgtacgact acccgagcgg gcggctcgcg
      841 gacgaggccg gggtcgacgt cgtcctcgtc ggcgactcgg cggccatgac cgtgctcggg
      901 cacgactcga ccgtccccgc gacgctcgag gagatgatcc ttctcgcgcg cgcgaccgtg
      961 cgcggcgcgc ggcggccgct cgtcgtcggg gacatgccct tcgggacgtt ccaggtctcg
     1021 gacgaggaga cggtccgaaa cgccgtccgc ttcgtgaagg aggccggcgt ggacgcggtc
     1081 aaggtcgagg gcgccggccc gacgctcggc cgcgtgcagg cgctcgtcgg cgccgggatc
     1141 gccgtcatgg gccacgtcgg cctgacaccg cagtcggcga cgctcctcgg cggcttccgg
     1201 gcgcaggggc ggacggccga ggcggcccgg cggctcctcg cggacgcgct cgcgctcgag
     1261 gcggccggct gcttcgccgt cgtcctcgag gcggtgcccg cgccggtcgc tgcccgcgtg
     1321 accgaggcgc tctccgtccc gacgctcggg atcggctccg gcgccgagtg cgacgggcag
     1381 gtgctcgtgc tccacgacat cctcggcctc taccccggcc gctcgccgag gttcgtccgc
     1441 cgctacgcgg aggtcgggga cacgatccgc gacgcgctcg agcggttcgc ggcggacgtc
     1501 cgcgacggcg cgttcccgga ggaggagcac acgtactcga tcccggacga cgagctcgcc
     1561 gagttcgagg cagggctcga gcgtagggcc tagcggccgc cgacgatcag gaaggcgagg
     1621 ctgaagacga gcagcgcgag cgccgcgagc gcgagcgcgc cgatcgagcg gacgaccggc
     1681 cagtcgtgca cgaccttcac gccgagcacg agcaggaacg ccgcccaggc gaagaacccc
     1741 acctcggccg cgtcgaacgc gcgcccgacg ccgttctcgt ccgcgccgcc ctcgcggaag
     1801 aggtcgccgc cgtacacggc gaggcggagc ggccacagga gcaggagcgc gagcgcgagc
     1861 ggcgccgcgg cgtacgcgag caggtggcgc gcctggcggt acgtgccctc gcttccggcc
     1921 gcgcggagcc cgagccgcag gaacccgccg ccgatccagt acgtcgccgt cccgtagagc
     1981 gcgccggcga ggaagatgat cacggcgacg ctcgccgcgc tccgcgtgcg gctgtccagc
     2041 aggtccccca ccgccggcgt cgcgaggacg ctcgtgatcc cggcgaggag gacgacgagg
     2101 agcaccggct cctcacgcgc gtccgcgcgg gagcccgagg ggtcacgaag agccgcgaac
     2161 gtcgagacgg ggctcgcgaa gaccgcgagg gtgcggagcc accactcccg ctcgagccct
     2221 gcctccgccg tcgcgtggcc cgccccggtc tccacgccgc tcatcgtatc cccgccctac
     2281 gcagaaccgc gcgtcggcga tcacccgttc agggggtcgc agcgtccccg aaacgcgccg
     2341 acacagcaat cgcaagggaa tttcaaagac tgcggctacc taaaggagat acggacatgg
     2401 cgaggcgaac gcgcaagctg cttctctcgc tcctcgtcgt gggagcggtc ggaagcgtcg
     2461 cggcctgggg ggtctttgca gccttcacgg ccacgaccac gaacagcggc aacacgatca
     2521 cggccgggac cgtggcgatc ggccagcacg ccggcgcgac gacgctctat tccgcgtcga
     2581 accagggccc cggcgccacg accgtcggct gtgtgcgggt cacgtacacg ggctcgctcg
     2641 cgtcgagcgt caggctctac gcgtcggcgg cgccctcgaa cggcgacgcc ttcaacctca
     2701 cggtcgagcg cggctcgggc ctctcgtccc ccgacaacac gatgaactgc accggcttct
     2761 cggcgtcctc cacggcgttc aacggcaccc tcgacacgtt cgcggcgacg tacgccgggg
     2821 gcatcgacgg caaggccggc ggcgcggcgt gggcgcagaa cgacgcggtc gactaccggt
     2881 tcacggtcga ggtggtcgac gacccgacgc cgaacgcgca cagcgcgagc gtgagcaccg
     2941 gcgcccactc cttcacctgg gaggcgcgca acaactagga cgagcgcccg agtcgaggag
     3001 agcgggcccc tccccgctct cctcacggaa cggagcgact ccaatgcatg cggccagaac
     3061 gcacatcgag tggacggccc tgctcctcgg agctgccctc gcggccctcg cggtcgctgg
     3121 gttgcaggtc cagggcggga tcgacacgcc gcccgccgag gtgacgatcg cggccgcgcc
     3181 gtccgaggag ctcgccctca cgccgcccgg cgggacggcg acctcgggga cgctccgcgc
     3241 ctcggagccg gccgacggcc tcgagcagcg gacgacggtg cagaacgcga cgggcggccc
     3301 gctgctcgtc gggctccgcg ccgagcccgc gacctcggag ctcgaccgcg tcctgctcgt
     3361 ccgggtgaac gccgggggcg agctcgtctt cgacgggacg ctcggcgggc tgcgcgccgg
     3421 ggctcccggc cggttcgagc ttgcgagcca cgagtccgcc gaggtcacgg tgcaggcctg
     3481 gatcccgtcg gcggccgacg aggagctctg gcggggacgc gtggagaacg tccggctcat
     3541 ctacgtgacg aaggccgact gacgtgttcg gatacaggct cgagatctcg cgcgcgccga
     3601 ggccggcggc ccgcccgcgc ccacggtgga gccggagccg gcgcacggtc ctgacgatgc
     3661 tcgtcatcgg cgtcgccggc ctcctcgccg gcgccgggag ctacgcggtc ttcaccgcga
     3721 cgaccgggaa caccgggaac tcgttcgcct ccggcaccgt ggcgatccag gacaacgacg
     3781 cgaacaccgc gatgctcgcg ctcgcgaacg cgaagcccgg cgacgcggac acgagctgca
     3841 tccgcatcga gtacacgggc tcgctcgact cgaccgtgcg gctgtacggc tccgtgagcg
     3901 gctcgctcgc gagctacctc acgctcaccg tcgcccgcgg caccgactcg agcccgtcgt
     3961 tcgactcctg cgcgaacttc accccggacg cgacgaacta catcggcgcc gggaacggcg
     4021 tcgtctacag cggcccgctc tcatccttcc ccgcctcgta cgcggccggg atcgtcgacc
     4081 cgacgtccgg cagccccgag acgtggagcc agagcgaggc gcactcgtac cggttcgtcg
     4141 tgacgctcca ggacgacaac gcggcgcagg gcctcacggg caacgcgtcg ttcacgtggg
     4201 aggcgcgcaa cacgtgagcg ccgtcccgca tccgagcccg ctctcggccg catacgcgaa
     4261 ggcgtggcgg gcgcgcacga ggcggtcggc gctccgccgc ctggcgcgcg tcgtcgcctt
     4321 cgcgctcgtc ggcttcgcgc tcgcgacggc cgcggcggcg actgtcccca ccctcttcgg
     4381 cttccagagc ttcgccgtcc tctccggcag catggagcct gcgatcggca ccggcgacgt
     4441 cgtggtcgtc cgcaagatcg cgccgctcga cgcgaagatc ggcgacgtgg tgaccttccg
     4501 cagcccggag gacgccggcc gtctcgtcac ccatcgcgcg gcctcgatcc aggcgtccgg
     4561 cgaaaccgtc accttcgtga cgaagggcga cgcgaacacc ggcgtcgagc gctggtcgat
     4621 cgccgcgggc gggacgatcg gcagggtcga gtaccgcctc cccaagctcg gctacgtcac
     4681 gaaccgcgtc ggcagccgct tcggccgcct cgccttcctc gtcctgccgg ccctcgtgct
     4741 cgccgtctcc gagcttcgga ggatctggag cgcggagccg aaaggatccg gcgatgcgcg
     4801 ccaggagtag cgtcacccgg cccgtc
//
LOCUS       DASTFC010000126         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_150414,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000126 DASTFC010000000
VERSION     DASTFC010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150414"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFO88_03735"
     CDS             complement(<1..222)
                     /locus_tag="VFO88_03735"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9544664.1"
                     /translation="MAATAPKHDVKDLALAPSGVARIDWADRNMPVLAAIRERFEREK
                     PLDGYRVSACLHVTSETANLARTLKAGGAD"
     gene            complement(412..816)
                     /locus_tag="VFO88_03740"
     CDS             complement(412..816)
                     /locus_tag="VFO88_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9544665.1"
                     /translation="MSDASPNLLDLDRFGFDVERVEKPWGHEILFARSETYCGKVIFV
                     RAGEQLSLQFHRVKDETIYVHSGRIELEIGDPGGPLDTEVVGPGRSFRLRPGVVHRWR
                     ALEDSVVLEASTPDLDDVVRLEDNYGRAEPGA"
     gene            complement(813..1490)
                     /locus_tag="VFO88_03745"
     CDS             complement(813..1490)
                     /locus_tag="VFO88_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET9544666.1"
                     /translation="MEALLLAGGKGERLGATVPKPLVQVAGRPLAAYAVERLAAAGVA
                     RVLVACAAGQEDAFEQALGGLGAEIVAVGEPEPLGRGGGLRLAAESRDEEGPVYALNG
                     DEVLDLDLAALLAAHEERGPAATLVVAPLRSPFGIVELGTGDAVERFREAPVLPHWVN
                     AGFYVLGEEALARLPEKGDHEHTTFPELAAEGRLLAYRHEGMWLTVNTPKDLRAAEEA
                     LATGVAR"
     gene            complement(1490..>2703)
                     /locus_tag="VFO88_03750"
     CDS             complement(1490..>2703)
                     /locus_tag="VFO88_03750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET9544667.1"
                     /translation="GRVPIYEPGLVELVERNRGRLRFTLSLQDVFADARIAFVCVGTP
                     ALYSGDADLAAVWHVLDELPEFEERAVLVMKSTVPVGTGEKVRHELDSRGLAHVGYVS
                     NPEFLAEGTAVRDFTHPDRVVVGSFAEADGDAVAALYEPIEAPILRTDIASAEMIKLA
                     SNAFLATRISFINEIANVCEMTGADVVDVARGMGLDHRLGPHFLRAGLGYGGSCFPKD
                     VSALKQLAGNSGYHFQLLNAVIEVNELQKRRVIGKLTKHLGELRGKTVALLGLAFKPD
                     TNDLREASSLVLASRLLAEGARVRAWDPVALEDARPLLRGAELVDSVEDALRDADAAV
                     LVTEWNELRALATPGNAALMRNPLLVDGRNMLDPGAARAAGFAYEGIGRASSVLRAVG
                     EADVPEPDLRA"
BASE COUNT          368 a          995 c          984 g          356 t
ORIGIN      
        1 gtcggcgccg ccggccttga gcgtgcgcgc gaggttcgcg gtctccgagg tgacgtgcag
       61 gcaggcggag acccggtagc cgtcgagcgg cttctcccgc tcgaagcgct cccggatcgc
      121 ggcgaggacc ggcatgttcc ggtcggccca gtcgatgcgg gcaacgccgc tcggcgcgag
      181 cgcgagatcc ttgacgtcgt gcttcggtgc cgtcgccgcc acgggcggga gtctactcac
      241 gagcgccggg ccatgcgacg gtcggcgggc gactttgtgc cgtcgcggtc gggcttccgt
      301 caccccccgg cgccgctccc ggagcggcac cgcggcgctt tgtgcctgtc ccgcacaagg
      361 ggactatgtg acagccgcgg ggacgcggcc cgtgcggccg cgtcgaacga gtcaggcgcc
      421 gggctcggcg cggccgtagt tgtcctcgag ccggacgacg tcgtcgaggt cgggcgtcga
      481 cgcctcgagg acgaccgagt cctcgagcgc cctccagcgg tggacgacgc ccgggcgcag
      541 ccggaacgag cgcccgggcc cgacgacctc cgtgtcgagc ggcccgccgg gatcgccgat
      601 ctcgagctcg atccgccccg agtggacgta gatcgtctcg tccttcacac ggtggaactg
      661 gaggctgagc tgctcgccgg cccgcacgaa gatcaccttg ccgcagtacg tctccgagcg
      721 ggcgaacagg atctcgtggc cccacggctt ctcgacccgc tcgacgtcga agccgaagcg
      781 gtcgagatcg aggaggttcg gcgaggcgtc gctcatcgcg cgaccccggt cgcgagcgcc
      841 tcctcggccg cgcgcaggtc cttcggcgtg ttcaccgtga gccacatgcc ctcgtgacgg
      901 tacgcgagga gccggccctc ggcggccagc tcggggaacg tcgtgtgctc gtggtcgccc
      961 ttctccggca ggcgcgcgag cgcctcctcc ccgagcacgt agaagcccgc gttcacccag
     1021 tgcggcagga cgggcgcctc acggaagcgc tccacggcgt cgccggtccc gagctcgacg
     1081 atcccgaacg gcgagcggag cggcgcgacg acgagcgtcg ccgccgggcc gcgctcctcg
     1141 tgggcggcaa gcagcgccgc gaggtcgaga tcgagcacct cgtcgccgtt cagcgcgtac
     1201 accgggccct cctcatcccg ggactcggcc gcgaggcgaa gccccccgcc gcgcccgagc
     1261 ggctcgggct cgccgacggc gacgatctcc gcgccgaggc cgccgagcgc ctgctcgaag
     1321 gcgtcctcct gcccggccgc gcacgcgacg aggacgcgcg cgacccctgc ggcggcgaga
     1381 cgctcgaccg cgtaggcggc gagcggccgg ccggcgacct gcacgagcgg cttcgggacg
     1441 gtggcgccga gtcgctcgcc cttgcctccc gcgaggagga gcgcctccat caggcccgga
     1501 ggtccggctc ggggacgtcg gcctcgccga cggcgcgaag gacggacgac gcccgcccga
     1561 tcccctcgta cgcgaaccct gccgcccgcg ccgcgcccgg atcgagcatg ttgcgcccgt
     1621 ccacgagcag cgggttgcgc atgagcgccg cgttcccggg cgtcgcgagc gcacgaagct
     1681 cgttccactc ggtcacgagc acggccgcgt ccgcgtcccg gagcgcgtcc tcgaccgagt
     1741 cgacgagctc ggcgccgcgg agcaacggcc gcgcgtcctc gagcgcgacg ggatcccagg
     1801 cccgcacgcg cgcgccctcg gcgaggagac gcgacgcgag cacgagcgag gacgcctcgc
     1861 gcaggtcgtt cgtatccggc ttgaacgcga ggccgagcag cgcgacggtc tttccgcgca
     1921 gctccccgag gtgcttcgtg agcttcccga tcacgcgccg cttctgcagc tcgttcacct
     1981 cgatcacggc gttcaggagc tggaagtggt agcccgagtt ccccgcgagc tgcttcagcg
     2041 cggagacgtc tttcgggaag cagctccccc cgtagccgag gccggcccgg aggaagtgcg
     2101 gcccgaggcg gtggtcgagg cccatccctc gcgcgacgtc aacgacgtcg gcgcctgtca
     2161 tctcgcagac gttcgcgatc tcgttgatga agctgatccg cgtggcgagg aaggcgttcg
     2221 acgcgagctt gatcatctcc gccgaggcga tgtccgtccg caggatcggc gcctcgatcg
     2281 gctcgtagag cgccgcgacg gcgtccccgt cggcctcggc gaacgagccg acgaccacgc
     2341 ggtccgggtg ggtgaagtcg cgcaccgcgg tcccctcggc gaggaactcg ggattcgaga
     2401 cgtacccgac gtgggcgagg ccgcgggagt cgagctcgtg gcgcaccttc tcgcccgtgc
     2461 cgaccggcac cgtgctcttc atgacgagga cggcacgctc ctcgaactcg ggcagctcgt
     2521 cgagcacgtg ccagaccgcc gcgaggtcgg cgtcgcccga gtagagcgcc ggcgtcccga
     2581 cacagacgaa cgcgatgcgc gcgtcggcga agacgtcctg gagggagagg gtgaagcgaa
     2641 gccggccgcg gttgcgttcg acgagctcga cgaggcccgg ctcgtagatc ggcacgcggc
     2701 ccg
//
LOCUS       DASTFC010000127        14225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1172254,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000127 DASTFC010000000
VERSION     DASTFC010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172254"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFO88_03755"
     CDS             complement(<1..407)
                     /locus_tag="VFO88_03755"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF014870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9544668.1"
                     /translation="MTVPASLDERFRDRAAADGLLDVAYDLTDSPVGPLLLAATERGV
                     CRISFEPEPERELDALARWFGARVLRSPKPVDPLRRELDAYFEGTRREFDLAVDLTPL
                     PAFQRSVLEELARVRYGETATYGGLAARVGNPRA"
     gene            complement(404..898)
                     /locus_tag="VFO88_03760"
     CDS             complement(404..898)
                     /locus_tag="VFO88_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9544669.1"
                     /translation="MAPPRETTARAEAVGAPPFERFYATHKEEVYRYLVRMLGRQLAE
                     DAFQETFLRALRAYGDLRHARHLRAWAFTIATRVALDERRRARPEPLEAPEIPVADSR
                     PAYAELAHLADELPRTERAAVVLRYGYDLDYADIAQALGSSEEAARQAASSGVRRLRA
                     KGAA"
     gene            946..1749
                     /locus_tag="VFO88_03765"
     CDS             946..1749
                     /locus_tag="VFO88_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9544670.1"
                     /translation="MRVGVVGHVEWIEFVAVERVPRAGEIVTARESWGEAAGGGGVAA
                     VRLAELADECTLFTALGDDAWGRGCVAQLERLGVRCEVAWRETAQRRGFCYLDDDGER
                     TITLLSDKLRPRRDEPLAWERIAELDAVYFTGGDAGALRAARSARVLVATSRELDTLR
                     EAAVELDALVGSDDDPDERYEPGDLDPPPRLVFTTEGPDGGRIEPGRRRWAAAGLPAT
                     RADSYGAGDTFAARLAYALGEGRSPENAAAFAAAGSAEQLTRRGAHGLR"
     gene            1816..2718
                     /locus_tag="VFO88_03770"
     CDS             1816..2718
                     /locus_tag="VFO88_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544671.1"
                     /translation="MVAIGAAALVDAIRPGRGAAPPAAPTTVPAATARPELPAALPFT
                     TDLSRHTAALHGADAVGLLVVADPDCRIELVPLPELERVRLDLCARRLVEAGGTVTLA
                     GTTARRSGPRALRPDGALTIVRGGALREIRVGDRDSRLLLSREDLERLPLRGATELVG
                     IAWATNRRLFAILRRPRAPRFAFAVLEDGRLVGSECCFASLGGLRASPRGTYADVRSE
                     HGVLVYRRDGARVPLAPSSAPPADAVAWSPDERLAAVARGGAVDVLALDGRTRTAVVA
                     RLPVDAADLRWVPSEDHLVLPDWG"
     gene            2804..3238
                     /gene="mraZ"
                     /locus_tag="VFO88_03775"
     CDS             2804..3238
                     /gene="mraZ"
                     /locus_tag="VFO88_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET9544672.1"
                     /translation="MLLGEHAHALDDKNRVTLPAKFRQEFSSGVFVARGIDPCLLVYP
                     PDGWNRFVEERMAGLSPFSREARQMSRHVFANAIETGLDKQGRIMLPTSLLEHANLER
                     EVVVAGVNDHLELWEPAAWRRELDDVGRSVDRVAEHLAATEI"
     gene            3204..4169
                     /gene="rsmH"
                     /locus_tag="VFO88_03780"
     CDS             3204..4169
                     /gene="rsmH"
                     /locus_tag="VFO88_03780"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9544673.1"
                     /translation="MLPSTSQRPRSEHVPVLADEVRELLAVRPGETVIDATFGAGGHA
                     RVLCEDLGGRGRFIAIDRDPEARARFDRFAKSCGVQARFLRGDFSVVLGQLAQNGLEA
                     DAILLDLGVSSMQIDRPERGFSYAADAPLDMRMDPSEPVTAAALVNRSPERELADIFR
                     RYGEERFARQIARAIVKRRAERPFERTADLVEVVRAAIPAPRRFGDGHPAKRVFQALR
                     IAVNGELDSLAAALPSALQVLRPGGRIAVISFHSLEDRIVKRFFQAEARGCTCPPDFP
                     ICVCGKEPSLRLLARKAIRPSPDEVATNVRSASARLRAAVKTGGE"
     gene            4337..4567
                     /locus_tag="VFO88_03785"
     CDS             4337..4567
                     /locus_tag="VFO88_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544674.1"
                     /translation="MLLAGIVAVNVAALRLNLESQELEQRRQDLQAENATAASKLSSL
                     GAASRVEMTARGRLGLVDAVGASYVKARRPKR"
     gene            4564..6255
                     /locus_tag="VFO88_03790"
     CDS             4564..6255
                     /locus_tag="VFO88_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9544675.1"
                     /translation="MSARVVNRRVRLLVAAFALCFAVVLVRAGWLQAVRAGDLDRLAA
                     SQHRETITIPARRGTILDRTGVELAIGERAITVYANPRQIEDPRAVAFEAGKALDIAP
                     AKLVPILADRSKGFVYVARKADASAAEELQEQGIVGLGFYPEERRTYPLRNVAAEVVG
                     YAGTENRGLAGLELALDGTLAGTDGEKTIVRDPLGNELNVVDESEAVDGRDVRLTIDN
                     TLQRHVERVLAETRERWNAKATTAVVLDPRTGGILAMGVEPGFDANRFPNVPRDRQRN
                     RAVTDTFEPGSTFKVVTVSAALETGMVTPRTRYTLPDTIEVADRVISDAVPRGTEEFT
                     VSEIISRSSNVGTITLALGLGRTKLAEWIEKFGFGRPTGIEYPGETEGIVVPPELWSG
                     STIGNVPIGHGIAVTPLQMAQVYGAIANDGVAMPPRLVESVAGEEPKPVEGSRIVSEE
                     TAAAVTRMLRGVVSAGSGVEAAVPGYRVAGKTGTAAKPDPIEGGYSDTRYVASFAGFA
                     PAENPRFVVLVTVDEPSGTIWGGTAAAPAFREIATFALQYLRVPPDVPAELPVAP"
     gene            6320..7540
                     /locus_tag="VFO88_03795"
     CDS             6320..7540
                     /locus_tag="VFO88_03795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9544676.1"
                     /translation="MDRAAHPFLAGLTPAGAAWEFAVVWATAVGAIAYGVPADVARDD
                     WIAFAVLVPLIAAVHLLSRERANHQGSQLSLAPMFAAVLLLPPLLAAAAIALAFLPEW
                     ARMRTAWYIVVFNVANFVGPALAARAVFDRIAGVPTEAETAGWTLGALGALATFLVLQ
                     YAVLAVMLRLARGVPLRRTVRGDCVLIDASLLSVGALAAALAGHHPSLVALLAAPLAL
                     MYRSLAIPSLVELSRIEPKTGLYNMRHLEAVLAQELRRADRFDRPVSLLMIDVDHLRA
                     INSALGHVAGDRALRLVAMSLRAQTRDYDVAARFGGDEFCVLLPETSLEGALVVAERI
                     RADVEASGKGAGLGLTVSIGVAQTDERTTAEELVTRADRAAYRAKSGGRNVVALPPSD
                     PVGEAERLLSEASG"
     gene            7627..8328
                     /locus_tag="VFO88_03800"
     CDS             7627..8328
                     /locus_tag="VFO88_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544677.1"
                     /translation="MKLIAALLFVALLAGGYFVRQKDGREMTLDACVSEASEGFAASL
                     PPEYAEEITIAKIQESMTPLCGELLERPDVASAPDEDMPRLVSETFRSHPETFRPLCN
                     AVVDADFALLGELARYVRKQDRARFRHELCRLGVEYIDERSQVDHGRFVTDHPDLFAP
                     MCGAGIHAGLAEDPVLRRRLTPEQMRSIGRRACKRALVTGVFDVSGPGGFQQPRIDQR
                     AFEELVFRAVAAETR"
     gene            8328..8519
                     /locus_tag="VFO88_03805"
     CDS             8328..8519
                     /locus_tag="VFO88_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544678.1"
                     /translation="MSGGRVVLAVVCVVWGGGIVLTSLVEGIASPLDGAYEAGRFGGY
                     AFGGALVLAGARTLLDLRR"
     gene            complement(8539..9045)
                     /locus_tag="VFO88_03810"
     CDS             complement(8539..9045)
                     /locus_tag="VFO88_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9544679.1"
                     /translation="MSTGRDHTGALEALDRILNRESEADEVLRRTVELLHDRFDHYSW
                     VGIYLVEGDDLVLGPWKGPEATEHVRIPIGEGICGAAAASGRTEIVDDVNADERYLAC
                     FVSTRSEIVVPIAYEGRVVGEIDIDSDVPAAFDEDDRVFLERVAGLVSQHCLVAWDTG
                     GIAWRDVS"
     gene            complement(9042..9512)
                     /locus_tag="VFO88_03815"
     CDS             complement(9042..9512)
                     /locus_tag="VFO88_03815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9544680.1"
                     /translation="MTFSFGHASGRRPPFKYSSFTRVGFSDTDAQGIVYYGRYLPYFD
                     YARVEYSRHLGVLGHELSGCEFVMRACEVVYEAPARFDDLLEVFVRTKRLGRTSLTCE
                     FAAYRVADDELMCTAEQVLVLVHPGEKRSETIPDDYREPIERFEGADLELAVSR"
     gene            9676..12753
                     /locus_tag="VFO88_03820"
     CDS             9676..12753
                     /locus_tag="VFO88_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9544681.1"
                     /translation="MTQATAAGPLPKPGPGQKLNFYSALVSTEQYRDLLSKGLDIAAA
                     DAQADAMRLQMVLIPSQVRDLRAQGITVKLIRNKAGRTAIQEAAAQKSSGYTVWRDYD
                     GPDGFAAEIRRTARANPDITQAVAIGTTGQGRTIWALKVTAGARGAHPGRPSVLYSSL
                     QHAREWIAGEINWRLAEHYIARWRANDRAIKSLLQTTELWFVPVANPDGYQYTFQSPA
                     TRLWRKTLRDNNDNGTIEVGDGVDPNRNFPEHWNYDNEGSSGVPSSDTYRGPEAASEP
                     ETQAMIELLQEERFKFQVNYHSFGRWLLYPEGWQTGTPTADDPIYYALSGNLDNPAIG
                     SHATGDAFHPGLSSDVLYVTNGETTDYAHAQVDTLAWTPELSEGSPGSGFVFPDNPAL
                     VAEEFERNLPFALDVAKSAKDPDDPVSHLGLETEPFQLTSPDTYKAGIPGVNFAFPVS
                     YGDPQMVRVLAKRDLGTVEVKYRVNGGPVQSAPTTEWDGGEKYGSDTDVYYRVLDGFV
                     SGTEPGDSVEVWFEAESARSDSFTYEAQKESGRRVLVMAAEDYTGTSPGGPRPGPEYL
                     DDYLEMLAANGIEADVYDVDANGRKAPDAIGILSHYDAVVWYTGRDSFVREPGSTGNE
                     ASRLGMDQLFEIREYLNEGGRVLYTGDLAGGGYTTALGFFGLYDPTAENAFCEHLPAG
                     TDPRRCLFTFGSPASDLVNDVIEYWFGAYLFNFAAGNSATGFFPVTGTDTPFGGMEWM
                     IEPGVGFDFANSFITTSGILPPDEYPQFESWPAAKFDRPGGPFAPHSGEGYAYSQIAD
                     VSYKRLTKTVNVPAGGGEMTFWTSYNTEPDWDFVFVEAHTGDDDWTTLPDANGHTSQS
                     TGSPCPQVTSGGWRTLHPWLDHYQTQVGTTGCSPTGTTGEWHATSGDSHGWQQWRIDL
                     DEYAGEQVELSITYATDWATQGLGVFVDDVVFPDGSTAGFEGDLSGFSVPGPPPGSAP
                     NANDWIATDAEGFPEAAVVATPDTLYFGHGLEGIDPDQRDEVMGEAMEYLLRP"
     gene            complement(12809..14161)
                     /locus_tag="VFO88_03825"
     CDS             complement(12809..14161)
                     /locus_tag="VFO88_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET9544682.1"
                     /translation="MALDPKVFKAYDVRGIHPTELDEDGAYRIGRAYAEVFEPRRIAV
                     GRDMRLSAPTMAEAVIRGTADAGVDVLDIGMVGTEMVYFAVGEHGLDGGIAVTASHNP
                     KEYTGMKIVRRGALPVGGDSGLLEIRDRAERDPGEPVPAAERGAVEEADVYPGFVERV
                     LSFVDLDAIRPLRVVIDAANGMAGAMLPPVLDRLPVEAVRCYFDPDGSFPNHEPNPLL
                     PENREFIVAKVREERAELGIAFDGDADRCFFVDDTGEFVPGDFVTALLAEVMLEKEPG
                     RPVIYDVRASWAVPDAIRRAGGEPLVNRVGHAFIKHRMREVDALFAGEVSGHYYFRDF
                     EQADSGTVPALLMLALLSKAGKPLSELLRPYRERYFLTGELNTPVSDVALKLQEVKER
                     FGPEGEVSHIDGLEVTAEDWHMNVRPSNTEPLLRLNLEALGRELMERKRDEVLEVIRS
                     "
BASE COUNT         1905 a         5226 c         5174 g         1920 t
ORIGIN      
        1 gcgcgcgggt tcccgacgcg tgccgcgagc ccgccgtacg tcgccgtctc gccgtagcgc
       61 acgcgggcga gctcctcgag cacgctccgc tggaaggcgg ggagcggcgt caggtcgacc
      121 gccaggtcga actcgcgccg cgtgccctcg aagtacgcgt cgagctcgcg ccgcagcggg
      181 tcgaccggct tcggcgagcg gagcacccgg gcgccgaacc agcgggcgag cgcgtcgagc
      241 tcccgctcgg gctccggctc gaacgagatc cggcagacgc cgcgctccgt cgccgcgagg
      301 agcagcggcc cgaccggcga gtccgtcagg tcgtacgcga cgtcgagcag cccgtcggcg
      361 gcggcccggt cgcggaagcg ctcgtcgagc gaggcgggga cggtcatgcg gctcccttcg
      421 cgcgcagccg ccggacgccc gaggacgcgg cctggcgcgc cgcctcttcc gacgagccga
      481 gcgcctgcgc gatgtcggcg tagtccaggt cgtagccgta ccggaggacg accgccgccc
      541 gctccgttcg cggcagctcg tcggcgagat gggcgagctc cgcgtacgcc ggccggctgt
      601 cggcgaccgg gatctccggc gcctcgagcg gctcggggcg cgcgcggcgc cgctcgtcga
      661 gggcgacgcg cgtcgcgatc gtgaacgccc acgcccgcag gtggcgcgcg tgccgcaggt
      721 cgccgtacgc gcggagcgcg cggaggaacg tctcctggaa cgcgtcctcg gccagctgtc
      781 gtccgagcat ccgcacgagg tagcggtaca cctcctcctt gtgggtcgcg tagaaccgct
      841 cgaagggcgg cgcgcccacg gcctcggccc gggcggtcgt ctctcgtggc ggcgccatca
      901 caggggtata acgcgcggcc gccccgccgt gtgagactgc cgcgcatgcg ggtcggagtc
      961 gtgggtcacg tggagtggat cgagttcgtc gccgtcgagc gcgtaccgcg tgccggcgag
     1021 atcgtcaccg cgcgcgagag ctggggcgag gcggccggtg gcggaggcgt ggcggccgtc
     1081 cggctcgcgg agctcgcgga cgagtgcacg ctcttcaccg cgctcggcga cgacgcgtgg
     1141 gggcggggct gcgtcgcgca gctcgagcgc ctcggcgtgc gctgcgaggt ggcctggcgc
     1201 gagacggcgc agcggcgtgg cttctgctac ctggacgacg acggcgagcg cacgatcacg
     1261 ctcctctcgg acaagcttcg cccacggcgc gacgagccgc tcgcctggga gcggatcgcg
     1321 gagctcgacg ccgtctactt caccggcggc gacgccggcg cgctccgggc cgcccgcagc
     1381 gcgcgcgtgc tcgtcgccac ctcgcgcgag ctcgacacgc tcagggaggc cgccgtcgag
     1441 ctggacgcgc ttgtcgggag cgacgacgac ccggacgagc gctacgagcc gggcgacctc
     1501 gacccgccgc cccggctcgt cttcacgacc gaggggcccg acggcgggcg gatcgagccg
     1561 ggccgccggc gctgggccgc cgccggcctc ccggccacgc gggccgacag ctacggcgcg
     1621 ggcgacacgt tcgccgcccg cctcgcgtac gccctcggcg agggccgctc ccccgagaac
     1681 gcggccgcct tcgccgccgc cggctccgcg gagcagctca cccgccgcgg cgcacacggg
     1741 ctgcgctagc gggcgcttcc gcgctaccgt cgggtcgtag cggtgcgacc gtcgacattg
     1801 cttctcgtcg ggctcgtggt cgcgatcggc gcggccgcgc tcgtcgacgc gatccggccg
     1861 gggcggggcg cggcgccgcc ggccgcgccg acgacggtcc ccgccgcgac ggcgcggccg
     1921 gagctgccgg cggctctgcc gttcacgacc gacctctcgc ggcacaccgc cgccctgcac
     1981 ggcgccgacg ccgtcgggct gctcgtcgtc gccgaccccg actgccggat cgagctcgtt
     2041 ccgctgccgg agctcgagcg cgtgcggctc gacctctgcg cccgccggct cgtcgaggcg
     2101 ggcggcaccg tcacgctcgc cggcacgacg gcccgccgga gcgggccgcg ggcgctccgg
     2161 ccggacggcg ccctcacgat cgtccgcggc ggtgcgctcc gcgagatccg ggtgggggac
     2221 cgcgactccc gcctgctcct ctcgcgcgag gacctcgagc ggctgccgct tcggggcgcg
     2281 acggagctcg tcgggatcgc gtgggcgacg aaccgccgcc tgttcgcgat cctccgccgc
     2341 ccgcgggcgc cgcgcttcgc cttcgcggtg ctcgaggacg ggcggctcgt cggctccgag
     2401 tgctgcttcg cgtcgctcgg cggcctgcgc gcgagcccgc gcgggacgta cgccgacgtg
     2461 cgctcggagc acggcgtcct cgtgtaccgc cgcgacggcg cgcgggtgcc gctcgcgcct
     2521 tcctcggcgc cgcccgcgga cgcggtcgcc tggtccccgg acgagcgcct cgccgccgtc
     2581 gcgcgcggcg gggcggtcga cgtcctcgcg ctggacgggc ggactcggac ggccgtcgtc
     2641 gcccggctcc ccgtcgacgc cgccgacctg cggtgggtgc cgtccgagga ccacctcgtc
     2701 ctcccggact gggggtgagc acgcgcgtgg tacggggatg ttgcgttgtg gagatgtgtg
     2761 gggtaaagtg gggcgaagtg gtacggcgaa gggatcccgc ccaatgcttc tcggtgaaca
     2821 tgcgcacgct ctcgacgaca agaaccgcgt cacgctgccc gcgaagttcc ggcaggagtt
     2881 ctcgagcggc gtcttcgtcg cgcgcggcat cgatccctgc ctgctcgtct atccgccgga
     2941 cggctggaac cgcttcgtcg aggagcggat ggcgggcctc agcccgttca gccgggaggc
     3001 gcgccagatg agccgccacg tcttcgcgaa cgcgatcgag accggcctcg acaagcaggg
     3061 ccgcatcatg ctgcccacct cgctgctcga gcacgcgaac ctcgagcgag aggtcgtcgt
     3121 ggccggggtc aacgaccacc tcgagctctg ggagcccgcc gcgtggcgcc gggagctcga
     3181 cgacgtggga aggagcgtcg accgtgttgc cgagcacctc gcagcgaccg agatctgagc
     3241 acgtccccgt cctcgccgac gaggtgcgcg agctcctcgc cgtgcggccg ggcgagacgg
     3301 tcatcgacgc gacgttcggg gccggggggc acgcgcgcgt gctctgcgag gacctcggcg
     3361 ggcgcggccg cttcatcgcg atcgaccgcg acccggaggc ccgcgcgcgc ttcgaccgct
     3421 tcgcgaagag ctgcggtgtg caggcgcgct tcctccgcgg cgacttctcc gtcgtcctcg
     3481 gccagctcgc gcagaacggg ctcgaggccg acgcgatcct cctcgacctc ggcgtctcga
     3541 gcatgcagat cgaccggccg gagcgcgggt tctcgtatgc cgccgacgcg ccgctcgaca
     3601 tgcggatgga tccctccgag cccgtcacgg cggcggcgct cgtcaaccgg tcgcccgagc
     3661 gcgagctcgc ggacatcttc cgccgctacg gcgaggagcg cttcgcgcgc cagatcgcgc
     3721 gcgcgatcgt gaagcggcgc gccgagcgtc cgttcgagcg gacggccgac ctcgtggagg
     3781 tcgtccgcgc cgcgatcccg gccccgcgcc gcttcggcga cgggcacccg gcgaagcgcg
     3841 tcttccaggc gctccgcatc gccgtgaacg gggagctcga ctcgctcgcc gccgcgctgc
     3901 cttcggcgct ccaggtgctg cgccccggcg ggcggatcgc cgtgatcagc ttccactcgc
     3961 tcgaggaccg gatcgtcaag cgcttcttcc aggccgaggc gcgcgggtgc acgtgcccgc
     4021 ccgactttcc catctgcgtc tgcggaaagg agccgtcgct gcgcctgctc gcccgcaagg
     4081 cgatccgccc ctcgccggac gaggtggcga ccaacgtccg ctcggcgtcg gcgcggctgc
     4141 gcgccgccgt gaagaccgga ggggagtagc cgtggcggcc gccgctccgg cacgcgcacg
     4201 cccgcggcgg gcggcgaccg cgcccgcgcc gagcccgcgg ccccgtcccc gcccgcagcg
     4261 ctcccccgcg cgccggccgc gccccgccgc gcgtccgcgc gtcgcaggcg gcgtcctctg
     4321 gatcgtcgtt ctcgccgtgc tcctcgccgg catcgtcgcc gtgaacgtcg ccgccctccg
     4381 cctgaacctc gagtcgcagg agctcgagca gcgccgccag gacctccagg cggagaacgc
     4441 gacggcggcc tcgaagctct cgagcctggg cgcggcgagc cgggtcgaga tgaccgcgcg
     4501 cggccggctc gggctcgtgg acgcggtggg agcgagctac gtgaaggcgc gccggccgaa
     4561 gcggtgagcg cgcgcgtcgt gaaccggcgc gtccggctgc tcgtcgccgc gttcgcgctc
     4621 tgcttcgccg tcgtgctcgt ccgggccggg tggctgcagg ccgtccgcgc cggcgacctc
     4681 gatcgcctgg cggcgagcca gcaccgggag acgatcacga tcccggctcg ccgcgggacg
     4741 atcctcgacc gcacgggcgt cgagctcgcg atcggcgagc gcgcgatcac cgtctacgcg
     4801 aacccgcgcc agatcgagga tccccgcgcg gtcgccttcg aggccggcaa ggcgctcgac
     4861 atcgcgccgg cgaagctcgt cccgatcctc gccgaccgct cgaagggatt cgtctacgtc
     4921 gcgcgcaagg ccgacgcgag cgcggccgag gagctccagg agcagggcat cgtcggcctc
     4981 ggcttctatc cggaggagcg gcgcacctat ccgctgcgca acgtcgcggc cgaggtggtc
     5041 ggctacgccg ggacggagaa ccgcggcctc gccgggctcg agctcgcgct cgacgggacg
     5101 ctcgcgggga cggacggcga gaagacgatc gtccgcgacc cgctcgggaa cgagctgaac
     5161 gtggtagacg agagcgaggc ggtcgacggg cgcgacgtcc ggctcacgat cgacaacacc
     5221 ctccagcgcc acgtcgagcg cgtcctcgcc gagacgcgcg agcgctggaa cgcgaaggcg
     5281 acgacggcgg tcgtcctcga tcccagaacg ggagggatcc tcgcgatggg cgtcgagccg
     5341 ggcttcgacg cgaaccgctt cccgaacgtg ccgcgcgacc ggcagcgcaa ccgcgccgtc
     5401 acggacacgt tcgagccggg ctcgacgttc aaggtcgtga ccgtctccgc cgcgctcgag
     5461 accggcatgg tcacgccgcg gacgcgctac acgctccccg acacgatcga ggtcgcggac
     5521 cgcgtgatca gcgacgcggt gccgcgcggc acggaggagt tcacggtgtc ggagatcatc
     5581 tcccgctcct cgaacgtcgg gacgatcacc ctggcgctcg ggctcggccg gacgaagctc
     5641 gccgaatgga tcgagaagtt cggcttcggc cggccgaccg ggatcgagta ccccggtgag
     5701 acggagggga tcgtcgtccc gcccgagctc tggtccggct ccacgatcgg gaacgtcccg
     5761 atcgggcacg ggatcgcggt gacgccgctc cagatggcgc aggtctacgg cgcgatcgcg
     5821 aacgacggcg tcgccatgcc gccgcggctc gtcgagagcg tcgccggcga ggagccgaag
     5881 ccggtcgagg ggagccggat cgtctccgag gagaccgccg ccgccgtcac ccggatgctg
     5941 cggggggtcg tcagcgccgg ctcgggcgtc gaggcggccg tgccgggcta ccgcgtcgcg
     6001 gggaagaccg ggaccgccgc gaagcccgat ccgatcgagg gcggctactc ggacacgcgt
     6061 tatgtcgcct cgttcgccgg gttcgccccg gccgagaacc cgcgcttcgt cgtcctcgtg
     6121 acggtcgacg agccgagcgg gacgatctgg ggcggcaccg cggccgcgcc cgcgttccgc
     6181 gagatcgcga cgttcgcgct ccagtacctc agggtcccgc cggacgtgcc ggccgagctc
     6241 ccggtcgcgc cgtgacgccg gctcccgtcc ctcggacggg ggatgtctcc gccgccgggc
     6301 gcgccgtaga tcgtgataga tggatcgagc ggcgcacccc ttcctcgccg ggctgacgcc
     6361 ggccggcgcc gcctgggagt tcgcggtcgt ctgggcgacg gccgtcggcg cgatcgcgta
     6421 cggcgtgccg gccgacgtgg cgcgggacga ctggatcgcg ttcgccgtcc tcgtcccgct
     6481 catcgcggcg gtgcacctgc tctcgcgcga gcgcgcgaac caccagggat cgcagctctc
     6541 gctagccccg atgttcgccg ccgtgctcct cctgccgccg ctcctcgccg ccgcggcgat
     6601 cgcgctcgcc ttcctgccgg agtgggcgcg catgcggact gcgtggtaca tcgtcgtctt
     6661 caacgtcgcg aacttcgtcg ggcccgcgct cgcggcccgc gccgtcttcg accggatcgc
     6721 gggcgtcccg acggaggccg agacggcggg ctggacgctc ggcgcgctcg gggcgctcgc
     6781 gacgttcctc gtgctccagt acgccgttct cgcggtcatg ctccgcctcg cgcgcggggt
     6841 gccgctccgg cggacggtgc gcggagactg cgtcctcatc gacgcgagcc tcctctcggt
     6901 cggcgcgctc gcggccgcgc tcgccgggca tcacccgagc ctcgtcgcgc tcctcgcggc
     6961 gccgctcgcg ctcatgtacc gctcgctcgc catcccgagc ctcgtcgagc tctcgcggat
     7021 cgagccgaag accggcctct acaacatgcg ccacctcgag gcggtgctcg cccaggagct
     7081 gcgacgcgcc gaccgcttcg accggcccgt cagcctgctc atgatcgacg tcgaccacct
     7141 gcgcgcgatc aactcggcgc tcggccacgt cgccggcgac cgggcgctcc gcctcgtcgc
     7201 gatgtcgctc cgggcgcaga cccgcgacta cgacgtcgcc gcgcgcttcg gcggggacga
     7261 gttctgcgtg ctccttccgg agacgagcct cgagggcgcg ctcgtcgtcg ccgaacggat
     7321 ccgcgccgac gtcgaggcgt cgggaaaggg cgccgggctc ggcctcaccg tctcgatcgg
     7381 cgtcgcccag acggacgaga ggacgaccgc cgaggagctc gtgacgcgcg ccgaccgcgc
     7441 cgcgtatcgg gcgaagtcgg gcggcaggaa cgtcgtcgcg ctgcctccgt ccgacccggt
     7501 gggcgaggcc gagcgcctgc tctccgaggc ctccggctag gcgacgtcgc gccgtccctc
     7561 gtccgagggt ctcagcgggc gcggcggatt ccgacgcaac ggacagaccc tgcccggccc
     7621 gccgtcgtga agctcatcgc cgcactcctc ttcgtagcgc tcctcgcggg cggctacttc
     7681 gttcggcaga aggacggacg cgagatgacg ctcgacgcgt gcgtgagcga ggcgtcggag
     7741 ggattcgcgg cgagtctccc cccggagtac gcggaggaga tcacgatcgc caagatccag
     7801 gagagcatga cgccgctctg cggggagctg ctcgagcgac ccgacgtcgc gtcggccccc
     7861 gacgaggaca tgccgcgtct cgtcagcgag accttccggt cgcacccgga gacgttccgc
     7921 ccgctctgca acgcggtcgt ggacgcggac ttcgcgctcc tcggcgagct cgcccgctac
     7981 gtccggaaac aggaccgcgc ccgcttccgc catgagctct gccggctcgg cgtcgagtac
     8041 atcgacgagc ggtcgcaggt cgaccacggc cggttcgtga cggatcaccc cgacctcttc
     8101 gccccgatgt gcggcgccgg gatccacgcc gggctcgcgg aggaccccgt cctgcggagg
     8161 cgcctcacgc cggagcagat gcgctcgatc ggccgtcgcg cgtgcaagcg cgcgctcgtg
     8221 acgggcgtct tcgacgtcag cggcccgggc ggcttccagc agccccggat cgaccagcgg
     8281 gcgttcgagg agctcgtctt ccgggccgtc gccgccgaga cgcggtgatg agcggcggcc
     8341 gcgtcgtgct cgcggtcgtc tgcgtcgtct ggggcggcgg gatcgtcctc acgagcctcg
     8401 tcgaaggcat cgcgtcgccg ctcgacggag cgtacgaggc gggccggttc ggcggctacg
     8461 ccttcggcgg agcgctcgtc ctcgcgggcg cccgcacgct ccttgacctc cgtcgttgaa
     8521 gcgcggtcgt gcggccgctc aggacacgtc gcgccaggcg atgccgccgg tgtcccacgc
     8581 cacgaggcaa tgctgggaga cgaggccggc gacgcgctcc aggaacacgc ggtcgtcctc
     8641 gtcgaacgcg gcggggacgt cggagtcgat gtcgatctcc ccgacgacgc ggccctcgta
     8701 ggcgatcggc acgacgatct cgctccgcgt cgagacgaag cacgcgaggt agcgctcgtc
     8761 cgcgttcacg tcgtccacga tctccgttcg cccgctcgcc gcggccgcgc cgcagatgcc
     8821 ctcgccgatc gggatccgga cgtgctcggt cgcctcgggg cccttccacg ggccgagcac
     8881 gagatcgtcg ccctcgacga ggtagatccc gacccaggag tagtggtcga accggtcgtg
     8941 gaggagctcg accgtccggc ggagcacctc gtccgcctcg ctctcgcggt tcaggatccg
     9001 gtcgagcgcc tcgagcgcgc ccgtgtggtc gcggcccgtg ctcaccggct caccgccagc
     9061 tcgaggtccg cgccctcgaa ccgctcgatc ggctcgcggt agtcgtccgg gatcgtctcg
     9121 gagcgcttct cgcccggatg gacgaggacg agcacctgct cggcggtgca catgagctcg
     9181 tcgtccgcga cccggtacgc ggcgaactcg cacgtgaggc tcgtccggcc gagccgcttc
     9241 gtccggacga agacctcgag caggtcgtcg aagcgggcgg gcgcctcgta cacgacctcg
     9301 cacgcgcgca tgacgaactc gcacccggag agctcgtggc cgagcacccc gagatggcgc
     9361 gagtactcga cgcgcgcgta gtcgaagtag ggcaggtagc ggccgtagta gacgattccc
     9421 tgcgcgtccg tgtccgagaa ccccacgcgc gtgaaggacg agtacttgaa gggcggccgg
     9481 cgcccgctcg cgtgcccgaa ggagaacgtc acgcggccga tgctaacggt cgccggttcg
     9541 gacttgaatc gctgcaggat cggagtactc ttgcctgcct gcacctgtag tcgaacgaag
     9601 gggtagggga gcatgagacg atcgacgagg gctgcggccc ttgtcgtcgt gtcctgtgtg
     9661 gtcgccctcg gggcggtgac acaggccacg gcggcgggac cgctgccgaa gccggggccc
     9721 gggcagaagc tgaacttcta ctcggcgctc gtgtccacgg agcagtaccg cgacctgctg
     9781 tccaaggggc tcgacatcgc cgccgcggac gcccaggccg acgcgatgcg gctccagatg
     9841 gtgctgatcc cgtcgcaggt ccgggacctc cgcgcgcagg ggatcacggt caagctcatt
     9901 cgcaacaagg ccggcaggac ggcgatccag gaggccgccg cgcagaagtc gagcggctac
     9961 accgtctggc gcgactacga cggcccggac ggcttcgcgg ccgagatccg gcgcacggcc
    10021 cgcgcgaacc cggacatcac ccaggcggtg gcgatcggga cgaccggcca gggccggacg
    10081 atctgggcgc tcaaggtgac ggcgggcgcg cgtggtgccc accccgggcg tccgagcgtg
    10141 ctctacagct cgctccagca cgcccgcgag tggatcgcgg gcgagatcaa ctggcgcctg
    10201 gccgagcact acatcgcgcg gtggcgggcg aacgacaggg cgatcaagag cctcctgcag
    10261 acgacggagc tctggttcgt cccggtcgcg aacccggacg gctaccagta caccttccag
    10321 agcccggcga cgcggctctg gcgcaagacg ctccgtgaca acaacgacaa cgggacgatc
    10381 gaggtcggcg acggcgtcga cccgaaccgg aacttcccgg agcactggaa ctacgacaac
    10441 gagggctcgt cgggcgtccc gtcgagcgac acgtaccgcg ggccggaggc ggcctcggag
    10501 ccggagacgc aggcgatgat cgagctcctc caggaggagc ggttcaagtt ccaggtgaac
    10561 taccactcgt tcggccgctg gctgctctac cccgaggggt ggcagaccgg cacgccgacg
    10621 gcggacgacc cgatctacta cgccctctcg ggcaacctcg acaacccggc gatcgggagc
    10681 cacgcgaccg gtgacgcgtt ccatccgggc ctctcgtccg acgtcctcta cgtgacgaac
    10741 ggcgagacga ccgactacgc gcacgcgcag gtcgacacgc tcgcctggac gccggagctc
    10801 tccgagggct cgcccggctc gggcttcgtc ttcccggaca accccgcgct cgtcgccgag
    10861 gagttcgagc ggaacctccc gttcgcgctc gacgtggcga agtcggcgaa ggacccggac
    10921 gacccggtgt cccacctcgg gctcgagacg gagccgttcc agctcacgtc gcccgacacg
    10981 tacaaggctg ggatcccggg cgtgaacttc gccttcccgg tctcgtacgg cgacccgcag
    11041 atggtccgcg tgctcgcgaa gcgcgacctc ggcacggtcg aggtgaagta ccgcgtgaac
    11101 ggcggccccg tgcagtccgc ccccacgacc gagtgggacg gcggcgagaa gtacgggagc
    11161 gacaccgacg tctactaccg ggtcctcgac gggttcgtct ccggcacgga gccgggcgac
    11221 tcggtcgagg tctggttcga ggcggagagc gcccggagcg actccttcac gtacgaggcg
    11281 cagaaggaga gcggccggcg cgtgctcgtc atggccgccg aggactacac gggcacctcg
    11341 cccggcggcc cgcggcccgg cccagagtac ctcgacgact acctcgagat gctcgcggcg
    11401 aacgggatcg aggcggacgt ctacgacgtg gacgcgaacg gccgcaaggc gcccgacgcg
    11461 atcgggatcc tgtcccacta cgacgcggtc gtctggtaca cgggccgcga cagcttcgtc
    11521 cgcgagccgg gctcgaccgg caacgaggcc tcccgcctcg ggatggacca gctgttcgag
    11581 atccgcgagt acctgaacga gggcggccgc gtgctctaca cgggcgacct cgcgggcggc
    11641 ggctacacga cggcgctcgg cttcttcggg ctgtacgacc cgacggccga gaacgccttc
    11701 tgcgagcacc tcccggccgg caccgacccg aggcgctgcc tcttcacgtt cggctcgccg
    11761 gcgtcggatc tcgtgaacga cgtgatcgag tactggttcg gcgcgtacct cttcaacttc
    11821 gcggcgggca actcggccac cgggttcttc ccggtcaccg ggacggacac cccgttcggc
    11881 gggatggagt ggatgatcga gccgggcgtc ggcttcgact tcgccaactc gttcatcacg
    11941 acgagcggga tcctcccgcc ggacgagtac ccgcagttcg agagctggcc ggcggcgaag
    12001 ttcgaccgtc cgggcgggcc gttcgccccg cactcgggcg agggctacgc gtacagccag
    12061 atcgcggacg tctcgtacaa gcgtctgacg aagacggtga acgtcccggc cggcggcggc
    12121 gagatgacgt tctggacgtc gtacaacacc gagcccgact gggacttcgt cttcgtcgag
    12181 gcgcacacgg gtgacgacga ctggacgacg ctcccggacg cgaacggcca cacgtcgcag
    12241 agcacgggct cgccgtgccc ccaggtcacc tcgggcggct ggcggacgct ccacccgtgg
    12301 ctcgaccact accagacgca ggtcggcacg acgggctgct cgccgaccgg cacgaccggc
    12361 gagtggcacg cgacgtcggg cgactcgcac ggctggcagc agtggcggat cgacctcgac
    12421 gagtacgcgg gtgagcaggt cgagctctcg atcacgtacg ccaccgactg ggcgacgcag
    12481 ggcctcggcg tgttcgtcga cgacgtcgtc ttcccggacg gctcgacggc gggcttcgag
    12541 ggcgatctgt cgggcttctc cgtgcccggc ccgcccccgg gcagcgcgcc gaacgcgaac
    12601 gactggatcg ccaccgacgc cgagggcttc cccgaggcgg ccgtcgtggc gacgccggac
    12661 accctgtact tcggccacgg tctcgagggc atcgacccgg accagcggga cgaggtcatg
    12721 ggcgaggcga tggagtacct cctccggccg taacggcgga ggaacggcgg gtgacggcgg
    12781 gcgggcccac gggcccgccc gccgtcgtct acgaccggat gacctcgagc acctcgtcgc
    12841 gcttgcgctc catgagctcg cggccgaggg cctcgaggtt caggcgcagg agcggctcgg
    12901 tgttcgacgg gcggacgttc atgtgccagt cctcggccgt cacctcgagc ccgtcgatgt
    12961 gggacacctc gccctccggg ccgaagcgct ccttcacctc ctggagcttc agcgcgacgt
    13021 ccgagacggg cgtgttcagc tcgccggtca ggaagtagcg ctcgcggtac gggcggagca
    13081 gctcggagag cggcttcccc gccttcgaga ggagcgcgag catgagcagc gccgggaccg
    13141 tgcccgagtc ggcctgctcg aagtcgcgga agtagtagtg gccggagacc tcgccggcga
    13201 agagcgcgtc cacctcgcgc atgcggtgct tgatgaaggc gtgtcccacg cggttcacga
    13261 gcggctcgcc gcccgcgcgg cggatcgcgt ccggcacggc ccagctcgca cggacgtcgt
    13321 agatcaccgg ccgccccggc tccttctcga gcatcacctc ggcgaggagc gccgtcacga
    13381 agtcgccggg gacgaactcg cccgtgtcgt cgacgaagaa gcagcggtcg gcgtcgccgt
    13441 cgaacgcgat tccgagctcg gcacgctctt cacgcacctt ggcgacgatg aactcgcggt
    13501 tctcgggcag gagcgggttc ggctcgtggt tcgggaagct tccgtccgga tcgaagtagc
    13561 aacggacggc ctcgaccggc aggcggtcga ggacgggcgg gagcatcgcg ccggccatgc
    13621 cgttcgcggc gtcgatgacg acgcgcagcg gtcggatcgc gtcgaggtcg acgaacgaga
    13681 ggacgcgctc gacgaatccg gggtagacgt ccgcctcctc cacggcgccg cgctcggcgg
    13741 ccggcacggg ctcgccgggg tcgcgctcgg cgcggtcgcg gatctccagg agcccggagt
    13801 cgccgccgac ggggagcgct ccgcggcgca cgatcttcat ccccgtgtac tccttcgggt
    13861 tgtgcgaggc ggtgaccgcg atgccgccgt cgagcccgtg ctcgccgacc gcgaagtaga
    13921 ccatctcggt accgaccatc ccgatgtcga ggacgtcgac gccggcgtcc gcggttccgc
    13981 ggattaccgc ctccgccatc gtcggcgcgg agagtcgcat gtcgcggccg accgcgatcc
    14041 ggcggggctc gaagacctcc gcgtaagcgc ggccgatccg gtacgccccg tcctcgtcga
    14101 gctccgtcgg gtggatccca cggacgtcgt atgccttgaa gaccttcggg tcgagcgcca
    14161 tggcgcggtc atcctaggcg gagtccggca cgaagccgtc acacatccgg cacgaagcgc
    14221 gcgag
//
LOCUS       DASTFC010000128         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_661414,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000128 DASTFC010000000
VERSION     DASTFC010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661414"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..999)
                     /locus_tag="VFO88_03830"
     CDS             complement(13..999)
                     /locus_tag="VFO88_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET9544683.1"
                     /translation="MLAPTARLLELLELLQAQTLTTGREISDRLGIDRRTVRRYVTAL
                     QELGIPVEGQRGVGGGYRIRPGFRLPPLMLDDDEAVVLALGAVAAGRLGLQGSEEAVD
                     GALAKLHRVLPDALRRRVEALEATLDFTAAAPDGAPVAGDTALLVAEAIRRRRRLRTR
                     YRSYAGEDTRRELSPYGLVVHSGRWYLAAHDHGREALRTFRVDRMRELGLLGEPAVPP
                     PDGFDAVAHVSRSLARVPWRWEVEVLLHLPPEEAARRLPATLAELVEAGEGTLLRMRV
                     GSLDWTAGLLAGLDCGFTIRSPEELRASLRALARRLEAQADAEMHPAAPPGP"
     gene            1082..1579
                     /locus_tag="VFO88_03835"
     CDS             1082..1579
                     /locus_tag="VFO88_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544684.1"
                     /translation="MGETAQAESTARDFDFWMGRWKVRNTFLRERLAGSDEWDEFEAT
                     SVARPLLDGLGNEDEFRTGYGGGFVAMSFRFFDPESRLWAIYWADSRRPGRLDPPVFG
                     SFSGDTGVFEGTDTFDGRPIVVRFVWSRVTTPTPRWEQAFSADGGDTWETNWVMEFTR
                     AEDGR"
     gene            1576..2076
                     /locus_tag="VFO88_03840"
     CDS             1576..2076
                     /locus_tag="VFO88_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544685.1"
                     /translation="MSVLEQLGAVRPDYHHVSKLVRPAESLMLDDALLKWYDVAPADA
                     RVPSGVRALARRNLRAGVRSGALELSGDLGFVVLHRCGDDFYFLLVCTWRNDNELWET
                     VWAKRGDDDEFTPWPVEGTHRPTFCVWELGAVCHERKAWTGYLRSARDEAAKLAYARD
                     TFEGEA"
     gene            complement(2110..2511)
                     /locus_tag="VFO88_03845"
     CDS             complement(2110..2511)
                     /locus_tag="VFO88_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9544686.1"
                     /translation="MATHAASLTRDQLAALPDSYFRAVDAKDVDAILDHFADDATLTV
                     QTDHAVFSGAEEIRRMFTDFCGGSVSIFHEIKNIVVEPDAGKVATEQSYIGELVDGTK
                     NDMHNCNFFDVGPDGRFTRVIIWMAGQNPLK"
     gene            2603..>3191
                     /locus_tag="VFO88_03850"
     CDS             2603..>3191
                     /locus_tag="VFO88_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544687.1"
                     /translation="MAIRRYLPWLRPVGVLAGLALFIGGTVALFTIENSTGSLFVVTI
                     GMVLALVALLGDRVQLESFDILGANVKVREVVQGRLRLAESPDAANADPRSSLAREQA
                     STLQKLAGLYDLYRYVRRTQPASDERTAALDELARRMQAAGSEAEFDPAEVATWFHEG
                     DDALRVIALNVMLARAECRQLFAVLETIEEPRSLFE"
BASE COUNT          433 a         1104 c         1186 g          468 t
ORIGIN      
        1 cggctgatcc ggtcagggcc cgggaggcgc ggccggatgc atctcggcat ccgcctgcgc
       61 ctccagcctt cgggcgagcg cccgcaggct cgcgcgaagc tcctcgggac tgcggatcgt
      121 gaacccgcag tcgagccccg cgagcaggcc ggccgtccag tcgagcgagc ccacgcgcat
      181 gcgcaggagc gttccctcgc cggcttccac gagctcggcg agggtggcgg gaagccgccg
      241 cgcagcctct tcgggcggaa ggtgcaggag cacctcgacc tcccaccgcc agggcacgcg
      301 cgcgagggag cggctgacgt gcgcgacggc gtcgaagccg tccgggggcg ggacggccgg
      361 ctcgccgagg aggccgagct cgcgcatgcg gtcgacgcgg aacgtccgca gggcttcgcg
      421 gccgtggtcg tgcgcggcga ggtaccagcg cccggagtgg acgacgaggc cgtacgggct
      481 cagctcgcgc cgcgtgtcct cgccggcgta cgaccggtac ctcgtccgga ggcgccggcg
      541 ccggcggatc gcctcggcga cgagcagggc ggtgtcgccg gcgaccggtg ctccgtcggg
      601 tgcggccgcg gtgaagtcga gcgtcgcttc gagcgcctcc acccgccggc gcagggcgtc
      661 cggcagcacc cggtggagct tcgccagggc gccgtccacg gcctcctcgg agccctgcag
      721 ccccaggcgg cctgcggcga ccgcgccgag cgcgaggacg acggcctcgt cgtcgtcgag
      781 catgagcggg ggcagacgaa agcccggccg gatccggtag ccgccgccga cgccgcgctg
      841 gccctccacc gggatcccca gctcctgcag tgccgtgaca tagcgccgga ccgtgcggcg
      901 gtcgatcccg agccggtcgg agatctccct ccccgtcgtg agggtctgtg cctgcaggag
      961 ctcgagcagc tcgagcagcc gtgcggtcgg cgcgagcacg cgctgagtga agcacagata
     1021 gcgggcacgg atcgccctat ttcctcgcta gcgtcacgtt cgagcgacga aggagggcga
     1081 gatgggcgag acggcgcagg cggagagcac ggcgcgagac ttcgacttct ggatgggccg
     1141 gtggaaggtg cgaaacacct tcctccgcga gcggctcgcg ggctcggacg agtgggacga
     1201 gttcgaggcg acgagcgtcg cccgccccct gcttgacggg ctcggcaacg aggacgagtt
     1261 ccgcacgggc tacgggggcg gcttcgtcgc catgtccttc cgcttcttcg atcccgagtc
     1321 gaggctgtgg gcgatctact gggccgacag ccgccggccc ggccggctcg acccaccggt
     1381 cttcggctcg ttctccggag acacgggcgt cttcgagggc acggacacgt tcgacggccg
     1441 gccgatcgtc gtgcgcttcg tctggtcgcg cgtgacgacg ccgacgccgc gctgggagca
     1501 ggcgttctcg gccgacggcg gcgacacgtg ggagacgaac tgggtgatgg agttcacgcg
     1561 ggcggaggac gggcgatgag cgtgctcgag cagctcggcg ccgtgcggcc cgactaccac
     1621 cacgtctcca agctcgtccg cccggccgag agcctcatgc tcgacgacgc gctcctcaag
     1681 tggtacgacg tcgccccggc cgacgcgcgg gtcccgagcg gcgtccgcgc gctggcgcgc
     1741 cgcaacctgc gcgccggcgt gaggtcggga gcgctcgagc tctcgggcga cctcgggttc
     1801 gtcgtcctgc accggtgcgg cgacgacttc tacttcctgc tcgtctgcac gtggcggaac
     1861 gacaacgagc tctgggagac ggtctgggcg aagcgcggcg acgacgacga gttcacgccc
     1921 tggccggtcg agggcacgca ccggccgacc ttttgcgtgt gggagctcgg cgcggtctgc
     1981 cacgagcgca aggcgtggac gggctacctg cgctcggcgc gcgacgaggc ggcgaagctc
     2041 gcgtatgcgc gggacacctt cgagggcgag gcctgacgga gcccttccgt tcccctcgac
     2101 gccgcggcgc tacttcagag ggttctggcc ggccatccag atgatgaccc gcgtgaacct
     2161 cccgtcgggg ccgacgtcga agaagttgca gttgtgcatg tcgttcttcg tgccgtcgac
     2221 gagctcgccg atgtagctct gctccgtcgc gaccttgccg gcgtccggct cgacgacgat
     2281 gttcttgatc tcgtggaaga tcgagacgga gccgccgcag aagtccgtga acatgcgccg
     2341 gatctcctct gcgcccgaga agaccgcgtg gtcggtctgg accgtgagcg tcgcgtcgtc
     2401 ggcgaagtgg tcgaggatcg cgtcgacgtc cttcgcgtcg accgccctga agtagctgtc
     2461 gggaagagcg gcgagctggt ccctcgtgag ggatgcggcg tgcgtggcca tccgtgcctc
     2521 cttcgtcgat gccgggcctc gatcctatcc cgaccggcca cgcggtcggg gcgcccgcac
     2581 gctcgggcag aatctcccct ccgtggcgat tcggagatac ctcccctggc ttcggccggt
     2641 cggggtgctg gccggcctcg cgctcttcat cggcgggacg gtcgcgctgt tcacgatcga
     2701 gaacagcaca ggcagcctgt tcgtcgtcac gatcgggatg gtgctggcgc tcgtcgcgct
     2761 gctcggcgat cgcgtgcagc tcgagtcgtt cgacatcctg ggggcgaacg tcaaggtgcg
     2821 ggaggtggtg cagggtcgtc ttcgcctcgc cgagtccccg gacgcggcga acgccgaccc
     2881 ccggagctcg ctcgcgcgcg agcaggcgtc gaccctccag aagctggccg gcctgtacga
     2941 cctgtaccgg tacgtccgcc ggacgcagcc ggcctcggac gagcgaaccg ccgcgctcga
     3001 cgagctcgca cgtcggatgc aggccgccgg cagcgaggcg gagttcgacc cggcagaggt
     3061 ggccacgtgg ttccacgagg gagacgacgc gctccgggtc atcgcgttga acgtgatgct
     3121 cgcgcgcgcg gagtgccggc agctcttcgc cgttctcgag acgatcgagg agccgcgcag
     3181 cctcttcgag c
//
LOCUS       DASTFC010000129         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_677952,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000129 DASTFC010000000
VERSION     DASTFC010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677952"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..277
                     /locus_tag="VFO88_03855"
     CDS             50..277
                     /locus_tag="VFO88_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544688.1"
                     /translation="MGADRKHLDDRQLDALLERLDRLCDEVHGLNRRLDQNDALLRIG
                     GELANLNESLQALAYAALGQQGPQVRRRRSA"
     gene            complement(288..1502)
                     /locus_tag="VFO88_03860"
     CDS             complement(288..1502)
                     /locus_tag="VFO88_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544689.1"
                     /translation="MPRPRSRRGLLLAALAVALIAAGAAAGAVLLTRDDGGDAGAGGE
                     AMPGATDAHPVAGTFEPDDLSLADCEGSDSRCIEQAFGNLAYAEGPQRAIADFDAAMQ
                     ADPSVESNCHRIAHTIGSAALARFEGDVGEAFAAGSASCWSGYYHGILERAFADLESL
                     DQAGPVARELCKGERIEETQFLLYQCVHGLGHGLMIHSGYDLPWSLGICDELADQWSQ
                     SSCTGGVFMENIASSYDVRSEWIRDDDPVYPCEDVADRHKYYCYLMVTSRVNELNGFD
                     WADTASICAGVEADWVAICFESYGRDASGSTRQDAAEIVRICGLAGRPAYEESCLYGA
                     ARDITSNDAGGERAAVLCARADARHRSRCYAGIGTIVANLFPTEEEQRGACVSTVPRR
                     FLADCLSGAGLA"
     gene            1797..>2779
                     /locus_tag="VFO88_03865"
     CDS             1797..>2779
                     /locus_tag="VFO88_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET9544690.1"
                     /translation="MRRRSLHPAQLVVLAFATAILVGTLLLLLPVARVGDEGASFLTA
                     LFTATSAVCVTGLVVVDTETYWSPTGQVVIMALIQVGGFGIMALTSLLALLVARRLGL
                     RTRLLAQTETKALQLGDVRRVLLGVAILSLLFETATMLVLTGRLWLAYDYGLGDAAWH
                     GAFHAVSAFNNAGFSLWSDNLMGFVTDGWISLAVAGAVICGGLGFPVWIELARRGLTW
                     GRWSIHTKLTLAVTAVLLVAGTVAVLAFEWSQPETLGPLGVGGKLLAGFFQGVMPRTA
                     GFNSLDYGELQPETLLLTEILMFVGGGSASTAGGIKVTTLALLALMVVAEVR"
BASE COUNT          369 a         1033 c          975 g          402 t
ORIGIN      
        1 tcgtccactc ggccgacgac ctcgccgccc acggctacga tcaggggtga tgggtgcgga
       61 tcggaagcat ctggacgacc ggcagctgga cgcgctgctc gagcggctcg accggctctg
      121 cgacgaggtg cacgggctga accgccggct cgaccagaac gacgcgctcc tccggatcgg
      181 cggcgagctc gcgaacctca acgagtcgct ccaggccctc gcgtacgccg cgctcggcca
      241 gcagggcccg caggtccgcc ggcgccggtc cgcctaggag gccggcacta cgcgagcccg
      301 gcgccgctca ggcagtcggc gaggaaccgg cgcggcaccg tggagacgca cgcgccccgc
      361 tgctcctcct ccgtcgggaa gaggttcgcg acgatcgtcc cgatcccggc gtagcagcgg
      421 gaccggtggc gcgcgtccgc ccgggcgcag agcaccgcgg cccgctcgcc gcccgcgtcg
      481 ttcgacgtga tgtcccgcgc cgcgccgtag aggcagctct cctcgtacgc gggacgcccg
      541 gcgaggccgc agatccggac gatctccgcc gcgtcctgcc gcgtcgaccc ggacgcgtca
      601 cgtccgtacg actcgaagca gatcgcgacc cagtcggctt cgacgcccgc gcagatgctc
      661 gccgtgtcgg cccagtcgaa cccgttcagc tcgttcacgc gcgaggtgac catgaggtag
      721 cagtagtact tgtgccgatc ggccacgtcc tcgcacgggt agacggggtc gtcgtcgcgg
      781 atccactccg accgcacgtc gtagctcgac gcgatgttct ccatgaagac gccgccggtg
      841 caggaggact gcgaccactg gtcggcgagc tcgtcgcaga tcccgagcga ccagggcagg
      901 tcgtagcccg agtggatcat gagcccgtgc ccgagcccgt ggacgcactg gtagaggagg
      961 aactgcgtct cctcgatccg ctcgcccttg cagagctccc gcgcgaccgg gcctgcctgg
     1021 tcgagcgact cgagatcggc gaaggcgcgc tcgaggatcc cgtggtagta gccggaccag
     1081 cacgacgccg agccggccgc gaacgcctcg ccgacgtcgc cctcgaagcg cgcgagcgcg
     1141 gccgagccga tcgtgtgcgc gattcggtgg cagttgctct cgacggaggg atccgcctgc
     1201 atcgcggcgt cgaagtcggc gatcgcgcgc tgggggccct cggcgtacgc gaggttcccg
     1261 aacgcctgct cgatgcagcg ggagtccgag ccctcgcagt cggcgaggga caggtcgtcc
     1321 ggctcgaacg tgcccgcgac cgggtgcgcg tccgtcgcgc ccggcatcgc ctcgccgccg
     1381 gcgcccgcgt cgccgccgtc gtcgcgcgtg aggagcaccg cgcccgcagc cgctcccgcc
     1441 gcgatcagcg cgacggcgag tgccgcgagc aggagcccgc gccggctacg aggtctcggc
     1501 atccctcaag agtacgccgc ggccgaccgg aaccgccggg gtcggtcggc gaacggtgag
     1561 caggccctag gagcggcagg cgggccagga gccccgccga tcgtcgaggc gtgagtccgc
     1621 gccgaagaac accgcgagcg ggccgacgag gaggaagaac gcgaggagga tgatctgtgc
     1681 catgcccctt ccatcggcat ccgcgcgggg cgccttgaaa aggacgtggc ccgttcgagg
     1741 cgaacccccg cccgtgcggc ccgacactcg tcaccgcgcc gcctgaggag cccggcgtgc
     1801 gtcgtcgctc cctccatccg gcccagctcg tcgtcctcgc cttcgccacg gcgatcctcg
     1861 tcgggacgct cctgctcctg ctcccggtcg cgcgggtcgg cgacgagggc gcctccttcc
     1921 tcaccgcgct cttcacggcg acgtcggcgg tctgcgtgac cgggctcgtg gtggtcgaca
     1981 cggagacgta ctggtcgccc accggccagg tcgtgatcat ggcgctcatc caggtcggcg
     2041 gcttcgggat catggcgctg acctcgcttc tcgcgctgct cgtcgcgcgc cgcctcggcc
     2101 tgcggacacg cctgctcgcc cagacggaga cgaaggcgct ccagctcggc gacgtccggc
     2161 gcgtgctcct cggggtcgcg atcctcagcc tcctcttcga gacggcgacg atgctcgtcc
     2221 tcaccggccg gctttggctc gcgtacgact acgggctcgg cgacgctgcc tggcacggcg
     2281 cgttccacgc cgtctccgcc ttcaacaacg cgggcttctc gctctggagc gacaacctca
     2341 tgggcttcgt gacggacggc tggatctccc tcgcggtcgc cggagccgtg atctgcggcg
     2401 gcctcgggtt cccggtctgg atcgagctcg ccaggcgcgg cctcacctgg ggacgctggt
     2461 cgatccacac gaagctgacc ctcgccgtga ccgccgtcct gctcgtcgcc ggcacggtcg
     2521 ccgtgctcgc gttcgagtgg tcgcagccgg agacgctcgg cccgctcggc gtcggcggga
     2581 agctgctcgc cgggttcttc cagggcgtca tgccgcgcac ggcgggcttc aactcgctcg
     2641 actacggcga gctccagccg gagacgctcc tcctgaccga gatcctcatg ttcgtcgggg
     2701 gcgggagcgc ctcgaccgcc ggcgggatca aggtgacgac gctcgcgctc ctcgcgctca
     2761 tggtcgtcgc ggaggttcg
//
LOCUS       DASTFC010000130         7000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_628779,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000130 DASTFC010000000
VERSION     DASTFC010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628779"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFO88_03870"
     CDS             complement(<1..311)
                     /locus_tag="VFO88_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET9544691.1"
                     /translation="MHTSDELREGFLSFFEGKGHLRRPSGSLVPATYDPSVLLTTAGM
                     QPLKPYFLGLEQPPAPLLTTVQKCFRTTDIDEVGLTARHLTFFEMLGNFSIGDYFKDG
                     AV"
     gene            complement(504..1280)
                     /locus_tag="VFO88_03875"
     CDS             complement(504..1280)
                     /locus_tag="VFO88_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HET9544692.1"
                     /translation="MSAPPEKNDRELLRRYHHQGDVEARQRLIEQYLPLVRSLARRYS
                     YRGEQLEDLVQVGCIGLIKAIDRFDVDRGVELTTYATPNIIGEIKRHFRDKGWAVRVP
                     RGLQELNVRLSRLMEELTVQYERSPTIAELAKAADVEEEEVLEALETGQAYATLSLSA
                     PSGGGDDGDDLDPLESLGEIEKEYEVSEDRALLAPGWKVLDDRERRILELRFFEGLTQ
                     SQIAQQVGISQMHVSRLIRRSLEKIREEIAVDASDLHPNA"
     gene            complement(1277..1675)
                     /locus_tag="VFO88_03880"
     CDS             complement(1277..1675)
                     /locus_tag="VFO88_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544693.1"
                     /translation="MREAITLTVPFERPYQGVVRLVLGGLAARLDLPYETLEDVQLAV
                     ETILGNDAYAVGDEVSVEVQLDGSSLAVAVGPLEPGALEHDLQASDDVQGVALGRLLA
                     ATMGEHELEARDGQGWLRMRKPIPAGEPTA"
     gene            complement(1764..3050)
                     /gene="mnmA"
                     /locus_tag="VFO88_03885"
     CDS             complement(1764..3050)
                     /gene="mnmA"
                     /locus_tag="VFO88_03885"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET9544694.1"
                     /translation="MVEAVGNAVRGDDLAWIRLTVDRGRIVDAQGDGPGVAALSREIR
                     GRDALEAAAVPAAPLAADALAEALAPVAAARPPRDRARVAVAMSGGVDSAVALLRVRE
                     AGAEPVGVTLRLWTDPAGPDGERVCCSPSAVVAARETCQGLGIPHFTLDLREEFRRAV
                     VGPFVAGYARGETPNPCIRCNGSFRFAELLAFARRIGAERLATGHYARLREHRGRLLL
                     ARGADAEKDQSYMLARLDLRHLGRVSFPLGDWTKGETRAYAAAHGLPAAGRAESQEAC
                     FLAGGDYRDFLERHGLATADGPVTDESGRELGRHAGYWRFTPGQRKGLGVAGGRPLYA
                     LRADPRTNTVVVGPRERLGRQAIGVRNGRLFVPVARAEVKLRYRSPGAPARVEPRARG
                     FRLRLDSPAYGVAPGQAAVLYEADAVVGAGLIASAS"
     gene            complement(3290..3715)
                     /locus_tag="VFO88_03890"
     CDS             complement(3290..3715)
                     /locus_tag="VFO88_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544695.1"
                     /translation="MRGQLLERLLALAAVALLATLVSLALVRDGDGEDGGSASPTPTV
                     SETWTDAPAGPVGDERYGGTTTCGAELTRELRGIVHPVLPCGARIVVAFGGREAETEV
                     IDQGPVGAGEEFSLTAPLAAELGVDGLQTVRWRFSTGTG"
     gene            complement(3772..4710)
                     /locus_tag="VFO88_03895"
     CDS             complement(3772..4710)
                     /locus_tag="VFO88_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544696.1"
                     /translation="MSSNEPSRASAAPALTAMPQVQDLPVAEHGYDRAKVEESFEAFR
                     RHVTSLQAQLRVLQAAPRGSGSEPTGQAVRMDALHLVRAAAEFADTIERDAQEAAAKQ
                     IARAEKEIRERHMSVQQDEVEVQRIRQETERQRVEIVNAARAEAREILAKANRDSTQE
                     LREAESSGARLLEQARHQATELTNAARAEVEQTLEWARAQGEVIIQRARLGAEQLLGA
                     AGHGDPAIAEAVEAIVRSAQGAGGPSPGESPGPASAGSGAQERWTPSAAPQQEEPAAE
                     PEPEPEPAAEEPQQPEAEESTEEPAAGQGDESPTSS"
     gene            complement(4782..6524)
                     /gene="aspS"
                     /locus_tag="VFO88_03900"
     CDS             complement(4782..6524)
                     /gene="aspS"
                     /locus_tag="VFO88_03900"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET9544697.1"
                     /translation="MTTHWRDLMCGDVRPEHVGRRLTLAGWADGRRDHGGLVFVDLRD
                     HTGICQLVLNPETDPKAAETGHQIRNEFVLRAEGEVVRRAPENVNPNIPTGEVELQVD
                     RLEIVSRSTPLPFQLDEEGVDETLRLRYRWLDLRRERMQRNIAKRAQMVGIIRRTMEE
                     AGFVDIQTPILFKPTPEGARDFVVPSRLQKGRFFALPQSPQILKQLLVVAGFDRYYQI
                     AICFRDEDLRADRVQEITQLDVEHAFPDQEFLFGLMETMLARIWSDCIGVELETPFPR
                     MTHDDAIARFGSDKPDLRFGLELEDATGVTRGSGFGVFGNAPCVRFLRVPKAFSRSEL
                     AALEEVAKEWGAKGLAYLVTDEAGEVRSPIAKFLSERELEALRSDPGTTLLFGADEPA
                     AVARALGALRLHLGRELGLVDEDAWRPLWVTDFPMFEWDADEERWSAVHHPFTRPAPG
                     SEELVRTDPGAALAHAYDCVINGIEIGGGSFRIHEPELQAAVFDTLALSPEEQRTKFG
                     FLLDALAMGAPPHGGIAFGIERMLMGLLKEPNLRDVIAFPKNQAGVDPMSGAPSEVTG
                     EQLRELGIRLVEKP"
     gene            complement(6521..>7000)
                     /locus_tag="VFO88_03905"
     CDS             complement(6521..>7000)
                     /locus_tag="VFO88_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9544698.1"
                     /translation="LDYYTRTVWEFVNEGLGAAQATICAGGRYDYLVEEIGGPPTPGV
                     GWAAGVERVGMSAGIEPEPPALDVFVVCDAEADRPRVLAEVAKLRAAGVRADSDHAGR
                     SLKGQMTQAGRTRARTIVRVEGETATVRKSGADVPEPIEIRQIAATLAAEAGREAEV"
BASE COUNT          880 a         2582 c         2574 g          964 t
ORIGIN      
        1 acggcgccgt ccttgaagta gtcgccgatc gagaagttcc cgagcatctc gaagaaggtc
       61 aggtggcgcg cggtgaggcc gacctcgtcg atgtccgtcg tgcggaagca cttctggacg
      121 gtcgtgagga gcggggccgg cggctgctcg aggccgagga agtacggctt gagcggctgc
      181 atgccggccg tcgtgaggag gacggacggg tcgtacgtcg cgggcacgag cgaccccgag
      241 gggcggcgca ggtgtccctt gccctcgaag aacgagagga agccctcgcg aagctcgtcg
      301 gaggtgtgca ccgtgcgatc gtaccccgcg gtcggccggg ggccggacgc gcctcggacg
      361 tgcacggatc gtcacgcttt cgcgcgcatt ccggcacgga cgcgtgcggg gtttggcgat
      421 agcgttcgtg ctgggacgct cgctcccccg atggggggcg ctggtccggg cggcgcgccg
      481 gccccgaagg cgcgccggaa cgatcaggcg ttcgggtgga ggtcgctcgc gtcgaccgcg
      541 atctcctcgc ggatcttctc gagcgagcgg cggatgaggc gcgacacgtg catctgcgag
      601 atccccacct gctgggcgat ctgggactgg gtcaggccct cgaagaagcg cagctcgagg
      661 atgcggcgct cgcggtcgtc gagcaccttc cagcccgggg cgagcagggc gcggtcctcg
      721 ctcacctcgt actccttctc gatctcgccg agcgactcga gcgggtcgag gtcgtccccg
      781 tcgtcaccgc cgccggaggg cgcggagagc gagagcgtcg cgtacgcctg cccggtctcg
      841 agggcctcga gcacctcctc ctcctcgacg tcggcggcct tcgcgagctc cgcgatcgtc
      901 ggcgagcgct cgtactggac ggtcagctcc tccatgaggc gcgagagccg cacgttcagc
      961 tcctggagcc ctcgcggcac gcgcaccgcc cagcccttgt cgcggaagtg ccgcttgatc
     1021 tcgccgatga tgttcggcgt cgcgtacgtc gtcagctcga cgccgcggtc gacgtcgaag
     1081 cggtcgatcg ccttgatgag gccgatgcag cccacctgca cgaggtcctc gagctgctcc
     1141 ccgcggtacg agtagcggcg ggcgagcgag cgcacgagcg gcaggtactg ctcgatcagc
     1201 cgctggcgcg cttccacgtc gccctggtgg tggtagcggc ggaggagctc gcggtcgttc
     1261 ttctcgggcg gagcgctcac gccgtgggct ctcccgcggg gatcggcttg cgcatccgga
     1321 gccagccctg gccgtcgcgc gcctcgagct cgtgctcgcc catcgtcgcc gcgagcagcc
     1381 gtccgagcgc gacgccctgc acgtcgtcgg atgcctggag atcgtgctcg agcgcgccgg
     1441 gctcgagcgg cccgaccgcg acggcgaggc tcgagccgtc gagctgcacc tcgacgctca
     1501 cctcgtcccc cacggcgtac gcgtcgttcc cgaggatcgt ctcgacggcg agctggacgt
     1561 cctcgagcgt ctcgtacggg aggtcgagcc gcgcggcgag cccaccgagc acgagccgga
     1621 cgaccccctg gtacgggcgc tcgaacggga cggtgagggt gatcgcttcg cgcatctgga
     1681 cgcctccttg ggtcgctccc gccctccgaa gcagatggac agggccacgg ggagccgtgg
     1741 tcagtgtaga cacggcgccg ggtctacgac gccgacgcga tgagccccgc gccgaccacc
     1801 gcatcggcct cgtagagcac cgccgcttgg cccggcgcga cgccgtacgc gggactgtcg
     1861 aggcgcaggc gaaagccccg cgcgcgcggc tcgacgcgcg ccggcgcgcc cggagagcgg
     1921 tagcggagct tcacctcggc gcgggcgacc gggacgaaga ggcgcccgtt ccggacgccg
     1981 atcgcctgcc gcccgagccg ctcgcgcggg ccgacgacga ccgtgttcgt ccgcgggtcg
     2041 gcccggagcg cgtagagcgg ccggccgccc gctacgccga ggcccttccg ctggccgggc
     2101 gtgaagcgcc agtagccggc gtggcggccg agctcccggc ccgactcgtc ggtgacggga
     2161 ccgtcggcgg ttgcgagacc gtggcgctcg aggaagtcgc ggtagtcgcc gcccgcgagg
     2221 aagcacgcct cctggctctc ggcacgaccc gcggcgggga gcccgtgcgc ggcggcgtac
     2281 gcgcgcgtct cgcccttcgt ccagtctccg agcgggaacg agacccggcc gaggtggcgc
     2341 aggtcgaggc gtgcgagcat gtacgactgg tccttctccg cgtccgcgcc gcgcgcgagg
     2401 agaagacgtc cgcggtgctc gcggaggcgg gcgtagtggc cggtcgcgag ccgctcggcg
     2461 ccgatccggc gggcgaacgc gagcagctcg gcgaagcgga agctcccgtt gcagcggatg
     2521 cacgggttcg gcgtctcgcc gcgcgcgtag ccggcgacga acgggccgac gacggcgcgc
     2581 cggaactcct cccggaggtc gagcgtgaag tgcgggatcc cgagcccctg gcacgtctcc
     2641 cgcgcggcga cgaccgccga cggggagcag cacacacgct cgccgtccgg gcccgcgggg
     2701 tcggtccaga gccggagcgt cacgccgacc ggctcggcgc ccgcctcgcg gacgcgaaga
     2761 agcgcgaccg cgctgtcgac gcccccgctc atggcaacgg cgacccgagc gcggtcacgc
     2821 ggcggacggg cggcggcgac cggcgcgagc gcctcggcca gcgcgtctgc ggcgagcggc
     2881 gccgccggga ccgcggcggc ctcgagcgcg tcgcgcccgc ggatctcccg tgagagcgcg
     2941 gccacgccgg gcccatcccc ctgcgcgtcg acgatgcggc cgcggtcgac cgtcaggcga
     3001 atccatgcga gatcgtctcc gcggacggcg tttccgactg cctcgaccat gcgtccacgg
     3061 tagcccacgc gtgcgcgggc gccgcgcgaa aaaggagatg cgtgcaccac cccgccgatg
     3121 aagccccgcg cgtgaggaac ctggcaagcc aggtgggtgc cctgccggcc ggacgtcatg
     3181 ccgagccgaa cgcgggctcc ctctcagtag ctgttggttc tacattgcgc gcgaggccac
     3241 gcacgcggtc gagcacgcac ccgcactcta gcgcctctgc gcgcggccgc taccccgttc
     3301 cggtggagaa gcgccagcgc accgtctgga ggccgtcgac gccgagctcg gccgcgagcg
     3361 gcgcggtgag cgagaactcc tcccccgccc cgaccgggcc ctggtcgatc acctccgtct
     3421 ccgcctcgcg ccctccgaac gccacgacga tccgcgcccc gcacggcagc accgggtgga
     3481 cgattccgcg cagctcccgc gtcagctcgg ccccgcacgt cgtcgtgccg ccgtagcgct
     3541 cgtccccgac ggggccggcc ggcgcgtccg tccaggtctc ggacaccgtc ggggtcggcg
     3601 acgccgaccc gccgtcctcg ccgtcaccgt cgcggacgag cgccaggctc acgagcgtcg
     3661 cgagcagcgc gaccgccgcg agcgcgagca ggcgttcgag gagctgcccc ctcacgaccg
     3721 gtggtgtccg tcacccgggg cgcccggcat gctgcggcgc tccgcctcgc gctacgaaga
     3781 cgtcgggctc tcgtccccct gcccggcggc cggctcctcg gtgctctcct cggcctcggg
     3841 ctgctgcggc tcctccgccg caggctcggg ctccggctcc ggctcggcgg cgggctcctc
     3901 ctgctgcggg gcggcgctcg gcgtccagcg ctcctgcgcg cccgacccgg cgctcgcggg
     3961 accgggcgac tcgccgggcg acgggccgcc cgcgccctgc gcggagcgga cgatcgcctc
     4021 gaccgcctct gcgatcgcgg ggtcgccgtg gccggccgcc ccgagaagct gctcggcgcc
     4081 caggcgggcg cgctggatga tgacctcgcc ctgcgcccgc gcccactcga gcgtctgctc
     4141 gacctcggcg cgtgccgcgt tcgtgagctc ggtcgcctgg tggcgcgcct gctcgagcag
     4201 ccgggccccg gaggactcgg cctcgcggag ctcctgcgtc gagtcccggt tcgccttggc
     4261 gaggatctcg cgcgcctcgg cgcgcgcggc gttcacgatc tcgacccgct gacgctccgt
     4321 ctcctggcgg atccgctgca cctcgacctc gtcctgctgg acggacatgt gccgctcgcg
     4381 gatctccttc tcggcgcgcg cgatctgctt cgcggccgcc tcctgggcgt cgcgctcgat
     4441 cgtgtcggcg aactcggccg cggcgcgcac gagatggagc gcgtccatcc gcacggcctg
     4501 gccggtcggc tccgatcccg agccgcgcgg cgccgcctgc aggacgcgga gctgcgcctg
     4561 aagcgacgtc acgtggcggc gaaacgcctc gaacgactcc tcgaccttcg cgcggtcgta
     4621 cccgtgctcc gcgaccggca ggtcctgcac ctgcggcatc gcggtcagcg ccggcgcggc
     4681 ggaagcccgg ctcggttcgt tcgaggacat cacgggtcga cctcctaggt cgtgagggtg
     4741 cgatcagtat tgctactcgt cggcgcggac ggcaaccgag tttagggctt ctccacgagc
     4801 cggatcccga gctcgcgaag ctgctcgccc gtcacctcgc tcggcgcgcc gctcatcggg
     4861 tcgacgccgg cctggttctt cgggaaggcg atgacgtcgc ggaggttcgg ctccttgagg
     4921 agccccatga gcatccgctc gatcccgaac gcgatcccgc cgtgcggcgg ggcgcccatg
     4981 gcgagggcgt cgaggaggaa cccgaacttc gtccgctgct cctccggcga gagcgcgagc
     5041 gtgtcgaaga cggccgcctg gagctcgggc tcgtggatcc ggaaggagcc gccgccgatc
     5101 tcgatcccgt tgatcacgca gtcgtacgcg tgggcgagcg ccgcaccggg atcggtgcgc
     5161 acgagctcct cggagccggg cgcggggcgg gtgaacggat ggtggacggc gctccagcgc
     5221 tcctcgtccg cgtcccactc gaacatcggg aagtcggtca cccagagcgg gcgccacgcg
     5281 tcctcgtcca cgaggccgag ctcgcgcccg aggtggagcc ggagcgcgcc gagcgcgcgc
     5341 gcgaccgcgg cgggctcgtc cgcgccgaag aggagggtcg tgcccgggtc ggaccgcaac
     5401 gcctcgagct cgcgctcgga gaggaacttc gcgatcggcg agcgcacctc gcccgcctcg
     5461 tcggtcacga ggtacgcgag ccccttcgca ccccactcct tcgccacctc ctcgagcgcg
     5521 gcgagctccg agcgcgagaa cgccttcggc acgcggagga accgcacgca cggcgcgttc
     5581 ccgaacacgc cgaacccgga gccgcgcgtc accccggtcg cgtcctcgag ctcgagcccg
     5641 aagcgcaggt cgggcttgtc gctgccgaag cgcgcgatcg cgtcgtcgtg cgtcatgcgc
     5701 gggaacggcg tctcgagctc gacgccgatg cagtcgctcc agatccgcgc gagcatcgtc
     5761 tccatgagcc cgaagaggaa ctcctggtcg gggaacgcgt gctcgacgtc gagctgcgtg
     5821 atctcctgca cccggtcggc gcggaggtcc tcgtcccgga agcagatcgc gatctggtag
     5881 taccggtcga acccggcgac gacgaggagc tgcttcagga tctgcggcga ctgcgggagc
     5941 gcgaagaagc ggcccttctg caggcggctc gggacgacga agtcgcgcgc gccctccggg
     6001 gtcggcttga agaggatcgg cgtctggatg tcgacgaagc ccgcctcctc catcgtgcgg
     6061 cggatgatcc cgaccatctg ggcgcgcttc gcgatgttgc gctgcatccg ttcgcggcgc
     6121 aggtcgagcc agcggtagcg gaggcggagc gtctcgtcga cgccctcctc gtcgagctgg
     6181 aacggcagcg gcgtcgagcg gctcacgatc tcgagccggt cgacctggag ctcgacctcg
     6241 ccggtcggga tgttcgggtt cacgttctcg ggcgcgcggc ggacgacctc gccctcggcg
     6301 cgaaggacga actcgttgcg gatctgatgg ccggtctcgg ccgccttcgg gtcggtctcc
     6361 gggttgagga cgagctggca gatcccggtg tgatcgcgca ggtcgacgaa cacgagcccg
     6421 ccgtggtcgc ggcggccgtc cgcccagcct gcgagcgtca gccggcgacc gacgtgctcg
     6481 gggcggacgt cgccgcacat gaggtcgcgc cagtgcgtgg tcaaacctcg gcctcgcgtc
     6541 ccgcctccgc cgcgagggtg gcggcgatct gccggatctc gatcggctcg gggacgtcgg
     6601 cgccgctctt gcgcacggtc gcggtctccc cttccacgcg gacgatcgtg cgcgcccgtg
     6661 tccggccggc ctgggtcatc tggcccttga gcgagcggcc ggcgtggtcg gagtcggcgc
     6721 ggacgccggc ggcgcggagc ttggcgacct ccgcgaggac gcgcggccgg tcggcctctg
     6781 cgtcgcagac gacgaagacg tcgagcgcgg gcggctcggg ctcgatcccg gccgacatgc
     6841 cgacgcgctc gacccccgca gcccagccga cgccgggggt cggcgggccg ccgatctcct
     6901 cgacgaggta gtcgtagcgc ccgcccgcgc agatcgtcgc ctgcgcggcg ccgagcccct
     6961 cgttcacgaa ctcccagacc gtgcgcgtgt agtagtcgag
//
LOCUS       DASTFC010000131         5133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_892357,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000131 DASTFC010000000
VERSION     DASTFC010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892357"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFO88_03910"
     CDS             <1..949
                     /locus_tag="VFO88_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9544699.1"
                     /translation="VTLFHRGRHGADLFPDVERLAGDRDGDLSALAGREWDAVVDTSG
                     YFPRQVRASAEALAGRVGHYAFVSSASVYADHSRVGTREEAPVNEVPEGAPEELSTPE
                     LYGGFKALSERAAEDVLSGRVLVVRAGLVVGPYDPTNRFTYWVTRLARGGETLAPEPR
                     TQPVQLVDARDLAAWILRCAETGTTGTFNATGPTSPLTLGGLLERVRDAVGGRADLVW
                     ADERFLVDAGVEPFTDLPFWLAPTLDPEWAGFLGLDVSRALAAGLTLRPLEETARDTL
                     AWAGREAAPAKDVGVSTAPAGLAPERERELLAAWRKEAA"
     gene            946..2109
                     /locus_tag="VFO88_03915"
     CDS             946..2109
                     /locus_tag="VFO88_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET9544700.1"
                     /translation="MSVAALDVAAVRARFSALDRPTAFLDGPGGTQTPDSVIEAIADY
                     LRRDNANLGGPFATSRASDELVVRAHEVAARFLGGRAEEVGFGQNMTTLNFALTRTLG
                     RELAAGDEIVVTRIDHDANVAPWLHLAQDLDLVVRFCDVHEDTTLDLADLERQLGRRT
                     RVVAFPWASNGVGTITDVRRIADLAHDAGALAWVDAVHYAPHGPIDVREAGADLLLCS
                     PYKFYGPHLGLFWGSEELLDRLRPYKVRPAPDHPAGARFETGTLPHEALAGFVAAVEY
                     VEALGWPAILAHEQVLAERFLAGLPDGLVLHGLPTAEGRTPTFALSVPGHTPHEAAAR
                     LAERDIAVWSGDYYAFEIMNRLGLPDGAVRVGFVHYNTEDEVDRALAALAELL"
     gene            complement(2294..2695)
                     /locus_tag="VFO88_03920"
     CDS             complement(2294..2695)
                     /locus_tag="VFO88_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9544701.1"
                     /translation="MGECRLVRGRGAYRGKQGLEYFEGVSAESAGATGLCLHRLVLPP
                     GARAEPHLHEEHESAVYVLSGEAVMWHGERLEQRMDIRAGDFVYIPAGVPHLPANPSE
                     SEPVVAVIARTDPSEQESVLLRPDLLDVPLR"
     gene            complement(2698..3570)
                     /locus_tag="VFO88_03925"
     CDS             complement(2698..3570)
                     /locus_tag="VFO88_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9544702.1"
                     /translation="MAAGEHMRRAIAAQPEWLRAVPERVGSLRLPAGRVVFVGCGTSF
                     HAAQTGGWAVQALEAVLSPLEADLMVCISHEGETELTLEAARAFGGPSWLVTGKPESP
                     HAELADEVLVVTPEVEKSWCHTASYTCAVAALAVLRGEDVEGVPDAVAAAIADPFPLS
                     GHERWLVAGAGRDWPTAQEAVLKLREGSFVAAEAHQTEQLLHGHLAAVDETVRCFVLE
                     GSGRAADRARQAAAALEELGCDVTLVPTVHPVADVVRFQTLTLALAEAKGIDPDWIHR
                     DDERWKRAAARHKG"
     gene            3597..4016
                     /locus_tag="VFO88_03930"
     CDS             3597..4016
                     /locus_tag="VFO88_03930"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9544703.1"
                     /translation="MDGFRIVHAQPVEFRDLDGLGHVNNAVYLNYLENGKIAYFRDVV
                     GAADLQHLGIVADVKIAYRSPAFLGEELAVGVRVGRLGTKSMEFEFEVRGRDGRLVAE
                     GTSVHVAFDYDRLEPIPVPDEWRRRIESHESESLAAT"
     gene            4013..>5133
                     /locus_tag="VFO88_03935"
     CDS             4013..>5133
                     /locus_tag="VFO88_03935"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9544704.1"
                     /translation="MSQPVFEKNVLSNGLRVVTADMPQAKSVACFVMLAAGSRYETKD
                     TNGIAHFAEHMFFKGTERRPNARDIAGEIDSIGGEFNAFTGKEYTGYYVKCAAEHRDV
                     ALDVLVDMLRNSKFDPEEIEREKGVIVEEMNMYYDTPRDYLEGVYDELLYGDQPLGWD
                     IIGTKETVRAATRETFLGYLDRWYRSSRMVAGVAGALGGDVLERVESLLGDVPDGETG
                     SPEPASPLLNGRRVKIHHKPSDQAHIRVGVPSYPIDHPDRYALNVLATVLGGGMSSRL
                     FSEVRERRGLAYYIYGYNQGYTDTGTLFAQGGVDIARIDEAISTVVTEFGRIAAEPVS
                     DDELQKARNFAKGRLVLSLEDPRGTILFGLRSEVLEGKIR"
BASE COUNT          703 a         1931 c         1827 g          672 t
ORIGIN      
        1 ggtgacgctc ttccaccgcg gccgccacgg cgccgatctc ttcccggacg tcgagcggct
       61 ggccggcgac cgggacggcg acctctccgc gctcgcgggc cgcgagtggg acgcggtcgt
      121 ggacacgtcg ggctacttcc cgcgccaggt gcgcgcgtcc gcggaggcgc tcgcggggcg
      181 cgtcggccac tacgcgttcg tctcgagcgc ctcggtctac gccgaccact cgcgcgtcgg
      241 gacgcgcgag gaggcaccgg tgaacgaggt gccggagggc gcgccggagg agctctcgac
      301 gcccgagctc tacgggggct tcaaggcgct ctcggagcgc gccgccgagg atgtgctctc
      361 cggccgcgtg ctcgtggttc gcgcagggct cgtcgtaggc ccgtacgacc cgacgaaccg
      421 atttacgtac tgggtcacgc ggctcgcgcg cggcggcgag acgctcgcgc cggagccgcg
      481 cacgcagccg gtgcagctcg tggacgcgcg cgacctcgcc gcctggatcc tccgctgcgc
      541 cgaaaccggg acgaccggga cgttcaacgc gaccgggccc acgtcgccgc tcacgctcgg
      601 cgggctcctc gagcgcgtcc gggacgcggt cggcggccgc gccgacctcg tctgggcgga
      661 cgagcgcttc ctcgtcgacg cgggcgtcga gccgttcacc gacctgcctt tctggctcgc
      721 gccgacgctc gaccccgagt gggcggggtt cctcggcctc gacgtctcgc gcgcgctcgc
      781 cgcgggcctg accctccgac cgctcgagga gactgcccgc gacacgctcg cctgggccgg
      841 gcgcgaggcg gcgcccgcga aggacgtcgg cgtctcgacg gcgcccgccg gcctcgcgcc
      901 ggagcgcgag cgcgagctgc tcgccgcctg gcggaaggag gccgcgtgag cgtcgccgcg
      961 ctcgacgtcg ccgcggtgcg cgcgcgcttc tcggcgctcg accggcccac cgcgttcctc
     1021 gacgggcccg ggggcaccca gacgcccgac tccgtgatcg aggcgatcgc cgactacctc
     1081 cggcgcgaca acgcgaacct cggcgggccg ttcgcgacca gccgggcgag cgacgagctc
     1141 gtcgtccgcg cgcacgaggt cgcagcgcgc ttcctcggcg gccgcgcgga ggaggtcggg
     1201 ttcgggcaga acatgacgac gctcaacttc gcgctgacgc ggacgctcgg gcgggagctc
     1261 gccgcgggcg acgagatcgt cgtcacgcgg atcgaccacg acgcgaacgt cgcgccctgg
     1321 ctccacctgg cgcaggacct cgatctcgtc gtccgcttct gcgacgtgca cgaggacacg
     1381 acgctcgacc tcgccgacct cgagcggcag ctcggccggc gcacgcgcgt cgtcgccttc
     1441 ccgtgggcgt cgaacggcgt cgggacgatc accgacgtcc gccggatcgc cgacctcgcg
     1501 cacgacgcgg gcgcgctcgc ctgggtggac gccgtccact acgcgccgca cgggccgatc
     1561 gacgtgcgtg aggccggcgc cgatctgctc ctctgctcgc cgtacaagtt ctacgggccg
     1621 cacctcgggc tcttctgggg cagcgaggag ctgctcgacc ggctccggcc gtacaaggtc
     1681 cgccccgcgc ccgaccaccc ggccggcgcg cgcttcgaga ccgggacgct cccgcacgag
     1741 gcgctcgccg gcttcgtcgc cgcggtcgag tacgtggagg cgctcggctg gccggcgatc
     1801 ctcgcgcacg agcaggtgct cgcggagcgg ttcctcgccg gccttccgga cgggctcgtc
     1861 ctgcacgggc tcccgaccgc ggagggccga acgccgacgt tcgcgctctc cgtcccgggc
     1921 cacacgcccc acgaggcggc cgcccggctc gcggagcgcg acatcgcggt ctggtcgggc
     1981 gactactacg ccttcgagat catgaaccgg ctcgggctcc ccgacggcgc ggtccgcgtg
     2041 ggcttcgtcc actacaacac cgaggacgag gtcgaccgcg cgctcgcggc gctcgccgag
     2101 ctcctctagg cggtgcgggg cacgagccgc ccgcccgcgt ggaggacgcg gccggtggcg
     2161 agaacgatca tgtagacgag gatccgcatg tgaagctcct tctcgggtga cctccgtagc
     2221 ttcgcccgcc gcgaacgccg gcgccctcgt gccggcgcga gacctgcggt acggctgcct
     2281 ccccgatcgc cggtcagcgc agcggcacgt cgagcaggtc gggccgaagg aggacgctct
     2341 cctgctcgct cgggtcggtc ctcgcgatca ccgcgacgac cggctcggat tccgacgggt
     2401 tcgccggcag gtgcggcacg ccggccggga tgtagacgaa gtcgccggcc cggatgtcca
     2461 tccgctgctc gaggcgctcc ccgtgccaca tgaccgcctc gccgctcagg acgtacacgg
     2521 ccgactcgtg ctcctcgtgc aggtgcggct ccgcgcgggc gcccggcggg agcacgagcc
     2581 ggtgcaggca gagccccgtc gcgccggcgc tctccgcgga cacgccctcg aagtactcga
     2641 gcccctgctt gccgcggtac gcgcctcgtc cgcgcacgag ccggcactcg cccacggtca
     2701 ccccttgtgc cgggcggcgg cgcgcttcca gcgctcgtcg tcgcggtgga tccagtcggg
     2761 atcgatcccc ttcgcctccg cgagggcgag cgtgagcgtc tggaagcgga cgacgtccgc
     2821 gacggggtgc acggtcggaa cgagggtgac gtcgcagccg agctcctcga gcgccgcggc
     2881 cgcctgccgc gcccggtcgg cggcgcgccc ggagccctcg aggacgaagc agcgcaccgt
     2941 ctcgtcgacc gcggcgagat gcccgtggag gagctgctcg gtctggtgcg cctccgccgc
     3001 gacgaacgag ccctcgcgga gcttcagcac cgcctcctgc gcggtcggcc agtcgcgccc
     3061 cgcccccgcg acgagccagc gctcgtgccc ggagagcggg aacggatcgg cgatcgccgc
     3121 cgcgaccgcg tccggcacgc cctcgacgtc ctcgccgcgc agcacggcga gcgccgcgac
     3181 cgcgcaggtg tacgacgcgg tgtggcacca ggacttctcg acctcgggcg tgacgacgag
     3241 cacctcgtcc gcgagctccg cgtgcggcga ctcgggcttg ccggtcacga gccacgaggg
     3301 gccgccgaac gcgcgcgccg cctcgagcgt gagctccgtc tcgccctcgt gcgagatgca
     3361 gaccatgagg tcggcctcga gcggcgagag caccgcctcg agcgcctgca cggcccaccc
     3421 gcccgtctgc gccgcgtgga acgacgtccc gcagccgacg aagacgaccc gacccgcggg
     3481 aagccgcagc gaccccaccc gctcgggcac cgcgcgcagc cactccggct gcgccgcgat
     3541 cgcccgccgc atgtgctccc cggcagccat ccggcgcgaa gctaccatcc agcgcgatgg
     3601 acgggttccg catcgtgcac gcccagccgg tcgagtttcg cgacctcgac gggctcggcc
     3661 acgtgaacaa cgccgtctac ctgaactacc tcgagaacgg gaagatcgca tacttccgcg
     3721 acgtcgtcgg cgcagccgac cttcagcacc tcgggatcgt cgccgacgtg aagatcgcct
     3781 accgctcgcc ggcgttcctc ggggaggagc tcgcggtcgg cgtgcgcgtc ggccggctgg
     3841 gcacgaagag catggagttc gagttcgagg ttcgaggccg cgacgggcgg ctcgtcgcgg
     3901 aggggacgag cgtgcacgtc gccttcgact acgatcggtt ggagccgatc cccgttccgg
     3961 acgagtggcg ccgcaggatc gagagccacg agtccgaatc actcgccgcg acatgagcca
     4021 gcccgtcttc gagaagaacg tcctgtccaa cggcctgcgc gtcgtcaccg ccgacatgcc
     4081 gcaggccaag tccgtcgcgt gcttcgtcat gctcgcggcc ggctcccgct acgagacgaa
     4141 ggacacgaac ggcatcgcgc acttcgccga gcacatgttc ttcaagggga ccgagcggcg
     4201 cccgaacgcg cgcgacatcg cgggcgagat cgactcgatc ggcggcgagt tcaacgcgtt
     4261 cacgggcaag gagtacacgg gctactacgt gaagtgcgcg gccgagcacc gcgacgtcgc
     4321 gctcgacgtc ctcgtcgaca tgctgcgcaa ctcgaagttc gaccccgagg agatcgagcg
     4381 cgagaagggc gtgatcgtcg aggagatgaa catgtactac gacacgcccc gcgactacct
     4441 cgagggcgtg tacgacgagc tcctctacgg cgaccagccg ctcggctggg acatcatcgg
     4501 cacgaaggag acggtgcgcg ccgcgacgcg cgagacgttc ctcggatacc tcgaccgctg
     4561 gtaccgctcg agccgcatgg tcgcgggcgt cgcgggcgcg ctcggcggcg acgtcctcga
     4621 gcgggtcgag agcctgctcg gcgacgtccc ggacggggag accggctcgc ccgagcccgc
     4681 ctccccgctc ctgaacggcc gccgggtgaa gatccaccac aagccttccg accaggcgca
     4741 catccgcgtc ggcgtgccga gctacccgat cgaccatccc gaccgatacg cgctgaacgt
     4801 cctcgcgacc gtgctcgggg gcgggatgtc ctcgcgtctc ttcagcgagg tgcgcgagcg
     4861 gcgcgggctc gcgtactaca tctacggcta caaccagggc tacacggaca ccggcacgct
     4921 cttcgcccag ggcggcgtcg acatcgcgcg gatcgacgag gcgatctcga cggtcgtgac
     4981 ggagttcggc cggatcgcgg cggagccggt gtcggacgac gagctccaga aggcacggaa
     5041 cttcgccaag ggccggctcg tgctctcgct cgaggacccg cgcgggacga tcctcttcgg
     5101 cctgcggagc gaggtgctcg aggggaagat ccg
//
LOCUS       DASTFC010000132         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_315506,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000132 DASTFC010000000
VERSION     DASTFC010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315506"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFO88_03940"
     CDS             complement(<1..621)
                     /locus_tag="VFO88_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544705.1"
                     /translation="MTLPLYAGGFLGPFGGAMLIALIPNVAEGLDTSLTLVAAAITTY
                     MVPFAVLQLVSGTVAERLGARRVVRVAYVVFGLAALACALAPEIWTFLGARAVMGAAN
                     AFLSPILLAALSEVVAPEVLGRTVGTFAAWQTAGFTFAPALGGALGEVSWRLAFAAVT
                     VAALALAVPTRTLGSFERQPGEGHASIRGLVSGWFGALAAKALLGYL"
     gene            699..1946
                     /locus_tag="VFO88_03945"
     CDS             699..1946
                     /locus_tag="VFO88_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544706.1"
                     /translation="MDSDLSLQRAVRRNTLLLATSTAVTSATMQLVAALASTTFVIAT
                     GIEGLLGLGPAIFLASSASAALPAGRAMDRFGRKPVIAVGFALGAIGTTVTAFAVGAE
                     LGIGVVLGFALIGIAAGTVLLARAAAGDMYPPERRARGISLVLFGSVFGAILGPAVFG
                     PIFSGRDLTADSLVVPWLAGGGFMVAGLLLILLVRPDPKTIGERLFPAAEGVAPAPPA
                     PLRELVRRPGVPSALLAAFSSFGVMVAVMNLTGYAVVHEHGHSQSSVFPIIGAHVFGM
                     FALVLVIGQIVDRFGGSVVLVAGLALEGVSSLGLAWAEGVWATALLLFGLGLGWNLAF
                     VAATTALVNRTAPAERGKLVGFSDLSSGLVGATLAILGGWILQGWGVGALAAGATVVA
                     FLPALFLARSFLLPPRRPVGEAA"
     gene            1943..>2305
                     /locus_tag="VFO88_03950"
     CDS             1943..>2305
                     /locus_tag="VFO88_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544707.1"
                     /translation="MSLVEELARARIGATFNFYRDGEGAGLRRDRLRAYLEARADAPA
                     LLVAEAPGYRGTRVSGVPLTSERQLTGSGPAEATATIVHRALAETGLSQDVLLLNVVP
                     THPHLPGRQETNRRPTRAE"
BASE COUNT          261 a          884 c          842 g          318 t
ORIGIN      
        1 gaggtagccg agcagcgcct tcgccgcgag cgcgccgaac cagccggaga cgagcccgcg
       61 gatgctcgcg tgcccctcgc cgggctgccg ctcgaaggag ccgagcgtcc gggtcggcac
      121 cgcgagcgcg agcgccgcga cagtcaccgc ggcgaaggca agccgccacg acacctcgcc
      181 gagcgccccg ccgagcgcgg gcgcgaacgt gaacccggcc gtctgccacg ccgcgaacgt
      241 tccgaccgtc cggccgagca cctccggcgc gaccacctcc gagagcgcgg ccagcaggat
      301 cggcgagagg aacgcgttcg ccgcgcccat gaccgcccgg gcgccgagga acgtccagat
      361 ctccggcgcg agcgcacacg cgagcgctgc gaggccgaag acgacgtacg cgacgcggac
      421 gacccggcgc gcgccgagcc gctccgccac tgtcccggac acgagctgga gcaccgcgaa
      481 cgggaccatg tacgtcgtga tcgccgccgc cacgagcgtg agcgacgtgt cgagcccctc
      541 cgcgacgttc gggatgagcg cgatgagcat cgcgcccccg aacggcccga ggaacccgcc
      601 cgcgtagaga ggaagcgtca cgcgcacgac ggcagtgttc cctgcgaggc atgcctcgcg
      661 ttacgccgtc gttacgagcg tttgtgagac tggcgtcgat ggactccgat ctctccctgc
      721 agcgcgccgt ccggcgcaac acgctgctgc tcgcgacgtc caccgccgtg acgtccgcga
      781 cgatgcagct cgtcgccgcg ctcgcctcga cgacgttcgt gatcgcgacc gggatcgagg
      841 gcctgctggg gctcgggccg gcgatcttcc tcgcgagctc cgcgtccgcc gcgctccccg
      901 cggggcgcgc gatggaccgg ttcgggcgca agcccgtcat cgcggtcggg ttcgcgctcg
      961 gggcgatcgg gacgacggtg acggcgttcg ccgtcggtgc ggagctcggg atcggcgtcg
     1021 tcctcggctt cgcgctgatc gggatcgccg ccggcaccgt gctcctcgcc cgcgcggcgg
     1081 ccggcgacat gtacccgccg gagcggcgcg cgcgcgggat ctcgctcgtc ctcttcggct
     1141 ccgtcttcgg cgccatcctc gggcccgccg tgttcgggcc gatcttctcc ggccgcgacc
     1201 tgaccgcgga ctcgctcgtc gtcccgtggc tcgccggggg cggcttcatg gtcgccgggc
     1261 tcctgctcat cctgctcgtg cggccggacc cgaagacgat cggcgagcgg ctgttcccgg
     1321 ccgcggaggg ggtcgcgccg gccccgcccg cgccgcttcg cgagctcgtc cgccgccccg
     1381 gggtgccctc cgcgctcctc gccgcgttct cgagcttcgg cgtcatggtc gccgtcatga
     1441 acctgaccgg ctacgcggtc gtccacgagc acgggcactc gcagagctcg gtgttcccga
     1501 tcatcggcgc gcacgtcttc gggatgttcg cgctcgtgct cgtgatcggg cagatcgtcg
     1561 accgcttcgg cgggagcgtc gtgctcgtcg ccgggctcgc gctcgagggc gtctcgtccc
     1621 tcgggctcgc ctgggcggag ggcgtgtggg cgaccgcgct cctcctcttc gggctcgggc
     1681 tcggctggaa cctcgccttc gtggccgcga cgaccgcgct cgtgaaccgg acggcgccgg
     1741 ccgagcgggg gaagctcgtc ggcttcagcg acctctcgtc cgggctcgtc ggcgcgacgc
     1801 tcgccatcct cggcggctgg atcctgcagg gctggggcgt cggcgcgctc gccgccggcg
     1861 cgaccgtggt cgcgttcctg ccggccctct tcctcgcccg gtcgttcctc ctgccgccgc
     1921 ggaggccggt cggcgaggcg gcgtgagcct cgtcgaggag ctcgcccgcg cgcggatcgg
     1981 cgcgacgttc aacttctacc gggacggcga gggcgccggg ctgcgacgcg atcggctgcg
     2041 cgcgtacctc gaggcgcggg ccgacgcgcc cgcgctgctc gtggccgagg cgcccggcta
     2101 ccgcgggacg cgcgtgagcg gcgtgccgct cacgtccgag cgccagctca cgggctcggg
     2161 gccggcggag gcgaccgcga cgatcgtcca ccgcgcgctc gcggagaccg ggctctcgca
     2221 ggacgtgctc ctcttgaacg tagtgccgac gcatccgcac ctcccgggcc gtcaggagac
     2281 gaaccggcgc ccgacccgcg cggag
//
LOCUS       DASTFC010000133         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1518793,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000133 DASTFC010000000
VERSION     DASTFC010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518793"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFO88_03955"
     CDS             complement(<1..708)
                     /locus_tag="VFO88_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9544708.1"
                     /translation="MPPFSKILVANRGEIAVRVFRTLRELGIASVAVYSEADRSALHA
                     RAADEAYLVGPAAPAESYLNGERIVETARRAGAEAIHPGYGFLAENAGFARLCEEAGI
                     VWIGPPPEAIEAMGSKVEARRLMTKAGVPVIPGTTDPVETADELVRLGDEYGWPVAVK
                     ASAGGGGKGLKVVASAAEAERALASARREGEAYFSDPTVYVEKLILDPRHVEVQVLAD
                     AHGNAVHLGERDCTIQRR"
     gene            809..1348
                     /gene="pgsA"
                     /locus_tag="VFO88_03960"
     CDS             809..1348
                     /gene="pgsA"
                     /locus_tag="VFO88_03960"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9544709.1"
                     /translation="MTFADQLTVARALAVPVVVALFAWDFTGHDYWATAVFAVAMATD
                     WVDGWWARRSGRTSDLGRLLDPVADKLLVMAVLIMLVGPVFPAWMVAAVIAREFLVSG
                     LRLAALERGVVIGARDLGKLKTWSQAIAAGLGGLAAGGAVSTDVAWVALLVAVILTWV
                     SGLDYARVAPTLLRGRQPA"
     gene            1329..2213
                     /locus_tag="VFO88_03965"
     CDS             1329..2213
                     /locus_tag="VFO88_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9544710.1"
                     /translation="MAVSLRSYRPKDAERVVELSRLTLARPEFQVGNPIWTTLEELES
                     ELATWEPPAEETLLVASDGRRIVAFGGVELPAGFPHAELFGPLVAAESRGHRLGTALL
                     EASVERARAGGAQAVLAAVGTRNAAGRILLERHGFRTEGRPQATFRLRPEDHRPLEDP
                     PEGVEIRVAGADDLPAALALYRECFPQGRFPDEVWRENVGTGSVYAAEVDGELVAVLN
                     IDPSDRWIYHVGVTQTERNRGVGSYLLSRSLQDYWQEHPGQPLGLDVDASNVPAIRLY
                     RRQGFAPWLVLQTFELEL"
     gene            complement(2210..2392)
                     /locus_tag="VFO88_03970"
     CDS             complement(2210..2392)
                     /locus_tag="VFO88_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544711.1"
                     /translation="MPGEWSEGELLIAPEPRDGGRAAIRAALRQTAERRRPGERTAWW
                     REGIRANLRPELRPGR"
     gene            2480..3487
                     /locus_tag="VFO88_03975"
     CDS             2480..3487
                     /locus_tag="VFO88_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET9544712.1"
                     /translation="MRRPAAALAAVAAALVLAPAATAASWAQPQIKIAVKSGLMGPSV
                     DRFRPAATLTRGELAQIVGGLSGTAKTPADPTRPVTMTQLNRAVVRALGLGPAAAKIQ
                     AELVRRKLEPPPRAGWETVARLLELRFNHPKERDDRERRPADPATRAEAAYTTAQALG
                     LSSWQRDRARERAASFRLPALTTWQRKVLRRAMRFVGYPYVWGGMSENRQTLFGVTSR
                     GGFDCSGFVWRVYKLEPWPGAPKLNTVIRGRTTYDMSGEVGLSKRYGRAKLRPADVIF
                     FGSNGRRSRPSQVTHAGIAMGGGWFVHSSGNGTTIAPLEGWYVDTFAWARRPLAEAGL
                     A"
     gene            3795..>4531
                     /locus_tag="VFO88_03980"
     CDS             3795..>4531
                     /locus_tag="VFO88_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544713.1"
                     /translation="MTTAGLATLAAALVTQQQAGLGPVEVGGLLLLVGGATLAERFPV
                     PIEIEGMPAGGVSLAAVFIVGAAVLYGWAPAVIVAFLAAAVVHFAQKRPFDRLLYNGA
                     AYALSAGAAGIAAGAHQTRTGTVALVVAVLAASAAFYATNVALIAAIVARMAGERLVG
                     LVARSIRSTVIPFGIMASVSLTLTVLWHHSPLLSAALVGPLVAVALYQRSVHEALAAM
                     RLALTDPLTGLGNHRHFHERLQSDLDRA"
BASE COUNT          535 a         1730 c         1614 g          652 t
ORIGIN      
        1 ccggcgctgg atcgtgcagt cgcgctcgcc gaggtgcacg gcgttcccgt gcgcgtcggc
       61 gaggacctgc acctcgacgt gccgcggatc gaggatgagc ttctcgacgt agacggtcgg
      121 gtcggagaag tacgcctccc cttcgcggcg cgccgatgcg agcgcccgct ccgcctccgc
      181 cgcggaagcg acgaccttga ggcccttccc gccgccgccc gccgacgcct ttaccgcgac
      241 gggccagccg tactcgtcgc caagccggac gagctcgtcc gccgtctcga ccgggtcggt
      301 cgtgccgggg atcacgggca ccccggcctt cgtcatgagc cttcgcgcct cgaccttcga
      361 ccccatcgcc tcgatcgcct ctggcggcgg cccgatccag acgatccccg cctcctcgca
      421 caggcgcgcg aagccggcgt tctcggcgag aaacccgtac ccggggtgaa tcgcctcggc
      481 gccggcgcgc cgggccgtct cgacgatgcg ctcgccgttc aggtagctct cggcgggcgc
      541 cgccgggccg acgaggtacg cctcgtcggc cgcgcgggcg tggagggcgc tccggtcggc
      601 ctcggagtag accgcgacgg aggcgatccc gagctcgcgg agcgtgcgga agacccggac
      661 ggcgatctcg ccccggttcg cgacgaggat cttcgagaac ggcggcacga tgccgacatt
      721 ctggatgctt tcggcccgtc ggcgcgcggc tcgcggccct cgtcgcgccg cccggccggc
      781 ggggcgcggg cggcgtatcc tccacgaagt gaccttcgcc gaccagctca cggtcgcccg
      841 ggcgcttgcg gtccccgtcg tcgtcgccct cttcgcctgg gacttcaccg gccacgacta
      901 ctgggccacg gccgtgttcg cggtcgcgat ggccaccgac tgggtggacg ggtggtgggc
      961 gcgccgctcc gggaggacgt ccgacctcgg gcggctcctc gacccggtcg cggacaagct
     1021 cctcgtgatg gccgtcctga tcatgctggt cgggccggtc ttccccgcct ggatggtcgc
     1081 tgccgtgatc gcgcgcgagt tcctcgtgag cggcctccgc cttgccgcgc tcgagcgggg
     1141 cgtcgtgatc ggcgcgcgcg acctcgggaa gctcaagacg tggtcgcagg cgatcgccgc
     1201 cgggctcggc gggctcgccg cgggcggcgc cgtctcgacg gacgtcgcct gggtcgccct
     1261 gctcgtcgcc gtcatcctca cgtgggtctc cggactcgac tacgcacgcg tcgccccgac
     1321 gctcctccgt ggccgtcagc ctgcgtagct accggccgaa ggacgccgag cgggtcgtcg
     1381 agctcagccg cctgaccctc gcccggcccg agttccaggt cgggaacccg atctggacga
     1441 ccctcgagga gctagagagc gagctcgcca cctgggagcc cccggcggag gagacgctcc
     1501 tcgtcgcctc ggacggccgt cgcatcgtcg cgttcggcgg agtcgagctt ccggcgggat
     1561 ttccgcacgc ggagctcttc ggcccgctcg tcgcggcgga gtcgcgcggg caccggctgg
     1621 gcaccgcgct cctcgaggcc tccgtcgagc gggcacgcgc cggcggcgcc caggccgtcc
     1681 tcgccgccgt cggcacgcga aacgcggccg gacgcatcct gctcgagcgc cacggcttcc
     1741 ggacggaggg ccggccgcag gcgaccttcc gcctccgccc ggaagaccac cgcccgctcg
     1801 aggacccgcc ggagggcgtc gagatccggg tcgcgggcgc cgacgacctc cccgccgccc
     1861 tcgcgctcta ccgcgagtgc ttcccgcagg gccggttccc ggacgaggtc tggcgcgaga
     1921 acgtcggaac cggttccgtg tacgcggccg aggtggacgg cgagctcgtc gccgtcctga
     1981 acatcgaccc gagcgaccgc tggatctacc acgtcggcgt cacgcagacg gagcgcaacc
     2041 gcggcgtcgg gtcgtacctc ctgagccgat cgcttcagga ctactggcag gagcacccgg
     2101 gccagccgct cgggctcgac gtcgacgcga gcaacgtgcc cgcgatccgg ctctaccggc
     2161 gccagggctt cgcgccctgg ctcgtcctcc agaccttcga gctcgagctc tagcgtccgg
     2221 gcctgagctc cgggcggagg ttcgcgcgga tcccctcgcg ccaccaggcc gtccgctcgc
     2281 ccggccgccg gcgctccgcg gtctgccgga gcgcggcccg gatcgccgcg cgcccaccgt
     2341 cccggggctc gggcgcgatg agcagctccc cttcgctcca ttcccctggc acggggccga
     2401 ttctcgccga cgcggcgggc ctccgcaaac gagggggatg cgggtaaagg cccgcgaggc
     2461 gcgctccgat aggaaccccg tgcgccgccc tgccgcagcc ctcgccgccg tcgccgccgc
     2521 gctcgtcctc gcgcccgcag cgacggccgc ctcgtgggcc cagccgcaga tcaagatcgc
     2581 ggtgaagagc ggcctcatgg ggccgagcgt cgaccgcttc cggccggccg cgacgctgac
     2641 ccgcggcgag ctcgcgcaga tcgtcggcgg cctctcgggc acggcgaaga cgcccgccga
     2701 cccgacgcgt cccgtcacga tgacgcagct gaaccgggcc gtcgttcgcg cgctcgggct
     2761 ggggccggca gcagccaaga tccaggccga gctcgtgcgg cggaagctcg agccgccgcc
     2821 cagggcgggc tgggaaaccg tggcgcggct cctcgagctc cgcttcaacc atccgaagga
     2881 acgcgacgac cgcgagcgga ggccggccga tccggcgacg cgcgccgagg ccgcgtacac
     2941 gaccgcccag gcgctcgggc tctcttcctg gcagcgcgac cgggcgcggg agcgcgcggc
     3001 gtccttccgg ctccccgcgc tgacgacgtg gcagcggaag gttctccgcc gcgcgatgag
     3061 attcgtcggc tacccgtacg tctggggcgg catgtcggag aaccgccaga cgctcttcgg
     3121 cgtgacgtcc cgcggcgggt tcgactgctc gggcttcgtc tggcgcgtct acaagctcga
     3181 gccgtggccg ggcgccccga agctgaacac cgtcatccgc ggccgcacga cctacgacat
     3241 gtcgggcgag gtcggcctct cgaagcggta cggccgcgcg aagctccgcc cggccgacgt
     3301 catcttcttc ggctcgaacg gccgccggtc gcgaccctcg caggtcacgc acgccgggat
     3361 cgcgatgggc ggaggctggt tcgtccattc gtccggcaac gggacgacga tcgcgcccct
     3421 cgaggggtgg tacgtcgaca cgttcgcgtg ggcccgccgg cccctcgccg aagccggcct
     3481 cgcctagaac gccgccggcg aaagatcccc cgtcggaggg atgatgcgcc cctcccccat
     3541 ctggatactg gccgcactgg cgtaaccgcg tgggaggagg accatgttgc actggaagag
     3601 caggctcacg atcggcctgg ctgcggcgct cgtagtgctc agcgccatcg gcggcttcgg
     3661 ctggagctgg tcgtagacac gtcgtcgacg tggccgacct cgccgcgcac tccctgaggt
     3721 tcgggctccg gagcgtctgg gcgggtacgc gtacgcgccg cccggatgct cggatcctcc
     3781 ccatcctcgt tcccgtgacg acggcgggcc tcgcgacgct cgcggcggcc ctcgtcacgc
     3841 agcagcaggc ggggctcggg cccgtcgagg tcggagggct tcttctcctc gtcggcgggg
     3901 cgacgctcgc ggagcggttc cccgtgccga tcgagatcga ggggatgccc gccggcggcg
     3961 tgtcgctcgc ggccgtgttc atcgtcggcg cggccgtgct ctacggctgg gcgccggcgg
     4021 tgatcgtcgc gttcctcgcg gccgcggtcg tgcacttcgc gcagaagcgg ccgttcgacc
     4081 ggcttctcta caacggcgcc gcgtacgcgc tgagcgcggg cgcggcggga atcgccgccg
     4141 gtgcgcacca gacgcgcacc ggaaccgtgg ccctggtggt cgccgtcctt gcggcgtcgg
     4201 cggccttcta cgccacgaac gtcgcgctca tcgcggcgat cgtcgcccgc atggcgggcg
     4261 agcgtctcgt cggcctcgtc gctcggtcga ttcgctcgac cgtgatcccg ttcgggatca
     4321 tggcgtccgt cagcctcacg ttgaccgtgc tctggcatca ctcgccgctg ctctcggccg
     4381 ccctcgtggg gccgctcgtt gccgtggcgc tgtaccagcg ttccgtgcac gaggcgctcg
     4441 cggcgatgcg tctcgccctc acggacccgc tgaccgggct cggcaaccac cgccacttcc
     4501 acgagcggct gcagagcgac ctcgaccggg c
//
LOCUS       DASTFC010000134         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_266179,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000134 DASTFC010000000
VERSION     DASTFC010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266179"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1350
                     /locus_tag="VFO88_03985"
     CDS             64..1350
                     /locus_tag="VFO88_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9544714.1"
                     /translation="MSTRHPRQPAGLNRREFLQRSAGGAIALSAFAPLLAACGGADDN
                     GGGNGGNGGGGDDGRPELQLARPDNPVEWQLFDDNPPIESDLEPESGTLRIYNWNDYL
                     WPRMLRRFEKEYGVTVELTTFSTADEATAKLTSGAVDFDVYFPTPDRLGRLVLGQILQ
                     PLNLDYIPNLQNVWPSLQNPFYDQGSRYTVPYTIYTTGIGYRADEIAEPPDAYGNPYD
                     VYFDERNAGKMYLLDDSREAIAHMLLRAGVTDVNTEDPEAIEAAKQELISLIDLVNVK
                     LSTNDYTYIPEGRATIHQAWSGSLISAPYYLPQGTGPEVLGFWFPQDGRGVIGNDSMA
                     IPKSAQNPVLAHHFLNFLLDEQNGYDNFVNYVGYQPPFTSIDPDRLVTDEVVPPNLES
                     VVVRESDFDVAGFLLELSPEGANTWQNAWAEFKAGV"
     gene            1343..2260
                     /locus_tag="VFO88_03990"
     CDS             1343..2260
                     /locus_tag="VFO88_03990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9544715.1"
                     /translation="MSEPGRGRRRRGGLLWPILALPGIVWLCLFFLVPFYVILGVAFG
                     TVDPLFLTPEPEWNPARWDPHAFEFVWEGLAAGGGFRTVFLRTFAYVAIAVVISLAIA
                     YPVAYYVARFGGRFKGLLLAGLVAPFFISYLMRMLAWINLLQEDGWVNSVLVWAHVLD
                     APRTWLDGRASSVILGLVYGYVPFMILPLYAFLDRVDRSLLEAARDLGASRFHAFRLV
                     TLPLSWPAVLAGCVIIALPMFGDYYTPDLLSGSPNTSLLGNQIDLYIRGGQQVPVGAA
                     LVVCLMLLLAVFMAYYLWATARAERRLGR"
     gene            2257..3120
                     /locus_tag="VFO88_03995"
     CDS             2257..3120
                     /locus_tag="VFO88_03995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9544716.1"
                     /translation="MRRWLRNAWGKPRFLVLFTLAYFAWSIVPILVAVRFSFNEGRSR
                     STAQGWSTRWYWDDPDLSVRADPDLFGALTQSLTLAAIAVVVTVPLGVALAVGLTRWR
                     GRLATASRFGSMVPLVTPEIVMGVALLLVFVHVFEFVPLGTEAQAIGHVTFSLAFVLI
                     IVRGRLLSIGPEYEEAARDLGASPLQSLRLALFPLLVPAIVASAIIVFAISMDDFVVS
                     NYLSAGAATDTVPVKIYSSGRTAPTPALNALASLTLGVTIAIALVLFVVWRLVRRRRG
                     AEEPTAEAFAA"
     gene            3201..3566
                     /locus_tag="VFO88_04000"
     CDS             3201..3566
                     /locus_tag="VFO88_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544717.1"
                     /translation="MRRRFVTALAVLASGSVLVAGCGASDDQADADRSTASVATSSIA
                     RAPERAATGKPSKWVSIEDCEAVEEILATVEAGRLPDIREQLVSLRLERSSKIRLTED
                     GLLAWLGGSGIGNGTCTFD"
     gene            complement(3610..>3905)
                     /locus_tag="VFO88_04005"
     CDS             complement(3610..>3905)
                     /locus_tag="VFO88_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET9544718.1"
                     /translation="DVRLGVAGSPPASGDARLTIRPERIRIEPADTPGENRVPATIER
                     FVYLGSTTQVFLRLASGDVVQALVANAGDVEEHEVGDPVTAQLPQDALRVLES"
BASE COUNT          540 a         1408 c         1318 g          639 t
ORIGIN      
        1 ccgcgccgcc gccgcgtccg gcatgatgcg ccgcacgacg gcgacggagg gagttgatcg
       61 agcatgagca ctcgtcatcc ccggcagccg gcggggctca accgccggga gttcttgcag
      121 cggtcggccg gaggcgccat cgcgctctcc gccttcgctc cgctcctggc ggcctgcggc
      181 ggcgcggacg acaacggcgg cggcaacggc gggaacggag gaggcggcga cgacggccgg
      241 cccgagcttc agctcgcccg gccggacaac ccggtcgagt ggcagctctt cgacgacaac
      301 ccgccgatcg agtccgacct cgagccggag agcggcaccc tgcggatcta caactggaac
      361 gactacctct ggccgcgcat gctgcgccgc ttcgagaagg agtacggcgt cacggtcgag
      421 ctgacgacct tctcgaccgc cgacgaggcg accgcgaagc tcacgtcggg cgccgtcgac
      481 ttcgacgtct acttcccgac tcccgaccgg ctgggccggc tcgtcctggg gcagatcctc
      541 cagccgctca acctcgacta catcccgaac ctccagaacg tctggccctc gctccagaac
      601 ccgttctacg accagggctc gcgctacacg gtcccgtaca cgatctacac gaccgggatc
      661 ggctaccgcg ccgacgagat cgccgagccg ccggacgcgt acgggaaccc gtacgacgtc
      721 tacttcgacg agcggaacgc ggggaagatg tacctcctcg acgactcccg cgaggcgatc
      781 gcgcacatgc tcctgcgcgc cggcgtcacg gacgtgaaca ccgaggaccc ggaggcgatc
      841 gaggcggcga agcaggagct catcagcctc atcgacctcg tgaacgtgaa gctctcgacg
      901 aacgattaca cgtacatccc cgagggccgc gcgacgatcc atcaggcctg gtcggggagc
      961 ctcatctcgg cgccgtacta cctcccgcag ggcaccgggc ccgaggtgct cgggttctgg
     1021 ttcccgcagg acgggcgcgg cgtgatcggc aacgactcga tggcgatccc gaagtcggcg
     1081 cagaacccgg tgctcgcgca ccacttcctc aacttcctgc tcgacgagca gaacggctac
     1141 gacaacttcg tcaactacgt cgggtaccag ccgccgttca cgtcgatcga ccccgaccgg
     1201 ctcgtgaccg acgaggtcgt cccgccgaac ctcgagtcgg tcgtcgtgcg ggagagcgac
     1261 ttcgacgtcg ccgggttcct gctcgagctc tcgcccgagg gcgcgaacac ctggcagaac
     1321 gcctgggccg agttcaaggc aggtgtctga gccgggccga gggcgccgcc ggcgcggcgg
     1381 gctgctctgg ccgatcctcg cgctgccggg gatcgtctgg ctctgcctgt tcttcctcgt
     1441 cccgttctac gtcatcctcg gcgtcgcgtt cgggacggtc gacccgctct tcctgacgcc
     1501 cgagccggag tggaacccgg cccgctggga cccccacgcg ttcgagttcg tctgggaggg
     1561 gctcgcggcc ggcggcgggt tccggacggt cttcctgcgg acgttcgcct acgtcgcgat
     1621 cgccgtcgtg atctcgctcg cgatcgcgta cccggtcgcg tactacgtcg cccggttcgg
     1681 cgggcgcttc aaggggctcc tgctcgccgg cctcgtcgcg ccgttcttca tctcgtacct
     1741 catgcggatg ctcgcctgga tcaacctcct gcaggaggac ggctgggtga actcggtgct
     1801 cgtctgggcg cacgtgctcg acgcgccgcg gacgtggctc gacgggcggg cgtcgagcgt
     1861 gatcctcggc ctcgtctacg gctacgtgcc gttcatgatc ctgccgctct acgcgttcct
     1921 cgaccgggtc gaccggagcc tgctcgaggc ggcccgagac ctcggcgcct cgcgcttcca
     1981 cgcgttccgg ctcgtgacgc tgccgctctc gtggccggcc gtcctcgcgg gctgcgtgat
     2041 catcgcgctg ccgatgttcg gcgactacta cacgcccgac ctcctctcgg gctcgccgaa
     2101 cacgtcgctc ctcgggaacc agattgacct ctacatccgc ggcggccagc aggtcccggt
     2161 cggggcggcg ctcgtcgtct gcctcatgct cctcctcgcg gtcttcatgg cctactacct
     2221 ctgggcgacc gcgcgcgctg agcggaggct cgggcgatga ggcgctggct ccgcaacgcg
     2281 tggggcaagc cgcgcttcct cgtcctcttc acgctcgcgt acttcgcgtg gtcgatcgtg
     2341 ccgatcctcg tggcggtccg cttctcgttc aacgaggggc gctcgcgctc gaccgcgcag
     2401 ggctggtcga cgcgctggta ctgggacgac ccggatctct cggtgcgcgc ggatccggac
     2461 ctcttcggcg cgctcacgca gagcctcacg ctcgcggcga tcgccgtcgt cgtgacggtg
     2521 cccctgggcg tggcgctcgc ggtcggcctg acgcgctggc gcggccggct ggcgacggcg
     2581 tcacgcttcg gctcgatggt gccgctcgtg acgcccgaga tcgtcatggg cgtggcgctc
     2641 ctgctcgtct tcgtgcacgt gttcgagttc gtgccgctcg ggacggaggc gcaggcgatc
     2701 gggcatgtga ccttctcgct cgcgttcgtg ctgatcatcg tgcgcgggcg gctgctctcg
     2761 atcggcccgg agtacgagga ggcggcccgc gacctcggcg cgtcgcccct gcagtcgctg
     2821 cggctcgccc tgttcccgct gctcgtgccg gcgatcgtgg cgagcgcgat catcgtcttc
     2881 gccatctcga tggacgactt cgtcgtctcg aactacctgt cggcgggcgc cgcgacggac
     2941 acggtgcccg tgaagatcta ctcgagcggc cgcacggcgc cgaccccggc cctgaacgcg
     3001 ctcgcgtcgc tcacgctcgg cgtgacgatc gcgatcgcgc tcgtgctctt cgtcgtgtgg
     3061 cggctcgtcc ggcgccgccg cggcgccgaa gagccgaccg ccgaagcctt cgcggcctaa
     3121 gagcacccct cacgaccggg ctgtcgcaat cgcgctcgcg ccggccgccg ttccgccgtg
     3181 aaccatgtaa ggtcgcgcac atgcggaggc gcttcgtgac tgcgcttgca gttctggcaa
     3241 gcggctcagt tctcgtagcg ggttgcgggg cgagcgacga tcaagcagac gcagaccgct
     3301 cgaccgcttc cgtcgcaacc agctccatag cccgcgctcc cgaaagggcc gcgacgggca
     3361 agccgagcaa gtgggtctcg atcgaggact gcgaagccgt cgaggagata cttgcgaccg
     3421 tagaagcggg ccgcctgccc gacatacgcg aacagctcgt cagcctaaga ctcgagagat
     3481 cttcgaagat tcgcctgacg gaagacgggt tgctcgcttg gctagggggc agcgggatcg
     3541 ggaacggcac ttgtacgttc gattgagcca accgaccgag gctcggtcac aacccgacat
     3601 cgccgagcgc taggactcga gcacgcggag cgcgtcctgc gggagctgag ccgtcaccgg
     3661 gtcgccgacc tcgtgctcct cgacgtcgcc ggcgttcgcg acgagcgcct gcacgacgtc
     3721 gccgctcgcc aggcggagga agacctgcgt ggtcgagccg aggtagacga agcgctcgat
     3781 cgtcgcgggc acccggttct cgccgggcgt gtcggcgggc tcgatccgga tccgctctgg
     3841 gcggatcgtc aggcgcgcgt cgcccgacgc cggcggcgac cccgcaacgc ccaggcgcac
     3901 gtcgc
//
LOCUS       DASTFC010000135         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_134324,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000135 DASTFC010000000
VERSION     DASTFC010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134324"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFO88_04010"
     CDS             <1..429
                     /locus_tag="VFO88_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET9544719.1"
                     /translation="AAAVVVAGAPLPRRLALERRAPLRARGTRLVTAAAAGVAFAIAW
                     TPCVGPTLGAVLTLAASSGSAGSGAVLLGAYALGLGLPFLAAALLVTRSFAVSAWLRR
                     RRRTVQLVSAALLAVFGVLVLTGHLVDLTRELARFTGWQL"
     gene            complement(470..1483)
                     /locus_tag="VFO88_04015"
     CDS             complement(470..1483)
                     /locus_tag="VFO88_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HET9544720.1"
                     /translation="MITFGVTVLPDPPYTRLLELMELAEQSGFDNGWTYDSHILWHES
                     YPLLALAAARTQTLKLGHCVTNPGIRDPTITASYYATLHEISNGRAVMGIGRGDSSRR
                     VVGLKPVKVAEFERSLRMIKDLMNGRPVEWNGKDLQLEWVREELPPIEVWVAGYGPRA
                     LAVAGRVGDGVIIQLADPEIIKWIMDTARQAAEEDGRDPDALKCHVCAPSHISDDLAD
                     ARNQVRWFPAMVSNHVMDLIERYGFESEIPAALTEYVKARKFYDYKDHSRVGAAHGEF
                     VSDEICDRFCVLGTPEQATQKLRELESIGVDHFNIYLMTHGQEETLAAYRDSILPSFK
                     TAA"
     gene            1538..1984
                     /locus_tag="VFO88_04020"
     CDS             1538..1984
                     /locus_tag="VFO88_04020"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9544721.1"
                     /translation="MAIWGYLAFAFAAGAMLPLQFGINAELARWVDSPLRAALVSFAV
                     GTAVLLAAAVPVMRGWPELERLGAAPWWVWVGGALGAFYVLGSIVTAPRLGAATLVAL
                     ILAGQAVASLLVDHFGWVGFEEHPVTPGRLLGIALLAAGVLLVRAA"
     gene            1989..2441
                     /locus_tag="VFO88_04025"
     CDS             1989..2441
                     /locus_tag="VFO88_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET9544722.1"
                     /translation="MRPRRNPHALADAERQEAVDLLGAHMVSGAISPEDLAERTELVL
                     RARTREELHRATEGLPALPRRPALVRLAERIPLRTHVIVFVAVNVALVVVWALTRDAF
                     TSRSDEGFGLLWPFWVTLGWGVLLVAHALYTLRQPLLLRARRRGRRPR"
     gene            2459..>2724
                     /locus_tag="VFO88_04030"
     CDS             2459..>2724
                     /locus_tag="VFO88_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544723.1"
                     /translation="MTRAALVLSATAALVAGADLVHKALAVDAATLVHERGSGYLLLA
                     GVAAAWALALVAVGSPGLAFAGGVLLGGAAGNLLSLPVWGGVPNP"
BASE COUNT          295 a         1009 c          974 g          446 t
ORIGIN      
        1 gccgccgcag tcgtcgtcgc cggcgcgccg ctcccgcggc ggctcgcgct cgagcggcgg
       61 gcgccgcttc gcgcgcgcgg cacgcgcctc gtcacggcgg ccgcggccgg ggtcgcgttc
      121 gcgatcgcgt ggacgccgtg cgtggggccg acgctcggcg ccgtcctcac gctcgccgcg
      181 agctccggga gcgccggctc gggcgccgtg ctgctcggcg cctacgcgct cgggctcggg
      241 ctcccgttcc tcgccgcggc gctcctcgtc acgcgctcgt tcgcggtgtc cgcctggctt
      301 cggcgccgcc gccgcaccgt ccagctggtg agcgccgcgc tcctcgccgt cttcggcgtc
      361 ctcgtcctga ccggccacct cgtggacctg acgagggagc tggcgcgctt caccggctgg
      421 cagctctagg agcccacccc tagtaacaca gtgttacaag cgttcggcct cacgcggccg
      481 tcttgaagct cgggagaatc gagtcgcggt aggcggcgag tgtctcctcc tggccgtggg
      541 tcatgaggta gatgttgaag tggtcgacgc cgatcgactc gagctcccga agcttctgcg
      601 tcgcctgctc gggcgtgccg aggacgcaga accggtcgca gatctcgtcc gagacgaact
      661 cgccgtgcgc cgcgccgacg cgggagtggt ccttgtagtc gtagaacttc cgcgccttca
      721 cgtactcggt gagcgcggcc gggatctcgg actcgaagcc gtagcgctcg atcaggtcca
      781 tgacgtggtt cgagaccatc gccgggaacc agcgcacctg gttccgcgcg tccgcgaggt
      841 cgtccgatat gtggctcggg gcgcagacgt ggcacttgag cgcgtccggg tcgcggccgt
      901 cctcctcggc ggcctgccgc gctgtgtcca tgatccactt gatgatctcc gggtcggcga
      961 gctggatgat cacgccgtcg ccgacgcgcc ccgcgaccgc gagcgcgcgc ggcccgtagc
     1021 cggccaccca cacctcgatc ggcggcagct cctcgcgcac ccactcaagc tgaaggtctt
     1081 tcccgttcca ctcgaccggg cggccgttca tgaggtcctt gatcatccgg agcgagcgct
     1141 cgaactcggc gaccttcacc ggcttgaggc cgacgacacg ccgcgacgag tcgccgcggc
     1201 cgatccccat gaccgcgcgg ccgttcgaga tctcgtggag cgtcgcgtag tagctcgcgg
     1261 tgatcgtcgg gtcgcggatg cccgggttcg tgacgcagtg gccgagcttg agcgtctgcg
     1321 tgcgcgccgc tgcgagcgcg agcagggggt acgactcgtg ccagaggatg tgcgagtcgt
     1381 acgtccagcc gttgtcgaag cctgactgct ccgcgagctc catgagctcg agcagccgcg
     1441 tgtagggcgg atctggaagg acggtgacgc cgaacgtgat catgaacccc ctctttctgt
     1501 ggaagggaag acccaatctt ggcccgaatc gagcagcatg gcaatctggg gctacctggc
     1561 gttcgcgttc gcggcgggcg cgatgctccc tctccagttc gggatcaacg cggagctcgc
     1621 ccgttgggtg gacagcccgt tgcgcgcggc gctcgtctcg ttcgccgtcg ggacggccgt
     1681 gctgcttgcc gcagccgtgc cggtcatgcg cggctggccg gagctcgagc ggctcggcgc
     1741 ggcgccctgg tgggtgtggg tcggaggcgc gctcggcgcc ttctacgtcc tcggctcgat
     1801 cgtgacagcg ccgcggctcg gggcggcgac gctcgtcgcg ctcatcctcg ccggacaggc
     1861 cgtcgcctcg ctcctcgtcg accacttcgg ctgggtcggc ttcgaggagc atcccgtcac
     1921 gccggggcgg ctgctcggca tcgccctgct cgccgccggc gtgctcctcg tgagggcggc
     1981 ctagcgtagt gcgcccccgc cggaatccgc acgcgctcgc cgatgccgag cgccaggagg
     2041 ccgtcgacct gctcggcgcg cacatggtga gcggcgcgat ctcgcccgag gacctcgcgg
     2101 agcggaccga gctcgtgctc cgcgcccgca cgcgggagga gctccaccgg gcgaccgagg
     2161 ggctgccggc gctcccgcgg cggccggcgc tcgtccgcct cgccgagcgc atcccgctcc
     2221 gcacccacgt gatcgtgttc gtcgccgtga acgtcgcgct cgtggtcgtc tgggccctga
     2281 cgcgggatgc gttcacctcg cgaagcgacg agggcttcgg cctcctctgg ccgttctggg
     2341 tcacgctcgg gtggggcgtc ctcctcgtcg cgcacgcgct ctacacgctc cgccagccgc
     2401 tgctcctccg cgcccgccgg cgcggccgcc ggccccgctg acgcggcaga atgagggcgt
     2461 gacccgcgcc gccctcgtcc tctcggccac cgcggcgctc gtcgccggcg ccgatctcgt
     2521 ccacaaggcg ctcgcggtcg acgcggcgac gctcgtgcac gagcgcggct cgggctacct
     2581 gctcctcgcc ggagtcgcgg cggcgtgggc gctcgcgctc gtcgccgtcg ggtcgcccgg
     2641 gctcgcgttc gcgggcggcg tccttctcgg cggggcggcg gggaacctcc tctcgctccc
     2701 ggtctggggc ggcgtcccga accc
//
LOCUS       DASTFC010000136         9962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1254899,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000136 DASTFC010000000
VERSION     DASTFC010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254899"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFO88_04035"
     CDS             <1..359
                     /locus_tag="VFO88_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544724.1"
                     /translation="LAATSPATVPKILDKLREYQSAGWGGPIFWYAWRSIDSYSFVDE
                     NMNPRPAYAVFRGYLASVFAGDASRTFDARAGADGAYSAALTGLEPGTTYWVRAYASD
                     GSRTRYGSVWRFRTAH"
     gene            399..695
                     /locus_tag="VFO88_04040"
     CDS             399..695
                     /locus_tag="VFO88_04040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9544725.1"
                     /translation="MDGDLRVIDRPAELAYRLYVGEREAGELRYARQGDRLVLLHTEV
                     DRSLGRQGLGSALVRGALDDVRARGLEIVPRCPFVRRYLERHPEYTDLVADPES"
     gene            complement(712..1977)
                     /locus_tag="VFO88_04045"
     CDS             complement(712..1977)
                     /locus_tag="VFO88_04045"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9544726.1"
                     /translation="MLEAVRSWSGETLVFRAATAVALVHALDDSFLGRQPGVPLGQHA
                     LAAALALVLGGAAIVWFPRLRPVLAALVSLVFGVLAIGNGALHLIHVALDGPAASDLT
                     GIAAFAAGIVLVLLAPAILFRHRGERERSARRRWTLRVLAAGGALLLAYALVFPLTVA
                     VVQTHKFREPIGEPPSAAYEPVSFEAEDGLELSGWYVPSENGAAVVVVHGGGGDRMGT
                     LDHAELLARNGYGVLVYDSRGRGESEGSHNAFGWGWEKDVAGALDFLRARADVDPDRI
                     GGLGLSTGADVLIEVAPERRDLRAVVSDGATARSFDDYLSLAGLDEGAPFFWTMLTGA
                     RVFSGSSPGEPLEDLAAQVSPTPLLLVSTGLGVQNELGFNRVYSEAAREPVELWELPD
                     VRHTAAVRERPDEYEERVIGFLDEALAQR"
     gene            complement(1977..4532)
                     /locus_tag="VFO88_04050"
     CDS             complement(1977..4532)
                     /locus_tag="VFO88_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9544727.1"
                     /translation="MRRVALRGLASRKLRSLLTAFAIVLGVAMVSGTFVLTDTIDSGF
                     DTVFSESYEGADAVISPETEFGRQAAGSGGFPASVLEDVEALPSVAEAAGSVRDSARL
                     IGRDGEPLGGSSVSPLALGLDPADEQFSPVRIVDGEWPDGSGQIAIDRRTFDDEGLAL
                     GDAISATARGPVQEFEIVGVVELGSLESLGGATISVFDLATAQELFAKEDVVDQIQLA
                     AAEGVSAEALVAEVRGELPATLDVQTTAAQVETESEDTSAGLGFLRYFLLAFAAIALF
                     VGSFVIANTLSITIGQRTRELATVRTLGASRRQVLWSVVLEALVVGVLASIVGILLGV
                     GLALGLNELLAAAGIELPTSGLVLETRTIVVGLVVGIGITLLASIRPALRATRVPPIA
                     AVREGSVLPPSRFARFGLPASLTLTAGAVALLLVGSLGDGLSAAPRLLAIGLGVILLF
                     VGIALVAPRLVRPLASALGWPGTRIAGAAGRLARANAMRNPARTASTASALMIGLALV
                     TLVAILAQGLRSTFVDSVDELFVADYAVVAEGGFNPLTPEVEEALAQAPGVEAVSGVR
                     SGSGRIAGEDVGVSAVPGNIAETVAIRWDEGSDAVPGALGEDGAFVTREFADERDLEL
                     GSQLEIETPTGETLALAVEGIWEEPKGGSPFGQVAVSHEAFDPVFPEPRNDFTFVNVA
                     GGITADAEAGLETALADFPDAKVQTRDEFKDESVASLSRVLNILYVLLALSVVVSLFG
                     LVNTLVLTVFERTRELGMLRAVGMTRRQVRRMIRHESIVTALLGATLGIAIGVFLAAL
                     VTQALGDEGFSFALPIGSLVVFVLAAILVGVLAAIMPARRAARLDILEALRYE"
     gene            complement(4529..5245)
                     /locus_tag="VFO88_04055"
     CDS             complement(4529..5245)
                     /locus_tag="VFO88_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9544728.1"
                     /translation="MAARDLARRYGEGDNAVDALRGVSLAIEEGQLTAVMGPSGSGKS
                     TLMHLLAGLDRPTEGEVWIDSTEIGTLGDNDLTRLRRRHIGFIFQFFNLLPMLSAEEN
                     IVLPLELAGGRTDRAWVDELIDRVGLADRRDHRPSELSGGQQQRVAIARALASKPTVV
                     FADEPTGNLDSATSGEILELLRTSVDAYGQTTVMVTHDANAAAVADRVLFLADGRIVK
                     EIGRCSPHDVLLALEEVAQA"
     gene            5521..6651
                     /locus_tag="VFO88_04060"
     CDS             5521..6651
                     /locus_tag="VFO88_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9544729.1"
                     /translation="MRLRSLLAGRAVDAFLVALAVLAQIEAWVDPSQAPRAVTVPATL
                     LWSVPLLFRRRFPLGAPLTVFGVLAAEAIAAGDAVTSSQVNAVALLAAFGVLGAHREL
                     RLALAGAAVGYAALVTIIVNDDLAAEGAVPVLVFAAVAWAIGRTIGEHGRRAEVLAER
                     ADRLERANAEAVAGERARIARELHDVIAHSVSVMTVQAGAARLLLDEDPDRARAPLLA
                     VEETGREALAEMRRLLGILRGDDERAALAPQPSIARIDALAEQVRTAGLPVDVHVEGE
                     PRPLSPGVDLAAYRVVQEALTNALKHAGAARARVDLCYRDTALELAIMDDGRAPANGA
                     GAGHGIVGMRERVSLYGGELRAGPRREGGFEVRATLPLEASE"
     gene            6648..7328
                     /locus_tag="VFO88_04065"
     CDS             6648..7328
                     /locus_tag="VFO88_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544730.1"
                     /translation="MTIRVLVADDQALVRGGFRMILESQKDIEVVGEAGTGREALARA
                     RELDPDVILMDIRMPELDGLEATRRLLAGAGGTRPRVLVVTTFDADEYVYAAMKAGAS
                     GFVLKDLRPEQLADAVRVVAAGESLLAPAITRRLVEQFVRRPPPGSARPPELETLTER
                     ELGVLRLVARGRSNAEIAQELVLSEATVKTHLTHILTKLRLRDRVQAVVVAYESGLVQ
                     PGEGADDG"
     gene            7400..8887
                     /locus_tag="VFO88_04070"
     CDS             7400..8887
                     /locus_tag="VFO88_04070"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9544731.1"
                     /translation="MPEQLFIGGEYVDSTSDRVIEVENPATEEVIAEVPDASAEDVDR
                     AIQAARKAQREWRKTDGLERANLLHECARRMDEAGDELAVLLTHEGGKTLKENRDEVE
                     WCGTAIRHLAEIARAQQGRVVGPTKPGQMNLILNEPVGVVGHIFPFNYPLVLLAWQVP
                     AALATGNTCIVKPAVQTPLATLKLGEIFEPLPAGTFNVLTAGAEGSAALVEHRGTDMI
                     AFTGSIATGSKVMAAAAPRIKKLLLELGGSDPFIVVGDADVDTAAAGAVFAAFLNAGQ
                     VCTSAERFFIDESIYDTFMAKAMELARELRIGDPMEDVDLGAMVSEDARSGAEQAIEE
                     LVSRGASVLAGGGAPSHLDRGYFYEPTFVELSDPRSQRLDREVFGPLATFTRVRDLDE
                     AIELANDSDYGLGANIYTSDLETAMRAATELEAGMVWINDPLKDNDAAPFGGHKMSGL
                     GRELGTEGISAFTQAKHVHIDFAQQPSPEWWFPYERPKIATEVEV"
     gene            8884..>9962
                     /locus_tag="VFO88_04075"
     CDS             8884..>9962
                     /locus_tag="VFO88_04075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9544732.1"
                     /translation="MSGRSLWLDEALVADAGGAAPALEGDVKADVCIVGGGYVGLWTA
                     LELRERDPGIDVVVVEADVCGGGASGRNGGFVLSWWAKFKTLRALLGDEEALRVAEAS
                     ADAVTEIGAFCEREGIDAHFRPDGWLWTATSDAQRGAWKATLAALDAVGASPFEELSP
                     EEAARRGGSSMHVAGVLEATAATVQPALLARGLRRVALDRGVRIFERSRMTGLEPWGV
                     RVEAGRVAAERVVLATNAWAAGVRELRRSLVVIASDMVATEPFGERLARDGWADGVAI
                     SDSRLLVNYFRSTLDGRVAFGRGGGTLAFSGRVGSAFDGESPRADDVAAEWRRLYPQF
                     ADVPIAASWTGPIDRSVTSLPFFGRL"
BASE COUNT         1371 a         3588 c         3729 g         1274 t
ORIGIN      
        1 cgctcgccgc gacgtcgccc gcgacggtcc cgaagatcct cgacaagctc cgcgagtacc
       61 agtcggcggg ctggggcggg ccgatcttct ggtacgcgtg gcgctcgatc gactcgtact
      121 cgttcgtcga cgagaacatg aaccccaggc ccgcgtacgc ggtcttccga ggctaccttg
      181 cctccgtctt cgcaggcgac gcctcccgca cgttcgacgc ccgggccggc gcagacggcg
      241 cgtactcggc ggcgctgacc gggctcgagc ccgggacgac ctactgggtt cgcgcttacg
      301 cgagcgacgg aagccgcacg agatacggga gcgtctggag gttccgaacg gcccattgag
      361 caggtgccgt ccgggcggtg accggatacc gtgatcgtgt ggacggcgat ctgcgcgtga
      421 tcgaccggcc ggccgagctc gcctaccggc tgtacgtggg cgagcgggag gccggcgagc
      481 tccggtacgc ccggcaaggg gaccggctcg tgctcctcca cacggaggtc gaccggtcgc
      541 tcgggcgaca ggggctcggg tcggcgctcg tacggggcgc gctcgacgac gtccgcgcgc
      601 gcgggctcga gatcgtcccg cgatgcccct tcgtgcgccg ctacctcgag cggcacccgg
      661 agtacaccga cctcgtcgcg gatcccgagt cgtaatcgcg tcgccgcggc ctcagcgctg
      721 cgcgagcgcc tcgtcgagga acccgatcac gcgctcctcg tattcgtcgg ggcgctcccg
      781 cacggcggcg gtgtgccgca cgtcagggag ctcccagagc tcgacgggct cccgggcggc
      841 ctctgagtag acccggttga acccgagctc gttctggaca cccaggccgg tcgagacgag
      901 caggagcggg gtcggggaga cctgcgcggc caggtcctcg agcggctcgc cgggcgaaga
      961 gccggagaag acgcgtgcgc cggtcagcat cgtccagaag aacggtgccc cttcgtcgag
     1021 gcccgctagg gataggtagt cgtcgaacga ccgggcggta gctccgtcgg agacgaccgc
     1081 acgcaggtcg cgacgctcgg gtgcgacctc gatgaggacg tcggccccgg tcgagagccc
     1141 gagcccgccg atccgatcgg ggtcgacgtc cgcccgcgcg cggaggaagt cgagcgcgcc
     1201 ggccacgtcc ttctcccagc cccagccgaa cgcgttgtgg ctgccctcgc tctccccgcg
     1261 gccgcgcgag tcgtagacga gcacgccgta tccgtttcgt gcgagcagct cggcgtggtc
     1321 gagcgtgccc atccgatcgc cgccgccgcc gtgcacgacg acgaccgcgg cgccgttctc
     1381 cgacggcacg taccacccgg acagctcgag cccgtcctcg gcctcgaacg agacgggctc
     1441 gtaggccgcg gagggaggct cgccgatcgg ctcgcggaac ttgtgcgtct gcacgaccgc
     1501 gaccgtcaag gggaagacca gggcgtaggc gaggaggagg gcgccgccgg ccgcgagcac
     1561 gcgcagcgtc cagcggcgcc gcgccgaccg ttcccgctcg ccgcggtggc ggaagaggat
     1621 cgccggcgcg aggaggacga gcacgatccc ggcggcgaag gcggcgatcc ccgtcaggtc
     1681 gctcgccgca ggcccgtcga gcgccacgtg gatcaggtgg agcgcgccgt tcccgatcgc
     1741 gagcacgccg aagacgagcg agacgagcgc cgcgagcacg ggccgcagcc gcgggaacca
     1801 cacgatcgcc gcgccgccga ggacgagcgc gagcgcggcc gccagcgcgt gctgcccgag
     1861 gggcacgccc ggctggcggc cgaggaacga gtcgtcgagc gcgtgcacga gcgcgaccgc
     1921 ggtggccgcc cggaagacga gcgtctcccc gctccagctc cggacggcct caagcatcac
     1981 tcgtaccgaa gcgcctcgag gatgtcgagc cgagccgccc gccgcgccgg catgatcgcc
     2041 gcgagcacgc cgacgaggat cgccgcgagg acgaacacga cgagcgaccc gatcggcagc
     2101 gcgaacgaga agccctcgtc gccgagcgcc tgcgtcacga gcgccgcgag gaagacgccg
     2161 atcgcgatcc cgagcgtcgc acccaggagc gccgtgacga tgctctcgtg ccggatcatc
     2221 cgccgcacct ggcgccgcgt catcccgacg gcgcgcagca tcccgagctc gcgcgtccgc
     2281 tcgaacaccg tgaggacgag cgtgttcacg agcccgaaga ggctcacgac gacggagagg
     2341 gcgaggagga cgtagaggat gttcagcacc cggctcagcg aggcgacgct ctcgtccttg
     2401 aactcgtccc gcgtctgcac cttcgcgtcg gggaagtcgg cgagcgcggt ctcgaggccg
     2461 gcttccgcgt ccgccgtgat cccgcccgcg acgttcacga acgtgaagtc gttgcgcggc
     2521 tccgggaaga cggggtcgaa cgcctcgtgc gagaccgcca cctgcccgaa cggcgagccg
     2581 cccttcggct cctcccagat cccctcgacc gcgagcgcga gcgtctcgcc cgtcggggtc
     2641 tcgatctcga gctgcgagcc gagctcgagg tcgcgctcgt cggcgaactc gcgcgtgacg
     2701 aaggcgccgt cctcgccgag cgcgccggga acggcatccg agccctcgtc ccaccggatc
     2761 gcgacggtct cggcgatgtt ccccggcacg gcggagacgc cgacgtcctc gcccgcgatc
     2821 cgccccgagc cgctccgcac gccggagacc gcctcgacgc ccggcgcctg cgcgagcgcc
     2881 tcctcgacct cgggggtgag cggattgaag ccgccctcgg ccaccacggc gtagtcggcg
     2941 acgaagagct cgtcgacgga gtccacgaac gtcgagcgga gcccctgcgc gaggatcgcc
     3001 acgagcgtga cgagcgcgag gccgatcatg agcgccgacg cggtcgacgc ggtgcgcgcc
     3061 gggttccgca tggcgttcgc gcgggcgagg cgaccggccg cgcccgcgat ccgcgtcccc
     3121 ggccagccga gcgccgaggc gagcggccgc acgaggcgcg gcgcgacgag cgcgatcccg
     3181 acgaagagga ggatcacgcc gagcccgatc gcgaggagcc gcggagcggc cgagagcccg
     3241 tcgccgaggc tccccaccag gagcagcgcg accgcgccgg cggtcagcgt gagcgaggcg
     3301 gggagcccga agcgggcgaa gcgcgacggc gggagcacgg agccctcgcg gacggccgcg
     3361 atcggcggta cccgcgtcgc ccggagcgcc ggccggatgc tcgcgagcag cgtgatcccg
     3421 atcccgacga cgagcccgac gacgatcgtg cgcgtctcga gcacgagccc gctcgtcggc
     3481 agctcgatcc cggccgcggc gaggagctcg ttcagcccga gcgcgagccc gaccccgagc
     3541 aggatcccca cgatcgacgc gagcaccccg acgacgagcg cctcgaggac gaccgaccag
     3601 agcacctgcc ggcgcgaggc gcccagcgtc ctcacggtgg cgagctcacg cgtgcgctgg
     3661 ccgatcgtga tcgagagggt gttcgcgatc acgaagctcc cgacgaacag ggcgatcgcc
     3721 gcgaacgcga gcaggaaata gcgcaggaac ccgagccctg cgctcgtgtc ctcgctctcc
     3781 gtctcgacct gcgccgccgt tgtctggacg tcgagcgtcg ccggcagctc gccgcggacc
     3841 tcggcgacga gcgcctccgc ggagacgccc tcggccgccg cgagctggat ctggtcgacg
     3901 acgtcctcct tcgcgaagag ctcctgcgcc gtcgcgaggt cgaacaccga gatcgtcgcg
     3961 ccgccgagcg actcgagcga gccgagctcg accacgccga cgatctcgaa ctcctgcacg
     4021 ggcccgcggg cggtcgcgct gatcgcgtcg ccgagcgcga gcccctcatc gtcgaacgtc
     4081 cggcggtcga tcgcgatctg gccggagccg tccggccact cgccgtccac gatccggacc
     4141 gggctgaact gctcgtcggc cgggtcgagc ccgagcgcga gcggcgagac gctcgacccg
     4201 cccagcggct cgccgtcgcg gccgatcagc cgcgcggagt cgcggacgga gccggccgcc
     4261 tcggcgaccg agggcagcgc ctcgacgtcc tcgagcacgg aggcggggaa cccgccgctg
     4321 ccggccgcct ggcgcccgaa ctcggtctcc ggggagatga cggcgtcggc gccctcgtac
     4381 gactcgctga agacggtgtc gaagccgctg tcgatcgtgt ccgtcaggac gaaggtgccg
     4441 ctcaccatcg caacgccgag gacgatcgcg aacgccgtga ggagcgagcg gagcttgcgg
     4501 gaggcgaggc ctctgagggc gacgcgcctc atgcctgtgc cacctcctcg agcgcgagga
     4561 gcacgtcgtg cgggctgcag cggccgatct ccttcacgat ccgcccgtcg gcgagaaaga
     4621 gcacccggtc ggcgaccgcg gcggcgttcg cgtcgtgggt gaccatcacg gtcgtctgcc
     4681 cgtaggcgtc gacggacgtc cggagcagct cgaggatctc gccgctcgtc gccgagtcca
     4741 ggttgccggt cggctcgtcc gcgaagacga ccgtcggctt ggaggcgagc gcccgcgcga
     4801 tcgcgacccg ctgctgctgg ccgcccgaga gctcggacgg ccggtggtcg cgccggtcgg
     4861 caaggccgac gcggtcgatc agctcgtcca cccaggcgcg gtcggtgcgc ccgccggcga
     4921 gctcgagcgg gaggacgatg ttctcctccg cgctcagcat cgggagcagg ttgaagaact
     4981 ggaagatgaa gccgatgtgc cgtcggcgga gcctcgtcag gtcgttgtcg ccgagggtgc
     5041 cgatctccgt cgagtcgatc cagacctcgc cctcggtcgg ccggtcgagc ccggcgagca
     5101 ggtgcatgag cgtcgacttg ccggaaccgg aggggcccat gacggccgtg agctggccct
     5161 cctcgatcgc gagcgagacg ccgcgaagcg cgtcgacggc gttgtcgccc tcgccgtagc
     5221 ggcgggcgag gtcgcgggcc gccacacgaa cgggccggcc gtttgcggcg ctctccgcgg
     5281 ggcttggagt ggtggcggag gtctccatgt gtcctttcgg tcgtcgttcg agtcgcagcc
     5341 gaggctaggc ggcaccgccc gccgcaacgt cggcgccgcg tcggatccgg gggcgccgcc
     5401 gggatgaacc tcgccgcgcc cgtctcggac cgggggctga gcggcctacg accgcagtcg
     5461 gatcgggatc ggcgccgcgg ccgaggcggg cgggctcgca cgcggactac cgttcgtccc
     5521 atgcgtcttc gctcgctgct cgccggccgt gccgtcgacg cgttcctcgt cgccctcgcc
     5581 gtcctcgcgc agatcgaggc ctgggtcgac ccgagccagg cgccccgcgc ggtcacggtg
     5641 cctgccacgc tcctctggtc ggttccgctt ctcttccggc gccgtttccc gctcggcgcg
     5701 ccgctcacgg tgttcggcgt gctcgccgcg gaggcgatcg cagccggcga tgccgtcacc
     5761 tcgtcgcagg tgaacgcggt cgcgctcctc gcggcgttcg gggtgctcgg cgcccaccgc
     5821 gagctcaggc tggcgctcgc gggcgccgcc gtcggctacg cggcgctcgt gacgatcatc
     5881 gtgaacgacg acctcgcggc cgagggcgcc gttccggttc tcgtcttcgc ggcggtcgcg
     5941 tgggcgatcg gcaggacgat cggcgagcac ggccgccggg ccgaggtcct cgcggagcgc
     6001 gccgaccggc tcgagcgagc gaacgcggag gcggtcgccg gcgagcgggc acggatcgcg
     6061 cgcgagctcc acgacgtgat cgcgcacagc gtgagcgtga tgaccgtcca ggcgggcgcc
     6121 gcgaggctcc tgctcgacga ggacccggac cgcgcgcgcg cgccgcttct cgcggtcgag
     6181 gagaccgggc gcgaggccct cgccgagatg cggcgcctgc tcggcatcct ccggggcgac
     6241 gacgagcgcg cggcgctcgc gccccagccg agcatcgccc gaatcgacgc gctcgcggaa
     6301 caggttcgca cggccggcct gccggtcgac gtgcacgtcg agggcgagcc gcgaccgctc
     6361 tcgccgggcg tcgacctcgc ggcgtaccgc gtcgtccagg aggcgctcac gaacgccctg
     6421 aagcacgccg gggcggcgcg ggcgcgcgtc gacctgtgct accgggacac cgcgctcgag
     6481 ctcgcgatca tggacgacgg ccgcgcgccc gcgaacggcg ccggcgccgg acacgggatc
     6541 gtcgggatgc gcgagcgcgt gtccctctac ggcggcgagc tccgggcggg gccccgccgc
     6601 gagggcgggt tcgaagtgcg cgcgacgctt ccgctcgagg cgtccgagtg accatccgcg
     6661 tcctcgtcgc cgacgaccag gcgctcgtgc gaggcggctt ccggatgatc ctcgagtccc
     6721 agaaggacat cgaggtcgtc ggcgaggccg gcaccggtcg cgaggcgctc gcccgcgctc
     6781 gggagctcga cccggacgtg atcctcatgg acatccggat gcccgagctc gacggcctgg
     6841 aggcgacgcg gcgcctgctc gccggcgccg gcggtacccg gccgcgcgtg ctcgtggtca
     6901 cgaccttcga cgcggacgag tacgtctacg cggcgatgaa ggcgggcgcg agcgggttcg
     6961 tcctcaagga tctccgcccc gagcagctcg ccgacgccgt tcgcgtcgtc gcggccggcg
     7021 agtcgctcct cgcgccggcg atcacccgtc ggctggtcga gcagttcgtc cggcgcccgc
     7081 cgcccggctc ggcccgcccg cccgagctcg agacgctgac ggagcgggag ctcggcgtcc
     7141 tgcgcctcgt cgcccgcggc cgctcgaacg cggagatcgc ccaggagctc gtcctcagcg
     7201 aggcgacggt gaagacgcac ctgacgcaca tcctcacgaa gctgcggctc cgcgaccgcg
     7261 tccaggcggt cgtcgtcgcg tacgagtcgg gcctcgtcca gcccggcgag ggcgccgacg
     7321 acggctgacc tttcgcgcgg gctcccggtc gcctagcctt cgcggtccaa gcggcaagcg
     7381 agcaagcgga ggggaagcca tgcccgagca gctgttcatc ggcggggagt acgtcgactc
     7441 gacgtccgac cgtgtgatcg aggtggagaa cccggcgacg gaggaggtca tcgccgaggt
     7501 gccggacgcg tccgcggagg acgtcgaccg cgcgatccag gcggccagga aggcgcagcg
     7561 cgaatggcgc aagacggacg gcctggagcg cgcgaacctc ctgcacgagt gcgcgcgccg
     7621 tatggacgag gcgggcgacg agctcgccgt gctcctcacg cacgagggcg gcaagacgct
     7681 gaaggagaac cgcgacgagg tcgagtggtg cggcaccgcg atccgccacc tcgccgagat
     7741 cgcgcgcgcc cagcagggcc gtgtcgtcgg cccgacgaag ccgggccaga tgaacctgat
     7801 cctgaacgag ccggtcggcg tcgtcgggca catcttcccg ttcaactatc cgctcgtcct
     7861 gctcgcgtgg caggtgcccg cggcgctcgc gaccgggaac acgtgcatcg tcaagccggc
     7921 cgtccagacg ccgctcgcga cgctcaagct gggcgagatc ttcgagccgc ttccggccgg
     7981 gacgttcaac gtgctgacgg cgggcgcgga gggctcggcg gcgctcgtcg agcaccgcgg
     8041 gacggacatg atcgccttca ccggctcgat cgcgaccggg tcgaaggtga tggcggcggc
     8101 ggcgccgcgg atcaagaagc tcctgctcga gctgggcggc tccgacccgt tcatcgtcgt
     8161 cggggacgcg gacgtcgaca cggccgccgc gggcgcggtc ttcgccgcgt tcctgaacgc
     8221 cggccaggtg tgcacgtcgg ccgagcggtt cttcatcgac gagtcgatct acgacacgtt
     8281 catggcgaag gcgatggagc ttgcgaggga gctgcggatc ggcgatccga tggaggacgt
     8341 cgacctcggc gcgatggtct ccgaggacgc gcgctcgggc gcggagcagg cgatcgagga
     8401 gctcgtctcg cgcggggcgt cggtgctcgc gggcggtggg gcgccctcgc acctcgaccg
     8461 cgggtacttc tacgagccga cgttcgtcga gctctccgac ccgcgctcgc agcggctcga
     8521 ccgcgaggtc ttcgggcccc tcgccacgtt cacgcgcgtg cgcgacctgg acgaggcgat
     8581 cgagctcgcg aacgactccg actacgggct cggcgcgaac atctacacgt ccgatctcga
     8641 gacggccatg cgggcggcga ccgagctcga ggcggggatg gtctggatca acgacccgct
     8701 gaaggacaac gacgcggccc cgttcggcgg gcacaagatg agcggcctcg gccgcgagct
     8761 cgggacggag gggatcagcg ccttcacgca ggcgaagcac gtccacatcg acttcgccca
     8821 gcagccctcg cccgagtggt ggttcccgta cgagcggccg aagatcgcga cggaagtcga
     8881 ggtgtgagcg gccggagcct ctggctcgac gaggcgctcg tcgccgatgc gggcggcgct
     8941 gcgcctgcgc tcgagggcga cgtcaaggcg gacgtctgca tcgtcggcgg cggctacgtc
     9001 gggctctgga ccgcgctcga gctcagggag cgcgacccgg gaatcgacgt ggtcgtcgtc
     9061 gaggcggacg tctgcggcgg cggcgcgagc gggcgcaacg gcggcttcgt tctctcgtgg
     9121 tgggcgaagt tcaagacgct gcgcgcgctg ctcggcgacg aggaggcgtt gcgggtggcc
     9181 gaggcgtccg cggatgcggt cacggagatc ggcgcgttct gcgagcgaga ggggatcgac
     9241 gcccacttcc ggccggacgg ctggctctgg accgcgacct ccgacgccca gcgcggagcg
     9301 tggaaggcga cgctcgcggc gctcgacgcg gtcggcgcgt cgccgttcga ggagctttcg
     9361 ccggaggagg ccgcacggcg cggcggctcg tccatgcacg tggccggcgt gctcgaggcg
     9421 actgccgcga cggtgcagcc ggcgctcttg gcccgggggc tgcggcgcgt ggcgctcgac
     9481 cgcggcgtcc ggatcttcga gcggtcgcgg atgacggggc tcgagccgtg gggtgtgcgc
     9541 gtggaggcgg gccgcgtggc ggcggagcgc gtcgtgctcg cgacgaacgc gtgggcggcc
     9601 ggcgttcgcg agctgcggcg ctcgctcgtc gtgatcgcga gcgacatggt cgccacggag
     9661 ccgttcggcg agcggctcgc gcgcgacggc tgggcggacg gcgttgcgat ctcggactcc
     9721 cggctgctcg tgaactactt ccgctcgacg ctcgacgggc gcgtggcgtt cgggcgcggc
     9781 ggcgggacgc tcgcgttctc cggccgcgtc ggctccgctt tcgacggcga gtctccccgt
     9841 gcggacgatg tcgccgccga gtggcgccgg ctctacccgc agttcgccga cgtcccgatc
     9901 gcggcgagct ggacgggccc gatcgaccgc tcggtcacgt cgctcccgtt cttcggccgg
     9961 ct
//
LOCUS       DASTFC010000137         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1139661,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000137 DASTFC010000000
VERSION     DASTFC010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139661"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..777)
                     /locus_tag="VFO88_04080"
     CDS             complement(43..777)
                     /locus_tag="VFO88_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET9544733.1"
                     /translation="MTEVPARAAVLPPRDCDEREFDDWVRWATERADPFMAWLGVVFA
                     LLVGYDIAVDVSPSTSTALEVAGWTIWGVFALEFFAKLALAPAKRRFVRRHWIQVVML
                     LIPTLRVLRFLRLLRLGRALPAARVVSSSYRSVGTARRLFRSRLGYLAGASAVVAIAL
                     AELAFLFERDAPDPAFDSFGDAVLWGFTVVLALQGDPVPSSAGGRIVMLGGFVFGLVV
                     VASLAGTIGAFFVDDRRERAEREEVG"
     gene            complement(774..1352)
                     /locus_tag="VFO88_04085"
     CDS             complement(774..1352)
                     /locus_tag="VFO88_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544734.1"
                     /translation="MSTRRVLLAAAAALLLAASACGGSSEGEPETVTTETETEEASGG
                     DELAERCENPDAGYAVRYPAGWHTNDGSVTAPCSFFHPEPFELPEASEPPPLAVSISR
                     EPIALADLADESPATRVLASEEVEVAGRPALRRETESTGDGLLPAGVRSLEYLVDLDG
                     ETLVAATRSHGGLAFERNGEVLQEMVATLEQR"
     gene            complement(1349..2251)
                     /locus_tag="VFO88_04090"
     CDS             complement(1349..2251)
                     /locus_tag="VFO88_04090"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HET9544735.1"
                     /translation="MTAAALAGPAATHRYTYSIASRGDVEADLEWFRHIARTALTNER
                     GWTLGGHSRFVEVPSGGDFRLILASPAEVNAASPVCDRPWSCRVGDDVLINDYRWRHG
                     TEAWTLGYNAYRRYVVNHEVGHWLGLGHVSYCGKGNRAAVMQQQSKSLEGCEANVWPL
                     YSEWLRLGRSAYWPTPIPAWFWPWLGWYVGHADYTTPRSVATYPDAAPSPVPAWTWEF
                     LRWYLHHGEYAEDDFRDPATYPDEAPSPVPRWTWQFYKWWFGHEEYTAFRSPVTRPDA
                     APRRLPDWAWARLSVHLAATRLGL"
     gene            complement(2296..2649)
                     /locus_tag="VFO88_04095"
     CDS             complement(2296..2649)
                     /locus_tag="VFO88_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9544736.1"
                     /translation="MTIAGEPLTSTARHDGVDVVTLAGDLDLFSAEVLRRELEEAVPG
                     GRHVVVLDLADVTFLDSTVLGLLLLAARRMEAHGGRLVLASAGEAVERVLQVSGLSTY
                     LPVHDTVAEAVAAEG"
     gene            complement(2646..3194)
                     /locus_tag="VFO88_04100"
     CDS             complement(2646..3194)
                     /locus_tag="VFO88_04100"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HET9544737.1"
                     /translation="METKTRHRTGMNGSAASEERLEPVRVATVPTYDEAADAIEWLSS
                     ARFPIERATIVARDLKLVERVVGTRTRRRAAADGAGTGVVVGAGVGLLLGLLSEDVGV
                     AGTLAWGAILGAAIGALLALALHAWSERRPFESVQTLDATYYDVLVEERVAAEAVGIL
                     TEWAVKDPARRLETRLAEPELS"
     gene            complement(3279..3968)
                     /locus_tag="VFO88_04105"
     CDS             complement(3279..3968)
                     /locus_tag="VFO88_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544738.1"
                     /translation="MSARILLAEDDAGVREVVGYALEREGFAVTPVEDASAALAHARE
                     QEYDIVVLDVMLPDGSGFDVCRTLRAESSVPIVMLTAKDAEVDRVLGLELGADDYVTK
                     PFSSAELVSRLRALLRRRELDRSAAAASPLEVGGLRVDPARHEVTVDGEPIAVTPSEL
                     KILTLLASRPGRVVSRREVMQHLWQTDFVGDEHACEVHVSNLRRKVEADPANPRRILT
                     VRGAGYKLAAP"
     gene            complement(3965..>5350)
                     /locus_tag="VFO88_04110"
     CDS             complement(3965..>5350)
                     /locus_tag="VFO88_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9544739.1"
                     /translation="GTALDEALAGRRYVETAGDGAGTVVALPVRSGPASAVVAFASHP
                     ELEAGLGLARSTITEAALWGVLVGGLAGILVAALIARRLRRIAASAAAIEARDFDRPV
                     TARFPDEVGALATSLDRMRLHLREAFTELGRERDRLERLLERLHEGVVTIAPDGTVEF
                     ANEAAAELLAPARLEEGAPLPEPRLGALAAELFRPGATVAHERVVDGDRTLAVVGIPA
                     HGADTAMLVLEDVSEQERRRRAEREFVSNASHELRTPLQTILGAVEALQAGALDDPDA
                     RGRFLALIEREGRRLSRLTRALLVLARAQTGAERVELAPVRILPLLEAVAGALQAPPG
                     VRVEVACPPGLEALADADLLEQAVANLAANAAHHTEEGTIRLSAEANGDGVAIEVADT
                     GPGVPARARERLFDRFYRVEGRAADRFGLGLAIARESARALGGDVHIDAAPGGGTLAR
                     VLLRAARLEAV"
BASE COUNT          749 a         2011 c         1954 g          636 t
ORIGIN      
        1 gcaggtgccc gccgcggcgg cgggcaggca ttgcgcggcc gcctagccca cctcctcgcg
       61 ctcggcccgc tcgcgccggt cgtcgacgaa gaacgcgccg atcgtgccgg cgagcgaggc
      121 gacgaccacg aggccgaaga cgaagccacc gagcatgacg atccgcccgc ctgccgacga
      181 cgggacgggg tcgccctgga gcgcgagcac gaccgtgaac ccccagagca cggcgtcccc
      241 gaacgagtcg aacgcggggt cgggcgcgtc gcgctcgaag aggaaggcca gctcggcgag
      301 cgcgatcgcg acaaccgcag acgcgcccgc gagatagccg agccgggagc ggaagagccg
      361 ccgcgccgtc cctaccgacc ggtaggagga cgagacgacg cgcgcggccg ggagcgcacg
      421 cccgagacgc aggagccgca ggaagcggag caccctgagc gtcggaatca aaagcatcac
      481 aacctggatc cagtggcggc gcacgaaccg ccgcttcgcc ggcgcgagcg cgagcttcgc
      541 gaagaactcg agcgcgaaga cgccccagat cgtccacccg gcgacctcga gcgcggtcga
      601 ggtgctcgga ctcacgtcga ccgcgatgtc gtagccgacg agcagggcga acacgacgcc
      661 gagccaggcc atgaacggat cggcgcgctc ggtcgcccac cggacccagt cgtcgaactc
      721 gcgctcgtcg caatcgcgcg gcggaagtac cgcggcgcgc gccgggacct ccgtcatcgc
      781 tgctcgaggg tcgccaccat ctcctggagg acttccccgt tgcgctcgaa ggcgagcccg
      841 ccgtgcgagc gcgtcgccgc gacgagcgtc tcgccgtcga ggtcgacgag gtactcgagg
      901 ctgcggacgc ccgccggcaa caggccgtcg ccggtcgact cggtctcgcg gcggagcgcc
      961 gggcggcccg caacctcgac ctcctcgctc gcgaggacgc gcgtggctgg gctctcgtcc
     1021 gccaggtcgg cgagcgcgat cggctcgcgc gagatcgaga cggcgagcgg cggcggctcg
     1081 ctcgcctccg gcagctcgaa cggctccggg tggaagaacg agcacggcgc ggtgacggag
     1141 ccgtcattcg tgtgccagcc ggccggatag cggacggcgt aacccgcgtc cgggttctcg
     1201 caccgctcgg cgagctcgtc gcccccgctc gcctcctccg tttcggtctc cgtcgtcacc
     1261 gtctcgggct cgccctccga actgccgcca cacgcggacg cggcgaggag gagcgccgcc
     1321 gccgccgcga gcagcacacg ccgcgtgctc acagcccgag cctcgtcgcg gcgaggtgga
     1381 cgctcagccg tgcccacgcc cagtccggca gcctgcgcgg agcggcgtcc ggcctcgtga
     1441 ccgggctccg gaaggccgtg tactcctcgt ggccgaacca ccacttgtag aactgccacg
     1501 tccagcgcgg caccgggctc ggcgcctcgt ccggatacgt ggccgggtcg cggaagtcgt
     1561 cctccgcgta ctcgccgtgg tggaggtacc accggaggaa ctcccaggtc caggccggca
     1621 cagggctcgg cgctgcatcg gggtacgtcg cgacgctacg gggagtcgtg taatcggcgt
     1681 ggccgacgta ccacccgagc cacggccaga accacgctgg gatcggagtc ggccagtagg
     1741 cgctccggcc gagccggagc cattcgctgt agagcggcca gacgttcgcc tcgcagccct
     1801 cgagcgactt cgactgctgc tgcatgaccg ctgcccggtt gcccttcccg cagtagctca
     1861 cgtggccgag cccgagccag tggcccacct cgtggttcac gacgtagcgg cgatacgcgt
     1921 tgtagccgag cgtccacgcc tccgtcccgt ggcgccagcg gtagtcgttg atgagcacgt
     1981 cgtccccgac gcggcagctc cagggccggt cgcagacggg cgaggcggcg ttcacctcgg
     2041 ccggggaggc gaggatgagc cggaagtcgc cgccggacgg cacctcgacg aaccggctgt
     2101 gcccgccgag cgtccacccg cgttcgttcg tgagggcggt gcgcgcgatg tgccggaacc
     2161 actcgaggtc ggcctcgacg tcgccgcgcg acgcgatcga gtacgtgtag cggtgcgtgg
     2221 cggcggggcc cgcgagcgcc gccgccgtca ccgcgacgag gatagcggcg gccgtccgga
     2281 gcgaccgccg gcccctcatc cctccgccgc gaccgcctcg gcgacggtgt cgtggacggg
     2341 caggtacgtc gagagcccgg agacctggag cacccgctcg accgcctcgc cggccgaggc
     2401 gagcacgagc cgcccgccgt gcgcctccat ccgccgggcg gccagcagga ggaggccgag
     2461 caccgtcgag tcgaggaacg tcacgtcggc gaggtcgagc acgacgacgt gccggccgcc
     2521 gggcaccgcc tcctcgagct cccggcggag gacctcggcc gagaagaggt cgaggtcccc
     2581 cgccagcgtc acgacgtcga cgccgtcgtg ccgcgccgtc gacgtgagcg gctcaccggc
     2641 gatcgtcacg agagctccgg ctccgcgagc cgcgtctcga ggcggcgcgc gggatccttg
     2701 accgcccact ccgtcaggat cccgaccgcc tccgcggcga cccgctcctc gaccagcacg
     2761 tcgtagtacg tggcgtcgag ggtctgcacc gactcgaacg ggcgccgctc cgaccaggcg
     2821 tgcagcgcga gcgcgagcaa cgcgccgatc gcggcgccga ggatcgcgcc ccaggcgagc
     2881 gtcccggcga cgccgacatc ctcggacaga aggccgagaa ggaggccgac gccggcgccg
     2941 accacgacgc ccgtcccggc gccgtcggcc gcggcacgcc tgcgcgtccg cgtgccgacg
     3001 acgcgttcga caagcttcaa gtcgcgggcg acgatcgtcg cccgctcgat cggaaaccgc
     3061 gcggaggaga gccactcgat cgcatcggcg gcctcgtcgt atgtgggcac cgtggcgacg
     3121 cggaccggct cgagccgctc ctcgctcgcc gcgctgccgt tcatgcctgt tctatgtctg
     3181 gtcttcgttt ccatgccggg tcccttcccg ccgggccgcc ggtccagcgc ctgtcgcgct
     3241 cacgattcgt gcagcgtttc ctcagcggac gctgagggtt acggagccgc gagcttgtag
     3301 ccggccccac gcaccgtgag gatccgccgt gggttggccg ggtcagcctc gaccttccgg
     3361 cggaggttcg acacgtgcac ctcgcaggcg tgctcgtcgc cgacgaagtc cgtctgccag
     3421 aggtgctgca tgacctctcg gcgcgagaca acccggccgg gccgcgacgc gaggagcgtg
     3481 aggatcttga gctcggacgg cgtcacggcg atcggctcgc cgtccaccgt cacctcgtgc
     3541 cgcgcagggt cgacgcgcag cccgccgacc tcgagcgggg atgcggccgc cgccgagcgg
     3601 tccagctccc gccgccgaag gagcgcgcgc aggcggctca cgagctcggc cgaggagaac
     3661 ggcttcgtca cgtagtcgtc ggcgccgagc tcgagcccta gaacgcggtc gacctccgca
     3721 tccttcgccg tcagcatcac gatcggcacg ctcgactcgg cgcgaagggt gcggcagacg
     3781 tcgaagcccg agccgtccgg cagcatgacg tcgagcacga cgatgtcgta ctcctgctcg
     3841 cgcgcgtgcg cgagcgcggc ggaggcgtcc tcgaccggcg tgaccgcgaa cccttcccgc
     3901 tcgagggcgt aaccgaccac ctcgcggacg cccgcatcgt cttcggcgag gaggatccgc
     3961 gcgctcacac cgcctccagc cgcgccgcgc gaaggaggac ccgcgcaagt gtcccgccgc
     4021 cgggcgcggc atcgatgtgg acgtcgccgc cgagcgcgcg tgccgactcg cgggcgatcg
     4081 cgagcccgag gccgaagcgg tcggccgcgc gaccctcgac gcggtagaag cggtcgaaga
     4141 gccgctcgcg cgcccgggct ggcacacccg ggccggtgtc cgcgacctcg atcgcgacgc
     4201 cgtcgccgtt cgcctccgcc gagagccgga tcgtcccctc ctccgtgtgg tgcgccgcgt
     4261 tcgccgcgag gttcgcaacc gcctgctcga ggaggtccgc gtcggcgagc gcctcgaggc
     4321 ccggcgggca cgcgacctcg accctgacgc cgggcggcgc ctggagcgcg cccgcgacgg
     4381 cctcgagcag cggcaggatg cggacgggcg cgagctcgac gcgctcggcc ccggtttgcg
     4441 cgcgcgcgag cacgaggagc gcccgcgtca gccggctcag ccggcggccc tcgcgctcga
     4501 tgagggcgag gaagcgaccg cgcgcgtcgg ggtcgtcgag cgcgcccgcc tgcagcgcct
     4561 cgaccgcgcc gaggatcgtc tggagcggag tccggagctc gtgcgaggcg ttcgagacga
     4621 actcgcgctc ggcgcgccgg cggcgctcct gctcggagac gtcctcgagc acgagcatcg
     4681 ccgtgtccgc gccgtgcgcg gggatgccga cgacggcgag cgtccggtcg ccgtccacga
     4741 cgcgttcgtg cgccacggtg gcgccggggc ggaagagctc ggcggcgagc gcgccgaggc
     4801 ggggctccgg caggggcgcg ccctcttcga ggcgcgcggg cgcgagaagc tccgcggcgg
     4861 cctcgttcgc gaactcgacc gtgccgtccg gggcaatcgt gacgacgccc tcgtggaggc
     4921 gttcgagcag ccgttcgagg cggtcgcgct cccggccgag ctcggtgaac gcctcgcgga
     4981 ggtggagccg catccgatcg agcgacgtgg cgagcgcgcc gacctcgtcg ggaaagcgag
     5041 cggtcaccgg ccggtcgaag tcgcgcgcct cgatcgccgc cgccgatgcg gcgatgcggc
     5101 gcagccgccg cgcgatgagc gcggcgacga ggattccggc aaggccgccg accagcacgc
     5161 cccagagcgc cgcctcggtg atggtcgagc gggcaaggcc caacccggcc tcgagctccg
     5221 ggtggctcgc gaacgcgacg accgcgctcg ccggcccgct ccgcaccggg agggcgacga
     5281 ccgttcccgc gccgtcgccg gcggtctcta cgtaccgccg cccggcgagc gcctcgtcga
     5341 gcgccgttcc
//
LOCUS       DASTFC010000138         5764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_612236,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000138 DASTFC010000000
VERSION     DASTFC010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612236"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFO88_04115"
     CDS             complement(<1..275)
                     /locus_tag="VFO88_04115"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET9544740.1"
                     /translation="MPQAVVLSAVRTPVGRYGGALAGVRPDDLGAIAVRAAVERSGVP
                     AGEIEDVYLGCANQAGEDNRNVARMAALLAGLPQEVAGVTVNRLCASG"
     gene            343..1041
                     /locus_tag="VFO88_04120"
     CDS             343..1041
                     /locus_tag="VFO88_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544741.1"
                     /translation="MRPAALALLLTLVLALAACGDDEGNGGATTGAETAETTPAFEDP
                     EPLPRTEVVGGYLYEVEEDGFEIAVPRRWVARSAAQVPDPRAIRRLSVDYPVIIGYFE
                     ALVGDSPTRFVAAAPNLRKNFATNLTVTIEEVEAGTTTEQYEEETYAEILSTAANVRG
                     EIRRSDAQLPVGPVRVLRYERSFSDEIALETRHYFFVDGERAFALTYSTALPDPAFDA
                     VVERSTESFRLTGD"
     gene            complement(1082..1912)
                     /locus_tag="VFO88_04125"
     CDS             complement(1082..1912)
                     /locus_tag="VFO88_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET9544742.1"
                     /translation="MLIRLGHSPDPDDAFMFWGLAEGPVDPGEFEFEHVLKDIQTLNE
                     WALEGRLEVSAISLHAYPSVQDTYVILPHGASMGSGYGPIVVATEELSLDDLRGLEIA
                     VPGRMTTAFLALRLCLGGEFAYREVPFDEIIAEVKSGAARAGLLIHEGQLTYAQQGLR
                     KIVDLGEWWLLETGLPLPLGINVARRDLGDDALLRLSDVLAASIEAGLDHRPQALRYA
                     MRFGRGLDPALTDRFVGMYVNRLTQDYGDEGRKAVAELLSRAEQAGALERPVRVEFVG
                     "
     gene            1982..2641
                     /locus_tag="VFO88_04130"
     CDS             1982..2641
                     /locus_tag="VFO88_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET9544743.1"
                     /translation="MASNDLSEAIQDYVKEIYKLGAAGERATTSAIARRMGVAPSSAT
                     SMLKKLAALGLAEHAPYRGAVLTDAGERIALEVIRHHRLLEQYLAETLGLGIDAVHAE
                     ADRLEHVLSDELERRIDEALGFPTHDPHGHPIPDADLQVAPSTLRSLTALEPGEEATV
                     RRVPDGDADVLRYLADLDLVPGGRVRMGRCEPFEGPVLVTVGEREHAISRELAGQIGV
                     A"
     gene            complement(2642..2989)
                     /locus_tag="VFO88_04135"
     CDS             complement(2642..2989)
                     /locus_tag="VFO88_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544744.1"
                     /translation="MAQDSGSMRFAERTLHGLGENGDRDTISIWIERRPGAVWAVGRA
                     IDIDARRTPEPRPDDYIFEGYELGDALEAANNALSDDLQISRGDGVNEDVALFVEEEL
                     LKPLERWFFGHKP"
     gene            3015..3287
                     /locus_tag="VFO88_04140"
     CDS             3015..3287
                     /locus_tag="VFO88_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544745.1"
                     /translation="MGFFSRLFGKKDEGESAEEQRGSISKEEALAAYIVREHKQGRSL
                     EEILDDPYLKNRATEEQRIRLLERPEVIRAIGEDTAEMARDRVRES"
     gene            complement(3284..4213)
                     /locus_tag="VFO88_04145"
     CDS             complement(3284..4213)
                     /locus_tag="VFO88_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET9544746.1"
                     /translation="MPLFLREAEVEELLPPADAVEAVEGCFERLARGVVENRPRFRLR
                     LDAGRLHVMAAADLELGVAGHKSYTGFAEGARFLLALWAADRPELLALIEADRLGQRR
                     TGAASAVAARRLAKPNARTLGVIGCGWQAEAQVECIRAALPGIEEVVAYCRDDERRRA
                     FCERHRAEEAEWYRDAAEADVVVTVTTSRDPVLRGEWLKPGALVCAVGANRIEARELD
                     NVVLERAAFVCCDSREQAALEAGDLVEPVERGVLDWLEVHELAEVFLGELPGRQSDDD
                     IVLFKSLGIAAEDLAAGKLVLDRARERGTGIEL"
     gene            4234..4533
                     /locus_tag="VFO88_04150"
     CDS             4234..4533
                     /locus_tag="VFO88_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9544747.1"
                     /translation="MSAPVRVPLEALGPRGRTVVETEAGPVAVFLVDGRAHAVANRCP
                     HEGNPLVEGEVAGGVLTCVFHLWRFDLESGACLFGDAPVARYEAAVEGGEIVVRL"
     gene            complement(4530..5444)
                     /locus_tag="VFO88_04155"
     CDS             complement(4530..5444)
                     /locus_tag="VFO88_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9544748.1"
                     /translation="MRICSLVPAATEVLFALGLGDSVVGVSHECDFPPEATSRPVVTA
                     SLVETGELSSAAVDDAVRAASSNGHPLYAVDAERWAEVAPDVVVTQELCAVCAVSSDD
                     VGAVVEATGLDTETIAYSPGTLDGIVDGIEELGRRFGADGEADELVAGMRGRLERVGA
                     ALASVETAPRVFVAEWLEPPYTAGHWVPDMVAAAGGMDVAGLAGERSYRLQWSDVARL
                     APDVVVLAPCGFDLDRTLAEIAPLELAGHLLGTPAREESRVFAVDADAMFSRPGPRVV
                     DGVELLAYLLHPQAYGDPGFPWSRVRVA"
     gene            complement(5657..5729)
                     /locus_tag="VFO88_04160"
     tRNA            complement(5657..5729)
                     /locus_tag="VFO88_04160"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5694..5696),aa:Arg,seq:gcg)
BASE COUNT          793 a         2134 c         2119 g          718 t
ORIGIN      
        1 cccgacgcgc agagccggtt cacggtgacg cccgcgacct cctggggcag gcccgcgagg
       61 agcgccgcca tgcgggcgac gttgcggttg tcctcgcccg cctggttcgc gcagccgagg
      121 tagacgtcct cgatctcgcc ggcggggacg ccggagcgct ccaccgcggc gcggacggcg
      181 atcgcgccga ggtcgtcggg acgcacgccg gcgagcgcgc ccccgtaacg gccgacgggc
      241 gtccggacgg cggagaggac cacggcctgg ggcacgagcg cgagtctagc ccccgccggt
      301 tggcccgccg acccgagcgc gacgcgtgcc acacttggcc gcgtgcgccc cgccgccctg
      361 gccctcctcc tgacgctggt cctcgcgctc gccgcctgcg gcgacgacga gggaaacggc
      421 ggcgcgacga ccggcgcgga gacggccgag acgacgcccg ccttcgagga tcccgagccc
      481 cttccgcgca ccgaggtggt cggcggctac ctctacgagg tcgaggagga cggcttcgag
      541 atcgcggtgc cgcggcgctg ggtcgcccgg agcgcggcgc aggtgcccga cccgcgcgcg
      601 atccgccggc tctcggtcga ctacccggtg atcatcggct acttcgaggc gctcgtcggc
      661 gactcaccca cccgcttcgt ggccgcggcg ccgaacctcc gcaagaactt cgccacgaac
      721 ctcaccgtca cgatcgagga ggtcgaggcc gggacgacga cggagcagta cgaggaggag
      781 acgtacgccg agatcctttc gacggcggcg aacgtgcggg gcgagatccg gcggagcgac
      841 gcgcagctcc ccgtcgggcc cgtgcgcgtg ctccgctacg agcgcagctt ctcggacgag
      901 atcgccctcg agacccggca ctacttcttc gtcgacggcg agcgcgcctt cgcgctcacg
      961 tactcgaccg cgctccccga tcccgcgttc gacgccgtcg tcgagcgctc gacggagtcg
     1021 ttccggctca cgggggacta gctccgcctc cgcgcgctcg ttcaggctgg tctgggagcg
     1081 ctcagccgac gaactcgacg cggacgggcc gctcgagggc gcccgcctgc tccgcgcggg
     1141 agaggagctc ggcgaccgcc ttgcggccct cgtcgccgta gtcctgcgtc agccggttca
     1201 cgtacatccc gacgaagcgg tcggtcagcg ccgggtcgag cccgcgcccg aaccgcatcg
     1261 cgtagcggag cgcctgcggc cggtggtcga gcccggcctc gatcgacgcg gcgaggacgt
     1321 ccgagagccg gagcagcgcg tcgtcgccga ggtcgcggcg cgcgacgttg atcccgaggg
     1381 ggagcggcag cccggtctcg agcagccacc actcgccgag gtcgacgatc ttccggagcc
     1441 cctgctgggc gtacgtgagc tggccctcgt ggatgaggag cccggcgcgc gcggcgcccg
     1501 acttgacctc ggcgatgatc tcgtcgaacg gcacctcccg gtaggcgaac tccccgccga
     1561 ggcagaggcg cagcgcgagg aaggccgtgg tcatccggcc ggggacggcg atctcgagcc
     1621 cgcgcaggtc gtcgagggag agctcctcgg tcgcgacgac gatcggcccg tagcccgagc
     1681 ccatcgaggc gccgtgcggg aggatcacgt aggtgtcctg caccgacggg tacgcgtgga
     1741 gcgagatcgc gctcacctcg aggcgcccct cgagcgccca ctcgttgagc gtctggatgt
     1801 ccttcaggac gtgctcgaac tcgaactcgc cggggtcgac gggaccctcg gcgaggcccc
     1861 agaacatgaa ggcgtcgtcg ggatccgggc tgtggccgag gcggatcagc aagcgcccga
     1921 gggtagcacc ctccctccgc cgcggtgggg ggaagcgtcg gcgcggatta cgatttgccg
     1981 cgtggcgtcg aacgacctga gcgaggcgat ccaggactac gtgaaggaga tctacaagct
     2041 cggggcggcc ggcgagcggg cgacgacgtc cgcgatcgcg cgccggatgg gggtcgcgcc
     2101 gtcgtccgcg acctcgatgc tgaagaagct cgccgcgctc ggcctcgccg agcacgcccc
     2161 gtaccgcggc gccgtgctga cggacgcggg cgagcggatc gcgctcgagg tgatccggca
     2221 ccaccgcctg ctcgagcagt atctcgccga gacgctcggg ctcgggatcg acgcggtgca
     2281 cgcggaggcc gaccgcctcg agcacgtgct ctcggacgag ctcgagcggc ggatcgacga
     2341 ggcgctcggc ttcccgaccc acgacccgca cgggcacccg attcccgacg ccgacctcca
     2401 ggtggcgccc tcgacgctgc gctcgctcac cgccctcgag ccgggcgagg aggcgaccgt
     2461 gcggcgcgtg cccgacggcg acgcggacgt ccttcgctac ctcgcggacc tcgacctggt
     2521 ccccggcggc cgcgtgcgga tggggcgctg cgagccgttc gaggggcccg tgctcgtcac
     2581 ggtcggcgag cgcgagcacg cgatctcgcg cgagctcgcg ggccagatcg gcgtcgccta
     2641 gctacggctt gtggccgaag aaccagcgct cgaggggctt cagcagctcc tcctccacga
     2701 agagcgcgac gtcctcgttc acgccgtcgc cgcgcgagat ctggaggtcg tccgagagcg
     2761 cgttgttcgc ggcctcgagc gcgtcgccga gctcgtagcc ctcgaagatg tagtcgtccg
     2821 gccgcggctc gggcgtccgg cgcgcgtcga tgtcgatcgc ccgtccgacc gcccagacgg
     2881 ctcccggccg ccgttcgatc cagatggaga tcgtgtcgcg gtcaccgttc tcgccgagcc
     2941 cgtgcagcgt ccgctcggcg aagcgcatcg accctgagtc ctgggccacg agagcacgat
     3001 acactcatgc cgcgatgggc ttcttcagtc gactgttcgg caagaaggac gagggcgaga
     3061 gcgccgagga gcagcgcggg agcatctcga aggaggaggc gctcgccgcg tacatcgtgc
     3121 gggagcacaa gcagggccgc tcgctcgagg agatcctcga cgacccgtac ctgaagaacc
     3181 gcgcgacgga ggagcagcgc atccggctgc tcgagcgccc cgaggtcatc cgcgcgatcg
     3241 gcgaggacac cgccgagatg gcccgcgacc gcgtccgcga gagctagagc tcgatcccgg
     3301 tcccgcgctc gcgcgcccgg tcgagcacga gcttgccggc ggcgaggtcc tcggcggcga
     3361 tgccgaggct cttgaagagg acgatgtcgt cgtccgactg gcggccgggc agctcgccga
     3421 ggaagacctc cgcgagctcg tgcacctcga gccagtcgag aacgccgcgc tcgaccggct
     3481 cgacgaggtc gcccgcctcg agcgcggcct gctcgcgcga atcgcagcag acgaacgccg
     3541 cccgctcgag gacgacgttg tcgagctccc gcgcctcgat ccggttcgcg ccgacggcgc
     3601 agacgagcgc gcccggcttg agccactcgc cgcgcagaac ggggtcgcgc gaggtcgtca
     3661 ccgtgacgac gacgtccgcc tcggccgcgt cgcggtacca ctcggcctcc tcggcgcggt
     3721 ggcgctcgca gaacgcccta cggcgctcgt cgtcgcggca gtaggcgacg acctcctcga
     3781 tcccgggaag cgcggcgcgg atgcactcga cctgcgcctc ggcctgccag ccgcagccga
     3841 tcaccccgag cgtgcgcgcg ttcggcttgg ccagccgccg cgccgcgacc gcgctcgccg
     3901 cgcccgtgcg tcgctggccg agccggtcgg cctcgatgag cgcgagaagc tccggccggt
     3961 cggccgccca gagcgcgagc aggaagcggg cgccctcggc gaagccggtg tagctcttgt
     4021 gcccggcgac gccgagctcg aggtcggcgg ccgccatcac gtgcagtcgg cccgcgtcga
     4081 gcctcagccg gaagcgcggc cggttctcga cgacgccgcg ggcgagccgc tcgaagcagc
     4141 cctcgaccgc ctcgacggcg tcggccggag gcaggagctc ctcgacctcg gcctcgcgca
     4201 ggaagagcgg catggcggag tatcgtaggc ggcgtgtcgg cgccggtccg cgtcccgctc
     4261 gaggcgctcg gcccgcgcgg gcggacggtc gtcgagaccg aggccggccc cgtcgccgtc
     4321 ttcctcgtcg acggccgggc gcacgcggtc gcgaaccgct gtccgcacga ggggaacccg
     4381 ctcgtcgagg gcgaggtggc gggcggcgtc ctcacgtgcg tcttccacct ctggcgcttc
     4441 gacctcgagt cgggcgcctg cctcttcggc gacgcgccgg tcgcccgcta cgaggcagcc
     4501 gtcgagggcg gcgagatcgt cgtgcggctc taggccacgc gcacccggct ccagggaaag
     4561 ccggggtcgc cgtacgcctg cgggtggagg aggtaggcga gcagctcgac gccgtcgacg
     4621 acgcgggggc cggggcggga gaacatcgcg tccgcgtcga ccgcgaagac gcggctctcc
     4681 tcgcgcgccg gcgtgccgag caggtgcccg gcgagctcga gcggcgcgat ctcggcgagc
     4741 gtccggtcga ggtcgaaccc gcacggcgcg agaacgacga cgtcgggagc gaggcgcgcg
     4801 acgtcgctcc actggagccg gtaggaccgc tcgcccgcga gccccgcgac gtccatgccg
     4861 ccggccgccg cgaccatgtc cgggacccag tgccccgcgg tgtagggcgg ctcgagccac
     4921 tcggcgacga agacccgcgg cgcggtctcg acgcttgcga gcgccgcgcc gacgcgctcg
     4981 agccggccgc gcatcccggc gacgagctcg tccgcctcgc cgtcggcgcc gaaccgccgc
     5041 ccgagctcct cgatcccgtc gacgatcccg tcgagcgtgc ccggcgagta cgcgatcgtc
     5101 tcggtgtcga gcccggtcgc ctccacgacc gcgccgacgt cgtcggagga gacggcgcag
     5161 acggcacaga gctcctgcgt gacgacgacg tcgggcgcga cctcggccca gcgctcggcg
     5221 tcgaccgcgt agagcgggtg gccgttcgag gacgcggcac gcacggcgtc gtcgaccgcc
     5281 gcgctcgaga gctcgccggt ctcgacgagc gacgcggtca cgaccggccg cgaggtcgcc
     5341 tccggcggga agtcgcactc gtgcgacacc ccgacgaccg agtcgccgag gccgagcgcg
     5401 aacaagacct ccgtcgcggc gggaacgagc gagcagatcc gcaaggccgg ctcagcgtag
     5461 cccatgctcg aggcggtccc cgcgccgcgg cgggctccgc ccgtcccggc tgcatcccga
     5521 ggccgggaga gaccgacccg gatcgaaccc cggggtcagg tcttgtgcta tcgcatcggg
     5581 acccccgctc ccggtctcgt cgcgacctgg atcgtcacgg cagccgcgga gcggtttccg
     5641 gatccacggt cggtgatgcg cccggcacga ttcgaacgtg cgacctcagc ctccgcaggg
     5701 ctgcgctcta tcccctgagc tacgggcgcg cgagaggtca gtctagcggc cgggcggcgg
     5761 cgcc
//
LOCUS       DASTFC010000139         9323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1419954,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000139 DASTFC010000000
VERSION     DASTFC010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419954"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFO88_04165"
     CDS             complement(<1..705)
                     /locus_tag="VFO88_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9544749.1"
                     /translation="MRRTLAAASALSVLAAAGTAHAEPNWDANVARAEAYAATRAGVE
                     SFVVRDESGRGHGRGVRRVVHSASVLKAMLMVAYLRQPSVRHRDLTDADRDLLRPMIR
                     WSDNATASRLVVMLGPAPLYDLAARAQMGHFRLDSPWGASEITARGQSKFFRRIERLM
                     PRRHREYGMRLLNRIVPSQRWGIPPVKPEGWRIYFKGGWGDGSGVVTHQVALLKRGDR
                     RVSLAILTTANPSHPYG"
     gene            complement(705..1604)
                     /locus_tag="VFO88_04170"
     CDS             complement(705..1604)
                     /locus_tag="VFO88_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9544750.1"
                     /translation="MRLAGIHHVALRVFDLDEAAERWSRQFGFTVRERHDDRVLLACA
                     FEDYSLELLASGERPGFDHAGWELAPGETWGDRDGEVEERPGGRARSLHLADPDGYGV
                     ELVPWTERADPFPDVARLSTDLPPFHPRKLGHVNVLTQDVRRLSSFYVDELGFAVSDR
                     LGDEGIWLHLNADHHVHAMLEKDVTHFHHFALELRDIGEVRLLLDHLARHGRWVVWGP
                     GRHGVAASIFAYIRIPEEELIVELYCDMEQLLPGHEPRDWEDTPHSSNTWGTLPPRTY
                     FRFDAEAIELERGQLQALGRAPS"
     gene            complement(1612..2106)
                     /locus_tag="VFO88_04175"
     CDS             complement(1612..2106)
                     /locus_tag="VFO88_04175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET9544751.1"
                     /translation="MSVDPRQFRDAMGHFATGVTVLTTRHEGELHGMTANSVTSVSLD
                     PMLILVCLMKDARTALAIQAAERFAVNILTEGQDELSQRFARPGQDHFEGLELMEGPH
                     GLPLLPGCLAHLAAQVTEIVPAGDHDVVFGEVDHVHVNPNGGAPLLFFQGGYRKLPGM
                     TRLG"
     gene            complement(2285..3748)
                     /locus_tag="VFO88_04180"
     CDS             complement(2285..3748)
                     /locus_tag="VFO88_04180"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET9544752.1"
                     /translation="MATTQETTGARTGEQFLAGLRSEERDIWLEGERITDPSSHPKLE
                     GAARSLARLFDLQHEDPETFLMESPDTGQPVSVTHVLPKSREDLERRRIASKRIADAT
                     AGMMGRTPDYLNYTFACFAARSDVWARYGNEEGAANLVAYQKLMRDNDLSLTHTLINP
                     QVDRSVPEAEQAAGEVSLHKVADTENGILVRGARMLATLAPFADELAVYPGSDLRLQD
                     GKYAICFAIPMTTPGLRFICRDSFSVDRDAWDYPLSTRFDEMDAVVIFDDVEIPQERI
                     FLDGDPQLHTEVITDTHWRAHIIHQAMTRAWAKLEFAFGLGHTMADLTGVNRFDHVQE
                     KLGELWSMLELTRAGVIAAEAGSFRAEDADDWVPDERSFVALRGLMPKWIPRAIELLQ
                     LVGGGGFMATPSKADFANEALRPEIDKYFQARNADAERRTRAYRLAWDFVGSALAGRG
                     ELYERFYLSDSFRMTALAYVLADKEHPTELVEQFLAD"
     gene            complement(3754..5241)
                     /locus_tag="VFO88_04185"
     CDS             complement(3754..5241)
                     /locus_tag="VFO88_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HET9544753.1"
                     /translation="MFGRAARRRERLEVSATAVVAGVEVSPDHFIGGERVSSAATFPD
                     ISPIDESHLADIARGGGAEIDQAVAAAQDAFPAWAALGPEGRAQHLFAIADLILERQD
                     MLAEVETLDNGSLLEASRLRLMKRASANFRFFAQLALDLEGRRWDVDGTRYAVRRDPS
                     GVAALIAPWNAPFMLETWKCGPALAAGCTAVLKPAEWSPLTASLLGEIVTEAGLPPGV
                     LNVVQGIGEEAGAALVAHPGLARISFTGSVETAQSIARAAAANLTPVSFELGGKSPFI
                     VFADADLDRALETATYQYDNAGQVCLAGTRLLVEQTIADEFTERFLAGVAERRLGDPR
                     DPDTDIGPLITREHLARVEGFVERAEADGAELLFGGRVSPELGGLYYEPTLFTNVRQE
                     MEIVQKEVFGPVLTLQTFADEDEAVALANGTEYGLAATVFTTDDARAERLGERLVAGT
                     TWINCFYARDLGAPFGGAKRSGIGREGGEWSFDFYADVKTVARRD"
     gene            complement(5384..6106)
                     /locus_tag="VFO88_04190"
     CDS             complement(5384..6106)
                     /locus_tag="VFO88_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9544754.1"
                     /translation="MRHPVELAPSKIVAVHLSYPSRVEEYAARTPPDPSYFLKPPSSL
                     ARHGDEIVRPLGCRYLNYEGEVAVVVGKRMKRVPVEDALDHVAAYSAANDFGVHDFRH
                     ADRGSMLRVKGQDGFCPIGPELVDAADVNPENLTLRTYVNGEVVQEGNTGELLWSIAY
                     MLADLSRLVTLEPGDVFLSGTPANSRPVAPGDTVEVEVEGIGRLSNRIVEAREPLEPI
                     GVMPEDTPNARHVALAVPEETA"
     gene            complement(6103..6900)
                     /locus_tag="VFO88_04195"
     CDS             complement(6103..6900)
                     /locus_tag="VFO88_04195"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HET9544755.1"
                     /translation="MDVTTPHGLTGYSLPQTRTGRSSLVPPPPWHYSGDFLIVEFRTD
                     PDAARALLPPELEPGDDLGSAAAIFVDWQSCSESGDELVDPVRAQYKEFFVVLAAEYR
                     GEAVTRCPFMWVDQGMPLVRGLVQGFPKKPGSIWITRPVTVGKAGPRLEPGGRFGASL
                     ASYDRRLAEATLTLTGPSEEGPTVNSPPLWNTRLFPAWETDGDPLQEHVWAGGEDREI
                     SPIWEGDATLRFFESPADELADLRPTEVGKGFWFSFGYTVTGGRRVE"
     gene            complement(6933..8375)
                     /locus_tag="VFO88_04200"
     CDS             complement(6933..8375)
                     /locus_tag="VFO88_04200"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HET9544756.1"
                     /translation="MRVRLAFVSVLALAAFAAAGAAQAATEPGKASARGLAIRVVLPD
                     GQSEALAEAVAPPARQATSGSLVYGDGIVTTGSVWARARASTRPKSGEAWASSTLRTV
                     SIFGGEITIGAVSTKASADARPAGASGSLAGSWLARVQILGETVRPSRNARVQLGDWG
                     YAILLEQAVVRDPKRVGRRTFVTGLHVYLTADHGGLPAGAEILVGYAEAAASAPRKPA
                     DEAEPEQPTGSPLAPPKGPKRDPEPPKPGLPKVPPPVIQNPPASVRPAVTSEGFVFPV
                     YGPSSFVDDFAASRATTGWHHGNDIFAPVGAPVLAVTEGTLFLVGWNDVGGLRAWLRD
                     RQGNEYYYAHLSAFSPLAFQGSQVRAGDVIGFVGATGDAVGTPPHLHFEIHPVGLLGL
                     GYDGVINPYEYLLAWQRVADATFDWGVAQPGQAPAPGIVLLQAEDISAASGLDPEALT
                     RFLELPAFFGEPVARVEPTVVGADPGFDGG"
     gene            complement(8444..8821)
                     /locus_tag="VFO88_04205"
     CDS             complement(8444..8821)
                     /locus_tag="VFO88_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9544757.1"
                     /translation="MTTVILALALGFAAGVLSGTFGVGGGILFVPTLTLVVGLSQLGA
                     QATSLAAMIPVVVLGAWRQHRVGAVDWRAATIAGLASGVGVAGGALLADALGDETLRR
                     LFAGLLVVTAAHLAWSASRSPRP"
     gene            complement(8818..9183)
                     /locus_tag="VFO88_04210"
     CDS             complement(8818..9183)
                     /locus_tag="VFO88_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9544758.1"
                     /translation="MPRLTSSAPTLVGIGLTGGFFGALFGVGGGIVVIPLLIFAGFRV
                     KVATATSLAIIGFTAVFGVIAFGALGHVSWGDAALVGLPALGGTLAGTWAHRRLSSRM
                     LTFLFAGFLVLVAIRLVLE"
BASE COUNT         1317 a         3294 c         3501 g         1211 t
ORIGIN      
        1 cccgtacgga tggctcgggt tcgccgtcgt gaggatcgcg agcgacacgc gccggtcgcc
       61 gcgcttcagg agcgcgacct ggtgcgtgac gacgccgctc ccgtcgcccc agccgccctt
      121 gaagtagatc cgccagccct ccggcttcac gggcgggatc ccccagcgct gcgaggggac
      181 gatccggttc agcagccgca tcccgtactc gcggtggcgg cggggcatca gtcgctcgat
      241 ccggcggaag aacttcgact gcccgcgcgc ggtgatctcc gacgcccccc acggcgagtc
      301 gaggcggaag tgacccatct gcgcgcgggc ggcgaggtcg tagagcggtg cggggccgag
      361 catcacgacg agccgcgagg cggtcgcgtt gtccgaccag cggatcatcg ggcgcaggag
      421 gtcgcggtcc gcgtccgtga ggtcgcggtg ccggacggac ggctgccgca ggtacgcgac
      481 catgagcatc gccttgagga cgctcgccga gtggacgacc cggcggacgc cgcgcccgtg
      541 cccgcggccg ctctcgtcgc ggacgacgaa ggactcgacg cctgcacgcg tcgccgcgta
      601 ggcctcggcc cgcgcgacgt tcgcgtccca gttcggctcg gcgtgcgccg ttccggcggc
      661 cgccagcacg gagagggccg acgccgccgc gagcgtgcgg cgcatcagct cggcgcgcgt
      721 ccgagggcct ggagctggcc gcgctcgagc tcgatcgcct cggcgtcgaa gcggaagtac
      781 gtccgcggcg ggagcgtccc ccacgtgttc gagctgtgcg gcgtgtcctc ccagtcgcgc
      841 ggctcgtggc cggggaggag ctgctccatg tcgcagtaga gctcgacgat cagctcctcc
      901 tccggaatcc tgatgtacgc gaagatcgac gcggcgacgc cgtggcggcc ggggccccag
      961 acgacccagc gcccgtgccg ggcgaggtgg tcgaggagga gccggacctc gccgatgtcg
     1021 cgcagctcga gcgcgaagtg gtggaagtgc gtgacgtcct tctcgagcat cgcgtggacg
     1081 tggtggtcgg cgttcaggtg gagccagatc ccctcgtcgc cgagccggtc ggagaccgcg
     1141 aagccgagct cgtccacgta gaaggaggac aggcgccgga cgtcctgcgt gaggacgttc
     1201 acgtggccga gcttgcgcgg gtggaacggc ggcaggtcgg tcgagagccg cgccacgtcg
     1261 gggaacgggt cggcgcgctc cgtccacggg acgagctcga cgccgtaccc gtccggatcc
     1321 gcgaggtgga gcgaccgggc gcgcccgccg gggcgctcct cgacctcccc gtcgcggtcg
     1381 ccccacgtct cgccgggagc gagctcccac cccgcgtggt cgaagcccgg ccgttcgccg
     1441 gacgcgagga gctcgagcga gtagtcctcg aacgcgcacg cgagcaggac gcggtcgtcg
     1501 tggcgctccc gcacggtgaa gccgaactgc cgcgaccacc gctccgccgc ctcgtcgagg
     1561 tcgaagacgc gcaacgcgac atggtggatg ccggcgagcc tcacgccgca atcatcccag
     1621 acgcgtcatc ccggggagct tccggtagcc gccctggaag aacaggagcg gcgcgccgcc
     1681 gttcgggttc acgtgcacgt ggtcgacctc gccgaacacg acgtcgtgat cgcctgccgg
     1741 gacgatctcc gtcacctgcg ccgccagatg ggcgaggcag cccggcagga gcggcagccc
     1801 gtgcgggccc tccatcagct cgaggccctc gaaatgatcc tggcccggac gggcgaagcg
     1861 ctgcgagagc tcgtcctgcc cctcggtgag gatgttgacc gcgaaccgct ccgccgcctg
     1921 gatcgcgagc gccgtgcgcg cgtccttcat caggcagacg aggatcagca tcgggtcgag
     1981 cgagacgctc gtgaccgagt tcgccgtcat cccgtgcagc tcgccctcgt gccgcgtcgt
     2041 cagcaccgtg acgccggtgg cgaagtgccc catggcgtcg cggaactggc gcggatcgac
     2101 ggacacgcga tgatcgccgg ctagggcgac tcctggtatc ccgcccccgg ctccgggtcg
     2161 ttccgaccaa gccaggcaag gacgcaggga gcgtcgatgc tagaagcatc ggggcgactg
     2221 aggacgccgc gtggcgcagc gtcggggcgg gccggagacg atcggtgggg tgcctggagg
     2281 tgcactagtc ggcgaggaac tgctccacga gctcggtcgg gtgctccttg tcggcgagga
     2341 cgtaggcgag cgcggtcatg cggaacgagt cggagaggta gaagcgctcg tagagctcgc
     2401 cgcggcccgc gagcgcggag ccgacgaagt cccaggcgag ccggtaggcg cgcgtgcgcc
     2461 gctcggcgtc cgcgttccgg gcctggaagt acttgtcgat ctccggccgc agcgcctcgt
     2521 tcgcgaagtc ggccttcgac ggcgtcgcca tgaacccgcc gccgccgacg agctggagca
     2581 gctcgatcgc gcgcgggatc cacttcggca tgagcccgcg gagcgcgacg aacgagcgct
     2641 cgtccggcac ccagtcgtcc gcgtcctcgg cccggaacga gccggcctcc gcggcgatca
     2701 cgccggcgcg cgtcagctcg agcatcgacc agagctcgcc gagcttctcc tgcacgtggt
     2761 cgaagcggtt cacgccggtc aggtcggcca tcgtgtgccc gagcccgaac gcgaactcga
     2821 gcttcgccca ggcgcgcgtc atcgcctggt ggatgatgtg cgcgcgccag tgcgtgtcgg
     2881 tgatcacctc ggtgtggagc tgggggtcgc cgtcgaggaa gatccgctcc tgcgggatct
     2941 cgacgtcgtc gaagatgacg accgcgtcca tctcgtcgaa gcgcgtcgag agggggtagt
     3001 cccaggcgtc gcggtcgacg ctgaacgagt cgcggcagat gaagcggaga ccgggcgtcg
     3061 tcatcgggat cgcgaagcag atcgcgtact tcccgtcctg gaggcgcagg tcggagccgg
     3121 ggtagacggc gagctcgtcg gcgaacggcg cgagcgtcgc gagcatgcgc gcgccgcgga
     3181 cgaggatgcc gttctccgtg tccgccacct tgtggagcga gacctcgccg gccgcctgct
     3241 cggcctcggg cacggagcgg tcgacctgcg ggttgatgag cgtgtgcgtg agcgacaggt
     3301 cgttgtcgcg catgagcttc tggtaggcga cgaggttcgc cgcgccctcc tcgttgccgt
     3361 agcgcgccca gacgtcggac cgggcggcga agcacgcgaa cgtgtagttc aggtagtcgg
     3421 gcgtgcggcc catcatcccg gcggtcgcgt cggcgatccg cttgctcgcg atgcggcgcc
     3481 gctcgaggtc ctcccggctc ttcggcagga cgtgcgtgac gctgaccggc tggccggtgt
     3541 cgggcgactc catgaggaac gtctccgggt cctcgtgctg gaggtcgaag aggcgcgcga
     3601 ggctgcgtgc ggcgccctcg agcttcgggt ggctcgacgg gtcggtgatg cgctcgccct
     3661 cgagccagat gtcgcgctcc tcgctccgga gccccgcgag gaactgctcg cccgtgcgtg
     3721 cgcctgtcgt ctcctgcgtg gttgccatct gcgtcagtcc ctcctggcca cggttttcac
     3781 gtctgcgtag aagtcgaagc tccactcgcc gccctcgcgc ccgatcccgg agcgcttggc
     3841 gcccccgaac ggcgcgccga ggtcgcgcgc gtagaagcag ttgatccagg tcgtgccggc
     3901 gacgagccgc tcgccgagcc gctcggcgcg cgcgtcgtcc gtcgtgaaca cggtcgccgc
     3961 gaggccgtac tcggtgccgt tcgcgagcgc gaccgcctcg tcctcgtccg cgaacgtctg
     4021 gagcgtgagg acggggccga agacctcctt ctggacgatc tccatctcct gccggacgtt
     4081 cgtgaagagc gtcggctcgt agtagagccc gccgagctcg ggcgagacgc ggccgccgaa
     4141 gaggagctcg gcgccgtcgg cctccgcgcg ctcgacgaag ccctcgaccc gcgcgaggtg
     4201 ctcgcgggtg atgagcgggc cgatgtccgt gtcggggtcg cgcgggtcgc cgagcctgcg
     4261 ctccgcgacg cccgcgagga agcgctcggt gaactcgtcg gcgatcgtct gctccacgag
     4321 gagccgcgtg cccgcgaggc agacctggcc cgcgttgtcg tactggtacg tagccgtctc
     4381 gagcgcgcgg tcgaggtcgg cgtcggcgaa gacgatgaac gggctcttcc cgccgagctc
     4441 gaacgagacc ggcgtcaggt tcgcggccgc cgcgcgggcg atcgactggg ccgtctcgac
     4501 cgagccggtg aaggagatcc gcgccagccc ggggtgggcg acgagcgcgg cacccgcctc
     4561 ctcgccgatc ccctggacga cgttcaggac gccgggcggg aggcccgcct cggtgacgat
     4621 ctcgccgaga agcgaggcgg tgagcggcga ccactcggcc ggcttgagga cggcggtaca
     4681 gccggccgcg agcgcggggc cgcacttcca cgtctcgagc atgaacggcg cgttccacgg
     4741 cgcaatgagc gcggcgacgc cggacgggtc gcgccggacg gcgtagcgcg tgccgtccac
     4801 gtcccagcgg cgtccctcga ggtcgagcgc gagctgcgcg aagaagcgga agttcgccga
     4861 ggcgcgcttc atgagccgca ggcgcgaggc ctcgaggagc gagccgttgt cgagcgtctc
     4921 gacctcggcg agcatgtcct gccgctcgag gatcaggtcg gcgatcgcga acaggtgctg
     4981 cgcgcgcccc tccgggccga gcgcggccca cgcggggaac gcgtcctgcg cggcggcgac
     5041 cgcctggtcg atctcggccc cgccgccgcg ggcgatgtcg gccaggtggc tctcgtcgat
     5101 cgggctgatg tcggggaacg tcgccgccga cgagacgcgc tcgccgccga tgaagtggtc
     5161 cggcgagacc tcgacgcccg cgacgaccgc cgtcgcgctc acctcgagcc gctcccgccg
     5221 ccgcgcggcc cgtccgaaca cgtcatctcg ggccgagtcc cacggcccca cccagggtgg
     5281 ggaaaaaggc tccacccgcc acccgctgtc agcctacggc tgagggtgcc cgtgggccag
     5341 acggccttcg tgccaatctc gtgccggagg cggagaaatt ccgtcacgcc gtctcctccg
     5401 ggactgcgag cgcgacgtgg cgcgcgttgg gcgtgtcctc cggcatgacg ccgatcggct
     5461 cgagcggctc gcgcgcctcg acgatccggt tcgagaggcg cccgatcccc tccacctcga
     5521 cctcgaccgt gtcgccgggc gccacggggc gcgagttcgc cggcgtgccg gagaggaaga
     5581 cgtcgcccgg ctcgagcgtg acgagccgcg agaggtcggc gagcatgtac gcgatcgacc
     5641 agagcagctc gcccgtgttc ccctcctgga cgacctcgcc gttcacgtac gtgcgcagcg
     5701 tgaggttctc cgggttgacg tcggccgcgt ccacgagctc ggggccgatc gggcagaacc
     5761 cgtcctggcc cttgacgcgg agcatcgagc cgcggtcggc gtggcggaag tcgtggacgc
     5821 cgaagtcgtt cgcggccgag tacgcggcca catggtcgag cgcgtcctcg accggcactc
     5881 gcttcatccg cttgccgacg accaccgcga cctcgccctc gtaattcagg taccggcagc
     5941 cgagcgggcg cacgatctcg tcgccgtggc gcgcgagcga ggacggaggc ttgaggaagt
     6001 acgagggatc gggcggcgtg cgcgcggcgt actcctcgac ccggctcgga tagctgaggt
     6061 gcacggcgac gatcttcgac ggcgcgagct ccaccgggtg cctcactcga cgcgcctccc
     6121 gccggtcacg gtgtagccga acgagaacca gaagcccttc ccgacctcgg tcggccggag
     6181 gtcggcgagc tcgtccgccg gagactcgaa gaagcgaagc gtcgcgtcgc cctcccagat
     6241 cggcgagatc tcccggtcct cgccgcccgc ccagacgtgc tcctgcagcg ggtcgccgtc
     6301 cgtctcccac gcggggaaga ggcgggtgtt ccagagcggc ggcgagttga cggtcgggcc
     6361 ctcctcggag gggccggtca gcgtgagcgt cgcctcggcg agccggcggt cgtacgaggc
     6421 gagcgacgcg ccgaagcgcc cgcccggctc gaggcggggc ccggccttgc cgaccgtgac
     6481 cggccgcgtg atccagatcg acccgggctt cttcgggaag ccctgcacga gcccgcgcac
     6541 gaggggcatc ccctgatcga cccacatgaa ggggcagcgc gtgaccgcct cgccgcggta
     6601 ctcggccgcg aggacgacga agaactcctt gtactgcgcg cgcaccgggt cgacgagctc
     6661 gtcgccggac tcggagcacg actgccagtc gacgaagatc gcggcggccg aaccgaggtc
     6721 gtcgcccggc tcgagctccg gcggcagcag ggcgcgggcc gcgtccgggt cggtgcggaa
     6781 ctcgacgatc aggaagtcgc ccgagtagtg ccagggcggt ggcggcacga gcgacgagcg
     6841 gcccgtccgc gtctgcggga gcgaatagcc cgtcaggccg tgcggagtcg tgacgtccac
     6901 gcccgcaacg atatccgcgc gcggcaggcg gctcagccgc cgtcgaagcc ggggtcggcg
     6961 ccgacgacgg tcggctcgac gcgcgcgacc ggctcgccga agaaggcggg gagctcgagg
     7021 aaccgcgtga gcgcctccgg gtcgaggccg ctcgccgcgg agatgtcctc ggcctggaga
     7081 aggacgatcc ccggcgcggg cgcctggccc ggctgtgcga cgccccagtc gaacgtcgcg
     7141 tcggccacgc gctgccacgc gagaaggtac tcgtacgggt tgatcacgcc gtcgtagccg
     7201 agcccgagga gcccgaccgg gtggatctcg aagtggaggt gcggcggcgt cccgacggcg
     7261 tcgccggtcg cgccgacgaa gccgatcacg tcgcccgcgc gcacctgtga gccctggaac
     7321 gcgagcggcg agaacgcgga gaggtgcgcg tagtagtact cgttcccctg ccggtcgcgg
     7381 agccaggcgc gcagaccgcc gacgtcgttc cagccgacga ggaagagcgt cccctcggtg
     7441 accgcgagga ccggcgcgcc gaccggggcg aagatgtcgt ttccgtggtg ccagccggtc
     7501 gtcgcgcgcg acgccgcgaa gtcgtccacg aagctcgacg gcccgtagac cgggaacacg
     7561 aagccctccg aggtcacggc cggccggacc gacgcgggcg ggttctggat gacgggcggc
     7621 gggaccttcg ggaggcccgg cttcggcggc tccgggtcgc gcttcgggcc cttcggcggg
     7681 gcgagcggcg acccggtcgg ctgctcgggc tccgcctcgt cggccggctt gcgcggggcg
     7741 ctcgcggccg cctcggcgta gccgacgagg atctcggcgc cggcgggcag gccgccgtgg
     7801 tcggcggtga ggtacacgtg gaggccggtc acgaaggtcc ggcggccgac gcgcttcggg
     7861 tcccgtacga ccgcctgctc gagcaggatc gcgtagcccc agtcgccgag ctggacgcgg
     7921 gcgttccggc tcgggcggac cgtctcgccg aggatctgca cgcgggcgag ccaggagccc
     7981 gcgaggcttc ccgacgcgcc ggccggacgg gcatccgcgc tcgccttcgt cgagacggcg
     8041 ccgatcgtga tctcgccgcc gaagatcgag accgtacgga gggtcgagct cgcccaggcc
     8101 tcgccggact tcggccgcgt gcttgcccgt gcgcgggccc agacggagcc cgtcgtcacg
     8161 atcccgtcgc cgtagacgag cgagcccgag gtcgcctggc gggcgggcgg cgcgacggcc
     8221 tcggcgagcg cctcggactg cccgtccggg agcacgacgc ggatcgcgag gccgcgggca
     8281 gacgccttcc ccggctcggt tgccgcctgg gccgcgcccg cggccgcgaa ggcagcgagg
     8341 gcgagcacgc tcacgaacgc gagacgcacg cgcatcgcct gaagggtatc gccgtcaccc
     8401 ggcacccgtc gagtactcgg cagcgcgatc ctgcctcgtg agctcagggc cgcggcgagc
     8461 gcgacgccga ccaggcgaga tgcgccgccg tgacgacgag cagcccggcg aagagccgcc
     8521 gaagcgtctc gtcgccgagc gcatcggcga ggagggctcc tccggccacg cccacgccgg
     8581 acgcgagccc ggcgatcgtc gcggcgcgcc agtcgacggc gccgacgcgg tgctggcgcc
     8641 acgccccgag gacgacgacg gggatcatcg cggcgagcga ggtcgcctgc gcgccgagct
     8701 gcgacagccc gacgacgagc gtgagcgtgg gaacgaacag gatcccgccg ccgacgccga
     8761 acgtcccgga gaggacgccg gccgcgaacc cgagcgcgag ggcgaggatg acggtcgtca
     8821 ctcgagcacc agccggatcg cgaccagaac gaggaacccg gcgaagagga acgtgagcat
     8881 ccgcgacgag aggcgccggt gcgcccacgt ccccgcgagc gtgccgccga gcgcgggcag
     8941 cccgacgagc gcggcgtcgc cccaggaaac gtggccgagc gcgccgaacg cgatgacgcc
     9001 gaagaccgcg gtgaacccga tgatcgcgag cgacgtcgcg gtcgcgacct tcacgcggaa
     9061 gccggcgaag atgaggagcg ggatgacgac gatgccgccg ccgacgccga agagcgcccc
     9121 gaagaacccg cccgtgagcc cgatcccgac gagggtcggg gcggacgagg tcaggcgggg
     9181 cacgaccgta tcctaggagc cgcgatggcc gccgacgggt cgttggacgc gctgctcgct
     9241 ccgctcgtcg aggcgccgga gcgctccgcg atcctgctcg acgtggacgg cgtcctcgcg
     9301 ccgatcgtgg cgcgcccgga gct
//
LOCUS       DASTFC010000140        10379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_348511,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000140 DASTFC010000000
VERSION     DASTFC010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348511"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFO88_04215"
     CDS             <1..597
                     /locus_tag="VFO88_04215"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544759.1"
                     /translation="AEGYRFVRSQTWLWGTLLAAAVSLLAFAGPYEVLIPYLVRNELD
                     GGADDLGLVFAAGGAGAVIAALVLGQVGLPRRALTFMYLTWALSVLVLVPYGLATALW
                     HAIVASVLGGAGFTAAIIAWTTLMHRLVPGPVLGRVTSFDWFVSVSLIPVSFAITGPL
                     ADAIGPRTTLVAAGLVSTPLMLAFLFLPGIRDVERGSP"
     gene            594..1880
                     /locus_tag="VFO88_04220"
     CDS             594..1880
                     /locus_tag="VFO88_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544760.1"
                     /translation="MSRLRARASRLGVLRPLAIRDFRLLWAGMSVSLLGDGIYFVAIA
                     WQVYELSNAPTALSVVGVAWTLPNVLLLLVGGVLSDRFDRRRLMIVSDVVRGLAIGTI
                     AVLALSGALELWHLLVLVAVYGAGEALFVPAFQAIVPDIVPQEHLVQANSLDTLMRPL
                     AVQLGGPALGGLAIAAFGTGVAFALDAATFVVSAACLALMRSRPLPAKDAAIGIRSAL
                     REVGEGFAFVRAHRWLWGTLAAAGLSLLLFYGPMEVLLPYVIKNELDGSSQDFGLALA
                     AVGVGAIGAALVMGQRPMPRRPFTVMFTAWAVGMGCVAVWGLATELWQILAVSVLRGV
                     GITVGLVLWTTIMHTRVPRELLGRVSSFDWLMAMSLIPASFAITGPVAEAAGARETLT
                     GAGLLGATLIFFLPFVLGVRRDERVPRPVDVHPDSA"
     gene            1890..2369
                     /locus_tag="VFO88_04225"
     CDS             1890..2369
                     /locus_tag="VFO88_04225"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HET9544761.1"
                     /translation="MPFDVFLRIDGVEGESADAKHKGEIDVLEYDWGLAAPASTRAGG
                     AGAGKVTFQDLRVVARTSKASPTLFLNCATGKAAKEAVLAVRRAGAKQEDYLVVRLKT
                     VRITSYDQTASDEDDTPLDDVSLAFDRIEVEYRAPDPKGALGPPVKAGWDVKQSKKV"
     gene            complement(2374..2727)
                     /locus_tag="VFO88_04230"
     CDS             complement(2374..2727)
                     /locus_tag="VFO88_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544762.1"
                     /translation="MTLKTFGLVSVLASLAIVGYLFLAQSKEVGPTSQAAQQAQADAA
                     AGAAGASFQAAAPVLQAHFAQAGTYAGATLPPSHGVTLVRADGTSYCLQAGTGETARH
                     VVGPAGSPAPGLCPA"
     gene            complement(2770..3099)
                     /locus_tag="VFO88_04235"
     CDS             complement(2770..3099)
                     /locus_tag="VFO88_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544763.1"
                     /translation="MARVNALTDDDRARLAAARSALDEDFREQALRSAAELLVGRAEA
                     YAEARRDVAGAHVPEGLDDDPARRDEVARLVQLAIDELLVGVVVQERLHPKHLRELYR
                     PWRALDG"
     gene            3228..4685
                     /locus_tag="VFO88_04240"
     CDS             3228..4685
                     /locus_tag="VFO88_04240"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HET9544764.1"
                     /translation="MTTLDRRSFLKRGAASAAALMAASGPFAGFANAAKGPKRARANG
                     GYGPLVPVPEVDTGEVLLHLPKGFRYRVLSRIGRPMTDGFPTPARSDGMAAFSWRGGN
                     VRLIRNHEVTFEAPAIGPADKAYDRRSGGGTTTLEVSKDRRLVADWISLNGTNFNCAG
                     GATPWNTWITCEETVNGPDANITFTGATINLERQHGYLFEVPVDRGPGQLEVGDPITA
                     AGRFPHEAVSIDPSSGIVYMTEDDFAWWSGFFRYVPPNNPFRDKRIADGGRLQVLGVV
                     PSGASGPVTTNLSNDQEVGVTYDVAWIDIENPNPTYPTGTPNDPASRVVYQEGEAKGA
                     AQFSRLEGIDYWGGRIYLASTEGGGPFPGGNGSAGFGEGFGQVWVYDTRDETITLLFE
                     SPGKDILDLPDNIVVSPRRKSVLLCEDSTGDNFLRGLTQDGQLFDFALNRWPGAFGDE
                     FAGATFSPDTQTLFVNLQATGVTYAIWGPWERGLL"
     gene            4791..5996
                     /locus_tag="VFO88_04245"
     CDS             4791..5996
                     /locus_tag="VFO88_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9544765.1"
                     /translation="MPDDLQADVLVIGSGASGLAAAVSAERAGARVVLATKGSLQSCN
                     SAKAQGGIQAALGEDDSPEQHAEDVWRSSHETADRSLVEVLTSEAPGAIAWLEELGVE
                     FTRENGGYRLARCGGATRRRLLQVGDRTGLAITTALREAFAAGTATDLPNHALAALDE
                     RPSGWRATLRRKDGVEAAVEAGTVVLAAGGRCYREAKERGELSTNHPGATGEVTEIAR
                     AAGAEARDLDALQYHPNGGTWPANLQGYSIPETTRAYGAVLLNAEREEFTDSLGPRDV
                     VAQAIVDEVAAGRGVETPDGRPAVFLDTTRIPEADALVSLPYMLRRYRAAGIDPLKEP
                     ILTYPVLHYQNGGLVISERGETTVPGLYACGEIAGGTHGRNRMMGNSLLECVVFGRRA
                     GAAAAEVAA"
     gene            5996..6889
                     /locus_tag="VFO88_04250"
     CDS             5996..6889
                     /locus_tag="VFO88_04250"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HET9544766.1"
                     /translation="MAVAVDIQKAVEDAAAHLYVWALKDIPQDLRDAIGAAAERETSV
                     PGRRVLDTIVRNVEIADTGGNLVCQDTGIAVYYCRVGEGFPLHPARIYEALYNGTERA
                     TIEHPLRSNTVHTLTRENTGQNVGYRVPIVHWEFVPDWDGLDVKCVPKGSGSENMSFL
                     KMCVPADGVAGIKRFVLDSIVGAGGKPCPPGIVGVGIGGSADYAMHLAKEAISRPVGT
                     RNEDPLVAELEEELFDLLNATGIGPMGLGGDVTALAVHVEHADTHMTLNPVAVNYQCW
                     AARRATAHLSADGVVDFDREA"
     gene            6889..7473
                     /locus_tag="VFO88_04255"
     CDS             6889..7473
                     /locus_tag="VFO88_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HET9544767.1"
                     /translation="MADVRMTFPTTEEQIRELRAGDSVTVDGHIIGIRDRTQIRIFDQ
                     GQEPPMDLNGAFLLHTAPGVRKLGDGRYEKVCIGTTTSARMVRFTEPLGERYGVRAIC
                     GKGGLPDEAIEPMRRLGMVYFAIVGGAAALETTQIEEIEEVAWEELMPECLWKFRVRD
                     FGPLTVAIDSHGNSLYHDVQAEAKKRLEDLYARL"
     gene            7460..8260
                     /locus_tag="VFO88_04260"
     CDS             7460..8260
                     /locus_tag="VFO88_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HET9544768.1"
                     /translation="MHAFDGPDGFLNLAPRSPKPRTTGLTHVIDKGLNLREIEGLFDT
                     AGAYVDVVKLGWGTSYVTRNLEKKIALYRSFETPVVCGGTLFEAAVALDRLDAYRSWL
                     VENRISHVEVSDGTIELEHERKLELIAELARDFVVMSEVGSKDAEVVFAPYQWVEWMR
                     DELAAGAWKVITEGREGGTAGIFRPTGEMRTGLIDEIVHGIEVGDIIFEAPTKASQAW
                     FVSHLGPEVNLGNIPPEEVIPLETLRLGLRADTLNEILLRERPAPVAD"
     gene            8232..8936
                     /locus_tag="VFO88_04265"
     CDS             8232..8936
                     /locus_tag="VFO88_04265"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET9544769.1"
                     /translation="MSAPPLSLTDAELEALAAEDVVRWSHAEFGERVCLSCSWQKQSS
                     VLVHMLAELGLGIDVIELDTHLFFRETYETRERLVERYGLTLIRPDVLTVAEQHKREG
                     PNLWERDPDRCCHIRKVEPLITALEPYDAWISGIRRDQSPSRANARKVERSERYGVWK
                     IQPLADWSEDDVWRYIVANDIPYNPLHDVGYRSIGCMPCTRPTRPDEEERAGRWAGSD
                     KLECGLHVEAAAEGGR"
     gene            8933..9532
                     /gene="cysC"
                     /locus_tag="VFO88_04270"
     CDS             8933..9532
                     /gene="cysC"
                     /locus_tag="VFO88_04270"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET9544770.1"
                     /translation="MTEHIYGHEHPESGEKREGGFALWFTGLSGSGKTTIAHLVGPEL
                     DARGAIVEYLDGDTVRTHLSKGLGFSKEDRDANIERIGWVASRLVRHGAAVICAAISP
                     YAETRAKARELVEEFGPFVEVYVKASVEECARRDVKGLYEKAFAGEIKGFTGVDDPYE
                     EPEAAEIVIDTEELSPEESAREVVAKLEELGLVPAGATA"
     gene            9529..>10379
                     /gene="sat"
                     /locus_tag="VFO88_04275"
     CDS             9529..>10379
                     /gene="sat"
                     /locus_tag="VFO88_04275"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET9544771.1"
                     /translation="MSTATESQLIAPHGGELVDRTGERPDDLESLPVVRLTPRELADL
                     DMLASGALSPLTGFMGREDYERVVEEMRLANGLAWALPVCLAVDAAPEGDRVALADED
                     GTPIAVLDVEETFEYDKEREAERCFRTTETEHPGVARLFAQADRYLAGPVTVFARQES
                     KFPELHRDPPETRALFAERGWKRVVGFQTRNPIHRAHEYLTKCALETVDGLLIHPLVG
                     ETKSDDVPAAVRVEAYRALVQGYYPEDRVLVSAFPAAMRYAGPREAVWHAICRKNYGC
                     SHFIVGR"
BASE COUNT         1452 a         3702 c         3787 g         1438 t
ORIGIN      
        1 gccgagggct atcgcttcgt ccgctcccag acgtggctct gggggacgct gctcgcggcc
       61 gccgtgtcgc tgctcgcgtt cgccggcccg tacgaggtgc tcatcccgta cctcgtccgg
      121 aacgagctcg acggcggcgc cgacgacctc ggcctcgtgt tcgcggccgg cggcgccggc
      181 gccgtcatcg ccgcgctcgt cctcggccag gtcgggctgc cgcgccgcgc gctcacgttc
      241 atgtacctga cttgggcgct gtccgtcctg gtgctcgtcc cgtacggcct agcgaccgcg
      301 ctctggcacg cgatcgtcgc gagcgtgctc ggcggcgcgg ggttcaccgc cgcgatcatc
      361 gcctggacga cgctcatgca ccggctcgtg cccggccccg tcctcgggcg cgtcacgagc
      421 ttcgactggt tcgtgtccgt gagcctcatc ccggtgtcgt tcgcgatcac ggggccgctc
      481 gcggacgcga tcgggccgcg gacgacgctc gtggccgccg ggctcgtgtc gacgccgctc
      541 atgctcgcgt tcctcttcct ccccgggatc cgcgacgtcg agcggggctc cccgtgagcc
      601 ggctgcgggc gcgggcgtct cggctcggcg tcctgcgccc gctcgcgatc cgcgacttcc
      661 gcctgctctg ggccgggatg agcgtctcgc tcctcgggga cgggatctac ttcgtcgcga
      721 tcgcgtggca ggtgtacgag ctctcgaacg cgccgacggc gctctcggtg gtcggggtgg
      781 cgtggacgct cccgaacgtc ctcctcctgc tcgtcggcgg ggtgctctcc gaccgcttcg
      841 accggcggcg gctcatgatc gtctcggacg tcgtgcgcgg cctcgcgatc gggacgatcg
      901 ccgtgctcgc gctctccggc gcgctcgagc tctggcacct gctcgtgctc gtggcggtct
      961 acggcgcggg cgaggcgctc ttcgtgcccg cgttccaggc gatcgttccg gacatcgtcc
     1021 cgcaggagca cctcgtccag gcgaactcgc tcgacacgct catgcgcccg ctcgcggtgc
     1081 agctcggcgg gccggcgctg ggcgggctcg cgatcgcggc cttcgggacg ggcgtcgcgt
     1141 tcgcgctcga cgcggccacg ttcgtcgtct ccgcggcctg cctggcgctc atgcgctcgc
     1201 ggccgctgcc cgcgaaggac gcggcgatcg ggatccgctc cgcgctccgc gaggtcgggg
     1261 aggggttcgc gttcgtgcgc gcccaccgct ggctctgggg gacgctcgcc gcggccgggc
     1321 tctcgctgct cctcttctac gggccgatgg aggtgctgct cccgtacgtg atcaagaacg
     1381 agctcgacgg gagctcgcag gacttcgggc tcgccctcgc ggcggtcggg gtcggggcga
     1441 tcggcgcggc gctcgtgatg ggccagcggc cgatgccgag gcggccgttc accgtcatgt
     1501 tcaccgcctg ggcggtcggg atgggctgcg tggccgtgtg ggggctcgcg accgagctct
     1561 ggcagatcct cgccgtgtcg gttctccgcg gcgtcgggat cacggtcggg ctcgtcctct
     1621 ggacgacgat catgcacacg cgcgtcccgc gggagctgct cgggcgcgtg tcctcgttcg
     1681 actggctcat ggcgatgagc ctcatccccg cctcgttcgc gatcaccggg ccggtcgccg
     1741 aggccgcggg tgcgcgcgaa accctgaccg gcgccgggct cctcggcgcg acgctcatct
     1801 tcttcctccc gttcgtgctc ggcgtccgcc gcgacgagcg ggtgccgcgc ccggtggacg
     1861 tccacccgga ctccgcgtag catcgcgcca tgccgttcga cgtgttcctg aggatcgacg
     1921 gcgtcgaggg cgagagcgcc gacgcgaagc acaagggcga gatcgacgtg ctcgagtacg
     1981 actgggggct cgccgccccc gcctcgacgc gtgccggggg cgcgggggcc ggcaaggtca
     2041 cgttccagga cctccgtgtc gtcgcgcgga cgagcaaggc ctcgccgacg ctcttcctca
     2101 actgcgcgac cggcaaggcg gccaaggagg cggtgctcgc cgtccggcgg gcgggcgcga
     2161 agcaggagga ctacctcgtc gtcaggctca agacggtgcg gatcacgtcg tacgaccaga
     2221 cggcctccga cgaggacgac acgccgctcg acgacgtctc gctcgccttc gaccggatcg
     2281 aggtcgagta ccgcgcgccg gaccccaagg gcgcgctcgg cccaccggtc aaggccggct
     2341 gggacgtgaa gcagagcaag aaggtctgag ccgctacgcc ggacagagcc cgggcgccgg
     2401 cgacccggcc ggcccgacga cgtgtcgcgc cgtctcgccc gtcccggcct gcaggcagta
     2461 cgacgtgccg tccgcgcgga cgagcgtgac gccgtggctc ggcgggagcg tcgcgcccgc
     2521 gtacgtgccc gcctgcgcga agtgcgcctg gaggacgggg gcggcggcct ggaagctcgc
     2581 gcccgccgcg ccggcggccg cgtccgcctg agcctgctgc gcggcctgcg aggtcggccc
     2641 gacctccttc gactgcgcca gaaacaggta gccgacgatc gcgagcgagg cgaggacgga
     2701 gacgaggccg aaggtcttga gggtcacgcc gcgtgtatcg gccgcttcgc gaggaggctg
     2761 aagcgcggct cagccgtcga gcgcgcgcca ggggcggtag agctcgcgca ggtgcttcgg
     2821 gtgcaggcgc tcctgcacga cgacgccgac gaggagctcg tcgatcgcga gctgcacgag
     2881 ccgggccacc tcgtcccgcc gggccgggtc gtcgtcgagg ccctcgggca cgtgagcgcc
     2941 cgcgacgtcc cggcgcgcct ccgcgtacgc ctccgcgcgg ccgacgagga gctcggcggc
     3001 ggagcgaagc gcctgctcgc ggaagtcctc gtcgagcgcg cttcgcgcgg ccgcgagcct
     3061 cgcccggtcg tcgtccgtca gggcgttcac gcgcgccacg aggccccgga tcgcctcctc
     3121 gctcggcccg ccgaaatcct gcgtttgacc ctcggcaacg atcgggtgag tatacgagcc
     3181 gaccggcgag gctcaggcct cggtcccacc ggagaagagg aggggagatg acgacactcg
     3241 accggaggtc gttcctcaag cgtggagcgg cgtccgcggc ggcgctgatg gccgcctcag
     3301 ggccgttcgc aggcttcgcg aacgccgcca agggcccgaa gcgcgcgcgt gccaacgggg
     3361 gttacgggcc gctcgtcccg gtgccggagg tcgacacggg cgaggtgctc ctgcacctgc
     3421 cgaagggctt ccgctaccgc gtgctgagcc ggatcggcag gccgatgacg gacggcttcc
     3481 cgacccccgc ccggagcgac gggatggccg cgttctcgtg gcgcggcggc aacgtccggc
     3541 tgatcaggaa ccacgaggtg accttcgagg cgccggcgat cgggcccgcc gacaaggcgt
     3601 acgacaggcg ctcgggcggc ggcacgacga cgctcgaggt ctcgaaggac cggaggctcg
     3661 tcgccgactg gatcagcctc aacgggacga acttcaactg cgcgggcggc gcgacgccct
     3721 ggaacacgtg gatcacgtgc gaggagacgg tcaacggccc ggacgcgaac atcacgttca
     3781 cgggcgcgac gatcaacctc gagcgccagc atggctacct cttcgaggtg ccggtcgacc
     3841 gcgggccggg ccagctcgag gtcggcgacc cgatcacggc ggcggggcgg ttcccccacg
     3901 aggccgtctc gatcgacccg tccagcggga tcgtctacat gaccgaggac gacttcgcgt
     3961 ggtggtcggg gttcttccgg tacgtcccgc cgaacaaccc gttccgggac aagcggatcg
     4021 cggacggcgg gcggctccag gtcctcggcg tcgtgccgag cggcgcgagc ggcccggtaa
     4081 ccacgaacct ctcgaacgac caggaggtcg gcgtcacgta cgacgtcgcc tggatcgaca
     4141 tcgagaaccc gaacccgacc tacccgaccg ggacgccgaa cgacccggcc tcccgcgtcg
     4201 tgtaccagga gggcgaggcg aagggcgccg cgcagttcag ccggctcgag ggcatcgact
     4261 actggggcgg gcggatctat ctcgcctcga ccgagggcgg cggcccgttc cccggcggga
     4321 acggcagcgc cggcttcggc gagggcttcg gccaggtgtg ggtgtacgac acccgcgacg
     4381 agacgatcac gctcctgttc gagagcccgg ggaaggacat cctcgacctt ccggacaaca
     4441 tcgtcgtgtc gccgcggcgc aagagcgtcc tcctctgcga ggactcgacc ggcgacaact
     4501 tcctgcgcgg gctgacgcag gacgggcagc tcttcgactt cgcgctcaac cgctggcccg
     4561 gcgcgttcgg cgacgagttc gccggtgcca cgttcagccc ggacacccag acgctcttcg
     4621 tgaacctcca ggcgaccggg gtcacgtacg cgatctgggg cccctgggag cgcgggctgc
     4681 tctaggacgt ccacgcggtg cccggccggt cttcggccgg gcgtccgcgt ggggagtggg
     4741 ataaagaacg cttatcgttt gcattagcga aatctcggta tagtgctgag gtgccggacg
     4801 acctccaggc cgacgtgctc gtgatcggaa gcggggcctc cgggctcgcc gccgccgtgt
     4861 ccgcggaacg cgcgggcgcg cgcgtcgtgc tcgcgacgaa gggctcgctc cagtcgtgca
     4921 actcggcgaa ggcgcagggc gggatccagg cggcgctcgg cgaggacgac tcgcccgagc
     4981 agcatgccga ggacgtctgg cgctcgtcgc acgagacggc cgaccgctcg ctcgtcgagg
     5041 tgctgacctc ggaggcgccg ggcgcgatcg cctggctcga ggagctcggc gtcgagttca
     5101 cgcgcgagaa cggcggctac cggctcgcgc gctgcggcgg cgccacgcgc cggcggctgc
     5161 tccaggtggg cgaccggacg ggcctcgcga tcacgacagc gcttcgcgag gcgttcgccg
     5221 ccgggacggc gaccgacctc ccgaaccacg cgctcgcggc gctcgacgag cggccgtcgg
     5281 gctggcgcgc gacgctgcgc cggaaggacg gcgtcgaggc ggcggtcgag gcgggcaccg
     5341 tcgtgctcgc ggccggcggg cgctgctacc gcgaggcgaa ggagcgcggc gagctctcca
     5401 cgaaccatcc cggcgcgacg ggcgaggtga cggagatcgc acgcgcggcc ggcgcggagg
     5461 cacgcgacct cgacgcgctc cagtaccacc cgaacggcgg cacgtggccc gcgaacctcc
     5521 agggctactc gatccccgag acgacgcgcg cctacggcgc cgtcctcctg aacgcggagc
     5581 gcgaggagtt caccgactcg ctggggccgc gcgacgtggt cgcccaggcg atcgtcgacg
     5641 aggtcgcggc cggccgcggc gtcgagaccc cggacggccg gcccgccgtc ttcctcgaca
     5701 cgacgcgcat ccccgaggcg gacgcgctcg tgtccctccc gtacatgctc cgccgctacc
     5761 gcgcggcggg aatcgacccg ttgaaggagc cgatcctgac ctatcccgtc ctccactacc
     5821 agaacggcgg gctcgtgatc tccgagcgcg gcgagacgac cgtgccgggg ctctacgcct
     5881 gcggcgagat cgcgggcggc acgcacgggc ggaaccgcat gatgggcaac tcgctgctcg
     5941 agtgcgtcgt cttcgggcgc cgcgcgggcg ccgccgcggc agaggtggcc gcgtgatggc
     6001 cgtcgcggtc gacatccaga aggcggtcga ggacgcggcg gcgcacctct acgtctgggc
     6061 gctcaaggac atcccgcagg acctccgcga cgcgatcggc gcggcggccg agcgcgagac
     6121 ctcggtcccg gggcgccgcg tcctcgacac gatcgtgcgc aacgtggaga tcgcggacac
     6181 cggcggcaac ctcgtctgcc aggacaccgg gatcgccgtc tactactgcc gcgtcggcga
     6241 gggcttcccg cttcacccgg cgcggatcta cgaggcgctc tacaacggca ccgagcgcgc
     6301 gacgatcgag catccgctgc gctcgaacac ggtgcacacg ctcacgcgcg agaacacggg
     6361 ccagaacgtc ggctaccggg tgccgatcgt ccactgggag ttcgtccccg actgggacgg
     6421 gctcgacgtg aagtgcgtgc cgaagggctc cggctcggag aacatgagct tcctcaagat
     6481 gtgcgtcccc gccgacgggg tcgcggggat caagcgcttc gtgctcgact cgatcgtcgg
     6541 cgcgggcggc aagccctgcc cgcccgggat cgtcggcgtc gggatcggcg gctcggccga
     6601 ctacgcgatg cacctcgcca aggaggcgat ctcgcgcccg gtggggaccc ggaacgagga
     6661 tccgctcgtc gcggagctcg aggaggagct cttcgacctc ctgaacgcga ccgggatcgg
     6721 cccgatgggg ctcggcggcg acgtgaccgc gctcgcggtg cacgtcgagc acgcggacac
     6781 gcacatgacc ctcaacccgg tcgccgtgaa ctaccagtgc tgggccgccc gccgcgcgac
     6841 cgcgcacctg agcgccgacg gcgtcgtcga cttcgaccgg gaggcgtgat ggccgacgtc
     6901 cgcatgacct tcccgaccac cgaggagcag atccgcgagc tgcgcgccgg cgactcggtc
     6961 acggtcgacg ggcacatcat cgggatccgg gaccggaccc agatccggat cttcgaccag
     7021 ggccaggagc cgccgatgga cctgaacggc gcgttcctcc tgcacacggc gcccggggtc
     7081 cgaaagctcg gcgacggccg ctacgagaag gtctgcatcg ggacgacgac gagcgcgcgc
     7141 atggtgcgct tcaccgagcc gctcggcgag cgctacggcg tccgtgcgat ctgcggcaag
     7201 ggcggcctcc cggacgaggc gatcgagccg atgcgccggc tcgggatggt ctacttcgcg
     7261 atcgtcggcg gcgccgcggc gctcgagacg acccagatcg aggagatcga ggaggtcgcc
     7321 tgggaggagc tcatgcccga gtgcctctgg aagttccgtg tgagggactt cggcccgctg
     7381 accgtcgcga tcgactcgca cggcaactcg ctctaccacg acgtccaggc cgaggcgaag
     7441 aagcgactgg aggatctcta tgcacgcctt tgacgggccc gacgggttcc tgaacctcgc
     7501 cccgcggtcg ccgaagccgc gcacgacggg gctcacgcac gtgatcgaca agggcctcaa
     7561 cctgcgcgag atcgaggggc tcttcgacac ggcgggcgcg tacgtcgacg tcgtgaagct
     7621 cggctggggc acgtcgtacg tgacccggaa cctcgagaag aagatcgcgc tctaccgctc
     7681 gttcgagacg ccggtcgtct gcggcgggac gctcttcgag gccgcggtcg cgctcgaccg
     7741 gctcgacgcg taccgctcgt ggctcgtcga gaaccgcatc tcgcacgtcg aggtctccga
     7801 cgggacgatc gagctcgagc acgagcgcaa gctcgagctg atcgccgagc tcgcccgcga
     7861 cttcgtcgtc atgtccgagg tcggttcgaa ggacgcggag gtcgtgttcg cgccgtacca
     7921 gtgggtcgag tggatgcgcg acgagctcgc cgccggcgcg tggaaggtga tcaccgaggg
     7981 ccgcgagggc ggcaccgccg ggatcttccg cccgaccggc gagatgcgca ccggcctgat
     8041 cgacgagatc gtccacggca tcgaggtcgg cgacatcatc ttcgaggcgc cgacgaaggc
     8101 ctcccaggcg tggttcgtga gccacctcgg gcccgaggtg aacctcggca acatcccgcc
     8161 cgaggaggtc attccgctcg agaccctccg gctcgggctg cgcgccgaca ccctcaacga
     8221 gatcctcctc cgtgagcgcc ccgcccctgt cgctgactga cgccgagctc gaggcgctcg
     8281 ccgcggagga cgtcgtccgc tggtcgcacg ccgagttcgg ggagcgcgtc tgcctctcgt
     8341 gctcctggca gaagcagtcg tccgtgctcg tccacatgct cgccgagctc ggcctcggga
     8401 tcgacgtgat cgagctcgac acgcacctct tcttccgcga gacgtacgag acgcgcgagc
     8461 ggctcgtcga gcgctacggg ctcacgctca tccgccccga cgtgctcacg gtcgccgagc
     8521 agcacaagcg cgagggcccg aacctctggg agcgcgaccc cgaccgctgc tgccacatcc
     8581 ggaaggtcga gccgctgatc acggcgctcg agccgtacga cgcgtggatc tcggggatcc
     8641 ggcgcgacca gtcgccgtcg cgcgcgaacg cccgcaaggt ggagcgctcc gagcgctacg
     8701 gcgtctggaa gatccagccg ctcgcggact ggagcgagga cgacgtctgg cgctacatcg
     8761 tggcgaacga catcccgtac aacccgttgc acgacgtcgg gtaccgttcg atcggctgca
     8821 tgccctgcac gcggccgacg cgccccgacg aggaagagcg agccggccgc tgggcgggct
     8881 ccgacaagct ggagtgtggc ctgcacgtcg aggccgccgc cgaaggagga cgatgacaga
     8941 gcacatctac ggccacgagc accccgagtc cggggagaag cgggagggcg gcttcgccct
     9001 ctggttcacc gggctctccg gctcgggcaa gacgacgatc gcccacctcg tgggcccgga
     9061 gctcgacgcg cgcggcgcga tcgtcgagta cctggacggc gacacggtcc gcacgcacct
     9121 ctcgaagggc ctcgggttct ctaaggagga ccgcgacgcg aacatcgagc gcatcggctg
     9181 ggtcgcctcg cgcctcgtgc gccacggcgc cgccgtcatc tgcgccgcca tctcgcccta
     9241 cgcggagacg cgggcgaagg cgcgcgagct cgtcgaggag ttcggcccgt tcgtcgaggt
     9301 gtacgtgaag gcgtccgtcg aggagtgcgc gcggcgcgac gtgaagggcc tctacgagaa
     9361 ggcgttcgcc ggcgagatca agggcttcac cggggtcgac gacccgtacg aggagccgga
     9421 ggcagcggag atcgtgatcg acaccgagga gctctccccc gaagagagcg cgcgcgaggt
     9481 cgtcgcgaag ctcgaggagc tcggcctcgt cccggccggg gcgaccgcgt gagcacggcg
     9541 acggagagcc agctcatcgc cccgcacggc ggggagctcg tcgaccggac gggcgaacgg
     9601 ccggacgacc tcgagtcgct cccggtcgtg cgcctgacgc cgcgtgagct cgccgacctc
     9661 gacatgcttg cgtccggcgc gctctcgccg ctcacgggct tcatgggccg cgaggactac
     9721 gagcgcgtcg tcgaggagat gcggctcgcg aacggtctcg cgtgggcgct gcccgtgtgc
     9781 ctcgcggtgg acgcggcgcc ggagggcgac cgcgtcgcgc tggccgacga ggacgggacg
     9841 ccgatcgccg tcctcgacgt cgaggagacg ttcgagtacg acaaggagcg ggaggcggag
     9901 cggtgcttcc gcacgaccga gacggagcat cccggcgtcg cgcggctctt cgcgcaggcc
     9961 gaccgctacc tcgccgggcc ggtgacggtc ttcgcgcggc aggagtcgaa gttcccggag
    10021 cttcaccggg acccgcccga gacgcgcgcg ctcttcgcgg agcgcgggtg gaagcgtgtg
    10081 gtcggcttcc agacgcggaa cccgatccac cgcgcccacg agtacctcac gaagtgcgcg
    10141 ctcgagaccg tcgacgggct gctgatccac ccgctcgtcg gcgagacgaa gtccgacgac
    10201 gtccccgccg cggtgcgcgt cgaggcgtac cgcgcgctcg tccagggcta ctacccggag
    10261 gatcgcgtgc tcgtgagcgc gttcccggcc gcgatgcgct acgccggccc gcgggaggcg
    10321 gtctggcacg cgatctgccg caagaactac ggctgctcgc acttcatcgt cggccgcga
//
LOCUS       DASTFC010000141         5916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_925407,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000141 DASTFC010000000
VERSION     DASTFC010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925407"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFO88_04280"
     CDS             <1..507
                     /locus_tag="VFO88_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9544772.1"
                     /translation="VARTVARSFDRDAPVVVPSGSCASMVRDRLPELVGCEPFDVHEL
                     SAFLDANDVPLGETNVGKTIAFHDACHALRELGIVDEPRRLLERTGARLLPLPRPDLC
                     CGFGGTFSVRQPEVSAAMAAEKLAGAAGADALVTGDPGCLLQLNARAKKTGGPPVVHL
                     ATALARGV"
     gene            complement(504..1340)
                     /locus_tag="VFO88_04285"
     CDS             complement(504..1340)
                     /locus_tag="VFO88_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HET9544773.1"
                     /translation="MRPLLAVDGDSLAHRAFHALPSSIKGANGRQSNMLVGFANMLAS
                     LWESERPRTMFVGFDSIGSPTYRHELLPGYQSGRDFPPELTTQLDLLPEWVSALGFPW
                     GKEAGYEADDFVAAAVRVEEDRGGETLVVTSDRDLFQLASDRTTILLPRRGVSELERV
                     GPAEVRERYGVDPVQVPDFIALRGDTADKIPGAKGIGTQRAATILRTHETLDAAIEAG
                     VFREQADDLRLYLRLTRLQYHAPLPELPDAEPDWEGAARLAEEWGLGNLAGRMRERAE
                     SA"
     gene            complement(1337..1966)
                     /locus_tag="VFO88_04290"
     CDS             complement(1337..1966)
                     /locus_tag="VFO88_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9544774.1"
                     /translation="MDGPSAPTRFYSTIVALSRPFMRWPLRLEARGLEHVPERGGFVV
                     ASNHLSNVDPWPVAFPLYPRQVHFMGKAELFRNPALRWFLTNGGAFPVRRGERDAEAF
                     KTAVRLAREGGVVAMFPEGTRRTKGLRKKHVARPHSGAARIALAAGVPLVPAAVAGTD
                     RLGRSPVRVVYGAPLEPPRDGVSRREAGEALTVRLMEEIARLESELAAA"
     gene            2010..4241
                     /locus_tag="VFO88_04295"
     CDS             2010..4241
                     /locus_tag="VFO88_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HET9544775.1"
                     /translation="MSKTLVIAEKPSVGRDLAAALPGAFEKHDEYLESEDYIVTWAIG
                     HLVGLAPPEDYDPKLKKWRFADLPIVPDRFMLIPNDDRAKKQLRVVHKLLKDPRVDRI
                     VNACDAGREGELIFAYVYETSGVDKPVQRLWLNSMTKKAIEEAFEHLRPGEELKLLEA
                     SARSRSEADWLVGMNATRAASIRLRAAFAGAVSLGRVQTPTLALVARREKEIRSFVPE
                     PYWLVEALFAASGEREYRGRYLGGKRIPEDEAATIVDETQGKPGEITSLEKKEEREQP
                     QLLYDLTSLQRNANTLYGFSARRTLAAAQKLYEEHKAITYPRTNSRFLTGELIGEIKP
                     TASLVGANAEYAKAADYVVGLDKLPLGRVVNDKKVTDHHAIIPTRSRHNLGRMGDDER
                     RVYDLVAKRFLAIFHPDAVYERTRVETTVEGHVFRTSGRVLLEAGWKSVYGEEAQRRE
                     SDADDSGGDQLLPVLEQGETVDTREVESIRKETQPPRRFTDASLLAAMETAGKDIADA
                     ELREAMKDSGIGTPATRAAIIERLIQVGYIERDGRALHATDKGVQVIDLLNDHPLTSA
                     ELTGSWEHRLGEIEEGVQDRETFMADIERFTRETVEQLDALKGVKIERANLGPCPVCG
                     RDVIENRKGYSCWTKEDPGCGFVVWKQKAGKTLTAAIVKELMETGRTEKPVSGFKSRA
                     GRQFKAKLKLVQTDEGKWRVDFDEEWAQEPRPEPGADGGNGAGEDGAAPRRGRRKAAA
                     AQE"
     gene            4392..5447
                     /locus_tag="VFO88_04300"
     CDS             4392..5447
                     /locus_tag="VFO88_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9544776.1"
                     /translation="MSDHTAQELLETTQARLLVEGAEERGYIEPAELEALALELDLAE
                     DEVAELTQELEAQGHEIGRPQEAVEETQVTVPEPVIGAGDSLQLFLADVGRHKLLTAS
                     EEVMLAKRIEKGDPLAKRRMIESNLRLVVSIAKGYRGLGVPFLDLIQEGTLGLNRAVE
                     KFDWRRGFKFSTYATWWIRQSVQRAVANNARTIRVPVHVVERQQKLGRAARRLEVELG
                     RDATKEELAEATGLPIQHVDEALGAAQASVSLNQNVGADDEGELGDLFADREAADPFD
                     EAEESLRKQGVRRALDALPERERRILELRFGFEGEPWTLEAIGHELGLTRERVRQLEA
                     QALARLAAIRDLASVAA"
     gene            5579..>5916
                     /locus_tag="VFO88_04305"
     CDS             5579..>5916
                     /locus_tag="VFO88_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9544777.1"
                     /translation="MDAAERKDILNRYLAHARQFDEVAARRESNGQSAEVIPIPIQAP
                     LRELEQEPTMREIEVLQLISEGLVNREIGQRLFLSEETVKSHVRHLLAKLQARSRAHA
                     VAVGFRRGLIA"
BASE COUNT          939 a         2082 c         2119 g          776 t
ORIGIN      
        1 gtcgcgcgga cggttgcccg gtcgttcgac cgggacgctc ccgtcgtcgt gccgtccgga
       61 tcctgcgcgt cgatggtgcg cgaccggctc cccgagctcg tcggctgcga gcccttcgac
      121 gtgcacgagc tctccgcgtt cctcgacgcg aacgacgtcc cgctcggcga gacgaacgtg
      181 ggcaagacga tcgccttcca cgacgcctgc cacgcgcttc gcgagctcgg gatcgtggat
      241 gagccgcgcc ggctcctcga gcgcacgggc gcgcgcctcc tgccgctgcc ccgcccggac
      301 ctctgctgcg gcttcggcgg gacgttctcc gtccgccagc ccgaggtctc ggcggcgatg
      361 gcggccgaga agctcgcggg agcggccggg gcggacgcgc tcgtgacggg cgatcccggg
      421 tgcctcctgc agttaaacgc gcgcgcgaag aagaccggcg gcccgccggt cgtccacctg
      481 gcgaccgccc tcgcgagggg cgtctaggcg gactcggccc gctcgcgcat ccggccggcg
      541 aggttgccga gaccccactc ctcggcgagg cgcgccgcgc cctcccagtc gggctcggcg
      601 tccggaagct ccggcagggg cgcgtggtac tgcagccgcg tgagccgcag gtagagccgg
      661 agatcgtcgg cctgctcccg gaacacgccg gcctcgatcg ccgcgtcgag cgtctcgtgc
      721 gtccggagga tcgtcgccgc ccgctgcgtc ccaatgcctt tcgcaccagg gatcttgtcg
      781 gccgtgtcgc cgcggagcgc gatgaagtcc ggcacctgca ccgggtcaac gccgtagcgc
      841 tcgcgcacct cggccggccc gacgcgctcg agctcgctca ccccgcgacg cgggaggagg
      901 atcgtcgtcc tgtcgctcgc gagctggaag aggtcccggt cgctcgtcac gacgagggtc
      961 tcgccgcccc tgtcctcctc cacccggacc gccgccgcga cgaagtcgtc cgcctcgtag
     1021 cccgcctcct tgccccacgg gaacccgagc gccgagaccc actcggggag caggtcgagc
     1081 tgggtcgtca gctcgggcgg gaagtcgcgc ccgctctggt agccggggag cagctcgtgc
     1141 cggtacgtcg gggagccgat cgagtcgaag ccgacgaaca tcgtccgcgg gcgctcgctc
     1201 tcccagagcg acgcgagcat gttcgcgaag ccgacgagca tgttcgactg ccggccgttc
     1261 gcgcccttga tcgacgaggg cagcgcgtgg aaggcgcggt gggcgagcga gtcgccgtcg
     1321 acggcgagaa gcggcctcac gcggccgcga gctccgactc gagccgcgcg atctcctcca
     1381 tgagccgcac cgtgagcgcc tcgccggcct cgcgccgcga cacgccgtca cggggcggct
     1441 cgagcggcgc tccatacacg acgcggacgg gcgagcgccc gagccggtcg gttccggcga
     1501 ccgccgccgg gacgaggggg acgccggcgg cgagcgcgat ccgtgccgcg cccgagtgcg
     1561 gccgtgcgac gtgcttcttc cggagcccct tcgtgcgccg cgtcccctcg gggaacatcg
     1621 cgacgacgcc gccctcgcgc gcgaggcgaa cggccgtctt gaacgcctcc gcgtcccgct
     1681 cgccgcgccg caccgggaac gcgccgccgt tcgtcagaaa ccagcgcagc gccgggttgc
     1741 ggaagagctc ggccttgccc atgaagtgca cctggcgagg atagagggga aacgcgaccg
     1801 gccacggatc gacgttcgag aggtggttcg acgcgaccac gaagccgccg cgctcgggca
     1861 cgtgctcgag cccccgtgcc tcgagccgaa ggggccagcg catgaacggc cggctgagcg
     1921 cgacgatcgt cgagtagaag cgcgtcgggg cggacgggcc gtccatccgg gccagtatcg
     1981 cccgtcaggc ccttcgctaa ggtgatcacg tgagcaagac actggtgatc gccgagaagc
     2041 cgtccgtcgg tcgcgacctc gcggccgcat tgccgggcgc gttcgagaag cacgacgagt
     2101 acctcgagtc ggaggactac atcgtcacgt gggcgatcgg ccacctcgtc ggcctcgcgc
     2161 cgccggagga ctacgacccg aagctcaaga agtggcgctt cgccgacctg ccgatcgttc
     2221 cggaccggtt catgctgatc ccgaacgacg acagggcgaa gaagcagctc cgcgtcgtgc
     2281 acaagctcct gaaggacccg cgcgtcgacc ggatcgtgaa cgcgtgcgac gccgggcgcg
     2341 agggcgagct catcttcgcg tacgtctacg agacctccgg cgtcgacaag cccgtccagc
     2401 ggctctggct caactcgatg acgaagaagg cgatcgagga ggcgttcgag cacctccggc
     2461 ccggcgagga gctgaagctc ctcgaggcct cggcgcgctc gcgctccgag gcggactggc
     2521 tcgtcgggat gaacgcgacc cgcgcggcct cgatccgcct gcgcgccgcg ttcgccggcg
     2581 cggtctcgct cggccgggtg cagaccccga ccctcgcgct cgtcgcgcgc cgcgagaagg
     2641 agatccggag cttcgtcccc gagccgtact ggctcgtcga ggcgctcttc gccgcgtccg
     2701 gcgagcggga gtaccgcggc cgctacctcg gcggcaagcg aatcccggaa gacgaggcgg
     2761 caacgatcgt cgacgagacg cagggcaagc cgggcgagat cacgagtctc gagaagaagg
     2821 aggagcgcga gcagccgcag ctcctctacg acctcacctc gctccagcgc aacgcgaaca
     2881 cgctctacgg cttctccgcg cgccgcacgc tggccgccgc gcagaagctc tacgaggagc
     2941 acaaggcgat cacgtatccg cgcacgaact cccgcttcct gaccggcgag ctgatcggcg
     3001 agatcaagcc caccgcctcg ctcgtcggcg cgaacgccga atacgcgaag gccgcggact
     3061 acgtcgtcgg cctcgacaag ctcccgctcg ggcgggtcgt gaacgacaag aaggtcaccg
     3121 accaccacgc gatcatcccg acgcgcagcc gtcacaatct cgggcgcatg ggcgacgacg
     3181 agcgccgcgt ctacgaccta gtggcgaagc ggttcctcgc gatcttccat ccggacgccg
     3241 tctacgagcg gacgcgcgtc gagacgacgg tcgagggcca cgtcttccgc acgagcggcc
     3301 gcgtgctgct cgaggcgggg tggaagagcg tctacgggga agaggcgcag cggcgcgaga
     3361 gcgacgcgga cgactcgggc ggcgaccagc tcctgccggt gctcgagcag ggcgagaccg
     3421 tcgacacgcg cgaggtcgag tccattcgca aggagacgca gccgcccagg cgcttcacgg
     3481 acgcgtcgct tctcgccgcg atggagacgg ccgggaagga catcgccgac gcggagctcc
     3541 gcgaggcgat gaaggactcg gggatcggca cgccggcgac acgcgccgcg atcatcgagc
     3601 gcctcatcca ggtcggctac atcgagcgcg acggccgcgc gctccacgcg acggacaagg
     3661 gcgtccaggt gatcgacctc ctgaacgacc atccgctcac gtccgccgag ctgaccggct
     3721 cgtgggagca ccgcctcggc gagatcgagg agggcgtcca ggaccgcgag acgttcatgg
     3781 ccgacatcga gcgcttcacg agggagaccg tcgagcagct cgacgcgctg aagggcgtga
     3841 agatcgagcg cgcgaacctc ggcccgtgcc ccgtctgcgg ccgcgacgtg atcgagaacc
     3901 ggaagggcta ttcctgctgg acgaaggagg atcccggctg cggcttcgtc gtctggaagc
     3961 agaaggcggg gaagacgctg acggccgcga tcgtgaagga gctcatggag acaggccgca
     4021 ccgagaagcc ggtgagcggc ttcaagagcc gtgccggccg ccagttcaag gcgaagctga
     4081 agctcgtcca gacggacgag ggcaagtggc gggtcgactt cgacgaggag tgggcgcagg
     4141 agccacgccc cgagccgggc gcagacggcg ggaacggtgc gggcgaggac ggcgccgcac
     4201 ctcgtcgcgg tcggcgcaag gccgccgccg cgcaggagta acttccggga gcggcggtac
     4261 gttaaacccc ggttaagggg aggctgttcc ctggaacagc cagggaatca gtcgtaggct
     4321 ccgaacgttc aatcgggtgg agcagtctta acccgtgcac ggattttcag cgaaaggaag
     4381 aagagaacac tttgagcgac cacaccgcgc aggagcttct ggagaccacg caggcgcgcc
     4441 tcctcgtcga gggcgccgag gagcgcggct acatcgagcc ggcggagctc gaggcgctcg
     4501 cgctcgagct cgacctcgcc gaggacgagg tggccgagct cacgcaggag ctcgaggcgc
     4561 aaggacatga gatcggccgc ccgcaggagg ccgtggagga gacccaggtc accgttcccg
     4621 agccggtcat cggcgcgggc gacagcctgc agctcttcct cgccgacgtc ggccggcaca
     4681 agctgctgac cgcctccgag gaggtcatgc tcgcgaagcg catcgagaag ggcgacccgc
     4741 tggccaagcg gcggatgatc gagtcgaacc tccggctcgt cgtttcgatc gcgaagggct
     4801 accggggcct cggcgttccg ttcctggatc tgatccagga gggcacgctc ggactgaacc
     4861 gcgccgtcga gaagttcgac tggcgtcgcg ggttcaagtt ctcgacgtac gccacgtggt
     4921 ggatccgcca gtcggtgcag cgtgccgtcg cgaacaacgc gcggacgatc cgcgtgcccg
     4981 tccacgttgt cgagcgccag cagaagctcg gccgggccgc ccgccggctc gaggtcgagc
     5041 tcgggcgcga cgcgacgaag gaggagctcg cggaggcgac cggccttccg atccagcacg
     5101 tcgacgaggc gctcggcgcc gcccaggcgt ccgtgtcgct gaaccagaac gtcggcgcgg
     5161 acgacgaggg tgagctgggc gacctgttcg ccgaccgcga ggccgccgac ccgttcgacg
     5221 aggcggagga gtcgctgcgc aagcagggcg tccgccgggc cctcgacgcg cttcccgagc
     5281 gcgagcggcg gatcctcgag ctgcgcttcg gcttcgaggg cgagccgtgg acgctcgagg
     5341 cgatcggcca cgagctcggc ctcacgcggg agcgggtgcg ccagctcgag gcgcaggcgc
     5401 tcgcccgtct cgctgcgatc cgcgatctcg cctccgtcgc ggcctagcgc cgcattttgc
     5461 agggatttcg agggcggcct ccgggccgcc ctcgcccgtt ccgggaacgt accggggatg
     5521 ggcgcctccc tcgttctagg gtatggccgc ggacccggcg tgccgacgct tcaggcgtat
     5581 ggacgctgcc gagcgcaagg acatcctgaa ccgttatctc gcccacgcgc gccagttcga
     5641 cgaggtcgcg gcgcgccgcg agagcaacgg tcagagcgcc gaggtcatcc cgatcccgat
     5701 ccaggcgccg ctccgcgagc tcgagcagga gccgaccatg cgcgagatcg aggtgctcca
     5761 gctcatctcc gagggcctgg tgaatcgcga gatcggccag cggctcttcc tctcggaaga
     5821 gaccgtgaag tcccacgttc gccatcttct ggcgaagctg caggcgcgga gtcgcgccca
     5881 cgcggtggcc gtcggcttcc gccgcggcct gatcgc
//
LOCUS       DASTFC010000142         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_629014,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000142 DASTFC010000000
VERSION     DASTFC010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629014"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1111
                     /locus_tag="VFO88_04310"
                     /pseudo
     CDS             1..1111
                     /locus_tag="VFO88_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            complement(1455..2558)
                     /locus_tag="VFO88_04315"
     CDS             complement(1455..2558)
                     /locus_tag="VFO88_04315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9544778.1"
                     /translation="MFRLLVRRQRARAAELDERSAVLADRLEELERELADAREESRRA
                     RLVAGLGSSIDLDAVLAQTLETAAGVGPVEAAMVVLPDADGGGPLVATYGMTAEEAAH
                     HPVAGPPPGPDAPAVEIAYRYAAEQAADASLVRGGLVLPLPVAGNEPAGSLAVFWRSA
                     DRAPSDADVAALEALAEGAGPAIANARRFREARKLADLDALTNLHNRRFFHDTLAREV
                     ARAQRYGRRLALVILDIDDFKAINERVGHLAGDSVLAELAERVRAVVRSADIACRVGG
                     DEFAVILPESALADAEQLYRRLQVAVSTRPTGAADRLYLSAGIAELGPGDDAASFFER
                     ADEALFRAKETGKSRVVAAPGESGAGAPATRAS"
BASE COUNT          437 a         1044 c         1094 g          361 t
ORIGIN      
        1 ctcggacggg ctcaaccgct cgctcgaggg ccgggtgctc gccgccgacg tggcgaagcc
       61 gctcaaggac ggcaagccgg gcaagacggt gctccatccg aagggcgagc tcgtcacgtc
      121 cgccgacctg cggcggatcg cggacgagct gcccgagcat gcggagacgt tcaccatccc
      181 cgtccgctcg gtgctcaagt gcaagagcga gttcggcgtg tgccggacgt gctacggcgt
      241 gttcctcgcc accggcgaga actgcgaggt cggcgacgcg gtcgggatca tcgccgcgca
      301 gtcgatcggc gagccgggca cccagctcac gatgcgcacg ttccacacgg gcggcgtcgc
      361 gggcgccgac atcacgcacg gcctcccgcg cgtcgtcgag atcttcgagg cccgcaaccc
      421 gaagggcgcg gccacgctcg cgccgatcgg cggggtcgtg gagatcgagg acaccgagcg
      481 cggcccgaag atcgcaatcg acccgaaggc gctggacgac gagggcgagc cgctgccggt
      541 caaggagttc cagctcccgc gccggacgcg cctgctcgtc gaggccggcc agacggtcga
      601 gccgggcgac ccgctccacg agggctcgct cgccccggcc gacctgctcg cgctccactc
      661 ggccgccggc aaggggtcga cgccgaccga gctctacctc gtcggcgagg tgcagcgcgt
      721 ctacaagtcg cagggcgtcg agatccacga caagcacatc gagctgatcg tccggcagat
      781 gctcaagaag gtccgcatcg agaacgccgg cgacaccgac ctgctgccgg gccagctcgt
      841 ggacaaggtc gtcctcgagc gcgagaacgc ccgcgtgaag aaggcgaagg gcgagcaggc
      901 gacgttcgag ccgctcatcc tcgggatcac gaaggcgtcg ctcgcgacgg agtcgttcct
      961 ctcggccgcc tccttccagg agacgacgaa ggtgctcacc gacgcgtcga tcgagggcaa
     1021 gatcgaccgg ctgctcgggc tcaaggagaa cgtgatcatc gggaagctga tcccggcggc
     1081 cacggggctc aagcgctacc ggaacgtcga gatccgcccg acggagaaga tccccgcggc
     1141 cgcctacacg cggccggcga ccgaggagca gctgcttgca gccctcgagg agatcggctc
     1201 cgacggcgag ggtctcgacc tggccgggct cgacttcgac ttcggcggca cgccgggggc
     1261 ggacggcgcg gcgggcggcg agggcctcga ggcccaggag acgcccgagg tggagagccc
     1321 cctcgacgag gagggctcct cctaggcgct cgtcaccatc ggatcgacat gcggggcggc
     1381 ctcagggccg ccccgttccg tttcgaagcg gcgacgaatc ctccccaaga ggctgtggaa
     1441 gctggggaca actctcagga cgcgcgcgtc gcgggggcgc ccgcgccgct ctcgccgggc
     1501 gcggcgacga cccggctctt acccgtctcc ttggcgcgga agagcgcctc gtcggcgcgc
     1561 tcgaagaacg aggcggcgtc gtcgccgggc ccgagctcgg cgatgccggc ggagaggtag
     1621 aggcggtcgg ccgcgccggt cgggcgcgtc gagaccgcga cctggagccg gcggtagagc
     1681 tgctccgcgt ccgcgagcgc ggactccggc aggatcacgg cgaactcgtc gccgcccacg
     1741 cggcaggcga tgtcggcgct ccgcacgacg gcgcgaacgc gctccgcgag ctcggcgagc
     1801 acggagtcgc ccgcgaggtg gccgacccgc tcgttgatcg ccttgaagtc gtcgatgtcg
     1861 aggatgacga gcgccaggcg gcggccgtac cgctgcgcac gggcgacctc gcgggcgagc
     1921 gtgtcgtgga agaagcgacg gttgtgcagg ttcgtgagcg cgtcgaggtc ggcgagcttg
     1981 cgggcctcgc ggaagcggcg cgcgttcgcg atcgccgggc cggcaccctc ggcgagagcc
     2041 tcgagcgccg ccacgtcggc gtccgatggg gctcggtctg cgctccgcca gaagaccgca
     2101 aggcttcccg ccggctcgtt gccggcgacc ggcagcggga ggacgagccc gccgcggacg
     2161 aggctcgcgt cggccgcctg ctccgcggcg tagcggtagg cgatctcgac ggcgggcgcg
     2221 tccgggccgg gaggcgggcc ggcgacgggg tggtgcgccg cctcctccgc ggtcatcccg
     2281 tacgtcgcga cgagcggtcc gcccccgtcg gcgtcgggga gcacgaccat cgccgcctcg
     2341 acggggccga cgcctgcggc cgtctcgagc gtctgtgcga gcacggcgtc gaggtcgatg
     2401 ctcgagccga gcccggcgac caggcgcgct cggcggctct cctcacgcgc gtccgcgagc
     2461 tcccgctcca gctcttcgag ccggtccgcg agaacggcgc tccgctcgtc gagctcggcg
     2521 gcgcgggcgc gctgccgccg gacgagaagg cggaacacga gcgcgctcgc gacgacggct
     2581 ccggcgccgg ccgcgagcgc agcggcgacc gcgatcgaca gggtggtgtc tgcaaagggc
     2641 ctgatgcgcg cctttccccg gcccgcagac ccggcggcgg cgtgatcacg cagtcgccgc
     2701 gccgggctcc gtccgtagtc ggacaataga acggttcctg ccggatggaa accccacccg
     2761 gcacacggcg caaagccgca tggaggcttt gcggacggcg ccgcgaagct gctaccctac
     2821 gccgaggttc ggagtgggct ccgcccattc cggccttttg ttgcgtctgg aaccccctct
     2881 agaggagacg aagagccacc agtgccgacc gtgaatcagc tcgtccggaa gggccg
//
LOCUS       DASTFC010000143         8761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1106737,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000143 DASTFC010000000
VERSION     DASTFC010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106737"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..840
                     /locus_tag="VFO88_04320"
     CDS             61..840
                     /locus_tag="VFO88_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET9544779.1"
                     /translation="MSRLRARCPDCRTLTAVALDEGYECHSCGRAFAAGLVRVPAAWG
                     EGGEAMVEAAALELPYPETAVIDEDRLADQNLALAAALPDRPLVLGGCCCSHIGAVEG
                     LAARHDRLAVVWFDAHGDLNTPETSPSGNVWGMPFRMLLDSDTVRAEDAVLVGARALD
                     PPEEVFIAESGLHTGDEGITAALAGADAVYVALDVDVLDPDDSVTPFMPEPGGPSVAD
                     VEATLERIRASTPVVGLGLTGVAGVPANVPPLVRFAQAAGL"
     gene            886..1740
                     /gene="accD"
                     /locus_tag="VFO88_04325"
     CDS             886..1740
                     /gene="accD"
                     /locus_tag="VFO88_04325"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET9544780.1"
                     /translation="MEERIEAVIEDRREPKDDGGAKHPNTCPGCGSHYRDDELEATLR
                     VCPQCGHHFPVRARERIAQLADGGTFAEADADLRSADPLGFFDLKAYTERLAQAEMAT
                     GLGDALVAGSASIERRACELAIMDFAFMGGSMGSAVGEKLARACERAAERRVPLVAVT
                     ASGGARMQEGILSLMQMPKTVVSVDLLREARAPFVVVMSHPTTAGVLASFASLGDVIV
                     AEPGALIAFTGPRVVQQTTREKLPDDFGLAESNLRFGHVDAIVRRADLRPYLGRLLAL
                     FVDGRDGR"
     gene            1730..2683
                     /locus_tag="VFO88_04330"
     CDS             1730..2683
                     /locus_tag="VFO88_04330"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET9544781.1"
                     /translation="MAAEPTGPEVEQLRLRERLAKLRGMRLLGGVGVAGELAKLERQI
                     ERIAAEPSADEVWHSVELARHEDRPYTLDYVERMCDDFVELRGDRTFEDNRAIVAGLA
                     RFRGRTVALVGHQKGRTLEERYERSFGMPYPEGYRKAMRVMELADRHGFPLLTLVDTP
                     GAYPGVRAEQHGQGGWIARCQLAMAGLGVPTVACVIGEGGSGGAVAIAVADRVLMQEN
                     AIYSVISPEGCAAILWRDASEAKKAAAAFKPDARHCLELGVIDGIVPEPEGGAHVDHD
                     AAATLLAEGLELALHEVEDEDPAVRRRDRRARFRALGVFTG"
     gene            2970..5288
                     /gene="ligD"
                     /locus_tag="VFO88_04335"
     CDS             2970..5288
                     /gene="ligD"
                     /locus_tag="VFO88_04335"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET9544782.1"
                     /translation="MARLDEYERKRSRAKTPEPFGGKGGRRKAPIFVVQRHDARRLHY
                     DFRLERNGALASWAVPKGVPLEPGERHLAVHVEDHPLEYATFEGEIPAGQYGAGTVEI
                     WDSGTYELVEEKRDGGLTVRLEGARLQGVWTLVPAKLDGDPKNWLLLKKRDGDGIVAG
                     RAYSPMLATLAEGMPSGRGWRYELKWDGYRTLAYLRGGETTLRSRKDQDLTERFASVA
                     RAVPGAVRTNHCVLDGEVCALDDRGRPSFSALQQGGGTLVYYVFDLLELEGRPLVDLP
                     FTERRAELEALLTGSSAHVRLSETFEDGRTLLRVVRDEGLEGLMAKKASSRYEVGRRS
                     RNWLKVKEGLPRQEFVVAGYTKGQGRRSGTFGSLVLAVREGEDLRWVGNVGTGFDERE
                     ITRLLGLLRPLERKTSPFRLPPKMPRVKKGDVVWVTPRLVADVEFAEWTHDRRLRAPR
                     YKGLREDKEPEEVRKERPTSTTIKRGKRELRLTNLDKVFWPEEGITKGDLLEYYRAVA
                     PAVLPHLRDRPFTMKRYPDGIEGGHFFQKDAPKHMPDWIRTRSYRSTSRATREKRTIA
                     YPLVNDELALLWMVNMGCIDLNCWYSRIDRPDRPDFCLFDLDPSPDVGFRETVQVALL
                     VKELLDALGLEGYPKTSGSDGIHVLVPVSRRHTYGDTRRFSEIVAGALARAHPKLVTT
                     EWSKAKRRGVLVDANQNGEGKTIASVYSVRPRPGAPVSTPLRWDEVTTDLDPRDFTFE
                     VVLERIERHGDLFEPVLSGRQSLTAALEALER"
     gene            5401..8229
                     /locus_tag="VFO88_04340"
     CDS             5401..8229
                     /locus_tag="VFO88_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HET9544783.1"
                     /translation="MSSKTPWGVLRLIVAVATVALVATATSAAQTGPPAPNAVATALA
                     YLQTNPGTVGATPESIAELAVTSAYRSTHNGVTHVNIQQQFQGLPVGGAHATVNVADD
                     GDIVFVGGVFQATLEEDEDDLELDAVGAVEAAADELGLDEPERLRVLELSVGNAQESL
                     VSDGGISERPIPASLAWHAGKGGLRLAWQLEIADASDIHYWDATIDAATGDLLGVQDL
                     TIEDEHEDLAGTLSRDASLSASASSATDAGTNNPVIDGSSYEVYEITKESPNDGDRTI
                     VQNPADANASPFGWHDTDAATGPEFTITRGNNAHAYLDQDNNGAPDFDGSPDGGAGLD
                     FSFPIDLTEHAQAYREAVVTNLFYWNNVIHDLTYNYGFTEDAGNFQQTNYRGGPTAGN
                     GDGDYVRAEAADGGGTNNANFNTGLSRMQMYLWPGSTNAFGFQNEVIVDGLGSFPASW
                     ARFGPPAMNAGTSGTLVLVNDGVSTAPVGTPTDGCEPYGPLPAGSIAVVDRANTPPPP
                     PNNQPPTCNFVQQVENAEAAGAVAVIIVNNTTSSTPPVLSGPMTAAPPTIPAVSVNQA
                     DGTTIKAALPIAGTVRKPPTRPGIRDGDIENGIIIHEYGHGISIRTTGGPVVSCLSGN
                     EQAGEGWSDYLAISMLMDPALDDPEGPRGMGPYALFQDSRQGNGIRPRPYSRNWEIQP
                     FSYDSIKSNGWLNGASLALPHGLGHGWAATLWDVTWELVDKHGFNANIYDAWDAGGNN
                     RAFQYVMDGLKLQGCAPTLLRSARAVIAAAGALDDGEDYCTVSAAFARRGFGWSAVDG
                     TTNRNDNDEAFDIDPACRRGFLAPASHAYGTLRTVEAGDAVPLRFNAPEVASRGLDIL
                     AENNPYSRLVNCDTLRTVDTSQTTITPRPAPVPTETPGNSALTKVGSTYHYNWLTEED
                     WAGTCREVVVTRWDGKQHRAFFRFVNES"
     gene            8312..>8761
                     /locus_tag="VFO88_04345"
     CDS             8312..>8761
                     /locus_tag="VFO88_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544784.1"
                     /translation="MSPRSTWTVLALVAALAALALVPSAASAVAEDTSVDRAVSTALA
                     YAQANPDALDAIGADVTDLAVTSAYTSKHNGVTHVNLNQRYAGLEVFGAHATVNVTAA
                     GRVAFVGGGFEANLAAASSAVDLDAAEAVEAAADALDLAEPENLRVLS"
BASE COUNT         1322 a         3181 c         3099 g         1159 t
ORIGIN      
        1 gggatgcgcg agacccaccg gatcgaccgc tgggagaggc cggcgggcta ggctctaggc
       61 gtgtcgcgcc tgcgcgcccg ctgccccgac tgccgcacgc tcaccgccgt cgccctcgac
      121 gagggctacg agtgccactc ctgcgggcgc gccttcgccg ccgggctcgt gcgcgtgccg
      181 gccgcgtggg gcgagggcgg cgaggcgatg gtcgaggccg ccgcgctcga gctcccgtat
      241 ccggagacgg ccgtcatcga cgaggaccgc ctcgccgacc agaacctcgc gctcgccgcg
      301 gcgcttcccg accgcccgct cgtcctcggc ggctgctgct gctcgcacat cggcgccgtg
      361 gaggggctcg cggcgcgcca cgaccgcctc gccgtggtct ggttcgacgc acacggcgac
      421 ctgaacaccc ccgagacgtc gccgagcggg aacgtctggg ggatgccgtt ccggatgctc
      481 ctcgactcgg acacggtccg cgcggaggac gccgtcctcg tgggcgcccg cgcgctcgac
      541 ccgcccgagg aggtcttcat cgccgagagc ggtctccaca ccggcgacga ggggatcacg
      601 gcggcgctcg ccggcgccga cgcggtgtac gtcgcgctcg acgtcgacgt cctcgacccg
      661 gacgactcgg tgaccccgtt catgcccgag ccgggcggcc cgagcgtcgc ggacgtcgag
      721 gcgacgctcg agcggatccg ggcctcgacg ccggtcgtcg ggctcggcct cacgggcgtc
      781 gccggggtgc ccgcgaacgt cccgccgctc gtccgcttcg cccaggcggc cgggctctag
      841 acgcttcgcc ggcccgcccg cccggcggac gtaggatgtg ccgtcatgga ggagcggatc
      901 gaggccgtca tcgaggatcg ccgcgagccg aaggacgacg gcggggcgaa gcacccgaac
      961 acgtgcccgg gctgcggctc ccactaccgc gacgacgagc tcgaggcgac gctccgcgtc
     1021 tgcccgcagt gcggccatca ctttcccgtg cgcgcgcggg agcggatcgc ccagctcgcc
     1081 gacggcggga cgttcgccga ggcggacgcc gacctccgct ccgccgaccc gctcgggttc
     1141 ttcgacctga aggcgtacac cgagcggctc gcgcaggccg agatggcgac cgggctcggc
     1201 gacgcgctcg tcgcgggctc ggcgtcgatc gagcggcgcg cctgcgagct cgcgatcatg
     1261 gacttcgcgt tcatgggcgg ctcgatgggg agcgcggtgg gggagaagct cgcgcgcgcg
     1321 tgcgagcgcg cagccgaacg ccgcgtcccg ctcgtcgccg tgaccgcctc gggcggtgcg
     1381 cggatgcagg aggggattct ctcgctcatg cagatgccga agacggtcgt gtcggtcgac
     1441 ctcctccgcg aggcgcgcgc cccgttcgtc gtcgtcatga gccatccgac gaccgcgggc
     1501 gtcctcgcga gcttcgcgag cctcggcgac gtgatcgtgg ccgagccggg cgcgctcatc
     1561 gcgttcaccg gcccgcgggt cgtgcagcag acgacgcgcg agaagcttcc cgacgacttc
     1621 gggctcgccg aatcgaatct ccgcttcggc cacgtggacg cgatcgtccg ccgcgccgac
     1681 ctgcggccgt acctcggccg gctcctcgcc ctcttcgtgg acggacgcga tggccgctga
     1741 gcccaccggc ccggaggtcg agcagctccg gctgcgggag cggctcgcga agctgcgcgg
     1801 catgcgcctc ctcggcggcg tcggcgtcgc gggcgagctc gcgaagctcg agcgccagat
     1861 cgagcggatc gccgccgagc cgagcgcgga cgaggtctgg cactcggtcg agctcgcgcg
     1921 ccacgaggac cgcccgtaca cgctcgacta cgtcgagcgg atgtgcgacg acttcgtcga
     1981 gctgcgcggc gaccggacgt tcgaggacaa ccgcgcgatc gtcgcgggcc tcgcgcgctt
     2041 ccgcgggcgc accgtcgcgc tcgtcggcca tcagaagggg cgcacgctcg aggagcgcta
     2101 cgagcggagc ttcgggatgc cctatcccga gggctaccgg aaggcgatgc gcgtgatgga
     2161 gctcgccgac cggcacggct tcccgctcct gacgctcgtg gacacgcccg gcgcctatcc
     2221 cggcgtccgc gccgagcagc acgggcaggg cggctggatc gcccggtgcc agctcgcgat
     2281 ggcggggctc ggcgtcccga cggtcgcgtg cgtgatcggc gagggcggct ccggcggcgc
     2341 ggtcgcgatc gcggtcgccg accgcgtgct catgcaggag aacgcgatct acagcgtgat
     2401 atcgcccgag gggtgcgccg cgatcctgtg gcgcgacgcg agcgaggcga agaaggcggc
     2461 ggcggccttc aagccggacg cgcgccactg cctcgagctc ggcgtgatcg acgggatcgt
     2521 ccccgagccg gagggcggcg cgcacgtgga tcacgacgcg gcggcgacgc tcctcgcgga
     2581 ggggctcgag ctggcgctcc acgaggtcga ggacgaggat ccggccgtcc ggcgccgcga
     2641 ccggcgcgcc cgcttccgcg cgctgggtgt ctttaccggt tagtcggcgc aaacccgctg
     2701 gccatgcggg acgcccgcgc gttatgcaca ccttccacag gcttttccgc aggacggaaa
     2761 agcgccggta gcaggtcttt tcggcaggtt tccgcacctc gccgggccgg gccggggatg
     2821 caccgcggca gcacgttaat ggccgaggcc gacgcgaacg tcggccccgg atgccaaccc
     2881 ccccagatcg gcaggtgagt gcttcccact tctgcctgcg gccaaccacg ccgagacaga
     2941 tcgtgcgccg ggcgggcatg ctttcacgcg tggcccggct ggacgagtac gagcgcaagc
     3001 ggtcgcgcgc gaagacgccc gagccgttcg gcgggaaggg cgggcgccgc aaggccccga
     3061 tcttcgttgt ccagcgccac gacgcgcgtc gcctccacta cgacttccgc ctcgagcgga
     3121 acggcgcgct cgcgagctgg gcggtgccga agggagtccc gctcgagccg ggcgagcgcc
     3181 acctcgcggt ccacgtcgag gatcacccgc tcgaatacgc gacgttcgag ggcgagatcc
     3241 cggccggcca gtacggcgcc ggcacggtgg agatctggga cagcggcacg tacgagctcg
     3301 tggaggagaa gcgcgacggc ggcctgaccg tccggctcga gggcgcgcgg ctccagggtg
     3361 tgtggacgct cgtccccgcg aagctcgacg gcgacccgaa gaactggctt ctcctgaaga
     3421 agcgcgacgg cgacgggatc gtggccgggc gtgcgtactc gccgatgctc gcgacgctcg
     3481 ccgaggggat gccgagcggt cgcggctggc gctacgagct caagtgggac ggctaccgca
     3541 cgctcgcgta cctgcgcggc ggcgagacga ccctccgcag ccgcaaggat caggacctga
     3601 ccgagcggtt cgcctccgtc gcgcgcgccg tcccgggcgc cgtccgcacg aaccactgcg
     3661 tcctcgacgg cgaggtctgt gcgctcgacg accgcggccg gccgagcttc tcggcgctcc
     3721 agcagggcgg cgggacgctc gtctactacg tcttcgacct gctcgagctc gaggggcgcc
     3781 cgctcgtcga cctcccgttc accgagcggc gcgccgagct cgaggcgctc ctcaccggct
     3841 caagcgcgca cgtgcgtctc tccgagacgt tcgaggacgg gcgtacgctg cttcgcgtcg
     3901 tccgggacga ggggctcgag gggctcatgg cgaagaaggc ctcgtcccgc tacgaggtgg
     3961 gccggcgctc gaggaactgg ctcaaggtga aggaggggct cccgcgccag gagttcgtcg
     4021 tagccggcta cacgaagggg cagggccgcc gttcgggcac gttcggctcg ctcgtcctcg
     4081 ccgtccgcga gggcgaggac ctccgctggg tcggcaacgt cggcacgggc ttcgacgagc
     4141 gggagatcac gcggctcctc ggcctgctcc gcccgctcga gcgcaagacg agcccgttcc
     4201 gcctgccgcc gaagatgccg cgcgtgaaga agggcgacgt cgtctgggtg acgccgaggc
     4261 tcgtcgccga tgtggagttc gccgagtgga cgcacgaccg ccgcctccgc gctccgcgtt
     4321 acaagggcct ccgagaggac aaggagccgg aagaggtgcg aaaggaacgt cccacgtcca
     4381 cgacgatcaa gcgcggcaag cgcgagctgc gcctgacgaa cctcgacaag gtcttctggc
     4441 cggaggaggg gatcacgaag ggcgacctgc tcgagtacta ccgcgccgtc gctcccgccg
     4501 tcctcccgca cctgcgcgac cggccgttca cgatgaagcg ctatccggac gggatcgagg
     4561 gcggccactt cttccagaag gacgccccga agcacatgcc ggactggatc cggacccgct
     4621 cgtaccgctc gacctcgcgc gcgacgcgcg agaagcggac gatcgcctac ccgctcgtga
     4681 acgacgagct cgccctcctc tggatggtga acatgggctg catcgacctg aactgctggt
     4741 actcgcggat cgacaggccc gaccggccgg acttctgcct cttcgacctc gatccctcgc
     4801 ccgacgtcgg cttccgcgag acggtgcagg tcgcgctcct cgtgaaggag cttctcgacg
     4861 cgctcgggct cgagggctac ccgaagacga gcggctcgga cgggatccac gtgctcgtcc
     4921 cggtctcgcg gcggcacacg tacggggaca cgcgccgctt ctccgagatc gtggccggcg
     4981 cgctcgcccg cgcacatccg aagctcgtga cgacggagtg gtcgaaggcg aagcgccgcg
     5041 gcgtgctcgt ggacgcgaac cagaacggcg aggggaagac gatcgcctcg gtctactcgg
     5101 tccgcccgcg gccgggcgcg cccgtctcga cgccgctccg ctgggacgag gtgacgaccg
     5161 acctcgaccc gcgcgacttc acgttcgagg tcgtgctcga gcggatcgag cggcacggcg
     5221 acctcttcga gcctgtgctc tcaggccgcc agtcgctcac ggccgcgctc gaggcgctcg
     5281 agcgctagcc ggcgcttact tctgcgtcgc tccgcattga cttttgtcgg acgacgcgag
     5341 agactcgccc cggcccgtac ccatggcggg tgcccgaccc acccaaggag gggagacaag
     5401 atgtcgtcca agacgccctg gggcgtcctc cggttgatcg tcgctgtcgc gactgtcgcg
     5461 ctcgtcgcga cagccacgtc ggcggcgcag accggtccgc cagccccgaa cgccgttgcg
     5521 acagcgctcg cgtacctgca gacgaatccc ggcaccgtcg gcgcaacgcc ggagagcatc
     5581 gccgagctcg ccgtgacgag cgcgtaccgc agcacccaca acggcgtcac gcacgtcaac
     5641 atccaacagc agttccaagg cttgcccgtc ggcggcgccc acgcgaccgt caacgtggcc
     5701 gacgacggcg acatcgtctt cgtcggaggc gtcttccagg cgacgctcga ggaggacgag
     5761 gacgacctcg agctcgacgc ggtcggcgcg gtcgaggctg cagccgacga gctcgggctc
     5821 gacgagccgg agcggctccg cgttctcgag ctgagcgtcg gcaacgccca ggagtcgctc
     5881 gtctcggacg gcggcatctc cgagcggccg attcccgcga gcctcgcctg gcacgccggc
     5941 aagggcggtc tgcgcctcgc gtggcagctc gagatcgccg acgcgtcgga tatccactac
     6001 tgggacgcga cgatcgacgc cgcgaccggc gacctcctcg gcgtccagga tctgacgatc
     6061 gaggacgagc acgaggacct ggccggcacc ctctcgcgtg acgcgagcct ttccgcgtcc
     6121 gcctcgagcg cgacggacgc gggcacgaac aaccccgtga tcgacggctc gagctacgag
     6181 gtctacgaga tcacgaagga gagcccgaac gacggcgacc ggacgatcgt ccagaacccg
     6241 gccgacgcga acgcgtcgcc cttcggctgg cacgacaccg acgcggccac cgggcccgag
     6301 ttcacgatca cgcgggggaa caacgcccac gcctacctcg atcaggacaa caacggcgcg
     6361 cccgacttcg acgggagccc ggacggcggg gcagggctcg acttctcgtt cccgatcgac
     6421 ctgaccgagc acgcccaggc gtaccgcgag gcggtcgtca cgaatctctt ctactggaac
     6481 aacgtcatcc acgacctcac ctacaactac gggttcaccg aggacgcggg gaacttccag
     6541 cagacgaact acaggggcgg cccgaccgcc ggaaacggcg acggcgacta cgtccgcgcc
     6601 gaggctgcgg acggcggcgg cacgaacaac gccaacttca acaccggtct ctcgcggatg
     6661 cagatgtacc tctggccggg cagcacgaac gcgttcggct tccagaacga ggtcatcgtc
     6721 gacggcctcg gctcgttccc ggcttcgtgg gcgcgcttcg gcccgccggc gatgaacgca
     6781 ggaacgagcg gcacgctcgt gctcgtgaac gacggtgtct cgacggcccc ggtcgggacg
     6841 ccgaccgacg gctgtgagcc gtatggtccg ttgccggcgg gttcgatcgc ggttgtcgac
     6901 cgcgcgaaca ccccgccgcc gcctccgaac aaccaaccgc ccacctgcaa cttcgtccag
     6961 caggtggaga acgcggaggc cgcgggcgca gtggcggtga tcatcgttaa caacacgacg
     7021 agcagcactc cgccggtcct gagcggcccg atgaccgcgg ctccgccgac catcccggcg
     7081 gtgagcgtca accaggccga cgggacgacg atcaaggcgg cgctccccat cgcgggcacc
     7141 gtgcggaagc cgcccacgcg tcccggcatc cgcgacggtg acatcgagaa cgggatcatc
     7201 atccacgagt acggccacgg gatctcgatc cgcaccacgg gcggccccgt cgtgagctgc
     7261 ctcagcggca acgagcaggc cggcgagggc tggagcgact acctcgcgat ctcgatgctg
     7321 atggacccgg cgctcgacga tccggaaggc ccgcgcggca tgggtccgta cgcgctcttc
     7381 caggacagcc ggcagggcaa cggcatccgt ccgcggccct actcgcgcaa ctgggagatc
     7441 cagccgttct cctacgactc gatcaagtcg aacggctggc tgaacggcgc ctccctcgcc
     7501 ctcccgcacg gcctcggcca cggctgggcg gcgaccctct gggacgtcac ctgggagctc
     7561 gtcgacaagc acggcttcaa cgcgaacatc tacgacgcct gggatgcggg cggaaacaac
     7621 cgcgccttcc agtacgtgat ggacgggctg aagctccagg gctgcgcccc gacgctgctc
     7681 cgcagcgcac gggccgtgat cgcggccgcg ggcgcgctgg acgacggtga ggactactgc
     7741 accgtctccg ctgcgttcgc ccgccgcggc ttcggctgga gcgcggtcga cggcacgacg
     7801 aaccgcaacg acaacgacga ggcgttcgac atcgatccgg cgtgccgccg cggcttcctg
     7861 gccccggcct cgcacgcgta cgggacgctc cggacggtcg aggcgggtga cgcggtgccg
     7921 ctccgcttca acgcaccgga ggtggcgagc cgcgggctgg acatcctcgc ggagaacaac
     7981 ccgtactcgc gcctcgtgaa ctgcgacacg ctgcggacgg tggacacgag ccagaccacc
     8041 atcacgccgc ggcctgctcc ggtcccgacc gagacgccgg gcaactcggc gctcaccaag
     8101 gtcgggagca cgtaccacta caactggctg accgaggagg actgggccgg cacgtgccgc
     8161 gaggtcgtcg tgacccgctg ggacggcaag cagcaccggg ccttcttccg gttcgtcaac
     8221 gagagctaga cgatcagcga ccgacgccag agggcgggcc ttagggcccg ccctcttcgc
     8281 gtttcttcga accgaccgaa ggaggaccgg aatgtcgccc agatccacct ggacggtgct
     8341 cgcactcgtc gccgcgttgg cggcgctcgc gctcgtgccg agcgcagcgt cggccgtcgc
     8401 ggaggacacg tccgtcgacc gggccgtttc gaccgcgctc gcctacgcgc aggcgaaccc
     8461 tgatgcgctc gacgcgatcg gcgcggatgt cacagacctc gcggtgacga gcgcctacac
     8521 gagcaagcac aacggcgtca cccacgtgaa cctgaaccag cggtacgcgg gcctcgaggt
     8581 cttcggggcg cacgcgaccg tgaacgtcac ggccgccggt cgcgtcgcgt tcgtcggcgg
     8641 cggcttcgag gcgaacctcg ccgccgcgtc gtcggcggtc gacctcgacg cggccgaggc
     8701 ggtggaggcc gcggccgacg cgctcgacct cgccgagccg gagaacctgc gcgtcctctc
     8761 g
//
LOCUS       DASTFC010000144         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_876135,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000144 DASTFC010000000
VERSION     DASTFC010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876135"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2922
                     /locus_tag="VFO88_04350"
     CDS             <1..2922
                     /locus_tag="VFO88_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9544785.1"
                     /translation="DLAHRVETVAGGVRVDLVLTARERDRLQAQGIDVAPMRNARGLT
                     VRQQAAAQAAAGFQVFRSYDEQGGIRDELYSIAQRNPSIVKLVVLGRTVQGREIIALK
                     VTKNARTVADGSRPDVLYMGTVHAREWIATEVTRRLLDHFVSRYNRDPEITNLVNTRE
                     LWFMPVANPDGYQFTFDHERLWRKNLRDNNADGQITIGDGVDLNRNYDEGWNYDNEGS
                     SSELSSDTYRGPSAASEPETKAHQALIDRLRFRFLVTYHSYGPLLLYPFGWQVMTPAA
                     DDPLYIAYTGTDARPAVPGFDPGVAADLYMTNGTTDDYSYSKTGALSWTPELEEGCVG
                     CGFVFPDDEALVQAQFEKNLPFALSLARSAPDPSNPQTHLGNTVEPFYLETSSIDPEK
                     SGGPMSDFRFSVSYGDPQPVRVLAKRSIGAVALKYRVNGGPVQSASTSEWEGGERFGD
                     KGDFYYRVVEGAVTGTSPGDTVTVWFEAASNPAIRSDSFDYEARVESSNRVLVLAAED
                     YTGISPVYRRGPLYLSYYGDALAANGIGFDVYDVDANGRTAPSPLGVLGHYDAVVWYT
                     GDDVITRDAGMVPGTASRLANDEMLAVRAYLNEGGRLLYTGKYAGLQYADGYEFDLEA
                     NQPCDPDSGADGCQPLSDDFLQYYLGAYLYNDDAGTTANGKLYDVNGTDSPFGGLSWA
                     FGGPSANNQDHSASFIATSGILPPSTYPQFTSWASAKYDRPGGPFDPHTGSRYAYSQI
                     ADVSYKRLTRTIDLTGSASGNLSFWMSHDTEPDWDYVFVEAHTVGQDDWTTLPDANGN
                     TSQSTGPNDPDLASCPGGWHELHPWLARYQTFDGVGACTPTGTTGEWNAASGRSPGWQ
                     QWSIDLSRYAGSQVEVSISYVSDWSVQGLGVFLDDTVVSTGESTSFEDGFGGWTVAGP
                     PPGSGPNANDWTVTDAGGFPEGAAITTEDSIYLGFGLEGISGPSSRAAVMGRAMGYLL
                     R"
     gene            complement(2980..3435)
                     /locus_tag="VFO88_04355"
     CDS             complement(2980..3435)
                     /locus_tag="VFO88_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544786.1"
                     /translation="MPETNYASGDEFMVEFLGYRFGFNALDFEQRVSAAAVKLELVES
                     RELDPEEAADLVELASEGRIAEPRSLLGDYLVRHWERLALVDDESLVYWLRKLVFRSA
                     WLDHRVKENLLEVSFDETTGDFDYRDPNGGRALLELAPVPSWRPLQFRR"
     gene            3596..5392
                     /gene="recJ"
                     /locus_tag="VFO88_04360"
     CDS             3596..5392
                     /gene="recJ"
                     /locus_tag="VFO88_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET9544787.1"
                     /translation="MSETTASVLVRRGHGEPEAARRFLAAELPGHDPYLLGAMREAVD
                     AVRAAIAGGRRICVHGDYDVDGICATALAVEGLRALGADVEWHLPSRFEEGYGVSGET
                     LARLADEGCGLLLTVDCGITAVAEVAEARARGLEVVVTDHHRPGETLPDCPVVAPRPS
                     DYPFPELCGTGVVYKLLEALEAPELDRRLDLVALATVADVVPLLDENRALVTAGLRRL
                     ARTTRPGLRALMRVAHVDPARVDAGALGFRLGPRINAAGRLGHPGAALELLLTDDPAE
                     AERLAGELERLNRDRQAVEDRILRDALRQVSEWPESARRRRGYVLAGEDWHRGVIGIV
                     ASRLAERLGRPVVLLAPADEGWVGSGRSIPAFDLHAALGACAEHLTRWGGHRAAAGLS
                     LDDESRDAFARAFAAHADAVLDDAALAETLVVDAVVDGRELTLDLCEELGRLAPFGLG
                     NPGVTLLAAGCELGELAAVGEGKHLRLAVTANGARSGAIAFGWGERLDRYRRPGRYDV
                     AFRLDANHWNGTVAPQLVVRRIFETPERYEDLRRALAAEWRAGPDAWSPWAREVFEEL
                     ELVGAPSGARRSLAESAAFRAALLAEPVPAAA"
     gene            5443..>5903
                     /locus_tag="VFO88_04365"
     CDS             5443..>5903
                     /locus_tag="VFO88_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9544788.1"
                     /translation="MSQVVHAGSVGRHEHELEALLEGVAAYNPEVDRELLTRALAFAA
                     NAHEGQQRRSGEEFVAHPLGVARILTDLHADDQTLAAALLHDVVEDTRRSIDEVRAEF
                     GDEIAQLVEGVTKLTRIHFQSREHAQAENYRKMVLAMAQDERVILIKLADRL"
BASE COUNT          842 a         2149 c         2135 g          777 t
ORIGIN      
        1 gacctcgcgc acagggtcga gacggtcgcg ggcggcgtcc gcgtcgatct cgtcctgacc
       61 gcgcgggagc gcgaccgcct ccaggcgcag ggcatcgacg tggcgccgat gcggaacgcc
      121 cgcggcctga ccgtccgtca gcaggcggcc gcgcaggcgg cggccggctt ccaggtcttc
      181 cgctcgtacg acgagcaggg cgggatccgc gacgagctct actcgatcgc gcagcgcaac
      241 ccgtcgatcg tgaagctcgt cgtgctcggg cggacggtgc agggccgcga gatcatcgcg
      301 ctcaaggtga cgaagaacgc acgcaccgtc gcggacggct cgcgcccgga cgtcctctac
      361 atgggcaccg tccacgcgcg cgagtggatc gccaccgagg tgacgcggcg cctgctcgac
      421 cacttcgtct cgcgctacaa cagggacccg gagatcacga acctcgtgaa cacgcgcgag
      481 ctctggttca tgccggtcgc gaacccggac ggctaccagt tcacgttcga ccacgagcgg
      541 ctctggcgga agaacctccg cgacaacaac gcggacggcc agatcacgat cggggacggc
      601 gtcgacctga accggaacta cgacgagggc tggaactacg acaacgaggg atcctcgtcc
      661 gagctctcgt cggacacgta ccgcggcccg tctgccgcgt ccgagcccga gacgaaggcg
      721 caccaggcgc tgatcgacag gctccgcttc aggttcctcg tcacgtacca ctcgtacggg
      781 ccgctcctgc tctatccgtt cggctggcag gtcatgacgc cggcggccga cgacccgctc
      841 tacatcgcgt acacgggcac ggatgcccgg ccggccgtcc cgggcttcga ccccggtgtg
      901 gcggccgacc tgtacatgac gaacgggacg accgacgact actcgtactc gaagacgggc
      961 gcgctgagct ggacgcctga gctcgaggaa ggctgcgtcg ggtgcgggtt cgtcttcccg
     1021 gacgacgagg cgctcgtgca ggcgcaattc gagaagaacc tgccgttcgc gctttcgctc
     1081 gcccgctcgg cgccggaccc gtcgaacccg cagacgcatc tcgggaacac ggtggagccg
     1141 ttctacctcg agacgtcgag catcgacccg gagaagtccg gcgggcccat gagcgacttc
     1201 cggttcagcg tctcgtacgg cgacccgcag ccggtccgcg tgctcgcgaa gcggtcgatc
     1261 ggcgccgtcg cgctcaagta ccgcgtgaac ggcggcccgg tgcagagcgc ctccacctcg
     1321 gagtgggagg gcggcgagcg cttcggcgac aagggcgact tctactaccg cgtcgtcgag
     1381 ggcgccgtga cgggcacgag ccccggcgac accgtgaccg tctggttcga ggccgcctcg
     1441 aacccggcga tccggagcga ctcgttcgac tacgaggcga gggtcgagtc ctcgaaccgc
     1501 gtgctcgtcc tcgcggcgga ggactacacc gggatctccc cggtctaccg gcgtgggccg
     1561 ctgtacctct cgtactacgg cgacgcgctc gcggcgaacg ggatcggatt cgacgtgtac
     1621 gacgtcgacg cgaacggccg cacggctccg agcccgctcg gcgtgctcgg ccactacgac
     1681 gcggtcgtct ggtacacggg tgacgacgtg atcacccgtg atgcgggcat ggttcccggc
     1741 acggcgtcgc ggctcgcgaa cgacgagatg ctcgcggttc gcgcctacct gaacgagggc
     1801 ggacgcctgc tctacacggg caagtacgcc ggcctgcagt acgcggacgg gtacgagttc
     1861 gacctcgagg cgaaccagcc gtgcgacccg gacagcgggg cggacggctg ccagccgctc
     1921 tcggacgact tcctccagta ctacctgggg gcgtacctct acaacgacga cgccgggacg
     1981 acggcgaacg ggaagctcta cgacgtgaac ggcacggaca gcccgttcgg cggcctctcg
     2041 tgggcgttcg gtgggccgag cgcgaacaac caggaccaca gcgcgtcgtt catcgcgacg
     2101 agcgggatcc tgccgccctc gacctacccg cagttcacga gctgggcctc ggcgaagtac
     2161 gaccggcccg gcgggccgtt cgacccgcac accgggtcgc gctacgccta ctcgcagatc
     2221 gccgacgtgt cgtacaagcg gctgacgcgg acgatcgacc tgacgggctc ggcgagcggc
     2281 aacctctcgt tctggatgtc gcacgacacc gagcccgact gggactacgt cttcgtcgag
     2341 gcgcacacgg tcggccagga cgactggacg accctgccgg acgcgaacgg gaacacgagc
     2401 cagagcaccg gcccgaacga tcccgacctc gcgagctgcc cgggcgggtg gcacgagctg
     2461 cacccgtggc tcgcccgcta ccagacgttc gacggcgtgg gcgcgtgcac cccgacgggg
     2521 acgaccggcg agtggaacgc cgcctcgggc cgctctcccg gctggcagca atggtcgatc
     2581 gacctctccc gctacgccgg cagtcaggtc gaggtgtcga tctcgtacgt gagcgactgg
     2641 tccgtccagg gcctcggcgt cttcctcgac gacaccgtcg tctcgaccgg cgagagcacc
     2701 tcgttcgagg acggcttcgg cggctggacg gtggcggggc cgccgccggg cagcgggccg
     2761 aacgcgaacg actggaccgt gaccgatgcg ggcggcttcc ccgagggggc cgcgatcacg
     2821 accgaggact cgatctacct cggcttcggg ctggagggaa tctcgggccc gagcagcagg
     2881 gccgcggtca tgggccgcgc gatgggctac ctgctccgct agctcggacg cggtatgggg
     2941 agtgcgagag gggcggccgt cgggccgccc ctctcggctc tagcggcgga actggagcgg
     3001 gcgccaggac ggcaccggcg cgagctcgag gagcgcgcgc ccgccgttcg ggtcgcggta
     3061 gtcgaagtcg cccgtcgtct cgtcgaacga gacctcgagg aggttctcct tcacccggtg
     3121 gtcgagccag gcgctccgga agacgagctt gcggagccag tagaccagcg actcgtcgtc
     3181 cacgagcgcg aggcgctccc agtgccggac gaggtagtcg ccgagcaggc tgcgcggctc
     3241 cgcgatccgc ccctcggagg cgagctcgac gaggtcggcg gcctcctccg ggtcgagctc
     3301 gcgcgactcg acgagctcga gcttgacggc cgccgcgctc acccgctgct cgaagtcgag
     3361 cgcgttgaag ccgaagcggt agccgaggaa ctcgaccatg aactcgtcgc cggaagcgta
     3421 gttcgtctcg ggcattcgtc tgaggataac ccggccggcg gcgacgggcg tcccccgccg
     3481 cgcgaggagg cgccgtccgg cccgcccgcc attctgagtt gccatgcagg agggcacgtg
     3541 gacgatccgt ccgtgccccc gcggcgaggt caggtcgctg gcgtcggccc ttgacgtgag
     3601 cgagacgaca gcgagcgtcc tcgtccggcg gggccacggc gaaccggagg ccgcgcggcg
     3661 gtttctcgcc gccgagctcc cgggccacga cccgtacctg ctcggcgcga tgcgggaggc
     3721 ggtggacgcc gtgcgggcgg cgatcgcggg cggccggcgg atctgcgtgc acggcgacta
     3781 cgacgtggac gggatctgcg cgaccgcgct cgccgtcgag ggcctgcgcg cgctcggcgc
     3841 ggacgtcgag tggcacctcc cgagccgctt cgaggagggg tacggcgtga gcggcgagac
     3901 gctcgcccgc ctcgcggacg agggctgcgg gctgctcctc accgtcgact gcgggatcac
     3961 ggcggtcgcc gaggtcgccg aggcgcgcgc acgcgggctc gaggtcgtcg tgacggacca
     4021 ccaccgcccc ggcgagacgc tcccggactg cccggtcgtc gcgccgcgcc cgtcggacta
     4081 cccgttcccc gagctctgcg ggaccggtgt cgtctacaag ctgctcgagg cgctcgaggc
     4141 cccggagctc gaccgccggc tcgacctcgt cgcgctggcg acggtcgccg acgtcgtccc
     4201 gcttctcgac gagaaccgcg cgctcgtgac cgcaggcctt cgccggctcg cgcggacgac
     4261 ccggccgggc ctccgcgccc tcatgcgcgt cgcgcacgtc gacccggcgc gcgtcgacgc
     4321 gggcgcgctc ggcttccggc tcggtccgcg gatcaacgcg gcgggccggc tcggccatcc
     4381 gggcgccgcg ctcgagctcc tgctcaccga cgacccggcc gaggccgagc gcctcgcggg
     4441 cgagctcgag cggctgaacc gcgaccggca ggcggtcgag gaccggatcc tccgcgacgc
     4501 gctccggcag gtctcggaat ggccggagag cgcgcgccgg cggcgcgggt acgtgctcgc
     4561 gggcgaggac tggcaccgcg gcgtgatcgg gatcgtcgcc tcacggctcg cggagcgcct
     4621 cggccggccg gtcgtcctgc tcgcgcccgc ggacgagggc tgggtcggct ccggccgctc
     4681 gatccccgcg ttcgacctgc acgccgcgct cggcgcgtgc gccgagcacc tgacgcgctg
     4741 gggcggccat cgcgcggccg cagggctctc cctcgacgac gagagccgcg acgcgttcgc
     4801 gcgcgccttc gccgcgcacg ccgacgccgt gctggacgac gccgcgctcg ccgagacgct
     4861 cgtcgtcgac gcggtcgtgg acggccgcga gctcacgctc gacctctgcg aggagctcgg
     4921 ccggctcgcc ccgttcggcc tcgggaaccc gggcgtcacg ctcctcgccg ccggctgcga
     4981 gctcggtgag ctcgctgcgg tgggggaggg gaagcacctc cgcctcgcgg tcaccgcgaa
     5041 cggcgcgcgc tcgggcgcga tcgccttcgg ctggggcgag cggctcgacc gctaccgccg
     5101 cccgggacgc tacgacgtcg ccttccggct cgacgcgaac cactggaacg gcaccgtcgc
     5161 gccccagctc gtcgtccggc ggatcttcga gacgcccgag cgctacgagg acctgcgccg
     5221 cgcgctcgcc gccgaatggc gcgcgggccc ggacgcgtgg agcccctggg cccgcgaggt
     5281 cttcgaggag ctcgagctcg tcggagcccc gtccggcgcc cgccgcagcc tcgccgagag
     5341 cgccgcgttc cgggccgcgc ttctcgccga gccggttccc gccgcggcct gagcggaacg
     5401 gggccggggt tcgtccggca ggcccggagc tacactgagc gtatgagtca ggtcgttcac
     5461 gctggctcgg tcgggcggca cgagcacgag ctcgaggcgc tgctcgaagg ggtcgcggcc
     5521 tacaacccgg aggtcgaccg ggagctgctc acgcgggcgc tcgccttcgc ggccaacgcg
     5581 cacgagggcc agcagcgccg gagcggcgag gagttcgtcg cccatccgct cggggtcgcg
     5641 cgcatcctca cagacctcca cgccgacgac cagaccctcg ccgcggcgct cctccacgac
     5701 gtcgtcgagg acacgcggcg ctcgatcgac gaggtgcgcg cggagttcgg cgacgagatc
     5761 gcccagctcg tcgagggcgt cacgaagctg acccggatcc acttccagag ccgcgagcac
     5821 gcgcaggccg agaactaccg gaagatggtc ctcgcgatgg cgcaggacga gcgcgtcatc
     5881 ctgatcaagc tcgccgaccg cct
//
LOCUS       DASTFC010000145         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_464070,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000145 DASTFC010000000
VERSION     DASTFC010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464070"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFO88_04370"
     CDS             <1..250
                     /locus_tag="VFO88_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544789.1"
                     /translation="LIMFGVLGFIPGYLMAKLVNAFGLLRVPASVEAAGLDAHDVGDV
                     YPYLPQRDTLFEIAEREAALEAVRAGNGRGAAREEVRR"
     gene            247..621
                     /locus_tag="VFO88_04375"
     CDS             247..621
                     /locus_tag="VFO88_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544790.1"
                     /translation="MTVLGFSTGLDSFSNPTEIGPLYPFVGAEWFFVLLAVVLWLLWH
                     VLQIRLENRDNERAVQTYERIGLDRVMFYGGSADVPSDEEWARREEQRRRVQTPSAGE
                     PSSDLDDPVEKLPPVPPGRNDG"
     gene            complement(628..1431)
                     /locus_tag="VFO88_04380"
     CDS             complement(628..1431)
                     /locus_tag="VFO88_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET9544791.1"
                     /translation="MARTGQPSARRIAAVERAIGLLEALERGGEHGTNELARTTGLSP
                     STVSRVLATLAAGGLVEHVPATGRYRLGLRLVELGGAALAGTDLREVARPHLQALVRA
                     TGETATLSAPAEGRAITVDFVQSGASVQSVARLGRPSVAHATATGKVLLAFGSVAELP
                     PEPLERFTARTTTSRADLARELERARAHGYAEAAGEREEDLNAIAAPVRGVHGELVAI
                     LGVQGPAGRFAPAAMEAAVPALLERAAALSRTLGHRTEGPGTARAAPGH"
     gene            1493..>2472
                     /locus_tag="VFO88_04385"
     CDS             1493..>2472
                     /locus_tag="VFO88_04385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9544792.1"
                     /translation="MTDLSNARYVVVGAGMHGLSTAYHLARELEARGLGQGSDVLVLE
                     KSEPGAGATGIACGVVRNNYFQPAMSELMQACVEVWESDPEGYAYQPVGYIALGAASQ
                     EADLVATYERQERIGYRSRIVTGEAEVAEYMRGLFPDWRARGVTVCLHELQGGFAFNR
                     ESVAGLARKCEAAGVRIATGVEVTGLDSAGDGSVRAVETSAGRIEVGEQVVVAPGPWA
                     QDFWRMLDLPPTIDIRLPSGEVERDRPMWTFWNLQEGEITVDPTLFALADGGAPPVIH
                     LDSDAPLHTDEGKLVTDELWGIYFKRDREGVQGGASPLVVDGDVELDPYPL"
BASE COUNT          316 a          885 c          941 g          330 t
ORIGIN      
        1 gctcatcatg ttcggcgtgc tcgggttcat cccgggctac ctgatggcga agctcgtgaa
       61 cgccttcggg ctcctgcgcg tgcccgcctc ggtcgaggcg gccgggctcg acgcccatga
      121 cgtcggcgac gtgtacccgt atctcccgca gcgcgacacg ctgttcgaga tcgccgagcg
      181 cgaggcggcc ctcgaggccg tccgggccgg caacgggcgc ggagccgcga gagaggaggt
      241 caggcgatga cggtcctcgg cttcagcacc gggctcgact cgttctccaa cccgacggag
      301 atcgggcccc tgtacccgtt cgtgggcgcg gagtggttct tcgtgctcct cgcggtcgtc
      361 ctctggctgc tctggcacgt gctccagatc cggctcgaga accgcgacaa cgagcgggcc
      421 gtgcagacgt acgagcgcat cgggctcgac cgcgtgatgt tctacggggg cagcgcggac
      481 gtcccgagcg acgaggagtg ggcgcgccgc gaggagcagc ggcggcgcgt ccagacgccc
      541 tccgccgggg agccctcgtc cgacctggac gaccccgtgg agaagttgcc tccggttccg
      601 cccgggcgca acgacggcta ggaacggtca gtggccgggg gcggcgcgcg ccgtccccgg
      661 cccctccgtg cggtgtccca gggtgcgcga gagcgccgcg gcgcgctcga ggagcgccgg
      721 gacggcggcc tccatcgcgg cgggcgcgaa gcggccggcc gggccctgga cgccgaggat
      781 cgccacgagc tcgccgtgga cgccgcgcac cggggcggcg atcgcgttca ggtcctcctc
      841 ccgctcgccg gcggcctcgg cgtagccgtg cgcgcgcgcc cgctcgagct cgcgcgcgag
      901 gtcggcccga ctcgttgtcg tgcgcgcggt gaagcgctcg agcggctccg gtggcagctc
      961 ggcgaccgac ccgaacgcca ggagcacctt gccggtcgcg gtggcgtggg cgacgctcgg
     1021 gcggccgagc cgcgcgacgc tctggaccga cgccccgctc tggacgaagt cgacggtgat
     1081 cgcccggccc tccgccgggg cggagagcgt cgccgtctcg cccgtcgcgc ggacgagcgc
     1141 ctggaggtgc gggcgggcga cctcccgcag gtcggtgccg gcgagcgcgg cgcctcctag
     1201 ctcgacgagc cggaggccga ggcgatagcg gcccgtcgcc gggacatgct ccacgagccc
     1261 accggccgcg agggtcgcga ggacgcggga gaccgtgctc gggctgaggc cggtcgtccg
     1321 cgcgagctcg ttcgtgccgt gttcgccgcc gcgctcgagc gcctcgagga ggccgatcgc
     1381 ccgctcgacg gcggcgatgc ggcgggccga cggttgaccg gttctcgcca tgcgggtacg
     1441 ctcgacacga attctggcat gccatttcca ccagcgcaat aaggagctcg acgtgaccga
     1501 cctctcgaat gcccgctacg tcgtcgtcgg cgccgggatg cacggcctct cgaccgccta
     1561 tcacctggcc cgcgagctcg aggcccgcgg cctcgggcag gggagcgacg tcctcgtgct
     1621 cgagaagagc gagccgggcg ccggcgcgac cgggatcgcg tgcggcgtcg tccgcaacaa
     1681 ctacttccag ccggccatgt ccgagctcat gcaggcgtgc gtcgaggtct gggagtccga
     1741 ccccgagggg tacgcgtacc agccggtcgg ctacatcgcg ctcggcgcgg cgagccagga
     1801 agccgacctc gtggcgacgt acgagcgcca ggagcggatc ggctaccgct cgcggatcgt
     1861 caccggcgag gccgaggtgg ccgagtacat gcgcgggctc ttccccgact ggcgcgcccg
     1921 cggcgtgacc gtctgcctgc acgagctcca gggcgggttc gccttcaacc gggagtcggt
     1981 cgccgggctc gcacgcaagt gcgaggcggc gggcgtccgg atcgcgaccg gcgtcgaggt
     2041 gaccgggctc gactcggcgg gcgacggctc ggtccgcgcg gtcgagacgt cggcgggccg
     2101 catcgaggtc ggcgagcagg tggtcgtcgc gccggggccg tgggcgcagg acttctggag
     2161 gatgctcgac ctgccgccca cgatcgacat ccgcctcccg tcgggcgagg tcgagcgcga
     2221 ccggcccatg tggacgttct ggaacctcca ggagggtgag atcaccgtcg acccgacgct
     2281 gttcgcgctc gccgacggcg gggcaccgcc cgtcatccac ctggacagcg acgcgccgct
     2341 ccacaccgac gaggggaagc tcgtgaccga cgagctctgg gggatctact tcaagcgtga
     2401 ccgcgagggc gtgcagggcg gcgcctcgcc gctcgtcgtg gacggggacg tcgagctcga
     2461 cccgtacccg ct
//
LOCUS       DASTFC010000146         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_68675,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000146 DASTFC010000000
VERSION     DASTFC010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68675"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFO88_04390"
     CDS             complement(<1..839)
                     /locus_tag="VFO88_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544793.1"
                     /translation="MSLLLLAFVVMVVVMALMSMSARRMRRNALEEEARRREELRRHA
                     GNGESPQPELPFPFNLFFGEAQSYTYDFETGQWVEITDVSPEPEPEAEAVPERTERER
                     DERPGRPRRPRRTRRRPAQQSSPLGGLLGGGMMGGLGGGSGNFDVQPPEELTTFEDVG
                     GMEALKQEIRDTVGLVLAHPDDAERYGIDWNGILLHGPPGVGKTFFARAIAGEHGLNM
                     IHVSTGDLVGSLVGQSAQNIDKAFQTALDNLPCLLFFDEFDSVAQRRDSTPDQESRRT
                     VNQL"
     gene            complement(901..1653)
                     /locus_tag="VFO88_04395"
     CDS             complement(901..1653)
                     /locus_tag="VFO88_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HET9544794.1"
                     /translation="MSRRRVALVAAVGALVAAPAAHAHFAFAPGEVPARSFARLQLQV
                     PNEEPNASTVRVAVQVPDSVLFVRVPPTPGWQVNVEREPLDEPLLVGDRRVDERITVI
                     EWSGGEITGSNSQSFALRLAVPRAAGQTLAFQATQTYSDDQVVEWIGEPGSENPAPLL
                     TVTPFDPSAPSLAIRPAPAEEPPPPPPTTTEEESETESEEEETEDTETTAVQASDEDD
                     DGVSAPLLVALALGIAAAFAAGIWLMARRRAG"
     gene            complement(1682..>2609)
                     /locus_tag="VFO88_04400"
     CDS             complement(1682..>2609)
                     /locus_tag="VFO88_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9544795.1"
                     /translation="AVAALAAGLDAAIPAKVVDRNLLIGTWNVQALSRVTKRWTPRED
                     DSPKRNLQDVWCIAEVVSRFDVVALVEIRHTLEALRLLMELLGPNWSFLVTDTVVGKP
                     GNDERLGFLFDTRRVQLTGMAGELVIPDEELQGADAIMRRQFARTPYAVSFRAGRDAF
                     TLVSLHVLWGKKAADRTPELLRIARWLSEASTDPEDFNRNLIALGDFNIDRYDDPNWR
                     AFVVEYGLSPPAALFDVPRTLADTEGDTSYFDQIAWFTTGDRSALTLEYSGRAGGFLW
                     TDHILQDVPRRRKEARISDHYPLWAEFRLTGA"
BASE COUNT          351 a          932 c          958 g          368 t
ORIGIN      
        1 agctggttga cggtgcggcg cgactcctgg tccggtgtcg agtcgcgccg ctgggcgacc
       61 gagtcgaact cgtcgaagaa gaggaggcac gggagattgt cgagcgccgt ctggaacgcc
      121 ttgtcgatgt tctgcgccga ctggccgacg agcgagccga cgaggtcgcc ggtcgagacg
      181 tggatcatgt tcaggccgtg ctcgccggcg atcgcgcgcg cgaagaacgt cttccccacg
      241 ccgggcggcc cgtgcagcag gatcccgttc cagtcgatcc cgtagcgctc ggcgtcgtcg
      301 gggtgcgcga gcacgagccc gaccgtgtcg cggatctcct gcttgagcgc ctccatcccg
      361 ccgacgtcct cgaaggtcgt gagctcctcg ggcggctgca cgtcgaagtt gccgctcccg
      421 ccgccgagcc cgcccatcat cccgccgccg agcagcccgc cgagcgggct cgactgctgc
      481 gccgggcgcc tgcgcgtccg ccgcgggcgc cgggggcgcc cggggcgctc gtcgcgctcg
      541 cgctccgtgc gctcgggcac agcctccgcc tcgggctcgg gctcggggga gacgtcggtg
      601 atctcgaccc actggccggt ctcgaagtcg tacgtgtacg actgcgcctc gccgaagaac
      661 aggttgaagg ggaacggcag ctccggctga ggcgactcgc cgtttccggc gtgccggcgc
      721 agctcctcgc ggcgccgagc ctcctcctcg agcgcgttcc ggcgcatgcg gcgcgcgctc
      781 atggacatga gcgccatcac gaccaccatc acgacgaacg ccaggagcag caggctcacg
      841 cgacgcgaag ttccccccgg gtcgggccgc taaccccttt gccggagcgg tgggcggagc
      901 tcagccggcg cggcgccgcg ccatgagcca gatcccggcg gcgaaggcgg cggcgatccc
      961 gagcgcgagc gcgacgagca gcggggcgga gacgccgtcg tcgtcctcgt ccgaggcctg
     1021 cacggcggtg gtctcggtgt cctccgtctc ctcctcctcg gactcggtct cgctctcctc
     1081 ttcggtggtc gtgggtggcg gaggcggcgg ctcctcggcc ggcgcgggcc tgatcgcgag
     1141 cgacggcgcc gacggatcga acggcgtgac ggtgaggagc ggcgccgggt tctcggagcc
     1201 cggctcgccg atccactcca cgacctggtc gtcggagtac gtctgcgtgg cctggaacgc
     1261 cagcgtctgc ccggccgcgc gcggaaccgc gagccggagc gcgaacgact ggctgttcga
     1321 gcccgtgatc tcgccgccgc tccactcgat caccgtgatg cgctcgtcga cccgccggtc
     1381 gcccacgagg agcggctcgt cgagcggctc gcgctccacg ttcacctgcc agccgggcgt
     1441 cggcggcacg cggacgaaga gcaccgagtc cgggacctgc acggccaccc ggacggtgct
     1501 cgcgttcggc tcctcgttcg gaacctgcag ctgcagccgc gcgaagctgc gcgccgggac
     1561 ctcacccggc gcaaaggcga agtgcgcgtg cgcggccggg gcggccacaa gggcgccgac
     1621 ggcggcgacg agcgcgacgc gcctgcggct catcggccga tcttaggcat gcgccgcccg
     1681 ttcaggcgcc ggtcaggcgg aactcggccc agagcgggta gtggtcggag atccgcgcct
     1741 ccttccgccg gcgcggcacg tcctggagga tgtgatcggt ccagaggaat ccgcccgcac
     1801 ggccgctgta ctcgagcgtg agcgcggagc ggtcgccggt cgtgaaccac gcgatctggt
     1861 cgaagtacga ggtgtcgccc tccgtgtcgg ccagggttcg cggaacgtcg aagagcgcgg
     1921 ccggcggcga gaggccgtac tcgacgacga acgcgcgcca gttcgggtcg tcgtagcggt
     1981 cgatgttgaa gtcgccgagc gcgatgaggt tccggttgaa gtcctccggg tcggtcgacg
     2041 cctcggagag ccagcgcgcg atgcgaagga gctcgggcgt gcggtccgcg gccttcttcc
     2101 cccagagcac gtggagcgag acgagggtga acgcgtcgcg cccggcgcgg aagctgaccg
     2161 cgtacggcgt ccgggcgaac tgccggcgca tgatcgcgtc cgcgccctgg agctcctcgt
     2221 ccgggatcac gagctcgccc gccatccccg tgagctgcac gcgccgcgtg tcgaagagga
     2281 acccgagccg ctcgtcgttc ccgggcttcc ccacgaccgt gtcggtcacg aggaacgacc
     2341 agttcggccc gaggagctcc atgagcaggc gaagcgcctc gagcgtgtgc cggatctcga
     2401 cgagcgcgac gacgtcgaag cgcgacacga cctcggcgat gcaccagacg tcctgcaggt
     2461 tccgcttcgg cgagtcgtcc tcgcgcggcg tccaccgctt cgtcacgcgc gagagcgcct
     2521 ggacgttcca cgtgccgatc aggaggttcc ggtcgacgac cttcgccggg atcgccgcgt
     2581 cgagcccggc ggcgagggcg gcgaccgcg
//
LOCUS       DASTFC010000147         6254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_678318,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000147 DASTFC010000000
VERSION     DASTFC010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678318"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFO88_04405"
     CDS             <1..298
                     /locus_tag="VFO88_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544796.1"
                     /translation="IAAEPVSDDELQKARNFAKGRLVLSLEDPRGTILFGLRSEVLEG
                     KIREPDEVLAGLDAVTVEDVQRVAQELIAEDRLNLALIGPFEDESRFEPLLAGA"
     gene            complement(295..633)
                     /locus_tag="VFO88_04410"
     CDS             complement(295..633)
                     /locus_tag="VFO88_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544797.1"
                     /translation="MTRIALVVALLVFALAGCGGADEPAGPPERPAEYEGILEESDGE
                     GRLLVRAEGDACGIWTSPEEGARVFDSTGAEIGWAELEPGARVALWIPGPIAESCPMQ
                     GAAEAVAVLP"
     gene            complement(669..1772)
                     /locus_tag="VFO88_04415"
     CDS             complement(669..1772)
                     /locus_tag="VFO88_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9544798.1"
                     /translation="MADVLMYGDPLRSPEIRHEVPLGSPDPVVYVERNGAREVFLGSL
                     DVPLLASADGLVARPFEELGNDELVAAGLPQRERETELVLRACRLAGIERARVPAGFP
                     LAVADHLRAHGVELEPDGELFDTRRRAKSETELAGMRRAVRASELALTRVRELLGSGE
                     RQTSEGLRTEVLRVFTEEGVASPDPPIVSHGPQTAIGHEPGHGEILAGEPVVCDLYPL
                     DPESGCYSDMTRTFCVGEPPAELAEYWELCRTALERVLAEVRPGVSGRELYRIAAETF
                     ADAGQPTQLTKEPGVPLDRGFFHSLGHGVGLEVHEAPSLGRVGEDLVAGDVIAIEPGC
                     YRPGFGGCRLEDLVLVTEDGAERLTTFPYGLVV"
     gene            1848..3392
                     /locus_tag="VFO88_04420"
     CDS             1848..3392
                     /locus_tag="VFO88_04420"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6640 family putative metalloprotease"
                     /protein_id="HET9544799.1"
                     /translation="MPARLLLALLAALACAGALAAPAAAERDALDRALASGRISQAAY
                     ALERARSLTDLGAVRRRRPGAARPDGHDVTGILRDLRAAAPLLHGDERREAERMLARP
                     SSGAGDAGLHGWPTGAAMESLCAELCLHWIETGGAAPPPEDENADGVPDWVERSFAAL
                     GTALARFDGLGYRRPRSDLASAEPELDGRLDVYLGDLGRFGYFGYCTTDDDAAWYARR
                     VSAYCVLDNDFLDPELRLGSPLTGLRATAAHELFHAVQVSYDWLEDLWFMEGTATWME
                     DVAFPGVHDSLRYLRRSPIGRPHVPLDGYLYGTWIFWRYTAERLGPRVVRDTWERAGR
                     DTYSLAAALRAVEARDVSFPRLFSGFAAANRVPRTVYEEGHLYGRARAFAAYGLGDAR
                     PSVEGSTAIAHLASKTVALLPVSGLGPRSLALDVTVGSGAASSRATALVVRPGGDVER
                     RRIALDAAGTGRVVVPFGAGVRRVDLVLVNAGTRYRCRRGTELSCGGVSLDDGRPFAF
                     RAALLR"
     gene            3404..4558
                     /locus_tag="VFO88_04425"
     CDS             3404..4558
                     /locus_tag="VFO88_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9544800.1"
                     /translation="MEKEVFERARALVARYDEELRRALPDGIQVFDAHVHVGSDIDGF
                     VAPLEDLLAVQEHYGIARAFAFCMDEPDRHPAFRAANDRTLAAAERSDGRLIPFVRLD
                     LAVEPVEEATRCLDLGARGIKLHPRAQGFLLNDDRLAPVFALAAERRVPILIHGGRGL
                     PPIAEELGRLVEAHPEAQLIIAHGGIADLAALSERLAGRPGVYFDTSVWSPVDLLDVF
                     HRFSPEQALYASDYPYGQQPTSLLLAIKVARAAGLGDDALRAMLWETATRIAAGEPPV
                     EASAPSPRETITQPIAFARIHNYLTMATPLLWTRQPDTIGVLGLALNACAERDGFAEA
                     RERIAELVSCARDLWRTAAEAADESERREIGRATFRLIHLADVETVTAGL"
     gene            4617..5354
                     /locus_tag="VFO88_04430"
     CDS             4617..5354
                     /locus_tag="VFO88_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HET9544801.1"
                     /translation="MRGSAAETPRLTASHLEVLAPPAAPAPETSPLGAARVRRRLTVE
                     EAAVRAGLAPEEIRSLEESRIYRFPSVNDAIAATIVYAQALGITGREARGLAGLDGGR
                     SGFRWRRLAAVVAFLAVAGVLGWFVAVPQLRDAVSPAPSAAAQAERQLPPPWEIRVDV
                     FNGTELPNAAAVMANEIAGPLAYRLGTVENAERLDYVQTRVYYPPGSHDIAKRLADDL
                     AVQTTALPTGDDANRLVVIVGLDRASG"
     gene            complement(5351..5578)
                     /locus_tag="VFO88_04435"
     CDS             complement(5351..5578)
                     /locus_tag="VFO88_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9544802.1"
                     /translation="MTDPCDKCEELLQPFLDRELTDAEREEAETHLDACTYCRKRYKF
                     EESLRRYVVSACREPMPADLKAKLAALRTPL"
     gene            complement(5575..6162)
                     /locus_tag="VFO88_04440"
     CDS             complement(5575..6162)
                     /locus_tag="VFO88_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9544803.1"
                     /translation="MSETQPEVDARRMAEEARDRVRFEEEVLELADQAYRVARRLAPT
                     QEEAEDLVQETFARAFRSWRQYQPGTNLRAWLFRILTNLNIDRGRKVQRTPDIQPMEE
                     GDYYLYNRLEESGAGEDEDRVVERLSQDSIVEALAAVPHDFRDVVVLVDIGDFSYADA
                     AQILDIPIGTVMSRLHRGRRNLKQALAEAAVTEAS"
BASE COUNT          783 a         2387 c         2266 g          818 t
ORIGIN      
        1 gatcgcggcg gagccggtgt cggacgacga gctccagaag gcacggaact tcgccaaggg
       61 ccggctcgtg ctctcgctcg aggacccgcg cgggacgatc ctcttcggcc tgcggagcga
      121 ggtgctcgag gggaagatcc gcgagccgga cgaggtgctc gccggcctcg acgcggtgac
      181 ggtcgaggac gtccagcgcg tcgcgcagga gctcatcgcg gaggaccggc tgaacctcgc
      241 gctcatcggc ccgttcgagg acgagagccg cttcgagccg ctgctcgcgg gcgcctaggg
      301 caggacggcg accgcctcgg cggcgccctg catggggcac gactcggcga tcgggccggg
      361 gatccagagc gcgacgcggg ctcccggctc gagctcggcc cagccgatct cggcgccggt
      421 cgagtcgaag acgcgcgcgc cctcctccgg ggaggtccag atcccgcagg cgtcgccctc
      481 ggcgcggacg agcaggcggc cctcgccgtc gctctcctcg aggatcccct cgtactccgc
      541 cgggcgctcc ggcgggccgg cgggctcgtc cgcgccgccg cagcccgcga gcgcgaagac
      601 gagcagggcg acgacgagcg cgatccgggt catcgtccct tcagacgccc gcccgggccc
      661 ggaagttcct agacgacgag gccgtacggg aaggtcgtca gccgctcggc gccgtcctcc
      721 gtcacgagaa cgagatcctc gaggcggcag ccgccgaacc cggggcggta gcagccgggc
      781 tcgatcgcga tgacgtcgcc ggcgacgaga tcctcgccga cgcgcccgag cgaaggcgcc
      841 tcgtgcacct cgagcccgac gccgtgcccg agcgagtgga agaagccgcg gtcgagcggg
      901 acgcccggct ccttcgtgag ctgggtcggc tggccggcgt cggcgaacgt ctcggcggcg
      961 atccggtaga gctcgcgccc gctcaccccc gggcgcacct ccgcgaggac gcgctcgagc
     1021 gcagtgcggc agagctccca gtactcggcg agctcggcgg gcggctcgcc gacgcagaac
     1081 gtccgcgtca tgtcggagta gcagccggac tccgggtcga gcggatagag gtcgcagacg
     1141 accggctcgc ccgcgaggat ctcgccgtga cccggctcgt gcccgatcgc ggtctggggg
     1201 ccgtgcgaga cgatcggcgg atcgggcgac gcgaccccct cctcggtgaa gacgcgcagc
     1261 acctcggtcc gaagcccctc gctcgtctgc cgctcgccgc ttccgagcag ctcgcggacg
     1321 cgcgtgaggg cgagctccga cgcccggacc gcgcggcgca tcccggcgag ctccgtctcg
     1381 ctcttcgcgc gccggcgggt gtcgaagagc tccccgtcgg gctccagctc gaccccgtgc
     1441 gcgcgcaggt ggtccgcgac cgcgaggggg aagccggccg gcacgcgcgc gcgctcgatc
     1501 ccggcgagcc ggcaggcgcg caggacgagc tccgtctccc gctcgcgctg cgggagcccg
     1561 gcggctacga gctcgtcgtt tccgagctcc tcgaacggcc gcgcgacgag cccgtccgca
     1621 gacgcgagca gcggaacgtc gagcgagccg aggaagacct cgcgcgcgcc gttccgctcg
     1681 acgtagacga ccgggtccgg cgagccgagc gggacctcgt gacggatctc cggcgagcgg
     1741 agcgggtcgc cgtacatgag gacgtcggcc acccgcgcga tggtagctgc acgccgccac
     1801 cccgccgggg gatgcgcgtc cgcgggcggc gcgccgatcc accccggatg ccggcgcgcc
     1861 tcctcctcgc cctgctcgcg gcgctcgcct gcgcgggcgc cctcgccgcg ccggcggcgg
     1921 ccgagcggga cgccctcgac cgtgcgctcg cgtccggccg gatctcgcag gccgcgtacg
     1981 cgctcgagcg ggcgcgctcg ctcaccgacc tcggcgccgt gcgccggcgc cggccgggcg
     2041 cggcccggcc cgacgggcac gacgtgaccg gcatcctccg ggatctccgc gccgccgccc
     2101 cgctcctgca cggcgacgag cggcgcgagg cggagcggat gctcgcccgc ccctcctcgg
     2161 gcgccggcga cgccggcctc cacggctggc cgaccggcgc ggcgatggag agcctctgcg
     2221 ccgagctctg cctccactgg atcgagacgg gcggcgcggc tccgccgccc gaggacgaga
     2281 acgcggacgg cgtccccgac tgggtcgagc ggtcgttcgc ggccctcggg accgcgctcg
     2341 cgcgcttcga cgggctcggc taccgccggc cgcgcagcga cctcgcctcg gccgagccgg
     2401 agctcgacgg gcggctggac gtctacctgg gcgacctcgg ccgcttcggg tacttcggct
     2461 actgcacgac ggacgacgac gccgcgtggt acgcgcgccg cgtgtcggcg tactgcgtcc
     2521 tcgacaacga cttcctcgac cccgagctcc gcctcggctc gccgctgaca gggctccgcg
     2581 cgaccgccgc gcacgagctc ttccacgccg tccaggtcag ctacgactgg ctcgaggacc
     2641 tctggttcat ggagggcacc gcgacgtgga tggaggacgt cgcgttcccc ggcgtccacg
     2701 acagccttcg ctacctccgg cgcagcccga tcggacggcc gcacgtgccg ctcgacggct
     2761 acctctacgg cacgtggatc ttctggcgct acacggccga gcggctcggc ccgcgggtcg
     2821 tccgcgacac gtgggagcgc gccgggcgcg acacgtactc gctcgcggcg gcgctgcgtg
     2881 cggtcgaggc gcgcgacgtc tcgttcccgc gcctcttctc cggcttcgcg gcggcgaacc
     2941 gcgtgccgcg gacggtgtac gaggagggac atctctacgg gcgggcgcgg gcgttcgcgg
     3001 cctacgggct cggggacgcg cggccgagcg tcgagggctc gacggcgatc gcgcacctcg
     3061 cgagcaagac ggtcgcgctc ctgcccgtct ccggcctcgg gccgcgctcg ctcgcgctcg
     3121 acgtgacggt cggaagcgga gcggcgagct cgcgcgcgac ggcgctcgtc gtccggcccg
     3181 gcggcgacgt cgagcggcgg cggatcgccc tcgacgcggc cgggaccggc cgcgtcgtcg
     3241 tccccttcgg cgcaggcgtg cgccgcgtcg acctcgtcct cgtgaacgcc gggacgcgct
     3301 atcgctgccg gcgcggcacc gagctctcct gcgggggagt ctccctggac gacggtcgcc
     3361 cgttcgcgtt tcgcgcggcg ctgctgcgat agcctcacgc cccgtggaga aggaggtctt
     3421 cgagcgggcc cgcgcgctcg tcgcgcgcta cgacgaggag ctgcgccgcg cgctccccga
     3481 cgggatccag gtcttcgacg cccacgtcca cgtcggctcg gacatcgacg ggttcgtcgc
     3541 gccgctcgag gacctgctcg cggtgcagga gcactacggg atcgcccgcg cgttcgcgtt
     3601 ctgcatggac gagcccgacc ggcacccggc cttccgcgcg gcgaacgacc ggacgctcgc
     3661 ggccgcggag cggagcgacg ggcggctcat cccgttcgtg cggctcgacc tcgccgtcga
     3721 gccggtcgag gaggcgacgc gctgtctcga cctcggcgcc cgcggcatca agctccaccc
     3781 gcgcgcccag ggcttcctcc tgaacgacga ccgcctcgcg cccgtgttcg cgctcgcggc
     3841 cgagcggcgc gtgccgatcc tcattcacgg cgggcgtggg ctgccgccga tcgcggagga
     3901 gctcggacgg ctcgtcgagg cgcacccgga ggcgcagctc atcatcgcgc acggcgggat
     3961 cgccgacctc gccgcgctct cggagcggct cgcgggccgg ccgggggtgt acttcgacac
     4021 ctcggtctgg agcccggtcg acctgctcga cgtgttccac cgcttctcgc ccgagcaggc
     4081 gctctacgcc tccgactacc cgtacgggca gcagccgacc tcgctcctcc tcgcgatcaa
     4141 ggtcgcgcgg gccgccgggc tcggcgacga cgcgctccgc gcgatgctct gggagaccgc
     4201 gacacggatc gcggccggcg agccgcccgt cgaggcgtcc gcgccgagcc cccgcgagac
     4261 gatcacccag ccgatcgcgt tcgcgcggat ccacaactac ctcacgatgg cgaccccgct
     4321 cctctggacg cggcagccgg acacgatcgg ggtcctcggc ctcgccctga acgcctgcgc
     4381 ggagagggac gggttcgccg aggcgcgcga gcggatcgcc gagctcgtct cgtgcgcgcg
     4441 cgacctttgg cgcacggccg ccgaggccgc ggacgagtcc gagcgcaggg agatcggccg
     4501 ggcgacgttc cggctcatcc acctcgcgga cgtcgagacg gtcacggccg ggctgtaaga
     4561 gctcgttccg aagcgagctc tctaaactcc cgcgccgccc tgccgatgga ggcagagtga
     4621 ggggaagcgc cgcagaaacg ccacgcctca cggcgtcgca cctcgaggtc ctcgctcctc
     4681 cggccgcgcc ggcgcccgag acctccccgc tcggcgccgc ccgcgtccga cggcggctga
     4741 ccgtcgagga ggcggccgtc cgcgccggtc tcgccccgga ggagatccgc tcgctcgagg
     4801 agagccggat ctaccggttc ccctccgtga acgacgcgat cgccgcgacg atcgtctacg
     4861 cgcaggcgct cgggatcacc ggccgcgagg cgcgcgggct cgccgggctc gacggcggcc
     4921 gcagcggctt ccgctggcgc cggctggccg cggtcgtcgc gttcctcgcc gtcgccgggg
     4981 tgctcggctg gttcgtcgcc gtgccgcagc tccgcgacgc cgtgtcgccc gcgccgtcgg
     5041 ccgccgcgca ggccgagcgg cagctcccgc ccccctggga gatccgcgtc gacgtcttca
     5101 acggcaccga gctgccgaac gccgcggcgg tcatggcgaa cgagatcgcc ggccccctcg
     5161 cgtaccgcct cgggacggtc gagaacgccg agcggctcga ctacgtccag acgcgcgtct
     5221 actacccgcc tgggagccac gacatcgcga agcgcctcgc cgacgacctc gcggtccaga
     5281 cgaccgcgct cccgacgggc gacgacgcga accgcctcgt cgtgatcgtc gggctcgacc
     5341 gagcctcggg ctagagcggc gtccggagcg cggccagctt ggccttgagg tccgccggca
     5401 tcggctccct gcaggccgag acgacgtagc ggcgcagcga ctcctcgaac ttgtagcgct
     5461 tccggcagta cgtgcacgcg tcgaggtgcg tctccgcctc ctcccgctcc gcgtcggtga
     5521 gctcgcggtc gaggaagggc tggagcagct cctcgcactt gtcgcaggga tccgtcacga
     5581 ggcctcggtc acggctgctt cggccaatgc ctgtttcagg ttacgtcgcc cacgatgaag
     5641 acgcgacatg acggtgccga tcgggatgtc gaggatctgc gccgcgtcgg cgtaggagaa
     5701 atccccgatg tcgacgagga cgacgacgtc gcggaagtcg tgcgggaccg cggcgagcgc
     5761 ctcgacgatc gagtcctggg agagccgctc gacgacccgg tcctcgtcct cccccgcgcc
     5821 cgactcctcg agccggttgt agaggtagta gtcgccctcc tccatcggct ggatgtccgg
     5881 cgtgcgctgc accttgcggc cgcggtcgat gttgaggttc gtgaggatgc ggaagagcca
     5941 ggcccgcagg ttcgtgcccg gctggtactg ccgccaggag cggaaggcgc gcgcgaaggt
     6001 ctcctgcacg aggtcttccg cctcctcctg ggtcggcgcc agccgccggg ccacccggta
     6061 cgcctgatcc gccagctcga gcacctcctc ctcgaagcgc acgcggtcgc gcgcctcttc
     6121 cgccattcgc ctggcgtcga cctccggttg cgtctcgctc accgcggccg acactagcaa
     6181 tcaacgatgg gcggccatgg ctattccgcg gaacgccgat tgtggtgaca atcgagacaa
     6241 gacgcgctat ggtc
//
LOCUS       DASTFC010000148         7865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_101736,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000148 DASTFC010000000
VERSION     DASTFC010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101736"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFO88_04445"
     CDS             complement(<1..245)
                     /locus_tag="VFO88_04445"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9544804.1"
                     /translation="MSASPSNRALRVRLSALVFTAGAATLATEIAASRLLAPYFGSST
                     IVWANIIGLTLLYLSLGYWLGGKVADRRPEPRVLGGIV"
     gene            complement(253..2997)
                     /locus_tag="VFO88_04450"
     CDS             complement(253..2997)
                     /locus_tag="VFO88_04450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9544805.1"
                     /translation="MAAPSVPAHPLDRAPDDAEARLFDGLAAGARALVAGAAPADALA
                     VIARGVAESLAAPLVVVRAAEAGSGELVAVALHAASDALAAEVEGSRLPAGSIGTADA
                     DLDAADRATPAALRRTAARARASRALVVPVAEGERPAATLEVYRAGPPFGELERALAR
                     LAAAHVELALASGASLGGADLPSRVRLALELAGEALAAGSDEAELAEHVVRLAVDATG
                     AEGAVLWRVAQGSPPVPLARHGEAETGGAAAEQVRGALEARDGNGDAGRSTPLVVPLG
                     EPPAGALTLVFAGEPAPDALDALGGLAARAGVALRRTRRAQEIAGALERSQTVVAVVS
                     QAIAQLSLAHTLETAVERVGELTASEDVGLYLREGERLVAASVRGRAGDTALAERLLE
                     LALGPFRSRGFLLLPDLASDLRLAGLEGVLAETGLRRALVVPLVVQDEVIGALAVFDR
                     APRDHEPGEEGLVIALSSQLAVAVQNARLHERTKELGAVLERTLASERKAARQLRGLF
                     EISHSFTRSLSLDATLDAVAETMVKLFGVDLAAIRLRDARRGELVLHALRVADRSLEE
                     PAAAMLARPMPLDDPLVARVLAERRPLLLAAGRSDVPPDSILQPFLGKGSTAAMLPLA
                     TPGEVLGTLTLVSLDPGRPLDEETLETAATVTAQAALAIDNARLSQQRRDFTETMQRS
                     LLPRTLPEVPGLEIGHVYQSASRVDVGGDVYDLLALDDGRIAVCLGDVVGKGIEAAAD
                     MTMAKFAFRSLARLYPEPGTFLAHTNDVVVEEIAPGKFLTLLYVVADPRTGELACANA
                     GHPPLRLVQPDGTVQALPAPGLALGIDPGQEYPEERVVAAPGSSAVLYTDGVIECRSN
                     GELYGEERLDRFLAANAGLGAQELADALLADCRAFAGGELDDDCAIVVLRVAPGVSVG
                     "
     gene            3074..3313
                     /locus_tag="VFO88_04455"
     CDS             3074..3313
                     /locus_tag="VFO88_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544806.1"
                     /translation="MAAPAPRRARVERVVTHQEPTRLQRSLVRERAKRRARIEHERER
                     RRARRRFAVLMLALLFFAAVLSLTIWDEIETLFGL"
     gene            3340..3750
                     /locus_tag="VFO88_04460"
     CDS             3340..3750
                     /locus_tag="VFO88_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544807.1"
                     /translation="MRQAAHWTPGEAVAWMAAIVVTISPFLGWYSGTLDGLPLSVVGW
                     DTGAIGKLVLLVGLAALVVLGMRAAGIELPPTVPFGMIVAALGAIATVFILIRVATIP
                     DDYVELGRAVGLWISLVAAVLLIVGGLLKSAEDA"
     gene            complement(3686..4123)
                     /locus_tag="VFO88_04465"
     CDS             complement(3686..4123)
                     /locus_tag="VFO88_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9544808.1"
                     /translation="MIAARRGLPLLRTTALVAATVWLTDLITLGIKLSVDRPRPFEVL
                     AEADPLIVASVGTSFPSGHASMSAAGAIVLASLAPRAWPLLALLAVVVAVSRVYVGVH
                     YPLDVLTGLALGTIVGTAVVLALRRLRRTSGGPLRSAAPPPRG"
     gene            complement(4252..4845)
                     /locus_tag="VFO88_04470"
     CDS             complement(4252..4845)
                     /locus_tag="VFO88_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9544809.1"
                     /translation="MPGEERSGPAPGGGVAPSDWLTLGQAARYLGVAQSTIRKWSDQG
                     RLPAFYTPGGHRRFRRGDLDRFLGSSRANTVASSRVLIVDDDGGLRTFLRTALEGDGC
                     VVREAASAEEGLAALDEELPDLILLDVEMPGMNGLEMLQRVRERHGVETIPVVLFSGK
                     VEAEDEAEEAGAQAFIGKPFDPAQLLASTRQLLRARP"
     gene            complement(4927..5145)
                     /locus_tag="VFO88_04475"
     CDS             complement(4927..5145)
                     /locus_tag="VFO88_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544810.1"
                     /translation="MTELGPSGRRERARRERRRTRRRRAGLVLGGLALAVVVFFAGLA
                     LGRALEEGPEPAAPQTLVRTLEPGTLPR"
     gene            complement(5142..5456)
                     /locus_tag="VFO88_04480"
     CDS             complement(5142..5456)
                     /locus_tag="VFO88_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544811.1"
                     /translation="MTLDVARHQWADGLRRLGTERGNRARQLADLVDAVQDELRRRIG
                     QTFTLAELADAYAGSEDWVRDIVVESAPPPPRAEAGLRDAALVGDAAFASYARGATDY
                     RP"
     gene            complement(5453..5920)
                     /locus_tag="VFO88_04485"
     CDS             complement(5453..5920)
                     /locus_tag="VFO88_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HET9544812.1"
                     /translation="MDDLGVVSWQLGRPARPFRRVAVRCAYGFPAVTEQEPFTSSGTP
                     FPTTYYLTCPWLVAGIARLEAAGGVQRWTDAAARDPELAASLARADAEQRALRPELDV
                     GIAGTREVGPLKCLHAHAAFALARPGYELGDRILDEAGETWCPDGRCAEALPA"
     gene            complement(5967..6260)
                     /locus_tag="VFO88_04490"
     CDS             complement(5967..6260)
                     /locus_tag="VFO88_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET9544813.1"
                     /translation="MARKRRRPSRSSIALRWLAVVALVAIAAAYVHPIRSYMDARGQV
                     ERRKADIAALEREQERLERRIDVAETDEFVEREARKLGLVRPGEKLFIVNGVG"
     gene            complement(6383..>7865)
                     /gene="pyk"
                     /locus_tag="VFO88_04495"
     CDS             complement(6383..>7865)
                     /gene="pyk"
                     /locus_tag="VFO88_04495"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9544814.1"
                     /translation="RSPLGYRGRVRRDRSIVAAGKARTTRRTKIVCTIGPSTSSPELV
                     ERLVEAGMDGARLNFSHGTHEEHAERAELVRAAQEKARRPLAIIADLQGPKLRIGKLA
                     QPRVLAEGDHVVVVGSGDGSDDGHLPVTPPVLPEVLRRGDDILIDDGLVHLRVEAVEA
                     GRVEAVVLEGGAVTANKGVNVPGVPLPIPSVTEKDTADLEFALGLKVDFVALSFVRSA
                     NDVRQLRELLHAAGSNAQIIAKIEKAEAVDDLTGILRLADAVMVARGDLGVEIGPAEV
                     PLVQKRILLAALERGKPAITATQMLESMISQSEPTRAEASDVANAILDGTSAVMLSGE
                     TAVGRYPIESVETMVRIACAVEPSLAYRHELHTSPDEPYSTVGEAMSNAACDIAEALD
                     AKAILVPTYSGRTASAVARHRPRRPVIAVTHRHHAAQQLALEWGIVPAIIPECDDVED
                     LWARSLEAARSTGLVASGDRVVITAGTAVNVPGTTNLIKVETA"
BASE COUNT          995 a         2937 c         2989 g          944 t
ORIGIN      
        1 acgatcccgc cgagcacgcg cggctccggg cgccggtcgg ccaccttccc gcccagccag
       61 tagccgagcg agaggtagag gagcgtgagc ccgatgatgt tcgcccagac gatcgtcgac
      121 gagccgaagt agggcgcgag gaggcgcgac gcggcgatct ccgtggcgag cgtggcggcc
      181 ccggcggtga agacgagcgc ggacaggcgg acgcgcagcg cccggttcga gggggaggcc
      241 gacatccggc ttctagccta cggacacgcc gggcgcgacc cggaggacaa cgatcgcgca
      301 gtcgtcgtcc agctcgccgc ccgcgaacgc gcggcagtcg gcgaggagcg cgtccgcgag
      361 ctcctgcgcg ccgagaccgg cgttcgcggc gaggaagcgg tcgaggcgct cctcgccgta
      421 gagctcgccg ttcgagcggc actcgatcac gccgtccgtg tagaggacgg ccgaggagcc
      481 gggcgccgcg acgacgcgct cctcggggta ctcctgcccg ggatcgatcc cgagcgcgag
      541 ccccggcgcc ggcagagcct gcaccgtccc gtcgggctgg acgagccgga gcggcggatg
      601 ccccgcgttc gcgcaggcga gctcgccggt gcgcgggtcg gcgacgacgt agaggagcgt
      661 gaggaacttc cccggggcga tctcctcgac cacgacgtcg ttcgtgtgcg cgaggaacgt
      721 gcccggctcc gggtagaggc gcgcgagcga gcggaaggcg aacttcgcca tcgtcatgtc
      781 ggccgccgcc tcgatcccct tgccgaccac gtcgccgagg cagaccgcga tccggccgtc
      841 gtcgagcgcg agcaggtcgt agacgtcgcc gccgacgtcc acgcgcgagg ccgactggta
      901 cacgtgcccg atctcgagcc ccgggacctc gggcagcgtg cgcggaagga gcgagcgttg
      961 catcgtctcc gtgaagtcgc gccgctgctg cgagaggcga gcgttgtcga tcgcgagcgc
     1021 ggcctgggcg gtgacggtcg cagcggtctc gagcgtctcc tcgtcgagcg gccggccggg
     1081 gtcgagcgag acgagcgtga gcgtgccgag cacctccccg ggcgtcgcga gcggcagcat
     1141 ggctgcggtc gagcccttgc cgaggaacgg ctggaggatc gagtcgggcg gcacgtcgct
     1201 gcgccccgcg gcgagcagga gcggccgccg ctccgcgagg acgcgggcga cgagcgggtc
     1261 gtcgagcggc atcggccggg cgagcatcgc cgccgccggc tcctcgaggc tccggtcggc
     1321 gacgcggagc gcgtgcagga cgagctcgcc gcggcgcgcg tcgcgcagcc ggatcgcggc
     1381 gaggtcgacg ccgaagagct tcaccatcgt ctcggcgacc gcgtcgagcg tcgcgtcgag
     1441 cgagaggctg cgcgtgaacg agtgcgagat ctcgaagagc ccgcggagct ggcgcgccgc
     1501 cttccgctcc gacgcgagcg tgcgctcgag gacggcgccg agctccttcg tccgctcgtg
     1561 gaggcgcgcg ttctggacgg cgaccgcgag ctggctcgag agcgcgatca cgaggccctc
     1621 ctccccgggc tcgtggtcgc gcggggcgcg gtcgaacacg gccagcgcgc cgatcacctc
     1681 gtcctgcacg acgaggggga cgacgagcgc gcggcgcagg ccggtctcgg cgaggacgcc
     1741 ttcgagcccg gcgagccgca gatcgctcgc gaggtcgggc aggaggagga agccgcggct
     1801 cctgaacggg ccgagcgcga gctcgagcag gcgctcggcg agcgcggtgt cccccgcgcg
     1861 gccgcgcacg ctcgcggcga cgaggcgctc gccctcgcgg agatagaggc cgacgtcctc
     1921 gctcgcggtc agctcgccga cgcgctcgac ggcggtctcc agcgtgtgcg cgagcgagag
     1981 ctgcgcgatc gcctggctca cgacggcgac gacggtctgc gagcgctcga gggcgccagc
     2041 gatctcctgg gcccggcgcg tgcggcggag cgcgacgccc gcccgcgccg cgaggccgcc
     2101 gagcgcgtcg agcgcgtcgg gcgccggctc gcccgcgaac acgagcgtga gcgcgcccgc
     2161 cggcggctcg ccgagtggga cgacgagcgg cgtgcttcgg cccgcgtcgc cgttgccgtc
     2221 gcgcgcctcg agcgcgccgc gaacctgctc ggcggcggct ccgcccgtct ccgcctcgcc
     2281 gtggcgcgcg agcgggacgg gcggcgagcc ctgggcgacg cgccagagga cggcgccctc
     2341 cgccccggtc gcgtcgaccg cgagccggac gacgtgctcg gcgagctccg cctcgtccga
     2401 gccggccgcg agcgcctcgc cggccagctc gagggcgagc cggacgcggg acggcaggtc
     2461 ggcgccgccg agcgacgcgc cgctcgcgag cgcgagctcc acgtgcgcgg ccgcgagacg
     2521 ggcgagcgcg cgctcgagct cgccgaacgg cgggcccgcg cggtagacct cgagcgtcgc
     2581 ggcgggccgc tcgccctccg cgaccggcac gacgagcgcc cgtgaggccc gcgcgcgggc
     2641 ggcggtccgc cggagcgccg cgggcgtcgc acggtcggcc gcgtcgaggt cggcgtccgc
     2701 cgtgccgatg gagcccgccg gaaggcgcga gccctcgacc tccgcggcca gcgcgtccga
     2761 tgcggcgtgg agggcgaccg cgacgagctc gccgcttccc gcctcggccg cgcggacgac
     2821 gacgagcggg gcggcgagcg actccgccac gccgcgggcg atcacggcaa gcgcgtcggc
     2881 gggcgcggcg cccgcgacga gcgcgcgcgc accggctgcg aggccgtcga agagccgcgc
     2941 ctccgcgtcg tcgggcgcac ggtcgagcgg gtgcgcgggt acgctcggcg cggccatgtc
     3001 acgaatgatg ccttcgccgc gggcaggggg ttcggcttgc gccgtccgcc cccagcggga
     3061 acaatcgcct tccatggcgg ctccggcacc ccggcgcgcc cgcgtggagc gggtcgtgac
     3121 ccaccaggaa ccgacccggc tgcagcgctc gctcgtcagg gagcgcgcca agcggcgcgc
     3181 ccgcatcgag cacgagcgcg agcgtcgtcg ggcacggcgg cgcttcgccg tcctcatgct
     3241 cgccctgctc ttcttcgcgg cggtcctctc gctcacgatc tgggacgaga tcgagacgct
     3301 cttcggccta tgacggtcgc gcccccgcga ccggcctcag tgcgccaggc agcccactgg
     3361 acgcccggcg aggccgtggc ctggatggcg gcgatcgtcg tcacgatctc gcccttcctc
     3421 ggctggtact cgggcaccct cgacggcctt cccctctcgg tcgtcggctg ggacaccgga
     3481 gcgatcggga agctcgtgct cctggtcggc ctcgccgcgc tcgtcgtgct cgggatgcgg
     3541 gccgccggga tcgagctgcc gccgaccgtc ccgttcggga tgatcgtcgc ggcgctcggc
     3601 gcgatcgcga ccgtcttcat cctcatccgc gtcgcgacga tccccgacga ctacgtcgag
     3661 ctcggccgcg cggtcggcct ctggatcagc ctcgtggcgg cggtgctgct gatcgtaggg
     3721 ggcctcctga agtccgccga agacgcctga gcgcgagcac gacggccgtc ccgacgatcg
     3781 tcccgagcgc gaggccggtg agcacgtcga gcgggtagtg cacgccgacg tacacgcgag
     3841 agacggcgac cacgacggcg agcagcgcga ggagcggcca ggcccgcggc gcgagcgagg
     3901 cgagcacgat cgccccggcc gcgctcatcg acgcgtggcc cgacgggaac gacgtcccga
     3961 cggaggcgac gatcagcggg tcggcctccg cgagcacctc gaagggccgc ggccggtcga
     4021 cggacagctt gatcccgagg gtgatgaggt cggtgagcca gaccgtcgcg gcgacgagcg
     4081 cggtcgtcct gaggagcggg agcccgcgcc gcgccgcgat caccgcggcg agcgcgatcc
     4141 agacgaggcc cgcgtacccg acgatcgtcc ccgcgaggaa gaccgggtcg agccagccga
     4201 cgcgctggtc gacgatccag aggaagaggt cgcggtcgag gctcgcgagc gtcagggccg
     4261 ggcgcgcagg agctgccgcg tcgaggcgag cagctgcgcg gggtcgaacg gcttgccgat
     4321 gaacgcctgc gcgccggcct cctccgcctc gtcctcggcc tcgaccttcc cgctgaagag
     4381 cacgacgggg atcgtctcga cgccgtgccg ctcgcggacg cgctggagca tctcgagccc
     4441 gttcatgccc ggcatctcga cgtccaggag gatcaggtcg ggcagctcct cgtcgagcgc
     4501 cgcgaggccc tcctcggcgc tcgcggcctc gcggacgacg cagccgtcgc cttcgagcgc
     4561 cgtgcgcagg aacgtccgta ggccgccgtc gtcgtccacg atgaggacgc gcgacgaggc
     4621 gacggtgttc gcgcgggacg agccgaggaa gcggtcgagg tcgccgcgcc ggaagcggcg
     4681 atggccgcca ggcgtgtaga aggccggcag gcgcccctgg tcggaccact tccggatcgt
     4741 gctctgcgcg acgcccaggt accgcgcggc ctggcccagg gtcagccagt cggagggcgc
     4801 gacgccgccc cccggagccg gaccggaacg ctcctcccct ggcatgaggg gggaaggatg
     4861 acacaagccc ggcgctcgtc gcgccgggcc gcggtcggcc gccgtccgcc tcctcggccg
     4921 cccgtgtcac cggggcagcg tccccggctc gagcgtgcgg acgagcgtct gcggggcggc
     4981 cggctcgggc ccttcctcga gcgcgcggcc gagcgcgaga ccggcgaaga agacgacgac
     5041 cgcgagggcg agcccgccga ggacgagacc ggcacgccgg cggcgcgtcc gccgccgctc
     5101 gcggcgcgcg cgctcgcgcc tcccgctcgg cccgagctcc gtcacgggcg gtagtccgtc
     5161 gcgccgcgcg cgtagctcgc gaacgcggcg tccccgacga gggccgcgtc ccgcaggccg
     5221 gcctcggccc gcggcggcgg cggggcgctc tcgacgacga tgtcgcgcac ccagtcctcg
     5281 gagcccgcgt acgcgtccgc gagctcggcg agcgtgaacg tctgcccgat gcggcggcgg
     5341 agctcgtcct gcaccgcgtc cacgaggtcg gcgagctggc gggcgcggtt gccccgctcg
     5401 gttccgaggc ggcggagacc gtccgcccac tggtggcgcg ccacgtcgag cgtcatgccg
     5461 ggagtgcttc cgcgcagcgg ccgtccggac accacgtctc gccggcctcg tccaggatcc
     5521 ggtcgccgag ctcgtagccc ggccgcgcga gcgcgaacgc cgcgtgcgcg tgcaggcact
     5581 tgagcggccc gacctcccgc gtgccggcga tcccgacgtc gagctccggg cgcagcgcgc
     5641 gctgctccgc gtcggcgcgc gcgaggctcg ccgcgagctc cgggtcgcgc gccgcggcat
     5701 cggtccagcg ctggacgccg cccgccgcct cgagccgcgc gatcccggcc acgagccacg
     5761 ggcacgtcag gtagtaggtg gtcgggaatg gggtgccgga gctcgtgaac ggctcctgct
     5821 ccgtgaccgc cggaaagccg tacgcgcagc gaaccgccac gcggcgaaac ggccgcgcag
     5881 gtcgcccgag ctgccaggag accactccga ggtcgtccac cggatcatcg tatccgcgcc
     5941 ccgtgccgtc tccggcgcgg acgccgctac ccgacgccgt tcacgatgaa gagcttctcg
     6001 cccggccgaa cgagcccgag cttgcgcgcc tcccgctcga cgaactcgtc cgtctcggcg
     6061 acgtcgatcc gccgctcgag ccgctcctgc tcccgctcga gcgccgcgat gtccgccttg
     6121 cgccgctcga cctggccgcg cgcgtccatg tagctgcgga tcgggtggac gtacgccgcg
     6181 gcgatcgcga cgagcgcgac gaccgcgagc cagcggaggg cgatggacga gcgggacggg
     6241 cggcgacgct tccgggccat ggaccgtcac cgttcgcagg cggcccgccg cctcctcccc
     6301 gcccggcgcg cccgaagggc ccggggaccc tcgcgctagt aacactttgt tacttggaag
     6361 gggctcggcg ggcgccgacg gcctacgcgg tctcgacctt gatgaggttc gtcgtgccgg
     6421 gcacgttcac ggcggtgccg gcggtgatca cgacccggtc gccggacgcg acgaggccgg
     6481 tcgagcgcgc cgcctcgagc gagcgcgccc agagatcctc gacgtcgtcg cactcgggga
     6541 tgatcgccgg cacgatcccc cactcgagcg cgagctgctg cgccgcgtgg tgacgatgcg
     6601 tgacggcgat gaccggccgg cgcgggcggt gccgcgcgac cgccgaggcc gtgcggccgc
     6661 tgtacgtcgg gacgaggatc gccttcgcgt cgagcgcctc cgcgatgtcg cacgcggcgt
     6721 tcgacatcgc ctcgccgacg gtcgagtacg gctcgtccgg gctcgtgtga agctcgtggc
     6781 ggtacgcgag gctcggctcg acggcgcacg cgatccgcac catcgtctcg acgctctcga
     6841 tcgggtagcg cccgaccgcc gtctcgccgg agagcatgac cgcggaagtc ccgtcgagga
     6901 tcgcgttcgc gacgtcgctc gcctcggcgc gggtcggctc cgactgggag atcatcgact
     6961 cgagcatctg cgtcgccgtg atcgccggct tcccgcgctc gagcgcggcg agcaagatcc
     7021 gcttctggac gagcggaacc tccgccggcc cgatctcgac cccgaggtcg ccccgcgcga
     7081 ccatgaccgc gtcggcgaga cgcaggatcc cggtcaggtc gtcgaccgcc tccgccttct
     7141 cgatcttcgc gatgatctgc gcgttcgagc cggccgcatg gaggagctcg cggagctggc
     7201 gcacgtcgtt cgccgagcgg acgaacgaga gcgcgacgaa gtcgaccttg agcccgagcg
     7261 cgaactcgag gtcggccgtg tccttctccg tcaccgaggg gatcgggagc ggcacgcccg
     7321 ggacgttcac gcccttgttc gcggtgaccg cgccgccctc gagcacgacc gcctcgacgc
     7381 ggccggcctc gaccgcctcg acgcgcaggt gcacgagccc gtcgtcgatc aggatgtcgt
     7441 ccccgcggcg cagcacctcc ggcagcacgg gcggggtgac cgggagatgg ccgtcgtcgc
     7501 tcccgtcccc cgagccgacg acgacgacgt ggtcgccctc cgcgaggacg cgcggctggg
     7561 cgagcttgcc gatccggagc ttcggcccct ggaggtcggc gatgatcgcg agcggccggc
     7621 gcgccttctc ctgcgcggcg cggacgagct cggcgcgctc ggcgtgctcc tcgtgcgtcc
     7681 cgtgcgagaa gttgagccgc gcgccgtcca tcccggcctc gacgagcctc tcgaccagct
     7741 cgggtgagga ggtcgacggc ccgatcgtgc agacgatctt cgtcctgcgc gtcgtgcgcg
     7801 cctttcccgc cgcgacgatt gaccggtcgc gcctcacccg cccacggtag ccgagcgggc
     7861 tccgg
//
LOCUS       DASTFC010000149         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1172694,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000149 DASTFC010000000
VERSION     DASTFC010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172694"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            558..2387
                     /locus_tag="VFO88_04500"
     CDS             558..2387
                     /locus_tag="VFO88_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9544815.1"
                     /translation="MALPLEDYALVGDTQAAALVGRDGSIDWLCVPRFDSPACFAALL
                     GTREHGRWLLAPAGGPPATTRRYAGDSLVLETDFETEDGAVRVTDFMPPRQAAPDLVR
                     IVEGLRGRVAMRMELVVRFDYGSIVPWARRLQEAVSYVGGPDALALETPVETHGENLT
                     TVAEFTVAEGERVPFVLAWHPSHEPPPRSVDAEQALRETLTWWEEWAARCTYEGEWRD
                     AVLRSLTVLKALTYAPTGGIVAAPTTSIPEAIGGVRNWDYRYCWLRDATFTLYALLSS
                     GYTNEARAWRDWLVRAVAGDPAQLQIMYGLGGERRLREHELDWLPGYEGSRPVRIGNA
                     ASAQLQTDVYGEVMDALHNARRVEIEPSDTAWALQRVLLEELEREWTLPDEGIWEVRG
                     PRRHFTHSKVMAWVGFDRAVKAVERFGRDGDAERWKRLRAEIHDEVCREGYDAGRGAF
                     VQSYGSPILDASLLMIPLVGFLPADDPRVRGTIEAIERDLTVDGLVLRYPEGEESADV
                     DGLPPGEGTFLLCSFWLADCLHLLGRDDDARALFERLLSLRNDVGLLAEEYDTRAGRL
                     LGNFPQAFSHIGLVNTAHNLSGARGPADERCAEEEDYAGGRSS"
BASE COUNT          307 a          898 c          886 g          330 t
ORIGIN      
        1 gtcgggttct tccggctcct cggcggcgtc ggcgcggcgt gcgatgcgat cgccgactgg
       61 ggtcgctcgt cgtcccggaa gcgtgtggcc cgcgtcgcgc cccgctacgc cggccggatc
      121 gaccgcacgc ccggccgcca cggcgcgaag cggatgtgac ggtccgccgc ccggccgccg
      181 ctcgcgcgcc tttcgtgacg gttgcgcgcg gcctccgtca cggggtctga ccccgtaagc
      241 gcgggcgccg cgcggcctcc ccagcacccc ggagcgcgaa gaacggggcg atttgcaggg
      301 acgtttgcct tcggcgcgaa ggcatgcgcg ttcggcgacg gggtctgacc ccgtgaaggc
      361 gggcgcgtca cacttccgtg acggtttccg ggcgcgggaa accccgctcc gagcagggac
      421 gttacgaagt cggcacacat tcgtatgcgg atcggtgcgg ggtctgaccc cctttcggcg
      481 accgacccgc tacgcgcgcg cgaggcctcg acgcactccg gcgttcccgc ccgccggaaa
      541 gcggacacta cgaggccatg gcgctcccgc tcgaggacta cgccctcgta ggcgacacgc
      601 aggcagccgc gctcgtcggc cgcgacggct cgatcgactg gctctgcgtc ccgcgcttcg
      661 actcgcccgc gtgcttcgcc gcgctcctcg gcacgcgcga gcacggacgc tggctcctcg
      721 cacccgccgg cggcccgccc gcgaccacgc gccgctacgc gggcgactcg ctcgtcctcg
      781 agaccgactt cgagacggag gacggagccg tccgcgtcac cgacttcatg ccgccgcgcc
      841 aggccgcgcc cgacctcgtg cgcatcgtcg aggggctccg cgggcgcgtc gcgatgagga
      901 tggagctcgt cgtccgcttc gactacggct cgatcgtccc gtgggcccgc cgcctgcagg
      961 aggcggtgtc gtacgtcggc ggccccgacg cgctcgccct cgagacgccg gtcgagacgc
     1021 acggcgagaa cctcaccacc gtcgccgagt tcacggtcgc ggagggcgag cgcgtcccgt
     1081 tcgtgctggc gtggcatccg tcgcacgagc ccccgccgcg ctcggtcgac gccgagcagg
     1141 cgctccgcga gacgctcacc tggtgggagg aatgggcggc ccggtgcacg tacgagggcg
     1201 agtggcgcga cgcggtgctg cgctcgctca cggtcctcaa ggcgctcacg tacgcgccga
     1261 ccggcgggat cgtcgccgcg ccgacgacgt cgatcccgga ggcgatcggc ggcgtgcgca
     1321 actgggacta ccgctactgc tggctccgcg acgcgacgtt cacgctctac gcgctcctga
     1381 gctcgggcta cacgaacgag gcgcgcgcgt ggcgggactg gctcgtccgc gccgtcgcgg
     1441 gcgacccggc gcagctccag atcatgtacg gcctcggcgg cgagcggcgc cttcgcgagc
     1501 acgagctcga ctggctcccg ggatatgagg gctcgcgacc cgtgcgcatc gggaacgccg
     1561 cgagcgcgca gctccagacg gacgtctacg gcgaggtgat ggacgcgctc cacaacgccc
     1621 gccgagtgga gatcgagccg agcgacacgg cctgggcgct ccagcgcgtg ctcctcgagg
     1681 agctcgagcg cgagtggacg ctcccggacg aggggatctg ggaggtgcgc ggcccccgcc
     1741 ggcacttcac gcactcgaag gtgatggcct gggtcgggtt cgaccgggcc gtgaaggcgg
     1801 tcgagcgctt cggccgcgac ggcgacgccg agcgctggaa gcggctgcgc gccgagatcc
     1861 acgacgaggt ctgccgcgag ggctacgacg ccgggcgggg cgcgttcgtc cagtcgtacg
     1921 gctcgccgat cctcgacgcg agcctgctca tgatcccgct cgtcggcttc cttccggcgg
     1981 acgacccacg tgtgcgcggc acgatcgagg cgatcgagcg ggacctgacg gtggacgggc
     2041 tcgtgctgcg ctatccggag ggcgaggagt ccgccgacgt cgacggcctg ccgcccggcg
     2101 aggggacgtt cctcctctgc tccttctggc tcgccgactg cctccacctg ctcgggcgcg
     2161 acgacgacgc gcgggcgctg ttcgagcggc tcctctcgct caggaacgac gtcggtctcc
     2221 tcgccgagga gtacgacacg cgcgccggcc ggctcctcgg caacttcccg caggcgttct
     2281 cccacatcgg gctcgtgaac accgcgcaca acctctcggg cgcccgcggc ccggcggacg
     2341 agcgctgcgc ggaggaggag gactacgccg gcggccgctc gtcgtagcgg gggaggccgt
     2401 cgtccgggat cggctcccag g
//
LOCUS       DASTFC010000150         4126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1172704,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000150 DASTFC010000000
VERSION     DASTFC010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172704"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFO88_04505"
     CDS             complement(<1..569)
                     /locus_tag="VFO88_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9544816.1"
                     /translation="MPAALELRNLTKRYGNVVGVHDLSLDLEPGEVLGFLGPNGAGKT
                     TTIRCVLDMIRPTEGRIRVLGLDAREGSVAIRRRLGYLPGDLVLYERLTARELLRYFG
                     RLRGGVEAGRMEALADRLELVLDRPIRQLSSGNRQKVGVLQAFMHDPELLVLDEPTSE
                     LDPLMQAAVHELVREAAGRGAAVFFSSHQL"
     gene            594..1322
                     /locus_tag="VFO88_04510"
     CDS             594..1322
                     /locus_tag="VFO88_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9544817.1"
                     /translation="MGSVRIGCSGWNYAHWRERVYPAGLPQRRWLEHYATLFDTVEVN
                     ATFYRLPRRDSVARWVEQTPPGFLFSVKASRYLTHVKRLTDLGPGLERFYACIEPLVE
                     SPKLGPVLWQLPGNFHRDDDRLAAALERLPGGRHCFEFRHASWFVPDVFELLRAHGVA
                     LVIGDAPERPFQTEDRTADWTFVRFHYGRRGRRGNYSERELDDWAGRIARHAALGDAY
                     VYFNNDWEGFAVRNAHGLIRRLVE"
     gene            1390..1746
                     /locus_tag="VFO88_04515"
     CDS             1390..1746
                     /locus_tag="VFO88_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544818.1"
                     /translation="MATRVLIVDDHPSFRAMARALLEAEGFEVVGEAETGIAGLRAAK
                     ELQPDLVLLDVQLPDFDGFEVAARLTSANGDSPAVVLTSSRDASDFGPLVAESGAKGF
                     VPKAELSGGALATLIE"
     gene            1743..>4126
                     /locus_tag="VFO88_04520"
     CDS             1743..>4126
                     /locus_tag="VFO88_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9544819.1"
                     /translation="MSARSAVVLWTLALVAGVYAFVLAVTSPHPDEVTVRSVLSTVAG
                     LSFVGSGLVLRLRRPDTRMGTIMVIAGFSWFLPALAQSGHAVPFTIGMGLNDAAWAFF
                     AWVLLAYPSGRVEGLAAKTLVAGAFVLGLVLRPIWILFTDLDRVHPDGPGNAFLVADR
                     EDVSLNISHVIEGLALALIVGAIAVLVQRWRSASPPLRRTLAPPFLAFGVTISLLAVA
                     VILQVLGMWASWIFYWAALVALLTVPLSFAVGLLQTRLARAGVGRLLVELDDVAEAED
                     LRAALARALGDPSLQLAFWLPEHRSFVDADGDPVEPPLGGNGDGARLATVVERAGEPV
                     AALIHDASLASDPGLLEAVSAAAGLSLENERRLTALMRSEARLRALVEALPDLMFRLS
                     REGRYLDVQAPETSLLAAPRDELLGRTVHEALPERVAAQLLEAVDRLRSPGDVESVEY
                     SLELNGVERYFEARLSPIGAEEVVLIVRDISERRAHELELSNLQAELRARLGELDRER
                     ELSRAVVQTSPSFFCILDGGGRIVRFNVALERATGLVDDDRVRGRPFWEVFPIPEHRE
                     RVREAIATASVDEREDCWVSATGEHIDVAWSLTYLPAEQGRLRYLVTGMDVTERARHE
                     AELRRSRARIVEAASVERRRLERNLHDGAQQRLVSLSLSLRLAQARVRTDPEQAETQL
                     QSASDELGHALEELRELARGIHPAVLTDRGLEPALQSLVTRAPVPVTLAEAPEERLPE
                     PVEAAAYYVVAEALTNVAKYAEASAATVRVSRVNGAAVVEVADDGIGGADPGNGS"
BASE COUNT          492 a         1502 c         1519 g          613 t
ORIGIN      
        1 agctggtgcg aggagaagaa gaccgcggcg ccgcgccccg ccgcctcgcg gacgagctcg
       61 tgcacggccg cctgcatgag cgggtcgagc tcgctcgtcg gctcgtcgag cacgagcagc
      121 tccgggtcgt gcatgaacgc ctggaggacg ccgaccttct ggcggttccc gctcgagagc
      181 tggcggatcg gccggtcgag gacgagctcg aggcggtcgg cgagcgcctc catgcgcccg
      241 gcctcgacgc cgccgcgcag ccggccgaag tagcggagga gctcccgggc cgtcagccgc
      301 tcgtagagca cgaggtcgcc cggcaggtag ccgagccgcc gcctgatcgc cacgctcccc
      361 tcgcgcgcgt cgagcccgag cacgcggatt cgcccctcgg tcgggcggat catgtcgagg
      421 acgcagcgga tcgtcgtcgt cttccccgcc ccgttcgggc ccaggaagcc gagcacctcg
      481 cccggctcga ggtcgagcga gaggtcgtgg acgccgacga cgttcccgta ccgcttcgtc
      541 aggttccgga gctcgagcgc ggcgggcacc gggcaacgct accgtgcccg ccgatgggga
      601 gcgtccggat cggctgctcc ggctggaact atgcgcactg gcgcgagcgc gtctacccgg
      661 ccgggctccc gcagcggcgc tggctcgagc actacgcgac gctcttcgac acggtcgagg
      721 tgaatgcgac cttctaccgc ctgccgaggc gcgactcggt cgcccgctgg gtggagcaga
      781 cgccgcccgg cttcctcttc tcggtgaagg cgagccggta cctgacgcac gtgaagcgcc
      841 tgaccgacct cgggcccggg ctcgagcgct tctacgcgtg catcgagccg ctcgtcgagt
      901 cgccgaagct cggcccggtg ctctggcagc tccccgggaa cttccaccgc gacgacgacc
      961 ggctcgccgc cgcgctcgag cggctgcccg gcgggcggca ctgcttcgag ttccgccacg
     1021 cgtcgtggtt cgtgccggac gtcttcgagc tcctgcgcgc gcacggcgtc gcgctcgtga
     1081 tcggcgacgc gcccgagcgg ccgttccaga cggaggaccg gacggcggac tggacgttcg
     1141 tgcgcttcca ctacgggcgc cggggccggc gagggaacta ctcggagcgg gagctcgacg
     1201 actgggcggg ccggatcgcg cgccacgcgg cgctcggcga cgcgtacgtc tacttcaaca
     1261 acgactggga gggcttcgcc gtccgcaacg cgcacgggct catacgccgt ctggtcgagt
     1321 agttgtccgc agtcagaggt gtggatagcc gtatgccgct tccgggtacg tcgtgcgacg
     1381 atgctgttga tggcaaccag ggttctcatc gtcgacgacc acccgagctt ccgggcgatg
     1441 gcccgggcgc tcctcgaggc agagggcttc gaggtcgtcg gcgaggcgga gaccgggatc
     1501 gccgggctgc gggccgcgaa ggagctccag cctgacctcg tcctgctcga cgtccagctc
     1561 cccgacttcg acgggttcga ggtcgcggcg cgcctcacga gcgcgaacgg cgactccccg
     1621 gccgtcgtgc tcacgtcgag ccgcgacgcc tccgatttcg gcccgctcgt cgccgaaagc
     1681 ggcgcgaaag gtttcgtgcc aaaagctgag ctttcgggag gagcgctcgc tactctgatc
     1741 gagtgagcgc caggagtgcg gtcgttctgt ggacgctggc cctcgtggcc ggcgtgtacg
     1801 cgttcgtcct tgcggtgacg agcccccatc cggacgaggt cacggtgcgc tccgtgctct
     1861 cgacggtcgc ggggctctcg ttcgtcggct ccggcctcgt cctcaggctg cgccgcccgg
     1921 acacgcgcat ggggacgatc atggtgatcg cgggcttctc gtggttcctg ccggcgctcg
     1981 cgcagtcggg ccacgccgtc ccgttcacga tcgggatggg cctgaacgac gccgcctggg
     2041 cgttcttcgc gtgggtgctc ctggcgtacc cgtccgggcg ggtcgagggg ctcgcggcga
     2101 agacgctcgt cgcgggcgcg ttcgtcctcg gcctcgtcct ccggccgatc tggatcctct
     2161 tcaccgacct cgaccgcgtc cacccggacg ggccgggcaa cgcgttcctc gtcgccgacc
     2221 gggaggacgt ctcgctgaac atctcgcacg tgatcgaggg gctcgcgctc gcgctcatcg
     2281 tcggcgcgat cgcggtgctc gtccagcggt ggcggtccgc gtccccgccg ctgcgccgga
     2341 cgctcgcgcc gccgttcctc gcgttcggcg tcacgatctc gctcctcgcc gtcgcggtga
     2401 tcctccaggt gctcgggatg tgggcctcgt ggatcttcta ctgggccgcg ctcgtcgcgc
     2461 tcctcaccgt gccgctttcg tttgcggtcg ggctgctgca gacgcggctc gcgcgcgccg
     2521 gcgtcggtcg gctgctcgtc gagctcgacg acgtcgccga ggcggaggac ctgcgcgccg
     2581 cactcgcgcg ggcgctcggc gacccgtcgc tccaactcgc gttctggctc ccggagcacc
     2641 gctcgttcgt cgacgcggac ggcgacccgg tcgagccgcc cctgggcgga aacggggacg
     2701 gcgcgcggct cgccaccgtg gtcgagcggg ccggcgagcc ggtcgcggca ctgatccacg
     2761 acgcgtcgct cgcctccgac cccgggctgc tcgaggcggt cagcgccgcc gccgggctct
     2821 cgctcgagaa cgagcgccgg ctcaccgcgc taatgcgctc ggaggcgcgg ctgcgcgccc
     2881 tcgtcgaggc gctgccggac ctcatgttcc gcctgtcgcg tgaggggcgg tacctcgacg
     2941 tccaggcgcc cgagacgtcc cttctcgcgg caccgcgcga cgagctgctc gggcggacgg
     3001 tgcacgaggc gctcccggag cgggttgccg cgcagctcct cgaggcggtc gaccgccttc
     3061 gctcgcccgg cgacgtcgag tcggtcgagt actcgctcga gctgaacggc gtcgagcgct
     3121 acttcgaggc ccgcctctcg ccgatcgggg ccgaggaggt cgtgctcatc gtccgcgaca
     3181 tctccgagcg gcgggcgcac gagctcgagc tctcgaacct ccaggcggag ctgcgcgcgc
     3241 ggctcggcga gctcgaccgc gagcgcgagc tctcccgcgc ggtcgtgcag acgtcgccga
     3301 gcttcttctg catcctcgac ggcggcgggc ggatcgtgcg cttcaacgtg gcgctcgagc
     3361 gggcgaccgg gctcgtggac gacgaccgcg tgcgcgggcg gccgttctgg gaggtcttcc
     3421 ccatccccga gcaccgcgag cgggtgcgcg aggcgatcgc gaccgcgtcg gtggacgagc
     3481 gggaggactg ctgggtctcg gcgacgggcg aacacatcga cgtcgcgtgg tcgctcacat
     3541 acctccccgc cgagcagggc cggctgcggt acctcgtgac cgggatggac gtgaccgagc
     3601 gcgcccgcca cgaggcggag ctgcgccgct cgcgcgcccg gatcgtcgag gcggcgagcg
     3661 tcgagcggcg ccggctcgag cggaacctcc acgacggggc gcagcagcgg ctcgtgtcgc
     3721 tctcgctctc gctccggctc gcccaggcgc gggtccggac ggatcccgag caggcggaga
     3781 cgcagctcca gtcggcgagc gacgagctcg ggcacgcgct cgaggagctt cgcgagctcg
     3841 cgcgcggcat tcacccggcg gtgctcacgg accgcggcct cgagccggcg ctccagtccc
     3901 tcgtgacgcg cgcgcccgtg ccggtcacgc tcgcggaggc gccggaggag cgcctcccgg
     3961 agccggtcga ggcggccgcg tactacgtcg tcgccgaggc gctcacgaac gtggcgaagt
     4021 acgcggaggc gtccgcggcc accgtgcggg tctcgcgcgt gaacggcgcc gcggtggtcg
     4081 aggtcgccga cgacgggatc ggcggcgccg acccggggaa cggctc
//
LOCUS       DASTFC010000151         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_645460,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000151 DASTFC010000000
VERSION     DASTFC010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645460"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFO88_04525"
     CDS             <1..317
                     /locus_tag="VFO88_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544820.1"
                     /translation="SRALRQDSGVGRASDWLREERRKTLGDWVAFCLACGHAQRYFTE
                     TEIDVPTACPQCGGEIRSRCPSCSARVSSMFAVECEECGAELRPPVQFGATIRKPPKK
                     RG"
     gene            complement(314..691)
                     /locus_tag="VFO88_04530"
     CDS             complement(314..691)
                     /locus_tag="VFO88_04530"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET9544821.1"
                     /translation="MYALNFYSPMVADQLRSRRKTATIRLGDKSPKYKKGMVVAVLVG
                     TRFGPREKVFDAVIDKIEVKPLLDLSPREIEHDNPEIRRPEEMATFLSRLYNREIGPE
                     DTVTVIHFSEIVDPSGLAASLPG"
     gene            complement(720..1178)
                     /locus_tag="VFO88_04535"
     CDS             complement(720..1178)
                     /locus_tag="VFO88_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9544822.1"
                     /translation="MRREFSAGGVVVRRIRGEWRLAAIRPGGKPEGTWALPKGLVGRD
                     EDPAATALREVHEETGLRGTLAGKLGDVRYVYTWAGERIFKIVSFYLVRAPRGRLGDL
                     PPEQAFEVAETRWLPLAEAPRLLAYKGEREMAERALEALAGAKAGASKSA"
     gene            1228..2388
                     /locus_tag="VFO88_04540"
     CDS             1228..2388
                     /locus_tag="VFO88_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544823.1"
                     /translation="MRRLLVLVCGIVLVDTMLYAALTPLLPEFSDDFGLSKGGAGLLV
                     AVYAVGVLAGAVPAGLAAARFGPKRVALGGLVVVALASIVFGFAGDVWTLGAARFVQG
                     LGSALSWAGGLAWLISASPRGRRGELLGTALGAAIFGALLGPALGALAAEIGTAPTFT
                     ALGVGALAIGFFGLRVPGVAGEPPTLAPLRAAFGERRFVGGLWLMTLPALLFGVLAVL
                     VSLDLDRLGWSSRAIGAVFVATAAVEAVIAPLVGRFVDRRGALPPVRLGLTAGIGVAL
                     GLAWAGDPFVIAGLALLASVSWGTLFTPGMAMLSQGAEDVGLPQALAFGLMNAAWAGG
                     AMVGPGVGGAVGGAFGDAAAYGLGAVACAATLVVLTRLAPIGTRLSRVPTNP"
     gene            2480..2785
                     /gene="groES"
                     /locus_tag="VFO88_04545"
     CDS             2480..2785
                     /gene="groES"
                     /locus_tag="VFO88_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9544824.1"
                     /translation="MDLKPLGDRLIVEVLEDEETTDSGIVLPDTAKEKPQRGRVLAVG
                     PGGRNEDGDYMPIDLAEGDEIIFSKYGGTEVRLGTEDYLILRESDVLAKVVLAGAPA"
     gene            2810..>3396
                     /locus_tag="VFO88_04550"
     CDS             2810..>3396
                     /locus_tag="VFO88_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HET9544825.1"
                     /translation="MAHKDLKFNEEARRSLERGVNVLADAVKVTLGPKGRYVVLDKKF
                     GAPTITNDGVTIAREIELEDVFENQGAQLVREVATATNDVAGDGTTTATVLAQAIVRE
                     GLKNVAAGANPMALKRGIEKAVEQVVEDLKSQSTEISGKEDIARVATISSREREIGDV
                     IADAIEKVGKDGVVNVEEGQTFGLELEFTEGMQFD"
BASE COUNT          437 a         1190 c         1259 g          510 t
ORIGIN      
        1 cctcgcgcgc gctccggcag gattcgggcg tgggacgtgc ctccgactgg ctgcgcgagg
       61 agcggcgcaa gacgctcggc gactgggtcg cgttctgtct cgcgtgcggt cacgcgcagc
      121 gctacttcac ggagacggag atcgacgtgc ccaccgcctg cccacagtgc ggcggcgaga
      181 tccgcagccg ctgcccgtcc tgctcggcgc gcgtgagctc gatgttcgcg gtcgagtgcg
      241 aggagtgtgg cgccgagctc cgcccgcccg tccagttcgg tgcgacgatc cggaagccgc
      301 cgaagaagcg gggctagccg ggcagcgacg cggcgagccc ggacgggtcg acgatctcgg
      361 agaagtggat gaccgtaacg gtgtcctccg gccctatctc gcggttgtag aggcggctga
      421 ggaaggtcgc catctcctcc ggcctgcgga tctccgggtt gtcgtgctcg atctcgcgcg
      481 gcgagaggtc gagcagcggc ttcacctcga tcttgtcgat caccgcgtcg aagaccttct
      541 cccgcgggcc gaagcgcgtc cccacgagca cggccacgac catccccttc ttgtacttcg
      601 gggacttgtc gcccagccgg atcgtcgccg tcttccggcg ggagcggagc tggtcggcga
      661 ccatcgggga gtagaagttc agcgcgtaca cggcggaggt gaggccggat gatagagggt
      721 cacgcgcttt tcgacgcccc ggccttcgcg cctgccagcg cctcgagggc gcgctcggcc
      781 atctcgcgct cgcccttgta ggcgaggagg cgcggcgcct ccgcgagtgg gagccagcgc
      841 gtctcggcga cctcgaaggc ctgctcgggc gggaggtcgc cgagccgtcc gcgcggcgcg
      901 cgcacgagat agaagctcac gatcttgaag atgcgctcgc ccgcccacgt gtagacgtag
      961 cggacgtcgc cgagcttgcc cgcgagcgtt ccgcggagcc cggtctcctc gtgcacctcg
     1021 cggagcgccg tcgcggccgg gtcctcgtcc cgcccgacca ggcccttcgg cagcgcccat
     1081 gttccctccg gcttgccgcc cgggcggatc gccgcgaggc gccattcgcc ccggatccgg
     1141 cggacgacca caccgcccgc cgagaactcg cgcctcatgc cgccgatcgt agacgcggag
     1201 ccgcgttcgg caggtagcgt cgcgcccgtg cgccggctcc tcgttctcgt ctgcgggatc
     1261 gtcctcgtgg acacgatgct ctacgcggcg ctcacgccgc tcctgcccga gttctcggac
     1321 gacttcgggc tctcgaaggg cggcgcgggg ctgctcgtcg cggtctacgc ggtcggcgtg
     1381 ctcgcgggcg cggtgccggc ggggctcgcc gccgcgcgat tcgggccgaa gcgagtcgcg
     1441 ctcggcgggc tggtcgtcgt cgcgctcgcg agcatcgtgt tcgggttcgc cggcgacgtc
     1501 tggacgctcg gcgccgcgcg gttcgtccag gggctcggga gcgccctctc ctgggcgggc
     1561 gggctcgcgt ggctcatctc ggcgtcgccg cgcgggcggc ggggagagct gctcggaacg
     1621 gcgctcggcg cggcgatctt cggcgcgctc ctcgggccgg cgctcggcgc cctggcggcg
     1681 gagatcggca cggcgccgac gttcacggcg ctcggcgtgg gcgcgctcgc gatcggcttc
     1741 ttcggcctcc gcgtccccgg ggtcgccggg gagccgccga ccctcgcgcc gcttcgcgcc
     1801 gcgttcgggg agcggcgctt cgtcggcggg ctctggctca tgacgctgcc ggcgctcctc
     1861 ttcggcgtgc tcgccgtgct cgtctcgctc gacctcgacc ggctgggctg gagctcgcgc
     1921 gcgatcggcg ccgtgttcgt cgcgacggcg gcggtcgagg cggtgatcgc cccgctcgtc
     1981 ggccgcttcg tcgaccggcg cggcgcgctg ccgccggtcc ggttgggcct gaccgccggg
     2041 atcggcgtcg cgctcgggct cgcgtgggcc ggcgacccct tcgtgatcgc cggcctcgcc
     2101 ctcctcgcgt cggtgtcgtg gggaaccctc ttcacgcccg ggatggcgat gctctcccag
     2161 ggcgccgagg acgtcggcct gccgcaggcg ctcgccttcg ggctcatgaa cgccgcgtgg
     2221 gcgggcggcg cgatggtcgg ccctggggtg ggcggcgcgg tcggcggcgc cttcggcgac
     2281 gcggccgcct acgggctcgg cgcggtggcg tgcgccgcga cgctcgtcgt cctcacgcgg
     2341 ctcgccccta ttggcactcg tctctcgcga gtgccgacaa acccttgatc gagtcgaccg
     2401 cgtttcgcta ccatggctcc tggcactcgg atgggccgag tgccaggagc gacagacatc
     2461 gctggcaaag gaggcttgca tggacctgaa gccgcttgga gatcgcctga tcgtcgaggt
     2521 cctcgaggac gaggagacga cggacagcgg catcgtcctg cccgacaccg cgaaggagaa
     2581 gccgcagcgc ggccgcgtcc tcgccgtcgg cccgggcggg cggaacgagg acggcgacta
     2641 catgccgatc gacctcgccg agggcgacga gatcatcttt tccaagtacg gcggcacgga
     2701 ggtgcggctc gggacggagg actacctgat cctccgcgag tccgacgtgc tcgcgaaggt
     2761 cgtcctggcg ggcgcgcccg cctagcggaa cagacgaagg aggaagtgca tggcgcacaa
     2821 ggatctgaag ttcaacgagg aggcacggcg ctcgctcgag cggggcgtga acgtcctggc
     2881 cgacgcggtc aaggtcacgc tcggcccgaa gggccgctac gtcgtcctcg acaagaagtt
     2941 cggtgccccg acgatcacga acgacggcgt cacgatcgca cgcgagatcg agctcgagga
     3001 cgtcttcgag aaccagggcg cgcagctcgt ccgtgaggtc gcgaccgcga cgaacgacgt
     3061 cgccggcgac ggcacgacga ccgcgaccgt gctcgcgcag gcgatcgtcc gcgagggcct
     3121 caagaacgtg gccgccggcg cgaacccgat ggcgctcaag cgcggcatcg agaaggccgt
     3181 cgagcaggtc gtcgaggatc tcaagtcgca gtcgacggag atctcaggca aggaggacat
     3241 cgcccgcgtc gccaccatct cgtcgcgcga gcgcgagatc ggcgacgtga tcgccgacgc
     3301 gatcgagaag gtcggcaagg acggcgtcgt gaacgtcgag gagggccaga ccttcggcct
     3361 cgagctcgag ttcaccgagg gcatgcagtt cgacaa
//
LOCUS       DASTFC010000152         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1090806,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000152 DASTFC010000000
VERSION     DASTFC010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090806"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1031
                     /locus_tag="VFO88_04555"
     CDS             291..1031
                     /locus_tag="VFO88_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9544826.1"
                     /translation="MASVLVIRSGEGETITDRPERTIRVLVEHEALTLSLFRYEPGQK
                     GPDPHVHRRHTDAFYVLDGELELGLGPEVERIVGTPGTLAAAPPNVVHTFRNASDRMT
                     LFLNVHTPSMGFAAMLRAAHEGGDEDAAHFDEYDPPEDGGRQLADAVLRGPGEGRRTG
                     LDGMTTLAKSEGADADGQLSLVEVELEPGATYADPAAGQVDVLFMLDGTVSLGVDCEA
                     RPGDVVVGPGSVRNDGSSDARLLHLGSA"
     gene            complement(1043..1114)
                     /locus_tag="VFO88_04560"
     tRNA            complement(1043..1114)
                     /locus_tag="VFO88_04560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1079..1081),aa:Arg,seq:cct)
     gene            1210..2613
                     /locus_tag="VFO88_04565"
     CDS             1210..2613
                     /locus_tag="VFO88_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544827.1"
                     /translation="MRRGSDPRATAGGARWRDTLAPGLLLLVVLVAFAARENVGSEDR
                     FDRSVSRYPHALPSAELSDGLTTYPVTSVERTAATANATVVGGEPGARGFVVSDALPS
                     IGIAAGGDLGSPALSTERVRRELAGYLALGVGQVRADAPWAQIERTPGVYDWSWPDRW
                     IPEAHALGLDVVVTFACSPPWVTGAAASDKVGPATAEERDAYAAAAAALVRRYPYVTA
                     IEIWNEPNHAGFWAAPDPAAYADLLRRAHAAIKAVAPGIQVLGGVLALPPPGEGVMPV
                     PFLEGMYAAGAHGAFDALAVHPYIGNDESIYWESWNAFDGSGSWFGPAPSVLAVLQAN
                     EPGKRIWFTETGDTALAATSPATVPKILDKLREYQSAGWGGPIFWYAWRSIDSYSFVD
                     ENMSPRPAYAVFRDYLASVFAGDASRTFDARAGADGAYSAALTGLEPGTTYWVRAYAS
                     DGSRTRYGSVWRFRTAH"
     gene            2653..2949
                     /locus_tag="VFO88_04570"
     CDS             2653..2949
                     /locus_tag="VFO88_04570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9544828.1"
                     /translation="MDGDLRVIDRPAELAYRLYVGEREAGELRYARQGDRLVLVHTEV
                     DRSLGRQGLGSALVRGALDDVRARGLEIVPRCPFVRRYLERHPEYTDLVADPES"
     gene            complement(2955..>4192)
                     /locus_tag="VFO88_04575"
     CDS             complement(2955..>4192)
                     /locus_tag="VFO88_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9544829.1"
                     /translation="ETLVFRAATAVALVHALDDSFLGRQPGVPLGQHALAAALALVLG
                     GAALVWFPRLRPVLAALVSLVFGVLAIGNGALHLIHVALDGPAASDLTGIAAFAAGIV
                     LVLLAPTILFRHRGERARSARRRWTLRALGVIGLALTAYAILFPLTGAIIQTHKFREP
                     VGDPPSAAYEPVTFTAEDGLELSGWYVPSENGAAVVVVHGGGGDRMGTLDHAELLARN
                     GYGVLVYDSRGRGESEGSHNGFGWDWEKDVDGALDFLHERPDVDPERIGGIGLSTGAE
                     VLLEVAPERRDLQAVVADGLTARSFDDYRNLDGLTEAAPFYWTMMAGARVFSGSSPGP
                     PLKELAAEVSPTPLLLVSTGVGIATEIDFNRLYAEAAREPVELWELPDVAHTAAVRER
                     SDEYEERVIGFLDEALAQR"
BASE COUNT          577 a         1483 c         1588 g          544 t
ORIGIN      
        1 gccgcccgcg cggtagagga ggtacgcgaa cccgggcacg gcgaccccgt agcgcacgag
       61 cgcgagatcc tccccggcga agccggagtc gagcaggacc ttgacggcgg cgaacgccga
      121 ggcccacgcg acgatcgcgc cgacgagagc tgcctgaaca cgcggctgca tacccgtatc
      181 caacgagatt gccgcggccg gcccgcacct ggtggcacga aaatcgtcgt acggacagcc
      241 gtacttccgc gctgcggttc tctggctcgc cgggcgggga ggacgaggct atggccagcg
      301 tcctcgtgat ccggtccggt gagggcgaga cgatcaccga ccggccggag cggacgatcc
      361 gcgtgctcgt cgagcacgag gcgctgacgc tcagcctctt ccgctacgag cccggccaga
      421 aggggcccga cccgcacgtg caccggcggc acacggacgc gttctacgtc ctcgacggcg
      481 agctcgagct cggcctgggg ccggaggtcg agcgcatcgt cggcacgccg ggcacgctgg
      541 ccgccgcgcc gccgaacgtc gtgcacacgt tccggaacgc gagcgaccgg atgacgctct
      601 tcctgaacgt ccacacgccg agcatgggct tcgccgccat gctccgagcg gctcacgagg
      661 gcggcgacga ggacgccgcg cacttcgacg agtacgaccc gccggaggac ggcggccggc
      721 agctcgcgga cgcggtgcta cgcgggccgg gcgaggggag gcggacgggg ctcgacggga
      781 tgacgacgct cgcaaagtcg gagggcgccg acgccgacgg acagctctcg ctcgtggagg
      841 tcgagctcga gccgggcgcg acgtacgcgg atcccgccgc tgggcaggtc gacgtcctct
      901 tcatgctcga cgggacggtg tcgttggggg tcgattgcga ggctcggccg ggggatgtcg
      961 tcgtggggcc cggctcggtg cggaacgacg gctcttccga cgcgcggctc ctccatctcg
     1021 gctcggcgtg agccacgcgg gatgcccccg gcaagaatcg aacttgcgca cgcggtttag
     1081 gaaaccgctg ctctatccac tgagctacgg gggctcgggt ccagtgtaga gccggttccc
     1141 tcggggtcgg cgctcgcgcg cgggagcagg caccatgggc gctcaggcgg cgaaccaggc
     1201 gggggtgcgg tgaggcgcgg gagtgatccg cgagcgacgg caggcggggc gcgatggagg
     1261 gacacgctcg ctccgggcct gctcctgctc gtcgtcctcg tcgccttcgc ggcgagggag
     1321 aacgtcgggt cggaggaccg gttcgatcgt tctgtctccc ggtacccgca cgcccttccg
     1381 agcgcggagc tctccgacgg cctcacgacg tatcccgtga cgtcggtcga gcgcacggcg
     1441 gcgacggcga acgcgacggt cgtcggcggc gagccggggg cccgcgggtt cgtcgtgtcg
     1501 gacgcattgc cgtcgatcgg gatcgctgcg ggcggcgatc tgggctcgcc cgcgctgtcg
     1561 accgagcgcg tccggcggga gctcgccggg tacctcgcgc tcggcgtcgg ccaggtccgc
     1621 gccgacgcgc cctgggcgca gatcgagcgc acgcccggcg tctacgactg gtcgtggccc
     1681 gaccggtgga tcccggaggc ccacgcgctc gggctcgacg tcgtcgtgac gttcgcctgc
     1741 tcgccgccgt gggtcaccgg tgcggccgcg agcgacaagg tcgggcccgc gaccgcggag
     1801 gagcgtgacg cgtacgcggc cgcggcggcc gcgctcgtcc gtcgctaccc gtacgtcacg
     1861 gcgatcgaga tctggaacga gccgaaccac gccggcttct gggccgcgcc ggacccggcg
     1921 gcctacgccg atctgctgcg gcgcgcgcac gcggcgatca aggccgtcgc gccgggcatc
     1981 caggttctcg gcggcgtcct ggcgcttccg ccgccggggg agggcgtcat gccggtgccg
     2041 ttcctcgagg ggatgtacgc ggccggcgcg cacggcgcgt tcgacgcgct cgccgtccac
     2101 ccgtacatcg gcaacgacga gagcatctac tgggagtcgt ggaacgcgtt cgacgggtcc
     2161 ggctcgtggt tcgggcccgc gccgtcggtg ctcgccgtgc tgcaggcgaa cgagccgggg
     2221 aagcggatct ggttcacgga gacgggggac acggcgctcg ccgcgacgtc gcccgcgacg
     2281 gtcccgaaga tcctcgacaa gctccgggag taccagtcgg cgggctgggg cgggccgatc
     2341 ttctggtacg cgtggcgctc gatcgactcc tactcgttcg tcgacgagaa catgagccca
     2401 aggcccgcgt acgcggtctt tcgcgactac ctcgcctccg tcttcgcggg cgacgcctcc
     2461 cgcacgttcg acgcccgggc cggcgcagac ggcgcgtact cggcggcgct gaccgggctc
     2521 gagcccggga cgacctactg ggtccgcgcg tatgcgagcg acggaagccg cacgagatac
     2581 gggagcgtct ggaggttccg aacggcccac tgagcaggtg ccgtgcgggc ggcgaccgga
     2641 taccgtgacc gtgtggacgg cgatctgcgc gtgatcgacc ggccggccga gctcgcctac
     2701 cggctgtacg tgggcgagcg ggaggccggc gagctccgct acgcccggca gggcgaccgg
     2761 ctcgtgctcg tccacacgga ggtcgaccgg tcgctcgggc ggcaggggct cgggtcggcg
     2821 ctcgtacggg gcgcgctcga cgacgtccgc gcgcgcgggc tcgagatcgt cccgcggtgc
     2881 cccttcgtgc gccgctacct cgagcggcac ccggagtaca ccgacctcgt cgcggatccc
     2941 gagtcgtaat cgcctcagcg ctgcgcgagc gcctcgtcga ggaacccgat cacgcgctcc
     3001 tcgtactcgt cggagcgctc gcgcaccgcg gcggtgtgcg ccacgtccgg aagctcccag
     3061 agctcgaccg gctcccgcgc cgcctcggcg tagagccggt tgaagtcgat ctcggtcgcg
     3121 atcccgactc cggtggagac cagcaggagc ggcgtgggcg agacctccgc cgcgagctcc
     3181 ttgagcggcg ggccgggcga ggatccggag aacacgcggg cgccggccat catggtccag
     3241 tagaacggcg ccgcctcggt gaggccgtcg aggttgcggt agtcgtcgaa cgagcgcgcg
     3301 gtgaggccgt cggcgacgac cgcctgcagg tcgcgccgct cgggagccac ctcgagcagc
     3361 acctccgcgc ccgtcgagag gccgatcccg ccgatgcgct ccgggtcgac gtccggtcgt
     3421 tcgtgcagga agtcgagcgc accgtcgacg tccttctccc agtcccagcc gaagccgtta
     3481 tggctgccct cgctctcgcc gcggccgcgc gagtcgtaga cgaggacccc gtagccgttc
     3541 cgtgcgagca gctcggcgtg gtcgagcgtg cccatccgat cgccgccccc accgtggacg
     3601 acgacgaccg cggcgccgtt ttccgacggc acgtaccagc cggacagctc gagcccgtcc
     3661 tcggccgtga acgtcacggg ttcgtaggcg gcggagggcg ggtccccgac cggctcgcgg
     3721 aacttgtgcg tctggatgat cgcgcccgtc agcgggaaca ggatcgcgta cgcggtgagc
     3781 gcgaggccga tcacgccgag cgccctcagc gtccagcggc gccgcgccga gcgcgcgcgc
     3841 tcgccgcggt ggcggaagag gatcgtcggc gcgaggagga cgagcacgat cccggcggcg
     3901 aaggcggcga tccccgtcag gtcgctcgcc gcaggcccgt cgagcgccac gtggatcagg
     3961 tggagcgcgc cgttgccgat cgcgagcacg ccgaagacga gcgagacgag cgccgcgagc
     4021 acgggccgca gccgtgggaa ccacacgagc gccgcgccgc cgaggacgag cgcgagcgcg
     4081 gccgccagcg cgtgctgccc gaggggcaca cccggctggc ggccgaggaa cgagtcgtcg
     4141 agcgcgtgca cgagcgcgac cgcggtggcc gcccggaaga cgagcgtctc cc
//
LOCUS       DASTFC010000153         6910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1519200,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000153 DASTFC010000000
VERSION     DASTFC010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519200"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFO88_04580"
     CDS             <1..427
                     /locus_tag="VFO88_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9544830.1"
                     /translation="GQRMPVPRDSFVATTWEMLSAGAFLVVLGAVVGELGTVDAGAFS
                     LESVAAWLYLAVFGSLVAFSAYAWLMKNAPISTVVTHQYVNPVVAIALGALVLGERLT
                     VAIGIGAALIVGSVFVAVRQENAARRERSRKAQAAEQPA"
     gene            528..1004
                     /locus_tag="VFO88_04585"
     CDS             528..1004
                     /locus_tag="VFO88_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9544831.1"
                     /translation="MQKITTFLTFDGDAEEAVALYTSIFPDSRVVSERRYGPAGPGPE
                     GSLMTATFELCGQPFMALNGGSSFSFSEGISLFVDCENQAEVDRYWDALTADGGRESM
                     CGWLVDRFGVSWQIIPRALGDLLGDPDPERAGRAMSAMLEMQKIDVATLERAAAGG"
     gene            complement(1174..2910)
                     /locus_tag="VFO88_04590"
     CDS             complement(1174..2910)
                     /locus_tag="VFO88_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544832.1"
                     /translation="MPNRLRTAVLVLAIAVAFADSSIVVLGVPEIVRDFDVAVGDASW
                     VITSYNLAVVAAAAVVFGVLGRFSPRRLAFVGLAAFALASLGCAVAPSFGWLVGFRTA
                     QGLAAALVLVAALPFLGGRAGIRAWILAATVGLAAGPALGGVLTELFSWRSIFAAQAP
                     LVAAGLLALGAAPTVVTGAGRRPRRAWLADATLAAFSAALVGALFLVVVLLINGFGWQ
                     PLPAALVATTLPVAAAVAERIGHGLAPAAAAGAGGVLVACGLTVLAFLPSARTGLVVA
                     GLALCGAGLGLAAQPLGRLALGGPRLEQDAAWTVFFRHAGLVLALVLVTPVLVSSLDA
                     LEQDAEAVAGEVVLRSELPLTEKARVLNDLADAADASEPSVPDVRETLAPREGGEGTI
                     SQVADDLTERLHELIARAFRDPFLLCAAFGLGAALLGLGLGLGEGRVRAPALAFAAVA
                     LVAGAVALGADYRLGALDDPAAGTDPCRAPPAEGGDGLEAAVERIALDSLSGAACELG
                     VSRAAVLRELAGETNDLAPERLEESVRRNVDRALEAETERGTLDGTIGAFLRGILANA
                     PLDWIREALGGL"
     gene            2970..4112
                     /locus_tag="VFO88_04595"
     CDS             2970..4112
                     /locus_tag="VFO88_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544833.1"
                     /translation="MTTPTVEDVRRIAALESPIVRNLEITHCYARLSTAFAARGGAGA
                     NWCTYATWASRQAGRTIRGEDLVDEADRRIHHARWLVRPVATLGRRLLRRGLLRPETR
                     LGRLAAELHTPFDAVELASAAVARGNLKVFEEIGLEFARFLAACPPDANDAALEGFLA
                     GLREGDPPEGQGYLRRAFTRYERARRTDDPRAAAQLTVLANLEIGFHEQTRLQPEILE
                     ALDAPFVTQERLGLRALEALFPSLARAWPVVRAPAAAVAGAFAKGVQRASSRLARELV
                     TESFMTLSLPGRVLLLGRHLEDAFPDALGDPRDAELAELLARFEPAPPARDDCGARDW
                     ADLRQRMHYIVHLFCAFHERDDLFRPPFTPAQVAAFERGLVPDGEL"
     gene            complement(4183..5709)
                     /locus_tag="VFO88_04600"
     CDS             complement(4183..5709)
                     /locus_tag="VFO88_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544834.1"
                     /translation="MGVLTVTAGAQVGDQSFKVQEIFTSPNAATNSDLAFWGNHAFVG
                     YYTGDAAPRGGVRIFDIKNPAAPRLIRDFACDGLQADPIVWDRNGNGVADLLLLAVDR
                     TMANPNCGAPRSAHDDPMGWEGVRVFTMSDNPANPFTNIQQVAAVYTDCGAHTITLWP
                     GEVETQNKLVVYVSSYPLRPGPTCGQLRYNNLANPYDGDPGSPNSPLHGVIQMVEVPL
                     NNPAAATEMNVQPAIAYPGDPDGIFDYCERGLCAPGVLEPASIACHDIVVHVEHRLAG
                     GACAEQGQVWNIGPGGVPDTLNPIMIGDDAFSSGGTGDIPGAVDFFHSVMFNNDATVV
                     NWVDESFGSGCPTMTNWQARPWHPAGPQKTGSMFFSDMETGAFLSEFNVGDVRPNPAP
                     GEYCSAHMGMAVMGIKRDLLVNAWYTGGMDVIDFSKPTRLKEIAYYDPAQNSGTWSAY
                     PYTGPMFKNGPGLPVYATDGVTNNPLAEGMVVYRTIMQKVGKPKRVDHLNPQTMDHME
                     "
     gene            complement(5839..6519)
                     /locus_tag="VFO88_04605"
     CDS             complement(5839..6519)
                     /locus_tag="VFO88_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9544835.1"
                     /translation="MAEYTLNILKPSVNNMSVRVFARAAGLDFEEQDVWGSTTSPEYM
                     AKYPAHLTPMVESPDLPRGALGESCAIMAYLADKHGLENFYPSDPGRRAMVNNAMFYL
                     IGTFYPLLARATYPTLAFGQYPGEVATSEASDELKAQAQKDAEAALAEPLEVFNTFFL
                     DGKPFIGGDHPSIADIRLAATLEFLRAIDYPFPQWTEDFMARMEDNLGAAYTEPAADV
                     RGYVASVK"
     gene            complement(6528..>6910)
                     /locus_tag="VFO88_04610"
     CDS             complement(6528..>6910)
                     /locus_tag="VFO88_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544836.1"
                     /translation="RVASIRDAKEAGYVHHIAFDVSQATFRQVVERLDERGIRHSGVK
                     DRGFMDSIYFEDPLGMLIELASYRFVPPHGFTHPEVLLEAHRLRVERGAYNIDQEHLA
                     DAIEILTARSRESLSDDRSPRNPY"
BASE COUNT          971 a         2501 c         2492 g          946 t
ORIGIN      
        1 cggccagcgg atgccggtgc cgcgcgacag cttcgtcgcg acgacgtggg agatgctgag
       61 cgccggagcc ttcctcgtcg tcctcggcgc cgtcgtcggc gagctcggga cggtcgacgc
      121 gggcgccttc agcctcgagt ccgtcgccgc ctggctctac ctcgcggtct tcggctcgct
      181 cgtcgccttc agcgcctacg cgtggctcat gaagaacgcg ccgatctcga cggtcgtcac
      241 gcaccagtac gtgaaccccg tagtcgcgat cgcgctcggc gcgctcgtgc tcggcgagcg
      301 gctgacggtc gcgatcggga tcggcgccgc gctcatcgtc ggctccgtct tcgtggccgt
      361 ccggcaggag aacgcggccc gccgcgagcg gtcgaggaag gcgcaggccg cggagcagcc
      421 ggcgtaggta aaggggctcg acggccccag gcgcgtctcc cgcctgcgat gagttccgcg
      481 gcgcgcgcgc gtctgtagag gagaatcaac cgagccggga ggaatccatg cagaagatca
      541 cgacgttcct gacgttcgac ggcgacgccg aggaggcggt cgcgctctac acctcgatct
      601 tccccgactc gcgggtcgtg agcgagcggc gctacggccc cgccggcccg ggaccggagg
      661 gctcgctcat gacggccacg ttcgagctct gcgggcagcc gttcatggcg ctgaacggcg
      721 gatcgtcgtt ctcgttctcc gaggggatct cgctcttcgt ggactgcgaa aaccaggcgg
      781 aggtcgatcg ctactgggac gcgctcaccg ccgacggcgg ccgggagagc atgtgcggct
      841 ggctcgtcga ccggttcggc gtctcgtggc agatcatccc gcgggcgctc ggcgacctcc
      901 tcggcgaccc cgacccggag cgggccggcc gcgcgatgag cgcgatgctc gagatgcaga
      961 agatcgacgt cgcgacgctc gagcgagcgg ccgcgggcgg ctaggagggc gcaacgggac
     1021 atcggttcgc cgacggcgtt cgacggacgg cgcgtcgcaa ggacgcaggg agccctcgta
     1081 gcagcgctac gggggcgact gaggacgcag cgaggcggtg tcgggcgggc gccggcggca
     1141 caaacgatgg cccgccgtgt ccttctagcc gccctagagg ccgccgagcg cctcgcggat
     1201 ccagtcgagc ggcgcgttcg cgaggatgcc gcgcaggaac gcgccgatcg tgccgtcgag
     1261 cgtgccgcgc tcggtctcgg cctcgagcgc gcggtcgacg ttcctgcgca cggactcctc
     1321 gaggcgctcc ggcgcgaggt cgttcgtctc gcccgcgagc tcacgcagca cggcggcccg
     1381 gctgacgccg agctcgcatg ccgcgcccga gagcgagtcg agcgcgatcc gctcgaccgc
     1441 cgcctcgagc ccgtcgccgc cctccgccgg cggcgcgcgg cacggatcgg tgcccgcggc
     1501 cgggtcgtcg agcgcgccga gccggtagtc cgcgccgagc gcgaccgcgc cggcgacgag
     1561 cgcgacggcc gcgaacgcga gcgccggcgc gcgcacccgg ccctccccca gcccgagccc
     1621 gaggccgagc agcgccgccc cgagaccgaa cgcggcacag agaaggaacg ggtcgcggaa
     1681 cgcgcgcgcg atcagctcgt gcagccgctc ggtcaggtcg tccgcgacct gcgagatcgt
     1741 cccctccccg ccctcccgcg gcgcgagcgt ctcccgcacg tcggggacgc tcggctcgga
     1801 ggcgtccgcg gcgtcggcga ggtcgttcag gacgcgcgcc ttctcggtca gcggcagctc
     1861 cgagcggagg acgacctccc cggccacggc ctccgcgtcc tgctcgagcg cgtcgagcga
     1921 cgagacgagc accggggtca ccaggacgag cgcgagcacg agcccggcgt ggcggaagaa
     1981 gaccgtccag gccgcgtcct gctcgaggcg cggcccgccg agagcgaggc gcccgagcgg
     2041 ctgcgcggcg agcccgagcc cggcgccgca gagcgcgagc ccggcgacga cgagccccgt
     2101 ccgggccgag ggcaggaagg cgaggacggt gagcccgcac gcgacgagca cgccgcccgc
     2161 cccggccgcg gccgccggcg ccagcccgtg gccgatccgc tccgcgaccg ccgccgccac
     2221 cggcagcgtc gtcgccacga gcgcggccgg gagcggctgc cagccgaacc cgttgatgag
     2281 caggacgacg acgaggaaga gcgcgccgac gagcgccgcg ctgaaggcgg cgagcgtcgc
     2341 gtcggcgagc caggcgcggc gcgggcgccg tcccgcgccc gtcacgaccg tcggggccgc
     2401 gccgagcgcg agcagcccgg ccgcgacgag cggggcctgc gcggcgaaga tcgagcgcca
     2461 ggagaagagc tccgtcagca cgccgccgag ggccgggcct gcggcgaggc cgaccgtcgc
     2521 cgcgaggatc cacgcgcgga tccccgcccg tccgccgagg aacggcagcg cggcgacgag
     2581 gacgagcgcg gccgcgagcc cctgagccgt ccggaacccg acgagccagc cgaaggacgg
     2641 cgcgaccgcg cacccaagcg acgcgagcgc gaacgcggcg agaccgacga acgcgaggcg
     2701 ccgcggcgag aaccgcccga gcacgccgaa caccaccgcg gcggcggcga cgaccgcgag
     2761 gttgtacgac gtgatcaccc acgacgcgtc gccgaccgcg acgtcgaagt cgcgcacgat
     2821 ctcgggcacg ccgaggacga cgatcgagga atccgcgaac gcgaccgcga tcgcgaggac
     2881 gagcaccgcc gtgcgaagcc ggttcggcat ctccgggatg gtgcccgtct cgcgcgcttc
     2941 tcgtcgtggc gacgtgagac gcttcacccg tgacgacgcc gaccgtcgag gacgtccgcc
     3001 gcatcgccgc gctcgagagc ccgatcgtcc ggaacctcga gatcacgcac tgctacgccc
     3061 gcctgtcgac cgcgttcgcc gcccgcggcg gggccggcgc gaactggtgc acgtacgcga
     3121 cctgggcctc gcgccaggcg ggccggacga tccgcggcga ggatctcgtc gacgaggccg
     3181 accggcgcat ccatcacgca cgctggctcg tgcggccggt cgcgacgctc ggacggcgcc
     3241 tgctccgccg cgggctgctc cggccggaga cccgcctcgg gcgcctcgcc gccgagctcc
     3301 acacgccgtt cgacgcggtc gagctcgcga gcgcggcggt cgcgcggggg aacctcaagg
     3361 tcttcgagga gatcgggctc gagttcgcgc gcttcctcgc cgcctgcccg cccgatgcga
     3421 acgacgccgc gctcgagggg ttcctcgccg ggctccgcga gggcgatccg cccgagggcc
     3481 agggctacct gcgccgtgcg ttcacgcgct acgagcgggc gaggcgcacg gacgacccgc
     3541 gcgcggcggc gcagctgacc gtgctcgcga acctcgagat cggctttcac gagcagacgc
     3601 gcctccagcc ggagatcctc gaggcgctcg acgcgccgtt cgtcacgcag gagcggctcg
     3661 gcctgcgcgc gctcgaggcg ctcttcccgt cgctcgcgcg ggcctggccg gtcgtccgcg
     3721 ccccggccgc cgcggtcgcc ggcgcgttcg caaagggcgt gcagcgcgcg tcgagccgcc
     3781 tcgcacggga gctcgtgacc gagagcttca tgacgctcag cctccccggc cgcgtgctcc
     3841 tgctcggccg gcacctcgag gacgcgttcc cggacgcgct cggcgacccg cgcgacgccg
     3901 agctggcgga gctcctcgcc cgcttcgagc ccgccccgcc ggcccgcgac gactgcggcg
     3961 cgcgcgactg ggccgacctg cgccagcgga tgcactacat cgtccacctc ttctgcgcct
     4021 tccacgagcg cgacgacctc ttccgcccgc ccttcacgcc cgcgcaggtc gccgcgttcg
     4081 agcgcggcct cgttcccgac ggggagctgt agcgccgacc ccaggcaaag gagagggcgg
     4141 ccgaagccgc cctctccacg ctcgtccgcg taggcggatt cgctattcca tgtgatccat
     4201 ggtctgcggg ttcaggtggt cgacccgctt cggcttgccg accttctgca tgatcgtgcg
     4261 gtacacgacc atgccctcgg ccagcgggtt gttcgtgacg ccgtccgtcg cgtagacggg
     4321 caggcccggg ccgttcttga acatcgggcc cgtgtacggg taggccgacc aggtgccgga
     4381 gttctgagcc gggtcgtagt acgcgatctc cttcagccgc gtcggcttcg agaagtcgat
     4441 cacgtccatc ccgcccgtgt accaggcgtt cacgaggagg tcacgcttga tgcccatgac
     4501 cgccatcccc atgtgcgcgg agcagtactc gcccggcgcg gggttcggcc gcacgtcgcc
     4561 gacgttgaac tcgctgagga atgcgcccgt ctccatgtcg gagaagaaca tgctccccgt
     4621 cttctgcggg ccggccggat gccacggcct cgcctgccag ttcgtcatcg tcgggcagcc
     4681 gctgccgaac gactcgtcga cccagttcac cacggtcgcg tcgttgttga acatcacgga
     4741 gtggaagaag tccaccgcgc ccgggatgtc gccggtgccg ccggagctga acgcgtcgtc
     4801 gccgatcatg atcgggttca gcgtgtccgg gactccgccg gggccgatgt tccagacctg
     4861 gccctgctcg gcgcaggcgc cgccggccag ccggtgctcg acgtggacga cgatgtcgtg
     4921 gcacgcgatc gaggcgggct cgagaacgcc gggcgcgcag agtccgcgct cgcagtagtc
     4981 gaagatcccg tccgggtcac ccgggtacgc gatcgccggc tgcacgttca tctcggtggc
     5041 cgccgccggg ttgttcagcg gaacctcgac catctggatg acgccgtgca gcgggctgtt
     5101 cgggctgccc ggatccccgt cgtacgggtt ggccaggttg ttgtagcgaa gctggccgca
     5161 cgtcgggccc ggccgcagcg ggtacgacga gacgtagacg acgagcttgt tctgcgtctc
     5221 gacctcgccc ggccagagcg tgatcgtgtg ggcgccgcag tccgtgtaca cggccgcgac
     5281 ctgctggatg ttcgtgaacg gattggccgg gttgtcgctc atcgtgaaga cgcggacgcc
     5341 ctcccagccc atcgggtcgt cgtgcgcgct ccgcggggcg ccgcagttcg gattcgccat
     5401 cgtgcggtcg acggcgagca agagcaggtc ggcaacgccg ttgccgttcc ggtcccagac
     5461 gatcggatcg gcctggagcc cgtcgcaggc gaagtcgcgg atgagccgcg gagcggccgg
     5521 attcttgatg tcgaagatcc ggacgcctcc gcgaggcgcg gcgtcgccgg tgtagtagcc
     5581 gacgaacgcg tggttccccc agaacgcgag gtcggagttc gtcgccgcgt tcggggacgt
     5641 gaagatctcc tgcaccttga agctctggtc gccgacctgg gcgcccgccg tcacggtgag
     5701 cactcccatg gccagcaacg ccaaggtggc caagcgcaag acacgtcgca tggaactccc
     5761 ctttcctgat gtcgtcccga ctggcgcgcg cgatcgtaac ggctcgcacg ttgcctgtcc
     5821 agcgatgcgg agtgggactt acttcacgct tgcgacatag ccgcgcacgt ccgcggccgg
     5881 ctccgtgtac gcggcgccga ggttgtcctc catccgcgcc atgaagtcct ccgtccactg
     5941 cgggaacggg tagtcgatcg cgcgcaggaa ctcgagcgtc gccgcgaggc ggatgtcggc
     6001 gatcgaggga tggtcgccgc cgatgaacgg ctttccgtcg aggaagaacg tgttgaagac
     6061 ctcgagcggc tcggcgaggg cggcctcggc gtccttctgc gcctgtgcct tgagctcgtc
     6121 gctcgcctcg gaggtcgcca cctcgcccgg gtactggccg aacgcgagcg tcgggtacgt
     6181 cgcgcgcgcg aggagcggat agaacgtccc gatgaggtag aacatcgcgt tgttcaccat
     6241 cgcccgccgg ccggggtcgc tcggatagaa gttctcgagc ccgtgcttgt cggcgaggta
     6301 cgccatgatc gcgcagctct cgccgagcgc cccgcgcgga aggtcgggcg actcgaccat
     6361 cggcgtgagg tgggccgggt acttcgccat gtactccggg gacgtcgtcg agccccagac
     6421 gtcctgctcc tcgaagtcga gccccgccgc gcgggcgaac acccggacgg acatgttgtt
     6481 cacgctgggc ttcaggatgt tgagcgtgta ctcggccatg gctcctccta gtaagggttt
     6541 ctcggggacc ggtcgtcgga gagagattcg cgcgagcgcg ccgtgaggat ctcgatcgcg
     6601 tccgccaggt gctcctggtc gatgttgtag gcgccccgct cgacgcggag cctgtgggcc
     6661 tccagcaaca cctccgggtg cgtgaagccg tgcggcggga cgaagcggta cgaggcgagc
     6721 tcgatcagca tgccgagcgg atcctcgaag tagatcgagt ccatgaaccc gcggtctttc
     6781 acgcccgagt gccggatccc acgctcgtcc agccgctcga ccacctgccg gaacgtcgcc
     6841 tgcgagacgt cgaacgcgat gtggtggacg tacccggctt ccttcgcgtc cctgatcgag
     6901 gcgacccgcg
//
LOCUS       DASTFC010000154         5630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_167649,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000154 DASTFC010000000
VERSION     DASTFC010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167649"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1048)
                     /locus_tag="VFO88_04615"
     CDS             complement(71..1048)
                     /locus_tag="VFO88_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9544837.1"
                     /translation="MTRTRVGRRNGLAALQAAVSLVALGAVIWWATRQEAPSFPRTGE
                     AAAALLAALGIYTAATLARGERWYRILEHGGFAAGRGDAYRLTVVGYMGNNVLPARSG
                     DLLRAFLLAPITGRRKRELLGTVVAERLLDAGALVVVFAAVVLGLLNEAEIPGGQTVL
                     LGAGGLALLAALGLVAVVAIRRANALERVRAALRPFAAPSRALASAHGAGLLVLSLAI
                     WALEAGVYVAVGHAAGIDLTPVEALYVVALTNLFALVPAAPGYVGTFDAAVLFAVGSL
                     GERGSDALAYLVLLRFVLFVPITLVGLAFLVTRYGGWSRFRAARLETPG"
     gene            1101..2111
                     /locus_tag="VFO88_04620"
     CDS             1101..2111
                     /locus_tag="VFO88_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9544838.1"
                     /translation="MKLVIQIPCLNEAETLPATLADLPRSMDGFDTVEWLVVDDGSTD
                     GTASVARKHGVDHVVRFTQNKGLAVAFQTGLDAALKLGADVIVNTDADNQYVAADLPL
                     LLGPILAGRADMVVGDRQVRGHEEFSRSKKFLQQWGSWVVRHASDTDLPDTTSGFRAY
                     NREAALRLNVVSDFSYTLETIIQAGKSDIAVTHVPIRTNPRTRDSRLFRSTFQYLRRS
                     VATILRVYTMYEPLRIFLWLAALPGLLGLVLFGRFAWFYLTGPDPTGHIQSLVIGAAL
                     LILSVQLVALGIIADLMRANRVLTERTLHRVRRLELEAGVRPESLAEEAVVDEREPVV
                     PG"
     gene            2114..2740
                     /locus_tag="VFO88_04625"
     CDS             2114..2740
                     /locus_tag="VFO88_04625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9544839.1"
                     /translation="MATDGSLADLQGPAGNTYDKYGTRNPVARVLVGRFLAAVQESVG
                     ELRPDSLLDVGCGEGIVTERLARAFPSTRVVGVDVADPSLLEEWEARRAPNLGFAPGS
                     AYALPYEDDEFEVVTAFEVLEHLERPGEALAELARVAGRALVLSVPREPWWRMTNALS
                     GRYVRDLGNTPGHVNHWSRGAFRRFAGGAGEVRRVRSPYPWTVVTVAR"
     gene            complement(2737..3573)
                     /locus_tag="VFO88_04630"
     CDS             complement(2737..3573)
                     /locus_tag="VFO88_04630"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9544840.1"
                     /translation="MRRRLSALAVASLAAVLAAPTASAAVVDGKRAKKFVYSLSANGH
                     RVAGTPNERRGAWLVGQRFRKFDLPVTIQKFPLPNGRNSRNVIAKTGGPVRVLIVAHM
                     DSVRAGPGANDNGSGVAAMLEVANVLKETEGIWFAALGAEEREMTGSHLHLGSDRLVR
                     SIPRIVRPQIVLALSLDMVGYGPTLNIRGLETSPNRSARRAINRARALGYAPSYLRDS
                     GVSDHAEMTRGGIPAALVTYRWDPCWHLSCDRPARVVPRKMGNAARLTIAAIRKALDA
                     AG"
     gene            complement(3593..4327)
                     /locus_tag="VFO88_04635"
     CDS             complement(3593..4327)
                     /locus_tag="VFO88_04635"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET9544841.1"
                     /translation="MAPSPWSFHLAAGEIAIVAVVAGLALAAARAGRPSRARTGALAA
                     GVALTAGVLLTPVATIALNYLLAAHLFQNVALAEWVPLLLVLGIAPALAERLTANAPA
                     RALTHPFVALPLWLVSYALWHIPAVYDAALRNHALLALEHVSYLVTGLLLWWPVVQDA
                     PHRLSNAARAVYVFAAFLLASPVGLLLTFLPDPIYGFYEEAPRLWGLSPLADQQLAGV
                     IMAGSEAVVFFAAFAFFLSRFLEDER"
     gene            4426..4608
                     /locus_tag="VFO88_04640"
     CDS             4426..4608
                     /locus_tag="VFO88_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544842.1"
                     /translation="MHGLKALSLANAFVPPHELQRHTRAGRRRASWAGRLVRFAARRP
                     APAHPAPRVRRPSPTA"
     gene            4765..4837
                     /locus_tag="VFO88_04645"
     tRNA            4765..4837
                     /locus_tag="VFO88_04645"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4798..4800,aa:Gly,seq:tcc)
     gene            complement(5319..>5630)
                     /locus_tag="VFO88_04650"
     CDS             complement(5319..>5630)
                     /locus_tag="VFO88_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544843.1"
                     /translation="GLLLLVVAFCLAEAAAPAPVAWSPVTAAAAVAVLGATLFASVVE
                     GGTWWFLGPAAVLVAAWAFVRPRPLLAACAAAFVLAALLLAGWAAWHGGVPEFSELEW
                     I"
BASE COUNT          799 a         2034 c         2101 g          696 t
ORIGIN      
        1 gcgcgctcgc gcccggccgg cgcgtcgcgc gtcctctcga gcacgttcgt ggcggccaca
       61 ggaggtgaac ctacccaggc gtttccagcc gcgccgcccg gaagcgcgac cacccgccgt
      121 agcgggtgac gaggaaggcg aggccgacga gggtgatcgg cacgaagagg acgaagcgca
      181 ggaggacgag gtaggcgagc gcgtccgacc cccgctcccc cagcgagccg accgcgaaga
      241 ggacggcggc gtcgaacgtc ccgacgtagc ccggcgccgc ggggacgagc gcgaagaggt
      301 tcgtgagcgc gacgacgtag agcgcctcca cgggcgtgag gtcgatcccc gccgcgtggc
      361 cgacggcgac gtagacgccg gcctcgagcg cccagatcgc gagcgagagg acgagcagcc
      421 ccgccccgtg ggcgctcgcg agcgcgcggc tcggggcggc gaacgggcgc agggccgccc
      481 gcacccgctc gagcgcgttc gcccggcgga tcgcgacgac cgcgacgagc ccgagcgccg
      541 cgagcagcgc gagcccaccc gcccccagga gcaccgtctg cccgcccggg atctcggcct
      601 cgttcaggag gccgaggacg accgcggcga agacgacgac gagcgcgccg gcgtcgagca
      661 ggcgctccgc gacgacggtc ccgaggagct cccgcttgcg ccgccccgtg atcggcgcga
      721 ggagaaaggc gcgcaggagg tcgccgcttc gcgcggggag gacgttgttc cccatgtagc
      781 cgaccaccgt cagccggtac gcgtcgccgc gcccggccgc gaagccgccg tgctcgagga
      841 tccggtacca ccgctccccg cgcgcgagcg tcgccgccgt gtagatcccg agcgcggcga
      901 ggagcgcggc ggccgcctcg ccggtgcgcg ggaagctcgg cgcctcctgc cgggtcgccc
      961 accagatcac ggctccgagg gcgacgagcg agaccgccgc ctgcagcgcg gcgagcccgt
     1021 tccgccgccc gacccgtgtt cgcgtcacga gggaatcctg cccgtcgtgg cccgcccgcg
     1081 cgggttaccc tctctccagg gtgaagctcg tcatccagat cccgtgcctg aacgaggcgg
     1141 agacgctccc cgcgacgctc gccgacctgc cgcgctccat ggacgggttc gacacggtcg
     1201 agtggctcgt cgtcgacgac ggctcgacgg acgggacggc ctcggtcgcg cggaagcacg
     1261 gcgtcgacca cgtcgtccgc ttcacgcaga acaaggggct cgcggtcgcg ttccagacgg
     1321 ggctcgacgc ggcgctcaag ctcggcgcgg acgtgatcgt gaacacggac gccgacaacc
     1381 agtacgtcgc cgccgacctg ccgctcctgc tcggcccgat cctcgccggg cgggccgaca
     1441 tggtcgtcgg cgaccggcag gtgcgcggcc acgaggagtt ctcccgctcg aagaagttcc
     1501 tccagcagtg gggctcgtgg gtcgtccggc acgcgtcgga cacggacctg cccgacacga
     1561 cctccggctt ccgcgcctac aaccgcgagg cggcgctccg gttgaacgtc gtctcggact
     1621 tctcgtacac gctcgagacg atcatccagg cgggaaagag cgacatcgcg gtcacgcacg
     1681 tccccatccg gacgaacccg cggacgcgcg actcgcggct cttccgctcg accttccagt
     1741 acctgcgccg ctcggtcgcc acgatcctgc gcgtctacac gatgtacgag ccgctccgga
     1801 tcttcctctg gctcgccgcg ctgcccgggc tgctcgggct cgtcctcttc gggcgcttcg
     1861 cgtggttcta cctcaccggc ccggatccga ccggccacat ccagtccctc gtgatcggcg
     1921 cggcactgct catcctctcc gtccagctcg tcgcgctcgg gatcatcgcc gacctcatgc
     1981 gcgcgaaccg cgtcctcacc gagcggacgc tccatcgcgt gcgccggctc gagctcgagg
     2041 cgggcgtgcg gcccgagtcg ctcgcggagg aggcggtcgt cgacgagcgc gagccggtcg
     2101 tccccgggtg acgatggcga ccgacggctc gctcgcggat ctccagggcc cggcggggaa
     2161 cacgtacgac aagtacggga cgcggaaccc ggtcgcgcgc gtcctcgtcg gccggttcct
     2221 cgcggccgtg caggagtcgg tgggcgagct gcgccccgac tcgctcctcg acgtcggctg
     2281 cggcgagggg atcgtgacgg agcgccttgc gcgcgccttc ccgtcgacgc gggtcgtggg
     2341 cgtcgacgtc gccgacccct cgctcctcga ggagtgggag gcgcggcggg cgccgaacct
     2401 cggcttcgcc ccgggctccg cgtacgcgct cccgtacgag gacgacgagt tcgaggtcgt
     2461 gaccgcgttc gaggtgctcg agcacctgga gcggccgggc gaggcgctcg cggagctcgc
     2521 ccgcgtcgcc ggccgcgcgc tcgtcctgag cgtcccgcgg gagccgtggt ggcggatgac
     2581 gaacgcgctc tccggccggt acgtccgcga cctcgggaac acgccgggcc acgtgaacca
     2641 ctggtcgcgc ggcgccttcc gccggttcgc gggcggcgcg ggcgaggtgc gccgcgtccg
     2701 ctcaccgtac ccgtggaccg tggtgaccgt cgcgcgctag ccggcggcgt cgagcgcctt
     2761 gcggatcgcg gcgatcgtga gccgcgccgc gttccccatc ttccgcggca cgacgcgggc
     2821 cgggcggtcg caggacaggt gccagcacgg gtcccaccgg tacgtgacga gcgccgccgg
     2881 gatgccgccg cgggtcatct ccgcgtggtc cgagacgccg gagtcccgca ggtagctcgg
     2941 cgcgtagccg agcgcccgcg cgcggtttat cgcacggcgc gcggagcggt tcggcgacgt
     3001 ctcgagcccc cggatgttga gcgtcggccc gtagccgacc atgtcgagcg acagcgcgag
     3061 gacgatctgc ggccggacga tccgcgggat gctcctgacg agccggtcgg agcccaggtg
     3121 gaggtgcgag ccggtcatct cccgctcctc cgcgccgagc gccgcgaacc agatcccctc
     3181 cgtctccttc agcacgttcg cgacctcgag catcgcggcg acgccggagc cgttgtcgtt
     3241 cgcgcccggc cccgcgcgga cggagtccat gtgcgcgacg atgagcacgc gcacgggccc
     3301 gccggtcttc gcgatcacgt tgcgcgagtt gcgcccgttc gggagcggga acttctggat
     3361 cgtcacgggc aggtcgaact tccggaagcg ctgcccgacg agccaggcgc cgcggcgctc
     3421 gttcggcgtc cccgcgacgc ggtggccgtt cgcggagagc gagtagacga acttcttcgc
     3481 ccgctttccg tccacgacgg ccgcggaggc ggtcggcgcg gcgaggacgg cggcgaggct
     3541 cgcgacggcg agcgcggaaa ggcggcggcg cacgaccgtc agggtagagc agtcaccgct
     3601 cgtcctcgag gaatcttgag agaaagaatg caaaggcggc gaagaagacg accgcctcgg
     3661 agccggccat gatcacgccc gcgagctgct ggtcggcgag cggcgagagc ccccagagcc
     3721 gcggcgcctc ctcgtagaag ccgtagatcg ggtccgggag gaaggtgagc aggagcccca
     3781 ccgggctcgc gagcaggaat gcggcgaaga cgtacaccgc gcgcgcggcg ttcgagagcc
     3841 ggtgcggagc gtcctggacg acgggccacc agaggaggag cccggtcacg aggtacgaca
     3901 cgtgctcgag tgcgagcagc gcgtggttgc ggagcgcggc gtcgtacacg gccgggatgt
     3961 gccagagcgc gtacgagacg agccagagcg ggagcgcgac gaacgggtgc gtgagcgcgc
     4021 gcgcaggcgc gttcgccgtg aggcgctcgg cgagcgccgg cgcgatcccg agcacgagga
     4081 gcagcggcac ccactccgcg agcgcgacgt tctggaagag gtgtgcggcc aggaggtagt
     4141 tgagtgcgat cgtcgcgacg ggcgtgagga gcacgccggc cgtgagggcg acgccggcgg
     4201 cgagcgcgcc cgtgcgggcg cgggacggac ggccggcgcg cgcggcggcg agcgcgagcc
     4261 cggcgacgac ggcgacgatg gcgatctccc cggcggccag gtggaagctc cagggggagg
     4321 gcgccatcgc ggcaccgtac gccacgcaaa gactgtcccg gggggctagt gcggttcatc
     4381 acggggaggt ctagaacggc tccaagcaac cgaaggagcg atccgatgca cggtctcaaa
     4441 gcgctcagcc tggccaacgc cttcgtcccg ccgcacgagc tccagcgcca cacgcgcgcc
     4501 ggcaggcggc gtgcctcgtg ggcgggccgc ctcgtccggt tcgccgcgcg ccgcccggcg
     4561 ccggcccacc cggccccgcg cgtccgccgc ccgtcgccga ccgcgtagga gctacgatcg
     4621 agctacgcgg gttcgccggc ctgaagtccg cccgcttcgc gggcatgacg acttcggccg
     4681 ggtgagggcg atcgctcgcc cgtcgcgtgg ccgcgcccgc cgtgtcgggc gcggcgacgg
     4741 aggtccgcgc tacgatcggc gcacgcgggt gtagctcagt tggtagagca tcagccttcc
     4801 aagctgaggg tcgcgggttc gagtcccgtc gcccgcttca ccgggaaccc gctggaaacg
     4861 gcgggttttc ttgtgggcga gcctcgagcc cgccgcgtcg aatcgggacg ctagtcagcc
     4921 tttagtgagc tttcgccgcg ttccaggcgt gcccgcagct cacctatcgt ccggttcgcc
     4981 ggttcgagcc ggtttagtag ctcgcgctcc gcgggcgaga cagcccggac tagccctgcc
     5041 tcttgcagcg cgaacgcggg atcaagcggc gacggccgcg gagaacggaa cgcaacgtgc
     5101 cgcggcccaa ccggcctgca acgggtaacg aaggggtgtc agtgggggtt gggtgcgggg
     5161 tgacaacggg tgacattcgc gctcgccact tgcggcggcc cgcacgtctt gacagtgcgg
     5221 ctcatgcggg attgggcctc gcattttccg agcgggacgg tccgccacgg cgtcccgcgt
     5281 cggcggccct atcgtcgtgc ccggcaggct gcggcaggct agatccactc gagctcgctg
     5341 aactccggca cgccgccgtg ccaggcggcc cagccggcga ggaggagcgc tgcgaggacg
     5401 aacgccgcgg cgcaggcggc gagtagcggg cgcggccgga cgaatgccca ggctgcgacg
     5461 agcaccgccg cgggtccgag gaaccaccac gtcccaccct cgaccacgga cgcgaagagc
     5521 gtcgcgccga gaacggccac cgcagcggcc gccgtgaccg ggctccacgc gaccggcgcg
     5581 ggcgccgccg cctcggcgag gcagaacgcg acgacaagga gcaggagtcc
//
LOCUS       DASTFC010000155         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_794033,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000155 DASTFC010000000
VERSION     DASTFC010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794033"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFO88_04655"
     CDS             <1..906
                     /locus_tag="VFO88_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET9544844.1"
                     /translation="HLLARCGDEQSVRLPASELEERFHLDREQLTEHLALLNLVNFGG
                     GCYAVYANLDGDEVHVQKELFGDAFRRAPRLTPLEARAIRLALEFVGPMIAAEAHTPL
                     ERVRRKLEETFGQFEQASEDEDAEPTAEESLVGTLSQAIRESRVVEIEYKAVGEETST
                     RTIEPHDLERELPHWYVHSWDRTRDAQRSFRLDRMKSVTLLDETFEPRREVVPQKLRD
                     VQTARVLFRKEAAAWRIERGAVPLSDGSALEEVRHGSADWLVGEILSYRGAAVVLEPA
                     EIREAVATRAKKLAKELRRRPAARR"
     gene            complement(903..2708)
                     /locus_tag="VFO88_04660"
     CDS             complement(903..2708)
                     /locus_tag="VFO88_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9544845.1"
                     /translation="MTRAAGYERRREQWIAWVRLLALGWAVAEVSYVAHDYPPGHHTA
                     AWIATGVLAVGALTLFGLARREWSDRGRTILGAAALAFDVAIIYAFVATFVFEAGSPA
                     SRLVVLVVIEAAVRYGAAGGLILTALNAPILLVVEMWRSENFAPETFQLRNVTLALGI
                     QLVAGLIVGGLVRQLREEAGEAEERAAEAETLRDEIGRHADQLEVVNRAARALSSSLD
                     LDESWERFVREVRAGLPFDRIALLLADGPRAIVIADAGLGEEGAGGSDWSRPVDETAL
                     AEVLREGTTVVSPDIGAAPRYPEEAEIAALGLRSRVVAPLAVAGRALGGLSVSRLDPD
                     AFTREEVDLVTLLGRQVGTAVENIRAFEAERAAAEELRRLSALRADFVSLVSHELRGP
                     MASVIGCASTLRQRWRTLAPEQRESFLALIDEETTRLAELIGDVLDTSRLEAGTFTYA
                     FTDVDVGELVRDVAAVVDLGQEEVAVSASVTGSLPPVRGDRERLRQLVMNLLTNAVKY
                     TVAGDEIDVRAYADDGEVLVSVSDNGPGISPEEQRLIFEKFGRAAAASGSKPGAGLGL
                     FIARSIAEAHGGTLGVTSERGRGATFTVRLPAERV"
     gene            complement(2705..3727)
                     /gene="queG"
                     /locus_tag="VFO88_04665"
     CDS             complement(2705..3727)
                     /gene="queG"
                     /locus_tag="VFO88_04665"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9544846.1"
                     /translation="MTTTTRISQAELARLGDELGLDALGAARPEPYADTERHIAARKA
                     RGLFADMRFTMAQPETSCHPESLLPGARSVVSAALSYYAPGPEPRADEGRLPRYTWSD
                     RYAQLSEKLDELGRRLGGAYRVLVDANAHVDREGAARAGVGFYGKNTLLITRRHGSWV
                     VLGTLVTEADVEPSAPLALDCGSCRLCVDACPTGALDEPGTLDATRCLSYWTQSPQPI
                     PEAYREPLADQVYGCDICQDVCPWNRGTERRHAGDPLPDGAEPTVSLADWLTASEDEL
                     AARYDRLYVPRNDGRYLRRNALVAAGNSGDPSLLPLVERHAESGDELVRGHARWAAAR
                     LAGDAA"
     gene            3766..>4472
                     /locus_tag="VFO88_04670"
     CDS             3766..>4472
                     /locus_tag="VFO88_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9544847.1"
                     /translation="MKAVRIHEDGGPEVLRYEEAPDPSPGPGEVLLSLRAASLNHLDV
                     WLRRGLPSVPKPRILGADGAGVVASLGEGVRGVEVGDAVVLNPGLDDGKHIVGEHMDG
                     THAELIAVPAEYVHPLPDGMSFEEAAAFPLVFATAYRMLATRAKVREGEWVLVWGIGA
                     GVATASLAIAKALGARVLATSTSDEKLVRAKELGADEVVRTDEDVVAAARELTDGAGV
                     DIVVEHVGEATWKSSLQA"
BASE COUNT          593 a         1612 c         1714 g          553 t
ORIGIN      
        1 cacctgctcg cgcgctgcgg ggacgagcag agcgtccgcc tgccggcgtc cgagctcgag
       61 gagcggttcc acctcgaccg cgagcagctg accgagcacc tggccctcct gaacctcgtg
      121 aacttcggcg gcgggtgcta cgccgtctac gcgaacctcg acggcgacga ggtgcacgtc
      181 cagaaggagc tcttcgggga cgccttccgc cgcgcgccgc gcctgacccc gctcgaggcg
      241 cgcgcgatcc gcctggcgct cgagttcgtg gggccgatga tcgcggccga ggcgcacacg
      301 ccgctcgagc gcgtccgccg caagctcgag gagacgttcg gccagttcga gcaggcctcc
      361 gaggacgagg acgccgagcc gaccgccgag gagagcctcg tcggcacgct ctcgcaggcg
      421 atccgcgagt cgcgcgtggt cgagatcgag tacaaggcgg tcggcgagga gacgagcacg
      481 cggacgatcg agccgcacga cctcgagcgc gagctcccgc actggtacgt gcacagctgg
      541 gaccggacgc gggacgcgca gcggagcttc cggctcgacc ggatgaagag cgtgacgctc
      601 ctcgacgaga cgttcgagcc gcgacgcgag gtcgtcccgc agaagctccg cgacgtccag
      661 acggcgcgcg tgctcttccg caaggaggcg gcggcatggc ggatcgagcg cggggcggtg
      721 ccgctctcgg acggctcggc gctcgaggag gtgcgccacg gcagcgcgga ctggctcgtc
      781 ggcgagatcc tgtcctaccg tggagcggcg gtcgtgctcg agccggcgga gatccgcgag
      841 gcggtcgcga cgcgggcgaa gaagctcgcg aaggagctcc gccgccggcc cgcagcgcgc
      901 cgctagacgc gctcggccgg caggcgcacg gtgaacgtcg cgccccggcc gcgctcggac
      961 gtgacgccga gcgtgcctcc gtgcgcctcg gcgatcgagc gggcgatgaa gagcccgagc
     1021 cccgcgcccg gcttcgaccc gctcgccgcc gcggcgcgcc cgaacttctc gaagatgagg
     1081 cgttgctcct cgggcgagat gcccggcccg ttgtcgctca cgctcacgag cacctcgccg
     1141 tcgtcggcgt aggcgcggac gtcgatctcg tcgcccgcga cggtgtactt gacggcgttc
     1201 gtgaggaggt tcatgacgag ctggcgcagc cgctcgcggt cgccgcggac cggcgggagc
     1261 gagcccgtca cgctcgcgct gacggcgacc tcctcctgcc ccaggtcgac gaccgccgcg
     1321 acgtcgcgga cgagctcgcc gacgtccacg tccgtgaagg cgtacgtgaa cgtcccggcc
     1381 tcgagccgcg acgtgtcgag gacgtcgccg atcagctcgg cgagccgggt cgtctcctcg
     1441 tcgatcaggg cgaggaagct ctcccgctgc tcgggcgcga gcgtccgcca gcgctgccgg
     1501 agcgtcgacg cgcatccgat cacggaggcc atcgggccgc ggagctcgtg cgagacgagc
     1561 gagacgaagt cggcgcggag cgcggagagg cggcggagct cctccgcggc cgcgcgctcc
     1621 gcctcgaacg cgcggatgtt ctcgaccgcg gtgcccacct ggcgcccgag gagcgtcacg
     1681 aggtcgacct cctcgcgcgt gaacgcgtcc gggtcgaggc gcgagaccga gaggccgccg
     1741 agggcgcgac cggcgacggc gagcggcgcg acgacgcgcg agcggagccc gagcgccgcg
     1801 atctccgcct cctccggata gcgcggggcg gcgccgatgt ccgggctcac gaccgtcgtc
     1861 ccctcgcgga gcacctcggc gagcgccgtc tcgtcgaccg gccgcgacca gtcggagccg
     1921 ccggcgcctt cctccccgag cccggcgtcc gcgatcacga tcgccctcgg cccgtccgcg
     1981 agaaggagcg cgatccggtc gaacgggagc ccggcgcgca cctcgcggac gaaccgctcc
     2041 cagctctcgt ccaggtcgag cgaggacgag agcgcgcggg ccgcgcggtt cacgacctcg
     2101 agctggtcgg cgtggcggcc gatctcgtcg cggagcgtct cggcctcggc tgcacgctcc
     2161 tcggcctcgc ccgcctcctc ccggagctgg cgcacgaggc cgccgacgat caggcccgcg
     2221 acgagctgga tcccgagcgc gagcgtcacg ttcctgagct ggaacgtctc cggggcgaag
     2281 ttctccgagc gccacatctc gacgacgagg aggatcggcg cgttcaaggc ggtgaggatg
     2341 aggccgccgg ccgcgccgta ccggacggcg gcctcgatca cgacgaggac gacgagcctg
     2401 ctcgccggcg agccggcctc gaacacgaag gtggcgacga acgcgtagat gatcgcgacg
     2461 tcgaacgcga gggcggcggc gccgaggatc gtgcggcccc ggtcgctcca ttcccggcgg
     2521 gcgagcccga agagcgtcag cgcgccgacg gcgagcacgc ccgtcgcgat ccacgcggcc
     2581 gtgtgatggc cgggcggata gtcgtgggcg acgtaggaga cctccgcgac ggcccagccg
     2641 agcgcgagga gccgcaccca ggcgatccac tgctcccggc ggcgctcgta gccggcggcg
     2701 cgggtcatgc cgcgtctccg gcgaggcggg ctgcggccca gcgggcgtgt ccgcggacga
     2761 gctcgtcgcc gctctcggcg tggcgctcga cgagcgggag caggctcgga tcgcccgagt
     2821 tccccgcggc gacgagcgcg ttccggcgca ggtagcgccc gtcgttccgg gggacgtaca
     2881 ggcggtcgta gcgcgccgcg agctcgtcct cggacgccgt cagccagtcg gcgagcgaga
     2941 cggtcggctc ggcgccgtcc gggagcgggt cgccggcgtg gcggcgctcg gtgccgcggt
     3001 tccaggggca gacgtcctgg cagatgtcgc agccgtagac ctggtcggcg agcggctcgc
     3061 ggtacgcctc cgggatcggc tgcggcgact gcgtccagta cgacaggcag cgcgtcgcgt
     3121 cgagcgtgcc gggctcgtcg agcgcgccgg tcgggcaggc gtccacgcag agccggcacg
     3181 agccgcagtc gagcgcgagc ggcgcgctcg gctcgacatc cgcctcggtc acgagcgtcc
     3241 ccaggacgac ccaggagccg tgccgccgcg tgatgaggag cgtgttcttg ccgtagaacc
     3301 cgacgccggc gcgtgcggcg ccctcgcggt cgacgtgcgc gttcgcgtcg acgagcacgc
     3361 ggtacgcgcc gccgaggcgc cgtccgagct cgtcgagctt ctcgctcagc tgggcgtacc
     3421 ggtcgctcca cgtgtagcgc ggcagccggc cctcgtccgc gcgcggctcc gggcccggcg
     3481 cgtagtacga gagcgccgcg gagacgacgc tgcgcgcgcc ggggagcagc gactcgggat
     3541 ggcaggacgt ctcgggctgc gccatcgtga agcgcatgtc ggcgaagagc ccgcgcgcct
     3601 tgcgcgcggc gatgtggcgc tcggtgtccg cgtagggctc ggggcgtgcc gcgccgagcg
     3661 cgtcgagccc gagctcgtcc ccgagccgcg cgagctccgc ctggctgatc ctcgtcgtcg
     3721 tcgtcatgcc cccttcaaca ctctaggcgc gtagagtccc catccgtgaa agcggtacgc
     3781 atccacgagg acggcgggcc cgaggtcctc cgctacgagg aggcgcccga cccgtcgccc
     3841 ggccccggcg aggtgctcct gtccctccgg gcggcgtcgc tcaaccacct ggacgtgtgg
     3901 ctccggcgcg gccttccgtc ggtgccgaag ccgcgcatcc tcggggcgga cggcgccggg
     3961 gtcgtcgcgt cgctcggcga gggcgtgcgc ggcgtcgagg tcggcgacgc ggtcgtcctc
     4021 aacccgggcc tcgacgacgg gaagcacatc gtcggcgagc acatggacgg cacgcacgcg
     4081 gagctgatcg cggtgcctgc ggagtacgtc cacccgctcc ccgacggcat gtccttcgag
     4141 gaggcggcgg ccttcccgct cgtgttcgcg acggcgtacc ggatgctcgc cacgcgcgcg
     4201 aaggtgcggg agggcgagtg ggtgctcgtg tggggcatcg gcgccggggt cgcgactgcg
     4261 tcgctcgcga tcgcgaaggc gctcggggcg cgcgtgctcg caacgtcgac gagcgacgag
     4321 aagctcgtcc gggcgaagga gctcggcgcg gacgaggtcg tgcggacgga cgaggacgtc
     4381 gtcgcggccg cgcgcgagct gacggacggc gccggcgtgg acatcgtcgt cgagcacgtc
     4441 ggcgaggcga cctggaagtc gtcgcttcag gc
//
LOCUS       DASTFC010000156         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_645583,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000156 DASTFC010000000
VERSION     DASTFC010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645583"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="hemB"
                     /locus_tag="VFO88_04675"
     CDS             <1..712
                     /gene="hemB"
                     /locus_tag="VFO88_04675"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET9544848.1"
                     /translation="AGSGAYDADGIVQRALRAAREAAPGLVLMTDVCLCEYTDHGHCG
                     LVEDGEVVNDASLELLARTAVSHADAGADAVCPSDMLDGRVGAIRAALDDAGYERTPI
                     VAYSAKYASAFYGPFREVADSAPAFGDRRSYQMDPPNVREALRECELDLEEGADAIMV
                     KPALPYLDVVRAARERFDAPLLAYNVSGEYAMVKAAAAQGWLDERAAALESLVAIKRA
                     GADAIVTYWAADAAEWLA"
     gene            709..1251
                     /locus_tag="VFO88_04680"
     CDS             709..1251
                     /locus_tag="VFO88_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544849.1"
                     /translation="MTLWRTIAEDAAAESELWASALLPDAERDEIPVFSPLAPDELAV
                     GLETIYEGYLMHYGRPRLFASGDRDTRILLGDYLYAHGLVRVAEHGDTATVSDLAELL
                     SLCAQLRAEASAGEPIADGVLWAATHALIGSGNGELASAREVLRDRGDARPLAALAAR
                     VAGEDALAQAGRLHAERASL"
     gene            1264..1452
                     /locus_tag="VFO88_04685"
     CDS             1264..1452
                     /locus_tag="VFO88_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544850.1"
                     /translation="MDLLLALVPLAQESHDPAEEGARIIIGMLLVALTFLSVIALGQL
                     ARHLSHRRQARRTERRPY"
     gene            1655..>2299
                     /locus_tag="VFO88_04690"
     CDS             1655..>2299
                     /locus_tag="VFO88_04690"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HET9544851.1"
                     /translation="MPPGDLRSWLDLLRREGELVEVDAEVDPNLEVTEIVDRTVKAGG
                     PALLFTNPKGSDRPLLVNQFGTERRMALGFGVERLDDLGARVADVLELQPPQGLVEKV
                     KGLGKLKKLADSMPKTVSRGACQELVLEPNLDLLPIQHCWPGDPAPFITLPAVITRDP
                     RTGGRNVGMYRMQKIDAKTTFMHWQIHKDGAADWRGMGDRLEVAVALGCDPITTY"
BASE COUNT          298 a          861 c          837 g          303 t
ORIGIN      
        1 cgccggctcg ggcgcctacg acgccgacgg gatcgtccag cgcgcgctcc gcgcggcgcg
       61 cgaggcggcg ccggggctcg tgctcatgac ggacgtctgc ctctgcgagt acacggacca
      121 cggccactgc gggctcgtcg aggacggcga ggtcgtgaac gacgcctccc tcgagctgct
      181 cgcgcgcacg gcggtctcgc acgccgacgc gggcgcggac gcggtctgcc cgagcgacat
      241 gctcgacggg cgggtcggcg cgatccgcgc ggcgctcgac gacgccggct acgagcggac
      301 gcccatcgtc gcgtactccg cgaagtacgc ctcggcgttc tacgggccgt tccgcgaggt
      361 ggccgactcg gcgcccgcgt tcggcgaccg gcgctcgtac cagatggacc cgccgaacgt
      421 ccgcgaggcg ctgcgcgagt gcgagctcga cctcgaggag ggcgcggacg cgatcatggt
      481 gaagcccgcg ctcccgtatc tcgacgtcgt ccgcgcggcg cgcgagcgct tcgacgcccc
      541 gcttctcgcc tacaacgtga gcggggagta cgcgatggtc aaggcggccg cggcgcaggg
      601 ctggctcgac gagcgtgccg ccgcgctcga gtcgctcgtc gcgatcaagc gcgcgggggc
      661 cgacgcgatc gtgacgtact gggccgccga cgcggccgag tggctcgcgt gacgctctgg
      721 cggacgatcg ccgaggacgc ggcggccgag agcgagctgt gggcgtcggc gctcctcccg
      781 gacgcggagc gcgacgagat cccggtcttc tcgccgctcg ctccagacga gctcgcggtc
      841 gggctcgaga cgatctacga gggctacctc atgcactacg gccggccgcg gctcttcgcg
      901 tcgggcgacc gcgacacgcg gatcctgctc ggggactacc tctacgcgca cgggctcgtg
      961 cgcgtggccg agcacggcga cacggcgacg gtgtccgacc tcgccgagct gctctcgctc
     1021 tgcgcgcagc tccgcgcgga ggcgtccgcc ggggagccga tcgccgacgg cgtcctctgg
     1081 gccgccacgc acgcgctgat cggctccggg aacggcgagc tcgcctcggc gcgcgaggtg
     1141 ctccgcgatc gcggcgacgc ccggccgctc gccgcgctcg ccgcccgcgt cgcgggcgag
     1201 gacgcgctcg cgcaggcggg acgcctccac gccgagcggg cgagcctcta gaatcccgcc
     1261 ccgatggatc tgctcctcgc tctcgtcccg ctcgcccagg agagccatga cccggccgag
     1321 gagggcgcgc ggatcatcat cgggatgctg ctcgtcgcgc tcacgttcct ctccgtgatc
     1381 gcgctcggcc agctcgcgcg gcacctctcg caccggcgcc aggcgcggcg caccgagcgc
     1441 cggccctact gaggcgtcct ggtcacgctc gcgatcggca cggccctcca ggccgccggt
     1501 ctcgtcctcg tcccggtcgg ggtcaggtct tgcgttgacg catccggcgc acgatgcaag
     1561 acctgacccc ggggttcgcg cgccgcttcg ccgtcgcgcg gcctgcgcgc tgaccctcgg
     1621 gacgcccgca gcggccggat acactcgaag cacggtgcct cccggcgacc ttcgctcctg
     1681 gctcgacctg ctgcggcggg agggcgagct cgtggaggtg gacgccgagg tcgacccgaa
     1741 tctcgaggtc acggagatcg tcgaccggac ggtgaaggcc ggcggcccgg cgctcctctt
     1801 cacgaacccg aagggctccg accggccgct cctcgtgaac cagttcggaa cggagcggcg
     1861 catggcgctc gggttcggcg tcgagcgcct cgacgacctc ggcgcgcgcg tggcggacgt
     1921 cctcgagctc cagccgccgc agggcctcgt ggagaaggtc aagggcctcg gcaagctgaa
     1981 gaagctcgcg gactcgatgc cgaagacggt ctcgcgcggc gcgtgccagg agctcgtgct
     2041 cgagccgaac ctcgacctcc tcccgatcca gcactgctgg cccggcgacc cggcgccgtt
     2101 catcacgctc cccgccgtca tcacgcgcga cccgcgcacc ggcgggcgga acgtcggcat
     2161 gtaccggatg cagaagatcg acgcgaagac gaccttcatg cactggcaga tccacaagga
     2221 cggcgcggcg gactggcgcg ggatgggcga ccggctcgag gtcgcggtcg cgctcggctg
     2281 cgacccgatc acgacgtac
//
LOCUS       DASTFC010000157         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_991816,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000157 DASTFC010000000
VERSION     DASTFC010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991816"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..634
                     /locus_tag="VFO88_04695"
     CDS             47..634
                     /locus_tag="VFO88_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET9544852.1"
                     /translation="MANELQGKRIAFIAADGVEQVELTEPWKAVKEAGAEVELISIAD
                     GEIQGMNHMDKGDTFKVDKHVSKADPTDYDGLVIPGGVANPDFLRMDEDAVRFTRTFF
                     EQGKPVGAICHGPWLLVEAGVLEGRTVTSWPSLRTDIENAGGSWVDTELQVDRGLVTS
                     RKPDDLPAFSSKLVEEFGEGIHEEQAQAAERAVRA"
     gene            complement(665..1828)
                     /locus_tag="VFO88_04700"
     CDS             complement(665..1828)
                     /locus_tag="VFO88_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9544853.1"
                     /translation="MAALSGGHLATDLAQGALPALLPFLVLELDLSYTMAAALVLAGT
                     FASSIVQPAFGVWSDARGALWLLPAGVAIAGLGMAAASVAPSYPLVVTAVLVSGLGVA
                     AYHPEGSKFASYVSGRRRASGMAFFSVGGNIGFALGPLVASFAVITLGLGLEGGLILA
                     VPGLVAAALLFAARDYLAGFAPSAEQHAARREAPGRPGGLALLLAVVGLRSVAHMGLF
                     TFVPLYEISRGNDAGYGIRLLSGFLLAGAVGTLFGGPLADRFGRRRVLAGSFVVSTPL
                     ILVYALVGGWVGAVALVLAGAAVIGTFGVSLVMSQEYLPARVGLASGLSIGLAIGLGG
                     VAALSLGAVADAIDVHAAVVATAAGPAAALLLTLALPPAPRETRPGAPAAAPV"
     gene            complement(1961..>2919)
                     /locus_tag="VFO88_04705"
     CDS             complement(1961..>2919)
                     /locus_tag="VFO88_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9544854.1"
                     /translation="GLCDLQVNGGAGANVTDGAAALDAIDALQLDHGVTSYLPTVIST
                     APEVAEEAVRELAERADDPRSPVAGIHLEGPFLNPSHRGVHPAERLAAPADGEPPYYA
                     SPAIRLVTLAPELPGALDLVAALRRRGIAVAIGHTGGSAEDAARAAAAGASLVTHLFN
                     GMKDFHHRSPNVPGWALAASRMRVSVIADGLHVDPVALRLVDRLARRRVVLITDASPA
                     AGAADGSYRMAGVEIRAQGGRVTDERGVLAGSALTLDEAVRRWSAFTGAPLAEALAAA
                     SERPARLVGLPGGLEPGAPADLVLLREDGTVERVLRRGAWVR"
BASE COUNT          435 a         1078 c         1137 g          269 t
ORIGIN      
        1 tcgggccccg gtacgcagaa cggacgggac aacgaaagga gaccggatgg cgaacgagct
       61 ccagggcaag cgaatcgcgt tcatcgccgc cgacggcgtc gagcaggtcg agctgaccga
      121 gccgtggaag gccgtgaagg aggccggcgc ggaggtcgag ctgatctcga tcgccgacgg
      181 cgagatccag ggcatgaacc acatggacaa gggcgacacg ttcaaggtcg acaagcacgt
      241 gtccaaggcg gacccgaccg actacgacgg gctagtgatc ccgggcgggg tcgcgaaccc
      301 ggacttcctg cgcatggacg aggacgccgt gcggttcacg cggacgttct tcgagcaggg
      361 gaagccggtc ggcgcgatct gccacgggcc gtggctgctc gtcgaggcgg gcgtgctcga
      421 gggccgcacg gtcacctcgt ggccgtcgct tcgcaccgac atcgagaacg ccggcggcag
      481 ctgggtcgac accgagctcc aggtcgaccg tgggctcgtg acgagccgca agccggacga
      541 cctccccgcg ttctcgtcga agctcgtcga ggagttcggc gaggggatcc acgaggagca
      601 ggcgcaggcg gccgagcggg cggtccgcgc gtagcgaacg cacgccccgc gccggccgcg
      661 cgtcctagac gggcgccgcg gccggcgcgc cgggcctcgt ctcgcgcggc gcgggcggca
      721 gcgcgagcgt gaggagaagc gcggccgccg gccccgccgc ggtcgcgacg acggccgcgt
      781 ggacgtcgat cgcgtctgcg accgcgccga gcgagagggc ggcgaccccg ccgagcccga
      841 tcgcgagccc gatcgagagc cccgacgcga gcccgacccg cgcgggcagg tactcctggc
      901 tcatcacgag gctcacgccg aacgtcccga tgacggccgc ccccgcgagg acgagcgcga
      961 ccgcgcccac ccaaccgccg acgagcgcgt acacgaggat gagcggcgtc gagacgacga
     1021 agctccccgc gagcacgcgc cgccgcccga accggtcggc gagcggcccg ccgaagagcg
     1081 ttccgacggc gcccgcgagc aggaagccgg agagcagccg gatcccgtag ccggcgtcgt
     1141 tcccgcgcga gatctcgtag agcggcacga acgtgaagag gcccatgtgc gcgaccgagc
     1201 ggaggccgac gacggcgagg aggagcgcga gcccgcccgg ccgccccggc gcctcccgcc
     1261 gcgcggcgtg ctgctcggcg ctcggcgcga atccggccag gtagtcgcgc gccgcgaaga
     1321 ggagcgccgc cgcgacgagg cccggcaccg cgaggatcag cccgccctcg agcccgagcc
     1381 cgagcgtgat gaccgcgaac gaggcgacga gcgggccgag cgcgaacccg atgttgcccc
     1441 cgacggagaa gaacgccatc ccgctcgcgc gccggcggcc gctcacgtag ctcgcgaact
     1501 tcgacccctc cgggtggtac gcggccacgc cgagcccgga cacgagcacg gccgtgacga
     1561 cgagcggata cgacggcgcg acggaggccg ccgccatccc gagcccggcg atcgcgaccc
     1621 cggccggaag cagccagagc gcgccgcggg cgtccgacca gaccccgaac gccggctgga
     1681 cgatcgacga cgcgaacgtc cccgcgagca cgagcgccgc ggccatcgtg tacgagaggt
     1741 cgagctcgag cacgaggaac ggcaggagcg cgggcagggc gccctgcgcg aggtcggtgg
     1801 ccaggtggcc gcccgacagc gcggccatgg ccttcccgtc gacgtccctc acgagcgtcc
     1861 gcacgtcacc gcgggccgga ggacgggagc gcgagcgtcg gcgggcacgg ccgcgagggt
     1921 agccgcccgg gacgcccccg gcgcgggcga gggagcggcc tcagcgcacc caggcgccgc
     1981 gccgcaggac gcgctcgacc gtcccgtcct cacgaaggag cacgaggtcg gcgggcgccc
     2041 cgggctcgag gccgcccggg agcccgacga gccgcgcggg ccgctcggag gcggccgcga
     2101 gggcctcggc gagcggcgcg cccgtgaacg cgctccagcg gcgcacggcc tcgtcgagcg
     2161 tcagcgcgct cccggcgagg acgccccgct cgtccgtgac gcggccgccc tgcgcgcgga
     2221 tctcgacgcc ggccatccgg tagctgccgt ccgccgcgcc ggccgcgggg ctcgcgtccg
     2281 tgatcagcac gacgcggcga cgcgcgagcc ggtcgacgag ccgcagcgcg accggatcga
     2341 cgtggaggcc gtccgcgatc accgagacgc gcatcctgct cgcggcgagc gcccaccccg
     2401 gcacgttggg gctccggtgg tggaagtcct tcatcccgtt gaagaggtgg gtcacgagcg
     2461 acgcgcccgc cgcggcggcc cgcgcggcgt cctccgccga gccgccggtg tggccgatcg
     2521 cgacggcgat cccgcgccgc cgcagggcgg cgacgaggtc gagcgcgccg ggcagctccg
     2581 gcgcgagcgt gacgagccgg atcgcgggcg aggcgtagta cggcggctcg ccgtcggcgg
     2641 gagcggcgag acgctcggcc ggatggacgc cgcggtgcga cgggttgagg aacggcccct
     2701 cgaggtggat cccggcgacg ggagagcgcg ggtcgtcggc gcgctcggcc agctcgcgca
     2761 cggcctcctc ggccacttcg ggcgccgtcg agatgaccgt cggcaggtag ctcgtcaccc
     2821 cgtggtcgag ctggagcgcg tcgatcgcgt cgagggcggc agcgccgtcc gtgacgttcg
     2881 cgccggcgcc gccgttcacc tggaggtcgc agaggcccg
//
LOCUS       DASTFC010000158        16416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1535649,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000158 DASTFC010000000
VERSION     DASTFC010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535649"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFO88_04710"
     CDS             <1..286
                     /locus_tag="VFO88_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544855.1"
                     /translation="VRVGARRRSDAVELRVTDEGEGFAPVDRQRIFTKFFRAHGAEPG
                     DEHGTGLGLFLSRGLVAAMGGRMWVESDQGAGASFVFELPVSERAGAGRE"
     gene            289..735
                     /locus_tag="VFO88_04715"
     CDS             289..735
                     /locus_tag="VFO88_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9544856.1"
                     /translation="MPERTTVLVIDDEAPIRLLCRVNLEAEGMAVLEAPDGTKGVELA
                     RAETPDAILLDVMMPGLDGWAVAEELLGDERTSDIPIVFLTARADLRDRARGLDVGGL
                     DYITKPFNPIELASLVRSVVSAVESGDRETLRAERIAELRSLYESA"
     gene            complement(686..1690)
                     /locus_tag="VFO88_04720"
     CDS             complement(686..1690)
                     /locus_tag="VFO88_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9544857.1"
                     /translation="MVDSPVQPAPPEEPRAERPLVGYVLVWSAVALWSLNATVSKVIL
                     ESAGLSALRLSQVRATGAALLLLAAVALWRPASLRIRSWRELAFLAVFGIAGLAFVQL
                     FYFVAIQRLEIGIALVIQYLTPVFVALWARFVEREPVRKRLWGAIALALAGLTLVVDL
                     WGGMTLDGLGVAACLAAAVAYAGYVLMAERALAAGRDQYSLLAWGFAFAALFWAVVQP
                     WWTFPVGSVDGSAPLLGRLDDLEAPVALLIGYVVVLGTVVPFVLLVTALHSLRATRVT
                     VLAMLEPVLAAVVAFAWLGEELTTLQAFGCLLVLAGVGIAQTARPTRTGSEAPRSARP
                     "
     gene            1837..2952
                     /locus_tag="VFO88_04725"
     CDS             1837..2952
                     /locus_tag="VFO88_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET9544858.1"
                     /translation="MLVVLGLTFFALAALMWPSQASAQIACEPPGCRPLCESGFDADS
                     NQTVPLYTLTAGRTGAGDGTFAVTALNPYAGATYNSPANKDRECAKDEDMNEIWDVEH
                     WCTFHCEHRHYFVQPGGLPQPATAWVTATADGSSHFLGWTVLDEACRPPAALSRSKLT
                     CVQTMDEHKTVTANFGTSPDATPPSAPTIARTGTNRNSVTFAITTASTDETWLGGYEI
                     FVAPQTDPRVLRTRVGPSATTFTVANLLCSTDYTFQVEAYDSASSTPSNEVTAKTNSC
                     PKVAPNAVFHLKPPRRTRQKSAFFHWGARRGGVDLPQHAFKSRCKVDKRRWTKCSAVS
                     GKTVRRLKPGWHTFRVKVGDAQGWDRTPAIWRWQVRR"
     gene            complement(2975..3376)
                     /locus_tag="VFO88_04730"
     CDS             complement(2975..3376)
                     /locus_tag="VFO88_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9544859.1"
                     /translation="MTTPQSIRPLVLVADDDEDILALVTFSLRRDGYEVVQARDGAEA
                     LELARERRPAALVLDVMMPKASGFDVTRALRAEGNDVPILLLTASVQERHRARGLAAG
                     ASDFVRKPFSPRELLERVARLVGAREEATFA"
     gene            complement(3373..6048)
                     /locus_tag="VFO88_04735"
     CDS             complement(3373..6048)
                     /locus_tag="VFO88_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544860.1"
                     /translation="MTWSKRPTRLAPPRPGRRRLVTAFWLAAAATLALQGLAYAAPSA
                     ARDELFAVTWIAAAGVAVATALVCVRMTVAPLRRSWLIWAGAVGTWLTGAALQLAAAL
                     TDAHEFEIAADLAWLVLPVVAGLSIAFDAPRGSLSFGLFLLDAIPIVLLVTVLVRFAT
                     PHPLEQMHAHELLHLGFPLLYVLVALVGIQLLMLERDRLRSPNLWGFGIAQPLLAVGA
                     IGWPLVSQGAAAHWFPWEAFWTLGMLTAGGAAVHRASKPFDVPELRPLDENGLRAIPT
                     ALGVLGLLVVTAVAPADYREVPAALAVLSLVCLTARAIVVQREHRLAHATLEENAEKL
                     RTLVSNIPGVVYRCRLDAAWTIEFMSDEIESLTGYPADHFVHNRLQSFGSIEHAEDTE
                     ALNRHIRGRVEAGRPYAYEYRIVRADGEIRWVLDRGQAAEGRDGPLWVDGVLLDVSER
                     KAAEERLERQTELLWLLQRVAVAANEAREPEEALQVAADEVCASTGWPLGHVLTRPEG
                     TDELVSTGIWHADDPDATAAFREVTEALVFTPGVGLPGRVLASRHAEWVEDFGVETHS
                     PRHPHAQALDYRAAFAFPVLVGTEVVGVLEFFAPDSAGRDENLLRIMAHIGTVLGRVV
                     ERTRAEHALAEQNEALRQLDGLKDEFVSLVSHELRTPLTSIRGYLELVLDDAETPLEA
                     QHRAFLDVVQRNADRLLRLVGDLLFVAQVDAGRLIVERAEVDLAAVAAECVKAAEPIA
                     ARREVRVAVDARPTVLVGDRARLGQLLDNLVSNAIKFSPEGGEVSVRVAAAGERAELE
                     VSDGGIGIPADEQEQLFERFFRSSNARRAAIQGTGLGLVIVRAITEAHGGTVAVTSRE
                     GEGTTFRVELPLAAPPPDISADESAPAERALQEAV"
     gene            complement(6128..6745)
                     /locus_tag="VFO88_04740"
     CDS             complement(6128..6745)
                     /locus_tag="VFO88_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544861.1"
                     /translation="MSTRCLLADDHPALVVALSDFLERSGIDVVAIARDGAEAVELAQ
                     AESPDVALVDYRMPRLAGTELIEGIRSAAPGTKVVIYTAEMGDSLARDALGAGASGVV
                     LKEAPLVDILRAVDTALQGRTYVDPALAGQALLNPRAGGTLLTPRERQVLELLAEGLS
                     HDEIGKRLGISGETVRTHLQKASERLGAGTRTQAVATALRLGLIS"
     gene            complement(6742..8124)
                     /locus_tag="VFO88_04745"
     CDS             complement(6742..8124)
                     /locus_tag="VFO88_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9544862.1"
                     /translation="MSDRILVIEDEEIVRGLIVEVLEDAGFEVVAAERAEAGLEVLSR
                     SPISLVVSDIVMPGLTGLDLLARVRATHPSLPVVLVTGAGTYGNLTQALANGASGIVV
                     KPFSHADLVAAVHSALGRARAAENDLRDRIVTPTVAAALANAIEARDVATHGHCERLA
                     ELALRIAEELELSPGDCETVRLGAVLHDVGKIAIPDRILLKPGPLAPEERALIQTHPV
                     VGDRLLEPLAPLEEVRAAVRHHHERWDGTGYPDRLEGEAIPRTARIISVADAIEAMSA
                     TRPYRGPLDPERIVAELEAGRGTQWDPVPVDVALGLLGSGALTFPEGGLTLAAPPAAE
                     SNGAYSVLLVEDDADHAHLVAETIRRALDGAHVVHASHVAQAERLLADCSWSLAVLDH
                     RLPDGNGLDLLRTVKSADPDVPVLVMTGEGSERLAVDAFRLGASDYVVKGENSMTELQ
                     ARVRALLEAA"
     gene            complement(8121..9992)
                     /locus_tag="VFO88_04750"
     CDS             complement(8121..9992)
                     /locus_tag="VFO88_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544863.1"
                     /translation="MPGRIAPFSPAESEPSTEEGRRPGAAEERPLDKALQALTDAALV
                     ALDADSSAAVVWDDRRDFGVIRAASGIAAPFTGTEVGPDAGPHHPREVATVVEAPIEG
                     FLPTRVVVTAGWREPLAPEAERRAAETLRALARVAHRSAEAELEAARLRERARLDTVL
                     GCVGDGVWLESDEGPIVNQAARELLKLGPEDSVRDLDPTLRRLDGTPIPEEERPRALA
                     VATQAFVPFVYQRARKDGVLRVFQGTAAPFYGSSHGDPIGTAVTFHDVTEEYNRNLLT
                     ERFLEQLFEALPIAVGVCDPATGEVLSANQAFGTLVGWEPVEMLGALPPHPWWAEPPD
                     LAAEEPPRAREALFRRRDGRLVPVEIMALLVSEASGRPAAAVTLITDLSERRRFEQQM
                     VQSGRLAAIGELAAGVAHEINNPLFAILGLVEFLLKEVEEGTKPHDRLKLIQQTGLEI
                     KDVVRALLDFARERADELELVELADVLGQTVDLVRRTTLRKEVEIVERYGDEPVVAYA
                     SAGQVKQVVLNLITNAQQAMGLSGTIAIELTRERDHAVVRVTDTGPGIAPDIIDRIFD
                     PFFTTKRELGGTGLGLALSQSIADAHGGTLSAASPAGGGAEFTFTLPLGEPHAEEAA"
     gene            10077..10724
                     /locus_tag="VFO88_04755"
     CDS             10077..10724
                     /locus_tag="VFO88_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544864.1"
                     /translation="MSDSTPGSAKPVTVIVADDHPAMIEAICDTLAEEGLEIVGRAVD
                     GEEALAKIEKRGPTVAVIDLRMPRISGIEATRRLARTAPDTAVVLYTAYGDRALLTEA
                     IDAGARGFVLKEAPLADLVRAVQLVADGGTYVDPVLAGALATAETTATLPALTQRERD
                     VLRLLADGLTNEEIGATLFISPETVRTHIRKAMTKLDAETRTQAVAIALRQSLIA"
     gene            10792..11325
                     /locus_tag="VFO88_04760"
     CDS             10792..11325
                     /locus_tag="VFO88_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9544865.1"
                     /translation="MPVPDHLHDKRRVYEAIRSEDVDGLIEALTDPVNRPWAARHLGK
                     LGDPRAVRPLIRLLGVKDFQARAAAAQALGRLRALEAVEPLLACVEEAPEAVMRAWAI
                     DALGQIGSEAAVPKLLQLLADEHEGLRRTAALALAKIGDPRAVDALEETARRESWLAG
                     RHYRRMARRARKAAAAA"
     gene            complement(11346..11420)
                     /locus_tag="VFO88_04765"
     tRNA            complement(11346..11420)
                     /locus_tag="VFO88_04765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11383..11385),aa:Pro,seq:tgg)
     gene            complement(11795..13321)
                     /locus_tag="VFO88_04770"
     CDS             complement(11795..13321)
                     /locus_tag="VFO88_04770"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9544866.1"
                     /translation="MTSSLPSTPEETAQPEPGKPTVVLGGGPAGLTAGYLLAKRDLPV
                     IVLEAEDQVGGLAKTVVRDGYRFDLGGHRFFTKAKEVERLWHEVMGDEFLLRPRMSRI
                     YWNKKFLDYPLNGRDVVKKLGPVELTRSVGSYLWAAVKPKGKEETFEDWVSNRFGRRL
                     FELFFRSYTEKVWGVPTSELRAEWAAQRIKGLSFFSAAKAAFFGNEGNKVKSLIQEFH
                     YPRYGPGQMWETMTDEIRALGGDVRLESPVERIVVEDGVVREIVVAGGERIEPGHVIS
                     SLPLRTTVAITEPAAPGEVAEAARGLRYRDFLTVALVLDGEDLFPDNWIYIHDPSVRV
                     GRIQNYRSWSPWMVPDESKACVGLEYFCFAGDDLWTMDDDALVELATRELEALGLASR
                     DKVERGYAVRVPKAYPMYDADYAERVDAIRAWLEGIENLQQVGRNGLHRYNNSDHSML
                     TAIRAVENAVLGTNHDIWSVNAESVYHEEDVAEEHPYRDAPRTPELASDEPAPATRVP
                     "
     gene            complement(13342..14901)
                     /locus_tag="VFO88_04775"
     CDS             complement(13342..14901)
                     /locus_tag="VFO88_04775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9544867.1"
                     /translation="MTEVLDRAAWRARAGEQTVIVAKTAVLLVALLAVSLFLRTRALD
                     AGYWIDEGLSVGIAQFPLSEIPGALRQDGSPPLYYMLLHLWIDWFGAGEQATHAFSLL
                     FALATIPVALWAGWSLFGRRAGWVAATLAALNPFLTVYAQETRMYSLVILLSLVLTAA
                     FLHAFAFRRRAYLPVFAASSALLLYTHNWAIFLLVGSLAGLVAIERLVGNRRALARDA
                     ALAYGAAAIAYLPWLPTLVYQAFNTGAPWSKAPSPVELVGGFGIVLAGQGALVAILLA
                     GGIGLRELLIGDRSPARTAVVAALVLALATIATGWVFSQFTPAWANRYLGVLIGPTIL
                     VAAACLPRAGRLGAVALGLVVIFWLPFTASSNKSNPDRLAELFAATIEPGDVILSTHP
                     EQVPVLAHYFGREHAYATPLGPVPDTRVMDWRNALERLEAASPETTLDPLVAGLEPGA
                     RIVLVRPVFENDAQWTAEWTKLVRTRSEQWTTALAADHRLTRTREYVPPYTDASRQTL
                     AMEFFEKAPTG"
     gene            complement(14898..16334)
                     /locus_tag="VFO88_04780"
     CDS             complement(14898..16334)
                     /locus_tag="VFO88_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544868.1"
                     /translation="MAATNVLERTRDAPAGRERARPRPLDLPAWALAVLSVIVAGLSL
                     LLPSAPTYDPWAWIIWGREITELDLDTVYGPSWKPLPVLFTTLFAPFGDAAPLLWVVV
                     ARAGALLGLLLAFRVAYRLAGGGLPGAAGGLAGLLALALSGGWVRNGLMANSEGLLVA
                     VVLWAVDRHLDGRYRQALVLGFAGALLRPEIWPFLGAYGVWLWLRDRPARPLVAGLAL
                     LVPALWFLPELWGSGDPLRASSRARTPDLGTVAFADRPALEVTEGALGLLPLAVLAGA
                     GIALAIVGFRRGRENDVLLALGLGAIGWIAIIAVMTEAGYAGNPRYAVLAAAVFCILA
                     GVAVAWAVRFVAARAGPLAGGAAALVLLGGSLALAGDELRAVDRALEENRSQAALADT
                     LPGAIAAAGGPEELLACGKPYTGKYRVPLLAWHLGLHIREVTVIPELPAVLYYAHGSP
                     PLGTGRLPFTTLAQEGPWTIMAACEAPS"
BASE COUNT         2387 a         5792 c         6188 g         2049 t
ORIGIN      
        1 cgtgcgtgtc ggcgcgcgcc gccggtccga cgcggtcgag ctccgcgtga cggacgaggg
       61 cgaggggttc gcaccggtcg accgtcagcg gatcttcacg aagttcttcc gcgcgcacgg
      121 cgccgagccg ggcgacgagc acggcaccgg gctcggcctc ttcctctcgc gcgggctcgt
      181 cgccgcgatg ggcgggcgca tgtgggtcga gtccgaccag ggcgcgggcg cgagcttcgt
      241 gttcgagctg cccgtctcgg agagggccgg agcgggtagg gagtgacggt gcccgagcgg
      301 acgaccgtgc tcgtgatcga cgacgaggcg ccgattcggc ttctctgccg cgtcaacctc
      361 gaagccgagg gcatggccgt gctcgaggcc ccggacggga cgaagggggt cgagctcgcg
      421 cgcgccgaga cgcccgacgc gatcctcctc gacgtgatga tgccgggact cgacggctgg
      481 gcggtcgccg aggagctgct cggcgacgag cggacgagcg acatcccgat cgtgttcctc
      541 accgcgcgcg ccgatctccg cgaccgcgcg cgcggcctcg acgtcggcgg gctcgactac
      601 atcacgaagc cgttcaaccc gatcgagctc gcgtcgctcg tccgcagcgt cgtctccgcg
      661 gtcgagtcgg gcgaccgcga gaccctcagg gccgagcgga tcgcggagct tcgctccctg
      721 tacgagtcgg cctagccgtc tgggcgatcc cgacgccggc gaggacgagg aggcagccga
      781 acgcctggag ggtcgtcagc tcctcgccga gccacgcgaa cgcgacgacc gcggcgagca
      841 cgggctcgag catcgcgagg acggtcacgc gcgtcgcgcg gagcgagtgg agcgcggtca
      901 cgaggaggac gaacgggacg accgtcccga gcacgacgac gtaaccgatg agcagcgcga
      961 ccggcgcctc caggtcgtcg agccggccga gcaggggcgc cgagccgtcc acggatccga
     1021 ccgggaacgt ccaccacggc tggacgaccg cccagaagag cgcggcgaac gcgaagcccc
     1081 aggcgaggag cgagtactgg tcgcgcccgg cggcgagcgc ccgctccgcc atgaggacgt
     1141 acccggcgta cgcgaccgcg gccgcgaggc aggccgcgac gccgaggccg tcgagcgtca
     1201 tcccgcccca gagatcgacg acgagcgtga gcccggcgag cgcgagcgcg atcgcgcccc
     1261 agagccgctt gcgcacgggc tcgcgctcga cgaaccgagc ccacagcgcg acgaagacag
     1321 gcgtcaggta ctggataacg agcgcgatcc cgatctcgag ccgctggatc gcgacgaagt
     1381 agaagagctg cacgaacgcg agcccggcga tcccgaagac ggcgaggaac gcgagctcgc
     1441 gccacgatcg gatccgtagc gacgccggcc gccagagggc gaccgccgcg aggaggagga
     1501 gcgccgcgcc ggtcgcgcgc acctgcgaga gccggagcgc cgagaggccc gccgactcga
     1561 ggatcacctt cgagacggtc gcgttcagcg accagagcgc gacggcggac cagacgagga
     1621 cgtagccgac gagcgggcgc tcggcgcgcg gctcctcggg cggggcgggc tggacggggc
     1681 tgtcgaccac ggccgcgcag cgtactgctc ggaccgcgcg gtaacgaaat cgcaagcggg
     1741 agcggtccgg agacctgcac ggtggtactg tctgccgggg aacgcaggcg tttgacggga
     1801 cgggagacaa cggcgaacga cctggccagg cgtgtcgtgc tggtcgtcct cggcctgacg
     1861 ttcttcgcgc tcgcggcgct catgtggccg tcgcaggcga gcgcccagat cgcgtgcgag
     1921 ccgccgggct gccggccgct ctgcgagtcc ggcttcgacg ctgactccaa tcagaccgtc
     1981 ccgctgtaca cgctgaccgc cggccggacc ggcgccgggg acggcacctt cgcggtgacg
     2041 gccctcaacc cctacgcagg cgcgacctac aactccccgg cgaacaagga cagggagtgc
     2101 gcaaaggacg aggacatgaa cgagatctgg gacgtggagc actggtgcac gttccactgc
     2161 gagcaccgcc actacttcgt ccagcccggt gggcttcccc agcccgcgac ggcgtgggtc
     2221 acggcgaccg cggacggctc gtcccacttc ctcggctgga cggtgttgga cgaggcctgc
     2281 cgcccgcccg cggcgctctc gcgctcgaag ctcacgtgcg tgcagacgat ggacgagcac
     2341 aagaccgtga cggccaactt cgggacgagc ccggacgcga caccgccgag cgcgccgacg
     2401 atcgcgcgca cggggacgaa caggaactcg gtcacgttcg cgatcacgac cgcctcgacg
     2461 gacgagacct ggctcggcgg ctacgagatc ttcgtcgccc cccagaccga tccgcgagtc
     2521 ctccgcacgc gggtcggccc cagcgcgacg acgttcaccg tggcgaacct tctctgcagc
     2581 acggactaca cgttccaggt cgaggcctac gactcggcga gctcgacacc gagcaacgag
     2641 gtcaccgcga agacgaactc gtgcccgaag gtggcgccga acgcggtctt ccacctgaag
     2701 ccgccgcgga ggacgaggca gaagtccgcg ttcttccact ggggcgcgcg ccgcggcggg
     2761 gtcgacctcc cgcagcacgc cttcaagtcg cgctgcaagg tggacaagcg ccggtggacg
     2821 aagtgcagcg ccgtgagcgg caagaccgtc cgcaggctca agccgggctg gcacaccttc
     2881 cgcgtgaagg tcggcgacgc gcagggctgg gaccgcacgc cggcgatctg gcgctggcag
     2941 gtccgccgct agcgggccgc ggacggcgga gggctcacgc gaaggtcgcc tcctcgcggg
     3001 cgccgacgag ccgggcgacg cgctcgagga gctcgcgcgg gctgaacggc ttgcgcacga
     3061 agtcgctcgc gccggcggcg agcccgcgcg cgcgatgtcg ctcctgcacg ctcgcggtga
     3121 ggagcaggat cggcacgtcg ttcccctcgg cgcgcagcgc ccgggtgacg tcgaagccgc
     3181 tcgccttcgg catcatcacg tcgaggacga gcgcggccgg ccgccgctcg cgggcgagct
     3241 cgagcgcctc ggcgccgtcg cgcgcctgga cgacctcgta gccgtcgcgg cggagcgaga
     3301 aggtgacgag ggcgaggatg tcctcgtcgt cgtcggcgac gaggacgagg ggcctgatcg
     3361 actgcggcgt ggtcatacgg cctcctgaag agctcgttcc gccggcgcgg attcgtcggc
     3421 ggagatgtcg ggcgggggcg ccgcgagcgg gagctcgacg cggaacgtcg tgccctcccc
     3481 ctcgcggctc gtgacggcga cggttccccc gtgggcctcg gtgatcgccc ggacgatcac
     3541 gagcccgagg cccgtcccct ggatcgccgc gcgccgcgcg ttcgaggagc ggaagaaccg
     3601 ctcgaagagc tgttcctgct cgtccgccgg gatcccgatc ccgccgtccg agacctcgag
     3661 ctcggcgcgc tcgccggcgg cggccacgcg cacggacacc tcgccgccct ccgggctgaa
     3721 cttgatcgcg ttcgagacga ggttgtcgag gagctgcccg aggcgggccc ggtcgccgac
     3781 gaggacggtc ggccgggcgt ccacggcgac gcggacctcg cgccgggcgg cgatcggctc
     3841 cgccgccttc acgcactcgg cggcgacggc ggcgaggtcg acctcggcgc gctcgacgat
     3901 gagccgcccg gcgtcgacct gcgcgacgaa gagcaggtcg ccgacgaggc ggagcagccg
     3961 gtcggcgttg cgctgcacga cgtcgaggaa ggcgcggtgc tgcgcctcga gcggcgtctc
     4021 ggcgtcgtcg agcacgagct cgaggtagcc gcggatcgag gtcagcgggg tgcggagctc
     4081 gtgcgagacg agcgagacga actcgtcctt caggccgtcg agctggcgca gcgcctcgtt
     4141 ctgctccgcg agggcgtgct ccgcgcgggt gcgctcgacg acgcggccga ggacggtgcc
     4201 gatgtgggcc atgatcctga ggaggttctc gtcgcgcccg gccgagtccg gcgcgaagaa
     4261 ctcgagcacg ccgacgacct ccgtcccgac gaggaccggg aaggcgaacg cggcgcggta
     4321 gtcgagcgcc tgcgcgtgcg ggtggcgcgg cgagtgcgtc tcgacgccga agtcctccac
     4381 ccactcggcg tggcgcgagg cgaggacacg acccggcagg ccgacgccgg gcgtgaagac
     4441 gagcgcctcc gtgacctccc ggaacgcggc cgtggcgtcg gggtcgtccg cgtgccagat
     4501 cccggtcgag acgagctcgt ccgtgccctc cggccgcgtg agcacgtggc cgagcggcca
     4561 gccggtcgac gcgcagacct cgtccgccgc gacctggagt gcctcctcgg gctcgcgcgc
     4621 ctcgttcgcc gcgacggcca cgcgctggag gagccagagg agctccgtct ggcgctccag
     4681 ccgctcctcg gcggccttcc gctcgctgac gtcgaggagg acgccgtcga cccagagcgg
     4741 cccgtcgcga ccctcggcgg cctgcccgcg gtcgagcacc cagcggatct ccccgtcggc
     4801 gcggacgatg cggtactcgt acgcgtacgg tctccccgcc tcgacgcggc cgcggatgtg
     4861 gcggttgagg gcctccgtgt cctcggcgtg ctcgatcgac ccgaacgact gcaggcggtt
     4921 gtggacgaag tggtcggcgg ggtagccggt gagcgactcg atctcgtcgc tcatgaactc
     4981 gatcgtccac gcggcgtcga ggcggcaccg gtagacgacg ccggggatgt tcgagacgag
     5041 cgtccggagc ttctcggcgt tctcctcgag ggtcgcgtgc gcgaggcggt gctcccgctg
     5101 gacgacgatc gcgcgggcgg tgagacagac gagcgagaga acggcgagcg ccgccggaac
     5161 ctcgcggtag tcggcgggcg cgacggcggt gacaacgagc agccccagca cgccgagcgc
     5221 ggtcgggatc gcgcggaggc cgttctcgtc gagcgggcgg agctccggga cgtcgaacgg
     5281 cttggaggcc cggtgcacgg cggcgccgcc ggcggtgagc atgccgagcg tccagaacgc
     5341 ctcccacggg aaccagtgcg ccgccgctcc ctggctcacg agcggccagc cgatcgcgcc
     5401 gacggcgagg agcggctggg cgatgccgaa gccccagagg ttcggcgagc gaagccggtc
     5461 tcgctcgagc atgagcagct ggatcccgac gagcgcgacg agcacgtaga gcagcgggaa
     5521 gccgaggtgg agcagctcgt gcgcgtgcat ctgctccagc gggtgcggcg tcgcgaagcg
     5581 gacgaggacg gtgacgagca ggacgatcgg gatcgcgtcc agcaggaaga ggccgaagga
     5641 gagcgacccg cgcggcgcgt cgaaggcgat cgagaggccg gcgacgacgg ggaggacgag
     5701 ccacgcgagg tccgcggcga tctcgaattc gtgggcgtcg gtgagcgcgg ccgcgagctg
     5761 gagcgcggcg cccgtgagcc aggtgccgac cgcgcccgcc cagatgagcc acgagcggcg
     5821 gagcggcgcg accgtcatcc gcacgcagac gagcgccgtc gcgacggcga cccccgcggc
     5881 ggcgatccac gtgacggcga agagctcatc ccgagcggcc gacggcgccg cgtacgcgag
     5941 cccctggagc gcgagcgtgg cggccgctgc gagccagaag gccgtgacga ggcgccggcg
     6001 gccggggcgc ggcggcgcaa ggcgcgtcgg gcgcttcgac cacgtcaccg tcgcactgtc
     6061 gggccggcgg gcccgtccgt catcctccgc gcgtcgcatt cgcagtcccc gtttcgcggc
     6121 gcgcgcccta cgagatcagc ccgaggcgca gggcggtcgc gaccgcctgg gtgcgcgtgc
     6181 cggcgccgag ccgctcggag gccttctgga ggtgggtccg caccgtctcg ccgctgatcc
     6241 cgagccgctt gccgatctcg tcgtggctca ggccctcggc gaggagctcg agcacctggc
     6301 gctcccgcgg agtcaggagc gtcccgcccg cgcgcgggtt gaggagcgcc tgcccggcga
     6361 gtgcgggatc gacgtacgtg cgcccctgga gcgccgtgtc gacggcgcgc aggatgtcca
     6421 cgagcggcgc ctccttcagg acgacgcccg acgcgccggc gccgagggcg tcccgggcga
     6481 ggctgtcgcc catctcggcc gtgtagatca cgaccttcgt cccgggcgcg gccgaacgga
     6541 tcccctcgat gagctccgtg ccggcgagcc tcggcatccg gtagtcgacg agcgcgacgt
     6601 cggggctctc ggcctgggcc agctcgaccg cctcggcgcc gtcgcgcgcg atcgcgacga
     6661 cgtcgatgcc gctcctctcg aggaagtccg agagcgccac gacgagcgcg ggatggtcgt
     6721 cggcgaggag gcagcgcgtg ctcacgccgc ctccaggagc gcccggacac gggcctggag
     6781 ctcggtcatc gagttctctc ccttcacgac gtagtcggag gcgccgaggc ggaacgcgtc
     6841 caccgcgagg cgctcggacc cttcgccggt catgacgagg acggggacgt cgggatccgc
     6901 gctcttcacc gtccggagga ggtcgagccc gttcccgtcc ggaagccggt ggtcgaggac
     6961 ggcgagcgac cacgagcagt cggccaggag gcgctcggcc tgcgcgacgt ggctcgcgtg
     7021 gacgacgtgc gcgccgtcga gcgcgcgccg gatggtctcc gcgacgaggt gcgcgtggtc
     7081 ggcgtcgtcc tccacgagga ggacggagta cgcgccgttc gactcggcgg ccggcggcgc
     7141 cgcgagcgtg aggccgccct ccgggaacgt gagcgcgccg gagccgagga gcccgagcgc
     7201 gacgtccacg ggaaccgggt cccactgcgt gccgcggccg gcctcgagct cggccacgat
     7261 ccgctcgggg tcgagcggcc cgcggtacgg ccgcgtcgcc gacatcgcct cgatcgcgtc
     7321 ggcgacggag atgatccgcg cggtgcgggg aatggcctcg ccctcgagcc ggtcggggta
     7381 gccggtgccg tcccagcgct cgtggtggtg gcgcacggcg gcgcgcacct cctcgagcgg
     7441 cgcgagaggc tcgagcagcc ggtcgccgac gacggggtgc gtctggatga gcgcgcgctc
     7501 ctcgggcgcg agcgggcccg gcttcaggag aatgcggtcg gggatcgcga tcttgccgac
     7561 gtcgtggagc acggcgccca ggcgcacggt ctcgcagtcg ccgggggaga gctcgagctc
     7621 ctccgcgatt cgcagggcga gctcggcgag ccgttcgcag tggccgtggg tcgcgacgtc
     7681 gcgggcctcg atcgcgttcg cgagcgcggc ggcgaccgtc ggggtgacga tccggtcgcg
     7741 gaggtcgttc tccgcggcgc gggcgcgtcc gagcgcggag tgcacggcgg cgacgaggtc
     7801 ggcgtgcgag aacggcttga cgacgatccc gctcgccccg ttcgcgagcg cctgcgtgag
     7861 gttcccgtac gtgcccgcgc cggtcacgag gacgacgggg aggctcgggt gcgtcgcccg
     7921 gacgcgggcg agcaggtcga gcccggtcag gccgggcatg acgatgtcgc tgacgacgag
     7981 cgagatcggg ctccgcgaga gcacctcgag gcccgcctcg gcgcgctccg ccgcgacgac
     8041 ctcgaacccg gcgtcctcga gcacctcgac gatgagcccg cggacgatct cctcgtcctc
     8101 gatcacgagg atccggtcgc tcacgccgcc tcctccgcgt gcggctcgcc gagggggagc
     8161 gtgaacgtga actcggcgcc gccgccggcg ggcgaggcgg ccgagagcgt cccgccgtgg
     8221 gcgtccgcga tcgactggct gagcgcgagc ccgagcccgg tgccgccgag ctcgcgcttc
     8281 gtcgtgaaga acgggtcgaa gatccggtcg atgatgtccg gggcgatccc gggccccgtg
     8341 tccgtgacgc ggacgaccgc gtgatcccgc tcgcgcgtga gctcgatcgc gatcgtcccg
     8401 ctcaggccca tcgcctgctg ggcgttcgtg atcaggttga ggacgacctg tttcacctgg
     8461 ccggcgctcg cgtacgcgac gaccggctcg tcgccgtagc gctcgacgat ctcgacctcc
     8521 ttgcggagcg tcgtccggcg cacgaggtca accgtctggc cgagcacgtc cgcgagctcg
     8581 accagctcga gctcgtccgc gcgctcgcgc gcaaaatcga ggagcgcccg gacgacgtcc
     8641 ttgatctcga gcccggtctg ctggatgagc ttcagccggt cgtgcggctt cgtgccctcc
     8701 tcgacctcct tgagcaggaa ctcgacgagc ccgaggatcg cgaagagcgg gttgttgatc
     8761 tcgtgcgcga cgccggccgc gagctcgccg atcgccgcga ggcgcccgct ctgcaccatc
     8821 tgctgctcga agcgccgccg ctcggagagg tcggtgatga gcgtgacggc cgcggcgggg
     8881 cggccgctcg cctcgctcac gaggagcgcc atgatctcga cggggacgag ccggccgtcg
     8941 cggcggcgga agagcgcctc gcgggcgcgc ggcggctcct cggcagcgag gtccggcggc
     9001 tcggcccacc acgggtgcgg gggaagcgcg ccgagcatct cgaccggctc ccagccgacg
     9061 agcgtcccga aggcctggtt cgcggagagc acctcgccgg tggccgggtc gcagacgccg
     9121 acggcgatcg gcagcgcctc gaagagctgc tcgaggaagc gctcggtcag gaggttgcgg
     9181 ttgtactcct ccgtcacgtc gtggaaggtc acggcggtgc cgatcgggtc gccgtgcgac
     9241 gagccgtaga acggcgcggc ggtgccctgg aagacgcgca ggacgccgtc cttgcgtgct
     9301 cgctggtaca cgaacgggac gaacgcctgc gtcgcgaccg cgagcgcgcg cggcctctcc
     9361 tcctcgggga tcggcgtccc gtcgaggcgg cggagggtcg ggtcgaggtc gcgtacggag
     9421 tcctccgggc cgagcttcag gagctcgcgg gcggcctggt tcacgatcgg gccctcgtcg
     9481 gactcgagcc agacgccgtc gcccacgcac ccgagcaccg tgtcgagccg tgccctctcg
     9541 cgcaggcgcg ccgcctcgag ctcggcctcc gcggagcggt gggcgacgcg ggcgagcgcg
     9601 cggagcgtct ccgcggcgcg gcgctcggct tcgggcgcga ggggctcgcg ccagcctgcg
     9661 gtcacgacga cccgggtcgg gaggaagccc tcgatcggcg cctcgacgac cgtggcgacc
     9721 tcgcgcgggt ggtgcggccc ggcgtcgggg ccgacctcgg ttccggtgaa cggggcggcg
     9781 attccggagg ccgcgcggat gacgccgaag tcgcgccggt cgtcccacac gaccgcggcc
     9841 gaggagtcgg cgtcgagcgc gaccagcgcc gcatctgtca gcgcctggag cgccttgtca
     9901 agcggacgct cctccgcggc gccgggccgg cgtccctcct cggtcgaagg ttcgctctcc
     9961 gcaggggaaa atggggcgat ccgtcctggc actctgtaca gttagcggca cggatcgcgc
    10021 aaccgagcat ccccgttgtc gcgtatctct tgtcacccga acgagggacg ccgtacgtgt
    10081 cagactcgac accgggaagc gcgaagcccg tcaccgtgat cgtcgcggac gaccacccgg
    10141 cgatgatcga ggcgatctgc gacacgctcg cggaggaggg gctcgagatc gtcggccgcg
    10201 ccgtcgacgg cgaggaggcg ctcgcgaaga tcgagaagcg cggccccacg gtcgccgtga
    10261 tcgacctccg catgccgcgg atctccggga tcgaggcgac gcgccggctc gcccgcaccg
    10321 cgccggacac ggcagtcgtc ctctacacgg cctacggcga ccgggcgctc ctgacggagg
    10381 cgatcgacgc gggagcgcgc gggttcgtcc tgaaggaggc gccgctcgcc gacctcgtgc
    10441 gcgccgtgca gctcgtcgcc gacgggggca cgtacgtcga tccggtgctc gccggcgcgc
    10501 tcgcgaccgc cgagacgacg gcgaccctcc cggcgctcac ccagcgcgag cgcgacgttc
    10561 tccgcctcct cgcagacggg ctcacgaacg aggagatcgg cgcgacgctc ttcatctcgc
    10621 ccgagacggt gcgcacccac atccgcaagg cgatgacgaa gctcgatgcg gaaacgagaa
    10681 cgcaggcggt cgcgatcgcc ctccgccagt ccctcatcgc gtagccgttg gtcgggactc
    10741 ccgcggttcc acgccctgca gatatctcat cggcttgccg atgaccacga cgtgcccgtt
    10801 cccgaccacc ttcatgacaa gcgacgcgtc tacgaagcga ttcgatctga ggacgtagac
    10861 gggctcatcg aagcactaac ggaccctgtg aaccgaccgt gggccgcgcg gcatcttggc
    10921 aagctcggcg acccacgtgc cgtccggccc ttgattcggc ttctcggtgt caaggacttt
    10981 caggcgcgcg ccgcggccgc tcaggccctc ggacgcctgc gcgcgctcga ggccgtggag
    11041 cctcttctcg cgtgcgtgga agaggcgcct gaagccgtca tgcgtgcttg ggccattgat
    11101 gcactgggcc agatcgggag cgaggcggcg gtccccaagc tcctgcagct gcttgccgac
    11161 gagcacgagg ggcttcgccg aaccgctgcg ctcgcgctgg ccaagatcgg cgacccgcgt
    11221 gcagttgacg cgctcgagga aacggcgcga cgcgagtcgt ggcttgcggg ccgccactac
    11281 cggcgcatgg cgcggcgggc gaggaaggcc gcggccgctg cctgacacgc gcgacgagta
    11341 cggagtcggg acggcgagat tcgaactcgc ggcctccgcc tcccaaaggc ggcgctctga
    11401 ccaggctgag ctacgtcccg tagccggctc atcgtagacg tccgccgagc cggagcgatt
    11461 tcgtcgccgc gcccgacctg gcgggcgcgg ctcaggagcg gtgaaggaac gccgctccta
    11521 cgcgagtcgt catggcaccc gcgaagcggc tgccggactc gtgtcggctg ctcgcggcct
    11581 ccgcctccca aaggcggcgc tctgaccagg ctgagctacg tcccgtagcc cgctcatcgt
    11641 agtctcgagc ccgaccggcg cgctcgccgc tcccgagcga accccttgac tagtaacact
    11701 gtgttactag cgagggtcgg gggcgtgtga gcccgaccta gtaacactgt gttactaggt
    11761 gagggggctc ccggccggtt gcccgcgccg cgtcctaggg aacgcgcgtc gccggcgccg
    11821 gctcgtccga cgcgagctcg ggcgtccggg gtgcgtcgcg gtacgggtgc tcctccgcga
    11881 cgtcctcctc gtggtagacg ctctccgcgt tcacggacca gatgtcgtgg ttcgtgccga
    11941 ggacggcgtt ctcgaccgcg cggatcgcgg tcagcatcga gtggtcggag ttgttgtagc
    12001 ggtggaggcc gttgcggccg acctgctgga ggttctcgat cccttcgagc caggcacgga
    12061 tcgcgtcaac ccgctcggcg tagtcggcgt cgtacatcgg gtacgccttc gggacgcgta
    12121 ccgcgtagcc gcgctcgacc ttgtcgcggg acgcgagccc gagcgcctcg agctcccgcg
    12181 tggcgagctc gacgagcgcg tcgtcgtcca tcgtccagag gtcgtcgccc gcgaagcaga
    12241 agtactcgag cccgacgcag gccttcgatt cgtcgggaac catccacggg ctccaggagc
    12301 ggtagttctg gatccgtccg acgcgcaccg acgggtcgtg gatgtagatc cagttgtccg
    12361 ggaagaggtc ctcgccgtcg agcacgagcg cgaccgtgag gaagtcccgg tagcgcaggc
    12421 cgcgcgccgc ctcggccacc tcgcccggcg ccgccggctc cgtgatcgcg acggtcgtcc
    12481 gcagcggcag cgacgagatc acgtgccccg gctcgatccg ctcgccgccg gccacgacga
    12541 tctcgcgcac gacgccgtcc tcgacgacga tccgctcgac cggcgactcg agcctgacgt
    12601 cgccgccgag cgcgcggatc tcgtccgtca tcgtctccca catctgcccg ggcccgtagc
    12661 gtgggtagtg gaactcctgg atgaggctct tgaccttgtt cccctcgttg ccgaagaacg
    12721 cggccttcgc tgcggagaag aacgagagcc ccttgatccg ctgcgccgcc cactcggcgc
    12781 gcagctcgct cgtcgggacg ccccagacct tctccgtgta ggagcggaag aagagctcga
    12841 agagccggcg gccgaaacgg ttcgagaccc agtcctcgaa cgtttcctcc ttgcccttcg
    12901 gcttcacggc cgcccagagg tacgacccga ccgagcgcgt gagctcgacc ggcccgagct
    12961 tcttgaccac gtcgcgcccg ttcagcgggt agtcgaggaa cttcttgttc cagtagatcc
    13021 gcgacatgcg ggggcgcagg aggaactcgt cgcccatcac ctcgtgccag aggcgctcga
    13081 cctccttcgc cttcgtgaag aagcggtggc cgcccaggtc gaagcggtac ccgtcgcgca
    13141 cgaccgtctt cgcgagcccg ccgacctgat cctcggcctc gaggacgatc acgggcaggt
    13201 cgcgctttgc gagcaggtag ccggcggtca gcccagcggg gccgccgccg aggaccacgg
    13261 tcggcttccc gggctcgggc tgcgcggtct cttcgggtgt cgaggggaga gaggaggtca
    13321 tgcgggtggg acgacgcctt cctacccggt cggcgctttc tcaaagaatt ccatcgcgag
    13381 cgtctggcgg ctcgcatcag tgtacggagg gacgtactcc cgcgtccggg tgaggcgatg
    13441 gtcggcggcg agcgcggtcg tccactgctc ggagcgggtg cggacgagct tcgtccactc
    13501 ggccgtccac tgggcgtcgt tctcgaagac ggggcgcacg aggacgatcc gcgcgcccgg
    13561 ctcgaggccc gcgacgagcg ggtcgagtgt cgtctccggg ctcgcggcct cgagccgctc
    13621 gagcgcgttg cgccagtcca tcacgcgcgt gtccggcacc ggcccgagcg gcgtcgcgta
    13681 cgcgtgctcc cggccgaagt agtgggcgag caccggcacc tgctcgggat gcgtggagag
    13741 gatcacgtcg cccggctcga tcgtcgcggc gaagagctcc gcgaggcggt cggggttgct
    13801 cttgttcgag ctcgccgtga acggcagcca gaagatcacc acgagaccga gcgcgaccgc
    13861 cccgagccgg ccggcgcgcg ggaggcaggc cgcggcgacg aggatcgtcg ggccgatcag
    13921 gacgccgagg tagcggttcg cccacgcggg cgtgaactgg gagaagaccc agccggtcgc
    13981 gatggtcgcg agcgccagga cgagcgcggc gacgaccgcc gtgcgcgccg gcgagcggtc
    14041 tccgatcagg agctcccgca gcccaatgcc gcccgcgagc aggatcgcga cgagcgcgcc
    14101 ctggcccgcg agcacgatcc cgaagccgcc cacgagctcg accggcgagg gcgccttcga
    14161 ccacggggcg cccgtgttga acgcctggta gacgagcgtc ggcagccacg ggagataggc
    14221 gatcgcggcg gcgccgtacg cgagcgccgc gtcgcgggcg agcgcccgcc ggttcccgac
    14281 gagccgctcg atcgcaacga gcccggcgag cgatccgacg aggaggaaga tcgcccagtt
    14341 gtgcgtgtag aggaggagcg ccgagctcgc cgcgaagacc ggcaggtacg cgcgccggcg
    14401 aaacgcgaac gcgtggagga acgccgccgt caggacgagg gagaggagga tcacgagcga
    14461 gtacatccgc gtctcctgcg cgtagaccgt gaggaacggg ttgagcgcgg cgagcgtcgc
    14521 cgcgacccag ccggcgcggc ggccgaagag gctccacccg gcccagagcg cgaccgggat
    14581 cgtggcgagc gcgaagagga gcgagaacgc gtgcgtcgcc tgctcgcccg ccccgaacca
    14641 gtcgatccag aggtggagga gcatgtagta gagcggcggc gagccgtcct ggcgcagcgc
    14701 gccggggatc tccgagagcg ggaactgcgc gatgccgacc gagaggccct cgtcgatcca
    14761 gtagccggcg tcgagcgccc tcgtgcggag gaagagcgag accgcgagga gcgcgacgag
    14821 gaggacggcc gtcttcgcga cgatcaccgt ctgctcgccg gcgcgggcgc gccaggcggc
    14881 gcgatcgagc acctcggtca cgacggcgcc tcgcaggcgg ccatgatcgt ccacggtccc
    14941 tcctgtgcga gggtcgtgaa cggcaggcgg ccggtaccga gcggcgggga gccgtgggcg
    15001 tagtagagga cggccgggag ctcggggatc acggtcacct cccggatgtg gaggccgagg
    15061 tgccacgcga ggagcggcac acggtacttg cccgtgtacg gcttcccgca ggcgaggagc
    15121 tcctccggcc cgcccgcggc ggcgatcgcg ccggggagcg tgtcggcgag cgccgcctgg
    15181 gagcggttct cctcgagcgc gcggtcgacg gcgcggagct cgtcgccggc gagcgcgagc
    15241 gagccgccga ggaggacgag cgcggcggcg ccgccggcga gcggtccggc ccgcgcggcg
    15301 acgaagcgaa cggcccacgc gaccgcgacc ccggcgagga tgcagaagac ggccgcggcg
    15361 aggactgcgt agcgcgggtt cccggcgtac cccgcctcgg tcatgacggc gatgatcgcg
    15421 atccacccga tcgcgccgag gccgagcgcg agcaggacgt cgttctcccg gccgcggcgg
    15481 aacccgacga tcgcgagcgc gatccccgcg cccgcgagga ccgcgagcgg gaggagcccg
    15541 agcgcgccct ccgtcacctc gagcgcgggc cggtcggcga acgcgaccgt gccgagatcc
    15601 ggggtgcgcg cgcggctgga ggcgcggagc gggtcgccgg agccccagag ctccgggagg
    15661 aaccagagcg ccggcacgag gagcgcgagc ccggcgacga gcggccgcgc cggccggtcc
    15721 cgcagccaga gccacacgcc gtaggcgccg aggaacggcc agatctccgg ccgcaggagc
    15781 gcgcccgcga agccgagcac gagggcctgc cggtagcgcc cgtccaggtg ccggtcgacc
    15841 gcccagagga cgaccgccac gagcaggccc tccgagttcg ccatgagccc gttccgcacc
    15901 cacccgcccg agagcgcgag cgccaggagc cccgcgagcc cgcccgccgc gcccggcagg
    15961 ccgccgcccg cgagccggta ggcgacgcga aacgcgagca ggagcccgag cagcgcgccc
    16021 gcgcgcgcga cgacgaccca gaggagcggc gccgcgtctc cgaagggggc gaagagggtc
    16081 gtgaagagca cgggcagtgg cttccacgac gggccgtaga ccgtgtcgag gtcgagctcg
    16141 gtgatctcgc ggccccagat gatccaggcc caggggtcgt acgtcggcgc ggacgggagg
    16201 aggagcgaga gcccggccac gatgaccgag aggacggcga gcgcccacgc cgggaggtcg
    16261 agcggccgcg gccgggcgcg ctcgcgcccg gccggcgcgt cgcgcgtcct ctcgagcacg
    16321 ttcgtggcgg ccacaggagg tgaacctacc caggcgtttc cagccgcgcc gcccggaagc
    16381 gcgaccaccc gccgtagcgg gtgacgagga aggcga
//
LOCUS       DASTFC010000159         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_464395,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000159 DASTFC010000000
VERSION     DASTFC010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464395"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFO88_04785"
     CDS             complement(<1..344)
                     /locus_tag="VFO88_04785"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET9544869.1"
                     /translation="MDVEIGLGKKARRAYGFDDVAIVPSRRTRDPDDVDIGWKLGDHL
                     LELPLLASAMDGVVSPKTAIEIGKLGGLGVLNLEGIFTRYARAESELERIASFGPHEA
                     TRGMQEIYAKAI"
     gene            complement(469..1428)
                     /locus_tag="VFO88_04790"
     CDS             complement(469..1428)
                     /locus_tag="VFO88_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HET9544870.1"
                     /translation="MIVTVTLNTAMDRTLLVPNFQLGQRHRASLALSSAGGKGVNVAR
                     ALRHLGVPVVATGFLGGRNGAQIADLLTDEGILNDFVRIEGESRTSVALLDPISDQYT
                     EVNEWGPEVSEAELDALREKLAYLTQGAEFVVFAGSLPRGVDPAFYGELVRDLAKQQI
                     LTVLDTEGEPLRLGVGAEPFLVSPNLREAEALVGHEFVDDDDLAHGLDEIAELGARNV
                     LVSLETGCYALVRDDREAHRVRGIAPRLEVVSTIGAGDTLLAGFLAARIAGKDVEESV
                     RAAVAAGAASILEPGPGRFDPRAVSRVSNLVKVERLAPVAQDA"
     gene            complement(1425..>2310)
                     /locus_tag="VFO88_04795"
     CDS             complement(1425..>2310)
                     /locus_tag="VFO88_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544871.1"
                     /translation="STIDLVPGERMSVRDLLAAALIQSANDAAFALAAHVGDGSVPRF
                     VELMNEKARELGLDDTTFVRPDGLDVPGHRSTARDVLVLARAAMEQPLVRRLVRQDSL
                     AIAGGRELFGWNDLLGEYRGLFGVKTGHTDLAGWCQVGVARRDGATVYAVVLGAPTRA
                     RRNDALADLLDWGFDRFARTEVVTTGRAYASADLPYTDERVDLVAEKSAAPVLLLGRP
                     LVETVVAETAVDLPVREGDVLGEVVVRDGERVVARRPLVAASNFEEPGLLERAGWYAG
                     RTLEEAGDMLDAALGAIL"
BASE COUNT          324 a          845 c          833 g          308 t
ORIGIN      
        1 tcgatcgcct tcgcgtagat ctcctgcatc ccgcgcgtcg cctcgtgcgg cccgaacgac
       61 gcgatgcgct cgagctcgct ctccgccctc gcgtagcggg tgaagatccc ctcgaggttg
      121 aggacgccga ggccgccgag cttgccgatc tcgatcgcgg tcttcggcga gacgaccccg
      181 tccatcgccg aggcgaggag cgggagctcg agcagatggt cgccgagctt ccagccgatg
      241 tcgacgtcgt ccgggtcgcg cgtccgccgg ctcgggacga tcgccacgtc gtcgaacccg
      301 tacgcgcggc gcgccttctt cccgagcccg atctcgacgt ccacgctaac gctcctccga
      361 gaacgaaaaa gcccaggcgg accgcgcttg cctgggcacc aggacggagg ttcccgtcgc
      421 cactctcata cggggcgatg ctaacaccgg ctcatgacgt cgccgcgctc acgcgtcctg
      481 ggcgacgggc gcgagccgct cgaccttcac gaggttcgag acgcggctca ccgcgcgggg
      541 gtcgaagcgg cccggaccgg gctcgagaat ggacgccgcg ccggccgcga cggcggcgcg
      601 cacggactcc tccacgtcct tgcccgcgat ccgcgcggcg aggaagccgg cgagcagcgt
      661 gtcgcccgcg ccgatcgtcg agacgacctc gagccgcggc gcgatcccgc gcacgcggtg
      721 ggcctcgcgg tcgtcgcgga cgagcgcgta gcagccggtc tcgagcgaga caaggacgtt
      781 gcgcgcgccg agctcggcga tctcgtcgag cccgtgcgcg aggtcgtcgt cgtccacgaa
      841 ctcgtgcccg acgagcgcct cggcctcgcg gaggttcggc gagacgagga acggctcggc
      901 gccgacgccg agccggagcg gctctccctc ggtgtcgagc acggtgagga tctgctgctt
      961 cgcgaggtcg cggacgagct cgccgtagaa cgcggggtcg accccgcgcg ggagcgagcc
     1021 ggcgaagacg acgaactccg cgccctgggt gaggtaggcg agcttctcgc ggagggcatc
     1081 gagctccgcc tcgctcacct cggggcccca ctcgttgacc tccgtgtact ggtcggagat
     1141 cgggtcgagg agcgcgaccg acgtccgcga ctcgccctcg atccgcacga agtcgttcag
     1201 gatcccctcg tcggtcagga ggtcggcgat ctgcgcgccg ttccgtccgc cgaggaaccc
     1261 ggtcgcgacg accggcacgc cgaggtggcg gagcgcccgg gcgacgttca cgcccttccc
     1321 gcccgcgctc gagagcgcga ggctcgcgcg gtgccgctgc ccgagctgga agttcgggac
     1381 gagcagcgtc cggtccatgg ccgtgttgag ggtgacggtg acgatcacag gatcgctccg
     1441 agggcggcgt cgagcatgtc cccggcctct tccagcgtcc gcccagcgta ccaccccgcc
     1501 cgttcgagaa gccccggctc ttcgaaattc gaagcggcga cgagcggcct ccgcgcgacg
     1561 acccgctcgc cgtcgcggac gaccacctcg ccgaggacgt cgccctcacg gacgggcagg
     1621 tcgaccgcgg tctcggcgac caccgtctcg acgagcgggc gcccgaggag gagcaccggc
     1681 gcggcgctct tctccgccac gaggtcgacc cgctcgtcgg tgtacgggag gtctgcggag
     1741 gcgtacgcgc gtcccgtggt gacgacctcc gtgcgggcaa aacggtcgaa gccccagtcg
     1801 agcaggtcgg cgagcgcgtc gtttcgccgc gcgcgcgtcg gtgccccgag cacgaccgcg
     1861 tagacggtcg ccccgtcgcg gcgagcgacg ccgacctggc accagccggc gaggtccgtg
     1921 tgacccgtct tcacgccgaa gagcccgcga tactcgccga ggaggtcgtt ccagccgaag
     1981 agctcgcggc cgccggcgat cgcaagcgag tcctgccgga cgagccggcg gacgagcggc
     2041 tgctccatcg ccgcgcgggc gagcacgagc acgtcgcggg cggtcgaccg gtggcccggg
     2101 acgtcgagcc cgtccgggcg gacaaaggtc gtgtcgtcga gccccagctc ccgcgccttc
     2161 tcgttcatga gctcgacgaa ccgcgggacg ctgccgtcgc ccacgtgcgc ggcgagcgcg
     2221 aaggcggcgt cgttcgcgct ctggatgagc gcggccgcca ggaggtcgcg gacgctcatc
     2281 cgctcgccgg ggacgaggtc gatcgtcgac
//
LOCUS       DASTFC010000160         7279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_118294,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000160 DASTFC010000000
VERSION     DASTFC010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118294"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..249)
                     /locus_tag="VFO88_04800"
     CDS             complement(115..249)
                     /locus_tag="VFO88_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544872.1"
                     /translation="MDDEPRIDWDLEDALIQLLMRIDAKLDVLIELFEDEDGEEETDL
                     "
     gene            complement(299..919)
                     /locus_tag="VFO88_04805"
     CDS             complement(299..919)
                     /locus_tag="VFO88_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544873.1"
                     /translation="MPYRLQPVTGASIGGAPPPCVDCVFWQARPNRTTDKRRWAHRVE
                     DDWGAWGTLYVGDDDRLLGFVQAGPAHFFQRARVVPAGPPSGDAVLITCAYLVDASAP
                     WVMQSLFLSVIGEARDRGVKAIETFGYRYPEGESAYERFFVHRTIFPSDFLADFGFTP
                     IRWSGRVALARLELAGLQPVEEGAPARVLRHVKEAFTPAPPVPQRP"
     gene            complement(944..1693)
                     /locus_tag="VFO88_04810"
     CDS             complement(944..1693)
                     /locus_tag="VFO88_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9544874.1"
                     /translation="MELRRVPVPLPGLPRALDGLRVLHLSDLHLGTISLGARALGKAV
                     AWADEERPDLVAITGDLVTRARGRRALEDALGRLQPRLGTFAVLGNHDVDDSRDPFRR
                     PADLSDLAVPGTRLLRDDEAVVEVGGARVQIVGVDPSSYRRGGSRPAARADRDADLRI
                     LLCHYPDIVRRVPEGLFELVLAGHFHGGQLAVPTPWGRIRLKDLRATYWEGLFDTPAG
                     ALHVSRGLGTSFVPFRLWARPEATLLTLGRA"
     gene            complement(1771..2655)
                     /locus_tag="VFO88_04815"
     CDS             complement(1771..2655)
                     /locus_tag="VFO88_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HET9544875.1"
                     /translation="MRVAYYKGCLASLSAKELDTSTQALAPKLGIELDELESVTCCGA
                     GDIHEAEPDYYLHLNARILAYAEATGSDTLMTICNVCTLNLRQANHMLQNDEALMKRV
                     SDNLESVGAPPYSGGVEVKHFLWLIAAGEGYDRLKSVAHKGLKGLKVAPFYGCQILRP
                     SKLLGFEDPDRPSSLEAIIEACGGEAIDYPAKIKCCGFPIIQAREETALNELILPIEQ
                     SMEAGADAMVTPCPLCHLSLDAWQQKLEASTGRSFGMPILHLSQLIGVAAGLEDSELR
                     FRRHVVSMDPVLEKVEAP"
     gene            complement(2652..3764)
                     /locus_tag="VFO88_04820"
     CDS             complement(2652..3764)
                     /locus_tag="VFO88_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET9544876.1"
                     /translation="MATELQTNGGAVTLGAMDVVLKIWRSDAETGERSLERYEVTVGE
                     EATLLDCLDVVKDKVDGTLAYRKSCRMMICGSCGMRLDGRAVLACKERMKPYVDRGHV
                     PVVSPMGNLPVVRDLVVDMGPFWSKIRAVKPWLDTGYQEHPEGEFRHSQQAMNPIHKE
                     SLCIMCGCCVSECNSMEADPEFLGPAALAKGFRFVGDPRDRGKIERLEALNEEHGIWD
                     CTRCYFCNERCPKGVDPRDAIAKLGAESIEAGIDRDMGAKHAKWFVRSAETTGWLRET
                     ELVPKTQGLAEAIRQTKFAVSLAKRGKVPLPFPPHVAKDVREARMLRELVRSQGRTGA
                     AGHIQGERALSRIEHVLHDDPEKAPASRIAPEKARG"
     gene            complement(3815..4651)
                     /locus_tag="VFO88_04825"
     CDS             complement(3815..4651)
                     /locus_tag="VFO88_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544877.1"
                     /translation="MRRLGGLAAAVSLAAVLAACGGDGNGSGEEADAPAGGDAATPVA
                     VAAAETAQAESYRSSIAIEYESSQGAVRIAGEGEFQAEPPRGRLALNVAEAGGRADVA
                     GTEIVYDGQVLYMAAPGAAEGPERRWVAVDLGEGGRLGQFGGFDRADPAQALAYLRIA
                     DDAEEVGTEEVRGEETTRYRLTIDLREAAERSPEYRSVIEQSLSTGGSAEVPTDVWVD
                     DEGRVRRVRMVYADVTTGEGTTADVTVTTELYDFGAEVEIEPPPRDQVLNLGEDGGVG
                     TS"
     gene            complement(4651..6351)
                     /locus_tag="VFO88_04830"
     CDS             complement(4651..6351)
                     /locus_tag="VFO88_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9544878.1"
                     /translation="MEAQHDVLVVGAGCAGMRAAIEAHDAGADVGVISKLHPTRSHSG
                     AAEGGINAALGNTGDDSPETHAFDTVKGSDYLGDQDAIEIFTREAPHDIYQLEHWGAV
                     FSRNDDGKLAQRPFGAAGSPRTVFAADITGHVLIHVLYEQLMKRVAAGPMHVYEEFFA
                     WRLVVDGGRCVGVICWDILNGGLKLLTGKTVVLATGGAGRLYRATTNAYACTGDGMAM
                     ALRAGLPLKDMEFMQFHPTTLYPSGILITEGCRGEGAYLVNNEGERFMQRYAPNAMEL
                     ASRDVVSRSEQTEIDEGRGVNGSIFLDMRHLGAERILERLPGSRELAMTYVGVDPIHE
                     PIPVRPGAHYHMGGVETDEWGKTALDGLYAAGEVACVSVHGANRLGGNSLMETITFGR
                     RSGRHAAEAAHGAGGAPSAPESALRDAERDLQALLDVRDGERPWQIRDELGASMLENF
                     GVFRREDQMARQIEIIRGLRERYDRGIVVEDKGRVFNSDLTQAIELGYLLDLAEVMLQ
                     AGIARKESRGAHSRPHDYPNRDDEQFLKHSISTWNGGGPELSYREVRMTRWQPTERTY
                     "
     gene            complement(6401..>7279)
                     /locus_tag="VFO88_04835"
     CDS             complement(6401..>7279)
                     /locus_tag="VFO88_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET9544879.1"
                     /translation="GKTTLSADPERRLIGDDEHGWGGAGVFNFEGGCYAKMIRLSPEA
                     EPEIFRTTHTFGTVLENVVMDERGTLDLDDDSKTENTRGAYKLEQIANALPEKRAGHP
                     RTVVFLTADAFGILPPIARLSPEQAMFYFLSGFTAKLAGTEIGVKEPQPTFSACFGAP
                     FLPQHPAVYARMLGEKLDEHGSTVWLVNTGWTGGPYGEGERMPIAATRALLAAALSGE
                     LDDAEYREDPVFGFQVPVSVSGVDDRLLDPRGTWRHGADYDRKAAELAGLFRENFAKF
                     AGAAGDAVAAAGPRVA"
BASE COUNT          968 a         2712 c         2541 g         1058 t
ORIGIN      
        1 cggcctagct acgccgccga gctacgcccg cgacggcgcg cctcgaggcg cgtcacgagc
       61 tcgcggagat cccgctggat ctcgcggagc tcggccgcca gccgccgccg ctggtcagag
      121 gtcagtttct tcttcgccat cctcgtcctc gaagagctcg atcaacacat cgagcttagc
      181 gtcgatccgc atcagcaact gaatcagcgc gtcctcgaga tcccagtcga tgcgcggttc
      241 gtcgtccacc gggccatcgt gctgaagctg actggtcgcc gcaacggccg acagggccct
      301 acggcctctg cggcacaggt ggagcggggg tgaacgcctc cttgacgtgg cgcaggacgc
      361 gggcgggcgc gccctcctcg accggctgga ggccggcgag ctcgagccgc gcgagcgcca
      421 cccgccccga ccagcggatc ggcgtgaacc cgaagtcggc gaggaagtcg ctcgggaaga
      481 tcgtgcggtg gacgaagaac cgctcgtagg cgctctcgcc ctccgggtag cggtagccga
      541 acgtctcgat cgccttgacg ccacggtcgc gcgcctcgcc gatcactgag aggaacaggc
      601 tctgcatcac ccacggggcc gacgcgtcga cgaggtacgc gcacgtgatc agcaccgcgt
      661 cgcccgacgg cggcccggcc ggcaccacgc gggcgcgctg gaagaagtgc gccggcccgg
      721 cctggacgaa cccgagcagc cggtcgtcgt cgcctacgta gagcgtcccc cacgcgcccc
      781 agtcgtcctc gacccggtgc gcccagcgcc gcttgtccgt cgtccggttc ggccgcgcct
      841 gccagaagac gcagtcgacg cagggcggcg gcgccccgcc gatcgaggcg ccggtcacgg
      901 gctggagccg gtagggcatg accgtatctt ggcgaaccgg cgcctacgcg cggccgagcg
      961 tgaggagcgt ggcctccggc ctcgcccaga gccggaacgg cacgaagctc gtcccgagcc
     1021 cccgggagac gtgcaacgcg cccgccggcg tgtcgaagag gccctcccaa tacgtcgccc
     1081 tcaagtcctt caggcggatt cgcccccacg gcgtgggcac ggcgagctgg ccgccgtgga
     1141 agtgccccgc gagcacgagc tcgaagaggc cttccggcac ccgccggacg atgtccgggt
     1201 agtggcagag caggatgcgc agatcggcgt cccggtcggc gcgcgccgcc gggcgcgatc
     1261 cgccccgccg gtacgacgag gggtcgacgc cgacgatctg cacccgcgcg cccccgacct
     1321 ccaccaccgc ctcgtcgtcg cgcaggagcc gcgtccccgg cacggcgagg tcagagaggt
     1381 cggccggccg gcggaacggg tcgcgcgagt cgtccacgtc gtggttcccg agcaccgcga
     1441 acgtcccgag ccgcggctgc agccgcccga gcgcgtcctc gagggcccgg cgcccccgcg
     1501 cgcgcgtgac gagatccccc gtgatcgcta cgagatcggg ccgctcctcg tccgcccagg
     1561 cgaccgcctt cccgagcgcc cgcgcgccga gcgagatcgt cccgaggtga agatccgaga
     1621 ggtgcagcac gcggaggccg tcgagcgctc tcggaagccc gggcagcgga accggcacgc
     1681 gccggagctc cacccactgc gactcgaaga gcgaccacgc cgcggccccg gccccgaggc
     1741 cggccgccgc gccgagccac cggccacggg ctacggggct tcgaccttct ccagcacggg
     1801 gtccatcgag acgacgtggc gcctgaagcg gagctccgag tcctcgagac cggccgcgac
     1861 gccgatcagc tgcgagaggt ggaggatcgg catcccgaac gagcgcccgg tcgacgcctc
     1921 gagcttctgc tgccacgcgt cgagcgagag gtggcagagc gggcacggcg tcaccatcgc
     1981 gtccgcgccg gcctccatcg actgctcgat cggcaggatg agctcgttca gtgcggtctc
     2041 ctcgcgagcc tggatgatcg ggaacccgca gcacttgatc ttcgccgggt agtcgatcgc
     2101 ctcgccgccg caggcctcga tgatcgcctc gagcgacgac ggacggtccg ggtcctcgaa
     2161 cccgaggagc ttggacggcc gcaggatctg gcacccgtag aacggcgcca ccttgaggcc
     2221 cttgagcccc ttgtgggcga ccgacttcag ccggtcgtag ccctcgccgg ccgcgatgag
     2281 ccagaggaag tgcttcacct cgaccccgcc cgagtagggc ggcgcgccga ccgactcgag
     2341 gttgtcgctc acgcgcttca tgagcgcctc gtcgttctgg agcatgtggt tcgcctgccg
     2401 caggttcagc gtgcagacgt tgcagatcgt catgagcgtg tccgagccgg tcgcctccgc
     2461 gtacgcgagg atccgcgcgt tcaggtggag gtagtagtcc ggctccgcct cgtggatgtc
     2521 cccggcgccg cagcacgtga ccgactcgag ctcgtcgagc tcgatcccga gcttcggcgc
     2581 gagcgcctgc gtggacgtgt cgagctcctt tgccgagagc gacgcgaggc agcccttgta
     2641 gtaggcgacc ctcatccgcg tgccttctcc ggcgcgatcc ggctcgcggg cgccttctcc
     2701 gggtcgtcgt gcaggacgtg ctcgatgcgg gagagcgcgc gctcgccctg gatgtggccg
     2761 gccgcgcccg tgcggccctg cgagcgcacg agctcgcgga gcatgcgcgc ctcgcggacg
     2821 tccttcgcga cgtgcggcgg gaagggcagc ggcaccttgc cgcgcttggc gagggagacc
     2881 gcgaacttcg tctgcctgat cgcctcggcg agcccctgcg tcttcggcac gagctcggtc
     2941 tcgcggagcc agccggtcgt ctcggcggag cggacgaacc acttcgcgtg cttcgcgccc
     3001 atgtcgcggt cgatgccggc ctcgatcgac tcggcgccga gcttcgcgat cgcgtcgcgc
     3061 gggtcgacgc ccttcgggca gcgctcgttg cagaagtagc accgggtgca gtcccagatc
     3121 ccgtgctcct cgttcagggc ctcgagccgc tcgatcttcc cccggtcgcg cgggtcgccc
     3181 acgaagcgga agcccttcgc gagcgccgcg ggccccagga actccgggtc ggcctccatc
     3241 gagttgcact ccgagacgca gcagccgcac atgatgcaga gcgactcctt gtggatcggg
     3301 ttcatcgcct gctgcgagtg gcggaactcg ccctcgggat gctcctggta gcccgtgtcg
     3361 agccacggct tgacggcgcg gatcttgctc cagaacggcc ccatgtcgac gacgaggtcg
     3421 cggacgacgg ggaggttccc catcggcgag acgaccggga cgtggccgcg gtcgacgtag
     3481 ggcttcatgc gctccttgca cgcgagcacg gcgcggccgt cgagccgcat cccgcaggag
     3541 ccgcagatca tcatccggca gctcttgcgg tacgcgagcg tgccgtccac cttgtccttc
     3601 accacgtcga ggcagtcgag gagcgtcgcc tcctcgccca ccgtgacctc gtagcgctcg
     3661 aggctgcgct cgccggtctc cgcgtcggag cgccagatct tcaggacgac gtccatcgcc
     3721 ccgagcgtaa ccgccccgcc gttcgtctgc agttccgtcg ccatgggcct cctagtcggt
     3781 ctcctgcaac gtatccgtcc tgcccgtcgc cacgctacga cgtccctacc ccgccgtctt
     3841 cgccgaggtt caggacctga tcgcgcggcg gcggctcgat ctcgacctcg gcgccgaagt
     3901 cgtagagctc ggtcgtcacc gtcacgtccg cggtcgtgcc ctcgccggtc gtgacgtcgg
     3961 cgtagaccat gcgcacgcgc cgcacgcgcc cctcgtcgtc cacccagacg tcggtcggca
     4021 cctcggccga gccgccggtc gagaggctct gctcgatcac ggagcggtac tccggcgagc
     4081 gctccgcggc ctcgcgaagg tcgatcgtga gccggtagcg cgtcgtctcc tcgccgcgca
     4141 cctcctccgt cccgacctcc tccgcgtcgt cggcgatgcg gagatacgcg agcgcctggg
     4201 ccggatctgc gcggtcgaag ccgccgaact ggccgagacg gccgccctcg ccgaggtcga
     4261 ccgcgaccca ccgccgctcg ggcccctcgg cggccccggg cgcggccatg tagaggacct
     4321 ggccgtcgta gacgatttcc gtgcccgcca cgtcggcgcg cccgcccgcc tcggcgacgt
     4381 tcagcgcgag gcggccgcgc gggggctcgg cctggaactc cccctcgccg gcgatccgca
     4441 cggccccctg cgagctctcg tactcgatcg cgatgctcga gcggtagctc tccgcctgcg
     4501 cggtctccgc cgccgcgacc gcgaccgggg tcgcggcgtc ccctccggcc ggcgcgtccg
     4561 cctcctcgcc gctcccgttc ccgtcgccgc cgcaggccgc gaggacggcg gcgagcgaga
     4621 ccgccgccgc gagccccccg agcctgcgca tcagtacgtc cgctccgtcg gctgccagcg
     4681 ggtcatgcgc acctcccggt aggagagctc gggcccgccg ccgttccagg tcgagatcga
     4741 gtgcttgagg aactgctcgt cgtcgcggtt cgggtagtcg tgcgggcggg agtgcgcgcc
     4801 gcggctctcc ttgcgcgcga tccccgcctg gagcatgacc tcggcgagat cgagcaggta
     4861 cccgagctcg atcgcctgcg tgaggtccga gttgaagacg cgccccttgt cctcgacgac
     4921 gatgccgcgg tcgtaccgct cgcgcaggcc gcggatgatc tcgatctgcc gcgccatctg
     4981 atcctcgcgg cggaagacgc cgaagttctc gagcatcgag gcgccgagct cgtcgcggat
     5041 ctgccacggc cgctcgccgt cgcggacgtc gaggagcgcc tggaggtcgc gctcggcgtc
     5101 gcgcagcgcc gactcgggcg cggacggcgc gccgccggcc ccgtgcgccg cctcggccgc
     5161 gtgccgcccc gagcgccggc cgaaggtgat cgtctccatg agcgagttcc cgccgagccg
     5221 gttcgcgccg tggacggaga cgcatgccac ctcgccggcc gcgtagaggc cgtcgagcgc
     5281 cgtcttgccc cactcgtccg tctcgacccc gcccatgtgg tagtgcgctc cgggccggac
     5341 ggggatcggc tcgtggatcg ggtcgacgcc cacgtacgtc atcgcgagct cccgcgagcc
     5401 gggcagccgc tcgaggatcc gctccgcgcc gaggtggcgc atgtcgagga agatcgagcc
     5461 gttcacgccg cgcccctcgt cgatctccgt ctgctccgag cgggagacga cgtcgcgcga
     5521 ggcgagctcc atcgcgttcg gcgcgtagcg ctgcatgaag cgctcgccct cgttgttgac
     5581 gaggtacgcg ccctcgccgc ggcagccctc ggtgatgagg atcccgctcg ggtagagcgt
     5641 cgtcgggtgg aactgcatga actccatgtc cttcaacggg agcccggccc tgagggccat
     5701 cgccatcccg tcgccggtgc aggcgtacgc gttcgtcgtc gcccggtaga gccggccggc
     5761 cccgcccgtc gcgaggacga ccgtcttgcc ggtcaggagc ttcaggccgc cgttcaggat
     5821 gtcccagcag atgacgccga cgcagcggcc gccgtcgacg acgagccgcc acgcgaagaa
     5881 ctcctcgtag acgtgcatcg gccccgcggc gacgcgcttc atgagctgct cgtagagcac
     5941 gtggatcagc acgtggcccg tgatgtcggc cgcgaagacc gtgcgcggcg acccggccgc
     6001 gccgaacggg cgctgcgcga gcttgccgtc gtcgtttcgc gagaagaccg cgccccagtg
     6061 ctcgagctgg tagatgtcgt gcggcgcctc gcgcgtgaag atctcgatcg cgtcctggtc
     6121 gccgaggtag tccgagccct tgaccgtgtc gaacgcatgc gtctcggggc tgtcgtcgcc
     6181 cgtgttgccg agcgccgcgt tgatcccgcc ctcggccgcg ccggagtggc tgcgcgtggg
     6241 atggagcttc gagatgacgc cgacgtccgc gcccgcgtcg tgcgcttcga tcgcggcccg
     6301 catgccggcg cagccggcgc cgacgacgag gacgtcgtgc tgtgcctcca cgggtgcgaa
     6361 cgctatacga ccggacgccg ccggagcctc gtatccggcg ctacgcgacg cgcgggccgg
     6421 cggccgcgac ggcgtcgccc gccgcgccgg cgaacttggc gaagttctcc cggaagagcc
     6481 cggccagctc ggccgccttg cggtcgtagt cggcgccgtg gcgccacgtc ccgcgcgggt
     6541 cgagcaggcg gtcgtcgacg ccggagacgg acacgggcac ctggaagccg aagacgggat
     6601 cctcgcggta ctcggcgtcg tccagctcgc ccgagagcgc cgccgcgagg agcgcgcgcg
     6661 tggccgcgat cggcatccgc tcgccctcgc cgtagggccc gcccgtccac ccggtgttca
     6721 cgagccagac cgtcgagccg tgctcgtcga gcttctcgcc gagcatccgc gcgtacacgg
     6781 ccggatgctg cggcaggaac ggcgccccga agcaggcgga gaacgtcggc tgcggctcct
     6841 tcacgccgat ctccgtcccg gcgagcttcg cggtgaagcc ggagaggaag tagaacatcg
     6901 cctgctcggg cgagagccgc gcgatcggcg ggaggatccc gaacgcgtcc gcggtcagga
     6961 agacgaccgt gcgcgggtgc ccggcgcgct tctccgggag cgcgttcgcg atctgctcga
     7021 gcttgtacgc gccgcgcgtg ttctccgtct tcgagtcgtc gtcgaggtcg agcgtgccgc
     7081 gctcgtccat gacgacgttc tcgagcaccg tcccgaaggt gtgcgtcgtg cggaagatct
     7141 ccggctcggc ctcgggcgag aggcggatca tcttcgcgta gcagccgccc tcgaagttga
     7201 agacgccggc cccgccccac ccgtgctcgt cgtcgccgat caggcggcgc tccgggtcgg
     7261 ccgagagcgt cgtcttccc
//
LOCUS       DASTFC010000161         8510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_727892,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000161 DASTFC010000000
VERSION     DASTFC010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727892"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1353)
                     /gene="tyrS"
                     /locus_tag="VFO88_04840"
     CDS             complement(178..1353)
                     /gene="tyrS"
                     /locus_tag="VFO88_04840"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9544880.1"
                     /translation="MADPALVRELTRNAVHVLPEGELERKLGRGRPLRVKLGIDVTSP
                     DIHVGRAIPLQRMRAFQDAGHLGVLIVGDYTTRIGDPSGRSSERPVLSDEEIDRNAQT
                     YVDQAMIVLDPDRTEVRYNGEWLAKLDFAEIVRLTRVATVAELLTRDDFRKRYESELP
                     ISVSELLYPLAQGYDSVAVEADVELGGTDQLYNLLMGRKVMEAYGLEPQVALTTPLLL
                     SWDGEKMSSSIGNNVPLTAPPEEQFGRTMRIPDELLPEWYRLVLESDDDPLAGDPMGA
                     KLRLARWVVARSHGEEAAEAAEAHFTRVVREGRAPEEVPEVTVAADGGTVYLPAVLVD
                     LGVASTSEGRRLIAQGGVRLDGEAVTELEVPASRLDGALLQAGKRRFARVSVRPETA"
     gene            1410..3833
                     /locus_tag="VFO88_04845"
     CDS             1410..3833
                     /locus_tag="VFO88_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET9544881.1"
                     /translation="MRFRRRERKQRTRRVRKLRLLALVGVLGLVCSVSFTYGLVTAIS
                     SEIPALDPSNQTQLAEDGFIYASDGKTVLARLRGEESRVVVPSDQIANDMKRAIVAVE
                     DRRFWEHRGIDLRGIARALWADIRHKELVEGGSTITQQFVKNTYTRDDRTISRKLKEA
                     ALAWQLEQRWSKDRILTAYLNTVFFGNGAYGVQMAARVYFGKKARDLTLAESALLAGI
                     PANPSAYDPVLNPRAAKERRGVVLQLMRDQNLITEQELRAANETPMPSPDSVSLPESR
                     GPAGYFAEYVKQQLIDHYGSGEVFGGGLKVTTSIDIELQQLARRAVDEWLGEANGPSA
                     ALVAVDPRDGRVLAMYGGSSFEESQFNLAVQGERQSGSAFKPFVLAAALESGISPQTT
                     FQSQPTTINLGDRSWTVGNYEGTYLGTIDLVKATASSDNAVYAQLTSLVGPRKVRETA
                     HALGITSPLNDYFAIGLGVEAVNPLEMARAFATFANGGARVDGRVLGNRPRAVLSVDR
                     GRRTEENTPVGQQVLDPENNATLTWMLQQVVEDGTGERAAIPGRAVAGKTGTTENYGD
                     AWFVGYTPQLAVAVWVGYPKNLKPMETEFEGGPVAGGTYPALIWNTFAESALRHMGEP
                     PASFPAPAYKAATAVEVAYRDQEWRLDNGYCSNTRLVLYVAGYEPDEQADCKPNEVDV
                     PRVVGQTVEAAEAQLASMPLTAEVITRPARPGERIGVVVDQYPKGGTLSSWDVVRIVT
                     SEPSGPVVPRLVGMKLPRAQGVLEELGLAPFVELVTEGRKGVVLAQRPRAGIHVPDGA
                     RIRLRVGRG"
     gene            complement(3808..4389)
                     /locus_tag="VFO88_04850"
     CDS             complement(3808..4389)
                     /locus_tag="VFO88_04850"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET9544882.1"
                     /translation="MTEPRPLTRDFFARSVHEVAPELVGARLLVDGVGGTIVEVEAYD
                     GEDPASHGFRGETARNRSMFGPPGHAYVYRSYGIHWCLNLVCAEPGRAEAVLVRALEP
                     TDGLDAMRKRRGGQPDDRLLCAGPGRLCQALGVTAAHDGLPLDAPPFELEAAPEPAAV
                     VRGPRVGLTKAVDEPWRYALAGSRYLSRALRAA"
     gene            complement(4386..4745)
                     /locus_tag="VFO88_04855"
     CDS             complement(4386..4745)
                     /locus_tag="VFO88_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544883.1"
                     /translation="MADGQPRMVFLGFGKYARADKIYALEPLTGKERGGGHRTKVWVE
                     GIPDPIVASRTERTIRHAMGASASDTSALVDEAVTLAERIVDDAERGRVDLGDLARRA
                     RHLLEATARPAESDQLF"
     gene            4808..5296
                     /locus_tag="VFO88_04860"
     CDS             4808..5296
                     /locus_tag="VFO88_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544884.1"
                     /translation="MEGESERPAEGRSGSWIGWELLRVLQDGLVSLELTASRVLAAQV
                     AGLITLWTQLHTFEDGPPAGLAWLALLLFMGGIATLGALLRPRRVTRFWDRALPADLF
                     SAERVMAAGEETATLAHVATAIRGQRDALERALRVSVPLGLSGLAMTALAYALEKAFY
                     AP"
     gene            complement(5625..6560)
                     /locus_tag="VFO88_04865"
     CDS             complement(5625..6560)
                     /locus_tag="VFO88_04865"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9544885.1"
                     /translation="MRETEKIWMNGELVDWADAKIHVGAHGLHYGSGVFEGIRCYETE
                     KGSAVFRLTDHLQRLHNSARLIQMELPFSVEELKAACNEVIGVNGLPECYLRPLAFYG
                     YGELGVRPTGCPVDVVIMSWPWGTYLGDDALENGVRAKISSWQRIGPNVIPHVAKATG
                     VYLNSMLAVLEASKAGYEEAILLTETGYVADGSGENIFIVKNGRIATPDLSSSILPGI
                     TRDSVIQIAQDLGYTVEEKNLVRADLYLADEIFMCGTAAEVTPVRAVDDREVGDPGPI
                     TREIQSAYLDTVRGKREQWSHWLEYAPNPSAVTGS"
     gene            complement(6571..7011)
                     /locus_tag="VFO88_04870"
     CDS             complement(6571..7011)
                     /locus_tag="VFO88_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HET9544886.1"
                     /translation="MAIERRAEVVWEGPLASGSGEAKGASSNAFSLPVTWSARTEAAD
                     GMTSPEELIAAAHASCYAMAFSNVLAEAGSPPDRLEVAAVVSAELDDDGLRVTTSELT
                     VTGRVPGLEASDFSRHAQDAERACPVSNALRGNVEIRVDARLED"
     gene            complement(7023..7520)
                     /locus_tag="VFO88_04875"
     CDS             complement(7023..7520)
                     /locus_tag="VFO88_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET9544887.1"
                     /translation="MAGDWPEPVERVAAVLRSNGIEGRVEEFRSGTPTAEAAAEAAGC
                     GLEQIVKSLLFALDGGERYALVMVPGDRRADRAKIAAELGVGKAKPASPQEVLDVTGF
                     DVGGVAPFPAGRVEASLLDRTLVTHEVVWVGAGSPRHMAVLAPADLARLTRARVVDAV
                     SDTTT"
     gene            complement(7521..>8510)
                     /locus_tag="VFO88_04880"
     CDS             complement(7521..>8510)
                     /locus_tag="VFO88_04880"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9544888.1"
                     /translation="ATLTEESRGMIGAEELTKAREGLRLVNVARGELVVEDALVEALR
                     DGRVAGAALDVFAAEPYTGPLAEFENVILTPHLGASTAEAQDRAGVIVAEQVAAALEG
                     SVVTNAVNMPAVGAEDFAVLGPFLPLVADLAALAVALAGGSPNRLELVYLGRLAERDT
                     RLLTAAALNGVFQGRVEQAVNFVNAPLIARSRGLEVVESSSASSPDFTSLVTVTAYDA
                     DGRAYAAHGTTIGQENRRQLTRVLDYEIDVELDKQMLFAENDDTPGMIGRIGTILGES
                     GINIANMAVSRNRHERRALMVLTLDDVPSEDVLERLRAEPGFVEARFIVLPEP"
BASE COUNT         1157 a         3049 c         3104 g         1200 t
ORIGIN      
        1 agaccatgcc tctcggcccg gcgcaaagaa agagcctccg ccgcgtggag gcccctcaag
       61 agactgcttc cgacgtatcg ggggctgctc taaggggctt ctccgttcgc tcgttgacct
      121 tttccatgcg ccgccccgta gagcggccga cgcatgatag caagggcggg gccgtcgtca
      181 agcggtttcg gggcggacgg agacccgcgc gaagcggcgc tttccggcct gcaggagcgc
      241 cccgtcgagg cggctcgccg gcacctccag ctcggtgacc gcctccccgt cgagcctgac
      301 cccgccctgg gcgatcaggc gccgcccctc gctggtcgac gcaacgccga gatcgacgag
      361 cacggccggc aggtagacgg tcccgccgtc ggccgcgacg gtcacctccg gcacctcctc
      421 cggcgcgcgg ccctcgcgca cgacgcgcgt gaaatgcgcc tcggcggcct ccgcggcctc
      481 ctccccgtgc gagcgcgcga ccacccaccg cgcgaggcgg agcttcgcgc ccatcgggtc
      541 gccggcgagc gggtcgtcgt ccgactcgag gacgagccgg taccactcgg gcaggagctc
      601 gtccgggatc cgcatcgtgc gcccgaactg ctcctcgggc ggcgccgtga gcggcacgtt
      661 gttcccgatc gacgagctca tcttctcgcc gtcccaggag aggaggagcg gcgtcgtgag
      721 cgcgacctgc ggctcgaggc cgtacgcctc catcaccttg cggcccatga ggaggttgta
      781 gagctggtcg gtgccgccga gctccacgtc ggcctcgacg gcgaccgagt cgtagccctg
      841 cgcgagcggg tagaggagct cggagaccga gatcgggagc tccgactcgt agcgcttgcg
      901 gaagtcgtcg cgcgtcagga gctcggcgac cgtggcgacg cgcgtgagcc ggacgatctc
      961 cgcgaagtcg agcttcgcga gccactcgcc gttgtacctg acctccgtcc ggtcggggtc
     1021 gaggacgatc atcgcctggt cgacgtacgt ctgcgcgttg cggtcgatct cctcgtccga
     1081 gagcaccggc cgctcgctcg agcgcccgga ggggtcgccg atccgcgtcg tgtagtcccc
     1141 gacgatgagc acgccgaggt gccccgcgtc ctggaaggcg cgcatccgct ggagcgggat
     1201 cgcccgcccc acgtggatgt cgggcgacgt gacgtcgatg ccgagcttca cgcgcagcgg
     1261 gcggccgcgg ccgagcttgc gctcgagctc gccctcgggg aggacgtgca cggcgttccg
     1321 ggtcagctcg cggacgagcg cgggatcggc catggggcga tcgtagtccg caggccccgc
     1381 cggtgcgggc gactagaatc gtctgcttcg tgcgcttccg acgtcgtgag aggaagcagc
     1441 ggacccgccg ggtccgcaag cttcggctcc tcgctctggt cggggtcctc gggctcgtct
     1501 gctccgtctc gttcacgtac gggctcgtca ccgcgatctc gagcgagatc cctgcgctgg
     1561 acccgtccaa ccagacgcag ctcgcggagg acgggttcat ctacgcctcg gacggcaaga
     1621 cggtcctcgc gcgcctgcgc ggcgaggaga gccgcgtcgt cgtcccatcc gaccagatcg
     1681 cgaacgacat gaagcgcgcg atcgtcgccg tcgaggacag gcgcttctgg gagcaccgcg
     1741 ggatcgacct gcgcgggatc gcgcgcgcgc tctgggcgga catccgccac aaggagctcg
     1801 tcgagggcgg ctcgacgatc acgcagcagt tcgtgaagaa cacgtacacg cgcgacgacc
     1861 gcacgatcag ccgcaagctc aaggaggcgg cgctcgcgtg gcagctggag cagcgctggt
     1921 cgaaggaccg gatcctcacc gcctacctga acaccgtctt cttcgggaac ggcgcgtacg
     1981 gcgtgcagat ggcggcgcgc gtctacttcg ggaagaaggc gcgcgacctg acgctcgccg
     2041 agtcggcgct cctcgccggg atcccggcga acccgagcgc gtacgacccc gtcctgaacc
     2101 cgcgggcggc gaaggagcgg cgcggcgtcg tcctccagct catgcgcgac cagaacctca
     2161 tcaccgagca ggagctgcgc gcggcgaacg agacgccgat gccgtcgccc gactcggtca
     2221 gcctccccga gtcgcgcggg cccgcgggct acttcgccga gtacgtgaag cagcagctca
     2281 tcgaccacta cggctccggc gaggtcttcg gcggtgggct caaggtcacg acctcgatcg
     2341 acatcgagct ccagcagctc gcgcggcgcg cggtggacga gtggctcggc gaggcgaacg
     2401 ggccgtcggc ggcgctcgtc gcggtcgacc cgcgcgacgg gcgcgtgctc gcgatgtacg
     2461 ggggctcgag cttcgaggag agccagttca acctcgcggt gcagggcgag cggcagtccg
     2521 gctccgcgtt caaaccgttc gtgctcgcgg ccgcgctcga gtcggggatc tcgccgcaga
     2581 cgacgttcca gtcgcagccg acgacgatca acctcgggga ccgttcctgg accgtcggga
     2641 actacgaggg gacgtacctc gggacgatcg acctcgtgaa ggcgacggcc tcgtcggaca
     2701 acgcggtgta cgcgcagctc acgtcgctcg tcgggccgcg gaaggtgcgc gagaccgcgc
     2761 acgcgctcgg gatcacgagc ccgctgaacg actacttcgc gatcgggctc ggcgtcgagg
     2821 cggtgaaccc gctcgagatg gcgcgcgcgt tcgcgacgtt cgcgaacggc ggcgcacgcg
     2881 ttgacgggcg cgtgctcggg aaccggccgc gcgccgtcct ctcggtcgac cgcggccggc
     2941 ggacggagga gaacacgccg gtcggccagc aggtgctcga ccccgagaac aacgcgacgc
     3001 tcacgtggat gctccagcag gtggtcgagg acgggaccgg cgagcgggct gcgatcccgg
     3061 gccgggcggt cgccgggaag acggggacga cggagaacta cggcgatgcc tggttcgtcg
     3121 gctacacgcc gcagctcgcg gtcgcggtct gggtcgggta tccgaagaac ctgaagccga
     3181 tggagacgga gttcgagggc gggccggtcg cgggcggcac gtacccggcg ctcatctgga
     3241 acaccttcgc cgagagcgcg ctccggcaca tgggcgagcc gccggcgagc ttccccgcgc
     3301 ccgcgtacaa ggcggcgacg gctgtcgagg tggcctaccg cgaccaggag tggcgcctgg
     3361 acaacggcta ctgctcgaac acgcggctcg tcctctacgt ggccggctac gagccggacg
     3421 agcaggccga ctgcaagccg aacgaggtgg acgtcccccg cgtcgtcggg cagaccgtcg
     3481 aggcggcgga ggcgcagctc gcgagcatgc cgctgacggc cgaggtgatc acgcgcccgg
     3541 cgcggcccgg ggagcgcatc ggcgtcgtcg tcgaccagta tccgaagggc ggcacgctct
     3601 cctcgtggga cgtcgtccgg atcgtgacgt ccgagccgag cgggccggtc gtgccgcggc
     3661 tcgtcggaat gaagctcccg cgcgcgcagg gggtgctcga ggagctcggc ctcgcgccgt
     3721 tcgtcgagct cgtgacggag gggcgcaagg gcgtcgtgct cgcgcagcgg ccgcgcgccg
     3781 gcatccacgt gcccgacggc gcgaggatca ggctgcgcgt agggcgcggc tgaggtagcg
     3841 cgagccggcg agcgcgtagc gccacggctc gtcgaccgcc ttcgtgaggc cgacccgcgg
     3901 cccgcggacg acggcggcgg gctcaggcgc ggcctcgagc tcgaacggcg gcgcgtcgag
     3961 cgggagcccg tcgtgcgccg cggtcacgcc gagcgcctgg cagaggcgcc cggggccggc
     4021 gcagaggagg cggtcgtccg gctgcccgcc ccgccgcttc cgcatcgcgt cgagcccgtc
     4081 cgtcggctcg agcgcgcgga cgaggacggc ctccgcgcgg cccggctcgg cgcagacgag
     4141 gttcaggcac cagtggatcc cgtacgagcg gtagacgtac gcgtggccgg gcgggccgaa
     4201 catcgagcgg ttccgtgccg tctcgccgcg gaacccgtgg ctcgccggat cctcgccgtc
     4261 gtacgcctcg acctcgacga tcgtcccgcc caccccgtcc acgagcaggc gcgcgccgac
     4321 gagctcgggc gcgacctcgt gcaccgagcg ggcgaagaag tcgcgcgtga gggggcgggg
     4381 ctcggtcaga agagctggtc cgactcggcc ggccgggcgg tcgcctcgag caggtggcgg
     4441 gcgcggcgtg cgaggtcgcc gaggtcgacg cggccgcgct cggcgtcgtc gacgatccgc
     4501 tcggcgagcg tgaccgcctc gtcgacgagc gcgctcgtgt cgctcgcgga cgcgcccatc
     4561 gcgtggcgga tcgtccgctc ggtccgggag gcgacgatcg ggtcggggat cccctcgacc
     4621 cagaccttcg tccggtggcc gccgccccgc tccttcccgg tgagcggctc gagcgcgtag
     4681 atcttgtccg cgcgcgcgta tttcccgaag cccaggaaga ccatccgcgg ctgcccgtcc
     4741 gccatgggcg gaagtatcgc aagccgggcc ggccggaagc gtccggtggg aggtctaccc
     4801 tcgtttcatg gagggcgagt ccgagcgccc tgccgagggg cgctccggga gctggatcgg
     4861 gtgggagctc ctgcgcgtcc tccaggacgg gctcgtctca ctcgagctga cggcctcccg
     4921 ggtcctcgcg gcgcaggtgg cggggctcat cacgctctgg acgcagctcc acaccttcga
     4981 ggacggcccg ccggcggggc tcgcgtggct cgcgctcctg ctcttcatgg gcgggatcgc
     5041 gacgctcggc gcgcttctgc ggccgcggcg ggtgacgcga ttctgggatc gcgcgctccc
     5101 cgccgacctg ttctcggccg agcgcgtgat ggcggcgggc gaggagacgg cgacgctcgc
     5161 gcacgtcgcg accgcgatcc gcggccagcg cgacgcgctc gagcgggcgc tccgggtgtc
     5221 cgtgccgctc gggctgagcg ggctcgcgat gaccgccctc gcgtacgcgc tcgagaaggc
     5281 cttctacgcg ccgtgagcct cgcgtgacgg cgcgcggatc ccgtcacggg gtctgacccc
     5341 gtaaacgcgg gcgccgtgcg tcttccgtga cgtttcgaga ggacgaaaac cgcccgattt
     5401 gcagggacgt cacgcttccg acaggaagtc gcgcgggtcc cgtcacgggg tctgaccccc
     5461 taagcgcgga cgagtcacgc ttccgtgaca ccttccggcg cggagaaacc acgctccgag
     5521 cggggacgtt acgaagccgg cacactttcg tgcgcggttt ggtgcggggt ctgaccccct
     5581 ttcgacgcgc gacctgccag gcgcgcgcgt gtatccgccc ggggctacga gccggtgacg
     5641 gccgacgggt tcggcgcgta ctcgagccag tgcgaccact gctcgcgctt gccccgcacc
     5701 gtgtcgaggt acgccgactg gatctcgcgc gtgatcggcc cggggtcgcc cacctcgcgg
     5761 tcgtccacgg cgcgcaccgg cgtcacctcg gccgcggtcc cgcacatgaa gatctcgtcc
     5821 gcgaggtaga ggtcggcccg cacgaggttc ttctcctcga ccgtgtagcc gagatcctgc
     5881 gcgatctgga tgaccgagtc gcgcgtgatg cccgggagga tcgacgacga gaggtccggc
     5941 gtcgcgatcc gcccgttctt cacgatgaag atgttctcgc cggagccgtc cgcgacgtag
     6001 ccggtctcgg tgaggaggat cgcctcctcg tagccggcct tcgacgcctc cagcaccgcg
     6061 agcatcgagt tcaggtagac gccggtcgcc ttggcgacgt gcgggatcac gttcgggccg
     6121 atccgctgcc agctcgagat cttcgcgcgc acgccgttct cgagcgcgtc atcgccgagg
     6181 tacgtccccc agggccagct catgatcacg acgtctacgg ggcagccggt cgggcgcacg
     6241 ccgagctcgc cgtagccgta gaacgcgagc gggcggaggt agcactcggg cagcccgttc
     6301 acgccgatca cctcgttgca ggccgccttg agctcctcga ccgagaaggg gagctccatc
     6361 tggatgagcc gcgcggagtt gtgcagccgc tgcaggtggt cggtgagccg gaagaccgcg
     6421 gagcccttct ccgtctcgta gcagcggatg ccctcgaaga cccccgagcc gtagtggagc
     6481 ccgtgcgcgc cgacgtggat cttggcgtcc gcccagtcga cgagctcgcc gttcatccag
     6541 atcttctcgg tctctctcat cgtgccctcc ctagtcttcg agccgggcgt ccacccggat
     6601 ctcgacgttg ccgcggagcg cgttcgagac gggacacgcc cgctcggcgt cctgcgcgtg
     6661 ccgcgagaag tctgacgcct cgagccccgg aacgcgaccc gtgacggtga gctcgctcgt
     6721 cgtgacgcgg agcccgtcgt catcgagctc ggccgagacg accgcggcga cctcgagccg
     6781 gtccggcggc gagccggcct ccgcgaggac gttcgagaac gccatcgcgt agcaggaggc
     6841 gtgcgccgcc gcgatgagct cctcagggct cgtcatcccg tccgcggcct ccgtgcgcgc
     6901 cgaccacgtg acggggagcg agaacgcgtt gctcgacgcg cccttcgcct cgccgcttcc
     6961 gcttgcgagc ggtccctccc agacgacctc ggctctgcgt tcgatggcca cgggttccct
     7021 cctcaggtcg tagtgtcgct gacagcgtcg acgacgcgcg cccgcgtgag ccgtgcgagg
     7081 tcggcggggg cgaggacggc catgtgccgc gggctgcccg cgccgaccca gacgacctcg
     7141 tgcgtgacga gcgtccggtc cagtagggat gcctccacgc ggccggccgg aaacggggcg
     7201 acgccgccga cgtcgaaccc cgtgacgtcg agcacctcct gcggggaggc gggcttggcc
     7261 ttcccgacgc cgagctcggc ggcgatcttc gcccggtcgg cgcgccggtc gccgggcacc
     7321 atgacgagcg cgtagcgctc cccgccgtcg agggcgaaga ggagcgactt gacgatctgc
     7381 tcgaggccgc agccggccgc ctccgccgcg gcctccgcgg tcggcgtgcc cgagcggaac
     7441 tcctccacgc gcccctcgat cccgttcgag cggagcacgg ccgcgacgcg ctccaccggc
     7501 tccggccagt cgcccgccat ctacggctcc gggaggacga tgaagcgcgc ctcgacgaac
     7561 cccggctcgg cgcgcagccg ttcgagcacg tcctccgagg ggacgtcgtc gagcgtaagc
     7621 accatgagcg cgcgccgctc gtgccggttc cgcgagacgg ccatgttcgc gatgttgatc
     7681 ccgctctcgc cgaggatcgt cccgatccgg ccgatcatcc ccggcgtgtc gtcgttctcg
     7741 gcgaagagca tctgcttgtc gagctcgacg tcgatctcgt agtcgaggac gcgggtgagc
     7801 tggcgccggt tctcctgccc gatcgtcgtc ccgtgggcgg cgtacgcgcg gccgtcggcg
     7861 tcgtaggcgg tcacggtcac gaggctcgtg aagtcgggcg aggacgcgct cgacgactcg
     7921 accacctcga gcccgcggct gcgcgcgatg agcggcgcgt tgacgaagtt caccgcctgc
     7981 tcgacgcgtc cctggaagac gccgttcagc gccgccgccg tgagcagccg cgtgtcgcgc
     8041 tcggcgaggc gcccgaggta gacgagctcg agccggttcg ggctgccgcc ggcgagcgcg
     8101 accgcgagcg cggccaggtc ggcgacgagc gggaggaacg ggccgagcac ggcgaagtcc
     8161 tccgccccga cggcgggcat gttcaccgcg ttcgtgacga cgctcccctc gagcgcggcc
     8221 gcgacctgct ccgcgacgat cacgccggcg cggtcctgcg cctcggcggt cgaggcgccg
     8281 aggtgcggcg tcaggatcac gttctcgaac tcggcgagcg gccccgtgta cggctcggcg
     8341 gcgaagacgt cgagcgccgc gccggcgacc cggccgtcgc gaagcgcctc gacgagcgcg
     8401 tcctcgacga cgagctcgcc gcgggcgacg ttcacgagcc ggagcccctc cctcgccttc
     8461 gtgagctcct cggcgccgat catcccgcgg ctctcctcgg tgagcgtcgc
//
LOCUS       DASTFC010000162         4639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1041234,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000162 DASTFC010000000
VERSION     DASTFC010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041234"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFO88_04885"
     CDS             complement(<1..629)
                     /locus_tag="VFO88_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET9544889.1"
                     /translation="MKGPRARRRERLLELDDVERRLKPFGRRYLGVREIALDALVGTD
                     GRAGSFTRDFRPLHAFSRDRLRSLKEAFADGGFPPIVAVKLGETYFVIDGHHRAALAR
                     RGGAEMIDADVTELIARVPLPAGADMLEVVLRELERIFLEDSGLAEARPGVRVSVSRP
                     AHYLELLENIQVHGYHMMRDRGRVLENAEIAANWHDAIYAPTLAAIDRLR"
     gene            complement(673..1560)
                     /locus_tag="VFO88_04890"
     CDS             complement(673..1560)
                     /locus_tag="VFO88_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9544890.1"
                     /translation="METTTNTRRLTFESGGETLVGNLSVPSEGEPAGVVIAVGPLTSV
                     KEQAAGTYARALAERGYAALAFDYRHFGESGGEPRQFESPEANVEDIRNAATALLDEW
                     LRDLPVYGLGICFGAGPMARAVAEDSRFRAFAGVAGVYTDGAKTRESMGDAYGARIER
                     GRAAERRWRETGEAETIPAVAPDGGDVAMPLREAYEFYGTPRGQVPNYVNGYAVQSLA
                     YSLPFDVRGAADTIEAPVLVVHSEQALAPGLAREFYAAVRSPKRDLWLRSEGQIDFYD
                     DPRLIGPAADAVAGFFQEA"
     gene            complement(1600..2469)
                     /locus_tag="VFO88_04895"
     CDS             complement(1600..2469)
                     /locus_tag="VFO88_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9544891.1"
                     /translation="MTARPLFGVGIPASARPVDDPVAFAVRAEELGFDFVSAPDHPAG
                     SAPTFETWTLLTWIAAATSRIGIAPRVLGVPYRAPAMVAKMAESFDRLSGGRLILGLG
                     AGSADDEHRAFGLGVRSPREKTTGLEEAITVVRGLWGGSGFTFRGELYSTDDAKIEPK
                     PARAIPIWLGTFAPRALAVTGRLADGWIPSLSYAGPDVVGGMRGRVLEAARRAGRDPA
                     EITCVYNVEVELRDLPGRETTRLVGPPAYVVERLHGFAELGFSAFNLIVGDDPERLDE
                     LAAEVVPLLRTAA"
     gene            complement(2466..3107)
                     /locus_tag="VFO88_04900"
     CDS             complement(2466..3107)
                     /locus_tag="VFO88_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544892.1"
                     /translation="MARAVVLFFGLLVFAAGIVALLESRLGLSPWDVLHDGLAARTGL
                     SFGAASVAVSLLVLVLAWFLGAPLGAGTVANAVLVGAFVEALTAIGAVERLADAPLAA
                     RFLLLALGVSLMAAGTALYLGAGLGAGPRDSLMVVGAERLGARVGLVRAALELSVLGA
                     GVGLGGTVGIGTIVFALGIGPAVEACFALLERSPLVRSPAGAEVGARPALEAA"
     gene            3234..4154
                     /locus_tag="VFO88_04905"
     CDS             3234..4154
                     /locus_tag="VFO88_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9544893.1"
                     /translation="MRWLGVELRHLLALRTVAEEGSLAAAARKLGYSQPAIGQQISSL
                     EQIVGSRLVERRTGGRSVELTEAGERVLGHGAAMLARAQAADADLQALRDGSAGTVRL
                     GTTQSVGARVVPELLRRLDAAGARITLELVEAATDEPLLAQLEAGELELTFAFAPLRP
                     GPFAALELLRDPYVLLVARTSPLAERRRPLGLRGLASTPLLVCRQGDAAEAFCRAHGI
                     AANVLHRIEDNELLVSLAAAGVGAALLPQLAVDPQRPDVVSVELAVELPSRSALLVRH
                     GDREPTEAVRVVTETIQRIGDELGDPPAAA"
     gene            4308..>4639
                     /locus_tag="VFO88_04910"
     CDS             4308..>4639
                     /locus_tag="VFO88_04910"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9544894.1"
                     /translation="MQITRNELETNRGPDDWFTGAVYIDTVATPTEGSRIAAACVHFT
                     PGARTAWHTHPHGQTIWVTEGVGLCQREGGPIEVIRPGDRVFFEPAENHWHGAAPTRF
                     MTHVALQQA"
BASE COUNT          659 a         1724 c         1684 g          572 t
ORIGIN      
        1 cgcaggcggt cgatcgcggc gagcgtcggc gcgtagatcg cgtcgtgcca gttcgccgcg
       61 atctccgcgt tctcgagcac gcgtccgcgg tcccgcatca tgtggtagcc gtgcacctga
      121 atgttctcca gcagctcgag atagtgcgcg ggccggctca ccgagacgcg cacgccgggg
      181 cgtgcctcgg caagcccgct gtcctcgagg aagattcgct cgagctcccg aaggacgacc
      241 tcgagcatgt ccgccccggc cgggaggggg acgcgcgcga tgagctcggt cacgtcggcg
      301 tcgatcatct ctgcgcctcc gcgccgcgcg agggcggcgc ggtggtgacc gtcgatcacg
      361 aagtacgtct caccgagctt gaccgcaacg atcggcggga agccgccgtc ggcgaacgcc
      421 tccttgagcg agcggagccg gtcgcggctg aaggcgtgaa gcgggcggaa gtcgcgggtg
      481 aacgagccgg cgcggccgtc cgtgccgacg agcgcgtcga gcgcgatctc gcgcacgccc
      541 aggtagcggc gcccgaacgg cttcagccgc cgctcgacgt cgtcgagctc gagcagtcgc
      601 tcgcggcggc gggccctcgg tcccttcatc gcgagaatgg tacaaccggc ccgttccctg
      661 ctcccgggcg gatcaggcct cctggaagaa gccggcgacc gcgtccgcgg cgggcccgat
      721 gagcctcggg tcgtcgtaga agtcgatctg cccttccgac cggagccaga ggtcgcgctt
      781 gggcgagcgc acggcggcgt agaactcgcg tgcgagacca ggcgcgagcg cctgctccga
      841 gtggacgacg agcaccggcg cctcgatcgt gtccgccgcg ccgcggacgt cgaacggcag
      901 cgagtaggca agcgactgca ccgcgtagcc gttgacgtag ttcggcacct gcccgcgcgg
      961 cgtgccgtag aactcgtacg cctcccgcag gggcatggcg acgtcgcccc cgtcgggagc
     1021 gaccgcgggg atcgtctcgg cctcgccggt ctcgcgccac ctgcgctcgg cggcccgccc
     1081 gcgctcgatc cgggcgccgt acgcgtcgcc catcgactct ctcgtcttcg cgccgtccgt
     1141 gtagacgccg gcgacgcccg cgaacgcgcg gaagcgcgag tcctccgcca ccgctcgcgc
     1201 catcgggccg gcgccgaagc agatgccgag cccgtagacg ggcaggtcgc gcagccactc
     1261 gtcgagcagc gccgtggccg cgttccggat gtcctcgacg ttcgcctccg ggctctcgaa
     1321 ctgccggggc tcgccgccgc tctcgccgaa atgccggtag tcgaacgcga gcgcggcgta
     1381 gccccgctcg gccagcgcac gcgcgtaggt gccggccgcc tgctccttca ccgacgtgag
     1441 cgggccgacc gcgatcacga ccccggcggg ctcgccctcg ctcggcacgg agaggttgcc
     1501 gacgagcgtc tcgcccccgc tctcgaatgt caggcgcctc gtgttcgtcg tcgtttccac
     1561 gctcttcctc cctttcgtcc ggttgccgtc cgtcgggctt cacgccgccg ttcgcaggag
     1621 cggcacgacc tcggccgcga gctcgtcgag cctctcgggg tcgtcgccga cgatcaggtt
     1681 gaaggccgag aacccgagct cagcgaagcc gtggagccgc tccaccacgt acgccggcgg
     1741 gccgacgagg cgggtcgtct cgcggccggg caggtcgcgg agctcgacct cgacgttgta
     1801 gacgcacgtg atctcggccg ggtctcgtcc ggcgcggcgg gccgcctcga gcacacggcc
     1861 gcgcatcccg ccgaccacgt cgggcccggc gtacgagagc gaagggatcc agccgtccgc
     1921 gagccttccg gtgacggcga gcgcgcgcgg cgcgaacgtg ccgagccaga tcgggatcgc
     1981 gcgggcgggc ttcggctcga tcttcgcgtc gtccgtgctg tagagctcgc cgcggaaggt
     2041 gaacccggag ccgccccaga ggccgcgcac gacggtgatc gcctcctcga ggccggtcgt
     2101 cttctcacgc ggcgagcgga cgccgagccc gaaggcgcgg tgctcgtcgt cggccgaccc
     2161 ggcgccgagg ccgaggatca gccggccgcc ggagaggcgg tcgaaactct cggccatctt
     2221 cgcgaccatc gccggggccc ggtagggaac gccgaggacc cgcggagcga tgccgatgcg
     2281 cgacgtggcg gcggcgatcc acgtgagcaa cgtccacgtc tcgaacgtcg gcgccgagcc
     2341 cgcgggatgg tccggggcgg agacgaagtc gaagccgagc tcctccgccc gcacggcgaa
     2401 cgcgacggga tcgtccaccg gccgcgcgga ggcgggaatc cccacgccga agagcgggcg
     2461 tgccgtcatg ccgcctccaa cgcgggacgc gcaccgacct cggcgcccgc gggagaacgc
     2521 acgagcgggg aacgctcgag gagcgcgaag caggcctcga ccgccggtcc gatcccgagc
     2581 gcgaagacga tcgtcccgat tccgaccgtt ccgccgagcc cgacacccgc gccgagcacg
     2641 gaaagctcga gcgccgcgcg gacgaggccg acccgggcgc cgaggcgctc ggcgccgacg
     2701 accatgagcg agtcgcgcgg ccccgccccg agcccggcgc cgaggtagag cgccgtgccc
     2761 gccgccatga gcgagacccc gagcgcgagc aggagaaacc gggcggcgag cggcgcgtcg
     2821 gcgagccgtt cgacggcgcc gatcgccgtc agcgcctcga cgaacgctcc gacgaggacg
     2881 gcgttcgcga cggtgccggc gccgagcggc gccccgagga accaggcgag caccaggacg
     2941 agcaggctca cggcgacgct cgccgcgccg aacgagagcc cggttcgcgc cgcgagcccg
     3001 tcgtggagca cgtcccacgg cgagagaccg agcctcgact cgagcagcgc gacgatgccc
     3061 gccgcgaata cgagcagccc gaagaagagg accaccgccc gcgccaacag cccgccgcga
     3121 gcccggggaa ggacacgccg gggtcggatc cagatcgcca tcacggtcta catctagcga
     3181 accgggcacc cctcactaca ctgatctttt cgatggaaga tcgaagcaac ggtttgaggt
     3241 ggctgggcgt cgagctgagg cacctgctcg ccctccggac cgtcgccgag gaaggctcgc
     3301 tcgcggcggc cgcacgcaag ctcggctaca gccagcctgc gatcggccag cagatctcgt
     3361 cgctcgagca gatcgtcggg tcgcggctcg tcgagcggcg caccggcggg cgctccgtcg
     3421 agctcaccga agcgggcgag cgggtgctcg gccacggcgc cgccatgctc gcgcgggcgc
     3481 aggccgccga cgccgatctg caggcgctgc gggacggttc cgcggggacg gtgcggctcg
     3541 ggacgaccca gagcgtcggg gcgcgcgtcg tccccgagct gctgcgccgc ctcgacgcgg
     3601 ccggcgcccg catcacgctc gagctcgtcg aggcggcgac ggacgagccg ctgctcgcgc
     3661 agctcgaagc cggggagctc gagctcacgt ttgccttcgc cccgctccgg ccgggaccgt
     3721 tcgccgcgct cgagctcctg cgcgatccgt acgtcctgct cgtcgcgagg acgtccccgc
     3781 tcgccgagcg ccgccggccg ctcggccttc gcgggctcgc gagcacgccg ctgctcgtct
     3841 gccgccaggg cgacgccgcg gaggccttct gtcgtgcgca cggcatcgcc gcgaacgtgc
     3901 tccaccggat cgaggacaac gagctgctcg tctcgctcgc cgccgcgggc gtgggcgccg
     3961 cgcttctgcc gcagctcgcg gtcgatccgc agcgccccga cgtcgtctcg gtcgagctcg
     4021 cggtcgagct tccgtcccgg agcgcgctcc tcgtgcgcca cggggaccgg gagccgaccg
     4081 aggccgtgcg cgtcgtgacc gagacgatcc agaggatcgg cgacgagctc ggcgatccgc
     4141 ccgcggccgc gtagccggct ccgtgaacgc gccggcccgg acggcggcgg ccgttcgacg
     4201 actcggcctc ggcctggccg gtgagcagga cctcccgagc gtgagctcgc cgacgaccgg
     4261 gtagcccctt cgcgagccgc ggccgcaagc aagcgaagga gagagccgtg cagatcacga
     4321 ggaacgagct cgaaacgaac cgcggcccgg acgactggtt caccggcgcg gtgtacatcg
     4381 acacggtcgc gacgccgacc gagggctcgc ggatcgccgc cgcgtgcgtc cacttcacgc
     4441 ccggagcgcg caccgcgtgg cacacgcatc cgcatggtca gacgatctgg gtgacggagg
     4501 gcgtcggcct ctgccagcgg gagggcggcc cgatcgaggt gatccggccc ggtgaccgcg
     4561 tgttcttcga gccggccgag aatcactggc acggcgcagc gccgactcgc ttcatgacgc
     4621 atgtcgcgtt gcagcaggc
//
LOCUS       DASTFC010000163         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1321399,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000163 DASTFC010000000
VERSION     DASTFC010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321399"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFO88_04915"
     CDS             <1..504
                     /locus_tag="VFO88_04915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9544895.1"
                     /translation="ERGLVLVNDLGTRGRRLGADVYRRVREEIPLDLVGMGSEDEPGG
                     LGEVPHDELPAFAARYRFLFNPIRYTSLGLAVLEAMAVGLPVVAVGATEHAVAIRDGV
                     SGYTETSVERLIARMRELLADHGEAARLGAAARRTARDRYSVERFAADWDGAFRAVVS
                     RAAAPVA"
     gene            complement(501..1985)
                     /gene="rfaE2"
                     /locus_tag="VFO88_04920"
     CDS             complement(501..1985)
                     /gene="rfaE2"
                     /locus_tag="VFO88_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET9544896.1"
                     /translation="MAAELPSLLDRFRGLRVVVVGEAMLDAYLEGGAARLSAEAPVPV
                     VAVEERRLLPGGAANTAANVRALGADVTLLSAIGDDGEGRLLREALGAHGVASASLLT
                     EEGRATQAKTRVVAGGHMLLRFDGGSTEPVGRDAEDALVDRLVEAAAAADAIVVSDYG
                     YGILTPRVVETLARLQREEERVLVVDSKDLRAFREAGVTAVKPNYRQALGLLGASDRS
                     GPRPELIAAEGERILAATGARIAAVTLDSEGALVLERGRDPYRTYARRHADSRAMGAG
                     DTFAATFALALAAGADTPAAAELASAAAGVVVAKDGTACCRADELLAAITGEGKVVAD
                     RERLAAVVARHREEGRRIVFTNGCFDLLHRGHVTYLSRAKTLGDVLVVGLNSDESVAR
                     LKGPDRPLNSLADRAHVLAALSCVDLVAPFPEETPERLLEAVRPDVFVKGGDYTRAML
                     PEAPLVERLGGRVELLPYVEDRSTTGIIERIRDGAPGHAERASL"
     gene            2037..2681
                     /locus_tag="VFO88_04925"
     CDS             2037..2681
                     /locus_tag="VFO88_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544897.1"
                     /translation="MATIPTDPAETLAALEASDRFLIQQVFRPVANEYRISVPAPGST
                     DEGAPLLFVKQKRMKIKEDIRFRVAADEERHLFMIKARTVFEFRGRHDVFDEQGAKIG
                     ALEKAFGKSLFRSHWIVRDELGEQVFEAHEASWPIALLRRFADLLPFDLLGLLTWLPF
                     NFVLVREGQKVATYTRVLGKLRDRYVLELGPELAGVDKRLVLAFAVALDALQDR"
     gene            complement(2678..>2859)
                     /locus_tag="VFO88_04930"
     CDS             complement(2678..>2859)
                     /locus_tag="VFO88_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544898.1"
                     /translation="LARGPVGRFPPEPARFVGGHVVRAAVARKEQADDEGRRADRVTR
                     ALAGLAPAGLVPVDD"
BASE COUNT          356 a         1074 c         1073 g          356 t
ORIGIN      
        1 gagcgcgggc tcgtcctcgt gaacgacctc ggcacccggg gccggcggct cggcgccgac
       61 gtctaccggc gcgtccgcga ggagatcccg ctcgacctcg tgggcatggg ctccgaggac
      121 gagccaggcg ggctcggcga ggtcccgcac gacgagctgc ccgcgttcgc cgcgcgctac
      181 cggttcctct tcaacccgat ccggtacacg agcctcggcc tcgcggtgct cgaggcgatg
      241 gcggtcgggc tccccgtcgt cgcggtcggc gccacggagc acgccgtcgc gatccgcgac
      301 ggtgtgagcg gctacacgga gacgagcgtc gagcgcctga tcgcccgcat gcgcgagctg
      361 ctcgccgacc acggcgaggc cgcccggctc ggcgccgccg cccggcgcac cgcgcgcgac
      421 cgctactcgg tcgagcggtt cgcggccgac tgggacggcg cgttccgcgc ggtcgtctcg
      481 cgcgccgccg cgccggtcgc ctagagcgag gcccgctcgg cgtggccggg ggcgccgtcg
      541 cggatccgct cgatgatccc cgtcgtcgag cggtcctcga cgtacgggag gagctcgacg
      601 cgaccgccga gccgctcgac gagcggcgcc tcgggcagca tcgcgcgcgt gtagtcgccg
      661 cccttcacga agacgtccgg ccggacggcc tcgagcagcc gctcgggcgt ctcctccggg
      721 aacggcgcga cgaggtcgac gcacgagagc gcggcgagca cgtgcgcgcg gtcggcaagc
      781 gagttcagcg gccggtcggg gcccttgagg cgggcgacgc tctcgtcgga gttcaggccg
      841 acgacgagca cgtcgccgag cgtcttcgcg cgcgagaggt acgtgacgtg gccgcggtgg
      901 agcaggtcga agcagccgtt cgtgaacacg atccgccggc cttcctcgcg gtggcgggcg
      961 acgaccgcgg cgaggcgttc ccggtcggcg acgaccttcc cctcgccggt gatcgcggcg
     1021 aggagctcgt cggcccggca gcacgcggtg ccgtccttcg ccacgacgac gccggccgcg
     1081 gcggacgcga gctcggcggc ggcgggcgtg tccgcgccgg cggcgagggc gagggcgaag
     1141 gtggccgcga acgtgtcgcc cgcgcccatc gcgcgcgagt cggcgtgccg ccgcgcgtac
     1201 gtccggtacg gatcacggcc gcgctcgagc acgagcgcgc cctcgctgtc gagcgtgacc
     1261 gcggcgatcc gcgcgccggt cgccgcgagg atccgctcgc cctcggcggc gatcagctcc
     1321 ggccgtggcc cgctgcggtc gctcgcgccg agcaggccga gcgcctggcg gtagttgggc
     1381 ttcacggccg tcacgccggc ctcgcggaac gcgcggagat ccttggagtc gacgacgagc
     1441 acgcgctcct cctcccgctg caggcgcgcg agcgtctcga cgacgcgggg cgtcaggatc
     1501 ccgtagccgt agtcggagac gacgatcgcg tcggcggccg cggcggcctc gaccagacga
     1561 tccacgagcg catcctcggc gtcgcgcccg accggctcgg tcgagccgcc gtcgaagcgc
     1621 aggagcatgt gcccgccggc gacgacgcgg gtcttcgcct gggtcgcgcg cccctcctcc
     1681 gtgagcaggg acgcgctcgc gaccccgtgg gcgccgagcg cctcgcggag gagccgcccc
     1741 tcgccgtcgt cgccgatcgc agagaggagc gtcacgtccg cgccgagcgc gcggacgttc
     1801 gcggcggtgt tcgcagcccc gccgggaaga agccgccgct cctcgaccgc gacgacgggc
     1861 accggcgcct cggcggagag ccgcgccgcg ccgccctcga ggtacgcgtc gagcatcgcc
     1921 tcgccgacga cgacgacgcg caacccgcgg aagcggtcga ggagcgaggg gagttcggcg
     1981 gccatacgcc gttctgcgcc ggacggtcga tccgaaacgc gctaccctcg acggccgtgg
     2041 ccacgatccc gaccgatccc gccgagacgc tcgccgcgct cgaggcctcc gaccgcttcc
     2101 tgatccagca ggtcttccgc ccggtcgcga acgagtaccg gatctccgtg cccgcgcccg
     2161 gctcgacgga cgagggcgcg ccgctcctct tcgtcaagca gaagcggatg aagatcaagg
     2221 aggacatccg cttccgcgtc gcggccgacg aggagcggca cctcttcatg atcaaggcgc
     2281 ggacggtctt cgagttccgt ggccgccacg acgtcttcga cgagcagggc gcgaagatcg
     2341 gcgcgctcga gaaggcgttc ggcaagtcgc tcttccgcag ccactggatc gtgcgcgacg
     2401 agctcggcga gcaggtcttc gaggcgcacg aggcgagctg gccgatcgcg ctcctgcgcc
     2461 gcttcgccga cctcctgccg ttcgacctgc tcgggctgct cacgtggctg cccttcaact
     2521 tcgtgctcgt gcgcgaggga cagaaggtcg ccacgtacac gcgtgtcctc ggcaagctcc
     2581 gcgaccgcta cgtcctcgag ctcggcccgg agctcgccgg cgtggacaag cggctcgtcc
     2641 tcgcgttcgc ggtcgcgctg gacgccctgc aggaccgcta gtcgtccacg gggacgagcc
     2701 cggcgggcgc gaggccggcg agcgctcgcg tgacgcggtc ggcgcggcgg ccctcgtcgt
     2761 cggcctgctc cttgcgcgcg accgccgccc gcacgacgtg gccgccgacg aagcgggccg
     2821 gctcgggcgg gaagcggccg acggggccgc gggcgaggc
//
LOCUS       DASTFC010000164         5960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_398513,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000164 DASTFC010000000
VERSION     DASTFC010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398513"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..317
                     /gene="rpsR"
                     /locus_tag="VFO88_04935"
     CDS             48..317
                     /gene="rpsR"
                     /locus_tag="VFO88_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9544899.1"
                     /translation="MAPKGKQQGGRSRRPQQGGGGGRRKSCYFCKDKIDQVDYKNYNQ
                     LRRYVSEKGKIRSRRITGACRRHQEQVAVAVKRAREMALLPYVSG"
     gene            322..1149
                     /gene="rplI"
                     /locus_tag="VFO88_04940"
     CDS             322..1149
                     /gene="rplI"
                     /locus_tag="VFO88_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9544900.1"
                     /translation="MDVILLKDVEQVGLRGEVVNVARGYMRNYLEPRGLAQPATPALL
                     GELRKREEQRARHEARSVEQAQEIADTLTKTVLRFEVKAGTRGRLFGSVTPTDIVDEL
                     WRARRIRVDRRKIDLPETIKRVGRYEVPIAIFEDVRVEVKTLVVPEGGELPSEDEIAA
                     WEAEERAEAEAAAGVTDEQAEVEAAVEAAIAAEEAELAAEAELAAEAGEGEAAEPGEG
                     EAAEAEAPEAPSAEEQVEAAWDAHESGDVAGQAEAEASVAAEADEAEQAEPADEPRP"
     gene            1395..2741
                     /gene="dnaB"
                     /locus_tag="VFO88_04945"
     CDS             1395..2741
                     /gene="dnaB"
                     /locus_tag="VFO88_04945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9544901.1"
                     /translation="MSQAVQTAPVPPQNLDAEESVLGAMMLSPGAIGAVSEVLDGDGR
                     DFYRESHAKIYKAALALYGKGEPVDAITLVDELDERGDLEAVGGSGRVHELATLVPAS
                     ANARHYAQIVHETAILRGLIQAGGEIARLGWERPGDAAALVDQAEQIVFELGQERASS
                     EFSAIEDLLKESFERITHLYELGADVTGVPSGFRDLDRITSGFQEGNLVIVAARPGMG
                     KSALGLGFAANVAVRTQQPVALFTLEMSKAEVTQRLMCAEAKVELHRLRTGRLAADDW
                     PRLTAACDRLAKAPLYVDDTGSITMMEIRSKARRLKSKHPDLGLIVVDYLQLMTSGTN
                     PENRVQEVSQISRSLKVLARDLEVPIIAMSQLSRAVEQRHDKRPILSDLRESGSIEQD
                     ADIVTFIYRDDYYNPEDSPDPGVAEVIVAKHRNGPTDKVKLSFISQYAKFADLAAA"
     gene            complement(2798..3079)
                     /locus_tag="VFO88_04950"
     CDS             complement(2798..3079)
                     /locus_tag="VFO88_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544902.1"
                     /translation="MNRLGIVTLACVLGLALVPAAPGAPPENPDCWDAASADLARSAP
                     GALGDHAANPPPIDLTPDRPGRAGIGNVARALGGDHPSDAAALLGFACP"
     gene            3187..4485
                     /locus_tag="VFO88_04955"
     CDS             3187..4485
                     /locus_tag="VFO88_04955"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9544903.1"
                     /translation="MPATVIVGAQWGDEGKGKIVDLLAEHSDVVCRYQGGPNAGHTIV
                     RDGETFKLHHIPSGILYPGRICVVGAGCVIDPGQLVREIDGLEERGIPTGDLRISGNA
                     HLIMPWHVAIDSASERRLGKLQIGTTRRGIGPAYADKAARIGIRVQDVLDPKILRQKF
                     ATALAEKNVMLERIHGVPALEVEDLADRMEEYAVRLRPYVADTSLLVDRALRDGQRVL
                     LEGAQGTLLDLDHGTYPFVTSSNPTAGAAATGTGIGPTRIDRVLGVAKAYVTRVGEGP
                     FPTEIEGPDQARMRDLGAEFGTTTGRERRCGWLDLVALRFAVRVNGLTSLAITKLDVL
                     SAFAELPVCVRYRLPDGSETEDFPAHQSDFHHARPVYETLAGWDEPLDGVASLDGLPG
                     AARRYVDFVAEALDVEVCMVGTGPDRERVLTSRALASVAS"
     gene            4525..5406
                     /locus_tag="VFO88_04960"
     CDS             4525..5406
                     /locus_tag="VFO88_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544904.1"
                     /translation="MRIVLLLTALSLLAAGCGAAEVGEVAEPEQAAAPPEDAEALYEA
                     TGTVLEAPEGGPMLCLGPMLMSLPPQCGDIPIRGWSWDAVEGEQRHGGVRWGEFHVVG
                     RYDGETFALVEAGPPRPAQAEPAEDLFVPPCPEPEGGWLAEGAAASQSEVDEAEGYAR
                     RQAEYVRSWVFHIGDPAALEEGAYEEDLPVVYVAVFTSEAARHEAELRTRWAGPLCVV
                     ERDVPSAREGARIRADAEASLEELGLQMLSSWEGELGLAAEIEVVADPGGRGQAALDE
                     RFGPGVVRLVPKLAPVG"
     gene            complement(5408..>5960)
                     /locus_tag="VFO88_04965"
     CDS             complement(5408..>5960)
                     /locus_tag="VFO88_04965"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET9544905.1"
                     /translation="LHGAVADLAEPVTGVLLVGGASRRFGSPKALAELDGEPFAVRIH
                     RTLAEAFPHVVAVGKAADRLDLPFPVEDDGSELRAALVGLVAGLRLSPTELAVVVPTD
                     VPFVTADYLRVLAAAAREATAAHPESGPLPGAYRRAALPVLERRLAAGELALRDAAAE
                     LEARVVPDDQELLRNVNTPADLP"
BASE COUNT          855 a         2132 c         2252 g          721 t
ORIGIN      
        1 cggcgccgac gacgacatcc cgttctagag aggagcacga gacccgcatg gcaccgaagg
       61 gaaagcagca gggcggccgc tcgcggcgtc cccagcaggg gggcggcggc ggcaggcgca
      121 agagctgcta cttctgcaag gacaagatcg accaggtcga ctacaagaac tacaaccagc
      181 tccggcgcta cgtctcggag aagggcaaga tccgctcgcg gcggatcacc ggcgcctgcc
      241 ggcgccacca ggagcaggtt gcggtcgcag tgaagcgggc gcgcgagatg gccctccttc
      301 cctacgtctc cgggtagcgc gatggacgtc atcctcctca aggacgtgga gcaggtcggg
      361 ctgcgcggcg aggtcgtgaa cgtcgcccgt ggctacatgc gcaactatct cgagccgcgc
      421 gggctcgccc agccggcgac gccggcgctc ctcggcgagc tgcgcaagcg ggaggagcag
      481 cgcgcccgcc acgaggcgcg cagcgtcgag caggcccagg agatcgcgga cacgctcacg
      541 aagacggtgc tccgcttcga ggtgaaggcc gggacgcgcg ggcggctctt cggctccgtg
      601 acgccgacgg acatcgtcga cgagctctgg cgggcgcggc gcatccgggt cgaccggcgc
      661 aagatcgatt tgcccgagac gatcaagcgg gtcggccgct acgaggtgcc gatcgcgatc
      721 ttcgaggacg tgcgcgtcga ggtgaagacg ctcgtcgtgc ccgagggcgg ggagctcccg
      781 agcgaggacg agatcgccgc gtgggaggcc gaggagcggg cggaggccga ggcggccgcc
      841 ggcgtgaccg acgagcaggc cgaggtggag gccgcggtcg aggccgcgat cgccgccgag
      901 gaggccgagc tcgccgcgga ggccgagctc gccgcggagg ccggcgaggg cgaggcggcc
      961 gagccgggcg agggcgaggc ggcggaggcc gaggcgcccg aggcaccgag cgccgaggag
     1021 caggtcgagg ccgcgtggga tgcccacgag tccggcgacg tcgccggcca ggccgaagca
     1081 gaggcgagcg tcgccgcgga ggcggacgag gccgagcagg ccgagcccgc cgacgagcct
     1141 cggccctagc ccgcggccga tccacaccgt cgtccccagg ccttcacggc ctgtgggcgc
     1201 gtgcgacgaa gttcccgctc ggagcgccgg tcctctccac aggcccgtat ccggttcggc
     1261 ggataccgtc cggcctccct gcgaacatca gttccgaaat ggctgcaaaa cgaccacgtt
     1321 cgcctgtgga cggacgcttc agtggacgcg cgtgacacgg cggcgggagt cctgtctaat
     1381 acggcgctcc ccgcatgagc caggccgtcc agaccgcccc cgtccccccg cagaatctcg
     1441 acgccgagga atccgtcctc ggcgcgatga tgctctcgcc cggcgcgatc ggcgccgtca
     1501 gcgaggtgct cgacggcgac gggcgcgact tctaccgcga gagccacgcg aagatctaca
     1561 aggccgcgct cgccctctac gggaagggcg agcccgtcga cgccatcacc ctcgtcgacg
     1621 agctcgacga gcgcggcgac ctcgaggcgg tcggcggcag cgggcgagtc cacgagctcg
     1681 cgacgctcgt ccccgcgagc gcgaacgcgc ggcactacgc gcagatcgtc cacgagacgg
     1741 cgatcctccg cggcctcatc caggcgggcg gcgagatcgc gcgcctgggg tgggagcggc
     1801 cgggcgacgc ggcggcgctc gtcgaccagg cggagcagat cgtcttcgag ctcggccagg
     1861 agcgcgcgtc gagcgagttc tccgcgatcg aggacctcct gaaggagagc ttcgagcgga
     1921 tcacgcacct ctacgagctc ggcgccgacg tgaccggcgt cccgtccggc ttccgcgacc
     1981 tcgaccggat cacgtccggc ttccaggagg gcaacctcgt gatcgtcgcc gcgcgccccg
     2041 ggatggggaa gtccgcgctc gggctcggct tcgccgcgaa cgtggccgtg cgcacccagc
     2101 agccggtcgc gctcttcacg ctcgagatgt cgaaggccga ggtcacgcag cggctcatgt
     2161 gcgccgaggc gaaggtcgag ctccaccggc tgcgcaccgg gcggctcgcg gccgacgact
     2221 ggccgcggct cacggccgcg tgcgaccgcc tggcgaaggc gccgctctac gtcgacgaca
     2281 cgggctcgat cacgatgatg gagatccgct cgaaggcgcg gcgtctcaag tcgaagcacc
     2341 ccgacctcgg cctgatcgtc gtcgactacc tccagctcat gacgtcgggg acgaacccgg
     2401 agaaccgcgt ccaggaggtc tcgcagatct cccgctcgct caaggtgctc gcgcgcgacc
     2461 tcgaggtgcc gatcatcgcc atgtcccagc tctcgcgcgc cgtcgagcag cgccacgaca
     2521 agcggccgat cctctccgac ctccgcgagt ccggctcgat cgagcaggac gccgacatcg
     2581 tcacgttcat ctaccgcgac gactactaca acccggagga ctcgccggac cccggcgtcg
     2641 ccgaggtgat cgtggcgaag caccgcaacg ggccgaccga caaggtcaag ctctcgttca
     2701 tctcgcagta cgcgaagttc gccgatctcg cggccgcgtg agcggcgccg cgggggcggg
     2761 aatcaccagc accctcgccc ccgccgacgc agcaggccta cgggcaggcg aagccgagca
     2821 gcgcggccgc gtcgctcgga tggtcgccgc cgagcgcgcg cgcgacgttc ccgatcccgg
     2881 cgcggccggg gcggtcgggc gtgaggtcga tcggcggggg attcgccgcg tggtctccga
     2941 gcgcccccgg tgccgagcgg gcgagatccg cgctcgctgc gtcccagcag tccggattct
     3001 ccggcggggc acccggtgcg gccgggacga gcgcaagacc gagcacgcag gccagcgtca
     3061 cgattccgag acggttcatc gagacctcct tgcgtcgacg cgtcgaatga tgccgtccgc
     3121 aggcgggcct cgccagggcg cgcgaccggc cggcctcctg tgagtcccgg gtctaagctc
     3181 cagcccatgc ccgcgaccgt gatcgtcggc gcccagtggg gcgatgaggg caagggcaag
     3241 atcgtcgacc tcctcgccga gcactcggac gtcgtctgcc gctaccaggg cggcccgaac
     3301 gccgggcaca cgatcgtccg cgacggggag acgttcaagc tccaccacat cccgtccggg
     3361 atcctctacc ccggccgcat ctgcgtcgtc ggagcggggt gcgtgatcga cccgggccag
     3421 ctcgtccgcg agatcgacgg gctcgaggag cgcgggatcc cgaccggcga cctgcggatc
     3481 tcgggcaacg cgcacctgat catgccgtgg cacgtcgcca tcgactcggc gagcgagcgg
     3541 cggctcggga agctgcagat cgggacgacg cggcgcggga tcgggccggc ctacgcggac
     3601 aaggcggcgc ggatcggcat ccgcgtccag gacgtgctcg acccgaagat cctgcgccag
     3661 aagttcgcga cggcgctcgc ggagaagaac gtcatgctcg agcggatcca cggcgtcccg
     3721 gcgctcgagg tcgaggacct cgccgaccgg atggaggagt acgccgtgcg gctgcgcccg
     3781 tacgtcgcgg acacgtcgct tctcgtcgac cgcgcgctcc gcgacgggca gcgcgtcctg
     3841 ctcgagggcg cgcagggcac gctgctcgac ctcgaccacg ggacgtatcc cttcgtcacc
     3901 tcgtcgaacc cgaccgccgg cgccgcggcg acggggacgg ggatcgggcc gacgcggatc
     3961 gaccgcgtgc tcggcgtggc gaaggcgtac gtgacgcgcg tcggcgaggg gccgttcccg
     4021 accgagatcg aggggcccga ccaggcgcgg atgcgcgacc tcggcgcgga gttcggcacg
     4081 acgaccgggc gcgagcggcg gtgcggatgg ctcgacctcg tcgccctccg gttcgcggtg
     4141 cgcgtgaacg ggctcacgtc gctcgcgatc acgaagctcg acgtcctgtc ggcgttcgcg
     4201 gagctgccgg tctgcgtccg ctaccggctc ccggacgggt cggagacgga ggacttcccc
     4261 gcgcaccaga gcgacttcca ccacgcgcgg ccggtgtacg agacgctcgc cgggtgggac
     4321 gagccgctcg acggggtcgc ctcgctcgac gggctccccg gggccgcgcg gcgctacgtc
     4381 gacttcgtcg ccgaggcgct cgacgtcgag gtctgcatgg tcggcaccgg gccggaccgg
     4441 gagcgggtgc tgacctcgcg ggcgctcgcg agcgtcgcgt cgtagcggcg ggaacctttg
     4501 gcgcgcccgc agcgtcggaa gggcgtgcgg atcgtgcttc tcctgacggc gctctcgctg
     4561 ctcgccgcag gctgcggcgc cgccgaggtc ggcgaggtcg ccgagccgga gcaggcggcc
     4621 gcgccgcccg aggacgccga ggcgctgtac gaggcgaccg ggacggtgct cgaggcgccc
     4681 gaaggcggcc cgatgctctg cctcgggccg atgctcatgt ccctcccgcc gcagtgcgga
     4741 gacattccga tccgcggctg gagctgggac gcggtcgagg gcgagcagcg ccacggcggc
     4801 gtgcgctggg gcgagttcca cgtcgtcggc cgctacgacg gcgagacctt cgcgctcgtc
     4861 gaggccggtc cgccccgccc ggcgcaggcg gagccggccg aggacctgtt cgtcccgccc
     4921 tgcccggagc ccgaaggcgg ctggctcgcc gagggcgcgg ccgcctcgca gtcggaggtc
     4981 gacgaggccg aggggtacgc gcggcggcag gcggagtacg tccgctcgtg ggtcttccac
     5041 atcggcgacc ccgccgcgct cgaggaaggc gcgtacgagg aggacctgcc cgtcgtctac
     5101 gtcgcggtct tcacgagcga ggccgcccgc cacgaggcgg agctccgcac gcgctgggcg
     5161 ggcccgctct gcgtcgtcga gcgcgacgtt ccgagcgcgc gcgagggcgc ccggatccgc
     5221 gccgacgccg aggcgtcgct cgaggagctc gggctgcaga tgctgtcctc gtgggagggc
     5281 gagctcgggc tcgccgccga gatcgaggtc gtcgccgacc cgggcggccg cgggcaggcg
     5341 gccctcgacg agcgcttcgg gcccggggtc gtccggctcg tgccgaagct cgctccggtc
     5401 ggctgactca ggggagatcg gcgggcgtgt tgacgttccg gaggagctcc tggtcgtccg
     5461 ggacgacgcg tgcctcgagc tcggcggcgg cgtcgcgcag ggcgagctcg ccggccgcga
     5521 ggcggcgctc gaggaccggg agcgcggcgc ggcggtacgc gcccgggagc gggccgctct
     5581 ccgggtgggc ggcggtcgcc tcgcgcgccg cggccgcgag cacgcggagg tagtccgccg
     5641 tcacgaacgg gacgtcggtc ggcacgacga ccgcgagctc ggtgggcgag agccgaagcc
     5701 cggcgacgag cccgacgagc gccgcgcgca gctcgctccc gtcgtcctcg accgggaacg
     5761 gcaggtcgag tcggtcggcc gccttcccga ccgcgacgac gtgcgggaac gcctcggcaa
     5821 gcgtccggtg gatgcgcacc gcgaacggct ccccgtcgag ctcggcgagc gccttcggcg
     5881 agccgaaccg cctgctcgcc ccgccgacga gcaggacgcc cgtcacgggc tcggcgagat
     5941 ccgccacggc tccgtgtaga
//
LOCUS       DASTFC010000165         5864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_134971,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000165 DASTFC010000000
VERSION     DASTFC010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134971"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFO88_04970"
     CDS             complement(<1..505)
                     /locus_tag="VFO88_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544906.1"
                     /translation="MNEEATHRALLRARARVLVLAAAVAGALLVAVPATATPDGADTS
                     TAPWIASDKPDYAPGERVTLNGGNWEPGESVRIEVEDDQGKTWQRDVTVVADAAGAIS
                     DSFDLPDWFVATYSVRATGSSGATAASGFTDGNVQVTTNAGGPTVTVSYARYTAANCS
                     GAPFATGT"
     gene            769..2028
                     /locus_tag="VFO88_04975"
     CDS             769..2028
                     /locus_tag="VFO88_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544907.1"
                     /translation="MATAAANEATALFAALHSGAGALCRLAEEGRLSEEGARALADEV
                     ASAFGLGGDVVRAELCSRTLGDARVPALPPRLAVETVVRLLAALTPVRGASLWASGGD
                     CLSAAGSPSAEARSLATAALAGRAGHGDAVTAPVRSWGRTAAALVLEAAPGAHPLCRD
                     LAAEAAGALALVLEREALLDRNRERERSLVEASERRLARLAFDIHDGPIQIVAALGLE
                     LRLLGDGSDARGRDRALRELHERLAALEEELRELTQTLEPTSIARRPLDASLAGQAEA
                     FGRQHGITVSVETAGDLEGLTPSQRIALVRVVHEALTNARDHGGATDVRVAVRGDRRA
                     VTARVTDNGCGFDVASALAAAAGKGRLGLVGMSERVRLLGGRLEVESRPGGPTTIEAY
                     LERWSPSAAAAAPAAPTFAARAVPAPA"
     gene            2105..3487
                     /locus_tag="VFO88_04980"
     CDS             2105..3487
                     /locus_tag="VFO88_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9544908.1"
                     /translation="MRRPHPFQAFVGGTAVVLAVLVFGGPLVHPDPALRATMELGVGL
                     AALLAALLAFRRYAERGGVDGLVLGASLLALAFASLTAGALPVFAPQETAASLAWMRV
                     PPLLLAAAGLAVAARLSGRSLAPRPAHVAGAAGAVVVLQVAAGAVALALAGRLPADLG
                     ERVAEGAYSVPEDVAGVALAVHGLAAGLLLLAAVGFARAAVGGDRLARWLGFGCALAA
                     VAHVHYALHPSLVSSALSVGDVFRLAFAACLLAATVQEVAEHQRTLLAAAVARERRRL
                     ARELHDGLAQELSFLVTQTRLLAARHGRLDGLDALEDTSRRALDESRLAIGALARPGD
                     EPLDVALEAAVADLAERMGTSVRLRLSHGVDVEPPTRDALLRIVREAMTNAVRHGGAT
                     AVSVELEAGSGLRLRVVDDGQGFAVDDAAGAPAGFGLVSMRERAEALGGQLTVASRPG
                     EGTRVELVLP"
     gene            3484..4152
                     /locus_tag="VFO88_04985"
     CDS             3484..4152
                     /locus_tag="VFO88_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544909.1"
                     /translation="MSGPITVVVADDHVPARAGVRAALETGRFAVVAEAHDAAGAVAA
                     ALAHEPDVCLLDIHMPGGGIAAVAQIAARLPGTAVVMLTVSEDDDDFFDALRAGARGY
                     LLKDVDPLRLPFALEGVLAGEAALPRMLTARLVDRFREREVRRVPIEGSRTATLSARE
                     WEVLELLRQGLRTRQVAERLFLTEATVRSHVASVVRKLKVPDRAAAVRLLEEPPNGRL
                     AARR"
     gene            complement(4157..4516)
                     /locus_tag="VFO88_04990"
     CDS             complement(4157..4516)
                     /locus_tag="VFO88_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544910.1"
                     /translation="MCGGGTGGIVAGGRKGGALSYAFFSVSFVVLAVAMTLWIDLRFR
                     RLSPSGLRAGGLRLLAAVVVAQVALPVVARVVAPLPNAVESWTIVGAGFAAMVFLMLA
                     VVWMLRLAQGLMGGMLR"
     gene            complement(4568..5110)
                     /locus_tag="VFO88_04995"
     CDS             complement(4568..5110)
                     /locus_tag="VFO88_04995"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET9544911.1"
                     /translation="MSEAGTADAGAVARRGEALTAAAAGCLVAAALAERGLGAEGLVT
                     AFFLGVLAVLAAIDLERRVVPDRIVLPAAAIVLAGHLAFAPERWLEWLLAPLVAFAVL
                     FIPLLVRPSGIGFGDVKLMLLLGAGLGFAVLDALLIGFALVVPVALFLLVRDGPKARK
                     RSVPLAPFLAGGAILAIFLG"
     gene            complement(5107..5469)
                     /locus_tag="VFO88_05000"
     CDS             complement(5107..5469)
                     /locus_tag="VFO88_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET9544912.1"
                     /translation="MAAARVILRDRSAVVCERCVVADRPHTRLRGLLGRRELPAGEGL
                     LLRPAGAVHTSFMRFPIDVVFLDRNLTVVGHVDGLRPWRAAGRRGARAVLELAAGERA
                     RRAIAPGDRLALVHGTAS"
     gene            complement(5475..>5864)
                     /locus_tag="VFO88_05005"
     CDS             complement(5475..>5864)
                     /locus_tag="VFO88_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544913.1"
                     /translation="LADPAIEIYTAGRADVAAGRTDVRILALLRYLRIAHGSVTVSSL
                     TSGHRIYARPGVVSAHVYGLAVDIVALGGRPILGNQAPGGVTERAVRDVLLLPAELRP
                     QQVISLLGLGGPSFPLADHGDHIHVGY"
BASE COUNT          681 a         2208 c         2277 g          698 t
ORIGIN      
        1 ccgtgcccgt ggcgaacggg gctccgctgc agttcgccgc ggtgtagcgc gcgtacgaga
       61 cggtcacggt cgggccgccc gcgttcgtcg tcacctgcac gttgccgtcc gtgaagcccg
      121 acgccgccgt cgcgccgctc gacccggtcg cgcggaccga gtacgtcgcg acgaaccagt
      181 ccggaaggtc gaaggagtcg gagatcgcgc cggcggcgtc ggcgacgacg gtgacgtcgc
      241 gctgccatgt cttgccctgg tcgtcctcga cctcgatccg gacggactcg cccggctccc
      301 agttgccgcc gttcagcgtc acgcgctcgc cgggcgcgta gtccggcttg tcgcttgcga
      361 tccacggcgc ggtgctcgtg tcggcgccgt caggcgtcgc cgtggccggc acggcgacga
      421 gcagggcgcc ggcgaccgca gccgccagga cgagcacacg ggcgcgcgcg cggagcaggg
      481 ctcgatgtgt cgcttcctcg ttcacggttg accctccccg gagccgcagg cccctctcgt
      541 ccgtcccgcc gccggctcgc cggccgcgga gacccctttt gtcgagcgtg cccttgcccc
      601 gtggaaccgt ccatgggccg tccggcccat cctgcgccga cttggctcaa cggccgcgtg
      661 gacgaggctc cgcgcgcgct gggccggtcg gcccattgcc gcgctccgcg ttcgcggcca
      721 cgctgatggt tcgaggagag aactgctcgt agaccggagg gggactctgt ggcaacggct
      781 gcggcgaacg aggcgacggc gctctttgcg gcgctccatt cgggcgccgg tgcgctctgc
      841 cgcctggcgg aggaaggacg cctgtcggag gagggcgccc gcgcgctcgc ggacgaggtg
      901 gcgtcggcct tcgggctcgg cggcgacgtc gtccgggccg agctctgctc gcgcacgctc
      961 ggcgacgccc gcgtgccggc gctgccgccg cgcctggcgg tcgagacggt cgtccgcctg
     1021 ctcgcggcgc tgacgcccgt gcggggcgcg tcgctctggg cgtccggcgg cgactgcctc
     1081 tccgcggccg ggagcccgtc ggccgaggct cgctcgctcg cgacggccgc cctcgccggt
     1141 cgcgccgggc acggcgacgc cgtgacggcg ccggtccgct cgtgggggcg gaccgcggcc
     1201 gcgctcgtcc tggaggccgc gccgggcgcg cacccgctct gccgggatct cgcggccgag
     1261 gcggccggcg cgctcgcgct cgtgctcgag cgcgaggcgc tgctcgaccg gaaccgcgag
     1321 cgggagcgaa gcctcgtcga ggcgagcgag cggcggctgg cacggctcgc cttcgacatc
     1381 cacgacgggc ccatccagat cgtcgcggcg ctcgggctcg agctgcgcct gctcggggac
     1441 ggctcggacg cgcggggccg cgaccgcgcg ctccgcgagc tgcacgagcg cctcgccgcg
     1501 ctcgaggagg agctccgcga gctgacccag acgctcgagc cgacgtcgat cgcccgccgg
     1561 ccgctcgacg cgtcgctcgc gggccaggcg gaggcgttcg gcaggcagca cgggatcacc
     1621 gtgtcggtcg agacggcggg cgacctcgag gggctgacgc cgtcgcagcg gatcgcgctc
     1681 gtgcgcgtcg tccacgaggc gctcacgaac gcgcgcgacc acggcggcgc cacggacgtg
     1741 cgcgtcgccg tgcgcggcga ccggcgcgcc gtgaccgcac gggtgacgga caacggctgc
     1801 ggcttcgacg tggcgtccgc gctcgcggcc gccgcgggca aggggcggct cgggctcgtc
     1861 gggatgagcg agcgggtgcg cctcctcgga gggcgcctcg aggtcgagag ccggccgggc
     1921 ggcccgacga cgatcgaggc gtacctcgag cgctggagcc cgtccgcggc cgccgccgcg
     1981 cctgccgccc cgacgttcgc ggcgagagcc gtgcccgccc cggcctagcg cctcatcccg
     2041 gcgcgagcgg ccccggcggg ggatggacaa ggcacgagac gactgttacg tacagagggt
     2101 gggcatgcgg cggccccatc ctttccaggc gttcgtcggc ggcacggcgg tcgtcctcgc
     2161 cgtgctcgtt ttcggcgggc ctctcgtcca tcccgacccc gcgctccggg ccacgatgga
     2221 gctcggcgtc gggctcgcgg ccctcctcgc cgcgctgctc gccttccggc gctacgccga
     2281 gcgcggcggg gtcgacgggc tcgtcctcgg cgcgtcgctt ctcgcgctcg cgttcgcgtc
     2341 gctcaccgcc ggtgcgcttc ccgtcttcgc cccgcaggag accgcggcct cgctcgcctg
     2401 gatgcgggtc ccgccgcttc tccttgcggc ggccggtctc gccgtcgcag cgcggctgtc
     2461 cggcaggagc cttgctccgc gcccggcgca cgtggccggg gcggcgggcg cagtcgtcgt
     2521 gctgcaggtt gccgcggggg cggttgccct cgcgctcgcc ggccgcctcc ccgcagacct
     2581 cggggagcgc gtggcagagg gcgcgtactc ggtgccggag gacgtcgcgg gcgtcgcgct
     2641 cgccgtccac ggcctcgccg cgggcctgct gctcctcgct gccgtcgggt tcgcgcgcgc
     2701 cgccgttggc ggcgaccgcc tcgcccgctg gctcggcttc ggctgcgcgc tcgccgccgt
     2761 cgcccacgtg cactacgcgc tccacccgtc gctcgtctcg agcgccctct cggtcggcga
     2821 cgtcttccgc ctcgcgttcg cggcctgcct gctggccgcc accgtgcagg aggtcgccga
     2881 gcatcagcgc acgctcctcg ccgccgcggt cgcgcgcgag cgccgccggc tcgcgcgcga
     2941 gctccacgac gggctggcgc aggagctctc gttcctcgtc acgcagacgc ggctcctcgc
     3001 cgcgcggcac ggacggctcg acgggctcga cgcgctcgag gacacgtcgc ggcgggcgct
     3061 cgacgagtcg cggctcgcga tcggcgcgct cgcgcggccc ggcgacgagc cgctcgacgt
     3121 cgcgctcgag gcggcggtcg cggacctcgc ggagcggatg gggacgagcg tccggctgcg
     3181 gctctcgcac ggcgtggacg tcgagccgcc gacgcgcgac gcgctgctca ggatcgtgcg
     3241 cgaggcgatg acgaacgcgg tccggcacgg cggcgcgacc gccgtgagcg tcgagctcga
     3301 ggcgggcagc gggcttcgcc tccgcgtggt cgacgacggg caggggttcg ccgtggacga
     3361 cgcggccggc gccccggccg gcttcggcct cgtgagcatg cgcgagcgcg ccgaggcgct
     3421 cggcgggcag ctgaccgtcg cgtcgcgccc gggagaggga acccgagtcg agctggtgct
     3481 gccgtgagcg ggcccatcac cgtcgtcgtc gccgacgacc acgtccccgc gcgcgcgggc
     3541 gtccgcgcgg cgctcgagac gggccggttc gccgtggtcg ccgaggcgca cgacgcggcc
     3601 ggcgccgtgg ccgcggccct cgcgcacgag ccggacgtct gcctgctcga catccatatg
     3661 ccgggcggcg ggatcgcggc cgtcgcgcag atcgcggcgc ggcttcccgg cacggcggtc
     3721 gtgatgctga cggtctccga ggacgacgac gacttcttcg acgcgctgcg ggccggcgcg
     3781 cgcggctacc tcttaaagga cgtcgatccc ctgcggctcc cgttcgcgct cgagggcgtc
     3841 ctggccggcg aggccgcgct tccgcgcatg ttgacggcgc ggctcgtcga ccggttccgg
     3901 gagcgggagg tgcgccgcgt gccgatcgag ggctcgcgca cggcgacgct gtcggcgcgc
     3961 gagtgggagg tgctcgagct cctacgtcag gggctgcgca cgcggcaggt ggccgagcgg
     4021 ctcttcctca ccgaggcgac cgtgcggagc catgtggcgt cggtcgtccg gaagctgaag
     4081 gtccccgacc gcgcggccgc ggtcaggctg ctcgaagagc cgccgaacgg gcgactcgcg
     4141 gcgcgacgct aggcccctag cggagcattc cgcccataag cccctgggcg aggcggagca
     4201 tccagacgac cgcgagcatg aggaagacca tcgcggcgaa gccggcgccg acgatcgtcc
     4261 acgactcgac cgcgttcgga agcggcgcga cgacgcgggc cacgaccggc aacgcgacct
     4321 gcgcgacgac gacggcggcg aggaggcgga gcccgccggc acgcaggccg gaggggctca
     4381 gcctgcggaa gcgcaggtcg atccagagcg tcatcgccac cgcgagaacg acgaagctga
     4441 cggagaagaa cgcgtagctc agggcgcctc ccttgcgacc gccggcgacg ataccacccg
     4501 taccgccgcc gcacacgctc gatcgaggcg ctacccggcc cggcctggcc ctacacgcgc
     4561 cgcgtggcta gccgagaaag atggcgagga tcgcgccgcc cgcgaggaac ggggcgagcg
     4621 gcaccgagcg cttccgggcc ttcgggccgt cccggacgag caggaagagc gccacgggga
     4681 cgacgagcgc gaacccgatg agcagcgcgt cgagcacggc gaagccgagg ccggcgccga
     4741 gcaggagcat gagcttcacg tcgccgaacc cgatgcccga cgggcggacg aggagcggga
     4801 tgaagagcac cgcgaacgcg acgagcggcg cgagcagcca ctcgagccac cgctcgggcg
     4861 cgaacgcgag gtgcccggcg aggacgatcg ccgcggcggg caggacgatc cggtccggca
     4921 cgacgcgccg ctcgaggtcg atcgcggcga gcacagcgag cacgccgagg aagaacgccg
     4981 tcacgagccc ctccgcgccg agcccgcgct cggccagggc ggctgcgacg aggcagccgg
     5041 cggcggccgc ggtcagcgcc tctccgcgac gcgcgaccgc tcccgcgtcc gcggtgccgg
     5101 cctcgctcac gaggcggtcc cgtggacgag ggcgaggcgg tcgccggggg cgatcgcccg
     5161 gcgggcgcgc tccccggcgg cgagctcgag caccgcgcgc gctccccgcc gcccggccgc
     5221 gcgccagggc cggagcccgt ccacgtggcc gacgaccgtc aggtttcggt cgaggaagac
     5281 gacgtcgatc gggaagcgca tgaacgatgt gtgcacggcg ccggcgggcc ggaggagaag
     5341 cccctcgccg gcgggcagct cgcgccggcc cagcaggccg cgcaggcgcg tgtgcgggcg
     5401 gtcggcgacg acgcaccgct cgcagacgac cgccgaccgg tcgcgcagga tcacacgcgc
     5461 cgccgccacc ggcgctagta ccccacgtgg atgtggtcgc cgtggtcggc gagcgggaac
     5521 gacggcccgc cgaggccgag gagcgagatc acctgctgcg gccggagctc ggccggcagg
     5581 agcaggacgt cacgcacggc ccgctcggtc acgccgccgg gggcctggtt cccgaggatc
     5641 gggcgcccgc cgagcgcgac gatgtcgacc gcgagcccgt agacgtgcgc cgagacgacg
     5701 ccgggccgcg cgtagatcct gtgcccgctc gtgaggctcg agacggtgac gcttccgtgc
     5761 gcgatccgga ggtagcggag cagcgcgagg atccggacgt cggtccgccc cgcggcgacg
     5821 tcggcgcgcc cggccgtgta gatctcgatc gcgggatccg cgag
//
LOCUS       DASTFC010000166         5162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_415228,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000166 DASTFC010000000
VERSION     DASTFC010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415228"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFO88_05010"
     CDS             complement(<1..740)
                     /locus_tag="VFO88_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET9544914.1"
                     /translation="MRRPELDDLREARGRLTGSARETPVFASETLKRLSGRDVYLKAE
                     NLQRTGSFKVRGAMSKIATLGPGEREAGVVTASAGNHGQAVAWAAREAGIRATIFVPD
                     DAPMAKVEAARGYGAEVVMGGSGYDEAYEASKERAEGGATFVHPFEDTEVIAGQGTLG
                     LELAEGLPEDLGAVAVPVGGGGLASGVAIALRALRPDVRLVGVQAAACAPLAGGEPGG
                     FTIADGIAVKKPGELTSAILADLLDDVVT"
     gene            complement(727..1704)
                     /gene="meaB"
                     /locus_tag="VFO88_05015"
     CDS             complement(727..1704)
                     /gene="meaB"
                     /locus_tag="VFO88_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9544915.1"
                     /translation="MSTRAAWTAESLLEGLRSGDRRALARAISLVENGDPLAGELVRE
                     TYPQTGRAEIVGMTGPPGVGKSSLVSALVRLVRGEGRTIGVISVDPSSPFTSGALLGD
                     RIRLSDHFLDRDVFIRSMGTRGHLGGLAEATLQAALLLDAFGKDVVFVETVGTGQSEV
                     EITGLADTIVLALMPGSGDSIQALKAGIMEIPDVIVVNKRDHPAAKTMMNEVRSILGL
                     GEKREWTPPIVLTEAVRGEGIEELWEAVGNHRRHLVESGRLEERRGEQLAGEVFAVAS
                     GRAKTHLERAVADDPELRRLLDEVKGRRLDPLTAVREILEEVFRLDETT"
     gene            complement(1701..2822)
                     /gene="hisC"
                     /locus_tag="VFO88_05020"
     CDS             complement(1701..2822)
                     /gene="hisC"
                     /locus_tag="VFO88_05020"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET9544916.1"
                     /translation="MAQGDLFRPAIADLVPYEPGKPVEEVQRELGLDRVVKLASNEGQ
                     FGPFPAAVEALQRSVNELNRYPDGGMYRLTTALAERHGVEPERVAIAAGADAVIMYLS
                     LAALDPGDEVVCGWPSFPSYVIDAIKMGATPVRVPLRDLTYDLEALLAAVTPRTKIVF
                     VANPNNPTGTMVGRAELDRYFDAVPEHVLTVLDEAYFEYVDEPDYPDGIEEYLKRGGR
                     RVLCLRTFSKIYGLAGLRVGYGVGPVDVVAAIRKVRNAFDVSQPAQDAALASLGEPEE
                     LARRRAVTAEGRARLEAVCSRLPVEICPRPVANFVYVDCGREVAPLFDALLREGVIVR
                     PLGPFGAPAAMRVTVGEPEENDLFGEALERCLTAAAAAR"
     gene            2891..3502
                     /locus_tag="VFO88_05025"
     CDS             2891..3502
                     /locus_tag="VFO88_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9544917.1"
                     /translation="MEEIGLFPLPLVLVPGEQAPLHIFEPRYKELVDECLDTAAPFGF
                     LLADDDGVREVGTLAEVVEVVERFDDGRLNIVVEGAARFRVARLTDGRSFQTAEVDTV
                     DDEGDEPTDDERARCLAGYDKVIAAAAAELEELDRDADDLSFRIAARIDLGPEIKQTL
                     LESRSERERVVRLAGLLDRAAEAVAREREVRERASGNGRVEAL"
     gene            3755..4567
                     /locus_tag="VFO88_05030"
     CDS             3755..4567
                     /locus_tag="VFO88_05030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9544918.1"
                     /translation="MPAAIQSRFVLAAAAVVFAAGFVAFALIPTDDVGIPHVFYIAIA
                     LVAAVTGPLWGAAAGALAAALYCIGMIVHPIVPSAEILTGGTLVRLATYACTGALIGW
                     FARDNRRLVAELRILADRDTVTGLPNTRAFEAAIQRRLDAQRPFALLIGDLRGVDDAS
                     LGEDVAANDALLRIGDVLGNAVGPGDDLARVGGRAFALLSSLGSAHEAARLAGRLEQT
                     LAGHGLPTRFGWAVFPHEGANALSLYRAADERLYARKLVSADAADVGLRSVS"
     gene            complement(4564..4932)
                     /locus_tag="VFO88_05035"
     CDS             complement(4564..4932)
                     /locus_tag="VFO88_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544919.1"
                     /translation="MTEPLEPGRGTLLTEEEERVEEEELWTGDEGTDHTGWYCVATDP
                     FPCPAAGCDFVASFMTAAHLILVWEERDDPNLLRHAQRAKEVGRNPRVVQYRPEFGPS
                     ASYYAWEASGRPVHGVRGRA"
     gene            complement(4929..>5162)
                     /locus_tag="VFO88_05040"
     CDS             complement(4929..>5162)
                     /locus_tag="VFO88_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544920.1"
                     /translation="LNDARDAYAAEEESGRDFSDGPCIADPLPDREDWVVVVVREPRD
                     EDRNAAERCSAYRDGTAEHFVELDEFGHVIRAQ"
BASE COUNT          654 a         1960 c         1846 g          702 t
ORIGIN      
        1 gtgacgacgt cgtcgagcag gtcggcgagg atcgcgctcg tgagctcgcc gggcttcttc
       61 accgcgatgc cgtcggcgat cgtgaacccg cccggctcgc cgcccgcgag cggggcgcac
      121 gccgccgcct gcacgccgac gaggcggacg tccggccgca gcgcgcgcag cgcgatcgcg
      181 acacccgatg cgagcccgcc gccgccgacc gggacggcga cggcgccgag gtcctccggg
      241 agcccctccg cgagctcgag cccgagcgtc ccctggcctg cgatcacctc ggtgtcctcg
      301 aacgggtgga cgaacgtcgc gccgccctcg gcgcgctcct tgctcgcctc gtacgcctcg
      361 tcgtagcccg agccgcccat caccacctcc gcgccgtagc cgcgcgccgc ctcgaccttc
      421 gccatcggcg cgtcgtccgg gacgaagatc gtcgcgcgga tgcccgcctc gcgcgccgcc
      481 caggcgacgg cctggccgtg gttcccggcg ctcgcggtca cgaccccggc ctcccgctcc
      541 ccggggccga gcgtcgcgat cttgctcatc gccccgcgca ccttgaacga gccggtgcgc
      601 tggaggttct ccgccttgag gtagacgtcg cgccccgaca gccgcttgag cgtctcggac
      661 gcgaagacgg gcgtctcgcg cgccgacccg gtgagccgcc cgcgcgcttc ccggaggtcg
      721 tcgagctcag gtcgtctcat cgagccggaa cacctcctcg aggatctcgc gaacggcggt
      781 gagcgggtcg agccgccgcc ccttgacctc gtcgagcagc cgccggagct cggggtcgtc
      841 cgcgacggcg cgctcgaggt gcgtcttcgc gcgcccgctc gcgaccgcga agacctcgcc
      901 cgccagctgc tccccgcgcc gctcctcgag ccgcccgctc tcgacgaggt gccgccggtg
      961 gtttccgacc gcctcccaga gctcctcgat cccctcgccc cgcacggcct cggtgaggac
     1021 gatcggcggc gtccactcgc gcttctcgcc gagcccgagg atcgagcgaa cctcgttcat
     1081 catcgtcttc gccgccgggt ggtcgcgctt gttcacgacg atcacgtcgg gaatctccat
     1141 gatcccggcc ttgagcgcct ggatcgagtc gccggagccg ggcatgagcg cgaggacgat
     1201 cgtgtcggcg aggcccgtga tctcgacctc gctctggccg gtgcccacgg tctcgacgaa
     1261 gacgacgtcc ttgccgaacg cgtccaggag gagcgcggcc tggagcgtcg cctccgcgag
     1321 cccgccgagg tgcccgcgcg ttcccatcga gcggatgaag acgtcccggt cgaggaagtg
     1381 gtcggagagc cggatccggt cgccgaggag ggcgcccgag gtgaacgggc tcgacgggtc
     1441 gaccgagatg acgccgatcg tccgcccctc tccgcgcacg agccggacga gcgcgctcac
     1501 gaggctcgac ttcccgacgc cgggcgggcc cgtcatgccg acgatctcgg cccgacccgt
     1561 ctgcgggtag gtctcgcgga cgagctcgcc ggcgagcggg tcgccgttct cgacgaggct
     1621 gatcgcgcgc gcaagcgctc ggcgatcacc ggagcggagc ccctcgagga gcgactccgc
     1681 cgtccaggct gcgcgcgtgc tcaccgcgcc gctgcggcgg cggtgaggca ccgctcgagc
     1741 gcctcgccga agaggtcgtt ctcctccggc tcaccgaccg tgacgcgcat cgccgcgggc
     1801 gcgccgaacg ggccgagcgg gcggacgatc acgccctcgc gaaggagcgc gtcgaagagc
     1861 ggggcgacct cgcggccgca gtccacgtag acgaagttcg ccaccggcct cggacagatc
     1921 tccacgggga ggcgcgagca aacggcctcg agccgcgccc gcccctcggc cgtgacggcg
     1981 cggcgccgtg cgagctcctc cggctcgccg aggctcgcga gcgccgcgtc ctgcgccggc
     2041 tgggagacgt cgaacgcgtt cctcaccttg cggatcgccg cgacgacgtc cacgggcccc
     2101 accccgtagc cgacgcgaag cccggcaagc ccgtagatct tcgagaacgt cctcaggcag
     2161 aggacgcggc ggccgccgcg cttcaggtac tcctcgatgc cgtccgggta gtccggctcg
     2221 tccacgtact cgaagtacgc ctcgtcgagc acggtgagga cgtgctcggg aacggcgtcg
     2281 aagtaccggt cgagctccgc ccggccgacc atcgtgccgg tcgggttgtt cgggttcgcg
     2341 acgaagacga tcttcgtgcg cggcgtgacc gcggcgagga gcgcctcgag gtcgtacgtg
     2401 aggtcgcgga gcggcacgcg cacgggcgtg gcgcccatct tgatcgcgtc gatgacgtag
     2461 ctcgggaagg acggccaccc gcagacgacc tcgtcgcccg ggtcgagcgc ggcgagcgag
     2521 aggtacatga tcaccgcgtc cgcgccggcg gcgatcgcga cccgctccgg ctcgacgccg
     2581 tggcgctcgg cgagggcggt cgtgaggcgg tacatgccgc cgtccgggta gcggttcagc
     2641 tcgttgacgg agcgctggag cgcctcgacg gcggccggga acggcccgaa ctgcccctcg
     2701 ttcgaggcga gcttcacgac ccggtcgagg ccgagctcgc gctgcacctc ctcgaccggc
     2761 ttccccggct cgtacgggac cagatcggcg atcgccggcc ggaagagatc gccctgcgcc
     2821 atcggcgcaa ccctaaaggg tccgcgcgcg cgaccggcga ggtcggcggc gggctgacta
     2881 ggctgtccgg atggaggaga tcgggctctt tccgctgccg ctcgtcctcg tgccggggga
     2941 gcaggcgccg ctccacatct tcgagccgcg ctacaaggag ctcgtcgacg agtgcctcga
     3001 caccgcggcg ccgttcgggt tcctgctcgc ggacgacgac ggcgtgcgcg aggtggggac
     3061 gctcgccgag gtggtcgagg tcgtcgagcg cttcgacgac gggcgcctca acatcgtcgt
     3121 cgaaggggcg gcgcgcttcc gcgtcgcccg gctgaccgac ggacggtcgt tccagaccgc
     3181 ggaggtcgac acggtggacg acgaggggga cgagccgacc gacgacgagc gggcccgctg
     3241 cctcgctggc tacgacaagg tgatcgcggc cgcggcggcc gagctcgagg agctcgaccg
     3301 ggacgcggac gacctctcgt tccggatcgc ggcccggatc gacctcgggc cggagatcaa
     3361 gcagacgctc ctcgagagcc gctcggagcg ggagcgcgtc gtccggctcg ccgggctgct
     3421 cgaccgcgcg gccgaggcgg tcgcccgcga gcgcgaggtg cgcgagcgcg catccgggaa
     3481 cgggcgcgtc gaggcgctct agcgccgcgc tccggcgtgc gggcgcccgg cccgcggacg
     3541 aaccgacacg aaatcggcac ggtctcggca cggacacgtg acggcttcct cgatagcgtt
     3601 cgtgctggga ccgtcggtcg caggggcccc aatcacacgc gcgtgcgcgt gtacctggtc
     3661 gtggccgacg ggccgccgcg accgcggcgg ctcctggcgt tcacccgatc gagggacggc
     3721 aagcccgaac cggcgcgcga tccttcgcag agcgatgcct gcggccatcc agtctcggtt
     3781 cgtgctcgcg gcggcggccg tcgtcttcgc ggccggcttc gtcgcgttcg cgctcatccc
     3841 cacggacgac gtcgggatcc cgcacgtctt ctacatcgcg atcgcgctcg tcgccgccgt
     3901 caccgggccg ctctggggcg ccgcggccgg ggctctcgcg gcggcgctct actgcatcgg
     3961 gatgatcgtc cacccgatcg tcccgtccgc ggagatcctc accggcggca cgctcgtccg
     4021 cctcgccacc tacgcctgca cgggcgccct gatcggctgg ttcgcgcggg acaaccggcg
     4081 gctcgtcgcc gagcttcgga tcctcgccga ccgcgacacg gtcaccggcc tgccgaacac
     4141 ccgcgcgttc gaggcggcca tccagcgccg gctcgacgcc cagcggccct tcgcgctcct
     4201 gatcggcgac ctgcgcggcg tcgacgacgc gagcctcggc gaggacgtcg ccgcgaacga
     4261 cgcgctcctc cggatcggcg acgtgctcgg gaacgccgtc gggcccgggg acgacctcgc
     4321 gcgcgtcggc ggccgggcct tcgcgctcct cagctcgctc ggctccgcgc acgaggccgc
     4381 ccggcttgcc ggccgcctcg agcagacgct cgcggggcac ggcctcccca cgcgcttcgg
     4441 ctgggcggtg ttcccgcacg agggggcgaa cgcgctctcg ctttaccgcg ccgccgacga
     4501 gcggctctac gcccggaagc tcgtcagcgc cgacgcggcg gacgtcgggc tgcgctcggt
     4561 ctcctaagcg cgcccgcgca ccccgtgcac cgggcggccg gacgcctccc aggcgtaata
     4621 cgacgcgctc gggccgaact cgggccggta ctggacgacc cgcgggttcc ggccgacctc
     4681 cttcgcacgc tgcgcatgcc ggaggaggtt cggatcgtcc cgctcctccc acacgaggat
     4741 caggtgcgcc gccgtcatga acgacgcgac gaagtcgcag cccgcggccg ggcacgggaa
     4801 cgggtcggtg gcgacgcagt accagccggt gtggtcggtg ccctcgtcgc cggtccacag
     4861 ctcttcctcc tcgacgcgct cttcctcctc cgtgaggagc gtcccgcggc ccggctcgag
     4921 cggctccgtc attgcgcgcg gatcacgtgg ccgaactcgt cgagctcgac gaagtgctcg
     4981 gccgtgccgt cgcggtaggc ggagcagcgc tcggcggcgt tccggtcctc gtcgcgcggc
     5041 tcgcggacga ccacgaccac ccagtcctcg cggtcgggaa gcgggtcagc gatgcacggc
     5101 ccgtcggaga agtcgcgccc gctctcctcc tcggcagcgt aggcgtcccg cgcgtcgttc
     5161 ag
//
LOCUS       DASTFC010000167         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1552294,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000167 DASTFC010000000
VERSION     DASTFC010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552294"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFO88_05045"
     CDS             complement(<1..401)
                     /locus_tag="VFO88_05045"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET9544921.1"
                     /translation="MTETATGERLAELIGGRQPVVVLTGAGVSTESGIPDFRSPTGIW
                     AEFDPREYATLGAFHDDPEKVWRFYAPRFAMLGEARPNRVHRALAELERMGLVRAVVT
                     QNIDLLHERAGSRDVVEVHGSIRVSHCLACGA"
     gene            616..978
                     /locus_tag="VFO88_05050"
     CDS             616..978
                     /locus_tag="VFO88_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544922.1"
                     /translation="MTLAMMVGMVASAAVFLATVGLTVDEALRRHAALFVLAQAFGMT
                     VAMVVWMRHRGHGRRSCSEMAAAMVVPALVLIALRTLDVISGPICGVYCMSTLAAMVL
                     VMLSRPSEYGRWAPPVPR"
     gene            complement(1016..1789)
                     /locus_tag="VFO88_05055"
     CDS             complement(1016..1789)
                     /locus_tag="VFO88_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544923.1"
                     /translation="MRPTGLVLVLLAALAGGCGGDAEEAAPPSTVTVTVEQTTETAPA
                     TTEPADDPCAPEDVAAAVERGLGEWVARVELVRCRNGHARASAFPEEGEMLETADVLL
                     RLVGDAWEIVDYGTSIGCEEASLPPARAANRRACAALGYPQPAILTGASFQLPSRNIG
                     CRAGGGALRCDVLTGLRPEPGAPCELDWVGVVMEPRGPAEPLCAGDTAYDASAQTLAY
                     GDLWHRGPFWCRSSESGLLCVNRDLEGSFELARERWSAG"
     gene            1944..2666
                     /locus_tag="VFO88_05060"
     CDS             1944..2666
                     /locus_tag="VFO88_05060"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET9544924.1"
                     /translation="MAADNAEPQPDQYVAYTLYRLDPAWRRLPVEERSAHKDAFAEVV
                     EAWAERMDVRTYSLVGVRPEADFMFWKLTERFEDLRELATELNGTPLAGWLETPYSYL
                     AATRPSKYFERDPRRPPRRVIPRGAPYLVVYPFVKIRPWYALSMEERGHAMREHAEVG
                     ARFVTITNHTTYSFGIDDQEFMTAFECEAPSDFLELMMTLRETEASRYTERDTPIFVG
                     EALPLREILDSLDGAKARIEAR"
     gene            complement(2663..3052)
                     /locus_tag="VFO88_05065"
     CDS             complement(2663..3052)
                     /locus_tag="VFO88_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544925.1"
                     /translation="MTDWDPRTNGTLRGFLIILAVAALVTAAGRGGAIALGTFLLILR
                     IAFIVVIAIVLFRLWRSRREEIAEWPRRARVVFYGAAAVAFANVLANFFTTYPAGALE
                     ALVFFGVLGACAYALVRTWRDQHTYGY"
     gene            complement(3049..>3312)
                     /locus_tag="VFO88_05070"
     CDS             complement(3049..>3312)
                     /locus_tag="VFO88_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544926.1"
                     /translation="RTPGRVYAHVLAGAQALVIAQVALGLLLLSDNLRTADRLHYSYG
                     VLALLAVLSPWLYAPPVPARRLLWFAGASLVAGALSIRAYTTG"
BASE COUNT          500 a         1201 c         1176 g          435 t
ORIGIN      
        1 gcgccgcacg cgagacagtg cgagacccgg atcgagccgt gcacctcgac gacgtcgcgg
       61 ctcccggcgc gctcgtgcag gaggtcgatg ttctgcgtga cgaccgcccg cacgagcccc
      121 atccgctcga gctccgcgag cgcccggtgc actcggttcg gccgggcctc gccgagcatc
      181 gcgaagcgtg gcgcgtagaa gcgccagacc ttctcgggat cgtcgtggaa cgccccgagc
      241 gtcgcgtact cccgcgggtc gaactcggcc cagatcccgg tcggcgagcg aaagtccggg
      301 atcccgctct cggtcgagac gccggcaccg gtcagcacga cgaccggctg ccgccccccg
      361 atcagctcgg cgagacgctc gcctgtcgcc gtttcggtca tgaccgccgc aagtctgcct
      421 ctccgggccg gcgcgagcag cgtggggagt ttctacgcac ttgattacgt gccgtctcgg
      481 atggcccgcc ctcgtggcgc atgcaacgtt ccaggccaag cgagaggaga agcgatgaac
      541 atcagcactg caatccaccg gggacacaca ggtcgcggtg cacgcaactt ccacttcctt
      601 cgccatttgg tcgagatgac gctggcgatg atggtcggga tggtcgcgag cgcagccgtc
      661 tttctcgcga cggtcggcct gacggtcgac gaggcgctcc gccggcacgc ggcgctgttc
      721 gtgctcgcgc aagcattcgg catgacggtc gcgatggtcg tctggatgcg ccaccgcggg
      781 cacgggcggc gaagctgttc cgagatggcg gcggcgatgg tcgttcccgc gctcgtcctc
      841 atcgccctcc gcacgctcga tgtcatcagc ggaccgatct gcggggtgta ctgcatgtcg
      901 acgctcgcgg cgatggtcct cgtgatgctt tcccggccga gcgagtacgg acgatgggca
      961 cctcccgtcc cgcgctgacc cgcacgggcg gcgagacccg cggctgttgc ccggcctagc
     1021 ctgcgctcca gcgttcgcgg gcgagctcga atgagccctc gaggtcgcgg ttcacgcaga
     1081 ggagcccgga ctcgctcgat cggcaccaga acgggccgcg gtgccagagg tcgccgtacg
     1141 cgagcgtctg cgcggaggcg tcgtacgcgg tgtcgccggc gcagagcggc tcggccggcc
     1201 ccctcggctc catgacgacg ccgacccagt cgagctcaca cggtgcgccc ggctcggggc
     1261 gcaggccggt gaggacgtcg cagcgcagcg ctccgccgcc cgcccggcag ccgatgttcc
     1321 gcgacggcag ctggaacgac gcaccggtga ggatcgccgg ctgcgggtag ccgagcgcgg
     1381 cgcaggctct ccggttggcg gcccgtgcag gcggcaggga cgcctcctcg cagccgatcg
     1441 aggtcccgta gtcgacgatc tcccaggcgt cgccgacgag ccggaggagc acgtcggccg
     1501 tctcgagcat ctcgccctcc tccgggaatg ccgaggcccg cgcgtggccg ttgcggcagc
     1561 ggacgagctc gacccgcgcg acccactccc cgagcccacg ctccaccgcc gcggccacgt
     1621 cctccggagc gcacggatcg tccgcaggct ccgtcgtcgc cggcgcggtc tccgtcgtct
     1681 gctcgaccgt gaccgtcacc gtcgacggcg gcgccgcctc ctcggcatcg ccgccgcagc
     1741 cgccggcgag cgccgcgaga aggacgagaa cgagcccggt cgggcgcacg gcgccagcgt
     1801 accgccgcag ccgggaggtc gcggggcgcg tcccccggcc cgctcggtgg acgcgccccg
     1861 acgccatcca cgctatgccg gaaaagcgca caagtgacgc gcgaaacgtc gagagagcgc
     1921 aacggcgccg ttacgatttc gccatggccg ccgacaacgc cgagccgcag cccgaccagt
     1981 acgtcgcgta cacgctctac cggctcgacc ctgcgtggcg tcggctccct gtggaggagc
     2041 ggagcgcgca caaggacgcg ttcgcagagg tcgtcgaggc ctgggccgag cggatggacg
     2101 tccgcacgta cagcctcgtc ggcgtccggc ccgaggcgga cttcatgttc tggaagctga
     2161 ccgagcgctt cgaggatctc cgcgagctcg cgaccgagct caacgggacg ccgctggcgg
     2221 gctggctcga gacgccgtac agctacctcg ccgcgacccg cccgtcgaag tacttcgagc
     2281 gcgacccgcg ccgcccgccg cgccgcgtga tcccgcgcgg ggcgccgtac ctcgtcgtct
     2341 atccgttcgt gaagatccgc ccctggtacg cgctctccat ggaggagcgc ggccacgcga
     2401 tgcgcgagca cgcggaggtc ggcgcgcgct tcgtcacgat cacgaaccac acgacgtact
     2461 ccttcgggat cgacgaccag gagttcatga ccgcgttcga gtgcgaggcg ccgtccgact
     2521 tcctcgagct gatgatgacc ctgcgcgaga ccgaggcgag ccgctacacc gagcgcgaca
     2581 cgccgatctt cgtcggcgag gcgcttccgc tccgcgagat cctcgactcg ctcgacggcg
     2641 cgaaggcccg aatcgaggcg cgctagtagc cgtacgtgtg ctggtcgcgc cacgtgcgca
     2701 cgagcgcgta ggcgcaggcg ccgagcacgc cgaagaagac gagcgcctcg agcgcgcccg
     2761 ccgggtacgt cgtgaagaag ttcgcaagca cgttcgcgaa cgcgaccgcc gcggcgccgt
     2821 agaacacgac gcgggcacgg cgcggccatt cggcgatctc ctcccgccgc gagcgccaca
     2881 ggcggaagag gacgatcgcg atcacgacga tgaacgcgat gcgcaggatc agcaggaacg
     2941 tcccgagggc gatcgcccct ccccgccccg ccgcggtgac gagcgcggcg acggcgagga
     3001 tgatgaggaa gccgcggagc gtgccgttcg tgcggggatc ccagtcggtc atccggtcgt
     3061 gtacgcgcgg atcgagagcg cgcccgcgac gaggctcgcg cccgcgaacc agaggagtct
     3121 ccgcgcgggg acgggcggcg cgtagagcca cggcgagagc accgcgagga gcgcgagcac
     3181 cccgtacgag tagtggagcc ggtcggcggt gcggaggttg tccgagagga gcagcaggcc
     3241 gagcgcgacc tgcgcgatca cgagcgcctg cgcgccggcg agcacgtgcg cgtagacccg
     3301 ccccggcgtc cg
//
LOCUS       DASTFC010000168         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_217418,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000168 DASTFC010000000
VERSION     DASTFC010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217418"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFO88_05075"
     CDS             complement(<1..771)
                     /locus_tag="VFO88_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9544927.1"
                     /translation="MSDAPAERAEGTQAMSVRGAAFLGVGAMVGAGIFALLGEAGAVA
                     GAAVWLSFLIAGIVAALLGYTVVKLGVRYPSSGGLIAYLVQGFGNGRLVGIASWLGYA
                     AAIVIVCSMVAVSFGSYATSLFVGDDAGSGWDNLFTSAIVVAAAVVSIVGASFVDRAQ
                     TLIVFLLLAVFAVFIAVTITQIDLDLLAFDGYPPFSDIVSSVALTFFAYLGFSVITFA
                     AGDMRDPSRELPQAMYRALGVTTFLYLLIAISVFGTLTV"
     gene            complement(779..2122)
                     /locus_tag="VFO88_05080"
     CDS             complement(779..2122)
                     /locus_tag="VFO88_05080"
                     /inference="COORDINATES: protein motif:HMM:NF006927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
                     /protein_id="HET9544928.1"
                     /translation="MTTADWILLALEGLVVIGFIALGVRAGGVGLGIWGGVGTLVLVF
                     LFGLEPGEPPVSAMLIIITVITATAAMQAAGGIDYMVQIASHALRRSPRAINFVAPYV
                     SWLLTVLTGTGNTFFSLIPVINEVSYANKIRPERALAGSAVASTFGITASPVAAAMAT
                     MLPLVEVYDYELVDVLLITVPASLVGILAMAVVMNGRGPELEDDAEYQRRLAAGEVHP
                     PAPVADIELMRFAKRSVGVFLAAVLAICLFGFFEDLRPTVAVEGGGTEPMSITPIIGM
                     VMLTGAALMLLLAAVKPGEIVDAPIFKSGMVAMIALFGIAWMADTFIAANEDAIVTAL
                     GSLAENWPFMIAVAIFLVAALTTSQSAATRTMVPLGLALGIGPQYMIAMWTAVGGVLF
                     FPANGTQIAAAQQDETGTTTLGKRVVDHSFQLPLQIAWIVTALVGIGIAWLFFGE"
     gene            complement(2119..>2413)
                     /locus_tag="VFO88_05085"
     CDS             complement(2119..>2413)
                     /locus_tag="VFO88_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544929.1"
                     /translation="ESAGVPETPEGQQAADEIQAWADTAHTNLEDSLDAFEDTGDEPR
                     ERLEALGAATGGVLETLVTGVETLVGAARLDAQLGAAFVDAETCRSFGEEEGA"
BASE COUNT          406 a          842 c          848 g          317 t
ORIGIN      
        1 caccgtgagc gtcccgaaga ccgagatcgc aatcagcagg tacaggaacg ttgtcacgcc
       61 gagcgcgcgg tacatcgcct gcggcagctc tcgggacggg tcgcgcatgt cgccggccgc
      121 gaacgtgatc acgctgaagc cgaggtaggc gaagaacgtg agggcgacgc tcgagacgat
      181 gtccgaaaac ggcgggtagc catcgaaggc gagcaggtcg aggtcgatct gcgtgatcgt
      241 gaccgcgatg aagaccgcga agaccgcgag caggaggaag acgatgagcg tctgcgcgcg
      301 gtcgacgaac gacgcgccga cgatgctgac gaccgcggcc gcgacgacga tcgccgaggt
      361 gaagaggttg tcccacccgc tccccgcgtc gtcgcccacg aagagcgacg tggcgtagct
      421 tccgaacgag accgccacca tcgagcagac gatcacgatc gcggccgcgt agcccagcca
      481 ggacgcgatc ccgacgagcc tcccgttccc gaagccctgg acgaggtacg cgatcaggcc
      541 gcccgaggac ggatagcgga cgccgagctt gaccaccgtg tagccgagca gggcggcgac
      601 gatccccgca atcaggaacg agagccagac cgcggcgccg gcgaccgcgc cggcctcgcc
      661 gaggagcgcg aagatccccg ctcccaccat cgcgccgacg ccgagaaacg ctgcgccgcg
      721 cacgctcatt gcctgcgtcc cctcggcgcg ttcggccggc gcgtcgctca cgggccgtct
      781 actcgccgaa gaacagccat gcgatgccga ttcccacgag cgccgtcacg atccacgcga
      841 tctggagcgg gagctggaac gagtggtcga cgactcgctt cccgagcgtc gtcgtgccgg
      901 tctcgtcctg ctgagccgcc gcgatctggg tgccgttcgc cgggaagaag aggacgccgc
      961 cgaccgccgt ccacatggcg atcatgtact gcggcccgat gccgagcgcg agcccgagcg
     1021 gcaccatggt gcgcgtcgcg gccgactggc tcgtggtgag agcggcgacg aggaagatcg
     1081 cgaccgcgat catgaacggc cagttctccg cgaggctgcc gagcgccgtc acgatcgcgt
     1141 cctcgttggc ggcgatgaac gtgtcggcca tccaggcgat gccgaacagg gcgatcatcg
     1201 ccaccatccc cgacttgaag atcggcgcgt cgacgatctc gcccggcttg accgccgcga
     1261 gcaggagcat gagcgccgcg ccggtgagca tgaccatccc gatgatcggc gtgatcgaca
     1321 tgggctccgt gccgccgccc tcgaccgcca cggtggggcg gaggtcctcg aagaagccga
     1381 agaggcagat cgcgagcacg gcggcgagga agacgcccac gctccgcttg gcgaagcgca
     1441 tgagctcgat gtcggcgacg ggcgccggcg gatggacttc gcccgccgcg agccggcgct
     1501 ggtactcggc gtcgtcctcg agctcgggcc cgcgcccgtt catgacgacc gccatggcga
     1561 ggatcccgac gaggctcgca gggaccgtga tgagcaggac gtcgacgagc tcgtagtcgt
     1621 acacctcgac gagcgggagc atcgtcgcca tggcggccgc gaccgggctt gccgtgatcc
     1681 cgaacgtcga ggcgacggcc gagccggcga gggcccgctc gggccggatc ttgttcgcgt
     1741 agctcacctc gttgatgacc gggatgagcg agaagaacgt gtttccggtc ccggtcagga
     1801 cggtcaagag ccacgagacg tacggcgcga cgaagttgat ggcgcgcggg ctgcgccgga
     1861 gcgcgtggct cgcgatctgg accatgtagt cgatccctcc ggccgcctgc atcgcggcgg
     1921 tcgccgtgat gaccgtgatg atgatcagca tcgcgctgac cggcggctcc cccggctcga
     1981 ggccgaacag gaagacgagg acgagcgtgc ccacgccgcc ccagattccg aggccgaccc
     2041 cgccggcgcg cacgccgagc gcgatgaagc cgatgacgac cagcccttcg agcgcgagga
     2101 ggatccagtc ggcggtcgtc acgcgccctc ctcctccccg aagctcctgc aggtctcggc
     2161 gtcgacgaac gccgccccga gctgggcgtc gaggcgggcc gcgccgacga gcgtctcgac
     2221 gccggtcacg agcgtctcga ggactccgcc ggtggcggct cccagcgcct cgagcctctc
     2281 ccgcggctcg tcgcccgtgt cctcgaaggc gtcgagcgag tcctcgaggt tcgtgtgcgc
     2341 ggtgtccgcc cacgcctgga tctcgtcggc cgcctgctgg ccctcgggcg tctcgggcac
     2401 gcccgcgctc tcg
//
LOCUS       DASTFC010000169         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1156788,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000169 DASTFC010000000
VERSION     DASTFC010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156788"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VFO88_05090"
     CDS             complement(<1..1103)
                     /locus_tag="VFO88_05090"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET9544930.1"
                     /translation="MGRFARSREDGHEQRATTLELFYDLVFVFAITQVSHLLLDNLTF
                     QGMGQAAIVLFVVWWAWNYTTWVTNELDPDSPAVRLLLIGVMLASLFMAVAIPEAFGE
                     RGLLFAASYVAIQVGRHTFLAFVAGDPGSLERARAARILVWFVVAGAFWLAGGLADGS
                     ERTALWLAGIAIEVAGPFTVYWLPGRPRLPHSTWNVETTHFAERFQLFMIIALGETIV
                     ITGATAAGLEIDTPRVAAFAFAFLATAAMWWLYFTYVARIAERRLAQAPVDERTRLAR
                     DGYTYLHVVMVAGIIVAAVGDELVIAHPLDVLPGREVAVVVAGPALYLLAHVLFRLRM
                     ARSLSWKRLGGALACVALAPLGLVAPALVLSALV"
     gene            complement(1183..2970)
                     /locus_tag="VFO88_05095"
     CDS             complement(1183..2970)
                     /locus_tag="VFO88_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544931.1"
                     /translation="MAERSRGARASRRRTTWGIAVFAALALVMVALPAAATAQLPNPD
                     DPRVGLDPGFQNAEVASHGVDLTAHRDRPAGFFLEGNPGSFGFLNSDLAIQGDVAFVG
                     GFRGWHAWNIADPSNPTLEVGVVCPGGQGDPSVWGNLLFLSYENPSRIDCGTQAETAD
                     TAFRGVRIFDISDLGNPVQVAAVQTCRGSHTHTLVTDPNDAENIYVYNSGTSGVRSAT
                     HTGRPANCFENGQPVPEASRWAIDVIKVPLAAPQNAAIVNQPRIFANPDTGAINGLQN
                     TFPTPLHPSGIAWSPQPITDACHDITAYPEIGLAAGACEGNGILIDISDPANPVRVDA
                     VSDPNFAYWHSATFSNDGTKVVFTDEWGGGTSARCRSTDRLNWGANAIFDIVDGKMEF
                     RSYYKMPAVQTSQENCVAHNGNLIPVPGRDILSQAWYQGGHSVMDFTDSSNPKEIAYY
                     DRGPVSGTSLVLGGFWSTYFYNGTTFATELSRGFDVFDYTPTDDLSANEIQAASEPQV
                     DRLNAQLQSEIVWAPSFAVVRSHLDQLIRAGAIGGETLDEVEKHLAMAEEATQGKKAK
                     GQLTAAADKLEGEQYDALRGALLDLAATY"
     gene            3160..3852
                     /locus_tag="VFO88_05100"
     CDS             3160..3852
                     /locus_tag="VFO88_05100"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET9544932.1"
                     /translation="MEKTSPSARRLAAVALVALLGLAGCSSGNGSGDAATTAAETDTA
                     PRVVQPGAPGEGVRELTPEELEDLEPLPHTAADVQFMQYMILHHRQALRMTDLVPDRS
                     RRGDIPLLARRIEISQEAELEQMIAWLEERDAAVPPPGGEEAHAGHLPGMLTEADLER
                     LEASEGPRFDRRFLAAMIRHHEGALLMVQQLQEVPGAGQEPEIAVFAGHVVADQEIEI
                     RRMETLLRELAR"
BASE COUNT          597 a         1402 c         1439 g          556 t
ORIGIN      
        1 acgagcgcgg agaggacgag cgccggcgcc acgaggccga gcggcgcgag cgcgacgcac
       61 gcgagcgcgc cgccgagccg cttccacgag agcgaccggg ccatgcggag ccggaagagc
      121 acgtgcgcga gcaggtagag cgccggcccg gcgacgacga ccgccacctc ccggccgggc
      181 agcacgtcga gcggatgggc gatcacgagc tcgtcgccga cggcggccac gatgatcccg
      241 gccaccatca cgacgtggag gtacgtgtag ccgtcgcgcg cgagccgcgt cctctcgtcc
      301 acgggcgcct gcgcgagccg ccgctccgcg atgcgcgcga cgtacgtgaa gtagagccac
      361 cacatcgcgg ccgtcgcgag gaacgcgaat gcgaacgcgg cgacccgcgg ggtgtcgatc
      421 tcgagccccg cggccgtcgc ccccgtgatc acgatcgtct cgccgagcgc gatgatcatg
      481 aagagctgga aacgctcggc gaagtgcgtc gtctcgacgt tccaggtcga gtgcgggagc
      541 cgcgggcggc ccgggagcca gtagaccgtg aacggcccgg cgacctcgat cgcgatcccc
      601 gcgagccaga gcgccgttcg ctcggagccg tcggcgaggc cgcccgccag ccagaacgct
      661 cccgccacga cgaaccagac gaggatccgc gccgcacggg cgcgctcgag gctgcccggg
      721 tcgccggcga cgaaggcgag gaacgtgtgc cgccccacct ggatcgccac gtacgacgcg
      781 gcgaagagga gcccgcgctc gccgaacgcc tccgggatcg cgaccgccat gaagaggctc
      841 gcgagcatca cgccgatgag aaggaggcgg acggccggcg agtccgggtc gagctcgttc
      901 gtaacccagg tcgtgtagtt ccacgcccac cagacgacga agaggacgat cgccgcctgg
      961 cccatcccct ggaaggtcag gttgtcgagc agcaggtgcg agacctgcgt gatcgcgaag
     1021 acgaagacga ggtcgtagaa gagctcgagc gtcgtcgccc gctgctcgtg cccgtcctcg
     1081 cgcgaccgcg cgaacctgcc catccgcgaa acggtagagc aacgcgagag gggcgccgct
     1141 cgggcgcccc tctcggtcgt tctcgtttcg ggcccggccg tgctagtacg tcgcggccag
     1201 gtcgagcagc gctccgcgga gcgcgtcgta ctgctcgccc tcgagcttgt cggcggcggc
     1261 cgtgagctgg cccttcgcct tcttgccctg cgtggcctcc tcggccatgg cgaggtgctt
     1321 ctcgacctcg tcgagggtct cgccgccgat cgcacccgcg cggatcagct gatccaggtg
     1381 cgagcggacg accgcgaagc tcggagccca gacgatctcg gactggagct gcgcgttcag
     1441 ccggtcgacc tgcggctccg acgcggcctg gatctcgttc gcggagagat cgtcggtcgg
     1501 cgtgtagtcg aacacgtcga acccacgcga gagctcggtc gcgaacgtcg tcccgttgta
     1561 gaagtacgtc gaccagaagc cgccgaggac gagcgaggtc ccgctaacag gcccgcggtc
     1621 gtagtaggcg atctccttcg ggttcgacga gtcggtgaag tccatcaccg agtggccgcc
     1681 ctggtaccag gcctggctca ggatgtcgcg gcccggcacg gggatcaggt tcccgttgtg
     1741 ggccacgcag ttctcctggc tcgtctgcac cgcgggcatc ttgtagtagc tacggaactc
     1801 catcttcccg tcgacgatgt cgaagatggc gttcgcgccc cagttcagcc tgtcggtgct
     1861 ccggcagcgc gcgctcgtgc cgccgcccca ctcgtccgtg aagacgacct tcgtcccgtc
     1921 gttcgagaac gtcgccgagt gccagtaggc gaagttcggg tcggagaccg cgtcgacgcg
     1981 gaccgggttc gccgggtcgc tgatgtcgat caggatcccg ttgccctcac acgcgccggc
     2041 cgcgagcccg atctccgggt acgcggtgat gtcgtggcac gcatccgtga tcggctgcgg
     2101 gctccaggcg atccccgaag gatggagcgg cgtcgggaag gtgttctgga gaccgttgat
     2161 cgcgcccgtg tccgggttcg cgaagatccg cggctggttc acgatcgccg cgttctgcgg
     2221 ggcggcgagc gggaccttga tcacgtcgat cgcccagcgg ctcgcctcgg ggacgggctg
     2281 cccgttctcg aagcagttcg ccggtcggcc ggtgtgcgtc gccgagcgga cgcccgaggt
     2341 gcccgagttg tagacgtaga tgttctccgc gtcgttcggg tccgtcacga gcgtgtgcgt
     2401 gtgcgagccg cggcacgtct ggacggccgc gacctgcacc gggttgccga ggtcggagat
     2461 gtcgaagatc cggacgccgc ggaacgcggt gtcggccgtc tccgcctgcg tcccgcagtc
     2521 gatccgcgac gggttctcgt acgagaggaa caggaggttc ccccacacgg agggatcgcc
     2581 ctggccgccc gggcagacga cgccgacctc gagggtcggg ttgctcgggt cggcgatgtt
     2641 ccacgcgtgc cagccgcgga agccgccgac gaacgcgacg tcgccctgga tcgcgagatc
     2701 cgagttgagg aagccgaagc tccccgggtt gccctccagg aagaagccgg cgggccggtc
     2761 acggtgcgcc gtcaggtcga cgccgtgcga ggcgacctcg gcgttctgga agccggggtc
     2821 gagcccgacg cgcgggtcgt ccgggttggg aagctgcgcc gtcgcggcgg cggggagcgc
     2881 caccatgacg agcgcgagcg ccgcgaagac cgcgatgccc cacgtggtac ggcgccggga
     2941 tgcccgcgcg cctcgagaac gctctgccat aaatccctcc tgttcgtgtc cccgtcgtcc
     3001 cgcgtcgcgg ttccgcaccc ggcgtacccg gcctcaattc gtcgcgggag cggccaccat
     3061 aaccgcagtt tcgtagtacc gcaagagttg acataagtgt gcggtttgcg gggaaatctt
     3121 ggccgcgcgg gctcgagcgg tttctggcac gctgcagaaa tggagaaaac ctccccctcg
     3181 gcgcggcgcc tcgcggcggt cgccctcgtc gccctcctcg gcctggccgg ctgcagctcg
     3241 ggcaacggct ccggggacgc ggcgaccacc gcggcggaga cggacacggc cccgcgggtc
     3301 gtccagccgg gcgcgcccgg cgagggcgtg cgcgagctga cgccggagga gctcgaggac
     3361 ctggagccgc tgccgcacac ggcggcggac gtccagttca tgcagtacat gatcctccac
     3421 caccgccagg cgctccgcat gaccgacctc gtccccgacc gctcgcggcg cggggacatc
     3481 ccgctcctcg cccgccgcat cgagatctcc caggaggccg agctcgagca gatgatcgcc
     3541 tggctcgagg agcgcgacgc ggccgttccc ccgcccggcg gcgaggaggc acacgccggg
     3601 cacctccccg ggatgctcac ggaggccgat ctcgagcggc tcgaggcctc ggaggggccg
     3661 cggttcgacc ggcggttcct cgcggcgatg atccgccacc acgagggcgc cctcctgatg
     3721 gtgcagcagc tccaggaggt gccgggcgcc gggcaggagc ccgagatcgc cgtgtttgca
     3781 ggccacgtcg tcgcggatca ggagatcgag atccgccgga tggagacgct cctgcgcgag
     3841 ctcgcccgct gacgcaggtg cccgccgcgg cggcgggcag gcattgcgcg gccgcctagc
     3901 ccacctcctc gcgctcggcc cgctcgcgcc ggtcgtcgac gaagaacgcg ccgatcgtgc
     3961 cggcgagcga ggcgacgacc acgaggccga agac
//
LOCUS       DASTFC010000170         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_860345,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000170 DASTFC010000000
VERSION     DASTFC010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860345"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VFO88_05105"
     CDS             complement(<1..460)
                     /locus_tag="VFO88_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9544933.1"
                     /translation="MKVLLTGSHGYLGSVTGRVLADAGHEVVGLDARLYEGCDLRGAG
                     EGGARTALVRDVRDVTASDLAGVDAVVHLAALSNDPIGELSPELTRAINFEASVSLAR
                     AARESGASRFVFASSCSMYGASDTDELVDEQAPLRPLTAYAESKVLAEEAL"
     gene            complement(485..1900)
                     /locus_tag="VFO88_05110"
     CDS             complement(485..1900)
                     /locus_tag="VFO88_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HET9544934.1"
                     /translation="MRQGLSLLACALCAALFAVPAAEAAVKGPLRVLESNPRYFTDGT
                     GKAVYLTGSHVWWNLGAPDWTSGCWDAPAAFDWDRHLDWLEDHDHNFVRLWRVEHTRW
                     TMCFDDLGNPRQTTTAFHPWPRTGPGTAHDGGPKFDLGELDQAYFDRLRARVEAANEH
                     GIYVSVMLFEGWWLHSVDPASRWVFHPFHADNNVNGIDGDVDDDGLGTEIVTLAPTNA
                     AILAIQEAYVEKVVETVNDLPNVLYEIANEAGAWSTQWQYHMIRFVKGLQPAGQKRPV
                     GMTYPHLGGNNKLVFRSPADWVSPWAHRYMTDPPPADGRKVVVSDTDHHCGICGPSDA
                     VQADASFAWRSLTRGLNPIYMDVIDEGAYDPVRTPIRDALGQTLRWSKRIDLAASRPR
                     GHVSSTGYALTVGRRQYLVYQPRPRAFRVDLRKATGRFRVEWYRPAVGRSTLGKRVRG
                     GAVRRFTPPFKGSAVLFLRRL"
     gene            complement(1914..2822)
                     /locus_tag="VFO88_05115"
     CDS             complement(1914..2822)
                     /locus_tag="VFO88_05115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9544935.1"
                     /translation="MITAVVLNWRTPDLTIRCVRSLADDGVPLERIVVVDNGSADDSF
                     ARFGEELRGAVLVRLEENVGFARGNNIGARALPAAAYLFVNSDAFVDRPGSVERMRRA
                     LDDPRVGVVAPRLVNEDGSLQPTVVPLTTPAVAAVRASGLSRLVPNRFQPRWSTHWDH
                     SESREIQAANGAVLLVRRETFDALGGFDESSHMYAEDLDLCWRARRLGRTVRFVHDAQ
                     FVHLGAGSSGGDERDPARAERVAAAEAAMLARNLSPARARLASGLVVAGLAARAAAFR
                     LLRRPAAAAEMSASLRGLRTARRRER"
     gene            complement(2819..3523)
                     /locus_tag="VFO88_05120"
     CDS             complement(2819..3523)
                     /locus_tag="VFO88_05120"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HET9544936.1"
                     /translation="MIDTHCHLLPGLDDGPKTREDALDLAGALLADGIDRVLCTPHYA
                     SQFPTRHDDAEERLRELRPELEALGLEASLAAEVSPGHAVSAPLEELRRRAIGGRYLL
                     VEVLRDTPPSFFPSVFARLEEIELLPLFGHPERCRAVQRSARIADHILRDGGVLQVVA
                     PSLIGRFGGDAAAAGWRLVDTGRAGLLASDSHGTRRRSPHLGDAARMVADRLGDAAAA
                     ELTEHAPARLLAGERP"
     gene            complement(3520..>4444)
                     /locus_tag="VFO88_05125"
     CDS             complement(3520..>4444)
                     /locus_tag="VFO88_05125"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9544937.1"
                     /translation="AVALAGSAFHDARSGPARLAGEQLAAASRRADLLHFFDLTGPLL
                     RPRRRFTTTIHDASIVHGFEKVRHAYKRRVYPWALGRAARVVAVSHFARDEARRHFGT
                     PPERVTVVRSGPGLVQADGAAPADGLPPCPFLLYVGDLTAKKDVATLVRAYARSGVDD
                     GLVLVGRPREPYPELDAALAAAGPRVTVLEGAPDSTLVALYGAATALLLPSRYEGFGF
                     TPLEGMAHGCPVLASDIPAVREISGDGALLAPVGDEDAWADGIRRIARDEALRADLRL
                     RGAETVARYSWEETARGLCRVFESLRPGARA"
BASE COUNT          594 a         1587 c         1734 g          529 t
ORIGIN      
        1 cgagcgcctc ctcggcgagc accttcgact ccgcgtacgc ggtgagcggc cggagcggcg
       61 cctgctcgtc cacgagctcg tccgtgtccg acgcgccgta catcgagcac gacgaggcga
      121 agacgaagcg cgacgcgccc gactcccgcg ccgcgcgggc gagcgagacg gaggcctcga
      181 agttgatcgc gcgcgtgagc tcgggcgaga gctcgccgat cgggtcgttc gagagcgccg
      241 cgaggtggac gaccgcgtcg accccggcga ggtcggacgc ggtcacgtcg cgcacgtcgc
      301 gcacgagcgc cgtccgcgcg ccgccctcgc cggcgccgcg caggtcgcag ccctcgtaga
      361 gccgcgcgtc gagccccacg acctcgtgcc ccgcgtccgc gagcacccgc cccgtgaccg
      421 agccgaggta gccgtggctg ccggtgagga ggaccttcat cgccgtacga actatgcgcg
      481 accgctagag gcgccgcagg aagagcacgg ccgatccctt gaacgggggc gtgaagcgcc
      541 gtaccgcgcc gccgcgcacc cgcttcccaa gcgtcgagcg cccgaccgcc gggcggtacc
      601 actcgacgcg gaagcgcccg gtcgccttgc gcaggtcgac gcggaaggcg cgcggcctcg
      661 gctggtacac gaggtactgc cggcggccca cggtgagcgc gtagccggtg gacgacacgt
      721 ggccgcgcgg ccgcgaggcg gcgaggtcga tccgcttcga ccagcggagc gtctggccga
      781 gggcgtcccg gatcggcgtc cggacggggt cgtacgcgcc ctcgtcgatc acgtccatgt
      841 agatcgggtt cagcccgcgc gtgaggctcc gccacgcgaa cgacgcgtcg gcctgcaccg
      901 cgtcggacgg gccgcagatc ccgcagtggt ggtcggtgtc cgagacgacc accttgcgcc
      961 cgtcggccgg cggcgggtcg gtcatgtacc ggtgggccca aggggagacc cagtcggccg
     1021 ggctccggaa gacgagcttg ttgttgccgc cgaggtgcgg atacgtcatg ccgacggggc
     1081 gcttctggcc ggccggctgg aggcccttca cgaagcggat catgtggtac tgccattgcg
     1141 tcgaccacgc gccggcctcg ttcgctatct cgtagaggac gttcgggagg tcgttcacgg
     1201 tctcgacgac cttctcgacg tacgcctcct ggatcgcgag gatcgcggcg ttcgtcggcg
     1261 caagcgtgac gatctccgtc ccgaggccgt cgtcgtccac gtcgccgtcg atcccgttca
     1321 cgttgttgtc cgcgtggaac gggtggaaga cccagcgcga ggcggggtcg acggagtgga
     1381 gccaccagcc ctcgaagagc atgacggaga cgtagatccc gtgctcgttc gccgcctcga
     1441 cccgggcgcg gagccggtcg aagtacgcct ggtcgagctc cccgaggtcg aacttcggcc
     1501 cgccgtcgtg cgccgttccc gggccggtac gcggccatgg atggaacgcg gtcgtcgtct
     1561 ggcgcggatt cccgaggtcg tcgaagcaca tcgtccacct cgtgtgctcc acccgccaca
     1621 gccggacgaa gttgtggtcg tggtcctcga gccagtcgag gtggcggtcc cagtcgaagg
     1681 cggcgggggc gtcccagcag ccggacgtcc agtcgggcgc gccgaggttc caccagacgt
     1741 gcgagccggt caggtacacg gccttgcccg tcccgtcggt gaagtagcgc gggttcgact
     1801 cgagcacgcg gagcgggccc ttgaccgcgg cctccgccgc ggggacggcg aagagcgcgg
     1861 cgcagagcgc gcacgcgagg agggagaggc cctggcgcat cccggctgcg agcctagcgt
     1921 tcccggcggc gggcggtccg gaggccgcgc agcgacgcgc tcatctcggc ggcggcggcg
     1981 gggcggcgga gcaggcggaa ggcggccgcg cgcgccgcga ggccggccac gacgagcccc
     2041 gatgcgagcc gcgcccgggc cggcgagagg ttgcgggcga gcatggccgc ctccgcggcc
     2101 gcgacgcgtt ccgcgcgtgc ggggtcgcgc tcgtccccgc cgctcgagcc ggcgccgagg
     2161 tggacgaatt gggcgtcgtg gacgaagcgg acggtgcgcc ccagccggcg cgcgcgccag
     2221 cagaggtcga ggtcctccgc gtacatgtgc gacgactcgt cgaagccgcc gagcgcgtcg
     2281 aacgtctcgc gccggacgag cagcaccgcg ccgttggccg cctggatctc gcgcgactcc
     2341 gagtgatccc agtgcgtgct ccagcgcggc tggaagcggt tcgggacgag ccggctgagg
     2401 ccggaagcgc gcacggcggc gacggcgggc gtcgtgagcg ggacgacggt cggctggagc
     2461 gatccgtcct cgttcacgag ccggggcgcg acgacgccga cgcgcgggtc gtcgagcgcc
     2521 cgccgcatcc gctcgaccga gccgggccgg tcgacgaacg cgtcggagtt gacgaagagg
     2581 tacgcggccg ccggaagcgc ccgcgcgccg atgttgttcc cgcgcgcgaa gccgacgttc
     2641 tcctcgaggc gcacgagcac ggcgccgcgc agctcctctc cgaagcgggc gaacgagtcg
     2701 tcggccgagc cgttgtcgac gacgacgatc cgctcgagcg ggacaccgtc gtccgcgagc
     2761 gagcggacgc accggatcgt caggtccggc gtccgccagt tgaggacgac cgcagtgatc
     2821 aggggcgctc ccccgcgagc agccgggcgg gcgcgtgctc cgtcagctcc gcggcggcgg
     2881 cgtcgccgag ccggtcggcg accatgcgcg cggcgtcgcc taggtgcggg ctccgccgac
     2941 gcgtcccgtg ggagtcgctc gcgaggaggc cggcccgccc cgtgtccacg agccgccagc
     3001 cggccgcggc ggcgtctccg ccgaagcgcc cgatgaggct cggcgcgacg acctggagga
     3061 cgccgccgtc gcggaggatg tgatcggcga tccgcgcgct ccgctgcacc gcccggcagc
     3121 gctcgggatg gccgaagagg ggcaggagct cgatctcctc gagccgcgcg aagacggacg
     3181 ggaagaagct cgggggcgtg tcgcggagca cctcgacgag caggtagcgg ccgccgatcg
     3241 cgcggcggcg gagctcctcg agcggcgccg agaccgcgtg accggggctc acctccgcgg
     3301 cgagcgacgc ctcgagcccg agcgcctcga gctcgggccg gagctcgcgc agccgctcct
     3361 cggcgtcgtc gtggcgcgtc gggaactgcg acgcgtagtg cggcgtgcag aggacgcggt
     3421 cgatcccgtc cgcgaggagc gcaccggcca ggtcgagcgc atcctccctc gtcttcggcc
     3481 cgtcgtcgag gccggggagg aggtggcagt gcgtgtcgat cacgcgcgcg ccccggggcg
     3541 gagcgactcg aagacccggc agagcccgcg ggcggtctcc tcccacgagt agcgggcgac
     3601 cgtctccgcg ccgcgcaggc ggagatcggc gcgcagcgcc tcgtcgcggg cgatcctgcg
     3661 gatcccgtcg gcccacgcgt cctcgtcgcc gaccggcgcg agcagggcgc cgtcgccgga
     3721 gatctcccgc acggccggga tgtcgctcgc gagcaccggg cagccgtgcg ccatcccctc
     3781 gagcggcgtg aacccgaagc cctcgtagcg cgaggggagg aggagcgccg tcgccgcgcc
     3841 gtagagcgcg acgagggtcg agtcgggcgc tccctcgagg acggtcacgc gcgggccggc
     3901 cgcggcgagc gccgcgtcga gctcgggata gggctcgcgc gggcgtccga cgagcacgag
     3961 gccgtcgtcc accccggagc gcgcgtaggc ccgcacgagc gtcgccacgt ccttcttggc
     4021 cgtcaggtcg ccgacgtaga gcaggaacgg gcagggcggg agcccgtcgg cgggcgctgc
     4081 gccgtccgct tgaacgagcc ccgggccgga gcggacgacg gtcacccgct cgggcggcgt
     4141 cccgaagtgg cggcgcgcct cgtcgcgggc gaaatgggac accgcgacca cccgcgccgc
     4201 gcgtccgagc gcccaggggt agacgcgccg cttgtaggcg tggcgaacct tctcgaaccc
     4261 gtggacgatc gacgcgtcgt ggatcgtcgt cgtgaagcgc cggcgcggcc ggaggagcgg
     4321 gccggtcagg tcgaagaagt ggagcaggtc ggcgcggcgc gacgcggcgg cgagctgctc
     4381 tccggcgagc cgcgcggggc ccgagcgtgc gtcgtggaac gcggagcccg cgagcgcgac
     4441 ggcg
//
LOCUS       DASTFC010000171         5533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_19423,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000171 DASTFC010000000
VERSION     DASTFC010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19423"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1666
                     /locus_tag="VFO88_05130"
     CDS             215..1666
                     /locus_tag="VFO88_05130"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET9544938.1"
                     /translation="MRDLCRNAVDDALAGGASYADARTHERRSQTVRTKAREVDSVAD
                     SETEGIGVRVLVDGAWGFASSPDLSPAGAREAASRAVEFARASSSRAGARARRTELAP
                     TPPAHGEYSTPAGRDPVDVPLEDKIDLCLRAEEALERPGLALTSAFVRAQRERRAFAS
                     SEGADALQELCECGGGIEALALGDDGGVQTRSYPSAHGGSSAQAGWEYVEELGLEREA
                     PRVAEEALALLRAEPCPAGVTTIVLDSDQTGLQVHESVGHPTELDRIYGTEAAYAGTS
                     WVRAEDLGSLRYGSELMNITADATTPGGLGTFAWDDEGVPAAREPVVERGILAGFLTS
                     RETAARLGRGAGGSMRADGWNRMPLVRMTNLHLEPGEGSLEELLAGVDDGIFMATNKS
                     WSIDDKRLNFQFGTEIAWEIKRGTLGRILRDATYTGTTPVFWGSLDGVAGPQEWRLTG
                     LTNCGKGQPGQHAHVSHGASPARFRNVQVGVRS"
     gene            1663..3027
                     /locus_tag="VFO88_05135"
     CDS             1663..3027
                     /locus_tag="VFO88_05135"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET9544939.1"
                     /translation="MREAPEALELAARAVAAAEGDGVDAVAHRERSGLARFAASVVHQ
                     PTLVEDASVTIRVVRDGKVGAVSTNRVDDEGLREAARRAGEAAARGRPDPAFPGLAAP
                     APLPAVEGWDEETAALDPGAIAAHAWTAIEAAEGLGLYGYFTSGVTELAVASSTGVAV
                     SQALTDATVLALAADETRSGYAEATSWRVGELDPAAAGREAASIAARTEGAGELEPGT
                     YRALLSPYAFAELLLYFGYSSLGALALLEGRSFLAGRLGEKVFDERLSLADDGHDPHG
                     LPKAFDLEGVPKQRVQLVEAGALRDVVWDRRTAHRDGRESTGHGLDAASQAYGPLPFN
                     LSVAPGDATDEELAELVGDGIYVTRLHYVNIVDAREGLLTGMTRDGTFRVEGGRVTRP
                     LVNLRFTTSVPELLAHLPGIGAEARLVNASDFYDARYPFGSLVPPVATERFTIVAAGS
                     APGL"
     gene            complement(3038..3259)
                     /gene="secG"
                     /locus_tag="VFO88_05140"
     CDS             complement(3038..3259)
                     /gene="secG"
                     /locus_tag="VFO88_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET9544940.1"
                     /translation="MLAASAAVQVILSIILIVLVLMHSGRDTGFGGLGFTPASQGGQH
                     IVERNLTRLTVVVALIFSVNSVALFYLLD"
     gene            complement(3278..3667)
                     /locus_tag="VFO88_05145"
     CDS             complement(3278..3667)
                     /locus_tag="VFO88_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9544941.1"
                     /translation="MENAVADRRTVLIADDHDLFAESLGAFLGTEERIEVVGRASNGD
                     EAARMCAELRPDVVLMDISMPVLNGFDAAKLIRDCAPDTSVLFLTGSASSADVAQARA
                     AGGVGYVTKDKLATELVDAIVSAPVRA"
     gene            complement(3702..4628)
                     /locus_tag="VFO88_05150"
     CDS             complement(3702..4628)
                     /locus_tag="VFO88_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9544942.1"
                     /translation="MTHDHDHHHPGPDADTRLLLVALGVILAFTALEVLVGIVADSLA
                     LLADAGHMLTDASALAIALVAARAASRPPRGPWTFGFGRAEILAAQANGVALALVGIW
                     ITVEAVRRLLEPRDVDAALVLPVSLAGIAITGLVAALLARAERRSLNIRGAYLHILTD
                     VAAFAGTALAAVAILLTGWNRLDPAASLAVAALALWAAWTLVRDSGRIFLEAAPPSAP
                     PGEVGEAIVAHPGVVEAHDLHVWTVTSGFPALSAHVLVEPGADCHRIRLELERVLRER
                     FDVEHTTLQVDHVGVGSGLSIERNRQGNVGEH"
     gene            complement(4625..>5533)
                     /locus_tag="VFO88_05155"
     CDS             complement(4625..>5533)
                     /locus_tag="VFO88_05155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9544943.1"
                     /translation="VPAVVDRRSGSVAKQAGLLLRTLRGLRPRPDVVYAHYLVPAGAV
                     AALAATVARVPLVVTAHGRDVRNIGVVRGVRALTRLATRRAARVIAVSDYLRRDLVAR
                     LPELDGKVEVVSAGVDLERFAPRDGAEARARLRWEGEPPYFLCLGTLDERKNVVRLAD
                     AFAELGRGSLAFVGDGPAGAALESRPGVKLAGRVPHDAVPDWIAACDVLCQPSLVEPF
                     GIALLEAMAGERSVVATAVGGPPEFVTPEVGVLVDPASVEAIADGLRAAAKLPSPNPA
                     AREAVARHDVREEARRIAEILAAAAR"
BASE COUNT          743 a         2003 c         2135 g          652 t
ORIGIN      
        1 gtctgggccg atgccgtggt cggcatggcc atgaccgctc ccagcacgac gagcggcacg
       61 agaatgcgaa gcagtttggg catatgcgat cagacctccg agggaagctc ctggaaaagg
      121 ggctcgatcg taaaccattt atgggcgcga gtctaggggc cgttggggcc ttccaacggg
      181 tgttccaggc cgtcctctaa cctccctcct gcccatgcgc gacctgtgcc ggaacgccgt
      241 cgacgacgcc cttgcggggg gcgcgtcgta cgccgacgcg cgcacccacg agcgccgctc
      301 gcagaccgtc cgcacgaagg cccgggaggt cgattcggtg gccgactcgg agaccgaggg
      361 gatcggcgtc cgcgtcctcg tggacggtgc ctggggcttc gcctcgagcc ccgacctgtc
      421 cccggccggc gcgcgtgagg cggcgtcgcg cgcggtcgag ttcgcccgtg cgagctcctc
      481 gcgcgcgggc gcgcgcgcgc gaagaacaga gctcgcgccg acgcctcccg cgcacggcga
      541 gtacagcacg ccggccggcc gcgacccggt cgacgtgccg ctcgaggaca agatcgacct
      601 ctgcctgcgc gccgaggagg cgctcgagcg gcccggcctc gcgctgacct cggccttcgt
      661 gcgcgcccag cgcgagcggc gcgcgttcgc ctcctccgag ggggcggacg cgctccagga
      721 gctctgcgag tgcgggggcg ggatcgaggc gctcgcgctc ggcgacgacg gcggcgtgca
      781 gacccgctcg tacccctccg cgcacggcgg ctcgagcgcc caggcgggct gggagtacgt
      841 cgaggagctc ggcctcgagc gcgaggcccc gcgcgtcgcc gaggaggcgc tcgccctcct
      901 gcgggccgag ccctgtcccg ccggcgtgac gacgatcgtg ctcgactcgg accagacggg
      961 cctccaggtg cacgagtccg tcgggcaccc gaccgagctc gaccggatct acggcaccga
     1021 ggcggcctac gcgggcacga gctgggtgcg ggcagaggac ctcgggtcgc tccgctacgg
     1081 ctccgagctc atgaacatca ccgccgacgc gacgacgccg ggcggcctcg ggacgttcgc
     1141 ctgggacgac gagggcgtcc cggccgcacg cgagccggtg gtcgagcgcg ggatcctcgc
     1201 cggcttcctc acctcgcgcg agacggccgc gcggctcggc cgcggcgcgg gcggctcgat
     1261 gcgcgcggac gggtggaacc ggatgccgct cgtccggatg acgaacctcc acctcgagcc
     1321 cggcgagggc tcgctcgagg agctgctcgc cggcgtggac gacggcatct tcatggccac
     1381 gaacaagagc tggtcgatcg acgacaagcg gctcaacttc cagttcggca ccgagatcgc
     1441 ctgggagatc aagcgcggga cgctcggccg gatcctccgc gacgcgacgt acaccgggac
     1501 gacgccggtc ttctggggct cgctcgacgg cgtcgcggga ccgcaggagt ggcggctgac
     1561 cgggctcacg aactgcggca agggccagcc gggtcagcac gcgcacgtct cgcacggcgc
     1621 gtcgcccgcg cggttccgga acgtccaggt cggcgtgcgc tcgtgaggga ggcgcccgag
     1681 gccctcgagc tcgccgcgcg cgcggtcgcc gcagcggagg gagacggggt ggacgcggtc
     1741 gcgcatcgcg agcgatccgg gctcgcgcgc ttcgccgcct ccgtcgtcca ccagccgacg
     1801 ctcgtcgagg acgcgtccgt cacgatccgg gtcgtccggg acgggaaggt cggcgcggtc
     1861 tcgacgaacc gcgtcgacga cgagggcctc cgggaggccg cgcggcgcgc cggcgaggcg
     1921 gccgcacgcg ggcggcccga cccggcgttc cccgggctcg cggcgccggc cccgctcccc
     1981 gccgtcgagg gctgggacga ggagacggcc gcgctcgacc ccggcgccat cgcggcgcac
     2041 gcgtggacgg cgatcgaggc ggcggagggg ctcgggctct acggctactt cacgagcggc
     2101 gtcaccgagc tcgcggtcgc gtcgtccacc ggcgtcgcgg tctcacaggc tctcacggac
     2161 gcgaccgtcc tcgcgctcgc ggccgacgag acgcgctccg gctacgcgga ggcgacctcc
     2221 tggcgcgtcg gcgagctcga cccggccgcc gccggccgcg aggcggcctc gatcgccgct
     2281 cggaccgagg gcgcgggcga gctggagccg ggcacgtacc gcgcccttct ctcgccgtac
     2341 gccttcgcgg agctcctcct ctacttcggg tacagctcgc tcggcgcgct tgcgctgctc
     2401 gagggcagga gcttcctcgc cggccggctc ggggagaagg tcttcgacga gcggctctcg
     2461 ctcgcagacg acggccacga cccgcacggg ctgcccaagg cgttcgacct cgagggggtc
     2521 ccgaagcagc gcgtccagct cgtcgaggcg ggcgccctcc gcgacgtcgt ctgggaccgg
     2581 cgcacggccc accgcgacgg tcgcgagtcg acgggccacg ggctcgacgc ggcctcgcag
     2641 gcgtacgggc cgcttccctt caacctgagc gtcgcgccgg gcgacgcgac cgacgaggag
     2701 ctcgccgagc tcgtgggcga cgggatctac gtcacgcgcc tccactacgt gaacatcgtc
     2761 gacgcacgcg aggggctcct cacggggatg acgcgcgacg gcacgttccg cgtcgagggc
     2821 ggccgcgtga cccggccgct cgtgaacctc cgcttcacga cctcggtgcc cgagctcctc
     2881 gcgcacctcc ccgggatcgg cgcggaggcg cggctcgtga acgcgagcga cttctacgac
     2941 gcgcgctatc ccttcggctc gctcgtcccg cccgtcgcga ccgagcggtt cacgatcgtc
     3001 gccgccggct cggcgccggg gctttagtag gcgggcctca gtcgaggagg tagaagagcg
     3061 cgaccgagtt cacgctgaag atgagcgcga cgacgacggt caggcgggtg aggttccgct
     3121 cgacgatgtg ctggccgccc tgggaggccg gcgtgaagcc gagcccgccg aagcccgtgt
     3181 cccggcccga gtgcatgagc acgagcacga tgaggatgat ggacaggatg acctgcacgg
     3241 ctgcggaagc ggcgagcacg gggccaggat accggggcta cgcccgcacc ggcgccgaca
     3301 cgatcgcgtc gacgagctcc gtggcgagct tgtccttcgt cacgtagccg accccgcccg
     3361 cggcgcgcgc ctgggcgacg tcggccgacg aggcggagcc ggtgaggaag agcaccgagg
     3421 tgtcgggcgc gcagtcgcgg atgagcttcg ccgcgtcgaa gccgttcagg accggcatgc
     3481 tgatgtccat gaggacgacg tccgggcgca gctccgcgca catgcgggcc gcctcgtcgc
     3541 cgttcgacgc ccggccgacg acctcgatcc gctcctccgt gccgaggaag gcgccgaggc
     3601 tctcggcgaa gaggtcgtga tcgtcggcga tcaggacggt gcggcggtcg gcgactgcgt
     3661 tttccacggg ctcccggtta tgaaggcaaa gcgaaggaat gtcaatgttc gccaacattt
     3721 ccctgacggt ttcgctcgat cgagagcccc gatccgacgc cgacatggtc gacctggagc
     3781 gtcgtgtgct cgacgtcgaa gcgctcgcgc agcacccgct cgagctcgag ccggatgcgg
     3841 tggcagtcgg ccccgggctc cacgaggacg tgcgcggaga gcgccgggaa gccgctcgtc
     3901 accgtccaga cgtggaggtc gtgcgcctcg acgacgcccg ggtgggcgac gatcgcctcg
     3961 cccacctcgc cgggcggcgc cgagggcggc gcggcctcga ggaagatccg gccggagtcg
     4021 cggacgagcg tccacgcggc ccagagcgcg agcgcggcga ccgcgagcga cgcggccggg
     4081 tcgaggcggt tccagccggt gaggaggatc gcgacggcgg cgagcgccgt gccggcgaac
     4141 gcggcgacgt ccgtgaggat gtggagatac gcgccgcgaa tgttgaggct ccgccgctcg
     4201 gcccgcgcga ggagggcggc gacgagcccg gtgatcgcga tcccggcgag cgagacgggc
     4261 aggacgagcg cggcgtccac gtcgcgcggc tcgagcagcc ggcggacggc ctcgaccgtg
     4321 atccagatcc cgaccagcgc gagcgcgacc ccgttcgcct gcgcggcgag gatctcggcg
     4381 cgcccgaagc cgaacgtcca cgggccgcgc gggggccgcg aggcggcgcg ggcggcgacg
     4441 agcgcgatgg cgagcgcgct cgcgtccgtc agcatgtggc cggcgtcggc gaggagggcg
     4501 agcgagtcgg ccacgatccc gacgaggacc tcgagcgccg tgaaggcgag gatgacgccg
     4561 agcgcgacga ggagcagccg ggtgtcggcg tccgggcccg ggtggtggtg gtcgtggtcg
     4621 tgcgtcatcg ggccgccgcg gcgaggatct cggcgatccg ccgggcctcc tcccggacgt
     4681 cgtggcgcgc gaccgcctcg cgcgcggccg ggttcgggga cgggagcttc gccgcggcgc
     4741 gcaggccgtc ggcgatcgcc tcgacgctcg cggggtcgac gaggacgccg acctccggcg
     4801 tgacgaactc cggcgggccg ccgaccgcgg tggcgacgac cgaccgctcg cccgccatgg
     4861 cctcgagcag ggcgatcccg aacggctcga cgaggctcgg ctggcagagg acgtcgcagg
     4921 cggcgatcca gtcgggcacg gcgtcgtgcg ggacgcgtcc ggcgagcttc acgcccggcc
     4981 ggctctcgag cgccgcgccg gccgggccgt cgcccacgaa cgcgaggctg ccgcgcccga
     5041 gctccgcgaa cgcgtcggcg aggcggacga cgttcttccg ctcgtcgagc gtgccgaggc
     5101 agaggaagta cggcggctcc ccctcccagc ggaggcgggc ccgcgcctcg gcgccgtcgc
     5161 gcggcgcgaa gcgctcgagg tcgacgccgg cggagaccac ctccaccttc ccgtcgagct
     5221 cgggcagccg cgcgacgagg tcccgccgga ggtagtccga gacggcgatc acgcgcgcgg
     5281 cgcggcgcgt ggcgagccgc gtgagcgcgc ggaccccgcg caccacgccg atgttgcgga
     5341 cgtcgcggcc gtgcgcggtc acgacgagcg ggacacgggc cacggtcgcg gcgagcgcgg
     5401 cgaccgcccc ggcggggacg aggtagtgcg cgtacacgac gtccggccgc ggccggagcc
     5461 cgcgcagcgt gcgcaggagc agcccggcct gcttcgcgac ggagcccgag cggcggtcga
     5521 cgaccgcggg gac
//
LOCUS       DASTFC010000172         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_250414,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000172 DASTFC010000000
VERSION     DASTFC010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250414"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFO88_05160"
     CDS             <1..499
                     /locus_tag="VFO88_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9544944.1"
                     /translation="LEKPVIAGVNGAAAGAGMSLACACDLRIASDSASFVPAFIGIGL
                     VPDTGGTWFVTQLLGYARAFEWLTSGRKLSAADAHAWGLVNEVVPADELSNRVAEVAG
                     RYAAAPTRGIGMTKRLLDRASRSRLEEQLEWEAQLQAAAAASEDFREGVAAFLEKREP
                     RFTGR"
     gene            complement(486..944)
                     /locus_tag="VFO88_05165"
     CDS             complement(486..944)
                     /locus_tag="VFO88_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544945.1"
                     /translation="MRVALALLACSAAAFAGCSGGGGEPEVRLTAEQWIAAADRICAE
                     YEGRLDELREPDDLDGLATMAAEAEPIAEEGLEKLRRLGPPEELEEQADEWLARTAEN
                     VRAIGELREAAEDGDETRVQELASAGLDNEAVADRLARDLGLDECAASDR"
     gene            complement(954..1244)
                     /locus_tag="VFO88_05170"
     CDS             complement(954..1244)
                     /locus_tag="VFO88_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9544946.1"
                     /translation="MSQERVTKLSPAAFERIRERIAHAENGWFFAQIADRVADRRREL
                     GLSQAQLAELTGTTQSAVARLESGGRPPRIDTLLRIADALDCELAVELRPRG"
     gene            1299..>3005
                     /locus_tag="VFO88_05175"
     CDS             1299..>3005
                     /locus_tag="VFO88_05175"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET9544947.1"
                     /translation="MSASVEDRLAVLEGHTRALYDRLERVEAELRGRPAAPPRAAAPV
                     PPPPAPPPRPEVRPSRPPTRPVAPPRPRRELDLEELLGGRLLALVGGLAVILGLVFLV
                     ALAVERGWLDERARTALAFFGSTALVLVGAWLHERRGRTQASLAVVGTGLAGLFLSLT
                     AATVLYDLVPVGLSLLAAFAFGALGAALAIRWDAQPIGGLGILGALSAPILVGATQDA
                     QSLLFLAAAHAAAVAVLVWRRWEWLRLAAVAVVLLQLAGWVLEDEPGLTLGLTVLGLF
                     GVLNLAAALGFELARRADRGAASTFALVVGNAAAVGLLGVYVAWLPWDDAGSVVAAGW
                     WLAGLAAAHVLAGLALLRLQPRNRSVALRLFGVGLVAANVAFVVLVDGVAIPIGWAAA
                     AAALALPVRELTRRAAVVYAVVGGQLVLAGLHILTIDAPLDALEPDRTAVWPILALAA
                     SAFVVARLTPREEGQWRAATDATALLAVAYATAVLLDGVVLTGAWAVEAVLLSELGRR
                     LSHRVAAVGGLGFLLLAALHALSFEAQPVALVDGADPFWHAAVALGAIACGAAFAAWR
                     GLD"
BASE COUNT          281 a         1178 c         1089 g          457 t
ORIGIN      
        1 gctcgagaag ccggtcatcg cgggcgtgaa cggcgcggcc gccggggccg ggatgtcgct
       61 cgcgtgcgcc tgcgacctcc ggatcgcgtc cgactcggcg agcttcgtcc ccgcgttcat
      121 cgggatcggg ctcgtccccg acaccggcgg cacgtggttc gtgacgcagc tcctcggcta
      181 cgcgcgggcg ttcgaatggc tcacctccgg gcgcaagctc tcggccgccg atgcgcacgc
      241 atggggcctc gtgaacgagg tcgtgccggc ggacgagctc tcgaaccgcg tcgccgaggt
      301 cgccgggcgc tacgcggccg cgccgacccg cgggatcggc atgacgaagc gcctcctcga
      361 ccgcgcctcg cgctcccggc tcgaggagca gctcgagtgg gaggcgcagc tccaggcggc
      421 cgccgcggcg agcgaggact tccgcgaggg agtggccgcg ttcctcgaga agcgcgagcc
      481 ccgcttcacc ggtcgctagc ggcgcactcg tcgagcccga ggtcccgcgc gagccggtcg
      541 gcgaccgcct cgttgtccag gcccgcgctc gcgagctcct gcacccgcgt ctcgtcgccg
      601 tcctcggcgg cctcgcggag ctcgccgatc gcgcgcacgt tctcggcggt ccgcgcgagc
      661 cactcgtcgg cctgttcctc gagctcctcg ggcggcccga gccggcggag cttctcgaga
      721 ccctcctcgg cgatcggctc cgcctccgcg gccatcgtcg cgagcccgtc gaggtcgtcc
      781 ggctcgcgga gctcatcgag gcgtccctcg tactcggcgc agatccggtc ggccgccgcg
      841 atccattgct ccgccgtcag gcgcacctcg ggctctccgc cgccgccgct gcagccggcg
      901 aacgcggccg cggagcatgc caggagggcg agcgcgaccc gcacgcgccg accctaaccg
      961 cgcggacgga gctcgaccgc gagctcgcag tcgagcgcgt cggcgatccg cagcagcgtg
     1021 tcgatccgcg gcggccgacc gccgctctcg agccgcgcca cggcggactg cgtcgtgccc
     1081 gtcagctcgg cgagctgggc ctgcgacagg ccgagctcgc gccggcggtc ggccacccgg
     1141 tcggcgatct gtgcgaagaa ccagccgttc tccgcgtggg cgattcgctc gcggattcgc
     1201 tcgaaggccg cgggactgag cttggtaacg cgctcttgac tcatatcgca aacgtggtat
     1261 atagcaacta agctatccga tcgtagcagg aggcagtcat gagcgcgagc gtcgaagatc
     1321 gcctggcagt cctcgagggt catacccggg ccctgtacga ccggctcgag cgggtcgagg
     1381 ccgagctccg cggccggccg gcggctccgc cgcgtgcagc cgcgccggtt cctccgcccc
     1441 cggctccgcc gccccggccg gaggtgcggc cgtcgcggcc gccgacgcgg cccgtcgcgc
     1501 cgccgcggcc gcggcgcgag ctcgacctcg aggagctgct cggcggccgg ttgctcgcgc
     1561 tcgtcggcgg acttgcggtc atcctcgggc tcgtcttcct cgtcgcgctc gccgtcgagc
     1621 gcggctggct cgacgagcgg gcccgcacgg cgctcgcgtt cttcggctcg accgcgctcg
     1681 tcctcgtcgg agcgtggctg cacgagcgcc gcggccgcac gcaggcgtcg ctcgccgtcg
     1741 tcggcaccgg actggcgggc ctcttcctct cgctgaccgc cgcgaccgtg ctctacgacc
     1801 tcgtccccgt cggtctctcc ctcctggccg cgttcgcgtt cggcgccctg ggagccgcgc
     1861 tcgcgatccg ctgggacgcg cagccgatcg gcggcctcgg gatcctcggc gcgctctccg
     1921 ccccgatcct cgtcggcgcg acgcaggacg cccagtccct cctcttcctc gccgccgccc
     1981 atgccgcggc ggtggcggtg ctcgtctggc ggcgctggga gtggctacgc ctcgccgcgg
     2041 tcgcggtcgt gctcctccag ctcgccggct gggtgctcga ggacgagcct gggctcacgc
     2101 tcgggctcac cgtgctcggg ctcttcggcg tcctgaacct cgcggcggcg ctcgggttcg
     2161 agctcgcgcg ccgtgccgac cggggcgcgg catcgacctt cgccctcgtc gtcgggaacg
     2221 cggccgccgt cgggctgctc ggcgtctacg tggcctggct gccgtgggac gacgccggct
     2281 cggtcgtcgc ggcgggctgg tggctggccg gtctcgcggc cgcgcacgtc ctcgcggggc
     2341 tcgcgctcct gcgcctgcag ccgcggaacc gctcggtcgc gctccggctc ttcggcgtcg
     2401 gcctcgtcgc cgcgaacgtt gccttcgtcg tgctcgtgga cggcgtggcg atcccgatcg
     2461 gctgggccgc agccgccgcg gcgctcgcgc ttccggtgcg ggagctcacc cgccgcgccg
     2521 ccgtcgtcta cgcggtcgtc ggaggccagc tcgtcctcgc cggcctgcac atcctgacga
     2581 tcgacgcgcc gctcgatgcg ctcgagccgg acaggaccgc ggtctggccg atcctcgcgc
     2641 tcgcggcaag cgcgttcgtc gtcgcccggc tgaccccgcg ggaggagggt cagtggcgcg
     2701 cggcgaccga tgcgacggcg cttctcgcgg tcgcctacgc gacggccgtc ctcctcgacg
     2761 gcgttgtgct cacgggcgcc tgggcggtcg aagccgtgct cctctcggag ctcggccgcc
     2821 gcctctcgca ccgcgtcgcg gccgtcggcg ggctcggctt ccttctcctt gcggctctcc
     2881 acgcgctctc gttcgaggcg cagccggtgg cgctcgtgga cggcgccgac ccgttctggc
     2941 acgcggccgt cgcgctcggc gcgatcgcgt gcggcgcggc cttcgccgcg tggcgcgggc
     3001 tcgac
//
LOCUS       DASTFC010000173         7086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_233831,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000173 DASTFC010000000
VERSION     DASTFC010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233831"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFO88_05180"
     CDS             <1..657
                     /locus_tag="VFO88_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9544948.1"
                     /translation="LSYDVASSLEGSDIEVPTALGFDVRLTDRIVLLVRADAQGGKLR
                     VLRAEAQPFATNLSVPTMFGQIEITRGWVAADVRLRGRTFRVVNTHLEPFSTPVQVAQ
                     AQELLAGPLAPGRPALALGDFNSGPGRPTPVYGMLLGAGFADAAANAGLTCCHADDLR
                     NPTPAFTQRIDLVLARGGFTPLRAEVVGDEQAVRDALGLWPSDHGGPVATLRLPHPRR
                     "
     gene            720..974
                     /locus_tag="VFO88_05185"
     CDS             720..974
                     /locus_tag="VFO88_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544949.1"
                     /translation="MPRWPPLHPEVAAALEALDAIGMPYAELWRLLRPVAQRAGCRPP
                     SYWLVRRRAIELRRRKLERARIAAELYGDMVTGRLPYRFR"
     gene            complement(995..1873)
                     /gene="sucD"
                     /locus_tag="VFO88_05190"
     CDS             complement(995..1873)
                     /gene="sucD"
                     /locus_tag="VFO88_05190"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET9544950.1"
                     /translation="MAIVVDRDTRLVVQGITGREGSFHATRNKAYGTQVVAGVTPGKG
                     GLDVEGIPVFDTVTGAVAETDANTAMVFVPARFAADAIYEAVDAGIRTVITITEGIPA
                     HDMLRVYNYVRPRGITLIGPNCPGALSPGKANVGIIPAEIFTEGRVGLVSRSGTLTYQ
                     IGHELTRLGIGQSTIVGIGGDPVVGSSFIDILEKFEADPETELVVMVGEIGGDEEEKA
                     AAFIGERMSKPVVAYIAGFTAPPGKTMGHAGAIISGSAGTAQAKKEALEARGVRVGTN
                     PTEVAELVADAVGARA"
     gene            complement(1874..3025)
                     /gene="sucC"
                     /locus_tag="VFO88_05195"
     CDS             complement(1874..3025)
                     /gene="sucC"
                     /locus_tag="VFO88_05195"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET9544951.1"
                     /translation="MDLYEYQGKELFRRHGIPVSAGRLATTPEEARSAADSLGSEVVV
                     KAQVLTGGRGKAGGIKLADSPQEAEHHAREILGMDIRGHVVKKVWIEEASEIAKEYYL
                     SLTFDRGAKKPLFMFTTQGGVDIEEVAEKTPEALAKLHVDPLAGLQPYHARRLLFAAG
                     IDEPGEQKQIAAIVEKLYAAFVAEDAMLCEINPLIVEPHGRVKALDSKFTVDDNALYR
                     HPDVAEMRDLDAYPAEERAAREKGVTYVKLDGEVGILGNGAGLVMSTLDVIAQAGGRP
                     ANFCDLGGGGDAQGVVDALEVITPDPQVRSILFNVFGGITRCDEVAQGILDALGRMEI
                     AQPIVVRLDGTNADEGRQILADASPPNLHVEETMLSAARRAVELAEEAA"
     gene            3133..3321
                     /locus_tag="VFO88_05200"
     CDS             3133..3321
                     /locus_tag="VFO88_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544952.1"
                     /translation="MSTLRDDEITGGTDEVLDPTAQSDVDADDADDADSSDAGDQGDA
                     TDDSDAADPDVGPADSAS"
     gene            3278..4504
                     /locus_tag="VFO88_05205"
     CDS             3278..4504
                     /locus_tag="VFO88_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9544953.1"
                     /translation="MPQIRTSAPRTPRASSTGALARCVEPIPAEEFLAEYWEERPLVQ
                     LRDGDASFDDLLSEADVERLVTSGELRHPAFRLVKAGEKLRLGDYSTDIPWRPSAFRG
                     VADAERVAAAFAEGATIVVQGLHHWWRPLSVFCRALEADLGEPAQANAYYTPRGSQGL
                     PVHHDTHDVFVLQVAGEKRWLVYGPAWELPLKDQRYSEGEHGPAGEVVKDVTLRAGDT
                     LYLPRGWLHEAVTSESDSLHLTIGVNVYTWSDALRGALRELSSEVDVRRSVPADGSGG
                     RELLDLLAERLEPDEVVRRKARRLVHGRRPILDGQLRQLRALESLTLDSEIVRRPEVL
                     YFLEDGVDGGRVRLSFAGKSLTFPSRARDEVEALAEADEPVTPRDLPGSLDEAGRLVL
                     VRRLVREGYLRIVGVE"
     gene            complement(4514..5452)
                     /locus_tag="VFO88_05210"
     CDS             complement(4514..5452)
                     /locus_tag="VFO88_05210"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HET9544954.1"
                     /translation="MARDADPDARSRPFCAEASAARHEPIGGTASRIDHWVLVEYRRL
                     WSRDLLAGSGLSDEVKGHLREQLAALSRPRLLFVRRPDRREHDRIAVFFGRSGERGGR
                     FHALEIGDYEELRGIDMAGVLDGSAPAPPPLERPLLVVCTHGKRDRCCARYGRPLYDA
                     LRAREEVRECVWQSTHVGGDRFAGNLVCLPEGVYFGRVTPAGAGAVVDEYRAGRIELE
                     HYRGRAAYGFAAQAAEIRIREETGLRGLDDLALAGLEPRGEDAWEVRFRERATGETHA
                     VEVACERDDAAAFLTCDAAAPAHARRFVAGASRAPA"
     gene            5549..5818
                     /locus_tag="VFO88_05215"
     CDS             5549..5818
                     /locus_tag="VFO88_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544955.1"
                     /translation="MLRKLVVLTVAVVAVLAIASPASAARPATAPDVKCLQTGLGVLK
                     ANGGVAYFAKNGVPLSLIGGEGTAPLSTVLKLHLFQPSLFPWCAR"
     gene            complement(5866..>7086)
                     /locus_tag="VFO88_05220"
     CDS             complement(5866..>7086)
                     /locus_tag="VFO88_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9544956.1"
                     /translation="GEAYFSDPTVYVEKLILDPRHVEVQVLADAHGNAVHLGERDCTI
                     QRRHQKLVEETPSPAVSPELRARIGEIALDAARAVGYRGAGTIEGLLDPEGAYYFLEM
                     NTRIQVEHTVTELVTGIDLVREQILVAAGEPLAYGQDDVTFRGHAIECRINAEDAANG
                     FLPTPGTITAYREPAGPGIRVDSGVEAGSEISATYDPMVAKLCVWDLDRERARRRMLR
                     ALEEYVVEGVTTLIGFHRALLEHPCFAAGSTCHGIVESEELAQRSQELSHTATTVARA
                     PDGALRGRAVAVELDGRRYDVTVLEPEPPHAELARRRRERSPSHGGAHAAAKEAVVSP
                     MQGTVLAVEVAEGDEVEAGRVVCVVEAMKMENEIRAHRDGIVTNLSVSAGDAVSTGQV
                     LCVVTSDGAAPGPE"
BASE COUNT          911 a         2647 c         2515 g         1013 t
ORIGIN      
        1 ctctcgtacg acgtggcgtc gtcgctcgag ggctcggaca tcgaggtgcc gaccgcgctc
       61 gggttcgacg tccggctgac cgaccggatc gtcctgctcg ttcgcgccga cgcgcagggc
      121 gggaagctgc gcgtcctgcg cgcggaggcg cagcccttcg cgacgaacct ctcggtcccc
      181 acgatgttcg ggcagatcga gatcacgcgc ggctgggtcg ccgcggacgt gcggctgcgg
      241 gggcggacgt tccgcgtcgt gaacacgcac ctcgagccgt tctcgacacc ggtccaggtc
      301 gcgcaggcgc aggagctcct cgcggggccg ctcgcgcccg ggcgcccggc ccttgcgctc
      361 ggcgacttca actcggggcc gggacgtccg acgccggtct acgggatgct cctgggcgcc
      421 ggtttcgccg atgcggcggc gaacgccggc ctcacctgct gccacgcgga cgacctgcgc
      481 aacccgacgc ccgcgttcac gcagcgcatc gacctcgtgc tcgcgcgcgg cggcttcacg
      541 ccgctgcgcg cggaggtcgt cggggacgag caggccgtcc gcgacgcgct cggcctgtgg
      601 ccgtccgacc acggcggccc ggtcgccacg ctgcgcctcc cccatccgcg ccgctagcgg
      661 cacacctttc tcacatcgtg ttgcatcacc cgcgcgcttt cgcgcggcac gctcgcaccg
      721 tgccccgctg gccgccgctc catccggaag tcgccgccgc cctcgaggcg ctcgacgcga
      781 tcgggatgcc gtacgccgag ctctggcggc tcctccgccc ggtggcacag cgggccggct
      841 gccgaccgcc gagctactgg ctcgtgcggc gccgggcgat cgagctccgt cgccgcaagc
      901 tcgagcgggc gcgaatcgcg gccgagctct acggagacat ggtcaccggg cggttgccgt
      961 accggttccg gtgatgcaac acaagagctc ggggctatgc gcgtgccccg accgcgtccg
     1021 cgacgagctc ggcgacctcg gtcgggttcg tgccgacccg gacgccgcgg gcctcgaggg
     1081 cttccttctt cgcctgcgcg gtgcctgcgg agccggagat gatcgcgccg gcgtggccca
     1141 tcgtcttccc gggcggcgcc gtgaagccgg cgatgtaggc gacgacgggc ttcgacatgc
     1201 gctcgccgat gaacgcggcc gccttctcct cctcgtcacc gccgatctcc ccgaccatca
     1261 cgacgagctc ggtctcggga tcggcctcga acttctccag gatgtcgatg aacgacgagc
     1321 cgacgaccgg gtcgccgccg atcccgacga tcgtcgactg gccgatcccg agccgcgtca
     1381 gctcgtggcc gatctggtac gtgagcgtgc cggaccgcga gacgagcccg acgcggccct
     1441 cggtgaagat ctcggccggg atgatcccga cgttcgcctt tcccggcgag agcgcgcccg
     1501 ggcagttcgg cccgatgagc gtgatcccgc gcgggcgcac gtagttgtag acgcgcagca
     1561 tgtcgtgggc cgggatcccc tccgtgatcg tgatcaccgt gcggatcccg gcgtcgaccg
     1621 cctcgtagat cgcgtccgcg gcgaagcgcg ccgggacgaa gaccatcgcg gtgttcgcgt
     1681 ccgtctccgc gaccgcgccc gtgaccgtgt cgaagacggg gatgccctcg acgtccaggc
     1741 cgcccttccc cggcgtcacg ccggcgacga cctgcgtgcc gtacgccttg ttccgcgtcg
     1801 cgtggaagga gccctcgcgc ccggtgatcc cctggacgac gagccgcgtg tccctgtcga
     1861 cgacgatcgc cacctaggcc gcctcctccg cgagctcgac ggcgcggcgc gccgcggaga
     1921 gcatcgtctc ctccacgtga aggttcggcg gcgaggcgtc ggcgaggatc tgccggccct
     1981 cgtccgcgtt cgtcccgtcg aggcggacga cgatcggctg ggcgatctcc atccggccga
     2041 gcgcatcgag gattccctgc gccacctcgt cgcagcgcgt gatcccgccg aagacgttga
     2101 agaggatcga gcgcacctgc ggatcgggcg tgatcacctc gagcgcgtcc acgacgccct
     2161 gcgcgtcgcc gccgccgccg aggtcgcaga agttcgccgg ccgcccgccc gcctgggcga
     2221 tcacgtcgag cgtggacatg acgagcccgg ccccgttccc gaggatcccg acctcgccgt
     2281 cgagcttcac gtacgtgacg cccttctccc gcgcggcgcg ctcctcggcg ggatacgcgt
     2341 cgaggtcgcg catctccgcc acgtcgggat ggcggtagag cgcgttgtcg tccacggtga
     2401 acttggagtc gagcgccttc acgcggccgt gcggctcgac gatcagcggg ttgatctcgc
     2461 agagcatcgc gtcctcggcg acgaacgcgg cgtagagctt ctcgacgatc gcggcgatct
     2521 gcttctgctc gcccggctcg tcgatccccg cggcgaagag caggcgacgc gcgtggtacg
     2581 gctggagccc ggcgagcggg tcgacgtgga gcttcgcgag cgcctccggc gtcttctcgg
     2641 cgacctcctc gatgtcgacg ccgccctgcg tcgtgaacat gaagagcggc ttcttcgcgc
     2701 cccggtcgaa cgtgagcgag aggtagtact ccttcgcgat ctccgaggcc tcctcgatcc
     2761 agaccttctt cacgacgtgg ccgcggatgt ccatgccgag gatctcgcgc gcgtggtgct
     2821 ccgcctcctg gggcgagtcc gcgagcttga tcccgccggc cttgccgcgc ccgcccgtga
     2881 ggacctgggc cttcacgacg acctccgagc cgagcgagtc ggcggccgag cgggcctcct
     2941 ccggcgtcgt cgcgagccgc ccggcagaga ccgggatgcc gtggcgcctg aagagctcct
     3001 tgccctggta ctcgtagagg tccacggcgg gcgagtctac gggatgcggg ttccgcgggc
     3061 cggcgttccg ggcaggaacg aggcgttgta ggctcgcgca ggcgagcccc gaccccgtca
     3121 gaggaggcac gaatgtccac gctgcgagac gacgagatca ccggcggtac cgacgaggtc
     3181 ctcgacccga ccgcccagtc ggacgtcgac gcggacgacg cggacgacgc ggactcgagc
     3241 gacgccggcg accagggcga cgcgaccgac gacagcgatg ccgcagatcc ggacgtcggc
     3301 cccgcggact ccgcgagcta gctcgaccgg cgcgctcgcg cgctgcgtcg agccgatccc
     3361 ggccgaggag ttcctcgccg agtactggga ggagcgcccg ctcgtccagc tccgcgacgg
     3421 cgacgcctcg ttcgacgacc tcctctcgga ggcggacgtc gagcggctcg tcacctccgg
     3481 ggagctccgc cacccggcct tccggctcgt gaaggcgggc gagaaactgc ggctcggcga
     3541 ctactcgacg gacatcccct ggcgcccgag cgccttccgc ggcgtcgcgg acgccgagcg
     3601 cgtggcggcc gcgttcgcgg agggcgcgac gatcgtcgtc caggggctcc accactggtg
     3661 gcggccgctc tcggtcttct gcagggcgct cgaggccgac ctcggcgagc cggcgcaggc
     3721 caacgcgtac tacacgcccc gaggctcgca gggcctgccg gtgcaccacg acacgcacga
     3781 cgtcttcgtc ctccaggtcg ccggcgagaa gcgctggctc gtgtacgggc cggcctggga
     3841 gcttccgctc aaggaccagc gctactcgga gggcgagcac ggcccggcag gggaagtcgt
     3901 gaaggacgtg acgctccgcg cgggcgacac gctctacctc ccgcgcggct ggctccacga
     3961 ggcggtcacg tcggagagcg actcgctcca cctgacgatc ggcgtgaacg tctacacgtg
     4021 gtcggacgcg ctgcgcgggg cgctccgtga gctctcgtcc gaggtggacg tacgccgctc
     4081 tgttcccgcc gacggcagcg gcggccgcga gctcctcgac ctgctcgccg agcggctcga
     4141 gccggacgag gtcgtacggc ggaaggcgcg caggctcgtg cacgggcgac ggccgatcct
     4201 cgacggccag ctccgccagc tccgcgcgct cgagagcctg acgctcgaca gcgagatcgt
     4261 ccgccgtccc gaggtcctct acttcctcga ggacggcgtg gacggcggcc gcgtgcggct
     4321 ctcgttcgcc gggaagtcgc tcaccttccc ctcgcgtgcg cgcgacgagg tggaggcgct
     4381 cgccgaggcg gacgagccgg tcacgcctcg cgacctgccc ggctcgctcg acgaggcagg
     4441 acggctcgtg ctcgtccgcc ggctcgtgcg ggagggctac ctccgcatcg tgggcgtcga
     4501 gtagcccgcc gggctaggcc ggcgcgcggg aggcgccggc gacgaagcgc cgcgcgtggg
     4561 ccggcgccgc ggcgtcgcac gtcagaaacg cagccgcgtc gtcacgctcg caggcgacct
     4621 cgaccgcgtg cgtctcgccc gtcgcgcgtt cgcggaagcg cacctcccag gcgtcctcgc
     4681 cgcgcggctc cagcccggcg agcgcgaggt cgtcgaggcc gcgaagcccg gtctcctcgc
     4741 ggatccggat ctcggccgcc tgggccgcga agccgtacgc ggcgcggccc cggtagtgct
     4801 cgagctcgat ccgccccgcc cggtactcgt ccacgaccgc gcccgcgccg gccggcgtca
     4861 cacggccgaa gtagacgccc tcgggcaggc acacgaggtt gccggcgaag cggtcgccgc
     4921 cgacatgcgt cgactgccag acgcattcgc gaacctcctc gcgcgcgcga agcgcgtcgt
     4981 agagcggccg gccgtagcgg gcacagcagc ggtcgcgctt cccgtgcgtg cacaccacga
     5041 ggagcgggcg ctcgagcggc ggcggggcgg gcgccgagcc gtccaggacg ccggccatgt
     5101 cgattccgcg gagctcctcg tagtcgccga tctcgagcgc gtggaagcgt ccgccgcgct
     5161 cgcccgagcg gccgaagaag acggcgatcc ggtcgtgctc ccgccggtcc ggccgccgga
     5221 cgaagaggag ccgcggccgg gagagcgcgg cgagctgctc gcgcaggtgg cccttcacct
     5281 cgtcggagag gccgctcccc gcgaggagat cgcggctcca gagcctgcgg tactcgacga
     5341 gcacccagtg gtcgatgcgg ctcgccgttc cgccgatcgg ctcgtggcgg gcggccgagg
     5401 cctcggcgca gaacggccgg ctccgagcgt cggggtccgc gtcgcgcgcc acggccgtca
     5461 cggtagccga gcccgatatt ggacagttac ttgacaatca ttctacgaat cctctacagt
     5521 gcctctctca ccgaccgaag gagggaacat gcttcgcaag ctcgtcgtac tcaccgtcgc
     5581 cgtcgtcgcc gtcctcgcga tcgcgtcgcc cgcgtcggcc gcccggccgg ccaccgcgcc
     5641 ggacgtcaag tgcctgcaga ccggcctcgg cgtcctcaag gcgaacggcg gcgtcgccta
     5701 cttcgcgaag aacggcgtgc cgctctcgct gatcggcggc gaggggaccg ccccgctctc
     5761 gaccgtgctc aagctccacc tgttccagcc gtcgctcttc ccctggtgcg cgcgctaggg
     5821 cgccctgtgc ccgcggcccg ggcctaggct cgggccgcgg cgcgcctact cgggcccggg
     5881 cgccgcgccg tccgaggtga cgacgcagag cacctggccc gtcgagaccg cgtcaccggc
     5941 gctcacgctg aggttcgtga cgatcccgtc gcggtgcgcg cggatctcgt tctccatctt
     6001 catcgcctcg acgacacaga cgacgcggcc ggcctcgacc tcgtcccctt cggcgacctc
     6061 gacggcgagg acagtgccct gcatcgggct gaccaccgcc tccttcgccg ccgcgtgggc
     6121 gccgccgtgg ctcggcgagc gctcgcgccg gcgccgggcg agctcggcat ggggcggctc
     6181 cggctcgagc acggtcacgt cgtagcggcg cccgtcgagc tcgaccgcga cggcgcgacc
     6241 gcgcagcgca ccgtccgggg ctctcgctac ggtcgttgcc gtatgagaca actcctgcga
     6301 gcgctgcgcg agctcctccg actccacgat gccgtggcac gtcgatcccg cggcgaagca
     6361 cggatgctcg aggagggcgc gatggaagcc gatgagcgtc gtcaccccct cgacgacgta
     6421 ctcctcgagc gcgcggagca tccgccgccg cgcgcgttcg cggtcgaggt cccagacgca
     6481 gagcttcgcg accatcgggt cgtatgtcgc ggagatctcc gagccggcct cgaccccgga
     6541 gtcgacgcgg atccccggcc cggccggctc gcggtaggcg gtgatcgtgc ccggcgtcgg
     6601 gaggaacccg ttcgcggcgt cctcggcgtt gatccggcac tcgatcgcgt ggccgcggaa
     6661 cgttacgtcg tcctggccgt acgcgagcgg ctcgccggcc gcgacgagga tctgctcgcg
     6721 cacgaggtcg atcccggtca cgagctcggt caccgtgtgc tcgacctgga tccgcgtgtt
     6781 catctcgagg aagtagtagg cgccctcggg gtcgagcagg ccttcgatcg tgccggcgcc
     6841 gcgatacccg accgcgcgcg ccgcgtcgag cgcgatctcc ccgatccgtg cgcgaagctc
     6901 gggcgagacg gcgggcgacg gcgtctcctc gacgagcttc tggtgccggc gctggatcgt
     6961 gcagtcgcgc tcgccgaggt gcacggcgtt cccgtgcgcg tcggcgagga cctgcacctc
     7021 gacgtgccgc ggatcgagga tgagcttctc gacgtagacg gtcgggtcgg agaagtacgc
     7081 ctcccc
//
LOCUS       DASTFC010000174         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_415462,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000174 DASTFC010000000
VERSION     DASTFC010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415462"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VFO88_05225"
     CDS             complement(<1..141)
                     /locus_tag="VFO88_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET9544957.1"
                     /translation="MRSGFVAVAGRPNVGKSTLVNALCGGKVAIVSDKPQTTRRRISG
                     IAN"
     gene            complement(138..500)
                     /locus_tag="VFO88_05230"
     CDS             complement(138..500)
                     /locus_tag="VFO88_05230"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET9544958.1"
                     /translation="MTDRELYERAVEAAANAYAPYSNFHVGAALLLRDGRVVTGANVE
                     NASYPLGVCAERAALSRAVAEGVKPGDVEAIGITASPCGGCRQWLLEFRLDRVAFMHE
                     GELLVRTPSELVPDSFEL"
     gene            complement(497..1327)
                     /locus_tag="VFO88_05235"
     CDS             complement(497..1327)
                     /locus_tag="VFO88_05235"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET9544959.1"
                     /translation="MTARRTPTRSPATASAPERERPEAAPAEPELGLAPDVGTKKRPH
                     SLLKSFNYAIEGVIHVLRRERNMRVHFALATVILVLAFAYDVTKVELMILLVAISFVL
                     IAEMVNTAVEEMIDVATTGYDPRAKIAKDVAAGAVLVASVVAATIGYLVFVDRIRSPT
                     YHLVNEVRAIPANLTIIALALTVIAVIAVKAISGRGTPLSGGLPSGHAALAFGGWMAI
                     TLVAADFQHRVLISTLGFLMAFLVAQTRVESGIHTTLEVTIGAVLGTLVTLVLFQTLT
                     "
     gene            complement(1324..1701)
                     /gene="ybeY"
                     /locus_tag="VFO88_05240"
     CDS             complement(1324..1701)
                     /gene="ybeY"
                     /locus_tag="VFO88_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET9544960.1"
                     /translation="MIAVEVENRSGARIDEHAAATLARETLAAEGVEEGDLGLAFVGA
                     DEMRALKREHLGIDEPTDVLSFPIDGREPLAEGVPRQLGDVVLCPDVVGEEWRWPLVH
                     GLLHLLGYDHGDEMARRERELVG"
     gene            complement(1698..>2366)
                     /locus_tag="VFO88_05245"
     CDS             complement(1698..>2366)
                     /locus_tag="VFO88_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9544961.1"
                     /translation="SKKITPKTVNQKRYVDAIRKGTVTFGLGPAGTGKTYLAMALAVA
                     ALSERQVNRIILTRPAVEAGERLGFLPGDMLAKVDPYLRPLYDALYDMLDADRVNSYM
                     ERGTIEVAPLAFMRGRTLNDSFVILDEAQNTTPEQMHMFLTRLGFGSKIVVTGDVTQI
                     DLPREQTSGLVRVREILAAVEGIEFVELGNEDVVRHVLVQRIVEAYKRHAENGGEPEP
                     EQGP"
BASE COUNT          344 a          799 c          880 g          343 t
ORIGIN      
        1 gttcgcgatg cccgagatgc ggcggcgggt cgtctgcggc ttgtccgaga cgatcgccac
       61 cttgccgccg cagagcgcgt tcacgagcgt cgacttgccg acgttcgggc ggccggcgac
      121 ggcgacgaac cccgacctca cagctcgaag gagtccggca cgagctccga gggggtgcgg
      181 acgaggagct cgccctcgtg catgaacgcg acgcggtcga gccggaactc gagcagccac
      241 tggcggcagc cgccgcacgg cgaggcggtg atcccgatcg cctcgacgtc acccggcttg
      301 accccttcgg ccacggcgcg cgagagcgcg gctcgctccg cgcagacccc gagcggatac
      361 gaggcgttct cgacgttcgc gccggtcacg acgcgcccgt cccgcaggag gagcgccgcg
      421 cccacgtgga agttcgagta gggcgcgtac gcgttcgcgg cggcctcgac cgcccgctcg
      481 tagagctcgc ggtcggtcac gtgagcgtct ggaagaggac gagggtgacg agcgtcccga
      541 gcaccgcacc gatggtgacc tcgagcgtcg tgtggatgcc ggactcgacg cgcgtctggg
      601 cgacgaggaa cgccatcagg aagccgaggg tcgagatcag cacgcgatgc tggaagtccg
      661 cggcgacgag cgtgatcgcc atccagccgc cgaacgcgag cgcggcgtgg ccggacggga
      721 gcccgcccga gagcggcgtc ccgcggccgg agatcgcctt cacggcgatg accgcgatca
      781 cggtcagcgc gagcgcgatg atcgtgaggt tcgcggggat cgcccgcacc tcgttgacca
      841 ggtggtacgt cggcgagcgg atccggtcga cgaagacgag atagccgatc gtcgcggcga
      901 cgacggaggc gacgagcacg gccccggcgg ccacgtcctt cgcgatcttc gcgcgcgggt
      961 catagccggt cgtcgcgacg tcgatcatct cctcgaccgc cgtgttcacc atctcggcga
     1021 tgaggacgaa cgagatcgcg acgagcagga tcatgagctc gaccttcgtg acgtcgtagg
     1081 cgaaggcgag gacgaggatc acggtcgcga gcgcgaagtg gacgcgcatg ttgcgctcgc
     1141 gccggaggac gtggatgacg ccctcgatcg cgtagttgaa gctcttgagg agggagtgcg
     1201 gacgcttctt cgtgccgacg tcgggggcga gtccgagctc gggctccgcc ggagcggcct
     1261 cggggcgctc gcgctccggt gcggaggccg tggccggcga gcgcgtgggc gtccggcggg
     1321 cggtcatccg acgagctccc gctcgcggcg ggccatctcg tcgccgtggt cgtagccgag
     1381 caggtggagc agcccgtgga cgaggggcca gcgccactcc tcgccgacga cgtcggggca
     1441 gaggacgacg tcgccgagct ggcgcgggac gccctcggcg agcggctcgc ggccgtcgat
     1501 cgggaaggag agcacgtcgg tcggctcgtc gatcccgagg tgctcgcgct tcagcgcccg
     1561 catctcgtcc gcgccgacga acgcgagccc gaggtcgcct tcctcgacgc cctcggcggc
     1621 gagcgtctcg cgcgcgagcg tcgcggcggc gtgctcgtcg atccgcgcgc cgctgcggtt
     1681 ctcgacctcg acggcgatca cggcccctgc tccggctccg gctcgccgcc gttctcggcg
     1741 tggcgcttgt acgcctcgac gatccgctgg acgagcacgt ggcggacgac gtcctcgttc
     1801 ccgagctcga cgaactcgat cccctcgacc gcggcgagga tctcgcggac gcggacgagg
     1861 cccgacgtct gctcgcgcgg gaggtcgatc tgcgtcacgt caccggtcac aacgatcttc
     1921 gagccgaagc cgagccgcgt caggaacatg tgcatctgct cgggcgtcgt gttctgcgcc
     1981 tcgtcgagga tcacgaacga gtcgttgagc gtgcggccgc gcatgaacgc gagcggggcg
     2041 acctcgatcg tgccgcgctc catgtacgag ttgacgcggt cggcgtcgag catgtcgtag
     2101 agcgcgtcgt agagcgggcg caggtacggg tcgaccttcg cgagcatgtc gccggggagg
     2161 aacccgagcc gttcgccggc ttcgaccgcc gggcgcgtga ggatgatccg gttcacctga
     2221 cgctcggaga gcgccgcgac cgcaagggcc atggcgaggt aggtcttgcc cgtgccggcc
     2281 ggcccgagcc cgaacgtgac cgtgcccttg cggatcgcgt cgacgtagcg cttctggttc
     2341 accgtcttcg gggtgatctt cttcga
//
LOCUS       DASTFC010000175         8904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_36509,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000175 DASTFC010000000
VERSION     DASTFC010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36509"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFO88_05250"
     CDS             complement(<1..762)
                     /locus_tag="VFO88_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9544962.1"
                     /translation="MSLVAELERICGREHVLTDRDRLRTYESDGLLHLRERPAVAALP
                     GTAEEVRRVVAACHAAETPWVARGAGTGLSGGALPVADGVLLVLSRLRRILSVDLPNG
                     RVTCEPGVANLAVSRAVAPTHFFPPDPSSQVVCTVGGNVAENSGGAHCFKYGFTTNYV
                     TGLEVVLPDGDVVELGGAELDRPGLDLVGAFVGSEGTLGVATRITLRVVPVPETIRTL
                     VAYFETMEDAGRTVSDVVASGFVPGALEIRDRLSLR"
     gene            complement(772..1716)
                     /locus_tag="VFO88_05255"
     CDS             complement(772..1716)
                     /locus_tag="VFO88_05255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET9544963.1"
                     /translation="MPPVVAVTRRVVPSVRDELGRRFDLRIHDSEQPALRDELVALVA
                     GADGLLTMLTDAVDAELLDAAGPQLRVVANHATGHDNVDLAAARERGVTVTTTPGVLE
                     RATAEHTIALLLALTRRVAEGDRFLRRGEPWAWAPTFMLGHGLAGRTLGLVGLGRIGA
                     EVARLAAALGLEVVYTARTPRDVPYPRLPLGELLAGADAVSLHVPLTDETRGLIGAEE
                     LGLMRPGAVLVNTARGPIVDEAALAGALRAGEIAGAALDVFEHEPAVHPGLLELENVV
                     LTPHTASATHEAREAMGRLCVEALTAVLLDGRTPDTAV"
     gene            complement(1746..2048)
                     /locus_tag="VFO88_05260"
     CDS             complement(1746..2048)
                     /locus_tag="VFO88_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544964.1"
                     /translation="MADVWTVPLPSDAPASFDVFVNGVLQERGRDYEVAGRTLRFRKP
                     LAREGRLGVMRWASMFLGVAGTYRKNDSVDVVYEVGGKRKVATGLPIYAPDAERPA"
     gene            2259..3272
                     /gene="coxB"
                     /locus_tag="VFO88_05265"
     CDS             2259..3272
                     /gene="coxB"
                     /locus_tag="VFO88_05265"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9544965.1"
                     /translation="MPRTVLVFLALALPAAAVVAALFLPGIAPPRTVTDSGRTIHEIY
                     WVTFALAALVFILVEAALLAFIFRFRRRRDMPDGLEGPQIHGNTRVEIIWTAIPAVLL
                     LALAVYAISRVPDVEATPPASASGEVLEIEVTARQFYWQYEYPNGAISYDALYVPVDT
                     HVTLFLRSTDVAHSWWVPDLSGKRDAIPGRLNRINFRATETGFFEHGVCGEFCGTQHA
                     RMTTDAHVLSAEEFEQWLADNEPPLEDDATVALGEAQWAAACAKCHGLAGEGGYGPPL
                     AGNTTLVTEDALRQIVTEGQDLDANPGYMPPVGRGWTDEQIRALIAYVESNPDLAPQG
                     GSR"
     gene            3272..4906
                     /gene="ctaD"
                     /locus_tag="VFO88_05270"
     CDS             3272..4906
                     /gene="ctaD"
                     /locus_tag="VFO88_05270"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET9544966.1"
                     /translation="MAVDVPTAARPRPAWNEGPVLSWLTTTDHKRIGILYIVTAFLFF
                     LLGGVMALLIRIQLMQADSDFITEDAYNALFTMHGTTMIFLFIVPVLAGFANYLVPLM
                     IGAQDMAFPRLNAMTYWLFLAGGLVLMGSFFAPGGAAKSGWYAYPPISWQYPNVGQDL
                     WILGIHLTTIASIAGAINFIVTIHNMRAPGMTWMRIPLFVWTIEAYAVLLLLALPTLT
                     AAVTLLLLDRQAGTSFFLPDEGGSAVLYQHMFWFFGHPEVYIMILPAFGMVSEIIPVF
                     SRKPIFGYKAIAFSTIGIAFASMLVWAHHMFTVGLPTWLNAFFAVTSFSVAVPTAIKI
                     FNWIATLWRGQLQFKAPLMFCLGLIAFFTMGGLSGILVATFPFDYQAHDSYYIVAHMH
                     YVLFGGTAFGLFAGSYYWFPKITGRMYNERLAQWHFWLILIGFNMTFFFQHMVGLLGM
                     PRRVYTYSRGGSFELYNLLSTIGTWVMTVAILLFLWNMIKSWRTGPRVGPDPWKADTL
                     EWYADSPPAPHNFTSVPPVTSARPLRDLRMRIKEQR"
     gene            4903..6147
                     /locus_tag="VFO88_05275"
     CDS             4903..6147
                     /locus_tag="VFO88_05275"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET9544967.1"
                     /translation="MTGAPRPGSWLRLLAVSAAFATAVVVVTGPWSRSHEASVLAAFG
                     LLAGVWLSVWFGHRASRGLVWTSSVALGLFVVQIGIGGIVALGGESTVALALHIGLGA
                     VSLAAAAVTAGLAVRRGGADAPPAPPAPWRDYVTLTKPRIMSLLLLTGAGGLVLGEGG
                     VPSLGLTLATLGGLALACGGASALNHYLDRDIDKLMGERTQARPVASGRVAPARALEF
                     GLALLALSFVCLVVFANVLAAVLAIFGGAFYVLVYTRWLKRTTPQNIVIGGAAGAIPP
                     VVGWAAATGNLTLPALLLFLIVFFWTPPHFWALALLIRRNYEAAGVPMLPVVRGDRET
                     ARAIVRYSVVMVAITLLPVLARTVGAVYLASALVLGALFLWLAVALARSTTPLAARRL
                     FTYSLAYLALLFVALAVDPLVV"
     gene            6343..7440
                     /locus_tag="VFO88_05280"
     CDS             6343..7440
                     /locus_tag="VFO88_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9544968.1"
                     /translation="MRLEAAVEEGIPLAVKDLFDTAGLRTTYGSGIFAGHVPDRTASA
                     VTRLVEAGCVVVGKTGLHEFAYGITSENEHFGDVLNPLDRTRRPGGSSGGSAVALVAD
                     MADAALGTDSGGSIRIPAAACGVVGFKPTWGRVPTDGVFPLAPSFDHAGPLARTVGEC
                     VPLARALDPDLALPEADPRDLRVGLAWTELAAPLVRARVGDAAAAFGAVERLNFPFPD
                     GVYPVFMREVAEVHRELFAEHRDRYGASVRPKIERCLATTEEEAATAERARERYREAA
                     RGALGDLDLLVAPTMTTVAQPTGVPEHELREEAIRLTFPFNALGWPVLALPCGPAEHG
                     LPASLSVIGRPGDDALVLAAGLGLERARSPV"
     gene            7484..>8904
                     /locus_tag="VFO88_05285"
     CDS             7484..>8904
                     /locus_tag="VFO88_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544969.1"
                     /translation="MLSPRILVVLLVALVAAPAAQAQTAPQNVRAFLLRANEPEPAGR
                     TFARTPSFAWGRVSGATRYHFQLSTSRRFTENAVVWEAKKLPSPLTSIPLTLPWITGD
                     PYSLYARVRAFSGSSAGPWSARYGFRMKSPGPPASLSSGVNPRPGMVRWTPVEGATAY
                     QVTFLYDLGAGEKKLIKTGTTAADLREYYTFHNDLQTRGIDQVSWRVRAVRELEGTPK
                     NDLPVVSYGPWSPTFVTQEPPTIDPVQIALQESISRSRGSNIVNTVLDPGPDPHELVP
                     GFRWTGRYSADGLGDCAAVVAAAPGLTCPLYHVYVFTDEDCVNRVHASDLVGSPAYVP
                     RLSKPLELPASPDRLAEAVDFFLGDTQLDEGAVYDAGGDEVYAAGLHPSVPVDPEDEN
                     LPEGQVPDRKSGIWDNDWPESRYYWTVVPAVPRITPDNQVGYHDVEFAEDNCQAGDVV
                     QFGKTSAPAVERASGVPYVSGMLA"
BASE COUNT         1150 a         3345 c         3108 g         1301 t
ORIGIN      
        1 gcggagcgag aggcggtccc tgatctcgag cgcgcccggg acgaagccgg aagcgaccac
       61 gtcggagacg gtgcgtcccg cgtcctccat cgtctcgaag taggcgacga gcgtgcgaat
      121 cgtctccggc accggcacga ctcggagcgt gatgcgggtc gcgacgccca gggtcccctc
      181 gctcccgacg aacgccccga cgaggtcgag gcccggccgg tcgagctccg cgccgccgag
      241 ctcgacgacg tcgccgtccg ggaggacgac ctcgagcccg gtgacgtagt tcgtcgtgaa
      301 cccgtacttg aagcagtgcg cgccgcccga gttctccgcg acgttcccgc cgacggtgca
      361 gacgacctgg ctcgaagggt cgggcgggaa gaagtgcgtg ggcgcgacgg cccgcgagac
      421 ggcgaggttc gcgacgcccg gctcgcacgt gacgcggccg ttcgggaggt cgacggaaag
      481 gatgcgccgc aggcgcgaga ggacgagaag gacgccgtcc gcgaccggca gcgccccccc
      541 ggagaggccc gtgccggcgc cgcgcgcgac ccagggcgtc tccgccgcgt ggcaggcggc
      601 gacgacgcgg cgcacctcct ccgccgtgcc gggaagagcc gcgacggcgg gccgctcccg
      661 caggtggagg agcccgtccg actcgtacgt gcggagccgg tcgcggtcgg tgaggacgtg
      721 ctcgcgcccg cagatccgct cgagctccgc gacgaggctc atgcgcggag cctagacggc
      781 ggtgtcgggg gtgcgaccat cgaggaggac ggccgtgagc gcctcgacgc agagccgccc
      841 catcgcctcg cgggcctcgt gcgtcgcgct cgccgtgtgc ggggttagga cgacgttctc
      901 gagctcgagc aggccggggt ggacggcggg ctcgtgctcg aagacgtcga gcgcagcgcc
      961 ggcgatctcg cccgcgcgga gcgccccggc gagcgccgcc tcgtcgacga tcgggccgcg
     1021 tgccgtgttc acgaggacgg ctcccggccg catgaggccg agctcctcgg cgccgatgag
     1081 tccgcgcgtc tcgtcggtga gcggcacgtg gaggctgacg gcgtcggcgc cggcgagcag
     1141 ctcgccgagc ggaagccttg ggtacgggac gtcgcgcggc gtgcgcgcgg tgtagacgac
     1201 ctcgaggccg agcgcggcgg cgagccgggc gacctccgcg ccgatgcggc cgaggccgac
     1261 gagcccgagc gtccggccgg cgagcccgtg cccgagcatg aacgtcggcg cccacgccca
     1321 cggttcgcca cggcgcagga agcggtcgcc ctcagcgacc cggcgcgtga gcgcgaggag
     1381 gagcgcgatc gtgtgctcgg ccgtcgcacg ctcgagcacc ccgggcgtcg tcgtgacggt
     1441 gacgccgcgc tcgcgggccg ccgcgaggtc gacgttgtcg tggccggtcg cgtggttcgc
     1501 gacgacccgg agctgcgggc cggccgcgtc gaggagctcc gcgtcgacgg cgtcggtgag
     1561 catcgtgagg agcccgtcgg cgcctgcgac gagcgcgacc agctcgtcgc gcaacgccgg
     1621 ctgctccgag tcgtggatcc tgaggtcgaa gcgccgcccg agctcgtcgc gcaccgaggg
     1681 gacgacgcgg cgggtgacgg cgacgacggg cggcacgccg ggagcctaag cggccgggcc
     1741 gcgagctacg ccggccgctc cgcgtccggc gcgtagatcg gaagcccggt cgcgaccttg
     1801 cgcttcccgc cgacctcgta caccacgtcg acggagtcgt tcttccggta cgtcccggcc
     1861 acgccgagga acatgctcgc ccaccgcatg acgccgagcc gcccctcgcg cgcgagcggc
     1921 ttgcggaacc ggagcgtccg ccccgcgacc tcgtagtcgc gcccgcgctc ctgcagcacg
     1981 ccgttcacga agacgtcgaa cgacgcaggc gcgtcggaag ggagcggaac agtccagacg
     2041 tcggccaccg cagcgccgaa gcctagaccc ccgctccctt cctcgcgcca gacccgcttc
     2101 gctctcccct gctgctacgg ctctcccgtc caaggcagtt gccgtccctc ctactgaacc
     2161 acagaaaggg ggaaactcaa tccctcgaac gagcgagtcc gcacgagccg cgcaggccgg
     2221 accgcccgcg gccgcgagcg gccttagaat cggcccggat gccgcgcacg gtcctcgtct
     2281 tcctcgcgct cgccttgccg gccgccgcgg tcgtcgcggc actcttcctg cccgggatcg
     2341 caccgccccg gacggtcacg gacagcgggc ggacgatcca cgagatctac tgggtgacgt
     2401 tcgcgctcgc ggcgctcgtc ttcatcctcg tcgaggcggc gctcctcgcg ttcatcttcc
     2461 gcttccggcg ccgccgcgac atgccggacg gcctcgaggg cccgcagatc cacgggaaca
     2521 cacgcgtcga gatcatctgg acggcgatcc cggccgtgct cctcctcgcc ctggcggtct
     2581 acgcgatctc gcgcgtgccc gacgtcgagg cgacgccgcc cgcctcggcc tccggcgagg
     2641 tgctcgagat cgaggtgacg gcccggcagt tctactggca gtacgagtat ccgaacggcg
     2701 cgatctccta cgacgcgctc tacgtcccgg tcgacacgca cgtgaccctg ttcctccgct
     2761 cgaccgacgt cgcccactcc tggtgggtgc cggacctgag cgggaagcgc gacgcgatcc
     2821 cggggcgcct gaaccggatc aacttccgcg ccaccgagac cggcttcttc gagcacggcg
     2881 tctgcggcga gttctgcggc acgcagcacg cgcgcatgac gaccgacgcg cacgtcctct
     2941 ccgcggagga gttcgagcag tggctcgccg acaacgagcc gccgctcgaa gacgacgcga
     3001 ccgtcgccct cggcgaggcg cagtgggcgg cggcgtgcgc gaagtgccac gggctcgccg
     3061 gcgagggcgg ctacgggcct ccgctcgccg gcaacacgac gctcgtgacg gaggacgccc
     3121 tgcgccagat cgtcaccgag ggccaggacc tcgacgcgaa ccccggctac atgccgccgg
     3181 tcggccgcgg ctggacggac gagcagatcc gggcgctcat cgcgtacgtc gagtcgaacc
     3241 ccgacctcgc cccgcaagga gggagccgct gatggccgtc gacgtcccga ccgccgcccg
     3301 cccgcgcccg gcctggaacg agggccccgt cctcagctgg ctgacgacga ccgaccacaa
     3361 gcggatcggg atcctctaca tcgtcaccgc gttcctcttc ttcctcctgg gcggcgtgat
     3421 ggcgctgctc atccggatcc agctcatgca ggcggactcg gacttcatca ccgaggacgc
     3481 ctacaacgcg ctcttcacga tgcacgggac gacgatgatc ttcctcttca tcgtcccggt
     3541 gctcgccggc ttcgcgaact acctcgtccc gctcatgatc ggcgcgcagg acatggcgtt
     3601 cccgcgcctg aacgcgatga cctactggct cttcctcgcc ggcgggctcg tgctcatggg
     3661 gagcttcttc gccccagggg gcgccgcgaa gtccggctgg tacgcgtacc cgccgatctc
     3721 ctggcagtac ccgaacgtgg gccaggacct ctggatcctc ggcatccacc tgacgacgat
     3781 cgcctcgatc gcgggcgcga tcaacttcat cgtgacgatc cacaacatgc gcgcgcccgg
     3841 gatgacctgg atgcgcatcc cgctcttcgt gtggacgatc gaggcgtacg cggtgctcct
     3901 cctcctcgcg ctgccgacgc tcaccgcggc ggtcacgctg ctcctgctcg accgccaggc
     3961 cgggacgagc ttcttcctcc cggacgaggg cggcagcgcg gtgctctacc agcacatgtt
     4021 ctggttcttc gggcaccccg aggtctacat catgatcctg cccgcgttcg ggatggtctc
     4081 ggagatcatc ccggtgttct cgcgcaagcc gatcttcgga tacaaggcga tcgcgttctc
     4141 gacgatcggg atcgcgttcg cgtcgatgct cgtctgggcg caccacatgt tcacggtcgg
     4201 cctcccgacc tggctgaacg ccttcttcgc cgtcacttcg ttctcggtcg cggtgccgac
     4261 ggcgatcaag atcttcaact ggatcgcgac gctctggcgc gggcagctcc agttcaaggc
     4321 gccgctcatg ttctgcctcg ggctcatcgc gttcttcacg atgggcggcc tgagcgggat
     4381 cctcgtcgcc accttcccgt tcgactacca ggcgcacgac tcgtactaca tcgtcgcgca
     4441 catgcattac gtgctcttcg gcgggacggc gttcgggctc ttcgccggct cgtactactg
     4501 gttcccgaag atcaccggcc ggatgtacaa cgagcgcctc gcgcagtggc acttctggct
     4561 catcctgatc ggcttcaaca tgacgttctt cttccagcac atggtcgggc tgctcgggat
     4621 gccgcggcgc gtctacacgt actcgcgcgg cgggtcgttc gagctctaca acctgctctc
     4681 gacgatcggg acgtgggtca tgaccgtggc gatcctgctc ttcctctgga acatgatcaa
     4741 gagctggcgc accgggccgc gcgtcgggcc ggacccgtgg aaggccgaca cgctcgagtg
     4801 gtacgcggac tcgccgcccg cgccgcacaa cttcacgagc gtgccgcccg tgacgagcgc
     4861 gcggccgctg cgcgacctgc gcatgcgaat caaggagcag cgatgaccgg cgcgccccgc
     4921 cccggctcct ggctccggct gctcgccgtc tccgcggcgt tcgcgacggc ggtcgtcgtc
     4981 gtcaccgggc cgtggtcgcg ctcgcacgag gcgagcgtcc tcgccgcctt cgggctgctc
     5041 gccggcgtct ggctgagcgt ctggttcggg caccgggcct cccgcgggct cgtgtggacg
     5101 tcgagcgtcg cgctcgggct cttcgtcgtc cagatcggga tcggcgggat cgtcgcgctc
     5161 ggcggggagt cgaccgtcgc gctcgccctc cacatcgggc tcggcgcggt gtccctcgcg
     5221 gcagcggccg tcaccgcggg gctcgcggtg cgccgcggcg gcgccgacgc gccgcccgcg
     5281 ccgcccgcgc cgtggcgcga ctacgtgacg ctgacgaagc cgcggatcat gtccctcctg
     5341 ctcctcaccg gggcgggcgg gctcgtgctc ggcgagggcg gcgtgccgtc gctcgggctc
     5401 acgctcgcga cgctcggcgg gctcgcgctc gcctgcggcg gcgcctcggc gctgaaccac
     5461 tacctcgacc gggacatcga caagctcatg ggcgagcgga cgcaggcgcg gccggtcgcg
     5521 tcgggccgcg tcgcgccggc gcgggcgctc gagttcgggc tcgcgctcct cgcgctctcc
     5581 ttcgtctgcc tcgtcgtctt cgcgaacgtg ctcgcggcgg tgctcgccat cttcggcggc
     5641 gccttctacg tcctcgtcta cacgcgctgg ctcaagcgga cgacgccgca gaacatcgtg
     5701 atcggcggcg cggcgggcgc gatcccgccc gtggtcggct gggcggcggc gacgggaaac
     5761 ctgacgcttc ccgcgctcct gctcttcctc atcgtcttct tctggacgcc gccgcacttc
     5821 tgggcgctcg cgctcctcat ccggcggaac tacgaggcgg ccggcgtccc gatgctcccc
     5881 gtcgtccgcg gcgaccgcga gacggcgcgg gcgatcgtcc gctactccgt cgtcatggtg
     5941 gcgatcacgc tcctcccggt gctcgcgcgg acggtcgggg ccgtctacct ggcgagcgcc
     6001 ctcgtgctcg gcgcgctctt cctctggctg gcggtcgcgc tcgcgcgctc gacgacgccg
     6061 ctcgccgcgc ggcgcctctt cacgtactcg ctcgcgtacc tcgcgctcct cttcgtcgcc
     6121 ctcgccgtcg accccctggt ggtgtaaccg tggatcccga gctcgcccgc aagaacatcc
     6181 gcctcgggct gatcctgttc gcgctcgtcg tcgtgctctt cgcgggctcg atcgccgtcg
     6241 cggagctgta caacgccttc gccgactgac ctgtcccgac gggtcgaggc cgcgctcgcg
     6301 gcgatccgcg agcgggacgg cgacctgaac gccttcgtca cggtgcggct ggaggcggcc
     6361 gtcgaggagg ggatcccgct cgcggtcaag gatctcttcg acacggcggg gctgcggacg
     6421 acgtacgggt cggggatctt cgcgggccac gtgcccgacc gcaccgcctc cgcggtcaca
     6481 cggctcgtcg aggcagggtg cgtcgtcgtc ggaaagacgg ggctgcacga gttcgcctac
     6541 gggatcacct ccgagaacga gcacttcggc gacgtcctca acccgctcga ccggacacgc
     6601 cgcccgggcg gctcgagcgg cggctcggcg gtggcgctcg tcgcggacat ggcggacgca
     6661 gccctcggca ccgactccgg cggctcgatc cggatcccgg ccgcggcgtg cggcgtcgtc
     6721 ggcttcaagc cgacgtgggg ccgcgtcccg acggacggcg tcttcccgct cgcgccgagc
     6781 ttcgaccacg cggggccgct cgcgcgcacg gtcggcgagt gcgtcccgct cgcgcgggcg
     6841 ctcgacccgg acctcgccct cccggaggcc gacccgcgcg acctgcgcgt cgggctcgcg
     6901 tggacggagc tcgccgcgcc gctcgtccgc gcgcgcgtcg gggacgcggc tgccgcgttc
     6961 ggggcggtcg aacggctgaa cttcccgttc cccgacggcg tgtaccccgt cttcatgcgg
     7021 gaggtggccg aggtccaccg cgagctcttc gccgagcacc gcgaccgcta cggcgcgagc
     7081 gtgcggccga agatcgagcg ctgcctcgcg acgaccgagg aggaggcggc gaccgcggaa
     7141 cgggcccgcg agcgctaccg cgaggccgcg cggggggcgc tcggcgacct cgacctgctc
     7201 gtcgcaccga cgatgacgac ggtcgcccag cccaccggcg tgccagagca cgagctccgg
     7261 gaggaggcga tccggctcac cttcccgttc aacgcgctcg gctggccggt cctcgcgctc
     7321 ccgtgcgggc ccgcggagca cggccttccc gcctcgctct cggtgatcgg gcgccccggc
     7381 gacgacgcgc tcgtcctcgc cgcggggcta ggcctggagc gggcccgctc acccgtttga
     7441 gggacagccg ggcaccgggt cgtgccgaca cacactggga gccatgctct cccctcgcat
     7501 tctcgtcgtg ctcctcgtgg cgctcgtcgc cgcgcccgcg gcccaggccc agacggcgcc
     7561 gcagaacgtg cgcgcgttcc tgctgcgcgc gaacgagccc gagccggccg gccggacgtt
     7621 cgcccgcacg ccctccttcg cgtggggccg cgtgagcggc gcgacgcgct accacttcca
     7681 gctctccacg agccgccggt tcaccgagaa cgcggtcgtc tgggaggcga agaagctccc
     7741 gtcgccgctc acctcgatcc cgctcacgct gccgtggatc acgggcgacc cgtactccct
     7801 ctacgcgcgc gtgcgcgcgt tctccggcag ctcggccggg ccgtggagcg cgcgctacgg
     7861 cttccggatg aagtcgcccg gcccgccggc gagcctctcc tcgggcgtga acccgcggcc
     7921 cgggatggtg cgctggacgc ccgtggaggg cgcgaccgcc taccaggtca cgttcctgta
     7981 cgacctcggc gcgggcgaga agaagctgat caagacgggc acgaccgcgg ccgacctgcg
     8041 cgagtactac acgttccaca acgacctcca gacgcgcggg atcgaccagg tctcgtggcg
     8101 cgtccgcgcc gtccgggagc tcgagggcac gccgaagaac gacctcccgg tcgtctcgta
     8161 cggcccgtgg agcccgacct tcgtgaccca ggagccgccg acgatcgacc ccgtgcagat
     8221 cgcgctccag gagtcgatct cgcggagccg cggctcgaac atcgtgaaca ccgtgctcga
     8281 cccgggcccc gacccgcacg agctcgtccc cggcttccgg tggacggggc gctactccgc
     8341 cgacgggctc ggcgactgcg ccgcggtcgt cgccgccgcc cccgggctca cctgcccgct
     8401 ctaccatgtg tacgtcttca cggacgagga ctgcgtgaac cgcgtgcacg cctccgacct
     8461 cgtcggcagc ccggcgtacg tgccgcggct ttcgaagccg ctcgagctcc ctgcgtcgcc
     8521 ggaccggctc gcggaggcgg tcgacttctt cctcggggac acgcagctcg acgagggcgc
     8581 cgtctacgac gcgggcggcg acgaggtgta cgcggccggg ctccatccga gcgtccccgt
     8641 ggaccccgag gacgagaacc tccccgaggg ccaggtgccc gaccgaaaga gcggcatatg
     8701 ggacaacgac tggcccgaga gccgctacta ctggaccgtc gtcccggccg ttccgcgcat
     8761 cacgccggac aaccaggtcg ggtaccacga cgtggagttc gcggaggaca actgccaggc
     8821 gggcgacgtg gtgcagttcg gcaagaccag cgcgccggcc gtcgagcggg cgagcggcgt
     8881 gccgtacgtg tccgggatgc tcgc
//
LOCUS       DASTFC010000176         7873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_695607,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000176 DASTFC010000000
VERSION     DASTFC010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695607"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFO88_05290"
     CDS             complement(<1..197)
                     /locus_tag="VFO88_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544970.1"
                     /translation="MAAVATAAIAVAGSGLAVAMTRGLFEATPAPPEVVENFSLYTPQ
                     LGFDPLPEAAELVAADGEFRL"
     gene            complement(281..841)
                     /locus_tag="VFO88_05295"
     CDS             complement(281..841)
                     /locus_tag="VFO88_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9544971.1"
                     /translation="MTDATDAEALRDSRRDPEAVCVLYDRHAERLLAALVRTTGDREL
                     AFDVVQETFARALERGHRVRLSPDGSAWPWLWRVARNLAADARRRGAVDRRARVRLGM
                     AVVALDDAYEEVDKRVDAVALGPPLAAAIAALPPGERDAVTGRVSDGLSYDALARSLG
                     TTEVAARARVSRGLRALRLRLSGGRP"
     gene            complement(935..1636)
                     /locus_tag="VFO88_05300"
     CDS             complement(935..1636)
                     /locus_tag="VFO88_05300"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HET9544972.1"
                     /translation="MSLVAAIHQPNYLPWPGYFHKLAAADVFVHLDAVQFPRGQSFAA
                     RNRIKTPNGVTFLTVPVTAPKDEEGTFTYLDAGFADDRWRKKHLRTVEQAYGKAPHFE
                     AVFALYRREVEGAETFVGLTIGLVEAVAGYLGIETRCVRLSELLPGGFGQKTELIVDV
                     CRALDADVYLSGSGGGRDYNDEELLRGHGIELRYDEYRYPEHPQLWGDFEPNLSVLDV
                     LMNCGPAARPLVVGE"
     gene            complement(1633..2613)
                     /locus_tag="VFO88_05305"
     CDS             complement(1633..2613)
                     /locus_tag="VFO88_05305"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9544973.1"
                     /translation="MGERFEELYEKRRGAYAKDASDEEFLRRLNEDLAEREEALYEDV
                     GVERPVVFVVGLPRSGTTLLSQLLAYCLDAGYVTNVAARFWLAPVHGIRLSRVLAGDE
                     PPASFESDYARTKTLTGIHEFGYFWRRWLRKDTFEDVARSAEREDEIDWAGLRRTLGN
                     VQAELGRPLVAKNMLGAYHMPRLRRELGPVLWVEIERDPLDVAVSILDARRSYYDDPS
                     AWWSYVPLEIEELRGLDPWEQVAGQVHYLSRLYARAEAELPPDGFLRVRYEELARDPA
                     AVLEAVRARTGIPLRREPPASFELRRHEGREEEKERFAALLERFAEKDAA"
     gene            2668..3807
                     /locus_tag="VFO88_05310"
     CDS             2668..3807
                     /locus_tag="VFO88_05310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9544974.1"
                     /translation="MATQRTRRETLLGFQPPAIGEEEIEAVVETLRTGWIATGPKAGE
                     LEERFAEYVGARHAVTLASGTAALHLALLAAGIGPGDEVITTPITWPATANVIVHAGA
                     TPVFVDVRAEDLNVDPDAVAEAIGPRTKAIIPVHLAGQPCDLDALLALGAPVIEDATH
                     AVESVYRGRKIGSIEGSLCAAFSLNATKNVAGAEGGILTTDDDGLAAEIRELRLMRRK
                     PGALYDIRVAGFKANLPDLLAALALVQLEKVEEHAAIRRRHVAAYDEAVAELDGITPL
                     ARDPRDVHALHIYVVRIDPERAGADRNAYRDALAEERIATSIHFLPLHRLTFYRGLLP
                     EQPPLPVAERAGDEVLSLPLSPAHSDEDVADAVDALRRVHAAFSS"
     gene            3812..4771
                     /locus_tag="VFO88_05315"
     CDS             3812..4771
                     /locus_tag="VFO88_05315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9544975.1"
                     /translation="MAPDLTVSIVNASRRDTLLPLLASLEGEDAEVVVLDNASGDGSV
                     EAVRERFPGVRVIEQPFRAGFGANHNRVIRETEGHYVLLLSHDAEPEPGAVRTLVAFA
                     DAHPRAGAVAPRLRFPDGRVQASAWRFPTPAVAALGTLTLARAGVVQSRGEAPRRVDW
                     AMGAALLLRREALDDAGLFDERYFMYSEETDLCLRLARAGWETWFVPAAVVVHHDSAL
                     RAELPRERIAEEWRSRHRYWRTHHSAAGARVAAALTGAQYAARAGIAAAVRRARPGAY
                     DPSFPARMRLHARSAWRGPQGPGLRERVDEWNRAHAARESEPA"
     gene            4768..6120
                     /locus_tag="VFO88_05320"
     CDS             4768..6120
                     /locus_tag="VFO88_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9544976.1"
                     /translation="MTRGTRTTDVLLFVTAFVVTFTKVRFTTPAGDIYVSDLAATLFI
                     VAVLASRLYARDWSLPRTAAIAAAFFGVFLIVFLVGFYNLETAADRDLYVKGLVKFVI
                     HFAFVVVAVAYLIRRSQRLYWQTLGWFLAGFVANAVYGVFQLGFAELAGRNLDELVLG
                     TFGLYERGGIGVFGVAGSGANVYRTNALTLDSNNLSVMLCVPVLIGLAFYLRLPRGHR
                     LRVPLALVLSFLVLVMLTTLSRSGMLGLAAGLLVLAIPYHHLFLRPRVLVPVGALALV
                     LAAVVAARFDFFTEVFQARTQTSGTSTQTHFLFYKLVAPAFEAHPMFGMGLNTFATYY
                     EFHTGREDYGPHSWYVAVVTETGVAGAIAFAAWIVFLFRRLADLHRVGRRLAAARDRL
                     ADRVRPLAWGLVAALVGTMAANIFYLTMQMYYVFVFATLVLAAPAVFSRGEPGRDGVA
                     "
     gene            6129..7253
                     /locus_tag="VFO88_05325"
     CDS             6129..7253
                     /locus_tag="VFO88_05325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9544977.1"
                     /translation="MRIALLDPPGFTPPYDHALAAALAARGHDVALFTAPDLFGSARP
                     PEGYVRRETFLPLSGRLLRGRGRSRARLVVKGLEYVPSVVGSVRAVRRYGPDVVHVQW
                     LGIPRFDVRWLERLARTFPTALTAHDVLPRKARNAEGWGKALGLVDRVVVHSDRAVER
                     LAELGVARPRLVRILHPAFETAGDPPGEPSGRTLLFFGLLRRYKGLDVLLRAMPAVLR
                     AVPDARLVVAGDPLEPAEPLVELARELGLDGAVEWRLRFIEEPELGELFADAALTVLP
                     YRQLDSSGVLATALGAGRPAVVTDVGSLGAIVAEHGAGRVVPPESPEALAEAIVELLA
                     DPAALAQAAEGARRARGALTWEASAEAHERLYAELVAERS"
     gene            7250..>7873
                     /locus_tag="VFO88_05330"
     CDS             7250..>7873
                     /locus_tag="VFO88_05330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9544978.1"
                     /translation="MTGYERHRVQRAVKALADPLAAAVLLVVLSPLVGAVALWTLLDA
                     GRPVLFVSPRAGRGGVTFRMLKFRTMIPDAIAEGRRLALTPDPFGLVPDDPRITRSGR
                     FLRRTGLDEIPQLVNVIRGEMSLVGPRPDLVEQAEHYTEEEARRLVAKPGITGWSQIH
                     GREDQTWADRFAYDAWYLEHWSLWLDARILLRTVGQLFRPEPVPVVDP"
BASE COUNT          951 a         2993 c         2838 g         1091 t
ORIGIN      
        1 tacaggcgga actcgccgtc ggcggcgacg agctcggccg cttcgggaag cgggtcgaac
       61 ccgagctgcg gcgtgtacag ggagaagttc tcgaccacct ccggcggcgc cggcgtcgcc
      121 tcgaagagcc cgcgcgtcat cgcgaccgcg aggccgctgc ccgcgacggc gatcgccgcg
      181 gtcgcgacgg ccgccacgac ccgtcgccgt cgccgccgtc gcgtgaggcg acgcgccgac
      241 gcggcgcgca gctcggcacg gaagagggcg agcgcgtcgc tcacggacgg cctcccgaga
      301 gccggaggcg cagggcacgg aggccgcgcg aaacgcgagc gcgcgccgcg acctcggtcg
      361 tgccgagcga gcgcgcgagc gcgtcgtagg agagcccgtc cgagacgcgc ccggtgaccg
      421 cgtcgcgctc gccgggcggc agggccgcga tcgccgcggc gaggggcggt ccgagcgcga
      481 cggcgtccac acgcttgtcg acctcctcgt acgcgtcgtc gagggccacg acggccatcc
      541 cgaggcggac gcgcgcgcgc cggtcaaccg ccccgcgccg gcgggcatcg gcggcgagat
      601 tgcgcgcgac gcgccagagc cacggccagg cggacccgtc cggggacagc cgcacccggt
      661 ggccgcgttc gagcgcccgc gcgaacgtct cctggacgac gtcgaacgcg agctcccggt
      721 cgccggtagt ccggacgagc gccgcgagca gccgctctgc gtgccggtcg tagagcacgc
      781 agaccgcctc ggggtcgcgc cgagagtcgc gcagcgcctc agcgtccgtg gcgtcggtca
      841 tctctgagag aaacgttcga gtgcaacgaa gtgtgacagc gattcgggcc acggagccgg
      901 ccgcgactcg agctcgcttg ccggggccgg gttcctactc cccgacgacg agcgggcgcg
      961 ccgcgggccc gcagttcatg agcacgtcga ggacggagag gttcggctcg aagtcgcccc
     1021 agagctgcgg gtgctcggga taccggtact cgtcgtagcg gagctcgatc ccgtgcccgc
     1081 ggagcagctc ctcgtcgttg tagtcgcgcc cgccgccgga gcctgagagg tagacgtcgg
     1141 cgtccagcgc gcggcagacg tccacgatca gctccgtctt ctggccgaag ccgccgggca
     1201 ggagctccga gagccgcacg caccgggtct cgatcccgag gtagcccgcg accgcctcga
     1261 cgaggccgat cgtgaggccg acgaacgtct cggcgccctc gacctcgcgc cggtagagcg
     1321 cgaagaccgc ctcgaagtgc ggcgccttcc cgtacgcctg ctcgaccgtg cggagatgct
     1381 tcttgcgcca gcggtcgtcc gcgaagccgg cgtcgaggta ggtgaacgtg ccctcctcgt
     1441 ccttcggcgc ggtcaccggg acggtgagga acgtgacgcc gttcggcgtc ttgatccggt
     1501 tgcgcgcggc gaagctctgc ccgcgcggga actgcaccgc gtcgaggtgg acgaagacgt
     1561 ccgcggccgc gagcttgtgg aagtagccgg gccaggggag gtagttcggc tggtggatcg
     1621 cggcgacgag gctcacgccg cgtccttctc cgcgaagcgc tcgaggagcg cggcgaagcg
     1681 ctccttctcc tcctcgcgcc cctcgtggcg gcggagctcg aaggacgccg gcggctcgcg
     1741 ccgcagcggg atgccggtgc gcgcccgcac cgcctcgagc accgcggcgg gatcgcgcgc
     1801 gagctcctcg tagcgcacgc gcaggaagcc gtccggcggg agctccgcct cggcgcgcgc
     1861 gtacaggcgc gagaggtagt gcacctgccc ggcgacctgc tcccacgggt cgaggccgcg
     1921 gagctcctcg atctcgagcg ggacgtagct ccaccacgcg ctcgggtcgt cgtagtagct
     1981 ccggcgcgcg tcgaggatcg agacggcgac gtcgagcggg tcgcgctcga tctccaccca
     2041 gagcacgggc ccgagctccc ggcgcaggcg cggcatgtgg tacgcgccga gcatgttctt
     2101 cgccacgagc ggccgcccga gctcggcctg cacgttcccg agcgtccgcc ggaggcccgc
     2161 ccagtcgatc tcgtcctcgc gctcggccga gcgggccacg tcctcgaacg tgtccttgcg
     2221 gagccagcgg cgccagaagt agccgaactc gtggatcccg gtgagcgtct tcgtgcgcgc
     2281 gtagtccgac tcgaacgacg cgggcggctc gtccccggcg agcacgcgcg agagccggat
     2341 cccgtgcacc ggcgcgagcc agaagcgcgc ggcgacgttc gtcacgtagc cggcgtcgag
     2401 gcagtacgcg aggagctggc tcaggagcgt cgtccccgag cgcggcagcc cgacgacgaa
     2461 gacgaccggc cgctcgacgc cgacgtcctc gtagagcgcc tcttcccgct cggcgagatc
     2521 ctcgttcagc cggcggagga actcctcgtc ggacgcgtcc ttcgcatacg ccccgcggcg
     2581 cttctcgtag agctcttcga accgctcgcc catccgggcc ggagcgtact tccgcggcag
     2641 cccggccggg cgcataccct ttccgccgtg gcgacgcagc gcacgcgacg cgagaccctg
     2701 ctcggcttcc agccgcccgc gatcggcgag gaggagatcg aggcggtcgt cgagaccctc
     2761 cggaccggct ggatcgcgac cgggccgaag gcgggcgagc tcgaggagcg cttcgcggag
     2821 tacgtcggcg cgcgccacgc ggtcaccctc gcgtcgggga cggcggcgct ccatctcgcg
     2881 ctcctcgcgg ccggcatcgg ccccggggac gaggtgatca cgaccccgat cacgtggccc
     2941 gcgacggcga acgtgatcgt ccacgccggc gcgacgcccg tcttcgtcga cgtgcgcgcg
     3001 gaggatctga acgtcgaccc ggacgccgtc gccgaggcga tcgggccgcg gacgaaggcg
     3061 atcatccccg tccacctcgc cggccagccg tgcgacctcg acgcgctcct cgcgctcggg
     3121 gccccggtga tcgaggacgc gacgcacgcc gtcgaatcgg tctaccgcgg gcgcaagatc
     3181 gggtcgatcg agggctcgct ctgcgccgcg ttctccctca acgcgacgaa gaacgtcgcg
     3241 ggcgcggagg gcgggatcct gacgacggac gacgacgggc tcgccgcaga gatccgcgag
     3301 ctgcgcctga tgcgccgcaa gccgggcgcg ctctacgaca tccgcgtggc cgggttcaag
     3361 gcgaacctcc ccgacctcct cgccgcgctc gcgctcgtcc agctcgagaa ggtcgaggag
     3421 cacgccgcga tccgccgccg ccatgtcgcc gcctacgacg aggcggtcgc cgagctcgac
     3481 gggatcacgc cgctcgcgcg cgacccgcgc gacgtccacg cgctccacat ctacgtcgtc
     3541 cgcatcgacc ccgagcgcgc gggcgccgac cggaacgcct accgcgacgc gctcgccgag
     3601 gagcggatcg cgacctcgat ccacttcctc ccgctccacc ggctcacgtt ctaccgcggg
     3661 ctcctgcccg agcagccgcc gctcccggtc gccgagcggg cgggcgacga ggtgctctcg
     3721 ctgcccctct cgccggcgca ttcggacgag gacgtggcag atgccgtcga cgcgctccgg
     3781 cgcgtgcacg ccgcgttctc ctcgtaggcc ggtggcgccc gacctcacgg tctcgatcgt
     3841 gaacgcctcg cggcgggaca cgctcctgcc gcttctcgcc tcgctcgagg gcgaggacgc
     3901 ggaggtggtc gtcctcgaca acgcctccgg cgacggctcg gtcgaggcgg tgcgcgagcg
     3961 ctttcccggc gtccgcgtga tcgagcagcc attccgcgcc gggttcggcg cgaaccacaa
     4021 ccgcgtgatc cgggagacgg agggccacta cgtcctcctc ctctcgcacg acgccgagcc
     4081 ggagccgggc gcggtgcgaa cgctcgtggc gttcgcggac gcacacccgc gggccggagc
     4141 ggtcgcgccg cgcctgcgct ttccggacgg gcgcgtccag gcgagcgcgt ggcgcttccc
     4201 gacgcccgcg gtcgccgcgc tcgggacgct cacgctcgcg cgcgccggcg tcgtccagtc
     4261 gcgcggcgag gcgccgcggc gggtggactg ggcgatgggc gccgccctgc tcctccgccg
     4321 ggaggcgctc gacgacgccg ggctcttcga cgagcgctac ttcatgtact cggaggagac
     4381 cgacctctgc ctccgcctcg cccgcgccgg ctgggagacg tggttcgtcc ccgcggcggt
     4441 cgtcgtgcac cacgacagcg ccctgcgggc cgagcttccg cgcgagcgga tcgcggagga
     4501 gtggcgctcg cggcaccgct actggcggac gcaccactcg gccgccggcg cgcgggtcgc
     4561 cgccgccctc accggggcgc agtacgccgc ccgcgcgggg atcgccgccg ccgtgcggcg
     4621 cgcgcggccg ggcgcgtacg acccgagctt cccggcccgg atgcgcctgc acgcccgctc
     4681 ggcctggcgc ggcccgcagg ggccgggcct ccgggagcgc gtcgacgagt ggaaccgcgc
     4741 gcacgccgcg cgagaatcgg agccggcatg acgcgcggca cgcgcacgac cgacgtcctc
     4801 ctcttcgtga ccgcgttcgt cgtcacgttc acgaaggtcc gcttcacgac cccggcgggc
     4861 gacatctacg tctcggacct cgcggcgacc ctcttcatcg tggccgtcct cgcgagccgc
     4921 ctctacgcgc gcgactggtc gctcccgcgg acggcggcga tcgcggcggc gttcttcggc
     4981 gtcttcctga tcgtcttcct cgtcgggttc tacaacctcg agacggccgc ggatcgcgac
     5041 ctctacgtga aggggctcgt caagttcgtc atccacttcg cgttcgtcgt cgtcgccgtc
     5101 gcgtacctca tccggcgctc gcagcggctc tactggcaga cgctcggctg gttcctcgcc
     5161 gggttcgtgg cgaacgcggt ctacggcgtc ttccagctcg gcttcgcgga gctcgccggg
     5221 cgcaacctcg acgagctcgt gctgggcacg ttcggcctct acgagcgcgg cgggatcggc
     5281 gtgttcggcg tcgcggggag cggggcgaac gtctaccgga cgaacgcgct cacgctcgac
     5341 tcgaacaacc tctcggtgat gctctgcgtc cccgtcctga tcgggctcgc gttctacctg
     5401 cgcctgccgc gcgggcacag gctgcgcgtg ccgctcgcgc tcgtgctctc gttcctcgtg
     5461 ctcgtcatgc tgaccaccct ctcgcggagc gggatgctcg ggctcgccgc cgggctcctc
     5521 gtgctcgcga tcccgtacca ccacctcttc ctgcgcccgc gcgtcctcgt cccggtcggt
     5581 gcgctcgccc tcgtgctcgc agcggtcgtc gccgcccggt tcgacttctt cacggaggtc
     5641 ttccaggcgc gcacgcagac gagcgggacg tccacgcaga cccacttcct gttctacaag
     5701 ctcgtcgcgc ccgcgttcga ggcgcacccg atgttcggga tgggcctgaa cacgttcgcc
     5761 acgtactacg agttccacac cggacgcgag gactacgggc cgcactcgtg gtacgtcgcc
     5821 gtcgtgacgg agacgggcgt ggccggcgcg atcgcgttcg ccgcgtggat cgtcttcctc
     5881 ttccggcggc tcgcagacct gcaccgcgtc ggccgccggc tggccgccgc ccgcgaccgt
     5941 ctggccgacc gggtgcggcc gctcgcctgg gggctcgtcg ccgcgctcgt cgggacgatg
     6001 gccgcgaaca tcttctacct cacgatgcag atgtactacg tgttcgtctt cgcgaccctc
     6061 gtgctggccg cgccggcggt gttctcgcgc ggggagccgg gccgcgacgg ggtagcgtga
     6121 cgcctcccgt gcgtatcgcg ctcctcgacc cgcccggctt cacgccaccc tacgaccacg
     6181 ccctcgccgc ggcgctcgcc gcacgcggcc acgacgtcgc cctcttcacc gcgcccgacc
     6241 tcttcggctc ggcccgcccg ccggagggct acgtgcggcg ggagacgttc ctcccgctga
     6301 gcggccggct cctgcgcgga cgcggccgct cgcgcgcgcg cctcgtcgtg aaggggctcg
     6361 agtacgtgcc gagcgtcgtg ggctccgtcc gggcggtacg gcgctacggg ccggacgtcg
     6421 tgcatgtcca gtggctcggg atcccgcgct tcgacgtccg ctggctcgag cggctcgccc
     6481 ggacgtttcc gaccgcgctc accgcgcacg acgttctgcc gcgcaaggcc cggaacgcgg
     6541 aggggtgggg gaaggcgctc gggctcgtgg atcgcgtcgt cgtccactcc gaccgcgcgg
     6601 tcgagcggct cgcggagctc ggcgtcgcgc gcccgcggct cgttcggatc ctgcatccgg
     6661 ccttcgagac cgccggcgac ccgcccggcg agccgagcgg gaggacgctt ctcttcttcg
     6721 ggctgctgcg ccggtacaag gggctcgacg tcctcctccg cgcgatgccg gcggtgctcc
     6781 gcgccgtccc ggacgcgcgg ctcgtcgtcg cgggcgaccc gctcgagccg gccgagccgc
     6841 tcgtcgagct cgcgcgggag ctgggcctcg acggcgcggt cgagtggcgg ctgcggttca
     6901 tcgaggagcc ggagctcggg gagctcttcg ccgatgctgc gctcacggtg ctcccgtacc
     6961 ggcagctcga ctcctccggc gtgctcgcga ccgcgctcgg ggccgggcgg cccgcggtcg
     7021 tcacggacgt ggggtcgctc ggcgcgatcg tcgccgagca cggcgccggc cgggtcgtgc
     7081 cgcccgagag cccggaggcc ctcgccgagg cgatcgtcga gctcctcgcc gacccggccg
     7141 ccctcgcgca ggcggcggag ggcgcgcgcc gggcgcgcgg ggcgctcacg tgggaggcgt
     7201 cggccgaggc gcacgagcgg ctctacgcgg agctcgtcgc ggagcggtcg tgaccgggta
     7261 cgaacggcac cgcgtccagc gcgccgtgaa ggcgctcgcc gatccgctcg ccgcggccgt
     7321 gctcctcgtc gtgctctcgc cgctcgtcgg cgcggtcgcg ctctggacgc tcctcgacgc
     7381 cggccggccc gtgctcttcg tgagcccgcg cgcggggcgc ggcggcgtga cgttccggat
     7441 gctgaagttc cgcacgatga tcccggacgc gatcgccgag ggacggcgcc tcgcgctcac
     7501 gcccgatccg ttcggccttg tccccgacga tccgcggatc acgcgcagcg gccgcttcct
     7561 gcgccgcaca gggctcgacg agatcccgca gctcgtgaac gtcatccgcg gggaaatgag
     7621 cctcgtcggg ccgcgccccg acctcgtcga gcaggccgag cactacacgg aggaggaggc
     7681 ccgccgcctc gtcgcgaagc ccggcatcac cggctggtcg cagatccacg ggcgggagga
     7741 ccagacgtgg gccgaccgct tcgcctacga cgcctggtat ctcgagcact ggtccctctg
     7801 gctcgacgcg aggatcctcc tccgcacggt cgggcagctc ttccggccgg agcccgtgcc
     7861 ggtcgtcgac ccg
//
LOCUS       DASTFC010000177         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_959331,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000177 DASTFC010000000
VERSION     DASTFC010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959331"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFO88_05335"
     CDS             <1..559
                     /locus_tag="VFO88_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544979.1"
                     /translation="REREPRRRRRRRVPWPLLAVLLVALAGGAAIWAIADGRIDPSGG
                     NGNAAPADEIRLQAARDHDPIGADREHPEAVARATDGDPATYWPTETYTSFTKEGVGL
                     VVDAGRPVELGGLTVVTDEPGWTALIQAGTRAQGPFEAVSEEQRVGRETTFDVDTGGE
                     AYRFYVVWITGLEGRAIVNEIRAAD"
     gene            complement(556..1515)
                     /locus_tag="VFO88_05340"
     CDS             complement(556..1515)
                     /locus_tag="VFO88_05340"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET9544980.1"
                     /translation="MRDGRLRVAVLMGGRSSEHEVSLNSGSSVVEGLSPERYDVVPVE
                     IPREGPWLLTREEAAAYDVVFPVLHGPFGEDGTVQGLLELADVAYVGAGVAGSALAMD
                     KDLFKAVMRDNGVPVARSQTLRRGRDEGGESPFGYPVVVKPARLGSSVGISIVRSAEE
                     LRPAVELAFRHDEKVLVEEFLEGVEVECSVLGNEDPVPSVPGEIVPLASDWYDYEAKY
                     AEGGMELVVPARIPPETTARVQELAVAAFVAADCEGMARVDFFAAPDGAVTVNELNTI
                     PGFTATSVYAKLFEASGLPYGELLDRLVALALERHERRRALRF"
     gene            complement(1512..>2024)
                     /locus_tag="VFO88_05345"
     CDS             complement(1512..>2024)
                     /locus_tag="VFO88_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9544981.1"
                     /translation="LRVSQGQETAIVPDVLQQSEESAIAELAAAGFEASVTSEPSNTV
                     AEGLVISQAPEPGEEAPVGSTVEIVVSDGPSSVTVPDVRTLTQEDAEAELEARDFQVQ
                     VEDQETDDPTQEGRVVAQNPAPGVQAAPGSTVTIFVARLVDLDGAPSNERAGPEPPGE
                     AIGLRRRSAL"
BASE COUNT          279 a          740 c          747 g          258 t
ORIGIN      
        1 acgggagcgc gagccgaggc gccgccgcag gcgccgggtg ccgtggccgc ttctcgcggt
       61 gctgctcgtc gcgctcgcag gcggcgcggc gatctgggcg atcgcggacg ggcgcatcga
      121 cccgagcggg gggaacggca acgcggcgcc ggccgacgag atccggctcc aagcggcacg
      181 ggaccacgat ccgatcggcg cagaccgaga gcacccggag gccgtggcgc gtgccaccga
      241 cggcgatccc gcgacgtact ggccgaccga gacgtacacg tcgttcacga aggagggcgt
      301 cggcctcgtc gtcgacgcgg gccgcccggt cgagctcggc ggactgaccg tcgtgaccga
      361 cgagccgggc tggacggcgc tgatccaggc cggcacgcgc gctcagggcc cgttcgaggc
      421 ggtctcggag gagcagaggg tcgggcgcga gacgacgttc gacgtcgaca cgggcggcga
      481 ggcataccgg ttctacgtcg tctggatcac cggcctcgag ggccgcgcga tcgtgaacga
      541 gattcgcgcc gccgactaga acctgagcgc gcggcgccgc tcgtggcgct cgagcgcgag
      601 cgcgacgagc cggtcgagaa gctcgccgta cgggaggccg gaggcctcga agagcttcgc
      661 gtacacgctc gtcgccgtga acccgggaat ggtgttcagc tcgttcacgg tcacggctcc
      721 gtcgggcgcg gcgaagaagt cgacgcgggc catgccctcg cagtcggccg ccacgaaggc
      781 ggcgaccgcg agctcctgca cgcgcgcggt cgtctccggg gggatccgcg ccgggacgac
      841 gagctccatt ccgccctcgg cgtacttcgc ctcgtagtcg taccagtcgc tcgcgagcgg
      901 gacgatctcg ccgggcacgg atgggaccgg gtcctcgttc ccgagcaccg agcactcgac
      961 ctcgacgccc tcgaggaact cctcgacgag gaccttctcg tcgtgcctga acgccagctc
     1021 gaccgcgggg cgaagctcct cggccgagcg gacgatcgag atcccgacgc tcgagccgag
     1081 gcgggccggc ttcacgacga ccgggtagcc gaacgggctc tcgccgccct cgtcgcggcc
     1141 gcgccgcagc gtctgggagc gggcaacggg gacgccgttg tcgcgcatca cggccttgaa
     1201 gaggtccttg tccattgcga gggcggagcc ggcgacgccc gcgccgacgt aggcgacgtc
     1261 cgcgagctcg aggaggccct gcaccgtccc gtcctcaccg aacggcccgt gcagcaccgg
     1321 gaagacgacg tcgtaggccg ccgcctcctc gcgcgtcagg agccacggcc cctcgcgcgg
     1381 gatctcgacc gggacgacgt cgtagcgctc cggggagagc ccctcgacga ccgagctgcc
     1441 cgagttgagc gagacctcgt gctcgctcga gcggccgccc atgaggacgg cgacgcggag
     1501 ccgcccgtcc ctcacagggc cgagcggcgt cgcaggccga tcgcctcgcc gggcggttcc
     1561 ggcccggcgc gctcgttcga gggggccccg tccagatcca cgaggcgggc gacgaagatc
     1621 gtcaccgtgc tccccggcgc cgcctgcacg ccgggcgcgg ggttctgggc gacgacgcgg
     1681 ccctcctgcg tcgggtcgtc cgtctcctgg tcctcgacct gcacctggaa gtcgcgggcc
     1741 tcgagctcgg cctcggcgtc ctcctgcgtc agcgtgcgca cgtcggggac ggtcaccgag
     1801 gacggaccgt cggagacgac gatctccacg gtcgagccga ccggcgcctc ctcgcccggc
     1861 tcgggggcct ggctgatgac caggccttcg gcgaccgtat tgctcggctc gctcgtgacc
     1921 gacgcctcga acccggccgc ggcgagctcg gcgatcgcgc tctcctccga ctgctggagg
     1981 acatcgggca cgatcgccgt ctcctggccc tgcgagacgc gcag
//
LOCUS       DASTFC010000178         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_926326,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000178 DASTFC010000000
VERSION     DASTFC010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926326"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFO88_05350"
     CDS             <1..892
                     /locus_tag="VFO88_05350"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9544982.1"
                     /translation="IHSTVLAPDGRRMSKSLGTGIDPMDMIEKHGADATRYGLLKMSS
                     TQDVRFAEGMIEEGRKLANKLWNVARLILGNAEGVEPAARPETLEERWIFARLDAATR
                     EVKEALRAFEFSRAVQGLYRLTFDDFCDWYAEAAKPLLYERDPRATATALAALERLLA
                     LLHPVLPHVTEEIWTALPAREARLVVSPWPEPSGEWDGAAGALERVQDAAAIYRRSGV
                     RVALEPEEERIFAAVVRPAEANGGGDARAEIERLRKEVARGERMLSDARFVENAPADV
                     VEAEREKLARYQRELDALER"
     gene            876..1979
                     /locus_tag="VFO88_05355"
     CDS             876..1979
                     /locus_tag="VFO88_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET9544983.1"
                     /translation="MPSNADWLAGLELFGVRLGLERTVALLERLGRPERGLRAIHVVG
                     TNGKTSVVRGAEALLLAEGLSVGAYTSPDVTGWAERIRVDGEEADVETVLGRVRAAAE
                     EVGATQFEVLTAAALAAFADARVDVAVVEAGLGGRLDATNVLGAPVVCLTNVALEHTE
                     HLGETREAIAAEKLAVVPPGATVVLGEPEWEGDALARGAARVVAAEGNEALARACAEA
                     FLGRKVAAEAAPVAVPGRIEHVADAPLELWDGAHNPAGVAYLAERLPARRDWTLVLSI
                     LGDKDVDAMLAAFSAFGPRLVATSSGHPRALSAGELARRAACFFRRVDVEPDPLVARE
                     RAREAAGPDGAVLVSGSLTIVAALDAVRPATVR"
     gene            2104..2595
                     /locus_tag="VFO88_05360"
     CDS             2104..2595
                     /locus_tag="VFO88_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9544984.1"
                     /translation="MQAQEETDDAFGSVRDFFDSSIWLLFRNVSLFFIAIFWLASAFW
                     VYKDAKRRIEDPWLLAMAVALGIFPPFLGPIIYMLFRPPEYLEDVRERELEIKAMEES
                     LLGERCPVCRADVDPSYLACPVCTTKLKQACRGCKAPLEPLWQICPYCETPVETPRAV
                     ENF"
     gene            2607..2828
                     /locus_tag="VFO88_05365"
     CDS             2607..2828
                     /locus_tag="VFO88_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544985.1"
                     /translation="MSLALLLPLAQHTNAGPSTGGHGELVMTIMIVVVLSVIWTALGV
                     VCWIFWRAKKREDAARAEAEVEWRNAPSS"
     gene            2804..3220
                     /gene="ndk"
                     /locus_tag="VFO88_05370"
     CDS             2804..3220
                     /gene="ndk"
                     /locus_tag="VFO88_05370"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET9544986.1"
                     /translation="MAERTLVLIKPDAVERRLAGEILARLERRGLALRGAKLVQVSRE
                     LAEEHYAEHTEKPFFGELVEFITSSPTLALVVEGESAIAVVRTTMGATNPVDSAPGTI
                     RGDLALAMPNNLVHGSDSKESADREVALWFSEDELV"
     gene            3213..3983
                     /locus_tag="VFO88_05375"
     CDS             3213..3983
                     /locus_tag="VFO88_05375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9544987.1"
                     /translation="MSDELTEHARKNRAYWDGVSDSYQDENAEFITHTELHWGMWQIS
                     DSELGILGSVAGKDVFELGCGAAQSGIVLARAGARVVGLDNSARQLEHARRAVEAAGV
                     ELELVHGSAESLPFADASFDVVFADHGANRFADPYLWVPEAARVLRPDGLLAFSGGTP
                     FEVICFDEAADTWAPRLERPYFGMHAYTFGEDSAVEFDLPYGEWIRVFREAGLSVEVL
                     VEVRPPEGAASTYRNAAETEFARSWPMEQIWKARKTPA"
     gene            4133..>4622
                     /locus_tag="VFO88_05380"
     CDS             4133..>4622
                     /locus_tag="VFO88_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9544988.1"
                     /translation="MAGKDVVELGCGTAYFSAWLARRGARPVGVDVTPEQLATARALQ
                     AEHGLAFPLVEASAEDVPLPSESFDLALSEYGASIWADPYRWVPEAARLLRPGGELVF
                     LVHGLLLALCWPNFDDPAGEELARAYFGLRSLEDPADGATTFHLGCGEWIRLLRANGL
                     EVE"
BASE COUNT          588 a         1632 c         1750 g          652 t
ORIGIN      
        1 catccactcg accgtcctcg cgccggacgg gcggcgcatg tcgaagagcc tcggcaccgg
       61 gatcgacccg atggacatga tcgagaagca cggcgccgac gcgacccgct acgggctcct
      121 gaagatgtcc tcgacgcagg atgtccgctt cgcggagggg atgatcgagg agggccgcaa
      181 gctcgcgaac aagctctgga acgtcgcgcg gctcatcctc gggaacgccg agggcgtcga
      241 gccggccgcg cggccggaga cgctcgagga gcgctggatc ttcgcccgcc tcgacgcggc
      301 gacccgcgag gtcaaggagg cgctgcgcgc gttcgagttc tcgcgcgccg tgcagggcct
      361 ctaccggttg accttcgacg acttctgcga ctggtacgcg gaagcggcga agccgctcct
      421 ctacgagcgc gacccgcgcg cgacggcgac ggcgctcgcg gcgctcgagc ggctgctcgc
      481 gctcctgcac cccgtgctcc cgcacgtgac ggaggagatc tggaccgcgc ttcccgcgcg
      541 cgaggcgcgg ctcgtcgtct cgccctggcc cgagccgagc ggcgagtggg acggcgccgc
      601 gggcgcgctc gagcgggtgc aggacgcggc cgcgatctac cggcggagcg gcgtccgcgt
      661 cgcgctcgag cccgaggagg agcggatctt cgccgccgtc gtgcgcccgg ccgaggccaa
      721 cggcggcggc gacgcgcgcg cggagatcga gcggctgcgc aaggaggtcg cccgcggcga
      781 gcggatgctc tccgacgcgc gcttcgtcga gaacgcgccc gccgacgtgg tcgaggccga
      841 gcgcgagaag ctcgcccgct accagcgtga gctcgatgcc ctcgaacgct gactggctcg
      901 cggggctcga gctcttcggc gtcaggctgg ggctcgagcg gacggtcgcg ctgctcgagc
      961 gcctgggacg gcccgagcgc gggctgcgcg cgatccacgt ggtcgggacg aacgggaaga
     1021 cgagcgtcgt ccgcggggcc gaggcgttgc tcctcgccga ggggctctcc gtcggcgcgt
     1081 acacctcgcc cgacgtgacc ggctgggcgg agcggatccg cgtggacggg gaggaggcgg
     1141 acgtcgagac cgtgctgggg cgcgtgcgcg cggccgccga ggaggtcggc gcgacgcagt
     1201 tcgaggtgct gacggccgcc gcgctcgccg cgttcgcgga cgcccgcgtg gacgtcgccg
     1261 tcgtcgaggc ggggctcggc gggcggctcg acgccacgaa cgtcctcggc gcgcccgtcg
     1321 tctgcctgac gaacgtcgcg ctcgagcaca ccgagcacct gggcgagacg cgcgaggcga
     1381 tcgccgcgga gaagctcgcg gtcgtcccgc ccggcgcgac ggtcgtcctc ggggagcccg
     1441 agtgggaggg cgacgcgctc gcgcgcgggg cggcccgcgt cgtcgcggcg gaggggaacg
     1501 aggcgctcgc gcgcgcgtgc gccgaggcgt tcctcgggcg caaggtcgct gcggaggccg
     1561 cgccggtcgc cgtccccggc cgtatcgagc acgtcgcgga cgccccgctc gagctctggg
     1621 acggcgcgca caacccggcc ggcgtcgcgt acctcgcgga gcggctcccg gcgcggcgcg
     1681 actggacgct cgtcctctcg atcctcggcg acaaggacgt cgacgcgatg ctcgccgcgt
     1741 tctccgcgtt cgggccgcgc ctcgtcgcca cctcgtccgg ccacccgcgc gcgctctccg
     1801 ccggcgagct ggcccgtcgt gcggcctgct tcttccgtcg cgtggacgtc gagccggacc
     1861 cgctcgtcgc gcgcgagcgc gcgcgcgagg cggccggccc ggacggtgcg gtgctcgtct
     1921 ccggctccct gacgatcgtg gcggcgctcg acgccgtccg gcccgcaacc gtacgatgag
     1981 ccgcgtcagc gagcagatcg gcgtcttcgc cttcgccgcc ctcgtgctcg tcgcgatcgt
     2041 ggcgctggcg tttggcgccg gctgggtggt cggaaagatg ctcctgtgaa cccaatcccc
     2101 ctgttgcagg cacaagagga gaccgacgac gcgttcggct ccgtccggga cttcttcgac
     2161 agctcgatct ggctgctctt ccggaacgtg tcgctcttct tcatcgcgat cttctggctc
     2221 gcgtccgcgt tctgggtcta caaggacgcg aagcggcgga tcgaggaccc gtggctcctc
     2281 gcgatggcgg tcgcgctggg gatcttcccg ccgttcctcg ggccgatcat ctacatgctc
     2341 ttccgcccgc ccgagtacct cgaggacgtg cgcgagcgcg agctcgagat caaggcgatg
     2401 gaggagagcc tcctcggcga gcgctgcccc gtctgccgcg ccgacgtcga cccctcgtac
     2461 ctcgcgtgcc ccgtctgcac gacgaagctc aagcaggcgt gccgcggctg caaggcgccg
     2521 ctcgagccgc tctggcagat ctgcccgtac tgcgagacgc cggtcgagac gcccagggcg
     2581 gtcgagaact tctagtctca ggggcgatgt cgctcgccct cctcctgccg ctcgcgcagc
     2641 acacgaacgc gggcccctcg accggcgggc acggcgagct cgtgatgacg atcatgatcg
     2701 tcgtcgtcct gtcggtcatc tggaccgccc tcggcgtcgt ctgctggatc ttctggcggg
     2761 cgaagaagcg cgaggacgcg gcccgggcgg aagcggaggt cgaatggcgg aacgcaccct
     2821 cgtcctgatc aagcccgacg cggtcgagcg caggctcgcc ggcgagatcc tcgcgcgcct
     2881 cgagcgccgg gggctcgcgc tccgcggggc gaagctcgtc caggtgagcc gcgagctcgc
     2941 cgaggagcac tacgcggagc acacggagaa gccgttcttc ggcgagctcg tggagttcat
     3001 cacctcgtcc ccgacgctcg cgctcgtcgt cgagggcgag tcggcgatcg cggtcgtccg
     3061 cacgacgatg ggcgcgacga acccggtcga ctcggcgccg gggacgatcc gcggcgacct
     3121 cgcgctcgcg atgccgaaca acctcgtgca cgggtcggac tcgaaggagt cggccgaccg
     3181 cgaggtggcg ctctggttct cggaggacga gcttgtctga cgagctcacc gagcacgcgc
     3241 gcaagaaccg ggcgtactgg gacggcgtct cggactccta ccaggacgag aacgccgagt
     3301 tcatcacgca caccgagctc cactggggca tgtggcagat ctccgactcg gagctcggga
     3361 tcctcgggag cgtggccggc aaggacgtgt tcgagctcgg gtgcggcgcg gcgcagagcg
     3421 ggatcgtcct cgcgcgcgcg ggggcgcgag tcgtggggct ggacaactcc gcccgtcagc
     3481 tcgagcacgc gcggcgggcg gtcgaggcgg cgggcgtcga gctcgagctc gtgcacggga
     3541 gcgccgagtc actgcccttc gcggacgcgt cattcgacgt cgtcttcgcc gaccacggcg
     3601 cgaaccgctt cgccgacccg tacctctggg tgcccgaggc cgcacgcgtt ctccgtccgg
     3661 acgggctgct cgcgttcagc ggtgggaccc cgttcgaggt gatctgcttc gacgaggccg
     3721 ccgacacgtg ggcgccgcgg ctcgagcggc cgtacttcgg gatgcacgcg tacacgttcg
     3781 gcgaggactc ggcggtcgag ttcgacctcc cgtacgggga gtggatccgc gtcttccgcg
     3841 aggcggggct ctcggtcgag gtgctcgtcg aggtgcgccc gcccgagggc gcggcgtcga
     3901 cgtacaggaa cgcggccgag accgagttcg cgcgcagctg gccgatggag cagatctgga
     3961 aggcccggaa gactccggcg tagggctctc ggcgcacgcc gagcgcaacc ggaacgtctg
     4021 ggccgggctc gcccccgagt tcgccgagcg cgggcggcgc aactgggcga agacggagat
     4081 cgactggggg atctgggacg ttcccgaggc gtcgctcggg ctgctcggag acgtggccgg
     4141 gaaggacgtc gtcgagctcg gctgcgggac ggcgtacttc tccgcgtggc tcgcgcgacg
     4201 cggcgcgcgg ccggtcggcg tcgacgtgac gccggagcag ctcgcgacgg cgcgggcctt
     4261 gcaggccgag catgggctcg cgttcccgct cgtcgaggcg agcgccgagg acgtgccgct
     4321 gccgtcggag tcgttcgacc tggccctatc cgagtacggc gcgtccatct gggccgaccc
     4381 gtaccggtgg gtgcccgagg cggcgcgcct cctgcgcccg ggcggcgagc tcgtcttcct
     4441 cgtgcacggg ctcctgctcg cgctctgctg gccgaacttc gacgacccgg ccggggagga
     4501 gctcgcgcgg gcgtacttcg gcctccggtc gctcgaggac ccggcggacg gcgcgacgac
     4561 gtttcacctc ggctgcggcg agtggatccg gctgctgcgc gcgaacgggc tcgaggtcga
     4621 gc
//
LOCUS       DASTFC010000179        18664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_547324,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000179 DASTFC010000000
VERSION     DASTFC010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 18664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18664
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547324"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFO88_05385"
     CDS             complement(<1..703)
                     /locus_tag="VFO88_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9544989.1"
                     /translation="MAKTIGIDLGTTNSCMAVLEGGEPTVIPNAEGGRTTPSVVAFTK
                     DGQRLVGAPAKRQSVTNPENTVSSIKRFMGRKHSEVTEEMTIVPYSVVQGPNGDVRVK
                     AGDKELAPPEISAMILQKLKADAEAYLGEPVTDAVVTVPAYFNNAQREATKDAGKIAG
                     LNVLRIINEPTAASLAYGLDKEADQTILVFDLGGGTFDVSVLELGEGVFEVKSTNGDN
                     HLGGDNFDKAIVDWMV"
     gene            complement(811..2223)
                     /locus_tag="VFO88_05390"
     CDS             complement(811..2223)
                     /locus_tag="VFO88_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9544990.1"
                     /translation="MDQRETPVATAADAYLADERIAPFTTPGHKRSPHLADGALLYDL
                     PLTTAVDDLHSSRGVLARAHALAADLWGADLARFCLNGSTQGNEALALAVGGDGGRIV
                     VSRNLHASLFSGLVLAGLEPVWVRPDIDAATGLPRSVPAARVEDALARAGDARAVFLV
                     EPSYVGVLSDVAAIADAAHARGAPLLVDQAWGAHLGFHPELPQHTLALGADGMVTSAH
                     KTLAAFTQGAYLFGRGGLLDLARLDGAFDMLHTTSPSAAILASLDRTRALMAARGEEL
                     LGRTIALARHAREELEAVEGLTVAATDDPTKLVLALAGTGADGLEVERDLVAAGLPLE
                     LANRDTLVPLLTIGDTEESVGRLVAAVRESVERRRGEPRDPGAASAVWAVEPEVALTP
                     REAFYAPRETVPAERAAGRISAETVAPYPPGIPALAPGEVVSAGIVAALREAAAGGTH
                     LSYAADPTLETLQVVARPPV"
     gene            complement(2306..2476)
                     /locus_tag="VFO88_05395"
     CDS             complement(2306..2476)
                     /locus_tag="VFO88_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET9544991.1"
                     /translation="MADWDQVEGKTKEGVGKATGDEGLEGEGKVQGAWGDTKEGAKEK
                     AGDAVDKVRGDD"
     gene            complement(2513..3475)
                     /locus_tag="VFO88_05400"
     CDS             complement(2513..3475)
                     /locus_tag="VFO88_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9544992.1"
                     /translation="MKVYAALGTIYVVWGSTFLAIEYAVRTLPPFLAMSARHLVAGSL
                     LFAWALWRRPREPIGRAQILAATVFGGALFLGSHGGLAWSQTRIPSGVAALIVASIPL
                     WIAVLDRVAFGKRLSARAVAGLLLGFAGIAALVDPFGGGPIDTVGGLVALLSAGSWAA
                     GSLYARGARLPSDPIASAGLASLAGGVLLLVASAAGGELAGLDPAAIAGESLAGVVYL
                     IVMGSLVGLTAYVWLLRAAPISLVATYAYVNPVVAVLLGWAVVGEEISARVLVAGAAI
                     VVAVALIVSAPAGRADGRGLLRRGVAVEDVSPPASPRSRIRGPG"
     gene            3568..5325
                     /gene="ggt"
                     /locus_tag="VFO88_05405"
     CDS             3568..5325
                     /gene="ggt"
                     /locus_tag="VFO88_05405"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9544993.1"
                     /translation="MRREFLVALAVVAAVALPAPGQVAAAAPPEPVKQATATGTGGAA
                     STVDALATEAALDILDRGGNAVDAAVAAAAVLGVVEPFSCGIGGGGFMVVYSASDRSV
                     TTIDGRETAPGAYTPTTLQGIPFAEAVTSGLGVGVPGTLRTWQAALDRFGTMKLQRLL
                     APAKSIAAGGFLVDQTFHDQTAANAARFGKFTSTSELFLPDGEAPPVGTRFRNPELAK
                     TYAQIGSGPDSFYRGKIAAAIVDAVRTPPVEPGVTDVRPGLMTLADLAAYTAPFRAPT
                     RVGYRGLDVYGMGPPSSGGSTVGEALNILEGYDLAGLSREEALHYYLEASALAFADRN
                     LYLGDPDFVSIPLAGLLSDSYAAERRALIGPTTGVPYPPGDPTDNGGPSTTHLTVSDR
                     HGNVVSYTFTIEQTGGSGIVVPGYGFLLNNELTDFDFVPGRANSPAAGKRPRSSMSPT
                     IVLRDGRPYVAVGSPGGATIITTALQILVNEIDFGMTLPEAIAAPRASQRNLATVQAE
                     PGFIVSPEAAALAARGHRFASTPEIGAATGIAFLPGGFVTAAAEPIRRGGGSAMVQEP
                     TAPMLAGARAKAQRGRALR"
     gene            5382..6368
                     /gene="moaA"
                     /locus_tag="VFO88_05410"
     CDS             5382..6368
                     /gene="moaA"
                     /locus_tag="VFO88_05410"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET9544994.1"
                     /translation="MQLVDGFGRPIRSLRVSVTDKCNFRCTYCMPAEGLEWLRRDEIL
                     SFEEIGRLVRVMAGMGVDEIRLTGGEPLVRRDLPTLVRMLAETPGVDDLSLTTNGVLL
                     DRLAAPLVEAGLRRVNVSLDSLSHVRFAEITRRDALDQVLRGLETAEQHPELRPIKVN
                     CVAVKGFTEEEVPALAELARRKPYVVRFIEFMPLDADRAWAGDKVLTGDEIRALIEER
                     WPLEEIPAKPSSTARRFRFADGAGEVGFVNPVSEPFCSTCDRIRLTADGHLRTCLFSR
                     REWDLKTPLREGATDEELAAAIREAVAHKELKHRINDPGFLPASRSMSQIGG"
     gene            6372..7124
                     /locus_tag="VFO88_05415"
     CDS             6372..7124
                     /locus_tag="VFO88_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9544995.1"
                     /translation="MRERIAAVFPRGPADFALQLGIWFGFLAAYQVARGVADRDVPQA
                     FANGQWIIDFQRATSTLVELDLQRVVLEAGGVLLDVLNWTYWLSQFAVLGVALLWIYF
                     FRTEAFAGVRNWIIAANVIGLLGYVFLPTAPPRMFPGEGFVDTLAHSASLNHGSGLVQ
                     LASNPYAAMPSLHGADALIIGLAMAALVRSWWMKAVWLLWPAWVWFAVMATGNHFWLD
                     IAAGIAVAWVAYALLAWAGRRRDVPRFALSGS"
     gene            7144..7770
                     /locus_tag="VFO88_05420"
     CDS             7144..7770
                     /locus_tag="VFO88_05420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9544996.1"
                     /translation="MNSLERLQGGYIGGVRRLAATAMGRLGRTRVTPNMLTVAGIALS
                     IAGAVVVYFEYAGWGFFWLGAALFVLGAILDILDGALARSRGVASEFGAFVDSTVDRV
                     GEAFMLGAIALVLMREGYEWGVALAFAALAGSFLVSYARARAEALGLKGDVGFGSRAE
                     RVVVITTGLVLAPIHELVLPVTMAILAATAWVTVLQRVLSVRSQLRHR"
     gene            7800..9266
                     /locus_tag="VFO88_05425"
     CDS             7800..9266
                     /locus_tag="VFO88_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET9544997.1"
                     /translation="MARERTEVLVRMPVELKRRLADEVRERGSNLNDVAVEILASRFG
                     IPFDPTGRKGSPGGRSGDVLLRMPRALKERLRDRAEGRRRTTNDLIVETLAERLGLPT
                     KRKDSMSETNGSRNGKRPSGEKVRVALIGVGNCANSLLQGVEFYKDASPEEFVPGLMH
                     VDLGGYHIRDIEFTAAFDVTKDKVGKDLADAIWAHPNDTYRFAEVPKTGIKVSRGMTH
                     DGLGKYISEVVEKAPGDTDPVAEILRETKTDVVVNYLPVGSELATKWYTEQILNAGCA
                     MVNCMPVFIAKGGYFAKQFEQRGLPIIGDDIKSQVGATITHRVLTSLFRERGVHLDKT
                     MQLNVGGNSDFLNMLERDRLESKKISKTNAVTSMLDYDMGADNVHVGPSDYVPWLTDR
                     KWAYIRMEGSSFGNVPLNVELKLEVWDSPNSAGIVIDAVRLAKLALDHGVAGALEGPS
                     AYLMKSPPKQMPDDDAHEAVESFIRKYARKDEPVPASK"
     gene            complement(9316..10167)
                     /locus_tag="VFO88_05430"
     CDS             complement(9316..10167)
                     /locus_tag="VFO88_05430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9544998.1"
                     /translation="MCLPGTAEAVRERIEEEGAPTVTRGTLLAGGAAAAAAAMFPGAA
                     FGGGRGSGGPSRRFKRMQDLTHLFRAGFPVFGGPEPTRETFFDFPTDGFYSQRWTFTE
                     HSGTHMDAPGHFVPGGRLTPEIEADELMRPLVVVDIRRKAASDPNAMVTVDDLRRFER
                     GRGRIPRKAIVAMNSGWDAKVNNSLAFKGAPAFPYNFPGFSGEATEWLIENRDVAGIA
                     VDTLSLDPGNSTTFDAHFAILGADRYGLENVANLGEVPARGATVVVGVVPWEQGSGGP
                     LRAFALW"
     gene            complement(10286..13303)
                     /locus_tag="VFO88_05435"
     CDS             complement(10286..13303)
                     /locus_tag="VFO88_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HET9544999.1"
                     /translation="MGAVALLVPSSGLGVVQLSLDGDDHETFDSRTATLAPTAAQRAA
                     AAALDAEVRWNRFGTPQSVIRYGGHLGDAVAGADAESAARRWVEANATLFRLDSTASL
                     ELESAVPLGGGGAHAVVFRQRIGGLLAAPDGALTVGLTGSAGAGWRVAYASSSLTSDT
                     TLDAAADLSVAEAWVEAADSSGVDASVSDVDVAGRQAGWTQLDVAGVADTQLVRRVAF
                     PTPRRGTIAAYEAYVLDTDEDGHVIGDRVVVDADTGRLLFSESAVHHALDNPKWSVFP
                     SWPRTTPLNRYPWNYPSADTRETWCWLNEAGCRLALAGSSPHPAFAWDVVPPAGVPTF
                     TTTGNYARSAENWMDFLFPGPTQYRPTSPTRDYVYPWENVWFEERCDPDVLVVGSGND
                     IDAATVNLFAMHSRMHNWSYHLGFTESAWNAQLHNFGVGTAQGDPLIGMVQAGAVSHG
                     PPTYAGRDNAFMRTLPDGTPSQSAMFLWQPIPGAFYAPCVDGDYDMAVIGHEYGHMIE
                     NRMIGKGSNRMGFHAGAMGESFGDFVSMEHLNEYNLVPGGDLAWAVGVYATGNHSRAI
                     RNYNMAWPSAGAFPAPGREVRVNPLNFGAVGYDIVGPQVHADGEIWSATNKGIRDLYV
                     DRYGQGGGRLQQECADGDRPPEQCPGNRRWIQTVFDAMLLMPTAPTFLDARDAYLAAD
                     LLRFGGANQDLLWLGFARRGFGQNATTTGTQDTEPVASYASPLHGEATLTFEAVAKDE
                     GGAPIANARVFVGHYEARATPIGATSDFVPSPQGYDFVAQAPGYGHVRFHVRNLRAGE
                     SRTITIHMPTNWASQAKGATASGDGANHANLIDDTEATTWQSTGAPVEGRRVLVTLGG
                     ERTFDLARASALLVPGQNRFVALRSFELHACTAGGGANPTCDPAVAGGWNLFLTAEDA
                     FPADNPRPVAPALNLRTWAVPGTTATHVLFRVLDNQCTGQTSYQGKQDADPRHETDCR
                     IGTPLPPRDNQVVAAELELHSSMPRVDGAQIVE"
     gene            13501..15282
                     /locus_tag="VFO88_05440"
     CDS             13501..15282
                     /locus_tag="VFO88_05440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9545000.1"
                     /translation="MGLRISMVTPFAWSQPHAVNEHVAGAAEELRRRGHEVVVLAPSN
                     RAADLAAGRRALRRLARDGEPLPDLVALGPAVPGPARTLVGVPLGARANLALALSADR
                     FDVVHAHEPGLPSLSYLALRHARGLTVATYHSPERLAYPPGKRQREKLLARVDALTAT
                     RDDTLALARDRFPGDFRRLPFGVDTARFRPGRERKRFVLEWRANETARATAAFRALAD
                     LPGWELVVLRTRPLSGRPYVPRAVRGRVHVARGLDAASRAQELRGAAGFLPAGRGSRR
                     PRLEAAAAGVPTVDPPGAGAQPELLAAALARLAEDADWRRAQSDAARRSAEAESLAAL
                     ADALEGDVYRAVVARRRRAAPADPLADRPLVTVDLHMHTEHSHDCSVPVADLLDHAEE
                     QGLGAIAITDHNVFSGAQEAAELARGRNLVVIPGEEVKTDSSGEVIGLFLREEIPRGM
                     TMEETIAAIRAQGGIVYLPHPFDRLHAIPDARTLHRHLGEIDVFEVYNARLLLDGYND
                     EALRFARKYNLTMGAGSDAHVLQGVGTGAVRMRAFGNAEEFLISLRSAEILRRPKSLV
                     YLQSMKWVAQARDRRARGGRAAAGARR"
     gene            15351..15935
                     /locus_tag="VFO88_05445"
     CDS             15351..15935
                     /locus_tag="VFO88_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET9545001.1"
                     /translation="MPLAPDDISERYLRKAIAEMNDLGHAIAEAAGTDRVAVLGSGHP
                     LADIFLLKHRPQTAELHEGVAFFGRTGQAILKSLQRLHVDPTAIYGTNCVKYDGQPDE
                     EQRETLRRELHVVQPKLVVVMGQDALSCLNGAEFPLAKRIEERTGELQRLTPTIEALV
                     CPDVDTALDEEKTKRAFWAAFKAIGPWWSEQPPY"
     gene            15911..16627
                     /locus_tag="VFO88_05450"
     CDS             15911..16627
                     /locus_tag="VFO88_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545002.1"
                     /translation="MVRAAALLAAALVLVAYTLVAERLPDVSTWWEVAVVALFVIPLM
                     FVLVWIALPASRLGSLRLGLVALTLVLLAIAASRADLELAANFLKLAAATTLGWWALS
                     FFEEAWWVPLIAVLIVPVDLYSVARGPTREITENRPEVFDALSVFMRVPGESGTANLG
                     LPDVLFFALFLGACVRFGLRAGPTWVAMTLSFGATLALAVALGEPGIAALPLLSLAFV
                     AVNADLLWRGIRDRPASDRA"
     gene            complement(16795..18171)
                     /gene="rlmD"
                     /locus_tag="VFO88_05455"
     CDS             complement(16795..18171)
                     /gene="rlmD"
                     /locus_tag="VFO88_05455"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET9545003.1"
                     /translation="MAAPVSPGEELELRIDSLAYGGNGVARLNGFVVFVRRGLPGDTV
                     TAQVTKVRRSHAEAVVTGILEHGAPRVEAPCVHYPACGGCRFQDLAYEAQVEAKAGQV
                     ADALARIGGLSGFEQEPPVPAESVFHYRNKLEYSFTQTPDGPGLGFHRAGRWDEVLDL
                     ERCWLTSDAGNAIRDAVRTWARAEGLPAYDQEAQSGYLRHLVVREGRYTGQALATLVT
                     APGELVGEERLVAALRRVPEIRSIHWAVNDRPAEVTNLPTKLLWGEPWIEEEILGLRF
                     RIRPNAFFQTNSEMCQVLYRLAGEYAGLTGEETVYDLYCGTGTIGLSLAGSAFAVWGV
                     EASEESVACALENAEVNGITNAAFFAGDVAASLEELRERSGAPDVVVVDPPRAGLSGK
                     ALRRVGELGAARIVYVSCNPTTLAGNAKELSRDFGYRLERVRPVDMFPHTPHVESVAL
                     FTRAGAAA"
     gene            18221..>18664
                     /locus_tag="VFO88_05460"
     CDS             18221..>18664
                     /locus_tag="VFO88_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545004.1"
                     /translation="MPAVLLSLASSLSFGVADFLGGVASRRGAVVTVLLVSHAVGLAG
                     IAVLVAARADGPPDAVFVPLAALSAALGTAGVAALYRGLATGLMSVVAPIAAAAAVIP
                     VVWGAVRGDAPTALQNGGIALALGGVVLVSRTRTRPGAGARLAAGA"
BASE COUNT         2449 a         6881 c         6754 g         2580 t
ORIGIN      
        1 cgaccatcca gtcgacgatc gccttgtcga agttgtcgcc gccgaggtgg ttgtcgccgt
       61 tcgtcgactt cacctcgaag acgccctcgc cgagctcgag gacggagacg tcgaacgtcc
      121 cgccgccgag gtcgaagacg aggatcgtct ggtcggcctc cttgtcgagc ccgtacgcga
      181 gcgaggccgc ggtcggctcg ttgatgatgc ggaggacgtt caggccggcg atcttgcccg
      241 cgtccttcgt cgcctcgcgc tgcgcgttgt tgaagtacgc ggggacggtg acgacggcgt
      301 ccgtgaccgg ctcgccgagg tacgcctccg cgtcggcctt gagcttctgg aggatcatcg
      361 cgctgatctc cggcggcgcg agctccttgt cgcccgcctt cacgcgcacg tcgccgttcg
      421 gcccctgcac caccgagtac gggacgatcg tcatctcctc ggtgacctcg ctgtgcttgc
      481 gccccatgaa gcgcttgatc gaggagacgg tgttctcggg attggtgacc gactgccgct
      541 tcgccggcgc gccgactagc cgctggccgt ccttcgtgaa cgcgacgacc gacggcgtcg
      601 tgcggccgcc ctcggcgttc gggatgacgg tcggctcccc gccctcgagc acggccatgc
      661 agctgttcgt cgtgccgagg tcgattccga tggtcttcgc catgtgttct ctgccccttc
      721 cgttgcgtgt cttcgattcg gtgctcgcta agaaaatcta agcggcttta tagcagatcc
      781 actcgaggtt ggaggggatc cccggggccg ttacacgggc gggcgggcga cgacctggag
      841 cgtctcgagc gtcgggtcgg cggcgtacga caggtgcgtg ccgccggcgg ccgcctcgcg
      901 gagcgctgcg acgatccctg ccgacaccac ctcgccgggc gcgagcgcgg ggatcccggg
      961 cgggtacggg gcgaccgtct cagcggagat gcggccggcg gcacgctcgg cgggcacggt
     1021 ctcgcgcggc gcgtagaacg cctcccgcgg ggtgagggcg acctcgggct cgaccgccca
     1081 gacggcgctc gctgctcccg gatcgcgcgg ctcgccacgg cggcgctcga ccgactcgcg
     1141 cacggcggcg acgaggcgcc cgacgctctc ctccgtgtcg ccgatcgtga ggagcgggac
     1201 gagcgtgtca cggttcgcga gctcgagcgg caagcccgcc gcgacaaggt cgcgctcgac
     1261 ctccagcccg tccgcgccgg tgccggcgag cgcgagcacg agcttcgtcg ggtcgtccgt
     1321 cgcggccacc gtgagcccct cgaccgcctc gagctcctcg cgcgcgtgcc gcgcgagcgc
     1381 gatcgtccgg ccgaggagct cctccccgcg cgcagccatg agcgcccgcg tgcggtcgag
     1441 gctcgcgagg atcgcagcgg acgggctcgt cgtgtggagc atgtcgaacg cgccgtcgag
     1501 ccgcgcgagg tcgagcagcc cgccgcgccc gaagaggtac gcgccctgcg tgaaggcggc
     1561 gagcgtcttg tgggcggacg tcaccatccc gtccgcgccg agcgcgagcg tgtgctgcgg
     1621 gagctccgga tggaagccga ggtgcgcccc ccaggcctgg tcgacgagca gcggggcacc
     1681 gcgcgcgtgc gcggcgtccg cgatcgcggc cacgtcggac aggaccccga cgtacgacgg
     1741 ctcgacgagg aacaccgcgc gcgcgtcccc ggcccgcgcg agcgcgtcct cgacgcgggc
     1801 cgccggcacg gaccgcggca ggccggtcgc ggcgtcgatg tccgggcgca cccagaccgg
     1861 ctcgaggccc gcgaggacga gaccggagaa gagcgaggcg tggaggttcc gcgagacgac
     1921 gattcgcccg ccgtcgccgc cgacggcgag cgcgagcgcc tcgttcccct gcgtcgagcc
     1981 gttcaggcag aagcgggcga ggtcggcgcc ccagaggtcg gccgcgagcg cgtgcgcccg
     2041 ggcgaggacg ccgcgggacg aatggagatc gtcgaccgcg gtcgtgagcg ggaggtcgta
     2101 caggagcgcg ccgtccgcga ggtgcggtga gcgcttgtgg ccgggcgtcg tgaacggagc
     2161 gatccgctcg tcggccaggt acgcgtcggc ggcggttgcg acgggtgtct cccgctgatc
     2221 cacggcgggg ttctaccgga atcggacggg aggacggagg gccgtgcccc ggcgtcgcgc
     2281 gccggggcac ggcgggcagg cgggttcagt cgtccccgcg caccttgtcg acggcgtcgc
     2341 cggccttctc cttggcgcct tccttcgtgt cgccccaggc gccctggacc ttgccctcgc
     2401 cctcgaggcc ctcgtcgccg gtcgccttgc cgacgccttc cttcgtcttg ccctcgacct
     2461 gatcccaatc ggccatttga tgcccccctt gtccgtcgtg gtttctcgcc cactaccccg
     2521 gcccgcggat ccgggaacgc gggctagcgg ggggagagac gtcctcgacc gcgacgccgc
     2581 ggcgaaggag cccgcgtccg tcggcgcggc ccgccggcgc gctgacgatg agggcgacgg
     2641 cgacgacgat cgccgcgccg gcgacgagca cgcgcgccga gatctcctcc ccgacgacgg
     2701 cccagccgag caggaccgcg acgaccgggt tcacgtacgc gtacgtggcg acgagcgaga
     2761 tcggggccgc gcggaggagc cacacgtacg cggtcaggcc gacgagcgag cccatcacga
     2821 tcaggtagac gacgcccgcg agcgactcgc ccgcgatcgc ggccgggtcg aggccggcga
     2881 gctcgccgcc cgccgcgctc gctacgagca ggagcacgcc gcccgcgagc gaggcgaggc
     2941 ccgcggaggc gatcgggtcg ctggggagcc gcgctccgcg cgcgtacagc gacccggcgg
     3001 cccacgagcc ggccgagagg agcgcgacga ggccgcccac cgtgtcgatc ggcccgccgc
     3061 cgaacgggtc gacgagcgcc gcgatcccgg cgaagccgag caggagcccg gcgaccgcgc
     3121 gggccgagag gcgcttgccg aaggcgaccc ggtcgagcac ggcgatccag agcgggatcg
     3181 aggccacgat gagcgcggcc acgccggacg ggatccgcgt ctgcgaccac gcgagcccgc
     3241 cgtggctgcc gaggaagagc gcgccgccga agaccgtcgc ggcgaggatc tgcgcgcgcc
     3301 cgatcggctc gcgcggccgc cgccagagcg cccacgcgaa gagcagcgag cccgccacga
     3361 gatggcgcgc cgacatggcg aggaacggcg ggagcgtccg caccgcgtac tcgatcgcga
     3421 ggaacgtcga cccccagacg acgtagatgg tcccgagcgc cgcgtagacc ttcatcgagc
     3481 gctgaaccgt aaaggccgaa gcggcgcttg acgcgcctgg cccgcgtcca tagcgtcgcc
     3541 gtcgacattc gacacaaggg ggttgacatg agacgggaat ttctcgtcgc gctcgcggtc
     3601 gtcgccgcgg tcgcgctccc ggcgccgggc caggttgcgg ccgcggcgcc gcccgagccc
     3661 gtcaagcagg cgacggcaac cgggacgggc ggcgcggcct cgacggtcga cgccctcgcc
     3721 acggaggcgg cgctcgacat cctcgaccgg ggcgggaacg cggtcgacgc cgccgtcgcc
     3781 gcggccgccg tgctcggcgt cgtcgagccg ttctcctgcg ggatcggcgg cggcggcttc
     3841 atggtcgtct acagcgcgtc cgaccggagc gtcacgacga tcgacggccg cgagacagca
     3901 cccggcgcct acacgcccac gaccctccag gggatcccgt tcgcggaggc ggtcacgagc
     3961 gggctcggcg tcggcgtgcc gggcaccctg cgcacctggc aggccgcgct cgaccgcttc
     4021 gggacgatga agctccagcg gctcctcgcc ccggcgaagt cgatcgcggc gggcggcttc
     4081 ctcgtcgacc agaccttcca cgaccagacc gcggcgaacg ccgcgcgctt cgggaagttc
     4141 acctcgacga gcgagctctt cctcccggac ggcgaggcgc cgccggtcgg cacgcgcttc
     4201 cggaatcccg agctcgccaa gacgtacgcg cagatcggct ccggcccgga ctcgttctac
     4261 cgggggaaga tcgcggcggc gatcgtggac gccgtccgca cgccgccggt ggagcccggc
     4321 gtgacggacg tccgccccgg gctcatgacg ctcgccgacc tcgccgccta tacggcgccg
     4381 ttccgggcgc cgacccgggt cgggtaccgc ggcctcgacg tctacgggat gggcccgccc
     4441 tcgagcggcg gctcgaccgt cggcgaggcg ctgaacatcc tcgagggata cgaccttgcc
     4501 ggcctgagcc gtgaggaggc gctccactac tacctcgagg cgtcggcgct cgcgttcgcc
     4561 gaccggaacc tctacctcgg cgacccggac ttcgtctcga tcccgctcgc cgggcttctc
     4621 tcggacagct acgccgcgga gcgccgtgcg ctcatcggcc cgacgacggg cgtcccgtac
     4681 ccgcccggcg atccgaccga caacgggggg ccgtccacga cgcacctgac cgtctccgac
     4741 cggcacggga acgtcgtctc gtacacgttc acgatcgagc agaccggcgg aagcggaatc
     4801 gtcgtccccg ggtacgggtt cctcctcaac aacgagctga ctgacttcga cttcgtgccc
     4861 ggacgggcga actcgccggc cgcgggcaag cgtccgcgct cgagcatgag cccgacgatc
     4921 gtgctccgtg acgggcggcc gtacgtcgcg gtcgggtctc cgggcggggc gacgatcatc
     4981 acgaccgccc tgcagatcct cgtgaacgag atcgacttcg ggatgacgct ccccgaggcg
     5041 atcgcggcgc cgcgggcgtc gcagcggaac ctggcgaccg tccaggcgga gccggggttc
     5101 atcgtctcgc ccgaggcggc ggcgctcgcg gcccgggggc accgcttcgc ctcgaccccg
     5161 gagatcggag ccgcgaccgg catcgcgttc ctgcccggcg gcttcgtgac cgcggcggcg
     5221 gagccgatcc ggcgcggcgg cgggagcgcg atggtgcagg agccgaccgc cccgatgctc
     5281 gccggcgcgc gcgcgaaggc acagcggggc cgggcgctcc gctaggaggg aacggcacgc
     5341 gtagccgggg cggcgcgatc cgccccggct acgctatgca gatgcagctc gtcgacgggt
     5401 tcggccgccc catccggagc ctgcgcgtgt ccgtcacgga caagtgcaac ttccgctgca
     5461 cctactgcat gcccgcggaa gggctcgagt ggctgcgccg cgacgagatc ctgagcttcg
     5521 aggagatcgg ccggctcgtg cgggtcatgg ccgggatggg cgtggacgag atccggctca
     5581 cgggcggcga gccgctcgtg cgccgcgacc ttccgacgct cgtccgcatg ctcgccgaga
     5641 cgcccggcgt ggacgacctc tcgctcacga cgaacggcgt cctcctcgac cgcctcgccg
     5701 ccccgctcgt cgaggcgggc ctgcggcgcg tgaacgtcag cctcgactcg ctctcgcacg
     5761 tccgcttcgc ggagatcacc cgccgcgacg cgctcgacca ggtgctccgc gggctcgaaa
     5821 cggccgagca gcaccccgag ctgcggccga tcaaggtcaa ctgcgtcgcc gtcaagggct
     5881 tcaccgagga ggaggttccc gcgctcgccg agctggcgcg gcgcaagccg tacgtcgtcc
     5941 gcttcatcga gttcatgccg ctcgacgcgg accgcgcctg ggcgggggac aaggtgctga
     6001 ccggcgacga gatccgcgcc ctgatcgagg agcgctggcc gctcgaggag atccccgcga
     6061 agccgtcgtc gaccgcccgc cgcttccgct tcgcggacgg cgcgggcgag gtcgggttcg
     6121 tgaacccggt ctcggagccg ttctgctcca cgtgcgaccg catccgcctg accgcggacg
     6181 gccacctgcg cacctgcctc ttctcccgcc gggagtggga tctcaagacg cctcttcgcg
     6241 agggcgcgac ggacgaggag ctcgccgccg cgatccgcga ggccgtcgcc cacaaggagc
     6301 tgaagcacag gatcaacgac cccggcttcc tccccgcgag ccggagcatg agccagatcg
     6361 gcggctgagc cgtgcgcgag cggatcgccg ccgtcttccc ccgcggcccg gccgacttcg
     6421 ccctccagct cgggatctgg ttcggcttcc tcgccgcgta ccaggtcgcg cgcggcgtcg
     6481 ccgaccggga cgtcccgcag gcgttcgcga acgggcagtg gatcatcgac ttccagcggg
     6541 cgacctcgac gctcgtcgag ctcgacctgc agcgcgtcgt cctcgaggcg ggcggggtcc
     6601 tcctcgacgt cctcaactgg acgtactggc tctcccagtt cgccgtcctc ggcgtggcgc
     6661 tcctctggat ctacttcttc cgcaccgagg ccttcgcggg tgtccggaac tggatcatcg
     6721 ccgcgaacgt catcggcctg ctcggctacg tcttcctccc gacggcgccg ccgcgcatgt
     6781 tccccggcga gggcttcgtg gacacgctcg cgcactcggc ctcgctgaac cacgggagcg
     6841 ggctcgtcca gctcgcctcg aacccgtacg cggcgatgcc gagcctgcac ggggcggacg
     6901 ccctcatcat cggcctcgcg atggcggcgc tcgtccgctc gtggtggatg aaggcggtct
     6961 ggctcctgtg gcccgcgtgg gtctggttcg cggtcatggc gaccggcaac cacttctggc
     7021 tcgacatcgc ggccgggatc gcggtcgcct gggtcgcgta cgcgctcctc gcgtgggcgg
     7081 gccggcgccg ggacgtgccg cgcttcgccc tctccggctc gtgacgctgt tagcgtcccg
     7141 ccggtgaact cgctcgaacg gctccagggc ggctacatcg gcggcgttcg gcggctcgcc
     7201 gccacggcga tgggccggct ggggcgaacg cgcgtgacgc cgaacatgct cacggtcgcc
     7261 gggatcgcgc tctcgatcgc cggcgccgtc gtcgtctact tcgagtacgc cggctgggga
     7321 ttcttctggc tcggcgcggc gctgttcgtg ctcggcgcga tcctcgacat cctcgacggc
     7381 gcgctcgccc ggagccgggg ggtcgcgtcg gagttcggtg cgttcgtcga ctcgacggtc
     7441 gaccgcgtcg gcgaggcgtt catgctcggc gcgatcgcgc tcgtgctcat gcgggagggc
     7501 tacgaatggg gtgtcgcgct cgcgttcgcg gcgctcgcgg ggtcgttcct cgtgagctac
     7561 gcgcgggcgc gggccgaggc gctcggtctc aagggggacg tcgggttcgg gagccgggcg
     7621 gagcgggtcg tcgtgatcac gaccgggctc gtgctcgcgc cgatccacga gctcgtcctg
     7681 ccggtcacga tggcgatcct cgccgcgacc gcgtgggtga ccgtgctcca gcgggtgctc
     7741 tcggttcgtt cgcagctccg gcaccgctga tagcagtttt gtgatatcac ttcccggcga
     7801 tggccaggga gcgcaccgaa gtcctcgtcc gcatgccggt cgagctgaag cgccgcctcg
     7861 cggacgaggt ccgcgagcgc gggtcgaacc tgaacgacgt cgcggtcgag atcctggcct
     7921 cgcgcttcgg gatcccgttc gacccgaccg gccggaaggg cagcccgggc ggccgaagcg
     7981 gcgacgtcct cctccggatg ccgcgcgccc tcaaggagcg gctgcgcgac cgcgccgagg
     8041 ggcggcgccg caccacgaac gacctcatcg tcgagacgct cgcggagcgg ctcggcctcc
     8101 ccacgaagag aaaggacagc atgagcgaga cgaacggaag ccggaacggc aagcggccgt
     8161 cgggcgagaa ggtccgcgtc gcgttgatcg gcgtcggcaa ctgcgccaac tcgctcctcc
     8221 agggcgtcga gttctacaag gacgcctcgc cggaggagtt cgtgccgggc ctcatgcacg
     8281 tcgacctcgg cggctaccac atccgtgaca tcgagttcac ggccgccttc gacgtgacga
     8341 aggacaaggt cggcaaggac ctcgccgacg cgatctgggc gcatccgaac gacacgtacc
     8401 gcttcgccga ggtcccgaag accgggatca aggtctcgcg cgggatgacg cacgacgggc
     8461 tcggcaagta catctccgag gtggtcgaga aggcgccggg cgacaccgac ccggtggcgg
     8521 agatcctccg ggagacgaag acggacgtgg tggtcaacta cctccccgtc ggctcggagc
     8581 tcgcgacgaa gtggtacacg gagcagatcc tgaacgccgg ctgcgcgatg gtgaactgca
     8641 tgccggtctt catcgcgaag ggcggctact tcgcgaagca gttcgagcag cgcggcctgc
     8701 cgatcatcgg cgacgacatc aagtcccagg tcggcgcgac gatcacgcac cgcgtcctca
     8761 cgagcctctt ccgcgagcgc ggcgtgcacc tcgacaagac gatgcagctg aacgtcggcg
     8821 ggaactcgga cttcctcaac atgctcgagc gcgaccgcct cgagtcgaag aagatctcga
     8881 agacgaacgc cgtcacgtcc atgctcgact acgacatggg cgcggacaac gtccacgtcg
     8941 gcccgtccga ctacgtgccg tggctgaccg accgcaagtg ggcgtacatc cggatggagg
     9001 gctcctcctt cgggaacgtc ccgctgaacg tcgagctcaa gctcgaggtc tgggactcgc
     9061 cgaactcggc cgggatcgtg atcgacgcgg tgcggctcgc gaagctcgcc ctcgaccacg
     9121 gcgtcgccgg cgccctcgag ggcccgagcg cctacctcat gaagtcgccg ccgaagcaga
     9181 tgcccgacga cgacgcgcac gaggcggtcg agagcttcat caggaagtac gcgcgcaagg
     9241 acgagcccgt gcccgcctcc aagtaacact gtgttacaag cgaaggggcc ccgcggggcc
     9301 ccttcgcgtt gcgtcctacc agagggcgaa ggcgcggagc ggcccgccgg agccctgctc
     9361 ccacgggacg acgccgacga cgacggtcgc gccgcgcgcc ggcacctccc cgaggttcgc
     9421 gacgttctcg agcccgtagc ggtcggcgcc caggatggcg aagtgcgcgt cgaaggtcgt
     9481 cgagttgccc gggtcgaggc tgagcgtgtc gaccgcgatc ccggcgacgt cccggttctc
     9541 gatcagccac tcggtggcct cgccggagaa gccggggaag ttgtacggaa acgcgggcgc
     9601 gcccttgaac gcgagcgagt tgttcacctt cgcgtcccag ccggagttca tcgccacgat
     9661 cgccttgcgc gggatccgtc cgcgaccccg ctcgaacctc cgcaggtcgt ccacggtcac
     9721 catcgcgttc gggtcgctcg ccgccttccg ccggatgtcg acgacgacga gcggccgcat
     9781 gagctcgtcc gcctcgatct cgggcgtgag ccgcccgccc gggacgaagt ggcccggcgc
     9841 gtccatgtgc gtgccggagt gctcggtgaa cgtccaccgc tgcgagtaga agccgtccgt
     9901 cgggaagtcg aagaacgtct cgcgcgtcgg ctcgggcccg ccgaagacgg gaaagccggc
     9961 gcggaagagg tgggtgaggt cctgcatgcg cttgaaccgc cgcgagggcc cgccggagcc
    10021 gcgcccgccg ccgaacgccg cgcccgggaa catcgccgcc gccgccgccg cggcgccgcc
    10081 ggcgagcagc gtgccgcggg tgaccgtcgg cgcgccctcc tcctcgatcc gctcacggac
    10141 ggcctcggcc gtgcctggaa gacacatgac ttcctcccct gtcgtgaacg gcccgaggct
    10201 acccgcggcc gcgtgccggt tcaagtacgc ggagagggcc ggcgcgcggg ccggccctct
    10261 cctctccttc cgatcgcctc aggagctact ccacgatctg cgccccgtcg acgcgcggca
    10321 tgctcgagtg gagctcgagc tccgccgcga cgacctggtt gtcccgcggc gggagcggcg
    10381 tgccgatgcg gcagtccgtc tcgtgccgcg ggtcggcgtc ctgcttgccc tggtacgacg
    10441 tctgccccgt gcactggttg tccagcacgc ggaagaggac gtgcgtcgcc gtcgtccccg
    10501 ggaccgccca cgtgcggagg ttcagcgcgg gcgcgaccgg gcgtgggttg tcggccggga
    10561 acgcgtcctc ggccgtcagg aacaggttcc agccgccggc gacggccggg tcgcaggtcg
    10621 ggttcgcgcc cccgcccgcc gtgcaggcgt ggagctcgaa cgaccgcaac gcgacgaacc
    10681 ggttctggcc gggcacgagg agcgcgctcg cccgtgcgag gtcgaacgtc cgctccccgc
    10741 cgagggtgac gagcacccgg cggccctcga cgggcgcgcc ggtcgactgc cacgtcgtcg
    10801 cctcggtgtc gtcgatcagg ttcgcgtggt tcgctccgtc accgctcgcc gtggcgccct
    10861 tcgcctgcga cgcccagttc gtcggcatgt ggatggtgat cgtgcgggac tcgcccgccc
    10921 ggaggttcct cacgtggaag cggacgtgcc cgtaccccgg agcctgagcg acgaagtcgt
    10981 agccctgcgg gctcgggacg aagtcgctcg tcgccccgat cggggtggca cgggcctcgt
    11041 agtggccgac gaacacgcgc gcgttcgcga tcggcgcgcc gccctcgtcc ttcgccacgg
    11101 cctcgaacgt gagcgtcgcc tccccgtgga gcggcgaggc gtagctcgcg accggctccg
    11161 tgtcctgcgt ccccgtcgtg gtcgcgttct ggccgaagcc gcgccgggcg aagccgagcc
    11221 agaggagatc ctggttcgcg cctccgaacc ggagcaggtc ggcggccagg tacgcgtcac
    11281 gggcgtcgag gaacgtcggt gccgtcggca tgaggagcat cgcgtcgaac accgtctgga
    11341 tccagcgccg gttccccggg cactgctcgg gcgggcggtc tccgtcggcg cactcctgct
    11401 ggagccggcc gccgccctgg ccgtagcggt cgacgtagag gtcgcggatg cccttgttcg
    11461 tggcgctcca gatctcgccg tcggcgtgga cctgcggccc gacgatgtcg tagccgaccg
    11521 cgccgaagtt gagcgggttc acccgcacct cgcggccggg cgccgggaac gccccggcgg
    11581 acggccaggc catgttgtag ttccggatgg cgcgcgagtg gttccccgtc gcgtacacgc
    11641 cgacggccca cgcgaggtcg ccgccgggga cgaggttgta ctcgttcagg tgctccatcg
    11701 acacgaagtc gccgaagctc tcgcccatcg cgcccgcgtg gaagcccatg cggttcgagc
    11761 ccttgccgat catccggttc tcgatcatgt ggccgtactc gtggccgatc acggccatgt
    11821 cgtagtcgcc gtccacgcac ggggcgtaga aggcgccggg gatcggctgc cacaggaaca
    11881 tcgccgactg cgacggcgtc ccgtcaggca gcgtccgcat gaacgcgttg tcgcgccccg
    11941 cgtacgtcgg cggcccgtgc gagaccgcgc ccgcctgcac catcccgatg agcgggtcgc
    12001 cctgcgccgt gcccacgccg aagttgtgga gctgggcgtt ccacgccgac tccgtgaagc
    12061 cgagatggta cgaccagttg tgcatgcggc tgtgcatcgc gaagaggttc acggtcgccg
    12121 cgtcgatgtc gttccccgag ccgaccacga ggacgtcggg gtcgcagcgc tcctcgaacc
    12181 agacgttctc ccacgggtag acgtagtcgc gggtcgggct cgtcggccgg tactgcgtcg
    12241 ggcccgggaa gaggaagtcc atccagttct cggcgctccg cgcgtagttg ccggtcgtcg
    12301 tgaacgtcgg cacgcccgcc ggcgggacga cgtcccacgc gaacgccggg tgcggcgacg
    12361 agccggcgag cgcgaggcgg cagccggcct cgttcagcca gcaccacgtc tcgcgcgtgt
    12421 cggcgctcgg gtagttccac gggtagcggt tgagcggggt cgtccgcggc cacgacggga
    12481 agacgctcca cttcgggttg tcgagcgcgt ggtggactgc gctctcgctg aagaggaggc
    12541 ggccggtgtc cgcgtccacg accacgcggt ctccgatcac gtgcccgtcc tcgtccgtgt
    12601 cgaggacgta cgcctcgtac gcggcgatcg tgccgcgcct cggcgtcggg aacgcgacgc
    12661 ggcggacgag ctgcgtgtcc gcgacgcccg cgacgtcgag ctgcgtccag ccggcctgcc
    12721 ggccggcgac gtcgacgtca gagacggacg cgtcgacgcc ggaagagtcg gccgcctcga
    12781 cccaggcctc ggcgaccgag aggtcggccg ccgcgtcgag cgtcgtgtcg ctcgtgagcg
    12841 aggaggacgc gtaggcgacc ctccagcccg cgccggccga gccggtcagc ccgaccgtga
    12901 gcgcgccgtc gggcgccgcg aggagcccgc cgatgcgctg gcggaagacg acggcgtgcg
    12961 cgcctccccc gcccagcggg acggcgctct cgagctcgag cgacgcggtc gagtcgagcc
    13021 ggaagagcgt cgcgttcgcc tcgacccagc gacgcgccgc gctctcggcg tcggcgccgg
    13081 cgaccgcgtc gccgagatgg ccgccgtagc ggatcaccga ctgcggcgtc ccgaacctgt
    13141 tccagcggac ctccgcgtcg agcgcggccg cggcggcgcg ctgcgccgca gtgggcgcga
    13201 gcgtcgccgt ccgcgagtcg aacgtctcgt ggtcgtcccc gtcgagcgac agctggacga
    13261 ccccgagacc ggaagacggg acgagcagcg cgacggctcc caccgttacg gctgcgagca
    13321 gccgtaaccg tccgcctcgt cccttgcaga gcaaggactg cgggtgcatg ttcccctcct
    13381 acgggctccg gagttgacgg gggagatgac gcgtcggagc ccgtgacatc agtaaacgac
    13441 ctcggccgaa agagcaagaa cgggccgaaa cctccacccc gctacactcg cccgcaggcg
    13501 atgggactcc gaatctccat ggtcacgccg ttcgcgtggt cgcagccgca cgccgtgaac
    13561 gagcacgtcg ccggagcggc cgaggagctg cgccgccgcg gccacgaggt ggtcgtcctc
    13621 gccccgtcga accgcgccgc cgacctcgcc gccggccgcc gcgcgctgcg gcgcctcgcg
    13681 cgtgacggcg agccgctccc cgacctcgtc gcgctcggac cggcggtccc ggggccggcg
    13741 cgaacgctcg tcggcgtgcc gctcggcgcg cgcgcgaacc tcgcgctcgc cctctcggcc
    13801 gaccggttcg acgtcgtcca cgcgcacgag cccggcctgc cgagcctctc gtacctggcg
    13861 ctccgccacg cgcgggggct cacggtcgcg acctaccact cgcccgagcg cctcgcctac
    13921 ccgccgggga agcggcagcg cgagaagctc ctggcccgcg tggacgcgct caccgcgacg
    13981 cgcgacgaca cgctcgcgct cgcgcgggac cggtttccgg gcgacttccg ccggctcccg
    14041 ttcggcgtcg acaccgcacg cttccggccg gggcgcgagc ggaagcgctt cgtgctcgag
    14101 tggcgcgcga acgagacggc gcgcgcgacg gccgcgtttc gtgcgctcgc cgacctgccg
    14161 ggctgggagc tcgtcgtcct ccgcacccgt ccgctgagcg gccggccgta cgtcccgcgg
    14221 gccgtgcgcg gccgcgtgca cgtcgcgcgg ggcctcgacg cggcgagccg ggcgcaggag
    14281 ctgcgcggcg ccgccggctt cctgccggcc gggcgcggct cgcgccgccc gcgcctcgag
    14341 gccgccgctg cgggcgtccc cacggtcgac ccgccgggcg ccggggccca gccggagctc
    14401 ctcgccgccg cgctcgcgcg gctcgccgag gacgccgact ggcgccgcgc gcagtcagac
    14461 gcagcgcgcc gctcggccga ggcggagagc ctcgcggcgc tcgcggacgc gctcgagggc
    14521 gacgtctacc gcgccgtcgt cgcccggcgc cgccgcgccg cgcccgccga tccgctcgcc
    14581 gaccggccgc tcgtcacggt cgacctgcac atgcacacgg agcactcgca cgactgctcc
    14641 gtcccggtcg ccgacctcct cgaccacgcg gaggagcagg gcctcggcgc gatcgcgatc
    14701 accgaccaca acgtcttctc gggcgcgcag gaggccgccg agctcgcccg cggccgcaac
    14761 ctcgtcgtca tcccgggcga ggaggtcaag accgacagca gcggcgaggt gatcggcctc
    14821 ttcctgcgcg aggagatccc gcgggggatg acgatggagg agacgatcgc ggcgatccgt
    14881 gcgcagggcg ggatcgtcta cctcccgcat ccgttcgacc ggctccacgc gatccccgac
    14941 gcgcgcacgc tccatcggca tctcggggag atcgacgtct tcgaggtgta caacgcgcgg
    15001 cttctgctcg acggctacaa cgacgaggcg ctccgcttcg cgcggaagta caacctcacg
    15061 atgggcgcgg ggtccgacgc gcacgtgctc cagggcgtcg gcaccggcgc ggtgcggatg
    15121 cgcgcgttcg gaaacgcgga ggagttcctg atctcgctgc gctcggcgga gatcctgcgg
    15181 cggccgaagt cgctcgtgta tctccagagc atgaaatggg tggcgcaggc gcgcgaccgg
    15241 cgggcgcgcg gcggccgggc ggccgcgggc gcgcggcgat gagcgggggc cgcggcgccg
    15301 accccggtag gagagcggcc gggcgagagc gaactacgcc acgaccctcc gtgcctctcg
    15361 ccccggacga catctccgag cgctacctgc ggaaggcgat cgccgagatg aacgatctcg
    15421 ggcatgcgat cgccgaggcc gcgggcaccg atcgtgtcgc cgtcctgggc tccggccacc
    15481 cgctcgcgga catcttcctc ctgaagcacc ggccccagac ggccgagctc cacgagggcg
    15541 tcgcgttctt cgggcggacg ggccaggcga tcctgaaatc gcttcagcgc ctgcacgtcg
    15601 acccgaccgc gatctacggg acgaactgcg tgaagtacga cggccagccg gacgaggagc
    15661 agcgcgagac cctgcgccgc gagctccacg tcgtgcagcc gaagctcgtg gtcgtcatgg
    15721 ggcaagatgc cctctcgtgc ctgaacggag cggagttccc cctcgcgaag cggatcgagg
    15781 agcggacggg cgagctgcag cgcctgacgc cgacgatcga ggcgctcgtc tgcccggacg
    15841 tggacacggc gctcgacgag gagaagacga agcgggcctt ctgggccgcg ttcaaggcga
    15901 tcgggccctg gtggtccgag cagccgccct actagcggcc gcgctcgtcc tcgtcgcgta
    15961 cacgctcgtc gccgagcgtc tgcccgacgt gagcacgtgg tgggaggtgg ccgtcgtcgc
    16021 cctcttcgtg atcccgctca tgttcgtgct cgtctggatc gccctgccgg ccagccgtct
    16081 cgggtcgctg cggctcggcc tcgtcgcgct gacgctcgtc ctgctcgcga tcgcggcctc
    16141 gcgggccgac ctggagctgg cggccaactt cctgaagctc gcggcggcga cgacgctcgg
    16201 ctggtgggcg ctctccttct tcgaggaggc gtggtgggtg ccgctgatcg ccgtgctgat
    16261 cgtcccggtc gacctctact cggtcgcgcg gggcccgacg cgcgagatca ccgagaaccg
    16321 gccggaggtc ttcgacgcgc tctcggtgtt catgcgcgtc cccggcgaga gcgggacggc
    16381 gaacctcggc ctcccggacg tcctcttctt cgcgctcttc ctcggggcgt gcgtgcgctt
    16441 cgggctgcgc gcgggcccca cgtgggtggc gatgacgctg tccttcggcg cgacgctcgc
    16501 gctcgcggtc gccctcggcg agccggggat cgcggcgctg ccgctcctct cactcgcgtt
    16561 cgtcgccgtg aacgcggacc tgctctggcg gggcatccgc gaccggccgg cgagcgatcg
    16621 cgcatgagcc ggcacggcgc cgatgtggac gggtcgtcac gaactcgtga cggccgcgtg
    16681 acggttccgc gcgcccgcgc tcgatagcgt tcgtgctggg agcctcgcgc ccccgttggg
    16741 ggggtgcgct ctcgcccggc aggagccacg gctgcggtgg acagggggcc cggctcaggc
    16801 ggcggcgccg gcgcgcgtga agagcgcgac cgactcgacg tgcggcgtgt gcgggaacat
    16861 gtccaccggc cgcacccgct ccagccggta gccgaagtcg cgcgagagct ccttcgcgtt
    16921 cccggcgagc gtcgtcgggt tgcacgagac gtacacgatc cgcgcggcgc cgagctcgcc
    16981 cacgcggcgg agcgcctttc ccgagagccc tgcccgcggc gggtcgacga ccacgacgtc
    17041 cggcgcgccg gaccgctcgc ggagctcctc gagcgacgcg gcgacgtcgc ccgcgaagaa
    17101 cgccgcgttc gtgatcccgt tcacctccgc gttctcgagc gcgcacgcga cggactcctc
    17161 ggacgcctcc acgccccaca ccgcgaacgc cgacccggcg agcgagagcc cgatcgtccc
    17221 cgtcccgcag tagaggtcgt acaccgtctc ctcgccggtc agccccgcgt actcgccggc
    17281 gagccggtag agcacctggc acatctccga gttcgtctgg aagaacgcgt tcgggcggat
    17341 ccggaaccgc agcccgagga tctcctcctc gatccacggc tccccccaga ggagcttcgt
    17401 cgggaggttc gtcacctccg ccggccggtc gttcacggcc cagtggatcg agcggatctc
    17461 cggcacgcgg cgcagtgccg cgacgagccg ctcctcgccg acgagctcgc cgggcgcggt
    17521 cacgagcgtc gcgagcgcct ggcccgtgta ccgcccctcg cggacgacga ggtggcgcag
    17581 gtacccgctc tgcgcctcct ggtcgtacgc gggcaggccc tcggcgcggg cccaggtccg
    17641 cacggcgtcc cggatcgcgt tcccggcgtc cgaggtgagc cagcagcgct cgagatcgag
    17701 cacctcgtcc cagcgccccg cccggtggaa cccgagcccc ggcccgtcgg gcgtctgcgt
    17761 gaaggagtac tcgagcttgt tccggtagtg gaagaccgac tcggccggga ccggcggctc
    17821 ctgctcgaag ccgctgagcc cgccgatccg cgcgagcgcg tccgcgacct ggccggcctt
    17881 cgcctcgacc tgcgcctcgt acgcgagatc ctggaaccgg cagccgccgc aggcgggata
    17941 gtgaacgcac ggcgcctcga cccgcggcgc gccgtgctcg aggatcccgg tcacgaccgc
    18001 ctcggcgtgc gagcggcgca ccttcgtcac ctgcgccgtg accgtgtcgc cggggagccc
    18061 gcgccggacg aagacgacga agccgttcag gcgcgcgacg ccgttcccgc cgtaggcgag
    18121 cgagtcgatg cggagctcga gctcctcccc gggcgagacg ggcgcagcca ccgtcgcagt
    18181 ctaggcgtga cgcgccgtcc ctctgcgagg cttcgcggcc gtgcccgcgg tcctgctgtc
    18241 gctcgcctcc agcctctcgt tcggcgtcgc ggacttcctc ggcggcgtcg cctcgcgccg
    18301 cggggcggtc gtgaccgtgc tcctcgtgtc gcacgcggtc gggctcgcgg ggatcgccgt
    18361 gctcgtcgcc gcacgggcgg acgggccgcc ggacgcggtc ttcgtgccgc tcgccgcgct
    18421 ctctgccgcg ctcgggacgg ccggcgtcgc ggcgctctac cgcggcctcg ccaccgggct
    18481 catgagcgtt gtcgcgccga tcgcggcggc ggcggcggtg attcccgtcg tctggggcgc
    18541 ggtgcgcggc gacgcgccga cggcgctcca gaacggcggg atcgcgctcg cgctcggcgg
    18601 cgtcgtcctc gtctcgcgga cgcgcacgcg ccccggcgcc ggcgcccggc tcgcggcggg
    18661 ggcg
//
LOCUS       DASTFC010000180         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_926437,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000180 DASTFC010000000
VERSION     DASTFC010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926437"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFO88_05465"
     CDS             <1..787
                     /locus_tag="VFO88_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HET9545005.1"
                     /translation="RMVEAAGADIVDINFGCPVGKVTKTGAGATLLEDPERAAAIVGA
                     VAEAVDTPVTVKMRRGLRNGSRAALELGPRLAEAGAAALTLHPRSAKQMYTGTADHGL
                     TAELVERVDVPVIASGDITSRGRALEVLGQTGAAAVMVGRAAQGDPWTVGEIAGLVHE
                     RPSREEVVAELLLFMHDTVEEMGEERATGFLKKFYGWYLGRGRFPKPFKQELVRLATL
                     AEVEDRLLAAAPGAAGHLERLRAETPSLEHTTLDLPVSIYAGG"
     gene            complement(830..1696)
                     /locus_tag="VFO88_05470"
     CDS             complement(830..1696)
                     /locus_tag="VFO88_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545006.1"
                     /translation="MLLGQANESWGDELRNGFEGFFEFIPNLIGFLVVLIVGFFVAKL
                     LANVVTRLLGRSRLDDRLTTGQGGTYVSKVTERPSWLLGRVVFWALFLGVLAIAIPIL
                     GIAAFTDFVGAIFAYLPNVLAALLIFLVASAIAAAAGALVARTMGDTPTGRVVGTVVP
                     VLIMAVAVFMILDQLMIAETIVTITYAALMGAIALGAALAFGLGGREVAARMLEGAYV
                     KGQASTEQVRRDLQVGRERAREDVDRTRERIEGDDVAGPPSRVGTASEYEETQVGTVG
                     TRQRPDDPMSSP"
     gene            1804..>4791
                     /locus_tag="VFO88_05475"
     CDS             1804..>4791
                     /locus_tag="VFO88_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9545007.1"
                     /translation="MATTITGERRIVSVLIADVVNSTGIGERLGPERSKFLMDEVQRI
                     MTAQIRRYDGTVVQRVGDEIFAVFGAPVAHEDDGERAVRAALSLQRAIARYAEEVRDA
                     YEVELAVRVGVHTGPVVVPGDDGKDIGERWNALGDTVNVASRLQELAAPGEVAMGAET
                     ARQVEGCFEVEELGEHELQGKSAPFATFKVRGVRDPERPAPEGPLVGRDFELTVLERA
                     MDGLREGKGVIVSVIGEAGIGKSRLAAEVRAEYGDAIRFVEGRAVSYAQSFPYSPVRD
                     LLRNWLGVGASTPETRVRLELKAQVAGLFGEHADEAYPFLANLLGLTLEPDAAERVRE
                     LNRESVQHQTFEVFSELVCRLSSELPLCLVLEDLHWADEATLELLEETLRVTEEAAVG
                     FLLLHRTEREHRSWHLGERARQLYPHRYREIELRPLPGDASRLLVEEAADGELPPSVE
                     ELLVERAGGNPFFLQEAFRDLVERGALQRSNGSWRLAVDADELAVPALVQGALQARLD
                     RLDPGTREVLSIAAVAGRTFGLPLLETLVPPERVRLALSELQRLDLIVEVRRRPTPEY
                     RFRHGLVQEVSYSTLVEPTRRELHRRVGEALEGLYDESREEIFDLLARHFTEADEPEK
                     AVEYLLAAGDKARQLYADQEALEHYRRARSFLARMRDERRARDTLFKMALAHHLAFDF
                     EQAEEAYDEAFCCRVDPAPRPQPTERITTAALAPDDVTPGESYTSEGAHFISHLFQGL
                     LVVDRELNVVPAMADNMRVSSDGLTYLFRLREDAKWSDGEPVTADDFLYAWERVREEQ
                     PITVFLMEDIEGYQALDDRTLEVRLREPRSYFPYILASNWSFPRPKHLCERLGEDWSK
                     PENLVTNGPYTLEQLSDEGALLVANPHWAGPRGNVREIEVRFAGESTTLVEEWISGRY
                     DVLQAHDPETRDAPETVGDLIPELSLTYVGFRPDRGPFANELVRKAFAHAIDREAVRP
                     GRFGLLTAATRGGAIPPAMPGHSH"
BASE COUNT          673 a         1755 c         1735 g          628 t
ORIGIN      
        1 acggatggtc gaggcggcgg gcgcggacat cgtggacatc aacttcggct gcccggtcgg
       61 gaaggtgacg aagacgggcg ccggcgcgac gctcctcgag gacccggagc gcgcggccgc
      121 gatcgtcggc gcggtggccg aggccgtcga cacgccggtg accgtgaaga tgcgccgcgg
      181 gctccggaac ggctcgcgcg ccgcgctcga gctcggccct cggctcgccg aggcgggcgc
      241 cgccgcgctc acgctccacc cgcgctcggc gaagcagatg tacaccggca ccgccgacca
      301 cgggctcacg gcggagctcg tcgagcgcgt cgacgtcccg gtgatcgcgt ccggcgacat
      361 cacgtcgcgc ggccgcgcgc tcgaggtgct ggggcagacc ggagccgccg ccgtcatggt
      421 cgggcgcgcc gcccagggcg acccgtggac ggtgggggag atcgccgggc tcgtgcacga
      481 gcggccgtcg cgcgaggagg tggtggccga gctcctcctc ttcatgcacg acacggtcga
      541 ggagatgggc gaggagcgcg cgaccgggtt cctgaagaag ttctacggct ggtacctcgg
      601 gcgcgggcgc tttccgaagc cgttcaagca ggagctcgtc cggctcgcga cgctggccga
      661 ggtggaggac cggctcctcg cggccgcgcc cggggccgcg gggcacctcg agcgcctgcg
      721 cgccgagacg ccctcgctcg agcacacgac gctcgacctg cccgtctcga tctacgcggg
      781 cggataacgg cgggagcggc ccgaaggccg cccccgccgc ggttcgcgct tacgggctcg
      841 acatgggatc gtccggacgc tgccgcgtcc cgaccgtccc gacctgcgtc tcctcgtact
      901 cggacgccgt cccgacgcgg gacggcggcc cggcgacgtc gtcgccctcg atccgctcgc
      961 gcgtgcggtc gacgtcctcc cgcgcccgct cgcggccgac ctgcaggtcg cggcggacct
     1021 gctccgtcga ggcctggccc ttgacgtacg cgccctcgag catccgcgcg gcgacctccc
     1081 ggccgccgag cccgaacgcg agcgccgcgc cgagcgcgat cgcgcccatg agcgccgcgt
     1141 acgtgatcgt cacgatcgtc tcggcgatca tgagctggtc gaggatcatg aacacggcga
     1201 ccgccatgat caggaccggc acgacggtcc cgacgacacg gccggtcggc gtgtcgccca
     1261 tcgtcctggc cacgagcgct ccagccgccg ccgcgatcgc gctcgcgacg aggaagatga
     1321 ggagcgccgc gaggacgttc ggcaggtacg cgaagatcgc ccccacgaag tccgtgaacg
     1381 ccgcgatgcc gaggatcggg atcgcgatcg cgaggacgcc caggaagagc gcccagaaca
     1441 cgacccgccc gagcagccac gacggccgtt cggtcacctt cgagacgtac gtcccgccct
     1501 gccccgtcgt cagccggtcg tcgagcctgc tacgcccgag cagccgcgtg acgacgttcg
     1561 ccagcagctt cgcgacgaag aagccgacga tgaggacgac gagaaacccg atcaggttcg
     1621 ggatgaactc gaagaatccc tcgaacccgt tacggagctc gtctccccag ctctcgtttg
     1681 cctgtcctag cagcatgttc cccccgtcgt ttctttgagt tgtggaacga gctagcggga
     1741 tccccgccgc cggagcgccc aaaacgcggg gcggcgagcg cccgccccgc tataactcgt
     1801 ctcatggcca cgacgatcac cggtgagcgc cggatcgtct cggtcctgat cgcggacgtc
     1861 gtcaactcga cggggatcgg cgagcggctg gggcccgagc gctcgaagtt cctcatggac
     1921 gaggtccagc ggatcatgac cgcccagatc cggcggtacg acggcacggt cgtgcagcgc
     1981 gtcggcgacg agatcttcgc cgtcttcggc gcgccggtcg cgcacgagga cgacggcgag
     2041 cgcgccgtcc gggccgcgct ctcgctccag cgggcgatcg cgcgctacgc ggaggaggtg
     2101 cgcgacgcct acgaggtcga gctcgcggtt cgcgtcggcg tccacacggg gccggtcgtc
     2161 gtcccgggcg acgacggcaa ggacatcggg gagcgctgga acgcgctcgg cgacacggtg
     2221 aacgtcgcgt cgcgcctgca ggagctcgcc gcaccgggcg aggtggcgat gggcgccgag
     2281 acggcgcgcc aggtggaggg ctgcttcgag gtcgaggagc tcggcgagca cgagctccag
     2341 ggcaagagcg cgccgttcgc gacgttcaag gtgcgcggcg tccgcgaccc cgagcgcccc
     2401 gcgccggagg gcccgctcgt cgggcgcgac ttcgagctga cggtgctcga gcgcgcgatg
     2461 gacggcctgc gcgagggcaa gggcgtgatc gtctccgtga tcggcgaggc cgggatcggc
     2521 aagtcgcggc tcgcggccga ggtgcgcgcg gagtacggcg acgcgatccg cttcgtcgag
     2581 gggcgcgcgg tctcgtacgc gcagagcttc ccgtactcgc ccgtccgcga cctgctccgc
     2641 aactggctcg gcgtcggcgc ctcgacgccc gagacgcgcg tgcggctcga gctcaaggcg
     2701 caggtagccg gcctcttcgg cgagcacgcg gacgaggcct acccgttcct cgcgaacctc
     2761 ctcggcctga cgctcgagcc cgatgcggcc gagcgcgtcc gcgagctgaa ccgcgagagc
     2821 gtgcagcacc agacgttcga ggtcttctcc gagctcgtct gccggctctc gagcgagctc
     2881 ccgctctgcc tcgtcctcga ggatctccac tgggcggacg aggcgacgct cgagctgctc
     2941 gaggagacgc tgcgcgtcac cgaggaggcg gcggtcgggt tcctgctcct ccaccgcacc
     3001 gagcgggagc accgctcctg gcacctcggc gagcgcgcgc ggcagctcta cccgcaccgc
     3061 taccgcgaga tcgagctgcg cccgctgccc ggcgacgcga gccggctgct cgtggaggag
     3121 gcggcggacg gcgagctgcc gccctcggtc gaggagctgc tcgtcgagcg ggccggcggc
     3181 aacccgttct tcctccagga ggccttccgc gacctcgtcg agcggggcgc gctgcagcgc
     3241 tcgaacggct cgtggaggct cgccgtcgac gcagacgagc tcgccgtgcc cgcgctcgtg
     3301 cagggcgcgc tccaggcgcg gctcgaccgg ctcgaccccg gcacgcgcga ggtgctctcg
     3361 atcgccgcgg tcgccgggcg gacgttcggg cttccgctcc tcgagacgct cgtcccgccg
     3421 gagcgcgtgc gcctcgccct gagcgagctg cagcggctcg acctgatcgt cgaggtgcgg
     3481 cggcgtccga cccccgagta ccgcttccgc cacgggctcg tccaggaggt ctcgtactcg
     3541 acgctcgtcg agccgacgcg ccgcgagctg caccgccgcg tcggcgaggc gctcgagggg
     3601 ctctacgacg agtcccgtga ggagatcttc gacctgctcg cgcgccactt cacggaggcc
     3661 gacgagccgg agaaggcggt cgagtacctc ctggccgccg gggacaaggc gcgccagctc
     3721 tacgccgacc aggaggcgct cgagcactac cggagggcgc gctccttcct cgcccggatg
     3781 cgcgacgagc ggcgggcccg cgacacgctc ttcaagatgg ccctcgcgca ccacctcgcg
     3841 ttcgacttcg agcaggcgga ggaggcctac gacgaggcgt tctgctgccg cgtcgacccc
     3901 gcgccgcgcc cgcagccgac cgagcggatc acgaccgccg cgctcgcccc ggacgacgtg
     3961 acgcccggcg agtcgtacac gagcgagggt gcgcacttca tctcgcacct cttccagggg
     4021 cttctggtcg tcgaccggga gttgaacgtc gtcccggcga tggccgacaa catgcgcgtc
     4081 tcgagcgacg gcctcacgta cctcttccgg ctgcgcgagg acgcgaagtg gagcgacggc
     4141 gagccggtga cggcggacga cttcctctac gcgtgggagc gggtgcgcga ggagcagccg
     4201 atcaccgtgt tcctcatgga ggacatcgag ggctaccagg cgctcgacga ccgcacgctc
     4261 gaggtgcggc tgcgcgagcc gcgcagctac ttcccgtaca tcctcgcctc gaactggtcg
     4321 ttcccgcgcc cgaagcacct gtgcgagcgg ctcggcgagg actggtcgaa gcccgagaac
     4381 ctcgtcacga acgggccgta cacgctggag cagctctccg acgagggcgc cctcctcgtc
     4441 gcgaacccgc actgggcggg cccgcgcggc aacgtccgcg agatcgaggt gcgcttcgcg
     4501 ggcgagtcga cgacgctcgt cgaggagtgg atctcggggc gctacgacgt cctccaggcc
     4561 cacgacccgg agacgcgcga cgcgccggaa acggtcggcg acctgatccc cgagctctcg
     4621 ctcacgtacg tcggcttccg gcccgaccgc gggccgttcg cgaacgagct cgtccgcaag
     4681 gcgttcgcgc acgcgatcga ccgcgaggcc gtgcggcccg ggcggttcgg gctcctcacg
     4741 gcggcgacgc ggggcggcgc gatcccgccg gcgatgcccg gccactcgca c
//
LOCUS       DASTFC010000181         4623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_778209,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000181 DASTFC010000000
VERSION     DASTFC010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778209"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFO88_05480"
     CDS             <1..499
                     /locus_tag="VFO88_05480"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET9545008.1"
                     /translation="EAQSQQHVPPGDIDLEEFEYNTYPPSSGPHNPQPAIYGEYQSPV
                     PTDRYLHSQEHGGITIQYGAEVPEDVVQRLVNWFRSDPRGLILAPLADEPEAEDLQDR
                     IVLTAWIAEREVEDDPRSEITKQEGKLAVCDTFDEGAFDDFKDDHIARGPELFALDDL
                     MPGQG"
     gene            510..590
                     /locus_tag="VFO88_05485"
     tRNA            510..590
                     /locus_tag="VFO88_05485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:543..545,aa:Leu,seq:taa)
     gene            629..967
                     /locus_tag="VFO88_05490"
     CDS             629..967
                     /locus_tag="VFO88_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9545009.1"
                     /translation="MILIGAILLAIYVLPSPWGVIGVIVAAIVEAGETGFWFWLSRRR
                     KVQAGAETLIGASGVVTSACRPEGLVRVQGELWAAYCQYGADPGDEVRVVSRDGLVLT
                     VERGRAEAPA"
     gene            978..2270
                     /locus_tag="VFO88_05495"
     CDS             978..2270
                     /locus_tag="VFO88_05495"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET9545010.1"
                     /translation="MSSEAATAVRGDLPPELDDPLFRTALGQLDGALERAGVSDSVAE
                     VLRFPERALSVAIPVRLDDGTLSVFPGYRVQHSSVLGPTKGGVRYDPAVTLGECAALA
                     MWMTWKCALLGLPYGGAKGGVRCDPRSLSLAELERLTRRFTSELLPIIGPQRDIPAPD
                     MATDAQTMAWMMDTYSMQVGHAVPEIVTGKPLSVGGSVLRPEATGIGVVMVVEGAVER
                     LGWTLEGARCVVQGYGKVGAIAAAELAARGARVTAVSDIEGGIHAEGGLDLAALDAWL
                     RETGGIAGFAGSRAVTNAELLELDCDVLVLAATERQVTETNAGLISARLVAEGANGPT
                     TLEADEILFARGIPVLPDILTNAGGVAVSYFEWVQDLQRLFWGPDEIRTRLGDLMRDA
                     LERVWALAEAEELTLRSAALVQSIRHVAAALQSRGVYP"
     gene            2267..2674
                     /locus_tag="VFO88_05500"
     CDS             2267..2674
                     /locus_tag="VFO88_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9545011.1"
                     /translation="MKVREALVADPRVVAPSASAREVAELLLRPHVESALVADEGRLV
                     GCVTRESLVAAVARGVDLAGATAGDLADGDVTTVGPDDALDDTLHLMVERDLDRVVVV
                     EDGHLLGILAREPILRRLAEDEPPADEPEGEPG"
     gene            complement(2671..3555)
                     /locus_tag="VFO88_05505"
     CDS             complement(2671..3555)
                     /locus_tag="VFO88_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9545012.1"
                     /translation="MGQAGTATYPGGVGRLAARLPAEGFFVGSAVFHYLGPAFAVLLF
                     ARIEPLGVAWLRILTAAVLFALWRRPWRALGTLDAPGRRTVVAMGLVLAAMNPCFYVA
                     IDRLPLGTVAAIEFLPVIALAALGARTRRNGLALLAASAGVYLLTDVRLGGDPLGVAF
                     AVANAGLFALYIVLAHRLANARGIGGIDGLAGAMLVAALAALPIGAWDAAPAFVDPVA
                     LGAAVGVGVTSSVIPYVCDQLAMARMARATYALLVSLLPATATVIGLVVLAQVPSPVE
                     AAGVALVVCGVALRREPG"
     gene            3678..4121
                     /locus_tag="VFO88_05510"
     CDS             3678..4121
                     /locus_tag="VFO88_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET9545013.1"
                     /translation="MNVALLQELQADARLSIAELGRRVNLSAPAVAERLQRLERAGVI
                     TGYRAVVDPKALGYPIAAFVRIRPTTRRLQQIPQLAREIPEIVECHRVTGEDCYVLKL
                     HLRSMDDLEDILDRLIVLGQTTTSIIHSSPLDGRPLPLDTTQQQV"
     gene            complement(4106..>4623)
                     /locus_tag="VFO88_05515"
     CDS             complement(4106..>4623)
                     /locus_tag="VFO88_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545014.1"
                     /translation="GVSWRASLDIGCVRLTERFLAGDPPSADELAACRGAVRALLAEC
                     VPEDVRPSAAIGVAGTVTTLATLHLGLPEEDPERLHGHRVPAAWVEEELARLASATVA
                     ELRARPGIHPDRAPVMVAGAVVVADVLGHFGLAELEVSEQDILHAGALAAAELPAPDE
                     DGVPPGAYTCC"
BASE COUNT          641 a         1677 c         1735 g          570 t
ORIGIN      
        1 cgaggcgcag agccagcagc acgtcccgcc cggcgacatc gacctcgagg agttcgagta
       61 caacacgtat ccgccctcgt ccggcccgca caacccgcag ccggccatct acggcgagta
      121 tcagagcccc gtcccgaccg atcgctacct ccacagccag gagcacggcg gcatcacgat
      181 ccagtacggc gccgaggtgc ccgaggacgt cgtccagcgg ctcgtgaact ggttccgctc
      241 cgacccgcgc gggctgatcc tcgcgccgct cgccgacgag cccgaggcgg aggacctgca
      301 ggacaggatc gtcctgacgg cctggatcgc cgagcgggag gtcgaggacg acccgcgctc
      361 cgagatcacg aagcaggagg gcaagctcgc cgtctgcgac acgttcgacg agggcgcgtt
      421 cgacgatttc aaggacgacc acatcgcccg cgggcccgag ctcttcgcgc tcgacgacct
      481 catgcccggc cagggctaac cttcttcccg ccggggtggc ggaacggtag acgcggggga
      541 cttaaaatcc tctgccttcg ggcgtgtggg ttcgaatccc acccccggca tccacgggtc
      601 gggcgcggcg acggactagc cttcgtccgt gatcctgatc ggggcgatcc tcctcgcgat
      661 ctacgtgctg ccatcgccgt ggggcgtgat cggggtgatc gtggcggcga tcgttgaggc
      721 gggggagacc ggcttctggt tctggctctc gaggcggcgg aaggtgcagg ccggggccga
      781 gacgctcatc ggggcgagcg gcgtcgtcac atccgcgtgc cggccggagg ggctcgtgcg
      841 cgtccagggc gagctctggg cggcctactg ccagtacggc gccgatccgg gcgacgaggt
      901 gcgggtcgtc tcccgggacg ggctcgtgct cacggtcgag cgcgggcggg ccgaggcgcc
      961 tgcatagact ggccgccgtg tcgagcgagg cagcgacagc cgtccggggc gacctgccgc
     1021 ccgagctcga cgatccgctg tttcgcaccg cgctcgggca gctcgacggc gcgctcgagc
     1081 gcgccggcgt gagcgactcg gtcgccgagg tgctccggtt ccccgagcgg gcgctctccg
     1141 tggcgatccc cgtccggctc gacgacggga cgctctccgt cttccccggc taccgcgtgc
     1201 agcactcgag cgtgctcggg ccgacgaagg gcggcgtccg ctacgacccc gcggtcacgc
     1261 tcggggagtg cgcggcgctc gccatgtgga tgacctggaa gtgcgcgctc ctcggcctcc
     1321 cgtacggggg cgcgaagggc ggcgtgcgct gcgacccgcg gtcgctctcg ctcgccgagc
     1381 tggagcggct cacgcggcgg ttcacgtcgg agctgcttcc gatcatcggc ccgcagcgcg
     1441 acatccccgc gcccgacatg gcgaccgacg cgcagacgat ggcgtggatg atggacacgt
     1501 actcgatgca ggtgggccac gcggtgccgg agatcgtgac gggcaagccg ctctccgtcg
     1561 gcggctccgt cctgcggccc gaggcgacgg ggatcggcgt cgtcatggtc gtcgagggcg
     1621 cggtcgagcg gctcggctgg acgctcgagg gagcgcgctg cgtcgtccag ggctacggga
     1681 aggtgggcgc gatcgcggcc gcggagctcg ccgcgcgcgg cgcgcgcgtc acggcggtct
     1741 cggacatcga gggcggcatc cacgccgagg gcgggctcga cctggccgcg ctcgacgcat
     1801 ggctgcgcga gacgggcggg atcgccgggt ttgccggctc cagggcggtc acgaacgcgg
     1861 agctgctcga gctcgactgc gacgtcctcg tcctcgccgc gaccgagcgg caggtgacgg
     1921 agacgaacgc gggcctcatc tccgcgcggc tcgtcgcgga gggcgcgaac gggccgacga
     1981 cgctcgaggc ggacgagatc ctcttcgcgc gcgggatccc ggtgctcccc gacatcctca
     2041 cgaacgcggg cggcgtcgcc gtctcgtact tcgagtgggt gcaggatctc cagcggctct
     2101 tctgggggcc ggacgagatc cggacgcggc tcggcgacct catgcgggat gcgctcgagc
     2161 gcgtctgggc gctcgcggag gcggaggagc tgaccctccg gagcgcggcg ctcgtgcaga
     2221 gcatccggca cgtggccgcg gcgctccagt cgcgcggggt ctatccgtga aggtacggga
     2281 ggcgctcgtc gcggatccgc gtgtcgtcgc gccgtcggcg agcgcacgcg aggtggcgga
     2341 gctgctcctg cgcccgcacg tcgagagcgc gctcgtcgcg gacgagggca ggctcgtcgg
     2401 ctgcgtgacg cgcgagtcgc tcgtcgcggc ggtggcgcgc ggcgtcgatc tcgccggcgc
     2461 gacggcgggc gacctagccg acggggacgt gaccacggtc ggccctgacg acgccctcga
     2521 cgacacgctg cacctgatgg tcgagcgcga cctcgaccgg gtcgtcgtcg tggaggacgg
     2581 gcacctgctc gggatcctcg cgcgcgagcc gatcctccgc cgtctggccg aggacgagcc
     2641 gcctgcggac gagccggagg gcgagcccgg ctagcccggc tcccgccgca gcgcgacccc
     2701 gcacacgacg agcgcgaccc ccgccgcctc gaccggcgac ggcacctgcg ccagcaccac
     2761 gagcccgatc acggtcgcgg tcgcggggag gagggagacg agcagcgcgt aggtcgcccg
     2821 ggccatgcgg gccatcgcga gctgatcgca gacgtagggg atgacggacg acgtgacgcc
     2881 gaccccgacc gccgcgccga gggcgaccgg gtcgacgaag gccggcgcgg cgtcccaggc
     2941 gccgatcggg agcgccgcga gcgcggcgac gagcatcgcc ccggcgaggc cgtcgatccc
     3001 gccgatcccg cgcgcgttcg ccaggcggtg agcgaggacg atgtagagcg cgaagagccc
     3061 cgcgttcgcg accgcgaacg cgacgcccag cgggtctccg ccgagtcgca cgtcggtcag
     3121 gaggtagacg cccgcggacg cggcgaggag cgcgaggccg ttcctgcgcg tgcgcgcgcc
     3181 gagcgccgcc agcgcgatca ccggcaggaa ctcgatcgcg gccaccgtcc cgagcggcag
     3241 ccggtcgatc gcgacgtaga agcacgggtt catcgcggcg aggacgaggc ccatcgcgac
     3301 gaccgtgcgc cgtccgggcg catcgagcgt cccgagcgcg cgccagggcc gccgccagag
     3361 cgcgaagagg acggcggcgg tgaggatccg gagccacgcg accccgagcg gctcgatccg
     3421 ggcgaagagc agcaccgcga aggccgggcc gaggtagtgg aagaccgcgc tgccgacgaa
     3481 gaagccctcc gcgggcagcc gcgccgcgag acgcccgacg ccgcccggat acgttgccgt
     3541 tcccgcctgt cccatgccga aagtcttccg gcctggctcc cgggctgcca tacttcatcg
     3601 aagtggtttc ccgccagata cctacggatc gaacgcatcc agcccggcgc accggcgaac
     3661 cgcaggcggt cgacgcgttg aacgttgcgc tcctgcagga gctacaggcg gacgcacggc
     3721 tctcgatcgc cgagctcggc cgccgcgtga acctctccgc gccggccgtc gccgagcggc
     3781 tccagcggct cgagcgggcc ggcgtgatca cgggctaccg cgcggtcgtc gacccgaagg
     3841 cgctcggcta tccgatcgcc gccttcgtcc ggatccggcc gaccacccgc cggctgcagc
     3901 agatcccgca gctcgcgcgc gagatcccgg agatcgtcga gtgccaccgc gtgacgggcg
     3961 aggactgcta cgtgctgaag ctccacctgc gctcgatgga cgacctcgag gacatcctcg
     4021 accggctgat cgtgctcggc cagacgacga cgtcgatcat ccactcgtcg ccgctcgacg
     4081 ggcggccgct gccgctcgac acgactcagc agcaggtgta ggcgccgggc gggacgccgt
     4141 cctcgtcggg cgccggcagc tcggcggcgg cgagcgcgcc cgcgtggagg atgtcctgct
     4201 cgctcacctc gagctccgcg agcccgaagt gcccgaggac gtcggcgacg acgaccgcgc
     4261 cggcgaccat gaccggcgcg cgatcggggt ggatccccgg gcgggcgcgc agctccgcga
     4321 ccgtggcgga cgcgaggcgg gcgagctcct cctccaccca ggccgcgggg acgcggtgcc
     4381 cgtggagtcg ctccggatcc tcctccggca gccccaggtg cagggtcgcg agggtcgtca
     4441 ccgtcccggc cacgccgatc gcggccgacg ggcgcacgtc ctcgggaacg cactcggcga
     4501 gcagcgcccg caccgcgccg cggcaggccg cgagctcgtc cgccgacggc ggatcgcccg
     4561 cgaggaagcg ctccgtcagc cggacgcacc cgatgtcgag gctcgcccgc cacgagacgc
     4621 cgt
//
LOCUS       DASTFC010000182         4918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_909952,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000182 DASTFC010000000
VERSION     DASTFC010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909952"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFO88_05520"
     CDS             complement(<1..859)
                     /locus_tag="VFO88_05520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET9545015.1"
                     /translation="MTATAPSIRRPPSARLPLGDRVAAAVLERVLGGLEGGTLAVTLP
                     DGSVRRFGNGPAAEIVLHTPGLLRRLATRPKLALGESYAAGEWSSPDLVRLLELLFAN
                     AEEAAARHPGLRRLADARPRLGGRDGLLAARRNIAYHYDLGNDLFELFLDGTMSYSCA
                     VFAREDEPLVWAQRRKLRRVCERLELGPDDHLLEIGCGWGGLALVAAGEYGARVTGVT
                     ISAAQAELARRRVAEAGLENRVEIVERDFRELDGRYTKIASVEMVEALGEPLLREFFQ
                     VCDRVLEPGG"
     gene            complement(856..1590)
                     /locus_tag="VFO88_05525"
     CDS             complement(856..1590)
                     /locus_tag="VFO88_05525"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HET9545016.1"
                     /translation="MRSALYTGTLVHARHEPKEHVFRYPVAYWLLDLDELDELARLPL
                     VSVNRRGLVSFRDRDHFDGDGTPLKEAVRRFARDPGIERVLCLTQLRVAGYVFNPVSF
                     YWNYGADGALRSMVAELNNTFGERLPQLLPGSALRYEQDKRLHVSPFFGLDQRYRYTF
                     SEPGETLFARIEVEEDGRMPFQAVLAGRRTELSARAVARFLVRYPLMPAQVTAKIHLQ
                     ALRLYLKGVPFRHKPPFVPGKGSVRT"
     gene            complement(1587..2798)
                     /locus_tag="VFO88_05530"
     CDS             complement(1587..2798)
                     /locus_tag="VFO88_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9545017.1"
                     /translation="MQVAVVGGGVAGLGAAWALARRHDVTLFEREPKAGGHVNTVVHD
                     GLALDTGFIVSNERNYPLLGRLFRELDIATQPSEMSFSVTCADCGLEYSGRRPFAQGR
                     NAASPRFLGLLGEIGRWLRTANRSLEEGDWERRSLAEYVDARRYSERFRRHFLVPLSA
                     ALWSAPPGRALELPASYAIRFFANHGMLGLRRFRWQTVAGGSHRYVAAIAARLGEGLR
                     TGVPVRSLRRGFAGVDVRTDDDHIRRFDAVVIAAHADDALALLEDPSREEREILGAFA
                     YSRNTATLHTDSSFLPRASGARASWNYRLGDEGRATVSYWLNRLQRLDAERDYLVTLN
                     EPVPEEHTIETFEYDHPLFTVQTYAAQGRLGELSGRRATEFAGAYHGNGFHEDGLASG
                     LRAAAALGAPW"
     gene            complement(2798..3715)
                     /locus_tag="VFO88_05535"
     CDS             complement(2798..3715)
                     /locus_tag="VFO88_05535"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET9545018.1"
                     /translation="MTEAAIGTVRERTSRLSQLLTLLAVVVPPLGVLSAAGVLWGVAF
                     SPLDLILLLAFYVVCAFGTTIGFHRLFTHRGFETSGPVRAVFAVLGCMTMQGPLTQWV
                     TDHRRHHALSDKPGDPHSPHVGHGDGLWGTVRGFVHAHVGWLFTQKGMERGREYGKDL
                     YEDRLLVWIDRLYIVWVALTLGLPFLLGLWIGGSWERGVQAFVWAGLVRIFLYQHATF
                     AVNSVCHMWGRRHFRSRDEARNNWVIALLVFGEGWHNNHHAFPASARHGLRRFQLDPS
                     WWVIRGLERLGLVWDVKVPSPAQLERRSA"
     gene            complement(3712..4362)
                     /locus_tag="VFO88_05540"
     CDS             complement(3712..4362)
                     /locus_tag="VFO88_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9545019.1"
                     /translation="MNETLLPTGAELRLNNPNGQSAVVCVNGGQSAEVEGTWSATLEW
                     LVGRLAPRFPSLAFGEVRYRVKSWRRLDRCEADARAAIRATGAARTLLVGFSMGGAVA
                     VRAADEPGVEAVVGLAPWLPDRLDLGNLRGKRLAVFHGQLDRWLPGIPGVSPAHSKAG
                     YERALAGGASGRYELLDGALHGVALRAPWGSPVPLPRAGRWADLVADELDRFAEAA"
     gene            4523..>4918
                     /locus_tag="VFO88_05545"
     CDS             4523..>4918
                     /locus_tag="VFO88_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9545020.1"
                     /translation="MSAEPTRTRPRFPSSYGIAADAEGMLDWSWAEERLAASRNYWIV
                     TAREDGSPHAAPVWGLWYDGGVVFGTAPDSRKAVNLRRDRRVCVHLESGDEVVIIEGL
                     AENTHFDEDMADLYAAKYDFRPDTGPGWWR"
BASE COUNT          723 a         1783 c         1809 g          603 t
ORIGIN      
        1 tcccgcccgg ctcgagcacg cggtcgcaga cctggaagaa ctcgcgcagg agaggctcgc
       61 cgagcgcctc gaccatctcg accgaggcga tcttcgtgta cctcccgtcg agctcgcgga
      121 agtcgcgctc gacgatctcc acgcggttct cgagcccggc ctcggccacg cgcctccggg
      181 cgagctcggc ctgcgccgcg gagatcgtca cgccggtgac gcgcgctccg tactctcccg
      241 cggcgacgag cgcgagcccg ccccagccgc agccgatctc gaggaggtgg tcgtccgggc
      301 cgagctcgag gcgctcgcag acgcggcgga gcttgcggcg ctgcgcccag acgagcggct
      361 cgtcctcgcg cgcgaagacc gcgcaggagt acgacatcgt cccgtcgagg aagagctcga
      421 agaggtcgtt cccgaggtcg tagtggtagg cgatgttccg ccgggccgcg aggagcccgt
      481 cgcgcccgcc gagccgcggc cgcgcgtcgg cgaggcgccg cagccccggg tggcgtgccg
      541 ccgcctcctc ggcgttcgca aagagcagct cgagcagccg gacgagatcc ggagagctcc
      601 attcgccggc cgcgtacgac tccccgagcg cgagcttcgg gcgcgtcgcg aggcgccgga
      661 ggagcccggg cgtgtggagg acgatctccg ccgcggggcc gttgccgaac cgccgcaccg
      721 agccgtccgg gagcgtgacg gcgagcgtcc cgccctcgag cccgccgagg acgcgctcga
      781 gcaccgccgc cgcgacgcgg tcgccgagcg gcagccgtgc ggacggcggg cggcggatcg
      841 acggagcggt cgccgtcacg tccgcaccga ccccttcccc gggacgaacg gcggcttgtg
      901 ccggaacggg acgcccttca ggtacagccg caacgcctgg aggtggatct tcgccgtcac
      961 ctgggccggc atgagcggat accgcacgag gaagcgcgcc accgcccgcg ccgagagctc
     1021 cgtacgccgt ccggcgagca cggcctggaa cggcatgcgc ccgtcctcct cgacctcgat
     1081 ccgcgcgaag agcgtctcgc cgggctccga gaacgtgtac cggtaccgct ggtcgaggcc
     1141 gaagaacggc gagacgtgca gccgcttgtc ctgctcgtag cggagcgcgc tcccgggcag
     1201 gagctgcggc agccgctcgc cgaacgtgtt gttgagctcg gcgaccatcg agcggagcgc
     1261 gccgtcggcg ccgtagttcc agtagaagga gaccgggttg aagacgtagc ccgccacgcg
     1321 cagctgcgtg aggcagagga cgcgctcgat ccccgggtcg cgggcgaacc ggcgcacggc
     1381 ctccttgagc ggggttccgt ccccgtcgaa gtggtcgcgg tcgcggaagg agacgaggcc
     1441 ccgccggttg accgagacga gcggaaggcg cgcgagctcg tccagctcgt cgaggtcgag
     1501 gagccagtag gcgaccgggt agcggaagac gtgctccttc ggctcgtgcc gcgcgtggac
     1561 gagcgtaccg gtgtagagcg ccgacctcac cagggagctc ccagcgcggc ggccgcgcgc
     1621 aggcccgagg cgagcccgtc ctcgtggaag ccgtttccgt ggtaggcgcc cgcgaactcg
     1681 gtcgcgcgtc gcccggacag ctcgccgagc cggccctgcg ccgcgtacgt ctggacggtg
     1741 aagagcggat ggtcgtactc gaaggtctcg atcgtgtgct cctccggcac gggctcgttc
     1801 agggtgacga ggtagtcgcg ctccgcatcg aggcgctgga ggcggttgag ccagtacgag
     1861 acggtcgcgc gcccctcgtc gccgagccgg tagttccacg aggcccgcgc gcccgacgcg
     1921 cgtgggagga acgacgagtc agtgtgcagc gtcgcggtgt tccgcgagta cgcgaacgcg
     1981 ccgaggatct cccgctcctc gcggctcggg tcctcgagaa gggcgagcgc gtcgtcggcg
     2041 tgggcggcga tcacgacggc gtcgaagcgg cggatgtggt cgtcgtccgt tcgcacgtcg
     2101 actcccgcga agccccggcg gaggctgcgc accggcaccc cggtgcgcag cccctcgccg
     2161 agccgcgccg cgatcgcggc gacatagcgg tggctgccgc cggccaccgt ctgccagcga
     2221 aagcgacgca ggcctagcat cccgtggttc gcgaagaagc ggatcgcgta cgaggccggc
     2281 agctcgagcg cgcggcccgg cggcgccgac cagagcgcgg ccgagagcgg cacgaggaag
     2341 tggcgccgga accgctcgga gtaccgccgc gcgtcgacgt actccgcgag cgaccggcgc
     2401 tcccagtcgc cctcctcgag cgagcggttg gccgtccgga gccagcggcc gatctccccg
     2461 agcagcccga ggaagcgggg gctcgctgcg ttccgcccct gcgcgaacgg ccgccggccc
     2521 gagtactcga gcccgcagtc cgcgcacgtg accgagaagg acatctccga cggctgcgtc
     2581 gcgatgtcga gctcgcggaa gagccgcccg agcagcgggt agttgcgctc gttcgagacg
     2641 atgaaccccg tgtcgagcgc gagcccgtcg tggacgaccg tgttcacgtg cccgcccgcc
     2701 ttcggctccc gctcgaagag cgtcacgtcg tgccggcgcg cgagcgccca cgccgctccg
     2761 agcccggcca cgccgccgcc gacgaccgcg acctgcatca ggccgaccgc cgctcgagct
     2821 gcgccgggct cggcaccttc acgtcccaga cgagcccgag cctctcgagc ccgcggatca
     2881 cccaccagga cgggtcgagc tggaaccgcc gcagcccgtg ccgcgcggac gcggggaagg
     2941 cgtggtggtt gttgtgccag ccctccccga agacgagcag cgcgatcacc cagttgttgc
     3001 gcgcctcgtc ccgcgaccgg aagtgccgcc gcccccacat gtggcagacg gagttcacgg
     3061 cgaacgtcgc gtgctggtag aggaagatcc gcacgagccc ggcccagacg aacgcctgca
     3121 cgccgcgctc ccagctcccg ccgatccaga gcccgagcag gaacggaagg ccgagcgtga
     3181 gcgcgaccca gacgatgtag agccggtcga tccagaccag caggcggtcc tcgtagagat
     3241 ccttgccgta ctcgcggccg cgctccatcc ccttctgcgt gaagagccag ccgacgtgcg
     3301 cgtggacgaa cccgcgcacc gttccccaga ggccgtcccc gtggccgacg tgcggcgagt
     3361 gcgggtcgcc cggcttgtcg gagagcgcgt ggtggcgccg atggtccgtc acccactgcg
     3421 tgagcgggcc ctgcatcgtc atgcacccga gcaccgcgaa gaccgcgcgc accggtccgg
     3481 acgtctcgaa cccgcggtgc gtgaagagcc ggtggaagcc gatcgtcgtc ccgaacgcgc
     3541 agacgacgta gaacgcgagg agcaggatca ggtccagcgg ggagaaggcg acgccccaga
     3601 ggacgccggc cgccgagagg acgccgagcg gcgggacgac gacggcgagc agcgtcagga
     3661 gctggctgag ccgcgaggtg cgttcgcgga ccgttccgat tgccgcctcg gtcatgcggc
     3721 ctcggcgaag cggtcgagct cgtccgcgac gaggtcggcc cagcgtccgg cgcgggggag
     3781 agggacgggc gagccccagg gggcgcggag cgcgacgccg tgcagcgcgc cgtcgagcag
     3841 ctcgtagcgg ccgctcgcgc cgccggccag cgcccgctcg taccccgcct tcgagtgggc
     3901 ggggctgaca ccggggatgc ccggcagcca acggtcgagc tgcccgtgaa agacggccag
     3961 gcgcttgccg cggaggttcc cgaggtcgag gcggtcgggc agccagggag ccagaccgac
     4021 gacggcctcg acgccgggct cgtcggccgc ccgcaccgcg accgcgccgc ccatcgagaa
     4081 gccgacgagc agcgtccgcg cggcgccggt cgcccggatc gcggcgcgag cgtccgcctc
     4141 gcagcggtcg aggcgccgcc acgacttcac ccggtagcgc acctcaccga aggcaagcga
     4201 cggaaagcgc ggcgcgagcc ggccgacgag ccattcgagc gtggcgctcc acgtgccctc
     4261 gacctcggcg ctctgcccgc cgttcacaca gacgacggcg ctctggccgt tcgggttgtt
     4321 gagccgcagc tcggcgccgg tggggaggag cgtttcattc acagtttgtc catgatatgt
     4381 acaacccttg tccgcttgcg tcggtcctta cacctccttt gatgtctcgc ccgagcgcgg
     4441 ttgaatcccc gcggtccgcc tgcgcaccgc cgcgcgccgg tgaggcgccc tcgacggcag
     4501 gcggcgcgat tatgctcgcc gcatgtccgc cgagcccaca cgcacccgcc cgcgcttccc
     4561 gtcgagctac gggatcgcgg ccgacgcgga agggatgctc gactggtcgt gggccgagga
     4621 gcgcctcgcg gcgtcgcgaa actactggat cgtcacggcg cgcgaggacg ggagccccca
     4681 cgctgcgccg gtctgggggc tctggtacga cggcggggtc gtgttcggga cggcgcccga
     4741 ctcgcgcaag gcggtgaacc tgcgccgcga caggcgcgtc tgcgtccacc tcgagagcgg
     4801 ggacgaggtc gtgatcatcg agggcctcgc cgagaacacc cacttcgacg aggacatggc
     4861 cgacctctac gcggcgaagt acgacttccg gccggacacc gggcccgggt ggtggcgc
//
LOCUS       DASTFC010000183         5133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1058571,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000183 DASTFC010000000
VERSION     DASTFC010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058571"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFO88_05550"
     CDS             complement(<1..546)
                     /locus_tag="VFO88_05550"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9545021.1"
                     /translation="MADRVTLVVPEGAAGERLDRFLAGVEAVGSRSAAERLLAAGTVT
                     VAGAPRPKSHRLEGGEEVAFDAPEAVASSLEPDPGVPLRIAYEDEQLLVVDKPAGIVV
                     HPSAGHAGGTLVHGLLAHAIAGGEEAERPGIVHRLDRDTSGLLVVARSDAVHRALQRS
                     LRAREITREYLALVRGRPRSRR"
     gene            complement(539..1063)
                     /gene="lspA"
                     /locus_tag="VFO88_05555"
     CDS             complement(539..1063)
                     /gene="lspA"
                     /locus_tag="VFO88_05555"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9545022.1"
                     /translation="MPISVAERSLSAGPYEWIALVSIALAALVADQVTKVIVVRSLEL
                     GESVTVLGPLTINHVRNSGIAFGLFSSWAGTVTILTAAAVLWMVFYFARAGARHPILP
                     IALGLLIGGSAGNLVDRIRLGHVTDFLDLTYWPAFNLADTFIVTGVGILMVALVAAER
                     KPRVHGPRPSRGGG"
     gene            complement(1120..1482)
                     /locus_tag="VFO88_05560"
     CDS             complement(1120..1482)
                     /locus_tag="VFO88_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET9545023.1"
                     /translation="MAGVDTDRFRTLLLEEREKVERALGNLHDENPGSIEDEEEETFD
                     NHLGDAATITFDRELDSTLEENSGRMLAAIDEALKRLEEGTFGTCARCGQPIAEARLE
                     AMPYATKCIDCKRLEERG"
     gene            complement(1526..1999)
                     /locus_tag="VFO88_05565"
     CDS             complement(1526..1999)
                     /locus_tag="VFO88_05565"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET9545024.1"
                     /translation="MTLTPVEIRHVRLPRRVLGYARPAVDDLLEDVVRSFEAAWRDRA
                     DLRDEVEKLEGEVARYKEVDVLLRNSLVSAEKAADELRAHAGKEADVILEEARIRARE
                     ITAEAEAERERIRGEVRRLRALEADARAGYRAFLLTALDRLEGDTEERIRPDQAA"
     gene            complement(2010..2312)
                     /locus_tag="VFO88_05570"
     CDS             complement(2010..2312)
                     /locus_tag="VFO88_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET9545025.1"
                     /translation="MVLADTITTAQNFVQTLFYVYLALVLAYIITSWIPLPYSVWLNR
                     IQRFLYDVVDPYLRLFRRFLPALRLGGAGLDLSPIVAIIVLYVLNTVIQEGLELLR"
     gene            complement(2480..3034)
                     /gene="sepF"
                     /locus_tag="VFO88_05575"
     CDS             complement(2480..3034)
                     /gene="sepF"
                     /locus_tag="VFO88_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET9545026.1"
                     /translation="MGVGDVWNRALVYFGIVEEEQYWDDEAFDAEEELDRGYRERERP
                     NVRRLHAGRRRGAEFEDWSDPDDVQPTAVLRQGGGSSRRAPLEAVAAASVRVHLVVPR
                     NFDDAKPVADKFKDAVPVILNLQGTDPDLSKRLIDFSSGLTYALNGGMQRIADKVFLL
                     TPRNVEVSAEERARLAERGFFNQS"
     gene            complement(3048..3659)
                     /locus_tag="VFO88_05580"
     CDS             complement(3048..3659)
                     /locus_tag="VFO88_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9545027.1"
                     /translation="MPLSPELVRENLARIRDEIGPGPTIVAATKYVAVEDMPALAEAG
                     VEVVGENRLQDLAAKHALYGDAFRWHFIGHLQSRKARDVSALCELLHSLDSLKAARRV
                     EIPALVEVNLSGEETKGGVPPEELGAFLAGVRELGVDVRGLMTMPPLAADPEESRPYF
                     RRLRELAGEHGLAELSMGTSQDYRVAAEEGATLVRIGSILYGR"
     gene            complement(3643..4356)
                     /locus_tag="VFO88_05585"
     CDS             complement(3643..4356)
                     /locus_tag="VFO88_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET9545028.1"
                     /translation="MLWQGAPAGVRAAFTTRLGGVSEGPFASLNLGLLTDDEPERVVE
                     NRRRACEAAGVDAESATMALQVHGARVTEARPTGVVTPGTDYEPCDGLWSDRPGQGML
                     LVTADCLPVALARPDGAAPLAVLHVGWRGLLAGIAEEGARVVGAGAAAAIGPGIGPCC
                     YEVGEEVAAPYRARFGARVVRDGRLDLPLATELALREAGVASVERVGGCTACDPARFF
                     SHRRDRGRTGRQGIVAALA"
     gene            complement(4362..4985)
                     /locus_tag="VFO88_05590"
     CDS             complement(4362..4985)
                     /locus_tag="VFO88_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9545029.1"
                     /translation="MSRLELFLWLVPLLVTSMVLHELAHAVVATRLGDPTPREHGRLT
                     LNPVSHLDPLGTAMFGITYWLSSFMFGWAKPVLVQPGYFRRPKEGMALVAVAGPLTNF
                     AIALVVVAVLIHVELSDRAFEVLALLYQVNVILGVFNLLPVPPLDGSRIVAVAMSDAT
                     AARWRGLDQYGMFAIFGLIFVFRDAFFELIGGAYEEITSLMVTAVGG"
BASE COUNT          737 a         1892 c         1830 g          674 t
ORIGIN      
        1 ccgtcgcgag cgcggccgcc cgcgaacgag cgcgaggtac tcacgcgtga tctcgcgggc
       61 ccgcagcgag cgctggagcg cccggtgcac ggcgtccgag cgggcgacga cgagcagccc
      121 cgacgtgtcc cggtcgaggc ggtggacgat cccgggccgc tcggcctcct ccccgcctgc
      181 gatcgcgtgt gcgaggagcc cgtggacgag cgtgccgccg gcgtgtccgg ccgagggatg
      241 gacgacgatg ccggccggct tgtcgaccac gagcagttgt tcgtcctcgt aggcgatccg
      301 gagcgggacg ccggggtccg gctcgagcga ggacgcgacg gcctcgggcg cgtcgaacgc
      361 cacctcctcc ccgccctcga gccggtggct cttcggccga ggcgcgcccg cgaccgtcac
      421 cgtcccggcc gcgagcaacc gttccgccgc cgagcgcgag ccgaccgcct cgactcccgc
      481 gagaaaccgg tcgagccgct cgccggccgc cccctcgggg acgacgagcg tgacccggtc
      541 agccaccgcc gcgcgacggc cgcgggccgt gcacgcgcgg cttccgctcg gcggcgacga
      601 gcgcgaccat gaggatcccc accccggtca cgatgaaggt gtccgcgagg ttgaacgccg
      661 gccagtacgt caggtcgagg aagtccgtca cgtgcccgag ccggatgcgg tcgacgaggt
      721 tccccgcgct tcctccgatc agcaggccga gcgcgatcgg aaggatcggg tgccgcgcgc
      781 ctgcgcgcgc gaagtagaag accatccaga gcacggccgc ggcggtcagg atcgtcacgg
      841 tgccggccca gctcgagaag agcccgaacg cgatgccgga gttacggacg tggttgatcg
      901 tgagcgggcc caggacggtc accgactcgc cgagctcgag gctgcgcacg acgatcacct
      961 tcgtcacctg gtcggcgacg agcgccgcga gggcgatcga gacgagcgcg atccactcgt
     1021 acggcccggc ggagagcgag cgctccgcca ccgagatggg cacgcgcccg tcggtcgcgg
     1081 accccacccg cacgtcgagc gcgcgggaag gctcggtcgt cagccccgct cttcgagccg
     1141 tttgcagtcg atgcatttcg tcgcgtacgg catcgcctcc aggcgtgcct ccgcgatcgg
     1201 ctggccgcag cgggcgcacg tgccgaacgt cccctcctcg agtcgcttga gcgcttcgtc
     1261 gatcgccgcg agcatccgcc ccgagttctc ctcgagggtc gagtccagct cgcggtcgaa
     1321 cgtgatcgtg gccgcgtcgc cgaggtggtt gtcgaacgtc tcctcctcct cgtcctcgat
     1381 cgaccccggg ttctcgtcgt gaaggttccc gagcgcgcgc tcgaccttct cccgctcttc
     1441 gagcaggagc gtacgaaaac gatcggtgtc gaccccggcc acgggctgag tatatcgccg
     1501 ggggtggccg acccccgggt gcccgtcagg cggcctggtc gggccgaatg cgctcctcgg
     1561 tgtccccctc gagccggtcg agcgcggtca ggagaaacgc ccgatagccg gcccgggcgt
     1621 cggcctcgag cgcgcgcagg cggcgcacct ctccccggat ccgctcgcgc tcggcctccg
     1681 cctccgcggt gatctcgcgg gcgcggatcc gggcctcctc gaggatcacg tcggcttcct
     1741 tcccggcgtg cgcgcggagc tcgtcggccg ccttctcggc cgagacgagg ctgttccgca
     1801 ggaggacgtc gacctccttg tagcgggcga cctcgccctc gagcttctcg acctcgtcgc
     1861 ggaggtcggc gcggtcgcgc cacgcggcct cgaaggaccg gacgacgtcc tcgagcaggt
     1921 cgtcgacggc cggccgcgcg tacccgagca cgcgacgcgg caggcggacg tggcgaatct
     1981 cgacgggtgt cagcgtcaca cgtcgaggtt cagcgcagga gctccaatcc ctcctggatg
     2041 acggtgttca ggacgtagag cacgatgatc gcgacgatcg gcgagaggtc gagccccgcg
     2101 ccgccgagcc ggagcgccgg gaggaagcgg cggaagaggc gcaggtacgg gtcgacgacg
     2161 tcgtagagga agcgctggat ccggttcagc cagaccgagt acgggagcgg gatccaggac
     2221 gtgatgatgt acgcgagcac gagcgcgagg tagacgtaga acagcgtctg gacgaagttc
     2281 tgcgccgtcg tgatcgtgtc ggcgaggacc atgggcgcac cctacttcgc ctcgaggtcg
     2341 gcgggcaacg cggctgccgg cgaagacgtc tgccgccgcg cccgacatgg cgggcgcggc
     2401 tcaccgacgt agaggattgg gcggtttccc cgccggagat cgtcatgccc cgccgcaggc
     2461 ggggcggatc tctcggcggc tacgactggt tgaagaagcc gcgctccgcg aggcgggcgc
     2521 gctcctccgc ggacacctcg acgttcctgg gcgtcaggag gaagaccttg tccgcgatcc
     2581 gctgcatccc cccgttcagc gcgtacgtga gcccgctcga gaagtcgatg agccgcttcg
     2641 agagatccgg gtcggtgccc tggaggttca ggatgaccgg cacggcgtcc ttgaacttgt
     2701 cggcgaccgg cttcgcgtcg tcgaagttcc gcggcactac gaggtggacg cgcaccgagg
     2761 ccgcggcgac cgcctcgagg ggcgcgcggc gggacgaccc tccgccctgc cggaggacgg
     2821 cggtcggctg gacgtcgtcc gggtcgctcc agtcctcgaa ctcggcgccg cgccggcgtc
     2881 ctgcgtggag ccgccgcacg ttcggccgct cgcgctcgcg gtagccgcgg tcgagctcct
     2941 cctccgcatc gaaggcctcg tcgtcccagt actgctcctc ctcgacgatg ccgaagtaga
     3001 cgagcgcgcg gttccagacg tcgccgacac ccatgcggcg agaaagctta ccggccgtac
     3061 aggatcgaac cgatgcgcac gagcgtcgcc ccctcctcgg ccgccacgcg gtagtcctgc
     3121 gacgtcccca tcgagagctc cgcgaggccg tgctcgcccg ccagctcgcg gagtcggcgg
     3181 aagtacgggc gcgactcctc ggggtcggcg gcgagcggcg gcatcgtcat gaggccgcgg
     3241 acgtccacgc cgagctcgcg gacgccggcg aggaacgcgc cgagctcctc cggcgggacg
     3301 ccgcccttcg tctcctcgcc cgagaggttc acctcgacga gcgcggggat ctcgacgcgc
     3361 cgcgcggcct tgagcgagtc gagcgagtgg agcagctcgc agagcgccga cacgtcccgc
     3421 gccttccgcg actggagatg cccgatgaag tgccagcgaa aggcgtcccc gtagagcgcg
     3481 tgcttcgcgg cgagatcctg gagccggttc tcgcccacca cctccacccc ggcctcggcg
     3541 agggcgggca tgtcttcgac ggccacgtac ttcgtcgcgg cgacgatcgt cgggcccggg
     3601 ccgatctcgt cgcggatccg ggcgaggttc tcgcgcacga gctcaggcga gagcggcaac
     3661 gatcccctgc cttccggtcc ggccgcggtc gcggcgatgt gagaagaacc gcgccgggtc
     3721 acacgccgtg cagccgccga cgcgctcgac ggaggcgacg cctgcctccc gcagcgcgag
     3781 ctcggtcgcg agcgggaggt cgagccgccc gtcgcgcacg acccgcgcgc cgaaccgcgc
     3841 ccggtacggc gcggcgacct cctcccccac ctcgtagcag cacggcccga tccccggccc
     3901 gatcgccgcg gccgcgccgg cgccgaccac acgcgccccc tcctcggcga ttcccgcgag
     3961 caggccgcgc cagccgacgt ggaggacggc gagcggcgcc gcgccgtccg gccgcgcgag
     4021 cgcgacgggc aggcagtccg ccgtgacgag cagcatcccc tgcccgggcc ggtcgctcca
     4081 caggccgtcg cacggctcgt agtccgtccc gggcgtcacg acgccggtcg gccgcgcctc
     4141 cgtgacgcgc gcgccgtgca cctggagggc catcgtcgcg ctctccgcgt cgacccccgc
     4201 ggcctcgcac gcgcgccggc ggttctcgac cacgcgctcc ggctcgtcgt ccgtgaggag
     4261 gccgaggttg agcgacgcga acgggccctc gctcacgccg ccgagccgcg tcgtgaacgc
     4321 cgcacgcacg ccggccggcg ctccctgcca cagcatcgcg ctcacccgcc gaccgccgtg
     4381 accatgaggc tcgtgatctc ctcgtaggcg cctccgatca gctcgaagaa cgcgtcgcgg
     4441 aagacgaaga tgagcccgaa gatcgcgaac atcccgtact ggtcgagccc ccgccatcgc
     4501 gcggcggtcg cgtccgacat cgcgacggcg acgatccggg atccgtccag cgggggcacc
     4561 gggaggagat tgaacacacc gaggatcacg ttcacctggt agaggagcgc aagcacctcg
     4621 aaggcgcggt ccgagagctc cacgtgaatc aggacggcga cgacgacgag cgcgatcgcg
     4681 aagttcgtga gcggccctgc gaccgcgacg agcgccatcc cctccttcgg tcggcggaag
     4741 taccccggct gcacgagcac cggcttcgcc cagccgaaca tgaagctcga gagccagtac
     4801 gtgatcccga acatcgccgt gccgagcggg tcgaggtgcg agaccgggtt gagcgtgagg
     4861 cgcccgtgct cccgtggggt cgggtcgccc aaccgcgtcg cgacgacggc gtgggcgagc
     4921 tcgtgcagca ccatgctcgt cacgagcagc gggacgagcc agaggaaaag ctcgagccgg
     4981 ctcacgcggt ttcgaggagc tcccgggccg cctcgagctc ggccgagcgg ccgtccagct
     5041 ccccgttcag cttgcgccgg agcagctcgt cgaggatctc cccgacgcgc ggcgactcgg
     5101 cgacgccgag cccggccagg tcgccgccgg tga
//
LOCUS       DASTFC010000184         4699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1338501,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000184 DASTFC010000000
VERSION     DASTFC010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338501"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFO88_05595"
     CDS             complement(<1..357)
                     /locus_tag="VFO88_05595"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET9545030.1"
                     /translation="MPELAVSSAYSPTGDQPEAIRDLAQSVEAGNRFQTLLGATGTGK
                     TATMAWTIEKLRRPTLVIAHNKTLAAQLCNEFREFLPHNAVEYFVSYYDYYQPEAYVP
                     QADLYIEKDASVNDDID"
     gene            834..2264
                     /locus_tag="VFO88_05600"
     CDS             834..2264
                     /locus_tag="VFO88_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9545031.1"
                     /translation="MRTRLLTGLAALASVAAAALPASPAAAAPERPNIVLVLTDDQRW
                     DTLDAMPTVMRELVGRGVTFENAFAVNPTCCPSRATILTGDHSHSTGVWANGGPHGGS
                     DWFDHASTIAPLLQEQGYRTAYLGKYLNGYGGWAPPFLDRWFRPPGWDTWLGNGGGYV
                     GYPVQVAGLMTRHEGDERYLTDVLASAAVSFLQEDETTPFFLVVAPYAPHHPAVPAER
                     HAGAFAAEPPFRPPAYDETDVSDKPRWVRQQPRLSEARAERLDALRRRQLETLLAVDE
                     GVARILGALRASNRLESTLVVFAADNGLHWGEHRLMNRKSTPYEESIRIPLVVRWDAL
                     VDRPRREARIVGNLDLAPTFADAAGAATDGMDGASLLPLLARGPGDAPSWRSGILLES
                     MPGEGEASAEIPAYCAVRDARWKYVVYATRDEELYDLAADPDELENLAFLPEHRARRA
                     ALRAELLRLCRPPPPGLDLEWLPSDG"
     gene            2282..4087
                     /locus_tag="VFO88_05605"
     CDS             2282..4087
                     /locus_tag="VFO88_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9545032.1"
                     /translation="MSRRPRTGLLPLVCVLAAAVALAAAPPAGAQAAKPDIVLVLTDD
                     QRWDSLDAMPTVTSELVEKGVTFTNAFVSNPLCCPSRASILTGLHSHSTLVYTNAKPY
                     GGYSWFDDSSTVATWLRRAGYRTGYVGKYLNGYTVPDVPRGWDRWVGYWNGFYGYTVS
                     VDGVYRSYGFAPDDYSTDVFAGEAVDFVEQAGDGPLFLVYAPYAPHTPSTPAPRHLSA
                     FPGWTPPRAPSYDEANVRDKPRWVRRTPRITPESGAALDELALRMRRSLLAVDDGIGA
                     ILDALDGAGRLENTLLVFTSDNGLMWGEHRQLGMKVAPYEESIRVPFVVRWDALGLGA
                     REEPRFASNLDLAPTFAAVAGARRPAVEGRSLVPILGGDPARWRSQVLLEHLRGGTNR
                     ATGVPTYCGVRGGRWKYAVYETKEEELFDLAADPHELRSVHRAAAHRARLLQLRRDVR
                     RLCDPAPPGFSLTWLCTHETTAGRRTATGTRRADTLCGRARADRLFGRGGNDVLKGRA
                     GNDALAGGGGNDRLEGGAGRDRLTGGPGRDTLAGGPGRDTISGGGGRDLVRARDGARD
                     RIACGPGRDVVYADTSDVVAADCEDVRRPRPPRGL"
     gene            complement(4084..4638)
                     /locus_tag="VFO88_05610"
     CDS             complement(4084..4638)
                     /locus_tag="VFO88_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9545033.1"
                     /translation="MRYAVATLLAVAGLAAAAVALERSQPAWYAKLRYPLSYEHVIVG
                     HAENYELDAALIAAVIYRESKFDPDAESSSGAIGLMQLLPSTAEGIALNTGGSRFVVR
                     DLYDPEINVRYGSFYLRRLLRKYDDTRLALAAYNAGQANVDEWLQADEGIAFPETREY
                     VDDVLALREVYARAYDEELELDEG"
BASE COUNT          599 a         1821 c         1690 g          589 t
ORIGIN      
        1 gtcgatgtcg tcgttcacgg acgcgtcctt ctcgatgtag aggtcggcct gcgggacgta
       61 cgcctccggc tggtagtagt cgtagtacga gacgaagtac tcgaccgcgt tgtgcgggag
      121 aaactcgcgg aactcgttgc agagctgcgc ggcgagcgtc ttgttgtgcg cgatgacgag
      181 ggtcgggcgg cggagcttct cgatcgtcca cgccatcgtg gccgtcttgc ccgtaccggt
      241 cgcgccgagg agcgtctgga agcggttccc cgcctcgacc gactgggcga ggtcgcggat
      301 cgcctccggc tgatcgccgg tgggcgagta ggcgctggag acggcgagct ctggcatcgc
      361 gccatcgtag cccccggccg cggcgccgcg aaccgcccgg cccgcggcgc gtccccgggg
      421 gccacgaccc gtgcctggca ccggtcgtgg ccccgccgga cgcgtccggg aacggcacac
      481 cgtcccggcc ggacgccggc cggagcggcc tcgtccgctt acccgcaagg ccgcatccgc
      541 gctggccggc cacgtccccg gcggccccgg ccggtgcctg gcaccggaca cggcccggcc
      601 ggacgtgccc gggaacggca catcgtcccg gccggacggc ggccggagcg gcctcgtccg
      661 cgtacccgca aggccgcatc cgcgctggcc ggccacgccc ccggcggcca cgaccggtgc
      721 ctggcaccgg acacggcccg gccggacgcg cccgggaacg gcgccccgcc ccgagcggac
      781 gcgggcggcg cggccggtcg ggggcgagac tagactcgaa ggccggttcc gccgtgcgga
      841 cacgccttct caccggcctc gccgcgctcg cgtccgtcgc ggccgcggcg ctcccggcgt
      901 cccccgccgc cgcggcgccc gagcggccga acatcgtcct cgtgctgacc gacgaccagc
      961 gctgggacac gctcgacgcg atgccgaccg tcatgcgcga gctcgtcggc cgcggcgtca
     1021 cgttcgagaa cgcgttcgcc gtcaacccga cctgctgccc cagccgggcg acgatcctca
     1081 ccggcgacca ctcgcactcg accggcgttt gggcgaacgg cggcccgcac ggaggctccg
     1141 actggttcga ccacgcgtcg acgatcgcgc cgctcctgca ggagcagggc taccggacgg
     1201 cctacctggg caagtacctg aacggctacg gcgggtgggc gccgcccttc ctcgaccgct
     1261 ggttccggcc gcccggctgg gacacgtggc tcggcaacgg cggcggctac gtcggctacc
     1321 cggtccaggt cgccggcctc atgacccgcc acgagggcga cgagcgctac ctcacggacg
     1381 tcctcgcgag cgcggccgtc tcgttcctcc aggaggacga gacgacgccg ttcttcctcg
     1441 tcgtcgcgcc gtacgcgccc caccatcccg ccgtcccggc ggagcggcac gcgggcgcct
     1501 tcgccgccga gccgccgttc cgcccgcccg cgtacgacga gaccgacgtg tccgacaagc
     1561 cccgctgggt gcgccagcag ccgcggctct ccgaggcgcg ggccgagcgg ctcgacgcgc
     1621 tccgccgccg ccagctcgag acgctgctcg ccgtcgacga gggcgtcgcg cggatcctcg
     1681 gcgccctgcg cgcgagcaac cggctcgaga gcacgctcgt cgtgttcgcg gccgacaacg
     1741 ggctccactg gggcgagcac cgcctgatga accggaagag cacgccgtac gaggagagca
     1801 tccggatccc gctcgtcgtc cgctgggacg cgctcgtcga ccggccgcgc cgcgaggccc
     1861 gtatcgtcgg caacctcgac ctcgcgccga cgttcgccga cgcggccggc gcggccacgg
     1921 acggaatgga cggcgcgagc ctcctccccc tgctcgcgcg aggccccggc gacgccccga
     1981 gctggcgcag cgggatcctc ctcgagtcca tgcccggcga gggcgaggcg tcggccgaga
     2041 tccccgcgta ctgcgccgtc cgcgacgcgc gctggaagta cgtcgtctac gccacgcgcg
     2101 acgaggagct ctacgacctc gccgccgacc ccgacgagct cgagaacctc gccttcctcc
     2161 cggagcaccg cgcgcgccgc gccgcgctcc gcgccgagct cctccgcctg tgccgcccgc
     2221 cgccgcccgg gctcgacctg gagtggctcc caagcgacgg ttagactcgc gcgcaggcca
     2281 gatgtcgcgt cgcccacgca ccggcctgct tccgctcgtc tgcgtcctcg cagcggcggt
     2341 cgcgcttgcc gcagcgcccc cggcgggcgc ccaggcggcg aagccggaca tcgtcctcgt
     2401 gctcacggac gaccagcgct gggactcgct cgacgcgatg ccgacggtga cgagcgagct
     2461 cgtggagaag ggcgtcacgt tcacgaacgc gttcgtgtcg aacccgctct gctgcccgag
     2521 ccgcgcgagc atcctgaccg ggctccactc gcactcgacc ctcgtctaca cgaacgcgaa
     2581 gccgtacggc gggtactcct ggttcgacga ctcgtcgacg gtggccacct ggctccggcg
     2641 cgcgggctac cggacgggct acgtcgggaa gtacctgaac ggctacaccg tccccgacgt
     2701 gccgcgcgga tgggaccgct gggtcggcta ctggaacggg ttctacggct acacggtctc
     2761 ggtcgacggc gtctaccggt cctacggctt cgcgccggac gactactcga cggacgtctt
     2821 cgccggcgag gcggtcgact tcgtcgagca ggcgggcgac gggccgctct tcctcgtcta
     2881 cgcgccgtac gcgccccaca cgccctcgac gccggccccg cgccacctct cggcgttccc
     2941 gggctggacg ccgccccgcg cgccgtccta cgacgaggcg aacgtccgcg acaagccccg
     3001 ctgggtgcgc cgcacgcccc ggatcacgcc ggagagcggc gccgcgctcg acgagctcgc
     3061 gctcaggatg cgccgaagcc tcctcgccgt cgacgacggg atcggggcga tcctcgacgc
     3121 gctcgacggg gcgggacggc tcgagaacac gctcctcgtg ttcacgtccg acaacgggct
     3181 catgtggggc gagcaccgcc agctggggat gaaggtcgct ccgtacgagg agagcatccg
     3241 cgtcccgttc gtcgtccgct gggacgcgct cgggctgggc gcgcgcgagg agccgcgctt
     3301 cgcctcgaac ctcgatctcg cgccgacgtt cgcggccgtc gcgggcgcgc gccggccggc
     3361 ggtcgagggg cggagcctcg tcccgatcct cggcggcgac ccggcgcggt ggcggagcca
     3421 ggttctgctc gagcacctgc gcggcggcac gaaccgcgcg accggcgtgc cgacgtactg
     3481 cggcgtgcgc ggaggccggt ggaagtacgc cgtctacgag acgaaggagg aggagctctt
     3541 cgacctcgcc gccgacccgc acgagctgcg cagcgtccac cgcgcggccg cgcaccgggc
     3601 gcggctcctc cagctccgcc gcgacgtgcg gcgcctctgc gacccggcac cgcccgggtt
     3661 ctcgctcacg tggctctgca cgcacgagac gacggccggg cgccgcaccg cgacgggcac
     3721 gcgccgcgcc gacaccctct gcggccgcgc gagggccgac cggctcttcg ggcgcggcgg
     3781 gaacgacgtc ctcaagggac gcgcggggaa cgacgcgctc gcgggcggcg gcgggaacga
     3841 ccggctcgag ggcggcgccg ggcgcgaccg gctcaccgga gggccgggga gggacacgct
     3901 cgccggcggg cccgggcggg acacgatctc gggcggcggc gggcgcgacc tcgtccgggc
     3961 ccgcgacggc gcccgcgacc ggatcgcgtg cgggcccggg cgcgacgtcg tctacgcgga
     4021 cacgagcgac gtcgtcgccg ccgactgcga ggacgtccgc cgcccccgcc cgccgcgcgg
     4081 cctctagccc tcgtccagct cgagctcctc gtcgtacgcg cgtgcataga cctcgcggag
     4141 cgcgagcacg tcgtccacgt actcgcgcgt ctcggggaac gcgatcccct cgtcggcctg
     4201 gagccactcg tcgacgttcg cctggccggc gttgtaggcg gcgagcgcga gccgcgtgtc
     4261 gtcgtacttc cgcagcagcc gccggaggta gaacgagccg tagcggacgt tgatctccgg
     4321 gtcgtagagg tcgcgcacga cgaaccggct cccgcccgtg ttcagcgcga tcccctcggc
     4381 cgtggacggg aggagctgca tgagcccgat cgcgcccgag ctcgactccg cgtccgggtc
     4441 gaacttgctc tcgcggtaga tcacggccgc gatgagcgcc gcgtcgagct cgtagttctc
     4501 ggcgtgcccg acgatcacgt gctcgtacga gagcgggtag cggagcttcg cgtaccaggc
     4561 cggctgcgag cgctcgagcg cgaccgccgc ggccgcgagc ccggcgaccg cgaggagggt
     4621 tgcgacggcg tatctcacga acgggtcagg gtgcccatca cgtcggacac gaagcggtcg
     4681 agctcttcga gcgacccct
//
LOCUS       DASTFC010000185         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1009108,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000185 DASTFC010000000
VERSION     DASTFC010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009108"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2820)
                     /locus_tag="VFO88_05615"
     CDS             complement(<1..2820)
                     /locus_tag="VFO88_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET9545034.1"
                     /translation="MSTTERTRRRGADEPLPAALDRRFEAIVFDWDGTAVPDRAADAA
                     DLRALVEELCERGLDLVVITGTHVGNVDGQLGARPAGPGRLYLCVNRGSEVFVAGEDG
                     VELLDRREATPEEEAALDAAAAATVEELGRRGVAAAIVSKRLNRRKIDLIPEPEWADP
                     PKARIAELLKAVQERLGAAGITLGDAVEIATTIARSAGIQDPRVTSDAKHVEVGLTDK
                     ADAARWAVAELARRGVGPGLTLVAGDEFGPLGGLPGSDSLLLVPEAAAATALTVGAEP
                     SGAPADVLALGGGPEVFLRILADQLERRRRGDVPELDGGSEWTLAVEGLDPALERVHE
                     SLLTLADGRLGTRGAPLFDDPSVERAVLLSGVYTGDGSRTELARAPDWSRLLPAPGPP
                     APSLTRRLDLATGLLREEGPGASLRFSSLARPGTVALRAEADGREVVPPAGDDVRGAV
                     SVAVRERQAGAIVERIAAYDLDEEAARTAVGSAEAAGFERLLREQRHAWASRWAGADV
                     VLEGDPALQKAIRFALFHLMASVGDEGEAAVGARGLTGPAYSGHVFWDSDVFVLPFLA
                     ATHPPAARAMLEYRVRRLPAAREAACALGRAGARFAWESAADGRDVTPPSVRLATGEL
                     VRIRTGELEEHIVGDVAWAVACYLDWTGDEEFASGPGRELLVETARYWASRVRRGADG
                     RAHIYGVIGPDEYHEPVDDNAFTNVLARWNLRRAAALPGPDAGERATWLEVADALVDG
                     YDRASGIYEQFAGFSDLEPLVIAEVAPKRPIAADLLLGAERTAGAQVLKQADVLMLHH
                     LLPEEVPAGSLVPNLDFYEPRTAHGSSLSPAIHASLLARAGRYRPALDALRIAARLDL
                     DDLTGSTAGGLHLATMGGLWQALVHGFAGVRPRGGRLHVDPRLPREWNALELSLRFRG
                     RPLRLRVDRRGVTASPDGWVVEERPD"
     gene            complement(2817..>3375)
                     /locus_tag="VFO88_05620"
     CDS             complement(2817..>3375)
                     /locus_tag="VFO88_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9545035.1"
                     /translation="DGSEGSPNMFGWGSTPDIDAAVAWLDERPDVDDGRIGGIGFSVG
                     GEMMLEAAAGDDGLRAVVSAGAGVRSIREARLYGPRGWLAQPQQLTQTAALAVLSGTA
                     PPPALDDAIARIAPRAVFLVYAAEGQGGEDLTPEFFEAAGEPKEVWRVETGGHTGGFG
                     ADPAEYEQRVVGFFDRTLLRAADGR"
BASE COUNT          408 a         1307 c         1281 g          379 t
ORIGIN      
        1 atcggggcgc tcctcgacga cccagccgtc gggagacgcg gtgacgccgc ggcggtcgac
       61 gcggaggcga agcggccggc cgcggaaccg aagcgagagc tcgagcgcgt tccactcgcg
      121 cggaaggcgc gggtcgacgt gaaggcgccc gccgcggggg cggacgcccg cgaagccgtg
      181 aacgagcgcc tgccagagcc cgcccatcgt cgcgaggtgg agcccgccag cggtgctccc
      241 ggtcaggtcg tcgagatcga gccgcgccgc gatccggagc gcgtcgagcg ccggccggta
      301 gcgcccggcc cgcgcgagca gcgacgcgtg gatggccggc gacagcgagc ttccgtgcgc
      361 ggtgcgcggc tcgtagaagt cgaggttcgg cacgagcgag ccggccggaa cctcctcggg
      421 cagcaggtgg tggagcatga gcacgtccgc ctgcttcagc acctgcgcgc ccgccgtccg
      481 ctcggcgccg agcaggaggt cggcggcgat cggccgcttg ggcgcgacct cggcgatcac
      541 gagcggctcg aggtcggaga agccggcgaa ctgctcgtag atcccgctcg cgcggtcgta
      601 gccgtccacg agcgcgtctg cgacctcgag ccacgtcgcc cgctcgcccg cgtcgggtcc
      661 gggaagcgcc gcggcgcgcc ggaggttcca gcgggcgagg acgttcgtga acgcgttgtc
      721 gtcgacgggc tcgtggtact cgtcgggccc gatgacgccg tagatgtgcg cgcgcccgtc
      781 cgcgccgcgc cgcacgcgcg aggcccagta gcgggccgtc tcgacgagga gctcgcgccc
      841 gggcccggag gcgaactcct cgtcgcccgt ccagtcgagg tagcaggcga cggcccaggc
      901 gacgtcgccg acgatgtgct cctcgagctc tcccgtgcgg atgcggacga gctcgccggt
      961 cgcgagccgg accgacggcg gcgtcacgtc gcgcccgtcg gcggcggact cccacgcgaa
     1021 gcgcgcgccc gcccgtccga gcgcgcacgc cgcctcgcgc gccgccggca gcctgcggac
     1081 gcggtactcg agcatcgcgc gcgcggccgg gggatgggtt gccgcgagaa acgggaggac
     1141 gaagacgtcg ctgtcccaga agacgtgccc gctgtacgcc ggcccggtca ggccgcgcgc
     1201 gccgacggcc gcctcgccct cgtcgccgac ggaggccatc aggtggaaga gcgcgaagcg
     1261 gatcgccttc tgcagcgcgg ggtcgccctc gaggacgacg tcggcgcccg cccaccggct
     1321 cgcccacgcg tggcgctgct cgcgcaggag ccgctcgaag ccggccgcct cggccgaccc
     1381 gaccgccgtc cgcgcagcct cctcgtccag gtcgtaggcc gcgatgcgct cgacgatggc
     1441 cccggcttgc cgctcgcgca ccgcgacgct gaccgcgccg cgcacgtcgt cgcccgccgg
     1501 cggcacgacc tcccggccgt ccgcctccgc ccgcagcgcg accgtgccgg gccgcgcgag
     1561 cgaagagaag cgaagcgacg cgcccggtcc ttcctcgcgg agcaggcccg tcgccaggtc
     1621 gagccgccgg gtgagcgacg gcgccggtgg gccgggagcg gggaggagcc ggctccagtc
     1681 cggggcgcgc gccagctcgg tccgcgagcc gtccccggtg tagacgccgg agaggagcac
     1741 ggcccgctcg accgacgggt cgtcgaagag cggcgcgccg cgcgtcccga gccgcccgtc
     1801 ggcgagcgtc aggagcgact cgtgcacgcg ctccagcgcg gggtcgaggc cctcgacggc
     1861 gagcgtccac tcggagcccc cgtcgagctc cgggacgtcg ccgcggcggc ggcgctcgag
     1921 ctggtcggcc aggatgcgca ggaacacctc cgggccgccg ccgagcgcaa gcacgtcggc
     1981 cggcgcgccg ctcggctcgg cgccgaccgt gagcgcagtg gccgccgcgg cctcgggcac
     2041 gagcaggaga gagtcgctgc cgggcagccc gccgagcggg ccgaactcgt cgcccgcgac
     2101 gagcgtgagg ccgggcccga caccgcgccg ggcgagctcc gcgaccgccc agcgtgccgc
     2161 gtccgctttg tccgtgagcc cgacctccac gtgcttcgcg tcgctcgtga cgcgcgggtc
     2221 ctggatgccc gccgagcggg cgatcgtcgt cgcgatctcg accgcgtcgc cgagagtgat
     2281 gccggcggcg ccgagccgct cctgcacggc cttcagcagc tccgcgatcc gggccttcgg
     2341 cgggtcggcc cactcgggct ccgggatcag gtcgatcttg cgccggttga gccgcttgga
     2401 gacgatcgcc gccgcgacgc cgcggcggcc gagctcctcg accgtcgccg ccgcggccgc
     2461 gtcgagcgcg gcctcctcct cgggcgtcgc ctcgcgccgg tcgagcagct cgacgccgtc
     2521 ctcgcccgcg acgaacacct ccgagccgcg gttgacgcac aggtagagcc gcccgggccc
     2581 ggccgggcgc gcgccgagct gcccgtcgac gttgccgacg tgcgtcccgg tgatgacgac
     2641 gaggtcgagc ccgcgctcgc agagctcctc gacgagggcg cggaggtccg cggcgtcggc
     2701 cgcacggtcg gggaccgcgg ttccgtccca gtcgaacacg atcgcctcga agcgccggtc
     2761 gagcgccgcg ggcagcggct cgtcggcgcc acggcggcgg gtgcgctcgg tggtgctcac
     2821 cggccgtccg ccgcgcggag gagcgtgcga tcgaagaagc cgaccacgcg ctgttcgtac
     2881 tcggccggat cggccccgaa gccgcccgtg tgtccgcctg tctccacgcg ccagacctcc
     2941 ttcggctcgc cggccgcctc gaagaactcc ggggtcaggt cctcgccgcc ctgcccctcc
     3001 gccgcgtaga cgaggaagac ggcacgcggc gcgatccggg cgatcgcgtc gtcgagcgcg
     3061 ggcggcggcg ccgttccgct cagcacagcg agggcggcgg tctgcgtcag ctgctgcggc
     3121 tgggcgagcc agccgcgcgg gccgtagagc cgcgcctcgc ggatggagcg cacgccggcg
     3181 cccgcggaga cgacggcgcg caggccgtcg tctccggcgg ccgcctcgag catcatctcg
     3241 ccgccgaccg agaacccgat cccgccgatg cggccgtcgt ccacgtccgg ccgctcgtcc
     3301 agccatgcga ccgcggcgtc gatgtccggc gtcgaccccc agccgaacat gttcggactt
     3361 ccctcgctgc cgtcg
//
LOCUS       DASTFC010000186         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1058648,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000186 DASTFC010000000
VERSION     DASTFC010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058648"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFO88_05625"
     CDS             complement(<1..362)
                     /locus_tag="VFO88_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HET9545036.1"
                     /translation="MLTVDITLPDIEELHKLVVEGQEKGFLTYDEIVHGLDEVELTKE
                     QIEDFYTYLIDHGIELHEGEEHKTPPHEQPALAEDEKSAPKLDLTVEPSLDSLRLYLR
                     EIGKVPLLTADQEIYLAKR"
     gene            complement(460..1365)
                     /locus_tag="VFO88_05630"
     CDS             complement(460..1365)
                     /locus_tag="VFO88_05630"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET9545037.1"
                     /translation="MRTDPISVVVERGGVVESVHTAHAAVVRDGELVGAVGDAGLVTF
                     MRSAAKPFQALALAEEEPGLPPEELAIACASHEATPEQLRLVASLLARAGAREDELAC
                     GEEHGSKSGHNCSGKHAGMLLRVRRHGWPPDYVEAGHPLQAELLATVAAAAGVPPGEI
                     RTAVDGCGVPTFALSLEAMAQAFARLARRELPGSGPVVAAIVAHPELVGGPQAADTAI
                     MRARPGAVAKRGAEGVLCAALPDGTGVAVKVADGANRAAGPALAALLGLQDLAERAVF
                     NSRCEEVGRLFPRWKNSGSLFPKAM"
     gene            1380..2207
                     /locus_tag="VFO88_05635"
     CDS             1380..2207
                     /locus_tag="VFO88_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9545038.1"
                     /translation="MPELPEVEAWRRSLDERLRGATIAGAGPAHIATLKTFDPPLAAL
                     EGLTIAGVERRGKRFLVPLEGEDLVVLVHLMSAGRLKLVEPGAKRPGQPAFRLGFDDG
                     GELVLTETGRKKRAGVWLLTREGAEAENAHLGPEADAIGEVELAAILHAESRRLHSFL
                     RDQRALAGIGRAWANEILHTARLSPYALSGELSSEEVTRLHEAIRSELARGLALRERG
                     APDSKVYRVHKRLGEPCHVCGTPLAQVDFEEHTIFYCPECQTGGRVLKDRRMSRLLR"
BASE COUNT          315 a          832 c          838 g          335 t
ORIGIN      
        1 cgcttggcga ggtagatctc ctggtcggcg gtgaggagcg gaaccttccc gatctcgcgc
       61 aggtagaggc gcagcgagtc gagggacggc tcgacggtca ggtcgagctt cggggcgctc
      121 ttctcgtcct cggcgagcgc gggctgctcg tgcggcgggg tcttgtgctc ctcgccttcg
      181 tgcagctcga tgccgtggtc gatgaggtag gtgtagaagt cttcgatctg ctccttggtc
      241 agctccacct cgtcgagccc gtggacgatc tcgtcatagg tgaggaaccc cttctcctgt
      301 ccttcgacga cgagcttgtg cagctcttcg atgtcgggga gcgtgatgtc gaccgtgagc
      361 attcgacctc gccttcgtcg cttacgtggg ggtttgcaac gaaaagagcc ggcgagctca
      421 ctcccctctc cggctcccta gcctgcagcg gcggtgattc tacatagcct tggggaaaag
      481 tgaacccgaa ttcttccatc tggggaaaag tcgccccact tcttcacagc ggctgttgaa
      541 aaccgcccgt tctgccagat cctggaggcc gagcagggcc gccaacgcgg gaccggccgc
      601 gcggtttgcg ccgtccgcca ccttgacggc gacaccggtg ccgtccggaa gggccgcgca
      661 gaggacgccc tcggcccccc gcttcgcgac cgcgcccggg cgcgcgcgca tgatcgccgt
      721 gtcggccgcc tgcggcccgc cgacgagctc ggggtgcgcg actatcgccg ccacgacggg
      781 ccccgagccg ggcagctcgc ggcgcgcgag ccgcgcgaag gcctgcgcca tcgcctcgag
      841 cgagagcgcg aacgtcggga cgccgcagcc gtcgacggcg gtccggatct cgcccggcgg
      901 gacgcccgcg gcggcggcga ccgtagcgag cagctcggcc tggagcggat ggccggcctc
      961 cacgtagtcg ggcggccagc cgtgccgccg gacgcgtagg agcatcccgg cgtgcttgcc
     1021 cgagcagttg tgaccgctct tcgagccgtg ctcctcgccg cacgcgagct cgtcctcccg
     1081 cgcgcccgcg cgcgcgagga gggaggcgac gagccggagc tgctcgggcg tcgcctcgtg
     1141 cgaggcgcag gcgatcgcga gctcctctgg gggcaggcca ggctcctcct cggcgagcgc
     1201 gagcgcctgg aacggcttcg cggccgagcg catgaaggtg acgagcccgg cgtcgccgac
     1261 cgcgcccacg agctccccgt cgcggacgac cgccgcgtgc gccgtgtgca cggactcgac
     1321 cacgccgccc cgctcgacaa ctacgctgat cgggtccgtt cgcacgagga cacaagcgta
     1381 tgccagagct ccccgaggtc gaagcgtggc gccgctcgct cgacgagcgg ttgcgcggtg
     1441 cgacgatcgc gggcgccggc ccggcgcaca tcgcgacgct gaagaccttc gacccgccgc
     1501 tcgccgcgct cgagggactg acgatcgccg gcgtcgagcg gcgcggcaag cggttcctcg
     1561 tcccgctcga gggcgaggat ctcgtcgtcc tcgtccacct gatgagcgcc gggcgcctga
     1621 agctcgtgga gccgggcgcc aagcggccgg gacagccggc gttccggctg gggttcgacg
     1681 acggcggcga gctcgtccta acggagaccg gccggaagaa gcgggccggg gtctggctcc
     1741 tgacgcgcga gggcgcggag gccgagaacg cgcacctcgg ccctgaggcg gacgcgatcg
     1801 gcgaggtcga gctcgccgcg atcctgcacg ccgagtcgcg gcgcctgcac agcttcctcc
     1861 gcgaccagcg cgcgctcgcc gggatcggcc gcgcctgggc gaacgagatc ctccacaccg
     1921 ccaggctctc gccgtacgcg ctctcgggcg agctctcgtc ggaggaggtg acgcgcctgc
     1981 acgaggcgat ccggtcggag ctcgcgcgcg gcctcgccct ccgcgagcgc ggcgcgccgg
     2041 actcgaaggt ctaccgcgtg cacaagcgcc tgggcgagcc gtgccacgtc tgcggcacgc
     2101 cgctcgcgca ggtcgacttc gaggagcaca cgatcttcta ctgcccggag tgccagaccg
     2161 gcggacgcgt cctcaaggac cgccggatgt ctcggctgct ccgctagcgg tcctacttcc
     2221 cgccgggcgc gtgctgtcgg gcgaggtcga gggcgacgac gagcagcagc gtcgtccccc
     2281 agacgttcac ggggtcgaag cggatcggct ccgagtcgcc
//
LOCUS       DASTFC010000187         5463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_794945,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000187 DASTFC010000000
VERSION     DASTFC010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794945"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFO88_05640"
     CDS             <1..315
                     /locus_tag="VFO88_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9545039.1"
                     /translation="ELDGRALVRLAEAGDADARGVIDTIGRRLGAGVAGYVNVFNPEV
                     VVIGGGFGAAGDFLLDPVREVVAEQALPTARDEVRIVPAELGPDAGLVGAGLVAFDLL
                     AG"
     gene            315..1145
                     /gene="rsmI"
                     /locus_tag="VFO88_05645"
     CDS             315..1145
                     /gene="rsmI"
                     /locus_tag="VFO88_05645"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9545040.1"
                     /translation="MPLVVCPTPIGNLEDVTLRALRELGEADAVLCEDTRRTRVLLDR
                     HGIRARALVSYHRLNEEARIAELLPRLEAGERLALASDAGLPGVNDPGARLVAAALDR
                     GLEVTVLPGPSAVETALVASGLIRERFTFLGYLPRRPTELERLWEELAGWTWPAVAFE
                     SPRRLPASLASLAAAAPDRPVAVCRELTKRFEEVVRGSAAEVAARFAEPPKGEITLVI
                     GAASPRSADDADAVAAVAELVAAGLPRRKAADLVARLTGAPRNALYRGSLDEPMRRRT
                     "
     gene            complement(1126..1401)
                     /locus_tag="VFO88_05650"
     CDS             complement(1126..1401)
                     /locus_tag="VFO88_05650"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET9545041.1"
                     /translation="MAANGNKRDGARTGIVRHIDELGRIVIPSEIRKRFGFGVKDPVE
                     ISVRGETILLTRPQTACVFCGRGDRLSEYRGRCVCHSCIGELKSVAA"
     gene            1481..3262
                     /gene="metG"
                     /locus_tag="VFO88_05655"
     CDS             1481..3262
                     /gene="metG"
                     /locus_tag="VFO88_05655"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9545042.1"
                     /translation="MPRRILVAVAWPYANGPRHIGHVAGFGVPADIFARYHRLRGDDV
                     LMVSGTDEHGTPIMVAADREGVSPRELADRMSAVIRQDLQALGLSYDLFTRTTTKNHH
                     RVTQDLFRTLYDKGFVLKQATTGAFSATTGHTLPDRYIEGTCPICGYGEARGDQCDNC
                     GNQLDPADLVDPRSKIDGTAPVFRETEHFFLDLPAFKERLAEWIGAQKQWRPNVRKFS
                     LGLVDDLKPRPITRDLDWGVRIPLPELEERDDKRIYVWFDAVIGYLSAAVEWAHVTGQ
                     PDAWRDWWQDPDARHAYFMGKDNIVFHSVIWPSMLMGYGAGGEVGGGERELELPDDIV
                     ATEFLTMEGKKFSSSRGVVIYVRDFLSRYDPDPLRYFLTIAGPETQDTDFTWAEFVRR
                     NNDELVATWGNLVNRTVTNAHRNFGAVPSPGELEEADREILAALEGGFTTVGDLLERA
                     SFRAALAETMALASRVNGYVSDAAPWALVKEDRERAGTVLYVALRAVDSLKTLFAPFL
                     PFSSQAVHELLGYEGWLAGPLELRQVGEEDGDPHEVLTGDYAAWTGSWTPSELPPGQE
                     LREPRPLFRKLDPDVVVPEELRRMEEQ"
     gene            3259..4002
                     /locus_tag="VFO88_05660"
     CDS             3259..4002
                     /locus_tag="VFO88_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9545043.1"
                     /translation="MIDTHAHLDHEDDPAAVLARARAAGVARVIAVGSGLASCAATLE
                     LAEREDGVFAALGFHPHQAGEESDLGALRELLSHPRAVAVGETGLDWFRDYAPRDRQE
                     ALFRAQVALAAEVGKALVVHTRAADEDTVAVLRDLAGDATVVLHCFSSWRLLEPALAH
                     GWYCSFAGNVTYPKAGELRAAAAEVPRDRLLAETDSPYLAPQPVRGKRNEPAHVVHTL
                     AVLAEARGEDPADLEAVIEENAARAFGLP"
     gene            4010..4789
                     /gene="rsmA"
                     /locus_tag="VFO88_05665"
     CDS             4010..4789
                     /gene="rsmA"
                     /locus_tag="VFO88_05665"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9545044.1"
                     /translation="MSRPAARKELGQHFLVDENIVRVAGRLAELGPEDVVLEIGPGLG
                     VLTAFLADRVRLVHAVELDRSLEPALRERLGARTNVELVFGDALRVPLNELDPPPGKL
                     VANLPYNVATPLVVESLDGLPTVERWCVMVQREVADRFFAEPGTRAYGAVTVLLRLTA
                     ERTGFHPVPPSVFRPQPNVDSALVAFRRTRTWGEEYAPLKRVVQAAFAHRRKTLANSL
                     ALAGLDRARADEAVAAAGLDRAVRAEALEPETFLALAERLA"
     gene            4786..>5463
                     /locus_tag="VFO88_05670"
     CDS             4786..>5463
                     /locus_tag="VFO88_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545045.1"
                     /translation="MTELLAHAKVNLALVVGEKRADGLHPVANVLQRIDLADRLVLEP
                     AAALAVEGFPEDTLVRRALERLAAAAGVEPAWRVRIDKQIPVAAGLGGGSSDAAAALA
                     AANETLAGALSGDALLELAAGLGADVPFFLRPGPQLAEGAGERLTPLDLPLGYRVVVA
                     LPHGAEKRSTADVYRRFDELGGGPGFDDRRAALLPALAARDLAGLPPNDLAPAAGPAP
                     LVDELRAA"
BASE COUNT          688 a         2025 c         2023 g          727 t
ORIGIN      
        1 gagctcgacg ggcgcgcgct cgtccggctg gcggaggccg gagacgcgga tgcgcgcggc
       61 gtgatcgaca cgatcgggcg gcggctcggc gccggcgtcg cgggctacgt gaacgtcttc
      121 aacccggagg tcgtcgtgat cggcggcggc ttcggagcgg ccggcgactt cctcctcgac
      181 ccggtgcgcg aggtcgtcgc cgagcaggcg ctcccgacgg cgcgcgacga ggtgcggatc
      241 gtcccggccg agctcggccc ggacgcgggg ctcgtcgggg cggggctcgt cgcgttcgac
      301 ctcctcgccg gctgatgccg ctcgtcgtct gcccgacccc gatcggcaac ctcgaggacg
      361 tcacgctccg agccctccgc gagctcggcg aggccgacgc cgtcctctgc gaggacacgc
      421 ggcgcacgcg ggtcctgctc gaccggcacg ggatccgtgc acgggcgctc gtctcgtacc
      481 accggctgaa cgaggaggcg cggatcgcgg agctgctccc gcggctcgag gcgggtgagc
      541 ggctggcgct cgcaagcgac gcggggctcc cgggcgtgaa cgaccccggt gcgcgcctcg
      601 tcgccgccgc gctcgaccgc gggctcgagg tgacggtgct gccgggcccg tcggccgtcg
      661 agacggcgct cgtcgcgagc gggcttatcc gcgagcggtt cacgttcctc ggctacctcc
      721 cgcgccggcc gacagagcta gagcggctct gggaggagct cgccggctgg acgtggccgg
      781 cggtcgcgtt cgagtcgccg cgccgcctcc ccgcgagcct cgcgagcctg gccgcggcgg
      841 cgccggaccg cccggtcgcg gtctgccgcg agctgacgaa gcgcttcgag gaggtcgtcc
      901 gcgggagcgc ggccgaggtc gccgcccggt tcgccgagcc gccgaagggc gagatcaccc
      961 tcgtgatcgg cgcggcctca ccgcggagcg cggacgacgc ggacgccgtc gccgcggtcg
     1021 ccgagctcgt ggccgcgggg ttgccccgcc gcaaggcggc cgatctcgtc gcgcgcctga
     1081 cgggcgcgcc gcggaacgcg ctctaccgtg gctccctgga cgagcctatg cggcgacgga
     1141 cttgagctcg ccgatgcacg agtgacacac gcaccgtccc cggtactcgg agagccgatc
     1201 gccgcggccg cagaagacgc acgccgtctg cggtcgggtg aggaggatcg tctcgccccg
     1261 gacggagatc tccacgggat ccttgacccc gaacccgaac cgcttgcgga tctcgctcgg
     1321 gatcacgatc cgcccgagct cgtcgatgtg cctgacgatg cccgtccggg caccgtcgcg
     1381 cttgttgccg tttgctgcca tacggcgacg ctttcccagg ggctggaagg ggaaacccca
     1441 tgcaagggcc gcgtaaggac tgcgtaggat cagcctctcg atgccccgcc gcatcctcgt
     1501 cgccgtcgcc tggccgtacg ccaacggccc gcggcacatc ggccacgtgg ccggcttcgg
     1561 cgttccggcg gacatcttcg cccgctacca ccgtctgcgg ggcgacgacg tcctcatggt
     1621 cagcgggacg gacgagcacg ggacgccgat catggtcgcc gccgatcgcg agggcgtctc
     1681 cccgcgcgag ctcgccgacc ggatgagcgc ggtgatccgg caggaccttc aggcgctcgg
     1741 gctctcgtac gacctcttca cgcgcacgac gacgaagaac caccaccggg tcacgcagga
     1801 tctcttcagg acgctctacg acaaggggtt cgtcctgaag caggcgacga ccggcgcgtt
     1861 ctcggcgacg accggccaca cgctccccga ccgctacatc gagggcacgt gcccgatctg
     1921 cggctacggc gaggcgcgcg gcgaccagtg cgacaactgc gggaaccagc tcgaccccgc
     1981 cgacctcgtc gacccgcgct cgaagatcga cgggacggcg ccggtcttcc gcgagaccga
     2041 gcacttcttc ctcgacctgc cggccttcaa ggagcggctc gcggagtgga tcggcgcgca
     2101 gaagcagtgg cgtccgaacg tgcggaagtt ctcgctcggc ctcgtcgacg atctgaagcc
     2161 gaggccgatc acgcgcgacc tcgactgggg cgtccgcatc ccgctcccgg agctcgagga
     2221 gcgcgacgac aagcggatct acgtctggtt cgacgcggtg atcggctacc tctcggccgc
     2281 ggtcgagtgg gcgcacgtga cggggcagcc ggacgcctgg cgggactggt ggcaggaccc
     2341 ggacgcgcga cacgcgtact tcatgggcaa ggacaacatc gtcttccact cggtcatctg
     2401 gccgtcgatg ctgatgggct acggcgccgg gggagaggtg ggcggcggcg agcgcgagct
     2461 cgagctgccg gacgacatcg tcgcgaccga gttcctcacg atggagggga agaagttctc
     2521 ctcgtcgcgc ggggtcgtca tctacgtgcg cgacttcctc tcgcgctacg accccgatcc
     2581 gctgcgctac ttcctgacga tcgcgggccc cgagacgcag gacacggact tcacctgggc
     2641 cgagttcgtg cggcgcaaca acgacgagct cgtcgcgacg tgggggaacc tcgtgaaccg
     2701 gacggtcacg aacgcccacc ggaacttcgg cgcggtgccc tcgccgggag agctcgagga
     2761 ggccgaccgc gagatcctcg cggcgctcga gggcgggttc acgaccgtgg gcgacctgct
     2821 cgagcgggcg agcttccgcg ccgcgctcgc ggagacgatg gcgctcgcct cgcgcgtgaa
     2881 cggctacgtg tcggacgcgg cgccgtgggc gctcgtgaag gaggaccgcg agcgggcggg
     2941 cacggtgctc tacgtcgcgc tgcgggcggt cgactcgctg aagacgctct tcgccccgtt
     3001 tctcccgttc tcgtcgcagg ccgtgcacga gctgctcggc tacgaggggt ggctcgcggg
     3061 cccgctcgag ctgcggcagg tgggcgagga ggacggcgac ccgcacgaag tgctcacggg
     3121 cgactacgcc gcgtggaccg gctcgtggac gccaagcgag ctgccccccg ggcaggagct
     3181 ccgcgagccg cggccgctct tccggaagct cgacccggac gtcgtcgtgc cggaggagct
     3241 tcgccgcatg gaagagcagt gatcgacacg cacgcgcacc tcgaccacga ggacgacccg
     3301 gccgcggtgc tcgcgcgcgc gcgtgcggcg ggggtcgcgc gcgtgatcgc cgtggggtca
     3361 ggtcttgcgt cgtgcgcggc gacgctcgag ctcgcggagc gcgaggacgg cgtcttcgcg
     3421 gcgctcggct tccacccgca ccaggcgggc gaggagtccg acctcggcgc gctccgggag
     3481 ctgctctcgc acccgcgggc ggtcgcggtg ggtgagacgg ggctcgactg gttccgcgac
     3541 tacgcgccgc gcgaccggca ggaggcgctc ttccgcgcgc aggtcgcgct cgcggccgag
     3601 gtcggcaagg cgctcgtcgt gcacacgcgc gcggcggacg aggacacggt cgcggtgctg
     3661 cgcgacctcg ccggtgacgc gacggtcgtg ctccactgct tctcctcgtg gcggctgctc
     3721 gagccggcgc tcgcgcacgg ctggtactgc tcgttcgccg gcaacgtgac gtacccgaag
     3781 gcgggcgagc tgcgcgccgc cgcggccgag gttccgcgcg accggctgct cgccgagacc
     3841 gacagcccgt atctcgcgcc gcaacccgtg cgcgggaagc ggaacgagcc ggcgcacgtc
     3901 gtccacacgc tcgcagtcct cgccgaggcg cgcggcgagg atcccgccga cctcgaggcc
     3961 gtgatcgagg agaacgccgc gcgcgcgttc ggcctgccgt gagggcggcg tgagccggcc
     4021 ggcggcgaga aaggagctcg gccagcactt cctggtggac gagaacatcg tccgtgtcgc
     4081 cgggcggctc gccgagctcg ggccggagga cgtcgtcctc gagatcgggc ccgggctcgg
     4141 ggtgctgacc gcgtttctcg ccgatcgcgt ccgcctcgtc catgccgtcg agctcgaccg
     4201 ctcgctcgag ccggccttgc gcgagcggct cggcgcgcgc acgaacgtcg agctcgtctt
     4261 cggcgacgcg ctccgcgtcc cgctcaacga gctcgacccg ccgccgggga agctcgtggc
     4321 gaacctcccg tacaacgtcg cgacgccgct cgtggtcgag agcctcgacg ggctcccgac
     4381 cgtggagcgc tggtgcgtca tggtgcagcg ggaggtggcc gaccgcttct tcgccgagcc
     4441 ggggacgcgg gcgtacgggg ccgtgaccgt cctgctccgg ctgaccgccg agcggaccgg
     4501 cttccatccc gtcccgccga gcgtcttccg gccgcagccg aacgtcgact cggccctcgt
     4561 cgcgttccgg cgcacgcgca cgtgggggga ggagtacgcg ccgctcaagc gcgtcgtcca
     4621 ggcggcgttc gcgcaccgcc ggaagacgct cgccaactcg ctcgcgctcg ccgggctcga
     4681 ccgtgcgcgg gccgacgagg cggtcgccgc ggccgggctc gaccgcgcag tgcgcgcgga
     4741 ggcgctcgag ccggagacgt tcctcgccct cgcggagcgt ctcgcgtgac cgagctcctc
     4801 gcgcacgcga aggtgaacct cgcgctcgtc gtcggcgaga agcgcgcgga cgggctgcac
     4861 ccggtcgcga acgtcctcca gcggatcgac ctcgccgacc ggctcgtgct cgagccggcc
     4921 gccgcgctcg ccgtcgaggg attcccggag gacacgctcg tccgccgcgc gctcgagcgg
     4981 ctcgcagccg cggccggcgt cgagccggcg tggcgggtca ggatcgacaa gcagatcccc
     5041 gtcgcggccg gcctcggcgg cggcagctcc gacgccgccg ccgcgctcgc ggccgcgaac
     5101 gagacgctcg ccggggcgct ctccggcgac gccctcctcg agctcgcggc cgggctcggc
     5161 gccgacgtcc ccttcttcct ccgcccgggc ccgcagctcg cggagggggc cggggagcgg
     5221 ctgacccccc tcgacctgcc gctcggctac cgcgtcgtcg tcgcgcttcc gcacggggcg
     5281 gagaagcgct cgaccgcgga cgtctaccgc cgcttcgacg agcttggcgg cgggcccggg
     5341 ttcgacgacc gccgggccgc gctcctcccc gcgctcgccg cccgcgacct cgccggcctc
     5401 ccgcccaacg acctggcccc cgccgcgggg cccgcgccgc tcgtcgacga gctgcgcgcc
     5461 gcg
//
LOCUS       DASTFC010000188         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1256266,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000188 DASTFC010000000
VERSION     DASTFC010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256266"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFO88_05675"
     CDS             complement(<1..550)
                     /locus_tag="VFO88_05675"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545046.1"
                     /translation="MADLFSDAAEARLPERAPLAARLRPARLEDFVGQEGVVGPGTAL
                     AFAIAEDRMTSAIFYGPPGSGKTTLARIVAGATGAAFEELSAVSASVSDVRGVLAAAL
                     ERLGATGQRTILFLDEIHRFNKAQQDALLPAVENWLLTLIGATTENPYFEVNSALLSR
                     AQIYELEPLADEHVAEIVRRGAA"
     gene            586..1383
                     /locus_tag="VFO88_05680"
     CDS             586..1383
                     /locus_tag="VFO88_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9545047.1"
                     /translation="MAFEELKERQRVMWGSGPFERIEAIIADMHERVVQRLRPQPDER
                     WLDVGCGTGGVAALAARAGADVTGIDLAPALVETARGRAAADGIPCRYEVGDAEQLPY
                     EDASFDVVASTVGAMFAPDQGAVASEFARVLRPGGRLAFTAWRPEGGGGDLFRLLAPF
                     QPPPPEGVPSPLEWGREEHVRALLGDAFALEIEEHDSPYTPASGEEAWELFATAFGPL
                     RTLVDALPPERVDELRRVFVAYHEEQRGDDGAIRVSRRYLLVLGTRR"
     gene            1380..>2501
                     /locus_tag="VFO88_05685"
     CDS             1380..>2501
                     /locus_tag="VFO88_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9545048.1"
                     /translation="MSTPALRDEATELLQELVRLDTVNPPGNETAAAELLRDYLAESG
                     VEGELLAREPDRANLVARIPGTGGGKRLLLVGHTDTVLADPAEWTVDPWSGELRDGCV
                     WGRGALDMKSQVAANAVAFARLAREGFRPAGDLVFAATADEEVGDADVGLSWLCREHP
                     EAVRAEYSVTEGGGDRALVAGRPVYLCAVAEKASAPFRLRVRGRSGHASMPGIADNAL
                     VKAARLVERLGGHRPERRILPETHAFLTTLFGRVPGPEELDELASGLDEPLAGLVEPM
                     LGVSVSPTMIEASRKRNVIPGTCEVVCDCRLLPGQSRAEAEEELRRCLGDGDYELEWI
                     ERVGGSRSSSATPLFEAITDWVAREEPGAAVLPVILPGFT"
BASE COUNT          304 a          912 c          960 g          325 t
ORIGIN      
        1 cggccgcgcc gcggcggacg atctccgcca cgtgctcgtc cgcgaggggc tcgagctcgt
       61 agatctgggc gcgtgagagg agcgccgagt tgacctcgaa gtacgggttc tcggtcgtcg
      121 ctccgatgag cgtgaggagc cagttctcga ccgccgggag cagcgcgtcc tgctgcgcct
      181 tgttgaaccg gtggatctcg tcgaggaaga ggatcgtccg ctggcccgtc gcgccgagcc
      241 gctcgagcgc cgccgcgagg acgccgcgca cgtccgagac cgacgccgag accgccgaca
      301 gctcctcgaa cgcggcgccg gtcgccccgg cgacgatccg cgcgagggtg gtcttcccgc
      361 ttcccggcgg cccgtagaag atcgcggagg tcatccggtc ctccgcgatc gcgaaggcga
      421 gcgcggtgcc gggcccgacg acgccctcct gtccgacgaa gtcctcgagc cgcgcgggcc
      481 gcaggcgggc cgcgagcggc gcgcgctcgg gcagccgcgc ctcggccgcg tcggagaaga
      541 gatcggccat ggccgaagta tgctcaggcc gaaaggagga cgagcatggc cttcgaggag
      601 ctgaaggagc ggcagcgcgt catgtggggc tcggggccgt tcgagcggat cgaggcgatc
      661 atcgccgaca tgcacgagcg ggtcgtgcag cgcctccgcc cgcagccgga cgagcgctgg
      721 ctcgacgtcg ggtgcggcac ggggggcgtc gccgcgctcg cggcgcgggc cggggcggac
      781 gtgacgggca tcgatctcgc cccggcgctc gtcgagaccg cacgcggccg cgcggccgcg
      841 gacggcatcc catgccgcta cgaggtcggc gacgcggagc agctgcccta cgaggacgcg
      901 tcgttcgacg tcgtcgcctc gacggtcggc gcgatgttcg ctcccgacca gggcgcggtc
      961 gcgtcggagt tcgcccgcgt gctgcggccc ggcggccgcc tggcgttcac ggcctggcgc
     1021 ccggagggcg gcggcggcga cctcttccgg ctcctggcgc ccttccagcc gccgccgccg
     1081 gagggcgtgc cgagcccgct cgagtggggc cgcgaggagc acgttcgcgc gctcctgggt
     1141 gacgccttcg cgctcgagat cgaggagcac gactcgccgt acacgccggc ctcgggggag
     1201 gaggcgtggg agctcttcgc gactgccttc gggcccctgc ggacgctcgt ggacgcgctc
     1261 ccgccggagc gggtcgacga gctgcgccgg gtgttcgtcg cctaccacga ggagcagcgc
     1321 ggcgacgacg gtgcgatccg cgtctcgcgc cggtacctgc tcgtcctcgg cacccgccgg
     1381 tgagcacgcc cgcgctccgc gacgaggcga cggagctgtt gcaggagctc gtccggctcg
     1441 acaccgtcaa ccccccggga aacgagacgg ccgcggcgga gctccttcgc gactatctcg
     1501 cggagtcggg agtcgagggc gaactcctcg cgcgggagcc cgaccgggcc aacctcgtcg
     1561 cccgcatccc ggggacgggc ggcggcaagc ggctcctgct cgtcggccac acggacaccg
     1621 tgctcgccga cccggccgag tggacggtcg acccgtggtc gggcgagctc cgcgacggct
     1681 gcgtctgggg tcgcggcgcg ctcgacatga agagccaggt ggcggcgaac gcggtcgcat
     1741 tcgcgcggct cgcgcgggag ggcttccgcc cggcgggcga cctcgtcttc gccgcgaccg
     1801 ccgacgagga ggtcggcgac gcggacgtcg ggctctcctg gctctgccgc gagcacccgg
     1861 aggcggtgcg cgcggagtac agcgtcaccg agggcggcgg cgaccgcgcg ctcgtcgccg
     1921 gtcggccggt ctatctctgt gcggtcgccg agaaggcgag cgctcccttc cgcctgcgcg
     1981 ttcgcggccg cagcggccac gcgtcgatgc ccggcatcgc cgacaacgcg ctcgtcaagg
     2041 cggcgcggct cgtcgagcgg ctgggcgggc accgccccga gcgccgcatc ctgcccgaga
     2101 cgcacgcctt cctgacgacg ctcttcggcc gcgtcccggg cccggaggag ctcgacgagc
     2161 ttgcgtcggg tctcgacgag ccgctcgcgg gcctcgtcga gccgatgctc ggcgtgagcg
     2221 tctcgccgac gatgatcgag gcctcccgca agcgcaacgt gatcccgggg acgtgcgagg
     2281 tcgtctgcga ctgccggctc ctccccggtc agagccgggc ggaggcggag gaggagctcc
     2341 gcaggtgcct cggcgacggt gactacgagc tcgagtggat cgagcgcgtc ggcggctcgc
     2401 gctcgtcctc ggcgacgccg ctcttcgagg cgatcacgga ctgggtcgcg cgcgaggagc
     2461 ccggcgcggc cgtcctcccc gtcatcctcc ccgggttcac c
//
LOCUS       DASTFC010000189         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_284093,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000189 DASTFC010000000
VERSION     DASTFC010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284093"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VFO88_05690"
     CDS             <1..1234
                     /locus_tag="VFO88_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545049.1"
                     /translation="EKDEVREPLERELAEAKERRDELAARWAEEKEALTRVAEITKRI
                     DELRMEADRAERHGELQRVAEIRYGELPALEQELAERETSDSARMVKEEVDEDDVAEV
                     VARWTGIPVSRLMEGETEKLIHMEERLHERVVGQDEAVTAVANALRRARSGLQDPDRP
                     IGSFVFLGPTGVGKTELARALAEFMFDDERAMVRLDMSEYQERHTVARLVGAPPGYVG
                     YEEGGQLTEAVRRRPYSVVLLDEIEKAHAEVFDVLLQILDDGRLTDGQGRTVDFRNAV
                     IIMTSNVRSAEEMRDRFRPEFLNRIDEIVEFQALSREQLADIVELQLARLRGRLADRA
                     IGLRLTDAAKEHVAEAGWDPAYGARPLKRALQRLVENPLALRLLEGEFEPGDTVVVDV
                     RDGELAFEKAPVAEPAAA"
     gene            1291..2040
                     /locus_tag="VFO88_05695"
     CDS             1291..2040
                     /locus_tag="VFO88_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545050.1"
                     /translation="MQTARETKEPSAANGRPPEPAAGALDRPLRLGEVLAETTRLYGA
                     RLWPAIGLGLVTAGSFLAGLLLPAAAAIALLALSLTVSWAVATRLVAGDSFGEAWAQV
                     AVRIPVLLVFTAVATVPFALALSQQLVLVIFAVGWLAGTGFAIPAAVMEKPAEPLDFF
                     HRLGWLLRRSIELARAEFLHAVGVISALVILYLFLSTILFGALRGFAEQADVAAAAIA
                     QAVLAPFFFLGLAVLYFEQRARALSSRGGRR"
     gene            2068..2355
                     /locus_tag="VFO88_05700"
     CDS             2068..2355
                     /locus_tag="VFO88_05700"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET9545051.1"
                     /translation="MPTYIMLSKLSEQGLQTLRANPERLKEVNKDVEELGARVLHQWF
                     VLGPYDFVNIVEADDAKAIARVSIAISARGSTELESFELIEVDELVNVLSA"
     gene            complement(2345..3109)
                     /locus_tag="VFO88_05705"
     CDS             complement(2345..3109)
                     /locus_tag="VFO88_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET9545052.1"
                     /translation="MLRVALLAVLLSPALVAAALARGLTPPPEPAVPPAATDPAPATT
                     ERAPAAPPAPLPPAPSPPPVAPAPPEPTWPPVRWRESRSLGLPHAGRLVGGVRLPGEG
                     EHFFTWDPVLKTSPNRPWRRWGTDRLVRVVLRVAREHRGKHPKAPRLTVGDLSRPRGG
                     DFGKRFGILGHVSHQNGLDVDVYYPRKDRREKAPRRPGQVDGRLAQDLVDRFVAAGAV
                     KVFVGPSLDLRGPRGVVEKLVHHDDHMHVRLRPLRR"
     gene            3311..4018
                     /locus_tag="VFO88_05710"
     CDS             3311..4018
                     /locus_tag="VFO88_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HET9545053.1"
                     /translation="MVGAVAAAALALAAAPGGTEAQRHVETIGHSVEGRPIRALALGD
                     RAAARRILVVGCIHGNECAGLAATRRLARMGTPPTGSVIWIVHQVNPDGARRGVRQNA
                     RGVDLNRNFPAGWRAGGRPWDGEYPGTRPFSEPETRAIRRLVRRIRPQVTIWYHQPQR
                     MVRAWGRSVAAARRYGARAGLRYRSLVWPPGTAPRWQNTRFSRQRSFVVELAPGPLSE
                     GRALRHAWAARSFVSRS"
BASE COUNT          516 a         1453 c         1550 g          521 t
ORIGIN      
        1 cgagaaggac gaggtgcgcg agccgctcga gcgcgagctg gccgaggcga aggagcgccg
       61 cgacgagctc gccgcccgct gggccgagga gaaggaggcg ctcacgcgcg tggccgagat
      121 cacgaagcgg atcgacgagc tccgcatgga ggccgaccgg gccgagcggc acggcgagct
      181 ccagcgcgtg gccgagatcc gctacggcga gctgccggcg ctcgagcagg agctcgccga
      241 gcgcgagacg agcgacagcg cccggatggt gaaggaggag gtcgacgagg acgacgtcgc
      301 cgaggtcgtc gcgcgctgga cggggatccc ggtctcgcgg ctcatggagg gcgagacgga
      361 gaagctcatc cacatggagg agcgcctgca cgagcgcgtc gtcggccagg acgaggccgt
      421 caccgccgtc gcgaacgccc tgcggcgcgc ccgctccggc ctccaggacc cggaccgccc
      481 gatcggctcg ttcgtcttcc tcggcccgac gggcgtgggg aagaccgagc tcgcccgcgc
      541 gctcgccgag ttcatgttcg acgacgagcg ggccatggtc cggctggaca tgtccgagta
      601 ccaggagcgg cacacggtcg cccggctcgt cggcgcgccg cccggctacg tcggctacga
      661 ggagggcggc cagctcaccg aggccgtccg ccgccggccc tactcggtcg tcctcctcga
      721 cgagatcgag aaggcgcacg cggaggtctt cgacgtgctc ctgcagatcc tcgacgacgg
      781 gcgcctgacc gacggccagg gccgcacggt cgacttccgc aacgcggtga tcatcatgac
      841 ctcgaacgtc cgctccgcgg aggagatgcg cgaccgcttc cggccggagt tcttgaaccg
      901 gatcgacgag atcgtcgagt ttcaggcgct ctcgcgcgag cagctcgccg acatcgtcga
      961 gctccagctc gcgcgcctgc gcggccggct cgccgaccgg gccatcggcc tgcggctcac
     1021 cgacgccgcg aaggagcacg tggccgaggc cgggtgggac ccggcgtacg gcgcgcggcc
     1081 gctcaagcgg gcgctccagc ggctcgtgga aaacccgctc gcgctgcggc tcctcgaggg
     1141 cgagttcgag ccgggcgaca cggtcgtcgt cgacgtccgc gacggcgagc tcgcgttcga
     1201 gaaggcgccc gtcgccgagc cggccgcagc ctgatccgcc gtcggcgcct cgccgaggac
     1261 acgtccgcgc cgcgaccgta cgatgcgtcg gtgcagaccg cgcgcgagac gaaggagccg
     1321 tcggcggcga acggccgccc gcccgaaccg gcggcgggcg cgctcgaccg gccgcttcgg
     1381 ctcggcgagg tcctggcgga gacgacgcgc ctctacggcg cccggctctg gccggcgatc
     1441 gggctcgggc tcgtcacggc gggcagcttc ctcgccgggc tcctcctgcc ggcggccgcc
     1501 gcgatcgcgc tcctcgcgct ctcgctcacc gtttcctggg cggtcgcgac gcggctggtc
     1561 gcgggcgact cgttcgggga ggcgtgggcg caggtggcgg tgcggatccc ggtgctgctc
     1621 gtcttcacgg ccgtcgccac ggtgccgttc gcgctcgcgc tctcgcagca gctcgtcctc
     1681 gtcatcttcg ccgtcggctg gctcgccggg acggggttcg cgatcccggc ggccgtgatg
     1741 gagaagcccg ccgagccgct cgacttcttc caccggctcg gctggctgct gcgccgctcg
     1801 atcgagctcg cgcgcgccga gttcctccac gcggtcggcg tcatctcggc gctcgtgatc
     1861 ctctacctct tcctcagcac gatcctcttc ggcgcgctgc ggggcttcgc ggagcaggca
     1921 gacgtcgctg ccgcggcgat cgcgcaggcg gtgctcgcgc cgttcttctt cctcgggctc
     1981 gccgtcctct acttcgagca gcgggcgcgg gcgctatcct cgcgcggcgg ccgacggtag
     2041 ggccgggtcg acgacgaggg aggccggatg ccgacgtaca tcatgctctc gaagctctcc
     2101 gagcagggcc tgcagacgct tcgcgcgaac ccggagcggc tgaaggaggt caacaaggac
     2161 gtggaggagc tcggcgccag ggtgctccac cagtggttcg tcctcgggcc gtacgacttc
     2221 gtgaacatcg tcgaggccga cgatgcgaag gcgatcgcgc gcgtctcgat cgcgatctcg
     2281 gcgcgcggct cgaccgagct cgagagcttc gagctgatcg aggtggacga gctcgtgaac
     2341 gttctcagcg cctgagcggc cgcagccgta cgtgcatgtg gtcgtcgtgg tggacgagct
     2401 tctcgaccac gccgcgcggg ccgcgtaggt cgaggctcgg gccgacgaag accttcacgg
     2461 cgccggcggc gacgaaccgg tcgacgagat cctgcgcgag ccgcccgtcg acctgcccgg
     2521 gacggcgcgg cgccttctcg cggcggtcct tgcgcgggta gtagacgtcc acgtcgaggc
     2581 cgttctggtg cgagacgtgg ccgaggatcc cgaagcgctt cccgaagtcc ccgccacgcg
     2641 ggcggctgag gtcgccgacc gtgaggcgcg gcgccttcgg atgcttgccc cggtgctcgc
     2701 gggcgacgcg gaggacgacg cggacgagcc ggtcggtgcc ccagcggcgc cacgggcggt
     2761 tcggcgacgt cttcaggacg ggatcccacg tgaagaagtg ctcgccctca cctgggaggc
     2821 ggacgccgcc gacgagcctg cccgcgtgcg ggaggccgag cgagcggctc tcgcgccacc
     2881 gcacgggcgg ccacgtcggc tccggcggcg cgggagcgac gggaggaggg gagggagcgg
     2941 gcggcagtgg cgcgggcggc gctgccggcg cgcgctcggt cgtggccggc gcggggtcgg
     3001 tggcggcggg tgggacggcc ggctcgggcg ggggggtgag cccgcgtgcg agcgccgccg
     3061 cgacgagcgc cggcgagagg agcaccgcga gcagggcgac ccgaagcatc ctccgagggt
     3121 acgtgccgcc tcagggcccg ggtccggccg ggcttccgcg ggaagagatc ctccccgggg
     3181 accccctctg aacacccctc acggctcacg gtagcggcga gaaccgcacg tgcgtgtgtc
     3241 gttcgtgccg tcttcgtgtc gaacctgcaa cgggacccgc acggccgccg gatcgcgtac
     3301 acataccgtg atggtcggcg ccgtcgcagc cgccgcgctc gcgctcgccg ccgcgcccgg
     3361 cggaacggaa gcgcagcgcc acgtggagac gatcgggcac tcggtcgagg gccggccgat
     3421 ccgcgcgctc gcgctgggcg accgcgcggc ggcccggcgg atcctcgtcg tcggctgcat
     3481 ccacgggaac gagtgcgccg ggctggcggc gacccggcgc ctggcccgga tgggcacgcc
     3541 gccgacagga agcgtcatct ggatcgtcca ccaggtgaat cccgacggcg cgcggcgggg
     3601 cgtgcgccag aacgcgcgcg gcgtcgacct caaccggaac ttcccggcag ggtggcgcgc
     3661 cggcgggcgg ccgtgggacg gcgagtaccc agggacgcgg ccgttctcgg agccggagac
     3721 ccgggcgatc cgccgcctcg tgcgccgcat ccggcctcag gtgacgatct ggtaccacca
     3781 gccgcagcgg atggtgcggg cctggggccg gagcgtcgcg gccgcccgcc gctacggcgc
     3841 gcgcgccggc ctccgctacc ggagcctcgt ctggccgccg ggcacggcgc cccgctggca
     3901 gaacacgcgc ttctcgcggc agcggtcctt cgtcgtcgag ctcgcgccgg ggccgctctc
     3961 ggagggacgg gcgctccggc acgcctgggc cgcccgctcg ttcgtcagcc ggtcgtgaag
     4021 acgtagtcct gcggcgcgac
//
LOCUS       DASTFC010000190         6188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_234327,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000190 DASTFC010000000
VERSION     DASTFC010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234327"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFO88_05715"
     CDS             <1..210
                     /locus_tag="VFO88_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545054.1"
                     /translation="LRRLGVLTDEQAEQIKAEAADVMREGIKAAEAEPAADIGLVFEH
                     AYADPPPQLAEDLAELRRILGGSVG"
     gene            203..1177
                     /locus_tag="VFO88_05720"
     CDS             203..1177
                     /locus_tag="VFO88_05720"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9545055.1"
                     /translation="MAEKLLVEAVNDCFHVELERDENVMVMGEDVGRAGGVFRATAGL
                     RDRFGPDRCVDTPLAEAGILGTAVGLCMAGWRPVCEMQYDAFSYPALDQLITHVGRYR
                     WRTGGAMEFPVTIRMPYGGGVRAPELHDDSPETYYVHTPGIKVAIPSTPADAKGLLAA
                     AIRDPDPVVILEPKLHYRTLRGEVPEGEHVTPLGKARLAREGTDLTLVAYGSMVPLSE
                     AAADALAGEASVEVLDLRSLKPLDEEALLASAAKTGRVVIVQEAPRVAGFGAEIAAIL
                     AEKAILDLHGPVLRVTGYDVPYPYWQIEDAYMPSVERVVDAARKLLAF"
     gene            1182..2318
                     /locus_tag="VFO88_05725"
     CDS             1182..2318
                     /locus_tag="VFO88_05725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9545056.1"
                     /translation="MAYEFKLPDLGEGLTEGEIASWLVSEGDEIAEDQPLVEIQTDKT
                     TVEIPSPAAGTVTRILVEEGATVPVGTVLVVIGGDGAEPAAAGDAQPRAEEAPQKEAA
                     DAATTSPRTAPTGGRVRATPLVRRVADELGVDLESVQGTGPQGRITEDDVRAAAAGGA
                     PAAAEGERREPLRGVRRLIAEHLTVSHREVPAVTVVEECDFTALDGVRGERSYLPYVL
                     QATVEGLRAVPELNARLEGGEIVYLERYDLGVAVQTEQGLVVPVLTNADARGLDELEA
                     EAARLAEAARTGSLRPEELRGSTFTVSSGGKLGGLFATPLVNHPEVGILGVHRIADRP
                     VVRDGEIVVGKIGLLACTFDHRVVDGVRASAFLLEVIRRLEAPG"
     gene            complement(2364..2918)
                     /locus_tag="VFO88_05730"
     CDS             complement(2364..2918)
                     /locus_tag="VFO88_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET9545057.1"
                     /translation="MVRKRRVTAVAAAALIVLGAASYAVAGALNGPATPPETKRFKAE
                     LNGFQEVPSVSTTGFGRFHARLVDDELIEYRFEYAGLEGGNSLFAHIHFAQRNVNGGV
                     MVFICGGGSKPDPCPNVEGTVTGTFVPSDVVGPNAQGIEPGSWDEFLRALRAGHAYVN
                     IHTVRWPGGEIRGQINDRDQRTFE"
     gene            complement(3015..3320)
                     /locus_tag="VFO88_05735"
     CDS             complement(3015..3320)
                     /locus_tag="VFO88_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9545058.1"
                     /translation="MTQEAQPLLLFFTAPTSGPARRMESLLAHLARKERARLKVSRIE
                     VDESPQLAEKLGVEEVPTLVLIKDRRPVARLAGRVSAPQIERMLEPYLDPHQTPAAA"
     gene            complement(3369..4211)
                     /locus_tag="VFO88_05740"
     CDS             complement(3369..4211)
                     /locus_tag="VFO88_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545059.1"
                     /translation="MGLGTILALVLVVFLLLPVLGRRALETRRHRALHALEQARGSRV
                     IALIHRQETIGFLGVPLFRFIDIEDSEAVLRAIRTTPDERPLDILLHTPGGLVLASEQ
                     IAYALRDHPGKVTVLVPHYAMSGGTLIALAADEIRMDAAAVLGPVDPQLGDLPAASIV
                     RAAAKKPVERLDDRTIIMADVAEKAVRHMKDVVRSLVTPRMEAEKADSLAEELAGGHY
                     THDDPITLTEARALGLPVEKGIPEEAYALMELFRQAGGRRPSVQYIPLPAPIERGPTR
                     PGRP"
     gene            4249..4632
                     /locus_tag="VFO88_05745"
     CDS             4249..4632
                     /locus_tag="VFO88_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545060.1"
                     /translation="MDGLLDWYLLGLALGLGVAAGIPGLPREEGRGFAVGMVTAVAVA
                     SGVIAALAVVWAVFATLIGVAVGVFSYRKLARPAVPAASLAVAGLSLVPVLGYVVAAF
                     SPVLGERLRRRAAGRYAGLRVLAKD"
     gene            4644..6089
                     /locus_tag="VFO88_05750"
     CDS             4644..6089
                     /locus_tag="VFO88_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HET9545061.1"
                     /translation="MPGAGRKLVLVVIDGLTPELLERGIESGRVPALARLVEEGTYAR
                     GVTTFPSVTPVCLSTLATGAGPDVHHIPHLAWYHRGEERIVDYGSSFPAMRAVGARQA
                     FRDAVFNMSHAHLSARATTVFEAVEDAGLVPASINFTCYRGRTRHAIKLPGLAARNRW
                     YEAVYGPRRFFFFNLFESDETGAPLAVRSRVDGSNDAYAGAIGRWLVTRDGFDFLVFY
                     LPDFDYASHLAGPSSPVDALVRADAAVGQLMEAAGGPDELLARYAVVLCSDHGQTHVD
                     EVARLQDAYADLALLAPRRGDPGAADVAVTASNRSGMVYRLGRCPLDVRDLAERLDEV
                     DAADVVAFREDGEAVVRREREELRFAPGPEGGWRISGDAAVLDEGRYPNGLERVWRAL
                     ANPNAGEVLVSARAGVEFLDFGGRHHAGGGSHGSLVAGDSTVPVVAAGLDAPAVGGRP
                     SLTELAPLALRYLGVPPPASMGAAALEPAGV"
BASE COUNT          758 a         2291 c         2295 g          844 t
ORIGIN      
        1 ctgcgccggc tcggcgtcct gaccgacgag caggcggagc agatcaaggc cgaggcggcg
       61 gacgtgatgc gcgagggcat caaggcggcc gaggcggagc ccgccgccga catcggcctc
      121 gtcttcgagc acgcgtacgc cgacccgccg ccgcagctcg ccgaggacct cgccgagctc
      181 cgtcgcatcc tcggaggctc cgttggctga gaagctcctg gtcgaggcgg taaacgactg
      241 cttccacgtc gagctcgagc gcgacgagaa cgtcatggtc atgggcgagg acgtcggccg
      301 cgcgggcggc gtcttccgcg cgaccgccgg cctccgcgac cgcttcgggc ccgaccgctg
      361 tgtcgacacg ccgctcgcgg aggcggggat cctcggcacc gcggtcggcc tctgcatggc
      421 cggctggcgc cccgtgtgcg agatgcagta cgacgcgttc tcgtaccccg cgctcgacca
      481 gctcatcacg cacgtcggcc gctaccgctg gcgcaccggc ggcgcgatgg agttccccgt
      541 cacgatccgc atgccgtacg gcggcggcgt gcgcgcgccc gagctgcacg acgactcgcc
      601 ggagacgtac tacgtccaca cgccggggat caaggtggcg atcccctcga cgccggccga
      661 cgcgaagggg cttctcgccg ccgcgatccg cgacccggac cccgtcgtca tcctcgagcc
      721 gaagctccac taccggacgc tgcgcggcga ggtgcccgag ggcgagcacg tgacgccgct
      781 cggcaaggcg cgcctcgcgc gcgagggcac ggacctgacg ctcgtcgcgt acggctccat
      841 ggtgccgctt tccgaggcgg cggccgacgc gctcgccggc gaggcgtccg tcgaggtgct
      901 cgacctccgc tcactgaagc cgctcgacga ggaggcgctc ctcgcgtcgg cggcgaagac
      961 cggccgcgtc gtgatcgtcc aggaggcgcc gcgcgtcgcc ggcttcggcg cggagatcgc
     1021 ggcgatcctc gcggagaagg cgatcctcga cctgcacggt cccgtcctcc gcgtgaccgg
     1081 ctacgacgtg ccgtacccgt actggcagat cgaggacgcc tacatgccgt ccgtcgagcg
     1141 cgtcgtcgac gcggcccgga agctcctggc cttctgaccc gatggcgtac gagttcaagc
     1201 tgcccgacct gggcgagggg ctgaccgagg gcgagatcgc gagctggctc gtctccgagg
     1261 gcgacgagat cgccgaggac cagccgctcg tcgagatcca gacggacaag acgacggtcg
     1321 agatcccctc cccggcggct gggacggtga cgaggatcct cgtcgaggag ggcgcgaccg
     1381 tgcctgtcgg gaccgtgctc gtcgtgatcg gcggcgacgg cgccgagccg gcggccgcgg
     1441 gcgacgcgca gccgcgcgcc gaggaggcgc cgcagaagga ggcggcggac gcggcgacga
     1501 cgagccctcg caccgcgccc acgggcggcc gcgtccgcgc gacgccgctc gtccgccggg
     1561 tcgccgacga gctcggcgtc gatctcgagt cggtgcaggg caccggcccg cagggacgga
     1621 tcaccgagga cgacgtccgc gccgccgcgg ccggtggcgc gcccgcggcc gcggagggcg
     1681 agcgccgtga gccgctccgg ggcgtccggc gcctgatcgc cgagcacctg accgtctcgc
     1741 accgcgaggt tcccgccgtc accgtcgtcg aggagtgcga cttcacggcg ctcgacggcg
     1801 tgcgcggcga gcggagctac ctcccgtacg tccttcaggc gacggtggag ggcctgcgcg
     1861 ccgtgcccga gctgaacgcg cggctcgagg gcggcgagat cgtctacctc gagcgctacg
     1921 acctgggggt cgccgtccag acggagcagg gcctcgtcgt cccggtgctc acgaacgcgg
     1981 acgcccgcgg cctcgacgag ctcgaggccg aggccgcccg cctcgccgag gccgcacgga
     2041 cgggcagcct caggccggag gagctgcgcg gctcgacgtt taccgtctcc agcggcggga
     2101 agctcggcgg gctcttcgcc acgccgctcg tgaaccaccc cgaggtcggg atcctcggcg
     2161 tccaccggat cgccgaccgg cccgtcgtcc gcgacgggga gatcgtcgtc ggcaagatcg
     2221 gcctgctggc ctgcacgttc gaccaccgcg tggtcgacgg cgtccgcgcg agcgcgttcc
     2281 tgctcgaggt gatccgccgg ctcgaagcgc ccggatagcc gacggcggcc gcaacgccgc
     2341 cggctccccg tgcgcgttcc gcgttactcg aacgtccgct ggtcgcggtc gttgatctgg
     2401 ccgcggatct cgccgcccgg ccagcgaacc gtatggatgt tcacgtacgc gtggccggcc
     2461 cgcagcgccc gcaggaactc gtcccacgag cccggctcga tcccctgggc gttcggcccg
     2521 acgacatccg acgggacgaa ggtcccggtc acggtgccct cgacgttcgg gcacgggtcg
     2581 ggcttgctcc cgccgccgca gatgaacacc atgaccccgc cgttcacgtt ccgctgcgcg
     2641 aagtggatgt gcgcgaagag cgagttcccg ccctcgaggc ccgcgtactc gaaccggtac
     2701 tcgatcagct cgtcgtcgac gagcctcgca tggaaccgcc cgaatccggt cgtcgaaacc
     2761 gacggcacct cctggaatcc gttcagctcg gccttgaacc tcttcgtctc gggcggtgtg
     2821 gcggggccgt tcaacgcgcc cgcgaccgcg tacgaggcgg cgccgaggac gatgagcgcg
     2881 gcggccgcga ccgctgtcac acgtcgcttc cggaccatgc tttccctcct tctccagcca
     2941 tctgcggcgt cggcagggtt cgcccgccgg gcgccctctc cgggaggctg tacggaggga
     3001 accctcgcgc ggttttacgc ggcggccggc gtctggtgcg ggtcgaggta cggctcgagc
     3061 atccgctcga tctgcggcgc gctcacgcgg cccgcgagcc gcgccaccgg acgacggtcc
     3121 ttgatgagca cgagcgtcgg gacttcctcg acgccgagct tctccgcgag ctgggggctc
     3181 tcgtcgacct cgatccggga caccttgagc cgggcgcgtt ccttccgcgc gagatgcgcg
     3241 aggagactct ccatccgccg tgcgggcccg gacgtggggg cggtgaagaa gaggaggagg
     3301 ggttgcgctt cctgcgtcac gacctacggg ttctccccgt ctcgacccgg cgcgaaacgg
     3361 ctcgcccgct aggggcgccc ggggcgcgtc gggccgcgct cgatcggcgc cgggagcggg
     3421 atgtactgga cgctcggccg ccgcccgccc gcctggcgga agagctccat gagcgcgtac
     3481 gcctcctccg ggatcccctt ctcgacgggc aggccgagcg cacgcgcctc cgtgagcgtg
     3541 atcgggtcgt cgtgcgtgta gtggccgccc gcgagctcct cggcgagcga gtccgccttc
     3601 tccgcctcca tccgcggcgt gacgagcgag cggacgacgt ccttcatgtg ccgcacggcc
     3661 ttctcggcga cgtcggccat gatgatcgtc cggtcgtcga gacgctcgac cggcttcttc
     3721 gccgccgcgc ggacgatcga ggcggccggg aggtcgccga gctgcgggtc gacggggccg
     3781 agcacggcgg cggcgtccat gcggatctcg tcggccgcca gggcgatgag cgtcccgccg
     3841 gacatcgcgt agtgcgggac gagcacggtc accttccccg gatggtcgcg cagggcgtac
     3901 gcgatctgct cggacgcgag cacgaggccg cccggggtgt ggaggaggat gtcgagcggg
     3961 cgctcgtccg gcgtcgtccg gatcgcgcgg agcaccgcct cggagtcctc gatgtcgatg
     4021 aagcggaaga gcgggacgcc gaggaagccg atcgtctcct gccggtggat gagcgcgatc
     4081 acgcgcgagc cgcgcgcctg ctcgagggcg tggagggcgc ggtgccgccg cgtctcgagc
     4141 gcccgccggc cgagcaccgg gagcaggagg aacacgacga gcacgagcgc gaggatcgtc
     4201 ccgaggccca tcgggcgagc atagacgcgg cgtcactaca ctggccgcgt ggacgggctc
     4261 ctcgactggt acctgctcgg gctcgcgctc ggcctcggcg tcgccgcggg gattcccggg
     4321 ctgccgcgcg aggaggggcg gggcttcgcg gtcgggatgg tgaccgccgt cgcggtggcg
     4381 agcggcgtga tcgcggcgct cgcggtcgtc tgggcggtgt tcgcgaccct gatcggcgtc
     4441 gcggtcggcg tcttctccta ccggaagctc gcacgccccg ccgtgcccgc cgcgtcgctc
     4501 gccgtcgcgg ggctctcgct cgtcccggtg ctcggctacg tcgtcgcggc gttctcgccc
     4561 gtcctcggcg agcgcctgcg ccgccgggcc gccggccgct acgcggggct tcgcgtcctc
     4621 gccaaggact gaggcggccc cgcgtgccgg gcgccggtcg caagctcgtc ctcgtcgtca
     4681 tcgacgggct gacgcccgag ctgctcgagc gcgggatcga gtccggccgc gtgccggcgc
     4741 tcgcgcggct cgtggaggag gggacgtacg cgcgcggcgt gacgacgttt ccgtccgtca
     4801 cgcccgtctg cctctcgacg ctcgccaccg gcgcggggcc ggacgtccac cacatccccc
     4861 acctcgcctg gtaccaccgg ggggaggagc ggatcgtcga ctacggctcc tcgttcccgg
     4921 ccatgcgcgc ggtcggcgcg cggcaggcgt tccgcgacgc ggtcttcaac atgagccacg
     4981 cgcacctgag cgcccgggcg acgacggtct tcgaggcggt cgaggacgcg ggcctcgtcc
     5041 cggcctcgat caacttcacg tgctaccggg ggcgcacccg gcacgcgatc aagctgcccg
     5101 gtctcgccgc ccggaaccgc tggtacgagg cggtgtacgg gccgcggcgg ttcttcttct
     5161 tcaacctctt cgagtcggac gagaccgggg cgccgctcgc ggtccgctcg cgcgtggacg
     5221 gctcgaacga cgcctacgcg ggggcgatcg ggcgctggct cgtcacgcgc gacgggttcg
     5281 acttcctcgt cttctacctg ccggacttcg actacgcctc gcacctcgcc gggccgtcct
     5341 cacccgtcga cgcgctcgtg cgcgccgacg cagccgtggg gcagctcatg gaggccgccg
     5401 gcgggccgga cgagctgctc gcgcgctacg cggtcgtgct ctgctccgac cacgggcaga
     5461 cgcacgtcga cgaggtggcg cgcctccagg acgcctacgc ggacctggcc ctcctcgcgc
     5521 cgcggcgcgg cgacccgggc gcggcggacg tggcggtcac ggcgtcgaac aggagcggga
     5581 tggtctaccg cctcgggcgc tgcccgctcg acgtgcgcga cctcgccgaa cgcctcgacg
     5641 aggtcgacgc ggccgacgtg gtcgcgttcc gggaggacgg ggaggcggtc gtcaggcggg
     5701 agcgcgagga gctgcgcttc gcgcccggcc cggaaggcgg ctggcggatc tccggcgacg
     5761 cggccgtgct cgacgagggg cgctacccga acggcctcga gcgcgtctgg cgcgcgctcg
     5821 cgaacccgaa cgccggcgag gtcctcgtgt cagcgcgcgc gggggtcgag ttcctcgact
     5881 tcggcggccg tcaccacgcc ggcggcggca gccacggctc gctcgtcgcc ggggactcga
     5941 cggtgccggt cgtcgccgcc ggcctcgacg ccccagccgt cggcggccgg ccgtcgctca
     6001 ccgagctcgc gccgcttgct ctgcggtacc tcggcgtgcc gccccccgcc tccatgggcg
     6061 cggccgccct cgagcccgcc ggcgtctgag cgccgtggac tacgagcggc tccgcgagcg
     6121 gatggtcgag cgccagctcg cccggcgcgg gatccgggac gagcgcgtcc tcgaggcgat
     6181 gcgccgcg
//
LOCUS       DASTFC010000191         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_910159,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000191 DASTFC010000000
VERSION     DASTFC010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910159"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /gene="era"
                     /locus_tag="VFO88_05755"
     CDS             <1..864
                     /gene="era"
                     /locus_tag="VFO88_05755"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9545062.1"
                     /translation="VRSGFVAVAGRPNVGKSTLVNALCGGKVAIVSDKPQTTRRRISG
                     IANSPGHQLVLQDLPGFQRPRDPLTERMQRTVDESLGDDVDGILFVLAADERLGGGDR
                     FIAGRVFTGERPVVIALNKVDRLKPDRIAARIAEAAALGDFHALHPVSALTGDGVDAL
                     RADLVELLPEGPAYFPEGDVTDLSLEARVAELVREAALALTREEVPHAVMSQTEELEE
                     RRVAVTLFVETDSQKGILVGKGGRMIREIGSRARPEIEALLGRGVFLELTVKVRPKWR
                     RDEAMLTRMGI"
     gene            874..2379
                     /locus_tag="VFO88_05760"
     CDS             874..2379
                     /locus_tag="VFO88_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545063.1"
                     /translation="MGTVTADRFEERLRSYLAESSEEARAVRVGEKETSDHAAIVARY
                     ADLFTRDQHAALAEAADAAPEAERERLVRLREAAAGGVLSLELAEAYDALQNKVLAAR
                     LEFKGEEMPLRTAQAQVALLDDYADREELGRISGDLSATFNDERLDLMRRGEELEAEL
                     TGEPDPIRRSADLKGIDLHELSTALDRTAERQAPAWGPLRARWADRLLGDERDDEPTS
                     AHVAYLRRMSPLADTYTKDKAVPVCLATLARLGFDLANESRIRLDLDDRPQKSPRACV
                     IAADPPEIVHLITRAQGGLHDYQALLHEAGHALHYAGCDPALPYTFRRLSRDHALTEL
                     YSFLVESISREPGWHAAHFDLEDDEAAERAEGTRFLEALLFRRYAAKLSYELELFADF
                     EHATRFSEVYAERLRAAVGFRYRPDNYLADLDDGFYSADYLRAWIRSAQLRRHLRTTV
                     GEDWWERRETGELLRGLFREGTRPSSEEVAQRIGFDPLDTEPLLDELAAVA"
     gene            2485..2634
                     /locus_tag="VFO88_05765"
     CDS             2485..2634
                     /locus_tag="VFO88_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545064.1"
                     /translation="MTTYALENRTARRATHDETPLRFRLYSAVYAVLAVAGLAFLALV
                     GVAAI"
     gene            complement(2631..3308)
                     /locus_tag="VFO88_05770"
     CDS             complement(2631..3308)
                     /locus_tag="VFO88_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545065.1"
                     /translation="MPLPLAAGSLQGLSLLRAGLNRVIGLPDLLASLGGLRPSKRLRL
                     QVVLLRDEEGEPVAPLAEVEAALAETARVLELAARTRLVPTGDSLVVTADGPAPAAAL
                     DAPCTSQGLWRTDLGPAGGFFRGLRVRGGLRFIGRGAPLTVFVVRDVVGKCGCSLGPL
                     GDYVTVDAEGLGGRTRRILAHELGHSCGLRHKPDERNLMRPKGPGEELTAWQTAVFRS
                     SRHVTYL"
     gene            3370..4308
                     /gene="deoC"
                     /locus_tag="VFO88_05775"
     CDS             3370..4308
                     /gene="deoC"
                     /locus_tag="VFO88_05775"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET9545066.1"
                     /translation="MAKAPALPGGFELPLEPRLPRVGSVDQVGVEERSASLARRSIKK
                     EAKLFALELAVRMTDLTTLEGSDTRGKVTALASKAAHPDPSDPTIPPVAALCIYPDLI
                     PVARERLEGTPVKIAAVATAFPSGRAPLDLKVEEARRAAEAGADEIDMVIDRGAMLSG
                     AYAKVYEEVRQVKEAVGDAHLKVILETGELGTYDNVRRASLLAIAGGADFVKTSTGKI
                     SPAATLPVTLVMLEAVRDVYEETGKKIGVKPAGGIREAKQAVQHLVAVNETLGPEWLT
                     PDLFRFGASSLLNDLLLQIRKQKTGQYQSPDYFTLD"
     gene            4482..>4886
                     /locus_tag="VFO88_05780"
     CDS             4482..>4886
                     /locus_tag="VFO88_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HET9545067.1"
                     /translation="MTQRKTLKRRVRARMEKTGERYTAARAHVVGEPAPEPVQEPDLS
                     GLASEDALVAATGRRWKEWFAVLDAWGANERKHGEIARRLRSEHGVPGWWSQTVTVGY
                     ERARGLRVKHERPDGFSVSVSRTVAAPAERLYA"
BASE COUNT          697 a         1811 c         1766 g          612 t
ORIGIN      
        1 gtgaggtcgg ggttcgtcgc cgtcgccggc cgcccgaacg tcggcaagtc gacgctcgtg
       61 aacgcgctct gcggcggcaa ggtggcgatc gtctcggaca agccgcagac gacccgccgc
      121 cgcatctcgg gcatcgcgaa cagccccggc caccagctcg tgctccagga cctgcccggc
      181 ttccagcgcc cgcgcgaccc gctcaccgag cggatgcaga ggacggtgga cgagtcgctc
      241 ggagacgacg tcgacgggat cctcttcgtg ctcgccgcgg acgagcggct cggcggcggc
      301 gaccgcttca tcgccgggcg cgtcttcacg ggcgagaggc cggtcgtgat cgcgctgaac
      361 aaggtcgacc gcctgaagcc cgaccggatc gcggcgcgca tcgccgaggc ggccgcgctc
      421 ggcgacttcc acgcgcttca cccggtgagt gcgctcaccg gggacggcgt cgacgccctg
      481 cgcgccgacc tggtcgagct tctccccgag ggccccgcct acttccccga gggcgacgtc
      541 accgacctgt cgctcgaggc ccgcgtcgcg gagctcgtcc gcgaggcggc gctcgccctc
      601 acccgcgagg aggtcccgca cgcggtcatg tcgcagaccg aggagctcga ggagcggcgc
      661 gtcgcggtca cgctcttcgt ggagacggac tcccagaagg ggattctcgt cggcaagggc
      721 ggccgtatga tccgggagat cggctcgcgg gcccggccgg agatcgaggc gctgctcggt
      781 cggggggtgt tcctcgagct gaccgtgaag gtgcggccga agtggcgtcg cgacgaggcg
      841 atgctcacgc gaatggggat ctgaggaggc tcgatgggga cggtgacggc ggaccggttc
      901 gaagagcggc tgcgcagcta cctcgcggag tcctccgagg aggcccgcgc ggtgcgcgtg
      961 ggcgagaagg agacgagcga ccacgccgcg atcgtcgcgc gctacgccga tctcttcacc
     1021 cgcgaccagc acgcggcgct ggccgaggcg gccgacgcgg cgcccgaggc cgagcgcgag
     1081 cggctcgtcc gcctccgcga ggcggccgcc ggcggggtcc tctcgctcga gctcgccgag
     1141 gcgtacgacg cgctccagaa caaggtgctc gccgcccggc tcgagttcaa gggcgaggag
     1201 atgccgctgc gcacggcgca ggcgcaggtc gcgctcctcg acgactacgc ggaccgcgag
     1261 gagctcggcc ggatctccgg cgacctctcc gcgacgttca acgacgagcg cctcgacctc
     1321 atgcgccgcg gcgaggagct cgaggccgag ctgaccggcg agccggaccc gatccggcgc
     1381 agcgccgacc tcaaggggat cgacctccac gagctctcca cggcgctcga ccggacggcg
     1441 gagcgccagg cgcccgcgtg gggcccgctg cgcgcgcgct gggccgaccg gctcctcggc
     1501 gacgagcgcg acgacgagcc gacgtccgcg cacgtcgcct acctgcgccg gatgtcgccg
     1561 ctcgcggaca cgtacacgaa ggacaaggcg gtgcccgtct gcctcgcgac gctcgcgcgg
     1621 ctcgggttcg acctcgcgaa cgagtcgcgc atcaggctcg acctcgacga ccggccccag
     1681 aagagcccgc gcgcgtgcgt gatcgccgcc gacccgcccg agatcgtcca tctcatcacc
     1741 cgcgcgcagg gcggcctcca cgactaccag gcgctcctgc acgaggccgg ccacgcgctc
     1801 cactacgccg gctgcgaccc ggcgctcccg tacacgttcc ggcgcctctc gcgcgaccac
     1861 gcgctgaccg agctctactc gttcctcgtc gagtcgatca gccgcgagcc cggctggcat
     1921 gcggcccatt tcgacctcga ggacgacgag gcggccgagc gtgcggaggg gacgcggttc
     1981 ctcgaggcgc tcctcttccg ccgctacgcc gcgaagctct cgtacgagct cgagctcttc
     2041 gccgacttcg agcacgcgac gcgcttctcc gaggtctacg cggagaggct ccgagcggcc
     2101 gtcggcttcc gctaccggcc ggacaactac ctcgccgacc tcgacgacgg gttctactcg
     2161 gccgactacc tccgggcgtg gatccgctcc gcgcagctgc gccgccacct gcgcacgacc
     2221 gtcggcgagg actggtggga gcggcgggag accggcgagc tcctgcgcgg gctcttccgc
     2281 gagggcacgc ggccgtcgag cgaggaggtg gcgcagcgga tcggcttcga cccgctcgac
     2341 accgagccgc tgctcgacga gctcgcggcc gttgcctgag cgggcgttcg ggtttccgca
     2401 ccgtcgtcca cgaggaggca ccattccgcc cgccgcgcgg tcccgcgagg cttcggtcgt
     2461 ccccagaaac gaaaggaacc caggatgacg acctacgccc tcgagaaccg caccgccagg
     2521 cgcgcaaccc acgacgagac cccgcttcgc ttccggctct actcggccgt ctacgccgtc
     2581 ctcgccgtcg ccgggctcgc cttcctcgcc ctggtcggcg tggcggcgat ctagagatac
     2641 gtcacgtggc gtgaggagcg gaagaccgcc gtctgccagg cggtcagctc ctcacccggg
     2701 cccttcgggc gcatgaggtt gcgctcgtcg ggcttgtggc gcaggccgca ggagtgcccg
     2761 agctcgtgcg cgaggatgcg ccgcgtcctg ccgccgagcc cctccgcgtc gaccgtgacg
     2821 tagtcgccga gcggcccgag cgagcagccg cacttcccga cgacgtcgcg cacgacgaag
     2881 accgtgagcg gcgcgccgcg cccgatgaac cggaggcctc cgcggacgcg cagcccgcgg
     2941 aagaacccgc cggccggccc gaggtcggtg cgccagagcc cctgggaggt gcacggcgcg
     3001 tcgagcgcgg ccgccggcgc gggcccgtcc gcggtcacga cgagcgagtc gcccgtcgga
     3061 acgagccgcg ttcgggccgc tagctcgagc acgcgcgcgg tctcggcgag cgccgcctcc
     3121 acctccgcga gcggggcgac gggctcgccc tcttcgtccc gcagcaggac gacctggagc
     3181 cgcagccgct tcgacgggcg cagcccgccg agcgacgcga gcagatcggg cagcccgatc
     3241 acgcggttga gacccgcgcg gaggagcgag aggccctgga gcgagcccgc ggcgaggggc
     3301 agcggcatgc ggctctccta cacgagcccg gcgtgcggcg tcaagacgcg cggctctaca
     3361 ctgagcggaa tggcgaaagc gcctgcactt cccggcggct tcgagctccc gctcgagccg
     3421 cgcctcccgc gcgtcggctc ggtcgaccag gtcggggtcg aggagcgctc cgcctcgctc
     3481 gcgcggcgct cgatcaagaa ggaggcgaag ctcttcgcgc tcgagctcgc cgtcaggatg
     3541 accgacctca cgacactcga gggctcggac acgcgcggga aggtgaccgc gctcgcctcg
     3601 aaggccgcgc accccgatcc gtccgatccg acgatcccgc ccgtcgcggc gctctgcatc
     3661 taccccgacc tgatcccggt cgcgcgcgag cggctcgagg ggactcccgt gaagatcgcg
     3721 gccgtcgcga ccgcgttccc gtccggccgc gcgccgctcg acctcaaggt cgaggaggcg
     3781 cgccgtgccg ccgaggccgg cgccgacgag atcgacatgg tcatcgaccg cggcgcgatg
     3841 ctctcgggcg cgtacgcgaa ggtctacgag gaggtccggc aggtgaagga ggcggtcggc
     3901 gacgcgcacc tgaaggtcat tctcgagacc ggcgagctcg ggacgtacga caacgtccgc
     3961 cgcgcgagcc tcctcgcgat cgcgggcggc gccgacttcg tcaagacctc gacggggaag
     4021 atcagcccgg ccgcgacgct cccggtcacg ctcgtcatgc tcgaggccgt tcgcgacgtc
     4081 tacgaggaga ccggcaagaa gatcggcgtg aagccggcgg gcgggatccg ggaggcgaag
     4141 caggccgtcc agcacctcgt ggccgtgaac gagacgctcg gccccgagtg gctgacgccc
     4201 gacctgttcc gcttcggcgc atcctcgctc ctgaacgacc tcctgctcca gatccgcaag
     4261 cagaagaccg ggcagtacca gagccccgat tacttcacgc tcgactgaga gtcttctaga
     4321 gtgatggacg gcgccgtccc gcgcccaacc tccgctccgg cgctcacgcc gccggcccgg
     4381 gaggcaaagg gggcttctgc tctcgggacg cacgatcact tcgcctgggc cgcggcccgg
     4441 gagcgtgagt cccgcgtcgc cggccggtga ggtggctgta gatgacacag cggaagaccc
     4501 tgaagcggcg ggttcgcgcc cgcatggaga agacgggcga gcgctacacc gcggcgcgcg
     4561 cacacgtcgt cggcgagccc gcgccggagc ccgttcagga gccggacctg agcggcctcg
     4621 cgtccgagga cgccctcgtc gcggcgaccg ggcgccggtg gaaggagtgg ttcgccgtcc
     4681 tcgacgcctg gggcgcgaac gagcggaagc acggcgagat cgcccggcgc ctgcggtcgg
     4741 agcacggcgt tcccggctgg tggtcgcaga ccgtgaccgt cgggtacgag cgggcgcgcg
     4801 gcctccgcgt gaagcacgag cggccggacg gcttctccgt ctcggtgtcg cggacggtcg
     4861 cggcgccggc cgagcggctg tacgcg
//
LOCUS       DASTFC010000192         5761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_201479,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000192 DASTFC010000000
VERSION     DASTFC010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201479"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFO88_05785"
     CDS             <1..335
                     /locus_tag="VFO88_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545068.1"
                     /translation="ADDFAYLVRTPARAAAVAFGKAGKTIDDVQRVEINEAFASVALN
                     STRLLGADEEIVNVNGGAVALGHPIGASGGRIVGTMVHELRRNGGGLGLAAICSGGGQ
                     GDALLLEV"
     gene            354..854
                     /locus_tag="VFO88_05790"
     CDS             354..854
                     /locus_tag="VFO88_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545069.1"
                     /translation="MRRLAFVAALALAATAAGARPDCAYACTCAPLVAEQALAAADAA
                     VVGTVLERRVSGGEATLVVSVERRLKGPLGERVEVATPADDATCGIGAQPGTRLGLFL
                     RREGGGWRGDLCGQARPEALASLPAAAGGEEVADSFAEATAYVLAVLVAGAIGVFLLR
                     RRFRPD"
     gene            866..1696
                     /locus_tag="VFO88_05795"
     CDS             866..1696
                     /locus_tag="VFO88_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9545070.1"
                     /translation="MEFGHVLVVGAGQMGGGIAQVVAASGRRVSLHDPLPGATDRALD
                     AIGRSLEKLAEKGGPPPDEVLPRIGAVEELVPAELMIEAVVEDAGVKEDVFRRADAAL
                     PAEAILASNTSSIPIASLAAATQRPDRVIGMHFFNPVPVMALVEVVRTPQTSDETAAA
                     VVELAHELGKTPAEANDFPGFVSNRILMPYINEAVWALHDGVAEPEAIDTIAKLGFNH
                     PMGPLALADLIGLDTCVAILEVLEAGLRDARYAPCPLLREHVDAGRLGRKSGQGFYSY
                     "
     gene            1787..2851
                     /locus_tag="VFO88_05800"
     CDS             1787..2851
                     /locus_tag="VFO88_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9545071.1"
                     /translation="MRLLLIDDDPGLRALVRTTFEAVDVDVEEAEDGIVARTLVTDRR
                     PDAIVLDLTMPGVTGFDLCRELKADGDTSTIPIVLLTGDTASEEDARDAGADAFMRKP
                     FSPLELLAVVERLAGGLYGVPFRASGKQKAEEQLLLYARDLRHLLEIERGQRVLLQKA
                     YQETVSALASALETKDTGTRAHSQRVQRYAIELTRAVSPALGEDQSAQYGFLLHDVGK
                     IGIPDDILRKPRPLSEAEERLMRTHTVLGEQMLSGVAFLQGTGLAVVRHHHERWDGDG
                     YPDRLRRQEIPLGARIFAVADSLDAITSDRPYREARSWEFARTEIEDEAGRQFDPDVV
                     AAFVDVEPTLREIAREFVAA"
     gene            complement(2848..4047)
                     /locus_tag="VFO88_05805"
     CDS             complement(2848..4047)
                     /locus_tag="VFO88_05805"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET9545072.1"
                     /translation="MRIPVASLARVRPARDDDGVTTFELFFDLVFVFAVTQVTGFMAH
                     EGDGVGVVRGLLLLALLWWAWEAYAWLGNQARADEGLTRAGMIVAMAAIFVVALTVPE
                     AWEDLPGGVDGPVVLVTAYVVARIVHLAVYNAAALGDEGLRRQIAITFVPMLSGAALL
                     FLGALVGGSAQTALFAAGLAVDWAGTYVTSKEGNWRIHSPRHWSERHGLFVILAIGES
                     IVAIGVGAAQLPISIPLLLGAVLGLLVSVCLWWLYFDLVSLAAEHVLVDATPAERVKI
                     AVDAYTYLHFPLVAGIVLAALGVEEVLAHAEDTERLGTFAAAALAGGLALYLGGHLAF
                     KRRMHGQLNALRLGAALVLVASIPLCAVLPPLGALAVAFGVLAALVAAESVVHAEKRR
                     GLRGLEG"
     gene            4090..5250
                     /locus_tag="VFO88_05810"
     CDS             4090..5250
                     /locus_tag="VFO88_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9545073.1"
                     /translation="MDFELTSEQREIQAVAREFADAEIAPHAAEWDREHRFPHELVPK
                     LAELGFMGVCIPEELGGVGADFLSYMLVLEEISRADAGIGVTVAVHTSAATLPIARLG
                     TDEQREALVPPMARGEKIGAFALTESGAGSDARSLRTRAEADGDGWRLTGTKQWITSG
                     SFGGTVLVFARGDEGISCFVVDGEADGLSVSKEHSKMGLHSSSTVDLALDGVLVPRDR
                     LLGEEGKGFRIAMSTLDGGRIGIAAQALGIAQAAYDAARAYALERRQFGRRIGEFGAI
                     QEKVADMATELDAARLLVYRAAWLRDRGEPHGEAGAKAKLYASSVARRWTGEAIQILG
                     GYGFTTDFPVERYYRDAKITEIYEGTSEIQRLVIARSVLGLAERAEPVAAGA"
     gene            5255..>5761
                     /locus_tag="VFO88_05815"
     CDS             5255..>5761
                     /locus_tag="VFO88_05815"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9545074.1"
                     /translation="MTRRRDEPVRMDRIYTRGGDAGETSLGDGARVPKDSPRIEAYGD
                     VDELNAVLGLALARGLPEPAASWLTIVQNELFDLGADLAVPLGDARERLRVTAGQVER
                     LEAWCDEANEGLPMLRSFVLPGGSEAAALLHLGRTVCRRAERRAVTLAEREEVNPQAL
                     AYLNRLSDL"
BASE COUNT          813 a         2077 c         2158 g          713 t
ORIGIN      
        1 tcgccgacga cttcgcgtac ctcgtccgca ctcccgcccg tgctgccgcc gtcgcgttcg
       61 ggaaggcggg gaagacgatc gacgacgtcc agcgggtcga gatcaacgag gcgttcgcgt
      121 ccgtcgcgct caactcgacg cgcctgctcg gagcggacga ggagatcgtg aacgtgaacg
      181 gcggggccgt cgcgctcggg cacccgatcg gcgcctccgg cgggcggatc gtcggcacga
      241 tggtgcacga gctgcgccgg aacggcggcg gcctcggcct cgccgccatc tgctcgggcg
      301 gcggccaggg cgacgcgctg ctgctcgagg tctgagcgcg gcgccggcgc ggcgtgaggc
      361 gcctcgcgtt cgtcgccgcc ctcgccctcg cggcgaccgc cgcgggtgct cgtcccgact
      421 gcgcatacgc ctgcacttgc gcgcctctcg tcgccgagca ggcgctcgcg gccgccgacg
      481 ccgccgtcgt cgggacggtg ctcgagcgcc gggtctccgg cggcgaggcg acgctcgtcg
      541 tgtccgtcga gcggcggctc aaggggccct tgggggagcg ggtcgaggtc gcgacgcccg
      601 ccgacgacgc gacgtgcggc atcggggcgc agcccggcac gcgcctcggc ctcttcctca
      661 ggcgcgaggg cggcggctgg cgcggcgacc tctgcggcca ggcgcggccc gaggcgctcg
      721 cctcgctccc ggccgccgcg ggcggggagg aggtggcgga ctcgttcgcg gaggcgacgg
      781 cctacgtgct cgcggtcctc gtcgccggcg cgatcggcgt cttcctgctg cggaggcgct
      841 tccggcccga ctaggctccc gcgccgtgga gttcgggcac gtgctcgtcg tcggcgcagg
      901 gcagatgggc ggcggcatcg cgcaggtggt ggccgcctcc ggccgccgcg tctcgctcca
      961 cgacccgctc cccggcgcga cggaccgcgc gctcgacgcg atcggacgca gcctcgagaa
     1021 gctggcggag aagggcgggc cgcctccgga cgaggtgctg ccgagaatcg gggccgtcga
     1081 ggagctcgtt ccggcggagc tgatgatcga ggcagtcgtc gaggacgcgg gcgtgaagga
     1141 ggacgtcttc cgccgggcgg acgcggcgct gcccgcggag gcgatcctcg cctcgaacac
     1201 gtcctcgatc ccgatcgcct cgctcgccgc ggccacgcag cggcccgacc gcgtgatcgg
     1261 gatgcacttc ttcaacccgg tgccggtgat ggcgctcgtc gaggtcgtgc gtacgccgca
     1321 gacctcggac gagaccgccg ccgccgtcgt cgagctcgcc cacgagctcg gcaagacccc
     1381 ggcggaggcg aacgacttcc cggggttcgt ctcgaaccgg atcctcatgc cgtacatcaa
     1441 cgaggcggtc tgggcgctcc acgacggggt ggcggagccg gaggcgatcg acacgatcgc
     1501 gaagctcggc ttcaaccacc cgatgggccc cctcgcgctg gccgacctca tcgggctcga
     1561 cacgtgcgtc gcgatcctcg aggtgctcga ggcgggcctc cgcgacgcgc gctacgcgcc
     1621 ctgccccctc ctgcgcgagc acgtcgatgc gggtcgcctc ggccggaagt ccggccaggg
     1681 gttttactcg tattgacgaa ttcgtcaagg aatcatcaac tacagtcgaa aaagaccgca
     1741 gcaggcggaa aatgagatgc gtcctcgaaa ggaggggagg gccgcaatgc gtctcttgct
     1801 gatcgacgac gacccggggc tccgagctct cgttcgcacg acgttcgagg cggtcgacgt
     1861 cgacgtcgag gaggcggagg acggcatcgt cgcgcggacg ctcgtcaccg accggcggcc
     1921 cgacgcgatc gtcctcgacc tgaccatgcc gggcgtgacc gggttcgacc tctgccggga
     1981 gctgaaggcg gacggggaca cgagcacgat cccgatcgtc ctcctcacgg gggacacggc
     2041 ctccgaggag gacgcgcgcg acgccggcgc ggacgcgttc atgcgcaagc ccttcagccc
     2101 gctcgagctg ctcgcggtcg tcgagcgcct cgcgggcggc ctctacggcg tccccttccg
     2161 ggcgagcggc aagcagaagg ccgaggagca gctcctgctc tacgcgcgcg acctgcgcca
     2221 cctgctcgag atcgagcgcg gccagcgcgt gctcctccag aaggcgtacc aggagacggt
     2281 ctccgcgctc gcgagcgccc tcgagacgaa ggacaccggc acgcgcgcac actcccagcg
     2341 cgtccagcgc tacgcgatcg agctcacccg cgccgtctcg cccgcgctcg gcgaggacca
     2401 gagcgcgcag tacgggttcc tgctccacga cgtcggcaag atcgggatcc cggacgacat
     2461 cctccgcaag ccgcgcccgc tctccgaggc ggaggagcgc ctcatgcgga cgcacaccgt
     2521 gctcggcgag cagatgctga gcggcgtcgc cttcctccag ggcaccgggc tcgcggtcgt
     2581 ccggcaccac cacgagcgct gggacggcga cggctacccg gaccggctcc gccggcagga
     2641 gatcccgctc ggcgcgcgca tcttcgcggt cgccgactcg ctcgacgcga tcacgagcga
     2701 ccggccgtac cgggaagcgc ggtcgtggga gttcgcgcgg acggagatcg aggacgaggc
     2761 gggccgccag ttcgacccgg acgtcgtggc cgcgttcgtc gacgtcgagc cgacgctccg
     2821 cgagatcgcc cgcgagttcg tcgccgccta gccctcgagc ccgcgcagcc cgcgccgctt
     2881 ctcggcgtgg acgaccgact cggcggccac gagcgccgcg agcaccccga acgcgaccgc
     2941 gagcgccccg agcggcggga gcacggcgca gagcgggatc gacgccacga gcacgagcgc
     3001 cgcgccgagc cggagcgcgt tcagctgccc gtgcatcctg cgcttgaacg cgaggtgtcc
     3061 tccgaggtag agggcgagcc ctccggcgag cgcggccgcc gcgaacgtcc cgagccgctc
     3121 ggtgtcctcc gcatgggcga gcacctcctc gaccccgagg gccgccagca cgatccccgc
     3181 cacgagcggg aagtggaggt acgtgtaggc gtcgacggcg atcttcacgc gctcggccgg
     3241 cgtcgcgtcg acgagcacgt gctcggccgc gagcgacacg aggtcgaagt agagccacca
     3301 gaggcagacg gacacgagca ggccgagcac cgctccgagg aggagcggga tcgagatcgg
     3361 aagctgcgcc gcgccgacgc cgatcgcgac gatcgactcc ccgatcgcga ggatcacgaa
     3421 gaggccgtga cgctcgctcc agtgcctcgg gctgtggatc cgccagttgc cctccttcga
     3481 cgtgacgtac gtcccggccc agtcgaccgc gagcccggcg gcgaagagcg cggtctgcgc
     3541 gctgccgccg acgagcgcgc cgaggaagag gagcgccgcg ccggagagca tcggcacgaa
     3601 cgtgatcgcg atctgccgcc gcaggccctc gtccccgagc gcggccgcgt tgtagaccgc
     3661 gaggtggacg atccgcgcga cgacgtacgc ggtgacgagc acgaccggcc cgtcgacgcc
     3721 gccggggagg tcctcccacg cctccggaac cgtgagggcg acgacgaaga tcgccgccat
     3781 cgcgacgatc atccccgccc gcgtgagccc ctcgtccgcg cgcgcctggt tcccgagcca
     3841 cgcgtaggcc tcccaggccc accagaggag cgcgaggagc agcagcccgc ggacgacccc
     3901 gacgccgtct ccctcgtgcg ccatgaagcc cgtcacctgc gtcaccgcga agacgaagac
     3961 gaggtcgaag aagagctcga acgtcgtcac cccgtcgtcg tcgcgggcgg ggcggacacg
     4021 ggcgagcgag gcgacgggga tgcgcacggc cggagggtac gcggcgccgc ccgcgcctac
     4081 aatcgtcgcg tggacttcga gctcacgtcg gagcagcgtg agatccaggc ggtcgcgcgc
     4141 gagttcgccg acgccgagat cgcgccgcac gcggcggagt gggaccgcga gcaccgcttc
     4201 ccgcacgagc tcgtcccgaa gctcgcggag ctcggcttca tgggcgtctg catccccgag
     4261 gagctcggcg gcgtcggcgc ggacttcctc tcgtacatgc tcgtgctcga ggagatctcg
     4321 cgcgccgacg cggggatcgg ggtcacggtc gccgtgcaca cgagcgcggc gacgctgccg
     4381 atcgcgaggc tcgggacgga cgagcagcgc gaggccctcg tcccgccgat ggcgcgcggg
     4441 gagaagatcg gggcgttcgc gctcacggag tcgggcgcgg ggtcggacgc gcgctcgctc
     4501 cgcacccggg ccgaggccga cggcgacggc tggcgcctca ccgggacgaa gcagtggatc
     4561 acgagcggct cgttcggcgg gacggtgctc gtcttcgcgc gcggcgacga gggcatctcc
     4621 tgcttcgtcg tggacggcga ggccgacggc ctgagcgtct cgaaggagca ctcgaagatg
     4681 gggcttcact cgtcgtcgac ggtcgacctc gcgctcgacg gcgtgctcgt gccccgcgac
     4741 cgcctgctcg gcgaggaggg gaaggggttc cggatcgcca tgtcgacgct cgacggcggc
     4801 cggatcggga tcgcggcgca ggcgctcgga atcgcgcagg cggcctacga cgcggcgcgc
     4861 gcgtacgcgc tcgagcggcg gcagttcggc aggcggatcg gcgagttcgg cgcgatccag
     4921 gagaaggtcg cggacatggc caccgagctc gacgcggcgc gcctgctcgt ctaccgcgca
     4981 gcgtggcttc gcgaccgcgg cgagccgcac ggcgaggcgg gggcgaaggc gaagctctac
     5041 gcgtcgtccg tcgcgcgccg gtggaccggc gaggcgatcc agatcctcgg cggctacggc
     5101 ttcacgaccg acttcccggt cgagcggtac taccgcgacg cgaagatcac ggagatctac
     5161 gagggcacga gcgagatcca gcggctcgtg atcgcgcgct cggtgctcgg cctcgcggag
     5221 cgcgccgagc cggtggccgc gggcgcgtga cggcatgacg cggcggcggg acgagccggt
     5281 ccggatggac cggatctaca cgcgcggcgg cgacgcgggg gagacgagcc tcggcgacgg
     5341 ggcgcgcgtg ccgaaggact cgccgcgcat cgaggcctac ggcgacgtcg acgagctgaa
     5401 cgccgtcctc gggctcgcgc tcgcgcgcgg gctgccggag cctgcagcct cgtggctcac
     5461 gatcgtccag aacgagctct tcgacctcgg cgccgacctc gccgtgccgc tcggggacgc
     5521 gcgcgagcgg ctccgcgtga cggccgggca ggtggagcgg ctcgaggcgt ggtgcgacga
     5581 ggcgaacgag gggctcccga tgctgcgctc gttcgtgctg cccggcggct cggaggccgc
     5641 ggcgctcctc catctcgggc ggaccgtgtg ccggcgcgcg gaacgccggg cggtgacgct
     5701 cgcggagcgc gaggaggtca atccccaggc gctcgcgtac ctgaaccggc tctccgacct
     5761 g
//
LOCUS       DASTFC010000193         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1585759,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000193 DASTFC010000000
VERSION     DASTFC010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585759"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFO88_05820"
     CDS             complement(<1..443)
                     /locus_tag="VFO88_05820"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9545075.1"
                     /translation="MLIAGNWKMHKTAGETIEFCTALRAAVEGLEGVEIVVCPPYTSL
                     DEAVRTLAGSGIHVFSQNAHWEPEGAFTGEVSAPMLAELGVVGSLVAHSERRQLFAET
                     NESAARRIAGVLDAGLRVIACVGETEQEREAGETEAVLTRQVEAIR"
     gene            complement(505..1554)
                     /locus_tag="VFO88_05825"
     CDS             complement(505..1554)
                     /locus_tag="VFO88_05825"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9545076.1"
                     /translation="MHRPRSVRDADVAGKRVLVRVDFNVPLEDGRVADDTRIRAALPT
                     LELLLERGAAELVLASHLGRPKGPDPAFSMEPVRVRLRELLPGGGERVRLLENTRFDP
                     REKANDPAFAEELAAHGDLFVNDAFGSAHRAHASTEGVARLLPAYAGLLLERELAELG
                     ALLESPERPFVAVVGGAKVEDKIGVLESLAGRADRLLVGGKMAEERKALAPLGEKLVL
                     PVDAVAAAAFEADAEAREVAVDDLPDGWLGLDIGPRTREAFTAEIAGARTVFWNGPMG
                     VFEWPRFAAGTRAIAEAVAACDGHTVVGGGDSVRAVDELGLADRIDWVSTGGGASLEL
                     LEGKELPGVAAIPAA"
     gene            complement(1554..2552)
                     /gene="gap"
                     /locus_tag="VFO88_05830"
     CDS             complement(1554..2552)
                     /gene="gap"
                     /locus_tag="VFO88_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9545077.1"
                     /translation="MSVKVGINGFGRIGRNFFRAARKQNADIEIVAVNDLGDAKTMAH
                     LLKYDSVLGPLGEDVEASEGAIRVGDAEIRLLNEKDPSALPWKDLGVEVALESTGIFT
                     KRDGAQKHLDAGAEKVVISAPATDPDVTLVLGVNDDEYDRENHRIVSNASCTTNCVAP
                     VAKVLHDAWGIQSGFMTTIHAYTNDQRILDLPHKDLRRARAAAINLIPTSTGAAKAIG
                     VVIPELKGVVDGVSIRAPVPTGSITDLVVTVGSEVTRDEVNARFEEVADSGPFEGILR
                     YSTDALVSSDIVGDSYSSILDADQTMAKGSMVKVMSWYDNEWGYSCRLVDLVQRLL"
     gene            complement(2806..3726)
                     /gene="whiA"
                     /locus_tag="VFO88_05835"
     CDS             complement(2806..3726)
                     /gene="whiA"
                     /locus_tag="VFO88_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET9545078.1"
                     /translation="MSLSEDARAELAAIEPRKACCRLAELSALVRSAGSVHLLGRGRI
                     ALHLELGSAGVARRAVALLHAYGVACEIRTFRRRAFDGATRYQLHLGDDQRSVEALTA
                     AGILGPGHRPLARPPARVTGRSCCRAAYLRGAFLAAGSVSGPRSAHLELRTVDLEGAR
                     QLARVAAPEGFTLGVLERRGHALAYAKGTETIADLLAFLGASDAALGLGEQAVVAATR
                     AQANRLANADHANLRRSSRAAEAQMRAIERLRNDGRLEHMRPELREIAELRLRHPTLP
                     LRDLAGRCRPATTKAAAHRRLAKLQRLADA"
     gene            complement(3723..>4159)
                     /locus_tag="VFO88_05840"
     CDS             complement(3723..>4159)
                     /locus_tag="VFO88_05840"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9545079.1"
                     /translation="VPGWGVGELWLDGALVVNHESPVARAGEENGASHPLVERVRAYF
                     GGARDAFADVELDLEACTPFQRAVVDALRAVPYGETVTYGELAALAGHPRAGRAAGTV
                     CAQNRFFLFVPCHRVVGAGSLGSYGSLGLGYKRRLLELEGAR"
BASE COUNT          558 a         1578 c         1498 g          525 t
ORIGIN      
        1 cggatcgcct cgacctggcg ggtgaggacg gcctccgtct cccccgcctc gcgctcctgc
       61 tcggtctcgc cgacgcaggc gatcacccgg aggcccgcgt cgagcacccc ggcgatgcga
      121 cgcgccgcgg actcgttcgt ctcggcgaag agctgccggc gctccgagtg cgcgacgagg
      181 gagccgacga cgccgagctc cgcgagcatc ggggcggaga cctcgccggt gaaggccccc
      241 tcgggctccc agtgggcgtt ctgggaaaag acgtggatcc cgctccccgc gagggtgcgc
      301 accgcctcgt cgagggacgt gtacggcggg cagacgacga tctcgacgcc ctcgaggccc
      361 tcgaccgccg cgcgcagcgc cgtgcagaac tcgatcgtct cgccggcggt cttgtgcatc
      421 ttccagttcc ccgcgatgag catggcgggc atcttacgag ccccgcccgc acggccacgg
      481 cgagcacgga cgcggcccgg cgcgctacgc ggcggggatc gccgcgacgc cggggagctc
      541 cttgccctcc aggagctcga gcgaggcgcc gccgccggtc gacacccagt cgatccggtc
      601 ggcgaggccg agctcgtcga cggcacgcac ggagtcgccg ccgccgacga ccgtgtggcc
      661 gtcgcacgcc gcgaccgcct ccgcgatcgc gcgcgttccg gcggcgaagc gcggccattc
      721 gaagacgccc atcggcccgt tccagaagac cgtgcgcgcg ccggcgatct ccgccgtgaa
      781 cgcctcgcgc gtgcgcggcc cgatgtcgag cccgagccag ccgtccggca ggtcgtcgac
      841 cgcgacctcg cgcgcctcgg cgtccgcctc gaacgccgcc gccgcgacgg cgtcgaccgg
      901 caggacgagc ttctcgccga gcggcgccag cgccttgcgc tcctcggcca tcttcccgcc
      961 gacgagcagc cggtcggcac gccccgcgag cgactcgagg acgccgatct tgtcctccac
     1021 cttcgccccg ccgacgaccg cgacgaaggg ccgctcgggc gactcgagca gcgcacccag
     1081 ctccgcgagc tcgcgctcca ggagcagccc cgcgtacgcc ggcagcagcc gggcgacgcc
     1141 ctccgtcgac gcgtgcgccc ggtgcgccga cccgaacgcg tcgttcacga agaggtcgcc
     1201 gtgcgcggcg agctcctccg cgaacgcggg gtcgttcgcc ttctcgcgcg gatcgaagcg
     1261 cgtgttctcg aggagccgga cgcgctcgcc gccgccgggg agcagctcgc gaagccgcac
     1321 ccgcaccggc tccatggaaa acgcgggatc gggccccttc ggccgcccga ggtgggacgc
     1381 cagcacgagc tccgcggccc ctcgctcgag gagcagctcg agcgtcggca gcgcggcccg
     1441 gatccgcgtg tcgtccgcca cccggccgtc ctcgagcggc acattgaagt cgacgcggac
     1501 gaggacgcgc ttccccgcga cgtccgcgtc ccgtaccgag cgcggccggt gcactagagg
     1561 agccgctgga cgagatccac gagccggcac gagtaccccc actcgttgtc gtaccagctc
     1621 atgaccttga ccatcgagcc cttcgccatc gtctggtcgg cgtcgaggat cgaggagtag
     1681 gaatcgccga cgatgtcgga cgagacgagc gcgtcggtcg agtaccggag gatcccctcg
     1741 aacggccccg agtcggccac ctcctcgaag cgcgcgttca cctcgtcccg cgtgacctcg
     1801 ctcccgaccg tcaccacgag gtcggtgatc gagcccgtcg ggacgggcgc ccggatcgag
     1861 acgccgtcca cgacgccctt gagctccggg atcacgaccc cgatcgcctt cgcggcgccg
     1921 gtcgaggtcg ggatgaggtt gatcgccgcc gcccgcgcgc gccggaggtc cttgtgcggg
     1981 aggtcgagga tccgctggtc gttcgtgtac gcgtggatcg tcgtcatgaa cccggactgg
     2041 atcccccacg cgtcgtggag gaccttcgcc acgggcgcga cgcagttcgt cgtgcaggag
     2101 gcgttcgaga cgatccggtg gttctcccgg tcgtactcgt cgtcgttcac gccgaggacg
     2161 agcgtcacgt ccgggtccgt cgcgggcgcc gagatgacga ccttctccgc acccgcgtcg
     2221 agatgcttct gcgccccgtc gcgcttcgtg aagatcccgg tcgactcgag cgcgacctcg
     2281 acgccgaggt ccttccaggg gagcgcggac gggtccttct cgttcaggag gcggatctcg
     2341 gcgtcgccga cgcggatcgc gccctcggag gcctcgacgt cctcgccgag cggcccgagg
     2401 acggagtcgt acttcaggag gtgcgccatc gtcttcgcgt cgcccaggtc gttcacggcg
     2461 acgatctcga tgtccgcgtt ctgcttgcgc gcggcgcgaa agaagttgcg cccgatccgc
     2521 ccgaacccgt tgatgcccac cttgacgctc atccggcctc cttcgtcgct tccccggcgc
     2581 ggcgagtgta accgtgaggc cgtgagacga gcgggccgga ggggaagctc cgaggacgac
     2641 ctccccctcc ggattcaacc gcgctccgga ccgtaggcgt cggacggggt ctgaatccaa
     2701 caccggacga gcgcggaccc gctcgatgca ggacattgac gcatggcgac gccggattca
     2761 aggcgaccgg gccagccgga ccccgcaaat tgcggccaga aactgtcaag cgtcggcgag
     2821 ccgctggagc ttcgcgaggc gccggtgggc cgccgccttc gtggttgccg gccggcagcg
     2881 gccggcaagg tcgcgaagcg ggagcgtcgg gtggcgcagg cggagctcgg cgatctcgcg
     2941 cagctcgggc cgcatgtgct cgagccggcc gtcgttccgg aggcgctcga tcgcgcgcat
     3001 ttgcgcctcg gccgcccggc tcgagcggcg gaggttcgcg tggtcggcgt tcgcgagccg
     3061 gttcgcctgc gcgcgcgtcg cggcgacgac cgcctgctcg ccgagcccga gcgccgcgtc
     3121 gctcgcgccg aggaacgcga gcaggtcggc gatcgtctcg gtgcccttcg cgtacgcgag
     3181 cgcgtggccg cgccgctcga ggacgccgag cgtgaacccc tccggggccg cgacacgcgc
     3241 gagctgccgc gcgccctcga ggtcgaccgt ccgcagctcg agatgcgccg agcgcggccc
     3301 cgagaccgag ccggcggcga ggaacgcccc gcggaggtac gcggcccggc agcacgagcg
     3361 gcccgtcacg cgggccggcg gccgggcgag cgggcggtgg ccggggccga ggatccccgc
     3421 cgccgtcagc gcctcgaccg agcgctggtc gtccccgaga tggagctggt agcgcgtcgc
     3481 cccgtcgaag gcgcgccggc ggaacgtccg gatctcgcac gcgacgccgt atgcgtgcag
     3541 gagcgcgacc gcgcggcggg cgacgccggc gctgccgagc tcgaggtgga gcgcgatccg
     3601 tccgcggccg aggaggtgga cgctcccggc cgagcggacg agcgccgaca gctccgcgag
     3661 ccggcagcac gccttgcgcg gctcgatcgc cgcgagctcg gcccgggcgt cttccgacag
     3721 gctcaccgcg cgccctccag ctcgagcagc cgccgcttgt agccgagccc gagggagccg
     3781 tacgagccga gcgagcccgc gccgacgacg cggtggcacg gcacgaagag gaagaagcgg
     3841 ttctgggcgc agaccgttcc ggccgcccgc ccggcgcgcg ggtgcccggc gagcgccgcg
     3901 agctcgccgt acgtgaccgt ctcgccgtac ggcaccgcgc ggagcgcgtc cacgaccgcc
     3961 cgctggaagg gcgtgcacgc ctcgaggtcg agctcgacgt ccgcgaacgc gtcccgcgcg
     4021 ccgccgaagt acgcgcgaac acgctcgacg agcgggtgcg aggcgccgtt ctcctccccg
     4081 gcccgcgcga cgggcgactc gtggttcacg acgagcgccc cgtcgagcca gagctccccg
     4141 acgccccacc ccggcacgg
//
LOCUS       DASTFC010000194         5762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1075288,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000194 DASTFC010000000
VERSION     DASTFC010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075288"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1138)
                     /locus_tag="VFO88_05845"
     CDS             complement(164..1138)
                     /locus_tag="VFO88_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9545080.1"
                     /translation="MGSTSGHSPLAIEATGLVKSFGETRAVDGVDLSVRDGSVYGVLG
                     PNGAGKTTTVRMLATLLRPDAGSARILGHDVVREADTVRGLVSLTGQLASVDEDLTGR
                     ENLILLGRLLGLRRPDAKARAAELLEAFGLADAAGRQVKHYSGGMRRRLDIAASIVVT
                     PRLMFLDEPTTGLDPRSRNQVWDIVRALIGEGTTVLLCTQYLEEADQLADRIAVIDHG
                     RVIAEGTPAQLKASVGRGALHVRLLDPGQRPRAAELLGDDVHLLPDPTALSLPGAGAE
                     QASEAIAALVAAGIGVAGFSLGQPSLDEVFLALTGHPAEDTIEEEAAA"
     gene            1361..2770
                     /locus_tag="VFO88_05850"
     CDS             1361..2770
                     /locus_tag="VFO88_05850"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HET9545081.1"
                     /translation="MSRLTPDPTFYPTATAAAAAEPERLGYVATIGVGENGSSRPDAL
                     AVLDLDPESETYGRIVSRVEMPNLGDELHHFGWNACSSSLCPWMPHPHVERRFLVLPG
                     LRSSRVYVVDTGDDPRNPRIVKTIEPEEIAEKTGYSRAHTVHCGPDGIYVNALGDPDG
                     DGPGGIFVLDHDTFEVKGAWERDRGTQQLAYDFWWHLGYDRVITSEWGTPNMVEDGVN
                     PELLLGNKYGHKLHVFDMKRRKHLQELDLGAEHQMVLELRPAHDPDKAYGFVGVVTST
                     ADLSASVFMWERLPDGTFAAEKVISIPAEPAEEEDLPPALKPFGAVPPLVTDIALSVD
                     DRSLYVSCWGTGEIKRYDVSDPRNPKETGSVRIGGIVANAPHPAAGPVNGGPQMVEVS
                     RDGRRVYLTNSLYRSWDEQFYPEGIRGWMVKLDAAEDGSLALDPGFFVGFPEGERPHQ
                     VRLEGGDASSDSYCFPNGN"
     gene            2770..3417
                     /locus_tag="VFO88_05855"
     CDS             2770..3417
                     /locus_tag="VFO88_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545082.1"
                     /translation="METLPLVTVALLGAYHGLNPAMGWLFAVGLGMQDRSRRSVLRAL
                     PPIAAGHELAIVVVAALVVGLGALADPAPLKVVGAVALIAFGIFRFARPRAHPRWTTM
                     RVTRKELTWWSFLMSSAHGAGLMVAPVLLGTGATETAHAHGGHASHSSSDVMGVADAA
                     VAVSVHVGAMLAVMAVVALLVYEKLGLTFLRRAWVNSDQFWAGAFVVAGVLTLVT"
     gene            complement(3410..>5762)
                     /locus_tag="VFO88_05860"
     CDS             complement(3410..>5762)
                     /locus_tag="VFO88_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545083.1"
                     /translation="PFAEVERAHLEELRLACREDRIDAELAAGRAAEVTGEVEALALR
                     HPLRERLHRQLILALYRAGRQAEALEAYDRFRRTLDEKLGLEPSPALKRLQQQILTQD
                     PSLEPPAARPETSAPRPARRRPAGLVGREAEVAQLETLLDAAARGEGSTVLVTGRAGI
                     GKSRLVAELAARAGERGATVLSGRCIQLVGVGLPYLPFAEALRPVAESPPLAELAGQL
                     HELARLVPELANGGSPVPPPEHGTDSRLRLFQEVFLALERLGAERPVVLALEDLHWAD
                     GSTLDLLAFLAHGVARAPVLVVGTYRTEEVAPGAALQRLAASIVGARAAVSLELEPLA
                     ADAVAALVRTDCTTPPPEELVDTIVSRSEGNPLFAQQLAAAAARGETALPPALRDMLL
                     ADVARLDANGRAVLRIAAAAGRDAPYDLLAAIAPLEELELAEALRQAVEQDLLVPDQE
                     AGTFRFRHALFAEAVYETLLPGEREVLHGRIARALTEEPQLAMGGAAGEAAQHWAAAR
                     RPAEALAASLQAAKEAERISGLAEALGYLERVLALWDEVPDAEALAGVALPAVLSRAA
                     ELASMSAESEDELDLRRVVGVLDFDEWVDVDTVAERLETSTATAERWLASLEHSGLVE
                     QRDDRFRAAPLAMNEARRLYPAAVVLESVAVRRSPRFGEEKLRALREANAALRAAAGD
                     AAAASAADDEFHRRLTADCGNEELIAALLPIKRALLRYELVYMDDPERVGRSADQHDE
                     IVAALERGDHAEAAQLLRGNLAHGLPDLQKAVEDGSGSAPAVT"
BASE COUNT          722 a         2135 c         2168 g          737 t
ORIGIN      
        1 cgcggcactg gtcgcgctga tccgcggcgg gcgcggggtg gacgacaggg cgctgcggat
       61 cgcggcctgg tcggtggaca tcgcgtcgac gctcatcggg caacctctcc gggggtccct
      121 ggaccccgcg ctcggtccct cgccagccgg ttgacagggg cgctcatgcc gcggcctcct
      181 cttcgatggt gtcctcggcg ggatggccgg tcagggcgag gaagacctcg tccaggctcg
      241 gctggccgag cgagaagccg gccacgccga tcccggcggc gacgagcgcc gcgatcgcct
      301 ccgacgcctg ctcggcgccc gcacccggca gcgagagcgc ggtcgggtcg ggcagcaggt
      361 gcacgtcgtc gccgagcagc tcggcggccc gcgggcgctg gccggggtcg agcaggcgca
      421 cgtgcagcgc gccgcgcccg accgacgcct tcagctgcgc cggcgtgccc tcggcgatca
      481 cgcgcccgtg gtcgatgacg gcgatccggt cggcgagctg gtcggcctcc tcgaggtact
      541 gcgtgcacag cagcacggtc gtgccctcgc cgatcagcgc gcgcacgatg tcccacacct
      601 ggttgcgcga gcgcgggtcc aggcccgtgg tcggctcgtc gaggaacatc agccggggcg
      661 tcacgacgat gctcgcggcg atgtccagcc gccggcgcat gccgcccgag tagtgcttga
      721 cctggcgccc ggcggcgtcg gcgaggccga acgcctcgag cagctcggcg gcgcgcgcct
      781 tcgcgtcggg gcgccgcagg ccgagcagcc ggccgagcag gatgaggttc tcgcggccgg
      841 tcaggtcctc gtcaaccgag gcgagctggc ccgtgaggct cacgagcccg cgcaccgtgt
      901 ccgcctcgcg cacgacgtcg tggccgagga tgcgcgccga gcccgcgtcg gggcgcagga
      961 gcgtcgcgag catgcgcacg gtcgtcgtct tgccggctcc gttcgggccg aggacgccgt
     1021 agaccgagcc gtcccgcacg gagaggtcga cgccgtcgac ggcgcgcgtc tcgccgaacg
     1081 acttcacgag gcccgtggcc tcgatcgcga gtggggagtg tccgctcgtg gagcccattc
     1141 tcgtccgtac agaccgcgcc ggtcgcgaga actcatcggt gcgctcgctc ggcctgtcgg
     1201 gcgagtatcg cgggcgccgc cggggtcgcg cgtggggttt cccgccccgc ggcggtacgg
     1261 gtagcacgag tgccgccgcg gccgtttacc ggatcgcaac cggacggcta ccggagggcg
     1321 ctcaaatcgg gcgaaaccga cccgaaggag gcaccaccga atgtcccgtc tcacccccga
     1381 cccaacgttc tacccgaccg ccacggcggc ggccgccgcg gagcccgagc ggctcggcta
     1441 cgtcgccacg atcggcgtcg gggagaacgg ctcctcgcgg cccgacgcgc tcgccgtcct
     1501 cgacctcgac cccgagtcgg agacgtacgg ccggatcgtc agccgcgtcg agatgccgaa
     1561 cctcggcgac gagctccacc acttcggctg gaacgcatgc tcgtcctcgc tctgtccgtg
     1621 gatgccgcac ccgcacgtcg agcggcgctt cctcgtgctc cccggcctgc gctcctcgcg
     1681 cgtgtacgtc gtggacacgg gcgacgaccc gcggaacccg cggatcgtca agacgatcga
     1741 gcccgaggag atcgccgaga agaccggcta cagccgcgcg cacaccgtcc actgcgggcc
     1801 cgacggcatc tacgtgaacg cgctcggcga ccccgacggc gacggccccg gcggcatctt
     1861 cgtcctcgac cacgacacgt tcgaggtcaa gggcgcctgg gagcgcgacc gcggcacgca
     1921 gcagctcgcg tacgacttct ggtggcacct cggctacgac cgcgtgatca cgagcgagtg
     1981 gggcacgccg aacatggtcg aggacggcgt gaaccccgag ctcctgctcg gcaacaagta
     2041 cggccacaag ctccacgtct tcgacatgaa gcggcggaag cacctgcagg agctcgacct
     2101 cggcgcggag caccagatgg tcctcgagct gcgccccgcg cacgacccgg acaaggcgta
     2161 cggcttcgtc ggcgtcgtca cctcgaccgc cgacctctcg gcgtccgtct tcatgtggga
     2221 gcggctcccg gacggcacct tcgccgcgga gaaggtcatc tcgatcccgg ccgagccggc
     2281 cgaggaggaa gaccttccgc ccgcgctcaa gccgttcggc gccgtgccgc cgctcgtgac
     2341 ggacatcgcg ctctcggtcg acgaccgctc gctctacgtc tcctgctggg gcacgggcga
     2401 gatcaagcgc tacgacgtga gcgacccgcg caacccgaag gagaccggct ccgtgcggat
     2461 cggcggcatc gtcgcgaacg cgccgcaccc tgccgcaggg cccgtgaacg gcggcccgca
     2521 gatggtcgag gtgagccgtg acggccgccg cgtctacctc acgaactcgc tctaccgctc
     2581 gtgggacgag cagttctacc cggaagggat ccgcggctgg atggtcaagc tcgacgcggc
     2641 cgaggacggc tcgctcgcgc tcgaccccgg cttcttcgtc gggttcccgg agggcgagcg
     2701 gccgcaccag gtacgcctcg agggcggcga cgcctcgtcc gactcctact gcttcccgaa
     2761 cggaaactga tggagaccct gcctctcgtc accgtcgcgc tcctcggcgc ctaccacggg
     2821 ctcaatcccg cgatgggctg gctgttcgcc gtcggcctcg ggatgcagga ccgcagccgg
     2881 cggtcggtgc tccgcgcgct gccgccgatc gcggccggcc acgagctcgc gatcgtcgtg
     2941 gtcgccgcgc tcgtggtcgg cctcggggcg ctcgccgacc cggccccgct gaaggtcgtc
     3001 ggggcggtcg cgctcatcgc cttcgggatc ttccgcttcg cccggccacg cgcgcacccg
     3061 cgctggacga cgatgcgggt gacccgcaag gagctcacct ggtggtcgtt cctcatgtcg
     3121 agcgcccacg gcgccggcct catggtcgcg cccgtcctcc tcgggacggg cgcgaccgag
     3181 acggcccacg cgcacggcgg gcacgcgagc cactcgtcct cggacgtgat gggcgtcgcg
     3241 gacgcggcgg tcgcggtctc cgtccacgtg ggcgcgatgc tcgccgtcat ggcggtcgtc
     3301 gcgctcctcg tctacgagaa gctcggcctc acgttcctcc gcagggcctg ggtgaactcg
     3361 gatcagttct gggcgggcgc cttcgtcgtc gcgggagtgc tcacgctcgt cacgtgacgg
     3421 cgggggcgct cccgctcccg tcctccacgg ccttctggag gtcggggagg ccgtgggcga
     3481 ggttgccgcg caggagctgg gccgcctcgg catggtcgcc gcgctcgagg gcggcgacga
     3541 tctcgtcgtg ctggtcggcc gagcgcccga cgcgctcggg gtcgtccatg tagacgagct
     3601 cgtagcgcag gagcgcccgc ttgatcggga ggagcgcggc gatcagctcc tcgttcccgc
     3661 agtccgcggt gaggcgccgg tggaactcgt cgtcggccgc gctcgccgcg gccgcgtcgc
     3721 cggcggccgc gcggagggcc gcgttcgcct cgcggagcgc gcggagcttc tcctccccga
     3781 agcggggcga gcggcgcacg gccacgctct cgaggacgac ggcggccggg tagaggcgcc
     3841 gcgcttcgtt catcgcgagc ggggcggcgc ggaagcggtc gtcgcgctgc tcgacgagcc
     3901 cggagtgctc gagcgacgcg agccagcgct cggccgtcgc cgtcgacgtc tccagccgct
     3961 cggcgaccgt gtccacgtcg acccactcgt cgaagtcgag cacgccgacg acgcgccgca
     4021 ggtcgagctc gtcctcgctc tcggccgaca tgctcgcgag ctccgcggcg cgcgagagga
     4081 cggccgggag cgcgacgccg gcgagcgcct cggcgtccgg cacctcgtcc cagagggcga
     4141 gaacgcgctc gaggtagccg agcgcctcgg cgagaccgga gatccgctcg gcctccttcg
     4201 ccgcctggag ggacgccgcg agcgcctcgg ccgggcggcg ggcggccgcc cagtgctgcg
     4261 cagcctcgcc ggcggcgccg cccatggcga gctgcggctc ctcggtgagc gcccgggcga
     4321 tccgcccgtg gagcacctcc cgctcgccgg ggaggagcgt ctcgtacacg gcctcggcga
     4381 agagcgcgtg gcggaagcgg aacgtcccgg cctcctggtc ggggacgagc aggtcctgct
     4441 cgaccgcctg gcggagcgcc tcggcgagct cgagctcctc gagcggcgcg atcgcggcga
     4501 ggaggtcgta cggcgcgtcg cgcccggcgg cggcggcgat ccggagcacc gcgcggccgt
     4561 tcgcgtcgag gcgggccacg tccgcgagga gcatgtcccg cagggccgga gggagcgcgg
     4621 tctcgccgcg cgccgccgca gccgcgagct gctgcgcgaa gagcgggttg ccctcggagc
     4681 gggagacgat cgtgtcgacg agctcctcgg gcggcggcgt cgtgcagtcc gtgcgaacga
     4741 gcgctgcgac cgcgtcggcc gcgagcggct cgagctcgag cgagaccgcg gcgcgcgcgc
     4801 cgacgatgct cgccgcgagc cgctggagcg ccgcgccggg ggcgacctcc tccgttcggt
     4861 acgtcccgac gacgagcacg ggcgcgcgtg cgacgccgtg cgcgaggaac gcgagcaggt
     4921 cgagcgtcga gccgtccgcc cagtgaaggt cctcgagcgc gagcacgacc gggcgctccg
     4981 ccccgagccg ctcgagcgcg aggaacacct cctggaagag ccggagccgc gagtccgtgc
     5041 cgtgctcggg gggcggcacg ggcgatccgc cgttcgcgag ctcggggacg aggcgggcga
     5101 gctcgtgcag ctgtccggcg agctccgcga gcggcggcga ctccgcgacg gggcgcagcg
     5161 cctcggcgaa cggcaggtac ggcagcccga cgccgacgag ctggatgcac cggcccgaga
     5221 gcacggtcgc tccgcgctcg cccgcccgcg cggcgagctc cgcgacgagg cgcgacttcc
     5281 cgatcccggc gcggccggtg acgagcacgg tgctcccctc gccgcgcgcg gccgcgtcga
     5341 ggagcgtctc gagctgcgcg acctccgcct cgcgcccgac gagcccggcc gggcggcggc
     5401 gggcagggcg cggcgccgac gtttccgggc gcgcggcggg cggctcgagc gacgggtcct
     5461 gcgtgaggat ctgctgctgg aggcgcttga gcgcgggcga gggctcgagc ccgagcttct
     5521 cgtcgagcgt ccggcggaag cggtcgtacg cctcgagcgc ctcggcctgg cggccggcgc
     5581 ggtagagggc gaggatgagc tgccggtgca gccgctcgcg cagcggatgg cgaagcgcga
     5641 gcgcctcgac ctcgccggtc acctcggcgg cgcgcccggc ggcgagctcc gcgtcgatcc
     5701 ggtcctcgcg gcaggcgagg cggagctcct cgaggtgcgc ccgctcgacc tcggcgaagg
     5761 gc
//
LOCUS       DASTFC010000195         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1585801,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000195 DASTFC010000000
VERSION     DASTFC010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585801"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFO88_05865"
     CDS             complement(<1..461)
                     /locus_tag="VFO88_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545084.1"
                     /translation="MEGPNETQTLWREYKRTGERALRDRLILTYAPLVKYVAGRLGSG
                     LPAHVEEGDLVSYGLLGLIGAIERFDPERDIKFETYAIARIKGSIIDELRSMDWVPRS
                     VRARARDIERSIAQLEAKLGRAPNDEEISTHLGISEEEFQDSLLEISRSSIA"
     gene            complement(500..667)
                     /locus_tag="VFO88_05870"
     CDS             complement(500..667)
                     /locus_tag="VFO88_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545085.1"
                     /translation="MRGRSPLGALFLLIAAGFAGVAVAAGVAGGAAWVIAVAAGLLAL
                     WMGELALRALR"
     gene            707..779
                     /locus_tag="VFO88_05875"
     tRNA            707..779
                     /locus_tag="VFO88_05875"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:740..742,aa:Trp,seq:cca)
     gene            961..1677
                     /locus_tag="VFO88_05880"
     CDS             961..1677
                     /locus_tag="VFO88_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET9545086.1"
                     /translation="MPSNQGVDAALEQLFSADSSIYQERGFQRRVGFGERPALINIDL
                     ANAWTRPGNPFSCDGMDVIIPGVQRLLAGARAKGVPVVYTTTAYAVTEGPNSDMGIWN
                     RKIPLEELALGSDAVAVDERIAPEPGEQVIVKKRASAFHGTHLSSFLTALRVDTVIIT
                     GVTMAGCVRHTTEDAIAEGFRPIVVRECVGDRVPGVVEWNLFDIDAKFGDVEPLERVL
                     DYLEGVTPWREAQPATASGG"
     gene            complement(1631..2944)
                     /locus_tag="VFO88_05885"
     CDS             complement(1631..2944)
                     /locus_tag="VFO88_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9545087.1"
                     /translation="MPGETEEVLIVGGGAIGAAVALELARRGARPVLVERGSALAAGC
                     SAGNAGLICPSHSAPLATPAALRQGLRWMLTPDSPFRLHPRPALVPWLLRFAAASTKR
                     RVEASTRLISELSQASLELHAGLAGEGLDTGFERRGVLNVYESEAGFAAGRKEAEANS
                     STGMRPRVLVEGDVLDVEPAVGPEVAGAVLYPDEAHCDPLRFVQAVGRAAAHAGAEIR
                     TGVEVLRVRRRDGRIAAVETSAGELRPGSVVLATGAWTPRLSRDLGLFVPVEGGKGYH
                     VDVASAPGDPRIPVFMQESRVIATPLEGRLRLAGTLELAGLDLGIDARRTEAIVAAAR
                     RVLPLLGAPRVLERWAGLRPCAPDGLPLIGRPAELENLILATGHAMMGLTLAPITGRL
                     VADLLAGEAPGHDVMPLAPDRFRPMLGRRSASRPRRWRAAPLARA"
     gene            3071..>3610
                     /locus_tag="VFO88_05890"
     CDS             3071..>3610
                     /locus_tag="VFO88_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545088.1"
                     /translation="MTPFLRTRGLVLATAGFLLATAVAAGLAFAGSDLLDDRRILIPL
                     LLVLGGTGAVALLGWATERARSAELVERHDDEQARLEAERTLERQDAQARAARLEAEL
                     DAAHAGFEADQAAQAEAQADLRARLEGLERALPEERARYETRLDEANEELLRERRALG
                     RSETARRAEREWASQIRRQL"
BASE COUNT          514 a         1326 c         1318 g          452 t
ORIGIN      
        1 gcgatcgagg accgcgagat ctcgagcagc gagtcctgga actcctcctc gctgatcccg
       61 aggtgcgtcg agatctcctc gtcgttcggc gcccggccga gcttcgcctc gagctgggcg
      121 atcgagcgct cgatgtcgcg cgcccgggcg cgcaccgacc gcggcaccca gtccatcgag
      181 cggagctcgt cgatgatcga gcccttgatc cgcgcgatcg cgtacgtctc gaacttgatg
      241 tcgcgctccg ggtcgaaccg ctcgatcgcg ccgatcaggc cgagcagccc gtaggagacg
      301 aggtcgccct cctccacgtg cgcgggcagc ccgctgccga ggcgaccggc gacgtacttg
      361 acgagcggcg cgtacgtgag gatcagcctg tcgcggagcg cgcgctcgcc cgtgcgcttg
      421 tactcgcgcc agagagtctg ggtctcgttc ggcccttcca ccggcacaag gatatgacgc
      481 gcggcggctc gcgctctcct cagcggaggg cgcggagggc gagctcgccc atccagagcg
      541 cgaggagccc ggccgcgacc gcgatcaccc aggccgcccc gccggcgacc ccggccgcga
      601 ccgcgacgcc cgcgaacccg gccgcgatga gcaggaacag cgccccgagc ggcgaccggc
      661 ctcgcatggg acggagtcta ggtcgcgtct acaatgagcc acgccgaggg ccgtagctca
      721 actggcagag caccggtctc caaaaccggg ggttgcaggt tcgagtcctg ccggccctgt
      781 ttcgccgatt cgagtccgcc ccgcgctgcg cgtccggagg acgacgtcac ggatgacggc
      841 gacgctcgcg tcgcgatcac cggcctccgt gctcctcgtc gattcggttc cggcatgctg
      901 cgtcaagcgc aagcggacgg gtctaccaac gctcgttcct cgacccgaag gaggattgtc
      961 atgccgtcga accaaggcgt cgacgcggcc ctcgagcagc tcttctcggc cgactcgtcg
     1021 atctaccagg aacgcggctt ccagcggcgc gtcggcttcg gcgagcgccc cgcgctcatc
     1081 aacatcgacc tcgccaacgc ctggacgcgt ccggggaacc ccttcagctg cgacgggatg
     1141 gacgtcatca tccccggggt ccagcgcctg ctcgcggggg ctcgtgcgaa gggcgtcccc
     1201 gtcgtctaca cgacgacggc ctacgccgtg accgagggac cgaacagcga catggggatc
     1261 tggaaccgca agatcccgct cgaggagctc gcgctcggtt cggacgccgt cgcggtcgac
     1321 gagcgaatcg cgcccgagcc gggcgagcag gtcatcgtca agaagcgagc cagcgccttc
     1381 cacgggacgc acctgtcgag cttcctcacc gcgctccgcg tggacacggt gatcatcacc
     1441 ggcgtgacga tggccgggtg cgttcggcac acgacggagg acgcgatcgc cgagggcttc
     1501 cgtccgatcg tcgtccgtga atgcgtcggc gaccgcgtcc ccggcgtcgt cgagtggaac
     1561 ctgttcgaca tcgacgcgaa gttcggcgac gtcgagccgc tcgagcgcgt cctcgactac
     1621 ctcgagggcg tcacgccctg gcgagaggcg cagcccgcca ccgcctcggg cggctagcgg
     1681 accggcgtcc aagcatcggc cggaaccggt cgggcgcgag gggcatgacg tcgtggccgg
     1741 gcgcctcacc cgcgagcagg tcggcgacca agcggccggt gatgggcgcc agcgtcaggc
     1801 ccatcatggc gtgcccggtg gccaggatca ggttctcgag ctcggccggg cgaccgatga
     1861 gcgggaggcc gtcgggcgcg cacgggcgca ggccggccca acgctcgagc acgcgtgggg
     1921 cgccgagcag ggggaggacg cggcgcgcgg cggcgacgat cgcctcggtg cggcgggcgt
     1981 cgatgccgag atcgagcccg gcgagctcga gcgtgccggc caggcggagg cgtccctcga
     2041 gcggggtcgc gatcacccga gactcctgca tgaagacggg tatccgcggg tcgcccggcg
     2101 ccgacgcgac gtcgacgtgg taccccttgc cgccctcgac ggggacgaac aagccgaggt
     2161 cgcggctcaa ccgcggcgtc cacgccccgg tggccagtac gaccgagccg ggccgcagct
     2221 ccccggcgct cgtctcgacc gcggcgatac ggccgtcgcg gcggcgtact cggaggacct
     2281 ccacccccgt acggatctcg gcccccgcgt gggctgcggc gcgcccgacc gcctgcacga
     2341 agcggagcgg gtcgcagtgt gcctcgtccg ggtagaggac ggcgccggcg acctcgggac
     2401 cgaccgccgg ctcgacgtcc agaacgtcgc cctcgacgag gacgcgcggg cgcatccccg
     2461 tggacgagtt cgcctcggcc tccttccggc cggccgcgaa acccgcctcg ctctcgtaga
     2521 cgttcagcac accgcgccgc tcgaagccgg tgtcgagccc ctcgccggcg agcccggcgt
     2581 ggagctcgag gctcgcctgg ctgagctcgc tgatgagacg cgtcgacgcc tcgacccgcc
     2641 gcttcgtgct ggccgcggcg aagcggagaa gccagggcac cagggcgggc ctcgggtgga
     2701 ggcggaacgg gctgtcgggc gtgagcatcc agcggaggcc ctggcggagc gccgccggcg
     2761 tcgcgagcgg agccgagtgg ctcgggcaga tgaggccggc gttgcccgcc gagcaaccgg
     2821 cggcgagcgc agagccccgc tccacgagga ccggcctggc gccgcggcgc gcgagctcga
     2881 gcgcgaccgc cgcgccgatc gcgccgcccc ccacgatcag aacctcctcc gtctcccccg
     2941 gcaccggctc gatggtactc acgccggacg aaacgcccac cgaccgcaag tcgggggacg
     3001 gcgacggcgg gacccaactc ccgcaggcag gaatcccacg cccgctccgg tacaccgagg
     3061 agctctgccg atgacgccgt ttctccgcac ccggggcctc gttctcgcga cggccggctt
     3121 ccttctcgcg acggccgttg cggccgggct cgcgttcgcc ggctcggatc tgctcgacga
     3181 tcgcaggatc ctgatcccgc tgctgctcgt tctaggcggc acgggtgcgg tcgccctcct
     3241 cgggtgggcg acggagcgcg cccgctcggc tgagctcgtc gagcgccacg acgacgagca
     3301 ggcccggctc gaggcggagc ggacgctcga gcggcaggat gcgcaggcgc gcgcggcccg
     3361 gctcgaggcg gagctcgacg cggcgcacgc ggggttcgag gccgaccagg ctgcgcaggc
     3421 cgaggcccag gcggacctgc gcgcccggct cgaggggctc gagcgggcgc tgccggagga
     3481 gcgcgcccgc tacgagacgc ggctcgacga ggcgaacgag gagcttcttc gcgagcggcg
     3541 cgccctcggg cgcagcgaga cggcccggcg cgccgagcgg gagtgggcgt cgcagatccg
     3601 gcggcagctc
//
LOCUS       DASTFC010000196         3722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_762069,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000196 DASTFC010000000
VERSION     DASTFC010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762069"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1673)
                     /locus_tag="VFO88_05895"
     CDS             complement(288..1673)
                     /locus_tag="VFO88_05895"
                     /inference="COORDINATES: protein motif:HMM:NF005102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9545089.1"
                     /translation="MSTPATSLSPAELQRAAHDHLWLHFTRMGGYAESDIPIIVRGDG
                     CYLEDAHGKRYLDALAGLFAVQVGYSHGEEIGEAAAAQMRELPFYTNWSYAHPRAIEL
                     AAEVASLAPGDLNRVFFCSGGSEAVESAWKLARQYHAARGERRWKAISRRVAYHGTTM
                     GALSINGIAALRNPFEPLVPDVLHVRNTNRYHRPPEESEEDFTAFLLEDLESTIEAAG
                     PGTVAMVIMEPVQNAGGALTPPEGYWAGVREICDRHGILLCADEVITGFGRLGAWFGS
                     ERYDIRPDIVTSAKGLSSAYAAIGAVVARDAVMEPFLEGSAMYAHGITFGGHPVQAAI
                     ALANIEIMKRERIVEGVAERQDAFRATLAQLLDLPIAGDLRGTGYFYALELVKDKETR
                     ESFSPEECETLLRGYLSHALFEHGLICRADDRGDPVVQISPPLVAGQAEFDEIAGILG
                     DVLAEAATRLG"
     gene            complement(1694..2548)
                     /locus_tag="VFO88_05900"
     CDS             complement(1694..2548)
                     /locus_tag="VFO88_05900"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9545090.1"
                     /translation="MTSAAETVAAPRRSGPGAATRAWRVVRHHALTVYALLAFAYLML
                     PILVVVVFSFNDPIGRFNFTWQTFTLDNWLNPFAYPGLRDAVMTSLQIAALSSIVATI
                     LGTLIGLALVRYQFRGGGTTNFLIFLPMTTPEIVLGASLLTLFLNFGVPLGFGTILLA
                     HIMFNISYVVVTVRARLVGFDRHLEEAAMDLGANELVTFRRVTLPLIAPGILAAALLA
                     FALSVDDFVITYLNAGAVVTFPLFVWGAARVAVPPQINVIGTAIFLVALLLMLANILW
                     QWRRSGRS"
     gene            complement(2545..3402)
                     /locus_tag="VFO88_05905"
     CDS             complement(2545..3402)
                     /locus_tag="VFO88_05905"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9545091.1"
                     /translation="MGFIHRHKGLVPYLLLLPGIAFLAVFFLVPIYYLANTSLQSGDI
                     FSGYVFTWAWENYSNAISTYHPQLLRSFQYAGIATIVALLVSYPLAYWIAFRGGRWKN
                     LLLLLIIAPFFVTYLIRTLAWKTILEDDGLVVSAFRTVGLLGDDGRLLATSVAVVAGI
                     TYNFLPFMALPIYVSLEQVDERLVEAAQDLYASKARAFLRVTLPLSLPGVFAGTLLTF
                     IPAAGDFINAQLLGTPRQYMIGNVIQSKFKVIADYPEAAALSFILMATILVLLVAYVR
                     ALGTERLSV"
     gene            complement(3431..>3722)
                     /locus_tag="VFO88_05910"
     CDS             complement(3431..>3722)
                     /locus_tag="VFO88_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545092.1"
                     /translation="IWTDNMLIPKGGDVFTASTFMDFVYRPEIAAQIEAYVNFICPVE
                     GAREAMLELDPEIADNPLIFPTEEMLANVNQFDTEAADDQEFKQTFQTVTGA"
BASE COUNT          617 a         1232 c         1340 g          533 t
ORIGIN      
        1 ccgccgccct cctgcgcgcc ggtgtccccg tccgtgcctg tctgcgcggc ctccccgttt
       61 ccgttgccgt tctcgtcgtc cgaatggaag cacccggccg cgagcagcga cacgaccacg
      121 agcaccaggc ccgtgatgat cagacgcctt ttgggcatcg cgcccctccc ttcagctagc
      181 cgtacgaatg tgacgcggag gagaacctaa cccagatgtc gccgccgcct caaaccttct
      241 gacatcgccg actcgccaaa agttggcgcc gctcggcgcc gtccggctca gccgaggcga
      301 gtcgcggcct ccgcgaggac gtccccgagg atgcccgcga tctcgtcgaa ctcggcctgg
      361 ccggcgacga gcggcggcga gatctggacg acgggatcgc cccggtcgtc ggcccggcag
      421 atgagcccgt gctcgaagag cgcgtgcgag aggtagccgc gcaggagcgt ctcgcactcc
      481 tccggggaga acgactcgcg tgtctccttg tccttcacga gctcgagcgc gtagaagtag
      541 ccggtgccgc gcaggtcgcc ggcgatcggg aggtcgagga gctgcgcgag cgtcgcccgg
      601 aaggcgtcct gccgctcggc gacgccctcg acgatccgct cccgcttcat gatctcgatg
      661 ttcgcaaggg cgatcgccgc ctggacgggg tggccgccga acgtgatccc gtgcgcgtac
      721 atcgcgctgc cctcgaggaa cggctccatc acggcgtcgc gcgcgacgac cgcgccgatc
      781 gccgcgtacg cggaggagag gcccttcgcc gacgtgacga tgtcgggccg gatgtcgtag
      841 cgctccgacc cgaaccacgc gccgagccgc ccgaagccgg tgatcacctc gtccgcgcag
      901 aggaggatgc cgtggcggtc gcagatctcg cgcacgccgg cccagtagcc ctccggcggc
      961 gtgagcgccc cgcccgcgtt ctggacgggc tccatgatca ccatcgcgac cgtgcccggc
     1021 ccggccgcct cgatcgtcga ctcgaggtcc tcgagcagga aggcggtgaa gtcctcctcg
     1081 ctctcctccg gcggccggtg gtagcggttc gtgttgcgga cgtggaggac gtccgggacg
     1141 agcggctcga acgggttgcg gagcgcggcg atcccgttga tcgagagcgc gcccatcgtc
     1201 gtcccgtggt acgcgacccg gcgcgagatc gccttccagc gccgctcccc gcgcgccgcg
     1261 tggtactgcc gcgcgagctt ccaggccgac tcgaccgcct cggagccgcc cgagcagaag
     1321 aagacgcggt tcaggtcgcc cggcgcgagc gacgccacct cggcggcgag ctcgatcgcg
     1381 cgcgggtgcg cgtacgacca gttggtgtaa aagggcagct cgcgcatctg cgcggcggcc
     1441 gcctcgccga tctcctcccc gtgcgagtag ccgacctgga cggcgaagag gcccgcgagc
     1501 gcgtcgaggt agcgcttgcc gtgagcgtcc tcgaggtagc agccgtcgcc gcggacgatg
     1561 atcgggatgt cgctctcggc gtagccgccc atccgcgtga agtggagcca gaggtggtcg
     1621 tgggcggccc gctgcagctc ggccggggac agggatgtcg cgggtgtgct catgggcatt
     1681 cctttcgggg ggttcagctc cggccggacc ggcgccactg ccagaggatg ttcgcgagca
     1741 tgaggaggag ggcgacgagg aagatcgccg tcccgatcac gttgatctgc ggcggcacgg
     1801 cgacccgtgc ggcgccccaa acgaagagcg ggaacgtgac gaccgcgccg gcgttcaggt
     1861 acgtgatcac gaagtcgtcc acggagagcg cgaacgcgag gagcgccgcc gcgaggatcc
     1921 ccggcgcgat caggggaagg gtgacgcgcc ggaacgtgac gagctcgttc gcgccgaggt
     1981 ccatggcggc ctcctcgagg tgccggtcga acccgacgag ccgggcgcgg acggtcacga
     2041 cgacgtacga gatgttgaac atgatgtggg cgagcaggat cgtcccgaac ccgagcggga
     2101 cgccgaagtt gaggaagagc gtgaggagcg aggcgccgag cacgatctcc ggggtcgtca
     2161 tcggcaggaa gatgaggaag ttcgtcgtcc cgccgccgcg gaactggtag cggaccagcg
     2221 cgagcccgat cagggtgccg aggatcgtgg ccacgatcga cgagagcgcc gcgatctgga
     2281 ggctcgtcat caccgcgtcc cgcaggcccg ggtacgcgaa cgggttcagc cagttgtcga
     2341 gcgtgaaggt ctgccaggtg aagttgaagc ggccgatcgg gtcattgaac gagaagacga
     2401 cgacgacgag gatcggcagc atcaggtacg cgaacgcgag gagcgcgtac acggtcaggg
     2461 cgtgatggcg caccacgcgc cacgcccggg tcgccgcacc cggcccggag cgccgcgggg
     2521 cggcgacggt ctcggcggcg ctcgtcatac gctcagccgc tccgtcccga gcgcgcggac
     2581 gtacgcgacc aggagcacga ggatcgtggc catgaggatg aaggagaggg ccgccgcctc
     2641 cggatagtcg gcgatcacct tgaatttcga ctggatgacg ttcccgatca tgtactgccg
     2701 cggcgtcccg aggagctgcg cgttgatgaa gtcgcccgcg gccgggatga acgtgaggag
     2761 cgtcccggca aagacgcccg ggagcgagag cgggagcgtg acgcggagga acgcgcgcgc
     2821 cttgctcgcg tagagatcct gcgccgcctc cacgagccgc tcgtcgacct gctcgaggct
     2881 cacgtagatc ggcagggcca tgaagggcag gaagttgtac gtgatccccg ccaccacggc
     2941 gaccgaggtg gcgagcaggc gcccgtcgtc gccgagcagg cccaccgtac ggaaggcgga
     3001 gacgacgagg ccgtcgtcct cgaggatcgt cttccacgcg agcgtccgga tgaggtacgt
     3061 gacgaagaac ggcgcgatga tgaggaggag caggaggttc ttccagcgcc cgccccggaa
     3121 ggcgatccag tacgcgagcg ggtagctgac gaggagcgcg acgatcgtcg cgatccccgc
     3181 atactggaac gagcgcagga gctgcgggtg gtacgtggag atcgcgttcg agtagttctc
     3241 ccacgcccag gtgaagacgt agccgctgaa gatgtcgccc gactggagcg acgtgttcgc
     3301 gaggtagtag atcgggacga ggaagaagac cgcgaggaac gcgatccccg gcaggagcag
     3361 caggtagggc accaggccct tgtgccggtg gatgaacccc atcgcctgcc cttcgccgcc
     3421 ccgacgcctg ctacgcgcct gtcaccgtct ggaacgtctg cttgaactcc tggtcgtccg
     3481 cggcctcggt gtcgaactgg ttcacgttcg cgagcatctc ctcggtcggg aagatgagcg
     3541 ggttgtccgc gatctccggg tcgagctcga gcatcgcctc acgcgcgccc tcgaccgggc
     3601 agatgaagtt cacgtacgcc tcgatctgcg ccgcgatctc cggccggtag acgaagtcca
     3661 tgaacgtcga cgcggtgaag acgtcgccgc ccttcgggat gagcatgttg tccgtccaga
     3721 tc
//
LOCUS       DASTFC010000197         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_795124,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000197 DASTFC010000000
VERSION     DASTFC010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795124"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /gene="glgP"
                     /locus_tag="VFO88_05915"
     CDS             complement(<1..1434)
                     /gene="glgP"
                     /locus_tag="VFO88_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET9545093.1"
                     /translation="MPSDGLLVAYFSLEFGVDERLPVYAGGLGVLAGDHLKAASDLGV
                     PLVGVGLFYREGYFRQEVGADGRQAERYEARDPAGLGLADTGVAVTVELPAGPVEARA
                     WRTDVGRVPLVLLDADGLSRALYGGDREHRLGQELLLGLGGVRALAALGLAPTVYHLN
                     EGHAAFTALERLRALVEDDGLAVEDALEAVRRTTVFTTHTPVPAGNERFDAELVRRHV
                     EGLAERIGLGWEGLAALGRADGDEAAFGLTPFALRTATRRNGVSRLHGRVARGMWAGL
                     WPERAADDVPIGHVTNGVHPRTWVSEELARLLEAQGVGLDRGEGWERAERLDLGELGR
                     ARRAGKEALLARVGALDPDALTLGFARRFATYKRANLLLSDPERFAALLADADRPVQL
                     LVAGKAHPADGGGKEIIEQVVAFARSGLAGGRVVFLADYDMALARALVQGVDVWVNVP
                     RRLEEASGTSGMKAGLNGALNLSVADGW"
     gene            1605..2186
                     /locus_tag="VFO88_05920"
     CDS             1605..2186
                     /locus_tag="VFO88_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET9545094.1"
                     /translation="MFQVRIHGRGGQGVVTAAELLSVAAFAEGRHAQAFPTFGSERTG
                     APVVGFCRMDERPIRIREPIAEPDALIVQDPTLLHQVDVFAGLRPDGYLLINSERGFE
                     ELGIGELCERFRPERLRTVPATEIARECLGRPLPNAALLGGFAALTGRVSLESVAKAI
                     RERFPGPVGERNVEAATRAHALVAGAEREPARA"
     gene            2179..3438
                     /locus_tag="VFO88_05925"
     CDS             2179..3438
                     /locus_tag="VFO88_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9545095.1"
                     /translation="MRSQLEGSQAVARTVARCRPEVVSAYPISPQTHIVEGLSNLVRT
                     GELAPCEYLLVESEFAAMSAAIGASATGARAYTATASQGLLYMAEAVYNAAGLGLPIV
                     MTVANRAIGAPINIWNDHSDTMSQRDCGWIQLYADSNQEAVDLHVQAFRLAEEVSLPV
                     MVCMDGFILTHAVEEIDVPEQEQVDAFLPPFEPRQVLDPDDPVTIGAMVGPEAFTEVK
                     YLMHAKQMQALDLIPEIAAEFSQAFGRSAGGLLRQYRTSDAETIVVVLGSVLGTVEEV
                     VDELRERGVRIGAVGIRCFRPYPLDELREALAQARRVVVLEKAFAVGVGGIVGQNVRL
                     ALSGLPVEVLDVVAGLGGRAITKRSLHGLLADALEGRLEEGRLTFLDLDRELVGRELA
                     RMGSGGRTGPHAENILRDVGAVAAGSH"
     gene            3449..>4305
                     /locus_tag="VFO88_05930"
     CDS             3449..>4305
                     /locus_tag="VFO88_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9545096.1"
                     /translation="MPQQQIKFYQAGSFVVGNRLLDPDERSVQARTGRSNTLTSGHRA
                     CQGCGEALGARYALDAAMRATGGRLIAANATGCLEVFSTPYPESSWQLPWIHSLFANA
                     PSVATGIAAALRAKGKTDIRVVGQGGDGGTMDIGFAALSGMFERNDDVLYVCYDNEAY
                     MNTGVQRSSATPPAARTATTGAVGPEPGNVFGQGKNAPLIAMAHEIPYVATATVAELR
                     DLEYKVERAMELHGARYLHVFVPCPLGWGSAARDTIKIARLAKETGLFPVFEAEHGEV
                     VDVSKIRRQV"
BASE COUNT          575 a         1611 c         1588 g          531 t
ORIGIN      
        1 ccagccgtcc gcgacggaga ggttcagcgc cccgttcagg cccgccttca tcccgctcgt
       61 ccccgaggcc tcctcgagcc ggcgcgggac gttgacccac acgtccacgc cctgcacgag
      121 cgcgcgggcg agcgccatgt catagtccgc gaggaagacg acgcggccgc cggcgagccc
      181 gctccgggcg aacgcgacca cctgctcgat gatctccttc ccgcccccgt cggccgggtg
      241 cgccttcccc gcgacgagga gctggacggg ccggtcggcg tccgcgagga gcgccgcgaa
      301 ccgctccggg tcggagagca ggaggttcgc gcgcttgtac gtcgcgaagc ggcgggcgaa
      361 gccgagcgtg agcgcgtccg ggtcgagcgc gcctacccgg gcgagcagcg cctccttgcc
      421 ggcgcgccgc gcccggccga gctcgccgag gtcgagccgc tccgcgcgct cccagccctc
      481 gccgcggtcg aggccgacgc cctgcgcctc gagcaggcgc gcgagctcct ccgagaccca
      541 cgtgcgcggg tggacgccgt tcgtgacgtg gccgatcggg acgtcgtcgg cggcgcgctc
      601 gggccagagg ccggcccaca tcccgcgcgc gacccggccg tgcaggcgcg agacgccgtt
      661 gcggcgcgtg gccgtccgca gggcgaacgg cgtgaggccg aacgccgcct cgtccccgtc
      721 ggcccgcccg agcgcggcga gcccctccca gccgagcccg atccgctcgg cgagcccctc
      781 gacgtggcgc cgcacgagct ccgcgtcgaa gcgctcgttc ccggccggca ccggcgtgtg
      841 cgtcgtgaag acggtcgtcc gccgcacggc ctcgagcgcg tcctcgaccg cgagcccgtc
      901 gtcctcgacg agggcgcgca gccgctcgag cgccgtgaac gccgcgtggc cctcgttcag
      961 gtggtagacg gtcggcgcga gcccgagcgc cgcgagcgcg cggacgccgc cgagcccgag
     1021 caggagctcc tggccgagcc ggtgctcccg gtcgccgccg tagagcgcgc gcgagaggcc
     1081 gtccgcgtcg aggaggacga gcgggacgcg gccgacgtcc gtccgccacg cgcgcgcctc
     1141 gacgggcccg gccggcagct ccacggtgac ggcgacgccg gtgtccgcga gcccgagccc
     1201 ggccgggtcg cgcgcctcgt agcgctcggc ctggcggccg tccgcgccga cctcctgccg
     1261 gaagtacccc tcccggtaga agagcccgac gccgacaagg ggcactccga ggtcggacgc
     1321 cgccttcagg tgatcgccgg cgagcacgcc gagcccgccc gcgtagacgg gcaggcgctc
     1381 gtccacgccg aactcgagcg agaagtaggc gacgaggagg ccgtcggaag gcaccgcgag
     1441 aggctaccga gccgagccgg ccggaatgcg ggaaagccgc acgcgattac cgggccgacc
     1501 ccgacggcgc gggagtcccc cgcgacgacc atgcgccgta gggcgatggg acgcacgacc
     1561 gttcagctca gatgcgaccc cggcgcgcgg aaggcgggcg cctgatgttc caggtgcgca
     1621 tccacggccg cggcggccag ggcgtcgtga cggcggccga gctcctctcc gtcgccgcgt
     1681 tcgccgaggg ccgccacgcg caggcgttcc cgaccttcgg ctcggagcgc accggcgcgc
     1741 ccgtcgtcgg cttctgccgg atggacgagc ggccgatccg catccgcgag ccgatcgccg
     1801 agccggacgc gctcatcgtc caggacccga cgcttctcca tcaggtggac gtcttcgccg
     1861 ggctgcgccc ggacggctac ctcctgatca actcggagcg cggcttcgag gagctcggga
     1921 tcggcgagct ctgcgagcgc ttccgtcccg agcgcctgcg gacggtcccg gcgaccgaga
     1981 tcgcgcgcga gtgcctcggg cggccgctgc cgaacgcggc gctgctcggc ggcttcgccg
     2041 cgctgaccgg ccgcgtctcg ctcgagtcgg tcgccaaggc gatccgagag cggttcccgg
     2101 ggcccgtcgg cgagcgcaac gtcgaggccg cgacccgggc gcacgcgctc gtcgcgggcg
     2161 ccgagcggga gccggcccgt gcgtagccag ctcgaaggat cccaggccgt cgcgcggacg
     2221 gtcgcgcgct gccggcccga ggtcgtctcg gcgtatccga tctcgccgca gacgcacatc
     2281 gtcgaagggc tatccaatct cgtgcggacg ggcgagctcg cgccgtgcga gtacctcctc
     2341 gtcgagtccg agttcgccgc gatgtcggcg gcgatcgggg cctccgcgac gggcgcgcgc
     2401 gcctacacgg cgacggcgag ccaggggctc ctctacatgg ccgaggcggt ctacaacgcc
     2461 gccgggctcg ggctcccgat cgtcatgacg gtcgcgaacc gggcgatcgg cgcgccgatc
     2521 aacatctgga acgaccacag cgacacgatg tcccagcgcg actgcggctg gatccagctc
     2581 tacgccgact cgaaccagga ggcggtcgac cttcacgtcc aggccttccg gctcgcggag
     2641 gaggtgtcgc ttcccgtcat ggtctgcatg gacgggttca tcctcacgca cgcggtcgag
     2701 gagatcgacg tgccggagca ggagcaggtg gacgcgttcc tcccgccgtt cgagccccgc
     2761 caggtgctcg accccgacga ccccgtgacg atcggggcga tggtcggccc cgaggcgttc
     2821 accgaggtga agtacctcat gcatgcgaag cagatgcagg cgctcgacct gatccccgag
     2881 atcgcggccg agttctcgca ggcgttcggc cgcagcgccg gcgggctgct ccggcagtac
     2941 cggacgagcg acgcggagac gatcgtcgtc gtgctcggct ccgtgctcgg cacggtcgag
     3001 gaggtcgtgg acgagctgcg cgagcggggc gtgcggatcg gcgccgtcgg gatccgctgc
     3061 ttccgcccct acccgctcga cgagctgcgc gaggcgctcg cgcaggcgcg ccgcgtcgtc
     3121 gtgctcgaga aggcgttcgc ggtcggggtg ggcgggatcg tcgggcagaa cgtccgcctc
     3181 gcgctctccg gcctgccggt cgaggtgctc gacgtcgtcg cgggcctcgg cgggcgcgcg
     3241 atcacgaagc gctcgctcca cgggctcctg gcggacgcgc tcgaaggccg cctggaggag
     3301 ggccggctca cgttcctcga cctcgaccgc gagctcgtcg gccgcgagct cgcccgcatg
     3361 ggcagcggcg gccgcacggg cccgcatgcc gagaacatcc tgcgcgacgt gggcgccgtc
     3421 gcggccgggt cgcactaggg aggtcgccgt gccgcagcag cagatcaagt tctaccaagc
     3481 gggaagcttc gtggtcggca accggcttct cgacccggac gagcgttccg tccaggcccg
     3541 gaccggccgc tcgaacaccc tcacgtcggg ccaccgcgcg tgccagggct gcggcgaggc
     3601 gctcggcgcc cgctacgcgc tcgacgccgc gatgcgcgcg accggcggca ggctcatcgc
     3661 cgcgaacgcg accgggtgcc tcgaggtctt ctccacgccg tacccggagt cgtcgtggca
     3721 gctcccgtgg atccactcgc tcttcgcgaa cgcgccgtcg gtcgcgacgg gaatcgccgc
     3781 cgcgctcagg gcgaagggga agaccgacat ccgggtcgtc ggccagggcg gcgacggcgg
     3841 gacgatggac atcggcttcg ccgccctctc cgggatgttc gagcgaaacg acgacgtgct
     3901 ctacgtctgc tacgacaacg aggcgtacat gaacacgggc gtccagcgct cgagcgcgac
     3961 gccgcccgcg gcgcgcacgg cgacgaccgg cgccgtcggc ccggagccgg ggaacgtctt
     4021 cggccagggg aagaacgcgc cgctcatcgc gatggcgcac gagatcccgt acgtcgccac
     4081 ggcgaccgtc gccgagctcc gcgacctcga gtacaaggtc gagcgcgcga tggagctcca
     4141 cggggcgcgc tacctccacg tgttcgtccc gtgcccgctc gggtggggct cggccgcgcg
     4201 cgacacgatc aagatcgccc gcctcgcgaa ggagacgggg ctcttcccgg tcttcgaggc
     4261 cgagcacggc gaggtcgtgg acgtctcgaa gatcaggcgg caggt
//
LOCUS       DASTFC010000198         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_102836,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000198 DASTFC010000000
VERSION     DASTFC010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102836"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..639
                     /locus_tag="VFO88_05935"
     CDS             73..639
                     /locus_tag="VFO88_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9545097.1"
                     /translation="MGKLVVTEFVSLDGVMQAPGGEDFKYPGWSFAFDRGDDGNQFKL
                     DETLETEALLLGRVTYESFAGAWPEREGEFADKFNTMPKYVVSSTLKDPEWNNTTVVD
                     GDVVGEAAKLKERTDGILQTPGSLRLVQTLLEHDLVDELHLMVFPVILGTGRRLFGET
                     TEKTDWRLTEARPVGPDGVLVLIYERAR"
     gene            complement(680..1660)
                     /locus_tag="VFO88_05940"
     CDS             complement(680..1660)
                     /locus_tag="VFO88_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9545098.1"
                     /translation="MRVFVAGASGALGSRLVPQLIDAGHEVIGTHRSPSRAAHVWTLG
                     AKPVRLDLLDAEAVRRAVLESEPEAIVHQATALADVKLGRDFDKVFGPTNALRTKGTD
                     ALLAAAREAGVPRFVAQSFASYRHAREGGPVKTEDDPLDPDPPRKTEESTGAMTYLEQ
                     AVTGFGGIVLRYGGFYGAANDGLIEPVRKRQFPIVGEGGGMFSWIHLDDAAAATVLAL
                     EHDGPAIYNVVDDEPAPVREWLPVLAEALDAKPPRRIPAWLARLVAGEAAVVLGTEAR
                     GASNAKARRELGWAPRYPSWRRGFAAAYSAMRTADRPEPHPAARTRQSPG"
     gene            1746..2624
                     /locus_tag="VFO88_05945"
     CDS             1746..2624
                     /locus_tag="VFO88_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545099.1"
                     /translation="MDRQEWLAGQFDEHRPHLRAVAYRMLGSLSEAEDAVQDAWLRLG
                     RTDTDEVENLRAWLTTVVARVALNMLRGRRTRREQPLDVHLPDPIVDPADGTDPEHEA
                     LLADSVGLALLVVLETLTPAERLAFVLHDMFAVPFEEIAPIVERSPEATRQLASRARR
                     RVRGAAPVPDPDLPAQWEVVEAFLAAAREGDFDALVAVLDPDVVLRSDGGPTGPTHHV
                     RGAEAVAGQALLWSRVDLTMRRALVNGAAGMVAFRRGQPFSLAAITVRNGRIAELDFL
                     ADPERIAQLDLTVLGD"
     gene            2708..>2904
                     /locus_tag="VFO88_05950"
     CDS             2708..>2904
                     /locus_tag="VFO88_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545100.1"
                     /translation="MGEARPRAASYPERIRWAVETLAVEPADRVLEIGGGPGVAASLV
                     CERLDRGRLLVIDRSATAIERT"
BASE COUNT          395 a         1072 c         1040 g          397 t
ORIGIN      
        1 cgaccgccgg ggcgcgatga gttcggctcc ctccgacggt ccgagctccg aacccgcagg
       61 aaaggagatc cgatgggcaa gctggtcgtg accgagttcg tgtccctcga cggcgtcatg
      121 caggcgcccg gcggcgagga cttcaagtat ccgggctgga gcttcgcctt cgaccgcggc
      181 gacgacggga accagttcaa gctcgacgag accctcgaga ccgaggcgct gctgctcgga
      241 cgagtgacgt acgagagctt cgccggcgcg tggccggaac gcgagggcga gttcgccgac
      301 aagttcaaca cgatgccgaa gtacgtcgtc tcgtcgacgc tgaaggatcc ggagtggaac
      361 aacaccaccg tcgtcgacgg cgacgtggtc ggcgaggcgg cgaagctgaa ggagcggacc
      421 gacgggatcc tccagacgcc gggcagcctc cggctcgtgc agacgctgct cgagcacgac
      481 ctcgtcgacg agctccatct gatggtgttc ccggtcatcc tcggaacggg ccggcgcctg
      541 ttcggcgaga cgaccgagaa gacggactgg cggctgaccg aggcgaggcc ggtcgggccg
      601 gacggcgtgc tcgtcctgat ctacgagcgg gccaggtagg caacgtggcg ggccggatgg
      661 agtgcacgcg cgagcgcccc tagccgggcg actgacgcgt ccgcgcggcc gggtgaggct
      721 cgggccggtc ggccgtgcgc atcgccgagt aggcggcggc gaaccccctg cgccagctcg
      781 ggtaacgcgg cgcccagccg agctcgcgcc gcgcctttgc gttcgacgcg ccccgcgcct
      841 ccgtgcccag cacgaccgct gcctcacccg cgaccagccg cgcgagccac gccgggatgc
      901 ggcgaggcgg cttcgcgtcg agggcctccg cgagcacggg cagccattcc cgcaccggcg
      961 ccggctcgtc gtccaccacg ttgtagatcg ccgggccgtc gtgctcgagc gcgagcacgg
     1021 tggccgcggc ggcgtcgtcg aggtggatcc aggagaacat cccgcccccc tcgccgacga
     1081 tcgggaactg ccgcttgcgc accggctcga tcaggccgtc gttggcggcg ccgtagaagc
     1141 cgccgtagcg aaggacgatc ccgccgaagc cggtcaccgc ctgctcgaga taggtcatcg
     1201 cgccggtgct ctcctcggtc ttccgcggcg ggtcgggatc gagcggatcg tcctcggtct
     1261 tgaccgggcc gccctcccgc gcgtgccggt aggaggcgaa gctctgcgcg acgaaccgag
     1321 gcaccccggc ctcgcgcgcg gccgcgagca gcgcgtcggt ccccttcgtg cgcagcgcgt
     1381 tcgtcggccc gaacaccttg tcgaagtcgc ggccgagctt cacgtccgcg agcgcggtcg
     1441 cctggtgcac gatcgcctcg ggctcgctct cgagcacggc acggcgcacc gcctccgcgt
     1501 cgagcaggtc gagccgcacc ggcttcgcgc cgagcgtcca cacgtgcgcc gccctggacg
     1561 gcgagcggtg ggtgccgatc acctcgtggc ctgcgtcgat cagctgcgga acgagccggc
     1621 tgccgagcgc gccgctcgct cctgcgacga aaacacgcat gtgtcctccc ttgtgagtcc
     1681 tccggtcaca tcccgcggat ctggcgggtc tacctccatg acacgcggcg gaggaggaat
     1741 gtgacgtgga caggcaggag tggctcgccg ggcagttcga cgaacaccga ccgcatctgc
     1801 gcgcggtcgc ctatcgcatg ctcggctcgc tcagcgaggc cgaagacgcc gtccaggacg
     1861 cctggctacg gctcggccgc acggacacgg acgaggtcga gaacctccgg gcctggctga
     1921 cgacggtcgt cgcgcgcgtc gcgctcaaca tgctccgcgg gcgcaggacg cggcgcgagc
     1981 agccgctcga cgtccacctc cccgatccga tcgtcgaccc cgccgacggg accgaccccg
     2041 agcacgaggc gctgctcgcc gactcggtcg gcctcgcgct gctcgtggtg ctcgagacgc
     2101 tgacgccggc ggagcggctt gcgttcgtcc tccacgacat gttcgccgtc cccttcgagg
     2161 agatcgcccc gatcgtcgag cgctcccccg aggcgacgcg ccagctcgcg agccgcgcgc
     2221 gccggcgcgt ccgcggcgcc gctcccgtcc cggaccccga cctgcccgcc cagtgggagg
     2281 tcgtcgaggc gttcctcgcc gccgcccggg agggcgactt cgacgcgctc gtcgccgtgc
     2341 tcgaccccga cgtcgtgctg cgatccgacg gcggtccgac cggcccgacg caccacgttc
     2401 ggggtgccga ggccgtcgcc gggcaggcgc tcctctggtc gcgcgtcgac ctgacgatgc
     2461 gccgcgcgct cgtgaacggc gccgccggga tggtcgcgtt ccgccgcggg cagccgttct
     2521 cgctcgccgc gatcaccgtg cgaaacggca ggatcgccga gctcgacttc ctcgccgacc
     2581 ccgagcggat cgcgcaactc gacctcacgg tgctcggcga ctagcgcgtc ccctcaggcg
     2641 agccggaagg tctgcgcgac ccggcggtca tcggccggcg gcactcctgt accttgcgct
     2701 gtcagccatg ggggaggcgc gaccacgggc ggcctcgtat ccggaacgga ttcgctgggc
     2761 ggtcgagacg ctcgccgtcg agccggcgga ccgcgtgctc gagatcggcg gcgggcccgg
     2821 ggtcgccgcc tcgctcgtgt gcgagcgcct cgaccgcggc cgcctgctcg tgatcgaccg
     2881 ttcggcgacg gcgatcgagc ggac
//
LOCUS       DASTFC010000199         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_646607,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000199 DASTFC010000000
VERSION     DASTFC010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646607"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VFO88_05955"
     CDS             complement(<1..1543)
                     /locus_tag="VFO88_05955"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET9545101.1"
                     /translation="MAQNLGSFEKVLRVGEGRRLKRLAQQADYVATLEPDFEGLSDAE
                     LAGKTAEFRQRLDNGEDVNDLVFEAYAAVREAAKRTLGMRHFDVQVMGGIVLHEGDIA
                     EMKTGEGKTLVATMPLYLNALPGDNVHLVTVNDYLAKRDTEWMGPVYEALGLRAAFIR
                     NLMPFAERKEAYSADITYGTNSEFGFDYLRDNMATSLDNTVQRHHRFAIVDEVDSILI
                     DEARTPLIISGEPETAGKTYYDFARVAKTLRGAAVIPGAKGEEAYGDADYTYDEKFKT
                     VSPFESAIEKVEQALGIENLYDPRNAQLVNHLIQALKAESLYHRDDDYVVMDGEVKIV
                     DEFTGRIMEGRRWSEGLHQAVEAKERVPIKEEHVTLATITLQNYFRLYEKLSGMTGTA
                     KTEEKEFKEIYDLSVVEIPTNVPMARDDKNDFIYKTRPAKYKAVMEDIKERHESGQPV
                     LVGTISVEVSEHLSQLLTRAGITHNVLNAKQHEREAEIIKDAGQPGAVTIATNMAGRG
                     VDIKLG"
     gene            complement(1564..2118)
                     /locus_tag="VFO88_05960"
     CDS             complement(1564..2118)
                     /locus_tag="VFO88_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545102.1"
                     /translation="MEHPRSRLLEVLGQLPSGVTGIPRLKEWDAVALVEVPELEASPL
                     RELELDALPDGGLDARADEAVPREALERLAAELDTRLARPYEALAVRRDGRLWAIGAN
                     AVRLGEPLELGDLPAGTVELVRAPGGDTTLSVDGEDVDPGDEALYAAAAAELARVGSE
                     RFESFVARADRGAGGRWHVTVDPL"
     gene            complement(2125..2676)
                     /gene="raiA"
                     /locus_tag="VFO88_05965"
     CDS             complement(2125..2676)
                     /gene="raiA"
                     /locus_tag="VFO88_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET9545103.1"
                     /translation="MQLQMKAKNVELTPALKAYAEKKLGRLGRVLSDSTRVELELAAE
                     RNPSIGESQIAEATVWTKGPVLRARESARDMKAAIDLLVDKLDRQAERLHENRRRRGA
                     RAGSASAAAPAPAPALDEEGRHVIVKTKQFTVKPMDAEEAIVQLELIGHDFFVFQNDE
                     TNAVNVLYRRRDGNYGLIEPQVT"
     gene            complement(2731..>3127)
                     /locus_tag="VFO88_05970"
     CDS             complement(2731..>3127)
                     /locus_tag="VFO88_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545104.1"
                     /translation="EAAALVAEAVARPDVEALAYVPGDGERLLWRGHNTAEELARALG
                     ARWELPVVDALARTGRAPRQRGRGRVERRANVRGAFRPRGEAPRRVALVDDVYTTGAT
                     ANAAASAVRKGGARAVDVVTFARTLRAAA"
BASE COUNT          381 a         1120 c         1088 g          538 t
ORIGIN      
        1 cgccgagctt gatgtcgacg ccgcggcccg ccatgttcgt cgcgatcgtg accgcgcccg
       61 gctggccggc gtccttgatg atctcggcct cgcgctcgtg ctgcttcgcg ttcaggacgt
      121 tgtgcgtgat ccccgcacgc gtgaggagct gcgagaggtg ctcggagacc tcgaccgaga
      181 tcgtgccgac gagcaccggc tggccggact cgtggcgctc cttgatgtcc tccatcacgg
      241 ccttgtactt cgcgggccgg gtcttgtaga tgaagtcgtt cttgtcgtcg cgcgccatcg
      301 ggacgttcgt cgggatctcg acgacggaga ggtcgtagat ctccttgaac tccttctcct
      361 cggtcttcgc cgtcccggtc atgccggaga gcttctcgta gaggcggaag tagttctgga
      421 gcgtgatcgt cgcaagggtg acgtgctcct ccttgatggg gacgcgctcc ttcgcctcga
      481 ccgcctggtg gaggccctcg ctccagcggc gtccctccat gatccggccg gtgaactcgt
      541 cgacgatctt cacctcgccg tccatgacga cgtagtcgtc gtcgcggtgg tagagcgact
      601 cggccttgag cgcctggatc aggtggttca cgagctgggc gttccgcggg tcgtagaggt
      661 tctcgatccc gagcgcctgc tcgaccttct cgatcgccga ctcgaacggg gagacggtct
      721 tgaacttctc gtcgtacgtg tagtccgcgt cgccgtaggc ctcctcgccc ttcgcgccgg
      781 gaatgaccgc ggcgcccctg agggtcttcg cgacgcgcgc gaagtcgtag tacgtcttgc
      841 ccgcggtctc cggctcgccc gagatgatga gcggcgtccg cgcctcgtcg atcaggatcg
      901 aatcgacctc gtccacgatc gcgaaccgat ggtgccgctg cacggtgttg tcgagcgacg
      961 tcgccatgtt gtcgcgcagg tagtcgaagc cgaactcgct gttcgtgccg tacgtgatgt
     1021 cggccgagta cgcctccttg cgctccgcga acggcatgag gttgcggatg aacgcggcgc
     1081 gcaggccgag cgcctcgtac accgggccca tccactcggt gtcgcgcttc gccaggtagt
     1141 cgttcaccgt cacgaggtgg acgttgtcgc cggggagcgc gttcaggtag agcggcatcg
     1201 tcgcgacgag cgtctttccc tcgccggtct tcatctcggc gatgtcgccc tcgtggagga
     1261 cgatcccgcc catcacctgc acgtcgaagt ggcgcatccc gagcgtccgc ttcgccgcct
     1321 cgcggacggc ggcgtacgcc tcgaagacga ggtcgttcac gtcctcgccg ttgtcgagcc
     1381 gctgccggaa ctcggccgtc ttgccggcga gctcggcatc cgagagcccc tcgaagtcgg
     1441 gctcgagcgt cgccacgtag tcggcctgct gggcgaggcg cttgaggcgc ctgccctcgc
     1501 cgacgcggag gaccttctcg aaggagccga ggttctgagc catgaatctt ccagggtagc
     1561 tggttacagg ggatcgaccg tgacgtgcca gcggcccccg gctcccctat cggcacgggc
     1621 gacgaaggac tcgaatcgct ccgagccgac gcgcgcgagc tcggccgccg cggcggcgta
     1681 cagggcctcg tcgcccgggt cgacgtcctc gccgtcgacg ctcagcgtcg tgtccccgcc
     1741 cggcgcccgg acgagctcga ccgtcccggc cgggaggtcg ccgagctcga gcggctcgcc
     1801 gagccgcacg gcgttcgccc cgatcgccca caggcgcccg tcccgccgta cggcgagcgc
     1861 ctcgtacggc cgtgcgagcc gggtgtcgag ctccgccgcg agccgttcga gcgcctcgcg
     1921 cggcacggcc tcgtcggccc gcgcgtcgag cccgccgtcg ggaagcgcgt cgagctcgag
     1981 ctctcgcagc ggcgaggcct cgagctcggg cacctcgacg agcgctaccg cgtcccactc
     2041 cttcagacgc gggatcccgg tgacgccgga cggaagctgc ccgaggacct cgaggagccg
     2101 ggagcgcggg tgctccatcg cgggctaggt cacctgaggc tcgatgagcc cgtagttccc
     2161 gtcgcggcgg cggtagagga cgttgacggc gttcgtctcg tcgttctgga agacgaagaa
     2221 gtcgtgtccg atcagctcga gctggacgat cgcctcctcg gcgtccatcg gcttcaccgt
     2281 gaactgcttc gtcttgacga tcacgtgacg tccctcctcg tccagggccg gagccggtgc
     2341 cggcgcggcg gcgctcgccg agcccgcgcg cgcgccgcgg cggcggcggt tttcgtggag
     2401 ccgctccgcc tgccggtcga gcttgtccac cagcaggtcg atcgcggcct tcatgtcgcg
     2461 ggcgctctcg cgcgcgcgga gcacggggcc cttcgtccac acggtcgcct cggcgatctg
     2521 gctctcgccg atcgacgggt ttcgctcggc ggccagctcg agctcgacgc gggtcgagtc
     2581 gctcaggacg cgccccaggc gcccgagctt cttctccgcg taggccttga gcgccggggt
     2641 cagttcgacg ttcttcgcct tcatctggag ctgcaatccg ggcctcctcg tcgcggtcgc
     2701 cgaacctggt cggagcgtac ccgatgccgg tcacgcggcc gctctgagcg tccgggcgaa
     2761 cgtgacgacg tcgacggcgc gcgccccacc cttgcggacc gcggacgcgg cggcgttcgc
     2821 ggtcgcgccg gtcgtgtaca cgtcgtccac gagcgcgacg cggcgcggcg cctctccccg
     2881 gggacggaag gcgccgcgga cgttggcgcg ccgctccacc ctcccccgcc cacgctggcg
     2941 cggcgcccgg ccggtgcgcg caagggcgtc cacgacgggg agctcccagc gggcgccgag
     3001 cgcgcgtgcg agctcctcgg ccgtgttgtg gccgcgccag aggagccgct cgccgtcgcc
     3061 cgggacgtag gcgagcgcct cgacgtccgg ccgtgcgacc gcctcggcga cgagcgcggc
     3121 cgcctcg
//
LOCUS       DASTFC010000200         7442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_349920,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000200 DASTFC010000000
VERSION     DASTFC010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349920"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFO88_05975"
     CDS             complement(<1..692)
                     /locus_tag="VFO88_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9545105.1"
                     /translation="MATTEAKTYRNYIGGEWVEAASGESFETRNPATGETLGVFPLSS
                     TEDMDRAVEAAKAAFEGWRLVPAPKRGELLFRVAQMFAERKDELTEQMVREMGKVRAE
                     AAGDVQEAIDMTFYMAAEGRRMFGHTTPSELPDKFMMSTRNPVGVVGAITPWNFPIAI
                     PSWKIAPALVCGNTVVFKPAEDTPLLAGLFVEIFEEAGLPPGVLNIVHGAGETVGEHL
                     VKHPDVPLISFTG"
     gene            843..1286
                     /locus_tag="VFO88_05980"
     CDS             843..1286
                     /locus_tag="VFO88_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545106.1"
                     /translation="MTRRIATLTAVAAASAALAASALAAGAGGVTGPAFYVDGQLYRT
                     VATPTDLPANAPAKSFDVIYELFGLQPNVAEAAPGDPDYNGGRWLVHGIEFDDYEAAV
                     DAFDANGSGDFDSAEEIEAALAAGAATDLGVVKRFVCPAIKLPQG"
     gene            1371..2051
                     /locus_tag="VFO88_05985"
     CDS             1371..2051
                     /locus_tag="VFO88_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9545107.1"
                     /translation="MSTVLVVDDEPIVREVVVSYLRREGYRTLEAGDGHEARRLLEQG
                     DPGLVVLDLMLPGIDGLQLCRWIRSRSDVPVIMLTARGEETDRIVGLELGADDYVTKP
                     FSPRELAARVRAVLRRADGTGEGAERRLEFGDVTIEASTREATKDGSPLALTAREFDL
                     LWFLASHPRRVFSREQLMDRVWGYAAAVDTGTVTVHVRRLRAKIERDPSRPEHLQTVW
                     GVGYRLVP"
     gene            2048..3112
                     /locus_tag="VFO88_05990"
     CDS             2048..3112
                     /locus_tag="VFO88_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9545108.1"
                     /translation="MIELALLVGAGTLAAGVVAALGLRALPTVRLQVAALACLSVALP
                     LAAVFLSGWAMFHMGDDVKILAVASAAATAATGAGLLIARSIANRLDRVREAAGALAQ
                     GDLGSRVPEGGPAELNELAASFNRMAEALERLFDARRELVAWASHDLRTPLASMQAMV
                     EALEDGLGEPERYLPALREQVRTLSTLVDDLFELSRIDSEALVLDLRDTRLDELVASC
                     VRALEPEADARRIRLSTRIGAPATARCAPEKVERVLFNLLTNALRHTPSDGAVAVSLD
                     AANGTVRIAVEDTGDGLTPDAAQRMFERFWRGDRARSGGGAGLGLAIARGLVEAQGGR
                     IWAESRAGGGARVCFTLPGV"
     gene            complement(3109..3342)
                     /locus_tag="VFO88_05995"
     CDS             complement(3109..3342)
                     /locus_tag="VFO88_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545109.1"
                     /translation="MIYCVVPPELKDELFDRLVEHYKDNPNVTVIVDRRTGPDRRSAA
                     GGLLGGKRQVRDRRRGRIPGTFPPTDAPEDQPA"
     gene            complement(3389..4015)
                     /locus_tag="VFO88_06000"
     CDS             complement(3389..4015)
                     /locus_tag="VFO88_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9545110.1"
                     /translation="MHAEIRDLAPGLWIWRVEHPDWSPDAGWEPPVTSTCVESGGEVA
                     VLDALLPPGGGTEAVRERLDARPPTLAVVLKPDHVRSVDEVVRRYGARAYGPSLFWPH
                     DVPETALEPIEPGSRLPGGLEALYDGRGRNETPLWLPEQLTIVFADALTAPGGELRVW
                     ATPWHEERALPALRALLELPFERVIVAHGEPVHDRAAYERALDLEPFS"
     gene            complement(4018..4632)
                     /locus_tag="VFO88_06005"
     CDS             complement(4018..4632)
                     /locus_tag="VFO88_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545111.1"
                     /translation="MTRRQEAFRTCAVCNRTLLQGERAARYSPGGDDHWVDVCALCLD
                     QANEYGWLKEGTPRTPIVPEAPRRRRRSWNLGSLFETRREIPEPVLAEPVLRRLSPDE
                     QLLVEAAELFNKSQHSRTIAGVAKSLGEARVSMIPLSGTNAEIVITIAWEISWYQYRV
                     LFDSAQPVRLEDRGQDVDELRDRFKHWNASFDAQHRLRPDIPRV"
     gene            complement(4664..6718)
                     /gene="fusA"
                     /locus_tag="VFO88_06010"
     CDS             complement(4664..6718)
                     /gene="fusA"
                     /locus_tag="VFO88_06010"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9545112.1"
                     /translation="MAAYESGKIRNIAVVGHRGTGKTSLVEALLFQAGATNRLGSVEQ
                     GSTVSDWEEEEQRRQMSLSAGLAHLEWQGRKVNLIDTPGDQGFQADTVAALRVVEGAV
                     MVVNAVAGVEVQTSRIWQRCEELELSRILFVNMLDRERADFFAALEAIRGQLSDRCVA
                     VEIPIGHEHDLKGVVDLLHMKAYLDPNGEREGGQAEIPDEIAEQAQAYRTQLIDAVVE
                     TDEAVMERYLEGEEISGDELAAALKNAVSRDELFPVACGVATKNLGTAALLDLIVEGI
                     PSPAKKPLKVEAPESGDAAFVFKTVADPFAGRITIFRVLAGEVSGDANLVNPRTHGKE
                     RLGQLLALQGKDHAQADGFAAGDIGAVAKLKETTTGDLLLSQDAPVEPPSLDFPQPVM
                     SFAITPKAKGDEEKVATALRRLAEEDPTLDIHRDDQTGELLLGGLSQMHVEVAVERMK
                     RRFGAEVELHQPRVPYLETIRKESRAQGRYKKQTGGRGQYGDCVIVLEPLPEHDGYEF
                     VDQIVGGVIPQGFRPAVDKGIQEAMQRGELAGAPVQGVRVRLVDGSYHNVDSSEMAFK
                     IAGSMAFKQAYESASPVLLEPIMTLEATVPDDAVGAVNGDLNSRRGRLLGMEPAAGMT
                     TIKAEVPMAEILTYSQSLTSLTGGRGDYSMSFLRYEEVPSHVAQKIVDEAKREREEAK
                     AS"
     gene            complement(6983..>7442)
                     /locus_tag="VFO88_06015"
     CDS             complement(6983..>7442)
                     /locus_tag="VFO88_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545113.1"
                     /translation="RDIERSIAQLEAKLGRAPNDEEISTHLGISEEEFQDSLLEISRS
                     SIAALDELWTGSGGGDPVALIDTIEDPEAPEPQAAMAQTELREALGEAIARLPEREKL
                     VVTLYYYEELTLREIGEVLGVTESRVSQLHTKAILRLKARLGGSVPHAPA"
BASE COUNT          930 a         2717 c         2698 g         1097 t
ORIGIN      
        1 cccgtgaacg agatgagcgg gacgtcgggg tgcttcacga gatgctcgcc gaccgtctcc
       61 cccgcgccgt ggacgatgtt caggacgccc ggcgggaggc ccgcctcctc gaagatctcg
      121 acgaagagcc cggcgaggag cggcgtgtcc tcggccggct tgaagacgac ggtgttgccg
      181 cagacgagcg cgggcgcgat cttccagctc gggatggcga tcgggaagtt ccacggcgtg
      241 atcgcgccga cgacgccgac cgggttccgc gtcgacatca tgaacttgtc cgggagctcc
      301 gacggcgtcg tgtggccgaa catccgccgg ccctccgccg ccatgtagaa cgtcatgtcg
      361 atcgcttcct gaacgtcgcc cgcggcctcg gcgcgcacct tccccatctc gcgcaccatc
      421 tgctcggtca gctcgtcctt gcgctcggcg aacatctggg cgacgcggaa gaggagctcg
      481 ccgcgcttcg gcgccggcac gagccgccac ccctcgaacg cggccttcgc ggcctcgacc
      541 gcgcggtcca tgtcctccgt cgaggacagc gggaagacgc cgagcgtctc gcccgtcgcg
      601 gggttcctcg tctcgaagct ctcgccgctc gcggcctcga cccactctcc gccgatgtag
      661 ttccggtacg tcttcgcttc cgtcgtcgcc accctgctcc ccttcgctcg ctgacgcggc
      721 cgattctacg cgcgggcggc cgcgtccccg gcggccacgt tcaccgcggt ttaagacctg
      781 cgcaagggcg gcgcaagatc gggccgcgac gcttcagagg tcaagcgacg aggaggttct
      841 ccatgacccg acgcatcgcg acgctcaccg ccgtcgccgc cgcgtccgcc gcgctcgccg
      901 cctccgcgct cgcggccggc gcgggcggcg tgaccggccc ggcgttctac gtggacggcc
      961 agctctaccg gacggtcgcc acgccgaccg acctgcccgc gaacgcgccc gcgaagagct
     1021 tcgacgtgat ctacgagctc ttcgggctcc agccgaacgt cgccgaggcc gcgcccggcg
     1081 accccgacta caacggcggg cgctggctcg tccacgggat cgagttcgac gactacgagg
     1141 ccgccgtcga cgcgttcgac gcgaacggca gcggcgactt cgacagcgcg gaggagatcg
     1201 aggcggcgct cgcggccggc gcggcgaccg acctcggtgt cgtcaagcgc ttcgtctgcc
     1261 cggcgatcaa gctgccccag gggtagcagg atcggcgaat cgccgggccc gggacgcgcg
     1321 ggcccggcga tttcatccct ccgtaagctc cgacgcgcca cactgtgggc atgagcaccg
     1381 tcctcgtcgt cgacgacgag ccgatcgtgc gcgaggtcgt cgtctcgtac ctccgccggg
     1441 agggctaccg gacgctcgag gccggcgacg gccacgaggc ccgccgcctg ctcgagcagg
     1501 gcgacccggg cctcgtcgtc ctcgacctca tgctgccggg gatcgacggc ctccagctct
     1561 gccgctggat ccggagccgc tccgacgtcc cggtgatcat gctcaccgcc cgcggcgagg
     1621 agaccgaccg catcgtcggg ctcgagctcg gcgcagacga ctacgtgacg aagccgttct
     1681 cgccccgcga gctcgcggca cgcgtccggg ccgtgctccg gcgggcggac ggcacgggcg
     1741 agggggccga gcggcggctc gagttcggcg acgtgacgat cgaggcctcg acgcgcgagg
     1801 cgacgaagga cggaagcccg ctcgccctca ccgcgcgcga gttcgacctc ctctggttcc
     1861 tcgcctcgca cccgcggcgg gtcttctcgc gggagcagct catggaccgc gtctggggct
     1921 acgcggccgc ggtcgacacg gggacggtga ccgtgcacgt ccgcaggctg cgcgcgaaga
     1981 tcgagcgcga cccgtcgcgc ccggagcacc tgcagaccgt ctggggcgtc ggctaccggc
     2041 tcgtcccgtg atcgagctcg ccctcctcgt cggagccggg acgctcgcgg cgggcgtcgt
     2101 cgccgcgctc ggcctccgtg cgctgccgac cgtgcgtctc caggtcgcgg cgctcgcgtg
     2161 cctctccgtc gcgctgccgc ttgcggccgt cttcctgtcc ggctgggcga tgttccacat
     2221 gggcgacgac gtgaagattc tcgcggtcgc gtcggcggcg gcaacggcgg cgaccggggc
     2281 cgggctcctc atcgcccgct cgatcgcgaa ccggctcgac cgggtgcggg aggcggcggg
     2341 cgcgctcgcc cagggcgacc tcgggtcgcg ggtccccgag ggcggcccgg ccgagctgaa
     2401 cgagctcgcg gcctcgttca accggatggc ggaggcgctc gagcggctct tcgacgcccg
     2461 gcgggagctc gtggcctggg cgagccacga cctccgcacg ccgctcgcgt cgatgcaggc
     2521 gatggtcgag gcgctcgagg acggcctcgg ggagcccgag cgctacctcc ccgcgctccg
     2581 cgagcaggtg cggacgctct cgacgctcgt cgacgacctc ttcgagctct cgcggatcga
     2641 ctcggaggcc ctcgtgctcg acctccgcga cacgcggctc gacgagctcg tcgcgtcgtg
     2701 cgtccgcgcg ctcgagccgg aggccgacgc ccgccggatc cggctctcga cgcggatcgg
     2761 cgcgccggcg acggcccgct gcgcgcccga gaaggtcgag cgcgttctct tcaacctcct
     2821 gacgaacgct ctccgccaca cgccgtcgga cggtgcggtc gcggtctcgc tcgacgcggc
     2881 gaacggaacc gtgcggatcg cggtcgagga cacgggcgac ggcctcacgc ccgacgcggc
     2941 gcagcggatg ttcgagcgct tctggcgcgg cgaccgcgcg cgctccggcg gcggcgcagg
     3001 gctcgggctc gcgatcgcgc gcggcctcgt ggaggcccag ggcggccgga tctgggccga
     3061 gagccgcgcc ggcggcggcg cgcgcgtctg cttcaccctt ccgggcgtct aggccggctg
     3121 gtcctcgggg gcgtcggtcg gcgggaacgt ccccgggatg cgaccccggc gccggtcgcg
     3181 cacctggcgc tttccgccga gcagcccgcc cgcggccgag cgccggtcgg gcccggtgcg
     3241 ccggtcgacg atcacggtca cgttcgggtt gtccttgtag tgctcgacga ggcggtcgaa
     3301 gagctcgtcc ttgagctccg gagggacgac gcagtagatc accgtgggcg catcgtaaac
     3361 ggcgccgcgc ccggcgccgc accgctcgtc aggagaacgg ctcgaggtcg agggcgcgct
     3421 cgtacgccgc gcggtcgtgg acgggctcgc cgtgggcgac gatcacgcgc tcgaacggga
     3481 gctcgagaag cgcgcggagc gccggcagcg cccgctcctc gtgccacggc gtcgcccaga
     3541 cgcgcagctc gccgccgggc gcggtgagcg cgtcggcgaa gacgatcgtc agctgctcgg
     3601 gaagccagag cggcgtctcg ttccgcccgc gcccgtcgta gagcgcctcg agcccgccgg
     3661 ggaggcggct gcccggctcg atcggctcga gcgcggtctc cgggacgtcg tgcggccaga
     3721 agaggctcgg cccgtacgcg cgtgcgccgt agcggcggac gacctcgtcg acgctccgca
     3781 cgtggtcggg cttgaggacg acggcgagcg tgggcgggcg cgcgtcgagg cgctcccgaa
     3841 cggcctccgt gccgcccccc ggcggaagga gcgcgtcgag caccgcgacc tcgccgccgg
     3901 actcgacgca cgtggaggtg acgggcggct cccacccggc gtcggggctc cagtctggat
     3961 gctcgacgcg ccagatccag agaccgggcg cgagatctct gatctccgcg tgcacggcta
     4021 gacccggggg atgtccggcc gcagccggtg ctgggcgtcg aagctcgcgt tccagtgctt
     4081 gaagcggtcg cggagctcgt ccacgtcctg cccccggtcc tcgagccgca ccggctgcgc
     4141 ggagtcgaag aggacgcggt actggtacca ggagatctcc caggcgatcg tgatcacgat
     4201 ctccgcgttc gtgcccgaga gcgggatcat gctgacgcgc gcctcgccga ggctcttcgc
     4261 gacaccggcg atcgtgcggc tgtgctggct cttgttgaag agctccgcgg cctcgacgag
     4321 gagctgctcg tccggcgaga ggcggcggag caccggctcg gcgaggaccg gctcggggat
     4381 ctcccggcgg gtctcgaaga gcgagccgag gttccacgag cggcggcgcc tgcgcggcgc
     4441 ctccgggacg atcggcgtgc gcggcgtccc ctccttcagc cacccgtact cgttcgcctg
     4501 gtcgaggcag agcgcgcaga cgtcgaccca gtggtcgtcg ccgccgggcg agtagcgcgc
     4561 cgcccgctcc ccctggagga gcgtgcgatt gcagacggcg caggtcctga acgcctcctg
     4621 ccgccgcgtc atcgtcacgg cctcggggcg ggacgacgca tggctacgag gccttcgctt
     4681 cctctcgctc gcgcttggcc tcgtcgacga tcttctgcgc cacgtgggag ggcacctcct
     4741 cgtagcgcag gaagctcatc gagtagtcgc cgcggccgcc ggtgagggac gtgagcgact
     4801 gcgagtacgt gaggatctcg gccatcggca cctcggcctt gatggtcgtc atgccggcgg
     4861 cgggctccat cccgagcagg cgtccgcgcc gcgagttgag gtcgccgttc accgcgccga
     4921 ccgcgtcgtc ggggacggtc gcctcgagcg tcatgatcgg ctcgaggaga accgggctcg
     4981 cgctctcgta ggcctgcttg aaggccatcg agccggcgat cttgaacgcc atctcggagg
     5041 agtcgacatt gtggtacgag ccgtccacga gccgcacgcg caccccctgc accggcgcgc
     5101 ccgcgagctc gccccgctgc atcgcctcct ggatcccctt gtcgaccgcc ggccggaacc
     5161 cctgggggat cacgccgccg acgatctggt cgacgaactc gtacccgtcg tgctcgggga
     5221 gcggctcgag gacgatcacg cagtcgccgt actggccgcg gccgcccgtc tgcttcttgt
     5281 agcgcccctg cgcccgggac tccttgcgga tcgtctcgag gtacgggacg cgcggctggt
     5341 ggagctcgac ctcggcgccg aagcggcgct tcatccgctc caccgcgacc tcgacgtgca
     5401 tctgggagag gccgccgagg aggagctcgc cggtctggtc gtcgcggtgg atgtcgaggg
     5461 tcgggtcctc ctcggcgagc cggcggagcg ccgtcgcgac cttctcctcg tcacccttcg
     5521 ccttcggcgt gatcgcgaag ctcatgaccg gctgggggaa gtcgagcgac ggcggctcga
     5581 ctggcgcgtc ctgggaaagg agcaggtcgc cggtcgtcgt ctccttgagc ttcgccacgg
     5641 cgccgatgtc gcccgccgcg aagccgtccg cctgcgcatg gtcctttccc tgaagcgcga
     5701 ggagctggcc gagccgctcc ttgccgtgcg tgcgcgggtt gacgaggttc gcgtcgccgg
     5761 agacctcccc ggcgagcacg cggaagatcg tgatccggcc ggcgaacggg tcggcgaccg
     5821 tcttgaagac gaacgcggcg tcgccggact cgggcgcctc gaccttgagc ggcttcttcg
     5881 cgggagacgg gatgccctcg acgatgaggt cgaggagcgc ggccgtgccg aggttcttcg
     5941 tcgcgacgcc gcacgcgacc gggaagagct cgtcgcggct gacggcgttc ttgagcgcgg
     6001 cggcgagctc gtcgccggag atctcctcgc cctcgaggta gcgctccatg accgcctcgt
     6061 ccgtctcgac gacggcgtcg atcagctgcg tgcggtacgc ctgcgcctgc tcggcgatct
     6121 cgtccgggat ctcggcctgg cccccctccc gctccccgtt cgggtcgagg tacgccttca
     6181 tgtgcaggag gtcgacgacg cccttcaggt cgtgctcgtg gccgatcggg atctcgacgg
     6241 cgacgcagcg gtcggagagc tggccgcgga tcgcctcgag cgcggcgaag aagtccgcgc
     6301 gctcgcggtc gagcatgttg acgaagagga tgcgcgagag ctcgagctcc tcgcagcgct
     6361 gccagatgcg gctcgtctgc acctcgacgc cggcgaccgc gttcacgacc atgaccgccc
     6421 cctcgacgac gcggagcgcg gcgaccgtgt ccgcctgaaa gccctggtcg ccgggcgtgt
     6481 cgatcaggtt caccttgcgg ccctgccact cgaggtgtgc gaggccggcc gagagcgaca
     6541 tctggcgccg ctgctcctcc tcctcccagt cggagacggt cgagccctgc tcgacggagc
     6601 cgagccggtt cgtggcgccc gcctggaaga ggagcgcctc gacgagggat gtcttccccg
     6661 tcccccggtg ccccacgacg gcgatgttgc ggatcttccc cgattcgtac gcggccatcc
     6721 gggcgcgagt ctaccgaggc gttgccggcg ggcgaggaga gtgcgcgccg cgctttccgg
     6781 ttagccgcgc gggacctcgg gccacccccg ggggtgggtc gtcagacggc ggtgcccagc
     6841 gtaccgccga ccggcgcgcg cgatcgtgcc gatcgtgtgt cgactgcgtg ccgattcgtc
     6901 cccggcctgc tgaggattga cgtgaacgag ccggggcgcg ccgggcggcg cctcgagcgc
     6961 ggcgtctctc tcgcggaagc ctctaggcgg gcgcgtgcgg cacggagccg ccgaggcgcg
     7021 ccttgaggcg caggatcgcc ttcgtgtgga gctgcgagac ccgcgactcg gtcacgccga
     7081 gcacctcgcc gatctcgcgc agcgtcagct cctcgtagta gtagagcgtg acgacgagct
     7141 tctcccgctc gggcaggcgg gcgatcgcct cgccgagcgc ctcccgcagc tcggtctgcg
     7201 ccattgccgc ctggggctcg ggcgcctcgg gatcctcgat cgtgtcgatg agcgcgacgg
     7261 ggtcgccgcc gcccgagccc gtccagagct cgtcgagcgc cgcgatcgag gaccgcgaga
     7321 tctcgagcag cgagtcctgg aactcctcct cgctgatccc gaggtgcgtc gagatctcct
     7381 cgtcgttcgg cgcccggccg agcttcgcct cgagctgggc gatcgagcgc tcgatgtcgc
     7441 gc
//
LOCUS       DASTFC010000201         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1223724,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000201 DASTFC010000000
VERSION     DASTFC010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223724"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..351)
                     /locus_tag="VFO88_06020"
     CDS             complement(121..351)
                     /locus_tag="VFO88_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545114.1"
                     /translation="MPDDRLHVELAFEGGQMLRAVVREDGVRALEAGLARGDGGTLEL
                     EAEDGTYVIALRSVVYLKRFSRETRIGFGAAG"
     gene            complement(344..745)
                     /gene="mce"
                     /locus_tag="VFO88_06025"
     CDS             complement(344..745)
                     /gene="mce"
                     /locus_tag="VFO88_06025"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET9545115.1"
                     /translation="MQLLGVHHVALAVEDLDAAVATYRTLFDAEIELEERNEEQGVDA
                     VYLRVGDGRVELVSPLDDDTPVGRFLAKRGEGMHHVAFEVDDVDGAVGELASRGAQMI
                     DDEPRPGLAGKRVAFVHPESLSGVLAEIVGA"
     gene            complement(785..2314)
                     /locus_tag="VFO88_06030"
     CDS             complement(785..2314)
                     /locus_tag="VFO88_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545116.1"
                     /translation="MDAVVEHIGRLPEYALTWLPLVFLGLIVYLIWRTLQFMPRVKPT
                     TVEPDSKSSVRWDDIAGVDEAAKELQEVVDFLQHPKRFAQLGARVPKGILLYGPPGTG
                     KTLLAKAVANASGASFYSQSASAFVEMFAGLGAARIRKLFETARNNAPAIVFIDELDA
                     VGMRRSGTSHNREHDQTLNQLLVELDGFRSPDQVVLIGASNRLEDLDPALLRPGRFDR
                     QILVSPPDLAGREAILEVHTRGKPLADDVDLNVVARHTAGLAGADLANICNEAAIFAG
                     RAERGEVTQGDFDAAIERIVAGLQQSRVVTEKEKRILAYHEAGHALMSHLVGEPMPVQ
                     KVTIVSRGQALGYTLNTPQEDRYLHTKDEFLDLLKVMLAGRAAEQIVFGAVTNGAAND
                     LERVTELARRMVFEFGMSDEVASRTMRADNYALSEHTKRVRDEEQARLTDHAYSEAMR
                     LLAKHRASLDRLSALLLQKETLHRPELEELFAEIERESNSSEAVGTPRVVAMPSDPSA
                     R"
     gene            complement(2345..>2617)
                     /locus_tag="VFO88_06035"
     CDS             complement(2345..>2617)
                     /locus_tag="VFO88_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545117.1"
                     /translation="GVNPRLVEKVRAALDAEGFRDVKIVVSGGFSAERIREFEERGIP
                     VDSYGVGSSLIRGDNDFTADIVLTEGKPSAKVGRRYIPNPRLEPVL"
BASE COUNT          354 a          925 c          950 g          388 t
ORIGIN      
        1 agtgtcctcg ccaaccccgt tgtcctctct ggacgcctcc cccggacggg gtagtgcctc
       61 cactcgcacc taagcagaga gctgccgaaa agcaacccgg cgcgcgacgc gccgcggggg
      121 ctacccggcg gcgccgaaac cgatgcgcgt ctcgcgcgag aaccgcttga ggtagacgac
      181 cgagcggagg gcgatgacgt acgtgccgtc ctccgcctcg agctcgagcg tgccgccgtc
      241 gccgcgcgcg agcccggcct cgagcgccct gacgccgtcc tcgcgcacga cggcgcgcag
      301 catctggccg ccctcgaacg cgagctcgac gtggaggcgg tcgtcaggca ccgacgatct
      361 ccgcgaggac gcccgagagc gactcggggt ggacgaacgc gacgcgcttc ccggcgaggc
      421 cgggccgcgg ctcgtcgtcg atcatctgcg cgccgcggct cgcgagctct ccgaccgcgc
      481 cgtccacgtc gtcgacctcg aacgcgacgt ggtgcatgcc ctcgccgcgc ttggcgagga
      541 agcgcccgac cggtgtgtcg tcgtcgagcg gggagacgag ctcgaccctc ccgtcgccca
      601 cgcggaggta cacggcgtcg acgccctgct cctcgttccg ctcctcgagc tcgatctcgg
      661 cgtcgaagag cgtgcggtac gtcgcgaccg cggcatcgag gtcctcgacc gcgagggcga
      721 cgtggtggac gccgaggagc tgcacggcgc cgatcgtacc gtccgcggtc ggccgcgggg
      781 ccggtcagcg cgccgagggg tcgctcggca tcgcgacgac gcgcggggtg ccgaccgcct
      841 cggaggagtt cgactcgcgc tcgatctccg cgaagagctc ctcgagctcc gggcggtgga
      901 gcgtctcctt ctggagcagc agcgcggaga gccggtcgag cgacgcgcgg tgcttcgcga
      961 ggagccgcat cgcctcggag tacgcgtggt cggtgagccg ggcctgctcc tcgtcgcgga
     1021 cgcgcttcgt gtgctcggag agcgcgtagt tgtccgcgcg catcgtgcgg gaggcgacct
     1081 cgtcgctcat cccgaactcg aacaccatcc ggcgcgcgag ctcggtcacc cgctcgaggt
     1141 cgttcgcggc gccgttcgtg accgcgccga acacgatctg ctcggccgcg cgccccgcga
     1201 gcatcacctt gaggaggtcg aggaactcgt ccttcgtgtg gaggtagcgg tcctcctgcg
     1261 gcgtgttcag cgtgtagccg agcgcctggc cgcgggagac gatcgtcacc ttctgcaccg
     1321 gcatcggctc gccgacgagg tgcgacatga gggcgtggcc cgcctcgtgg taggcgagga
     1381 tccgcttctc cttctccgtg acgacgcggc tctgctggag gccggccacg atgcgttcga
     1441 tcgcggcgtc gaagtcgccc tgcgtcacct cgccgcgctc ggcgcgcccg gcgaagatcg
     1501 ccgcctcgtt gcagatgttc gcgaggtcgg cgcccgcgag gcccgccgtg tggcgggcga
     1561 cgacgttcag gtcgacgtcg tcggcgagcg gcttgccgcg cgtgtgtacc tcgaggattg
     1621 cctcgcgccc ggcgagatcg ggcggcgaga cgaggatctg ccggtcgaag cgccccgggc
     1681 ggaggagcgc cgggtcgagg tcctcgagcc ggttcgacgc gccgatcagg acgacctggt
     1741 ccgggctcct gaacccgtcg agctcgacga ggagctggtt cagggtctgg tcgtgctcgc
     1801 ggttgtggct cgtgccggag cggcgcatcc cgaccgcgtc gagctcgtcg atgaagacga
     1861 tcgccggcgc gttgttccgc gccgtctcga agagcttgcg gatccgcgcc gcgccgagcc
     1921 cggcgaacat ctcgacgaag gcggacgcgc tctgcgagta gaagctcgcg ccggaggcgt
     1981 tcgcgacggc cttcgcgagg agcgtcttcc cggtcccggg cgggccgtag aggaggatcc
     2041 ccttcggcac ccgcgcgccg agctgggcga agcgcttcgg gtgctggagg aagtcgacga
     2101 cctcctgcag ctccttcgcc gcctcgtcca cgcccgcgat gtcgtcccag cgcacggagg
     2161 acttcgagtc cggctcgacc gtcgtcggct tcacgcgcgg catgaactgc agcgtgcgcc
     2221 agatcaggta gacgatcagg ccgaggaaga cgagcggaag ccacgtcagg gcgtactcgg
     2281 gcagcctccc gatgtgctcg acgaccgcgt ccacggagtt cccatcggcg gcgtcgggcg
     2341 cggactaaag gaccggctcg aggcgcgggt tcgggatgta gcgccgcccg accttcgcgg
     2401 acggcttccc ctcggtgagg acgatgtccg cggtgaagtc gttgtcgccg cggatgagcg
     2461 aggagccgac gccgtacgag tcgaccggga tgccgcgctc ctcgaactcg cggatccgct
     2521 cggccgagaa gccgcccgag acgacgatct tcacgtcgcg gaagccctcc gcgtcgagcg
     2581 ccgcgcgcac cttctccacg agcctggggt tcacgcc
//
LOCUS       DASTFC010000202         4384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_234546,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000202 DASTFC010000000
VERSION     DASTFC010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234546"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VFO88_06040"
     CDS             <1..1163
                     /locus_tag="VFO88_06040"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET9545118.1"
                     /translation="FVGPQWGEVRGFALPPARDGMPIDPGPAPRLDDGRTSDEFKRTA
                     LEIVRYSSQLDPSDGVRMEIGPAGRGGNALGTNDGEGHEVNPATGRPYEPNVVARGDF
                     ARALAEYWADGPDSETPPGHWNTIANEVSDRPELEHRIGGSGPVVDRLEWDVKLYLAL
                     NGAVHDAAVAAWGAKAYYDSARPISMIRYLGSKGQSSDPDGPSYHPDGLPLERGLVEV
                     VTRASSRPGERHAALAGHVGEIAIRAWAGFPGDPVAEASGVRWIRAVEWVPYQLPTFV
                     TPAFAGYVSGHSTFSRAAAEVMTAFTGSSYFPGGIHELPVPAGWLKIERGPSTDFTFQ
                     WATYFDAADDAGRSRLYMGIHISPDDFAGRRLGSQCGKDAWELARRYYEGAV"
     gene            1189..2193
                     /locus_tag="VFO88_06045"
     CDS             1189..2193
                     /locus_tag="VFO88_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9545119.1"
                     /translation="MKTSLGIWAFGPMATRFVPGGYQPEHADESTVDRVRRAVDGLGD
                     LIDDYEFHYPQELSEENLDDVREALGDHGIYCIATGLHLDPLFGRGGLTSPDPETREE
                     ARRRTRAAADFAGSLGAHFIIWPGIEGYNYPFQTPYGESWAWLVDGVAEAAERCAEHG
                     VKLFLEHKNSEPAMKIFMRNIGMTLHVVHKLRERGITNVQVNMDWQHLIMNGENLAEY
                     AAMLDAEGLLGHQHANSGWGVFDDDNMVGATAFMETLELALELRRAGYGASGERLGFD
                     LYPYTEDQVEAVRRSVLQWRFIDSVAARIDDAALREAQSRKDAVRAYELVYAALGADA
                     "
     gene            2190..2582
                     /locus_tag="VFO88_06050"
     CDS             2190..2582
                     /locus_tag="VFO88_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET9545120.1"
                     /translation="MRLEQARAMVDAVVSAAPGPVSVFVADDHGELVAAATMDGAAPD
                     TRLNAQRKAYTAARSDVPATSLLAEKVRDDPAELASFDPAFSFFKGGVAAFDGDRRVG
                     AIGVSGLPGEEDERLAREAIERAGLTAP"
     gene            2579..4015
                     /gene="xylB"
                     /locus_tag="VFO88_06055"
     CDS             2579..4015
                     /gene="xylB"
                     /locus_tag="VFO88_06055"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET9545121.1"
                     /translation="MTALVGLDVGTTGVKALAVSPDGDVLARAESSYGFETPRPGWAE
                     QDPESWWRGTEEALAALGVGDVAGIGLSGQMHGLVLLDGDERVLRPAILWNDQRTAAE
                     CVEIEELLGGRRRLVEATGNRALPGFTAPKLLWVRRHEPETYERAAHVLLPKDYVRLR
                     LTGERAIDAADASGTLLFDVGARRWSEEVLAALELPAAWLPPALESPEVSGRTAAGVP
                     VAAGAGDQAAGALGVGVDRPGPLSVVLGTSGVVFAALPGYAADPEGRVHVFCHAVPDG
                     WHAMGVMLSAAGSLQWLRDTIAPGEPFGALVAEAERWEPGAEGLVFLPYLAGERTPHV
                     DPDARGAFVGLSLRHDRGALVRAVLEGVAYALRDSLELLRALGVEPASARVSGGGARS
                     DLWLRIVASVLDLPLERTAAEEGAAYGAALLGGVAAGVFADVHEAVAATVRVTGTVEP
                     EPAWRETYEAGYARFRELYPSLHTEEAT"
     gene            4012..>4384
                     /locus_tag="VFO88_06060"
     CDS             4012..>4384
                     /locus_tag="VFO88_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9545122.1"
                     /translation="MTSLEGKVALVTGASRGIGAAVAHALAAQGARLGLASRSGDDLG
                     IDGAVAQPCDVRRPDELESLARATAERHGGIDVLVANAGVGAYGPFLELPPEHLEEMI
                     DVNVKGTLYAVRATLPYLLESD"
BASE COUNT          572 a         1574 c         1681 g          557 t
ORIGIN      
        1 cgttcgtcgg cccccagtgg ggcgaggtcc gaggcttcgc gctcccgccc gcccgggacg
       61 ggatgccgat cgacccgggc cccgctccga ggctcgatga cgggaggacg tcggacgagt
      121 tcaagcggac ggcactcgag atcgtccgct actcctcgca gctcgacccg tccgacggcg
      181 tccggatgga gatcggcccc gccggccgcg gcggaaacgc gctcgggacg aacgacgggg
      241 aggggcacga ggtcaatccg gccacgggac gcccgtacga gccgaacgtc gtcgcgcgcg
      301 gcgacttcgc ccgggcgctc gcggagtact gggccgacgg gcccgactcc gagacgcctc
      361 ccggccactg gaacacgatc gcgaacgagg tctccgaccg gcccgagctc gagcaccgga
      421 tcggcggctc ggggcccgtc gtcgaccggc tcgagtggga cgtgaagctc tacctcgccc
      481 tgaacggggc cgtccacgac gccgccgtcg ccgcctgggg cgcgaaggcg tactacgact
      541 cggcgcgtcc gatctcgatg atccgctacc tcggctcgaa ggggcagtcg agcgacccgg
      601 acggcccgtc gtaccacccg gacgggcttc cgctcgagcg ggggctcgtc gaggtcgtga
      661 cgcgcgcctc gagccggccc ggggagcggc acgcggcgct cgcgggccac gtcggcgaga
      721 tcgcgatccg ggcgtgggcc ggcttccccg gcgacccggt cgcggaggcg agcggcgtcc
      781 gctggattcg cgcggtcgag tgggtcccgt accagctccc gacgttcgtc acgcccgcgt
      841 tcgcgggcta cgtctccggc cactcgacgt tctcgcgtgc ggccgccgag gtgatgacgg
      901 cgttcaccgg cagctcctac ttcccgggcg gcatccatga gctgccggtg ccggcggggt
      961 ggctgaagat cgagcggggg ccgagcacgg acttcacgtt ccagtgggcg acgtacttcg
     1021 acgccgccga cgacgccggc cggtcgcgcc tctacatggg gatccacatc tcgccggacg
     1081 acttcgcggg ccgccggctc ggctcgcagt gcggcaagga cgcgtgggag ctcgcgcgac
     1141 gctactacga gggggcggtg tgaccatcga tcacaacagg agggacgcat gaagacgagt
     1201 ctcgggatct gggccttcgg gccgatggcg acgcggttcg tgccgggggg ctaccagccg
     1261 gagcatgcgg acgagtcgac cgtggaccgc gtccgccgcg ccgtggacgg cctcggcgac
     1321 ctgatcgacg actacgagtt ccactacccg caggagctct cggaggagaa cctcgacgac
     1381 gtgcgggagg cgctcggcga ccacggcatc tactgcatcg cgacggggct ccacctcgac
     1441 ccgctcttcg ggcgcggcgg gctcacctcg cccgacccgg agacgcgcga ggaggcgcgc
     1501 cgccgcacgc gcgccgcggc cgacttcgcc ggctcgctcg gcgcgcactt catcatctgg
     1561 ccggggatcg agggctacaa ctacccgttc cagacgccct acggcgagag ctgggcgtgg
     1621 ctcgtggacg gcgtcgcgga ggcggccgag cgctgcgcgg agcacggcgt gaagctcttc
     1681 ctcgagcaca agaactccga gcccgcgatg aagatcttca tgcggaacat cgggatgacg
     1741 ctgcacgtgg tccacaagct ccgcgagcgc gggatcacga acgtccaggt gaacatggac
     1801 tggcagcacc tgatcatgaa cggcgagaac ctcgccgagt acgcggccat gctcgatgcc
     1861 gaggggctgc tcggccacca gcacgcgaac tcgggctggg gcgtgttcga cgacgacaac
     1921 atggtcggcg cgacggcgtt catggagacg ctcgagctgg cgctcgagct gcgtcgcgcg
     1981 ggatacggcg cgagcggcga gcggctcggc ttcgacctct acccgtacac ggaggaccag
     2041 gtcgaggcgg tgcggcggag cgtcctccag tggcgcttca tcgactcggt cgcggcgcgg
     2101 atcgacgacg ccgcgctccg cgaggcgcag tcgagaaagg acgcggtgcg cgcgtacgag
     2161 ctcgtctacg cggcgctcgg ggcggacgcg tgaggctcga gcaggcgcgt gcgatggtgg
     2221 acgcggtcgt ctcggcggcg cccggcccgg tctcggtgtt cgtggcggac gaccacgggg
     2281 agctcgtcgc ggcggccacg atggacggcg ccgcgccgga cacgcgcctg aacgcgcagc
     2341 ggaaggcgta cacggccgcc cggagcgacg ttcccgcgac gagcctgctt gccgagaagg
     2401 tgcgcgacga cccggccgag ctcgcgagct tcgatcccgc gttcagcttc ttcaagggcg
     2461 gcgtcgccgc gttcgacggc gaccggcgcg tgggcgcgat cggcgtcagc ggcctgcccg
     2521 gcgaggagga cgagcggctc gcccgcgagg cgatcgagcg cgccggcctc acggcgccgt
     2581 gacggcgctc gtcgggctgg acgtcggcac gaccggcgtg aaggcgctcg ccgtctcgcc
     2641 cgacggggac gtgctcgcac gcgcggagtc ctcgtacggc ttcgagacgc cgcgccccgg
     2701 ctgggccgag caggacccgg agtcgtggtg gcgcggcacc gaggaggcgc tcgcggcgct
     2761 cggggtgggc gacgtcgcgg gcatcgggct ctcgggccag atgcacgggc tcgtcctcct
     2821 cgacggcgac gagcgggtgc tccggccggc gatcctctgg aacgaccagc gcaccgcggc
     2881 ggaatgcgtg gagatcgagg agctgctcgg gggccgccgg cggctcgtcg aggcgaccgg
     2941 gaaccgcgcc ctgcccgggt tcaccgcgcc gaagctcctc tgggtgcgcc gccacgagcc
     3001 ggaaacgtac gagcgggccg cgcacgtgct gctcccgaag gactacgtgc ggctgcggct
     3061 gaccggcgag cgcgcgatcg acgcggcgga cgcctccggg acgctcctct tcgacgtcgg
     3121 cgcgcggcgc tggagcgagg aggtgctcgc ggcgctcgag ctcccggcgg cgtggctgcc
     3181 gcccgcgctc gagtcgccgg aggtctcggg ccgcacggcc gccggcgtcc cggtcgccgc
     3241 cggcgcgggc gaccaggcgg ccggcgcgct cggggtcggc gtcgaccggc cgggaccgct
     3301 ctccgtcgtg ctcgggacgt cgggcgtcgt cttcgcggcg ctgcccggct acgcggccga
     3361 ccccgaaggg cgcgtgcacg tcttctgcca cgcggtgccc gacggctggc acgcgatggg
     3421 cgtcatgctc tccgcggccg ggtcgctcca gtggctgcgc gacacgatcg cgcccgggga
     3481 gccgttcggc gcgctcgtcg cggaggccga gcgctgggag ccgggcgccg aggggctcgt
     3541 gttcctcccg tatctcgccg gcgagcggac gccgcacgtc gacccggacg cgcgcggcgc
     3601 cttcgtcggg ctctcgctcc gccacgaccg gggcgcgctc gtccgggcag tgctcgaggg
     3661 cgtcgcatac gcgctccgcg actcgctcga gctcctgcgc gcgctcggcg tcgagcccgc
     3721 ctcggcgcgc gtctcgggcg gcggggcgcg cagcgacctc tggctccgga tcgtcgcgtc
     3781 cgtgctcgac ctgccgctcg agcggacggc ggccgaggag ggcgccgcgt acggggcggc
     3841 gctgctcggc ggcgtcgccg cgggcgtctt cgccgacgtc cacgaggcgg tcgccgcgac
     3901 cgtccgcgtg accgggaccg tcgagcccga gcctgcctgg cgcgagacgt acgaagccgg
     3961 gtacgcccgc tttcgagagc tctatccatc cctgcacacg gaggaggcca catgacatcg
     4021 ctggaaggaa aggtcgccct cgtcacgggc gcgagccgcg ggatcggcgc ggccgtcgcc
     4081 cacgccctgg ccgcgcaggg ggcgcgcctc gggctcgcct cgcgaagcgg cgacgacctc
     4141 gggatcgacg gcgccgtcgc gcagccgtgc gacgtccgca ggccggacga gctcgagtcg
     4201 ctcgcacggg ccacggcgga gaggcacggc ggcatcgacg tcctcgtcgc gaacgcgggc
     4261 gtcggcgcct acgggccgtt cctcgagctc ccgcccgagc acctcgagga gatgatcgac
     4321 gtcaacgtga agggcacgct gtacgcggtg cgcgcgacgc ttccgtacct cctcgagagc
     4381 gacg
//
LOCUS       DASTFC010000203         6683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_861132,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000203 DASTFC010000000
VERSION     DASTFC010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861132"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VFO88_06065"
     CDS             complement(<1..1338)
                     /locus_tag="VFO88_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9545123.1"
                     /translation="MPLRPGGRERAAVPLSDGTRLAARLWLPEELPAAVLLEALPYRM
                     DDLTASYASEYERLCAEGGFAVARVDLRGTGSSDGIATDEYPPQELDDLAEVVAWLAA
                     QEWSTGRVGMFGTSYSGFNSLQLACERPPALGAVAAIYATDDRYTDDVHYTGGALRGL
                     DLLDYVLYMAAMNVLPPVPAVYGEGWRDAWRERVEAAEPWLLRWLEEQADGPYWRHGS
                     LRPGYERIACPTMLVGGWADGYRNNTFRTFERLEAPRRLLVGPWSHMSPATSLPGPHI
                     DLVPELSRWFARWLRDERNGVDEEPPIAVFIRRSTQPAPDLAEVRGEWRAEAGWPLER
                     ARELDLRPEPPDGLDELRVEGDVGTAAWISCAARLPWGQPGDQREDDARSLAYEWEPL
                     AEELEILGHARLRLRIETAAPVAFLSAKLCDVFPDGTSALVTRGLLNLAHRESS"
     gene            1540..1668
                     /locus_tag="VFO88_06070"
     CDS             1540..1668
                     /locus_tag="VFO88_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545124.1"
                     /translation="MEDKRSEPDRADEERETEREEGLGEGPEGWDDLEETADEARS"
     gene            complement(1696..1974)
                     /locus_tag="VFO88_06075"
     CDS             complement(1696..1974)
                     /locus_tag="VFO88_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545125.1"
                     /translation="MTLAPSSILDGPARGERAMLRSVALLPLRALRWVVLGLLGWLLA
                     LLVAAAIGAAVGFAAGLLAAPWYGWESLAIGPVVGAMLAAHIAPGSCR"
     gene            complement(2098..3066)
                     /locus_tag="VFO88_06080"
     CDS             complement(2098..3066)
                     /locus_tag="VFO88_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545126.1"
                     /translation="MRIHRTFVTAAAALAAAAFAGAAQAASPGQLLRQHQPVVVFHPQ
                     EEFRPTTVESFVRDSDLEAATSPTEWALVDAAPTVGTLPAASPPIWRLNQRDCFAGAP
                     LGDLACYTAAAADEPDPVVYGRVARDGGRTVLQYWLFYYDNVYRYPFLPLGAIWQSHE
                     GDWEVVNVVLGPGRKPVAVGISRHCSGETRPWSDTPRMGRHPVVHVALGSHANYFEPG
                     VHEFDRACLPPEVIGFFAQAGLPLPADVAASGPSEGIGRTGAPYAVRRISDASPSWVA
                     FPGFWGELEYFNAPPPIGTVPFGTGPRGPAFHEVWDAPLATLAEWS"
     gene            complement(3053..3541)
                     /locus_tag="VFO88_06085"
     CDS             complement(3053..3541)
                     /locus_tag="VFO88_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545127.1"
                     /translation="MTPRRRPRWASTGFFAAVVLAFFLPFGTVSCSGSKTPVTGIQLA
                     TWTVADEAYADAIESEASLLAALALVATLVGLGLSAWGVTRGPGWCAGVALAAFAGLG
                     WKAYYWGVETEAGFSIAWLLLGVVWVLHVVGVLQRRQDKRRMAAVAPEKITTERSQGA
                     YP"
     gene            3683..4039
                     /locus_tag="VFO88_06090"
     CDS             3683..4039
                     /locus_tag="VFO88_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9545128.1"
                     /translation="MARTVLVVDDHAAFRAAARRLLELDGLRVVGEAEDGATALRLVP
                     VLAPDIVLLDVGLPDLSGFEVADRLADGPAQVVFVSSRAPGDLGRRVRSTGARGFIAK
                     DDLSGEEVLRLCMPIG"
     gene            4036..5784
                     /locus_tag="VFO88_06095"
     CDS             4036..5784
                     /locus_tag="VFO88_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9545129.1"
                     /translation="MTVVTAGIVAAGLGLGILAYQVQVDELRQFSSPENATAIVAVAW
                     AFLVAGLVAWRRRPGNLLGPLMMLAAFALLLRQFRYSHDDWTFTVFFLVSEVSYALVA
                     HSVLAYPGGRVTDRLERAFLAVGYTATLVFPLAILLAYDGTRPLRFFDPRPRESAIAV
                     VGDAELAVAIQKAFVVFVWGVLATVFIALLIRRLVRATPVGRRLLAPLLLAAVAVALR
                     AVFEGVFTFVERPSEILYTYLFWWQITGFIALPLALLLGLLRARLARAGVGDLVLELE
                     DAPPRRIREALARTLRDPTLEVAFWLPEESRFVDADGRPVTLPEDDPRRGVTRLEHDG
                     DPIAALVHDPALAQEPRLVAAAGAAARLALENARLQAETRAQLATVKESRARIVEAAD
                     DERRRIERDLHDGAQQRLVALALQLRSAQRELHPDGDVERILEESVRELQAAVEELRE
                     LARGVHPAILTEEGLAPALESLAHRTPIEVDLDVAEERLPAAVEATAYFVVCEALANV
                     VKHARASRASVSANRLDGLLRVEVADDGVGGARAEPGSGLSGLADRVEALGGRLLVVS
                     DEGAGTRVVGEIPCGS"
     gene            5772..6416
                     /locus_tag="VFO88_06100"
     CDS             5772..6416
                     /locus_tag="VFO88_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9545130.1"
                     /translation="MRVVIADDSVLLREGLARVLRDGGFDVVGQAADGAALEASVART
                     RPDVAVVDVRMPPTQTDEGTRAAKAIRERHPEVGLLLLSQVVEPRHALDLFSEHPERF
                     GYLLKDRVLEIDDFLDAVRRVARGGTVVDPEVVAQLLGRRRAPDPLEDLTPREREVLA
                     LMAEGRSNRAISQKLFLSPKTIETHVNSIFLKLRLVQEPDDHRRVLAVLAFLRS"
     gene            complement(6527..>6683)
                     /locus_tag="VFO88_06105"
     CDS             complement(6527..>6683)
                     /locus_tag="VFO88_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9545131.1"
                     /translation="EEVAEFGQDNPLGRPAQPAELAPAYVFLASDEARFVVGEVIGVT
                     GGLLLPS"
BASE COUNT          903 a         2457 c         2494 g          829 t
ORIGIN      
        1 ggaggattcg cggtgcgcga ggttcaggag cccgcgcgtg acgagggcgg acgtcccgtc
       61 cgggaagacg tcgcagagct tcgccgagag gaacgcgacc ggcgcggcgg tctcgatgcg
      121 cagacgcagg cgcgcgtggc cgaggatctc gagctcctcg gcgagcggct cccactcgta
      181 cgcgagcgag cgcgcgtcgt cctcgcgctg gtcgcccggc tggccccacg ggagccgcgc
      241 cgcgcacgag atccacgcgg ccgttccgac gtcgccctcg acgcggagct cgtccaggcc
      301 gtcgggcggc tcgggccgca ggtcgagctc gcgggcgcgc tcgagcggcc agcccgcctc
      361 ggcacgccat tcgccgcgaa cctcggcgag atccggcgcg ggctgggtcg agcggcggat
      421 gaagacggcg atcggcggct cctcgtccac gccgttccgc tcgtcgcgga gccagcgagc
      481 gaaccagcgg gagagctccg gcacgaggtc gatgtgcggg ccgggcagcg acgtggccgg
      541 cgacatgtgg ctccacgggc cgacgagcag ccgcctcggc gcctccaggc gctcgaaggt
      601 gcggaacgtg ttgttgcggt agccgtccgc ccagcctccg acgagcatcg tcggacaggc
      661 gatccgctcg tagcccggcc gaagcgagcc gtggcgccaa tacgggccgt cggcctgctc
      721 ctcgagccag cggaggagcc acggctccgc ggcctcgacc cgctcgcgcc acgcgtcgcg
      781 ccaaccctcg ccgtacaccg ccgggaccgg cgggaggacg ttcatcgcgg ccatgtagag
      841 gacgtagtcg aggaggtcga ggccgcgcag cgcgccaccc gtgtagtgga cgtcgtccgt
      901 gtagcggtcg tccgtcgcgt agatcgcggc gaccgcgccg agcgcgggcg ggcgctcgca
      961 ggcgagctgg agcgagttga agccggagta ggacgtgccg aacatcccga cgcggccggt
     1021 cgaccactcc tgcgccgcga gccacgcgac cacctcggcc aggtcgtcga gctcctgcgg
     1081 cgggtactcg tcggtcgcga tcccgtccga ggagccggtt ccgcgcaggt cgacgcgcgc
     1141 gaccgcgaag ccaccctcgg cgcagagccg ctcgtactcg gacgcgtacg aggccgtgag
     1201 gtcgtccatc cgatacggga gcgcctcgag gagcacggcg gcgggcagct cctcgggcag
     1261 ccagagccgg gccgcgagcc gcgtcccgtc ggagagcggg acggcggcgc gctcacgccc
     1321 tcccgggcga agaggcatcc cggctacacg cgcgcccgtg atgagggaag accccctcca
     1381 cccactagtg aaagcgtatc gaggagaagc gcgcgtgtct gtgccgcttc gtgccggaac
     1441 cgtgccgagg gcgtgccgat ggcgcccggg acgccgaccg ggccggtcgt gtgttcgacc
     1501 cggctcgggt gggaacccgg cgccggagag gaggtgcgaa tggaggacaa gcggagcgag
     1561 cccgaccggg ccgacgagga gcgggagacg gagcgcgagg aagggctcgg cgagggcccc
     1621 gaggggtggg acgacctcga ggagacggcg gacgaagcgc gctcgtagcg cgcgaggggc
     1681 cggcccgggc cggcgctacc ggcaggaccc aggtgcgatg tgagccgcga gcatcgcgcc
     1741 gaccaccggg ccgatcgcga gcgactccca gccgtaccac ggcgccgcga gcaggccagc
     1801 ggcgaacccg acggcggctc cgatcgccgc tgcgacgagc agcgcgagga gccacccgag
     1861 gagcccgagg acgacccagc gaagcgcacg gagcggcagg agcgcgacgg agcgaagcat
     1921 cgcccgctcg ccgcgggccg ggccgtccag gatggaggag ggagcgagcg tcacgcttcg
     1981 agcctgcgac tgcccgcccg cgccctggaa cagggcaggt ccgctaccgc cctcggggtc
     2041 gccccggaat tcgccgggcg ccgcgcggcc ccacccgcgc ggcgcccttc ccccgcgcta
     2101 cgaccactcg gcgagcgtcg cgagcggcgc gtcccagacc tcgtggaagg ccgggccgcg
     2161 gggacccgtc ccgaacggca cggttccgat cggaggcggc gcgttgaagt actcgagctc
     2221 gccccagaag ccgggaaacg cgacccagga cggagaggcg tccgagatgc gccgcaccgc
     2281 atacggcgcc ccggtccggc cgatcccctc ggacgggccg gacgcggcca cgtcggccgg
     2341 cagcggcagc cccgcctgcg cgaagaagcc gatcacctcg ggcgggaggc acgcccggtc
     2401 gaactcgtgc acccccggct cgaagtagtt ggcgtgcgag ccgagcgcga cgtgcacgac
     2461 gggatgacgc cccatccgcg gcgtgtccga ccaggggcgc gtctcgccgg agcagtgccg
     2521 gctgatgccc acggcgacag gcttgcggcc ggggccgagc accacgttca cgacctccca
     2581 gtcgccctcg tgggactgcc agatcgcgcc gagcgggagg aacggatagc ggtacacgtt
     2641 gtcgtagtag aagagccagt actggagcac cgtgcggccc ccgtcgcgcg ccacgcgccc
     2701 gtagacgacg ggatccggct cgtccgccgc cgccgccgtg tagcaggcga ggtcgccgag
     2761 cggggctccc gcgaagcagt cgcgctggtt cagccgccag atcggcgggc tcgcggccgg
     2821 cagcgtcccg accgtcggcg cggcgtcgac gagcgcccac tccgtcgggc tcgtcgcggc
     2881 ctcgaggtcg gagtcgcgga cgaacgactc gaccgtggtc ggccggaact cctcctgcgg
     2941 gtggaacacg accaccggct ggtgctggcg gagcagctgc cccggcgacg cggcctgggc
     3001 cgcgccggcg aaggccgcgg ccgcgagcgc ggcggccgcc gtcacgaagg tcctatggat
     3061 acgcaccctg gctcctttcg gtcgtgatct tctcgggcgc gaccgccgcc atccgccgct
     3121 tgtcctgccg gcgctgaagc acgcccacca cgtggagcac ccacacgacg ccgagcagca
     3181 accatgcgat cgagaacccg gcctcggtct cgacgcccca gtagtacgcc ttccacccga
     3241 gcccggcgaa cgccgcgagc gccacacccg cgcaccagcc gggccctctc gtgacacccc
     3301 aggcggaaag cccgagaccg acgagcgtcg cgaccagcgc gagcgcggcg aggagcgagg
     3361 cctcgctctc gatcgcatcc gcgtacgcct cgtccgcgac ggtccacgtg gccagctgga
     3421 tgccggtgac cggggtctta gagcccgagc aggaaacggt tccgaacggt aggaagaacg
     3481 cgagaacgac cgccgcaaag aagccggtgg aggcccagcg aggtcggcgt cgaggcgtca
     3541 tgccgcgacc gtacggcggc cggcgcggcg gcggaacccg gcccgcccgg gactcgtctc
     3601 ggggtagacc ctgattcgcc gtgggggcga aatcggggtt ttcccggtag aaaccgggac
     3661 ccgggtgtgt gacgatcaac acgtggctcg gacggttctc gtcgtcgacg accacgcggc
     3721 gtttcgggcg gcggccaggc ggttgctcga gctcgacgga ctgcgcgtcg tcggagaggc
     3781 ggaggacggg gcgactgcgc tccgcctggt tcccgtgctc gcgcccgaca tcgtcctgct
     3841 cgacgtgggc ctgcccgacc tgagcggctt cgaggtcgcc gaccgcctcg cggacgggcc
     3901 ggcacaggtc gtgttcgtct cgagccgggc ccccggcgac ctcggtcggc gtgtgcgttc
     3961 gaccggcgcg cgcgggttca tcgcgaagga cgacctgagc ggggaggagg tgctccgcct
     4021 ctgcatgccc atcggatgac ggtggtcacg gcggggatcg tcgcggccgg gctcgggctc
     4081 ggcatcctcg cgtaccaggt gcaggtggac gagctgcgcc agttcagctc gcccgagaac
     4141 gccaccgcga tcgtcgccgt cgcctgggcg ttcctcgtcg ccggcctcgt ggcctggcgg
     4201 cggcgcccgg ggaacctcct gggcccgctc atgatgctgg ccgcgttcgc gctcctcctg
     4261 cgccagttcc gctacagcca cgacgactgg acgttcacgg tcttcttcct cgtcagcgag
     4321 gtgagctacg cgctcgtcgc ccactcggtg ctcgcctatc ccgggggccg ggtgaccgac
     4381 cggctcgagc gggccttcct cgcggtcggc tacacggcga cgctcgtctt cccgctcgcg
     4441 atcctcctcg cctacgacgg gacgaggccg ctgcgcttct tcgatccgcg tccgagggag
     4501 agcgcgatcg ccgtcgtcgg agacgccgag ctcgcggtcg cgatccagaa ggcgttcgtc
     4561 gtcttcgtct ggggcgtgct cgcgaccgtc ttcatcgcgc tcctgatccg caggctcgtg
     4621 cgggcgacgc cggtggggcg gcggctgctc gctccgctcc tgctcgcggc ggtcgccgtc
     4681 gcgctgcggg cggtgttcga gggcgtcttc acgttcgtcg agcgcccctc ggagatcctc
     4741 tacacgtatc tcttctggtg gcagatcacg gggttcatcg cgcttccgct cgctctgctc
     4801 ctcgggctcc tgcgcgcccg gctcgcccgg gccggggtcg gcgatctcgt gctcgagctg
     4861 gaggatgcgc cgccgcgccg catccgcgag gcgctcgcgc gcacgctgcg cgacccgacg
     4921 ctcgaggtcg ccttctggct cccggaggag agtcgattcg tcgacgccga cggccgcccg
     4981 gtcaccctgc ccgaggacga cccgcgccgc ggcgtcacgc ggctcgagca cgacggcgac
     5041 ccgatcgcgg cgctcgtcca cgacccggcg ctcgcgcagg agccgcgcct ggtcgccgcc
     5101 gccggcgcgg ccgcccggct cgcgctcgag aacgcgcgcc tgcaggccga gacgcgggcg
     5161 cagctcgcga ccgtgaagga gtcgcgcgcc cggatcgtcg aggctgccga cgacgagcgg
     5221 cggcggatcg agcgcgacct gcacgacggg gcgcagcagc ggctcgtcgc gctcgcgctc
     5281 cagctgcgga gcgcccagcg cgagctccac cccgacggcg acgtggagcg gatcctcgag
     5341 gagtccgtgc gcgagctcca ggcggcggtc gaggagctgc gcgagctcgc gcgcggcgtg
     5401 cacccggcga tcctgaccga ggagggcctg gcgcccgcgc tcgagtcgct cgcgcaccgg
     5461 acgccgatcg aggtcgacct cgacgtggcg gaggagcggc tgcccgcggc ggtcgaggcg
     5521 accgcgtact tcgtcgtctg cgaggcgctc gcgaacgtcg tcaagcacgc gcgcgcgagc
     5581 cgcgcgtcgg tgagcgcgaa ccggctcgac gggctcctgc gggtcgaggt cgccgacgac
     5641 ggggtcggag gcgcgcgcgc ggagcccggc tcgggcctga gcggcctcgc cgaccgggtc
     5701 gaggcgctcg gcgggcgcct gctcgtcgtc agcgacgaag gggccgggac gcgcgtcgtc
     5761 ggggagatcc catgcgggtc gtgatcgcgg acgactcggt cctcctccgc gagggcctcg
     5821 cccgcgtcct tcgcgacggc ggcttcgacg tcgtcggcca ggcggccgac ggggccgcgc
     5881 tggaggcgag cgttgcccgc acgcggccgg acgtcgccgt cgtcgacgtg cggatgccgc
     5941 cgacgcagac ggacgagggg acacgcgccg cgaaggcgat ccgcgagcgc caccccgagg
     6001 tcggcctgct cctgctctcg caggtcgtcg agccccggca tgcgctcgat ctcttcagcg
     6061 agcaccccga gcggttcggc tacctcctga aggatcgagt cctcgagatc gacgacttcc
     6121 tcgacgccgt ccggcgggtc gcgcgcggcg gcaccgtcgt cgacccggag gtcgtcgcgc
     6181 agctcctcgg ccggcgccgc gcgcccgacc cgctcgagga cctgaccccg cgtgagcgcg
     6241 aggtcctcgc gctcatggcc gagggccgct cgaaccgcgc gatctcgcag aagctcttcc
     6301 tcagcccgaa gacgatcgag acgcacgtga actcgatctt cctgaagctc cggctcgtcc
     6361 aggagccgga cgaccaccgg cgcgtcctcg ccgtcctcgc cttcctccgt tcctgagttc
     6421 ccgacctccg tcctctacgt gatcggcgcc gacggcgagg gcgagcgccg cctcacggtc
     6481 gtcggcgagc cggcgacgat gccgtcctgg gggcctgccc gctgacctac gacgggagga
     6541 ggagcccgcc ggtcacgccg atcacctcgc cgacgacgaa gcgcgcctcg tcggacgcga
     6601 ggaagacgta ggcgggtgcg agctcggccg gctgcgccgg acgcccgagc gggttgtcct
     6661 gaccgaactc cgcgacctct tcc
//
LOCUS       DASTFC010000204         4957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_416196,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000204 DASTFC010000000
VERSION     DASTFC010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416196"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /gene="ftsW"
                     /locus_tag="VFO88_06110"
     CDS             complement(<1..1057)
                     /gene="ftsW"
                     /locus_tag="VFO88_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET9545132.1"
                     /translation="MKAARARGHLEFHLLVLVTLALVAFGLVMVYSASSGRAAVANGD
                     PAYYLKRQGLYALIGLVALAVLSRVRFPALRPLAAPFLVTAFGLLVAVLVVGTSVNGA
                     RRWLPLGVIDFQPSELAKLALAVWLAAFLARRPAPQTLGELFRPIGLVVLAACGLIVV
                     EPDLGTAIALVLMACAVLVVAGTPVRVLAGAASVLVALGLAAIWLEPYRRERLFSFMD
                     PWADPEGAGFQTVQAMIALGSGGLFGVGLGESVQKINYLPEATTDMIFAVVGEELGLV
                     GAAAVVGAFAVFGWAGFSVALKCRDPFGKFLATGITALVAGQALVNLAAVLGLAPLTG
                     IPLPFVSYGGTSLIVGLA"
     gene            complement(1054..2310)
                     /gene="murD"
                     /locus_tag="VFO88_06115"
     CDS             complement(1054..2310)
                     /gene="murD"
                     /locus_tag="VFO88_06115"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9545133.1"
                     /translation="MADPDIPRSVLVLGLARSGEAAALALAGRGVRVTAADRNEELDA
                     GRLRAAGVEVRLGADDPALLDGVDLLVKSPGVPTEAPLVAAARERAVPVWSEVELGYR
                     LLPGLPILGVTGTNGKTTTTELLGTIFRAAGRPVEVAGNVGRPLTAVADPSPDAWVVA
                     ELSSFQLEDVHDFHAAVAILLNVTPDHLDRHGTLERYTEAKLRVFENQREGDTAVVPR
                     GFRPIPGRATRVEFAVDDPLPAEPALRGEHNRENAAAATAAARAAGIPDHAIAEALRT
                     FTGIPHRLEPVGERNGVRFVNDSKATNPEAAARALTAYDRVRLIAGGSRKGTPFADLA
                     RAARERGVLRAYLIGESADELREAFAREGVPTSDAGDLETAVRAAAADAEPGDVVLLA
                     PACASFDQFRDYEERGERFRALVEAL"
     gene            2322..3032
                     /locus_tag="VFO88_06120"
     CDS             2322..3032
                     /locus_tag="VFO88_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9545134.1"
                     /translation="MRVAALFDVHGNLPALEAVLAEPDVAGADLVLCGGDTVPGPFSR
                     ECVVALDALGDRLVAVHGNGERNVVARDGEEARWCADELGAERLARLAALPTTAHVDI
                     SGLGRVLLCHGTPRSDEEIVTLVTPDERLGGILAGVEADVVLAGHTHTQIDRTVAGVR
                     WVNGGSVGMPYEDEPGARWALLTAAGVELRRTEYDREAAAERIRASEMPDADAFAAEY
                     VLAQHPPSETAPYFESLA"
     gene            complement(3029..4039)
                     /gene="mraY"
                     /locus_tag="VFO88_06125"
     CDS             complement(3029..4039)
                     /gene="mraY"
                     /locus_tag="VFO88_06125"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9545135.1"
                     /translation="MEKVLLSGILGMVISIAAGPKFIEFLQRNELGQHIREEGPAGHV
                     SKQGTPTMGGLLIMISMTVPFLIFTERTLPALTAFFVTLGCAAIGFVDDWTKLTHRRS
                     LGLAGRWKLLLLAVITGVVALVVYDADFKTSVYVPLANIDIPLSWAWYGLLFVIIAGA
                     ANGVNLTDGLDGLAAGTSTISLFTYTAMGVVAYLVSVRARSPNPEDLDLAILGASLIG
                     ASIGFLWYNAVPAQVFMGDTGSMGLGGALAAFAIMTKTEILLVLIGGVFVLEALSVII
                     QVLTFKFLGRRVFLMAPLHHHFEMKAWSETRIMVRFWIVGAILCACAFVLYYRYFSEF
                     AT"
     gene            complement(4057..>4957)
                     /gene="murF"
                     /locus_tag="VFO88_06130"
     CDS             complement(4057..>4957)
                     /gene="murF"
                     /locus_tag="VFO88_06130"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET9545136.1"
                     /translation="VVLEMGMRGVGQIAELCATARPDIGVITKIGPVHLELLGTLEAV
                     AEAKAELIRDLAPHGHAVVPAGDPLLEPHLEHVDAEILHFGPGGDVVLRTFAPHGDRS
                     TVELDAFGERLTLDLPFASRYNAENLLAALAAYRALGLPLDRAQEGASEIRLSRWRGE
                     ETELPGGGLLINDAYNANPLSMAAALEHLAERAAGRRTVACLGDMAELGPGAAAYHRE
                     VGAAAARVGVGVLVAIGPLARGYLEGARGIPETHWAHGLDDGLATLRRILRPGDCVLL
                     KASRAMGFEEIADALTVVSSEPA"
BASE COUNT          731 a         1744 c         1894 g          588 t
ORIGIN      
        1 cggcgagccc gacgatgagg ctcgtcccgc cgtagctcac gaacgggagc gggattccgg
       61 tgagcggcgc gagcccgagc acggccgcga ggttgacgag cgcctggccg gcgacgaggg
      121 ccgtgatccc ggtggcgagg aacttcccga acgggtcgcg gcacttgagc gcgacggaga
      181 acccggccca gccgaacacg gcgaaggcgc cgacgaccgc ggccgcgccg acgagcccga
      241 gctcctcgcc gacgacggcg aagatcatgt cggtcgtcgc ctcggggagg tagttgatct
      301 tctggacgct ctcgccgagc ccgacgccga agagcccgcc cgagccgagc gcgatcattg
      361 cctgaacggt ctggaagccg gcgccctcgg gatcggccca cggatccatg aacgagaaca
      421 ggcgctcgcg ccggtagggc tcgagccaga tcgccgcgag ccccagcgcg acgaggaccg
      481 aggcggcgcc ggcgaggacg cggaccggcg tccccgccac tacgaggacg gcgcaggcca
      541 tgaggacgag cgcgatcgcc gtcccgaggt ccggctcgac gacgatcagc ccgcacgcgg
      601 caaggacgac gagcccgatc gggcggaaga gctcgccgag cgtctgcgga gccggtcgcc
      661 gggcgaggaa cgcggcgagc cagaccgcga gcgcgagctt cgcgagctcg gacggctgga
      721 agtcgatcac gccgagcggg agccagcgcc gcgcgccgtt cacgctcgtc ccgacgacga
      781 ggacggccac gagcaggccg aacgcggtca cgaggaacgg ggcggcgagc gggcggagcg
      841 ccgggaagcg cacgcgcgag aggacggcga gcgcgacgag cccgatcagc gcgtagagcc
      901 cctggcgctt gaggtagtac gcgggatcgc cgttcgcaac cgcggcgcgg ccggagctcg
      961 cgctgtagac catgacaagc ccgaacgcga cgagcgcgag cgtcacgagg acgagcaggt
     1021 ggaactcgag gtgcccgcgg gcgcgcgcgg ccttcacagc gcctccacga gggcgcggaa
     1081 ccgctcgccg cgctcctcgt agtcgcggaa ctggtcgaag ctcgcgcacg cgggcgcgag
     1141 gaggacgacg tcgccgggct cggcgtcggc ggccgcggcg cggacggcgg tctcgaggtc
     1201 gcccgcgtcg ctcgtcggga cgccctcgcg cgcgaacgcc tcgcgcagct cgtccgcgct
     1261 ctcgccgatc aggtacgcgc ggaggacgcc gcgctcgcgc gccgcccggg cgagatcggc
     1321 gaacggcgtg cccttccgcg agccgccggc gatgaggcgc acgcggtcgt aggcggtgag
     1381 cgcgcgggcg gccgcctcgg ggttcgtcgc cttcgagtcg ttcacgaagc ggacgccgtt
     1441 tcgctcgccg accggctcga gccggtgcgg gatcccggtg aacgtgcgca gcgcctccgc
     1501 gatcgcgtgg tcggggatcc cggcggcgcg cgcggcggcc gtggcggcgg cggcgttctc
     1561 gcggttgtgc tccccgcgga gcgccggctc ggcggggagc gggtcgtcga cggcgaactc
     1621 cacgcgcgtg gcccggcccg ggatcggccg aaacccgcgc gggacgaccg ccgtgtcgcc
     1681 ctcgcgctgg ttctcgaaga cgcggagctt cgcctcggtg tagcgctcga gcgtgccgtg
     1741 ccggtcgagg tggtccggcg tgacgttcag gaggatcgcc acggcggcat gaaagtcgtg
     1801 gacgtcctcg agctggaagc tcgacagctc ggccacgacc cacgcgtccg gggacgggtc
     1861 ggcgacggcg gtgagcgggc gcccgacgtt cccggccacc tcgacgggac gcccggccgc
     1921 gcgaaagatc gtgccgagga gctcggttgt cgtcgtcttc ccattcgtcc ccgtgacgcc
     1981 gaggatcggg aggcccggca gcagccggta gccgagctcg acctcgctcc agaccggcac
     2041 ggcacgctcg cgcgcggcgg cgacgagcgg cgcctccgtc ggaactcccg ggcttttcac
     2101 gagcaggtcc acgccgtcga gaagcgcggg gtcgtccgcg ccgaggcgca cttcgacgcc
     2161 ggccgcgcga agcctccccg cgtcgagctc ctcgttccgg tcggccgccg tcacgcgcac
     2221 gccgcgcccg gcgagggcga gcgcagccgc ctcgccgctc cgggcgagcc cgagcacgag
     2281 gacgctccgc gggatgtcgg gatcggccat catcgtcgtc tgtgcgggtc gcagctctct
     2341 tcgacgtcca cgggaacctt cccgcgctcg aggccgtgct cgcggagccg gacgtcgcgg
     2401 gcgccgacct cgtcctctgc ggcggagaca ccgttccggg gccgttctcg cgcgagtgcg
     2461 tcgtggcgct cgacgcgctc ggcgaccggc tcgtcgcggt gcacggcaac ggcgagcgga
     2521 acgtcgtcgc gcgcgacggc gaggaggcgc gctggtgcgc ggacgagcta ggcgccgagc
     2581 ggctcgcccg gctcgccgcc ctgccgacga ccgcgcacgt cgacatctcc ggcctcggcc
     2641 gcgtcctcct ctgccacggc acgccgcgaa gcgacgagga gatcgtcacg ctcgtcaccc
     2701 cggacgagcg gctcggcggg atcctcgccg gcgtcgaggc ggacgtcgtg ctcgccggcc
     2761 acacgcacac gcaaatcgac cggaccgtcg cgggcgtccg ctgggtgaac ggcggcagcg
     2821 tcgggatgcc gtacgaggac gagcccggcg cacgctgggc gctcctcacc gccgccggcg
     2881 tcgagctccg ccgcaccgag tacgaccgcg aggccgccgc cgagcggatc cgcgcgagcg
     2941 agatgccgga cgccgacgcc ttcgccgccg agtacgtgct cgcccagcat ccgccgagcg
     3001 agacggcgcc gtacttcgag tcgctcgcct aggtcgcgaa ctccgagaag tagcggtagt
     3061 agaggacgaa ggcgcaggcg cagaggatcg cgccgacgat ccagaagcgc accatgatcc
     3121 gggtttccga ccaggccttc atctcgaagt ggtggtggag cggcgccatg aggaagacgc
     3181 gccgcccgag gaacttgaac gtgagcacct ggatgatcac cgagagcgcc tcgagcacga
     3241 agacgccgcc gatcaggacg agcaggatct ccgtcttcgt catgatcgcg aacgccgcga
     3301 gcgcgccgcc gagccccatc gagcccgtgt cacccatgaa gacctgcgcg gggaccgcgt
     3361 tgtaccagag gaacccgatc gaggccccga tgaggctcgc gccgaggatc gcgaggtcga
     3421 ggtcctccgg gttcggcgag cgggcgcgca cgctcacgag gtaggcgacg acgcccatcg
     3481 cggtgtacgt gaagagcgag atcgtcgacg tcccggcggc gagcccgtcg aggccgtccg
     3541 tcaggttcac gccgttcgcg gcgccggcga tgatcacgaa caggagcccg taccacgccc
     3601 acgagagcgg gatgtcgatg ttcgcgagcg ggacgtagac gctcgtcttg aagtccgcgt
     3661 cgtagacgac gagcgcgacg acgccggtga tcacggcgag caggaggagc ttccagcggc
     3721 cggcgagccc gagtgagcgg cggtgcgtga gcttcgtcca gtcgtccacg aacccgatcg
     3781 ccgcgcaacc gagcgtgacg aagaacgcgg tgagggcggg gagcgtccgt tccgtgaaga
     3841 tgaggaacgg gacggtcatc gagatcatga tcaggagccc gcccatcgtt ggggtgccct
     3901 gcttggacac gtggcccgcc ggcccctcct cgcggatgtg ctggccgagc tcgttccgct
     3961 ggaggaactc gatgaacttc gggccggccg cgatcgagat gaccatcccg aggatgccgg
     4021 agaggaggac cttttccacg cgcgcgcgtt atcccatcac gcgggctcgg acgacacgac
     4081 ggtcagggcg tcggcgatct cctcgaaccc catcgcacgc gacgccttga ggagcacgca
     4141 gtcgccgggc cggaggatcc gccgcagggt ggcgaggccg tcgtcgagcc catgcgccca
     4201 gtgggtctcg gggatgcccc gcgcgccttc gagatagccg cgggcgagcg ggccgatcgc
     4261 gacgaggacg ccgacgccga cgcgcgcggc cgcggcgccg acctcccggt ggtaggccgc
     4321 cgcgccggga ccgagctcgg ccatgtcccc gaggcaggcg accgtgcgcc ggccggccgc
     4381 gcgctcggcg aggtgctcga gggcggcggc catcgagagc gggttcgcgt tgtaggcgtc
     4441 gttgatgagg agcccgccgc ccgggagctc cgtctcctcc ccgcgccagc gcgagagccg
     4501 gatctcggag gcgccctcct gcgcgcggtc gaggggcagg ccgagcgcgc ggtaggcggc
     4561 gagcgcggcg aggaggttct ccgcgttgta gcgggacgcg aacgggaggt cgagcgtgag
     4621 ccgctcgccg aacgcgtcga gctcgaccgt cgagcggtcg ccgtgcggcg cgaacgtgcg
     4681 gaggacgacg tcgccgcccg gcccgaaatg gaggatctcc gcgtcgacgt gctcgaggtg
     4741 cggctcgagc agcgggtcgc cggccgggac gaccgcgtgg ccgtgcgggg cgaggtcgcg
     4801 gatgagctcc gcctttgcct cggcgaccgc ttcgagcgtc ccgaggagct cgaggtggac
     4861 ggggccgatc ttcgtgatca cgccgatgtc cggccgcgcg gtcgcgcaga gctcggcgat
     4921 ctggccgacg ccgcgcatgc ccatctcgag gacgacg
//
LOCUS       DASTFC010000205         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_432652,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000205 DASTFC010000000
VERSION     DASTFC010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432652"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFO88_06135"
     CDS             <1..390
                     /locus_tag="VFO88_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545137.1"
                     /translation="SEPMEFPAGVVCPFPVALESISGNESITFFASGRFHITGEGSTR
                     VTNLETQESIVLDTSGVVTFTPLEDGNLAITGSGRNLFYFLPQDVEGAGLFLITGRIS
                     EILDSSTDTITSSSSTGQRVDVCAALS"
     gene            493..1530
                     /locus_tag="VFO88_06140"
     CDS             493..1530
                     /locus_tag="VFO88_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9545138.1"
                     /translation="MQQLLVRMRELRLPRSRVAPTPFGADGLTVRLLRDKGSRRRLVV
                     NGLSASVAAVLLVFAVRHFSEKGWPLANANPRLAAAAGVLFLLAFGFKAFGWQRLFAP
                     DERPGRLALAAAAGAASVGGAALPGRVDDAIRVAFLRRYPGRKPGVATLCLSLFMLGL
                     VDTIALAPFASAAAVTSEVPLAVRIGLAIVATAGLGAAGVVLGLPRLGANRRLQRFRV
                     VRWLGSHVTCAREASAAAVFISLSWLVRAVGLLLLLGALGVGLSLPLALLFLCAAAAS
                     GALPVAPAGAATQAGAGAAVLVAAGLSTTQAIGFAVAAQVLVILAGAAVLLGALAWSG
                     GRVLVGRALAA"
     gene            1564..>2915
                     /locus_tag="VFO88_06145"
     CDS             1564..>2915
                     /locus_tag="VFO88_06145"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545139.1"
                     /translation="MATSAVEAKASLLDAAAGLVRERVEADRAPAVERFLRRYWEHVA
                     PEDLVGRTEDDLYGAAVAHWKLARVRQPGEILVRVYSPKLEEHGWECPHTVVETVTDD
                     MPFLVDSVSMEVLRHGHAVHLLVRPILRVARDDAGRIVDVQGDEGSDESLIHVELDRQ
                     SDPEELERLRADLVRVLGDVHAAVEDWPAMRERMLEAAAELAERPPPVEPDEAREATD
                     LLRWLHDDHFTFLGYREFEIVSESGADVLRGVPGTGLGVLRERGGPDVSTSFSELPPE
                     LRRIAREKTPLTLTKATSRATVHRPAYLDYVGVKRFGDDGEVTGERRFLGLYTHTAYR
                     ASPWEIPVLRRKVQRIVDRSGLPPAGHDHKALVEILETYPRDELFQAGEDELAQNAYG
                     IFRLGERARVRLFTRRDRFGRFVSCLVFLPRDRFHTENRRRIEEILLDAFGGVGADYT
                     T"
BASE COUNT          332 a         1111 c         1042 g          430 t
ORIGIN      
        1 agcgagccca tggagttccc ggccggcgtc gtctgcccgt tcccggtcgc tctcgagtcg
       61 atctcgggca acgagtcgat cacgttcttc gcgagcggcc ggttccacat caccggtgaa
      121 ggaagcaccc gcgtcaccaa cctcgagacg caggagtcga tcgtgctcga cacgtccggc
      181 gtcgtcacgt tcacgccgct cgaggacggc aacctcgcga tcaccgggag cggtcgcaac
      241 ctcttctact tcctgccgca ggacgtcgaa ggcgcgggcc tcttcctcat cacggggcgc
      301 atcagcgaga tcctcgactc ctccacggac acgatcacga gctcgagctc gaccggacag
      361 cgggtcgacg tctgcgcggc gctgagctag caacgcgaac cgcggcggtt caggaaccgg
      421 cgcgaggggg atgtgacgct gataccgtcc gccctcgcgc cggctcccgc cggccggggt
      481 ctagtagtcc tcgtgcagca gcttctcgtc cgcatgcggg agctccggct cccccgttct
      541 cgtgtcgcgc cgaccccgtt cggcgccgat ggcctgacgg tgaggctcct ccgggacaag
      601 ggctcgcgcc gccggctcgt cgtgaacggg ctctcggcct ccgtcgcggc cgtgctgctc
      661 gtcttcgccg tgcggcactt ctccgagaag ggctggccgc tcgccaacgc gaacccgcgc
      721 ctcgccgcgg cggccggggt gctcttcctc ctcgccttcg ggttcaaggc gttcggctgg
      781 cagcggctct tcgcgccgga cgagcggccc ggccggctcg cgctcgccgc ggcggccggc
      841 gcagcgtcgg tcggaggcgc cgcgcttccc ggccgcgtcg acgacgcgat ccgcgtcgcc
      901 tttttgcgcc gctacccggg ccgcaagccc ggcgtcgcga cgctctgcct ctcgctcttc
      961 atgctcgggc tcgtcgacac gatcgcgctc gccccgttcg cctcggcggc ggcggtgacg
     1021 agcgaggttc cgctcgccgt gcggatcggg ctcgccatcg tggcgaccgc ggggctcggc
     1081 gccgccggcg tcgtcctcgg gctgccgcgc ctcggcgcga accgccggct gcagcgcttc
     1141 cgcgttgtcc gctggctcgg gtcgcacgtg acctgtgcgc gcgaggcgtc cgcggcggcc
     1201 gtcttcatct cgctctcgtg gctcgtccgc gccgtgggcc tcttgctcct gctcggcgcc
     1261 ctcggcgtcg gcctctcgct cccgctcgcc ctgctcttcc tctgcgccgc ggccgcctcc
     1321 ggcgcgctgc cggtcgcacc ggcgggcgcg gcgacgcagg cgggcgccgg agcggcggtg
     1381 ctcgtcgccg cggggctctc gacgacgcag gcgatcggct tcgccgtcgc ggcccaggtg
     1441 ctcgtgatcc tcgccggggc cgccgtcctg ctcggcgcgc tcgcgtggag cggcgggcgc
     1501 gtgctcgtcg gccgcgcgct cgcggcgtaa cgccgctcgg ggtcatactc ccgggaggga
     1561 ggtatggcga cgagcgcagt ggaggcgaag gcgagtctgc tcgacgcggc ggccggcctc
     1621 gtccgcgagc gcgtcgaggc ggaccgcgcg cccgccgtcg agcggttcct gcgccgctac
     1681 tgggagcacg tcgcgcccga ggacctcgtc gggcggaccg aggacgatct ctacggcgcc
     1741 gccgtcgcgc actggaagct cgcgcgcgtc cggcagcccg gcgagatcct cgtgcgcgtc
     1801 tactcgccga agctcgagga gcacggctgg gagtgcccgc acacggtcgt cgagacggtc
     1861 accgacgaca tgccgttcct cgtcgactcc gtgtcgatgg aggtgctccg ccacgggcac
     1921 gccgtgcacc tcctcgtccg cccgatcctc cgcgtcgccc gcgacgacgc gggccgcatc
     1981 gtcgacgtcc agggcgacga ggggagcgac gagtcgctca tccacgtcga gctcgaccgc
     2041 cagagcgacc cggaggagct cgagcggctc cgcgccgatc tcgtccgcgt gctcggcgac
     2101 gtccacgcgg cggtcgagga ctggccggcg atgcgcgagc ggatgctcga ggcggccgcc
     2161 gagctcgccg agcgcccgcc gccggtcgag ccggacgagg cgcgcgaggc gaccgacctc
     2221 ctgcgctggc tccacgacga ccacttcacg ttcctcggct accgggagtt cgagatcgtg
     2281 agcgagagcg gggcggacgt gctgcgcggc gtgccgggca cggggctcgg cgtcctgcgg
     2341 gagcggggcg gccccgacgt gtcgacgagc ttctccgagc tgccgcccga gctccgccga
     2401 atcgcgcgcg agaagacccc gctcacgctg acgaaggcga cctcgcgggc gaccgtccac
     2461 cggccggcgt acctcgacta cgtcggcgtc aagcgcttcg gcgacgacgg cgaggtgacc
     2521 ggcgagcggc gcttcctcgg cctctacacg cacacggcgt accgcgcgag cccgtgggag
     2581 atccccgtgc tgcggcgcaa ggtgcagcgg atcgtcgacc gctccggcct cccgccggcc
     2641 gggcacgacc acaaggcgct cgtcgagatc ctcgagacgt acccgcgcga cgagctcttc
     2701 caggccggcg aggacgagct ggcgcagaac gcgtacggga tcttccggct cggggagcgg
     2761 gcgcgcgtgc ggctcttcac ccggcgcgac cgcttcggcc gcttcgtctc gtgtctcgtc
     2821 ttcctcccgc gcgaccgctt ccacacggag aaccggcgcc ggatcgagga gatcctcctc
     2881 gacgcgttcg gcggcgtcgg cgccgactac acgac
//
LOCUS       DASTFC010000206         7696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1157765,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000206 DASTFC010000000
VERSION     DASTFC010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157765"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1704)
                     /locus_tag="VFO88_06150"
     CDS             complement(286..1704)
                     /locus_tag="VFO88_06150"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HET9545140.1"
                     /translation="MRAPREPFRIKMVEPIRATTREEREAALAAAGWNVFQLAARDVE
                     LDLLTDSGTGAMSQAQWSALMLGDESYAGSESFQRFRAAVEDVLGFPFVVPAHQGRGA
                     EGVFFGAVIDEGEIVPSNAHFDTTRAHIEIRGGIALDFPVPEALDPGTWAPFKGDMNV
                     AGLQELLDGPDGERVSIVMLTITNNAGGGQPVSLANVRAVAEVARAHDKKLVLDIARF
                     AENAWFVKQREDGYADVPLPEIVRALVADADAVLMSAKKDAIANIGGFVAIREDVEFF
                     HRLQMRGIVSEGFPTYGGLAGRDLDAIAAGLREVLDEEYLRHRTEQVAFLGEALVEAG
                     AAVVQPVGGHAVYVDAGATLPHVPVDAFPAWALSCELYLAGAVRAAEIGSVMAGRDPE
                     TGENRHPPLELLRLAVPRRVYTSRQLEHAADAMREILRDPDAVRGYELVAEAPVLRHF
                     TARFAPLEERVAARAPAGPAGA"
     gene            complement(1712..2323)
                     /locus_tag="VFO88_06155"
     CDS             complement(1712..2323)
                     /locus_tag="VFO88_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HET9545141.1"
                     /translation="MDASPVELRVLGCLIEKQRTTPDAYPLSLNALRLACNQSTNRDP
                     VVDYDEETIRAALQRLGGRGWTRLASGAGSRAVKYRHLFGEALGLDDADLALLAVLML
                     RGPQTLGELKSRTERLHRFESIAAVEEALISLGERELVARLPRRPGQKEERFAHLLGG
                     DADEASAAAAPVPLPVADDRLTRLERRVDAIEEELAELRSRVG"
     gene            complement(2352..3422)
                     /gene="ftsZ"
                     /locus_tag="VFO88_06160"
     CDS             complement(2352..3422)
                     /gene="ftsZ"
                     /locus_tag="VFO88_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9545142.1"
                     /translation="MRDQGGSYLAVIKVVGIGGGGSNAVNRMVDAGLKGVEFIAINTD
                     AQALLMCDADVKLHIGAQATRGLGAGANPEVGRAAARETRDEIKESLKGADMVFVTAG
                     EGGGTGTGGAPVVAEVARELGALTVGVVTRPFAFEGRRRAEQAQMGISELQENVDTLI
                     VIPNDRLLQVVEQETSVVEAFRMADDVLRQGVQGITDLITVPGLVNLDFADVRTIMRD
                     AGSALMGIGEAEGPNRAAEAARQAVSSPLLEASIDGATGILLNISGGADIGLFEVNEA
                     AEVVTEAADQNSNVIFGAVIDDHLGGAVRVTVIATGFGDVRPRREKREQEREAEPVSE
                     GASRGFDVPQDILEVPSFLRDD"
     gene            complement(3492..4319)
                     /locus_tag="VFO88_06165"
     CDS             complement(3492..4319)
                     /locus_tag="VFO88_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9545143.1"
                     /translation="MAGRASMAVVRSPTLRPSTWPVGRRRVLGYALVALVGAALLYAG
                     ARETSAFAFRSVELEGARGAVAEDVRKALRTLEGESLVALDPGDVESLLRSLPAVRDA
                     RVDRAFPHTLSVVVEPERPLVVLEDGGRAWVVAESGRVIAAVEPGARPRLPRLLAEVA
                     EPPPVGASLGGIQMSETLTALAAIPKRFPGGVASAERGEAGLVLTLDTGLEVRLGEPV
                     DAEAKLAAAAAVIRALPDEELALYGYVDASVPARVVAGSEPQPSSDGLEFDDELPAN"
     gene            complement(4351..5250)
                     /gene="murB"
                     /locus_tag="VFO88_06170"
     CDS             complement(4351..5250)
                     /gene="murB"
                     /locus_tag="VFO88_06170"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET9545144.1"
                     /translation="MRPAALEEHVALSRYTTLGTGGPARWFAAPETLDGLLELLAWAA
                     AEDVPVAAVGLGSNLLVADEGFDGLALKLKGELASAEAVGERLVAGGGAPLAVCLHRA
                     RAAGLGGLEFACAIPGTIGGAVWMNAGAYGGDMAGVLERALVASASGAEWRTPAELGL
                     SYRRSGLRAGEVVARAELRLRPRPVEQIKATVAEMQGRRKAAQPTNKRTFGSVFKNPE
                     HELSAGRMLEACGLRGHAIGGATISPKHANFIENLGDARSADAVALIAEARRRALERF
                     GVRLEPEVQLLGEIAIPPFPDGT"
     gene            complement(5247..6458)
                     /locus_tag="VFO88_06175"
     CDS             complement(5247..6458)
                     /locus_tag="VFO88_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9545145.1"
                     /translation="MSPPLAGRRIWFVGIGGAGLSAYAILARAWGAEAAGWDRNETPY
                     LEPVRAAGIPVTIAPDAPPAPEGWETVVSTAYPAVAGTSRAAFLAELAGHARTIVVAG
                     AHGKTTTAGMIAYVLRELGLDPSWAIGGEVPQLGTHARAGSGWFVVEGDESDRTVASL
                     PADIAVVTNVDLDHHATFGSRAEVEELFAGWLAGLPDAHVVRGDEIEPLDVPLAVPGA
                     HNRRNAATALAALEAAGVAREDALGALRTFRGAGRRLEPRGEAGGVRVVDDYGHHPAE
                     VEASLRAARELAGGGRLLVLFQPHLYSRTRHLDRELAAALALADAACVADVYPAREAP
                     LPGVTGKLVVERLSELRPGMLVGWAPAVADGAAIVAAAARPGDLVVTVGAGNVDAAVP
                     LILERLGGSAP"
     gene            complement(6455..7522)
                     /gene="murG"
                     /locus_tag="VFO88_06180"
     CDS             complement(6455..7522)
                     /gene="murG"
                     /locus_tag="VFO88_06180"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET9545146.1"
                     /translation="MPQSSQPAFLIAAGGTAGHVLPALAVADALRRRGARVTFAGAQR
                     AEATLVPEAGYELDAFRVEGLPRRPGLALVRAVARAGAAVPACIRILRRRRPDVVLGG
                     GGYVAGPVALAARALRIPVALMEADAHLGLANRLSAPFARRVFLAFPLESRDGAKYRV
                     VGRPIPARSQPVPTEQARALFGLPAEGPVVLLFGGSQGAARLNEAAVEAWAERGPAVL
                     HLAGAAHAEGVAARVTRDGYVVLPFTDDFGAALAAADLVVARAGGSVWEVAAAGKPAL
                     LVPYPHATADHQTLNARFFERAGGAVVVPEPELDLAAQVDALLADPERLAAMGAAMAA
                     LARPHAAEVVADELLALAGAA"
BASE COUNT          974 a         3015 c         2794 g          913 t
ORIGIN      
        1 ccagcccgcg aacgggacga ggcgggcgcc gagcgcagcg tggcgctcgt ggagcggcgt
       61 gcggcggagc gtctgcaccg gagcggatcc tagaggctcg agccgcgcct gcggcggctt
      121 cgaagcgcgc gcagcctcgg acgcgcccga ccgccattcc ccgcccccag gggtggggtc
      181 ccggggaccg cgtcccgcct cccgcgttcg agcctaccgc ggaaatccgc acgggtccgt
      241 gccgattcgt ccacagcgtc ggcggcggcc gtgacgcgag cgccgtcagg cgcccgccgg
      301 cccggcgggg gcgcgcgccg cgacccgctc ctcgagcggg gcgaaccgcg cggtgaagtg
      361 ccgcagcacg ggcgcctccg cgacgagctc gtacccgcgc accgcgtcgg ggtcgcgcag
      421 gatctcccgc atcgcgtccg cggcgtgctc gagctgacgc gacgtgtaga cccggcgcgg
      481 caccgcaagc ctgagcagct cgagcggcgg gtgccggttc tccccggtct ccgggtcgcg
      541 cccggccatg accgacccga tctccgccgc ccgcaccgcg cccgcgagat agagctcgca
      601 cgagagggcc cacgcgggga acgcgtcgac cggaacgtgc gggagggtcg cgccggcgtc
      661 gacgtagacc gcgtgcccgc cgaccggctg gacgacggcc gcgccggcct cgacgagcgc
      721 ctcgccgagg aacgcgacct gctcggtccg gtgacgcagg tattcctcgt cgagcacctc
      781 ccggaggccc gccgcgatcg cgtcgaggtc gcgcccggcg agcccgccgt acgtcgggaa
      841 cccctcgctc acgatcccgc gcatctggag ccggtggaag aactccacgt cctctcgaat
      901 cgcgacgaac ccgccgatgt tcgcgatcgc gtccttcttc gcgctcatga gcaccgcgtc
      961 cgcgtcggcg acgagcgcgc gcacgatctc cggcagcggc acgtccgcgt acccgtcctc
     1021 gcgctgcttc acgaaccacg cgttctccgc gaagcgcgcg atgtcgagga cgagcttctt
     1081 gtcgtgcgcc cgcgcgacct cggcgaccgc gcggacgttc gcgagcgaga cgggctgccc
     1141 gccgcctgcg ttgttcgtga tcgtgagcat gacgatggag acgcgctcgc cgtccggccc
     1201 gtcgagcagc tcctggagcc cggcaacgtt catgtcgccc ttgaacggcg cccacgtccc
     1261 cgggtcgagc gcctccggca ccgggaagtc gagcgcgatc ccgccgcgga tctcgatgtg
     1321 cgcccgcgtc gtgtcgaagt gcgcgttcga cgggacgatc tccccctcgt cgatcaccgc
     1381 gccgaagaag acgccctccg cgccgcgccc ctggtgcgcc gggacgacga acgggaagcc
     1441 gagcacgtcc tcgaccgccg cgcggaaccg ctggaagctc tccgagcccg cgtacgactc
     1501 gtccccgagc atgagcgcgg accactgcgc ctggctcatc gcgccggtgc cggagtcggt
     1561 caggaggtcg agctcgacgt cgcgcgccgc gagctggaag acgttccagc cggccgccgc
     1621 gagcgccgcc tcgcgctcct cgcgcgttgt cgcgcgaatc ggctcgacca tcttgatccg
     1681 gaacggctcc ctcggcgcgc gcacgctgcc cctacccgac cctgctccgg agctcggcga
     1741 gctcttcctc gatcgcgtcg acgcgtcgct cgagccgggt gagccggtcg tccgcgacgg
     1801 ggagcgggac gggagccgcc gccgcgctcg cctcgtccgc gtcgccgccg agcaggtgcg
     1861 cgaaccgctc ctccttctgg cccggccggc gcgggagccg cgccacgagc tcgcgctcgc
     1921 cgagcgagat gagcgcttcc tccaccgccg cgatcgactc gaaccggtgc agccgctccg
     1981 tccgcgactt gagctcgccg agcgtctgcg gcccgcgcag catgagcacc gcgaggagcg
     2041 cgagatccgc gtcgtcgagg ccgagcgcct cgccgaagag atgccggtac ttcaccgcgc
     2101 gcgagccggc gccgctcgcg agccgcgtcc acccgcgccc gccgagccgc tggagcgcgg
     2161 cgcggatcgt ctcctcgtcg tagtcgacga cggggtcgcg gttcgtcgac tggttgcagg
     2221 cgagccgcag cgcgttcagc gagagcgggt aggcgtcggg cgtcgtccgc tgcttctcga
     2281 tcaggcagcc gagaacccga agctcgacgg gcgaggcgtc cacgcgcgaa gcgtaaacgc
     2341 ctccgggcgg ctcagtcgtc cctgaggaac gaggggactt cgaggatgtc ctgcggcacg
     2401 tcgaacccgc ggctcgcgcc ctcgctgacc ggctcggcct cgcgctcctg ctcgcgcttc
     2461 tcgcgccgcg ggcgcacgtc gccgaacccg gtcgcgatca ccgtgacgcg cacggcgccg
     2521 ccgaggtggt cgtcgatcac cgcgccgaag atcacgttcg agttctggtc ggccgcctcg
     2581 gtcacgacct ccgccgcctc gttcacctcg aagaggccga tgtcggcacc gccgctgatg
     2641 ttgagcagga tcccggtcgc gccgtcgatc gacgcctcga ggagcggcga ggagaccgcc
     2701 tgccgcgccg cctccgccgc gcggttcggg ccctccgcct cgccgatgcc catgagcgcc
     2761 gatccggcgt cgcgcatgat cgtgcgcacg tcggcgaagt cgaggttcac gagccccgga
     2821 acggtgatga ggtcggtgat cccctgcacg ccctgccgga ggacgtcgtc cgccatccgg
     2881 aacgcctcga ccaccgacgt ctcctgctcg accacctgga gaagcctgtc gttcgggatg
     2941 acgatcagcg tgtcgacgtt ctcctgcagc tcgctgatcc ccatctgcgc ctgctcggcg
     3001 cgccgccggc cctcgaaggc gaacggccgc gtgacgacgc cgaccgtgag cgcgccgagc
     3061 tcgcgggcga cctcggccac gacgggcgcg ccgccggtgc cggtcccgcc gccctcgccc
     3121 gcggtcacga agaccatgtc cgcgcccttg agcgactcct tgatctcgtc gcgggtctcg
     3181 cgcgccgcgg cgcggccgac ctccgggttc gcgccggcgc cgaggccgcg cgtggcctgc
     3241 gcgccgatgt ggagcttcac gtccgcgtcg cacatgagga gcgcctgcgc gtccgtgttg
     3301 atcgcgatga actcgacgcc cttgaggccc gcgtccacca tccggttgac cgcgttcgag
     3361 ccgccgccgc cgatgccgac gaccttgatg actgcgaggt agctgccgcc ttgatctctc
     3421 atggtcttct ccctgaacct ctacttgagc gtccgatgag cgtacgggcc gcgcacacgc
     3481 ctgtcaaatg gctaatttgc aggtagttcg tcgtcaaact ctaaaccatc acttgatggt
     3541 tgaggctccg agccggccac gacgcgcgcc gggacgctcg catcgacgta gccgtagagc
     3601 gcgagctcct cgtccggcag ggcccggatg accgccgccg cggcggcaag cttggcctcc
     3661 gcgtcgacgg gctcgccgag gcgaacctcg agcccggtgt ccagggtgag cacgaggccc
     3721 gcctcccctc gctcggcgga ggcgacaccg ccggggaagc gcttcgggat cgccgcgagg
     3781 gccgtgagcg tctcgctcat ctggatgccg ccgagggagg cgccgacggg cggcggctcg
     3841 gcgacctcgg cgaggaggcg cggcaggcgg gggcgggcgc ccggctcgac cgcggcgatc
     3901 acacgaccgc tctcggccac gacccaggcc cgcccgccgt cctcgaggac gacgagcggc
     3961 cgttccggct cgacgacgac ggagagggtg tgcgggaacg cccggtcgac ccgcgcgtcg
     4021 cgcactgccg gaaggctgcg caggaggctc tccacgtcgc cggggtcgag cgcgacgagg
     4081 ctctcgccct cgagcgtccg gagcgccttg cgcacgtcct cggccacggc gccccgcgcg
     4141 ccctcgagct cgacggagcg gaaggcgaac gccgaggtct cgcgcgcgcc cgcatagagg
     4201 agcgccgcgc ccaccagcgc gacgagcgcg tagccgagca cgcggcggcg ccccaccggc
     4261 caggtggacg ggcggagggt cggcgagcgc acgacggcca tggaggcgcg gcctgccacc
     4321 gcgtcccctt cgccgcggcg cgtcgcggtc ctacgtcccg tccgggaagg gcgggatcgc
     4381 gatctcgccg aggagctgca cctccggctc gagccgcacg ccgaaccgct cgagcgcccg
     4441 ccggcgcgcc tcggcgatca gcgccaccgc gtcggcggag cgcgcgtcgc ccaggttctc
     4501 gatgaagttc gcgtgcttcg gggagatcgt cgcgccgccg atcgcgtgcc cgcgcaggcc
     4561 gcacgcctcg agcatccgcc cggccgaaag ctcgtgctcc gggttcttga agacgctccc
     4621 gaacgtccgc ttgttcgtcg gctgcgccgc cttgcgcctg ccctgcatct ccgccacggt
     4681 cgccttgatc tgctcgaccg gccgcgggcg gaggcgaagc tcggcgcggg cgacgacctc
     4741 gccggcgcgc aggccggagc ggcggtagct gagcccgagc tccgcgggcg tccgccactc
     4801 ggccccgctc gccgacgcga cgagcgcccg ctcgaggact ccggccatgt cgcctccgta
     4861 cgcgccggcg ttcatccaga ccgctccgcc gatcgtgccg gggatcgcgc acgcgaactc
     4921 gaggccgccg agcccggccg cccgcgcgcg gtggagacag acggcgagcg gcgcgccccc
     4981 gcccgcgacg aggcgctcgc ccaccgcctc ggccgacgcg agctcgccct tcagcttgag
     5041 cgcgagcccg tcgaacccct cgtccgcgac gagcaggttc gagccgagcc cgaccgccgc
     5101 gaccgggacg tcttccgccg ccgcccaggc gagcagctcc aggagcccgt cgagcgtctc
     5161 gggcgccgcg aaccagcgcg cgggcccgcc ggtgccgagc gtcgtgtagc gcgagagcgc
     5221 gacgtgctcc tcgagcgcag ccggcctcac ggcgcggagc ctccgagccg ctcgaggatg
     5281 agcgggacgg ccgcgtcgac gttccccgcg ccgacagtca cgacgaggtc gcccggccgc
     5341 gccgccgcgg ccacgatcgc cgcgccgtcg gcgaccgcgg gcgcccaccc gacgagcatt
     5401 cccggccgga gctcgctcag ccgctcgacg acgagcttcc cggtcacgcc ggggagcggc
     5461 gcctcgcgcg cgggatagac gtccgcgacg caggccgcgt cggcgagcgc gagcgccgcg
     5521 gcgagctctc ggtcgaggtg ccgcgtccgc gagtagaggt ggggctggaa gaggacgagg
     5581 aggcggccgc cgcccgccag ctcgcgcgcc gcgcgaagcg acgcctcgac ctccgcggga
     5641 tggtggccgt agtcgtcgac gacccgcacg ccgcccgcct ccccgcgcgg ctcgagcctg
     5701 cgccccgcgc cgcggaacgt gcgcagcgcc ccgagcgcgt cctcgcgcgc gacccctgcg
     5761 gcctcgagcg cggcgagcgc ggtcgccgcg ttgcggcggt tgtgcgcccc ggggacggcc
     5821 agcggcacgt cgagcggctc gatctcgtcg ccgcgcacga cgtgcgcgtc cggcaggccg
     5881 gcgagccagc cggcgaagag ctcctccacc tccgcgcgcg agccgaacgt cgcgtggtgg
     5941 tcgaggtcga cgttcgtgac gaccgcgatg tcggcgggaa gcgacgcgac cgtccggtcg
     6001 gactcgtcgc cctcgacgac gaaccagccg ctcccggcgc gcgcgtgcgt cccgagctgc
     6061 gggacctcgc cgccgatcgc ccaggaggga tcgagcccga gctcgcggag gacgtacgcg
     6121 atcatccccg cggtcgtcgt cttcccgtgc gcgcccgcga cgacgatcgt gcgcgcgtgg
     6181 ccggcgagct cggccaggaa ggccgcgcgg ctcgtccccg cgaccgcggg atacgcggtc
     6241 gagaccacgg tctcccagcc ttccggcgcc ggcggcgcgt cgggcgcgat cgtgaccggg
     6301 atccccgccg cgcgcacggg ctcgaggtat ggcgtctcgt tccggtccca cccggccgcc
     6361 tcggcgcccc acgcgcgcgc gaggatcgcg tacgccgaga gcccggcgcc gccgatcccc
     6421 acgaaccaga tcctccggcc ggcgagcgga gggctcacgc cgcccccgcg agcgcgagga
     6481 gctcgtcggc cacgacctcg gccgcgtgcg gccgggcgag cgccgccatc gccgcgccca
     6541 tggcggccag ccgctccggg tcggcgagca gcgcgtccac ctgcgccgcg aggtcgagct
     6601 cgggctccgg cacgacgacc gcgccgccgg cgcgctcgaa gaagcgggcg ttcagcgtct
     6661 ggtggtccgc ggtcgcgtgc gggtacggga cgaggagcgc cggcttcccg gccgcggcca
     6721 cctcccagac cgagccgccc gcccgcgcca cgacgaggtc ggcggcggcg agcgcggcgc
     6781 cgaagtcgtc cgtgaacggg agcacgacgt acccgtcgcg cgtaacgcgt gccgcgacgc
     6841 cctccgcgtg cgccgcgccg gccaggtgga ggactgcagg cccccgctcg gcccacgcct
     6901 ccaccgccgc ctcgttcagc cgcgccgcgc cctggcttcc cccgaagagg agcacgaccg
     6961 gcccctccgc cggaagcccg aagagcgcgc gagcctgctc ggtcgggacc ggctgcgagc
     7021 gcgccgggat cggccgcccc acgacgcggt acttcgcccc gtcgcggctc tcgagcggga
     7081 acgcgaggaa gacgcgccgg gcgaacggcg cggagagccg gtttgcgagc cccaggtgcg
     7141 cgtcggcctc catgagcgcg accgggatcc gcagggcgcg ggccgcgagc gcgaccggcc
     7201 cggcgacgta gccgccgccg ccgaggacga cgtccggccg gcggcggcgc aggatccgga
     7261 tgcacgccgg caccgccgcg cccgcccgcg ccaccgcccg cacgagcgcg agcccgggcc
     7321 ggcgcgggag tccctcgacc cggaacgcgt cgagctcgta cccggcctca gggacgagcg
     7381 tcgcctcagc ccgctgcgcg ccggcgaacg tgacccgcgc gccgcgccgg cgcagcgcgt
     7441 ccgccaccgc gagcgcgggc aggacgtgcc cggccgtccc tccggcggcg atcaggaacg
     7501 cgggctggct cgactgcggc atggttgacc gcgatgttaa ggaggatgcc gaccgcggcg
     7561 agcccgacga tgaggctcgt cccgccgtag ctcacgaacg ggagcgggat tccggtgagc
     7621 ggcgcgagcc cgagcacggc cgcgaggttg acgagcgcct ggccggcgac gagggccgtg
     7681 atcccggtgg cgagga
//
LOCUS       DASTFC010000207        13154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_515313,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000207 DASTFC010000000
VERSION     DASTFC010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515313"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFO88_06185"
     CDS             complement(<1..291)
                     /locus_tag="VFO88_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9545147.1"
                     /translation="MLGYAFMGKAHSRAFREADAFRAPLSPRLVSLSGRTAEKVEAAR
                     EELGWAEAVTDWREQVADERIGLFDNGGPNALHAEPTIAAARNGKHVLCEKPL"
     gene            complement(318..1616)
                     /locus_tag="VFO88_06190"
     CDS             complement(318..1616)
                     /locus_tag="VFO88_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545148.1"
                     /translation="MSAEPPVEAEAHIDPTAARVEHVPAGEQVDETRASETLSGYLRL
                     WWQGVRAGELGSLPIIVGLIVIFITFGILEDNFLTERNFTNLILQMAAVATIAIGVVF
                     VLLIGEIDLSVAYVSAVGAVVMTLLLRPDDPGWPWWLAIAFALLCTTTIGFLQAIVIT
                     KAGVPSFVVTLAGFLIWSGVVLILTTQYSTAGTIRIQNGTVVGIANDFLSELWGWILG
                     ILVIAAYALLQLRTAITRRARGLAAKPVVLVAIQTLLLAAVTFAAVWFLNKDRGVPKV
                     AVILGILLVFWTFVASRTRFGRHVYAVGGSAEAARRAGINVDRVRIAVFMISGFMAGV
                     GGIMLASRLRSVATNTGGGNLLLLIIAAAVIGGTSLFGGVGRVVSALLGALVIASIQN
                     GMDLLGLASGTKFVITGLVLLGAVLVDAFSKRRRAARGGV"
     gene            complement(1613..2392)
                     /locus_tag="VFO88_06195"
     CDS             complement(1613..2392)
                     /locus_tag="VFO88_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9545149.1"
                     /translation="MADTPLLECRGASKAFGAVQALYRVDFEVRPGEVMALVGDNGAG
                     KSTLIKGVAGIYPFDEGEVYVEGKQVHIHGPRDAARLGIEVVYQDLALADNLDVVANM
                     YLGRERVRNGLVLDESNMERSARETLDSLSVTTLRSVRQAVAGLSGGQRQSVAVAKAV
                     MWNSRVVILDEPTAALGVVQTRQVLNLVRRLAEKDLGVALISHNLHDIFEVADRISVL
                     RLGQLVGVYERASTTQEEVVHAITAGKLTHVPGMEEVEVQA"
     gene            complement(2462..3619)
                     /locus_tag="VFO88_06200"
     CDS             complement(2462..3619)
                     /locus_tag="VFO88_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9545150.1"
                     /translation="MLLALIFALGAVAAGCGGDDDDGDDTAATEAEGDGEAAGEGSIW
                     VLLPDSASSPRWESDDRRFFEQFFSEAGVEYTIVNAEGDAAQQQSQAEQAIADGASVI
                     LLTSLDTGSGAAIIDQAKDAGVEVVEYDRFNTGGSGGAAYVSFDNVQVGATMAEELEP
                     AIDDLDAETPRVVMLNGGEEDNNSFLFRQGYAETVEARVDAGDWELVADQHVPGWDNQ
                     EAQTIMEQILTSANNEVDAVFAANDGLANSAINALEAAGVEGAPVSGQDATVAGIQNI
                     LLGKQTMTVYKPIEAEAAVAAAVALALRAGEDVSSVESEYEFELIGITAEDGQPADSP
                     EGEGVVPYFALVPIAVTVDNIQETVIEDGFRTIEEVCTGDTAQTDFCQENS"
     gene            3849..5078
                     /locus_tag="VFO88_06205"
     CDS             3849..5078
                     /locus_tag="VFO88_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET9545151.1"
                     /translation="MTRPATPRDTNRLRILDTLRREGRASRGELARMTGLSRSTVAGL
                     VGELARQGLVVDGGEPEEAGALAGPGRPPILLRLGAAAGTAVGIDFGHGHVRVAVADL
                     SSEVLAERTVDLDVDHEAAHALDVGAELVTQVLAEARVERGTVLGAGVGVPAPIDRAT
                     GTLRSSSIIPGWAGLDLAGELGARLGMPVAIENDANLGALAEVSFGAGRGLEDVVYVR
                     CERGIGAGLFLGGALYRGSSGLAGELGHVQVRAEGAVCRCGNRGCLETVAATGALLAA
                     LEPAHGHDIGVRRLRELVEAGDPGATRVVNDAGRAVGRVLADLVNHLNPAAIIVGGAL
                     VGLAAEPLLQGIRDTVDRYAQPGTAEAVDVRPGVLGERAEVLGALALVIRDTERIGSG
                     TLVSLAREAAPALAGGR"
     gene            complement(5075..5527)
                     /gene="greA"
                     /locus_tag="VFO88_06210"
     CDS             complement(5075..5527)
                     /gene="greA"
                     /locus_tag="VFO88_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET9545152.1"
                     /translation="MTEHGERLTEAQKTRLEEELAELEGPKRAEIVEAIKTARSFGDL
                     SENYEYHAAKNEQGLLERRITILRDRLHRAVVVDGAGADDGRVAVGSHVTIARADDGE
                     EMEVEISSVGGVSPDSPLGRALLGAHVGDEVEIEAPKGAWRARVVSVR"
     gene            complement(5538..6077)
                     /locus_tag="VFO88_06215"
     CDS             complement(5538..6077)
                     /locus_tag="VFO88_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545153.1"
                     /translation="MRADGLAVLRSILPLAAAVARGTRKLALVALAAAAVIALVVLLR
                     WTPDEDETWVGAVILLLLLTVPGGVLLFFSFVLGEVVELPEKLLRSPQTAREHAGELR
                     AITRDAQSRQAPAWRRLPGSTWRLAGLVRSARDLLAPHAPILPLLSIPFLAAAAISAL
                     VTPLLVLAALVVLLAAAAG"
     gene            complement(6074..7660)
                     /locus_tag="VFO88_06220"
     CDS             complement(6074..7660)
                     /locus_tag="VFO88_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET9545154.1"
                     /translation="MRGDLDRRLEALAEASGLAEGRLPDEAVERARAVVARAGERLGL
                     GLESTVVALAGPTGAGKSRLFNALAGEDLAAVGRRRPTTSTGQAAVWGDGAGPLLDWL
                     EIPRRHRIAPDGLDGLVLLDLPDFDSVETAHRLEVDRVVGLADLVAWVVDPQKYADAA
                     LHDRYLRPLASHAEAMAVVLNQADLLSPADVERWRGDLERLLAEDGVDGAPVVVVSAE
                     TGAGLDDLRRLLADRVRARDAAIARLAADVDDAVLPLAVACGEDGAAGVQRDDRRRLL
                     AALEDAAGVPAVLRAVEAAHRRRGALGTGWPFVRWVRRFRPDPLRRLRLPERPQPAVR
                     TSLPRPTDVQTAQVATAARRLADRAAAGLPEPWPRLAREAATARDEEVADRLDGAVSG
                     ADLHVSRPRWWRAVSLLQTGLALAVAAGALWLAVLLVLGYLRVEDVVPLPEAYGIPVP
                     TWLLVGGALAGLALAFLARLVNGAAARRRARAASRSLRAGVEEVGQELVIAPVEAELE
                     AAGRLCAAVAVARGRKRRRR"
     gene            complement(7657..9336)
                     /locus_tag="VFO88_06225"
     CDS             complement(7657..9336)
                     /locus_tag="VFO88_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HET9545155.1"
                     /translation="MSNGLPAALGRLRDDLAAVRLRLDVPGAEEARRVRDDLVAQVDD
                     YLLPRLDRMDAPALIVVGGSTGAGKSTLVNSLVGEEISPAGVLRPTTRAPVLAFHPDD
                     LAWFEGPRVLPGLPRASGGDAAPGTLALAPTAALPPGLALLDSPDIDSVLAENRALAN
                     QLLAAADGWLFVTTAARYADAVPWELLRAARDRGTALSLVLNRVPADAGREVPEHLGR
                     MLRDERLAEVDLLVVPETELADGLLPEGALAPARAWLEDLAADAQARAALVRRTLRGA
                     LASLPARAAVVERAVMEQISVAADLRAEADAAYVDALHEVEAALRSGSLLRGEVLARW
                     HEVVGTGDVMRALETGIGKARDRLRSLVTGRPAGDEELREAVENRVEAVVRAAAERAA
                     ERTARAWRERVPGRALLEGTTGLDRASAELEPAAEEEVRAWQGVVFDLVREEGASKRA
                     TARLASLGVNGAGLTVMLAVFAHTGGLTGTEVVVAGGTSAVGQKVLEAIFGDQAVRTL
                     AARAREDLVERVDGLLRAEAARFDALLDEAAPEADTFARLHEAVDAIRRAA"
     gene            9473..11389
                     /locus_tag="VFO88_06230"
     CDS             9473..11389
                     /locus_tag="VFO88_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545156.1"
                     /translation="MHARALRAAVIAAALAVLALPATAGAQGHEMPPSGPGFLEGPGE
                     WGKIELVSTEFVTDQDDLVADVALDPSHDYAYLAHWGEPDCAAPSEGGGKNAPDAGVW
                     VIDITDLENPEEVGFIPSSQDSRPGEGMQAVEVSSKFFTGTLLATNNEQCGPQGKGGV
                     TLYDVTNPLKPKLLSKHFGDRGFADANDIHNVFVWDAGENVYAVMVDNFETTDVDILD
                     ITNPKRPRLIADINLNEYIEGGVDQPELGLTDSFLHDMVVKQIDGRWIMLLSYWDGGF
                     VMLDVTDPANPVYILDTEFAEIDPELLESAGISLTPEGNAHQAEFTIDNRFFIGGDED
                     FAPYRTDEFEITSGPYAGVYPSVIVPGGQAPAILPDLTLNGPVVYGGYGCPSSAPLPS
                     PEDIPGYLDMLEPDEETIIVLQRGPVGDPSAPEEACFPGEKAHEAALAGWDAVLFVNH
                     HSGEAAGGEPFCGSGAFVDNIVAVCTTHAAFHRLFDLQPLDPPWTYPENIAIGAIGSE
                     IEVGSLFDGWGYVHLYDAETGAELDTYAIPEAHDPMFSLGFGDLTVHEVATDPQDASL
                     AYLSYYSGGLIALQIQCTNPNDNSTCELVEVGGYRDPMGNDFWGVETFVGEDGFTYIL
                     ASDRDSGLWIFRDP"
     gene            complement(11471..11785)
                     /locus_tag="VFO88_06235"
     CDS             complement(11471..11785)
                     /locus_tag="VFO88_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545157.1"
                     /translation="MYARVTTFEGSPEDIEAGVRVYREDVIPWLRDAEGFRGWIVLLD
                     REAGRSIGLTFWTSAEAALNREQSGAALRDEVAEAVGATMRSLELYEVVAADELALDE
                     LR"
     gene            complement(11796..>13154)
                     /locus_tag="VFO88_06240"
     CDS             complement(11796..>13154)
                     /locus_tag="VFO88_06240"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET9545158.1"
                     /translation="RALAPEGHRRMGANPHANGGILLRPLELPDFRDYAVEVETPART
                     ASEPTRVLGTYLRDVVRLNEDRFRVFGPDETASNRLSALFEATDRAWMAERKTTDDNL
                     APDGRVLEILSEHLCQGWLEGYLLTGRHGLFNCYEAFIHIIDSMFNQHAKWLKVTRGI
                     PWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVVNKKAEIIRVYLPPDANCLLSV
                     ADHCLKSRHYVNVIVAGKQPSLDYLTMDEAVAHCTRGIGTWDWASTDEGGEPDVVLAC
                     AGDIPTLETLAAASLLRRHLPDLKVRVVNVVDLMRLQDEREHPHGLPDAEFDALFTTS
                     RPIVFAYHGYPWLVHRLTYRRTNHANLHVRGYKEEGTTTTPFDMVMLNDLDRFHLVMD
                     VIDRVPGLGERAAHLRQEMVDERLRHRAYTRETGDDMPGVRDWVWEPAAAADGQPRGE
                     RA"
BASE COUNT         1799 a         4806 c         4813 g         1736 t
ORIGIN      
        1 gagcggcttc tcgcagagga cgtgcttgcc gttgcgcgcg gcggcgatcg tcggctcggc
       61 gtggagggcg ttcgggccgc cgttgtcgaa gaggccgatg cgctcgtcgg ccacctgctc
      121 gcgccagtcc gtgacggcct cggcccagcc cagctcctcg cgcgcggcct cgaccttctc
      181 ggcggtgcgc ccggagagcg agacgagccg cggcgagagg ggcgcgcgga aggcgtcggc
      241 ctccctgaac gcacgcgagt gcgccttgcc catgaaggcg tatccgagca tgccgacgcc
      301 gatggtctcg cgggtcgtca cacgccgccg cgagcggccc ggcggcgttt ggagaaggcg
      361 tccacgagca ccgcaccgag caggacgagg ccggtgatga cgaacttcgt gccggacgcc
      421 aggccgagca ggtccatgcc gttctggatc gaggcgatga cgagggcccc gagcagcgcc
      481 gagacgacgc ggccgacgcc cccgaagagg ctcgtcccgc cgatgacggc cgccgcgatg
      541 atgagcagga ggaggttgcc gccgcccgtg ttggtggcga cggagcgcag ccgcgaggcg
      601 agcatgatcc cgcccacgcc ggccatgaag ccgctgatca tgaagacggc gatgcgcacg
      661 cggtcgacgt tgatgcccgc gcgccgcgcg gcctctgcgc tgccgccgac cgcgtagacg
      721 tggcggccga accgcgtgcg tgaggcgacg aacgtccaga agacgagcag gatcccgagg
      781 atgaccgcga ccttgggcac gccgcggtcc ttgttcagga accagacggc ggcgaacgtc
      841 accgcggcga ggagcagcgt ctggattgcc acgagcacca ccggcttcgc ggccaggccg
      901 cgcgcccgcc gggtgatcgc cgtgcgcagc tggaggaggg cgtaggccgc gatcacgagg
      961 atcccgagga tccagcccca gagctcgctc aggaagtcgt tcgcgatccc gacgacggtg
     1021 ccgttctgga tccggatcgt cccggccgtc gagtactggg tcgtgaggat caggaccacg
     1081 ccggaccaga tgaggaaccc ggccagggtg acgacgaacg agggaacgcc ggctttcgtg
     1141 atgacgatcg cctgcaggaa cccgatcgtc gtcgtgcaga ggagcgcgaa cgcgatcgcg
     1201 agccaccacg gccagcccgg gtcgtcgggg cgcaggagga gcgtcatcac caccgcgccc
     1261 accgcgctca cgtaggcgac ggaaaggtcg atctcgccga tcaggaggac gaagacgacg
     1321 ccgattgcga tcgtcgcgac ggccgccatc tggaggatca ggttcgtgaa gttccgctcg
     1381 gtgaggaagt tgtcctcgag gatcccgaac gtgatgaaga tgacgatcag cccgacgatg
     1441 atcgggagcg agccgagctc gcccgcgcgg acgccctgcc accagaggcg gaggtagccg
     1501 gacagcgtct cggacgcgcg tgtctcgtcg acctgctccc cggccgggac gtgctcgacg
     1561 cgcgcggcgg tcgggtcgat gtgcgcctcg gcctcgaccg ggggctcggc gctcatgcct
     1621 gaacctccac ctcctccatc cccggcacgt gcgtcagctt cccggctgtg atggcgtgca
     1681 cgacctcctc ctgcgtcgtc gaggcgcgct cgtatacccc tacgagctgg ccgagccgca
     1741 ggacgctgat cctgtcggcg acctcgaaga tgtcgtgcag gttgtgcgag atcagcgcga
     1801 caccgaggtc cttctccgcg aggcggcgca cgaggttgag cacctggcgc gtctgcacga
     1861 ccccgagcgc cgcggtcggc tcgtcgagga tcacgacccg tgagttccac atgacagcct
     1921 tggccacggc cacggactgc cgctggccgc cggagaggcc tgcgactgcc tggcgcaccg
     1981 agcggagcgt cgtcacggag aggctgtcca gcgtctctcg cgccgaccgt tccatgttcg
     2041 actcgtcgag gacgaggccg ttcctgacgc gctcgcggcc gaggtacatg ttggcgacga
     2101 cgtccaggtt gtccgccagc gcgaggtcct ggtagacgac ctcgatcccg aggcgggcgg
     2161 cgtcgcgcgg cccgtggatg tgcacctgct tcccctcgac gtacacctcg ccctcgtcga
     2221 acggatagat gccggcgacg cccttgatga gggtcgactt tcctgcaccg ttgtccccga
     2281 cgagcgccat cacctcgccg ggccgcacct cgaagtcgac ccggtagagc gcctgaacgg
     2341 ccccgaatgc cttcgaggcg cccctgcact cgaggagcgg cgtgtcagcc atgccagatc
     2401 ttctcacgtc ggcggcggag ggggcgcctg ggcggacgcc ccctccctgc cgttcagcgg
     2461 gtcagctgtt ctcctggcag aagtcggtct gcgcagtgtc gccggtacag acctcctcga
     2521 tcgtccggaa gccgtcttcg atgaccgttt cctggatgtt gtccacggtg accgcgatcg
     2581 ggacgagcgc gaagtacggg acgacgccct cgccctcggg cgagtcggcc ggctgcccgt
     2641 cctcggcggt gatgccgatc agctcgaact cgtactccga ctcgacggag gagacgtcct
     2701 cgccggcccg cagcgcgagc gcaacggccg cggcgacagc cgcctcggcc tcgatcggct
     2761 tgtagacggt catcgtctgc ttgccgagga ggatgttctg gatcccggcg accgtcgcgt
     2821 cctggccgct caccggcgcg ccctcgacgc cggccgcctc cagcgcgttg atcgcggagt
     2881 tcgcgagccc gtcgttcgcg gcgaagaccg cgtcgacctc gttgttcgcg ctcgtcagga
     2941 tctgctccat gatcgtctgg gcttcctggt tgtcccagcc cgggacgtgc tggtcggcga
     3001 cgagctccca gtcacccgcg tcgacgcgcg cctcgacggt ctcggcgtag ccctggcgga
     3061 agaggaacga gttgttgtcc tcctcgccgc cgttcagcat gacgacgcgc ggcgtctcgg
     3121 catcgaggtc gtcgatcgcc ggctcgagct cctcggccat cgtcgcgccg acctgcacgt
     3181 tgtcgaagct cacgtacgcg gcgccgccgg agccgcccgt gttgaagcgg tcgtactcga
     3241 ccacctcgac gcccgcgtcc ttcgcctggt cgatgatcgc ggcgcccgag cccgtgtcga
     3301 ggctcgtgag gaggatgacg ctcgcgccgt ccgcgatcgc ctgctccgcc tgcgactgct
     3361 gctgggccgc gtcgccctcg gcgttcacga tcgtgtactc gaccccggcc tcgctgaaga
     3421 actgctcgaa gaagcgccgg tcgtccgact cccagcgggg ggagctcgcg ctgtcgggga
     3481 ggagcaccca gatcgagcct tcgcccgctg cctctccgtc gccttcggcc tcggtcgctg
     3541 ccgtgtcgtc gccgtcgtcg tcgtcaccgc cgcacccggc cgccacggcg ccgagcgcaa
     3601 agatgagagc gaggagcatg acgacgaggc gccacctcgc tccgtttccg tccgatccga
     3661 acacgagtgt cctcactttc ttctcgacag cacgcgaccg cgggagccgc gtgccggaac
     3721 cgtaggtgcc atgccgtccg aatgcaagcg atcgggggtg gaattcgggc cgaatccggg
     3781 cgaaaccacg gggtagccct caccgtttac tccccatcgt gcgcgaagtg cgaaagaatc
     3841 gggccgcgat gacgaggccg gcgaccccgc gggacacgaa ccggctgcga atcctcgaca
     3901 cgctccgccg ggagggccgc gcgagccgcg gcgagctcgc ccgcatgacg ggcctctcgc
     3961 gctcgacggt ggcgggcctc gtcggcgagc tcgcgcgcca gggtctcgtc gtcgacggcg
     4021 gcgagcccga ggaggccggg gccctcgcgg ggcccggccg cccgccgatc ctcctccgcc
     4081 tcggcgcggc cgcggggacg gccgtcggga tcgacttcgg ccacggacac gtgcgcgtgg
     4141 ccgtcgccga cctctcgtcg gaggtcctcg ccgagcggac ggtcgacctc gacgtcgacc
     4201 acgaggccgc gcacgcgctc gacgtcggcg cggagctcgt gacccaggtc ctcgcggagg
     4261 cgcgcgtcga gcgcgggacc gtcctcggcg cgggcgtcgg cgtgccggcg ccgatcgacc
     4321 gcgcgaccgg gacgctccgg tcgtcgtcga tcatcccggg ctgggcgggg ctcgacctcg
     4381 ccggcgagct gggcgcccgc ctcggcatgc cggtcgcgat cgagaacgac gcgaacctcg
     4441 gcgcgctcgc cgaggtgtcc ttcggcgccg ggcgcgggct cgaggacgtc gtctacgtgc
     4501 gctgcgagcg cgggatcggc gccgggctct tcctcggcgg cgcgctctac cgcggctcgt
     4561 ccggcctcgc cggggagctg ggccacgtgc aggtccgcgc ggagggcgcc gtctgccggt
     4621 gcggcaaccg gggctgtctc gagacggtcg cggcgacggg cgcgctcctc gccgcgctcg
     4681 agcccgcgca cgggcacgac atcggcgtgc gccgcctgcg cgagctcgtc gaggcgggcg
     4741 acccgggcgc gacgcgtgtc gtgaacgacg cgggccgcgc ggtcggccgg gtcctcgccg
     4801 acctcgtgaa ccacctgaac ccggccgcga tcatcgtcgg cggcgcgctc gtcgggctgg
     4861 ccgcggagcc gctcctgcag ggcattcgcg acacggtcga ccgctacgcc cagccgggca
     4921 cggccgaggc cgtcgacgtg cggcccggcg tgctcggtga gcgcgccgag gtgctcggcg
     4981 ccctcgcgct cgtcatccgc gacacggagc ggatcggctc cggcacgctc gtctcgctcg
     5041 cgcgcgaggc cgcgccggcg ctcgccggcg ggcgctagcg aacggagacg acgcgggcgc
     5101 gccaggcgcc cttcggcgcc tcgatctcga cctcgtcgcc gacgtgggcg ccgagcaggg
     5161 cccggccgag cggcgagtcg ggcgagacgc cgccgacgct cgagatctcc acctccatct
     5221 cctcgccgtc gtccgcgcgc gcgatcgtga cgtgcgagcc gaccgcgacc cggccgtcgt
     5281 ccgcgcccgc gccgtcgacg acgaccgcgc ggtggagccg gtcgcggagg atcgtgatcc
     5341 gccgctccag gagcccctgc tcgttcttcg ccgcgtggta ctcgtagttc tccgacaggt
     5401 cgccgaagga gcgcgccgtc ttgatcgcct cgacgatctc ggcgcgcttc gggccctcga
     5461 gctcggccag ctcctcctcg agccgcgtct tctgggcctc ggtgagccgc tctccgtgtt
     5521 ccgtcatccg ccccaggcta cccggccgcc gccgcgagga gcaccacgag cgccgccagg
     5581 acgagcaacg gcgtgacgag cgccgagatc gccgccgccg cgaggaacgg gatgctgagc
     5641 agcggcagga tcggcgcgtg cggggcgagg aggtcgcgcg cggagcgcac gaggccggcg
     5701 agccgccagg tcgaccccgg caggcggcgc cacgccggcg cctgccgcga ctgcgcgtcc
     5761 cgggtgatcg cccgcagctc gccggcgtgc tcgcgggccg tctgcggcga gcgcaggagc
     5821 ttctccggca gctcgaccac ctcgccgagc acgaaggaga agaagagcag gaccccgccg
     5881 ggcacggtga gcagcaggag gaggatgacg gcgccgaccc acgtctcgtc ctcgtccggc
     5941 gtccagcgga ggagcacgac gagcgcgatc accgccgccg cggccagggc gacgagcgcg
     6001 agcttccgcg tccctcgtgc gaccgccgcc gcgaggggga ggatgctgcg cagcacggcg
     6061 aggccgtcgg ctctcatcgg cgtcggcgtt tccggcctcg cgcgaccgca accgccgcgc
     6121 acaggcgtcc ggccgcttcg agctccgcct cgaccggggc gatcacgagc tcctgcccca
     6181 cctcctcgac cccggcgcgg agcgagcgcg aggcggcgcg cgcccgccgc cgggcggcgg
     6241 cgccgttcac gagtcgcgcg aggaaggcga gcgcgaggcc cgcgagcgct ccgccgacca
     6301 gcagccacgt cggcacggga atcccgtacg cctcgggcag cggcacgacg tcctcgacgc
     6361 gcaggtagcc gagcacgagg agaacggcga gccagagggc gcccgcggcg acggcgagcg
     6421 cgaggcccgt ctggaggagc gacaccgcgc gccaccagcg cggccgcgag acgtggaggt
     6481 cggcgcccga gacggcgccg tcgaggcggt cggccacctc ctcgtcacgc gcggtcgccg
     6541 cctcgcgggc gaggcgcggc cagggctccg ggaggccggc ggccgcgcgg tcggcgaggc
     6601 gccgcgccgc ggtcgcaacc tgcgcggtct ggacgtcggt cggccggggc agcgacgtcc
     6661 ggacggcggg ctgcgggcgc tccggcagcc gcagccggcg gagcgggtcg ggccggaagc
     6721 gtcgcaccca gcggacgaac ggccagcccg tgccgagcgc gccgcggcgc cggtgcgcgg
     6781 cctcgaccgc acgcaggacg gccgggacgc ccgccgcgtc ctcgagcgcg gccaggagcc
     6841 gccggcggtc gtcccgctgg acgccggccg ctccgtcctc cccgcacgcg acggcgagcg
     6901 ggagcacggc gtcgtccacg tccgcggcaa gccgggcgat cgccgcgtcg cgggcgcgga
     6961 cgcggtccgc gagcagccgg cggaggtcgt cgagcccggc gccggtctcg gccgacacga
     7021 cgacgaccgg cgcgccgtcg acgccgtcct cggcgagcag ccgctcgagg tctccgcgcc
     7081 accgctccac gtcggccggg gagagcaggt cggcctggtt caggacgacg gccatcgcct
     7141 ccgcgtgcga ggcgagcggg cgcaggtagc ggtcgtgcag ggcggcgtcc gcgtacttct
     7201 gcgggtcgac cacccacgcg acgaggtcgg cgaggccgac gacgcggtcg acctcgaggc
     7261 gatgggcggt ctcgacggag tcgaagtccg gcaggtcgag cagcacgagc ccgtcgagcc
     7321 cgtccggcgc gatccggtgg cgccgcggga tctcgagcca gtcgaggagc ggcccggcgc
     7381 cgtcccccca gacggccgcc tgccccgtgg aggtcgtcgg ccggcggcgc ccgaccgccg
     7441 cgaggtcctc tcccgcgagc gcgttgaaca gccgcgactt gcccgcgccc gtcggcccgg
     7501 cgagcgcgac gaccgtcgac tcgaggccga gcccgagccg ctcgcccgcc cgagccacga
     7561 ccgcgcgcgc ccgctcgacg gcctcgtcgg gaaggcggcc ctcggcgagc ccggacgcct
     7621 cggcgagcgc ctcgagccgg cggtcgaggt cgcccctcac gccgccctgc ggatcgcgtc
     7681 caccgcctcg tgcagccggg cgaacgtgtc cgcctccggc gccgcctcgt cgaggagcgc
     7741 gtcgaaccgc gccgcctccg cgcgcaggag cccgtccacg cgctcgacga ggtcctcgcg
     7801 cgcgcgtgcc gcgagcgtcc ggacggcctg gtcgccgaag atggcctcga gcaccttctg
     7861 gccgacggcc gacgtcccgc ccgcgacgac gacctcggtg ccggtgaggc cgccggtgtg
     7921 cgcgaagacc gcgagcatga cggtcaggcc ggccccgttc acgccgagcg aggcgagccg
     7981 ggcggtcgcg cgcttcgacg cgccctcctc ccggacgagg tcgaagacga cgccctgcca
     8041 cgcgcgcacc tcctcctcgg cggccggctc gagctccgcg gacgcgcggt cgagcccggt
     8101 cgtcccctcg aggagcgccc ggccggggac gcgttcgcgc caggctcgcg cggtgcgctc
     8161 cgcggcccgc tcggcggcgg cgcgcacgac ggcctcgacg cggttctcga cggcctcgcg
     8221 cagctcctcg tcgcccgccg ggcggccggt gacgaggctc ctgaggcggt cgcgcgcctt
     8281 gccgatgccc gtctcgaggg cgcgcatgac gtcgccggtg ccgaccacct cgtgccaccg
     8341 cgcgagcacc tcgccgcgca ggagcgagcc gctgcgcagc gcggcctcga cctcgtggag
     8401 cgcatccacg taggccgcgt cggcctcggc gcggaggtcg gccgcgaccg agatctgctc
     8461 catgacggcg cgctccacga cggcggcgcg cgcggggagg ctcgcgagcg cgccgcgcag
     8521 cgtgcgtcga acgagcgccg cgcgggcctg cgcgtctgcg gcgagatcct cgagccacgc
     8581 gcgcgccggc gcgagcgcgc cctcggggag gagcccgtct gcgagctcgg tctccgggac
     8641 gacgagcagg tcgacctccg cgaggcgctc gtcgcggagc atccggccga ggtgctcggg
     8701 cacctcgcgg ccggcgtcgg ccgggacgcg gttcaggacg agcgagagcg ccgtgccgcg
     8761 gtcgcgcgcg gcgcggagga gctcccaagg cacggcgtcg gcgtagcgcg cggccgtcgt
     8821 gacgaagagc catccgtccg cggcggcgag gagctggttc gcgagcgcgc ggttctccgc
     8881 gagaaccgag tcgatgtccg gcgagtcgag gagcgcgagc cccggaggca gcgccgcggt
     8941 cggcgcgagg gcgagcgtcc cgggcgccgc gtcgcccccg ctcgcccgcg gcaggccggg
     9001 caggacgcgc ggcccctcga accacgcgag gtcgtcggga tggaaggcga gcaccggggc
     9061 gcgcgtcgtc gggcgcagga cgccggccgg gctgatctcc tccccgacga ggctgttcac
     9121 gagcgtcgac ttccccgccc cggtcgatcc gccgacgacg atgagcgcgg gcgcgtccat
     9181 ccggtcgagc cgtggcagga ggtagtcgtc gacctgcgcg acgaggtcgt cgcgcacccg
     9241 ccgggcctcc tccgcgccgg gcacgtcgag gcggaggcgg acggccgcga ggtcgtcgcg
     9301 cagccgcccg agggcggccg gaaggccgtt gctcatgacg agtgaactac ccatcgggag
     9361 ggctccagaa atccttacga acagcgactc gcgtcaggtg ctacattccg cgcccgaacg
     9421 agagcggcgt cggggaggat gccgcgaaac cgaacacagg ggaggaacaa cgatgcacgc
     9481 acgtgcactg cgggccgcgg tgatcgccgc cgccctggcc gtgctcgcgc ttcccgcgac
     9541 ggccggcgca caggggcacg agatgccgcc gtcgggcccg ggcttcctcg agggaccggg
     9601 tgaatggggg aagatcgagc tcgtctcgac cgagttcgtg accgatcagg acgatctcgt
     9661 cgcggacgtc gcgctcgatc cgagccacga ctacgcgtat ctcgcccact ggggcgagcc
     9721 ggactgcgcg gccccgtcgg agggaggcgg caagaacgcg cccgacgccg gagtctgggt
     9781 gatcgacatc acggacctcg agaatcccga ggaggtcggc ttcatcccgt cgtcgcagga
     9841 cagccgtcca ggtgagggga tgcaggcggt cgaggtctcg tcgaagttct tcaccggcac
     9901 gctgctcgcg acgaacaacg agcagtgcgg gccgcagggc aagggcggag tgacgctcta
     9961 tgacgtcacg aacccgctca agccgaagct gctctcgaag cacttcggcg accgcggctt
    10021 cgccgacgct aacgacatcc acaacgtgtt cgtgtgggac gccggcgaga acgtgtacgc
    10081 cgtcatggtc gacaacttcg agacgactga cgtcgacatc ctcgacatca cgaacccgaa
    10141 gcggccgcgg ctcatcgcgg acatcaacct gaacgagtac atcgagggcg gcgtcgacca
    10201 gccggagctc ggcctgaccg actcgttcct ccacgacatg gtcgtgaagc agatcgacgg
    10261 ccgctggatc atgctcctgt cgtactggga cggcggcttc gtgatgctcg acgtgacgga
    10321 tccggcgaac cccgtctaca tcctcgacac cgagttcgcc gagatcgacc cggagctcct
    10381 cgagtcggcg ggcatctcgc tcacgcccga gggcaacgcg caccaggcgg agttcacgat
    10441 cgacaaccgc ttcttcatcg gcggtgacga ggacttcgcg ccctaccgga cggacgagtt
    10501 cgagatcacg agcggcccgt acgcgggcgt ctacccgtcg gtgatcgtcc ccggcggcca
    10561 ggctcccgcg atcctgcccg acctgaccct gaacggtcct gtcgtgtacg gcgggtacgg
    10621 gtgcccgtcc tcggcgccgc ttccgagccc cgaggacatc cccgggtacc tcgacatgct
    10681 cgagccggac gaggaaacga tcatcgtcct ccagcgcggg cccgtcggcg acccgagcgc
    10741 gcccgaggag gcgtgcttcc cgggcgagaa ggcgcacgag gccgctctcg ccggctggga
    10801 cgccgtgctc ttcgtcaacc accacagcgg cgaggccgcg ggcggcgagc cgttctgcgg
    10861 ctccggcgcg ttcgtcgaca acatcgtggc cgtgtgcacg acgcacgccg cgttccaccg
    10921 gctgttcgac ctccagccgc tcgatccgcc ctggacctac ccggagaaca tcgcgatcgg
    10981 ggcgatcggc tcggagatcg aggtcgggtc gctcttcgac ggctggggct acgtgcacct
    11041 ctacgacgcc gagaccggcg ccgagctgga cacgtacgcg atccccgagg cgcacgatcc
    11101 gatgttctcg ctcggcttcg gcgacctcac cgtgcacgag gtggcgacgg atccgcagga
    11161 tgcgagcctc gcgtacctct cgtactactc cggcgggctc atcgccctcc agatccagtg
    11221 cacgaacccg aacgacaact cgacgtgcga gctcgtcgag gtcggcggct accgcgaccc
    11281 gatgggcaac gacttctggg gcgtcgagac gttcgtgggc gaggacggct tcacgtacat
    11341 cctcgcgagc gaccgcgaca gcggcctctg gatcttccgg gatccctgag ccggacctga
    11401 gacgcatgag cgagcgagag gggccggcgc tgccggcccc tctcgtctgc gggaaaggcg
    11461 acggccgccg tcagcggagc tcgtcgagcg cgagctcgtc ggccgcgacg acctcgtaga
    11521 gctcgaggct ccgcatggtg gcgccgaccg cctcggcgac ctcgtcgcga agcgcggcgc
    11581 cgctctgctc gcggttgagg gccgcctcgg cgctcgtcca gaacgtcagc ccgatcgagc
    11641 gcccggcctc tcggtcgagg aggacgatcc agccgcggaa cccttccgcg tcgcgaagcc
    11701 acgggatcac gtcctcgcgg tagacccgca ccccggcctc gatgtcctcg ggtgacccct
    11761 cgaacgtcgt cacgcgcgcg tacacgccgg gaagcttacg cccgctcgcc gcgcggctgg
    11821 ccgtcggcgg ccgcggccgg ctcccagacc cagtcgcgca cgccgggcat gtcgtcgccc
    11881 gtctcgcgcg tgtacgcccg gtggcggagg cgctcgtcca ccatctcctg ccgcaggtgc
    11941 gccgcgcgct cgccgaggcc gggcacgcgg tcgatcacgt ccatcacgag gtggaagcgg
    12001 tcgaggtcgt tcagcatgac catgtcgaac ggcgtcgtcg tcgtcccctc ctccttgtag
    12061 ccccgcacgt ggaggttcgc gtggttcgtg cgccggtagg tgagccggtg gacgagccac
    12121 gggtagccgt ggtaggcgaa gacgatcggg cggctcgtcg tgaagagcgc gtcgaactcc
    12181 gcgtcgggga gcccgtgcgg gtgctcgcgc tcgtcctgca ggcgcatgag gtcgacgacg
    12241 ttcacgaccc gcaccttgag gtcggggagg tgccggcgca ggagcgaggc ggccgcgagc
    12301 gtctcgagcg tcgggatgtc gcccgcgcag gcgagcacga cgtccggctc gccgccctcg
    12361 tccgtcgagg cccagtccca cgtgccgatc ccgcgcgtgc agtgcgcgac cgcctcgtcc
    12421 atcgtgaggt agtcgagcga cggctgcttc cccgccacga tgacgttcac gtagtggcgc
    12481 gacttcaggc agtggtcggc gaccgagagg aggcagttcg cgtcgggcgg caggtagacg
    12541 cggatgatct ccgccttctt gttcacgacg tggtcgatga agccggggtc ctggtgcgag
    12601 aacccgttgt ggtcctggcg ccagacgtgc gacgtgagga ggtagttgag cgacgcgatc
    12661 gggcgccgcc acgggatccc gcgcgtcacc ttcagccact tcgcgtgctg gttgaacatc
    12721 gagtcgatga tgtggatgaa cgcctcgtag cagttgaaga gcccgtgcct gccggtcagg
    12781 aggtagccct cgagccagcc ctggcagagg tgctcgctca ggatctcgag cacgcgcccg
    12841 tcgggcgcga ggttgtcgtc ggtcgtcttg cgctcggcca tccacgcgcg gtcggtcgcc
    12901 tcgaagagcg cggagagccg gttcgacgcg gtctcgtccg ggccgaagac acggaagcgg
    12961 tcctcgttca ggcgcacgac atcgcgcagg tacgtcccga gcacgcgggt cggctcgctc
    13021 gccgtgcgcg cgggcgtctc gacctcgacg gcgtagtcgc ggaagtccgg cagctcgagc
    13081 gggcggagca ggatgccgcc gttcgcgtgc gggttcgcgc ccatgcgccg gtgcccctcc
    13141 ggcgcgagcg cccg
//
LOCUS       DASTFC010000208         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_416394,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000208 DASTFC010000000
VERSION     DASTFC010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416394"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFO88_06245"
     CDS             <1..393
                     /locus_tag="VFO88_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545159.1"
                     /translation="FHASARDAVWEYVMTEDVAAESLVSSPEALTLVGHSHLALEFSL
                     TGPVVRGSLAPEGTELELDEPDRRLLNPGSVGQPRDGDPRAAYLVLDLDARRATFRRV
                     PYPVERTQAEIRERGLPESLAVRLEHGL"
     gene            407..802
                     /locus_tag="VFO88_06250"
     CDS             407..802
                     /locus_tag="VFO88_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545160.1"
                     /translation="MVVVAGCGGAEEERATLPPEVGSELAAAAGDVTAALEAGDPATA
                     QAEAAELLAAVEAALADGDVPRPLAREVRRGAERLAALVDAAQPPPVTPPPVQQGDDD
                     EKDDEVDDDSGPGNGKGKAKGKDKEKDDE"
     gene            799..1890
                     /locus_tag="VFO88_06255"
     CDS             799..1890
                     /locus_tag="VFO88_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9545161.1"
                     /translation="MSDVTTRAFAGGRYVVERELAQGGMATVFLARDRELDRLVAVKV
                     LDAQLAGDEDVAGRFRREALTAARLAHPNVVQVYDAGGEAGEPFIVMEVVQGEPLAAV
                     LAREGMLPPERVRDLGLQASAALGHAHEHGIVHRDVKPANLLLRDDGVLKVTDFGIAR
                     ALDATTQLTQAGTLLGTASYVAPEQARGEAVGPPADVFSLGVVLYEALTGQAPWPIES
                     LAELPAVASTPARPVRELAPGTPPELESAVMHALARNPDYRPADGNALAAELRGGEET
                     PAPTRSEAPTVLFPAPDEEPAKRSSRAGWLVVAAALAVALVAALAALTLGGGEDPAPA
                     PVEPAPALDDPLDQSRSYEQWLRAVTAGG"
     gene            complement(1891..2490)
                     /locus_tag="VFO88_06260"
     CDS             complement(1891..2490)
                     /locus_tag="VFO88_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9545162.1"
                     /translation="MALPQPDFALLRKAQRGDERAFAVIVRSYELPVFNYVLRTVGDR
                     GLAEDLTQDIFLRVYQGLPGFSLRCRFTTWLFQVAKNRLLDELRARERRPYAAVAIED
                     APPIDVVDAPPERIETIDAVWRAVALLNPDLKMSLLLRDVVGLSYHEIADSLEITLAT
                     VKWRIYKAREEVQLALEREGIRLYGRESGVVARDDAVST"
     gene            complement(2529..4022)
                     /gene="gpmI"
                     /locus_tag="VFO88_06265"
     CDS             complement(2529..4022)
                     /gene="gpmI"
                     /locus_tag="VFO88_06265"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET9545163.1"
                     /translation="MTEPYPLVALVVLDGWGCAPPGPGNAVELAETPVFDDLWRRYPH
                     TTLDASGLAVGLPEGQMGTSEVGHLTIGSGRVIHQDLVRVNLAIEDGSYYENEALRGA
                     FARARERGGNVHLLGLVSYGGVHSHIDHLRALWELAAREGMQDRTFVHAFTDGRDVSP
                     HAARDDLAELVREGVRIVTVSGRYYAMDRDKRWERTEEALEAIEGRGEAAPDVETAIR
                     ASYDAGVTDEFVRPVGIAPAWPAPTPDDALVHFNFRPDRARQLSEKLLERGYDLTTMT
                     RYRADFPFPVAFDVQEVAHTMAEVLAAHDARQLHVAETEKYAHVTYFFNGGDEHEWPG
                     ETRILVPSPRDVPTYDEKPEMSAPEVTDRFTAEIGNGYRFAILNYANPDMVGHTGVIP
                     AVTRAVETVDACLGNVVEAVHAAGGVCLVTADHGNAEQLLEADGESPHTAHTTNPVPL
                     VATVDGGALADGGTLADLVPTALDLLGLLPPEEMTGRPLMVGRSARR"
BASE COUNT          540 a         1485 c         1561 g          528 t
ORIGIN      
        1 ttccacgcga gcgcgcggga cgccgtctgg gagtacgtca tgaccgagga cgtggccgcc
       61 gagtcgctcg tcagctcgcc ggaggcgctc acgctcgtcg ggcacagcca tctcgcgctc
      121 gagttctcgc tcaccgggcc cgtcgtccgg ggcagcctcg cgccggaggg gacggagctc
      181 gagctcgacg agcccgaccg gcggctcctg aaccccggct cggtcgggca gccgcgcgac
      241 ggcgacccgc gcgccgcgta cctcgtgctt gacctcgacg cacgcagggc aacctttcgg
      301 cgtgttccgt atccggtcga gcgcacgcag gcggagatcc gcgagcgcgg cctcccggag
      361 agcctcgcgg ttcgcctcga gcacggcctc tagcgccctc ctgctcgtgg tcgtcgtcgc
      421 gggatgcggc ggcgccgagg aggaacgggc gacgcttccg ccagaggtcg gctccgagct
      481 cgccgcagcg gcgggcgacg tgaccgcggc gctcgaggcc ggcgacccgg cgacggcgca
      541 ggcggaggcg gccgagctcc tcgccgcggt cgaggcggcg ctcgcggacg gcgacgtccc
      601 gcgcccgctc gcccgcgagg tacgtcgcgg ggccgagcgg ctcgccgcgc tcgtcgacgc
      661 cgcgcagccg ccgccggtga cgccgccgcc cgtccagcaa ggggacgacg acgagaagga
      721 cgacgaggtc gacgacgact ccggccccgg gaacggcaag ggaaaggcga agggcaagga
      781 caaggagaag gacgacgagt gagcgacgtg acgacacgcg cgttcgccgg cgggcgctac
      841 gtcgtcgagc gggagctcgc gcagggcggg atggcgaccg tcttcctcgc gcgcgaccgc
      901 gagctcgacc ggctggtcgc cgtgaaggtg ctcgacgcgc agctcgcggg ggacgaggac
      961 gtggccggcc gcttccgccg cgaggcgctg accgcggcgc ggctcgcgca tccgaacgtc
     1021 gtccaggtct acgacgcggg cggggaggcg ggcgagccgt tcatcgtcat ggaggtcgtc
     1081 cagggcgagc cgctcgccgc cgtgctcgcc cgcgagggca tgctcccgcc cgagcgcgtc
     1141 cgcgacctcg gcctgcaggc ctccgcggcg ctcggccacg cgcacgagca cgggatcgtc
     1201 caccgggacg tgaagccggc gaacctcctc ctgcgcgacg acggggtgct caaggtgacc
     1261 gacttcggaa tcgcccgcgc gctcgacgcg acgacgcagc tcacgcaggc cgggacgctg
     1321 ctcggaaccg cctcgtacgt cgcgccggag caggcgcgcg gcgaggcggt cggcccgccc
     1381 gcggacgtct tctcgctcgg cgtcgtcctc tacgaggcgc tgaccgggca ggcgccgtgg
     1441 ccgatcgaga gcctcgccga gctgccggcc gtcgcctcga cgcccgcgcg cccggtccgc
     1501 gagctcgcgc ccggcacgcc gccggagctc gagtcggcgg tcatgcacgc gctcgcacgg
     1561 aacccggact accggccggc cgacgggaac gcgctcgcgg cggagctccg cggcggcgag
     1621 gagacgcccg cgccgactcg ctcggaggcg ccgaccgtcc tcttccccgc gcccgacgag
     1681 gagcccgcca agcggtcgtc ccgggccggg tggctcgtcg tcgccgccgc gctcgccgtc
     1741 gcgctcgtcg ccgcgctcgc tgcgctcacg ctcggcggcg gcgaggatcc cgcccccgcg
     1801 cccgtggagc ccgcgcctgc gctcgacgac ccgctcgacc agtcgcggag ctacgagcag
     1861 tggctccgcg ccgtgaccgc gggcgggtag ctacgtagag accgcgtcgt cgcgggcgac
     1921 gacgccgctc tcgcgcccgt agagccggat gccctcccgc tcgagcgcga gctggacctc
     1981 ctcgcgcgcc ttgtagatcc gccacttgac cgtggcgagc gtgatctcga gcgagtccgc
     2041 gatctcgtgg tacgagaggc cgacgacgtc gcgcaggagg agcgacatct tcaggtcggg
     2101 gttcaggagc gcgacggccc gccagacggc gtcgatcgtc tcgatccgct cgggcggggc
     2161 gtcgacgacg tcgatcgggg gcgcgtcctc gatcgcgacg gccgcgtacg gccggcgctc
     2221 gcgggcgcgg agctcgtcga gcagccggtt cttggccacc tggaagagcc aggtcgtgaa
     2281 ccggcagcgc agcgagaagc cggggagccc ctggtagacg cgcaggaaga tgtcctgcgt
     2341 caagtcctcg gcgaggccgc ggtcgcccac cgttcgcagc acgtagttga agaccggcag
     2401 ctcgtaggag cggacgatca ccgcgaaggc gcgctcgtcg ccgcgctgtg ccttgcgcag
     2461 gagagcgaag tcgggttgag ggagagccac agcctgttac cggatggtga accgcagtat
     2521 aggaacggct atcggcgtgc ggaccgccct accattagcg gtcgcccggt catctcctcc
     2581 ggcggaagga gccccagaag gtcgagcgcg gtcgggacga ggtcggcgag cgtgcccccg
     2641 tcggcgagcg ccccgccgtc gacggtggcg acgagcggca ccggattcgt cgtgtgcgcg
     2701 gtgtgcgggc tctctccgtc ggcctcgagg agctgctccg cgttcccgtg gtcggcggtc
     2761 acgaggcaga cgccgcccgc cgcgtggacg gcctcgacga cgttgccgag gcaggcgtcc
     2821 acggtctcga cggcgcgcgt cacggcgggg atgacgcccg tgtgaccaac catgtccggg
     2881 ttcgcgtagt tcaggatcgc gaaccggtag ccgtttccga tctcggccgt gaagcggtcg
     2941 gtcacctccg gcgccgacat ctccggcttc tcgtcgtacg tcggcacgtc gcgcggcgag
     3001 ggcacgagga tgcgcgtctc ccccggccac tcgtgctcgt ccccgccgtt gaagaagtac
     3061 gtcacgtgcg cgtacttctc cgtctcggcg acgtggagct ggcgcgcgtc gtgcgcggcg
     3121 agcacctcgg ccatcgtgtg ggcgacctcc tgcacgtcga acgcgaccgg gaacgggaag
     3181 tcggcgcggt agcgcgtcat cgtcgtcagg tcgtagccgc gctcgaggag cttctccgag
     3241 agctggcgcg cgcggtcggg gcggaagttg aagtggacga gcgcgtcgtc gggcgtcggc
     3301 gcgggccagg cgggcgcgat gccgaccggc cgcacgaact cgtccgtcac cccggcgtcg
     3361 tagctcgcac ggatggcggt ctcgacgtcg ggcgctgcct cgccgcggcc ctcgatcgcc
     3421 tcgagcgcct cctcggtgcg ctcccagcgc ttgtcgcgat ccatcgcgta gtagcggccg
     3481 gagacggtga cgatgcgcac gccctcccgg acgagctccg cgaggtcgtc gcgtgcggcg
     3541 tgcggcgaga cgtcgcgacc gtcggtgaag gcgtggacga acgtgcggtc ctgcatgccc
     3601 tcgcgggcgg ccagctccca gagggcgcgc aggtggtcga tgtgcgagtg gacgccaccg
     3661 tacgagacga ggccgagcag gtggacgttc ccgccccgtt cgcgggcgcg cgcgaacgca
     3721 ccgcgaagcg cctcgttctc gtagtaggag ccgtcctcga tcgcgaggtt gacgcgcacg
     3781 aggtcctggt ggatcacgcg gccggacccg atcgtcaggt ggcccacctc gctggttccc
     3841 atctggccct ccggaagccc gacggcgagc ccggacgcgt cgagcgtcgt gtgcgggtag
     3901 cggcgccaga ggtcgtcgaa gacgggcgtc tcggcgagct cgaccgcgtt gcccgggccc
     3961 ggcggcgcgc agccccatcc gtcgaggacg acgagcgcga cgagcgggta cggctcggtc
     4021 acgcggcggc gacggcttcg gcgcccgcgc ggcagatcgc cgcgaagggc tcgacgtcga
     4081 gcgaggcgcc gccgacgagc gcgccgtcca cgtc
//
LOCUS       DASTFC010000209         5915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1141553,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000209 DASTFC010000000
VERSION     DASTFC010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141553"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VFO88_06270"
     CDS             complement(<1..864)
                     /locus_tag="VFO88_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9545164.1"
                     /translation="MRHRIPADVLLLATVLFWSFNFTVVKYALTNGWEPLSYSSVRFA
                     IGALLFSAFTFGREHTLRVRRDDVWLLLAAAALGIWLNQVSFSYSVRLTTAATVALVF
                     GTLPIFVALISRGFGVERLHARHWLAAGISFAGVGLVAAGGAGGLGGDVGGILLALVA
                     CVTWAAYSVAMGPLMRRYSPYRISAFVLLVGMVPLLLSASRQLVTQDWASLGGLAWAA
                     FFYSLFFSLVFTNVMWFEGIHRVGAARASLYANLQPFLGAFFALVVLSEEMTPLQFAG
                     GLVIGAGIVLAR"
     gene            924..1202
                     /locus_tag="VFO88_06275"
     CDS             924..1202
                     /locus_tag="VFO88_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545165.1"
                     /translation="MAEPAIDDIDYLVGPATPHFAYQIRARVQALVRDLPEDHPVRRY
                     ADEKVALLDRLAHASSKAEEGPVVSDERLGWERLPSSAPAWEPHPPRP"
     gene            1199..1942
                     /locus_tag="VFO88_06280"
     CDS             1199..1942
                     /locus_tag="VFO88_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9545166.1"
                     /translation="MRPFEGKSVLVTGGSRGIGRGIALRFADLGASRVAISYLRNDRA
                     AEETAAELRDRGAEAVTLRGNVGDPDKAAQLVADAGPLDVLVSNAATGVIRPFEELTE
                     KHWDWTLNANARALLTLARHAAPAMRPGSSIVAISSLGGERVLDDYLLVGTSKAALES
                     LVRYLAVELAPKGIRVNAVSAGLVDTGALEFFPDRERMLREFREKTPAGRLTEPDDVA
                     AVVTFLALPEADMIRGQVVVVDGGYAIRA"
     gene            complement(2027..2956)
                     /locus_tag="VFO88_06285"
     CDS             complement(2027..2956)
                     /locus_tag="VFO88_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET9545167.1"
                     /translation="MTLLDRALVQMLPAVPRPVVQRLSSRYIAGPELDDAARVVQELN
                     ARGRMATIDVLGEEVTTPEETLLLVDEYRRVFDTIRDAGLDSNVSVKPTGLGLSIDRD
                     LARENIAEIVRCATERSSFVRIDMEDATTTSDTLEVYRELRGEGLDRVGVVLQARLRR
                     TLDDVEALADLRPNVRLCKGIYLEPPDIAFEDFDEIRSSFVAVLRALLDAGCYVAVAT
                     HDDWLVDRALELLEERGLGPDEYEFQMLLGVREELGDALVRDGHRLRIYVPYGRRWYE
                     YSMRRLQENPRIATYIALDTVNRLIPGKRSVPA"
     gene            complement(2983..3276)
                     /locus_tag="VFO88_06290"
     CDS             complement(2983..3276)
                     /locus_tag="VFO88_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545168.1"
                     /translation="MEGGFGFPFGGDPDDLMRSLREFAEKQTEAMQESQREQFATLTL
                     NTAVELTGAALSQVQLSGTPDEQAIAMRDAMRVLFPEAVALVSAARQGFMRES"
     gene            complement(3291..3839)
                     /locus_tag="VFO88_06295"
     CDS             complement(3291..3839)
                     /locus_tag="VFO88_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545169.1"
                     /translation="MALPEEIDAARRSALGFAEDGEELEGVVPAEPEAGERLYLCAFA
                     RGEERRWLVLDGGGRPVEDRRLVRDAAAIAALCELAEESAGGGDIGELRARLVELRLT
                     ENPEGIEDAEVAAAELQEAILDPPRVASPGYLDAIGLAAQKLERALGGHGSPFAEAMQ
                     VGIGAAEALAAEVERGYKTALA"
     gene            3858..4580
                     /locus_tag="VFO88_06300"
     CDS             3858..4580
                     /locus_tag="VFO88_06300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9545170.1"
                     /translation="MTETLREPNELAQELFAPLAETYDRYAFLLSFGQDARWRRFLVD
                     RVTAGPEDTVCDVACGTAAISLALARRHGCRVVGIDQSAPMLAEGRRRVEAAGLEDRI
                     RLEEGRAEELPFADGELAALTSGYLLRYVEDPGATIRELVRVVRPGGTVAALDFGVPP
                     NPVARAAGRAYVGVGLPFLGGLVSAGWRDVGRVLRDSIPSFYARYPLERQLADWRAAG
                     IHDVQARHPSLGGGVIVWGTRV"
     gene            4573..5304
                     /locus_tag="VFO88_06305"
     CDS             4573..5304
                     /locus_tag="VFO88_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545171.1"
                     /translation="MSEEARPAFYALAPGGVRDYVTLLHPPYTAWHLSYVVIGAAVAG
                     ELEAGRILPTLAAFFLAVGIAAHALDELHGRPLRTEIPARVLVVLAAASLAGAVAIGL
                     YGALTVSPWILLFVAAGAVAVVGYSSELWGGRLHGDLQFALAWGAFPVLTGAFATAER
                     IEAPALLAAAFAFLLSLAQRRLSTQVRDVRRRVARVEGRLERRDGTSEPVTTATLIEA
                     EERALRLLAWAVVVLALALVGLRAA"
     gene            5304..>5915
                     /locus_tag="VFO88_06310"
     CDS             5304..>5915
                     /locus_tag="VFO88_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9545172.1"
                     /translation="MALALAPELPAELQRPALVEPAPNEASFGRIAGTVAPGTHRIVV
                     VVDGVERGAARVKGTRFELRVDLPPRDATVEVRAEDALGNSSLTTIGPVLGLPAGEPP
                     FRPAALDRRLELRLRRLADRFPGIAAIYVENVETGAGAAWNAGARFPAASTVKTAIAL
                     ETLRILAERPPPGQALDLLIRDMLVNSSNYAANELLRWIGGSDT"
BASE COUNT          794 a         2253 c         2113 g          755 t
ORIGIN      
        1 ccgcgcgagc acgatccccg ctccgatcac gagcccgccc gcgaactgga gcggcgtcat
       61 ctcctccgag agcacgacga gcgcgaagaa cgcgccgagg aacggctgga ggttcgcgta
      121 gagcgaggcg cgcgccgcgc cgacccggtg gatgccctca aaccacatga cgttcgtgaa
      181 cacgagcgag aagaagaggc tgtagaagaa cgccgcccag gcgagcccgc cgagcgaggc
      241 ccagtcctgc gtcacgagct gccgcgacgc ggaaagaagc agcggcacca ttccgacgag
      301 caggacgaag gcgctgatcc ggtacggcga gtagcgccgc atgagcggcc ccatggcgac
      361 cgagtacgcc gcccaggtca cgcacgcgac gagggcgagg aggatcccgc cgacgtcccc
      421 gccgagccca cccgcgccgc ccgcggcgac caggcccacg cccgcgaagg agatcccagc
      481 ggcgagccag tggcgcgcgt gcagccgctc gaccccgaag ccccgcgaga tgagcgcgac
      541 gaagatcggc agcgtgccga agacgagcgc cacggtcgcc gccgtcgtga ggcgcaccga
      601 gtacgagaac gacacctggt tcagccagat cccgagagcc gccgccgcga gcaggagcca
      661 gacgtcgtcc cgccgcacgc ggagcgtgtg ctcgcgcccg aacgtgaacg cggagaagag
      721 gagggcgccg atcgcgaagc gcaccgacga gtacgagagc ggctcccacc cgttcgtgag
      781 cgcgtacttg acgacggtga agttgaagct ccagaagagc accgtcgcga gcagaaggac
      841 gtccgcggga atccggtggc gcatggcgcg cggacgatag ccgccgcgcc ccgcccgtcc
      901 cgccgcgcgg tagagtcgcg ccgatggccg agcccgcgat cgacgacatc gactacctcg
      961 tcggccccgc gacgccgcac ttcgcgtacc agatcagggc gcgggtccag gcgctcgtcc
     1021 gcgacctccc ggaagaccat cccgtgcgcc gctacgccga cgagaaggtc gcgctcctcg
     1081 accggctcgc ccacgcctcc tcgaaggcgg aggagggccc ggtcgtcagc gacgagcggc
     1141 tcggctggga gcgcctgccg agctcggccc ccgcgtggga gccgcacccg ccgcgcccgt
     1201 gaggccgttc gagggcaagt ccgtcctcgt gacgggcggc tcgcgcggga tcggcagggg
     1261 gatcgcgctc cgcttcgccg acctcggcgc gtcccgcgtc gcgatcagct acctccgaaa
     1321 cgaccgcgcc gccgaggaga cggccgccga gctgcgtgac cgcggcgccg aggcggtcac
     1381 gctccgcggg aacgtgggcg acccggataa ggccgcgcag ctcgtggcgg acgccggccc
     1441 gctcgacgtc ctcgtctcga acgccgcgac cggcgtcatc cggccgttcg aggagctgac
     1501 cgagaagcac tgggactgga cgctgaacgc gaacgcacgc gcgctcctca cgctcgcccg
     1561 ccacgccgcg ccggcgatgc ggccgggcag ctcgatcgtc gcgatctcga gcctcggcgg
     1621 cgagcgcgtg ctcgacgact acctcctcgt cgggacctcg aaggcggcgc tcgagtcgct
     1681 cgtccgttac ctcgccgtcg agctcgcgcc gaagggaatc cgcgtgaacg cggtctccgc
     1741 cgggctcgtc gacacgggcg cgctcgagtt cttccccgac cgggagcgga tgctgcgcga
     1801 gttccgcgag aagacgcccg ccgggcgcct gaccgagccg gacgacgtcg cggcggtcgt
     1861 gacgttcctc gccctgccgg aggcggacat gatccggggc caggtcgtcg tcgtcgacgg
     1921 cggctacgcg atccgcgcct gaccgaagcg cccaggtcga tgcccgccac gtctcgccgg
     1981 gccactgtcc tgtgcccggc accggacacg gccaccgcgg acacggctac gcgggcacgg
     2041 agcgcttgcc ggggatcagc cggttcactg tgtcgagcgc gatgtaggtc gcgatccgcg
     2101 ggttctcctg gaggcggcgc atcgagtact cgtaccaccg ccgcccgtac gggacgtaga
     2161 tccgcagccg gtgcccgtcg cggacgagcg cgtcgccgag ctcctcgcgg acgccgagga
     2221 gcatctggaa ctcgtactcg tccggcccga gccccctctc ctccaggagc tcgagcgccc
     2281 ggtcgacgag ccagtcgtcg tgcgtcgcga ccgcgacgta gcagcccgcg tcgagcagcg
     2341 cgcggagcac ggcgacgaag ctcgagcgga tctcgtcgaa gtcctcgaag gcgatgtccg
     2401 gcggctcgag gtagatcccc ttgcagagcc ggacgttcgg ccggaggtcg gccagcgcct
     2461 cgacgtcgtc gagcgtccgc cggaggcgcg cctggagaac gacgccgacc cggtcgagcc
     2521 cctcgccgcg cagctcgcgg tagacctcga gcgtgtcgga ggtcgtcgtc gcgtcctcca
     2581 tgtcgatccg gacgaagctc gaccgctcgg tcgcacagcg gacgatctcc gcgatgttct
     2641 cccgggcgag gtcccggtcg atgctcagcc cgagccccgt cggcttcacg ctcacgttcg
     2701 agtcgagccc ggcgtcccgg atcgtgtcga agacccgccg gtactcgtcg acgagcagga
     2761 gcgtctcctc cggcgtcgtc acctcctccc cgagcacgtc gatcgtcgcc atgcggccgc
     2821 gcgcgttcag ctcctgcacg acccgggccg cgtcgtcgag ctccggcccg gcgatgtagc
     2881 gcgacgagag ccgctgcacg acgggccggg gcaccgccgg gagcatctgc acgagcgcgc
     2941 ggtcgagaag ggtcacggcg ggagtctagg tacccgccga gatcagctct cgcgcatgaa
     3001 cccctggcgc gcggcggaca cgagcgcgac cgcctcaggg aagagcacgc gcatcgcgtc
     3061 gcgcatcgcg atcgcctgct cgtccggcgt gcccgagagc tgcacctggg agagcgccgc
     3121 gcccgtcagc tcgaccgccg tgttgagcgt gagcgtcgcg aactgctccc gctgcgactc
     3181 ctgcatcgcc tccgtctgct tctcggcgaa ctcgcggagc gaccgcatga ggtcgtccgg
     3241 gtcgccgccg aacgggaagc cgaatccgcc ttccatcgaa actccttccg ttacgcgagc
     3301 gcggtcttgt agccgcgctc gacctcggcg gcgagcgctt ccgccgcccc gatcccgacc
     3361 tgcatcgcct cggcgaacgg cgagccgtgc ccgccgagcg cccgctcgag cttctgcgcc
     3421 gcgagcccga tcgcgtcaag gtagccgggg ctcgccacgc ggggcggatc gaggatcgcc
     3481 tcctggagct ccgccgccgc gacctccgcg tcctcgatcc cctccgggtt ctccgtcagg
     3541 cgcagctcga cgagccgtgc acggagctcg ccgatgtcgc cgccgcccgc gctctcctcg
     3601 gcgagctcgc agagcgcggc gatcgccgcg gcgtcgcgca cgagccgccg gtcctcgacc
     3661 ggccggccgc cgccgtcgag gacgagccag cgccgttcct cgccgcgcgc gaaggcgcag
     3721 aggtacaggc gctcacccgc ctcgggctcg gcggggacga ccccctccag ctcctccccg
     3781 tcctcggcga acccgagcgc ggagcgccgc gcggcgtcga tctcctccgg gagcgccatc
     3841 cgagagacaa tagccacgtg accgagaccc tccgggagcc gaacgagctc gcgcaggagc
     3901 tcttcgcccc gctcgccgag acgtacgacc gctacgcgtt cctgctctcg ttcggacagg
     3961 acgcacgctg gcgccggttc ctcgtcgacc gcgtcacggc cgggccggag gacaccgtct
     4021 gcgacgtcgc gtgcgggacg gccgcgatct cgctggcgct cgcccgccgg cacgggtgcc
     4081 gcgtcgtcgg gatcgaccag agcgccccga tgctcgccga ggggcggcgg cgtgtcgagg
     4141 cggccgggct cgaggacagg atccggctcg aggagggccg tgccgaggag ctgcccttcg
     4201 ccgacggcga gctcgcggcg ctcacgagcg gctacctgct gcgctacgtc gaggacccgg
     4261 gcgcgacgat ccgcgagctc gtccgcgtcg tgcggccggg cgggaccgtc gcggcgctcg
     4321 acttcggggt gccgccgaac ccggttgcgc gcgccgcggg gcgcgcgtac gtcggcgtcg
     4381 gcctgccctt cctcggcggc ctcgtctcgg ccggctggcg cgacgtcggc cgggtgctcc
     4441 gggacagcat cccgtcgttc tacgcgcgct atccgctcga gcggcagctc gccgactggc
     4501 gcgcggccgg gatccacgac gtgcaggcgc ggcatccgag cctcggaggc ggcgtcatcg
     4561 tctggggcac gcgtgtctga ggaggcgcgt ccggccttct acgccctcgc gccgggcggc
     4621 gtgcgggact acgtcacgct gctccacccg ccgtacaccg cgtggcacct gagctacgtc
     4681 gtgatcggcg ccgccgtcgc aggcgagctc gaggccggac ggatcctccc gaccctcgcg
     4741 gcgttcttcc tcgcggtcgg gatcgccgcc cacgcgctcg acgagctgca cggacggccg
     4801 ctgcggaccg agatccccgc gcgcgtgctc gtcgtcctcg ctgcggcgtc gctcgccggc
     4861 gcggtggcga tcgggctcta cggtgcgctc acggtcagcc cgtggatcct gctcttcgtc
     4921 gccgcgggcg ccgtcgccgt cgtcggctac agctcggagc tctggggcgg tcggctgcac
     4981 ggcgacctgc agttcgcgct cgcgtggggc gcgttccccg ttctgaccgg cgcgttcgcg
     5041 acggccgagc ggatcgaggc ccccgccctg ctcgccgccg cgttcgcgtt cctcctcagc
     5101 ctcgcgcagc gacggctctc gacccaggtg cgcgacgtcc gccgccgcgt cgcacgcgtg
     5161 gagggccggc tggagcgccg ggatgggacg agcgagccgg tcacgaccgc gacgctcatc
     5221 gaggcggagg aacgcgcgct gcgcctcctc gcctgggcgg tcgtcgtcct cgcgctcgcc
     5281 ctcgtcgggc ttcgggcggc ctgatggcgc tcgcgctcgc acccgagctt ccggccgagc
     5341 tccagcggcc ggccctcgtc gagccggcgc cgaacgaggc gtcgttcggc cggatcgccg
     5401 gcacggtcgc gccgggcacg catcggatcg tcgtcgtggt ggacggcgtc gagcgcggcg
     5461 cggcgcgcgt caagggcacg cgcttcgagc tccgcgtcga cctgccgccc cgcgacgcga
     5521 ccgtcgaggt gcgagccgag gacgcgctcg ggaacagctc gctcacgacg atcggccccg
     5581 tgctcggcct gccggccggc gagccgccct tccggccggc cgcgctcgac cgtcgcctcg
     5641 agctgcggct gcgccgcctg gccgaccgct ttcccggcat cgcggcgatc tacgtcgaga
     5701 acgtcgagac gggcgcgggc gcggcgtgga acgcgggcgc gcgcttcccg gcggcgtcga
     5761 cggtgaagac ggcgatcgcg ctcgagaccc tccgcatcct cgccgagcgc ccgccgcccg
     5821 gccaggcgct cgacctcctg atccgcgaca tgctcgtgaa ctcgtccaac tacgcggcga
     5881 acgagctcct gcgctggatc ggcgggtcgg acacg
//
LOCUS       DASTFC010000210         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1108282,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000210 DASTFC010000000
VERSION     DASTFC010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108282"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1514
                     /locus_tag="VFO88_06315"
     CDS             102..1514
                     /locus_tag="VFO88_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9545173.1"
                     /translation="MPDAVVVGSGPNGLTAAIVLARAGRSVVVREALDVVGGGVHSAE
                     LTLPGFVHDTCSSIYPLGAGSPVFRRLPLAEHGLEWAHSDAALAHPLDGGRAAILERD
                     LDATAAGLGEDEAAYRALFGPLVRDWPALERSILRPLVRVPRHPVALARFGLLALRSA
                     EGLARARFRGEKARALFAGCAAHSTLRLDRSPSASFGLVLMALGHVAGWPVARGGAQR
                     LADALASYLRSLGGEIETGARVERLAELDGAGAVLADVSPRELLRLGGEGLPLRYRRA
                     LERYRYGPGSFKLDLALDGPIPWEAPEVGRAACVHVGGTLDEVAAAERAPWEGRPAER
                     PFVLLAQQSVADPSRAPDGKHTVWAYCHVPNGSTFDMTERIERQIERFAPGFRKRILA
                     RAVTTPADFERRNPSLAGGDINAGAQDLPQLLFRPVPRLVPYTTPVPDLFLCSASTPP
                     GGGVHGLCGFGGAQAVLAAR"
     gene            1761..>2408
                     /locus_tag="VFO88_06320"
     CDS             1761..>2408
                     /locus_tag="VFO88_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9545174.1"
                     /translation="MEAPAPFSPAVRDWFARTFDAPTPAQERGWPAISSGAHTLIQAP
                     TGSGKTLAAFLWALDRAEPGKGTQVLYVSPLKALNYDVERNLRGPLAGIGSALSVGVR
                     TGDTPPKERAQMQRRPPDILITTPESRFLLLTSQAREILRTVRFFILDEAHAVAGTKR
                     GAHLALSMERLDALLPEPMQRIGLSATQRPMEEIGRFVSGGRAVELVDAGRTKPLD"
BASE COUNT          263 a          946 c          876 g          323 t
ORIGIN      
        1 gacgcgaagg gagaccggat cctgctcgcg cggccggggc cgacgctcga ctactgggag
       61 ggcgaggact aggaggccgg cctccgggca agagtgcgtc catgccggac gccgtcgtcg
      121 tcgggagcgg gcccaacggt ctcacggccg cgatcgtgct cgcgcgggcc gggcggtcgg
      181 tggtcgtgcg cgaggcgctc gacgtcgtcg gcggcggggt gcactccgcc gagctgacgc
      241 tgccgggctt cgtccacgac acctgctcgt cgatctaccc gctcggcgcc gggtcgccgg
      301 tcttccggcg tctgccgctc gccgagcacg ggctcgagtg ggcgcactcg gacgccgcgc
      361 tcgcgcaccc gctcgacggc ggacgggcgg cgatcctcga gcgcgacctc gacgcgaccg
      421 ccgcgggact cggcgaggac gaggccgcct accgcgcgct cttcggcccg ctcgtccgcg
      481 actggccggc gctcgagcgc tcgatcctgc gcccgctcgt ccgcgtgccg cgccacccgg
      541 tcgcgctcgc ccggttcggc ctcctcgcgc tccgctcggc ggagggcctc gcccgggccc
      601 gcttccgcgg ggagaaggcc cgcgcgctct tcgccggctg cgcggcgcac tcgacgctcc
      661 ggctcgaccg ctcgccgtcc gcctccttcg ggctcgtcct catggccctc gggcacgtcg
      721 ccggctggcc ggtcgcccgc ggcggggcgc agcggctcgc ggacgcgctc gcctcgtacc
      781 tgcgctcgct cggcggcgag atcgagaccg gcgcgcgcgt cgagcgcctc gcggagctcg
      841 acggcgcggg cgccgtgctc gccgacgtct cgccgcgcga gctcctacgg ctcggcggcg
      901 aggggctccc gctgcgctac cgccgcgcgc tcgagcgcta tcgctacggg ccgggctcct
      961 tcaagctcga tctcgcgctc gacgggccga tcccgtggga ggcgcccgag gtcggccgcg
     1021 ccgcgtgcgt ccacgtcggc gggacgctcg acgaggtcgc ggccgccgag cgcgccccct
     1081 gggagggccg gccggccgag cggccgttcg tcctcctcgc ccagcagagc gtcgcggacc
     1141 cgtcccgcgc cccggacggg aagcacaccg tctgggcgta ctgccacgtg ccgaacggct
     1201 cgacgttcga catgacggag cggatcgagc gccagatcga gcggttcgcg cccgggttcc
     1261 ggaagcggat cctcgcgcgg gcggtcacga cgcccgcgga cttcgagcgc cggaacccga
     1321 gcctcgccgg cggggacatc aacgcggggg cgcaggatct cccgcagctc ctcttccggc
     1381 cggtgccccg gctcgtcccg tacacgacgc ccgtgccgga cctcttcctc tgctcggcct
     1441 cgacgccccc gggcggcggc gtgcacgggc tctgcggatt cggtggcgct caggccgttc
     1501 tcgccgcgcg ttgaggaata tcaacgcacg aaccgttgcg cgaacctagt ggttttcgcc
     1561 ttcggcgcta tcgtttcgcc aacgcaccga gcccctcgcc tctcctggtg gggcgcgcgg
     1621 ctcggccccc gcatcggcgc tcctgcgcca cccgcgggga agccgagtga ctggatggat
     1681 ccaggtccct cccggaaccg accagtcgct cggcttgcgg ctgcctgcgt ggccccggcg
     1741 cccgaggcta cgctggccgg atggaggcgc cggccccgtt cagccccgcc gtgcgcgact
     1801 ggttcgcgcg caccttcgac gcgccgaccc cggcgcagga gcgcggctgg cccgcgatct
     1861 cgtccggcgc gcacacgctg atccaggcgc cgaccggctc gggaaaaacg ctcgccgcct
     1921 tcctctgggc gctcgaccgc gcggagcccg gcaaggggac gcaggtgctc tacgtctcgc
     1981 cgctcaaggc cctcaactac gacgtcgagc ggaacctccg cgggccgctc gccgggatcg
     2041 gctcggcgct ctcggtcggc gtgcgcacgg gcgacacgcc gccgaaggag cgcgcgcaga
     2101 tgcagcggcg cccgccggac atcctcatca cgacgccgga gtcgcgcttc ctcctcctca
     2161 cctcgcaggc gcgggagatc ctccgcaccg tccgcttctt catcctcgac gaggcgcacg
     2221 cggtcgcggg gacgaagcgc ggcgcgcacc tcgcgctctc gatggaacgg ctcgacgcgc
     2281 tgctccccga gccgatgcag cggatcgggc tctcggcgac gcagcggccg atggaggaga
     2341 tcgggcggtt cgtctccggc ggacgtgcgg tcgagctcgt cgacgcgggg cggacgaagc
     2401 cgctcgac
//
LOCUS       DASTFC010000211         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_4353,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000211 DASTFC010000000
VERSION     DASTFC010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4353"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFO88_06325"
     CDS             complement(<1..366)
                     /locus_tag="VFO88_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9545175.1"
                     /translation="MRIALAASFAALACAALAGPAATALQLVTVESGFTGPVHVAATK
                     AEPNRVYVVEQRGVIKIVQNGTTLSQPFLDIRSRVACCGEQGLLSLAFHPRYPQVRRV
                     YVNFTNSGGDTRVVEYRVNE"
     gene            complement(424..1038)
                     /locus_tag="VFO88_06330"
     CDS             complement(424..1038)
                     /locus_tag="VFO88_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545176.1"
                     /translation="MRSAALSRGAGGRALRLATAVVAAGLLMGLAVWSQAARATIFDK
                     GFYEFPYTFSYDDCGFPVEVEGVASGHFRLRTGTGEQASTFFLRDTFSYREVHTNLET
                     GLSFVVRGHGVFNEVRATPLGGTLFAFEAVEAGQPFVIEDAEGNVVVRDRGALRHRIV
                     FDTLGDDVPGGIVIEEFESSVHGPHPGFAEDFPFCEIAAELTGA"
     gene            complement(1145..2491)
                     /locus_tag="VFO88_06335"
     CDS             complement(1145..2491)
                     /locus_tag="VFO88_06335"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9545177.1"
                     /translation="MPGYDPQEGDRISPVPPVGEPRGVRRLLRLAAMDLAPLRRRRDF
                     RLLFVGQGLSFFGSMLTYVAIPYQAYDLTGSTLVLGLLAAVELPPILLAAFVGGALAD
                     AVDRRRLVQLAELGLAACTALLLVNALLPEPRLWPLFVLGAAMAGLVGIQRPPLDAME
                     PRLVPPEELPAASALGSFRSNLGMVAGPPVAGVLIATVGLPATFAVDLVTFAASLVVL
                     NLMRAMPPPPEGARPSLRGIVEGVRYARSRPELLGTYGVDFVAMFFGMPMALYPAFAA
                     DFGGPEVLGLLYAAPSAGALVATLTSGWTGRVHRHGMAVIVAALGWGVGITALGLAPS
                     LWLACLALGFAGACDMVSGIFRSVIWNQTIPDHLRGRLAGIEQVSYSSGPLLGDLEAG
                     VVAAIAGVRASIVSGGVLCVAGVAVLALALPAFRRYDARAHGRIGGTRATTPAAPA"
     gene            complement(2658..3089)
                     /locus_tag="VFO88_06340"
     CDS             complement(2658..3089)
                     /locus_tag="VFO88_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545178.1"
                     /translation="MWPRRLVVVSIASLVFWAVATLVAVQAGWPAELGELGDPNDVSG
                     EWASRGTLVSPPLLPLVLQALFTLLVFVDRRITVGIAGLALAALGAVYVVGGLGEPLE
                     PARSDPPVALYWALKALGTALGLGLVVTGVETVSAAVRDRL"
     gene            3165..>3820
                     /gene="heR"
                     /locus_tag="VFO88_06345"
     CDS             3165..>3820
                     /gene="heR"
                     /locus_tag="VFO88_06345"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HET9545179.1"
                     /translation="MDTDSPARRLRVWNAALAVLHAAQGALVLVLANDFALPVTIGFL
                     QGPPGSELASETLFELPIGPAVAAFLFLAALDHAIVAAPGGAAWYERNLRRERNPARW
                     YEYAFSASLMVVLIAMLTGIADVAALIALFGVNAAMILFGLAMEEANEPGRPVTWRPF
                     VYGSIVGAIPWLAIGLQLGYSEAETGDVPTFVYAIFFSLFVLFFSFAVNMALQYGRVG
                     P"
BASE COUNT          575 a         1422 c         1380 g          443 t
ORIGIN      
        1 ctcgttcacg cggtactcga cgacgcgcgt gtccccgccc gagttcgtga agttcacgta
       61 gacgcgccgc acctgcgggt agcgcgggtg gaaggcgagc gagaggaggc cctgctcgcc
      121 acagcaggcg acgcgcgagc gaatgtcgag gaacggctgc gagagcgtcg tcccgttctg
      181 gacgatcttg atcaccccgc gctgctcgac gacgtagacc cggttcggct ccgccttcgt
      241 cgcggcgacg tgcaccgggc ccgtgaagcc cgactcgacc gtgacgagct ggagcgcggt
      301 ggcggcggga ccggcgagcg ccgcgcaggc gagcgcggcg aaggaggcgg cgagggcgat
      361 gcgcaccgcc ggtcagggta gcgcgcccgc gtgccgcctc ctccgccggc cgtccggccg
      421 ggctcaggcc cccgtcagct ccgccgcgat ctcgcagaac gggaagtctt ccgcgaaccc
      481 gggatgcggg ccgtggacgg acgactcgaa ctcctcgatc acgatcccgc cgggaacgtc
      541 gtcgccgagc gtgtcgaaga cgatccggtg ccgaagcgcg ccccggtcac gcacgaccac
      601 gttgccctcc gcgtcctcga tcacgaacgg ctggcccgcc tcgaccgcct cgaaggcgaa
      661 gagcgtgccg ccgagcggcg tcgcccgcac ctcgttgaag acgccgtgcc cgcgcacgac
      721 gaacgacagg ccggtctcga ggttcgtgtg cacctcccgg tacgagaacg tgtcccggag
      781 gaagaacgtc gaggcctgtt cgcccgtccc cgtgcgcagg cggaagtggc cgctcgccac
      841 gccctcgacc tcgaccggga agccgcagtc gtcgtaggag aaggtgtacg ggaactcgta
      901 gaagcctttg tcgaagatcg tggcccgcgc agcctgggac cagaccgcca ggcccatcag
      961 gaggcccgcc gcgaccaccg ccgtcgccag gcgaagcgct cgtcctcccg ccccccgaga
     1021 gagtgccgcc gaccgcatga cagctccttt ccgttgaccg caagaggtgg ggcgggactc
     1081 aagcacccgc accaccttgt gtcaagagaa gcgatctcgc ttcggcccgc ccgccccgcc
     1141 cccgctacgc cggggcggcc ggggtcgtgg ccctcgtccc cccgatccgg ccgtgggcgc
     1201 gcgcgtcgta gcgccggaac gcgggcagcg cgagcgcgag caccgccacg ccggcgacgc
     1261 acagcacccc gccggacacg atcgacgccc gcacgcccgc gatcgccgcg acgacgcccg
     1321 cctcgaggtc gccgagcagc ggccccgacg agtagctcac ctgctcgatc cccgcgaggc
     1381 ggccgcgcag gtggtcgggg atcgtctggt tccagatcac ggagcggaag atcccgctca
     1441 ccatgtcgca cgcgccggcg aagccgagcg cgagacacgc gagccagagg gacggggcca
     1501 ggccgagcgc ggtgattccg acgccccagc cgagcgccgc gacgatcact gccatcccgt
     1561 gccggtgcac gcgccccgtc cacccgctcg tgagcgtggc gacgagcgcc ccagcggacg
     1621 gcgccgcgta gaggagccct agcacctcgg gcccgccgaa atcggcggcg aacgccgggt
     1681 agagcgccat cggcatcccg aagaacatcg ccacgaagtc cacgccgtac gtgccgagga
     1741 gctccgggcg cgagcgcgcg taccggacgc cctcgacgat cccgcgcagg ctcggccggg
     1801 cgccctcggg cggcggcggc atcgcgcgca tgaggttcag gacgacgagc gaggccgcga
     1861 aggtgacgag gtcgaccgcg aacgtcgcgg gcagcccgac ggtggcgatg agcacgcccg
     1921 cgaccggcgg cccggcgacc atgccgaggt tggagcggaa ggacccgagc gcgctcgccg
     1981 ccgggagctc ctcgggcggg acgagtcgcg gctccatcgc gtcgagcggc ggccgctgga
     2041 tgcccacgag ccccgccatc gccgcgccga gcacgaagag cggccacagc cggggctccg
     2101 gcaggagcgc gttgacgagc aggagcgcgg tgcaggccgc gagcccgagc tcggcgagct
     2161 ggacgagccg gcgccggtcg accgcgtccg cgagcgcgcc gccgacgaac gcggccagga
     2221 ggatgggcgg cagctccacg gccgcgagca ggccgaggac gagcgtcgag cccgtcaggt
     2281 cgtatgcctg gtacgggatc gcgacgtacg tgagcatcga gccgaagaac gagagcccct
     2341 gccccacgaa gagcagccgg aagtcgcggc gccgccgcag cggggcgagg tccatcgcgg
     2401 ccagccggag gagccgacgc accccgcggg gctccccgac cggcgggacg ggggagatcc
     2461 ggtcgccttc ctgcgggtcg tagcccggca cgcgcgggag tatggcgccg gagccgcata
     2521 aagaaggggc ggttcccaga ccgcccctcc ccttctttgc cccccatgct ccaggaggtc
     2581 ttgcccgacc ggccgccggg cgatcggcag gtcaccgcca gtgaagcacc aaccgggcgg
     2641 ggaatcaacc cccgctgcta cagcctgtcg cggacggcgg cggagaccgt ctcgacgccc
     2701 gtcacgacga gcccgagccc gagcgcggtg ccgagcgcct tgagtgccca gtagagcgcg
     2761 accggtgggt ccgagcgggc cggctcgagc ggctccccga gcccgccgac gacgtagacc
     2821 gccccgagcg cggcgagcgc gagccccgcg atcccgacgg tgatccgcct gtcgacgaag
     2881 acgagcaggg tgaagagcgc ctggaggacg agcgggagga gcggcggcga gacgagcgtc
     2941 ccacgcgagg cccactcgcc gctcacgtcg ttcgggtcgc cgagctcgcc gagctcggcc
     3001 ggccagccgg cctgcaccgc cacgagcgtc gcgaccgccc agaagacgag cgaggcgatc
     3061 gagacgacga cgaggcggcg gggccacacg acggcgcgat cgtacttcgc gccgccgcac
     3121 ggatccggcg ttcgtcctgc ggcgtctggg gtacgagacg gaggatggac accgactccc
     3181 cggctcggcg cctccgcgtc tggaacgcgg ccctcgccgt cctgcacgcg gcgcagggcg
     3241 ccctcgtcct cgtgctcgcg aacgacttcg cgctcccggt gacgatcggc ttcctccagg
     3301 gcccgcccgg aagcgagctc gcgtccgaga ccctcttcga gctcccgatc gggccggccg
     3361 tcgcggcgtt cctcttcctc gcggccctcg accacgcgat cgtcgcggcg cccggcggcg
     3421 cggcgtggta cgagcggaac ctccggcgcg agcggaaccc ggcgcgctgg tacgagtacg
     3481 ccttcagcgc ctcgctcatg gtcgtcctga tcgcgatgct gacggggatc gccgacgtcg
     3541 cagcgctcat cgccctcttc ggggtgaacg cggcgatgat cctcttcggg ctcgcgatgg
     3601 aggaggcgaa cgagcccggc cgcccggtga cgtggcggcc cttcgtctac ggctcgatcg
     3661 tcggcgcgat cccgtggctc gcgatcgggc tccagctcgg gtactcggag gcggagaccg
     3721 gcgacgtgcc gaccttcgtg tacgcgatct tcttctccct cttcgtcctc ttcttcagct
     3781 tcgcggtcaa catggcgctc cagtacgggc gcgtcgggcc
//
LOCUS       DASTFC010000212         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_416584,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000212 DASTFC010000000
VERSION     DASTFC010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416584"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..852)
                     /locus_tag="VFO88_06350"
     CDS             complement(121..852)
                     /locus_tag="VFO88_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9545180.1"
                     /translation="MPMSRKPGAKSSTAAAVHETLAAVLQVRDGGLQVLLWERARKPE
                     AGAWALPGGVLDPAETLEESIRRHLAAKVDVRELSHLEQLQTLSEPDRHPGGRVLATA
                     YLGLVPAGIDPALPEDTAWHPVDELPRTAFDHGAVALAGRERLRAKLSYTNLGFALAP
                     PTFTISELRSLYRAALGHDVSATNLQRVLLRRRLLVETGERRGPGRTGGRPAAVFRFR
                     TQDLEVTDQFAVLRPPGRASAGGGG"
     gene            875..1423
                     /locus_tag="VFO88_06355"
     CDS             875..1423
                     /locus_tag="VFO88_06355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET9545181.1"
                     /translation="MKALIIVDMLDDFVTGSLANPRAQRIVEPLQRLLGHAREEGWVV
                     VFSNDAHEADDPELKVWGEHAMKGTPGAQVIKELEPKPGEREIISDKRGYGAFDFTGL
                     DEQLKALGVDEVVITGQHTHICVRHSSYGALIRGYEITIPRDAVCAFEGVDEDQALEY
                     LEGTYAARITTVDELVGAPVAA"
     gene            1490..1909
                     /locus_tag="VFO88_06360"
     CDS             1490..1909
                     /locus_tag="VFO88_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545182.1"
                     /translation="MPAVPVRTAAIAACGVSGGIHAALFVLHAGELPLHAAAFLTAAL
                     LLALAGVVLARRPAVREAPLGTAALFAALVLAYPLAAHEPFDAIAGIALAVEGIGLAA
                     SLALLRPRRAVVEPGILSIFLLCVALGLMLGGGHGHG"
     gene            1919..>2431
                     /locus_tag="VFO88_06365"
     CDS             1919..>2431
                     /locus_tag="VFO88_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9545183.1"
                     /translation="MNRLADETSPYLLQHADNPVDWYPWGEEALARARGEGKPILLSV
                     GYAACHWCHVMEHESFEDEETAALMNERFVCVKVDREERPDVDGLYMEAVVALTGHGG
                     WPLTVFLTPDGRPFWGGTYFPPEPRHGLPSFRQVLAAIAEAFDGRRGDLDRQADVLTE
                     AIRDASARAPS"
BASE COUNT          333 a          880 c          896 g          322 t
ORIGIN      
        1 agctcgaggt cggagccggg cggatccttg accgcccacc agatcaccca cgcgaggtag
       61 agaacgggaa gcttcagcac cacgaggaac cagacggcgg tccacacgac ggcgcggagt
      121 ctacccgcct ccgccggcgg aagcccgtcc cggcggccgc aggacggcga actggtcggt
      181 cacctcgagg tcctgcgtcc ggaagcggaa gaccgccgct ggccgcccgc ccgtccgccc
      241 cggcccgcgg cgctcgccgg tctccacgag cagccggcga cggaggagga cgcgctggag
      301 gttcgtcgcc gacacgtcgt ggccgagcgc cgcccggtag agcgaccgca gctcggagat
      361 cgtgaacgtg ggcggcgcga gcgcgaaccc gaggttcgtg tacgagagct tcgcccggag
      421 ccgctcccgc ccggcgagcg cgaccgcgcc gtggtcgaac gccgtccggg gcagctcgtc
      481 gaccgggtgc cacgcggtgt cctcggggag cgccgggtcg atcccggccg ggacgagacc
      541 gaggtacgcg gtcgcgagca cacgcccgcc gggatggcga tccggctcgc tcagcgtctg
      601 gagctgctcg aggtgcgaca gctcccgcac gtcgaccttc gccgcgagat ggcgccggat
      661 ggactcctcg agcgtctcgg ccgggtcgag cacgccgccg gggagtgccc acgcccccgc
      721 ctccggcttg cgtgcgcgct cccagaggag cacctggagc ccgccgtcac gcacctggag
      781 gacggccgcg agcgtttcgt ggacggcggc ggctgtgcta gacttcgcgc caggttttcg
      841 actcataggc ataaactagc ggaaggatac ggacatgaag gctctcatca tcgtggacat
      901 gctcgacgac ttcgtgaccg gctcgctcgc caacccgcgc gcgcagcgga tcgtcgagcc
      961 cctccagcgt ctgctcgggc atgcgcgcga ggagggctgg gtcgtcgtct tctcgaacga
     1021 cgcgcacgag gcggacgacc cggagctcaa ggtgtggggc gagcacgcca tgaagggcac
     1081 gcccggcgcc caggtcatca aggagctcga gccgaagccc ggcgagcggg agatcatctc
     1141 cgacaagcgg ggctacggcg cgttcgactt caccggcctc gacgagcagc tcaaggcgct
     1201 cggcgtggac gaggtcgtca tcaccggcca gcacacccac atctgcgttc ggcactcctc
     1261 gtacggcgcg ctcatccgcg ggtacgagat cacgattccg cgcgacgcgg tctgcgcctt
     1321 cgagggcgtg gacgaggacc aggcgctcga gtacctcgag ggcacgtacg cggcgcgcat
     1381 caccaccgtc gacgagctcg tcggcgcacc ggtcgcggcg taggcgggag acagccgcgc
     1441 gacggcctgc ccgcttgggg atcctccccg cgggtaggcc gtcggcgtgg tgcctgcggt
     1501 tccggtgagg acggccgcga tcgcggcctg cggggtgagc ggcggcatcc acgccgcgct
     1561 cttcgtgctg cacgcgggtg agctcccgct ccatgccgcg gccttcttaa ccgcggcgct
     1621 cctgctcgcg ctggcggggg tggtgctcgc acgcaggccg gccgtgcggg aggcgccgct
     1681 cgggacggct gcgctcttcg cagcgctcgt cctcgcgtac ccgctcgccg cgcacgagcc
     1741 gttcgacgcg atcgcgggga tcgcgctcgc cgtggagggc atcggcctcg cggcgagcct
     1801 cgcgctcctg cgcccgcgcc gcgcggtcgt cgagccgggc atcctctcga tcttcctcct
     1861 ctgcgtcgcg ctcggcctca tgctcggcgg cgggcacggc cacgggtagc gtggccccgt
     1921 gaaccgcctc gccgacgaga cgagcccgta cctcctccag cacgcggaca accccgtcga
     1981 ctggtacccg tggggcgagg aggcgctcgc gcgggcccgc ggggaaggga agccgatcct
     2041 cctctcggtc ggctacgcgg cgtgccactg gtgccacgtg atggagcacg agtcgttcga
     2101 ggacgaggag acggccgcgc tcatgaacga gcgcttcgtc tgcgtgaagg tcgaccgcga
     2161 ggagcggccg gacgtcgacg ggctctacat ggaggcggtc gtcgccctga ccgggcacgg
     2221 cggctggccg ctcaccgtct tcctcacgcc ggacgggcgg ccgttctggg gcgggacgta
     2281 cttcccgccc gagccgcgcc acgggctgcc gagcttccgg caggtcctgg cggcgatcgc
     2341 cgaggcgttc gacggccggc gcggcgacct cgaccggcag gcggacgtcc tcacggaggc
     2401 gatccgcgac gcgagcgcgc gggcgccgtc c
//
LOCUS       DASTFC010000213         5849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1438461,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000213 DASTFC010000000
VERSION     DASTFC010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438461"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VFO88_06370"
     CDS             complement(<1..1458)
                     /locus_tag="VFO88_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET9545184.1"
                     /translation="MGLHDRIKSGSNGTGANGGGANGAVAEPHAALAAATPRESAPET
                     ALDPYAELKTRIHHACIAKLGPQLFSTEAAGDLSDQVMRTVTEQLALDRTPLTREERR
                     RITREIADDILGYGPLEPFLRDDSITEVMVNNWDKIFVERGGKLERTNASFADNAHLL
                     RIIDKIVSQVGRRVDESSPMVDARLPDGSRVNAIIPPLALRGPTLTIRKFSRDPFTMD
                     DLIEFGSLTPKAAHFLSACVRGKLNVLISGGTGTGKTTTLNALSAYVPGNERIVTIED
                     AAELQLQQEHVITLESRPANIEGKGEVRIRELVRNALRMRPDRIIVGEVRGPETLDML
                     QAMNTGHEGSLTTIHANSPRDALSRVETLVLTAGVDLPLRAIREQIASAFDLLVQITR
                     LVDGSRKITFVSEVLGMESDVITLQDIFIARPPDEDHAGSGQATKLLGPLLCTGLKPH
                     FLEKMAANGVSIPANFFFPDESGGESFTVASYGGFS"
     gene            complement(1459..2652)
                     /locus_tag="VFO88_06375"
     CDS             complement(1459..2652)
                     /locus_tag="VFO88_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545185.1"
                     /translation="MNLETQTTGTREKTRIFMTGDCEGLQELRHALERHHEVEFVGAA
                     ASLGEAAPALRGGHLGVVLHGTRSSTLPQGELAAIREHTQAPVILLASGEASNLLEEA
                     LEADVADVLLLPQMTENLIFAIRKAGHSGRRLQGGGGRRGRIVTVFSPKGGTGKTVLA
                     SNTAASVAKDEGKKALLLDLDLQFGDAAIMLGIEPEKTIYDLVVAPGELDTEKLAGYV
                     TRHASGLDILPAPLRPEDAELVTEAKLAQLLEVARESYDVIVVDTSPFFHGPMLATLD
                     RTDELLLICSLDVPTLKNVRLALQTLELLSFPTDRISLVLNRANSKVGMKPGEVESAL
                     EVKVRFSVPSDRAVPIAVNRGNPAVLADSRADFSKAVREISKSVVPLDKGDGKTGRRL
                     FRKAA"
     gene            complement(2654..3433)
                     /gene="cpaB"
                     /locus_tag="VFO88_06380"
     CDS             complement(2654..3433)
                     /gene="cpaB"
                     /locus_tag="VFO88_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9545186.1"
                     /translation="MTYRLRNIGIAIALALVAGLLTVFYVTNYKKEVRNAEANVTVFV
                     ASQDIPAGTSGAEAQGFLTEQEVAKRNVTPGAISAPEQLDGRVASATIYAGEQVTTRR
                     FTTEEAKGIRAQLEGNERALSVPGTAEQLLAGTLVAGDRVDVVGSWNVPESETNHYSR
                     VVLRDLLVLEAPEGGVTTESVTNPQAASGGYSTILALTDAQAQKLFWLTQNGTWSLQL
                     RPATDAADSPESAESAESLLLDGIGRARFQAMSARLLEGGE"
     gene            complement(3461..4390)
                     /locus_tag="VFO88_06385"
     CDS             complement(3461..4390)
                     /locus_tag="VFO88_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET9545187.1"
                     /translation="MTRARRQTGQATVTTVLFLTVLLGMTGAVLDVGVWFNEDRKLQA
                     TTDAAALAAAQELPDSTALATTRAIEYGDKNGGGVGAEDITYETGVLANDTIVVQTEK
                     DAPGIFTKLFGIDSVEVHATAKARTGVLSAARWVAPIVVNWQHPMLQCTPPPCTSPTQ
                     IDLMNLHSPGGGDAAGAFGLINLNRNDPNGNVGASTLADWMETGFEGEMGLGNYWQAP
                     STNFNNGQFQSALGSRLNTEVLFPIYRTISGPGSNARYDIIGWVGFVPTSFHAGGSTG
                     RVNGRFVRVMWEGIASSSPSQPSFGAFTVSLVE"
     gene            complement(4387..4746)
                     /locus_tag="VFO88_06390"
     CDS             complement(4387..4746)
                     /locus_tag="VFO88_06390"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HET9545188.1"
                     /translation="MTEFALVLPVIALLLFAIIQCGIAFNNYLTVTDAARAAARKGAT
                     AMYGGGSPSSAATTAARASAQNLDQSKFQVAVTASPRWEKGADVIVTTSYPYEISLLG
                     FVVKSGNVSSTTRERIE"
     gene            complement(4901..5203)
                     /locus_tag="VFO88_06395"
     CDS             complement(4901..5203)
                     /locus_tag="VFO88_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545189.1"
                     /translation="MTTHVIRRNEGLSPTTTARARRSTFIGLTSSIRAARVRRREVNT
                     LTSFFSYLNTLRKGEEGQTMAEYGVVLAVIAIGVVAALLTLSGAISDAINRVVGFL"
     gene            5463..>5849
                     /locus_tag="VFO88_06400"
     CDS             5463..>5849
                     /locus_tag="VFO88_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545190.1"
                     /translation="MPQPRSSSRTPLARLVFFLVLSWALVALGNRKGEIAEHAVPERA
                     EPVGKIPARARRRSRRQRLATSLAFTTLFFAGAALSAGAGDMLVEAMDTTGTDTTETT
                     ETTPAEDAAADGETPADDATAGETPTE"
BASE COUNT          827 a         1990 c         2082 g          950 t
ORIGIN      
        1 cgagaacccg ccgtagctcg cgaccgtgaa cgactcgccc ccgctctcgt ccgggaagaa
       61 gaagttcgcc gggatcgaga cgccgttcgc cgccatcttc tcgaggaagt gcggcttcag
      121 gccggtgcag aggagcggcc cgaggagctt cgtcgcctgg ccggagccgg cgtggtcctc
      181 gtccggcggg cgtgcgatga agatgtcctg gagcgtgatc acgtccgact ccatgccgag
      241 cacctcggag acgaacgtga tcttgcgcga gccgtcgacg agcctcgtga tctggacgag
      301 caggtcgaac gcgctcgcga tctgctcgcg gatggcgcgc agcgggaggt cgacgcccgc
      361 cgtcaggacg agggtctcga cgcgggagag cgcgtcgcgc ggcgagttcg cgtggatggt
      421 cgtgagcgag ccctcgtggc ccgtgttcat cgcctggagc atgtccaggg tctcggggcc
      481 gcggacctcg ccgacgatga tccggtcggg gcgcatgcgg agggcgttcc gcacgagctc
      541 gcggatccgc acctcgccct tgccctcgat gttcgccggg cgcgactcga gcgtgatcac
      601 gtgctcctgc tggagctgga gctcggccgc gtcctcgatc gtcacgatgc gctcgttccc
      661 gggcacgtag gccgagagcg cgttcagcgt ggtcgtctta ccggtgccgg taccgcccga
      721 gatgaggacg ttcagcttcc cgcgcacgca ggcggagagg aagtgggccg ccttcggcgt
      781 gagcgagccg aactcgatca ggtcgtccat cgtgaacggg tcgcgggaga acttccggat
      841 cgtgagcgtc ggcccgcgca gcgcgagcgg cgggatgatc gcgttcacgc ggctcccgtc
      901 cgggagacgc gcgtcgacca tcggcgacga ctcgtccacg cgccggccga cctgcgagac
      961 gatcttgtcg atgatccgga gcaggtgcgc gttgtcggcg aacgacgcgt tcgtccgctc
     1021 gagcttcccg ccccgctcga cgaagatctt gtcccagttg ttgaccatga cctcggtgat
     1081 cgagtcgtcg cggaggaacg gctcgagggg cccgtagccg aggatgtcgt ccgcgatctc
     1141 gcgcgtgatc cgccgccgct cctcgcgggt cagcggcgtc cggtcgagcg cgagctgctc
     1201 ggtgaccgtg cgcatgacct ggtcggagag gtcgcccgcg gcctccgtgg agaagagctg
     1261 cgggccgagc ttcgcgatgc acgcgtggtg gatgcgcgtc ttgagctccg cgtacgggtc
     1321 gagcgccgtc tcgggcgccg actcgcgcgg cgtcgcggcc gcgagcgcgg catggggctc
     1381 ggcgacggcg ccgttcgcgc cgccaccgtt cgccccggtg ccgttcgagc cgctcttgat
     1441 gcggtcgtgg aggcccatct acgcggcctt tcggaacagc cggcgcccgg tcttgccgtc
     1501 gcccttgtcg agcgggacga ccgacttcga gatctcgcgc acggccttcg agaagtccgc
     1561 gcgggagtcc gcgagcacgg ccgggttgcc gcggttcacg gcgatcggca cggcccggtc
     1621 ggacgggacg ctgaagcgca ccttgacctc gagcgcgctc tcgacctcgc cgggcttcat
     1681 gcccaccttc gagttggcgc ggttcaggac gaggctgatc cggtcggtcg ggaacgagag
     1741 cagctcgagc gtctggagcg cgagccggac gttcttgagc gtcggcacgt cgagcgagca
     1801 gatgagcagg agctcgtccg tccggtcgag cgtcgcgagc atcggcccgt ggaagaacgg
     1861 cgaggtgtcg acgacgatca cgtcgtacga ctcccgcgcg acctccagga gctgcgccag
     1921 cttcgcctcc gtcacgagct cggcgtcctc ggggcgcagc ggcgccggaa ggatgtcgag
     1981 cccggacgcg tgccgggtca cgtagcccgc gagcttctcg gtgtcgagct cgccgggggc
     2041 gacgaccagg tcgtagatcg tcttctcggg ctcgatcccc agcatgatcg cggcgtcgcc
     2101 gaactggagg tcgaggtcga ggaggagggc cttcttgccc tcgtccttcg ccaccgaggc
     2161 ggccgtgttc gaggcgagga cggtcttgcc cgtcccgccc ttcgggctga agacggtcac
     2221 gatgcgcccg cgccggccgc cgccgccctg gagccgccgg cccgagtggc cggccttgcg
     2281 gatggcgaag atgaggttct cggtcatctg cgggaggagc aggacgtcgg cgacgtccgc
     2341 ctcgagcgcc tcctcgagga ggttcgaggc ctcgcccgag gcgaggagga tcacgggcgc
     2401 ctgcgtgtgc tcgcggatcg ccgcgagctc gccctgcggg agcgtcgagc tccgcgtgcc
     2461 gtgcaggacg acgccgaggt ggccgccgcg gagggccgga gcggcctcgc cgagcgaggc
     2521 ggccgcgccg acgaactcca cctcgtggtg gcgctcgagg gcgtgccgga gctcctggag
     2581 cccctcgcag tcgccggtca tgaagatgcg cgtcttctcg cgcgtgccgg tggtctgggt
     2641 ctcgaggttc acgttactcg cctccttcca ggagcctcgc ggacatggcc tggaacctgg
     2701 cccgcccgat tccgtccagc aggagcgact cggccgactc ggcgctctcg gggctgtccg
     2761 ccgcgtccgt cgccgggcgc agctggagcg accaggtgcc gttctgcgtc agccagaaga
     2821 gcttctgcgc ctgcgcgtcg gtgagcgcga ggatcgtcga gtatccgccc gacgccgcct
     2881 gcgggttcgt gaccgactcc gtcgtgacgc cgccttcggg cgcctcgagg acgagcaggt
     2941 cgcgaagcac gacgcgcgag tagtggttcg tctcgctctc ggggacgttc cagctgccga
     3001 ccacgtcgac gcggtcgccg gcgacgagcg tccctgcgag gagctgctcg gcggtgccgg
     3061 ggacggagag cgcccgctcg ttgccctcga gctgcgcgcg gatgcccttc gcctcctcgg
     3121 tcgtgaaccg ccgggtggtc acctgctcgc cggcgtagat cgtcgcggag gcaacgcgcc
     3181 cgtcgagctg ctccggcgcg gagatcgcgc ccggcgtcac gttgcgcttg gcgacctcct
     3241 gctcggtgag gaacccctgc gcttcggcgc cggaggttcc cgccgggatg tcctgggagg
     3301 cgacgaacac cgtgacgttt gcctcggcgt ttcgcacctc cttcttgtaa ttcgtcacgt
     3361 agaagaccgt caacagcccc gcgacgagcg cgagcgcgat tgcgatcccg atgtttcgca
     3421 gcctgtacgt catgtgtgac ccgctttcct ttcctgtttc ctattcgacc agcgacacgg
     3481 tgaacgcccc gaacgacggt tggctcggag acgacgatgc aattccctcc cacatcacgc
     3541 gcacgaagcg gccgttcacg cgaccggtgc tcccaccggc gtggaagctc gtcgggacga
     3601 acccgaccca gccgatgatg tcgtagcgcg cgttcgaccc cgggcccgag atcgtccggt
     3661 agatcgggaa gaggacctcg gtattgaggc gcgagccgag cgcgctctgg aactggccgt
     3721 tgttgaagtt cgtcgacggg gcctgccagt agttgccgag gcccatctcg ccctcgaaac
     3781 ccgtctccat ccagtcggcg agcgtggacg cgccgacgtt cccgttcgga tcgttgcggt
     3841 tcaggttgat gagcccgaat gcgccggccg cgtcgccgcc gcccgggctg tgcaggttca
     3901 tgaggtcgat ctgcgtcggg ctcgtgcacg gcggcggcgt gcactggagc atcgggtgct
     3961 gccagttcac gacgatcggc gccacccagc gagccgccga gagcacgccg gtccgcgcct
     4021 tggcggtcgc gtgcacctcg accgagtcga tcccgaagag cttcgtgaag atccccggcg
     4081 cgtccttctc ggtctggacg acgatcgtgt cgttcgcgag gacgcccgtc tcgtaggtga
     4141 tgtcctcggc gccgaccccg ccgccgttct tgtccccgta ctcgatcgcg cgcgtggtcg
     4201 cgagcgccgt cgagtcgggg agctcctgcg cggccgcgag ggccgccgcg tcggtcgtcg
     4261 cctggagctt gcgatcctcg ttgaaccaga cgccgacgtc gagcacggcg cccgtcatcc
     4321 cgaggaggac ggtgaggaag agaacggtcg tcaccgtcgc ctggccggtc tgcctgcggg
     4381 cgcgcgtcat tcgatccgct cccgcgtcgt gctcgacacg ttgccggact tcacgacgaa
     4441 gccgagcagc gagatctcgt acgggtagga cgtcgtcacg atcacgtcgg cccctttctc
     4501 ccagcgcggc gacgccgtga ccgccacctg gaacttcgac tggtcgaggt tctgcgcgga
     4561 cgcccgtgca gcggtcgtgg ccgcgctcga ggggctgccg ccgccgtaca tcgcggtcgc
     4621 gcccttgcgg gcggcggcgc gcgcggcgtc cgtcaccgtc aggtagttgt tgaacgcgat
     4681 gccgcactga atgatcgcga agagcagcag cgcgatcacg ggcaggacga gagcgaactc
     4741 cgtcatcgtc tgcccgttct gtgcgcgaac gcgcgcggcg tgaattctgc ggaacatttg
     4801 cggcggaaaa tccttgtctg cggcggttga cttggagaag cgatgggcgg gctctccgcg
     4861 aaggagagcc cgcccggcca tgtgcctacc tctttgcggc ttagaggaag ccgacgaccc
     4921 ggttgatcgc gtcgctgatc gcacccgaga gggtgagaag cgcggcgacg acaccgatag
     4981 cgatgacggc caggaccacg ccgtactccg ccatggtctg accctcctca cccttccgaa
     5041 gcgtgttcag gtaggagaag aagctcgtca aagtgttcac ctcccttctt cgaactcgag
     5101 cggcccgaat cgagctcgtg agaccgatga aggtcgagcg gcgagcgcga gccgtggtgg
     5161 tgggggagag cccttcgttt cggcggatga catgcgtggt cacggtggaa gaaaacctcc
     5221 gtagatgcct ccggcgtgac cgctccgccg aggtggctca gatgcgagga ctatcgcccc
     5281 ggttccgccg gcctcacatc cctcgacgtg gtgattcgac taccggttcc tctattcgag
     5341 ggatgccacc gcgggcggcg ctttcgatag tggagccaag tccgtagcgg cgcggacggg
     5401 tcagatcgcg aaatcgcgcc gacctcgcgg actcggcgcc cgagtcagta ggagctcccc
     5461 caatgccaca accgagatcc tcttcgcgga cgccgctcgc gaggctcgtc ttcttcctcg
     5521 tcctgagctg ggcgctcgtc gccctcggga accggaaggg cgagatcgcc gagcacgccg
     5581 tgcccgagcg ggccgagccc gtcggaaaga ttcccgcgcg cgcgcgccgg cgctcgcgcc
     5641 gccagcggct cgccacgagc ctggccttca ccacgctctt cttcgcaggc gccgcgctct
     5701 ccgccggggc gggcgacatg ctcgtggagg cgatggacac gaccgggacc gacacgacgg
     5761 agacgaccga gaccaccccg gccgaggacg cagcggccga cggggagacg cccgccgacg
     5821 atgcgacggc cggcgagacg ccgaccgag
//
LOCUS       DASTFC010000214         6029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1569898,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000214 DASTFC010000000
VERSION     DASTFC010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569898"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFO88_06405"
     CDS             <1..1108
                     /locus_tag="VFO88_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9545191.1"
                     /translation="IITGLVLVVVSLLAAGCFHSDDENGNGNGEAAQTGTDGDTGAQE
                     GGGVFQQVQDRGNVVCGVNDAVPGFGTATEAGEYEGFDIDYCRAVAAAVLGDANMVEF
                     RTLTADQRFTALQSGEVDVLIRNTTYTASRDGAEGARFLHTTYYDGQGMMVTEESGFT
                     ELEDLAGSTVCVLSGTTTELNLETVFQARGIDYTPLTFEENEPLQQAFIQGRCDAWTS
                     DISQLAGVRSAWPDAEGGPDSLLIMDEIMSKEPLGPVVRDGDDDWADAVNWAVIVTIQ
                     AEELEITSENVQEMAGSDDPEIQRLLGQPVEGEPFDAGLGLDAEFALNVIEQVGNYGE
                     IFDRHLGPDSPLALDRGLNALWTDGGLQYAPPYR"
     gene            1165..2337
                     /locus_tag="VFO88_06410"
     CDS             1165..2337
                     /locus_tag="VFO88_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9545192.1"
                     /translation="MAAASPTAQVKPPFWRNVRVLRWAFQAVFLFLVIVFLLYLWGNL
                     TGNLERLGIRTDFRFLRQPAGFAIPDSDFRSSQSVWDAFLVGARNTAVVSILGIVFAT
                     ILGVLVGVARLSSNWLVRRAAALYVETLRNIPVLIIIIFWWAGILIRLPSLDENELFG
                     LVNLSNRGLVLPWLDRTGDLTLFLVVVLVTTVAAALVIRWRTRRFDATGQPHHRVLWG
                     LGVALAGVTLAYLVAGRPFTTSLPEVGSFGTTGGFRLSPEFGALLIALTLYTASHIAE
                     IVRGSILSVQQGQSEASNALGLSGFQRLRHVVLPQAFRVSVPPLANQYLNLTKNSSLG
                     IAIGYYELTRIARISISQAAPAPQATLVLMLFYLAFSLFIALVTNIVNRRLEIKGR"
     gene            2334..3605
                     /locus_tag="VFO88_06415"
     CDS             2334..3605
                     /locus_tag="VFO88_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET9545193.1"
                     /translation="MTATRPYVEEPLPPPEPEGPPILRIGPRRWLKENLFSTWFNTLL
                     TVVFAVLLSYAAYKLARFVLVDARWEVVQRNITNFMVFHFPRAELWRVWVALFVLAAA
                     AGLGVGAAGTRREAEVAEGRGGTPSTARGLVFRRVAPLVALVVVLLLAARSLTAAVLV
                     LGVAVTAIVFRLLGRRLPARAFRFVLLAVLAAIVAAFLVIIAFGGVPWDDWGGLLLTV
                     FLAVAGIVISFPLGVLLALGRRSKLPVIKLVCVVYIELIRGVPLITILFMAVFALGFF
                     LPAGTDPPSTVARALVGLILFTAAYVAEIVRGGLQSVPRGQIEAAQALGLSPSKTTRL
                     ILLPQALRAVIPALVGQFISLFKDTSLVFVVGLTELLAVAEIVTQQPDFRAQGLIVET
                     LLFVAFIYWAGSYWMSRESQRLEQRLGVGIR"
     gene            3602..4375
                     /locus_tag="VFO88_06420"
     CDS             3602..4375
                     /locus_tag="VFO88_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET9545194.1"
                     /translation="MTEVLTSAATVPVTGEPIIEVMDVSKWFDDFQALKDVDLTVGRQ
                     EVVVVCGPSGSGKSTLIRCINRLEKHDKGRIVVDGIELSDDIRNIGEIRREVGMVFQS
                     FNLFPHLTVLENVTLAPRQVRRKPKKEAQEKGMEVLTRVKIPEQAAKYPGQLSGGQQQ
                     RVAIARALAMEPKIMLFDEPTSALDPEMIKEVLDAMEELAESGMTMIVVTHEMGFARH
                     VADRMVFMADAEIVEAGTPEHFFENPREERTKQFLAQIL"
     gene            complement(4378..4971)
                     /locus_tag="VFO88_06425"
     CDS             complement(4378..4971)
                     /locus_tag="VFO88_06425"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HET9545195.1"
                     /translation="METVESYLAQPVEALLDGIASETPAPGGGSVSALVVAMAAALVA
                     MAARFSIGHWDEAEAAAARADELRRTTAPLAPGDSAAYEEVLTAMRLPTNLEPEVRNK
                     TIGNALARAAEIPLEIAGRAARVAELAAQVAEHGNPNLRGDAVAGALLAAGAARASAN
                     LVEINLGTTASDERVAIARARVAAAREAADRALALGP"
     gene            complement(5097..>6029)
                     /locus_tag="VFO88_06430"
     CDS             complement(5097..>6029)
                     /locus_tag="VFO88_06430"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET9545196.1"
                     /translation="GAMTVLLKDAIKPNLVQTLEGQPAFLHCGPFANIAHGNNSLVAT
                     RVGLKLGEFCVTESGFGSDMGMEKFFDIVCRVGELRPDAVVLVATARALKHHGDDPDG
                     GVEAIERGAANMVRHLGIVKEFGLNAVVAVNRFPGDTEEEIEAVRRLAVEGGAMAAEV
                     NEAFERGGEGATALAEAVVAAAEAPSEFRPLYELDETIEAKIEAICTRVYGADGVVFL
                     PAAQQKIEQFVANGFDKLPICMAKTHLSLSHDPTLVNAPSGFTVTVRDIRQYTGAGWL
                     VPLLGDMQTMPGYGVSPAAFDVDIDETGKTVGLF"
BASE COUNT          817 a         2146 c         2099 g          967 t
ORIGIN      
        1 gatcatcacg ggcctggtgc tcgtggtcgt gtcgctgctc gcggccgggt gcttccattc
       61 ggacgacgag aacggcaacg gaaacgggga ggccgcgcag acaggcacgg acggggacac
      121 cggcgcgcag gagggcggcg gtgtcttcca gcaggtgcag gaccgcggca acgtggtctg
      181 cggcgtgaac gacgcggtgc cgggcttcgg gaccgcgacg gaggccgggg agtacgaggg
      241 cttcgacatc gactactgcc gcgcggtcgc ggcggccgtg ctcggcgatg cgaacatggt
      301 cgagttcaga acgctcaccg cggaccagcg cttcaccgct ctccagtcgg gcgaggtgga
      361 cgttctcatc cgcaacacga cctacacggc cagccgtgac ggcgccgagg gcgcgcgctt
      421 cctccacacg acgtactacg acggccaggg gatgatggtc acggaggaga gcgggttcac
      481 cgagctcgag gacctcgcgg gctcgacggt ctgcgtgctc tcgggcacga ccaccgagct
      541 caacctcgag accgtcttcc aggcgcgcgg gatcgactac acgccgctca cgttcgaaga
      601 gaacgagccg ctgcagcagg cgttcatcca gggacgctgc gacgcgtgga cctcggacat
      661 ctcccagctc gccggcgtgc gttcggcgtg gccggacgcc gagggcgggc ccgacagcct
      721 cctcatcatg gacgagatca tgtcgaagga gccgctcggg ccggtcgtcc gcgacggcga
      781 cgacgactgg gccgacgcgg tcaactgggc cgtgatcgtg acgatccagg cggaggagct
      841 cgagatcacg tcggagaacg tccaggagat ggcgggctcg gacgacccgg agatccagcg
      901 gctgctcggc cagcccgtcg agggcgagcc gttcgacgcg ggcctcggcc tcgacgccga
      961 gttcgcgctg aacgtgatcg agcaggtcgg gaactacggc gagatcttcg accggcacct
     1021 cgggccggac tcgccgctcg cactcgatcg cgggctcaac gcgctctgga cggacggcgg
     1081 cctccagtac gcgcctccat accgctgacc gattagggtg gggcggcggc gtcggccgcc
     1141 gccccaccaa cgccttcgac gaccgtggct gcagcctctc cgaccgcgca ggtgaagccg
     1201 ccgttctggc ggaacgtccg cgtgctgcgc tgggcgttcc aggccgtctt cctcttcctc
     1261 gtcatcgtgt tcctcctgta cctctggggg aacctgacgg gcaacctcga gcgcctgggg
     1321 atccggacgg acttccgctt cctccggcag cccgccgggt tcgccatccc cgactcggac
     1381 ttccgctcgt cgcagagcgt ctgggacgcg ttcctcgtgg gcgcgcggaa cacggccgtc
     1441 gtctcgatcc tcgggatcgt cttcgcgacg atcctgggcg tcctcgtcgg cgtcgcacgg
     1501 ctctcctcga actggctcgt gcggcgcgct gcggcgctct acgtggagac gctccggaac
     1561 atccccgtcc tcatcatcat catcttctgg tgggccggga tcctcatccg gctgccgtcg
     1621 ctcgacgaga acgagctgtt cggcctcgtc aacctgtcca accggggcct cgtcctgccc
     1681 tggctcgacc ggaccggcga cctgaccctg ttcctcgtcg tcgtgctcgt cacgacggtc
     1741 gcggcggcgc tcgtcatccg ctggcggacc cgccgcttcg acgcgacggg ccagccgcac
     1801 caccgggtgc tctgggggct cggggtcgcc ctcgccggcg tcacgctcgc gtacctcgtc
     1861 gccggccgcc cgttcacgac ctcgctgccc gaggtcgggt cgttcggcac gacgggcggc
     1921 ttccggctca gcccggagtt cggcgcgctc ctgatcgcgc tcaccctcta caccgcgagc
     1981 cacatcgcgg agatcgtccg cgggagcatc ctctcggtgc agcagggcca atccgaggcc
     2041 tcgaacgcgc tcgggctcag cggcttccag cgcctgcgtc acgtcgttct gccgcaggcg
     2101 ttccgcgtgt ccgtgccgcc gctcgcgaac cagtacctca acctgacgaa gaactcctcc
     2161 ctcggcatcg cgatcggcta ctacgagctg acgcggatcg cgcggatctc gatcagccag
     2221 gccgcgcccg cgccgcaggc gaccctcgtc ctcatgctct tctacctcgc gttctcgctc
     2281 ttcatcgcgc tcgtcacgaa catcgtcaac cgacgcctgg agatcaaggg ccgatgacgg
     2341 cgacgcgccc gtacgtcgaa gagccgctgc cgccccccga gccggagggc ccgccgatcc
     2401 ttcgcatcgg cccgcgccgc tggctgaagg agaacctctt cagcacgtgg ttcaacacgc
     2461 tgctcaccgt cgtgttcgcc gtcctgctct cctacgcggc gtacaagctg gcgcgcttcg
     2521 ttctcgtgga cgcgcggtgg gaggtcgtcc agcgcaatat cacgaacttc atggtcttcc
     2581 acttcccgcg cgcggagctc tggcgcgtat gggtcgccct cttcgtgctc gccgccgcgg
     2641 ccgggctcgg ggtcggggcg gcggggacac gccgcgaggc ggaagtggcg gagggccggg
     2701 gcggaacgcc ctcgacggcc cgcggcctcg tcttccgccg cgtcgcgccg ctcgtcgcgc
     2761 tcgtcgtcgt gctcctgctc gcggcacggt cgctgaccgc ggcggtgctc gtgctcggcg
     2821 ttgcggtaac cgcgatcgtc ttccggctcc tcggccggcg cctgccggcc cgcgccttcc
     2881 gcttcgtcct gctcgcggtg ctcgccgcga tcgtcgcggc cttcctcgtc atcatcgcgt
     2941 tcggcggcgt cccctgggac gactggggcg gtctcctcct gaccgtcttc ctcgccgtcg
     3001 cggggatcgt catctcgttc ccgctcggcg tgctgcttgc gctcggccga cgctcgaagc
     3061 tcccggtaat caagctcgtc tgcgtcgtct acatcgagct gatccgtggc gtccccctga
     3121 tcacgatcct tttcatggcg gtgttcgcgc tcgggttctt cctcccggcc ggcacggatc
     3181 cgccgagcac ggtcgcgcgg gcgctcgtcg gcctcatcct gttcaccgcg gcctacgtgg
     3241 cggagatcgt gcgcggcggc ctgcagtccg tgccgcgtgg gcagatcgag gccgcgcagg
     3301 cgctcggcct ctcgccgtcg aagaccacgc ggctcatcct cctgccgcag gcgctgcgag
     3361 cggtcatccc ggcgctcgtc gggcagttca tcagcctctt caaggacacg tcgctcgtgt
     3421 tcgtcgtcgg gctgacggag ttgctcgcgg tggcggagat cgtcacgcag cagccggact
     3481 tccgcgccca gggcctcatc gtggagacgc tgctgttcgt cgcgttcatc tactgggctg
     3541 gttcatactg gatgtccagg gagagccagc gactcgagca acggctgggg gtgggaatca
     3601 ggtgaccgag gttctgacat cggcggcgac ggtccccgtg acgggcgagc cgatcatcga
     3661 ggtcatggac gtctcgaagt ggttcgacga cttccaggcg ctcaaggacg tcgatctcac
     3721 cgtgggccgg caggaggtcg tggtcgtctg cgggccgtcc ggctcgggca agtcgacgct
     3781 catccgctgc atcaaccggc tcgagaagca cgacaaggga cggatcgtcg tggacgggat
     3841 cgagctctcg gacgacatcc ggaacatcgg agagatccgg cgcgaggtcg ggatggtctt
     3901 ccagtccttc aatctcttcc cgcacctgac cgtgctcgag aacgtcaccc tcgctccgcg
     3961 ccaggtgcgc cgcaagccga agaaggaggc gcaggagaag gggatggaag tcctcacgcg
     4021 cgtgaagatc ccggagcagg cggcgaagta cccgggccag ctctccgggg ggcagcagca
     4081 gcgcgtggcg atcgcgcggg cgctcgcgat ggagcccaag atcatgctct tcgacgagcc
     4141 gacctccgcg ctcgacccgg agatgatcaa ggaggtgctc gacgcgatgg aggagctcgc
     4201 cgagtcgggg atgacgatga tcgtcgtcac gcacgagatg ggcttcgcgc gccacgtcgc
     4261 cgacaggatg gtcttcatgg cggacgccga gatcgtggag gcggggacgc cggagcactt
     4321 cttcgagaac ccgcgggagg aacggacgaa gcagttcctc gcgcagattc tgtagggcta
     4381 cgggccgagg gcgagggcgc ggtcggcggc ctcgcgggcg gccgcgacgc gcgcgcgggc
     4441 gatcgcgaca cgctcgtcgg aggcggtcgt cccgaggttg atctcgacga ggttcgcgct
     4501 cgcgcgcgcg gcgccggcgg cgaggagcgc acccgcgacg gcgtcgccgc ggaggttcgg
     4561 gttgccgtgc tcggcgacct gcgcggcgag ctcggcgacg cgcgcggccc ggccggcgat
     4621 ctcgagcggg atctccgcgg cgcgggcgag cgcgttgccg atcgtcttgt tgcggacctc
     4681 gggctcgagg ttcgtcggaa gccgcatcgc ggtcaggacc tcctcgtacg ccgcgctgtc
     4741 gcccggcgcg agcggggcgg tcgtgcgccg gagctcgtcg gcgcgcgcgg cggccgcctc
     4801 ggcctcgtcc cagtggccga tcgagaaccg cgcggccatc gccacgagcg ccgccgccat
     4861 ggcgaccacg agggcggaca cggagccccc accgggcgcg ggcgtctcgg acgcgatgcc
     4921 gtcgagcagc gcctcgacgg gctgcgcgag gtagctctcg acggtctcca tcggcggatt
     4981 ctgacgcgtc cccgtgcgcg atgccacttg tctcggcaag caacgtgtcc gcgcgggcca
     5041 cgtccggtgc caggcacggg gcgtgcccgg cgtggccgcg gccgaatcgc cgacggctag
     5101 aagagcccga ccgtcttgcc ggtctcgtcg atgtcgacgt cgaacgcggc gggcgagacg
     5161 ccgtagccgg gcatcgtctg catgtcgccg agaagcggga cgagccaccc ggcacccgtg
     5221 tactggcgga tgtcgcggac ggtcacggtg aagccgctcg gcgcgttcac gagcgtcggg
     5281 tcgtgcgaga gcgaaaggtg cgtcttcgcc atgcagatcg gcagcttgtc gaagccgttc
     5341 gccacgaact gctcgatctt ctgctgcgcc gccgggagga agacgacgcc gtcggcgccg
     5401 tacacccgcg tgcagatcgc ctcgatcttc gcctcgatcg tctcgtcgag ctcgtagagc
     5461 ggccggaact cggacggcgc ctcggccgcg gcgacgaccg cctcggcgag cgccgtcgcg
     5521 ccctcgccgc cgcgctcgaa cgcctcgttc acctcggccg ccatcgcgcc gccctcgacc
     5581 gcgagccggc gcaccgcctc gatctcctcc tccgtgtcgc cggggaaccg gttcacggcc
     5641 acgaccgcgt tcaggccgaa ctccttcacg atcccgaggt ggcgcaccat gttggccgcg
     5701 ccgcgctcga tcgcctcgac gccgccgtcc gggtcgtcgc cgtggtgctt cagcgcgcgc
     5761 gccgtggcca cgagcacgac cgcgtccggc cggagctccc cgacccggca cacgatgtcg
     5821 aagaacttct ccatccccat gtcggagccg aaccccgact ccgtcacgca gaactcgccg
     5881 agcttgagcc cgacgcgcgt cgcgacgagc gagttgttcc cgtgcgcgat gttcgcgaag
     5941 gggccgcagt ggaggaaggc cggctggccc tcgagcgtct ggacgaggtt cggcttgatc
     6001 gcgtccttca ggagcacggt catcgcgcc
//
LOCUS       DASTFC010000215         5265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1191122,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000215 DASTFC010000000
VERSION     DASTFC010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191122"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFO88_06435"
     CDS             <1..195
                     /locus_tag="VFO88_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545197.1"
                     /translation="PEIELAHADAQARGIATGDTVTVGLNGSGRPLRASVSRKLRPGV
                     ARIAAEHARGLGSHVEVTKA"
     gene            192..1199
                     /gene="nuoH"
                     /locus_tag="VFO88_06440"
     CDS             192..1199
                     /gene="nuoH"
                     /locus_tag="VFO88_06440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET9545198.1"
                     /translation="MTPALAQALTEPWWISVIKAFVIANLVLGTFAYLTVVERKLLGR
                     MHLRYGPNRVGPFGLLQPIADLMKLLRKASFFPKGAVTILYILAPILAAFTAISAFAV
                     IPFGPGWEIGGYYVAGQIVDVSIALLLILALSAVGIYAFVVGGWASDSKYSLLGSMRT
                     CAQLVSYEVSFGLAVIGVVIMAGSLRLTEIVAAQQEQIWFIVPQLVGFLCFFVAGVAE
                     TNRAPFDLPEAEQELVAGYHTEYGGIRWGMFQMSEYINMLTFSALGVTLFFGGWSGPI
                     LPAPIWFLLKLGALIFVFMWLRATLPRLRYDQLMKFGWKVLLPVATLNAIATAVLVTI
                     F"
     gene            1210..1707
                     /gene="nuoI"
                     /locus_tag="VFO88_06445"
     CDS             1210..1707
                     /gene="nuoI"
                     /locus_tag="VFO88_06445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET9545199.1"
                     /translation="MSPQPLPSLKGFAVTLRQIFRKPITQEYPEYKRPVYPRFRGRHR
                     LNLHDNGLEKCVGCSLCAAACPADCIRVVPAENTPENRVSPGERYARIYEINMSRCIF
                     CGYCELACPFDAITLGNEYELSEYHRDDQIYTKEMLLAPPIKQVPVADRERYDTPEPV
                     WKTES"
     gene            1712..2275
                     /locus_tag="VFO88_06450"
     CDS             1712..2275
                     /locus_tag="VFO88_06450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9545200.1"
                     /translation="MDAGEILVWIVWIGAATALFASGLAVVLFQNPFYSALSLIVNLA
                     SLAVLFLLLSAEFVAAAQVLVYAGAVVVMFLFVTAYLGGRADAPWAGGPTLQSFFAVL
                     AAGAIAVEVVVVIALTAGDRLTDPAEIADAFGSPAEIGRLFISDHLLAFEATSIVLLV
                     AAVGGVILGTTAPRSVRAEGRAEVPEP"
     gene            2272..2580
                     /gene="nuoK"
                     /locus_tag="VFO88_06455"
     CDS             2272..2580
                     /gene="nuoK"
                     /locus_tag="VFO88_06455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9545201.1"
                     /translation="MMGPGIASYLVVSVVLFSIGAFGVLIRRSPLIVLLALEIMLNAG
                     NLALLAFARHLGDGDGHVFALAVMVVAASEAVIGLGLIVAMSRLKVELDVDKLTTLRG
                     "
     gene            2584..4488
                     /gene="nuoL"
                     /locus_tag="VFO88_06460"
     CDS             2584..4488
                     /gene="nuoL"
                     /locus_tag="VFO88_06460"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9545202.1"
                     /translation="MDVAAWIALFSPAVGVAAITLLGHRITGAQAGILASGSVLVSFA
                     ASVVAFVVLAGRPEEERTELSTLWTWLSTGDLEVGLEVLVDPLSVFMMLVVSGVGFLI
                     LTYATGYMRGDAEERRYHAYKALFVFSMLLLVQGGNLLLLLAGWGMVGLSSYLLINFW
                     HERPTAVAAGKKAFVMNAFGDATFVLALFVIVAQTGVLGFAPVFERAPEALGESTTLS
                     VLVALGLLGGAVAKSAQIPLHTWLPDAMEGPTPVSALIHAATMVTAGVYLIARTAPLF
                     ELAPAIHDLAAILGGVTIVFAGLIALVQTDIKRVIAYSTMSQIGYMFVAAGLGAYGAG
                     MFHLMTHAFFKALLFLAAGVVIHALAGEQDIRRMGGLGRELPWTTRFFVIGALALAAI
                     PPFAGFFSKDAILASAANAGALGWMLWTLGAIGAFLTALYTFRLVFVVFGPPITDFAR
                     DHLHRERFEGPLAMMWPVAILAVLSLVGGFLQVPGLWHLVDDWLHPVAESIEEAHGWV
                     AWFSALAALGLALGGIALAWLLWGKRSDVPERVRAGAPWLAAAPERKFWFDEAYDGVF
                     AWPVRMVASGLGRFVERPVFLEPLGDVGTGMRGLSGRLGAVQTGGVRTYVLMLALGLA
                     VLTVVFLAVS"
     gene            4652..>5265
                     /locus_tag="VFO88_06465"
     CDS             4652..>5265
                     /locus_tag="VFO88_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9545203.1"
                     /translation="MLSTALVFLPIAGALLTWLIPWASVRAAGGFALLVALAQLGLWT
                     VALGRMDFGSGALQEATETPWFEDLGVAYKVGLYDFSLWLVGLTALVTVAAIAYGTWA
                     GRERPRAYFGSLLFLAGATTGVFVAQDLLLFYVFFEAMLIPLYVLIGVWGGPRRGAAT
                     LKFIVYTMAGSLLMLVSVIALGLGNGTFDLTELSASTNHWIFLG"
BASE COUNT          605 a         1919 c         1795 g          946 t
ORIGIN      
        1 cccgagatcg agctcgcgca cgcggacgcg caggcgcgcg ggatcgcgac cggcgacacg
       61 gtgaccgtcg ggctgaacgg ctccggccgg ccgctccgcg cgtccgtcag ccgcaagctt
      121 cggcccggcg tcgcgcgcat cgccgccgag cacgcgcgcg gtctcggctc gcacgtcgag
      181 gtgacgaagg cgtgacgccg gcgctcgccc aggcgctcac ggagccctgg tggatcagcg
      241 tcatcaaggc gttcgtgatc gcgaacctcg tcctcggcac gttcgcctac ctgacggtcg
      301 tcgagcggaa gctcctcggg cgcatgcatc tgcgctacgg gccgaaccgc gtcgggccgt
      361 tcggccttct ccagccgatc gccgacctca tgaagctgct gcggaaggcg agcttcttcc
      421 cgaagggcgc ggtcacgatc ctctacatcc tcgccccgat cctcgctgca ttcacggcga
      481 tcagcgcgtt cgccgtcatc cccttcgggc ccggctggga gatcggcggc tactacgtcg
      541 cggggcagat cgtggacgtc tcgatcgcgc tcctcctcat cctcgcgctg agcgccgtcg
      601 ggatctacgc gttcgtcgtc ggcggctggg cgagcgactc gaagtactcg ctcctcggct
      661 ccatgcggac ctgcgcgcag ctcgtctcgt acgaggtgtc gttcgggctc gccgtgatcg
      721 gcgtggtcat catggccggc tcgctgaggc tcacggagat cgtcgccgcc cagcaggagc
      781 agatctggtt catcgtcccg cagctcgtcg gcttcctctg cttcttcgtc gccggcgtcg
      841 cggagacgaa ccgcgctccg ttcgacctcc ccgaggcgga gcaggagctc gtcgcgggct
      901 accacaccga gtacggcggg atccgctggg ggatgttcca gatgtccgag tacatcaaca
      961 tgctcacgtt ctcggcgctc ggggtcacgc tcttcttcgg cggctggagc gggccgatcc
     1021 tccccgcgcc gatctggttc ctcctgaagc tcggggcgct catcttcgtc ttcatgtggc
     1081 tgcgcgccac gctgccgcgc ctgcgctacg accagctcat gaagttcggc tggaaggtgc
     1141 tccttccggt cgccaccctc aacgcgatcg ccaccgccgt tctggtcacg atcttctagc
     1201 ctgccgccga tgtcgcctca gccgcttccg tcgctcaagg gattcgccgt cacgctgcgg
     1261 cagatcttcc gcaagccgat cacgcaggag taccccgagt acaagcgccc ggtctacccg
     1321 cgcttccgcg ggcggcaccg gctgaacctg cacgacaacg ggctcgagaa gtgcgtcggc
     1381 tgctcgctct gtgccgccgc ttgcccggcg gactgcatcc gggtcgtccc ggccgagaac
     1441 acgcccgaga accgcgtctc gccgggcgag cgctacgccc gcatctacga gatcaacatg
     1501 agccgctgca tcttctgcgg ctactgcgag ctcgcctgcc cgttcgacgc gatcacgctc
     1561 ggcaacgagt acgagctctc cgagtaccac cgcgacgacc agatctacac gaaggagatg
     1621 ctcctcgcgc cgccgatcaa gcaggtcccg gtcgccgacc gcgagcgcta cgacaccccc
     1681 gagccggtct ggaagacgga gtcctgagcc agtggacgcc ggggagatcc tcgtctggat
     1741 cgtgtggatc ggcgcggcga cggcgctctt cgcgtcgggg ctcgcggtcg tcctcttcca
     1801 gaacccgttc tactcggcgc tctcgctcat cgtgaacctc gcgtcgctcg cggtgctctt
     1861 cctgctcctc tcggccgagt tcgtcgcggc agcgcaggtg ctcgtctacg cgggggcggt
     1921 cgtcgtcatg ttcctcttcg tgaccgccta cctcggcggc cgcgcggatg cgccctgggc
     1981 gggcgggccg acgctccaga gcttcttcgc cgtcctcgcc gcgggcgcca tcgccgtcga
     2041 ggtcgtggtc gtgatcgcgc tcaccgccgg cgaccggctg accgacccgg cggagatcgc
     2101 cgacgcgttc ggctcgccgg ccgagatcgg gcggctcttc atctcggacc acctgctcgc
     2161 gttcgaggcg acctcgatcg tgctgctcgt cgcggcggtc ggcggcgtca tcctcgggac
     2221 gacggcgccg aggagcgtgc gcgccgaggg ccgcgcggag gtgccggagc catgatgggc
     2281 ccggggatcg cctcgtacct ggtcgtctcc gtcgtcctgt tctcgatcgg ggcgttcggc
     2341 gtgctcatcc gccgcagccc gctgatcgtg cttctcgcgc tcgagatcat gctgaacgcg
     2401 gggaacctcg ccctgctcgc gttcgcgcgg catctcggcg acggcgacgg gcacgtgttc
     2461 gcgctcgccg tgatggtcgt cgccgcgtcg gaggctgtca tcgggctcgg cctgatcgtc
     2521 gccatgtcgc gcctcaaggt cgagctcgac gtcgacaagc tgaccacgct gcgcggctga
     2581 cggatggacg tcgccgcctg gatcgcgctc ttctcgccgg ccgtcggcgt cgcggcgatc
     2641 accctgctcg ggcaccggat cacgggcgcc caggccggga tcctcgcgtc gggctcggtg
     2701 ctcgtctcgt tcgcggcgag cgtcgtcgcg ttcgtcgtgc tcgccggtcg ccccgaggag
     2761 gagcggaccg agctctcgac gctctggacg tggctctcga ccggcgacct cgaggtcggg
     2821 ctcgaagtcc tcgtcgaccc gctgagcgtc ttcatgatgc tcgtcgtctc cggggtcggg
     2881 ttcctcatcc tgacctacgc gaccggctac atgcgcggcg acgccgagga gcggcgctac
     2941 cacgcgtaca aggcgctctt cgtcttctcg atgctcctcc tcgtccaggg cggcaacctg
     3001 ctcctgctcc tcgcgggctg ggggatggtc gggctctcgt cgtacctcct gatcaacttc
     3061 tggcacgagc ggccgaccgc cgtcgcggcg ggcaagaagg cgttcgtcat gaacgcgttc
     3121 ggcgacgcga cgttcgtgct cgcgctcttc gtcatcgtgg cgcagaccgg cgtgctcggg
     3181 ttcgcgccgg tcttcgagcg cgccccggag gcgctcggcg agtcgaccac gctctccgtg
     3241 ctcgtggcgc tcgggctgct cggcggcgcc gtcgcgaagt cggcgcagat cccgctccac
     3301 acgtggctcc cggacgcgat ggagggcccg acgccggtct ccgccctcat ccacgcggcc
     3361 acgatggtga cggccggcgt gtacctgatc gcgcgcacgg cgccgctctt cgagctcgcg
     3421 ccggcgatcc acgacctcgc ggcgatcctc ggcggggtca cgatcgtctt cgcgggcctg
     3481 atcgcgctcg tccagacgga catcaagcgc gtgatcgcgt actcgaccat gagccagatc
     3541 ggctacatgt tcgtcgccgc ggggctcggc gcctacgggg ccgggatgtt ccacctgatg
     3601 acccacgcct tcttcaaggc gctcctcttc ctggcggccg gcgtcgtcat ccacgcgctc
     3661 gcgggggagc aggacatccg gcgcatgggc gggctcgggc gcgagctgcc gtggacgacg
     3721 cggttcttcg tgatcggcgc gctcgcgctc gcggcgatcc cgccgttcgc cgggttcttc
     3781 tcgaaggacg cgatcctcgc gagcgcggcg aacgccgggg cgctcggctg gatgctctgg
     3841 acgctcggcg cgatcggcgc gttcctgacc gcgctctaca cgttccggct cgtgttcgtc
     3901 gtcttcggcc cgccgatcac cgacttcgcg cgcgaccatc tccaccggga gcgcttcgag
     3961 gggccgctcg cgatgatgtg gccggtcgcg atcctcgcgg tgctctcgct cgtcggcggc
     4021 ttcctccagg tgcctgggct ctggcacctc gtcgacgact ggctccaccc ggtggccgag
     4081 tcgatcgagg aggcgcacgg ctgggtcgcc tggttctcgg cgctcgccgc gctcggcctg
     4141 gcgctcggcg ggatcgcgct cgcgtggctt ctctggggga agcgaagcga cgtgccggag
     4201 cgtgtgcgcg cgggcgcgcc gtggctcgcc gccgcgccgg agcggaagtt ctggttcgac
     4261 gaggcctacg acggcgtctt cgcgtggccc gtgcggatgg tcgcgagcgg cctcggtcgc
     4321 ttcgtcgagc ggccggtctt cctcgagccg ctcggcgacg tcggcaccgg gatgcgcggc
     4381 ctctcgggcc ggctcggcgc cgtccagacc ggcggcgtgc ggacgtacgt cctcatgctc
     4441 gcgctcgggc tcgccgtcct caccgtcgtc ttcctggcgg tgtcctagtg cccctcccgg
     4501 cagcactcct ccgcgtctgg cccgcgatca cgcgacgcga gaccccaccc tcgtccttgc
     4561 caaggcagcc agccttggct gcgaccgagt gcggcgtctc gcttggtgct cgcgacccag
     4621 ccgctagacg gcgccaccgg aaggggcact gatgctgagc accgcgctcg tcttcctccc
     4681 gatcgccggc gcgctcctca catggctcat cccgtgggcg agcgtgcggg cggccggcgg
     4741 gttcgcgctc ctcgtcgcgc tcgcgcagct cggcctgtgg acggtcgcgc tcggacggat
     4801 ggacttcggc tcgggcgccc tccaggaggc gaccgagacg ccgtggttcg aggatctcgg
     4861 cgtcgcgtac aaggtcgggc tctacgactt ctcgctctgg ctcgtcggcc tgaccgcgct
     4921 cgtcacggtc gcggcgatcg cgtacgggac ctgggccggg cgggagcggc cgcgcgcgta
     4981 cttcggctcg ctcctcttcc tcgccggcgc gaccaccggg gtcttcgtgg cgcaggacct
     5041 cctgctgttc tacgtcttct tcgaggcgat gctgatcccg ctctacgtcc tgatcggggt
     5101 ctggggcggg ccgcggcggg gcgccgcgac gctcaagttc atcgtctaca cgatggcggg
     5161 ctcgctgctc atgctcgtct ccgtcatcgc gctcgggctc gggaacggga cgttcgacct
     5221 gaccgagctg tcggcgagca cgaaccactg gatcttcctc gggtt
//
LOCUS       DASTFC010000216         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1290099,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000216 DASTFC010000000
VERSION     DASTFC010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290099"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VFO88_06470"
     CDS             <1..1572
                     /locus_tag="VFO88_06470"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9545204.1"
                     /translation="FGSIDATLLLATTVLILVLLLLIYRSPAVALVPLVVVALAYVVA
                     AGIVYALVSRAGLDVNGQATGILVILMFGAGTDYCLLIVSRFREELARVEDKHAAIEA
                     ALTHTSPAILSSGGTTSLALLTLLVADLGSIRSAGPVLALGIAVTMLAGLTLLPALLA
                     ALGRRSFWPAIPRVGAEERRRPGIWRRIGDVVARRPGVTLTVTVAALALASLGNLVSL
                     PGLSLASGFRATPESVEGFRALEEALPAGAAATTDVLVESDPARVDEASGAVAAALAG
                     VPGVEAVRPGERSEDGRIARLSVTLASDPYGDEAVDAILQLREAARDAAGEGATALVG
                     GATAEEADGRAAASRDASLVVPLTLLVVLLILVALLRALVAPLYLVASVVLSFAATLG
                     LTYALLEFVFDAPGTDASLPIFVFVFVVALGVDYNIFLVARIREETRRHGPREGVLRG
                     LERTGGVITSAGLILAGTFAVLMTLPLEQLFQLGFAVAIGILVDTFVVRTLVVPAIAL
                     LLGRWSWWPGRLGRA"
     gene            1600..>2018
                     /locus_tag="VFO88_06475"
     CDS             1600..>2018
                     /locus_tag="VFO88_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9545205.1"
                     /translation="MAVATDREYGLFIDGELVEPASGELRDLAEPATGDPLGRAAMAG
                     PDDVDRAVAAARAALSGPWGKTGPTERSRLLHALADAVVANRKELAELETRNVGKAIA
                     SVKAEVAVAEQNFRFFASVLGSLAGRSHPVGGSLLYYS"
BASE COUNT          196 a          793 c          717 g          312 t
ORIGIN      
        1 ttcggctcga tcgacgcgac gctcctcctt gcgacgacgg tcctcatcct cgtcctgctc
       61 ctgctcatct accgctcgcc ggcggtcgcg ctcgtcccgc tcgtggtcgt cgcgctcgcg
      121 tacgtcgtcg ccgcgggaat cgtctacgcg ctcgtctccc gggcggggct cgacgtgaac
      181 ggccaggcga ccgggatcct cgtgatcctc atgttcggcg ccggcaccga ctactgcctt
      241 ctgatcgtct cacgcttccg cgaggagctc gcccgtgtcg aggacaagca cgcggcgatc
      301 gaggccgcgc tcacgcacac gtcgcccgcg atcctctcga gcggcgggac gacgtcgctc
      361 gcgctcctca cgctcctcgt cgccgacctc ggctcgatcc gctcggccgg gccggtgctc
      421 gcgctcggca tcgcggtcac gatgctcgcc gggctcacgc tcctccccgc gctcctcgcg
      481 gcgctcggcc gccgctcgtt ctggcccgcg atcccgcgcg tcggcgcgga ggagcggcgg
      541 cgccccggga tctggcggcg gatcggcgac gtcgtggccc gccggccggg agtcacgctc
      601 accgtcacgg tcgccgcgct cgcgctcgcc tccctcggga acctcgtgtc gctgcccggg
      661 ctctcgctcg cgtcgggctt ccgcgcgacg ccggagtcgg tcgagggctt tcgggcgctc
      721 gaggaggcgc ttccggccgg cgcggcggcg acgaccgacg tgctcgtcga gtccgacccc
      781 gcccgcgtgg acgaggcgag cggcgccgtc gccgccgcgc tcgccggcgt gccgggcgtc
      841 gaggccgtcc ggcccgggga gcgaagcgag gacggccgca tcgcccgcct ctcggtcacg
      901 ctcgcgagcg acccgtacgg cgacgaggcg gtggacgcga tcctccagtt gcgcgaggcc
      961 gcgcgcgacg ccgccggcga gggcgcgacg gcgctcgtcg ggggcgcgac cgcggaggag
     1021 gcggacggcc gcgcggccgc gtcgcgcgac gcgtcactcg tcgtcccgct gacgctcctc
     1081 gtcgtgttgc tgatcctcgt cgcgctgctc cgcgcgctcg tggccccgct ctacctcgtg
     1141 gcgagcgtcg tcctctcgtt cgcggcgacg ctcgggctca cgtatgcgct gctcgagttc
     1201 gtcttcgacg cgcccggcac ggacgcgagc ctgccgatct tcgtcttcgt cttcgtcgtc
     1261 gcgctcggcg tggactacaa catcttcctc gtcgcgcgga tccgcgagga gacgcggcgg
     1321 cacgggccgc gtgagggcgt cctgcgcggg ctcgagcgga ccggcggcgt gatcacgagc
     1381 gcagggctca tcctcgccgg cacgttcgcc gtgctcatga cgctgccgct cgagcagctc
     1441 ttccagctcg gcttcgcggt cgcgatcggg atcctcgtgg acacgttcgt cgtccggacg
     1501 ctcgtcgtcc cggcgatcgc gctcctcctc ggccgctggt cgtggtggcc gggccggctc
     1561 ggccgcgcct gaccggcgcg ctccggttat cgtcccggca tggccgtcgc caccgaccgc
     1621 gagtacgggc tcttcatcga cggggagctc gtcgagcccg cctccggaga gctccgcgac
     1681 ctcgcggagc cggcgaccgg cgacccgctc gggcgcgcgg cgatggccgg gccggacgac
     1741 gtcgaccgcg ccgtcgcggc cgcacgcgcc gccctctccg gcccgtgggg caagacgggg
     1801 ccgaccgagc gctcgcggct cctccacgcg ctcgccgacg cggtcgtcgc gaaccgcaag
     1861 gagctcgccg agctcgagac gcgcaacgtc ggcaaggcga tcgcgagcgt caaggccgag
     1921 gtcgccgtgg ccgagcagaa cttccgcttc ttcgcgtcgg tgctcggctc gctcgccgga
     1981 cgctcgcacc cggtcggcgg ctcgctcctc tactactc
//
LOCUS       DASTFC010000217         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_713523,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000217 DASTFC010000000
VERSION     DASTFC010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713523"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFO88_06480"
     CDS             <1..708
                     /locus_tag="VFO88_06480"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HET9545206.1"
                     /translation="GREAGRFYSLRGGRLEEITDLSTETPGRHDQGGWSQARFQRHID
                     ELAAGHLRAVAEEIDRRVRRAGGGIDVVVVAPEESRAELAGYLTQDAEHALAGWALAE
                     AHAGPTDLQAVVEPVLAERRAARERELVERWREGSGNDRSTAGWGPTLTALSDARVET
                     LLAAPNGARAVWRCPSCGRAAAEPGECPLDGERMEEAPDGLGVALELALRNGGTVWIA
                     EGPELGPAEGIGAILRF"
     gene            complement(724..1923)
                     /locus_tag="VFO88_06485"
     CDS             complement(724..1923)
                     /locus_tag="VFO88_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET9545207.1"
                     /translation="MTYVFVALAAALAGGLAVWAVTRARVAALEATLHAAADMKDAFK
                     ALSAETVQTSLERLQAETARADAERRQAVEQMIRPIRESLERVDAQGRQLESARREAY
                     GSLTAQVKMLTENQERLRAETGNLVTALRNPAARGRWGEMQLRRVAEMAGMLAHCDFT
                     EQTTASVDDRRMRPDVVVRLPGNKHVVVDAKVPLEAYLLAVEATDEDARAAHLADHAR
                     QVRQHIQKLNQKAYWEQFQPSPEFVVLFIAGEAFFSAALQHDATLLEEMVGQKVIVAT
                     PTTLISLLKTVSLLWHEEKVAESAREVSALGRDVYQRLATMGEHFAKLGQRLEGAVGA
                     YNETVGSLERRVLPAARRLADHGAGGSKEIAIVDPVDRAPQLPQAPELAVERGEVAEL
                     PQARDAA"
     gene            1974..2894
                     /locus_tag="VFO88_06490"
     CDS             1974..2894
                     /locus_tag="VFO88_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET9545208.1"
                     /translation="MRDPVTVACAQIEPVVLDRERTLDRLAERTAEAAAAGAGLVVFP
                     EAFVPAYPSSVWARALAGWADPRAKAAFALLARESVEVPGPAADRLGEVAREHGVWLV
                     TGVTEIDPERPGTLYNTLLTHSPEGELALHHRKLVPTNHERLVWGPGDGSGLHAIETP
                     IGRIGGLICWENYMPLARFALYETGVEIYVASTADDSDAWQATLVHIARESRSFVVSP
                     CHFQRAAAYPDDFPLRAELEGVEIVGRGGSAILGPDGAYLAGPLYDEEGILYAELDPA
                     RLYEERQRFDPAGHYHRPDVLGLTVRRPDG"
BASE COUNT          373 a         1141 c         1136 g          406 t
ORIGIN      
        1 gggcgcgagg ccggccgctt ctactcgctc cgcggcgggc ggctcgagga gatcaccgac
       61 ctctccacgg agacgcccgg ccgccacgac cagggcggct ggtcgcaggc gcggttccag
      121 cggcacatcg acgagctcgc ggcaggccac ctgcgcgccg tggcggagga gatcgaccgc
      181 cgcgtccggc gggcgggcgg cgggatcgac gtcgtggtcg tcgcgccgga ggagagccgc
      241 gccgagctcg ccggctacct cacgcaggac gcggagcacg ccctggcggg ctgggcgctg
      301 gcggaggcgc acgcggggcc cacggatctc caggcggtcg tcgagcccgt tctcgcggag
      361 cgccgtgccg cccgggagcg cgagctcgtc gagcgctggc gcgagggaag cgggaacgac
      421 cgcagcacgg ccggctgggg gccgacgctc accgcgctct cggacgcccg cgtcgagacg
      481 ctgctcgccg ccccgaacgg cgcacgcgcg gtctggcgct gcccgtcctg cgggcgcgcc
      541 gccgccgagc cgggcgagtg cccgctcgac ggcgagcgga tggaggaggc gcccgacggc
      601 ctgggcgtcg ccctcgagct cgccctccgg aacggcggca cggtctggat cgccgagggc
      661 cctgagctcg gcccggccga gggaatcggc gcgatcctcc gcttctgagc cggccggccc
      721 gggctaggcc gcgtcgcgcg cctgcgggag ctcggccacc tcgccgcgct cgaccgcgag
      781 ctccggcgcc tgcgggagct gcggggcgcg gtcgaccggg tcgacgatcg cgatctcctt
      841 cgacccgccc gcaccgtgat cagcgaggcg ccgcgccgct gggaggacgc gccgctcgag
      901 cgacccgacc gtctcgttgt aggcgccgac cgcgccctcg agccgctggc cgagcttcgc
      961 gaagtgctcg cccatggtcg caaggcgctg gtagacgtcc cggccgagcg cgctcacctc
     1021 gcgggcgctc tcggcgacct tctcctcgtg ccagaggagc gacacggtct tcaggaggct
     1081 gatgagggtc gtcggcgtgg ccacgatcac cttctggccc accatctcct cgagcagcgt
     1141 cgcgtcatgc tggagcgcgg cgctgaagaa cgcctcgccc gcgatgaaga ggacgacgaa
     1201 ctccggcgac ggctggaact gctcccagta cgccttctgg ttcagcttct ggatgtgctg
     1261 gcgcacctgc cgcgcgtggt cggcgaggtg ggcggcccgt gcatcctcgt cggtcgcctc
     1321 gacggcgagg aggtacgcct cgagcggcac cttcgcgtcg acgacgacat gcttgttccc
     1381 cggcaggcgg acgacgacgt cggggcgcat gcgccggtcg tccacgctcg ccgtcgtctg
     1441 ctcggtgaag tcgcagtgcg cgagcatccc ggccatctcc gcgactcggc ggagctgcat
     1501 ctcgccccag cggccgcggg cggccgggtt gcggagcgcg gtgacgaggt tcccggtctc
     1561 ggcgcggagc cgttcctggt tctcggtgag catcttgacc tgcgccgtga gcgagccgta
     1621 ggcctcgcgg cgggcgctct cgagctgccg gccctgtgcg tcgacccgct cgagcgactc
     1681 gcgaatcggg cggatcatct gctcgaccgc ctggcgccgc tccgcgtcgg cgcgggccgt
     1741 ctcggcctgg aggcgctcca ggctcgtctg gacggtttcg gccgagagcg ccttgaacgc
     1801 gtccttcatg tcggcggccg cgtggagcgt cgcctcgagg gccgcgacgc gcgcgcgggt
     1861 gaccgcccag acggcgaggc ctccggcgag cgctgctgcg agggcgacga aaacgtaggt
     1921 catcggtcta cgaacgctag ggaccggccc ggacggcatg gaagaatggg cggatgcgcg
     1981 accccgtcac cgtcgcgtgc gcccagatcg agccggtcgt cctcgaccgg gagcgcacgc
     2041 tcgaccggct cgcggagcgc acggccgagg ccgcggcggc gggtgccggg ctcgtcgtgt
     2101 tcccggaggc gttcgtcccc gcgtatccgt cctccgtctg ggcccgcgcg ctcgccggct
     2161 gggcggatcc gcgcgccaag gccgcgttcg cgctcctcgc gcgcgagtcc gtggaggtgc
     2221 ccggcccggc cgccgaccgg ctcggcgagg tcgcgcgcga gcacggcgtc tggctcgtca
     2281 ccggcgtgac cgagatcgac cccgagcgcc cggggacgct ctacaacacg ctcctcacgc
     2341 actctcccga gggagagctc gcgctccacc accgcaagct cgtcccgacg aaccacgagc
     2401 ggctcgtctg gggcccgggc gacggctcgg ggctccacgc gatcgagacg ccgatcgggc
     2461 ggatcggcgg cctcatctgc tgggagaact acatgccgct cgcgcgcttc gcgctctacg
     2521 agacgggcgt cgagatctac gtcgcctcga ccgccgacga ctccgacgcg tggcaggcga
     2581 ccctcgtcca catcgcccgc gagtcgcgct cgttcgtcgt cagcccgtgc cacttccagc
     2641 gggcggccgc gtatcccgac gacttcccgc tccgcgcgga gctcgagggc gtcgagatcg
     2701 tcgggcgcgg cggcagcgcg atcctcggcc ccgacggcgc atatctcgcg gggccgctct
     2761 acgacgagga ggggatcctc tacgcggagc tcgacccggc gcgcctctac gaggagcggc
     2821 agcgcttcga tcccgccggg cactaccacc ggccggacgt gctggggctg acggttcggc
     2881 ggccggacgg ctgacgaacg cggtaccgtt aattcccccg tggcgaagaa ggatcgagcc
     2941 cccacaccgc ctcgcccagt ccaggcgccg aaggtccgga gcacgcgcga gccgcgcgat
     3001 ccgcggcgta cgcggctgct catcgcggcc gtggccggca tcgtgctcct cgccgc
//
LOCUS       DASTFC010000218         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1158228,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000218 DASTFC010000000
VERSION     DASTFC010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158228"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFO88_06495"
     CDS             <1..321
                     /locus_tag="VFO88_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9545209.1"
                     /translation="IFGPVVVAIPFADEKEAIALANDSKYGLVATVWSQDPARGHRVA
                     ARLKAGTVGINMPYSAFPGIPFGGYKQSGFGRETGLETLDLYTESKGVILQTGPKPVN
                     PFGL"
     gene            335..1498
                     /locus_tag="VFO88_06500"
     CDS             335..1498
                     /locus_tag="VFO88_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET9545210.1"
                     /translation="MERVLERLERLFALGGGTGANRPGLSRAEQEAHDLVAAWMEEDG
                     LAVSVDPVGNLVGRLPGTRSDLPEAWTGSHLDSVPDGGRYDGALGVVGGLEAVVQAAA
                     AGAHERTLAVIAFRDEEGSRFGRGLFGSRALCGRLATDDLEQCDAAGVSVREALAALG
                     LPSPDPAGCLERAPAAFVECHVEQGAVLAARGAPLGVATAIVGVARGWAEFSGLARHA
                     GTTPMDARDDALVAAAEFVLRLRDAAQAVDGAVATAGRIELEPGAVNVVPARARVSVD
                     VRAPDRGRLDAVLTAVPEAHVHVTEPVPLAPGPTDALRGAIADAGLPVEQLASGAGHD
                     AMVLAAAGVPTGLLFVRSLADGASHCPEEETSAEDVALAVEVLAGALRRLAGA"
     gene            complement(1495..1902)
                     /locus_tag="VFO88_06505"
     CDS             complement(1495..1902)
                     /locus_tag="VFO88_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HET9545211.1"
                     /translation="MLEITAGPLTFRARLEEELAPRTCAALRKLLPLEDKIVQARWSG
                     ESAWIPLGDLETGVGPENANSYPGPGELLLYPGGLSETEILFPYGATCFASKMGQLAG
                     NHFATIVEGNDGLAELGRLVMWEGAQDVRFAEA"
     gene            1928..>2290
                     /locus_tag="VFO88_06510"
     CDS             1928..>2290
                     /locus_tag="VFO88_06510"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET9545212.1"
                     /translation="MDPYLSDWLTILLRWLHVVAGIAWIGASFYFIYLDLSLRPPKRR
                     EDRQAGVSGEFWAVHGGGFYHSQKYRVAPATLPYPLHWFKWEAYTTWLSGFALFVVLY
                     YANADGFLVDPAVADIGEA"
BASE COUNT          261 a          839 c          868 g          322 t
ORIGIN      
        1 atcttcggcc cggtcgtcgt cgcgatcccg ttcgccgacg agaaggaggc gatcgcgctc
       61 gcgaacgact cgaagtacgg gctcgtggcg acggtctggt cgcaggatcc cgcgcgcggc
      121 caccgcgtcg ccgcccggct caaggcgggc acggtcggga tcaacatgcc gtacagcgcc
      181 ttcccgggca tcccgttcgg gggctacaag cagtccggct tcggccgcga gaccgggctc
      241 gagacgctcg acctctacac ggagtcgaag ggcgtcatcc tccagaccgg cccgaagccc
      301 gtcaacccgt tcggcctcta ggagccgcgc aggcatggag cgcgtgctcg agcggctcga
      361 gcggctgttc gcgctcggcg gcggcacggg cgcgaaccgg cccggcctct cgcgcgccga
      421 gcaggaggcg cacgacctcg tggcggcgtg gatggaggag gacgggctcg ccgtctcggt
      481 cgatccggtc gggaacctcg tgggccggct ccccgggacg cggtcggatc tcccggaggc
      541 ctggacgggc tcgcacctcg actcggttcc ggacggcggg cgctacgacg gggcgctcgg
      601 cgtcgtcggc gggctcgagg cggtcgtgca ggcggccgcc gcgggcgcgc acgagcggac
      661 gctcgcggtc atcgccttcc gcgacgagga gggcagccgc ttcgggcgcg ggctcttcgg
      721 cagccgggcg ctctgcgggc ggctggcgac ggacgacctg gagcagtgcg acgcggccgg
      781 cgtgagcgtg cgcgaggcgc tcgccgccct cggccttccc tcgcccgatc cggccgggtg
      841 tctcgagcgc gcgccggcgg cgttcgtcga gtgccacgtg gagcagggcg ccgtgctcgc
      901 cgcgcgcggg gctccgctcg gggtcgcgac cgcgatcgtc ggcgtcgcgc gcgggtgggc
      961 ggagttctcc ggcctcgccc ggcacgccgg gacgacgccg atggacgcgc gcgacgacgc
     1021 cctcgtcgcc gcggcggagt tcgtcctgcg cctgcgcgac gccgcccagg cggtcgacgg
     1081 tgcggttgcg accgccgggc ggatcgagct cgagcccggt gcggtgaacg tcgtcccggc
     1141 ccgggcgcgg gtgagcgtcg acgtgcgcgc gcccgaccgc ggccggctcg acgcggttct
     1201 caccgccgtc cccgaggccc acgtgcacgt cacggagccg gtgccgctcg cgcccggccc
     1261 gaccgacgcg ctccgcgggg cgatcgcgga cgcggggctc ccggtcgagc agctcgcctc
     1321 cggcgccggg cacgacgcga tggtgctcgc cgcggccggc gttccgaccg ggctcctctt
     1381 cgttcgcagc ctcgccgacg gcgcgagcca ctgcccggag gaggagacga gcgccgagga
     1441 cgtcgcgctc gcggtcgagg tgctcgcggg ggcgctccgc cggctggccg gcgcctaggc
     1501 ctccgcgaag cgaacgtcct gggcgccctc ccacatcacg aggcgcccga gctctgcgag
     1561 cccgtcgttg ccctcgacga tcgtggcgaa gtggttgccc gcgagctggc ccatcttgct
     1621 cgcgaagcag gtggcgccgt acgggaagag gatctccgtc tccgagagcc cgcccgggta
     1681 gagcaggagc tccccggggc cggggtagga gttcgcgttc tccggcccga cgccggtctc
     1741 gagatcgccg agcgggatcc atgccgactc cccgctccag cgcgcctgca cgatcttgtc
     1801 ctcgaggggg aggagcttcc ggagcgcggc gcacgttcgc ggcgcgagct cctcctcgag
     1861 ccgtgcgcgg aacgtgagcg ggcctgccgt gatctcgagc acgtgcgcag tctaaggtcc
     1921 cggcccggtg gatccgtatc tcagcgactg gctcacgatc ctcctgcgct ggctgcacgt
     1981 cgtggccggg atcgcgtgga tcggtgcctc gttctacttc atctacctcg acctctccct
     2041 gcgcccgccg aagcggaggg aggaccggca ggcgggcgtc tccggtgagt tctgggccgt
     2101 ccacggcggc ggcttctacc actcgcagaa gtaccgggtc gcgccggcga cgctgccgta
     2161 cccgctccac tggttcaagt gggaggcgta cacgacgtgg ctctccggct tcgcgctctt
     2221 cgtcgtcctc tactacgcga acgcggacgg gttcctcgtc gacccggccg tcgcggacat
     2281 cggcgaggcg
//
LOCUS       DASTFC010000219        10943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1092681,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000219 DASTFC010000000
VERSION     DASTFC010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092681"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="VFO88_06515"
     CDS             complement(<1..1643)
                     /locus_tag="VFO88_06515"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET9545213.1"
                     /translation="MEAFFQDHSVLFGLACAAVGIAWAVVLTARLLRLSPGSERMQEI
                     GRAIQEGASAYLRRQYLTIAVVAVVLFLGIGLWDELGWGTAFGFLIGAVFSAAAGFIG
                     MNVAVRANTRTAEAARGGVRPALAVAFSAGSVTGLLVVGLGLLGVAGYYGILTSWFDR
                     DAEQATRELIGLAFGGSLISVFARLGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAV
                     IADNVGDNVGDCAGMAADLFETYAVTAVAVMFLGALNFGDTDLVVYPLAIGGVAIIAS
                     VIGTFFARIGRSGSIMGALYKAVAVAVVLSAVGFIPVTMAFDTGDFSFAELYGCTLIG
                     LGVTFLLVGITEFYTGTRWRPVKGISAASTTGHATNIIEGLAIGMQATALPVIVIAAA
                     IVGANSLAGLYGVGVAVMAQLAMTGLIVALDAYGPVTDNAGGIAEMADLPEEVRGVTD
                     PLDAVGNTTKAVTKGYAIGSAGLAALILFDGFTTELGLEGISATFELDDAWVIAGLFI
                     GGLMPFLFSSLAMQAVGRVGGQVVEEVRRQFRENPGIMEG"
     gene            complement(2056..2358)
                     /locus_tag="VFO88_06520"
     CDS             complement(2056..2358)
                     /locus_tag="VFO88_06520"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HET9545214.1"
                     /translation="MENGKERAGHVGRRAYVVESYAPGPADASLSALTAQARLVASAA
                     LAAEGTPVRLLRSLLVPEDEVCFFVFEGVSAEAVWEVSRRAGLRAERVAEAVEVPH"
     gene            complement(2420..5191)
                     /locus_tag="VFO88_06525"
     CDS             complement(2420..5191)
                     /locus_tag="VFO88_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545215.1"
                     /translation="MRSVATSLVGRDEELESIEAFLARATEASSALVLAGEPGIGKTS
                     LWRHGLDAAEDAGVRTLAARPNEAEAAFAYAALTDVLATVREETAPLLPEPQRRALEI
                     ALLLEEPDAATERHGVALAFLGVLRMLAEDAPVLVAVDDVQWLDRASADVLAYAARRV
                     DDEPVGFLLARRVERDGAPSGLERAFRAERLETRTLRPLSLGAVHRLLHERLGRAFAR
                     PVLRRIHELSGGNPFYALELAEALEREAGRLDRDGPLPLTLEALVRERLVGLPRATRR
                     VLAAAAAGAQPTVALLERAGESDALASLAPAVEADVVAVENGRVRFTHPLLASGAYAL
                     AGVAERRDLHRILAGLADDPEERARHRALAAPGPDPEVADALDAAASQARRRGAPVVA
                     AELGERAVALTPPGRVHDRQRRLVETALFHFESGDSSRARSMLEQIAGELPFGGERAR
                     TLVALARVRSYDDDLRAATALFEQALAEAEDAQVRAAAHEGVSACLFRRRERHADGID
                     HGQAAFELARELGNRPLLVEALGSRMLHEAILGRPAAARTLEQALALRGEGTGERVLR
                     DPRFAAGVLRLWWDELERAHAEFEELAAAARATGDEASLPYVLFMLAQTECRLGAFDE
                     AAARAEEANELTVQSGQEWLEAYAHALRALADAYRGREDGARAAAARALALAGATGSA
                     PAEQFALTALGHLELSLGRPGDAWSHLEPVVVWAEREGADEPGAVPCLPDAVEALVEA
                     GRLDEAEEALGRLEVNARRLGRASAEAACARLEALLAAARGEREAAAAAFERALAAHE
                     HVPMPLERARTLIARGAFLRRGKRKREARESLEQAVALCEEVGAAVWAERARSELGRI
                     GGRAPSRGELTPAERRVAELVAEGRTNREVAAALFLSDRTVEGHLSRVYMKLGVRSRA
                     ELARRFAST"
     gene            complement(5277..5747)
                     /locus_tag="VFO88_06530"
     CDS             complement(5277..5747)
                     /locus_tag="VFO88_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545216.1"
                     /translation="MARRTRIGAVAAVLAAIGAATALATPGGGITDVVNFVSADSGKR
                     IVAHDAGIHLVTTRPTTAFMQTATLQPGGTSGWHGHRGLVVLAVESGALTRYDGHCRA
                     KQFPAGSYFVEGGAHPVLIRNEGAVPARFYITYLMPANDPTRRIDLPNPGCPVE"
     gene            complement(5830..6621)
                     /locus_tag="VFO88_06535"
     CDS             complement(5830..6621)
                     /locus_tag="VFO88_06535"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005876.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET9545217.1"
                     /translation="MPGQAEIGVFGGSGFYSFLEDVETVEVETPYGRPSGPFTIGEIG
                     GKRVAFLPRHGPSHELPPAQIPYRANVWAMKELGVRRIIGPNASGSLKAEIGLGEFVV
                     TDQFVDRTSGREDTFYEGPETTHVSAADPYCPELRGLLVETARELGIPVRDGGTVVVI
                     QGPRFSTRAESKWFQDMGWDTINMTQYPEGYLARELELCYANISMVTDHDVGVAGAEP
                     VSHEQVVTVFEENNEKLRELLFAVIPKIGPQPEDVCATALQGARI"
     gene            6602..7432
                     /locus_tag="VFO88_06540"
     CDS             6602..7432
                     /locus_tag="VFO88_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9545218.1"
                     /translation="MSAWPGTAATLSDRARRTGRRLARTEGLAYTDEGGGRPVVLLHG
                     LTCHLGYWLRVTPLLDGLRVVALDLRGHGLSEHRGSYRYADYEADLLALLDELGLDRA
                     FVAGHSLGGYVALRAAVGSDRIAGVLALDVKSDWTEADATLAERARDAEQRVESSREA
                     LVDRLARGLAPADLGADELEALAERALEPAGGGWRFRWDRRVLATEPVAPFAFLGKVR
                     CPVRVIAGAESPVMPPEAARLFAAAIPGATLELLDGIGHHVELEAPEVVAERIRLLAA
                     "
     gene            complement(7429..7752)
                     /locus_tag="VFO88_06545"
     CDS             complement(7429..7752)
                     /locus_tag="VFO88_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545219.1"
                     /translation="MSEEQERLAAELAEELRKLRVEDVLVNTLLTVSSVGYRRLGLTE
                     DTREDRDLEQTRLAIETMRALTPVLERVVPPELVADFTSSVANLQLAYAKAASEEPAA
                     PDAEG"
     gene            complement(7745..8899)
                     /locus_tag="VFO88_06550"
     CDS             complement(7745..8899)
                     /locus_tag="VFO88_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HET9545220.1"
                     /translation="MMAPPNPGEELRLARERFEDADDFTLAVEEEFALLDPETLDLVN
                     RFEELEAASKGTELDGHLVGELIASEVEVRTGRCETFPEAAAKVTERRAQLLRLAEAH
                     GVGLCSAGTHPWSPWQDQRIIDTPHYRLNDELLRYVVWRNTTFGLHVHTAIRGADRAV
                     RVAGALRAFLPDLLALSASSPFTENVFSGLHSARSQLFTKMFPRCGIPDAYDGWDDYE
                     RYVRFLYDTGSITKHTQIWWSVRPHLAFPTVEVRICDGQPQLGESLALAALLHALTVR
                     IARALDEGEPLPDCPHALLEENFWRAIRYGLAGELIDLRAYPSPRVRPARAALEELVE
                     WVMPAAEEVGAAPYLAVPAANPAERQIARHEAGGTLREIYAAEVLEPARV"
     gene            9016..>10943
                     /locus_tag="VFO88_06555"
     CDS             9016..>10943
                     /locus_tag="VFO88_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9545221.1"
                     /translation="MADVFISYSRADGDFVRELHGFLTGHGKDVWVDWEDIPPAAAWE
                     EDIDDSIDEAESFVFVVSGNSLASRYSLDELHHAQDRGKRIVPIACDGADPEAAPEGL
                     RQLNWIWCRPDDDRAAAFERVLEALDTDLGWAEVHTRLLVRAVEWDERRDSSLLLRGR
                     DLEDALQATAANAGKEPVPTELQREYLLASRRASSRRQRLVLGSVSLALAVSVGLGLV
                     ALVQRNLANDRARVARSQALAAQAVAALGSEPAAALADAVRAMETEPTPEARVALRRA
                     LLATPVAYSVGAEGRTRGPARFDSVAFGAGGRTLVGLGPDGTLRIRESATGREIARTR
                     AAVFAPEGRLAVAVSRQDAQVLDLRTGAVVATRRAPAGGQPVGAGFSRGVPRAAYAVG
                     GAVTLVRLPAGRAVTLSVRPGPLLRVRFAADGGRAVTADGRRARVWDSASGRLLATLG
                     TATSAAISRDGRFVATVGTFGDATLWRADDGTRLAALGRAANVVFSRDGRLVLTVAED
                     GSAAVWRTATGRQAAVLPGFGSLVTGVAGSTVFSDFSPGAAFSGDGRLVAVANPDGNV
                     RIWDVAARKQVGAVGTGWTNAVAFAPRGGLLAAAAWNGELVVARTPPSVPLRTGARPS
                     TCVPTFDPIMTGDGRHVVA"
BASE COUNT         1444 a         4071 c         4043 g         1385 t
ORIGIN      
        1 ccctccatga tccccgggtt ctcgcggaac tggcggcgca cctcctcgac cacctgtccg
       61 ccgacgcggc ccacggcctg catggcgagc gaggagaaga ggaacggcat gagcccgccg
      121 atgaagagcc cggcgatcac ccaggcgtcg tccagctcga aggtcgccga gatcccctcg
      181 agcccgagct ccgtcgtaaa gccgtcgaag aggatgagcg ccgcgagccc ggcggagccg
      241 atcgcgtagc ccttcgtgac cgccttcgtc gtgttgccga ctgcgtcgag cgggtcggtg
      301 acgccgcgca cctcctccgg caggtcggcc atctcggcga tcccgccggc gttgtccgtg
      361 acggggccgt aggcgtcgag cgcgacgatg aggcccgtca tcgcgagctg cgccatgacc
      421 gcgactccga cgccgtagag cccggcgagc gagttcgcgc cgacgatcgc ggccgcgatc
      481 acgatcacgg gcagcgcggt cgcctgcatg ccgatcgcga gcccctcgat gatgttcgtc
      541 gcgtggcccg tcgtggacgc ggccgagatg cccttcacag ggcgccagcg tgtgcccgtg
      601 tagaactccg tgatgccgac gagcaggaac gtgacgccga ggccgatcag ggtgcagccg
      661 tagagctcgg cgaaggagaa gtcgcccgtg tcgaacgcca tcgtgaccgg gatgaacccg
      721 accgccgaga gcacgaccgc gaccgcgacc gccttgtaga gcgcgcccat gatcgagccg
      781 gagcggccga tccgcgcgaa gaacgtcccg atgacggagg cgatgatcgc gacgccgccg
      841 atcgcgagcg ggtagacgac gagatccgtg tccccgaagt tgagcgcgcc gaggaacatg
      901 accgcgacgg ccgtcacggc gtacgtctcg aagaggtctg ccgccatccc ggcgcagtcg
      961 cccacgttgt cgccgacgtt gtccgcgatc acggcggggt tgcgcggatc gtcctcgggg
     1021 atgcccgcct cgaccttccc gacgaggtcg gcgccgacgt cggcggcctt cgtgtagatg
     1081 ccgccgccga gccgcgcgaa gaccgagatg agcgagccgc cgaacgcgag cccgatcagc
     1141 tcgcgcgtcg cctgctccgc gtcgcggtcg aaccagctcg tgaggatgcc gtagtagccg
     1201 gccaccccga gcagcccgag gccgacgacg aggaggcccg tgaccgagcc ggccgagaac
     1261 gcgaccgcga gcgcgggcct gacgccgccg cgggccgcct ccgccgtgcg cgtgttcgcg
     1321 cggacggcga cgttcatccc gatgaacccg gccgcggccg agaagaccgc gccgatcagg
     1381 aagccgaacg ccgtccccca cccgagctcg tcccagaggc cgatcccgag gaagaggacg
     1441 accgcgacga ccgcgatcgt caggtactgg cgccggaggt acgccgaggc gccctcctgg
     1501 atggcgcgcc cgatctcctg catccgctcc gagccgggcg acaggcgcag caggcgcgcc
     1561 gtgaggacga cggcccaggc gattcccacg gccgcgcagg ccagcccgaa gagcacactg
     1621 tgatcctgga aaaacgcttc cacttgctcc cgcctcgtcg agttacgaag tcggcgtgtc
     1681 cgtcacgtgc ggggctcccc gatccctgat cgcacgccgg tttcgccggg cggcattcta
     1741 tgcacaaagt ccggccggac gcgctcgcta cacgggcggg ggcgcagccg tcgcgcgccc
     1801 tgacagaaga cggcgagcga gaagccgacg tcctggacgg cgttgctccc gaagacgtcc
     1861 tagccctcca cgctggacga gcacgtccct cggatcggtg ctcgcgtgcg gatgctgggc
     1921 ggcgaggaac tcaccgtcgg cgtcccggcg ggaccggaag gaccgggctg ctcctgcggc
     1981 ccccgcgaat gcgagacatg cgaccgtcac ggcagggcga tggcaacgga atccgtcgac
     2041 ctgcacggtt ctctttcagt gcggcacctc gaccgcctcg gcgacgcgct cggcccggag
     2101 gccggcgcgg cggctcactt cccatacggc ctcggccgac accccctcga agacgaagaa
     2161 gcacacctcg tcctccggga cgaggagtga gcgcagcaag cggacgggcg tgccctcggc
     2221 cgcgagcgcc gccgaggcga ccagcctcgc ctgcgcggtg agggcgctga gcgacgcgtc
     2281 ggccggcccg ggcgcgtagg actcgacgac gtacgcacgc cggcccacgt gcccggccct
     2341 ctccttcccg ttctccatac cgcccgacgg tagggccgcg ccgcgcgctc ggaaacgtgg
     2401 aaaccccctg tctttggccc taggtggagg cgaagcgacg cgcgagctcg gcacgtgagc
     2461 gcacgccgag cttcatgtag acgcgggaga ggtggccctc caccgtccgg tcgctcagga
     2521 agagcgcggc ggccacctcg cggttcgtcc ggccctccgc gacgagctcg gcgacgcgcc
     2581 gctcggcggg ggtgagctcg ccgcgcgagg gagcgcgccc gccgatccgc cccagctcgc
     2641 tccgggcgcg ctccgcccac acggcggcgc cgacctcctc gcagagcgcg accgcctgct
     2701 cgagcgactc gcgcgcctcg cgcttgcgct tcccgcggcg cagaaacgcc ccgcgcgcga
     2761 tgagcgtgcg cgcacgctcg agcggcatcg ggacgtgctc atgcgcagcg agcgcgcgtt
     2821 cgaatgccgc ggcggcggcc tccctctcgc cgcgcgcggc ggcgagcagc gcctcgagcc
     2881 gggcacaggc ggcctccgcg gaggcgcgcc cgagccgccg cgcgttcacc tcgagccggc
     2941 cgagcgcctc ctccgcctcg tcgaggcggc cggcttcgac gagcgcctcg accgcatccg
     3001 ggaggcacgg caccgcgccc ggctcgtcgg cgccctcccg ctccgcccag acgacgacgg
     3061 gctcgaggtg cgaccacgcg tcgcccggcc ggccgaggga gagctcgagg tggccgagcg
     3121 ccgtcaaggc gaactgctcc gccggcgcgg agcccgtcgc ccccgcgagc gccagcgcgc
     3181 gggcggccgc agcacgggcc ccgtcctccc gtcctcggta cgcgtccgcg agcgcgcgca
     3241 gggcgtgcgc gtaggcctcg agccactcct gtccggactg cacggtcaac tcgttcgcct
     3301 cctcggcccg cgcggcggcc tcgtcgaacg cgccgagcct gcactcggtc tgcgcgagca
     3361 tgaagaggac gtagggaaga gaggcctcgt cgcccgtcgc gcgggcggcc gccgcgagct
     3421 cctcgaactc ggcgtgcgcg cgctcgagct cgtcccacca aagacgcagg acgcccgcgg
     3481 cgaaacgcgg atcccgcagg actcgctctc cggttccctc cccgcggagc gccagcgcct
     3541 gctcgagcgt gcgcgcagcc gccggccggc ccaggatcgc ctcgtggagc atgcgggacc
     3601 cgagcgcctc gacgaggagc ggccggttcc cgagctcgcg cgcgagctcg aaggccgcct
     3661 gcccgtggtc gatgccgtcc gcgtgacgct cgcggcggcg gaagaggcag gcggacacgc
     3721 cctcgtgcgc ggccgctcgc acctgcgcgt cctccgcctc cgcgagcgcc tgctcgaaga
     3781 gcgcagtcgc cgcgcgcagg tcgtcgtcgt aggaacggac gcgcgcgagg gcgacgagcg
     3841 tgcgcgcgcg ctccccgccg aaaggaagct cgccggcgat ctgctcgagc atcgaccgcg
     3901 cgcgcgagga gtcgcccgac tcgaagtgga agagcgcagt ctccacgagc cggcgttgcc
     3961 ggtcgtgtac gcgcccgggc ggggtgagcg cgacggcgcg ctcgccgagc tcggccgcga
     4021 cgaccggcgc gccgcgtcgg cgtgcctgac tcgcggcggc gtcgagcgcg tccgcgacct
     4081 cgggatcggg gcccggtgcg gcgagcgccc ggtggcgcgc ccgttcctcg gggtcgtcgg
     4141 cgagcccggc gagaatccgg tggagatcgc ggcgctcggc gacgcccgcc agcgcgtagg
     4201 cacccgacgc gagcagcgga tgcgtgaacc tgacgcgccc gttctcgacg gcgacgacgt
     4261 ctgcctcgac ggccggagcg agggatgcga gcgcgtccga ctcgccggcc cgctcgagca
     4321 gggccaccgt cggctgtgcg ccggcggcgg ccgcggcgag cacgcgccgg gtcgcgcgcg
     4381 ggagccctac gagccgctcg cgcacgagcg cctcgagcgt gagcgggagc gggccgtccc
     4441 ggtcgagccg ccccgcctct cgctcgagcg cctcggccag ctcgagcgca tagaacgggt
     4501 tgccgccgga gagctcgtgg atcctgcgca ggaccggccg agcgaatgcc cgcccaagcc
     4561 gctcgtgcaa caggcggtga acggcgccga ggctgagcgg gcggagcgtg cgcgtctcga
     4621 gccgttcggc ccgaaacgcc cgctcgaggc cggatggcgc gccgtcgcgc tcgacccggc
     4681 gcgcgaggag gaagccgacc ggctcgtcgt caacccgccg cgccgcgtac gcgaggacgt
     4741 ctgccgaggc gcggtcgagc cactgcacat cgtccacggc gacgagcacg ggcgcatcct
     4801 cggcgagcat ccgcagcacg ccgaggaacg cgagcgcgac cccgtgccgc tccgtggcgg
     4861 cgtcgggctc ctcgaggagc agcgcgatct cgagcgctcg gcgctgtggc tctggcagaa
     4921 gaggggccgt ctcctcgcgg acggtggcga ggacgtcggt gagcgccgcg tacgcgaatg
     4981 ccgcctccgc ctcgttcggc cgggccgcga gcgtccgcac gccggcgtct tccgctgcgt
     5041 cgagcccgtg gcgccagagg gacgtcttcc cgatccccgg ctccccggcg aggacgagcg
     5101 cgctcgaggc ctcggtggcg cgcgcgagaa acgcctcgat gctctcgagc tcctcgtcgc
     5161 gcccgacgag cgaggttgcg acacttcgca caagaatgtg atatcacccc gccccacccg
     5221 tggaaagcgg gggagcgcgc ggcccgccgc ccgggccgcg cgcccacgcc acgcgcctac
     5281 tcgaccgggc agcccgggtt cgggaggtcg atccgccggg tcggatcgtt cgccggcatg
     5341 aggtacgtga tgtagaaccg cgccggtacg gcgccctcgt tacggatgag caccgggtgc
     5401 gcgccgccct cgacgaagta cgagccggcc gggaactgct tcgcccggca gtgcccgtcg
     5461 tagcgcgtga gcgcgcccga ctcgaccgcg aggacgacga gtcccctgtg cccgtgccac
     5521 cccgacgtcc cgcccggctg gagggtggcc gtctgcatga acgcggtcgt cgggcgggtc
     5581 gtgacgaggt ggatccccgc gtcgtgcgcg acgatccgct tgcccgaatc cgccgagacg
     5641 aagttcacca cgtccgtgat gccgccgccc ggcgtcgcga gcgcggtcgc cgccccgatt
     5701 gcggcgagca ccgccgcgac cgctccgatc ctcgttctcc gtgccatgtc gcctccttca
     5761 gttcgacccg cccgacgcta cggccggacg gcggaggcgg aaacgtggaa accccccgac
     5821 tttgccccgc tagatgcggg cgccctggag ggctgtcgcg cagacgtcct cgggctgcgg
     5881 gccgatcttc gggatgacgg cgaagaggag ctcgcggagc ttctcgttgt tctcctcgaa
     5941 gacggtcacg acctgctcgt gcgagaccgg ctcggcgccc gcgacgccga cgtcgtggtc
     6001 ggtgaccatc gagatgttcg cgtagcagag ctcgagctcg cgcgcgaggt agccctccgg
     6061 gtactgggtc atgttgatcg tgtcccagcc catgtcctgg aaccacttgg actccgcgcg
     6121 cgtcgagaag cgcgggccct ggatcacgac gaccgtcccg ccgtcgcgga cggggatccc
     6181 gagctcgcgg gccgtctcga cgagcaggcc gcggagctcc gggcagtacg ggtcggccgc
     6241 ggagacgtgc gtcgtctccg gcccctcgta gaacgtgtcc tcgcggccgc tcgtccggtc
     6301 gacgaactgg tcggtgacga cgaactcgcc gagcccgatc tccgccttga gcgagcccga
     6361 tgcgttcggg ccgatgatcc gccggacgcc gagctccttc atcgcccaga cgttggcccg
     6421 gtacgggatc tgcgccggcg gcagctcgtg gctaggcccg tgccgtggga ggaacgcgac
     6481 gcgcttgccg ccgatctccc cgatcgtgaa cgggccggac ggcctcccgt agggcgtctc
     6541 cacctcgacc gtctcgacgt cctcgaggaa cgagtagaag ccggagcctc cgaagacgcc
     6601 gatctccgcc tggccgggca cggcggcgac cctatccgat cgcgcgcggc gcacgggtcg
     6661 tagactcgcg cgcacggaag gactcgcgta caccgacgag ggcggcgggc ggcccgtcgt
     6721 cctcctccac gggctcacgt gccatctcgg ctactggctt cgcgtgacgc cgctcctcga
     6781 cgggcttcgc gtcgtcgcgc tcgacctccg cggccacggg ctgagcgagc accggggctc
     6841 gtaccgctac gcggactacg aggccgacct cctcgccctc ctagacgagc tcgggctcga
     6901 ccgggcgttc gtagcaggtc actcgctcgg cgggtacgtc gcgctgcgtg ccgcagtcgg
     6961 aagcgaccgg atcgccggcg tcctcgcgct cgacgtgaag agcgactgga ccgaggcgga
     7021 tgcgacgctc gccgagcgtg cgcgcgacgc cgagcagcgc gtcgagtcga gccgtgaggc
     7081 gctcgtcgac cggctcgcgc gcgggctcgc tccggccgac ctcggcgcgg acgagcttga
     7141 ggcgctcgcc gagcgcgcgc tcgagccggc gggcggcggc tggcgcttcc gctgggaccg
     7201 ccgcgtgctc gcgaccgagc cggtcgcccc gttcgcgttc ctcggcaagg tgcgctgccc
     7261 cgtgcgcgtc atcgccggcg ccgagagccc ggtcatgccg cccgaggcgg cgcgcctctt
     7321 cgccgcggcg attcccggag cgacgctcga gctcctcgac gggatcggcc accacgtcga
     7381 gctcgaggcg ccggaggtcg tcgccgagcg gatccggctg ctcgcggcct agccctcggc
     7441 gtccggcgcg gccggctcct ccgaggccgc cttggcgtag gcgagctgga ggttcgcgac
     7501 cgacgaggtg aagtcggcca cgagctcggg cgggacgacc cgctcgagca cgggcgtgag
     7561 cgcgcgcatc gtctcgatcg cgaggcgggt ctgctcgagg tcgcggtcct cgcgggtgtc
     7621 ctcggtcagc ccgagccgcc ggtagccgac cgaggagacc gtgaggagcg tgttcacgag
     7681 gacgtcctcg acgcggagct tgcgaagctc ctcggcgagc tcggcggcga ggcgctcctg
     7741 ctcctcagac acgggcgggc tccaggacct cggcggcgta gatctccctg agcgtcccgc
     7801 ccgcctcgtg gcgggcgatc tggcgctcgg ccggattcgc ggcgggcacg gcgaggtagg
     7861 gcgctgcgcc gacctcctcg gcggcgggca tcacccactc gacgagctcc tcgagcgccg
     7921 cgcgcgccgg ccgcacgcgc ggggacgggt aggcgcgcag gtcgatcagc tcgccggcga
     7981 gcccgtagcg gatcgcccgc cagaagttct cctcgaggag cgcgtgcggg cagtccggga
     8041 gcggctcgcc ctcgtcgagc gcgcgggcga tccgcacggt cagcgcgtgg aggagcgccg
     8101 cgagcgcgag cgactcgccg agctgcggct ggccgtcgca gatccggacc tcgacggtcg
     8161 ggaacgcgag gtgggggcgg acgctccacc agatctgcgt gtgcttcgtg atcgagccgg
     8221 tgtcgtagag gaagcggacg tagcgctcgt agtcgtccca cccgtcgtac gcgtcgggga
     8281 tcccgcagcg cgggaacatc ttcgtgaaga gctgcgagcg cgcggagtgc agcccggaga
     8341 agacgttctc cgtgaacggc gagctcgccg agagggcgag gaggtcgggc aggaaggcgc
     8401 gcagcgcgcc cgcgacacgg accgcgcggt cggccccgcg gatcgccgtg tggacgtgca
     8461 ggccgaaggt ggtgttgcgc cagacgacgt agcggaggag ctcgtcgttc aggcggtagt
     8521 gcggcgtgtc gatgatgcgc tggtcctgcc acgggctcca ggggtgcgtg cccgcggagc
     8581 agagcccgac cccgtgcgcc tccgcgagcc gcaggagctg cgcgcgccgc tcggtgacct
     8641 tcgcggccgc ctcggggaac gtctcgcagc gtccggtgcg cacctcgacc tcggaggcga
     8701 tcagctcgcc gacgaggtgc ccgtcgagct ccgtcccctt cgaggccgcc tcgagctcct
     8761 cgaagcggtt cacgaggtcg agcgtctcgg ggtcgaggag cgcgaactcc tcctcgacgg
     8821 cgagcgtgaa gtcgtctgcg tcctcgaacc gctcccgggc gagacgcagc tcctcgcccg
     8881 gattcggcgg ggccatcatg cgggcgaagg tatcaagaac cgctttcgta aatgaagcgt
     8941 tgccgtacga ccgcgttcgg tctacattcg gcggcgtggt cgagccgcgt cgaccgtccc
     9001 ggaagccgag gtgaggtggc agacgtcttc atctcgtact cgcgcgcgga cggcgacttc
     9061 gtgcgcgagc tgcacggctt cctgaccggg cacggcaagg acgtctgggt cgactgggag
     9121 gacatcccgc ccgcggccgc gtgggaggag gacatcgacg actcgatcga cgaggccgag
     9181 agcttcgtct tcgtcgtcag cggcaactcc ctcgcgtcgc gctactccct cgacgagctg
     9241 caccacgcgc aggaccgcgg gaagcggatc gtccccatcg cgtgcgacgg cgccgacccg
     9301 gaggcggcgc ccgaggggct tcgccagctc aactggatct ggtgccgccc ggatgacgac
     9361 cgcgccgcgg cgttcgagcg cgtcctcgag gcgctcgaca cggacctcgg ttgggcggag
     9421 gtgcacacgc ggctgctcgt ccgcgccgtc gagtgggacg agcggcgcga ctcgagcctc
     9481 ctcctccgcg gccgcgacct cgaggacgcg ctccaggcga ccgccgcgaa cgccgggaag
     9541 gagccggtgc cgacggagct ccagcgcgag tacctgctcg cgagccggcg tgcctcgagc
     9601 cggaggcagc ggctcgtcct cgggagcgtc tcactcgcgc tcgcggtctc cgtcgggctc
     9661 ggcctcgtgg ccctcgtcca gcggaacctc gcgaacgacc gcgcccgggt cgcgcgctcg
     9721 caggccctcg cggcgcaggc cgtcgcggcg ctcggctccg agcccgccgc cgccctggcc
     9781 gacgccgtcc gggcgatgga gaccgagccg acgcccgagg cgcgggtcgc cctgcggcgc
     9841 gcgctcctcg ccacgcccgt cgcgtactcg gtcggcgcgg agggccggac gcgcgggccc
     9901 gcgaggttcg actcggtcgc gttcggcgcc ggcggccgga cgctcgtcgg gctcggcccg
     9961 gacggcacgc tgcggatccg ggagagcgca accggccggg agatcgcgcg gacgcgggcg
    10021 gcggtgttcg cgccggaggg ccggctcgcc gtcgccgtct cccggcagga cgcccaggtc
    10081 ctcgatctcc ggaccggagc cgtcgtcgcc acgaggcggg ctcccgccgg cgggcagccg
    10141 gtcggagccg gcttctcccg cggcgtgccg cgcgccgctt acgccgtcgg gggagccgtc
    10201 acgctcgtgc gcctgccggc gggccgtgcg gtcacgctct ccgtccggcc cgggccgctc
    10261 ctgcgcgtgc gcttcgcggc ggacggaggc cgcgccgtca ccgcggacgg ccggcgcgcg
    10321 cgcgtctggg actcggcctc ggggcgcctg ctcgcgacgc tcggcaccgc gacctccgcg
    10381 gcgatcagcc gcgacggccg gttcgtagcg acggtcggga cgttcgggga cgcgaccctg
    10441 tggcgcgcgg acgacggcac gcggctcgcg gcgctcggcc gcgcggcgaa cgtcgtcttc
    10501 agccgggacg gccggctcgt cctcacggtc gccgaagacg gaagcgcggc cgtctggcga
    10561 acggcgaccg ggaggcaggc tgccgtgctc ccgggcttcg gcagcctcgt gaccggcgtc
    10621 gcgggctcga cggtgttcag cgacttctcg cccggcgcgg cgttcagcgg cgacggccgg
    10681 ctcgtcgcgg tcgcgaaccc cgacgggaac gtcaggatct gggacgtcgc cgcccggaag
    10741 caggtcgggg ccgtcggcac cgggtggacg aacgcggtcg ccttcgcgcc gcgcggcgga
    10801 ctgctcgccg cggcggcctg gaacggcgag ctcgtcgtgg cgcgcacgcc cccgagcgtc
    10861 ccgctccgca caggcgcccg gccgagcacc tgcgtcccca ccttcgaccc gatcatgacc
    10921 ggcgacggcc gccacgtcgt cgc
//
LOCUS       DASTFC010000220         9030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_416819,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000220 DASTFC010000000
VERSION     DASTFC010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416819"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFO88_06560"
     CDS             <1..507
                     /locus_tag="VFO88_06560"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9545222.1"
                     /translation="VLLDRDGTLVVDVPYNGDPRLVEPVPGAREALDRLREAGIPLGI
                     VSNQSGVGRGLLTLDQVEAVNRRVEQLLGPFDAWAVCPHAPEDGCECRKPRGGLVRQA
                     AEELGADPARCVLVGDIGADVEAARAAGARAVLVPTSLTRREEIEAAPEVAPDLLTAV
                     ELVLGGAR"
     gene            504..1553
                     /locus_tag="VFO88_06565"
     CDS             504..1553
                     /locus_tag="VFO88_06565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9545223.1"
                     /translation="MTHVLVARQDSAGDVLVTGPAIRAVAAGADQVTLVCGRRGRAAA
                     ELLPGVDALVQHTAPWIDPEPEPLRRAPTLELVEELAALELDEAIVFTSFHQSPLPLA
                     LLLRLAGVPRVSAISEDYPGSLLDVRHRPSDELHEVERALSLAAAAGYELPDGDDGRL
                     AVDRPGTEPPVDEPYVVVHPGTSAPARAWPPERHAELVAALAGEGRRVVVTGAPDERE
                     LTELVARDGGLDLGGATSLAELADVLAHADAVVVGNTGPAHLAAAVGTPVVSLFAPTV
                     PAGRWRPWGVPLELLGDQDAPCAGTRATVCPVPGHPCLCSVTPRDVVAALERLRGVRQ
                     AAIGHARPSMVEAAS"
     gene            1550..3418
                     /locus_tag="VFO88_06570"
     CDS             1550..3418
                     /locus_tag="VFO88_06570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9545224.1"
                     /translation="MKVALVSEHASPLAVLGGVDAGGQNVHVAALAGELARRDVEVVV
                     HTRRDDPGLPEHVELCPGVVVHHVDAGPPSAIPKDDLLPYMDDFAHVLHDAWADAPPD
                     LVHSHFWMSGRASIQAARPLGIPLVHTFHALGVVKKRHQGALDTSPPGRVAAEERIAR
                     RCDHVIATATDEVFELLRMGVDVRRVSVVPCGVDLELFRPVGPVEERTPRRARIVVVS
                     RLVQRKGVGNVISALAELPGVELVVAGGPERAELGSDPEACRLAALADELGLADRVDF
                     RGRLEREDLPALLRSADAAVCVPWYEPFGIVPLEAMACGVPVVASAVGGLLDTVVDGE
                     TGVLVPPRSPERLAEVLRELIADGAARERMGRAGVERTRARYSWGLVADQTLRVYESL
                     PRRPARRPLQLVGNGNGNGDGSHSLRRSSAEHLAASVRGLATLVAETQRIESWGRSLA
                     RTLLSGGRLLAVGNGGSAAQAQHLTGELVGRYSCERPALSAIPLHGDTSTLTAIENDY
                     GREEAFARQVRAHGRPGDVLFCLSTSGRSPNVLAAARAAAEGGLTVWGLTGPAPNPLA
                     DLCDDVIPVDAPTPANVQELHLVALHLLCAAVDEEVETSAAHAATRHVEELHHGIA"
     gene            3405..4097
                     /locus_tag="VFO88_06575"
     CDS             3405..4097
                     /locus_tag="VFO88_06575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9545225.1"
                     /translation="MASRDLGTVVVTGGASGLGAAIARAIAQNGGTPVVLDRVEPPYD
                     VEHVVVDLADRAAAEDAVRGSAERHGGLDAVVAAAGTDSCGKLDDVPRDDWERVVLVN
                     LLGTAAVVRAALPYLEASEGRVVTVASTLGLRALSDATAYCASKFGVVGFTRALTAET
                     AGRVGVTLLLPGGMQTNFFDGRPDEYKPGPDAQLNRPEDVADAVLFALTRPAGCEVRE
                     LLVAPARESSWP"
     gene            4088..5104
                     /locus_tag="VFO88_06580"
     CDS             4088..5104
                     /locus_tag="VFO88_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9545226.1"
                     /translation="MAVAVDRLDPPLPQELQVEVTGACNLRCRMCLVSYRPALGKTSG
                     AMDLASFTELLDSLPDLRKVTMQGLGEPLLAPDFFAMLELLASRGIAMGFNTNGTLLT
                     RERAERLVDLGVDWLHVSLDGATAPTYEDVRRRSQFELVRRNVAGLMEAMRERGAERP
                     EVELVFVAMRRNVHELPALVRLAADLRVPTVWVQNLSHSFSDTDPSGDYREIREYAEA
                     EALWAERNEEAELLFAEAERVAAELGVRLRLPRLEDPARAREPGQPGCGWPFDSAYVT
                     HDGKVQPCCAVMGADRAVLGDVKADGFERVWRNDAYRDFRAGLLGDEPPAVCRGCSMY
                     RHVF"
     gene            complement(5115..5432)
                     /locus_tag="VFO88_06585"
     CDS             complement(5115..5432)
                     /locus_tag="VFO88_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545227.1"
                     /translation="MQEAELAFAAEIDGRPGIVVNFGVYAGREATIAEVERLGTELLE
                     RVSSLEVVCERRESFGPDRRAELYQVRVELPGHDPAEASALVPAVEAWAQDCISERRL
                     ITP"
     gene            complement(5441..6193)
                     /locus_tag="VFO88_06590"
     CDS             complement(5441..6193)
                     /locus_tag="VFO88_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9545228.1"
                     /translation="MRLFLHELAAQQRLYWRSREAAFFTFVMPLVFLVLIGSVYGSGE
                     VDGIRGSTYLLAGLLGYSVIATAFAGLAITLVVRRESGVLKRVRGTPLPTATFLAAVI
                     GSTLVVIALGSIAQLLVGRFVVDAGWPSRPGELAVALLVGAACFAALGLAITGLIRSA
                     EGSSALVNAIYLPLVFISGVFFSVDSLPGFLQAVAEISPLTYLLRLVRECVVAGGEGL
                     GGALEAIAVLAGWGLAGLVLAVRMFRWEPREA"
     gene            complement(6190..7038)
                     /locus_tag="VFO88_06595"
     CDS             complement(6190..7038)
                     /locus_tag="VFO88_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9545229.1"
                     /translation="MEHAIEIRGLRKSYGPREVLHGLDLDVPAGEVFALLGPNGAGKT
                     TTVEILEGYRERDGGEARVLGVDPGRATGDFRARIGIVLQSSAVYPTLSVGELLELFA
                     GYYPSPREPGEVVRLVGLEEKRDARVRTLSGGQLRRLDLGLALVGDPELVFLDEPTTG
                     FDPAARRRAWETIRGLRALGKTILLTTHYMEEAQTLADRLAILRDGRIVATGSPRDLL
                     DGHATVEIRYRSGAEEVVLETEEPTRVLNELTADALAAGRELDSIQVRRRTLEDLYLE
                     LTRDGE"
     gene            complement(7343..8221)
                     /locus_tag="VFO88_06600"
     CDS             complement(7343..8221)
                     /locus_tag="VFO88_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9545230.1"
                     /translation="MKVAVLGATGTIGRALVPELARRHEVVAIARRPGPDADGVTWRA
                     ADATDEDALREALEGVEVVHHLVHSLGSPDFEERDRRAADAVARAAAAVGARQIVYLG
                     GLGDDAPDLSPHLRSRAETAERLAAGSVPVTTLRAAMIVGRGSAAFETVRALVERLPG
                     MICPRWVSTETQPIALADVVRYLAGVTGCEAAYGETYDLGGPEVMTYRTMIERIARLR
                     GRRPLIIEVPILSPRLSSLWLHLVTPVRAGVARPLVEGLKNPTVARDTRIRELVPFEP
                     TPFDAAAREAFEPDRS"
     gene            complement(8218..8724)
                     /locus_tag="VFO88_06605"
     CDS             complement(8218..8724)
                     /locus_tag="VFO88_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545231.1"
                     /translation="MQTRAWRLPDGSITTEQRVGLRAPAVPVGEAGALALHRAYWTEV
                     EGLLHGLVRVHEHEAGVDIRLLAGGPALIRLGPRRVTVGRERVTCRYPILGGLLTRRP
                     AGALTLEQVGGPAAELHSSVAGFHPRLAARPGFPPWTGVLYPRIQARLHDAVGRRFLA
                     RLAGGAPS"
     gene            complement(8789..>9030)
                     /locus_tag="VFO88_06610"
     CDS             complement(8789..>9030)
                     /locus_tag="VFO88_06610"
                     /inference="COORDINATES: protein motif:HMM:NF015996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HET9545232.1"
                     /translation="MKVLDIVAAVLLVVGGLNWGLVAVAEFDLVAWIVGLSFGETNAV
                     SRVIYGLVGASAVYQIAQQAAIRRRWSRDPQVAAA"
BASE COUNT         1127 a         3416 c         3388 g         1099 t
ORIGIN      
        1 gtcctcctcg accgcgacgg cacgctcgtc gtggacgtcc cgtacaacgg cgacccgcgg
       61 ctcgtcgagc cggtgccggg cgcgcgagag gccctcgacc ggctccgcga ggccgggatc
      121 ccgctcggca tcgtctcgaa ccagagcggg gtgggacgcg gcctcctcac gctcgaccag
      181 gtcgaggccg tgaaccgccg ggtcgagcag ctcctcgggc cgttcgacgc gtgggccgtg
      241 tgcccgcacg cgccggagga cggctgcgag tgccgcaagc cgcgcggcgg cctcgtccgc
      301 caggcggcgg aggagctcgg cgccgacccg gcccgctgcg tcctcgtcgg ggacatcggc
      361 gccgacgtcg aggccgcgcg cgcggccggc gcccgcgcgg tgctcgtccc gacgtcgctc
      421 acccgccgcg aggagatcga ggccgcgccg gaggtggcgc ccgacctcct gacggccgtg
      481 gagctcgttc tcggaggcgc gcgatgacgc acgtgctcgt ggcgcggcag gacagcgcgg
      541 gcgacgtcct cgtgacgggg cccgcgatcc gcgccgtcgc cgccggcgcc gaccaggtga
      601 cgctcgtctg cggccgccgg ggccgcgcgg cggccgagct ccttccgggc gtggacgcgc
      661 tcgtccagca cacggctccg tggatcgacc cggagccgga gccgctccgc cgggcgccga
      721 cactcgagct cgtcgaggag ctcgccgcgc tcgagctcga cgaggcgatc gtcttcacct
      781 cgttccacca gagcccgctc ccgcttgcgc tgctcctccg gctcgcgggc gtcccgcggg
      841 tctcggcgat ctcggaggac tacccgggct cgctcctcga cgtccgccac cggccgagcg
      901 acgagctgca cgaggtcgag cgagcgctct cgctcgccgc cgcggccggc tacgagcttc
      961 cggacggcga cgacggacgg ctcgcggtcg accggcccgg cacggagccg cccgtcgacg
     1021 agccgtacgt cgtcgtccac ccagggacgt ccgcgccggc ccgcgcgtgg ccgccggagc
     1081 ggcacgccga gctcgtcgcg gccctcgcgg gcgagggacg ccgggtcgtc gtcaccggcg
     1141 cgccggacga gcgggagctg accgagctcg tcgcccgcga cggggggctc gacctcggcg
     1201 gcgcgacgag cctcgccgag ctcgcggacg tcctcgcgca cgccgatgcc gtggttgtcg
     1261 ggaacacggg cccggcgcac ctcgccgccg cggtgggcac gccggtcgtc tcgctcttcg
     1321 cgccgaccgt ccccgccggg cgctggcgcc cgtggggcgt gccgctcgag ctcctcggcg
     1381 accaggacgc gccctgcgcc gggacgcggg cgaccgtgtg cccggttccc ggccacccgt
     1441 gcctctgctc ggtgaccccg cgagacgtgg tcgccgcgct cgagcgcctc cgaggtgtgc
     1501 ggcaggccgc gatcgggcac gcgcggcctt cgatggtgga ggcagcgtcg tgaaggtcgc
     1561 cctcgtctcc gagcacgcga gcccgctcgc cgtgctcggc ggcgtggacg cgggtgggca
     1621 gaacgtccac gtcgccgcgc tcgcgggcga gctggcgagg cgggacgtcg aggtcgtcgt
     1681 ccacacgcgg cgtgacgacc cgggcctgcc cgagcacgtc gagctctgcc ccggcgtcgt
     1741 cgtccaccac gtggacgccg gcccgccgag cgcgatcccg aaggacgacc tcctgccgta
     1801 catggacgac ttcgcccacg tgctccacga cgcgtgggcc gacgcgccgc ccgacctcgt
     1861 ccactcgcac ttctggatgt ccggccgcgc ctcgatccag gccgcgcgcc cgctcgggat
     1921 ccccctcgtc cacaccttcc acgcgctcgg cgtcgtgaag aaacgccatc agggcgcgct
     1981 cgacacgagc ccgcccgggc gcgtcgccgc cgaggagcgg atcgcgcgcc gctgcgacca
     2041 cgtgatcgcg accgccaccg acgaggtctt cgagctcctg cggatgggcg tcgacgtccg
     2101 gcgcgtctcg gtcgtcccgt gtggggtcga cctcgagctc ttccgcccgg tcggcccggt
     2161 cgaggagcgc acgccgcgcc gcgcgcggat cgtcgtcgtg agccggctcg tgcagcggaa
     2221 gggcgtcggg aacgtgatct ccgcgctcgc cgagctgccg ggcgtcgagc tcgtcgtcgc
     2281 gggcggcccg gagcgcgcgg agctcggctc cgacccggag gcgtgccgcc tcgccgcgct
     2341 cgcggacgag ctcgggctcg ccgaccgcgt cgacttccgc ggccgcttgg agcgcgagga
     2401 cctgcccgcg ctcctccgtt ccgccgacgc ggccgtctgc gtgccctggt acgagccgtt
     2461 cgggatcgtc ccgctcgagg cgatggcgtg cggcgtcccg gtcgtcgcgt cggcggtcgg
     2521 cgggctgctg gacaccgtcg tcgacggcga gaccggcgtc ctcgtgccgc cgcgcagccc
     2581 ggagcgtctc gccgaggtgc tccgcgagct gatcgccgac ggcgcggccc gcgagcggat
     2641 gggccgggcc ggcgtcgagc ggacgcgggc gcggtacagc tggggcctcg tcgccgacca
     2701 gacgctgcgg gtgtacgagt cgctgccgcg ccgcccggcg cgccgcccgc tccagctcgt
     2761 cggcaacgga aacgggaacg gcgacggatc gcactccctg cgccggtcga gcgccgagca
     2821 cctcgccgcg tccgtgcgcg ggctcgcgac cctcgtcgcc gagacgcagc ggatcgagag
     2881 ctggggccgg agcctcgccc gcacgctcct ctccggcggc cgcctgctcg cggtcgggaa
     2941 cggcgggagc gcggcgcagg cccagcacct caccggcgag ctcgtcgggc ggtactcgtg
     3001 cgagcgcccc gctctctcgg cgatcccgct gcacggggac acgtccacgc tgaccgcgat
     3061 cgagaacgac tatggccggg aggaggcgtt cgcgcggcag gtgcgcgcgc acgggcggcc
     3121 gggcgacgtg ctcttctgtc tctcgacgtc cggccggagc cccaacgtcc tcgcggccgc
     3181 gcgtgccgcg gccgagggcg gcctgaccgt gtggggcctg accggccccg ccccgaaccc
     3241 gctcgccgac ctgtgcgacg acgtcatccc cgtggacgcg ccgacgccgg caaacgtcca
     3301 ggagctgcac ctcgtcgcgc tccacctgct gtgcgcggcg gtggacgagg aggtcgagac
     3361 ctcggccgcc cacgcggcca cccgacatgt ggaggagctg caccatggca tcgcgtgacc
     3421 tcggaaccgt cgtcgtgacc ggcggcgcat cggggctcgg cgcggcgatc gcccgggcga
     3481 tcgcgcagaa cggcggcacg cccgtcgtcc tcgaccgcgt cgagccgccc tacgacgtcg
     3541 agcacgtcgt cgtcgacctg gccgatcgcg ccgccgcgga agacgccgtg cgcgggtcgg
     3601 ccgagcgcca cggcgggctc gacgccgtcg tcgccgcggc cgggacggac agctgcggca
     3661 agctggacga cgtcccgcgc gacgactggg agcgcgtcgt cctcgtcaac ctgctcggca
     3721 ccgcggcggt cgtccgcgcc gcgctgccct acctggaggc ctccgaggga agggtcgtca
     3781 cggtcgcctc gacgctcggc ctgcgcgcgc tctccgacgc gaccgcctac tgcgcgagca
     3841 agttcggcgt cgtcgggttc acgcgcgcgc tgaccgcgga gacggccggg cgcgtcggcg
     3901 tgacgctcct cctcccgggc gggatgcaaa cgaacttctt cgacgggagg ccggacgagt
     3961 acaagccggg cccggacgcg cagctcaacc ggccggagga cgtggccgac gccgtcctct
     4021 tcgcgctcac ccgccctgcc ggctgcgagg tgcgcgagct gctcgtcgcc ccggcgcggg
     4081 agagctcgtg gccgtagcgg tcgaccggct cgacccgccg ctcccgcagg agctccaggt
     4141 cgaggtcacc ggcgcgtgca acctccgctg ccggatgtgc ctcgtcagct accgcccggc
     4201 gctcgggaag acgagcggcg cgatggacct cgcctcgttc acggagctcc tcgactcgct
     4261 cccggatctc cgcaaggtga cgatgcaggg cctcggcgag ccgcttctcg cgccggactt
     4321 cttcgccatg ctcgagctgc tcgcgtcgcg cgggatcgcg atgggcttca acacgaacgg
     4381 gaccctcctc acgcgcgaga gggccgagcg gctcgtcgac ctgggcgtcg actggctgca
     4441 cgtctcgctc gacggcgcga cggcgccgac gtacgaggac gtccggcgcc gctcgcagtt
     4501 cgagctcgtc cggcgaaacg tcgcgggcct catggaggcg atgcgcgagc gcggcgccga
     4561 gaggcccgag gtcgagctcg tcttcgtcgc catgcggcgc aacgtccacg agctgcccgc
     4621 gctcgtgcgc ctcgcggccg accttcgcgt cccgaccgtc tgggtgcaga acctctcgca
     4681 ctccttcagc gacaccgacc cgtccggcga ctaccgcgag atccgcgagt acgccgaggc
     4741 cgaggcgctc tgggccgagc ggaacgagga ggctgagctt ctcttcgccg aggcggagcg
     4801 ggtcgccgcc gagctcggcg tgcggctgcg gcttccccgg ctcgaggatc ccgcgcgtgc
     4861 gcgggagccc gggcagcccg gctgcgggtg gccgttcgac tccgcgtacg tgacgcacga
     4921 cgggaaggtg cagccgtgct gcgcggtgat gggcgccgac cgcgccgtcc tcggcgacgt
     4981 gaaggcggac ggattcgagc gcgtctggcg gaacgacgcg tatcgcgact tccgcgccgg
     5041 gctcctcggc gacgagccgc ccgccgtctg ccgcggctgc tcgatgtacc gccacgtgtt
     5101 ctaggagggc ggcgctacgg cgtgatgagg cgccgctcgg agatgcagtc ctgcgcccag
     5161 gcctcgacgg cggggacgag cgcgctcgcc tcggccgggt cgtggccggg gagctcgacg
     5221 cgcacctggt agagctcggc gcggcggtcc ggcccgaagc tctcgcgccg ctcgcagacg
     5281 acctcgagcg acgacacgcg ctcgagcagc tccgtcccga ggcgctcgac ctccgcgatc
     5341 gtcgcctcgc ggccggcgta cacgccgaag ttgacgacga ttccgggccg cccgtcgatc
     5401 tcggcggcga atgcgagctc cgcttcctgc atcggggtga ctatgcctcg cggggctccc
     5461 atctgaacat ccgcaccgcg aggacgagcc ctgcaagccc ccagccggcg aggacggcga
     5521 tcgcctcgag cgcccctccg agcccctcgc cgccggcgac gacgcactcc cgcacgaggc
     5581 gcaggaggta cgtgaggggc gagatctccg cgaccgcctg gaggaacccg ggcaacgagt
     5641 cgaccgagaa gaacacgccg gagatgaaga cgagcgggag gtagatcgcg ttcacgagcg
     5701 cggacgagcc ctcggcgctc cggatcaggc ccgtgatcgc caggccgagc gcggcgaagc
     5761 acgcggcgcc gacgagcagc gcgaccgcga gctcgccggg ccgcgacggc cagccggcat
     5821 cgacgacgaa ccgcccgacg aggagctgcg cgatgcttcc gagcgcgatc acgacgagcg
     5881 tcgagccgat caccgccgcg aggaaggtcg ccgtcggcag cggcgtgccg cgcacgcgct
     5941 tcagcacgcc cgactcgcgc cgcacgacga gcgtgatggc gagcccggcg aacgcggtcg
     6001 cgatcaccga gtagccgagc agcccggcga ggaggtacgt cgagccgcgg attccgtcca
     6061 cctcgcccga cccgtagacc gagccgatca ggacgaggaa gacgagcggc atgacgaacg
     6121 tgaagaacgc cgcctcgcgg ctgcgccagt agagccgctg ctgcgcggcg agctcgtgga
     6181 ggaagagcct cactcgccgt cccgcgtgag ctcgaggtag agatcctcga gcgtgcgccg
     6241 gcgcacctgg atgctgtcga gctcccggcc ggcggcgagc gcgtcggccg tcagctcgtt
     6301 gaggacgcgc gtcggctcct cggtctcgag cacgacctcc tccgcgccgg agcggtagcg
     6361 gatctcgaca gtcgcgtggc cgtcgagcag gtcgcgcggc gagccggtcg cgacgatccg
     6421 cccgtcgcgg aggatcgcca gccggtcggc gagcgtctgc gcctcctcca tgtagtgcgt
     6481 cgtcaggagg atcgtcttgc cgagcgcgcg gaggccgcgg atcgtctccc aggcgcggcg
     6541 gcgcgcggcc gggtcgaacc cggtggtcgg ctcgtcgaga aagacgagct ccgggtcgcc
     6601 gacgagcgcg aggccgaggt cgagccggcg gagctggccg ccgctcagcg tccgcacgcg
     6661 cgcgtcgcgc ttctcctcga ggccgacgag gcgcacgacc tcgcccggct cgcgcggcga
     6721 cgggtagtag ccggcgaaga gctcgaggag ctcgccgacc gagagcgtgg ggtacacggc
     6781 ggagctctgg aggacgatgc cgatccgggc ccggaagtcg cccgtcgccc ggccggggtc
     6841 gacgccgagg acgcgcgcct cgccgccgtc gcgctcgcgg tagccctcga ggatctcgac
     6901 cgtcgtcgtc ttgcccgcgc cgttcgggcc gaggagcgcg aagacctccc ccgcgggcac
     6961 gtcgaggtcg agcccgtgca ggacctcgcg cgggccgtac gacttgcgca ggccgcggat
     7021 ctcgatcgcg tgctccaccg cgggcgagga tatccgggtc ccctcggcgg ccggtcgggt
     7081 gcgctgtgga gggatcggca cgcccacgtc gcactccggc acactctcgc gcgcttctcg
     7141 acgatacgct cgtcgcggcg aggggcggta cccccacccc gggtgggcgc gcgcgcgtcg
     7201 agcccacccg accgcacgag cgttggcacg cgcgggacga cgtgctgctc ggcgcgtccg
     7261 ggaaggacgt tgccggcggg atgggcggaa acggccggtc cgacggtcgc gaccgtgtga
     7321 cgtcggccca gcggcggcgg ttctaggagc gatccggctc gaacgcctcg cgggcggcgg
     7381 cgtcgaacgg cgtcggctcg aacggaacga gctcgcggat gcgggtgtcc cgcgcgaccg
     7441 tcgggttctt cagcccctcg acgagcggcc gggcgacccc ggcccgcacc ggcgtcacga
     7501 gatgaagcca gagcgacgag agccgcgggc tgaggatggg cacctcgatg atcaacggcc
     7561 gccggccgcg gagccgcgcg atccgctcga tcatcgtccg gtacgtcatc acctcgggcc
     7621 cgccgaggtc gtacgtctcg ccgtaggccg cctcgcagcc ggtcacgccg gcgaggtagc
     7681 ggacgacgtc ggcgagcgcg atcggctgcg tctcggtcga gacccagcgc gggcagatca
     7741 tcccgggcag ccgctcgacg agcgcgcgca ccgtctcgaa cgcggcgctc ccacgcccca
     7801 cgatcatcgc ggcgcgcagg gtcgtgaccg gcaccgagcc ggccgcgagc cgctcggccg
     7861 tctcggcccg gctgcgaaga tgcggcgaga ggtcgggcgc gtcgtcgccg agcccgccga
     7921 ggtacacgat ctgcctcgct ccgacggcgg cggcggcgcg ggcgaccgcg tcggcggcgc
     7981 gacggtcgcg ctcctcgaag tccggcgagc cgagcgagtg cacgaggtgg tggacgacct
     8041 cgacgccctc gagcgcctcg cggagcgcgt cctcgtcggt cgcgtcggcc gcgcgccagg
     8101 tcacgccgtc ggcgtccggc ccggggcgcc gcgcgatcgc gacgacctcg tgccggcgtg
     8161 cgagctcggg gacgagcgcg cggccgatcg tcccggtcgc gccgagcacg gcgaccttca
     8221 cgacggcgct ccgccggcca gccgcgcgag gaagcgccgc ccgaccgcgt cgtggagccg
     8281 cgcctgtatg cggggataga ggacgcccgt ccacggagga aagccgggcc gcgcggcaag
     8341 gcgtggatgg aagccggcca ccgacgagtg cagctccgcg gccggcccgc cgacctgctc
     8401 gagcgtgagc gctcccgccg gccggcgcgt caggagcccg ccgaggatcg ggtaccggca
     8461 cgtcacccgc tcccgcccga ccgtcacgcg gcgcgggccg agccggatga gcgcgggccc
     8521 gcccgcgagc agccggatgt cgacgccggc ctcgtgctcg tgcacgcgca cgagcccgtg
     8581 gagcaaccct tccacctcgg tccagtatgc gcggtgcagc gcgagcgcgc cggcctcgcc
     8641 gaccggtact gccggcgccc ggaggccgac gcgctgctcg gtcgtgatcg acccgtcagg
     8701 gaggcgccag gcgcgggtct gcaccgcgcc gctccgggag ggccggccgg cacgtgccgc
     8761 ccggccctcc ggctccggct gtggtgcgtt aggcggcagc gacttgcggg tcgcggctcc
     8821 agcggcggcg aatcgcagcc tgctgggcga tctggtagac ggcgcttgcg ccgacgaggc
     8881 cgtagatgac gcggctgacc gcgttcgtct cgccgaagct gagcccgacg atccaggcca
     8941 cgaggtcgaa ctccgcgacc gcgacgagcc cccagttgag gccgccgacg acgagcagga
     9001 cggcggcgac gatgtcgaga accttcatgt
//
LOCUS       DASTFC010000221         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_531948,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000221 DASTFC010000000
VERSION     DASTFC010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531948"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFO88_06615"
     CDS             complement(<1..622)
                     /locus_tag="VFO88_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9545233.1"
                     /translation="MRVAIAGGHGGIGLRLTRLLSGRGDEVLALIRNPEHSGDVREAG
                     GEPVLCDLEAADVREVEAAIGSADAVVFAAGAGPGSGSARKETMDYGGAVKLIAAATA
                     AGVARYVMVSSMGADPDVQGDDTFSVYLRAKGRADAELAASGLDYTILRPGRLTDEPG
                     TGRVRLGEGVPRGAVPRDDVAAALAEILHAPATVGTTVGLTSGDTPV"
     gene            complement(633..1817)
                     /locus_tag="VFO88_06620"
     CDS             complement(633..1817)
                     /locus_tag="VFO88_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545234.1"
                     /translation="MNEPQELSRDYIRLGLALDRHVDGFADAYFGPEELREEAQQGPP
                     REPARLADDADRLLERVAADGLDPQRARWLTAQLSACRTVAGRLAGEAPSWEEEVERC
                     FGIRPARVSEERFREAHALLDDALPPGGSLRDRYAAWIDAQLVPPERLVEAAEHVAAL
                     LRDATRELVDLPVEEGFDLELAEDVPWLAFNFYLGGFRSRVALNRGRPIWAPWLVDVV
                     AHELYPGHHTERVVKERRLVRERGFLEETIVLALAPQALVMEGIAMLALETALGREAH
                     EPAAERLRPLGLGYDVEGAAPVQRAEALLEEVGLNAARMLHQDGRPVEEVREYVATWS
                     VKPAAVVDHIVEFVRDPTWRTYIATYSCGLTLCRRYVDGSRERFATLLSEQLTPAELQ
                     AD"
     gene            complement(1891..2235)
                     /locus_tag="VFO88_06625"
     CDS             complement(1891..2235)
                     /locus_tag="VFO88_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545235.1"
                     /translation="MRGVLVAALAALLALAGASVARADHTHVLILPNGNCAILAAQGN
                     EKYVVLPDVLFANNPNADADDAGGLENLPMNRRHPLHVLVHLGVPGADGDIAVMGSAQ
                     DPCAATGNYVNG"
     gene            complement(2311..2871)
                     /locus_tag="VFO88_06630"
     CDS             complement(2311..2871)
                     /locus_tag="VFO88_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9545236.1"
                     /translation="MGRLVYTAIASLDGYVRDADGSFAWAEPTEEVHAFVNERERPVG
                     TYLYGRGMYETMAVWETEPALAEASPVMAEYAELWRAADKVVYSTTLDSVATGRTRLE
                     RTFDAGAVARLKKEAPSDLAVAGPTLAAEAFRAGLVDELQLYVVPVVVGGGLPALPDG
                     VRLDLELLEERRFAGGFVFLRYAARR"
BASE COUNT          393 a         1127 c         1122 g          381 t
ORIGIN      
        1 cgaccggagt gtcgcccgac gtgagcccga cggtggtacc cacggtcgct ggcgcgtgca
       61 ggatctcggc gagcgccgcg gccacatcgt cgcgggggac ggcgccgcgc ggcacgccct
      121 cgccgagccg cacccggccc gtgccgggct cgtcggtcag cctgccgggg cggaggatcg
      181 tgtagtcgag cccgctcgcc gcgagctcgg cgtcggcgcg gcctttcgca cgcaggtaga
      241 cactgaaggt gtcgtcgccc tgcacgtcgg ggtcggcgcc catggagctg accatcacgt
      301 agcgcgcgac gccggccgcg gtcgcggcgg cgatgagctt cacggcgccg ccgtagtcca
      361 tcgtctcctt gcgggcggag ccgctgccgg gaccggcgcc ggcggcgaag acgaccgcgt
      421 cggccgaccc gatcgccgcc tcgacctcgc gtacgtcggc cgcctcgaga tcgcagagga
      481 cgggctcccc gccggcctcg cggacgtcgc ccgagtgctc ggggttgcgg atcagcgcga
      541 ggacctcgtc gccgcggccg ctcagcagcc gcgtgagccg aagcccgatc ccgccgtgtc
      601 ctcccgcgat cgcgacccgc acccgggcga tgctagtcgg cctggagctc ggccggcgtc
      661 agctgctcgg agaggagcgt cgcgaaccgc tcgcgcgagc cgtccacgta gcgccggcac
      721 agcgtgagcc cgcagctgta cgtggcgatg tacgtgcgcc aggtcgggtc gcggacgaac
      781 tcgacgatgt gatcgacgac cgccgcgggc ttcaccgacc aggtcgcgac gtactcgcgg
      841 acctcctcca ccggccggcc gtcctggtgg agcatccggg cggcgttcag gcctacctcc
      901 tcgagcagcg cctccgcccg ctggacgggc gcggcgccct cgacgtcgta cccgaggccg
      961 agcggccgca gccgctccgc ggccggctcg tgcgcctccc gtccgagcgc cgtctcgagc
     1021 gcgagcatcg cgatcccctc catgacgagc gcctgcggcg cgagggcgag cacgatcgtc
     1081 tcctcgagga agccgcgctc gcggacgagg cgccgctcct tcacgacccg ctcggtgtgg
     1141 tggcccggat agagctcgtg cgcgacgacg tcgacgagcc acggcgccca gatcggccgg
     1201 ccccggttga gcgcgacccg gctgcggaag ccgccgaggt agaagttgaa ggcgagccag
     1261 ggaacgtcct cggcgagctc gaggtcgaag ccctcctcca ccggcaggtc gacgagctcg
     1321 cgcgtcgcgt cgcgcagcag cgcggcgacg tgctccgccg cctcgaccag ccgctcgggc
     1381 gggacgagct gggcgtcgat ccacgcggcg taccggtcgc ggagcgagcc gcccggcggg
     1441 agcgcgtcgt cgaggagcgc gtgcgcctcg cggaaccgct cctcggacac ccgcgccgga
     1501 cggatcccga agcagcgctc gacctcctcc tcccaggacg gcgcctcgcc cgcaaggcgg
     1561 cccgcgaccg tccggcacgc cgagagctgc gccgtgagcc agcgtgcgcg ctgcggatcg
     1621 agcccgtcgg cggcgacgcg ctcgaggagc cggtcggcgt cgtcggcgag gcgggccggc
     1681 tcgcggggcg ggccctgctg cgcctcctcg cggagctcct ccggtccgaa gtacgcgtcg
     1741 gcgaagccgt cgacgtgccg gtcgagcgcg agcccgagcc ggatgtagtc gcgcgagagc
     1801 tcctgcggct cgttcacggc cgcgagtcta ggccgtggag ccgcgggcga gggcgccgtg
     1861 ccgcgcgggc gcggcgccct cgccgggcgg tcagccgttg acgtagttcc cggtcgcggc
     1921 gcacgggtcc tgggcggagc ccatgaccgc gatgtcgccg tcggcgcccg ggacgccgag
     1981 gtgcacgagc acgtggagcg gatggcgccg gttcatcggc aggttctcga gcccaccggc
     2041 atcgtccgcg tccgcgttcg ggttgttcgc gaagagcacg tccgggagga cgacgtactt
     2101 ctcgttgccc tgggccgcga ggatcgcgca gttcccgttc ggcaggatca gcacgtgcgt
     2161 gtgatccgcc cgggcgacgc tcgcccccgc gagcgcgagg agggccgcaa gcgcggcgac
     2221 aagaactccc ctcattcctc tcctccttcc tggtaaggac ctgacgcgcg tcatgatttc
     2281 cgaggtcgcg cgggttgtcc aacggaaggg ctatcgccgc gccgcgtagc gcagaaacac
     2341 aaagccgccg gcgaagcggc gctcttcgag gagctcgagg tcgagccgga cgccgtccgg
     2401 gagcgcgggc agcccgccgc cgacgacgac cgggacgacg tagagctgga gctcgtccac
     2461 gagcccggcg cggaaggcct cggctgcgag ggtcgggccc gcgaccgcga ggtccgacgg
     2521 cgcctccttt ttgagccgcg cgacggcgcc cgcgtcgaac gtccgctcga gacgcgtccg
     2581 cccggtcgcg accgagtcga gcgtcgtcga gtacacgacc ttgtcggccg cgcgccagag
     2641 ctccgcgtac tcggccatga cgggcgaggc ctccgcgagc gcgggctcgg tctcccagac
     2701 ggccatcgtc tcgtacatgc cgcggccgta caggtaggtg ccgacgggtc gctcccgctc
     2761 gttcacgaac gcgtgcacct cctcggtcgg ctcggcccag gcgaaggagc cgtcggcgtc
     2821 gcggacgtag ccgtcgagcg acgcgatcgc cgtgtagacg agcctgccca ctaacaccag
     2881 tccgcgcgcg tgagcggcag ggccgcgtcg ccgtccgccg gcttggcgag gagcgcctgg
     2941 aagacgctca gccggcccgc cgcgaacgag tgcgccgagc cggccatgta gagccgccag
     3001 acgcggtacg cgagctcgcc ctc
//
LOCUS       DASTFC010000222         5041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_433270,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000222 DASTFC010000000
VERSION     DASTFC010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433270"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="VFO88_06635"
     CDS             complement(<1..2004)
                     /locus_tag="VFO88_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9545237.1"
                     /translation="MQRNRLARLVAPLVALAAFAVAPPALAQSEAPVPERYEDLLETA
                     REDGSVRVLVATNGMLDRSIVGSAADAAGGEILTRYRNFPILLVEATPAALVELAQSP
                     FVLGLEEDVPEPAALASTLPLINGDDVHGFGYDGDGTAVAILDTGIDADHPYFAGRIV
                     SQACFSNAGGAGGGTSICAGGGTAETNAGSANIEIAACMDGTVNICDHGSHVAGIAAG
                     EATGAAGAPGDGVAPGADLIAINVFTRFDSAADCSPNPAPCVSSFPSDQVLGLDRVLA
                     LDGTLASDIVAANMSLGGGMFTANCDGDNQGRKAAIDALLAEEIATIVAAGNNGFNAA
                     VSRPGCVSTAVTVGATTDGDAVAGFSNRGTLLDLFAPGVGVDSSVPDDTWANFDGTSM
                     ATPHVTGAWGVLRDMYPSASVATILGWLQANGQAITYMSGGSNVTTPRIDLLATVQPD
                     LAANAASVTVDEGQTATNSGTFADPNGDAVTISASVGTVVQGAGTWSWSFDTVDGPAD
                     SQVVTITATDALGIESELTFPLTVLNVEPSVSNDPAQVTAIDEGDVLDVLSLFSDPGY
                     LDNPYSAVVDWGHADLGTSPGAIVMTQDAGPGPDEGEVSASKAYGDNGSFTVTTTVTD
                     KDGGSGDTSFGLTVANVDPTAAIDLSGTVLVNGVPTLIGDLDGP"
     gene            complement(2119..2325)
                     /locus_tag="VFO88_06640"
     CDS             complement(2119..2325)
                     /locus_tag="VFO88_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545238.1"
                     /translation="MTERQPSPAEAARRQRCRSLELAAVLGIVLGVVSVVDRSWPGVA
                     VGVAGAALAWGVWWRRCRDAPDAS"
     gene            complement(2322..3575)
                     /locus_tag="VFO88_06645"
     CDS             complement(2322..3575)
                     /locus_tag="VFO88_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545239.1"
                     /translation="MHAPRSPSLLRALRAFCLGAFFELGRELESGADLPISLAEHTSP
                     NRPTLYEYRPLVGAFVEARAERLGRREDALEALAALKDEPAAGIFARAHAGEKTSEDE
                     ALRRTVLVPLLVRTAEACGGFDWEDSAFERAYAELERSLFGATRAYGAVVPLVGLSAG
                     GTIELGPGLRVRHAATNELQAGWPEAQRLLPRDFGREVDRTLVLELETELEGTACVVP
                     DAPVVVGRAITVLRLVTAGAIAAGPVVFERLDWRPYDVRPAPPLAAQVPPGEPTRIDP
                     FRARLVAQLVERLAAGVDADVHEALDRWELALFHAGPVRADELREALESLLGFDEGPW
                     AAAMRAAVLLGENGRERDELRLALRALLDGDGPGARAEEAVRRSLVETLLHDSRPGLI
                     AALDEVLLGVRPRAGTITGRVVAAG"
     gene            complement(3625..4401)
                     /locus_tag="VFO88_06650"
     CDS             complement(3625..4401)
                     /locus_tag="VFO88_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9545240.1"
                     /translation="MPEGDSLHRAAARLTPLVGEPIAVEARHGRARALGIAEQLDGRR
                     LESVRAVGKNLLLEWEGGLVLRSHLRMKGRWRVAAAGAPIVGTPWLVLTGTRLQAVLS
                     GGGELELTRGPNPRVARLGPDVMLDPPDLEGMLARFRGTDQERELGEALLDQRLAAGI
                     GNMWRAEGLFRARLSPWARLRDVSDDDVRRVLAETSAAMRSRRARRSVYDRAGLPCPR
                     CATPIAAWRQGDDARTAFWCPACQPAPAGSRRVGDEAPAR"
     gene            4485..>5041
                     /locus_tag="VFO88_06655"
     CDS             4485..>5041
                     /locus_tag="VFO88_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HET9545241.1"
                     /translation="MAAAVRWSARAGLVGGALAASVLLAWAPGASAALKPRATVADLS
                     VLEGRKAVFVVKLNRAPRTAVRVRFKTRNGAALAGVDYKARSGVLRFKARQRSKKVAV
                     VTLNDGTWEPNEAFFLRLSAPLGVVLKRATARTRIVDDDPLPSLSVADVTVVEGNGGQ
                     APASFAVTLTGATSRTVTVAYETSDG"
BASE COUNT          629 a         1844 c         1942 g          626 t
ORIGIN      
        1 cggcccgtcg aggtccccga tgagcgtcgg gaccccgttc acgagcaccg tcccgctcag
       61 gtcgatcgcc gcggtcgggt cgacgttcgc gacggtgagc ccgaagctcg tgtcgccgga
      121 gccgccgtcc ttgtccgtga ccgtggtggt gaccgtgaac gagccgttgt cgccgtacgc
      181 cttcgacgcg gagacctcgc cctcgtcggg gccggggccc gcgtcctgcg tcatcacgat
      241 cgcgccgggg ctcgtgccga ggtcggcgtg gccccagtcg acgaccgcgg agtacgggtt
      301 gtcgaggtag cccgggtccg agaagagcga gaggacgtcg aggacgtcgc cctcgtcgat
      361 cgccgtcacc tgcgccgggt cgttcgagac gctcggctcg acgttcagga cggtgagcgg
      421 gaacgtgagc tcgctctcga tcccgagcgc gtccgtcgcg gtgatcgtca cgacctggct
      481 gtcggccggg ccgtcgacgg tgtcgaagct ccacgaccag gtgccggcgc cctgcacgac
      541 cgtcccgacg gaggcggaga tcgtcaccgc gtcgccgttc gggtcggcga acgtgccgct
      601 gttcgtcgcc gtctggccct cgtccaccgt gacgctcgct gcgttcgcgg cgaggtccgg
      661 ctggacggtg gcgagcaggt cgatccgcgg cgtcgtcacg ttcgagccgc cgctcatgta
      721 cgtgatcgcc tgcccgttcg cctggagcca gccgaggatc gtggcgacgc tcgcgctcgg
      781 gtacatgtcc cgtaggactc cccaggcgcc cgtaacgtgc ggggtcgcca tcgacgtgcc
      841 gtcgaagttc gcccacgtgt cgtccggcac cgacgagtcg acgccgacgc ccggcgcgaa
      901 caggtcgagg agcgtgccgc ggttcgagaa gccggcgacc gcgtcgccgt cggtcgtcgc
      961 gccgacggtc acggccgtcg agacgcagcc cggccgggag acggcggcgt tgaagccgtt
     1021 gttcccggcc gcgacgatcg tcgcgatctc ctcggcgagg agcgcgtcga tcgcggcctt
     1081 gcgtccctgg ttgtctccgt cgcagttcgc cgtgaacatg ccgccgccga ggctcatgtt
     1141 ggcggcgacg atgtccgacg cgagcgtccc gtcgagcgcg agcacccggt cgaggccgag
     1201 cacctggtcg gacgggaacg agctcacgca cggcgccggg ttcgggctgc agtccgcggc
     1261 gctgtcgaag cgggtgaaga cgttgatcgc gatgaggtcc gcacccggcg cgaccccgtc
     1321 gccgggggcg ccggccgcac ccgtcgcctc gccggccgcg atcccggcca cgtgcgagcc
     1381 gtggtcgcag atgttcacgg tgccgtccat gcacgccgcg atctcgatgt tcgccgagcc
     1441 ggcgttcgtc tccgcggtgc cgccgccggc gcagatgctc gtcccgccgc cggcgccgcc
     1501 ggcgttcgag aagcaggcct gcgacacgat ccggcccgcg aagtacgggt ggtcggcgtc
     1561 gatgcccgtg tcgaggatcg cgaccgcggt gccgtcgccg tcgtagccga acccgtgcac
     1621 gtcgtcgccg ttgatgagcg gcagcgtgct cgcgagcgcg gcgggctcgg gcacgtcctc
     1681 ctcgaggccg aggacgaacg ggctctgcgc gagctcgacg agcgccgcgg gcgtcgcctc
     1741 gacgaggagg atcgggaagt tgcggtagcg cgtcaggatc tccccgcccg cggcgtcggc
     1801 ggcgctgccg acgatgctgc ggtcgagcat cccgttcgtg gcgacgagga cccggaccga
     1861 gccgtcctcg cgggccgtct cgaggaggtc ctcgtaccgc tcgggcaccg gcgcctcgct
     1921 ctgggcgagg gcgggcggtg cgaccgcgaa ggcggcgagc gcgacgagtg gcgcgaccag
     1981 gcgcgccagt cggttgcgtt gcatcgtgac tcccttctgg atctgggggg aatgcgactg
     2041 ctgcgccgcg ttctacgcag gcccggcccg aatcgcccta accccccggg ccagaatctt
     2101 ccagcggggc gggtggtcct acgaggcgtc cggcgcgtcg cggcagcggc gccaccagac
     2161 gccccaggcg agcgcggcgc cggcgacgcc gaccgcgacc ccgggccagc tccggtcgac
     2221 gaccgagacg acgccgagca cgatcccgag cacggcggcg agctcgagcg accggcagcg
     2281 ctggcggcgc gccgcctccg ccggggacgg ctgccgctcg gtcatccggc ggcgacgacg
     2341 cggccggtga tcgtcccggc gcgcgggcga acgccgagca gcacctcgtc gagcgccgcg
     2401 atcaggccgg ggcgggagtc gtgcagcagc gtctcgacga gcgagcggcg aacggcctcc
     2461 tcggcgcggg caccgggacc gtcgccgtcg aggagcgcac gcagggcgag gcggagctcg
     2521 tcgcgctcgc gcccgttctc gccgaggagg acggcggcgc gcatcgcggc ggcccacggg
     2581 ccctcgtcga agccgagcag cgactcgagc gcctcgcgca gctcgtccgc ccggaccggg
     2641 ccggcgtgga agagcgcgag ctcccagcgg tcgagcgcct cgtggacgtc ggcgtcgacg
     2701 cccgcggcga ggcgctccac gagctgcgcg acgagccggg cgcggaaggg gtcgatgcgg
     2761 gtcggctcgc ccggcggcac ctgcgcggcg agcggcggcg cgggccggac gtcgtacggc
     2821 cgccagtcga gccgctcgaa gacgacgggg ccggcggcga tcgccccggc ggtgacgagg
     2881 cgcaggacgg tgatcgcgcg cccgacgacg acgggcgcgt cggggacgac gcaggcggtt
     2941 ccctcgagct ccgtctcgag ctcgagcacg agcgtgcggt cgacctcgcg cccgaagtcg
     3001 cgcgggagca ggcgctgcgc ctccggccag cccgcctgca gctcgttcgt cgcggcgtgg
     3061 cgcacgcgca ggcccgggcc gagctcgatc gtcccgccgg cggagaggcc gacgagcggc
     3121 acgaccgcgc cgtacgcgcg cgtcgcgccg aagagcgagc gctcgagctc cgcgtacgcg
     3181 cgctcgaagg cggagtcctc ccagtcgaag ccgccgcagg cctcggccgt gcgcacgagg
     3241 agcgggacga ggacggtgcg gcgaagcgcc tcgtcctcgc tcgtcttctc gcccgcatgg
     3301 gcgcgcgcga agatccccgc ggccggctcg tccttgagcg ccgcgagcgc ctcgagcgcg
     3361 tcctcgcgcc ggccgagccg ctcggcccgc gcctcgacga acgcgccgac gaggggccgg
     3421 tactcgtaga gcgtcggccg gttcggcgag gtgtgctcgg cgagcgagat cggcaggtcg
     3481 gcacctgact cgagctcgcg gccgagctcg aagaatgcgc cgaggcagaa ggcgcggagg
     3541 gcgcggagga gcgacggtga gcggggcgcg tgcacggcca cccgactcgg tacggggcgg
     3601 cggcgcgcgt tcctgccttt cgcgtcagcg ggcgggcgcc tcgtcgccga cccgccggct
     3661 cccggcgggc gcgggctggc aggcggggca ccagaacgcc gtgcgcgcgt cgtccccctg
     3721 ccgccaggcg gcgatcggcg tcgcgcagcg cgggcacggg agcccggcgc ggtcgtacac
     3781 ggagcggcgc gcgcgccggg agcgcatggc ggccgaggtc tccgcgagga ctcggcggac
     3841 gtcgtcgtcg gagacgtccc gcagccgcgc ccacggcgag aggcgggcgc ggaagagccc
     3901 ctccgccctc cacatgttcc cgatccccgc cgcgagccgc tggtcgagaa gggcctcgcc
     3961 gagctcgcgc tcctgatccg tgccccggaa gcgggcgagc attccctcga ggtcgggcgg
     4021 atcgagcatg acgtccgggc cgaggcgggc gacgcgcgga ttcggcccgc gcgtcagctc
     4081 gagctcgccg ccgccggaga gaaccgcctg gagccgcgtg cccgtgagga cgagccacgg
     4141 cgtcccgacg atcggcgcgc cggccgccgc gacccgccag cgacccttca tccgcaggtg
     4201 gctccggaga acgagcccgc cctcccactc gagcaggagg ttcttcccga ccgcgcggac
     4261 ggactcgagc cggcgtccgt cgagctgctc cgcgatcccg agggcccgcg cgcggccgtg
     4321 ccgcgcctcg accgcgatcg gctcgccgac gaggggcgtg aggcgcgcgg cggcgcggtg
     4381 gagcgagtct ccctcgggca cgcgaccgag ggtagcgccc tccccgtttt ggaggactcc
     4441 cggcttgagc tttccgcggc ggacgccgac aacggggaag cgcaatggct gcggcagtga
     4501 gatggagcgc ccgagccggg ctcgtcggcg gtgccctcgc ggcgtcggtc ctcctcgcct
     4561 gggcgcccgg cgcctccgcg gccctgaagc cgcgcgcgac cgtcgccgac ctgagcgtcc
     4621 tcgagggcag gaaggcggtg ttcgtcgtga agctgaaccg ggccccgagg acggcggtcc
     4681 gggtgcgctt caagacgcgc aacggcgcgg cgctcgcggg cgtcgactac aaggcgcgct
     4741 ccggcgtcct gcgcttcaag gcgcgccagc ggtcgaagaa ggtcgcggtc gtcacgctga
     4801 acgacggcac gtgggagccg aacgaggcgt tcttcctgcg cctctccgca ccgctgggcg
     4861 tcgtgctgaa gcgggcgacc gcccgcacgc ggatcgtgga cgacgatccg ctcccctccc
     4921 tctccgtcgc cgacgtgacc gtcgtggagg gaaacggcgg ccaggcgccc gcgtcgttcg
     4981 ccgtgacgct cacgggcgcg acgagcagga ccgtgaccgt cgcctacgag acctccgacg
     5041 g
//
LOCUS       DASTFC010000223         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_763075,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000223 DASTFC010000000
VERSION     DASTFC010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763075"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFO88_06660"
     CDS             <1..307
                     /locus_tag="VFO88_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545242.1"
                     /translation="PDRPEGGRRMTALAQTSWPDASIAIVGILFVAALSVVAIWQIFA
                     TAKTRMAVSREQAYKRLAEEAVQVQSKTAEELARASAELTELRRQTGELERMLKEVP"
     gene            584..>2017
                     /locus_tag="VFO88_06665"
     CDS             584..>2017
                     /locus_tag="VFO88_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9545243.1"
                     /translation="MAVATSTSAETRLRELLARRIAVLDGAWGTMLQGRGLTEEDFRG
                     ERFRDHPSDLKGDPDLLNLTRPELVLDVHRAYLEAGADITTTNTFTATAIAQADYGLE
                     DAVYDMNVAGARLARQAADEAGGRFVAGSVGPLNVALSLSPRVDDPAYRAKTFDEVHD
                     AYAEQIRGLRDGGADFLLVETIFDTLNAKAAIAAACETAPELPLWLSVTIVDLSGRTL
                     SGQTLEAFWVSVEHAEPLVVGVNCSLGAKEMRPHVEELARLAHTFTSCHPNAGLPNAF
                     GGYDEGPDTTSALLRGFAEEGLVNLVGGCCGTTPEHTRAIAAAVAGIPPRRVPARRRT
                     TRYAGLEPFELYEDTTFTMIGERTNVTGSARFRELVEGGDFEGALEVALEQVRGGANI
                     LDVNMDADLLESEAAMTRFLNLVATEPEVARLPIMIDSSRWSVVQAGLRCVQGKAIVN
                     SVSLKEGEEDFLAKAREARRFGAALVVM"
BASE COUNT          300 a          713 c          723 g          281 t
ORIGIN      
        1 ccctgaccga cccgaaggag gaaggcgaat gacggcactg gcgcagacga gctggcccga
       61 cgcgagcatc gcgatcgtcg ggattctgtt tgtcgccgcg ctctcggtgg tggcgatctg
      121 gcagatcttc gccacggcca agacgcgcat ggccgtctcc cgcgagcagg catacaagcg
      181 gctcgccgag gaggcggtgc aggttcagtc caagaccgcc gaggagctcg cgcgggcgag
      241 cgcggagctc accgagctgc gccggcagac cggagagctc gagcggatgc tgaaagaggt
      301 gccctaggcc cgttcaggcc ccgttgcgca cgattacgac ggcgttaagc gccggcgcga
      361 ggccttgatc gtggccgccg gcccggtagg ttggagcgcg acccacccgc tccgagcggc
      421 cattggcgtg aatgacgcct ccctctcctg ggttgctcgg gctcttcaac gcgagagcgc
      481 ccccgtcccc ccgggggcgt ttctccgtta aggggcctct ttttcagagc caagcctcga
      541 acgtccttcc gagcggggct ccaggaggca cacttcagag gagatggccg tcgccacctc
      601 cacctctgcg gaaacacgcc tgcgggagct gctcgcgcgc cgcatcgcgg tgctcgacgg
      661 cgcgtggggg acgatgctgc agggtcgcgg cctcacggag gaggacttcc gcggcgagcg
      721 cttccgcgac cacccgagcg acctgaaggg cgaccccgac ctcctgaacc tcacgcggcc
      781 cgagctcgtc ctcgacgtcc accgcgccta cctcgaggcc ggcgccgaca tcacgacgac
      841 gaacacgttc accgcgaccg cgatcgcgca ggccgactac gggctcgagg acgcggtcta
      901 cgacatgaac gtcgcgggcg cgcggctcgc gcggcaggcg gccgacgagg ccggcgggcg
      961 cttcgtcgcc gggtcggtcg gcccgctgaa cgtcgcgctc tcgctctcgc cgcgcgtgga
     1021 cgaccccgcc taccgggcga agaccttcga cgaggtccac gacgcatacg cggagcagat
     1081 tcgcggtctg cgggacggcg gcgccgattt cctgctcgtc gagacgatct tcgacacgct
     1141 gaacgcgaag gcggcgatcg cggccgcgtg cgagaccgcg ccggagctac cgctctggct
     1201 ctccgtgacg atcgtcgacc tgagcgggcg cacgctctcg ggccagacgc tcgaggcgtt
     1261 ctgggtctcc gtcgagcacg ccgagccgct cgtcgtcggg gtcaactgct cgctcggcgc
     1321 gaaggagatg cgcccgcacg tcgaggagct cgcgcgcctc gcgcacacgt tcacgagctg
     1381 ccatccgaac gccggactgc cgaacgcctt cggcggctac gacgagggcc cggacacgac
     1441 ctcggcgctc ctccgcggct tcgccgagga ggggctcgtc aatctcgtcg gcggctgctg
     1501 cggcacgacg cccgagcaca cgcgcgcgat cgccgccgcc gtggccggga tcccgccgcg
     1561 ccgcgtgccc gcgcgccggc gcacgacgcg ctacgcgggc ctcgagccgt tcgagctcta
     1621 cgaggacacg acgttcacga tgatcggcga gcggacgaac gtgaccggct ccgcgcggtt
     1681 tcgcgagctc gtcgagggcg gcgacttcga gggcgcgctc gaggtcgcgc tcgagcaggt
     1741 gcggggcggc gcgaacatcc tcgacgtgaa catggacgcc gacctcctgg agtcggaggc
     1801 ggcgatgacg cgcttcctca acctcgtcgc gaccgagccg gaggtggcgc gcctcccgat
     1861 catgatcgac agctctcgct ggtcggtcgt ccaggcgggc ctgcgctgcg tgcaggggaa
     1921 ggcgatcgtg aactcggtca gcctgaagga gggggaggag gacttcctag cgaaggcgcg
     1981 cgaggcgcgg cgcttcggcg cggcgctcgt cgtcatg
//
LOCUS       DASTFC010000224         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1307064,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000224 DASTFC010000000
VERSION     DASTFC010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307064"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VFO88_06670"
     CDS             complement(<1..1130)
                     /locus_tag="VFO88_06670"
                     /inference="COORDINATES: protein motif:HMM:NF036827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9545244.1"
                     /translation="MKTKRKQRRAKGTAHPELVGLGLLALGLFLGTVLLAGWDGGAVG
                     ERAETWLDAAIGGARIALPPFLVVVGGLMVARSELVDVRPFRTGIAIFSVGLLVLLGD
                     ERGGALGGLSDAVFGRLLGATGSFLLGAFLLSAGILLLTGASVGALLRRSAEAARAAH
                     DRAREARRAAIEAAAANRPPAPFAPEPAGPPVDGEKSFPDVVRDEEPLALAPALVDQL
                     AHEEPEALDDLDEPSEPSLFPDERPPLEHADYRLPDPSLLRKSRPSDGQPAKATERTA
                     EALVTALASHGVEATVIGQVSGPRVTRYELQLAPGTKVGKVSNLKDDLAYALATTEIR
                     ILAPIPGKQAVGVEVPNLAPNIVTLGDIFDTMPANASPVSVWL"
     gene            complement(1223..2161)
                     /locus_tag="VFO88_06675"
     CDS             complement(1223..2161)
                     /locus_tag="VFO88_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET9545245.1"
                     /translation="MSNAPTPIILDCDPGHDDAIALLLALASPEVELLGVTTVAGNQT
                     LAKTTANAIRTLEHVGREEILVAAGAERPLVRAQHAAPEVHGETGLDGPDLPPARGRP
                     LDAHAVDFIAERLAAFDRPVTLVPVGPLTNIGLLLARHPEAAERIERIVLMGGAIAEG
                     NVTPAAEFNVWADPEAAQRVFSSGLDVTMVGLDVTHAALLMPDRVEELRAAGRAGRFV
                     AELFDFFHRFHEQIYGFAGSPIHDAVAVAEVIRPGIVRTEHRHVEVDCASELCRGRTV
                     VDLWRRTGKEPNARVGVGIDAEAFVDLLVERISSLG"
     gene            complement(2158..3006)
                     /locus_tag="VFO88_06680"
     CDS             complement(2158..3006)
                     /locus_tag="VFO88_06680"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET9545246.1"
                     /translation="MAGPSVTVVGSCNLDLVAFCERLPRPGETLTASDFERVPGGKGA
                     NQAVAAARLGADVRFVGCIGDDEHGRLATAGLEEAGVDLSGLRRVDGEPTGLALIAVS
                     EGGDNQIVVVPGANRRLDAADVDASGADAVLTQLEIPLEAVARATETSTGLTALNAAP
                     ARDVPPETLARCDLIVANSFELDALGSSPLGPLFALTLGADGALLLEGGEEVARATPP
                     TVTAVDGTAAGDAFTACLVVSLAEGRPRDEALERACAAGAIAASRPGAQPSLPTAAEV
                     EAILAG"
     gene            complement(3082..3552)
                     /locus_tag="VFO88_06685"
     CDS             complement(3082..3552)
                     /locus_tag="VFO88_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545247.1"
                     /translation="MGRRVLVAALASGLAALALAVPAVGAPPTVERIEVDETTPDEFL
                     TEACGFPVTTHAKGHLVVIESGGEGTGVVAVRTLNIALTATANGKTYRFRDVGADQVR
                     RTPDGFEILMIIGQLPFTFTGVLKVNETTGEVILEPQHSTEGDVEDACAALAPA"
BASE COUNT          493 a         1377 c         1497 g          502 t
ORIGIN      
        1 agccagacgc tgaccgggct cgcgttcgcg ggcatcgtgt cgaagatgtc gccgagcgtg
       61 acgatgttcg gcgcgaggtt cggaacctcg acgccgaccg cctgcttgcc cgggatcggc
      121 gcgaggatgc ggatctccgt cgtggcgagc gcgtacgcga ggtcgtcctt caggttcgag
      181 accttgccga ccttcgtccc gggcgcgagc tggagctcgt agcgcgtgac gcggggcccc
      241 gagacctggc cgatcaccgt cgcctcgacg ccgtggctcg cgagcgccgt gacgagcgcc
      301 tcggcggtgc gctcggtcgc cttcgcgggc tggccgtcgg acgggcggga cttgcggagg
      361 agcgacgggt ccggcaggcg gtagtccgcg tgctcgagcg gcggccgctc gtccgggaag
      421 agcgacggct cgctcggctc gtcgaggtcg tcgagcgcct ccggctcctc gtgcgcgagc
      481 tggtcgacga gcgccggcgc gagcgcgagc ggctcctcgt cgcgcacgac gtcggggaag
      541 gacttctcgc cgtcgacggg ggggccggcg ggctcggggg cgaacggcgc gggcgggcgg
      601 ttcgccgccg cggcctcgat ggcggcgcgg cgcgcctcgc gggcccggtc gtgcgcggcg
      661 cgggcggcct ccgccgagcg ccgcaggagc gcgccgacgg acgcccccgt gaggaggagg
      721 atcccggccg agaggaggaa ggcgccgagc aggaacgacc ccgtcgcgcc gagcaggcgg
      781 ccgaagacgg cgtcggagag gcctccgagc gccccgcccc gctcgtcccc gagcaggacg
      841 aggaggccga cggagaagat ggcgatcccc gtgcggaacg gacggacgtc cacgagctcc
      901 gagcgggcga ccatgagccc gccgacgacg acgaggaacg gcgggagcgc gatgcgcgcg
      961 ccgccgatcg cggcgtcgag ccaggtctcg gcccgctcgc ccaccgcacc gccgtcccag
     1021 ccggcgagta ggacggtccc gaggaagagc ccgagcgcga gcagcccgag cccgacgagc
     1081 tcgggatgcg cggtgccctt cgcccgccgc tgcttccgct tggtcttcac gcgcgccggc
     1141 gagcggggac gcgtcttcgc gggcgtcttc ttccggccca tgcaggcgct attcgcgcgg
     1201 cggagcgccg ctcctgcccg gatcagccga gcgacgagat gcgctcgacg agcaggtcga
     1261 cgaaggcctc cgcgtcgatc ccgacgccga cccgcgcgtt cggctccttg cccgtccggc
     1321 gccagaggtc gacgaccgtg cggccgcggc agagctcgga cgcgcagtcg acctcgacgt
     1381 gacggtgctc ggtgcggacg atccccggac ggatgacctc ggcgaccgcg accgcgtcgt
     1441 ggatcggcga cccggcgaag ccgtagatct gctcgtggaa gcggtggaag aagtcgaaga
     1501 gctcggcgac gaagcgcccc gcgcggccgg cggcgcggag ctcctcgacg cggtccggca
     1561 tgaggagcgc cgcgtgcgtc acgtcgaggc cgaccatcgt cacgtcgagc ccgctcgaga
     1621 agacgcgctg cgcggcctcc gggtcggccc agacgttgaa ctccgcggcc ggcgtcacgt
     1681 tcccctcggc gatcgcgccg cccatgagga cgatgcgctc gatccgctcc gcggcctccg
     1741 ggtggcgcgc gagcaggagg ccgatgttcg tcagcgggcc gacggggacg agggtgaccg
     1801 ggcggtcgaa cgcggcgagg cgctcggcga tgaagtcgac ggcgtgggcg tcgagcggcc
     1861 gtccgcgcgc cggcgggagg tcggggccgt cgagccctgt ctcgccgtgc acctcgggag
     1921 cggcgtgctg ggcgcgcacg agcggccgct cggcgccggc cgcgacgagg atctcctccc
     1981 gtccgacgtg ctcgagcgtg cggatcgcgt tcgcggtcgt cttcgcgagc gtctggttcc
     2041 ccgcgacggt cgtcacgccg aggagctcca cctcggggct cgcgagcgcg agcaggagcg
     2101 cgatcgcgtc gtcgtggccc ggatcgcagt cgaggatgat cggtgtgggc gcgttgctca
     2161 tccggccagt atcgcctcca cttccgcggc ggtgggcagg gagggttgcg cgccggggcg
     2221 ggacgccgcg atcgcgccgg cggcgcacgc gcgctcgagc gcctcgtcgc gcgggcggcc
     2281 ctcggcgagc gagacgacga ggcaggcggt gaacgcgtcg ccggccgcgg tgccgtccac
     2341 ggcggtgacg gtcggcgggg tcgcacgggc gacctcctcc ccaccctcga ggaggagcgc
     2401 gccgtccgcg ccgagcgtga gggcgaagag cggcccgagc gggctcgagc cgagcgcgtc
     2461 gagctcgaag ctgttggcga cgatcaggtc gcagcgggcg agcgtctcgg gcgggacgtc
     2521 gcgcgccggc gcagcgttca gcgcggtgag cccggtggac gtctccgtcg cgcgggccac
     2581 ggcttcgagc gggatctcga gctgggtcag cacggcgtcg gcgccggacg cgtcgacgtc
     2641 cgccgcgtcg aggcggcggt tcgccccggg gacgacgacg atctggttgt cgccgccctc
     2701 ggagaccgcg atgagcgcga gcccggtcgg ctcgccgtcg acgcgccgca ggccgctcag
     2761 gtcgacgccc gcctcctcga gccccgccgt cgccaggcgt ccgtgctcgt cgtctccgat
     2821 gcagccgacg aagcggacgt cggcgccgag ccgcgccgcc gcgaccgcct ggttcgcgcc
     2881 cttcccgccc gggacgcgct cgaagtcgct cgccgtgagc gtctcccccg gccgcggcag
     2941 ccgctcgcag aacgcgacga ggtcgaggtt gcagcttccg acgacggtga cggacggccc
     3001 ggccacgcgg ccgattctgc ccggcggagg cggaggggcg ccggctgacc cggcgccccc
     3061 tccgcgagaa tgacggcctg cctatgcggg cgcgagcgcc gcgcaggcgt cctcgacgtc
     3121 gccttccgtg ctgtgctgcg gctcgagaat cacctctccg gtcgtctcgt tgaccttcag
     3181 gacccccgtg aaggtgaacg ggagctggcc gatgatcatg aggatctcga acccgtcggg
     3241 cgtccgtctc acctgatcgg cgccgacgtc ccggaagcgg taggtcttgc cgttcgccgt
     3301 cgcggtcagc gcgatgttca gcgtgcggac ggcgacgaca cctgtcccct cgccccccga
     3361 ctcgatcacg accaggtgcc ccttcgcgtg cgtcgtgacc gggaagccgc acgcctctgt
     3421 caggaactcg tcgggggttg tctcgtcgac ttcgatccgc tcgaccgtcg gcggtgcacc
     3481 gacggccggt accgcgagag ccagagcggc gagcccgctc gccagcgccg caaccagaac
     3541 ccgtctaccc attctccgct ccttccgtgg agatcgccgc tgcggcccgg cgatatgtgt
     3601 gggcggggga tatcagatcg cggcgatcgg cacaagcagc gggtttgtgc ggcgccgggc
     3661 gaccgcgacg acgcgagctc gaccagacgt cacgtgccag gggccttgac acgctggagc
     3721 ggccgtgaaa cggtcgcaac gattgtcacg ttcgctcggg ccgcagccgc ggacgtccgt
     3781 gtcgacgaca gggggagaat cgcatgaacg gacggagagt tatgggtctc gggacggtgg
     3841 tggccgcgac cgtctcactg gcgcttccg
//
LOCUS       DASTFC010000225         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_927817,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000225 DASTFC010000000
VERSION     DASTFC010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927817"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFO88_06690"
     CDS             complement(<1..425)
                     /locus_tag="VFO88_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET9545248.1"
                     /translation="MLELDFMRMAFAAGSVVGLLAPAVGFFVVQRRMSLIGDGIGHTA
                     FAGVAFGYLVGLSPVLTALVAAVGGAVAVEWLRARRGTAGDQALALLFYTGLAAGVVL
                     VSAAGRFDAGLFAFLFGAILTVTTSDLVLVGVLGAVGLAT"
     gene            complement(418..1137)
                     /locus_tag="VFO88_06695"
     CDS             complement(418..1137)
                     /locus_tag="VFO88_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET9545249.1"
                     /translation="MTPAIELHGVDYAYDGGPPVLRDACLTVEEGEFVAIAGPNGGGK
                     TTLLRILLGLARPAAGTALLFGEPAHRFSRRATLGYLAQRSRLGTDAPATVREVVSAG
                     RLAAGGLLGPLRAGDKAIVARAIADVGLEEKADAPLATLSGGQQQRAFIAKALAAEPS
                     LLVLDEPTAGVDPGAQESLARLLERLHRERQVTVLYVSHEFGAVESVVRRLVLVRGGI
                     VFDGPPSDLPGAWHDPSHVHA"
     gene            complement(1134..1970)
                     /locus_tag="VFO88_06700"
     CDS             complement(1134..1970)
                     /locus_tag="VFO88_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET9545250.1"
                     /translation="MILILLLVLSATGCGGEDDGDAADGDSLEVVASFYPLAYAAERT
                     APEAEVLNVTPPGVEPHDLELAPQDVARIRGADVVLYLGGGFQPAIEDAAGGAEGRAV
                     DLLEELGLPADGDVHVWLDPARYADVVRRVATELTGDEGSARLTLHRLDELDGIYELG
                     LETCERREIVVSHAAYGFLADAYGLEEIPLARVPDAEATPRDLERIAQLVRDRGVTTV
                     FVEPLASSEEAETIARETGAEVAVLDPLEGLTEEQLAAGEDYFSVMLANLEALQAALD
                     CE"
BASE COUNT          307 a          786 c          829 g          234 t
ORIGIN      
        1 gtcgcgagcc cgaccgcgcc caggacgccg accagcacga ggtcgctcgt cgtcaccgtg
       61 aggatggcgc cgaagaggaa ggcgaagagc cccgcgtcga agcgcccggc cgcggagacg
      121 agcacgacgc ccgcggcgag cccggtgtag aagaggagcg cgagcgcctg gtcgccggcc
      181 gtcccgcggc gcgcgcgcag ccactcgacc gcgacggcgc cgccgaccgc ggctacgagc
      241 gccgtcaaga cgggcgagag gccgacgagg tagccgaacg cgacgccggc gaacgcggtg
      301 tggccgatcc cgtccccgat gaggctcatg cggcgctgga cgacgaagaa cccgaccgcg
      361 ggcgcgagga gcccgacgac cgagccggcc gcgaaggcca tccgcatgaa gtcgagctca
      421 agcatggacg tgcgacgggt cgtgccaggc gccgggcaga tcggagggcg gcccgtcgaa
      481 gacgatcccg ccgcggacga gcacgagccg gcgcacgacg ctctcgaccg cgccgaactc
      541 gtgcgagacg tagaggacgg tcacctgccg ctcgcggtgc agccgctcga ggagccgcgc
      601 gagcgactcc tgcgcgcccg ggtcgacgcc cgcggtcggc tcgtcgagga cgaggagcga
      661 cggctcggcg gcgagcgcct tcgcgatgaa ggcccgctgc tgctgaccgc ccgagagcgt
      721 cgcgaggggc gcgtccgcct tctcctcgag cccgacgtcg gcgatcgccc gggcgacgat
      781 cgccttgtcc ccggcacgga gcgggcccag gagcccgccg gcggcgagac ggcccgcgct
      841 cacgacctcg cggacggtgg cgggcgcgtc cgtcccgagg cggctgcgct gcgcgaggta
      901 cccgagcgtc gcacggcgcg agaagcggtg cgcgggctcc ccgaagagga gcgccgtgcc
      961 cgcggccggg cgggccaggc cgagaaggat gcgcaggagc gtcgtcttcc ccccgccgtt
     1021 cggcccggcg atcgcgacga actcgccctc ctcgaccgtg aggcacgcgt cgcgcaggac
     1081 gggcgggccg ccgtcgtagg cgtagtcgac gccgtggagc tcgatcgcgg gggtcattcg
     1141 cagtcgagcg cggcctggag cgcctcgagg ttcgcgagca tgacggagaa gtagtcctcg
     1201 cccgcggcga gctgctcctc ggtgaggccc tcgagcgggt cgagcacggc cacctcggcg
     1261 cccgtctcgc gggcgatcgt ctcggcctcc tcggaggagg cgagcggctc gacgaagacg
     1321 gtcgtcaccc cgcggtcgcg gacgagctgc gcgatccgct cgaggtcgcg cggcgtcgcc
     1381 tcggcgtccg gcacccgggc gagcgggatc tcctcgagcc cgtaggcgtc ggcgaggaac
     1441 ccgtaggcgg cgtggctgac gacgatctcc cgccgctcgc aggtctcgag gccgagctcg
     1501 tagatcccgt ccagctcgtc cagccggtgg agcgtcagcc gcgcggagcc ctcgtcgccc
     1561 gtcagctcgg tcgcgacacg gcggacgacg tccgcgtagc gggccgggtc gagccagacg
     1621 tggacatcac cgtctgccgg gaggccgagc tcctccagca ggtcgacggc gcgtccctcg
     1681 gccccgccgg cggcgtcctc gatcgccggc tggaagccgc cgccgagata gaggacgacg
     1741 tccgcgccgc ggatgcgggc gacgtcctgc ggggcgagct cgaggtcgtg cggctcgacg
     1801 ccgggcggcg tcacgttcag cacctcggct tccggcgccg tccgctccgc ggcgtacgcg
     1861 aggggataga acgacgcgac gacctcgagc gagtctccgt ccgcggcatc gccgtcgtcc
     1921 tctccgccgc agccggttgc cgagagcacc agcaagagga tgagaatcat tctcacttcg
     1981 tggtacccta gccgcgggat gagcgagggc gcaagcaccc tgctcgcggc ggcgggcctc
     2041 cgggcgacgc cgcagcggac ggcgatcgtc ggcgagctcg tgcgcgagcc gaacgacgtg
     2101 accgcccagg agctccacga ccggctgcgc gcccgcgggg gaagcgcgcc ggggct
//
LOCUS       DASTFC010000226         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_38154,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000226 DASTFC010000000
VERSION     DASTFC010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38154"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFO88_06705"
     CDS             <1..334
                     /locus_tag="VFO88_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9545251.1"
                     /translation="KYSPSGGRVELSVEAVDANVRFAVRDEGLGIPADEQERIFEKFY
                     RLDPAMTRGVGGTGLGLYICHELVRRMGGRIWVESTHGEGSTFSFELPAAVPAGPVAR
                     DQSAPARA"
     gene            complement(350..1654)
                     /locus_tag="VFO88_06710"
     CDS             complement(350..1654)
                     /locus_tag="VFO88_06710"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9545252.1"
                     /translation="MLSPPDTAASARPDARPAEALASGRFQVPAALSGVLRLGADEAD
                     RHDLAVRKALLVGVSLMVLPAGVAWGAVYWLLDERLAALVPWTYAVLDVVALGVFART
                     RSFRFLRNVQLTLILVLPALLVVALGGLSAASGAVLWSLLAPLGAVVFDRPARAWLWF
                     AAFLGVMVASIVVAPLARPEAADIPLDARSALAALNIAGVSLVAFTLLAAFARQRAAA
                     QARVERLLLNILPDEIAERLQDEPGTLADHFEDVSVLFADVVDFTPLSSRLHPTELVE
                     VLDALFTEFDRLADEHGVEKIKTIGDCYMVAAGVPSPRADHAHALAEMALEMHECAAR
                     CLADHEAGDLALRIGISSGPVVAGVIGRRRFLYDLWGDTVNMASRMESHGVPGRVHIT
                     RATWERIGGDFRCERRGEVDVKGKGLVETWHLVGRHGDGAAV"
     gene            complement(1761..>2378)
                     /locus_tag="VFO88_06715"
     CDS             complement(1761..>2378)
                     /locus_tag="VFO88_06715"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET9545253.1"
                     /translation="AAHVGLLDRVTELPVSADLENGYGAEPEAAAHAVTRAAEAGAVG
                     GSIEDWDPGAGIYELAHAVERVAASVEAARRLPFRFMLTARAENHIRDNPDLDDTIAR
                     LQAFEAAGADVLYAPGRATVEEMRAVCGAVSRPVNVLAVPGLTFDEVAAAGAQRVSVG
                     GSLAWSAVGAATAAALAIRTGGDFSGLGTSALFRDWLAPSRDAED"
BASE COUNT          324 a          873 c          909 g          272 t
ORIGIN      
        1 caagtactcg ccgagcggcg gccgcgtcga gctctcggtc gaggccgtcg acgcgaacgt
       61 gcgcttcgcc gtgcgcgacg aggggctcgg gatcccggcc gacgagcagg agcggatctt
      121 cgagaagttc taccgcctcg acccggcgat gacccgcggg gtcggcggca cgggcctcgg
      181 cctctacatc tgccacgagc tcgtccgccg gatgggcggg cggatctggg tcgagtcgac
      241 gcacggcgag ggctcgacgt tctcgttcga gctgccggcc gctgtgcccg ccggcccggt
      301 cgcgcgcgac cagtccgccc ccgcgcgggc ctgatctacg ggcgcaggct cagacggcgg
      361 cgccgtcgcc gtgccggccg acgaggtgcc acgtctcgac gaggcccttg cccttcacgt
      421 cgacctcgcc ccgccgctcg cagcggaagt ctccgccgat gcgctcccag gtcgcccgcg
      481 tgatgtggac gcggccgggg actccgtgcg actccatgcg gctcgccatg ttgacggtgt
      541 caccccagag gtcgtagagg aagcggcgcc tgccgatcac gccggcgacg accggcccgg
      601 agctgatccc gattcgcagc gccaggtcgc ccgcctcgtg gtcggccagg cagcgcgcgg
      661 cgcactcgtg catctcgagc gccatctccg cgagcgcgtg cgcgtgatcc gcccgcgggc
      721 tcgggacgcc cgcggcgacc atgtagcagt ccccgatcgt cttgatcttc tcgacgccgt
      781 gctcgtccgc gagccggtcg aactcggtga agagcgcgtc gaggacctcg acgagctcgg
      841 tcgggtggag gcggctcgag agcggcgtga agtcgaccac gtcggcgaag aggacgctca
      901 cgtcctcgaa gtggtcggcg agcgtgcccg gctcgtcctg gagccgctcg gcgatctcgt
      961 ccgggaggat gttgagcagc aggcgctcga cccgcgcctg cgccgccgca cgctggcgcg
     1021 cgaaggcggc gaggagcgtg aacgcgacga gcgagacgcc cgcgatgttg agcgcggcga
     1081 gcgcgctgcg cgcgtcgagc gggatgtcgg cggcctccgg gcgggcgagc ggggcgacga
     1141 cgatgctcgc caccatgacg cccaggaacg cggcgaacca gagccacgcg cgcgccgggc
     1201 ggtcgaacac gaccgcgccg agcggcgcga ggagcgacca gagcacggcc ccgctcgcgg
     1261 cggagagccc gccgagcgcg acgacgagga gcgcaggcag gacgaggatc agggtcagct
     1321 ggacgttgcg gaggaaccgg aagctccgcg tgcgcgcgaa gacgcccagc gcgacgacgt
     1381 ccagcaccgc gtacgtccac gggaccaggg cggcgaggcg ctcgtcgagc agccagtaga
     1441 ccgcgcccca ggcgacgccc gcgggaagca ccatgagcga cacgccgacg agcagcgcct
     1501 tccgcacggc gaggtcgtga cggtcggcct cgtcggcccc gagccgcagg acgcccgaga
     1561 gcgccgccgg gacctggaac cggcccgacg cgagcgcctc cgcaggccgc gcgtccgggc
     1621 gggcggaagc tgcggtgtcc gggggggaga gcactgcttc ggccggaagt atcctccctg
     1681 cgagccgcgg ccgcacgacc cgttcgaaca ggccgcgcgc ccacggcgag cgtttcgggc
     1741 ctgggcctga cgctcgggct ctagtcttcc gcgtcccgcg agggcgcgag ccagtcgcgg
     1801 aagagcgcgc tcgtcccgag cccggagaag tcgccgccgg tgcggatcgc gagcgcggcg
     1861 gcggtcgccg cgccgacggc gctccacgcg agcgagccgc cgacgctcac ccgctgcgcg
     1921 cccgcggccg ccacctcgtc gaacgtgagc cccgggacgg cgaggacgtt cacgggccgc
     1981 gagacggccc cgcagacggc gcgcatctcc tcgacggtgg cacggccggg tgcgtagagg
     2041 acgtcggcgc cggccgcctc gaacgcctgg aggcgcgcga tcgtgtcgtc gaggtccggg
     2101 ttgtcgcgga tgtggttctc cgcccgggcc gtcagcatga agcggaacgg cagacggcgt
     2161 gcggcctcga ccgaggcggc gacgcgctcg accgcgtgcg cgagctcgta gatccccgcg
     2221 cccgggtccc agtcctcgat cgacccgccg accgcgccgg cctcggcggc gcgggtgacc
     2281 gcgtgcgcgg ccgcctcggg ctcggccccg tagccgttct cgaggtcggc cgagaccgga
     2341 agctccgtca cgcggtcgag gagcccgacg tgcgccgc
//
LOCUS       DASTFC010000227         7371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_70965,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000227 DASTFC010000000
VERSION     DASTFC010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70965"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFO88_06720"
     CDS             <1..202
                     /locus_tag="VFO88_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET9545254.1"
                     /translation="QYAYGSPVSKSALQAGDVVFFNGLGHNGIYIGGGQFVHAPHTGD
                     VVKISNLNEGWYASTSVGARRM"
     gene            395..1249
                     /locus_tag="VFO88_06725"
     CDS             395..1249
                     /locus_tag="VFO88_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9545255.1"
                     /translation="MRTIWNGSISFGLVTIPIGLAVAQQRKDVAFRTLHRECGTPIKQ
                     KRWCPTHERDVEADELVKGWEFTKGQFVVVEDEDFEAVALTRSQSIDIMSFVPVAEVD
                     PIYFDRTYYLAPADADAQRKPYVLLLEAMKQTGLGAVGKFVLWGKENLCLIRPLGESL
                     ALETLYFAEDIRSRAEIDEAVEETSVADAELAMAVQLVESMQGAYDPADYGNDYREAL
                     RAMLEAKLAGEEITAPEPAPAAPVIDLMEALKQSVEQAAGKRDGAKKPAARRKAASGG
                     RKRAASRS"
     gene            complement(1289..1468)
                     /locus_tag="VFO88_06730"
     CDS             complement(1289..1468)
                     /locus_tag="VFO88_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545256.1"
                     /translation="MANHFTPEELSEELGIPREEIIRVCVQESIPIYHGKIDKSLFQA
                     QLAASGGSTGRSEAA"
     gene            complement(1638..2381)
                     /locus_tag="VFO88_06735"
     CDS             complement(1638..2381)
                     /locus_tag="VFO88_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET9545257.1"
                     /translation="MAQRDEIIAFCDELLDVGAYPDYGPVGLQVVGAAEVRKVVCGVS
                     ASRELFERAAEAEAQLVLVHHGLFWEKESRVVDRIVRGRLQALFEADLTLLGYHLALD
                     AHPEIGNNAYLCRELGVEPERRFTDWGWGGSLAAPVPASDFANRVQEVVGRMPLVFSY
                     GPEEIRRVAICSGGAGRYLGTAIAEGYDCFLTGEADEPTKHLAKEAGVHFVAAGHYAT
                     ETSGVQALAARVAERFDVEWEFVDLPNPV"
     gene            2403..2476
                     /locus_tag="VFO88_06740"
     tRNA            2403..2476
                     /locus_tag="VFO88_06740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2437..2439,aa:Pro,seq:cgg)
     gene            complement(2528..3988)
                     /locus_tag="VFO88_06745"
     CDS             complement(2528..3988)
                     /locus_tag="VFO88_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545258.1"
                     /translation="MRKTLIAATVTAVALVFGAVALTAPSNGDELLKDEKHFLEQSNK
                     TFSGGSKAGSARNMVVVGKNDLGLRGFNADVWAHEGYAYVGQWGFQDWTNGSKTRFCP
                     SGDRAGVAVLDARNPANPTVVSRLLNPAGTSAEDVVVYTAPYGPLAGRDIAAVGIQTC
                     GGSRYDESFRRGIQLFDVTSPATPVEVGFLDSGCCTRGVHELEFAHRADLGRTFVYAS
                     VPTSEYDDALSPSGRRDQQGRGDFRLVDVTNPAAPVEVSNWGVLRNLGAAPTPGQGCD
                     ADGVYGHSAMPSHDGKLAFVSYWDSGFIALDVTNPANPVYKGRTVYEADEDGDAHSSM
                     YDMERKLLFTADEDFCKTSGPGIETGYGYLRVYDYSNLAAPVQIGEFRTQNSLGIGAQ
                     GSGDYTIHNAFVHGTDVYISWYSDGIRVVDASNPRAPREVAHFVPPAGQNTVKPSQRG
                     TLSNTTQVWGVYVDEATGLVYASDMNTGLWILRRTG"
     gene            complement(4099..4500)
                     /locus_tag="VFO88_06750"
     CDS             complement(4099..4500)
                     /locus_tag="VFO88_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9545259.1"
                     /translation="MPRTPAEILLGAGTIALVGASPRPQRPSNSVMRYLLDAGYRVIP
                     VRPHVREVHGIPCVASLAEIDEPIDLVDVFRRADACPGVTQEAVDAGAKAVWLQLGLV
                     SPEARRIAEDAGVEYVENACTAIVHERAARA"
     gene            4524..5369
                     /locus_tag="VFO88_06755"
     CDS             4524..5369
                     /locus_tag="VFO88_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET9545260.1"
                     /translation="MSDRLRIACVQMCAGPVKADNLATAERLVGEAASAGARLVVLPE
                     KWNAWGSPDVLRANAEPLEGGETVAALAGWARRHGVAIVGGSFTEAREGRERLSNTCP
                     VLDEDGELVAAYRKIHMFDVDVGGVAYRESEAEEPGDETVVCAAGGWRVGLSVCYDLR
                     FPELYRILALDGAELVTVPAAFTAFTGKDHWELLVRARAVENQYYVAAANQWGTYGGG
                     TKASHGRSLVADPWGVVLAQAPDEDCVIVAELDRERLERIRAELPSLANRQAAAYRWP
                     DPARV"
     gene            5362..6057
                     /locus_tag="VFO88_06760"
     CDS             5362..6057
                     /locus_tag="VFO88_06760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9545261.1"
                     /translation="MSEPRPLEAVLFDVDFTLAKPGPLLGGPGYREAAARFGLDLDPA
                     RYLEARAAAIVDIEHHPELDHDEEVWVRFTEDILRGMGGDGPNIREIALEITRGWQRS
                     DNFELYEDALPTMAELRAHGLRIGLVSNTSRDLEDFIAHFRLDVDAWISSGRHGKVKP
                     SPSIFLAALELLGTVPERAAMVGDSPTDDVDGARALGLRAFLLDREGRFGGRDDALSD
                     LRALPAALGLVAG"
     gene            complement(6054..6581)
                     /locus_tag="VFO88_06765"
     CDS             complement(6054..6581)
                     /locus_tag="VFO88_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545262.1"
                     /translation="MKARRPGRVMQHMVAGAAVLAGALALAPGAWAPPPAEGTLVPGV
                     SLGGIELGMTRDEVRAAWGERFGVCRNCRRPTWFFNYRPYEPQGTGVSFRRGEVVHVF
                     TVWSPPGWQATDGLTLGADEIDVGAGLVLAGDRACDGYTAKRAVTGETETVFYVHEGK
                     LWGFGLVRPGANPCL"
     gene            6661..7107
                     /locus_tag="VFO88_06770"
     CDS             6661..7107
                     /locus_tag="VFO88_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9545263.1"
                     /translation="MNIHTDDWDVERETVKIRFVGHALGTALIGGVVLELAPGHRGPY
                     HLHHGNEELALVLAGAVTIRGEHDERTLGPGELAFFARGRDGLHALENRSQEPARVLL
                     LSSKVSPDVTERPEEGLVGAFAGDVPTLGRDAPVEHFFPVESSVRG"
     gene            complement(7111..>7371)
                     /locus_tag="VFO88_06775"
     CDS             complement(7111..>7371)
                     /locus_tag="VFO88_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9545264.1"
                     /translation="AKWHATESAFEAAHLAIQVHGAYGYSAEYGVERYFRNARAPIIY
                     EGTTQIHKMMQAEHALGYRNLNGSGGDVSPIASWAPALLARG"
BASE COUNT         1031 a         2670 c         2624 g         1046 t
ORIGIN      
        1 gcagtacgcg tacggctccc cggtctcgaa gtcggcgctc caggccggcg acgtcgtctt
       61 cttcaacggg ctcggccaca acgggatcta catcggcggc ggccagttcg tccacgcgcc
      121 gcacacgggc gacgttgtga aaatctcgaa cctgaacgag ggctggtacg cgagcacgtc
      181 cgtcggcgcc cgccggatgt agctccacgc acgcgggtaa cgggggcccg ccccctcccc
      241 tcacttccag cgtatcgagg aaaagcgcac gcgtctgtga cggttcgtgc cggtcttgtg
      301 ccggttcgtc cacaggccgg cggagcgccg cgggccgtgg cagggaaggg cgatcgcgcg
      361 acgaacgttg tctcccccgg tacgctctca tctcatgcgc acgatctgga acggctccat
      421 cagcttcggc ctcgtcacga tcccgatcgg gctcgcggtg gcccagcagc gcaaggacgt
      481 cgccttccgc acgctccacc gggagtgcgg cacgccgatc aagcagaagc gctggtgccc
      541 gacgcacgag cgcgatgtcg aggcggacga gctcgtcaag ggctgggagt tcacgaaggg
      601 ccagttcgtg gtcgtcgagg acgaggactt cgaggcggtc gcgctcaccc gctcgcagtc
      661 gatcgacatc atgagcttcg tccccgtcgc cgaggtcgac ccgatctact tcgaccggac
      721 gtactacctc gccccggccg acgcggacgc gcagcgcaag ccgtacgtgc tcctgctcga
      781 ggcgatgaag cagaccggcc tcggcgcggt cggcaagttc gtcctgtggg gcaaggagaa
      841 cctctgcctc atccgcccgc tcggcgagtc gctcgcgctc gagacgctct acttcgcgga
      901 ggacatccgc tcccgcgccg agatcgacga ggcggtcgag gagacgtccg tcgcggacgc
      961 ggagctcgcg atggccgtcc agctcgtcga gagcatgcag ggcgcctacg acccggccga
     1021 ctacgggaac gactaccgcg aggccctccg cgcgatgctc gaggcgaagc tcgccggcga
     1081 ggagatcacg gcgccggagc ccgccccagc cgcgcccgtg atcgacctga tggaggcgct
     1141 caagcagagc gtcgagcagg cggccggcaa gcgcgacggc gcgaagaagc cggccgcgcg
     1201 caggaaggcc gcgtccggcg gccgcaagcg cgcggcgtca cgctcctagc cggcgagtcc
     1261 cgccgtcacg gcgggcgagc gtgccctcct aggccgcttc gctccttccg gtcgaaccgc
     1321 ccgacgccgc gagctgggcc tggaacaggc tcttgtcgat cttcccgtgg tagatcggga
     1381 tcgactcctg cacgcacacg cggatgatct cctcgcgcgg gatcccgagt tcctcggaca
     1441 gctcttccgg cgtgaagtgg ttcgccacgg aaacgctcct ttcgctaccg atcgagggcg
     1501 caaaaaaacc cgccgcgttc gcgcaggcgg gctgcttcgc aaccaccggt tttcaggtct
     1561 cggctcaaag gccggccacc tcgtttcgca cggtcccaga atacccacgg accgcggacg
     1621 gggcaatcgc ccgccgatca gacggggttc gggaggtcga cgaactccca ctcgacgtcg
     1681 aaccgctcgg cgacacgcgc ggcgagcgcc tggacaccgg acgtctcggt cgcgtagtgg
     1741 ccggccgcga cgaaatgcac cccggcctcc ttcgccaggt gcttcgtcgg ctcgtccgcc
     1801 tcgccggtca ggaagcagtc atagccctcc gcgatcgccg tgccgaggta gcggccggcg
     1861 ccgccggagc agatcgcgac ccggcggatc tcctccggcc cgtacgagaa gacgagcggc
     1921 atccgcccga cgacctcctg cacgcggttc gcgaagtcgg acgccgggac gggcgcggcg
     1981 agcgacccgc cccagcccca gtccgtgaac cgccgctccg gctcgacgcc cagctcgcgg
     2041 cagaggtacg cgttgttccc gatctccggg tgcgcgtcga gcgcgaggtg gtagccgagc
     2101 agggtcaggt cggcctcgaa gagcgcctgc agccgaccgc ggacgatgcg gtcgacgacg
     2161 cgcgactcct tctcccagaa gagcccgtgg tggacgagga cgagctgcgc ctcggcctcg
     2221 gccgcgcgct cgaagagctc gcgcgacgcc gagacgccgc agacgacctt ccgcacctcg
     2281 gccgccccga ccacctggag cccgaccggc ccgtagtcgg gatacgcgcc gacgtcgagg
     2341 agctcgtcgc agaacgcgat gatctcgtcc cgctgcgcca tttcgatacc ctattgccgc
     2401 ctcggggtgt ggcgcagcct ggtagcgcgc gccgttcggg tcggcgaggt ccccggttcg
     2461 aatccgggca ccccgatgcg aggcggggcg acgtccctcg ggacgccgcc ccgctccacg
     2521 tctcccccta gcccgtcctc cgcaggatcc agaggccggt gttcatgtcg ctcgcgtaga
     2581 cgagcccggt cgcctcgtcc acgtacacgc cccacacctg ggtggtgttc gagagcgtgc
     2641 cgcgctgcga cggcttgacc gtgttctggc cggccggcgg gacgaagtgc gcgacctcgc
     2701 gcggggcgcg cgggttcgac gcgtcgacga ccctgatccc gtccgagtac cacgagatgt
     2761 agacgtccgt cccgtgcacg aacgcgttgt ggatcgtgta gtcgcccgat ccctgcgcgc
     2821 cgatgccgag cgagttctgc gtgcggaact cgccgatctg cacgggcgcc gcgaggttcg
     2881 agtagtcgta gacacgcagg tagccgtacc cggtctcgat ccccgggccg gaggtcttgc
     2941 agaagtcctc gtcggcggtg aagaggagct tccgctccat gtcgtacatc gacgagtgag
     3001 cgtcgccgtc ctcgtcggcc tcgtagaccg ttcgcccctt gtagaccggg ttggccgggt
     3061 tcgtcacgtc gagggcgatg aacccgctgt cccagtacga gacgaacgcg agcttcccgt
     3121 cgtgcgaggg catcgcgctg tggccgtaga cgccgtccgc gtcgcagccc tggccgggcg
     3181 tcggcgcggc gccgaggttc cgcaggacgc cccagttcga gacctcgacc ggcgcggccg
     3241 gattggtcac gtcgactagg cggaagtcgc cgcggccctg ctggtcgcgc cgcccgctcg
     3301 ggctgagggc gtcgtcgtac tcactcgtcg ggacggacgc gtagacgaac gtcctcccga
     3361 ggtcggcgcg gtgggcgaac tcgagctcgt ggacgccgcg cgtgcagcag cccgagtcga
     3421 ggaagccgac ctcgaccggc gtggccgggc tcgtgacgtc gaagagctgg atcccgcggc
     3481 ggaagctctc gtcgtagcgc gagccgccgc acgtctggat cccgaccgcc gcgatgtcgc
     3541 ggccggccag cgggccgtac ggcgccgtgt agacgaccac gtcctcggcc gacgttcccg
     3601 ccgggttgag caggcgggaa acgaccgtcg gattcgccgg attgcgcgcg tcgagcaccg
     3661 ccacgcccgc cctgtcgccg ctcgggcaga accgcgtctt agagccgttc gtccagtcct
     3721 ggaaccccca ctggccgacg taggcgtagc cctcgtgcgc ccacacgtcc gcgttgaagc
     3781 cgcggaggcc caggtcgttc ttgccgacga cgaccatgtt gcgggccgag ccggccttgc
     3841 tgccgccgct gaacgtcttg ttcgactgct ccaggaagtg cttctcgtcc ttcaggagct
     3901 cgtcgccgtt gctcggcgcc gtgagcgcga cggcgccgaa gacgagcgca accgccgtca
     3961 cggtggccgc gatcagggtc ttccgcattg actcccctcc tcgggtcgcg tcccctcccg
     4021 ccgcgattgt cctcccgcgc cgagcggagc gcaagatcgc ccccgcggaa cccttacaag
     4081 taacactgtg tcacttgttc aggcccgcgc tgcgcgctcg tgcacgatcg ccgtgcacgc
     4141 gttctcgacg tactcgacgc ctgcgtcctc ggcgatccgg cgcgcctcgg gggagacgag
     4201 gcccagctgg agccagaccg ccttggcgcc cgcgtcgacc gcctcctgcg tcacccctgg
     4261 gcaggcgtcg gcgcggcgga agacgtccac gaggtcgatc ggctcgtcga tctccgcgag
     4321 cgaggcgacg cacgggatcc cgtggacctc ccggacgtgt gggcgcacgg ggatcacgcg
     4381 gtagccggcg tccagcaggt agcgcatgac cgagttcgac ggccgctgcg gccgcggcga
     4441 cgcgccgacg agcgcgatcg tcccggcgcc gagcaggatc tccgcgggcg tacggggcac
     4501 gggcgcaagg ctatcctccg tccatgtccg accggcttcg catcgcctgc gtccagatgt
     4561 gcgccgggcc cgtgaaggcg gacaacctcg cgaccgccga gcggctcgtc ggcgaggcgg
     4621 cctccgcggg cgcgcggctc gtggtcctgc cggagaaatg gaacgcctgg gggtcgcccg
     4681 acgttctgcg cgcgaacgcc gagccgctcg agggcggcga gacggtcgcg gcgctcgcgg
     4741 ggtgggcccg gcgccacggc gtcgcgatcg tgggcggctc gttcacggag gcgcgcgagg
     4801 gccgcgagcg gctctcgaac acgtgccccg tcctcgacga ggacggcgag ctcgtcgcgg
     4861 cctaccggaa gatccacatg ttcgacgtgg acgtcggcgg ggtcgcctat cgggagagcg
     4921 aggcggagga gccgggcgac gagaccgtcg tgtgcgccgc cggcggctgg cgcgtcgggc
     4981 tctccgtctg ctacgacctc cgcttcccgg agctgtaccg gatcctcgcg ctcgacgggg
     5041 ccgagctcgt caccgtcccg gccgcgttca cggcgttcac gggcaaggat cactgggagc
     5101 tcctcgtccg cgcgcgtgca gtggagaatc agtactacgt cgcggccgcg aaccagtggg
     5161 ggacgtacgg cggcggcacg aaggcgagcc acgggcgctc gctcgtcgcg gatccctggg
     5221 gcgtcgtcct cgcccaggcg cccgacgagg actgcgtgat cgtcgccgag ctcgaccgcg
     5281 agcggctgga gcggatccgc gccgagctgc cgtcgctcgc gaaccggcag gccgccgcgt
     5341 accggtggcc ggatcccgcg cgtgtctgag ccgcggccgc tcgaggcggt tctcttcgac
     5401 gtcgacttca ccctcgcgaa gccgggcccg ctgctcggcg ggccggggta ccgcgaggcg
     5461 gccgcgcgct tcggcctgga cctcgacccg gcgcgctacc tcgaggcgcg cgcggccgcg
     5521 atcgtggaca tcgagcacca cccggagctc gaccacgacg aggaggtctg ggtgcgcttc
     5581 accgaggaca tcctgcgcgg aatgggcggc gacggcccga acatccggga gatcgcgctc
     5641 gagatcacgc gcgggtggca gcggtcggac aacttcgagc tctacgagga cgcgttgccg
     5701 acgatggcgg agctgcgcgc gcacgggctc cggatcggcc tcgtctcgaa cacgagccgc
     5761 gacctcgagg acttcatcgc gcatttccgc ctcgacgtgg acgcatggat ctcctccggc
     5821 cggcacggga aggtgaagcc gagcccgtcg atcttcctcg ccgcgctcga gctgctcggc
     5881 accgtgcccg agcgggccgc gatggtcggc gactccccga ccgacgacgt ggacggggcg
     5941 cgcgcgctcg gcctgcgcgc gttcctgctc gaccgggagg ggcgcttcgg cgggcgtgac
     6001 gacgcgcttt cggacctccg cgcgctgccc gcggcgctcg ggctcgtcgc cggctaaagg
     6061 caggggttcg cgccgggccg gacgagcccg aagccccaga gcttcccctc gtggacgtag
     6121 aagaccgtct ccgtctcgcc cgtcacggcg cgcttcgccg tgtagccgtc gcaggcgcgg
     6181 tcgccggcga ggacgaggcc cgcgcccacg tcgatctcgt ccgcgccgag cgtgaggccg
     6241 tcggtcgcct gccacccggg cgggctccag acggtgaaga cgtgcacgac ctcgccgcgc
     6301 cggaaggaca cgcccgtgcc ctgcggctcg taggggcggt agttgaagaa ccaggtcggc
     6361 cggcggcagt tccggcacac gccgaagcgc tcgccccagg cggcgcgcac ctcgtcgcgc
     6421 gtcatcccga gctcgatccc gccgagcgag acccccggga cgagcgtccc ctccgcgggc
     6481 ggcggcgccc acgcgcccgg cgcgagggcc agcgcgcccg cgagaaccgc cgctcccgcg
     6541 accatgtgtt gcatcacccg gccgggccgt cgcgccttca cacgttgatg atgcaacacg
     6601 agccgggaaa ggcgcgcggc cgtcggtcgt tctcccgtgc aggcgaggga aggaggcgcg
     6661 gtgaacatcc acacggacga ctgggacgtc gagcgggaga cggtgaagat ccggttcgtc
     6721 gggcacgcgc tcgggacggc gctgatcggc ggcgtcgtcc tcgagctcgc gccgggacac
     6781 cgggggccgt accacctgca ccacgggaac gaggagctcg cgctcgtcct cgcgggcgcg
     6841 gtgaccatcc gcggggagca cgacgagcgc acgctcgggc ccggcgagct cgcgttcttc
     6901 gcgcgcggcc gggacgggct ccacgcgctc gagaaccgct cgcaggagcc ggcgcgcgtc
     6961 ctgctgctct cgtcgaaggt ctcgccggac gtcaccgagc gccccgagga gggcctcgtc
     7021 ggcgcgttcg cgggcgacgt gccgacgctc gggcgcgacg cgccggtcga gcacttcttc
     7081 ccggtcgaga gctcggttcg gggctgagcg ctagccgcgc gcgaggagcg ccggcgccca
     7141 ggacgcgatc ggcgacacgt cgccgccgga gccgttcagg ttccggtatc cgagcgcgtg
     7201 ctcggcctgc atcatcttgt ggatctgcgt cgtgccctcg tagatgatcg gggcgcgcgc
     7261 gttgcggaag taccgctcga cgccgtactc cgcggagtag ccgtacgcgc cgtgaacctg
     7321 gatcgcgagg tgcgccgcct cgaacgcgct ctcggtcgcg tgccacttcg c
//
LOCUS       DASTFC010000228         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_483260,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000228 DASTFC010000000
VERSION     DASTFC010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483260"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..78)
                     /locus_tag="VFO88_06780"
     tRNA            complement(5..78)
                     /locus_tag="VFO88_06780"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Asp,seq:gtc)
     gene            124..708
                     /locus_tag="VFO88_06785"
     CDS             124..708
                     /locus_tag="VFO88_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545265.1"
                     /translation="MANDPRTYQMAPAGAPATGLEEYAVETAGGEAVGKVQTLLEGDD
                     ELLLAVELGIPPLTHQVRVVPWERVEEVDHDALAVRLTLGKDELERAPELDPDRGVET
                     EDAELRRVETLPAEARGNVLPGNVAGPVDRLVLGPSIALGLLGVFAFLAWVIMVTQVR
                     DLTWHWALLAVPILLVGVSGYLAYDAFRNQADSR"
     gene            724..1017
                     /locus_tag="VFO88_06790"
     CDS             724..1017
                     /locus_tag="VFO88_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET9545266.1"
                     /translation="MTAAPETPTRKRPSDGSGGTGDAWRVIVLNDDHNTFDGVAFALA
                     RVLPGVTFERGLALADRIHTKGLAVVWTGHREQAELYWAQLEAFGLTMAPLER"
     gene            1366..>2647
                     /locus_tag="VFO88_06795"
     CDS             1366..>2647
                     /locus_tag="VFO88_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545267.1"
                     /translation="MNPLWRAAPLVLRRYPGVLAALVAAAALLTLAAAAGPLFVSASA
                     SAAVGDELRRLTPFGAGAYVRVTGPVAPEQVEAFGDPTFAERTAALGEATEDLPGRGG
                     IVATILGPELDATGPADGAGADLEVRLLARTGALEHVRLLEGGDGPGLWLADTVAEQL
                     GLEPGDRVLLGPDASGGTVSVPVAGLYRSLANEPRTPFWRSLESQIHPAPPAYAAQPT
                     FAIGDVETVTETATRLGLERADFRWELPLEPRSLSLAEAERVARGLEGLGAALGDERD
                     PLFELFVCRRCFRGIRAEFSTGLPRAVDAARASAATVRGPVDLLANAGSLVALAVVGA
                     VGAFALARRRVEVAALVARGTSPGSAGARLALEALLPVLAGAVLGVGAAYLLVTVFGP
                     DGSVDAEGLGDAVRAAALRVPVALALLALVGAAWT"
BASE COUNT          301 a          980 c         1005 g          361 t
ORIGIN      
        1 gagacgggag cgacgggact cgaacccgcg acctccggcg tgacaggccg gcgttctaac
       61 caactgaact acgcccccag gcgccggggc attgtatcgg cggccggtgg gtatcggccg
      121 gacgtggcga acgatcctcg cacgtaccag atggcgcccg cgggggcgcc ggcgaccggg
      181 ctcgaggagt acgcggtcga gacggccggg ggcgaggcgg tcggcaaggt tcagacgctc
      241 ctcgagggcg acgacgagct cctcctcgcg gtcgagctcg gaattccgcc gctcacgcac
      301 caggtccgcg tcgttccgtg ggagcgggtg gaggaggtcg accacgacgc gctcgccgtg
      361 cggctgacgc tcggcaagga cgagctcgag cgcgcccccg agctcgaccc ggaccgcgga
      421 gtggagacgg aggacgccga gctccggcgg gtcgagacgc tgccggccga ggcgcgcggg
      481 aacgtcctgc ccgggaacgt cgcggggccg gtcgacaggc tcgtgctcgg gccgtcgatc
      541 gcgctcgggc tgctcggcgt cttcgcgttc ctcgcctggg tgatcatggt cacgcaggtt
      601 cgcgacctga cgtggcactg ggccctcctc gcggtgccga tcctgctcgt cggcgtctcc
      661 ggctacctcg cgtacgacgc gttccgaaac caggcggaca gtcgctagct atcgtcgcgg
      721 gtcgtgaccg cggcgcccga gactccgacc cggaagcggc cgagcgacgg ctccggcggc
      781 accggcgacg cgtggcgcgt gatcgtcctg aacgacgacc acaacacgtt cgacggcgtc
      841 gccttcgcgc tcgcgcgggt gctccccggc gtgacgttcg agcgcggcct cgccctcgcc
      901 gaccggatcc acacgaaggg cctcgccgtc gtctggacgg gccaccgcga gcaggccgag
      961 ctctactggg cgcagctcga ggcgttcggc ctcacgatgg cgccgctcga gcgctgaccc
     1021 ggcggggaca caggacgggt accgaccacc gtggctttaa ggggagaggc cggagtcgga
     1081 gcggagacgc ctctcagcac gcgatagtcg atacccgttg cgcgcaactt ttcccgtgtt
     1141 ttcgcgcttg aaacgcttcg cggacaccgg tcggggccgg ggcacgtgcc cggccccgta
     1201 ggcgcttgcc ctcgaaggct gaggccgccg ccgaggagat cccctcgctc tgacacaccg
     1261 cggtgctcgt ggcccatacg ggagcggctg cccgacggat tccctaacga acgagcgcgg
     1321 cgcctcgtct gaacgaagcg tggctcgttc cggggtgcgc gcggcgtgaa tccgctctgg
     1381 cgggcggccc cgctcgtcct gcggaggtat ccgggagtgc tcgccgcgct cgtcgccgcg
     1441 gccgcgctcc tcacgctcgc ggccgccgcc gggccgctct tcgtctccgc gtccgcgagc
     1501 gccgcggtcg gggacgagct gcgccggctg acgccgttcg gcgcgggcgc gtacgttcgc
     1561 gtgaccgggc ccgtcgcgcc cgagcaggtc gaggccttcg gcgacccgac gttcgccgag
     1621 cggacggcgg cgctcggcga agcgaccgag gacctgccgg gccggggcgg gatcgtggcg
     1681 acgatcctcg ggcccgagct cgacgccacc ggcccggcgg acggcgccgg cgccgatctc
     1741 gaggtgcgcc tcctcgccag gaccggcgcc ctcgagcacg tccgcctgct cgagggcggt
     1801 gacggcccgg gcctctggct cgcggacacc gtcgccgagc agctcggcct cgagcccggc
     1861 gaccgcgtcc tgctcggccc ggatgcgagc ggcggcacgg tttcggtgcc ggtcgcgggc
     1921 ctctaccggt cgcttgcaaa cgagccgcgg acgccgttct ggcgctcgct cgagagccag
     1981 atccatccgg cgccgcctgc ctacgcggcg cagccgacgt tcgcgatcgg cgacgtcgag
     2041 accgtcacgg agacggcgac gcggctcggg ctcgagcgcg cggacttccg ctgggagctc
     2101 ccgctcgagc cccgctcgct ctcgctcgcc gaggccgagc gggtcgcgcg cgggctcgag
     2161 gggctgggcg ccgcgctcgg cgacgagcgc gacccgctct tcgagctctt cgtctgccgc
     2221 cgctgcttcc gtgggatccg cgcggagttc tcgaccgggc tgccccgcgc ggtcgacgct
     2281 gcgcgggcgt cggccgcgac cgtccgcggg cccgtcgacc tgctcgccaa cgccggctcg
     2341 ctcgtcgcgc tcgcggtcgt cggcgcggtc ggcgccttcg cgctcgcgcg gcggcgcgtg
     2401 gaggtcgcag ccctcgtcgc ccgcggaacg agccctgggt cggcgggcgc gcggctcgcg
     2461 ctcgaagcgc tcctgccggt cctcgcgggc gcggtgctcg gcgtcggagc cgcgtacctc
     2521 ctcgtgaccg tcttcgggcc cgacgggagc gtcgacgcag aggggctcgg cgacgcggta
     2581 cgggcggcgg cgctgcgcgt gccggtcgcg ctcgcgctcc tcgcgctcgt cggagccgcg
     2641 tggacgg
//
LOCUS       DASTFC010000229         4515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_400655,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000229 DASTFC010000000
VERSION     DASTFC010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400655"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..2421)
                     /locus_tag="VFO88_06800"
     CDS             complement(76..2421)
                     /locus_tag="VFO88_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9545268.1"
                     /translation="METRVATPAPAPAGLTEAEAARRLAERGPIEPPATSRSVASIVR
                     ANVFTVFNAILVVFGALMLVFGDWRDALFLGIVIANSGIGIGQELRAKRALDRLYAEI
                     APHATVVRDGEPREIEAGQVVVGDLVRAEAGDGIVADGTLVSSEGLALDESILTGESR
                     PVLRAAGEEVRSGSYAVEGAGLYEVTAVGHESYAQRIAGEAREFRHPRSPLERALNRL
                     LLLLVAFMLPLGTIFLYALWRRDEPFREALTTATAGIVTLIPEGLILLASLTFAAAAL
                     GLARRGALAQQLNAIESLASVDVVCLDKTGTLTEARLRVVSTEPADDVAEDVLRSALG
                     RFAASSPAQNRTLEALAEELGAEPEEPGAYVPFSSARRWSALALDGTSYVFGAPERLR
                     IGGRLAEIAEREQEAGRRVVAFGETWSSLVPGDRPPDGVRPLGLAVLAEELRPQTRET
                     VAFLRAEGVELRVVSGDAPATVGAIADDAGIEVGDGPVDGSATDDLPLDARVVGRVSP
                     EGKRRLVERLRADGRYVAMIGDGVNDVPALKASRLAIAQGSGSEMARSVADVVLVRGD
                     FDAFPRMVAEGRKILRNVQRVAKLFVTKSVFAAFIILAVGLTETPYPFLPRHLSLAAS
                     LTVGIPGFFLALAPSVGPWRSDGFLRDIARFAIPAGTMAGLGVVAAYLFALNVVGLGV
                     EEARTAAVSTLVAVGLYFVLALEAATRARAMAVGLLCLVLLALYVVVLAVEPLRGFFA
                     LVVPGVWAWVATLGGASLTVGALVLMDERFVPDEVRRRIAR"
     gene            2480..2902
                     /locus_tag="VFO88_06805"
     CDS             2480..2902
                     /locus_tag="VFO88_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET9545269.1"
                     /translation="MEIHPDARAILESDALAHLVTRNPDGSPQVSCVWVGLEGDEIVS
                     GHLVATQRKLRNVREDPRVVMSVEGTRIHPPGLREYLVVHGRARIEEGGGADLLERLA
                     RTYLGPDVKFPPMDDPPPGFVLRTTVERLGGIGPWAAR"
     gene            2930..3289
                     /locus_tag="VFO88_06810"
     CDS             2930..3289
                     /locus_tag="VFO88_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HET9545270.1"
                     /translation="MALTIFDRYGGFATVRKIVSAFYDKVLDSPLLVHHFENVDMRRL
                     VDHQARFVSSVMGGPASYSDNHLRRIHERHRITTEEFREMVDLLEETLEDFDFAEADV
                     REVIGQVRRREGVVVSA"
     gene            3286..4347
                     /locus_tag="VFO88_06815"
     CDS             3286..4347
                     /locus_tag="VFO88_06815"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9545271.1"
                     /translation="MTERVQELAPGVLKHLLQGLGTGVALIRPEDWTIAFENAAFFEW
                     FPPDAEVDDALAARASTLDEDKARARLEKGRPYTFETDAGSGARAVGLSVELRRCELG
                     GEEYLVLEAREITKQREAEYLLDSYSKMVERNTRDLQREKERVEKLLLNVMPRSVYEE
                     MKDFGSTTPQRFDAATILMLDFVGFTSMAISRDPSALIAELNDIFTSFDRIVELFGCE
                     RMKTIGDAYMAVAGLPDETPDHAHNMARVALRMRRYLERRNASHPEQWLCRIGIHSGP
                     VIGSIVGVQKYVYDIFGPGVNLASRLEAVAEPMQILASEATYGLIRDDFSLVERGEVD
                     LKGFDAQRLYSLEGELQRR"
BASE COUNT          663 a         1668 c         1602 g          582 t
ORIGIN      
        1 cgcaggcgct cttgctccgt cctcacgcgg ctgattatga cgacggctct cgcggaaatg
       61 ccgagccgcc cgcttctagc gcgcgatccg gcggcgcacc tcgtccggca cgaaccgctc
      121 gtccatgagc acgagcgcgc cgaccgtcag gctcgcgccg ccgagcgtcg ccacccaggc
      181 ccagacgccg gggacgacga gcgcgaagaa tccccgcagc ggctcgaccg cgagcacgac
      241 gacgtacagc gcgaggagga cgaggcagag cagcccgacc gccatcgccc gcgcccgcgt
      301 cgccgcctcg agcgcgagga cgaagtagag cccgacggcc acgagcgtcg agaccgccgc
      361 cgtccgcgcc tcctcgacgc cgagcccgac gacgttcagg gcgaagagat acgccgcgac
      421 gacgccgagg cccgccatcg tccccgccgg gatggcgaag cgcgcgatgt cgcggaggaa
      481 gccgtcgctc cgccacggcc cgacgctcgg agcgagcgcg aggaagaacc cggggatccc
      541 gaccgtgagc gacgccgcga ggctcaggtg gcgggggagg aacgggtacg gcgtctccgt
      601 caggccgacc gcgaggatga tgaacgcggc gaagacgctc ttcgtcacga agagcttcgc
      661 cacgcgctgg acgttccgga ggatcttgcg cccctcggcg accatgcgcg ggaaggcgtc
      721 gaagtcgccg cggacgagca cgacgtcggc gacgctccga gccatctccg agccgctccc
      781 ctgggcgatc gcgagccgtg acgccttgag cgcgggaacg tcgttcacgc cgtcgccgat
      841 catcgcgacg tagcgcccgt ccgcgcggag gcgctccacg aggcgccgct ttccctcggg
      901 cgacacgcgg cccacgacgc gcgcgtcgag agggaggtcg tcggtcgcgc tcccgtccac
      961 cggcccgtcg ccgacctcga tcccggcgtc gtccgcgatg gcgcccaccg tggcgggcgc
     1021 gtcgcccgac acgacgcgga gctccacccc ctcggcgcgg aggaaggcga cggtctcgcg
     1081 cgtttgcggg cgcagctcct cggcgagcac cgcgagcccg agcgggcgga cgccgtcggg
     1141 cggccggtcg ccggggacga gggaggacca ggtctcgccg aacgcgacga cgcggcggcc
     1201 cgcctcctgc tcgcgctcgg cgatctcggc gagcctgccg ccgatccgga gccgctcggg
     1261 cgcgccgaag acgtagctcg tcccgtcgag cgcgagcgcg ctccagcgcc gcgcggagga
     1321 gaacgggacg tacgcgcccg gctcctccgg ctcggcgccg agctcctcgg cgagcgcctc
     1381 gagcgtccgg ttctgcgccg gcgagctcgc ggcgaagcgc ccgagcgccg agcggagcac
     1441 gtcctcggcg acgtcgtcgg ccggctcggt cgagacgacg cgcaggcggg cctccgtgag
     1501 cgtcccggtc ttgtcgaggc aaacgacgtc cacggacgca agcgactcga tcgcgttcag
     1561 ctgctgggcg agcgcgccgc gccgcgccag cccgagcgcc gccgcggcga acgtgagcga
     1621 cgcgaggagg atgaggccct cggggatgag cgtgacgatc ccggccgtcg cggtcgtgag
     1681 cgcctcccgg aacggctcgt cgcgccgcca gagcgcgtag aggaagatcg tcccgagcgg
     1741 gagcatgaag gcgacgagca ggagcaggag ccggttgagc gcacgctcga gcggcgagcg
     1801 cggatgccgg aactcgcgcg cctcgcccgc gatccgctgc gcgtagctct cgtggccgac
     1861 cgcggtcacc tcgtagagcc ccgcgccctc gaccgcgtac gagcccgagc gcacctcctc
     1921 gccggccgcg cgcaggacgg gccgggactc gccggtcagg atcgactcgt cgagcgcgag
     1981 accctcggac gagacgagcg tcccgtccgc gacgatcccg tcgcccgcct cggcgcggac
     2041 gaggtcgccc acgacgacct gcccggcctc gatctcgcgc ggctcgccgt cgcggacgac
     2101 ggtcgcgtgc ggcgcgatct ccgcgtagag ccggtcgagc gcgcgcttcg cgcggagctc
     2161 ctgcccgatc ccgatccccg agttggcaat cacgatcccg aggaagagcg cgtcgcgcca
     2221 gtcgccgaac acgagcatga gcgcgccgaa cacgacgagg atcgcgttga agacggtgaa
     2281 gacgttcgcg cggacgatgc tcgcgacgga gcggctcgtc gccggcggct cgatggggcc
     2341 gcgctcggcc aggcggcggg ccgcctccgc ctccgtcagg ccggccggcg ccggagcggg
     2401 cgtcgcgacc ctcgtctcca cgggcggatt ctcacacgcc cgcttgcgcc cgcgcgggcc
     2461 gctgcgccac actcgtgtcg tggagatcca cccggacgcc cgcgcgatcc tcgagtccga
     2521 cgcgctcgcg catctggtga cgcgcaatcc ggacggctcg ccgcaggtct cgtgcgtgtg
     2581 ggtggggctc gagggcgacg agatcgtctc cggccacctg gtcgcgacgc agcgcaagct
     2641 tcgcaacgtg cgcgaggacc cgcgcgtcgt catgtcggtc gagggcacgc gcatccatcc
     2701 gccgggcctg cgcgagtacc tcgtcgtcca cggccgcgcc cggatcgagg agggcggcgg
     2761 cgccgacctc ctggagcgcc tcgcccgcac gtacctcggc ccggacgtga agttcccgcc
     2821 gatggacgac ccgccgcccg gcttcgtcct gcggacgacg gtcgagcgcc tcggcggcat
     2881 cggcccctgg gccgcccgtt aggatttccc gcacttccag ggaggggcct tggcgctcac
     2941 gatcttcgac cgctacggcg gattcgcgac ggttcggaaa atcgtctccg ccttctacga
     3001 caaggtgctc gactcgccgc ttctcgtcca ccacttcgag aacgtggaca tgcggcggct
     3061 cgtcgaccac caggcgcgct tcgtctcgtc cgtcatgggc ggccccgcga gctactcgga
     3121 caaccacctc cgccgcatcc acgagcgcca ccggatcacg acggaggagt tccgcgagat
     3181 ggtcgacctg ctcgaggaga cgctcgagga cttcgacttc gccgaggcgg acgtccggga
     3241 ggtgatcggc caggtccggc ggcgcgaggg cgtggtcgtc tccgcgtgac cgagcgcgtg
     3301 caggagctcg ctcccggcgt tctcaagcac cttctccagg ggctcgggac cggggtcgcg
     3361 ctcatccggc ccgaggactg gacgatcgcg ttcgagaacg cggcgttctt cgagtggttc
     3421 ccgcccgacg cggaggtgga cgacgcgctc gcggcccgcg cctccaccct cgacgaggac
     3481 aaggcccgcg cgcgcctcga gaagggccgt ccgtacacct tcgagacgga cgccggcagc
     3541 ggcgcccgcg cggtcggcct ctcggtcgag ctgcgccggt gcgagctcgg cggggaggag
     3601 tacctcgtcc tcgaggcgcg cgagatcacg aagcagcgcg aggccgagta cctgctcgac
     3661 tcctactcga agatggtcga gcggaacacg cgcgacctgc agcgcgagaa ggagcgcgtc
     3721 gagaagctcc tgctgaacgt catgccgcgc tccgtctacg aggagatgaa ggacttcggc
     3781 tcgacgaccc cgcagcgctt cgacgcggcg acgatcctca tgctcgactt cgtgggcttc
     3841 accagcatgg cgatctcccg cgacccgagc gcgctcatcg ccgagctgaa cgacatcttc
     3901 acgtccttcg accggatcgt cgagctcttc ggctgcgagc ggatgaagac gatcggggat
     3961 gcgtacatgg cggtcgcggg tctccccgac gagacgcccg accacgccca caacatggcg
     4021 cgcgtcgcgc ttcggatgcg ccgctacctc gagcggcgca acgcctccca ccccgagcag
     4081 tggctctgcc gaatcgggat ccactcgggc ccggtgatcg gctcgatcgt gggcgtgcag
     4141 aagtacgtct acgacatctt cgggccgggc gtgaacctcg cttcgcggct cgaggcggtc
     4201 gccgagccga tgcagatcct cgcgagcgag gcgacgtacg ggctcatccg ggacgacttc
     4261 tcgctcgtcg agcgcggcga ggtcgacctc aagggattcg acgcccagcg cctctactcg
     4321 ctcgagggcg agctgcagcg gcgttgagcg agagagggcg gccgaagccg ccctctctcg
     4381 tctcgcaggt tcgctcaggg ggctaccagc ggaccttcca gccgttcttc cagccgtccc
     4441 agaaccacgc ccacgggctc cacttccccc gcttgcggag ctcgagcgag aactcctggg
     4501 tctgcgggtt caggt
//
LOCUS       DASTFC010000230         5448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_54787,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000230 DASTFC010000000
VERSION     DASTFC010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54787"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VFO88_06820"
     CDS             <1..793
                     /locus_tag="VFO88_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9545272.1"
                     /translation="EQIVRPTGIVDPEVELRPTKNQIDDLLNEIRRREEAGERVLDTT
                     LTKKMAEDLTDYLLEAGVRARYLHSEIDTLERIAIIRELRLGEYDVLVGVNLLREGLD
                     LPEVSLVAILDADKEGFLRGQTALIQTIGRAARNVNGRVLMYADKQTEAIRGALEETN
                     RRREKQLAYNTEHGITPESIVKGVSDIAEFLSLESPNVPGRSRRRKASRVEGMSPSEV
                     EKLVVSLEEEMFAAAEELRFEYAAKLRDEIKELRRDLQEAAEAAA"
     gene            complement(797..1252)
                     /locus_tag="VFO88_06825"
     CDS             complement(797..1252)
                     /locus_tag="VFO88_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545273.1"
                     /translation="MRRFLPAALAAAFALVLAAPAAAEIVPQRSIAGVELSMTRAEVR
                     AVLGEPREIERGSNEFGRYIIFKYRRLQVVFQGRTNVTAVRTNRRNERTATGVGFGST
                     RAEVRAGVAGVRCNRRECVKGRFLPGRRVTIFRLYRGKVAQTVVGFVID"
     gene            complement(1353..3053)
                     /locus_tag="VFO88_06830"
     CDS             complement(1353..3053)
                     /locus_tag="VFO88_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HET9545274.1"
                     /translation="MTKGIRTAVAACVALVLPLVAASAMAAPGKKGPAPQAVAKAKTT
                     SVQLLAINDFHGNLEPPSGSSGRIGTVDAGGVEFLASHIDQLRATNPNTLVVSAGDLI
                     GASPLLSGLFHDEPTIEAANLFGLDFNAVGNHEFDEGATELLRMQEGGCHPVDGCQDG
                     TGFDGAEFDFLAANVEWEETGDTIFPPYKIVKFQGVKMALIGMTLEGTPEIVTPSGIA
                     GLEFHDEADTVNALVPELLKQNVKTIVVLVHEGGFPTGAYNECPGISGPIVDIVNRTS
                     DEVDLFVTGHTHQAYNCVIDGRPVSSAASFGRIVTDIDMTISRASKQPVEIRVNNNIV
                     TRDVARDPAQTELIERYRVVAAPIANRVVGSITADITRTNNTAGESSLGDHIADAQLA
                     ATSDPALGGAVVALMNPGGIRTDLVFNQISGGELPGQVTYGEIFAVQPFGNNLVTMTL
                     TGDQLERVLEQQWGPGTRQVILQISDSLRYTWDASAPIGDRIATSSITINGTPISPLA
                     TYRVTVNNFLADGGDGFTVLREGTNRLGGVVDTDAFADYLGANSPLSPTPRNRITRLN
                     "
     gene            complement(3144..3965)
                     /locus_tag="VFO88_06835"
     CDS             complement(3144..3965)
                     /locus_tag="VFO88_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9545275.1"
                     /translation="MPPLRVVQLSDLHAGTREEPEVEDALRTLVADLDPELVVASGDL
                     THRNRTEQHDRAARLLRSLERPLLVIPGNHDIPRAPWLRFTSTFDEFLRVWPETEPTH
                     RSDGLVVCGLNSVRAWKQQGGALRRVQVDHVARTFAEAPEGAFRMVALHHHLLGAPWR
                     TAKRTIADRSRVLGELVDAGAELVVSGHVHQSAISERREFEVTPGLVRGTTVVTAPGL
                     GQPRPKRRGEARGLHAFEVYKNALRALTYAWLDGRWALVADRRFPRGREPLALVD"
     gene            3992..4231
                     /locus_tag="VFO88_06840"
     CDS             3992..4231
                     /locus_tag="VFO88_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545276.1"
                     /translation="MADEPSELFDDIYVGLRAGAAGRKKRRGEPLSPEEREALGRWQR
                     MSTGRKAVAVGGFAVGTFGLGFTVGGLVFRRSRKA"
     gene            4257..4703
                     /locus_tag="VFO88_06845"
     CDS             4257..4703
                     /locus_tag="VFO88_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545277.1"
                     /translation="MRPANRKAVRRYLDPGERVRLEARPHGAALVRPLLRALVFAVGG
                     GALVVYAMPHAWPLGVLGALALAYAAFTTLRAVLAWDRTILVVTSAKLLVVYGVVRRR
                     AASAALPAGGAVEVDQGLLGRLLGYGTVVAGDLEVPYVPDPGRLVR"
     gene            4786..>5448
                     /locus_tag="VFO88_06850"
     CDS             4786..>5448
                     /locus_tag="VFO88_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9545278.1"
                     /translation="MRRPTAPQVCVLGVLALALALPATASAQPPAGKLGIATIGGLHT
                     LSAHELGRELDASRAAGARWIRTDVNWASVQSGGRNSWNWAPFDRVIRGARARGLRVL
                     ATIGYTPAWARPAGTPAHHPPRRAKAYARFARAAARRYKRNGVRHWEIWNEPNLAAAW
                     AGGVSALRYGRLLRAGARGIRRANRRAVIITGGLSPAANADGNIAPVTFLSRLYRHGF
                     RRS"
BASE COUNT          741 a         1950 c         1984 g          773 t
ORIGIN      
        1 ggagcagatc gtccgcccga ccgggatcgt cgaccccgag gtcgagctgc gcccgacgaa
       61 gaaccagatc gacgacctcc tgaacgagat ccgccgccgc gaggaggcgg gcgagcgcgt
      121 gctcgacacc accctgacca agaagatggc cgaggacctg accgactacc tgctcgaggc
      181 gggcgtgcgg gcgcgttacc tccactcgga gatcgacacc ctcgagcgga tcgcgatcat
      241 ccgcgagctc cgcctcggcg agtacgacgt cctcgtcggc gtgaacctcc tccgggaggg
      301 cctcgacctg cccgaggtct cgctcgtcgc gatccttgac gccgacaagg aggggttcct
      361 ccgcggccag acggcgctca tccagacgat cggccgcgcc gcgcgcaacg tgaacggacg
      421 cgtgctcatg tacgcggaca agcagacgga ggcgatccgc ggcgcgctcg aggagacgaa
      481 ccgccgccgc gagaagcagc tcgcgtacaa cacggagcac gggatcaccc cggagtcgat
      541 cgtgaagggc gtcagcgaca tcgccgagtt cctctcgctc gagtcgccga acgtccccgg
      601 ccgctcgcgc cggcgcaagg cctcgcgcgt cgaggggatg agcccgtccg aggtcgagaa
      661 gctcgtcgtc tcgctcgagg aggagatgtt cgcggccgcc gaggagctcc gcttcgagta
      721 cgccgcgaag ctccgcgacg agatcaagga gctccgccgc gacctgcagg aagcggccga
      781 ggccgcggcc tagccgctag tcgatcacga agccgacgac cgtctgcgcc accttcccgc
      841 ggtacaggcg gaagatcgtc acgcgccggc cggggaggaa gcggcccttc acgcactcgc
      901 gccggttgca gcgaacgccc gcaacgccgg cgcgcacctc cgcgcgggtc gagccgaagc
      961 cgacgccggt cgccgtccgc tcgttccggc ggttcgtccg cacggcggtc acgttcgtcc
     1021 ggccctggaa gacgacctgg agccgccggt acttgaagat gatgtagcgc ccgaactcgt
     1081 tcgacccgcg ctcgatctcg cgcggctccc cgagcaccgc ccgcacctcc gcccgcgtca
     1141 tcgagagctc gacgcccgcg atcgagcgct gcgggacgat ctccgcggcc gccggcgcgg
     1201 cgagcacgag cgcgaaggcg gcggcgaggg cggcggggag gaagcggcgc acgccccgag
     1261 gctagcgcgt gcttcccgcc gtgactagac cgagatgcga agggccggga tcgctcccgg
     1321 cccttcgccg tcccgtcggc tctcggagag agctagttga gccgcgtgat ccggttccgg
     1381 ggcgtggggg acaggggcga gttggcgccg aggtagtcgg cgaacgcgtc cgtgtccacg
     1441 acgccgccga ggcggttcgt gccctcgcgg agcaccgtga acccgtcccc gccgtcggcg
     1501 aggaagttgt tcacggtgac gcggtaggtc gccagcgggc tgatcggcgt cccgttgatg
     1561 gtgatgctcg acgtggcgat ccggtcgccg atcggggccg acgcgtccca cgtgtagcgg
     1621 aggctgtcgg agatctgcag gatcacctgc cgcgtccccg ggccccactg ctgctcgagc
     1681 acgcgctcga gctggtcgcc cgtcagcgtc atcgtgacga ggttgttgcc gaacggctgc
     1741 accgcgaaga tctcgccgta cgtgacctgg cccggcagct cgccgcccga gatctggttg
     1801 aagacgaggt cggtgcggat cccgcccggg ttcatgagcg cgaccacggc cccgccgagg
     1861 gcgggatcgc tcgtcgccgc gagctgcgcg tcggcgatgt ggtcgccgag cgaggactcg
     1921 ccggccgtgt tgttcgtccg cgtgatgtcc gcggtgatcg agccgacgac gcggttcgcg
     1981 atcggcgcag cgacgacgcg gtagcgctcg atgagctccg tctgagccgg gtcgcgggct
     2041 acgtcgcgcg tgacgatgtt gttgttcacg cggatctcga ccggctgctt cgaggcccgg
     2101 ctgatcgtca tgtcgatgtc ggtcacgatc cggccgaacg aggccgcgct cgagacgggg
     2161 cggccgtcga tcacgcagtt gtacgcctgg tgcgtgtggc cggtgacgaa gaggtcgacc
     2221 tcgtcgctcg tccggttgac gatgtcgacg atcgggcccg agataccggg gcactcgttg
     2281 tacgcgcccg tcggaaaccc gccctcgtgg acgaggacga cgatcgtctt cacgttctgc
     2341 ttgagcagct ccggcacgag cgcgttcacc gtgtccgcct cgtcgtggaa ctcgaggccc
     2401 gcgatgccgg acggggtgac gatctcgggc gtgccctcga gggtcatccc gatgagcgcc
     2461 atcttcacgc cctggaactt cacgatcttg tagggcggga agatcgtgtc gcccgtctcc
     2521 tcccactcga cgttcgcggc gaggaagtcg aactcggcgc cgtcgaagcc ggtgccgtcc
     2581 tggcagccgt ccaccgggtg gcagccgccc tcctgcatgc gcaggagctc ggtcgcgccc
     2641 tcgtcgaact cgtggttccc gacggcgttg aagtcgaggc cgaacaggtt cgccgcctcg
     2701 atcgtcggct cgtcgtggaa gagccccgag agcagcgggc tcgcgccgat caggtcgccg
     2761 gccgacacga cgagcgtgtt cgggttcgtc gcgcgaagct ggtcgatgtg ggacgcgagg
     2821 aactcgacgc cgcctgcgtc gaccgtgccg atgcggccgg acgagcccga cggcggctcg
     2881 aggttgccgt ggaagtcgtt gatcgcgagc agctggacgc tcgtcgtctt cgctttcgcg
     2941 accgcctgcg gcgcggggcc cttcttgccg ggcgccgcca tggccgaggc cgcgaccagc
     3001 ggcagcacca acgcgacgca cgccgcgacc gcagtcctga tgcctttcgt cactgtgtct
     3061 ctcccctcgt aaaggatggt ggcgctcccc ggcgccacac aacgtgcacc cggcatcgta
     3121 gccgcgtttc tcgcgattcg cgctcagtcg acgagcgcga gcggctcgcg gccgcgcggg
     3181 aagcggcggt cggcgacgag cgcccagcgg ccgtcgagcc aggcgtaggt gagcgcccgg
     3241 agcgcgttct tgtacacctc gaaggcgtgg agcccgcgcg cctcgccgcg ccgtttcggg
     3301 cggggctggc cgagccccgg cgcggtcacg accgtcgtcc cgcgcacgag cccgggcgtc
     3361 acctcgaact cgcgccgctc ggagatcgcg ctctggtgga cgtggcccga gacgacgagc
     3421 tccgcgccgg cgtcgacgag ctcgccgagc accctcgagc ggtcggcgat cgtgcgcttc
     3481 gccgtccgcc acggcgcgcc gagcaggtgg tggtggaggg cgaccatgcg gaacgccccc
     3541 tccggcgcct cggcgaacgt ccgggcgacg tggtcgacct ggacgcgacg gagcgctccg
     3601 ccctgctgct tccacgcgcg caccgagttc aggccgcaga cgacgagccc gtccgagcgg
     3661 tgcgtcggct ccgtctccgg ccagacgcgg aggaactcgt cgaacgtgct cgtgaaccgg
     3721 agccagggcg cgcgggggat gtcgtggttc cccgggatca cgagcagcgg gcgctcgagc
     3781 gagcgcagga gccgggccgc gcggtcgtgc tgctcggtgc ggttgcggtg cgtgaggtcg
     3841 ccgctcgcga ccacgagctc ggggtcgaga tccgcgacga gcgtccgcag cgcgtcctcg
     3901 acctcgggct cctcccgcgt gcccgcgtgc aggtcggaga gctggacaac ccgcaggggc
     3961 ggcatcggag ccggagacta tagtgagacg tatggcagac gaaccgagcg agctcttcga
     4021 cgacatctac gtcgggctcc gcgccggtgc cgccgggcgc aagaagcgcc gcggcgagcc
     4081 gctctccccg gaggagcgcg aggcgctcgg gcgctggcag cgcatgtcga ccggacgcaa
     4141 ggcggtcgcc gtgggcggct tcgccgtcgg cacgttcggg ctcgggttca cggtcggcgg
     4201 cctcgtcttc cgccgctcgc gcaaggcctg acgcgtccgg gaacgtagga ggtgcgatgc
     4261 gcccggcgaa ccgcaaggcc gtgcgtcggt atctcgaccc cggcgagcgg gtgcggctcg
     4321 aggcgcgccc gcacggcgcc gcgctcgtgc ggccgcttct ccgcgcgctc gtgttcgcgg
     4381 tcggcggcgg ggcgctcgtc gtctacgcga tgccgcacgc ctggccgctc ggcgtcctcg
     4441 gcgcgctcgc cctcgcgtac gcggcgttca cgacgctccg cgccgtcctc gcgtgggacc
     4501 gcacgatcct cgtcgtcacg agtgcgaagc tcctcgtcgt ctacggcgtc gtgcgccgcc
     4561 gcgccgcctc ggcggccctc ccggccggcg gcgccgtcga ggtcgaccag ggcctcctgg
     4621 gccgcctgct cgggtacggg acggtcgtcg cgggcgacct cgaggtgccc tacgtgcccg
     4681 atccggggcg gctcgtgcgg taacgaacac gggaaggaac accggaaaaa gagtctgtaa
     4741 agaaaccggg tggccttccg ggggctctgc tatcaaatgc ccccgttgcg ccgcccgacc
     4801 gccccccagg tctgcgtcct cggtgtgctc gcgctcgcgt tggcccttcc ggccaccgcc
     4861 tccgcgcagc cgcctgccgg caagctcggg atcgccacga tcggcggcct gcacacgctc
     4921 tccgcccacg agctcggccg ggagctcgac gcctcccgcg ccgccggggc ccgctggatc
     4981 cgcaccgacg tcaactgggc ctccgtccag tcaggcggcc ggaacagctg gaactgggcg
     5041 ccgttcgacc gcgtgatccg cggggcgcgc gcgcggggcc tgcgcgtcct cgcgacgatc
     5101 ggctacacgc cggcgtgggc gcggcccgcg ggaacgccgg cgcaccatcc gccgaggcgt
     5161 gcgaaggcct acgcgcgctt cgcgcgggcg gcggcgcgcc gctacaagcg gaacggcgtc
     5221 cggcactggg agatctggaa cgagccgaat ctcgccgccg catgggcggg cggcgtgagc
     5281 gccctgcgct acggtcggct cctgcgtgcg ggggcccgcg gcatccggcg ggccaaccgg
     5341 cgcgcggtca tcatcacggg cggattgtcc ccggcggcga acgcggacgg caacatcgcg
     5401 cccgtcacgt tcctgagccg tctctaccgg cacgggttca ggcggtcg
//
LOCUS       DASTFC010000231         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1060227,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000231 DASTFC010000000
VERSION     DASTFC010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060227"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFO88_06855"
     CDS             complement(<1..282)
                     /locus_tag="VFO88_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9545279.1"
                     /translation="MRIVIAEDTVLLREGLAGLLEDAGNTVVARVGDAETLLAVVAEH
                     EPELAIVDVRMPPSYEDEGMRAAAEIRRSHPSTAVLVLSQHVETRLAVEL"
     gene            complement(270..>2105)
                     /locus_tag="VFO88_06860"
     CDS             complement(270..>2105)
                     /locus_tag="VFO88_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9545280.1"
                     /translation="VGLLVALRRPRNRIAWILLLGPFAMTVQLPLGLALGEGWALQLD
                     RAMWPLLYAWPIAVAYVFPDGRLLSRRWRWVVGGALVSFLGFVTLSLLDPTPFYGEDA
                     SVRNPLAETAVGEWVERSDLGWLWVVFWLGVLASLVAGAVAVALRLRRSSGIERLQTM
                     WLAWAAALVPLGLLLCGAASLALGDSVFDWILFPFLLVTQAAVAAAVGVAVVRYRLYA
                     IERIVNRTLVYASVTFLLLAVYVGITVAVGVAVGQRSPWAATIATLAVALAFRPLRTR
                     IQDRVDRRFSRARYEGVRRVRTFEDDVRSGRRAPEEIRLVLAETLADPLAELYFWLPE
                     TEAYVDPNGEILADLPSDGRARTDVRRDEGRTAVLLHDPALLERRDLLDSVLDAAALS
                     VEIARLRVEVRLRLAEVEASRTRIVEAGYEERRRLERDLHDGAQQRLVSLGVQLRRLQ
                     LALPRGAEILSPALDTIVAEVGAAIADLRQIAAGVRPARLDEGLAAALRDLARSSPVP
                     VDVVVPSQRVAASVEAAAYFVACEALTNAVKYASPSRVAVRAVRENGELRVSVSDDGI
                     GGAVVRRGSGLAGLRDRVASHGGTLEVVSPHGMGTRIEAVIPCES"
BASE COUNT          327 a          764 c          782 g          232 t
ORIGIN      
        1 gagctcgacg gcgaggcggg tctcgacgtg ctgggagagg acgaggaccg ccgtgctcgg
       61 gtgcgagcgg cggatctcgg ccgcggctct catgccctcg tcctcgtagc tcgggggcat
      121 gcgcacgtcg acgatcgcga gttcgggctc gtgctcggcg acgacggcga gcagcgtctc
      181 ggcgtccccg acccgcgcga ccacggtgtt gcccgcgtcc tcgaggagcc ctgcgagccc
      241 ttcgcgcagg agcacggtgt cctcggcgat cacgattcgc acgggatcac cgcctcgatt
      301 cgggtcccca tgccgtgcgg gctcacgacc tcgagcgttc cgccgtgcga ggcgacccgg
      361 tcccggaggc cggcgaggcc ggagccgcgg cggacgacgg ccccgccgat gccgtcgtcg
      421 gagacgctga cgcgcagctc gccgttctcc cgcaccgccc gcaccgcgac cctcgagggc
      481 gacgcgtact tgaccgcgtt cgtcagcgcc tcgcaggcga cgaagtaggc cgccgcctcg
      541 acgctcgcgg ccacgcgctg ggacggcacg acgacgtcga ccgggaccgg ggacgagcgg
      601 gcgaggtcgc gaagcgccgc cgcgaggccc tcgtcgagcc gggccgggcg aacgcctgcc
      661 gcgatctggc gcaggtcggc gatcgccgcg ccgacctccg cgacgatcgt gtccagggcc
      721 ggagagagga tctcggcgcc gcggggcagg gcgagttgca ggcgcctgag ctgtacgccg
      781 agcgacacga ggcgttgctg agcaccgtcg tgaagatcgc gctcgaggcg ccgccgttcc
      841 tcgtagccgg cctcgacgat cctcgtcctc gacgcctcga cctcggcgag ccggaggcgg
      901 acctccacgc ggagccgtgc gatctcgacc gacagcgcgg cggcgtcgag cacgctgtcg
      961 agcagatcgc gccgctcgag aagggccggg tcgtgcagga ggactgccgt ccgtccctcg
     1021 tcgcgccgga cgtcggtgcg cgccctcccg tcgctcggca ggtcggcgag gatctcgccg
     1081 ttcggatcca cgtaggcctc ggtctccggc agccagaagt acagctctgc gagcgggtcg
     1141 gcgagtgtct cggcgaggac gaggcggatc tcttcgggcg cgcggcggcc gctgcgcacg
     1201 tcgtcctcga acgtccggac gcggcgcacc ccctcgtagc gggcccggct gaagcggcgg
     1261 tcgacccggt cctggatgcg cgtccggagc ggccggaagg cgagcgcgac ggcgagggtc
     1321 gcgatcgtcg ccgcccacgg cgaccgctgt ccgacggcga cgccgacggc aaccgtgatg
     1381 ccgacgtaca cggcgaggag caggaacgtg acggacgcgt agacgagggt gcggttgacg
     1441 atccgctcga tcgcgtagag gcgatagcga acgacggcga ccccgaccgc cgccgcgacc
     1501 gccgcctgcg tcacgagcag gaacggaaag aggatccagt cgaagaccga gtcgccgagc
     1561 gcgagcgagg ccgcgccgca caggaggagc ccgagcggca cgagcgccgc cgcccaggcg
     1621 agccacatcg tctggagccg ctcgatcccg ctggaccggc gaagccgcag cgcgactgcc
     1681 accgcgcccg cgacgaggct cgccagcacc ccgagccaga agacaaccca gagccacccg
     1741 aggtcggagc gttccaccca ctcgcccacg gccgtctccg caagcgggtt tcgaacggac
     1801 gcatcctcgc cgtagaaggg ggtggggtcg agcagcgaga gcgtcacgaa gccgaggaag
     1861 ctgacgagcg cgccgcccac gacccagcgc cagcggcgcg acagcaagcg cccgtcgggg
     1921 aacacgtacg cgacggcgat cggccaggcg tagagcagcg gccacatcgc ccggtccagc
     1981 tgcagcgccc agccttcgcc gagcgcgagc ccgagcggga gctggaccgt catcgcaaac
     2041 ggcccgagca gcaggatcca ggcgatccga ttgcggggcc ggcgaagcgc gacgagcagc
     2101 ccgac
//
LOCUS       DASTFC010000232         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_351258,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000232 DASTFC010000000
VERSION     DASTFC010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351258"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFO88_06865"
     CDS             complement(<1..327)
                     /locus_tag="VFO88_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545281.1"
                     /translation="MCGIAGYSLHPDSGIDRTLAAQSLLAAIAERGSDAVGYAYRSGD
                     GPIVVHKQRTGASQLLEAVSIPRDATQGLFHVRDYTKGHPSLAANNHPIRHGAVVGIH
                     NGVIRND"
     gene            479..1495
                     /locus_tag="VFO88_06870"
     CDS             479..1495
                     /locus_tag="VFO88_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545282.1"
                     /translation="MEHPLSNLPLPDPADETPRLELAPHPDPEVEDAAAEDEDDEDDE
                     PEDEADAEAKPKPKAKREPAQTQDPLKLYVRQIGDGPLLTPAEERELARLKDLGDEDA
                     KRRLIEANLRLVMSITRNYVNAGVPLLDLIQEGNLGLIRAVEKFDYKLGYKLSTYATW
                     WIRQAVTRAIADQGRTIRLPVHVVEQVRKMTRARRVLTQKLNRDPLPEEIAAESGFDL
                     KRVRELLDLVEDPVSLETPVGDGESIYADMLEDVNAEQPDTVLAELLRTKELQAALIA
                     LNERMRHVLALRFGLNGEKPKTLEEVGTELGVTRERVRQLESRALRELQAAAPELKLY
                     LRAE"
     gene            complement(1522..2061)
                     /locus_tag="VFO88_06875"
     CDS             complement(1522..2061)
                     /locus_tag="VFO88_06875"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9545283.1"
                     /translation="MPVQEAKLVRTENGLAPEGDGWFVVNAREAVWEENAVMGGGTGF
                     EAAANRFPDFGINIVVLDPGQPNCMYHGENEQEDFLVLGGECLLLVEGEERTLGQWDF
                     VHCPAWTEHVFVGAGDGPCAILMVGGRRNKGVLYPVVDVALKHKAGVETETRDAKEAY
                     APFPGWTDRPYRQGDLPGD"
     gene            2096..2821
                     /locus_tag="VFO88_06880"
     CDS             2096..2821
                     /locus_tag="VFO88_06880"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HET9545284.1"
                     /translation="MFWDLHTHMVPSGDDGVRTEEDGLALCREAAERGTSVLFATPHV
                     WPHFELSEAREEAVRAAHARMAPRAAEFGLDLRLGFELTPAPALLEADLRRFRLGGLP
                     AVLMELPFDGPLGLAVRLAEEIEAAGLTPVIAHPERADEVIDDPGQARAFAARGWLLQ
                     VNATSLLGRHGRGPRRTGWRLVEEGLASVMGSDGHRASRPPFLDEAYEAVRARVGEEA
                     AGDLFGAVALQRLESLAPAEPPS"
     gene            complement(2846..3142)
                     /locus_tag="VFO88_06885"
     CDS             complement(2846..3142)
                     /locus_tag="VFO88_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545285.1"
                     /translation="MPAAAPRIDSRLLAALARIDDGSLPIAEITRRLGSVADWLEIPR
                     PSYEHVRRLVHAQRRRGAYPTAGDILLQVAANERPPVAIMEHLLGLGPTARPAP"
BASE COUNT          471 a         1148 c         1168 g          442 t
ORIGIN      
        1 gtcgtttcgg atcacgccgt tgtggatccc gacgaccgcg ccgtgcctga tcggatggtt
       61 gttcgcggcg agcgacggat ggcccttcgt gtagtcgcgg acgtggaaga gcccctgcgt
      121 cgcgtcacgg gggatcgaga cggcctcgag gagctggctc gcgcccgtgc gctgcttgtg
      181 gacgacgatc gggccgtcgc ccgagcggta cgcgtacccg accgcgtccg agccccgttc
      241 ggcgatcgcc gccagcagcg actgcgcggc cagcgtcctg tcgatcccgc tgtccggatg
      301 caagctgtat ccggcgatgc cacacacgag tgaatgactc tacttaaacc ttccgacgac
      361 agggccgcaa caggtgaggt gcgtcttaaa ggttcgttat ggctttcgta ctgcgcagcg
      421 aaacctaaat cccccgccgg ggggtagcac cgctcgaagc cccgccgata cagggggcgt
      481 ggagcacccc ctctcgaacc tgccgcttcc cgacccggcc gacgagacgc cccgcctcga
      541 gctcgccccg caccccgacc cggaggtcga ggacgcggcc gccgaggacg aggacgacga
      601 ggacgacgag ccggaggacg aagccgacgc cgaggcgaag ccgaagccca aggcgaagcg
      661 cgagcccgcg cagacgcagg accccctgaa gctctacgtc cgccagatcg gcgacggccc
      721 gctcctcacg cctgccgagg agcgcgagct cgcgcgcctc aaggacctgg gcgacgagga
      781 cgcgaagcgg cggctgatcg aggcgaacct ccgcctggtc atgtcgatca cgcgcaacta
      841 cgtgaacgcc ggcgtgccgc tcctcgacct catccaggag ggcaacctcg gcctgatccg
      901 cgcggtcgag aagttcgact acaagctcgg gtacaagctc tcgacgtacg cgacgtggtg
      961 gatccgccag gcggtcacgc gcgcgatcgc cgaccagggc cggacgatcc gcctgcccgt
     1021 ccacgtggtc gagcaggtgc gcaagatgac ccgcgcccgc cgagtcctca cgcagaagct
     1081 gaaccgcgac ccgctccccg aggagatcgc ggccgagagc ggcttcgacc tgaagcgcgt
     1141 ccgagagctc ctcgacctcg tcgaggaccc ggtctcgctc gagaccccgg tgggcgacgg
     1201 ggagtcgatc tacgcggaca tgctcgagga cgtgaacgcc gagcagccgg acacggtgct
     1261 cgccgagctc ctgcgcacga aggagctcca ggcggcgctc atcgcgttga acgagcggat
     1321 gcgccacgtg ctcgccctcc gtttcggcct gaacggcgag aagccgaaga cgctcgagga
     1381 ggtcggcacc gagctcggcg tgacgcgcga gcgggtccgc cagctcgagt cgcgcgccct
     1441 ccgcgagctg caggccgccg cgcccgagct caagctgtac ctccgcgccg agtagccgcc
     1501 ggcgggcccg gcgccgcgcc gtcagtcgcc cggaagatca ccctggcggt acgggcgatc
     1561 cgtccagccg gggaacgggg cgtaggcctc cttcgcgtcg cgcgtctcgg tctcgacccc
     1621 ggccttgtgc ttcagggcga cgtcgacgac ggggtagagg acgcccttgt tgcgccggcc
     1681 gccgaccatg aggatcgcgc acgggccgtc gcccgcgccg acgaagacgt gctccgtcca
     1741 ggcggggcag tggacgaagt cccactgccc gagcgtgcgc tcctcgccct cgacgaggag
     1801 caggcactcg cccccgagga cgaggaagtc ctcctgctcg ttctcgccgt ggtacatgca
     1861 gttcggctgg ccggggtcga gcacgacgat gttgatcccg aagtcgggga agcggttggc
     1921 ggccgcctcg aagccggtcc cgccacccat cacggcgttc tcctcccaga ccgcctcgcg
     1981 ggcgttgacg acgaaccagc cgtcgccctc cggcgcgaga ccgttctccg tgcggacgag
     2041 cttcgcttcc tgtaccggca tctcgggctc ctctcggtgg ctaccgttgg gatcggtgtt
     2101 ctgggatctg cacacgcaca tggtcccctc cggggacgac ggcgtgcgga ccgaggagga
     2161 cgggctcgcg ctctgccgcg aggcggccga gcgcgggacg agcgtcctct tcgcgacgcc
     2221 gcacgtctgg ccgcacttcg agctctccga ggcgcgcgag gaggcggtgc gcgccgccca
     2281 cgcccggatg gcgccccggg cggcggagtt cgggctcgac ctgcggctcg ggttcgagct
     2341 gacgcccgcg ccggccctgc tcgaggccga cctgcgccgc ttccgcctcg gcgggctgcc
     2401 cgccgtgctc atggagctgc cgttcgacgg cccgctcggg ctcgcggtgc gcctggccga
     2461 ggagatcgag gcggccgggc tcacgccggt gatcgcgcat ccggagcggg cggacgaggt
     2521 gatcgacgac ccgggacagg cgcgcgcgtt cgcggcgcgc ggctggctgc tccaggtcaa
     2581 cgcgacgagc ctcctcggcc gccacggccg cggcccgcgc cggacgggct ggcggctcgt
     2641 cgaggagggg ctcgcctccg tgatgggatc ggacggccac cgcgcgtcgc gcccgccgtt
     2701 cctcgacgag gcgtacgagg ccgtgcgcgc gagggtgggg gaggaggccg cgggcgacct
     2761 cttcggcgcg gtcgccctcc agcggctgga gtccctggcg cccgcggagc ccccgagcta
     2821 gtaacacagt gttactagct ccgggtcacg gggcgggtcg ggctgtcggt ccgaggccga
     2881 ggagatgctc catgatcgct acgggcgggc gctcgttcgc ggcgacctgg agcaggatgt
     2941 cgccggccgt cggataggcg ccgcgcctgc gctgggcgtg gacgagccgc ctgacgtgct
     3001 cgtagctcgg gcgcgggatc tcgagccaat cggcgacgga gccgaggcgt cgggttatct
     3061 cggcgatcgg cagcgagccg tcgtcgatcc tcgcgagcgc cgcgaggagg cgcgagtcga
     3121 tccgcggtgc ggcggcaggc atctccgaac gatcgccgcc cgggagcaca ggccgtgtgc
     3181 cgattcggtg ccgaacgatc cccaagtaac actgtgttac aaggaagcg
//
LOCUS       DASTFC010000233         4267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_466785,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000233 DASTFC010000000
VERSION     DASTFC010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466785"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFO88_06890"
     CDS             <1..304
                     /locus_tag="VFO88_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545286.1"
                     /translation="TGQSDGAFMAISSPTNQVNSTVVIPKCPGSPCTDTVPTEQVTGA
                     DGRPYRVDTYITWKAITSQAGVAGRNVKLVTIVVRDAATNRIYARVSSSFDQLTGI"
     gene            complement(634..1425)
                     /locus_tag="VFO88_06895"
     CDS             complement(634..1425)
                     /locus_tag="VFO88_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545287.1"
                     /translation="MAKRNKAADAMKKQKEARQKKVLIGLVPLLLLLMVWQGPGMLSA
                     FTGGSAEPPPVLPPEGSTGTTGTGDGAGTALDPATGAAPTTAAPGTSAAVDPAVPASL
                     PETGETVAPGPGQLVSFDRFLGKDPFRPQVVPTETTGGGGGTGAGDGDGDDGGAKGGG
                     GASPPGSGGGGTPPPAPPPPSTYEAAVIEVNGQQQTVAKRGTFPSFDPVFRLLAIRRA
                     SIEFGLVSGEFTAGQNRIVVRAGESVTLVSEPDGQRFVVRLVAVG"
     gene            complement(1425..2162)
                     /gene="pilO"
                     /locus_tag="VFO88_06900"
     CDS             complement(1425..2162)
                     /gene="pilO"
                     /locus_tag="VFO88_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET9545288.1"
                     /translation="MKKQIPLTPVLAAGLLIVVLVGWFALVGPKRAQSASLDEEIADY
                     ETKIMLATEAKKASDAPPEVEIRVADLFRLAKAMPDREDMPGVILELNSVALSTGIAF
                     VSIAPGTAVTREGHYSVPITLTFEGNYYDLTDFLFRLRNLVSVRDGVLDATGRLYTLD
                     SLEFNEAEAGFPEIQATLVLSAYAFGVPVVPGVTPPPPAETGTTSAGQTTTGQTTTGE
                     TTTTDESDGPPADPTGDGNPEAAGGTP"
     gene            complement(2159..3067)
                     /locus_tag="VFO88_06905"
     CDS             complement(2159..3067)
                     /locus_tag="VFO88_06905"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET9545289.1"
                     /translation="MDLKQEIKLSDLFRRKPKDPAEPEIAAAADEKPKKDRSFFTLRR
                     KKDDAGEAAPPEAKPPKEPRRRKEKKAKSPAQTLPDVPLMRAFNLLPREEGRRETMTT
                     EDGGERRPSTVQLVLAVVGLLLVAALASIFLITNATVADKQTELDGLRSELAALDAAA
                     EKPSAPEPDGTLVQEQNNRTGALATALAQRVAWDRLLRELSLVLPDDVWLTRLTAKAG
                     GTGPVDPNAPPAAAGAAPPASTFEIGGYTYKQENVAQLLSRMGVLPQVASVQLLGSTA
                     TQIGEEDVVEFTISATMKPNGAGGTA"
     gene            complement(3067..4131)
                     /gene="pilM"
                     /locus_tag="VFO88_06910"
     CDS             complement(3067..4131)
                     /gene="pilM"
                     /locus_tag="VFO88_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET9545290.1"
                     /translation="MGKEVVGLKIGASALAAARVRVNGSAQVQQVAREPLPSGIVVGG
                     EVRDPDALAAALKDFFKKHKLPRRAVRIGISNNRIGVRTIELAGIEDARQLANAVRFR
                     AQEALPIPLDEAVLDYQVLSEGRSAEGQAVRKVLLVVAYRDLVNGYAHACRAAGLKLT
                     GVDLEAFALLRALSPGPPEPGAGRSALVAVAIGSERSTLAVSDGSTCEFTRVLDWGGS
                     ALTIALARSLGVELEEAERIKGLLSLDETPGAVQGLTAEQTTQAVEVLRNGLQSFGRE
                     LVSSLQFYQSQPDSLGIREVVLAGGTAQLGGLAKAIQGLIGVTVRVGDPLVAVEAGKK
                     ARDASNPALAVPIGLGMAAD"
BASE COUNT          540 a         1542 c         1580 g          605 t
ORIGIN      
        1 caccggccag agcgacggcg cgttcatggc gatctcctcg ccgacgaacc aggtgaactc
       61 gaccgtcgtc atcccgaagt gcccgggctc cccgtgcacg gacaccgtgc cgacggagca
      121 ggtcaccggc gcggacgggc gtccgtaccg ggtggacacg tacatcacgt ggaaggcgat
      181 cacgagccag gccggcgtcg cgggccgcaa cgtgaagctc gtgacgatcg tggtgcgcga
      241 cgccgccacg aaccggatct acgcgcgcgt ctcgtcctcc ttcgaccagc tgaccgggat
      301 ctagcgccag ggcgcgcctc gcgcgcgcgt agcgggtcgg cgcccgtaag ggggtcagac
      361 cccgcaccaa ggcgcaggag aatgtgtgcc gacttcgcaa cgtcgctgct acgagcggcc
      421 ttctcgacgg cgcgaacctg tcacggaagc gtgacgcgcc cgcgttcacg gggtcagacc
      481 ccgtgtcgga gagcgcacgc cttcgtgccg gcacgatgac gtccctgcaa acgagccgct
      541 tttccgcagc ggaaggtgtg ccgaacgtcg tgcgccgtcc gtgcttacgg ggtcagaccc
      601 cgtaagggaa cacgcgccgc ggccgcgggg tcgctagccg accgcgacga gtcgcacgac
      661 gaagcgctgc ccgtcgggct cgctgacgag ggtgacggac tcccccgcgc ggacgacgat
      721 ccggttctgg ccggcggtga actcgcccga gacgaggccg aactcgatgc tcgcccgccg
      781 gatcgcgagc aggcggaaga ccgggtcgaa cgaggggaac gtcccccgct tggcgaccgt
      841 ctgctgctgg ccgttcacct cgatcaccgc ggcctcgtac gtgctcgggg gcggaggtgc
      901 cggaggaggc gtaccgccgc cgccgctgcc gggcggagaa gcgccgccgc cgcccttcgc
      961 gccgccgtcg tcgccgtcgc cgtcaccagc accggtacca ccgccgccgc cggtcgtctc
     1021 cgtggggacg acttgcgggc ggaacggatc cttgccgagg aagcggtcga acgagacgag
     1081 ctgccccggg ccgggcgcca cggtttcccc ggtctcgggc agcgacgcgg gcaccgcggg
     1141 gtcgaccgcc gccgacgttc cgggcgcagc cgtcgtcggc gcggcgccgg tcgcggggtc
     1201 gagcgcggtt ccggcgccgt cgccggtacc ggtcgtgccg gtcgagccct ccggcgggag
     1261 cacgggcggc ggctcggcgc tcccgccggt gaacgcgctc agcatgccgg ggccctgcca
     1321 gaccatcagg agcaggagga gcggcacgag cccgatgagg accttcttct gccgcgcctc
     1381 cttctgcttc ttcatcgcgt cggcggcctt gttcctcttc gccattacgg ggtgcctccc
     1441 gcggcctcgg ggttgccgtc gcccgtcggg tcggcgggcg ggccgtccga ctcgtcggtc
     1501 gtcgtcgtct cgccggtcgt cgtctgcccg gtcgtcgtct ggccggccga cgtcgtgccc
     1561 gtctccgcgg gcggcggcgg ggtgacgccg gggacgaccg gcacgccgaa cgcgtacgcg
     1621 gagaggacga gcgtcgcctg gatctcgggg aacccggcct cggcctcgtt gaactcgagc
     1681 gagtcgagcg tgtagagccg cccggtcgcg tcgaggacgc cgtcgcggac gctcacgagg
     1741 ttccggaggc ggaagaggaa gtccgtcagg tcgtagtagt tgccctcgaa cgtgagcgtg
     1801 atcggcaccg agtagtgccc ctcgcgcgtc accgccgtgc cgggcgcgat cgacacgaac
     1861 gcgatgcccg tcgagagcgc gaccgagttc agctcgagga tgacgccggg catgtcctcg
     1921 cggtccggca tcgccttcgc gaggcggaag aggtcggcga cgcggatctc gacctcgggc
     1981 ggggcgtcgg aggccttctt cgcctccgtc gcgagcatga tcttcgtctc gtagtcggcg
     2041 atctcctcgt cgagcgaggc gctctgggcg cgcttcgggc cgaccagcgc gaaccagccg
     2101 acgagcacga cgatcaggag cccggcggcg agcacgggag tcagcgggat ctgcttcttc
     2161 acgccgtccc ccccgccccg ttcggcttca tcgtcgccga gatcgtgaac tcgaccacgt
     2221 cctcctcgcc gatctgcgtc gcggtcgagc cgagcagctg gacggaggcg acctgcggga
     2281 ggacgcccat ccggctgagg agctgcgcga cgttctcctg cttgtacgtg tagccgccga
     2341 tctcgaacgt gctcgccggg ggggcggcgc ccgcggcggc gggcggggcg ttcgggtcga
     2401 cggggcccgt gccgccggcc ttcgcggtga gccgcgtgag ccagacgtcg tcgggcagga
     2461 cgagcgagag ctcccgcagg agccgatccc acgcgacccg ctgcgcgagc gcggtcgcga
     2521 gcgcgccggt gcggttgttc tgctcctgga cgagcgttcc gtccggctcg ggcgcgctcg
     2581 gcttctcggc cgcggcgtcg agcgccgcaa gctcgctgcg caggccgtcg agctccgtct
     2641 gcttgtccgc gacggtcgcg ttcgtgatga ggaagatcga ggcgagcgcg gccacgagga
     2701 gcagcccgac gacggcgagg acgagctgga cggtgctcgg ccggcgctcg ccgccgtcct
     2761 ccgtcgtcat cgtctcgcgg cggccctcct cgcgggggag gaggttgaac gcccgcatga
     2821 gcgggacgtc gggcagcgtc tgcgccgggc tcttggcctt cttctccttg cgccggcggg
     2881 gctccttcgg aggcttcgcc tcggggggcg ccgcctcgcc ggcgtcgtcc ttcttccgcc
     2941 ggagggtgaa gaacgagcgg tccttcttcg gcttctcgtc ggccgcggcc gcgatctcgg
     3001 gctcggccgg gtccttcggc ttgcgccgga agaggtccga gagcttgatc tcctgcttca
     3061 ggtccatcag tcggccgcca tccccagccc gatcggcacg gcgagcgcgg ggttcgacgc
     3121 gtcgcgcgcc ttcttgccgg cctcgaccgc gacgagcggg tcgccgacgc gcacggtgac
     3181 gccgatcagc ccctggatcg ccttcgcgag cccaccgagc tgcgccgtgc cgccggcgag
     3241 cacgacctcg cggatcccga gggagtccgg ctgcgactgg tagaactgga gcgaggagac
     3301 gagctcgcgg ccgaagctct gcaggccgtt ccggagcacc tcgaccgcct gcgtggtctg
     3361 ctcggccgtc aggccctgca ccgcgccggg cgtctcgtcg agcgagagca gccccttgat
     3421 gcgctcggcc tcctcgagct cgacgccgag cgagcgggcg agcgcgatcg tgagcgccga
     3481 gccgccccag tcgagcacgc gggtgaactc gcacgtggag ccgtccgaga cggcgagcgt
     3541 cgagcgctcc gagccgatcg cgacggcgac gagcgcgctc cgcccggccc ccggctccgg
     3601 cggcccgggc gagagcgcgc ggagcagcgc gaacgcctcg aggtcgacgc ccgtgagctt
     3661 gagcccggcc gcgcggcacg cgtgcgcgta gccgttcacg aggtcgcggt aggcgacgac
     3721 gagcagcacc ttccgcaccg cctggccctc cgcgctgcgt ccctcggaga ggacctggta
     3781 gtcgaggacg gcctcgtcga gcgggatcgg gagcgcctcc tgggcgcgga agcggaccgc
     3841 gttcgcgagc tggcgcgcat cctcgatccc ggcgagctcg atcgtgcgga cgccgatccg
     3901 gttgttcgag atgccgatcc ggaccgcgcg gcgcgggagc ttgtgcttct tgaagaagtc
     3961 cttgagggcg gccgcgagcg cgtccgggtc gcgcacctct ccgccgacga cgatgccgga
     4021 cggcagcggc tcgcgcgcga cctgctgcac ctgcgcggag ccgttcacgc gaacgcgcgc
     4081 ggccgcgaga gccgaggctc cgatcttcag tccgacgacc tccttgccca cggcccgcgg
     4141 ctcccggcct agcttccggc cgctcgcagg ccgcccagca gggcgtcgcc gcccgcggcc
     4201 atggtggtga ggcccccgcc gcgctcgagg agcccgacga gctgctcggg ccggctcgag
     4261 cggttga
//
LOCUS       DASTFC010000234        12533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_911896,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000234 DASTFC010000000
VERSION     DASTFC010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12533
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911896"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..990)
                     /locus_tag="VFO88_06915"
     CDS             complement(91..990)
                     /locus_tag="VFO88_06915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9545291.1"
                     /translation="MTFRPATPADAEAIAELVRAYDRAHGGETGTDADEVRDDWTWQG
                     FELERDTLVVEDDGRVVAYAQEVRRPPEGEIQVDACVHPEARDRGRADELYRRMEERA
                     LELGAARLVTGVFATDLWCAELLAARGWASTRSLLRMATDLEAPPSPPAWPEGIETRT
                     FRPGDEAAFHAVAVEAFADDPTYEAGTLERWSADRLDRSAFDPELWTLALAAGRPAGV
                     AVGYAAGPGGYLELLGVVPAWRRRGLGRALLVQSFAAFHRQDRTHVSLHVDADNTTGA
                     RRLYHDAGMRETHRIDRWERPAG"
     gene            complement(987..1655)
                     /locus_tag="VFO88_06920"
     CDS             complement(987..1655)
                     /locus_tag="VFO88_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9545292.1"
                     /translation="MGAGDRYATFDCYGTLIDWNGGIGGELGRLWPDADREALLERYH
                     EIEPRVQLGGPIPYRDVLAESLRLLAESEGLELPPGEEGALGESLPRWPAFPEVPGEL
                     AELRARGWKLVILTNSDPDFIAASIERIGVEFEDAITADRAGSYKPAHGHFERFRELH
                     APEPGRHVHVAASAFHDLGPAAELGIPAVWINRLGEASEHPRAAELTTLAGLADTLDG
                     LVPP"
     gene            complement(1732..2988)
                     /gene="fabF"
                     /locus_tag="VFO88_06925"
     CDS             complement(1732..2988)
                     /gene="fabF"
                     /locus_tag="VFO88_06925"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9545293.1"
                     /translation="MGTIDNGRRVVVTGLGAVTPLGNDPETYWKNLTAGESGAGPITM
                     FDTSEFPVKFACELKGFDPTEYIERRKARKMDRFAQMILAAARQAEADSGLDVAAETD
                     RIGVSVATGIGGLQSFQDCAYTLKDRGADRVNPQSIPAIIPNMGAAWVSMELGTRGPL
                     TSQCTACAASNMAVGEGMDAIRLGRADVVLAGGTEAGITEVGIAGFGAMRALSRRNDA
                     PERASRPFDAGRDGFVMGEAGAIVVLESLEHAEARGAKVYAEIVGYGLSSDAQHVTEP
                     DPTGRHPARAIQMALDSAGVSREEIDYVNAHGTSTPLGDASETRVIKLALGEEKAYAT
                     PISSTKGATGHCLGASGAIEAIACILAVRDGIVPPTINYEDPDPECDLDYIPNESRET
                     DVRYALSNNFGFGGHNACLVIKRWDE"
     gene            complement(2999..3811)
                     /gene="fabG"
                     /locus_tag="VFO88_06930"
     CDS             complement(2999..3811)
                     /gene="fabG"
                     /locus_tag="VFO88_06930"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET9545294.1"
                     /translation="MIDRCGRSPSATQTASRSSRRRSPLSSFCRLDERVAIVTGGSRG
                     IGAAVCRELARAGATVAVNYRSNADLASGIADEIGGIAVEGDVGDPESAKAIVERVES
                     ELGDVDILVNNAGVTRDTLIARMSDEEWREVMSTNLDGAFHMSRAVARKMLKRRSGAI
                     VNMTSFVGLHGNPGQTNYAATKAGIIGFTKALAKELGGRGVRVNAVAPGYIETELTTV
                     LSDEIREFLLTATPMGRLGSPDDVAGAVRFLCSDEAGYVTGAVLLVDGGLGM"
     gene            complement(3732..4658)
                     /gene="fabD"
                     /locus_tag="VFO88_06935"
     CDS             complement(3732..4658)
                     /gene="fabD"
                     /locus_tag="VFO88_06935"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9545295.1"
                     /translation="MSRLAFLFPGQGSHELGMGRAFAEAYPEARAVYDDAAEAVGFDL
                     ARLCFEGPLEELTKTELQQPALVATSLACLRAVETTGVRPDVVLGHSVGEYAALAASG
                     ALSDHDAVALVSARGEVTAAAAEESPGAMAAVIGMEDADVEALCGEIGGVWPANYNCP
                     GQLVVSGETASVDRLVEEAPGRGARKVVKLRVTGAFHSPLVASAADGLRPAVERAGWR
                     EPEIPFMSTVTAALEPASALPQILLDQLTAPVRFTHAVAALVADGVETFVEVGPGQVL
                     SGLVRRCDRSVRTLSVGDPDGLAKLEEAVSAV"
     gene            complement(4655..5671)
                     /locus_tag="VFO88_06940"
     CDS             complement(4655..5671)
                     /locus_tag="VFO88_06940"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9545296.1"
                     /translation="MIAAVEGKGRRVGITGVGVQVPEKVLTNADLERMVDTSDEWIVE
                     RTGIRERRIAADDEAASDLAIPASRQALERAGVDPADLDLVLVATSTPDMLFPSTGAI
                     VADALGAKKAAGYDTLAACSGFLYGLSQAFGLVSGGVAENALVVGTETLSKIVNWNDR
                     GTCILFGDGAGAAVIEPVADGGFIGFELGVDATYGLDLCLPGGGSRHPTSAETVQNEL
                     HWVQMNGREVFRVATRLMVSSANDLLAAVGATIDDVDLYVAHQANQRIIDHAARNLGL
                     PQEKVFLNIDRYGNTSAASIPLGLADALDEGRLRPGMRVLMSAVGGGITWGSAYMTWS
                     GERA"
     gene            complement(5668..6111)
                     /gene="fabZ"
                     /locus_tag="VFO88_06945"
     CDS             complement(5668..6111)
                     /gene="fabZ"
                     /locus_tag="VFO88_06945"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET9545297.1"
                     /translation="MSGPLDRAAIEAILPHREPFLLLDEVTELEPGARVVARKLVRED
                     EWYLPGHFPGNPIMPGVLMVEAMAQCGAVAVLSEKANRGKLALFAGIDDVRFKRLVRP
                     GDELELVCELERLRGPVGRGKARASVDGELAVRGTLLFALTDMPE"
     gene            complement(6108..6719)
                     /locus_tag="VFO88_06950"
     CDS             complement(6108..6719)
                     /locus_tag="VFO88_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9545298.1"
                     /translation="MPVPVIQAYRKLGDLALVAKARAGDRPALDVLVERYSPRVNSLA
                     AQLMGDLEDARDAAQESLVKLCTKLRQFRGDAQFATWMHRLVVNTCRDAQERKRVRAT
                     EPLTEEEPAGEDGDPLRATLLGDLRADLVHGLARLSADQRSVVVLRDVLGFSYEDIAR
                     AASMPVGTAKCYVHRGRQRLRDGLEESLGSTVAPGSASAGRSG"
     gene            6831..7550
                     /locus_tag="VFO88_06955"
     CDS             6831..7550
                     /locus_tag="VFO88_06955"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET9545299.1"
                     /translation="MEPLLEGAFGRPYRYEPTVASTQGLLAPDDPEGAVAACEEQTAG
                     RGRLGRTWEAPYGTAILVSILLRPPAGRRVQELSLVAGIATALTAERALGLASQVKWP
                     NDVLVNRSKVAGVLAEARDDAVVLGIGLNVNQRRDELPSGGTPPAASLRTIDAVVRER
                     APLLALLLAEVEKAYARWREGGLAELYVDLGPRDFLRNRRVRVGDEAGLAIAIDREGR
                     LEVDVGGERRLVESGEVTYLR"
     gene            complement(7547..9703)
                     /locus_tag="VFO88_06960"
     CDS             complement(7547..9703)
                     /locus_tag="VFO88_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9545300.1"
                     /translation="MALACAGPASAGVSVASRDVPLAGSGAERAAAGAIRVLPAQTAP
                     ARFHLVGLHWQGPGDAEFRTARIGGAWSAWRPARPETEDGPNAGDREARAKAGWELGN
                     PWWTGAARRLQVRVSGRVTRLRAHYVTSPVREAPRAAEPAARTLAAAPPQPALVKRAA
                     WGANESIVRGTPSYASRLSFAVVHHTAGSKPTSAAQSAAIVRGIQSYHVLSNGWNDIA
                     YNFLVDPFGQIFVGRKGGAAKNVIGAHAQGFNTGSTGIAVLGTYEGGTISAKARTALT
                     ALLAWRLDVAHVDPASRLTWTSGGNPKFPAGTPVSLNAVSGHRDTGSTSCPGARLHGE
                     LGSLAAAARALGGPKIFSPRSTGALGGPVRFTATLSEARGWTVDVLDAVGNRVAGGSG
                     TTKAVDWTWGSAGAPPGRYTYAIAAGEGVRAATGVVGGSEPPLELGALAPKPVVTTPN
                     GDGVGDRQRIHFTLTRSATVEARLETSEGEPVTTLVANRALGAGARLVGWKGKDANGV
                     KVPDGRYVLVVEATSGPETVTRTAAVRVDRTLAHLSATPTPFSPNGDGTLDTLTVGFE
                     LKRAASVDARVMAGSRRVATLRDGSLSLGKKSFVWDGREPGGSPAADRTYTVIVDATT
                     ASLGTRRLTAKVVVDTTKPVTRILRARRVQGGTSLRLSVSEDVRLAIRLGGRTFNRSL
                     ARGTWTFRLPVRGTSVTVSSFDLAGNAGKPVSRRVG"
     gene            9827..11170
                     /locus_tag="VFO88_06965"
     CDS             9827..11170
                     /locus_tag="VFO88_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET9545301.1"
                     /translation="MRFLRLLSVAALLTGTALALAGAALSQEDGDRPRVLAVEFANDV
                     NPVTQDYLTDAIDRANDEEFDAVVILMDTPGGLDSSMREIIQAELDSEVPVVVYVYPP
                     GSRAASAGVFLAMAADVAAMAPQTNIGSSTPIATSGEEIPEDLRRKVVNDAAVFVREL
                     AEEHGRNGEWAESAVRRASNIGAREALEENVVDFVSPDLETLLEDMDGFETKPKGIVM
                     NTAGAEVVNVEMGLWKQILDLLVDPNLIVLLMSIGILGITIEILNPGLIFPGTVGAIS
                     LIMGLFGLQVLPISWAGLLLLLLSFVFFAAEAFVASHGALALAGAVSFVFGSLLLFDP
                     AGEAYAVSLWVSLAIAGTLALVLGVALGKAVQLRRTKPQTGQDEMLGAVGIVRHALDP
                     DGYVFVHGELWRARVDNGPVEPGTRVQVDVVRDDLVLEVSPVETPVPAAPAGGTA"
     gene            11180..11953
                     /locus_tag="VFO88_06970"
     CDS             11180..11953
                     /locus_tag="VFO88_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET9545302.1"
                     /translation="MSPALIAVAAVLVVLIFLLSASIRVAREYERGVIFRLGRLKSPP
                     KGPGLFLLIPVIDKMVRVDLRTVTLNVPPQEVITKDNVPVRVNAVAYFRIIDPERAIV
                     QVENYMVATSQISQTTLRSVLGQHMLDELLSERDKINEILQRIIDEATSPWGVKVSIV
                     EVKDVEIPAGMQRAMARQAEAERERRAKVIAAEGEFQASERLREAADVMSANPTTLQL
                     RFLQTMLEISSERSTTTFLPLPIELFKPFLEGGRGDSSS"
     gene            12223..>12533
                     /locus_tag="VFO88_06975"
     CDS             12223..>12533
                     /locus_tag="VFO88_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545303.1"
                     /translation="MVVHTPGHVRSLAELPDERLALVAEAWSARAAAARDAGFPYVHA
                     LVNEGREAGASRGHSHSQLVWLRERPPAVAEEGGGACRVCAHLADELVGGTRVLGDRG
                     GV"
BASE COUNT         1627 a         4655 c         4559 g         1692 t
ORIGIN      
        1 ggagtggcac tcgtagccct cgtcgagggc gacggcggtg agcgtgcggc agtcggggca
       61 gcgggcgcgc aggcgcgaca cgcctagagc ctagcccgcc ggcctctccc agcggtcgat
      121 ccggtgggtc tcgcgcatcc cggcgtcgtg gtagagacgc cgcgccccgg tcgtgttgtc
      181 cgcgtcgacg tggagggaga cgtgcgtccg gtcctgccga tggaacgcgg cgaagctctg
      241 gaccaggagc gcacggccga gcccgcggcg gcgccaggcc gggacgaccc cgaggagctc
      301 gaggtagccg cccgggcccg cggcgtagcc gaccgcgacg ccggccgggc ggcccgccgc
      361 gagggccagc gtccagagct cggggtcgaa cgccgaccgg tcgagccgat ccgccgacca
      421 ccgctcgagc gtcccggcct cgtacgtcgg atcgtcggcg aacgcctcga cggcgaccgc
      481 gtggaacgcc gcctcgtcac ccggccggaa cgtgcgcgtt tcgatcccct ccggccaggc
      541 gggcggcgaa ggaggcgcct ccaggtcggt cgccatgcgg aggagcgagc gcgtggacgc
      601 ccagccgcgc gccgccagga gctccgcgca ccagaggtcg gtcgcgaaga ccccggtgac
      661 gagccgagcg gcgccgagct cgagcgcccg ctcctccatg cggcggtaga gctcgtctgc
      721 gcgcccccgg tcccgcgcct ccggatggac gcaggcgtcg acctggatct cgccctccgg
      781 cggccgccgc acctcctggg cgtaggcgac gacacggccg tcgtcttcga ccacgagcgt
      841 gtcgcgctcg agctcgaacc cctgccacgt ccagtcgtcg cgcacctcgt ccgcgtcggt
      901 tcccgtctcg ccgccgtgcg cgcggtcgta ggcgcgaacg agctccgcga tcgcctcggc
      961 gtccgcgggg gtcgccgggc ggaaggtcac ggcgggacga gcccgtcgag cgtgtccgcg
     1021 agcccggcga gcgtcgtgag ctcggccgcg cgcgggtgct cgctcgcctc gcccagccgg
     1081 ttgatccaga ccgcagggat gccgagctcg gccgcgggcc cgaggtcgtg gaacgcgctc
     1141 gcggccacgt ggacgtgccg ccccggctcc ggcgcgtgga gctcgcggaa gcgctcgaag
     1201 tggccgtgcg ccggcttgta cgagccggcc cggtcggcgg tgatcgcgtc ctcgaactcg
     1261 acgccgatcc gctcgatcga cgcggcgatg aagtcggggt cgctgttcgt gaggatcacg
     1321 agcttccagc cgcgcgcgcg aagctccgcg agctcgcccg gcacctccgg gaacgcgggc
     1381 cagcgcggga gcgactcgcc gagcgcgccc tcctcgccgg gcgggagctc gagcccctcg
     1441 ctctcggcga gcagccgcag gctctcggcg agcacgtcgc ggtaggggat cggcccgccg
     1501 agctggacgc gcggctcgat ctcgtggtag cgctcgagca gcgcctcgcg gtctgcgtcc
     1561 ggccagagcc gcccgagctc gccgccgatc ccgccgttcc agtcgatcag cgtcccgtag
     1621 cagtcgaacg tcgcgtaccg gtctccggcg cccatcgcag gccggagcct agcgagccaa
     1681 gtaacactgt gttacaagcg aacgggctcc cgcgcgccgc taggcccgga gctactcgtc
     1741 ccagcgcttg atcacgaggc aggcgttgtg cccgccgaac ccgaagttgt tcgagagcgc
     1801 gtagcggacg tcggtctcgc gcgactcgtt cgggatgtag tcgaggtcgc actcgggatc
     1861 gggatcctcg tagttgatcg tcggcgggac gatcccgtcg cggacggcga ggatgcacgc
     1921 gatcgcctcg atcgcgcccg acgcgccgag gcagtggccc gtcgcgccct tcgtcgagga
     1981 gatcggggtc gcgtacgcct tctcctcgcc gagggcgagc ttgatcacgc gcgtctcgct
     2041 cgcgtcaccg agcggcgtcg aggtgccgtg cgcgttcacg tagtcgatct cctcccgcga
     2101 gacgcccgcg gagtcgagcg ccatctggat cgcgcgcgcc gggtgccggc cggtcggatc
     2161 cggctcggtc acgtgctgcg cgtcggacga gagcccgtag ccgacgatct ccgcgtacac
     2221 cttcgcgccg cgcgcctcgg cgtgctcgag cgactcgagg acgacgatcg cgcccgcctc
     2281 gcccatgacg aacccgtcgc ggcccgcgtc gaacgggcgg ctcgcccgct ccggcgcgtc
     2341 gttgcgcctc gagagcgcgc gcatggcgcc gaagcccgcg atcccgacct cggtgatccc
     2401 ggcctcggtg ccgccggcga ggacgacgtc cgcgcggccg agccggatcg cgtccatccc
     2461 ctccccgacc gccatgttcg aggcggcgca ggcggtgcac tgggaggtga gcggtccgcg
     2521 cgtcccgagc tccatggaaa cccaggcggc ccccatgttg gggatgatcg cggggatcga
     2581 ctgcgggttc acccggtcgg cgccgcggtc cttcaaggtg tacgcgcagt cctggaagga
     2641 ctgcagcccg ccgatcccgg tggcgaccga gacgccgatg cggtcggtct cggcggcgac
     2701 gtcgagcccg gagtccgcct ccgcctggcg cgcggcggcg aggatcatct gcgcgaagcg
     2761 gtccatcttc cgcgccttgc gccgctcgat gtactccgtc gggtcgaagc ccttgagctc
     2821 gcaggcgaac ttcacgggga actcggacgt gtcgaacatc gtgatcggcc ccgccccgct
     2881 ctcgcccgcg gtgaggttct tccagtacgt ctccgggtcg ttgccgagcg gcgtcacggc
     2941 gccgaggccg gtgacgacca cccgtctccc gttgtcgatc gtgcccacga ctccctccct
     3001 acatcccgag cccgccgtcc acgagcagga cggcgcccgt cacgtagccg gcctcgtccg
     3061 agcagaggaa ccgtactgcc ccggccacgt cgtccggcga gccgagccgc cccatgggcg
     3121 tcgccgtgag caggaactcg cggatctcgt ccgagaggac ggtggtcagc tcggtctcga
     3181 tgtagcccgg cgcaacggcg ttcacgcgca cgccgcggcc gccgagctcc ttcgcgagcg
     3241 ccttcgtgaa gccgatgatc ccggctttcg tcgcggcgta gttcgtctgg cccgggttcc
     3301 cgtgcaggcc gacgaagctc gtcatgttca cgatcgcgcc cgaccgacgc ttcagcatct
     3361 tccgtgcgac ggcacggctc atgtggaacg cgccgtcgag gttcgtggac atgacctcgc
     3421 gccactcctc gtcgctcatc cgcgcgatca gcgtgtcgcg cgtgacgccc gcgttgttca
     3481 cgaggatgtc gacgtcgccg agctcggact cgacgcgctc gacgatcgcc ttcgcgctct
     3541 ccgggtcgcc gacgtcgccc tcgaccgcga tcccgccgat ctcgtccgcg atcccgctcg
     3601 cgaggtcggc gttcgagcgg tagttcacgg cgaccgtggc accggcgcgg gcgagctcgc
     3661 ggcagacggc ggcgccgatc ccgcgcgacc ccccggtcac gatcgccacg cgctcgtcga
     3721 gccggcagaa gctagacagc ggagaccgcc tcctcgagct tcgcgaggcc gtctgggtcg
     3781 ccgacggaga gcgtccgcac cgatcgatca caccggcgga cgaggcccga gaggacctgg
     3841 cccggcccga cctcgacgaa cgtctccacc ccgtccgcga cgagcgcggc gaccgcgtgc
     3901 gtgaaccgca ccggcgcggt gagctggtcg aggaggatct gcgggagcgc cgaggcgggc
     3961 tcgagcgccg ccgtcaccgt ggacatgaac gggatctccg gctcgcgcca acccgcccgc
     4021 tcgaccgcgg ggcgaagccc gtccgcggcc gacgcgacga gcggcgagtg gaacgcgccg
     4081 gtcacccgga gcttcacgac cttgcgcgcg ccgcgcccgg gcgcctcctc gacgagccgg
     4141 tcgaccgacg ccgtctcgcc cgagacgacg agctggcccg ggcagttgta gttcgccggc
     4201 cacacgccgc cgatctcgcc gcagagggcc tcgacgtccg cgtcctccat tccgatcacc
     4261 gcggccatcg cgcctgggct ctcctccgca gccgcagccg tcacctcgcc ccgcgcgctg
     4321 acgagcgcga ccgcgtcgtg atccgagagc gcgcccgacg ccgcgagcgc cgcgtactcc
     4381 ccgaccgagt gcccgagcac gacgtccggc cggacgccgg tcgtctccac cgcgcgcagg
     4441 cacgcgagcg aggtcgcgac gagcgcgggc tgttgcagct ccgtcttcgt cagctcctcg
     4501 agcggcccct cgaagcagag gcgcgcgagg tcgaagccga ccgcctccgc cgcgtcgtcg
     4561 tacaccgcgc gcgcctccgg gtacgcctcg gcgaacgcgc ggcccatccc gagctcgtgc
     4621 gagccctgcc ccgggaagag gaacgcgagc ctgctcacgc gcgctccccc gaccacgtca
     4681 tgtacgcgga gccccaggtg atcccgccgc cgacggcgct catgagcacg cgcatccccg
     4741 gccgcaggcg cccctcgtcg agcgcgtccg cgagcccgag cgggatcgac gcggccgacg
     4801 tgttcccgta ccggtcgatg ttcaggaaga ccttctcctg cggcagcccg aggttcctcg
     4861 ccgcgtgatc gatgatgcgc tggttcgcct ggtgcgcgac gtagaggtcg acgtcgtcga
     4921 tcgtcgcgcc gaccgccgcg agcaggtcgt tcgcggacga gaccatgagc cgcgtggcga
     4981 cccggaagac ctcgcgcccg ttcatctgca cccagtggag ctcgttctgc acggtctccg
     5041 cgctcgtcgg gtgccgcgag ccgcccccgg gcaggcagag gtcgaggccg tacgtcgcgt
     5101 cgacgccgag ctcgaagccg atgaagccgc cgtccgcgac cggctcgatc accgcggcgc
     5161 cggcgccgtc gccgaagagg atgcacgtcc cgcggtcgtt ccagttcacg atcttcgaga
     5221 gcgtctcggt gccgacgacg agcgcgttct ccgcgacgcc gccggagacg aggccgaacg
     5281 cctgcgagag cccgtacagg aacccgctgc acgcggcgag cgtgtcgtag ccggccgcct
     5341 tcttcgcgcc gagcgcgtcg gcgacgatcg cgccggtgct cgggaagagc atgtccggcg
     5401 tggacgtggc gacgaggacg agatcgaggt cggccgggtc gacgcccgcg cgctcgagcg
     5461 cctgccgcga cgcggggatc gcgaggtcgc tcgcggcctc gtcgtccgcg gcgatccgcc
     5521 gctcgcggat gccggtgcgc tcgacgatcc actcgtcgga cgtgtcgacc atccgctcga
     5581 gatcggcgtt cgtgagcacc ttctccggca cctggacgcc cacccccgtg atgccgaccc
     5641 gtctgccctt gccctcgacg gccgcgatca ttcgggcatg tccgtgaggg cgaagaggag
     5701 cgtcccgcgc accgcgagct cgccgtcgac cgacgcgcgc gccttcccgc gcccgaccgg
     5761 gccgcgcagc cgctccagct cgcacacgag ctcgagctcg tcgcccgggc gcacgagccg
     5821 cttgaagcgg acgtcgtcga tcccggcgaa gagcgcgagc ttgccccggt tcgccttctc
     5881 cgagaggacg gcgaccgcgc cgcactgcgc catcgcctcg accatgagga cgccgggcat
     5941 gatcgggttc ccggggaagt ggccggggag gtaccactcg tcctcgcgga cgagcttgcg
     6001 ggcgacgacg cgcgcgcccg gctcgagctc cgtcacctcg tcgaggagga ggaacggctc
     6061 ccggtggggc aggatcgcct cgatggccgc gcggtcgagc gggccgctca cccggatcgg
     6121 cccgccgacg ccgagcccgg cgcgaccgtg gagccgaggc tctcctcgag cccgtcgcgc
     6181 aggcgctgcc gcccgcggtg gacgtagcac ttcgccgtcc cgaccggcat gctcgccgcg
     6241 cgcgcgatgt cctcgtacga gaacccgagc acgtcgcgca ggacgacgac cgaccgctgg
     6301 tcggccgaca gccgcgcgag gccgtgcacg agatcggcgc ggaggtcgcc gagcagcgtc
     6361 gcgcgcagcg ggtccccgtc ctcgccggcc ggctcctcct cggtcagcgg ctcggtcgcg
     6421 cggacgcgct tgcgctcctg cgcgtcgcgg cacgtgttca cgacgagccg gtgcatccag
     6481 gtggcgaact gcgcgtcgcc gcggaactgg cggagcttcg tgcagagctt gacgagggat
     6541 tcctgcgccg cgtcgcgcgc gtcctcgagg tcgcccatga gctgcgccgc gaggctgttc
     6601 acgcgcggcg aatagcgctc gacgagcacg tcgagggccg ggcggtcgcc cgcgcgggcc
     6661 ttcgccacga gggcgaggtc gccgagcttt cggtaggcct ggatgacggg cacgggcact
     6721 ctctgctctg acagacggaa tgcgggcagg ccgggttgca cgggagcgtt agtgtagaca
     6781 gcgtggaggc cgctgaacct ctgcgcacgg acacactcga gcccgcggtc gtggagccgc
     6841 tcctcgaggg cgccttcggg cggccgtacc gctacgagcc gacggttgcc tcgacgcagg
     6901 gcctcctcgc gcccgacgac cccgagggcg ccgtcgccgc gtgcgaggag cagaccgccg
     6961 ggcgcggccg gctcggacgc acatgggagg ccccgtacgg gacggcgatc ctcgtctcga
     7021 tcctccttcg cccgccggcc ggccgccgcg tgcaggagct ctcgctcgtc gccgggatcg
     7081 cgacagcgct caccgcggag cgggcgctcg ggctcgcgag ccaggtgaag tggccgaacg
     7141 acgtcctcgt caaccggagc aaggtcgcgg gcgttctcgc ggaggcgcgc gacgacgcgg
     7201 tcgtgctcgg catcggcctg aacgtgaacc agcggcggga cgagctgccg tcgggcggga
     7261 caccgcccgc cgcctcgctc cgcacgatcg acgcggtcgt gcgcgagcgg gcgccgctcc
     7321 tcgcgctcct cctcgccgag gtcgagaagg cgtacgcgcg ctggcgcgag ggcgggctcg
     7381 ccgagctcta cgtcgacctc ggcccgcgcg acttcctccg caaccggcgc gtgcgcgtcg
     7441 gggacgaggc cggcctcgcg atcgcgatcg accgcgaggg gcggctcgag gtcgacgtcg
     7501 gcggcgagcg ccgcctcgtc gagagcggcg aggtgacgta cctccgctag ccgacgcggc
     7561 gcgagacggg cttgcccgcg ttgccggcga gatcgaacga gctcacggtc acggaggtgc
     7621 cgcgcaccgg gaggcggaac gtccaggtcc cgcgcgcgag cgaccggttg aacgtcctgc
     7681 cgccgaggcg gatcgcgagc ctcacgtcct cggagaccga gagccggagc gacgtgccgc
     7741 cctgcacgcg ccgggcccgg aggatgcgcg tgaccggctt cgtcgtgtcg acgacgacct
     7801 tcgccgtcag gcgccgcgtg ccgaggctcg cggtggtcgc atcgacgatc acggtgtacg
     7861 tccggtcggc cgcgggcgag ccgccgggct ccctgccgtc ccagacgaag ctcttcttgc
     7921 cgagcgaaag ggagccgtct cggagcgtgg ccaccctgcg ggagccggcc atgacgcgcg
     7981 cgtcgacgga tgcggcgcgc ttgagctcga agccgaccgt cagcgtgtcg agcgttccgt
     8041 ccccgttcgg ggagaagggc gtcggcgtcg cgctcaggtg ggcgagcgtg cggtcgacgc
     8101 ggaccgcggc tgtcctcgta accgtctcgg ggcccgacgt cgcctcgacc acgagcacgt
     8161 agcggccgtc ggggaccttc acgccgttcg cgtccttgcc cttccacccg acgagccggg
     8221 cgcccgcgcc gagcgcccgg ttcgcaacga gcgtcgtgac cggctcgccc tcggaggtct
     8281 cgagccgcgc ctcgacggtt gcgctccggg tgagcgtgaa gtggatccgt tggcggtcgc
     8341 ccacgccgtc gccgttcggc gtcgtgacga cgggcttcgg cgcgagcgcg cccagctcga
     8401 gcggcggctc gctgcccccg acgacgccgg tcgcggcgcg gacgccctcg ccggcggcga
     8461 tcgcgtaggt gtagcggccg ggcggcgcac ctgcggagcc ccacgtccag tccaccgcct
     8521 tcgtcgtccc ggagccgcct gccacgcggt tcccgaccgc gtcgaggacg tcgaccgtcc
     8581 acccgcgcgc ctcggagagc gtcgccgtga accggaccgg cccgccgagc gcgcccgtcg
     8641 agcgcgggct gaagatcttc gggccgccga gcgcgcgggc cgcggccgcg agcgagccga
     8701 gctcgccgtg cagcctcgcg cccgggcagg aggtcgagcc ggtgtcccgg tggccggaga
     8761 ccgcgttcag ggagacgggc gtcccggccg ggaacttcgg gttccccccg gacgtccacg
     8821 tgagccggga ggccgggtcg acgtgcgcga cgtcgagccg ccacgcgagc agcgcggtga
     8881 gcgcggtgcg ggccttcgcg gagatcgtcc cgccctcgta cgtgccgagc accgcgatcc
     8941 cggtgctccc ggtgttgaag ccctgggcgt gggcgccgat cacgttcttc gccgcgccgc
     9001 ccttgcgccc gacgaagatc tgcccgaacg ggtcgacgag gaagttgtag gcgatgtcgt
     9061 tccagccgtt cgagagcacg tggtacgact ggatcccgcg cacgatcgcg gccgactgcg
     9121 cggcggaggt cggcttcgag ccggccgtgt ggtggacgac cgcgaacgag agccgcgagg
     9181 cgtacgacgg cgttccgcgc acgatcgact cgttcgcgcc ccaggcggcg cgcttcacga
     9241 gcgcgggctg cggcggggcg gcggcgagcg tacgggcagc cggctcggcc gcgcgcggcg
     9301 cctcgcgcac ggggctcgtc acgtagtggg cacgcagccg cgtgacgcgg ccgctcaccc
     9361 gcacctggag ccggcgcgct gcgcccgtcc accacgggtt cccgagctcc cagccggcct
     9421 tcgcccgggc ctcccggtcg cccgcgttcg ggccgtcctc cgtctccggg cgggccggcc
     9481 gccaggcgct ccacgcccca ccgatccgcg ccgtgcggaa ctcggcgtcg cccggcccct
     9541 gccagtggag cccgacgagg tggaaccgcg cgggcgccgt ctgggcgggc agcacgcgga
     9601 tcgccccggc cgcggcgcgc tcggccccgc ttccggcgag cggaacgtcg cgcgaggcga
     9661 cgctcacccc ggccgaggcg ggcccggcgc aggcgagcgc caagagagcg gcgagaagtg
     9721 tgagacgcgg cactgccatc cgggggcgga acgacgaacg aagcaaagat cggcaggacg
     9781 ggcaagagac taaaagccct gcagatacaa tccttacagg gtcgacatgc gtttcctccg
     9841 tctcctgtcc gtcgcggcgc tcctcacggg gacggccctc gcgctcgccg gagccgcgct
     9901 ctcgcaggag gacggcgacc gcccgcgcgt gctcgcggtc gagttcgcga acgacgtgaa
     9961 cccggtcacg caggactacc tgaccgacgc gatcgaccgg gcgaacgacg aggagttcga
    10021 cgcggtcgtg atcctcatgg acacgcccgg cgggctcgac tcctcgatgc gcgagatcat
    10081 ccaggccgag ctcgactccg aggtgcccgt cgtcgtctac gtctatccgc ccggctcgcg
    10141 ggccgcctcg gccggcgtct tcctcgcgat ggcggccgac gtggccgcga tggcgccgca
    10201 gacgaacatc ggctcgtcga ccccgatcgc gacgagcggc gaggagatcc cggaggacct
    10261 ccgccgcaag gtcgtgaacg acgcggctgt cttcgtgcgc gagctcgccg aggagcacgg
    10321 ccggaacggc gagtgggccg agagcgccgt ccgccgcgcg tcgaacatcg gcgcccgcga
    10381 ggcgctcgag gagaacgtcg tcgacttcgt ctcgcccgac ctcgagacgc tgctcgagga
    10441 catggacggc ttcgagacga agccgaaggg gatcgtcatg aacaccgctg gggcggaggt
    10501 cgtgaacgtc gagatgggcc tctggaagca gatcctcgac ctgctcgtcg acccgaacct
    10561 catcgtcctc ctcatgtcga tcgggatcct cgggatcacg atcgagatcc tgaacccggg
    10621 cctcatcttc ccggggacgg tcggcgcgat ctcgctcatc atggggctct tcgggctcca
    10681 ggtcctgccg atcagctggg ccgggctcct gctgctcctc ctctcgttcg tcttcttcgc
    10741 cgcggaggcg ttcgtcgcga gccacggcgc gcttgccctc gcgggggcgg tcagcttcgt
    10801 gttcggctcg ctcctcctct tcgaccccgc gggagaggcg tacgccgtct cgctgtgggt
    10861 ctcgctcgcg atcgcgggga cgctcgcgct cgtgctcggc gtcgcgctcg gcaaggccgt
    10921 gcagctccgc cggacgaagc cgcagacggg gcaggacgag atgctcggcg cggtcgggat
    10981 cgtccgccac gcgctcgacc cggacggcta cgtcttcgtc cacggggagc tctggcgcgc
    11041 ccgggtggac aacggcccgg tcgagccggg gacgcgcgtt caggtcgacg tggtccgcga
    11101 cgacctcgtg ctcgaggtga gcccggtcga gacgcccgtc ccggcggcac ccgccggcgg
    11161 caccgcatag gatggcctca tgtcgcctgc gctgatcgcc gtcgcggcgg tcctcgtcgt
    11221 cctcatcttt ctcctctcgg cgtccattcg tgtcgcccgg gagtacgagc gcggcgtcat
    11281 cttccggctc ggccggctga agagcccgcc gaagggcccg ggcctcttcc tgctcattcc
    11341 ggtgatcgac aagatggtgc gggtcgacct gcggacggtg acgctgaacg tcccgccgca
    11401 ggaggtgatc acgaaggaca acgtgcccgt gcgcgtgaac gcggtcgcgt acttccggat
    11461 catcgacccg gagcgggcga tcgtgcaggt cgagaactac atggtcgcga cctcgcagat
    11521 ctcgcagacg acgctgcggt cggtgctcgg ccagcacatg ctcgacgagc tcctctcgga
    11581 gcgggacaag atcaacgaga tcctccagcg gatcatcgac gaggccacgt cgccgtgggg
    11641 cgtcaaggtc tcgatcgtcg aggtgaagga cgtcgagatc cccgccggga tgcagcgcgc
    11701 gatggcccgc caggccgagg ccgagcgcga gcggcgcgcg aaggtgatcg cggccgaggg
    11761 cgagttccag gcctccgagc ggctgcgcga ggcggccgac gtcatgagcg cgaacccgac
    11821 cacgctccag ctgcggttcc tccagacgat gctcgagatc tcctccgagc ggtcgaccac
    11881 gaccttcctg ccgctgccga tcgagctctt caagccgttc ctcgagggcg gccgcggcga
    11941 ctcctcgagc tgacgagcgc ctcggctccc gacctgcgcc gcgacccgat ctcgggccgg
    12001 ctcgtcgcga tcgcgccggg gcgcgcccgg cggccggggg cgcgaacgcc ggacacgttc
    12061 gacccggccg agggggagga ctgcccgttc tgcgagggcc acgagggacg aacgccgcct
    12121 gaggcgctcg cgctcggacg gcctgacggc gcgccggcgg actcgccggg ctggcaggta
    12181 cgcgtcgttc cgaacctcta cccggcgctc gagcggcagg aggtggtcgt ccacacgccg
    12241 ggacacgtcc gctcgctcgc ggagctcccc gacgagcggc tcgcgctcgt ggcggaggcg
    12301 tggtcggcgc gcgcggcggc cgcgcgtgac gccggcttcc cgtacgtcca cgcgctcgtg
    12361 aacgaggggc gcgaggcggg cgcgtcgcgc gggcattcgc actcgcagct cgtctggctc
    12421 cgcgagcggc cgccggcggt ggcggaggaa ggcggcggcg cctgccgggt gtgcgcgcat
    12481 ctcgcggacg agctcgtcgg cggcacacgc gtgctcggcg accgcggcgg cgt
//
LOCUS       DASTFC010000235         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_235989,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000235 DASTFC010000000
VERSION     DASTFC010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235989"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFO88_06980"
     CDS             complement(<1..753)
                     /locus_tag="VFO88_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET9545304.1"
                     /translation="MSDRPRAAILLTGSELARGSIRDANGPFLARELTRLGLEPARWT
                     LVGDAPEELEAALRDGLAADLCVVSGGLGPTHDDRTVELLARATGRALHVDAGLEEEI
                     DGVARAVAGRLGRPYAELAVGVRKQASLPEGAVSLGLAGTAPALLLEHADGRVAVTLP
                     GPPGELRRLWPRVVESAPVQALASRARRREQRVLRFFAVPEAAVARAVEHAGGEGDGL
                     ELTICARNFELEVDLFVSAGGEERAAAVEDALV"
     gene            complement(750..1499)
                     /locus_tag="VFO88_06985"
     CDS             complement(750..1499)
                     /locus_tag="VFO88_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET9545305.1"
                     /translation="MFEIGNSLREARLRQGLDLARVEDDTKIRAKYLQGLEDERFEVL
                     PSETYVKGFLRTYAEYLGLDGQLYVDEYNSRFSAAEEPPIASPPTRRRRRRAAESNLV
                     VAALAGIVAVAVLVVVAFTLSEGQPDVSPTAGLGPGTTTGAQPETTTEETGTAPPPAN
                     RASVALVLVASRGDCWVTVRRGAPNGDVVFSGTIYQGETQRFTGRRLWLEFGDAPSEN
                     LNVRLNGRRVRDFPVGEPVVIVTPEGVRAQD"
     gene            complement(1570..1914)
                     /locus_tag="VFO88_06990"
     CDS             complement(1570..1914)
                     /locus_tag="VFO88_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545306.1"
                     /translation="MRNPVAFLALLVALLALGAVAGTGYVAQTRFEVSWLEAAYGIPV
                     VVVLALLALVLANRARMRHQRTLGRSGGTAIAGLARVVAGLALLCAAAAVVALIVFFV
                     LVQTDGLTEAPW"
     gene            complement(1942..>2483)
                     /locus_tag="VFO88_06995"
     CDS             complement(1942..>2483)
                     /locus_tag="VFO88_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9545307.1"
                     /translation="TLPEFIRDIPFVGAAIGNLSVMTWLIILIVVLAWAVIFKTPIGL
                     RLRSVGEHPRAAETVGISVYKIRYGAVILSGILASLGGAYISFGFGGSFNENMSGGRG
                     FIALAAVILGKWHPVGAFGACLLFGFASGLAQRLQGEADISANLLSTLPYVLVLIALV
                     GVIGRSRPPAAVGRPYVKQ"
BASE COUNT          360 a          930 c          908 g          285 t
ORIGIN      
        1 cacgagcgcg tcctcgacgg ccgccgctcg ctcctccccg cccgccgaca cgaagaggtc
       61 gacctcgagc tcgaagttgc gcgcgcagat cgtgagctcg agcccgtccc cctccccacc
      121 cgcgtgctcg accgcccgcg cgacggccgc ctcgggcacg gcgaagaagc gcaggacccg
      181 ctgctcacgt cggcgcgcac gcgaggcgag cgcctgtacg ggcgccgact cgacgacacg
      241 cggccagaga cggcggagct ccccgggcgg gccgggcagc gtcacggcga cgcgcccgtc
      301 cgcgtgctcg aggagaagcg cgggcgccgt tcccgcgagg ccgagcgaga ccgcgccctc
      361 ggggagcgac gcctgcttcc gtacgccgac cgcgagctcg gcgtagggcc gcccgagccg
      421 cccggcgacc gcccgcgcga cgccgtctat ctcctcctcg agccctgcgt ccacgtgcag
      481 cgcccgcccg gtcgcgcgcg cgaggagctc gaccgtgcgg tcgtcgtgcg tcggcccgag
      541 cccgcccgag acgacgcaga ggtcggccgc gagcccgtcg cggagcgccg cctcgagctc
      601 ctcgggcgcg tcgccgacga gcgtccagcg cgcgggctcg aggccgagcc gggtcagctc
      661 ccgcgcgagg aacggcccgt tcgcgtcgcg gatcgagccg cgcgcgagct cgctccccgt
      721 caggaggatc gcggcccgcg gccggtcgct caatcctggg ctcggacgcc ctcgggcgtc
      781 acgatcacga ccggctcgcc gacggggaag tcccgcacgc gccggccgtt cagccggacg
      841 ttcaggttct cagagggcgc gtcgccgaac tcgagccaga gccgccggcc ggtgaaccgc
      901 tgggtctcgc cctggtagat cgtcccggag aagacgacgt cgccgttcgg ggcgccgcgc
      961 cggaccgtca cccagcagtc gccgcggctc gctacgagca cgagcgcgac gctcgcgcgg
     1021 ttcgcgggcg gcggcgccgt accggtctcc tccgtcgtcg tctccggttg cgccccggtc
     1081 gtcgtcccgg gcccgaggcc cgcggtcggc gagacgtccg gctggccctc cgagagggtg
     1141 aacgcgacga cgacgagcac cgccacggcg acgatcccgg cgagcgcggc gacgacgagg
     1201 ttcgactcgg cggcgcgacg gcgccggcgg cgcgtgggcg gcgaggcgat cggcggctcc
     1261 tccgccgcgg agaagcggga gttgtattcg tcgacgtaga gctgcccgtc gaggccgagg
     1321 tactccgcgt acgtgcggag gaagcccttg acgtacgtct cggagggcag cacctcgaag
     1381 cgctcgtcct cgagcccctg gaggtacttc gcgcggatct tcgtgtcgtc ctcgacgcgc
     1441 gcgaggtcga gcccctggcg gagccgggct tcacgcaggc tgttgccgat ctcgaacatg
     1501 gcctctaggg ggtggtgcgg cggagcagct tgcgattgta gcttccgtgg cgaccgacca
     1561 cgtaggtcgc taccacgggg cctccgtgag cccgtccgtc tggacgagca cgaagaacac
     1621 gatcagggcc acgacggccg cggccgcgca gaggagcgcg agccctgcga cgacccgcgc
     1681 gagccccgcg atcgccgtcc cgcccgaccg cccgagcgtg cgctggtgcc gcatccgtgc
     1741 gcggttcgcg agcacgaggg ccaggagggc gaggacgacg acgaccggga tcccgtacgc
     1801 cgcctcgagc cagctcacct cgaaccgcgt ctgcgcgacg taccccgtgc cggccaccgc
     1861 accgagcgcg agcagggcga cgaggagcgc gaggaaggcg accgggttcc gcaccggcgg
     1921 cgactctacc ggccgcgctg gctactgctt cacgtacggc cgcccgacgg ccgccggcgg
     1981 gcgcgagcgc ccgatcacgc cgacgagcgc gatcagcacg agcacgtacg gaagggtcga
     2041 gaggaggttc gcggagatgt ccgcctcgcc ctgaagccgc tgcgcgaggc cgctcgcgaa
     2101 gccgaacagg aggcacgcgc cgaacgcgcc gaccgggtgc cacttcccga ggatgacggc
     2161 cgcgagcgcg atgaagccgc gcccgccgct catgttctcg ttgaacgagc ccccgaagcc
     2221 gaacgagatg tacgccccgc cgagcgacgc gaggatcccc gagaggatga cggccccgta
     2281 gcggatcttg tagacggaga tccccaccgt ctcggcggcg cgcgggtgct cgccgaccga
     2341 gcgcagccgc aggccgatcg gcgtcttgaa gatgacggcc caggcgagga cgacgatgag
     2401 aatgatgagc cacgtcatca cgctgaggtt cccgatcgcc gccccgacga acgggatgtc
     2461 gcggatgaac tccggcagcg tca
//
LOCUS       DASTFC010000236         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_565904,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000236 DASTFC010000000
VERSION     DASTFC010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565904"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..812
                     /gene="rlmB"
                     /locus_tag="VFO88_07000"
     CDS             87..812
                     /gene="rlmB"
                     /locus_tag="VFO88_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET9545308.1"
                     /translation="MARELVYGRRPVREVTRAGRRRILELLVTERALRAEPWLEEVDG
                     VRPLRRPESALSEAAHTRDHQGVVAFCEPYAYADAYELASRDRPLIVCLDQVTDPHNL
                     GAVCRTAEAAGATGVVLPERSSARVTPAVCRASAGAVEHLPVAVVVNLGRYLEETKRG
                     DLWAWAAAGDAPTPVWEADLAGGTFLVLGSEGKGLRPGVRRACDDAFSIPLAGRVESL
                     NVSVAAGIALFEAVRQRGGTPSS"
     gene            967..1416
                     /locus_tag="VFO88_07005"
     CDS             967..1416
                     /locus_tag="VFO88_07005"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9545309.1"
                     /translation="MPDPTLYLFDGWNILRAGSFRSREELVDRLTGFVALHGARGVVV
                     FDGAGEDATLGALEVRFAPHADELLERLAVAARDTPGERVVLVSSDRDVRDTAGQEVR
                     RWASAGFVEELERTARDPGRALPEARIEDLLDPETRARLERIRRGNA"
     gene            1518..2180
                     /gene="sigH"
                     /locus_tag="VFO88_07010"
     CDS             1518..2180
                     /gene="sigH"
                     /locus_tag="VFO88_07010"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HET9545310.1"
                     /translation="MPRQQTATVRPPERLKREQDDVRLVLRARNGDDAALDALIRRYT
                     GFVRLKASSYFLAGGESDDLIQEGLVGLYKAVRDFRVDKETSFRSFAELCITRQIITA
                     IKAATRFKHQPLNGYVSFSQTPAGQDDSDCTLGDALPGPGVDDPAICVISTEELQSLV
                     FALGTGLSPLEADALRLYMEGLSYEEMSEELGCDTKTIDNALQRVKRKVLNHQRERQV
                     LL"
     gene            2208..2280
                     /locus_tag="VFO88_07015"
     tRNA            2208..2280
                     /locus_tag="VFO88_07015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2241..2243,aa:Thr,seq:tgt)
     gene            2350..2613
                     /locus_tag="VFO88_07020"
     CDS             2350..2613
                     /locus_tag="VFO88_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545311.1"
                     /translation="MRNLTEIRKELDEATERRAALWEALSEGFDAEQSAEAARLSKRI
                     EELWVEARIARAHARYGPSDEIITRARAHDRLDREARRWREAA"
BASE COUNT          378 a          970 c          924 g          374 t
ORIGIN      
        1 gccgaccggc tccgcggcga gatcgacgcc gccgggtggg aggtgcagga cgtggccgac
       61 ggcttccgcc tcgtcccccg ttcgtagtgg cgcgcgagct cgtctacggg cggcgccctg
      121 tgcgcgaggt gacgcgggcg ggacgccggc ggatcctcga gctgctcgtc accgagcggg
      181 cgctgcgcgc ggagccgtgg ctcgaggagg tcgacggcgt ccggccgctc cggcgcccgg
      241 agtcggccct gagcgaggcc gcccacacgc gcgaccacca gggcgtcgtc gccttctgcg
      301 agccatacgc gtacgcggac gcctacgagc tggcctcccg cgaccgcccg ctgatcgtct
      361 gcctcgacca ggtcacggat ccgcacaacc taggcgccgt ctgccgcacc gccgaggcgg
      421 ccggcgcgac cggcgtcgtg ctcccggagc ggagctcggc ccgcgtcacg cccgccgtct
      481 gccgcgcctc ggcgggcgcg gtcgagcacc tcccggtcgc ggtcgtcgtg aacctcggcc
      541 gctacctcga ggagacgaag cgcggcgacc tctgggcctg ggcggccgcc ggcgacgcgc
      601 cgaccccggt ctgggaggcc gatctcgcgg gcgggacgtt cctcgtcctg ggctccgagg
      661 ggaagggcct tcgccccggc gtccggcgcg cctgcgacga cgccttctcg atcccgctgg
      721 ccggccgcgt cgagtcgctg aacgtgagcg tcgccgccgg gatcgcgctc ttcgaggccg
      781 tccgccagcg cggtggaacc ccgagctcgt aacacagtgt cactaaccgt cggggccacc
      841 gaccggccgc ggcgagaccc gttgtcctag taacactgtg ttactaagag aacgggctcc
      901 ccgcacggcc ggccccggcg tgccggacgg ggtgccccgc acggcccctg tggaaagcga
      961 gcagccatgc ctgatccaac gctctacctc ttcgacgggt ggaacatcct ccgcgccggc
     1021 tcgttccgct cgcgcgagga gctcgtcgac cggctgaccg gcttcgtcgc gctccacggc
     1081 gcgcggggtg tcgtcgtctt cgacggagcg ggggaggacg cgacgctcgg cgcgctcgag
     1141 gtgcgcttcg cgccccacgc ggacgagctg ctcgagcgtc tcgcggtcgc cgcccgcgac
     1201 actcccggcg agcgggtcgt cctcgtctcc tccgaccgcg acgtccggga caccgccgga
     1261 caggaggtcc ggcggtgggc gtcggccggc ttcgtcgagg agctcgagcg aaccgcccgc
     1321 gaccccggtc gcgcgctccc ggaggcgcgg atcgaggacc tgctcgaccc ggagacccgc
     1381 gcacggctcg aacgcattcg ccgcggaaac gcttgatatc tcctcgtacc cctgctacct
     1441 tcacgtcacc ttcaacctga gggttagggt tgccgggacg tacgggcacc ccttcccgcc
     1501 gccaagagga gagcgaaatg cctcgccagc agacggcaac cgtacgtccc cccgagcgac
     1561 tcaagcgcga gcaggacgac gtccgcctcg tgctccgtgc gcgaaacggc gacgacgccg
     1621 cgctcgacgc gctcatccgc cgctacaccg ggttcgtccg cctgaaggcg agctcgtact
     1681 tcctcgcggg gggcgagtcg gacgacctga tccaggaggg gctcgtcggc ctctacaagg
     1741 ccgtccgcga cttccgggtc gacaaggaga cctcgtttcg ctcgttcgcc gagctctgca
     1801 tcacgcgcca gatcatcacg gcgatcaagg cggcgacgcg cttcaagcac cagccgctga
     1861 acggctacgt ctccttcagc cagacgccgg cggggcagga cgactccgac tgcacgctcg
     1921 gcgacgcgct ccccggcccc ggcgtggacg accccgcgat ctgcgtcatc tcgaccgagg
     1981 agctccagag cctcgtcttc gcgctcggca ccggcctctc gccgctcgag gcggacgcgc
     2041 tccgcctgta catggagggg ctctcgtacg aggagatgtc cgaggagctc ggctgcgaca
     2101 cgaagacgat cgacaacgcg ctccagcgcg tgaagcggaa ggtcctgaac caccagcggg
     2161 aacggcaggt cctgctgtag ggaagccggg ctacgatgag gtcgtaggcc ggtgtagctc
     2221 aatcggtaga gcagcgctct tgtaaggcga aggttggagg ttcgagtcct ctcaccggct
     2281 ctctcgggat gcgcgtttgc gcgacggctg tgcgggaatc gagccgagcc gcgggacgtt
     2341 atccctggtg tgagaaacct gaccgaaatc cgcaaggagc tggacgaggc caccgagcgc
     2401 cgagcagcgc tgtgggaggc tctgtccgag ggcttcgacg ccgagcagtc cgcagaggcc
     2461 gcccgcctct cgaagcggat cgaagagctc tgggtcgagg ctcggatcgc gcgtgcccac
     2521 gcgcgctacg gcccctcgga cgagatcatc acgcgtgcgc gtgcccacga ccgcctcgat
     2581 cgcgaggcgc gtcgctggcg cgaagccgcc tgactccatc tccaccttcc agctgggggc
     2641 cgtcga
//
LOCUS       DASTFC010000237         6067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_994468,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000237 DASTFC010000000
VERSION     DASTFC010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994468"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFO88_07025"
     CDS             <1..604
                     /locus_tag="VFO88_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET9545312.1"
                     /translation="GEDIHAATASEVLGIPQAELTKDERNVAKMVNFGIIYGISAFGL
                     SENLEIPREEAQRYIDAYLARFPLVQDFIQRTIEQATTDGYSTTLLGRRRPAPELRAS
                     NRQTRGFGERLAVNFVMQGTAADIIKKAMVSIHRRLREEGRAARLVLQVHDELLLEVP
                     DVEVSAAKELVREEMCAAYPLDPPLAVDVGTGQDWNEAKT"
     gene            693..2381
                     /locus_tag="VFO88_07030"
     CDS             693..2381
                     /locus_tag="VFO88_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9545313.1"
                     /translation="MRRIAASVGLLLTLIAVAAIGVGAAGPGPQGSPPPKAKHTASIQ
                     LLTISDWHGQLTPVSGVGGAAFLKTYFDEARAENPNTLLFTAGDAIGATPPISSFFED
                     EPAIKTLGMMGLSADTFGNHNFDRGVEHLQRMIDLAEYPFVAANLANLEENLTGVEPY
                     TIFALDGVRIAVIGIVNEEAPTLVTPGALGTLEITDSVEAANRTAFQARQFGRADVVV
                     VLTHKGIRGRDAGGNAFGELIDFADAVDPGLIDVIVGDHTDFEYSGVHQGSILAVENR
                     SKGIQFAKIQLEVDRRVGSVLSKSVSFVTPTNAGVTPDPAIQAYIDELNAQLAPILGT
                     VIGSSTRAIPRSDSCGRADSRLCESLVGNVVTDALRTAYGTDFAITNSGGLRAPLTCP
                     EAGPSGFCPTGITPPPHPITRGAVLGVLPFGNISVTLTVTGAELKAFLENGVSRMPAA
                     DGRFPQVSGLCFTYDIAAPAGSRVTSAVRQAADGSCTGPAVDLTAGASYSLTTNDFVA
                     HGGDGYPDVYSKSTTRNIMDEDAAAYVAANSPLSPSIQGRIVCTGAACPAITAP"
     gene            complement(2386..2919)
                     /locus_tag="VFO88_07035"
     CDS             complement(2386..2919)
                     /locus_tag="VFO88_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545314.1"
                     /translation="MRVVLATLLLALVAAGAAHAHTTGIHTGFVSTVSGIDPPLPGLL
                     VRVVAGHERLSVQNWTERTVVLYDEDGGVAAQLAPGEGHAWADPRIDWEGPPPEGETR
                     LRDWRIEGEADGERFAIAGFLGYRPPEAGAEEDEAAGGAGWPGWATAAVLVGGGVLAA
                     VALALPLVRRKGEGVRP"
     gene            complement(2938..3939)
                     /locus_tag="VFO88_07040"
     CDS             complement(2938..3939)
                     /locus_tag="VFO88_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545315.1"
                     /translation="MPRNPASYGRFRDALIVAGVLVLAGVALADAVRGGEDAVEPAAA
                     TGSTETATVDDGSGRLAGVPAPGSLVLLDDDGCTLRQIVVSGGQELPLPRIETGCELW
                     AAPRGGLVAYALEGPWRAGAFRPFRLLDLSHPDEPLGTYQTFNGVTWSADGQHAFWCH
                     DPGRGYDLPVRTAERPRALRGCPVGYAPGERPAFADGGRLLVGGEPVLEAPERIVRAT
                     FNRDDTVTLFLADGVIGRYRGGEPLGRVRAPATTGPPALSADGCDLAFPVAPASVVLG
                     SVCAGVQGTFEGRAAAWSPDGEWLAVAEGEEIVLHHLAGTLAMARWPIAVRALAWVR"
     gene            3964..5319
                     /gene="rpsA"
                     /locus_tag="VFO88_07045"
     CDS             3964..5319
                     /gene="rpsA"
                     /locus_tag="VFO88_07045"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9545316.1"
                     /translation="MTERTVTEADPTEYDADVWTRGPDGELVPNYDATFPTINEGEVV
                     SGKVVRVDKDEVLVDIGYKSEGVIPVAELSIRRSVDPADEVQLGDDVDALVLTKEDAE
                     GRLILSKKRARFEMAWKRIEGAAESGEPVEGVVIEVVKGGLILDLGVRGFLPASLVDI
                     RRVQDLEDFLGQTLRCKVIELNRSRNNVVLSRRAVLEEERKEARQAILDRLSPGNVVQ
                     GTISNIVDFGAFVDLDGIDGLIHISELSWSHVNHPSEVLDIGQEVEVKVLDIDRDRQR
                     ISLGLKQTQSDPWQQVVETHGEGDVVEGRVTKVVTFGAFVEILPGVEGLVHISELANH
                     HVENPREIVNQGEDVRAKIIEMDADRRRLSLSLKRVEGDGAAADPSELGLSEEVFTED
                     EAALEDEPAAEAEVEPELEAEVEPEVEAAEEPEAEAAAEPEAEAEAGAEPAEPAEDEQ
                     A"
     gene            5425..5991
                     /gene="coaE"
                     /locus_tag="VFO88_07050"
     CDS             5425..5991
                     /gene="coaE"
                     /locus_tag="VFO88_07050"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET9545317.1"
                     /translation="MTGSIGAGKSEVLAAFERHGAAVISSDDIVHRLLREDPAVKGAI
                     VERLGERVLGEDREIVRSRVAELVFSDAEALRWLEELLHPRVVTTYLRWREDLRAGAD
                     PPDVCVTEVPLLYEVGAEERFDAVVAVSASPEVRAARLRHPVSDRETRLLPDAEKLAR
                     ADFAYVNEGSLEELDRFVSDVMGTLTRS"
BASE COUNT          888 a         2216 c         2204 g          759 t
ORIGIN      
        1 cggcgaggac atccacgcgg ccacggcctc ggaggtgctc gggatcccgc aggccgagct
       61 cacgaaggac gagcgcaacg tcgcgaagat ggtgaacttc gggatcatct acgggatctc
      121 cgccttcggg ctctcggaga acctcgagat cccgcgcgag gaggcgcagc gctacatcga
      181 cgcgtacctc gcgcgcttcc cgctcgtgca ggacttcatc cagcggacga tcgagcaggc
      241 gaccacggac ggctactcga cgacgctgct cggccggcgg cggcccgcgc ccgagctccg
      301 cgcctcgaac cgccagacgc gcgggttcgg cgagcggctc gcggtcaact tcgtcatgca
      361 ggggacggcc gcggacatca tcaagaaggc gatggtctcg atccaccggc gcctgcggga
      421 ggagggccgg gcggcgcggc tcgtcctcca ggtgcacgac gagctgctcc tcgaggttcc
      481 cgacgtggag gtctcggcgg cgaaggagct cgtgcgcgag gagatgtgcg ccgcctaccc
      541 gctcgacccg ccgctcgcgg tcgacgtcgg caccggccag gactggaacg aggcgaagac
      601 gtaacggacc ggtcgcgaaa ccgcgccggc cgttgaaaaa ccgcggccgc tgcgggagga
      661 ttcctccacc gggactacga gaggagacct cgatgagacg tattgcggcc agcgtcggac
      721 tgctcctgac cctgatcgcg gtcgccgcga tcggcgtggg cgccgccggg ccggggcccc
      781 agggctcgcc gccgcccaag gcgaagcaca cggcgtcgat ccagctcctg actatctccg
      841 actggcacgg ccagctcacg ccggtcagcg gggtcggagg cgcggcgttc ctgaagacgt
      901 acttcgacga ggcgcgtgcg gagaacccga acacgctcct cttcaccgcg ggcgacgcga
      961 tcggcgcgac gccgccgatc tcgagcttct tcgaggacga gcccgccatc aagacgctcg
     1021 ggatgatggg cctctccgcc gacacgttcg gcaaccacaa cttcgaccgc ggcgtcgagc
     1081 acctccagcg gatgatcgac ctcgcggagt acccgttcgt cgccgcgaac ctcgccaacc
     1141 tcgaggagaa cctcacgggc gtcgagccgt acacgatctt cgcgctcgac ggcgtacgga
     1201 tcgccgtgat cggcatcgtg aacgaggagg ccccgacgct cgtcacgccg ggcgcgctcg
     1261 ggacgctcga gatcaccgac tccgtcgagg cggcgaaccg cacggcgttc caggcgcggc
     1321 agttcggccg cgcggacgtc gtggtcgttc tcacgcacaa ggggatccgc ggccgcgacg
     1381 ccggcgggaa cgccttcggc gagctcatcg acttcgccga cgcggtcgac cccggcctga
     1441 tcgacgtgat cgtcggcgac cacacggact tcgagtacag cggcgtccac cagggctcga
     1501 tcctcgcggt cgagaaccgc agcaagggca tccagttcgc gaagatccag ctcgaggtcg
     1561 accggcgcgt gggcagcgtg ctctcgaagt cggtctcctt cgtgaccccg acgaacgcgg
     1621 gcgtcacgcc cgacccggcg atccaggcgt acatcgacga gctgaacgcc cagctcgcgc
     1681 cgatcctcgg caccgtgatc gggtcgtcga cgcgggcgat cccgcgctcc gactcgtgcg
     1741 gccgggccga ctcgcgcctc tgcgagtcgc tcgtggggaa cgtcgtcacg gacgcgctcc
     1801 ggacggccta cggcaccgac ttcgcgatca cgaactcggg cggcctgcgc gcgccgctca
     1861 cgtgtcccga ggcggggccg agcgggttct gcccgacggg catcacgccg ccgccgcatc
     1921 cgatcacgcg cggcgccgtg ctcggcgtcc tcccgttcgg gaacatctcg gtcaccctca
     1981 ccgtgaccgg tgccgagctc aaggcgttcc tcgagaacgg cgtctcgcgg atgccggcgg
     2041 ccgacggccg cttcccgcag gtctccgggc tctgcttcac gtacgacatc gcggcgcccg
     2101 ccgggagccg cgtgacctcc gccgtgcggc aggccgcgga cgggagctgc accggcccgg
     2161 cggtcgacct gacggccggc gcgtcgtact cgctcacgac gaacgacttc gtcgcgcacg
     2221 gcggcgacgg ctacccggac gtctactcga agtcgacgac gcggaacatc atggacgagg
     2281 acgcggcggc gtacgtggcg gcgaactcgc cgctcagccc gtccatccag ggccgcatcg
     2341 tctgcaccgg cgcggcctgc cccgcgatca ccgcgccgtg aggcactagg gccgcacgcc
     2401 ctcgcccttc cgcctgacga gcgggagggc gagggcgacg gccgccagga cgccgccgcc
     2461 gacgagaacc gcggccgtcg cccagcccgg ccacccggcc ccgccggccg cctcgtcctc
     2521 ctccgccccg gcctcgggcg ggcggtagcc gaggaagcct gcgatcgcga agcgctcgcc
     2581 gtccgcctcg ccctcgatgc gccagtcgcg cagcctggtc tcgccctccg gcggcgggcc
     2641 ctcccagtcg atccgcgggt cggcccaggc gtggccctca ccgggtgcga gctgcgccgc
     2701 gacgccgccg tcctcgtcgt agaggacgac cgtcctctcc gtccagttct gcaccgagag
     2761 ccgctcgtgg cctgccacca cgcggacgag caggccgggg agcggtgggt cgatcccgga
     2821 gaccgtcgag acgaagcccg tgtggatccc ggtcgtatgg gcgtgtgcgg cgcccgcagc
     2881 gacgagcgcg agcagcagag tggcgaggac gacgcgcatc gccgaatcct gccctgctca
     2941 gcgcacccag gcgagcgccc ggacggcgat cggccagcgg gccatggcga gcgtccccgc
     3001 caggtggtgg aggacgatct cctcgccctc cgcgaccgcg agccactcgc cgtccggcga
     3061 ccacgcggcg gccctgccct cgaacgtccc ctgcacgccc gcgcagacgg agccgaggac
     3121 gacggaggcg ggggcgacgg ggaaggcgag gtcgcacccg tccgcggaga gcgcgggcgg
     3181 gccggtcgtc gccggcgcgc ggacgcggcc gagaggctcc ccgccgcgat accggccgat
     3241 cacgccgtcg gcgaggaaga gggtgacggt gtcgtcgcgg ttgaaggtgg cgcgcacgat
     3301 ccgctcgggc gcctcgagca cgggctcgcc gccgacgagg aggcggccgc cgtccgcgaa
     3361 cgcgggccgc tcgcccggcg cgtagccgac cgggcagccg cggagcgcgc gcggccgctc
     3421 cgccgtgcgg accggcaggt cgtagccgcg gccggggtcg tggcaccaga acgcgtgctg
     3481 gccgtccgcg ctccacgtga cgccgttgaa cgtctggtac gtcccgagcg gctcgtccgg
     3541 gtggctgagg tcgaggagcc ggaacggccg gaaggcgccg gcccgccacg ggccctcgag
     3601 cgcgtaggcg acgagcccgc cgcgtggcgc cgcccagagc tcgcagccgg tctcgatccg
     3661 cgggagcggg agctcctggc cgcccgagac gacgatctgc cggagcgtgc acccgtcgtc
     3721 gtcgaggagg acgagcgagc ccggcgccgg cacgccggcg agcctgcccg agccgtcgtc
     3781 gaccgtggcc gtctccgtcg agccggtcgc cgcggcgggc tcgacggcgt cctcgccgcc
     3841 ccgcacggcg tccgcgagcg cgacgccggc cagcacgagc accccggcga cgatcagcgc
     3901 gtcccggaac ctcccgtacg aagcgggatt tcgcggcacc gtggctataa tgcccgcttg
     3961 ctcatgaccg agaggaccgt gacggaagca gatccgaccg agtacgacgc ggacgtctgg
     4021 acacgtggtc cggacgggga gctcgtcccc aactacgacg cgacgttccc cacgatcaac
     4081 gagggggagg tcgtcagcgg caaggtcgtc cgcgttgaca aggacgaggt gctcgtcgac
     4141 atcggctaca agtcggaggg cgtcatcccg gtcgcggagc tctcgatccg ccgctcggtc
     4201 gacccggccg acgaggtgca gctcggggac gacgtcgacg cgctcgtcct cacgaaggag
     4261 gatgccgagg gccggctgat cctctcgaag aagcgcgccc ggttcgagat ggcgtggaag
     4321 cggatcgagg gcgcggccga gagcggcgag cccgtcgagg gcgtcgtgat cgaggtcgtc
     4381 aagggcgggc tcatcctcga cctcggcgtg cgcggcttcc tgccggcgtc gctcgtcgac
     4441 atccgccgcg tgcaggatct cgaggacttc ctcgggcaga cgctccgctg caaggtgatc
     4501 gagctgaacc gctcgcggaa caacgtcgtc ctctcccgcc gcgccgtgct cgaggaggag
     4561 cgcaaggagg cgcgccaggc gatcctcgac cggctctcgc cggggaacgt cgtgcagggg
     4621 acgatctcga acatcgtcga cttcggcgcg ttcgtcgacc tcgacgggat cgacggcctc
     4681 atccacatct ccgagctctc gtggagccac gtgaaccacc cctccgaggt gctcgacatc
     4741 ggccaggagg tcgaggtcaa ggtgctcgac atcgaccgcg accggcagcg gatctcgctc
     4801 ggcctcaagc agacgcagag cgacccgtgg cagcaggtcg tcgagacgca cggcgagggc
     4861 gacgtggtcg aggggcgcgt gacgaaggtg gtcacgttcg gcgccttcgt cgagatcctc
     4921 ccgggcgtcg aggggctcgt gcacatctcc gagctcgcga accaccacgt cgagaacccc
     4981 cgcgagatcg tgaaccaggg cgaggacgtc cgcgccaaga tcatcgagat ggacgccgac
     5041 cggcgccgcc tctcgctctc gctcaagcgg gtcgagggcg acggcgccgc cgccgacccg
     5101 tccgagctcg gcctctccga ggaggtcttc acggaggacg aggcagcgct cgaggacgag
     5161 ccggcggccg aggccgaggt cgagccggag ctcgaggccg aggtcgagcc ggaggtcgag
     5221 gcggccgagg agcccgaggc ggaagcggcc gccgagcccg aggccgaggc cgaggcgggc
     5281 gccgagccgg ccgagcccgc cgaggacgag caggcctagg agccgcgtcc ggcgcccgtc
     5341 gccggtggtt ccccggtgtt ccgcgcccgg aaagagcaga gcaccgcacg tctcggcgcc
     5401 ggcccgcgtc ccgtcgcact cgccgtgacc ggctcgatcg gcgccggcaa aagcgaggtg
     5461 ctggccgcgt tcgagcgtca cggcgcggcg gtcatctcga gcgacgacat cgtccaccgg
     5521 ctcctgcgcg aggacccggc ggtgaagggc gcgatcgtcg agcggctcgg ggagcgggtg
     5581 ctcggcgagg accgcgagat cgtgcgctcc cgcgtcgcgg agctcgtctt ctccgatgcg
     5641 gaggcgctcc gctggctcga ggagctgctc cacccgcgcg tcgtcacgac gtacctgcgc
     5701 tggcgcgagg acctgcgcgc gggcgccgat ccgcccgacg tctgcgtgac cgaggtgccg
     5761 ctcctctacg aggtcggggc cgaggagcgc ttcgacgccg tcgtcgcggt ctccgcctcg
     5821 ccggaggtgc gggcggcgcg gctcaggcac cccgtgtccg accgcgagac acggcttctg
     5881 ccggacgcgg agaagctcgc ccgcgccgac ttcgcgtacg tgaacgaggg gtcgctcgaa
     5941 gagctcgacc gcttcgtgtc cgacgtgatg ggcaccctga cccgttcgtg agatacgccg
     6001 tcgcaaccct cctcgcggtc gccgggctcg cggccgcggc ggtcgcgctc gagcgctcgc
     6061 agccggc
//
LOCUS       DASTFC010000238         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_730550,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000238 DASTFC010000000
VERSION     DASTFC010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730550"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFO88_07055"
     CDS             <1..282
                     /locus_tag="VFO88_07055"
                     /inference="COORDINATES: protein motif:HMM:NF025923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545318.1"
                     /translation="STLVSGAKALLEAGATAVYACATHGLFPPDALASLGESELTEIV
                     VTDSVPQDVASAPDNFQILTVSGLLADTIQNVFSGESVSAIFAGENQLF"
     gene            complement(466..849)
                     /locus_tag="VFO88_07060"
     CDS             complement(466..849)
                     /locus_tag="VFO88_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545319.1"
                     /translation="MAFCPGGVENRGGNMAAVDTERARAGMLERSGGGRVVARLSTET
                     KHAFKTTEFWTMIAVVAGILIASALVGQGEGTGSEASDAFSASRAWLLVAIVAVGYMV
                     SRGLAKAGSREPYWADQDDLPGNKG"
     gene            1166..1357
                     /locus_tag="VFO88_07065"
     CDS             1166..1357
                     /locus_tag="VFO88_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545320.1"
                     /translation="MARTDRQEVALLRILIATGERALEAFQASENPVDADFVAELERI
                     IARSRDELAALTEHMTEST"
     gene            1464..1847
                     /locus_tag="VFO88_07070"
     CDS             1464..1847
                     /locus_tag="VFO88_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545321.1"
                     /translation="MTTLNDQRKAPALYALLMGVFVGGFAFVDATPPAFLAFTALLVA
                     GAAQIGVGFVIGRWGALALGVVPILFAVAAAGPGSTLWTTVFVLMAFPGTPLIGAGVH
                     LRQRFDERHDDSPDAWLYGEEPGNL"
     gene            complement(1902..2915)
                     /locus_tag="VFO88_07075"
     CDS             complement(1902..2915)
                     /locus_tag="VFO88_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545322.1"
                     /translation="MPDAADIKRGAAKAPSTRRKGAPKGAAVEVTAADPPVVRLEIYA
                     AQTPAPKAKRKARKTPAKPTAAPATTALPELERREAEVAEREETLARQAAEHEARGQA
                     LAAAADELAAREQELASRIEDFAARYDAVTRAAETFAEREAALAAREQAIGAEEARLA
                     ALRESTEAIETTAGERERVLAGRELELAGRERNLAEREQALDARAAELDRKEPALAER
                     ERVIALRERERAEQALTRAGDHGVAAERLELQRREKRLVQLEESLREKAQLLDERQSD
                     LELDRVQHETEADYRESKLEERERALEELERRLGAKESELTAYVAQVQGELRRRELEG
                     TSA"
     gene            3222..3929
                     /locus_tag="VFO88_07080"
     CDS             3222..3929
                     /locus_tag="VFO88_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9545323.1"
                     /translation="MAGDRIKLEVQAREARGSAEVRRLRAEGLIPGVLYGAGKEPQSF
                     CVGERELRRAITGDHGTHTILDVVVEGDGGKPRPAVLKDYQVHPTKSRLLHVDLHEVR
                     LDRPIQTAVGVELLGESEGVKVGGVLTQVTREVNVEALPMSIPDRLELDISALEIGDS
                     LRVADLVVPEDVTVLDDEDVVLASVAAPRVEEEPVVAEEEEGAELAEGEEAPEGEAEE
                     AAEPEADAAGEQETTEG"
BASE COUNT          506 a         1513 c         1464 g          587 t
ORIGIN      
        1 tcgacgctcg tctccggcgc gaaggcgctg ctcgaggccg gcgcgaccgc cgtgtacgcg
       61 tgcgcgacgc acggcctctt tccgcccgac gcgctcgcga gcctcggcga gagcgagctg
      121 acggagatcg tcgtcaccga cagcgtcccc caggacgtgg cctcggcgcc ggacaacttc
      181 cagatcctca ccgtttccgg cctcctcgcg gacacgatcc agaacgtgtt ctcgggcgag
      241 tccgtctcgg cgatcttcgc cggcgagaac cagctcttct agcctcgcag ctccgccgcc
      301 gcctcctcga tcgtcgtgac gccgcgaccg ggcctgcatc ctcatgcacc ggcgagcaga
      361 gatccgccag ccggcgcacc acggtaccct cgcgcggcct tggtacgggc aaaggatcca
      421 gcagggcggc cggttcaggc cggccgccct gcctgtctgc ggctactagc ccttgttgcc
      481 cgggagatcg tcctgatcgg cccagtacgg ctcgcggctt cctgccttcg ccagaccgcg
      541 gctgaccatg tagccgacgg cgacgatcgc gacaaggagc cacgcccgcg acgccgagaa
      601 ggcgtcgctt gcttcgctgc cggtgccctc gccctggccg acgagcgcgg aggcgatcaa
      661 gatgcccgcg accacggcga tcatcgtcca gaactccgtc gtcttgaagg catgcttcgt
      721 ctcggtcgac aggcgcgcaa caacgcgtcc gccgccgctt cgctcgagca ttcccgcccg
      781 agcgcgctcg gtatctactg ctgccatgtt cccccctcgg ttctccaccc caccggggca
      841 gaacgccact ctcgccagcg cctcagggcc ctaaacacct cgcctagggg cgggcgagcc
      901 gcgacccgat ccgggcgccc acgacctgcg tcccgcgagc ctcacgaacg gcgccgccgc
      961 cgtccccgat aggttgcgcc tacgccagga acgaccgcga gatcgggagc cgtccccgca
     1021 gcgcgggccg cttggcgcgt tcgagacccc gttcgacccc gttccccccg gcggggccga
     1081 acgggggctg atctggagcg agctcgcgca gccttccggc ggccccgcgc gtttggctgg
     1141 ccgctccacg ggctagaggc ccaccatggc ccgcacggac agacaggagg tcgcgctgct
     1201 cagaatcctg atcgcgacgg gcgagcgggc gctcgaggcg ttccaggcga gcgaaaaccc
     1261 cgtggacgcc gacttcgtcg cggagctcga gcgcatcatc gcccggtcgc gcgacgagct
     1321 ggccgcgctc accgagcaca tgaccgagtc gacctagcga cgcgcgggac ccccgagcgg
     1381 gggatggcgg gctccggccg gtcgcgcgat cattcggtca aggaccatgg cggggcggcg
     1441 cgccggagac cgtttggagg cgcgtgacta cgctcaacga ccagcgcaag gcaccggcgc
     1501 tctacgcgct cttgatgggc gtcttcgtgg gcggcttcgc cttcgtcgac gcgacgccgc
     1561 cggccttcct cgcgttcacg gcgctcctcg tcgcgggagc ggcgcagatc ggcgtcggct
     1621 tcgtgatcgg ccgctggggc gcgctcgcgc tcggcgtcgt gccgatcctc ttcgccgtcg
     1681 cggcggccgg gccggggtcg acgctctgga cgaccgtgtt cgtgctgatg gccttccccg
     1741 ggacgccgct catcggggcg ggcgtccacc tgcgtcagcg gttcgacgag cggcacgacg
     1801 actcgcccga cgcctggctg tacggcgaag agccgggcaa cctctaggga cgcaggacgc
     1861 cgagccggcg cgcgggcgcc gggcgccggg cggccgtgag gctaggcgct cgtgccttcg
     1921 agctcgcggc ggcggagctc gccctgcacc tgcgcgacgt acgcggtgag ctcgctctcc
     1981 ttcgccccga ggcggcgctc gagctcctcg agggcgcgct cgcgctcctc gagcttcgac
     2041 tcgcggtagt ccgcctcggt ctcgtgctgg acgcggtcga gctcgaggtc ggactggcgc
     2101 tcgtccagga gctgcgcctt ctcgcggagg gactcctcga gctggacgag ccgcttctcg
     2161 cgccgctgga gctcgagccg ctcggccgcg acgccgtggt cgccggcgcg ggtgagcgcc
     2221 tgctcggcgc gctcgcgctc gcggagggcg atcacgcgct cccgctcggc gagcgccggc
     2281 tccttgcggt cgagctcggc cgcgcgcgcg tcgagcgcct gctcgcgctc ggcaagattt
     2341 cgttcccggc cggcgagctc cagctcgcgt ccggcgagga cccgctcgcg ctcgcctgcc
     2401 gtcgtctcga tcgcctcggt gctctcgcgg agggcggcga gccgggcctc ctcggcgccg
     2461 atcgcctgct cgcgcgccgc gagcgccgcc tcgcgctcgg cgaacgtctc ggccgcgcgg
     2521 gtgacggcgt cgtaccgtgc ggcgaagtcc tcgatcctgc tcgcgagctc ctgctcccgg
     2581 gccgcgagct cgtccgcggc tgcggcgagc gcctgcccgc gggcctcgtg ctccgcggcc
     2641 tgccgggcga gcgtctcctc gcgctcggcg acctccgcct cgcgccgctc gagctcgggc
     2701 agcgcggtcg tcgccggagc cgccgtcggc ttcgcgggcg tcttccgggc cttgcgcttc
     2761 gccttcgggg cgggagtctg cgccgcgtag atctcgagcc ggacgacggg gggatcggcc
     2821 gccgtcacct cgaccgcggc gcccttcgga gcgcccttgc ggcgggtgga gggagccttc
     2881 gcggccccac gcttgatgtc tgcggcgtcc ggcatgctcg aggtgcggcc ctcgaacgaa
     2941 gcttcggcgc cggagggagg cggtgctatc cctcgcacga gcggttgcgc gcgacgagat
     3001 ctcgggtcac agtgagagca ccgggacggg actcggcgcc gtcccccgat gcggtactgc
     3061 ggagagccat ggaccccaac acccacgcct ttgccatggg cacggcgttc tttgcctcga
     3121 tcttcttcgc cctcgcggcc tggctgccgg tgggcatctt cttccggaac ctgttcttcg
     3181 gctactagag aagaccaggg ctccggtaca ctggccgacc gatggcgggc gaccggatca
     3241 agctcgaggt acaggcacgc gaggctcgcg gaagcgccga ggtgcggcgc ctgcgtgccg
     3301 aggggctcat tcccggcgtg ctctacggcg cgggcaagga gccgcagagc ttctgcgtcg
     3361 gcgagcgtga gctccgccgc gcgatcacgg gcgaccacgg cacgcacacg atcctcgacg
     3421 tcgtcgtcga gggcgacggc ggcaagccgc gcccggccgt gctgaaggac taccaggtgc
     3481 atccgaccaa gagccgcctc ctgcacgtgg atctccacga ggtgcggctc gaccggccga
     3541 tccagacggc cgtcggcgtc gagctcctcg gcgagtcgga gggcgtgaag gtcggcggcg
     3601 tcctcacgca ggtcacgcgc gaggtgaacg tcgaggcgct cccgatgagc atccccgacc
     3661 ggctcgagct cgacatcagc gcgctcgaga tcggggacag cctccgcgtc gccgacctcg
     3721 tcgtgccgga ggacgtgacc gtcctcgacg acgaggacgt cgtgctcgcc tccgtcgccg
     3781 cgccgcgcgt cgaggaggag ccggtcgtgg ccgaggagga ggagggcgcc gagctcgccg
     3841 agggcgagga ggcccccgag ggcgaggccg aggaagcagc ggagccggag gcggacgccg
     3901 ccggcgagca ggaaacgacc gaaggctagg cgcctgtgcg gttgttccgt cggggcgagc
     3961 gggcctcgac gctcgacctg ctcgtggcgg ggctcgggaa ccccgggcgc gagtacgcgg
     4021 cgacgcgcca caacgtcggc ttcctcgtcg cggacgagct cgcgcgccgg
//
LOCUS       DASTFC010000239         5523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_318698,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000239 DASTFC010000000
VERSION     DASTFC010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318698"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFO88_07085"
     CDS             complement(<1..665)
                     /locus_tag="VFO88_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9545324.1"
                     /translation="MKIGDTIADRYRLDGVLGSGGMSRVYRAHDNVLERDVALKVLHD
                     KLSDDPSYVERFRREARAIARLSHPNIVTVIDRGQVDSAEFIVFELVRGANLKQLLSE
                     RRYLPVREALAIVHQAARGLAFAHEHGIVHRDVKPQNVLVDDDGAAKVTDFGIARSLD
                     RESGLTQTGAILGTADYLSPEQATGQPVTEAADQYSLGVLLYELLTGEVPYRGESVVA
                     VAAR"
     gene            complement(704..2029)
                     /gene="gabT"
                     /locus_tag="VFO88_07090"
     CDS             complement(704..2029)
                     /gene="gabT"
                     /locus_tag="VFO88_07090"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HET9545325.1"
                     /translation="MATTTSIQRKTEIPGPISKAILERHERVIAEPLSIYLPLVIAEG
                     RGALLTDVDGNTFVDFTGGIGCLAVGHSHPRVVEAAQEQTARFTHTDYTIVPYENYVA
                     LAERLTEAAPISGEVRAGFFNAGAEAVENAVKFSRSYTKRPAVIAFDGAFHGRTLMAM
                     SLTSKTHPYKAGLGPFAPEVYRVSYADPYRGPDTETALAELRRAFKTRVAAEEVAAII
                     LEPVQGESGFVVPPKEFLQGVREIASEHGIVLIVDEVQTGFGRTGTMFAIEQFGIEPD
                     LMTIAKSVAAGFPLSGVVGRAEIMDSPADSAIGGTYVGNPVCIAAAHATLDVIEEEGL
                     VERANAIGETIREHMEGWRERFSCVGDVRGLGAMLAIEIVSDREAKTPDADLATRIVE
                     EAASRGLLLLKAGIYGNCIRVLVPLVVTDEQLSEALGAWDEAFSAAGAR"
     gene            2085..2660
                     /gene="dcd"
                     /locus_tag="VFO88_07095"
     CDS             2085..2660
                     /gene="dcd"
                     /locus_tag="VFO88_07095"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET9545326.1"
                     /translation="MVLSDRTIRRLLEEGRIGIDPYDEELLQPSSVDVRVDRFFRVFR
                     NSRYPFIDVKEPMEELTELVEIEGDEPFILHPGEFVLGSTLERITLPDDLVARLEGKS
                     SLGRLGLLIHSTAGFVDPGWDGHVTLELSNVANLPITIYHAMKIGQLSFVQLTEPAET
                     PYGSSALGSKYQGQQGPTPSRYWQNFAVTRR"
     gene            2657..3544
                     /locus_tag="VFO88_07100"
     CDS             2657..3544
                     /locus_tag="VFO88_07100"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET9545327.1"
                     /translation="MKVLVTGGTGFVGPHVVHAIRAQGHDVRALARRPGSKAARTLES
                     WGCELAEGSMTDADSLRRAVEGCDAVVHLVALLFGTPEAFRRVMEQGTRDLVAAAKEA
                     GVERFVLMSANGAVEGRELTPYFAAKWDEEQAVAESGIPYVTFRPSFVFGPDGGQLPI
                     QLRLARFSPVTPLMSRRRFQPIWVDDVAAFFAAALTNEEATNRVFGLGGPDVVTWEEL
                     HAEIRRVLGKRRLTLRVPAGLLKASASIGQVLPPFHGARAAVEMLDFDDNVADPGPAI
                     EVFGIRPIDLRSQIRRAVS"
     gene            complement(3784..4371)
                     /locus_tag="VFO88_07105"
     CDS             complement(3784..4371)
                     /locus_tag="VFO88_07105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9545328.1"
                     /translation="MLDERVLEVLARLEREDEAEGERDLPMQERSLAVEPTTGRFLFS
                     LVAPQTACEVLELGGSRGYSTIWLGAGVRYLGGRVLSVEQDPAKAEAWRRNVADAGLA
                     DWCELVEADAFGVLDGLEDIFDVVFLDAWKGDYEALFALARRRLEPGGLVVADNVLSH
                     VEELGAYSEARQSDPTLTSVTVPLDRGLELSVVLR"
     gene            complement(4371..4688)
                     /locus_tag="VFO88_07110"
     CDS             complement(4371..4688)
                     /locus_tag="VFO88_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545329.1"
                     /translation="MSREARVAKNEVLFREVNERIRELAPGDGPTEFLCECGDAGCVE
                     SVSLSTGQYEEVRRDPARFFVRPGHEVPDIEDVVEEAGSFLVVAKRQGTPAEIAEEND
                     PRS"
     gene            complement(4712..5023)
                     /locus_tag="VFO88_07115"
     CDS             complement(4712..5023)
                     /locus_tag="VFO88_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET9545330.1"
                     /translation="MDYEVCPVDELPPGTMKLVPAGSISVGVYNVNGTLCALEDRCSH
                     DDGPLVEGDWEPDRGVVICPRHGATFDICSGKALTLPAYLPVDTFPVRVEDGVVIVDV
                     A"
     gene            complement(5027..5461)
                     /locus_tag="VFO88_07120"
     CDS             complement(5027..5461)
                     /locus_tag="VFO88_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9545331.1"
                     /translation="MNEFDAMYREVILDHYKNPRGHGEIEDADAHAEGQNPLCGDEVA
                     IYVEFGPDGETIEDVKFRGRGCAISQAATSMLTEMVRGRKAADVASLPRDELLDEVGI
                     PLTPIRLKCALLGLGVLKVALHKAKGTPLPEEWAGVDELQIN"
BASE COUNT          789 a         1992 c         1923 g          819 t
ORIGIN      
        1 cgcgccgcga ccgcgacgac gctctccccc cggtacggca cctcgccggt cagaagctcg
       61 tagagcagga cgccgagcga gtactggtcg gccgcctccg tgacgggctg ccccgtcgcc
      121 tgctccggcg agaggtagtc ggcggtgccg aggatcgcgc ccgtctgcgt gagcccggac
      181 tcgcggtcga gcgagcgcgc gatcccgaaa tcggtcacct tcgccgcgcc gtcgtcgtcc
      241 acgaggacgt tctgcggctt cacgtcgcgg tgcacgatcc cgtgctcgtg ggcgaacgcg
      301 aggccgcgcg ccgcctggtg gacgatcgcg agcgcctcgc ggacgggcag gtacctccgc
      361 tccgagagga gctgcttcag gttcgccccg cggacgagct cgaagacgat gaactcggcc
      421 gagtcgacct ggccgcggtc gatcacggtg acgatgttcg ggtgcgagag ccgcgcgatc
      481 gcgcgcgcct cccggcggaa gcgctcgacg tacgaggggt cgtccgaaag cttgtcgtgc
      541 agcaccttga gcgcgacgtc gcgctcgagc acgttgtcgt gcgcgcgata gacgcgtgac
      601 atgccgccgc tgccgagaac gccgtcgagc ctgtagcggt cggcgatcgt gtcgccgatc
      661 ttcaccttga cagtctgccg cgcgcgggca cgcggcgaac cctctaccgc gcgcccgccg
      721 cggagaacgc ctcgtcccag gccccgagcg cctcgctcag ctgctcgtcc gtcacgacga
      781 gcggcacgag cacccggatg cagttcccgt agatcccggc cttgagcagg agcagcccgc
      841 gcgaggcggc ctcctcgacg atccgggtgg cgaggtcggc gtcgggcgtc ttcgcctcac
      901 ggtcggagac gatctcgatc gcgagcatcg cgccgagtcc gcgcacgtcg ccgacgcagg
      961 agaagcgctc gcgccagccc tccatgtgct cccggatcgt ctcgccgatc gcgttcgcgc
     1021 gctcgacgag cccttcctcc tcgatcacgt cgagcgtcgc gtgggcggcc gcgatgcaca
     1081 cggggttgcc gacgtacgtg cccccgatcg cggagtcggc cggcgagtcc atgatctccg
     1141 cccgcccgac gacgcccgag agcgggaagc ccgccgccac cgacttcgcg atcgtcatca
     1201 ggtcgggctc gatcccgaac tgctcgatcg cgaacatcgt cccggtgcgc ccgaagcccg
     1261 tctgcacctc gtcgacgatg aggacgatcc cgtgctccga ggcgatctcg cgcacgccct
     1321 ggagaaactc cttcggcggc acgacgaagc cgctctcgcc ctgtaccggc tcgaggatga
     1381 tcgcggcgac ctcctcggcc gcgacgcggg tcttgaacgc gcggcggagc tccgcgagcg
     1441 cggtctcggt gtccggtccg cgatacgggt cggcgtacga gacccggtag acctccggtg
     1501 cgaacggccc gaggcccgcc ttgtacgggt gcgtcttcga cgtgaggctc atcgccatga
     1561 gcgtgcggcc gtggaacgcg ccgtcgaagg cgatgacggc ggggcgcttc gtgtacgagc
     1621 gcgagaactt gaccgcgttc tcgaccgcct cggcgccggc gttgaagaag cccgcgcgca
     1681 cctcccccga gatcggcgcg gcctcggtga gccgctccgc gagggcgacg tagttctcgt
     1741 acgggacgat cgtgtagtcg gtgtgcgtga agcgcgccgt ctgctcctgc gcggcctcga
     1801 ccacccgcgg gtgcgagtgg ccgaccgcga ggcagccgat gccgccggtg aagtcgacga
     1861 acgtgttccc gtccacgtcg gtgaggagcg cgccgcgccc ctccgcgatg acgagcggga
     1921 ggtagatcga gagcggctcc gcgatgacgc gctcgtgccg ctcgaggatg gccttcgaga
     1981 tcgggcccgg aatctcggtc ttgcgctgga tggaggtcgt ggtcgccatg gcgcgcatcg
     2041 tatgcggtcc gggcgctgcc ccgccccacc tagaatgcgc cgcgatggtc ctctccgacc
     2101 gcaccatccg ccgcctgctc gaggagggga gaatcgggat cgacccctac gacgaggagc
     2161 tcctccagcc gtccagcgtg gacgtgcgcg tcgaccgctt cttccgcgtc tttcgcaact
     2221 cgcgctaccc gttcatcgac gtgaaggagc cgatggagga gctcacggag ctcgtcgaga
     2281 tcgagggcga cgagccgttc atcctccatc ccggcgagtt cgtcctcggc tcgacgctcg
     2341 agcggatcac gctgccggac gacctcgtcg cacggctgga agggaagtcg agcctgggga
     2401 ggctcggcct gctcatccac tcgaccgccg ggttcgtcga ccccggctgg gacgggcacg
     2461 tgacgctcga gctctcgaat gtggccaacc tcccgatcac gatctaccac gcgatgaaga
     2521 tcgggcagct ctcgttcgtg cagctgacgg agccggccga gaccccgtac ggatcctcgg
     2581 cgctcggctc gaagtaccag ggccagcagg ggccgacgcc gagccgctac tggcagaact
     2641 tcgcggtgac ccggcgatga aggtcctcgt caccggcggg accgggttcg tcgggccgca
     2701 cgtcgtccac gcgatccgcg cgcagggcca cgacgtccgc gccctcgcgc gcaggccggg
     2761 ctcgaaggcg gcgcgcacgc tcgagagctg gggctgcgag ctcgccgagg gctcgatgac
     2821 cgacgctgac agcctgcggc gcgcggtcga gggctgcgac gctgtcgtcc atctcgtcgc
     2881 gctcctcttc ggcacgccgg aggcgttccg ccgggtgatg gagcagggca cccgcgacct
     2941 cgtcgcggcg gcgaaggagg cgggcgtcga gcgcttcgtc ctcatgagcg cgaacggcgc
     3001 cgtcgagggg cgggagctca cgccgtactt cgcggccaag tgggacgagg agcaggccgt
     3061 cgcggagagc ggcatcccgt acgtgacctt ccggccgagc ttcgtcttcg gccccgacgg
     3121 cggccagctc ccgatccagc tccgcctcgc gcgcttctcg ccggtcaccc cgctcatgag
     3181 ccgccggcgc ttccagccga tctgggtgga cgacgtggcc gccttcttcg cggccgcgct
     3241 cacgaacgag gaggcgacga accgcgtgtt cggcctcggc gggccggacg tcgtcacgtg
     3301 ggaggagctg cacgccgaga tccggcgcgt cctcggcaag cggcggctca cgctccgcgt
     3361 cccggccggg ctgctcaagg cgtccgcgag catcgggcag gtgctcccgc cgttccacgg
     3421 cgctcgggcc gcggtcgaga tgctcgactt cgacgacaac gtcgccgacc ccggcccggc
     3481 gatcgaggtc ttcggcatcc gcccgatcga cctgcgctcc cagatccggc gagcggtctc
     3541 ctagcgcgag ttcccagaaa cgcagcgggg ccccgaaggg ccccgcgcga atcggtgact
     3601 cgcctggatt tcgtaggagc gctcggcccc ctacgctggc taggcgattc cccttcggtc
     3661 tcggggacac gcccctacga gcgagtacct ccactgcccg tactgtcaga aatcaacgtc
     3721 ggaccacctc ctttccgtgg tggtcgcatg atacgcacac tcggtggacg tccggaaggg
     3781 gagtcagcgg aggacgaccg agagctcgag gccgcggtcg agggggacgg tgacgctcgt
     3841 gagcgtcgga tcggactgcc gcgcctcgga gtacgcgccg agctcctcga cgtgcgacag
     3901 gacgttgtcc gcgacgacga gcccgcccgg ctcgagccgc cgccgcgcga gcgcgaagag
     3961 cgcctcgtag tcccccttcc acgcgtcgag gaagacgacg tcgaagatgt cctcgagccc
     4021 gtcgagcacg ccgaacgcgt cggcctcgac gagctcgcac cagtctgcga ggccggcgtc
     4081 ggcgacgttg cggcgccacg cctcggcctt cgcgggatcc tgctcgaccg agaggactcg
     4141 cccgcccaga taccgcacgc ccgcgccgag ccagatggtc gagtacccac gcgagccgcc
     4201 gagctcgagc acctcgcagg cggtctgcgg cgcgacgagc gagaagagga agcggccggt
     4261 ggtcggctcg accgcgagcg agcgctcctg catcgggaga tcgcgctccc cctccgcctc
     4321 gtcctcgcgc tcgagccggg cgagcacctc gagaacgcgc tcgtcgagca tcaggagcgc
     4381 gggtcgttct cttcggcgat ctcggcgggc gtaccctggc gcttggcgac gacgaggaag
     4441 ctgccggcct cctcgacgac gtcctcgatg tccggcacct cgtggccggg gcggacgaag
     4501 aaccgcgccg ggtcgcgccg cacctcctcg tactgaccgg tcgagagcga gaccgactcg
     4561 acgcagccgg cgtcgccgca ctcgcagagg aactcggtcg ggccgtcgcc cggcgcgagc
     4621 tcgcggatgc gctcgttcac ctcgcggaag agcacctcgt tcttagcgac gcgggcctcg
     4681 cgggacatcg cttgatcgta ctctgctcgg ctcaggcgac gtcaacgatc acgacgccgt
     4741 cctcgacccg gacggggaac gtgtccacgg gcaggtacgc cggcagcgtc agcgcctttc
     4801 ccgagcagat gtcgaacgtc gccccgtggc gcgggcagat gacgacgccg cggtcgggct
     4861 cccagtcgcc ctcgacgagc ggcccgtcgt cgtgcgagca gcggtcctcg agcgcgcaga
     4921 gcgttccgtt cacgttgtag acgccgacgg agatcgagcc ggccgggacg agcttcatcg
     4981 tccccggcgg cagctcgtcg acgggacaga cctcgtagtc caacggctag ttgatctgga
     5041 gctcgtcgac gccggcccac tcctcgggga gcggggtgcc cttcgccttg tgcagcgcga
     5101 ccttgaggac gccgagcccg agcagcgcgc acttgagccg gatcggcgtg agcgggatcc
     5161 cgacctcgtc gagcagctcg tcgcgcggga gggacgccac atcggccgcc ttccggcccc
     5221 tgaccatctc ggtcagcatc gacgtggccg cctggctgat cgcgcagccg cggccgcgga
     5281 acttcacgtc ttcgatcgtc tcgccgtcgg ggccgaactc gacgtagatc gccacctcgt
     5341 cgccgcagag cgggttctgc ccctcggcgt gcgcgtcggc gtcctcgatc tccccgtgcc
     5401 cgcgcgggtt cttgtagtgg tcgaggatga cctcgcggta catggcgtcg aactcgttca
     5461 cacggtcatc gtagccgcct ggatgcagcc cggaacccgt ccgccacgag cgtcgcgatg
     5521 ccg
//
LOCUS       DASTFC010000240        13570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_302359,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000240 DASTFC010000000
VERSION     DASTFC010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302359"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFO88_07125"
     CDS             complement(<1..977)
                     /locus_tag="VFO88_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9545332.1"
                     /translation="MLAGLAGGLALPAPAEATARPKPVVVKTERQFQQAVNRLKRPGG
                     TIVLRGGRYRMLRVGPRSARPLVVRAQRGVSVRRILLRETRSVRLENVVLLPDARPAS
                     LEVRRSRGVRLDGIRVRGTTVRRARVVLDASNGVTITASRFSRCGDGAVCLLLGRSSD
                     VLVEGSRFEDCFGCDFVRGRAGTGLVLRGNRFLRALVGPCGTDPAACNHNDLVQVQAG
                     TNVLVERNVFGLVEPPGAGQLYLSGPIDGLVVRDNLFRATDPALPGFPVPPNGIVLGN
                     RLGPDALPANVTIAHNTILSGARRGFGQYPDLANSLVVSPRYGWNPESQR"
     gene            1109..1918
                     /gene="proC"
                     /locus_tag="VFO88_07130"
     CDS             1109..1918
                     /gene="proC"
                     /locus_tag="VFO88_07130"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET9545333.1"
                     /translation="MNRQIAILGAGKIGESLISGLLSSEWRDPGEIVATSRRDDRLAE
                     LAERYGIRTTRSNAEAVEEAGIVVIAVKPQDIEGLLDELRGRLRPGQTIISIAAAIPT
                     SLIEGYLGDSVAVVRAMPNTPAAVHEGIAGIAAGANAGEDHLAAAEEVLSHLGRSVRV
                     DESYMDAVTAVSGSGPAYFALLAESMIEAGILLGLSREISTDLVVQTMLGTAKLLRDE
                     GMHPVELREMVTSPGGTTIAAIRELEQAGVRAAFLNAIQAAMRRSQELAAG"
     gene            1953..3317
                     /locus_tag="VFO88_07135"
     CDS             1953..3317
                     /locus_tag="VFO88_07135"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HET9545334.1"
                     /translation="MTEATRFLLGESDIPARWYNVQADLPEPLPPVIHPGTGGPIGPD
                     DLAPLFPMELIGQEVSQERWIDVPEEVREIYRLWRPTPYVRARRLEAALETPARIYYK
                     YEGVSPVGSHKANTAVPQAFYNKREGVGGLTTETGAGQWGSALAMACRFFDLPCEVFM
                     VRVSYEQKPYRRSLMQTFGATVTPSPSDETSAGRAVLADDPGSPGSLGIAISEAVEAA
                     ATSGGAKKYALGSVLNHVLLHQTVIGEETLLQLELAGDYPDLVVGCVGGGSNFGGFAL
                     PFVRENLAGRRSTRIVAVEPAAAPSLTKGRYVYDFGDTARTTPLIKMYTLGHSFVPAP
                     VHAGGLRYHGMAPIVCHLYDLGLIEARAVSQNPVFDAAVTFARAEGIVPAPEPAHAIR
                     VVIDEALACKESGEERTIAFNLCGHGHFDMGAYDEYLAERLPDFEYAEEDVERALAEL
                     PAVG"
     gene            complement(3314..4102)
                     /locus_tag="VFO88_07140"
     CDS             complement(3314..4102)
                     /locus_tag="VFO88_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HET9545335.1"
                     /translation="MGKPRVAALQIGLQAKGLYGGTIDGHYGPATEQAVRRLQRRAGL
                     SVDGVAGPQTRRALGRYGRHDFGSRVLRNGRVGWDVSVLQFRMAWRGFPSGALDGVFG
                     PRTTSAVIRFQRYARLLADGLPGPATYRALRRPLKRVPIALGRPVRGLLTDRFGPRGN
                     RFHTGLDFAAPAGRAVRAAAPGVVVSAGWDTGGYGRLVVVRHAHRVHTWYAHLRGFRV
                     RPGQAVASGERLGRVGATGRATGPHLHFEARRRGAALDPAPALR"
     gene            complement(4221..5234)
                     /locus_tag="VFO88_07145"
     CDS             complement(4221..5234)
                     /locus_tag="VFO88_07145"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET9545336.1"
                     /translation="MAGRLTLPGFVNAHSHAFQRALRGRVDGGDFWAWRDAMLDVARG
                     QSPERVRQDYVAVYREMLAAGFTAVGEFHYLGLDQAYAASDAAAEAGIRFVLLHVAYA
                     RGGIDELRQPTVDAYLQEVAALRRDGVAVGVAPHSVRACPPDWLEAVGAYAAEHELPL
                     HVHACEQPREIEECLAEHGLRPIELLARTGCLGPRTSVVHATHASDAELDLLAEHGAR
                     ICVCPTTEASLGDGFVPVGRIRARGIPLCVGSDSNVRIDPLEELRELEGIARRLELRR
                     GVLDVDELLRIGSVEGAAALGLDAWGDVTADLGHPSLAGVGPDEARAALVFGCGAEVL
                     TPV"
     gene            5305..5856
                     /locus_tag="VFO88_07150"
     CDS             5305..5856
                     /locus_tag="VFO88_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9545337.1"
                     /translation="MATRAASAPYAHIPNALTLARFALIPVFVAMVLAADGAGSYAAA
                     AVFALAGATDQVDGWLARRWHVESRFGKLADPLADRLMIDAAVVLMWLDGRLPLAALA
                     LILARDGALLLGYPLVRNRGYDFEVNFLGKLATWVLYAALVGVVASDEGTTWPLVLFW
                     TGVALAVAAAAQYLAKALREVRR"
     gene            5853..8357
                     /locus_tag="VFO88_07155"
     CDS             5853..8357
                     /locus_tag="VFO88_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9545338.1"
                     /translation="MKAVVMAGGEGTRLRPLTSNQPKPMVPIVGKPCMEHIVELLRRH
                     GFDDIVVTLAFMPQMIRGYFGNGERHGVDIRYSVEETPAGTAGSVKLAEEALDEPFLV
                     ISGDALCDIDLSALVRFHEEREALVTIALKSVDNPLEFGIVVTDEDGRIERFLEKPSW
                     AQVFTDTINTGIYVVQPEALRDVPTDRPYDFAKELFPRLLEKGRPLYGFVVDGYWQDI
                     GNLDQYRKANFDALDEAVDLEIPGIVLRGNVHVGEGVELDDVESVEGPAYIGNYCRIH
                     PDATIGPYTVLSSSVAIREQARIERSIVDSSTYIGRSAVVEGAIVGRMCDIREHARLH
                     EGSAIGDQTTIGDEAVVFPEIRVYPFKEIESGAQVDRNVIWEARATASIFDREHIRGL
                     PNVDLTPEIALRLGMALGTALKRDARVVTSREAAPACRLLRRAVLAGINATGVHVADL
                     RVLPAAVNRHLVKSEGYEAGVHVGTSASDPEAVEIQVYEPPGVQASQSFMKEIEKHFS
                     RQEFRRAAADEVGQLVFPARAAESYVEDLLATLDADAIRARGFRIVVDYGYSSASLVL
                     PLVVGSLGVEIVASSPYVRDQVAIPQAATIRESLNRASRLVGAVAADLGVVLDPGAER
                     IYLIDEGGRAVPPDQELLLFERLIGSNGTTGRVAFPVTVTSLVDRIAEGTGLEVVRTP
                     ASLAALTRAAAHDGVVFAGSASGGFVFPDFVPGYDAITSLCKLLELLAPLGRPLSEVV
                     DEVPRPLVAHRAIRCPWSRKGAVMRVLTEQTKDRETDTLDGLKVFIDGGWAQVLPDPA
                     EPVVHVYAEGTSEDETHRLEEEFTTLVESIIQDDDA"
     gene            8436..8696
                     /locus_tag="VFO88_07160"
     CDS             8436..8696
                     /locus_tag="VFO88_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545339.1"
                     /translation="MEPLPDLNSLSDSELRDLIAKLVEEENEVSYRRRILHGKIDILK
                     AELQARLQRQVGEEGQSPLYEIDVDKLADILSARAAPPNPAD"
     gene            8706..9179
                     /locus_tag="VFO88_07165"
     CDS             8706..9179
                     /locus_tag="VFO88_07165"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9545340.1"
                     /translation="MTHIHCPECGFQNPESANYCSKCGALLIREEPGGHTTMSFAPEE
                     LADDESGMALEDLPVEGTALVVRAGGGRQGETFPLDQGRHEIGRSPDAEIFLDDVTVS
                     RSHAIVTRDESGVVIEDLGSLNGTYVNRRRVDKATLEDGDEVQIGKYRLTLLSRS"
     gene            9179..9874
                     /locus_tag="VFO88_07170"
     CDS             9179..9874
                     /locus_tag="VFO88_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9545341.1"
                     /translation="MATATREKSGRRLTIGAVRDRLADEFPDISISKIRYLEGRGLLA
                     PRRTQSGYRLYSEDDVERLRTILRLQRDEFLPLKVIREELASPGAKRRHQRKREADGG
                     MLAAAEPEIDLRTLCERAAVEPSFVRELEEYGLLEPRREDGEALYPERDAEVVAACAR
                     LARFGIEPRNLRSIRNGASSAAGLLEQIVAPALRSQNPERRLAGLEDLETLAAASQEL
                     SQLLFWRALRSLV"
     gene            9896..10414
                     /locus_tag="VFO88_07175"
     CDS             9896..10414
                     /locus_tag="VFO88_07175"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002633.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9545342.1"
                     /translation="MTVDLEAKIRDIPDFPKQGIVFKDIMPLLADAETFHATIDLLAE
                     FGEPRKPDLVLGAEARGFMLGGALAYKLGCGFVAARKPGKLPWETVSAEYALEYGVDS
                     LELHADAIRAGARVLVHDDLLATGGTARAKVELVEQLGGEVVGLAFVIELAFLNGREK
                     LEGYDVHSLIQY"
     gene            10457..11053
                     /locus_tag="VFO88_07180"
     CDS             10457..11053
                     /locus_tag="VFO88_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545343.1"
                     /translation="MYDGLTGRYTFRCPATGEARVRLSRFRSVERLAGPARPSVFRVR
                     FACPCGDDHEGLVTHDELDWAPLGSDASAFFNVMTRRLEPARDELLELAARRIGAGEW
                     PWTFFCWPEGRPRPVFPSAFRLLAEAEERLGVAVRCPDCGGTSVNVVSRRHVDEPFFN
                     DDAVAVVEHVFAADAEELVAGFRAELDSAAFDARRLAL"
     gene            11137..11856
                     /locus_tag="VFO88_07185"
     CDS             11137..11856
                     /locus_tag="VFO88_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545344.1"
                     /translation="MALLQRRTDERETTTVDDDVATERAGASPGIVRALFTLAGVAGA
                     GLLLWLAQLFNLDEASGFWVAAGIVAAAGLALGLSQLFGGWTKWGWPTMSPMMFLLAF
                     IPTLVVGGILLLAWQPDGGEGQEFANDVISTLGVGGLVDALEPFMWVVPLVIGLVFSF
                     TFDTTGPRTRIVDTDGTVLRERVVTHDREAVPDEDVHDYRGDDDRRDDGSVAEELRER
                     DRLQATRTTTDGDTRVDRDRI"
     gene            11958..12161
                     /locus_tag="VFO88_07190"
     CDS             11958..12161
                     /locus_tag="VFO88_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545345.1"
                     /translation="MDAASTFVPETGTGMTRILGDCEQLGRVTGDRSSSALRRLETVI
                     GEELAGRLVGALAGGRRSRGFPF"
     gene            complement(12167..12358)
                     /locus_tag="VFO88_07195"
     CDS             complement(12167..12358)
                     /locus_tag="VFO88_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545346.1"
                     /translation="MHLRPPVFSHGFTSFLWAFGLGAYVWLGLVAVGVGVGTALLLGL
                     LAGLGIFFLVLFYGGEEFR"
     gene            complement(12693..>13570)
                     /locus_tag="VFO88_07200"
     CDS             complement(12693..>13570)
                     /locus_tag="VFO88_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HET9545347.1"
                     /translation="DEGMTALQIDVKDENGEVAFASPLAPLATRVGAARDYYEPERVA
                     ERVHAKGLYLIGRLVVFEDPYLARERPGLAVQSSAGGIWTNYGGLGWSNQYDERVWDY
                     NVDIAEAAARAGFDEIMFDYVRFPSDGPIEQARWPGRVNEKKGETIARFLAYARDRLE
                     PLGVRISAAVFGLAATRDLGIGQRPRLLAPHLDVIYPMVYPSHYSPGEYDLPDPDGTP
                     GATVTMALRDFKEVMRGADTLIVPWLQDFTMGRQYGLEQIQAQVTAARKLTKAGYLLW
                     NAAGVYTPGGLAPAP"
BASE COUNT         1908 a         4907 c         4933 g         1822 t
ORIGIN      
        1 cgctgcgact ccgggttcca gccgtagcgc ggcgagacga cgagcgagtt cgcgaggtcg
       61 gggtactgcc cgaagccgcg cctcgccccg ctcaggatcg tgttgtgcgc gatcgtcacg
      121 ttcgcgggga gcgcgtccgg accgagccgg ttcccgagca cgatcccgtt cggcggcacg
      181 gggaacccgg ggagggccgg gtcggtcgcg cggaagaggt tgtcgcgcac gacgagcccg
      241 tcgatcggcc cggagaggta gagctggccc gcgccgggcg gctcgacgag cccgaagacg
      301 ttccgctcga cgaggacgtt cgtcccggcc tggacctgca cgaggtcgtt gtggttgcag
      361 gcggccggat ccgttccaca gggcccgacg agcgcccgga ggaagcggtt cccgcgcagc
      421 acgaggccgg ttccggcgcg accgcgcacg aagtcgcacc cgaagcagtc ctcgaaccgc
      481 gatccctcga cgaggacgtc cgagctccgg ccgaggagga ggcacacggc gccgtcgccg
      541 cagcgggaga accggctcgc cgtgatcgtc accccgttgc tcgcgtcgag gacgacccgc
      601 gcgcggcgca ccgtcgtacc gcgcacgcgg atcccgtcga gccggacgcc ccggctccgg
      661 cgcacctcga ggctcgccgg gcgagcgtcg gggaggagga cgacgttctc gagccgcacg
      721 ctccgggtct cgcggaggag gatgcggcgc acggagacgc cccgctgcgc gcggacgacg
      781 agcggccgtg ccgagcgcgg gccgacgcgg agcatccggt agcgcccgcc ccgcaggacg
      841 atcgtcccgc cgggccgctt cagccggttc acggcctgct ggaactgccg ctcggtcttc
      901 acgaccaccg gcttcggccg cgccgtcgcc tccgcgggtg cggggagcgc caggcctccc
      961 gcgaggcccg cgagcacgat cgccagcttc gaccccaggt gaatgcgcgc cgcgatcccg
     1021 gcaatgaacc atcctccgcg acgcgattca agacccggcg aaatgctgga agagacgggc
     1081 cggacgcctg cggggtagtg tcccgcccgt gaacaggcag atcgccatcc tcggcgcggg
     1141 gaagatcggc gagtcgctca tctcgggcct cctctcgtcc gagtggcgcg accccggcga
     1201 gatcgtggcc acgtcgcgcc gggacgaccg gctcgccgag ctcgcggagc gctacgggat
     1261 ccggacgacg cgctcgaacg cggaggcggt ggaggaggcg ggcatcgtcg tcatcgcggt
     1321 caagccgcag gacatcgagg gcctcctcga cgagctgcgc ggccggctcc ggccggggca
     1381 gacgatcatc tcgatcgcgg ccgccatccc cacgagcctc atcgaggggt acctcgggga
     1441 cagcgtcgcc gtcgtgcgcg cgatgccgaa cacgcctgcg gcggtgcacg aggggatcgc
     1501 ggggatcgcg gcgggggcga acgcggggga ggatcacctc gccgccgccg aggaggtcct
     1561 ctcgcacctc ggccgcagcg tccgcgtgga cgagagctac atggacgcgg tcacggccgt
     1621 gtccggctcg ggcccggcgt acttcgcgct cctcgcggag tcgatgatcg aggcggggat
     1681 cctgctcggc ctctcgcgcg agatctccac cgatctcgtc gtgcagacga tgctcgggac
     1741 cgcgaagctc ctgcgcgacg aggggatgca cccagtcgag ctccgcgaga tggtcacgtc
     1801 gcccggaggg accacgatcg cggcgatccg ggagctcgag caggcggggg tccgcgccgc
     1861 gttcctgaac gcgatccagg cggcgatgcg ccgctcgcag gagctcgcgg ccggctaggc
     1921 acgccggacg ggcgcccgtt atcctcgccc gcatgacgga agcgacgcgc ttcctgctcg
     1981 gcgagagcga cattcccgcc cgctggtaca acgtccaggc cgacctgccg gagccgctcc
     2041 cgcccgtcat ccaccccggc acggggggtc cgatcggccc ggacgacctc gccccgctct
     2101 tcccgatgga gctgatcggc caggaggtct cccaggagcg ctggatcgac gtccccgagg
     2161 aggtgcggga gatctaccgc ctctggcgcc cgacgccgta cgtccgcgcg cgccggctcg
     2221 aggcggcgct cgagacgccc gcgcgcatct actacaagta cgagggcgtc tcgcccgtcg
     2281 gctcgcacaa ggcgaacacg gccgtcccgc aggccttcta caacaagcgc gagggcgtcg
     2341 gcggcctcac gaccgagacc ggcgccgggc agtgggggag cgcgctcgcg atggcatgtc
     2401 gcttcttcga ccttccgtgc gaggtgttca tggtgcgggt ctcgtacgag cagaagccgt
     2461 accggcggag cctcatgcag acgttcggcg cgacggtcac cccgagcccg tccgacgaga
     2521 cgagcgccgg ccgtgccgtg ctcgcggacg acccgggctc gcccgggtcg ctcggaatcg
     2581 ccatctcgga ggcggtcgag gcggcggcga cgagcggcgg ggcgaagaag tacgcgctcg
     2641 gctccgtcct gaaccacgtg ctcctgcacc agacggtgat cggcgaggag acgctccttc
     2701 agctcgagct cgccggcgac tacccggatc tcgtggtcgg ctgcgtcggc ggcggctcga
     2761 acttcggcgg gttcgcgctc ccgttcgtgc gcgagaacct cgcgggcagg cgctcgacgc
     2821 gcatcgtcgc ggtcgagccg gcggccgcgc cgagcctcac gaaggggcgc tacgtctacg
     2881 acttcgggga cacggcccgg acgacgccgc tcatcaagat gtacacgctc ggacactcgt
     2941 tcgtgccggc gcccgtccac gccggcggcc tccgctacca cgggatggcg ccgatcgtct
     3001 gccacctcta cgacctgggc ctgatcgagg cgcgcgcggt ctcccagaac cccgtcttcg
     3061 acgcggcggt gacgttcgcg cgcgcggagg ggatcgtgcc ggctccggag ccggcgcacg
     3121 cgatccgcgt cgtgatcgac gaggcgctcg cgtgcaagga gtccggcgag gagcggacga
     3181 tcgccttcaa cctctgcggg cacgggcact tcgacatggg cgcgtacgac gagtacctcg
     3241 cggagcggct cccggacttc gagtacgccg aggaggacgt ggagcgcgcc ctcgccgagc
     3301 tgccggccgt cggctagcga agcgcgggcg cgggatcgag cgcggcgccg cggcggcgcg
     3361 cctcgaagtg caggtgcggc ccggtcgccc tgccggtcgc gccgacgcgg ccgagccgct
     3421 cgccgctcgc gacggcctgc ccgggccgca cgcggaaccc gcgcaggtgg gcgtaccagg
     3481 tgtggacgcg gtgcgcgtgg cggacgacga cgagccttcc gtagccgccc gtgtcccagc
     3541 cggcgctgac gacgacgccc ggcgccgcgg cgcgaacggc ccgcccggcg ggcgccgcga
     3601 agtcgagccc ggtgtggaac cggtttccac gcggcccgaa ccggtccgtg agcagccccc
     3661 tgacgggccg gccgagggcg atcgggacgc gcttcagcgg ccgccggagg gcccggtacg
     3721 tcgcggggcc ggggaggccg tccgccagga gccgtgcgta gcgctggaag cggatcacgg
     3781 cagaggtcgt ccgcggcccg aacaccccgt cgagcgctcc tgacgggaac ccgcgccagg
     3841 ccatcctgaa ctggaggacg gagacgtccc agccgacgcg cccgttgcgg aggacgcggc
     3901 tcccgaagtc gtgccgaccg tatcgcccga gcgcccggcg ggtctgcggg cccgcgaccc
     3961 cgtccaccga gagccccgcc cgccgctgca gccggcggac ggcctgctcg gtggccgggc
     4021 cgtagtggcc gtcgatcgtg ccgccgtaga ggcccttcgc ctggagcccg atctggagcg
     4081 cggcaacgcg cggcttgccc atcccttgcg ccggagcggc ggcgagggcg gcgacggcga
     4141 cgacaaggcc gcagaagctg atcgtccgtg ccagaggtga atccccccgc ggacgttgta
     4201 gcagagcgca ctaccgtttt ctagaccggt gtgagaacct ccgcgccgca cccgaagacg
     4261 agcgcagcgc gtgcctcgtc cggcccgacg ccggcgagcg atgggtgtcc gaggtccgcg
     4321 gtgacgtcgc cccaggcgtc gagcccgagc gcggcggcgc cttccacgga gccgatccgg
     4381 aggagctcgt ccacgtcgag cacgccgcgc cgcagctcga ggcggcgcgc gattccctcg
     4441 agctcgcgga gctcctcgag cgggtcgatg cggacgttcg agtcggagcc gacgcagaga
     4501 gggatcccgc gcgcgcggat ccggccgacc gggacgaagc cgtcgccgag gctcgcctcg
     4561 gtcgtcgggc agacgcagat tcgcgcgccg tgctcggcga ggaggtcgag ctcggcgtcg
     4621 gaggcgtgtg tcgcgtggac gaccgacgtg cgcgggccga gacagccggt gcgggcgagg
     4681 agctcgatcg gccgcaggcc gtgctcggcg aggcactcct cgatctcgcg cggctgctcg
     4741 cacgcgtgca cgtggagcgg aagctcgtgc tcggcggcgt acgcgcccac ggcctcgagc
     4801 cagtccggcg ggcacgcgcg cacggagtgg ggggcgaccc cgaccgcgac cccgtcgcgt
     4861 cgcagcgcgg cgacctcctg caggtaggcg tcgaccgtcg gctgccggag ctcgtcgatc
     4921 ccgccgcggg cgtacgcgac gtggagcagg acgaaccgga tcccggcctc cgccgcggcg
     4981 tcggacgccg catacgcctg gtcgaggccc aggtagtgaa actcgccgac cgccgtgaag
     5041 ccggccgcga gcatctcgcg gtagaccgcg acgtagtcct gccggacgcg ttccggggac
     5101 tgtccccggg ccacgtccag catggcgtcc cgccaggccc agaagtcgcc gccgtccacg
     5161 cggccgcgga gcgcccgctg gaacgcgtgc gagtgggcgt tcacgaagcc cggaagcgtc
     5221 agccggccgg ccaccgcgcg aggctaccac cggccgcacc gtccgcttcc ggggccggcg
     5281 gcgggtgggg gatacgatgg ccgcatggca acccgggccg cctcggcgcc ctatgcgcac
     5341 atcccgaacg cgctcacgct cgcgcgcttc gcgctcatcc cggtcttcgt cgcgatggtg
     5401 ctcgccgccg acggggcggg gagctacgcg gccgccgccg tcttcgcgct cgcgggcgcg
     5461 accgaccagg tcgacgggtg gctcgcgcgc cgctggcacg tggagtcccg cttcgggaag
     5521 ctcgccgacc cgctcgccga ccggctgatg atcgacgcgg cggtcgtcct catgtggctc
     5581 gacggccgcc tgccgctcgc ggcgctcgcc ctcatcctcg cgcgcgacgg cgcgctcctc
     5641 ctcggctatc cgctcgtgcg aaaccgcggg tacgacttcg aggtgaactt cctcggcaag
     5701 ctcgcgacct gggtgctcta cgcggcgctc gtcggcgtcg tcgcctcgga cgaggggacg
     5761 acctggccgc tcgtcctctt ctggacgggc gtcgccctcg cggtcgccgc cgcggcccag
     5821 tatctcgcca aggcgctgcg ggaggtgcgc aggtgaaggc cgtcgtcatg gccggcggcg
     5881 agggcacgcg cctgcgcccg ctgacctcga accagccgaa gccgatggtc ccgatcgtcg
     5941 gcaagccgtg catggagcac atcgtcgagc tgctccgccg gcacggcttc gacgacatcg
     6001 tcgtcacgct cgcgttcatg ccgcagatga tccgcggcta cttcggcaac ggcgagcggc
     6061 acggggtcga catccgctac tcggtcgagg agacgcccgc cggcaccgcg ggctcggtga
     6121 agctcgccga ggaggcgctc gacgagccgt tcctcgtcat ctcgggcgac gccctctgcg
     6181 acatcgacct ctccgcgctc gtccgcttcc acgaggagcg ggaggcgctc gtcacgatcg
     6241 cgctcaagag cgtcgacaac ccgctcgagt tcggcatcgt cgtgaccgac gaggacgggc
     6301 gcatcgagcg cttcctcgag aagccgtcgt gggcccaggt cttcacggac acgatcaaca
     6361 ccgggatcta cgtcgtccag ccggaggcgc tccgggacgt cccgaccgac cggccgtacg
     6421 acttcgcgaa ggagctcttc ccgcgcctgc tcgagaaggg ccggccgctc tacggcttcg
     6481 tcgtggacgg ctactggcag gacatcggaa acctcgacca gtaccgcaag gccaacttcg
     6541 acgcgctcga cgaggccgtc gacctcgaga tccccgggat cgtcctgcgc ggcaacgtcc
     6601 acgtggggga gggggtcgag ctcgacgacg tcgagtcggt cgaggggccc gcgtacatcg
     6661 gcaactactg ccggatccac ccggacgcga cgatcggccc gtacacggtg ctctcgtcga
     6721 gcgtcgcgat ccgcgagcag gcgcggatcg agcgctcgat cgtggacagc tcgacgtaca
     6781 tcgggcgaag cgccgtcgtc gagggcgcga tcgtcgggcg gatgtgcgac atccgcgagc
     6841 acgcccggct ccacgagggc tccgcgatcg gcgaccagac gacgatcggc gacgaggccg
     6901 tcgtcttccc cgagatccgc gtctacccgt tcaaggagat cgagtcgggc gcgcaggtcg
     6961 accggaacgt catctgggag gcgcgtgcga ccgcgtcgat cttcgaccgc gagcacatcc
     7021 gcggcctccc caacgtcgat ctcacgccgg agatcgcgct ccggctcggc atggcgctcg
     7081 gcacggcgct caagcgcgac gcgcgcgtcg tcacgagccg ggaggcggcc cccgcgtgcc
     7141 ggctcctccg ccgcgccgtc ctcgcgggga tcaacgcgac cggcgtccac gtcgccgacc
     7201 tgcgcgtcct gccggcggcg gtgaaccgcc acctcgtgaa gagcgagggc tacgaggcgg
     7261 gcgtccacgt cggcacgagc gcgagcgacc ccgaggcggt cgagatccag gtctacgagc
     7321 cgcccggcgt ccaggcgtcg cagtcgttca tgaaggagat cgagaagcac ttctcccgcc
     7381 aggagttccg gcgcgcggcc gcggacgagg tcgggcagct cgtgtttccg gcccgcgcgg
     7441 cggagagcta cgtcgaggat ctcctcgcga cgctcgacgc ggacgcgatc cgcgcgcgcg
     7501 gattccggat cgtcgtcgac tacgggtact cctccgcctc gctcgtcctg ccgctcgtgg
     7561 tcggctcgct cggggtcgag atcgtcgcct cgagcccgta cgtgcgcgac caggtcgcga
     7621 tcccgcaggc cgcgacgatc cgcgagtcgc tgaaccgcgc gtcgcgcctc gtcggcgccg
     7681 tcgccgccga cctcggcgtc gtcctcgacc ccggcgcgga gcggatctac ctcatcgacg
     7741 agggcgggcg cgccgtcccg cccgaccagg agcttctcct gttcgagcgc ctgatcggct
     7801 cgaacggcac gacgggacgc gtcgcgttcc ccgtcacggt cacgtcgctc gtcgaccgga
     7861 tcgccgaggg gacggggctc gaggtcgtcc gcacgccggc ctcgctcgcc gcgctcacgc
     7921 gggcggccgc gcacgacggc gtcgtgttcg ccggctcggc ctcgggcggc ttcgtcttcc
     7981 ccgacttcgt gccgggctac gacgcgatca cgagcctctg caagctcctc gagctgctcg
     8041 ccccgctcgg ccggccgctc tccgaggtcg tggacgaggt cccgcgcccg ctcgtcgcgc
     8101 atcgtgcgat ccggtgcccg tggtcgcgca agggcgccgt catgcgcgtc ctcaccgagc
     8161 aaacgaagga ccgggagacg gacacgctcg acgggctcaa ggtcttcatc gacggcggct
     8221 gggcacaggt gctccccgat cctgccgagc cggtcgtgca cgtgtacgcc gagggcacga
     8281 gcgaggacga gacccaccgg ctcgaggagg agttcacgac gctcgtcgag tcgatcatcc
     8341 aggacgacga cgcctgacgg gcaaaccctc aagccgaggt tgacggttcg gcaccctcct
     8401 gctttacttg agagtccact cttccggagg acgccgtgga accgctcccc gatctgaaca
     8461 gcctctccga ctcggagctc cgcgacctga tcgcgaagct cgtggaggag gagaacgagg
     8521 tcagctaccg ccgtcgcatc ctgcacggca agatcgacat cctgaaggcc gagctccagg
     8581 cgcgcctcca gaggcaggtg ggggaggagg ggcagagccc gctctacgag atcgacgtgg
     8641 acaagctcgc cgacatcctc tcggcccgcg ccgcaccgcc gaaccccgcc gactaggagg
     8701 ggcccgtgac gcacatccac tgtcccgagt gcgggttcca gaacccggag agcgcgaact
     8761 actgctcgaa gtgcggcgcg ctcctcatcc gcgaggagcc gggcgggcac acgacgatgt
     8821 cgttcgcgcc ggaggagctc gccgacgacg agagcggcat ggcgctcgag gacctgcccg
     8881 tcgagggcac ggccctggtc gtccgcgccg gcggcgggcg gcagggcgag acgttcccgc
     8941 tcgatcaggg gcgccacgag atcggccgct cgccggacgc ggagatcttc ctcgacgacg
     9001 tgaccgtctc gcgctcgcac gcgatcgtga cgcgcgacga gtccggcgtc gtgatcgagg
     9061 acctcggcag cctgaacggg acgtacgtca accgccggcg cgtcgacaag gcgaccctcg
     9121 aggacgggga cgaggtgcag atcggcaagt accggctgac cctcctctcg cgctcgtgat
     9181 ggcgacggcg acgcgcgaga agagcggcag gcggctcacg atcggcgcag tgcgcgaccg
     9241 gctcgcggac gagttcccgg acatctcgat ctcgaagatc cgctacctgg agggccgcgg
     9301 cctcctggcg ccgcggcgga cgcagagcgg ctaccggctc tactccgagg acgacgtcga
     9361 gcggctgcgg acgatcctgc gcctccagcg cgacgagttc ctgccgctca aggtgatccg
     9421 cgaggagctc gcctcgcccg gcgccaagcg gcgccaccag cgcaagcgcg aggccgacgg
     9481 cgggatgctc gccgcggccg agccggagat cgacctgcgg acgctctgcg agcgcgccgc
     9541 ggtcgagccg tcgttcgtgc gggagctcga ggagtacggg ctcctcgagc cgcgccgcga
     9601 ggacggagag gcgctctacc cggagcgcga cgccgaggtc gtggccgcct gcgcccgcct
     9661 cgcacgcttc gggatcgagc cgcgcaacct ccgctcgatc aggaacggcg cgtcgtcggc
     9721 ggccgggctc ctcgagcaga tcgtcgcgcc ggccctgcgc tcccagaacc ccgagcggcg
     9781 cctcgccggc ctcgaggacc tcgagacgct cgcggcggcc tcgcaggagc tctcgcagct
     9841 cctcttctgg cgcgccctgc gctcgctcgt ctagggaagg ctctaagctc tgggcgtgac
     9901 cgtcgatctc gaggcgaaga tccgcgacat ccccgacttc ccgaagcagg ggatcgtctt
     9961 caaggacatc atgccgctcc tcgccgacgc ggagacgttc cacgcgacga tcgacctgct
    10021 ggccgagttc ggggagcctc gcaagcccga cctcgtgctc ggcgccgagg cgcgcggctt
    10081 catgctcggc ggggcgctcg cgtacaagct cggctgcggc ttcgtcgccg cgcgcaagcc
    10141 ggggaagctt ccgtgggaga cggtgagcgc cgagtacgcg ctcgagtacg gggtcgactc
    10201 gctcgagctg cacgcggacg cgatccgggc gggcgcgcgc gtcctcgtcc atgacgacct
    10261 gctcgccacg ggcgggacgg cgcgggcgaa ggtggagctc gtcgagcagc tcggcgggga
    10321 ggtcgtcggg ctggcgttcg tgatcgagct cgccttcctg aacggccgcg agaagctcga
    10381 gggctacgac gtccactccc tcatccagta ttgaccgctc gcgtggaggc gccggacggc
    10441 cgataaggga agggacgtgt acgacggcct gacggggcgc tacaccttcc ggtgcccggc
    10501 caccggggag gcccgcgtgc ggctgtcgcg cttccgctcc gtcgagcgcc tcgccggccc
    10561 cgcgcgcccc tccgtcttcc gcgtccgctt cgcctgcccc tgcggggacg accacgaggg
    10621 gctcgtcacg cacgacgagc tcgactgggc gccgctcggg agcgacgcga gcgcgttctt
    10681 caacgtcatg acgcggcgcc tcgagccggc gcgcgacgag ctcctcgagc tcgcggcgcg
    10741 gcggatcggc gcgggggagt ggccgtggac gttcttctgc tggcccgagg ggcgcccgcg
    10801 accggtgttc ccgtccgcgt tccggctgct cgcggaggcg gaggagcgcc tcggcgtcgc
    10861 cgtccgctgc cctgactgcg gcgggacgtc cgtcaacgtg gtcagccgcc gccacgtgga
    10921 cgagccgttc ttcaacgacg acgcggtcgc cgtcgtcgag cacgtcttcg cggccgacgc
    10981 ggaggagctc gtggcgggat ttcgcgccga gctcgactcc gccgcgttcg acgcgcgccg
    11041 cctcgccctt tgagccgagc gttacgcgcc cgccgaatcg gggcaccacg tgccgggggg
    11101 atggtctccc cacgttccga cgacagggag tgagacatgg cattgcttca gcgtaggacc
    11161 gacgagcgcg agaccaccac ggtggacgac gacgtcgcga cggagcgcgc aggggcgagc
    11221 ccggggatcg tccgggctct gttcacgctc gccggggtcg cgggcgccgg cctgctgctg
    11281 tggctcgcgc agctcttcaa cctcgacgag gcgtcgggct tctgggtcgc cgccgggatc
    11341 gtcgccgcag ccggcctggc gctcggcctc tcgcagctct tcggcggctg gacgaagtgg
    11401 ggctggccga cgatgagccc gatgatgttc ctgctcgcgt tcatcccgac gctcgtcgtc
    11461 ggcgggatcc tgctgctcgc gtggcagccc gacggcggcg aagggcagga gttcgcgaac
    11521 gacgtgatct cgaccctcgg cgtgggcggc ctcgtcgatg cgctcgagcc gttcatgtgg
    11581 gtcgtcccgc tcgtgatcgg gctcgtgttc tcgttcacgt tcgacacgac cggcccgcgc
    11641 acccggatcg tcgacaccga cggcacggtg ctccgcgagc gcgtggtcac gcacgaccgc
    11701 gaggccgttc cggacgagga cgtgcacgac taccgcggcg acgacgaccg ccgcgacgac
    11761 gggtcggtcg ccgaggagct ccgcgagcgc gaccgcctgc aagcgacgcg gacgacgacg
    11821 gacggggaca cgcgcgtcga ccgcgaccgc atctagacct ctcgggagat gccggcgggg
    11881 gccctcgagg ttcccgccgg cacgttccct cgcgtcaggg gttggcccgg gcgtggcgcc
    11941 atgccgatag tcagcacatg gacgccgcat ccacgttcgt ccccgagacg gggacgggca
    12001 tgacgaggat cctcggggac tgcgagcagc tcggccgggt gaccggcgat cgaagctcga
    12061 gcgccctccg ccgcctcgag accgtgatcg gcgaggagct cgccggccgc ctcgtcggcg
    12121 cgctcgccgg cggccgccgc tcccgcggct tcccgttcta gggcgcctag cggaactcct
    12181 cgccgccgta gaagagcacg aggaagaaga tcccgagccc ggcgaggagc ccgaggagca
    12241 gcgccgtgcc cacgccgacg ccgacggcca cgaggcccag ccagacgtac gcgccgagcc
    12301 cgaaggccca gaggaacgac gtgaagccgt ggctgaagac gggcgggcgg aggtgcatcg
    12361 cggcgatcct actggacggc cgggcgggag cctcgtgcga ggcggcttcg cgccggcccg
    12421 gccgttaaat aggttcaccg agccggtttc gctttcgtcc acttctcgaa ccgaccttcc
    12481 ccttccgggt accgccgctc cgtgaagctt cgacctactc gcgttccgtg ctcggtgcaa
    12541 gcggcaatct agggcacccg tcccggcccg tcaaggccgg aagaccgaaa tgttcgcaaa
    12601 gagcgcgaaa caattaccgt ccacatattc cacagcaatt tcatccacct gtgggaaatc
    12661 ctgtggataa atagcgcggc ccgccgcggc gctcagggag cgggcgcgag gccgcccggc
    12721 gtgtagacgc cggccgcgtt ccagagcagg tagccggcct tcgtcagctt gcgcgcggcg
    12781 gtgacctggg cctggatctg ctcgagcccg tactggcgtc ccatcgtgaa gtcctgcagc
    12841 caggggacga tgagcgtgtc cgcgccgcgc atgacctcct tgaagtcccg gagcgccatc
    12901 gtgacggtcg cgccgggcgt cccgtccggg tcggggaggt cgtactcgcc ggggctgtag
    12961 tgcgacggat agaccatcgg gtagatcacg tcgaggtggg gggcgaggag ccgcgggcgc
    13021 tgcccgatcc cgaggtcgcg cgtcgcggcg aggccgaaga cggcggcgga gatgcgcacg
    13081 ccgagcggct cgagccggtc gcgcgcgtac gcgaggaagc gtgcgatcgt ctcgcccttc
    13141 ttctcgttga cgcggccggg ccagcgcgcc tgctcgatcg gcccgtcgct cgggaagcgg
    13201 acgtagtcga acatgatctc gtcgaagccg gcgcgcgcgg cggcctcggc gatgtcgacg
    13261 ttgtagtccc agacccgctc gtcgtactgg ttcgaccagc cgagcccgcc gtagttcgtc
    13321 cagatcccgc cggcggagct ctgcacggcg agccccggcc gctcgcgcgc gagataggga
    13381 tcctcgaaga cgacgaggcg gccgatgagg tacaggccct tcgcgtgcac ccgctccgcg
    13441 acgcgctcgg gctcgtagta gtcgcgtgcc gcgccgaccc gcgtcgcgag cggcgcgagc
    13501 ggcgacgcga aggcgacctc gccgttctcg tccttcacgt cgatctggag agccgtcatg
    13561 ccctcgtcgg
//
LOCUS       DASTFC010000241         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_813273,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000241 DASTFC010000000
VERSION     DASTFC010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813273"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFO88_07205"
     CDS             <1..598
                     /locus_tag="VFO88_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9545348.1"
                     /translation="ALCSRLVERAESMRLGDGLDDATDVGPVINQAALEKIHSYTGIG
                     RDEGAKLLTGGELATENGLDRGFFYRPTVFADVEPGMRIAQEEIFGPTTALIRAGDVD
                     EAIRIANGVKYGLSSSIFTRDVNKAFKAMRDLKTGITYINAGTTGAEVHLPFGGTKDT
                     GNGHREAGQAALDFYTEWKSIYVDYSGKLQRAQIDNAE"
     gene            595..915
                     /locus_tag="VFO88_07210"
     CDS             595..915
                     /locus_tag="VFO88_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545349.1"
                     /translation="MKRKLVVFVPREALDTVREALFAAGAGRIGDYARCSWYTQGTGT
                     FLGGETTDPTVGEPGREERVAELRLETVYPVELEAEVVRALREAHPYEEPAFDLYPLA
                     EPPA"
     gene            912..1316
                     /locus_tag="VFO88_07215"
     CDS             912..1316
                     /locus_tag="VFO88_07215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HET9545350.1"
                     /translation="MRARLSTDGGARGNPGPAAYGYVLEAPDGTVLDARGEAIGTATN
                     NVAEYRALLAGLERAVELGVDELEVVSDSELLVKQMRGEYKVKNETLRGLWEDAGRLA
                     RRLGRVEFTAVRREHNELADALVNEALDRAQP"
     gene            complement(1285..>2295)
                     /locus_tag="VFO88_07220"
     CDS             complement(1285..>2295)
                     /locus_tag="VFO88_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545351.1"
                     /translation="LALLGPRDGHSGIFPLDDAHEQELTLYPLRLYAFPDGLFVVSAE
                     DEALVGLELISVAGRPVDEVLALVEPLVPRDNEQSLAARAPQWLVTAEVLAGLGVVGE
                     AAAPIELVFRRGEDDTLATQVEPVPASEYAAGQPDLFHPMVPQGLPGVPGGPAYLARR
                     GEERWAEWVDDGRAVHAAYNVTLGTTSDFALQVLRLAQRPGVRQVILDLRHNPGGNNT
                     TYARLLQALGTVPGLTVLTSRTTFSAAANLLAELERDAEPLLVGEPTGGSPNLYGDPV
                     DVLLPETGLAAHVAGEYWELAPPDDERLAFEPDVPVELSAGDYFAGRDPVLTAALGRA
                     PR"
BASE COUNT          337 a          798 c          880 g          280 t
ORIGIN      
        1 cgcgctctgc tcgcgcctcg tcgagcgggc ggagagcatg cgcctcggcg acggcctcga
       61 cgacgcgacc gacgtcggcc cggtgatcaa ccaggccgcg ctcgagaaga tccactcgta
      121 caccgggatc ggcagggacg agggcgcgaa gctcctcacc ggcggcgagc tggcgacgga
      181 gaacggcctc gacaggggct tcttctatcg cccgaccgtc ttcgccgacg tcgagccggg
      241 catgcggatc gcccaggagg agatcttcgg cccgacgacc gcgctcatcc gagcgggcga
      301 cgtggacgag gcgatccgga tcgcgaacgg cgtgaagtac gggctctcgt cctcgatctt
      361 cacccgcgac gtgaacaagg cgttcaaggc gatgcgcgac ctgaagacgg ggatcacgta
      421 catcaacgcg ggcacgaccg gggccgaggt gcacctcccg ttcggcggga cgaaggacac
      481 cgggaacggc caccgcgagg ccggccaggc ggcgctcgac ttctacacgg agtggaagtc
      541 gatctacgtc gactactcgg gcaagctcca gcgcgcgcag atcgacaacg ccgagtgaag
      601 cgcaagctcg tcgtcttcgt gccgcgcgag gcgctcgaca ccgtccgcga ggcgctcttc
      661 gccgcggggg cgggtcggat cggcgactac gcgcgctgct cctggtacac gcaggggacg
      721 gggacgttcc tgggcgggga gacgaccgac ccgacggtgg gcgagccggg ccgcgaggag
      781 cgggtcgccg agctccggct cgagaccgtt tacccggtgg agctcgaggc cgaggtcgtc
      841 cgcgccctgc gcgaggcgca cccgtacgag gagccggcgt tcgacctgta cccgctcgcc
      901 gagccgccgg cgtgagggct cggctctcga cggacggcgg cgcgcgcggg aacccggggc
      961 cggccgcgta cggctacgtc ctcgaggcgc cggacgggac ggtgctcgac gcgcgcggcg
     1021 aggcgatcgg cacggcgacg aacaacgtgg cggagtaccg cgcgctcctc gcgggcctcg
     1081 agcgcgcggt cgagctcggc gtggacgagc tcgaggtcgt ctccgactcc gagctcctgg
     1141 tgaagcagat gcgcggcgag tacaaggtga agaacgagac gctccgcggt ctctgggagg
     1201 atgcgggacg gctggcgcgg cggcttgggc gggtcgagtt caccgccgtc cgccgcgagc
     1261 acaatgagct cgcggacgcg ctcgtcaacg aggcgctcga ccgagcgcag ccgtgagcac
     1321 cgggtcgcgg cccgcgaagt agtccccggc cgagagctcg accgggacgt cgggctcgaa
     1381 cgcgagccgc tcgtcgtcgg gcggcgcgag ctcccagtac tcgcccgcga cgtgcgcggc
     1441 gaggccggtc tcggggagca ggacgtcgac cgggtcgccg tagaggttcg ggctgccgcc
     1501 ggtgggctcg ccgacgagga gcggctcggc gtcgcgctcg agctcggcga ggaggttcgc
     1561 ggcggccgag aacgtcgttc ggctcgtgag cacggtcagc cccggcacgg tcccgagcgc
     1621 ctggagcagc cgggcgtacg tcgtgttgtt cccgccgggg ttgtggcgca ggtcgaggat
     1681 cacctggcgg acgccgggac gctgcgcgag ccggagcacc tggagcgcga agtcgctcgt
     1741 cgtcccgagc gtgacgttgt aggcggcatg caccgcgcgc ccgtcgtcga cccactccgc
     1801 ccaccgctct tcgccgcggc gggcgaggta cgccggcccg cccggaacgc cgggaagccc
     1861 ctgcgggacc atcgggtgga agaggtccgg ctggccggcc gcgtactcgg acgccggcac
     1921 cggctcgacc tgcgttgcaa gcgtgtcgtc ttccccacgg cggaagacga gctcgatcgg
     1981 cgccgccgcc tccccgacca cgccgagccc cgcgagcacc tcggcggtca cgagccactg
     2041 gggcgcgcgc gccgcgaggc tctgctcgtt gtcgcggggc acgagcggct cgacgagcgc
     2101 gagcacctcg tcgaccggcc gcccggcgac cgagatgagc tcgagcccga ccagggcctc
     2161 gtcctcggcc gagacgacga agagcccgtc cgggaacgcg tagagccgca gcggatagag
     2221 cgtgagctcc tgctcgtgcg cgtcgtcgag cgggaagatc ccgctgtgcc cgtcgcgcgg
     2281 gccgagcagc gcgag
//
LOCUS       DASTFC010000242        16027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_681358,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000242 DASTFC010000000
VERSION     DASTFC010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 16027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16027
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681358"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1548)
                     /locus_tag="VFO88_07225"
     CDS             complement(40..1548)
                     /locus_tag="VFO88_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET9545352.1"
                     /translation="MSPRAAVLVAAPLASGVLLWLASPSVGLWALGWVALVPAGALAL
                     ARPETRAGRAALPLAFGIALELELVPALPFGLADDQWGEPPVPILVAGSPVLVVALVL
                     VPAVTLLLYALRFPQPLPLGRPRSARVALASVAVAATAWTALDVVRTDLDPGGFWGPL
                     FLSQAGAPTARLAALGGPWLVTFALAASGLALAALLVARPRRVALAPLALAAALAVAP
                     LLVDTEPGGDTVRVAAVQPGYDTAEFDLRVNRYLRSVSRDLERASLDLLGDLAPLTRE
                     AAADGAEIVLWPEATLWVDPHENGSVGAALAGLAEETGALIVAPYFLRSESAGAVVAA
                     LPDGTLAPARRKQRPMWYLGEQAADAPAGPLEAGEPALGPLLGVDTQAPGPALRLTDA
                     GAELLLSSTHDWEALAVRQRALARLFAVALGAPLVRADWRHGSAVVAADGSLAADAGL
                     EKRRTALVGEIRVRDGATPYARIGDAFGWACLAAALALFLAAPFLRRRASAG"
     gene            1564..2283
                     /locus_tag="VFO88_07230"
     CDS             1564..2283
                     /locus_tag="VFO88_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9545353.1"
                     /translation="MDAVVMAAGEGRRLRPLTERWAKPILPIDGRPVLATLLRELAAV
                     GPETVWLVTGHLAEQVERLAGDGSAFGLRVRTVRQPDPAGSADAVRRALEAGATAPLL
                     VVAADTVFAAGDLDRARAAWEASGAPGGLGVRPLPPDELGERTRVRVEDGRLAAFGEE
                     SRDEDGAVVTGAPLWFLAPSLAARVPAVPGPPYELAAAFEAALAAGDAVAALSLGPTR
                     DLTRPADLVARNFPYLSGEGP"
     gene            2280..2639
                     /locus_tag="VFO88_07235"
     CDS             2280..2639
                     /locus_tag="VFO88_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9545354.1"
                     /translation="MSETYDLFQQGRDHLKAGRNAQATVALEKAKRREPEKASIREAL
                     GIAYFRIRRFKEAESEFRAMLDLTPVDHYAHFALGRSLEKQGRAREAARHYRLATSLR
                     PQSRDYQRALEGLEGDD"
     gene            2645..3082
                     /gene="dtd"
                     /locus_tag="VFO88_07240"
     CDS             2645..3082
                     /gene="dtd"
                     /locus_tag="VFO88_07240"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET9545355.1"
                     /translation="MRGVVQRVSSAAVEAGGETVAEIGAGLCVLLGIAEGDAEDDAAR
                     LAGRVARLRIFPNDEGRFDRSVLDTGGAALVVSNFTLLADTAKGNRPSFSAAAAPEHA
                     EPLYDRFCTALREEGVAVETGRFGALMTVRIENDGPVTIVLGA"
     gene            3193..3645
                     /locus_tag="VFO88_07245"
     CDS             3193..3645
                     /locus_tag="VFO88_07245"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545356.1"
                     /translation="MTQVHERERELHREVAARVEGALPAVEVLAVELRGPEQLCVYVD
                     RPEGVDHELCVKVTGMLRDYLERYGLEVSSPGPERPLRTREHFAQATGRRVALRTAHE
                     LAGRKRFRGEVVAAGEETVTLAGASGERFEVPYDEIVRGNLIDEGLTR"
     gene            3642..5084
                     /gene="nusA"
                     /locus_tag="VFO88_07250"
     CDS             3642..5084
                     /gene="nusA"
                     /locus_tag="VFO88_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET9545357.1"
                     /translation="MSREILEAVREIEREKGIEEDTLVHALEDALLAAYKKTPGAARH
                     AQVELDDRGDFRVWAVELPADLEERLLEEARERAITELEEAEAANGERSHTLILDEDL
                     DIDWSQVPDDQVERSDVTPEDFGRIAAQTAKQVIQQRIREAERAMMYDEYVDRVTEVV
                     TGIVQQAGDRNNILVDLGKVEALLPRSEQVDGERYEQGSRIKAVITEVRSGTKGPQVI
                     LSRRDPELIKTLFELEVPEIADGLVEIRGVAREPGYRSKIAVESHAQGVDPVGACVGP
                     RGSRVRMVVSELRGEKIDIIPWNAEPARFIAKALSPARVREVLVDDETRESTVVVPDD
                     QLSLAIGKEGLNARLAARLTGWKVDIESETEFARAEAEAAFGGDAAAGEDFSGRCAAI
                     LSNGKRCPNTAVPGSRYCGIPAHRELAAREEAGEVVENAAPVAADEPAEAEAAEAVAD
                     APVAETEAEAEAIAEAVEAAPEEAEAPAES"
     gene            5339..7333
                     /gene="infB"
                     /locus_tag="VFO88_07255"
     CDS             5339..7333
                     /gene="infB"
                     /locus_tag="VFO88_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET9545358.1"
                     /translation="MADRQRPLRPRGGSPGGRRRRVVIDSQAARPRPDQRQRPGEQQS
                     KPRQERQPAPQPTGPATIASGATVKDVSAALGEPVAKIMKFLMEAGEMVTITQSLSDE
                     AIELIAAELKREVTIKHADDEELEPEEYEDDPADLAPRPPVVTIMGHVDHGKTSLLDA
                     LRESAVAESEAGGITQHIGAYQVDHDGRRITFLDTPGHEAFTALRARGAKVTDIAVLV
                     VAADDGVMPQTVESINHARAAEVPIVVAVNKIDKEGANTQKVRQELVTHELQPEEWGG
                     QTQFADVSAKTHQGLEELLERILLVADAELTLEANPKAEASGPIIESRLDVGRGPVAT
                     MLVQRGSVRVGDAIVAGDAWGKVKALHDYRGERVKEAGPGTPVEILGFDKPPSAGEHA
                     RVVENDRQARHLAQLRGQRVRAEQLARRGKGGVSLEDLFTQIEAGAVQELNIVVKADV
                     QGSIEAVTGELAKIDHPEVGVNVIHTGIGAISESDVILASASNAVVVGFNVRPNAEAR
                     ALAEREGVDIRTYRVIYQLTEDIQQALVGMLSPERVEETVGEAEVRALFRASRVGMIA
                     GCYVTTGVARRNAQVRVVREGTVIYETQVASLKRFKDDVREVQTGFECGILLENFNDL
                     KEGDVLEFYETREIERTDLSAGNGGPPAASADGDDAAADE"
     gene            7349..7651
                     /locus_tag="VFO88_07260"
     CDS             7349..7651
                     /locus_tag="VFO88_07260"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET9545359.1"
                     /translation="MAAGYVGILAVELHFPEAGSLKGKRRFVRSAKAQLVNRFGASVA
                     EVAHHDLWQRAGLTVACVAREAHEAERLLDEAERYLHGQEFELVRAARELVAVEAP"
     gene            7648..8004
                     /gene="rbfA"
                     /locus_tag="VFO88_07265"
     CDS             7648..8004
                     /gene="rbfA"
                     /locus_tag="VFO88_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9545360.1"
                     /translation="MSDRMRRVNEALREVLSEAVGDLKDPRIGFVTVTGVDTSRDLRH
                     AVVYVSVLGSERKRKATLAGLESSRGLLQARVNRELRIKRTPQLAFEYDPTVERGVRL
                     TQLIDDLSPGDRDADD"
     gene            7997..8989
                     /locus_tag="VFO88_07270"
     CDS             7997..8989
                     /locus_tag="VFO88_07270"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET9545361.1"
                     /translation="MTELEQVVAALREHDRFVVTTHENPDGDALGSLLATTLALRAAG
                     MDAEMYLFGDLPLPDEYRFMELADLRRGGAPDLTGRVLVAVDCANESRLGRDSERLLG
                     TAELVVNIDHHHDNTRFGDVNLIVPNASSTGEILRDVYRELGVELTPEIAEALYIAVV
                     TDTGRFQYSNTSPKSLRLAAELVEAGADVHRVFQGIYENVAFAKLKLLARALERAEVL
                     EGGRVIVSHLEREDFERAGAEEPFSEGIIDFLRAVEGAEIAALIRQPPTLNGPGRRVS
                     LRTTDEGVDVSAIARKSGGGGHRQAAGFPSEASVAEITDFIRREFLAQRAATAS"
     gene            9111..9947
                     /gene="truB"
                     /locus_tag="VFO88_07275"
     CDS             9111..9947
                     /gene="truB"
                     /locus_tag="VFO88_07275"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9545362.1"
                     /translation="MPHTGADDPGAATGGARDPRGGLVLVDKERGPSSFAVVRRVRNR
                     FGLKAGHAGTLDPLATGLLLCLVGRSTRLARYLVGLDKRYETTIRLGARTTTGDAEGD
                     ELEQTEPPDRAAIEALAGEVELPIPAASAVKIDGERAYRLHRRGVAVEMPTRRSTIHA
                     LEILRLEPPELELALHVSSGTYVRSVADALGGHCLTLRRTAVGPFRVEDADEERLRPP
                     LAAVAHLPLRELGPDEARAAATGAAVPYEGEGAVALVAAGGLVAVARGDGRQARPETV
                     LA"
     gene            9944..10831
                     /gene="ribF"
                     /locus_tag="VFO88_07280"
     CDS             9944..10831
                     /gene="ribF"
                     /locus_tag="VFO88_07280"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HET9545363.1"
                     /translation="MNVLRTPAQADPAERAVAVGTFDGVHLGHRAVLAAAAGTGLRPA
                     VVTFDPHPRVVLGYEVQLLTTLERRLELLAESGPEDILLVEFTPELSRLTPEEFAEAV
                     LRPFGARVVVAGEGFRFGAGRRGDLDLLRGLGFDARAVPLVEEISSSRIREALRDGDI
                     PLAARLLGRPPELEGPVVAGDQRGGTLGFPTANLAVPRDLLVPGYGIYAGAARLADAP
                     ADAHRTALSIGVNPHYGGVERRIEAFLLDFEGDLYGRRLRIELWQRLREERAFASEAE
                     LVAQIAADVEAARAARPPG"
     gene            complement(11018..12469)
                     /locus_tag="VFO88_07285"
     CDS             complement(11018..12469)
                     /locus_tag="VFO88_07285"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9545364.1"
                     /translation="MTPTTSLPPGIAAGHPATAAVGLEALADGGTAADAAVAASLASC
                     AVEVVMTGLAGGGHAIWWDAGRGRAEHLDFFVAMPGLGGARPDAEVVELAVPFGSEIV
                     HYAIGIASCGVPGVVAGLDELWRRHGRLSWERLCAPAIAFARDGVALPPAHAACLAML
                     APVMTMREGAEIFLREDGVLKDAGDELRQPGLARALEAVAGEGPGAFRPDGTLGRALL
                     ALCDERNGLVTADDLAAYRPVWREPAELAYAGARIVTRDALTPLAPVLSSLPRLRDAP
                     PARRVLALARALSGPDVVGSGHTTNLVTADAAGNVCVLTTSLGLGSGDFLPEFQVHLN
                     SMLGESDLLGARLEPGERMASMMSPLVALDAAGVALAAGSAGGTRIRSALAQVTAGVL
                     DEGLPPQDAVDRPRLHRVERVVHVEPGFEPEALEALEGAGLTVTPWPARHHYFGGVSL
                     VSRAGGGADPRRSGAALALEAAAGAGPAGSQPR"
     gene            12532..12801
                     /gene="rpsO"
                     /locus_tag="VFO88_07290"
     CDS             12532..12801
                     /gene="rpsO"
                     /locus_tag="VFO88_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET9545365.1"
                     /translation="MALTKDKKQEVVGTYGRSEADTGSPEVQIALLTRRIEELTEHLR
                     THKKDHHSRRGLLKLVGQRRRLLNYLQKRNLEGYRTLIKELGLRR"
     gene            12909..15383
                     /locus_tag="VFO88_07295"
     CDS             12909..15383
                     /locus_tag="VFO88_07295"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9545366.1"
                     /translation="METTLAPSVADVTVTIGDAEISFETGRLAKQADGAVLVRSGDTM
                     VLATAQGRPEAREGADFFPLTVDVEERMYAAGKIPGGFFKREGRASERATLTARMIDR
                     PIRPLWPKGFRNEVQVICTVLSADLVTPHDILCLNGASAALMLSPLPFAGPVGAVRVG
                     LSEGRLVVNPSLPDLEAESDLDLIVVGTKEGLTMVEAGANRVAEDTLLDALELAHEEI
                     RKLCAVQEELRERAGKAKWLDPATTAEIEEQHAGRIRERIQSAGLREAAAASEEIQAD
                     LAPPITMDSGEEEAIRGLQVKMSLALVLERERLEAVKGPVREQFETELRGLTDAEQDS
                     KQLKSAKRQLLFDRIVETVELPFPAGAPVEGDGPALKDPLTKGFVKKAAEAIYKELVR
                     TKIAVDKRRPDGRGAEEIRSITCDVGVSPRTHGSGLFTRGQTQIMSLLTLGTAKEGQR
                     IDDLSLEEERRFMHHYNFPPYSVGETGFMRGPKRRDIGHGALAQRALEPVIPGPEEFP
                     YTIRIVSETLESNGSSSMGSVCGSTLALMDAGVPIAEPVSGIAMGLVKEGDDYVILTD
                     IQGAEDHLGDMDFKVAGTRDGITALQMDIKITGVTQEIMRSALEQAKRARVAIMDSML
                     EAIPEARTELAAHAPRISTMQIDPDQIGLVIGKGGETIRSLESDYDVQIDIEEDGTLL
                     IYATEGTKADEAIGAIKALTKEPEPGDEYTGKVVKTTDFGAFVELKKGTDGLLHVSNV
                     GPGRVAHIEDVISRGDVLDVKVLEVDKQRGRIGLKLVAKHENGDLVQPETLIERAKDA
                     PPREREERPPRDRERGGRGGRGRDRR"
     gene            15566..>16027
                     /locus_tag="VFO88_07300"
     CDS             15566..>16027
                     /locus_tag="VFO88_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9545367.1"
                     /translation="MRRWDAIVVGAGPAGSSTAFRLAEAGASVLLLDRASFPRDKPCG
                     GGVTPRALEHVPVPIDPVVEHVTTAVELRLGFRRAAVRGRGGPLAYMTQRRRLDALLA
                     ERAAGAGAELRDGVKVTGVEAGGGGVVVTAGGERLHGRTVVGADGVNGVTAR"
BASE COUNT         2271 a         5827 c         6073 g         1856 t
ORIGIN      
        1 ggagcggcct cggcgtcggg gagcggctgc gcggcgcggt cagcctgcgc tcgctcgccg
       61 cctgaggaac ggcgcggcga ggaagagagc gagcgccgcc gcgaggcagg cccagccgaa
      121 cgcgtctccg atccgggcgt acggcgtggc gccgtcccgc actcggatct cgccgacgag
      181 cgccgtgcgc cgcttctcca gcccggcgtc cgcggcgagc gagccgtccg ccgcgacgac
      241 cgcggagccg tgccgccagt ccgcgcgcac gagcggcgcc ccgagcgcta cggcgaagag
      301 ccgcgcgagc gcgcgctggc gcacggcgag cgcctcccag tcgtgggtcg aggagaggag
      361 gagctccgcg cccgcgtccg tcagccgcag cgccgggccg ggcgcctgtg tgtcgacgcc
      421 gaggagcggc ccgagcgccg gctcgccggc ctcgagcggc ccggccggcg cgtccgcggc
      481 ctgctcgccg aggtaccaca tcggccgctg cttccgccgg gccggcgcga gcgtgccgtc
      541 gggcagcgcg gcgacgacgg cgccggcgct ctcgcttcgc aggaagtacg gggcgacgat
      601 cagcgcgccg gtctcctccg cgaggccggc gagcgcagcc cccacgctcc cgttctcgtg
      661 cgggtcgacc cagagcgtcg cctccggcca gaggacgatc tcggccccgt cggcggccgc
      721 ctcgcgcgtg agcggcgcga ggtcgccgag caggtcgagg ctcgcccgct cgaggtcccg
      781 gctcaccgag cgcaggtagc ggttcacgcg caggtcgaac tcggccgtgt cgtagccggg
      841 ctgcacggcg gcgacgcgaa ccgtgtcgcc gcccggctcc gtgtcgacga gcaggggcgc
      901 gacggcgagg gcggcggcga gcgcgagcgg cgcgagcgcg acccgccggg gccgcgcgac
      961 gaggagcgcc gcgagcgcga gccccgaggc ggcgagcgcg aacgtcacga gccaggggcc
     1021 gccgagcgcc gcaagacgcg cggtcggggc tcccgcctgc gagaggaaga gcggccccca
     1081 gaacccgccc gggtcgaggt ccgtccgcac gacgtcgagc gccgtccagg ccgtcgccgc
     1141 cacggcgacg gaggcgagcg cgacccgtgc gctgcgcggc cgtcccagcg ggagcggctg
     1201 cgggaagcgc agcgcgtaga ggaggagcgt caccgccggc acgaggacga gcgcgacgac
     1261 gagcaccggc gagccggcga cgaggatcgg gaccggcggc tcgccccact ggtcgtccgc
     1321 gagcccgaac gggagcgccg gcacgagctc gagctcgagc gcgatcccga acgcgagcgg
     1381 cagcgccgcc cggcccgccc gcgtctcggg ccgcgcgagc gcgagcgcgc ccgccgggac
     1441 gagcgcgacc cagccgagcg cccagagccc gaccgagggc gaggcgagcc acaggaggac
     1501 gccgctcgcg agcggcgcgg caacgaggac ggcggcgcgc ggggacatgc gaccatgatc
     1561 ctcgtggatg ccgtcgtcat ggccgcgggg gaggggcggc ggctccggcc gctcacggag
     1621 cgctgggcaa agccgatcct gccgatcgac gggcgtcccg tgctcgccac gctcctccgc
     1681 gagctcgccg ccgtcgggcc ggagaccgtc tggctcgtga ccggccacct cgccgagcag
     1741 gtcgaacggc tcgcgggcga cggctcggcg ttcggcctgc gcgtgcggac ggtgcgccag
     1801 ccggatccgg ccgggtcggc cgacgcggtg cgccgcgcgc tcgaggcggg cgcgaccgcg
     1861 ccgctcctcg tcgtcgccgc cgacaccgtc ttcgcggccg gcgacctcga ccgggcgcgc
     1921 gcggcgtggg aggcgtcggg cgcgccgggc ggcctcggcg tccgcccgct ccctccggac
     1981 gagctcggcg agcgaacccg cgtccgcgtc gaggacggca ggctcgccgc cttcggcgag
     2041 gagagccgcg acgaggacgg cgccgtcgtg acgggcgcgc cgctctggtt cctcgccccg
     2101 tcgctcgccg cccgggtgcc cgccgtcccc ggcccgccgt acgagctcgc cgccgcgttc
     2161 gaggccgcgc tcgccgccgg cgacgccgtc gccgcccttt cgctcggccc gacgcgcgac
     2221 ctcacgcgcc cggccgacct cgtcgctcgc aactttccct atctttcggg agaggggcca
     2281 tgagcgagac ctacgacctc ttccagcagg gacgcgacca cctcaaggcg gggcggaacg
     2341 cgcaggccac cgtcgcgctc gagaaggcga agcggcgcga gccggagaag gcctcgatcc
     2401 gcgaggcgct cgggatcgcg tacttccgca tccggcgctt caaggaggcg gagagcgagt
     2461 tccgcgccat gctcgacctg acgccggtcg accactacgc ccacttcgcg ctcggccgct
     2521 cgctcgagaa gcagggccgc gcccgggagg ccgcccgcca ctaccggctc gccacctcgc
     2581 tccgccccca gagccgcgac taccagcgcg cgctcgaggg gctcgaaggc gacgactagg
     2641 ccgggtgcgc ggggtcgtcc agcgcgtctc gtccgccgcg gtcgaggccg gcggcgagac
     2701 ggtcgccgag atcggcgccg gcctctgcgt cctgctcggg atcgcggagg gcgacgcgga
     2761 ggacgacgcc gcccgcctcg ccggccgggt cgcgcgcctg cggatcttcc cgaacgacga
     2821 gggccgcttc gaccggagcg tcctcgacac gggcggcgcc gcgctcgtcg tcagcaactt
     2881 cacgctcctc gcggacaccg ccaagggcaa ccgcccgagc ttctccgccg ccgccgcgcc
     2941 cgagcatgcg gaaccgctgt acgaccgctt ctgcaccgcg ctccgcgagg aaggcgtggc
     3001 cgtcgagacc gggcgcttcg gtgcgctaat gaccgtccgg atcgagaacg acgggccggt
     3061 cacgatcgtg ctcggcgcct gagcgccgag agcagcgaaa acgcgcgctc ttgctatcct
     3121 cgaaacgaca tttccccggc tgttggagaa atgggcgcgc ggcgcccatt tttcgtcaga
     3181 ggagcaaaac gcatgacgca ggttcacgag agagagcgcg agctgcaccg cgaggtggcc
     3241 gcccgggtcg agggcgcgct gcccgccgtc gaggtgctcg ccgtcgagct gcgcgggccc
     3301 gagcagctct gcgtctacgt ggaccggccg gagggcgtcg accacgagct gtgcgtcaag
     3361 gtgacgggca tgctccgcga ctacctcgag cgctacgggc tcgaggtgtc gtcgccgggg
     3421 ccggagcggc cgctccgcac gcgcgagcac ttcgcgcagg cgacgggccg cagggtcgcg
     3481 ctccgcacgg cgcacgagct ggccgggcgc aagcgcttcc gcggcgaggt cgtcgcggcg
     3541 ggcgaggaga cggtgacgct ggccggcgcg tccggcgagc ggttcgaggt gccgtacgac
     3601 gagatcgtgc gggggaatct gatcgacgaa gggttgacga gatgagccga gagatcctgg
     3661 aggccgtcag ggagatcgag cgcgagaagg ggatcgagga ggacaccctc gtccacgcgc
     3721 tcgaggacgc cctgctcgcg gcctacaaga agacaccggg cgcggcccgc cacgcgcagg
     3781 tcgagctcga cgaccgcggg gacttccgcg tctgggcggt cgagcttccc gcggacctcg
     3841 aggagcgcct gctcgaggag gcgcgcgagc gcgcgatcac cgagctcgag gaggcggagg
     3901 ccgcgaacgg cgagcgctcg catacgctca tcctcgacga ggacctggac atcgactggt
     3961 cgcaggtgcc ggacgaccag gtcgagcgct ccgacgtgac gcccgaggac ttcggtcgga
     4021 tcgccgcgca gacggcgaag caggtcatcc agcagcggat ccgcgaggcc gagcgcgcca
     4081 tgatgtacga cgagtacgtc gaccgcgtga ccgaggtcgt gaccgggatc gtccagcagg
     4141 ccggcgaccg gaacaacatc ctcgtcgacc tcggcaaggt cgaggcgctc ctgccgcgct
     4201 ccgagcaggt ggacggcgag cgctacgagc agggctcgcg catcaaggcc gtgatcaccg
     4261 aggtgcgctc ggggacgaag gggccgcagg tcatcctctc gcggcgcgac ccggagctga
     4321 tcaagacgct cttcgagctc gaggtgccgg agatcgccga cgggctcgtc gagatccgcg
     4381 gcgtcgcccg cgagccgggc taccgctcga agatcgccgt cgagtcgcac gcccagggcg
     4441 tcgacccggt cggcgcgtgc gtcgggccgc gcggctcgcg cgtccgcatg gtcgtgtccg
     4501 agctccgcgg cgagaagatc gacatcatcc cgtggaacgc ggagccggcg cgcttcatcg
     4561 cgaaggcgct gtcgccggcc cgcgtccgcg aggtgctcgt cgacgacgag acccgcgagt
     4621 cgaccgtcgt cgtccccgac gaccagctct cgctcgcgat cggcaaggag ggcctgaacg
     4681 cccgcctcgc cgcgcggctc acgggctgga aggtcgacat cgagtccgag acggagttcg
     4741 cccgtgccga ggccgaggcg gccttcggcg gcgacgcggc cgccggggag gacttctccg
     4801 gccgctgcgc cgcgatcctc tcgaacggca agcgctgccc gaacaccgcc gtgcccggct
     4861 cccgctactg cgggatcccg gcgcaccggg agctcgcggc gcgcgaggag gcgggcgagg
     4921 tggtcgagaa cgccgctccg gttgcggcgg acgagccggc cgaggcagag gccgccgagg
     4981 ccgtcgccga cgcaccggtt gccgagacgg aggcggaggc cgaggcgatc gccgaggccg
     5041 tcgaggccgc cccggaggag gcggaggccc ctgccgagtc gtgacggagc cggtcaggac
     5101 gtgcgccggg tgcgggcgga agcgtccgca gcgggagctc gtgcgctaca ccgcgccgga
     5161 cgggacgctt gtcgcggaca gtgggcggcg cgaccccggg cggggcgcct acacctgccc
     5221 ggacgaggcg tgcctcgcac gcgcggctgc gcgtggcggg ctcgcccgga cgcttcggcg
     5281 ccgggtccgc gtcccggacg ggttgaaggc acgatcgggg gaggagtagg ctcgaacgat
     5341 ggctgaccgg cagaggccac tgcgtccgag gggcggctcg ccaggcgggc gccgccggcg
     5401 cgtcgtcatc gactcgcagg ccgcgcgtcc gcgccccgac cagcgccagc gccccggcga
     5461 gcagcagtcg aagccgcgcc aggagcgcca gccggcgccg cagccgacgg ggcccgcgac
     5521 catcgcctcg ggcgcgacgg tcaaggacgt ctcggccgcg ctcggcgagc cggtggcgaa
     5581 gatcatgaag ttcctcatgg aggcggggga gatggtcacc atcacccagt cgctctccga
     5641 cgaggcgatc gagctgatcg ccgccgagct caagcgcgag gtgacgatca agcacgcgga
     5701 cgacgaggag ctcgagcccg aggagtacga ggacgacccg gccgacctcg cgccgcgccc
     5761 gccggtcgtg acgatcatgg gccacgtcga ccacgggaag acgtcgctcc tcgacgcgct
     5821 ccgcgagtcc gcggtcgccg agagcgaggc gggcgggatc acgcagcaca tcggcgctta
     5881 ccaggtcgac cacgacggcc gccggatcac gttcctcgac acgccgggcc acgaggcgtt
     5941 caccgccctc cgcgcccgcg gcgcgaaggt gacggacatc gcggtgctcg tcgtggccgc
     6001 ggacgacggc gtcatgccgc agaccgtcga gtcgatcaac cacgcgcgtg cggccgaggt
     6061 gccgatcgtc gtcgccgtga acaagatcga caaggagggc gcgaacacgc agaaggtgcg
     6121 gcaggagctc gtcacccacg agctccagcc ggaggagtgg ggcggccaga cgcagttcgc
     6181 ggacgtctcc gcgaagacgc accagggcct cgaggagctg ctcgagcgga tcctcctcgt
     6241 cgccgacgcc gagctgacgc tcgaggcgaa cccgaaggcc gaggcctccg ggccgatcat
     6301 cgagagccgc ctcgacgtcg ggcgcgggcc cgtcgcgacg atgctcgtcc agcgcggcag
     6361 cgtccgcgtc ggcgacgcga tcgtcgccgg ggacgcctgg gggaaggtga aggcgctcca
     6421 cgactaccgc ggcgagcgcg tgaaggaggc ggggcccggc acgccggtcg agatcctcgg
     6481 cttcgacaag ccgccgtcgg cgggcgagca cgcgcgcgtc gtcgagaacg accgccaggc
     6541 gcggcacctc gcgcagctcc gcggccagcg cgtgcgggcc gagcagctcg cgcgtcgcgg
     6601 caagggcggc gtctcgctcg aggacctctt cacgcagatc gaggccggcg ccgtccagga
     6661 gctgaacatc gtcgtcaagg cggacgtcca ggggtcgatc gaggccgtga ccggcgagct
     6721 cgcgaagatc gaccacccgg aggtcggcgt gaacgtcatc cacacgggga tcggcgcgat
     6781 ctccgagtcg gacgtcatcc tcgcgtcggc gtcgaacgcg gtcgtcgtcg ggttcaacgt
     6841 gcggccgaac gccgaggcgc gcgcgctcgc cgagcgagag ggcgtcgaca tccgcaccta
     6901 ccgcgtcatc taccagctca ccgaggacat ccagcaggcg ctcgtcggga tgctctcgcc
     6961 ggagcgggtc gaggagacgg tcggcgaggc cgaggtgcgc gccctcttcc gcgcgtcgcg
     7021 cgtcgggatg atcgccgggt gctacgtcac gaccggcgtc gcgcgccgga acgcccaggt
     7081 gcgcgtcgtc cgcgagggca ccgtcatcta cgagacgcag gtcgcgagcc tgaagcgctt
     7141 caaggacgac gtccgcgagg tgcagaccgg cttcgagtgc gggatcctgc tcgagaactt
     7201 caacgacctg aaggaaggcg acgtgctcga gttctacgag acgcgcgaga tcgagcgcac
     7261 cgacctctcg gccggaaacg gcgggccgcc cgcggcgagc gccgacggcg acgacgccgc
     7321 ggccgacgag tagccgcggg agacgcccgt ggcggccggc tacgtcggca tcctcgcggt
     7381 cgagctccac ttccccgagg cgggctcgct caaggggaag cggcggttcg tgcgctccgc
     7441 gaaggcccag ctcgtgaacc gcttcggcgc gtccgtcgca gaggtcgcgc accacgacct
     7501 ctggcagcgc gccgggctca cggtcgcgtg cgtcgcgcgc gaggcgcacg aggcggagcg
     7561 gctcctcgac gaggccgagc gctacctcca cgggcaggag ttcgagctcg tgcgggccgc
     7621 gcgcgagctc gtcgcggtgg aggcgccgtg agcgaccgca tgcggcgggt gaacgaggcg
     7681 cttcgcgagg tgctctcgga ggcggtcggc gacctcaagg atccgcggat cgggttcgtg
     7741 accgtcaccg gcgtggacac gagccgggac ctccgacacg cggtcgtgta cgtgagcgtc
     7801 ctcggctcgg agcgcaagcg gaaggcgacg ctcgccgggc tcgagtcctc gcgcgggctc
     7861 ctccaggcgc gggtgaacag agagctgagg atcaagcgga ccccgcagct tgcgttcgag
     7921 tacgatccga cggtcgagcg cggggtcagg ctcacgcagc tgatcgacga cttatcgccc
     7981 ggtgaccgag acgcagatga ctgagctcga acaggtcgtc gccgcgctcc gcgagcacga
     8041 ccgcttcgtc gtgaccacgc acgagaaccc cgacggggac gcgctcggct cgctcctcgc
     8101 gacgaccctc gcgctgcgcg cggccggcat ggacgcggag atgtacctct tcggcgacct
     8161 cccgctcccg gacgagtacc ggttcatgga gctcgccgac cttcgccgcg ggggggcgcc
     8221 ggacctgacc ggtcgcgtgc tcgtcgccgt cgactgcgcg aacgagagcc ggctcggcag
     8281 ggactccgag aggcttctcg ggacggcgga gctcgtcgtg aacatcgacc accaccacga
     8341 caacacgcgc ttcggcgacg tgaacctcat cgtgccgaac gcctcgtcga ccggcgagat
     8401 cctgcgggac gtgtaccgcg agctcggcgt cgagctcacc ccggagatcg cggaggcgct
     8461 ctacatcgcc gtcgtgaccg acacggggcg gttccagtac tcgaacacga gcccgaagtc
     8521 gctccgcctc gccgccgagc tcgtcgaggc gggcgccgac gtgcaccggg tcttccaggg
     8581 gatctacgag aacgtcgcgt tcgcgaagct gaagctcctc gcgcgggcgc tcgagcgggc
     8641 cgaggtgctc gagggcgggc gcgtgatcgt ctcgcacctc gagcgcgagg acttcgagcg
     8701 ggcgggggcg gaggagccgt tctccgaggg gatcatcgac ttcctgcgcg ccgtcgaggg
     8761 cgccgagatc gcggcgctca tccgccagcc gccgacgctg aacgggccgg ggcgccgcgt
     8821 cagcctgcgg acgaccgacg agggcgtcga cgtctcggcg atcgcgcgca agagcggcgg
     8881 cggcggccac cggcaggcgg ccggcttccc gtccgaggcg agcgtcgcgg agatcacgga
     8941 cttcatccgg cgcgagttcc tcgcgcagcg cgccgcgacc gcgtcctgat ccccggtgga
     9001 cgcgcgcccg ggtaacggga ggcgccccca cccctcgaga ttcatcgtat cggggaaaat
     9061 cgcgcggatg tgtgacggac tgcgccgaaa tgcgcacgaa accgtgccgg atgccgcaca
     9121 cgggtgctga cgacccgggc gccgcgacgg gcggcgcgcg cgatccgcgg ggcggtctcg
     9181 tgctcgtgga caaggagcgg gggccctcgt ccttcgcggt cgtgcgccgc gtgcggaacc
     9241 gcttcggcct gaaggcgggc catgcgggca cgctcgaccc gctcgcgacg gggcttctcc
     9301 tctgcctcgt cggacgctcg acgcggctcg cgcgctacct cgtcggcctc gacaagcgct
     9361 acgagacgac gatccggcta ggcgcccgca cgacgaccgg cgacgcggag ggcgacgagc
     9421 tcgagcagac ggagccgccg gaccgtgccg cgatcgaggc gctcgccggc gaggtcgagc
     9481 tgccgatccc ggccgcgtcg gcggtcaaga tcgacggcga gcgcgcgtac cggctccacc
     9541 ggcgcggcgt cgcggtcgag atgccgacgc gccgctcgac gatccacgcg ctcgagatcc
     9601 tccgcctcga gccgcccgag ctggagctcg cgctgcacgt ctcgagcggc acgtacgtcc
     9661 gctcggtcgc cgacgccctc ggcggccact gcctcacgct ccgccgcacg gccgtcggcc
     9721 cgttccgggt cgaggacgcg gacgaggagc gcctgcggcc gccgctcgcg gccgtcgcgc
     9781 acctgccgct ccgcgagctg ggcccggacg aggcgcgcgc cgccgcgacc ggggccgcgg
     9841 tcccgtacga gggcgagggc gccgtggcgc tcgtggccgc gggcgggctc gtcgcggtcg
     9901 cgcgcgggga cggccgccaa gcgcgcccgg agacggtgct cgcgtgaacg tcctccgcac
     9961 acccgcgcag gcggaccccg ccgagcgcgc ggtcgcggtc ggcacgttcg acggcgtcca
    10021 tctcggccat cgcgccgtcc tcgccgcggc cgccgggacc ggcctgcgtc ccgccgtcgt
    10081 cacgttcgac ccgcaccccc gcgtggtgct cggctacgag gtccagctcc tgacgacgct
    10141 cgagcgccgg ctcgagctcc tcgccgagtc gggccccgag gacatcctgc tcgtcgagtt
    10201 cacgcccgag ctctcccgcc tcacgccgga ggagttcgcc gaggccgtcc tgcggccgtt
    10261 cggcgcccgg gtggtcgtcg ccggcgaggg cttccgtttc ggcgccggac gcaggggcga
    10321 cctcgacctc ctgcgcgggc tcgggttcga cgcgcgcgcc gtcccgctcg tcgaggagat
    10381 ctcgtcgagc cggatccggg aggcgctccg cgacggcgac atccccctgg cggcgcggct
    10441 gctcgggcga ccgccggagc tcgagggccc ggtcgtcgcg ggcgaccagc gcggcggcac
    10501 gctcggcttc ccgacggcca acctcgcggt tccgcgcgac cttctcgtgc ccggctacgg
    10561 gatctacgcc ggcgccgcgc gtcttgccga tgcgcctgcg gacgcgcacc gcacggccct
    10621 ttcgatcggc gtgaacccgc actacggcgg cgtcgagcgg cgcatcgagg ccttcctgct
    10681 cgacttcgag ggcgacctgt acggacggcg gctccggatc gagctctggc agcgcctccg
    10741 cgaggagcgc gccttcgcga gcgaggccga gctcgtggcg cagatcgcgg ccgacgtcga
    10801 ggccgcccgg gccgcccggc cgccgggctg acggacggtc gacagttgta aagactttgc
    10861 cggggcgtgc tggcgtcttc cggcgggtgt acgacaatcg gtgcgtggag ccgcgcaccc
    10921 tcgtgaggac ggtcaactgc ctctcctgcg gggagacgta cggcaagccg gagggacgcg
    10981 ggacggtgca gacgaacccc ggctgccccg agtgcggcta cctcggctgg gagccggccg
    11041 gcccggcgcc cgccgccgcc tcgagcgcga gcgccgcgcc gctcctgcgc ggatcggcgc
    11101 cgcctccggc cctgctcacg aggctcacgc cgccgaagta gtggtgccgc gccggccacg
    11161 gcgtgaccgt gagcccggcg ccctcgagcg cctcgagcgc ctcgggctcg aagcccggct
    11221 cgacgtggac gacccgctcg acccggtgga ggcgcggccg gtcgaccgcg tcctgcgggg
    11281 gaaggccctc gtcgagcacg ccggccgtca cctgcgcgag cgcgctgcgg atgcgcgttc
    11341 cgcccgccga ccccgccgcg agcgcgacgc ccgccgcgtc gagcgcgacg agcggcgaca
    11401 tcatgctcgc catccgctcg cccggctcga gcctcgcgcc gagcaggtcc gactcgccga
    11461 gcatcgagtt caggtgcacc tggaactccg gcaggaagtc gcccgagccg agcccgaggc
    11521 tcgtcgtcag gacgcacacg ttcccggccg cgtcggcggt gacgaggttc gtcgtgtgcc
    11581 ccgagccgac gacgtccggg cccgagagcg cccgcgcgag cgcgagcacg cgccgcgcgg
    11641 gcggcgcgtc gcggaggcgc gggagcgacg agagcaccgg cgcgagcggc gtcagcgcat
    11701 cccgcgtgac gatccgggcc cccgcgtacg cgagctcggc gggctcgcgc cagaccggcc
    11761 ggtaggccgc gaggtcgtcg gcggtcacga ggccgttccg ctcgtcgcag agcgcgagga
    11821 gcgcgcgtcc gagcgtgccg tcgggccgga aggcaccggg accctcgccg gcgacggcct
    11881 cgagcgcgcg ggcgaggccc ggctggcgca gctcgtcgcc cgcgtccttg aggacgccgt
    11941 cctcccggag gaagatctcc gcgccctcgc gcatcgtcat gaccggcgcg agcatcgcga
    12001 ggcatgccgc gtgcgcgggc ggcagggcga ccccgtcgcg ggcgaacgcg atcgccgggg
    12061 cgcagagccg ctcccacgag agccgcccgt ggcgccgcca gagctcgtcc aggccggcca
    12121 cgacgccggg gacgccgcag gaggcgatcc cgatcgcgta gtggacgatc tccgagccga
    12181 acggcaccgc gagctcgacg acctcggcgt ccgggcgcgc gccgccgagg cccggcatcg
    12241 cgacgaagaa gtcgaggtgc tccgcgcggc ctcgccccgc gtcccaccag atcgcgtggc
    12301 cgccgccggc gagcccggtc atgaccacct cgacggcgca ggaggcgagc gacgcggcga
    12361 cggcggcgtc cgcggcagtc ccaccgtcgg cgagcgcctc gagcccgacg gccgcggtgg
    12421 cggggtgtcc ggcggcgatt ccgggcggga gggaggtggt cggcgtcatc gtgcgggatc
    12481 gtcgcagggt gggcggaaga ccgtctcggg ccgcgtgcta gcctaggcgc gatggctctg
    12541 acgaaggaca agaagcagga ggtcgtggga acgtacggcc gcagcgaggc cgacacgggc
    12601 tcgcctgagg tgcagatcgc gctcctcacg cgccggatcg aggagctcac cgagcacctc
    12661 cggacgcaca agaaggatca ccattcgcgt cgcgggctcc tgaagctcgt tggtcagcgg
    12721 cggcggctcc tgaactacct tcagaagaga aacctcgagg gctaccgcac gttgatcaag
    12781 gaactaggcc tgcgccgcta ataaaggcgc gcaggcgtcc gggagacgcc ggtggaagtt
    12841 tgagggagct cctccgacgg gagttggctc aaacctccgc ctcgtctccc ggtgagagag
    12901 gaggcacgat ggaaacgacg cttgccccgt ccgtggcgga tgtcacggtc acgatcggcg
    12961 acgccgagat ctcgttcgag acaggccgcc tggcgaagca ggccgacggc gcagtgctcg
    13021 tccgctcggg cgacacgatg gtcctcgcga ccgcgcaggg acggccggag gcgcgcgagg
    13081 gcgccgactt cttcccgctc accgtggacg tcgaggagcg gatgtacgcc gccgggaaga
    13141 tccccggcgg gttcttcaag cgggagggcc gcgcaagcga gcgcgccacg ctcaccgcgc
    13201 ggatgatcga ccgcccgatc cggccgctct ggccgaaggg cttccggaac gaggtgcagg
    13261 tcatctgcac cgtcctctcg gccgacctcg tgacgccgca cgacatcctc tgcctgaacg
    13321 gtgcgtcggc ggcgctcatg ctctcgccgc tcccgttcgc ggggccggtc ggcgcggtgc
    13381 gcgtcggcct ctccgagggc cggctcgtcg tcaacccgtc cctgcccgac ctcgaggccg
    13441 agagcgacct cgacctcatc gtcgtcggca cgaaggaggg cctcacgatg gtcgaggcgg
    13501 gcgcgaaccg ggtcgccgag gacacgctcc tcgacgcgct cgagctcgcg cacgaggaga
    13561 tcaggaagct ctgcgccgtc caggaggagc tccgcgagcg cgccggcaag gcgaagtggc
    13621 tcgacccggc cacgacggcc gagatcgagg agcagcacgc cgggcgcatc cgcgagcgga
    13681 tccagtcggc cggcctccgc gaggcggcgg ccgcatccga ggagatccag gccgacctcg
    13741 ccccgccgat cacgatggac tcgggcgagg aggaggcgat tcgcggcctc caggtgaaga
    13801 tgagcctcgc gctcgtcctc gagcgcgagc gcctcgaggc cgtcaagggc ccggtgcgcg
    13861 agcagttcga gaccgagctg cgcggcctca ccgacgccga gcaggactcg aagcagctca
    13921 agtccgcgaa gcgccagctt ctcttcgacc ggatcgtcga gaccgtggag ctcccgttcc
    13981 cggccggcgc ccccgtcgag ggcgacgggc cggcgctcaa ggacccgctc acgaaggggt
    14041 tcgtgaagaa ggccgccgag gcgatctaca aggagctcgt ccggacgaag atcgcggtcg
    14101 acaagcgccg ccccgacggg cgcggcgccg aggagatccg ctcgatcacg tgcgacgtcg
    14161 gcgtctcgcc gcgcacgcac ggctccgggc tcttcacccg cggccagacg cagatcatgt
    14221 cgctcctcac gctcggcacc gcgaaggagg ggcagcggat cgacgatctc tccctcgagg
    14281 aggagcgccg cttcatgcac cactacaact tcccgcccta ctccgtcggg gagaccgggt
    14341 tcatgcgggg tccgaagcgg cgcgacatcg gccacggcgc gctcgcgcag cgggcgctcg
    14401 agcccgtcat cccggggccg gaggagttcc cgtacacgat ccggatcgtc tccgagacgc
    14461 tcgagtcgaa cggctcgtcg tcgatgggct cggtctgcgg ctcgacgctc gcgctcatgg
    14521 acgccggcgt cccgatcgcg gagccggtca gcggcatcgc gatgggcctc gtgaaggagg
    14581 gcgacgacta cgtcatcctc accgacatcc agggcgcgga ggaccacctc ggcgacatgg
    14641 acttcaaggt ggccggcacg cgggacggga tcaccgcgct ccagatggac atcaagatca
    14701 ccggcgtcac gcaggagatc atgcgctccg cgctcgagca ggcgaagcgg gcccgcgtgg
    14761 cgatcatgga ctcgatgctc gaggcgatcc ccgaggcgcg caccgagctc gccgcgcacg
    14821 ccccgcgcat ctcgacgatg cagatcgacc ccgaccagat cgggctcgtg atcggcaagg
    14881 gcggcgagac gatccgctcg ctcgagtcgg actacgacgt ccagatcgac atcgaggagg
    14941 acggcacgct cctcatctac gccaccgagg ggacgaaggc cgacgaggcg atcggcgcga
    15001 tcaaggcgct cacgaaggag ccggagcccg gcgacgagta cacggggaag gtcgtgaaga
    15061 cgaccgactt cggcgcgttc gtcgagctga agaagggcac cgacgggctc ctccacgtct
    15121 cgaacgtcgg cccgggccgg gtcgcgcaca tcgaggacgt gatctcgcgc ggcgacgtcc
    15181 tcgacgtgaa ggtgctcgag gtcgacaagc agcggggccg gatcggcctc aagctcgtcg
    15241 cgaagcacga gaacggcgac ctcgtccagc cggagacgct gatcgagcgc gcgaaggacg
    15301 cgcccccgcg cgagcgggag gagcgcccgc cgcgcgaccg ggagcgcggc gggcggggcg
    15361 gccgcggccg cgaccggcgc tagaggacgt cgtgcgaggg gcggcgcgag ccgcccctcg
    15421 tcgttcgttc ccagtgggcg cgccgacggg tacggttccg cgatgactgg acgggtgacc
    15481 ggatgcccgc gccccgcttg gggcccgtac ggcgcggaga tactccgctg atcgtgacga
    15541 cgatccggcc atccggagcc gcccggtgag gcggtgggac gcgatcgtgg tcggcgcggg
    15601 gccggccggc tcgtcgacgg cgttccgcct cgcggaggcg ggcgcctccg tcctgctcct
    15661 cgaccgcgcg tcgttcccgc gggacaagcc ctgcgggggc ggcgtaacgc cgcgtgcgct
    15721 cgagcacgtc cccgtgccga tcgacccggt ggtcgagcac gtgacgaccg cggtcgagct
    15781 tcgcctcggc ttccgccgcg cggcggtccg cggccgggga ggccccctcg cgtacatgac
    15841 gcagcggcgg cgcctcgacg cgctcctcgc ggagcgcgcg gccggtgccg gggcggagct
    15901 ccgcgacggc gtgaaggtga cgggcgtcga ggcggggggc ggcggcgtcg tcgtgaccgc
    15961 gggcggcgag cggctgcacg gccggacggt cgtcggcgcc gacggggtga acggcgtcac
    16021 cgcccgg
//
LOCUS       DASTFC010000243         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1043849,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000243 DASTFC010000000
VERSION     DASTFC010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043849"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFO88_07305"
     CDS             <1..376
                     /locus_tag="VFO88_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545368.1"
                     /translation="ERLAAATAALARHVAPGGLLVVEPWILPEQWAPDHIGAVFVDEP
                     ELKVARINSHGGTPVADVLVMDLAYLVGTPGRVEHFTERHEVGMFTREQYLGAVEAAG
                     LAAEWIEGGLIGRGLALGTRGA"
     gene            432..1034
                     /gene="nth"
                     /locus_tag="VFO88_07310"
     CDS             432..1034
                     /gene="nth"
                     /locus_tag="VFO88_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9545369.1"
                     /translation="MIERLALEHPDAVIALRFLDDIELLVSVMLSAQTTDVTVNRVTE
                     SLFRKYRRPEDYLAVPLEELERDIRPTGTFRQKARNLRGAMRMLVEEYDGEVPTRLEE
                     LVRLPGVGRKTANVVAAERGATQGIVVDTHVRRLSQRLGFSRQGDPVKIERDLRRLVP
                     RADWARFPHLLIWHGRRVCIARAPRCADCVLNDLCPSSRV"
     gene            complement(1148..1969)
                     /locus_tag="VFO88_07315"
     CDS             complement(1148..1969)
                     /locus_tag="VFO88_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9545370.1"
                     /translation="MPTSALLLALGAAGLHAVWNLLLARARDPEAATAVALVVATVAF
                     APVAALTWDVEREAWPWIAASAGLELVYFVLLAGAYRRAEFGLVYPLARGLAPVLVLA
                     AALVVLGADPTLGQALGVGVIAAGVVLVRGVHGADARGTAFAVGIAACIAAYTLVDKE
                     GMRHAAPLPYLEIVLVGPALAYAGLIGSVKGRAALRAEAGWAPAVVGIIMFGSFALTL
                     AALGRAPAAPVAALRETGVVWAALLTIPFLQERMSPVRFAGAVLVAAGIAILALA"
     gene            2047..2373
                     /locus_tag="VFO88_07320"
     CDS             2047..2373
                     /locus_tag="VFO88_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545371.1"
                     /translation="MRAVDLAVFADALAGRAGALAARLERGRDQIRQAAIEREARRSL
                     GAETVARLVELGVLSRADVRGLRAEARRLAADLAAVEELQAWIEARLFAERERERESE
                     PARDAA"
     gene            complement(2380..2820)
                     /locus_tag="VFO88_07325"
     CDS             complement(2380..2820)
                     /locus_tag="VFO88_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9545372.1"
                     /translation="MPYFICPNCKRRSIDHDRRDGLTHQAVGCPSCGFGFLFELMDDY
                     YPGPSTGFVVCDQNRRVLAAGRGVFELTGFQERELLGREVEEALGLGVERPSPAEVAV
                     EWGVRQLGKELELQTKAGVRKAVKVDFFPAYDEDGGLLVALAPA"
     gene            complement(2877..>3889)
                     /gene="trxB"
                     /locus_tag="VFO88_07330"
     CDS             complement(2877..>3889)
                     /gene="trxB"
                     /locus_tag="VFO88_07330"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET9545373.1"
                     /translation="PAARKERVSENGIRKVIIIGGGPAGYTAALYSARANLEPLVIEG
                     FQWGGQLMITSDVENYPGYPDGVLGPQMMQDFRRQAERFGAEFVTDDVTRVDFSSQPL
                     RVWTGEKEHRAETVIVATGATARQLGLPSEQRLQGRGVTYCATCDAAFYKEKTVVVVG
                     GGDSAMEEALFLTRFASKVLVVHRRDAFRASPIMVDRVRANEAIELVLDSVVEEVLGD
                     EKVEAVRVRNVKTGETTDLATDGIFVAIGHDPTTALFRGQLDMDDAGYLLTDGKSTVT
                     KAPGVFAAGDVVDHVYRQAITAAGMGCQAALDAERFLAARDGGEPQPLVEEGALATPR
                     NW"
BASE COUNT          535 a         1435 c         1422 g          497 t
ORIGIN      
        1 ggagcggctc gcggccgcga cggcggcgct cgcgcgtcac gtcgcgccgg gcgggctcct
       61 cgtggtcgag ccgtggatcc tgccggagca gtgggctccc gaccacatcg gcgccgtctt
      121 cgtcgacgag ccggagctga aggtggcgcg gatcaactcg cacggcggga cgcccgtcgc
      181 ggacgtcctg gtcatggatc tcgcctacct cgtcggcacg ccggggcgcg tcgagcactt
      241 caccgagcgg cacgaggtcg gcatgttcac gcgcgagcag tacctcggcg cggtcgaggc
      301 ggccggcctc gcggcggaat ggatcgaggg cgggctgatc ggccgcgggc tcgcgctcgg
      361 gacgcgtggc gcgtagctac gtccacgagg ctcggcgccg cgtcggcccg aagcgggcgc
      421 ggatccgccc ggtgatcgag cggctggcgc tcgagcatcc cgacgcggtg atcgcgctcc
      481 gcttcctgga cgacatcgag ctgctcgtct ccgtgatgct ctccgcgcag acgaccgacg
      541 tcaccgtgaa ccgggtcacg gagtcgctct tccgcaagta ccggcgtccg gaggactacc
      601 tcgccgtgcc gctcgaggag ctcgagcgcg acatccgccc gaccgggacc ttccggcaga
      661 aggcccggaa cctgcggggc gcgatgcgga tgctcgtcga ggagtacgac ggggaggtgc
      721 cgacccggct cgaggagctc gtccggctcc ccggcgtcgg gcgaaagacg gcgaacgtcg
      781 tcgccgccga gcgcggcgcg acgcagggga tcgtcgtcga cacgcacgtc aggcggctct
      841 cgcagcggct cggcttcagt cggcaggggg acccggtgaa gatcgagcgc gacctgcgcc
      901 gcctcgtccc gcgcgcggac tgggcccggt tcccgcacct gctcatctgg cacggtcgcc
      961 gcgtctgcat cgcgcgggcc ccgcgctgcg ccgactgcgt cctgaacgac ctctgcccgt
     1021 cgagccgtgt ctagcgcgac cacggcttga acggccaccc ggcttcgggg caccccagcc
     1081 caccacccac ttccgagtct agtccggaaa accgcgcgca acgtgccgac ttcgtcacgg
     1141 atgtgtgtca ggcgagcgcg aggatcgcga tcccggccgc gacgagcacc gcgcccgcga
     1201 accgtaccgg cgacatgcgc tcctggagaa acgggatcgt gagcagcgcg gcccacacga
     1261 cgcccgtctc ccggagcgcc gcgaccggtg ccgccggcgc ccgcccgagc gcggcgagcg
     1321 tcagcgcgaa cgagccgaac atgatgatcc cgacgacggc gggcgcccat ccggcctcgg
     1381 cgcggagcgc ggcgcggccc ttgaccgagc cgatcagccc ggcgtacgcg agcgcgggcc
     1441 cgacgagcac gatctccagg tagggaagcg gcgccgcgtg ccgcatgccc tccttgtcca
     1501 cgagcgtgta cgcggcgatg caggcggcga tcccgaccgc gaacgccgtt ccgcgcgcgt
     1561 ccgccccgtg gacgccccgg acgaggacga ctcccgccgc gatcacgccc acgccgagcg
     1621 cctggccgag cgtcggatcc gcgccgagca cgacgagcgc ggccgcgagg acgagcacgg
     1681 gcgcgaggcc gcgcgcgagc gggtagacga gcccgaactc ggcccgccgg taggcgcccg
     1741 cgaggaggac gaagtacacg agctcgagcc cggcgctcgc ggcgatccac ggccacgctt
     1801 cccgctcgac gtcccaggtg agcgcggcga cgggtgcgaa ggcgaccgtc gcgacgacga
     1861 gcgcgaccgc cgtcgccgcc tcggggtcgc gcgcccgtgc gaggagcagg ttccagaccg
     1921 cgtgcagacc ggccgcgccg agcgcgagga ggagggcgga cgtcggcacg cgggcgagac
     1981 tacgtgacgg ttccgacacg cttccggcac gatgcgtgcg ctttcggcga gtagcgtcgt
     2041 gggcgtatgc gcgccgtcga cctcgccgtc ttcgcggacg cgctcgcggg ccgcgcgggc
     2101 gcgctcgccg cgcggctgga acgtgggcgc gaccagatcc ggcaggcggc gatcgagcgc
     2161 gaggcgcgcc ggtcgctcgg ggccgagacc gttgcgcggc tcgtcgagct cggcgtcctc
     2221 tcccgggcgg acgtgcgcgg gctgcgcgcc gaggcgcgca ggctcgcggc cgacctcgcc
     2281 gccgtcgagg agctccaggc gtggatcgag gcgcgcctct tcgccgagcg ggagcgcgaa
     2341 cgcgagtccg agcccgcgcg ggacgccgcg tgagcgcccc tacgccggcg cgagcgcgac
     2401 gaggaggccg ccgtcctcgt cgtaggcggg gaagaaatcc accttgaccg ccttccgaac
     2461 cccggccttc gtctggagct cgagctcctt gccgagctga cggacgcccc actcgaccgc
     2521 gacctcggca gggctcgggc gttcgacgcc caggccgagc gcctcctcga cctcccggcc
     2581 gagcagctcg cgctcctgga accccgtcag ctcgaagacg ccgcggccgg ccgccagcac
     2641 acgccggttc tggtcgcaga caacgaaccc ggtcgacggc ccggggtagt agtcgtccat
     2701 cagctcgaag aggaagccga agccgcagct cgggcacccc accgcctggt gcgtgaggcc
     2761 gtcgcgtcgg tcgtggtcga tcgaccggcg cttgcagttc ggacagatga agtagggcac
     2821 gacggcgatg ctagccacgc cccggcaggc cggcgcgggt cagccgccgc gtccggctac
     2881 cagttgcggg gtgtcgcgag cgcgccctct tcgacgagcg gctgcggctc gccgccgtcc
     2941 cgcgcggcga ggaagcgctc ggcgtcgaga gccgcctggc agcccatgcc ggcggcggtg
     3001 atcgcctgcc ggtacacgtg gtcgacgacg tccccggccg cgaacacgcc cggggccttc
     3061 gtcaccgtgg acttgccgtc ggtgaggagg tagccggcgt cgtccatgtc gagctgaccc
     3121 ctgaagagcg cggtcgtcgg atcgtggccg atcgccacga agatcccgtc cgtcgcgagg
     3181 tcggtcgtct cgcccgtctt cacgttgcgc acgcgcacgg cctcgacctt ctcgtcgccg
     3241 agcacctcct cgacgacgga gtcgagcacg agctcgatcg cctcgttcgc gcggacgcgg
     3301 tcgaccatga tcggcgacgc gcggaaggcg tcgcggcgat ggacgaccag caccttcgac
     3361 gcgaagcggg tgaggaagag cgcctcctcc atggcggagt cgcctccgcc cacgacgacc
     3421 accgtcttct ccttgtagaa cgccgcgtcg caggtcgcgc agtacgtgac cccgcgtccc
     3481 tggagccgct gctcggacgg aagcccgagc tggcgggcgg tggcgcccgt ggcgacgatc
     3541 accgtctcgg cgcggtgctc cttctcgccc gtccacacgc ggagcggctg cgaggagaag
     3601 tcgacgcgcg tgacgtcgtc ggtcacgaac tcggcgccga agcgctcggc ctgccggcgg
     3661 aagtcctgca tcatctgcgg cccgaggacg ccgtccgggt accccgggta gttctcgacg
     3721 tcgctcgtga tcatgagctg gccgccccac tggaacccct cgatcacgag cggctcgagg
     3781 ttcgcacgcg cgctgtagag cgcagcggtg tagccggcgg ggccgccgcc gatgatgatg
     3841 accttccgga tgccgttctc gctcacgcgt tcctttctcg ccgcagggc
//
LOCUS       DASTFC010000244         4029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_368285,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000244 DASTFC010000000
VERSION     DASTFC010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368285"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1121)
                     /locus_tag="VFO88_07335"
     CDS             complement(162..1121)
                     /locus_tag="VFO88_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET9545374.1"
                     /translation="MLNGLIDLLNDQTPLYPPWGRIVVVACLFALAWVVSRAAARVAH
                     QVLRWHDRRHSDDARDDLDERIASLKRHETAVGIVRTSITYVAFALAIVLSIAQVAGG
                     VDRLAAIAGGIFAVLVAAFVAQRLLVDLIAGFTMFLERWYSVGDTIVIVAAYELQGVV
                     EDVSLRRTRIRAVNGEVINVHNSAISAVRVLPSGVKELAMELYLSERAAGEDVVESVA
                     LMVPEGPTTFVRRPWIEQVEELSPDLVRMTVRATVAPGREWLAESLFPDLLRERAHDG
                     LIVHGPVTLAVDERALRTFARASATTRRNARRAELARIADATA"
     gene            complement(1231..1563)
                     /gene="trxA"
                     /locus_tag="VFO88_07340"
     CDS             complement(1231..1563)
                     /gene="trxA"
                     /locus_tag="VFO88_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9545375.1"
                     /translation="MAEMLQLTDANFEQEVLQADTPVVVDFWAPWCGPCRMIAPILDE
                     MATEHEGRVRFAKLNVDDNPQTAARFNVLSIPMVILFEGGEPRETVIGARPKSHYEGA
                     WQQWLTAA"
     gene            complement(1597..2322)
                     /locus_tag="VFO88_07345"
     CDS             complement(1597..2322)
                     /locus_tag="VFO88_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET9545376.1"
                     /translation="MSLVICLPTYDERDNLEAMVRALGDVFERAGLDGRVLVVDDASP
                     DGTGEIADRLAAELPWVGVLHRERKEGLGPAYLAGFRRALADGAELVMEMDCDFSHDP
                     QDVPRLVRGTEEADVVVGSRYAPGGGVRNWGLLRRAISRGGCLYAQAILGFGVRDSTA
                     GFKVFRREVLETIDLDAIRSKGYAFQIENVYRALRAGFRVAEVPIVFADRETGSSKMS
                     RRIVLEAVVKVPALRLAALRGRL"
     gene            2309..>4029
                     /gene="polX"
                     /locus_tag="VFO88_07350"
     CDS             2309..>4029
                     /gene="polX"
                     /locus_tag="VFO88_07350"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET9545377.1"
                     /translation="MTRLTAHIIAVVAGEAGKGAETRALPSNAELAERFDLLADLLEL
                     DGADAFRLSAYRRASARIRESAAPVARLALEGTATQIPGVGGTIEAKIVELSETGDLA
                     ALRKLRDRVPAGLVEIMRVPGLGPKTARRLWQELGVATLDDLREAAESEKLRELSGLG
                     PKTEERVLAALAKPAAATATDTGRALLGKVLPVVRGLVEELAAHPACDRVSEAGSIRR
                     RFETAKDVDLIATSEDPAALTAWFVAHPRVAEVAAHGPSKATVVTYDGYRFDLRVVPP
                     ESYGSLLQHFTGSKDHNVAIREDAVRRGLSVSEYGVQDVETGETDTAAEETEVYERLG
                     YAWIPPELRENRGELEAARAGTLPELVTLEDVRGDLHMHTTWSDGRATLDEMVAAAKE
                     EGREYVAICDHAKRLKGDLLARQGEAIATVNESLKGIEVLRGVEVDIRRDGSLDMADD
                     LLAELDWVMASIHSGFSGSRDELTKRLVAAMENPHVDCIGHPTGRKINKRAPYELDLD
                     AVFAASVETGTFLEVNSQPDRLDLRDVHARAAAEAGVGIVVSTDAHRLHELANLELGV
                     FQARRGWL"
BASE COUNT          591 a         1475 c         1457 g          506 t
ORIGIN      
        1 gccgaggcgg ggcgcgtcga gatcggctac gcggacgtgc cgaccggcgg gcggctcacc
       61 taccgtgccg acgagggcgt gctcgtgacc gccctccacg cctggttcga cgcgcagctc
      121 atggatcacg gcgcggacgc cgaggccggc gggcccgaag gctaggccgt cgcgtccgcg
      181 atgcgggcga gctcggcccg ccgggcgttc cggcgcgtcg tcgcgctcgc gcgggcgaaa
      241 gtccgaagcg cccgctcgtc cactgcgagc gtgaccggcc cgtggacgat cagcccgtcg
      301 tgcgcccgct cgcgcaggag atccgggaac aggctctccg cgagccactc gcggcccggc
      361 gcgaccgtcg cgcgcaccgt catccggacg agatcgggcg agagctcctc gacctgctcg
      421 atccagggcc ggcgcacgaa cgtcgtcggc ccctcgggca ccatgagcgc gaccgactcg
      481 accacgtcct ccccggccgc gcgctcgctg aggtagagct ccatggcgag ctccttgacg
      541 ccggacggca gcacgcgcac cgccgagatc gcggagttgt ggacgttgat cacctcgccg
      601 ttcacggcgc ggatgcgcgt ccggcggagc gacacgtcct cgacgacgcc ctggagctcg
      661 tacgccgcga cgatcacgat cgtgtccccg acggagtacc accgctcgag gaacatcgtg
      721 aacccggcga tcaggtcgac gaggagccgc tgcgcgacga acgcggcgac gagcaccgcg
      781 aagatccctc ccgcgatcgc cgcgagccgg tcgacgccgc ccgcgacctg cgcgatcgag
      841 aggacgatcg cgagcgcgaa cgcgacgtac gtgatcgacg tgcggacgat gccgaccgcc
      901 gtctcgtgcc gcttgagcga ggcgatccgc tcgtcgaggt cgtccctcgc gtcgtccgag
      961 tgccgccggt cgtgccaccg gaggacctgg tgggccaccc gcgcggccgc gcgtgacacg
     1021 acccacgcga gcgcgaagag acacgcgacg acgacgatcc tcccccacgg cgggtagagg
     1081 ggcgtctggt cgttcaggag gtcgatcagg ccgttgagca tgcggaagga ggcggcgtca
     1141 cgagccgtcc gcaatcgaag gtacagacag ccgttcggcc ctcacaaggc cctcctccgg
     1201 gccctaaccc gcgttagccg gttcttaacg tcaggcggcg gtcagccact gctgccacgc
     1261 gccctcgtag tggctcttcg ggcgggcgcc gatcaccgtc tcgcggggct cgcctccctc
     1321 gaagaggatc accatcggga tcgagaggac gttgaagcgg gccgccgtct gcgggttgtc
     1381 gtcgacgttc agcttcgcga agcggacgcg gccctcgtgc tcggtcgcca tctcgtcgag
     1441 gatcggcgcg atcatccggc acggcccgca ccagggggcc cagaagtcga ccacgacggg
     1501 cgtgtccgcc tggaggacct cctgctcgaa gttcgcgtcg gtcagctgga gcatctcggc
     1561 cacgcggcga ctatacccgc ggggacgggc gccgcatcag agccgcccgc gcagagccgc
     1621 gagccggagc gccggcacct tcacgaccgc ctcgaggacg atccgccggc tcatcttcga
     1681 gctgccggtc tcgcggtcgg cgaagacgat cggcacctcg gcgacccgga acccggcccg
     1741 gagcgcccgg tagacgttct cgatctggaa cgcgtagccc ttcgagcgaa tcgcgtccag
     1801 gtcgatcgtc tcgagcacct cccgccggaa gaccttgaac cccgcggtcg agtcgcggac
     1861 gccgaagccg aggatcgcct gcgcgtagag gcagccgccg cggctgatcg cccgccgcag
     1921 gagcccccag ttccgcacgc cgccgccggg cgcgtagcgg gagccgacca cgacgtccgc
     1981 ctcctccgtt ccgcggacga ggcgcgggac gtcctgcggg tcgtgcgaga agtcgcagtc
     2041 catctccatg acgagctcgg cgccgtcggc gagcgcgcgg cggaaccctg cgaggtacgc
     2101 gggcccgagc ccctccttgc gctctcggtg gaggacgccg acccagggga gctcggccgc
     2161 gagtcggtcg gcgatctcgc cggtcccgtc cggcgaggcg tcgtcgacga cgagcacgcg
     2221 cccgtcgagg ccagcgcgct cgaagacgtc cccgagcgcc cgcaccatcg cctcgaggtt
     2281 gtcgcgctcg tcgtacgtcg gaaggcagat gacgaggctc acggcgcata tcatcgccgt
     2341 cgtggcgggc gaggccggga agggagcgga gacgagggcg ctgccgtcga acgcggagct
     2401 cgccgagcgc ttcgacctgc tcgccgacct gctcgagctc gacggcgcgg acgcgttccg
     2461 gctctccgcc taccgccgcg cctcggcgcg catccgcgag tcggccgccc cggtggcgcg
     2521 gctcgcgctc gaagggacgg cgacgcagat ccccggggtc ggcgggacga tcgaggcgaa
     2581 gatcgtcgag ctctccgaga cgggcgacct cgcggcgctc cggaagctcc gcgaccgcgt
     2641 ccccgctggg ctcgtcgaga tcatgcgcgt ccccggcctc gggccgaaga ccgcgcgccg
     2701 gctctggcag gagctcggcg tggcgacgct cgacgacctc cgggaggcgg ccgagtcgga
     2761 gaagctccgt gagctctccg gcctggggcc gaagacggag gagcgcgtgc tcgccgcgct
     2821 cgcgaagccg gccgccgcga cggcgacgga caccgggcgg gcgctcctcg gcaaggtgct
     2881 cccggtcgtg cgcgggctcg tggaggagct cgccgcccac ccggcgtgcg accgcgtgtc
     2941 ggaggcgggc tcgatccggc gccggttcga gaccgcgaag gacgtcgacc tcatcgccac
     3001 ctcggaggac ccggccgccc tgaccgcgtg gttcgtcgcc cacccgcgcg tcgccgaggt
     3061 cgcggcgcac gggccgtcga aggcgacggt cgtgacgtac gacggctacc gcttcgacct
     3121 gcgcgtcgtc ccgcccgagt cttacgggag cctcctccag cacttcaccg gctcgaagga
     3181 tcacaacgtc gcgatccgcg aggacgcggt gcggcggggg ctctccgtct ccgagtacgg
     3241 cgtgcaggac gtcgagaccg gggagaccga caccgccgcc gaggagacgg aggtctacga
     3301 gcggctcggc tacgcctgga tcccgccgga gctgcgcgag aaccgcggcg agctcgaggc
     3361 cgcgcgcgcc ggcacgctgc cggagctcgt cacgctcgag gacgtccgcg gcgacctcca
     3421 catgcacacg acgtggtcgg acgggcgcgc gacgctcgac gagatggtgg cggcggcgaa
     3481 ggaggagggc cgcgagtacg tggcgatctg cgaccacgcg aagcggctga agggcgacct
     3541 gctcgcgcgc cagggcgagg cgatcgcaac cgtgaacgag agcctgaaag ggatcgaggt
     3601 gctgcgcggc gtcgaggtcg acatccgtcg cgacgggtcg ctcgacatgg cggacgacct
     3661 cctcgcggag ctcgactggg tgatggcgtc gatccactcg ggcttcagcg gctcgcgcga
     3721 cgagctgacc aagcgcctcg tcgccgcgat ggagaacccg cacgtcgact gcatcggaca
     3781 tccgaccgga cggaagatca acaagcgcgc cccgtacgag ctcgacctcg acgcggtctt
     3841 cgcggcctcc gtcgagacgg gcacgttcct cgaggtcaac tcccagcccg accggctcga
     3901 cctccgcgac gtccacgcgc gcgccgccgc cgaggccggc gtcgggatcg tcgtctcgac
     3961 ggacgcgcac cgcctgcacg agctcgcgaa cctcgagctc ggggtcttcc aggcgcgccg
     4021 cggctggct
//
LOCUS       DASTFC010000245         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_187230,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000245 DASTFC010000000
VERSION     DASTFC010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187230"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFO88_07355"
     CDS             <1..468
                     /locus_tag="VFO88_07355"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET9545378.1"
                     /translation="QQVEIEDLQRSVDRRANRIEALERAIRRDEIRLASDALTPEERL
                     RVELRIERLRDGLGYARRERAALLREAASAELTVDLHTREAKPGAAAPQGRIERAARD
                     GLAALAVAASVGVFVIVLGGPLLLLAALAWGLRRRRVRRANEALLDRPGAAAS"
     gene            504..692
                     /locus_tag="VFO88_07360"
     CDS             504..692
                     /locus_tag="VFO88_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545379.1"
                     /translation="MDEERKEGRPGELEETIDLDLPQDPGIPGREEEGRAAEADRTLA
                     PGSMPPPSERDRDDDDAA"
     gene            complement(689..1420)
                     /locus_tag="VFO88_07365"
     CDS             complement(689..1420)
                     /locus_tag="VFO88_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9545380.1"
                     /translation="MVRIAAVGDVHAAPEKREALRTAFASLDGVDLVLVAGDLTTHGE
                     PEQAAVMADAAAGSTAPVVTVLGNHDLHVDRADELVAVLEDAGIVVLDRATRTFDVRG
                     TSVGVVGTKGFVGGFPDSQLPDFGEALLRRVYAETTEEVLALERGLEEIRGCDVRIVL
                     LHYAPTASTLAGEPETIWTFLGSDRLAVPIAAHPPHIVLHGHGHAGTFRGAIGDVPVY
                     NVAQPVIKRDFWIFELDGEAPAGRG"
     gene            1643..2209
                     /locus_tag="VFO88_07370"
     CDS             1643..2209
                     /locus_tag="VFO88_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET9545381.1"
                     /translation="MSDFEDICETMKKAAAALRESGVPFLLAGGLAAWARGGPSSDHD
                     VDFLVRPEDVDEAADTLAAAGFRIERPPEGWLVKAYDGDILVDLIFEPTTGPVTEELI
                     ARGEEREVLATPMRVMALEDVMVTKLLALGETHLDYAGPVEIARAVREQVDWDEVRGR
                     TDDSPYARAFFALVEELGVVEPNGTGIG"
     gene            2347..>3479
                     /locus_tag="VFO88_07375"
     CDS             2347..>3479
                     /locus_tag="VFO88_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9545382.1"
                     /translation="MERYDPQAIERKWQETWRRERAFHVPNPGEPGADGAREHSYVLE
                     MLPYPSGELHMGHVRNYMLGEIVTHFRRRNGMAVLRPMGFDAFGLPAENAAIREGGHP
                     REVTERNIASIRRQMERMGWAIDWDRVFATHEPEYYRWTQWLFLRFFERGLAYRKEAP
                     VKWCPNDQTVLANEQVIDGRCERCGAEVEAKSLAQWFFRITDYADVLLDEMALLEDWP
                     ERVLTMQRNWIGRSEGAELRFRVDGSPDLEVPVFTTRPDTLFGATFFVLAPEHPLVPE
                     LVAERPEQADVLEYVRRSAARSAVEREEKEKDGVFTGRHVVNPATGEPIPVWIADYVL
                     MEYGTGAIMGVPAHDERDFAFAERYDLPIRTVVAPADGAEGPVA"
BASE COUNT          486 a         1258 c         1298 g          437 t
ORIGIN      
        1 cagcaggtcg agatcgagga cctccagcgc tccgtcgacc gccgcgcgaa ccggatcgag
       61 gcgctcgagc gcgcgatccg ccgggacgag atccgcctgg cgagcgacgc gctcacgccc
      121 gaggagcggc tgcgcgtcga gctgcggatc gagcggctcc gcgacgggct cgggtacgcg
      181 cgccgcgagc gcgccgcgct cctccgggaa gcggcgtccg cggagctcac cgtcgacctc
      241 cacacccgcg aggcgaagcc gggcgccgcg gcgccgcagg gccggatcga gcgggccgcg
      301 cgggacgggc tcgccgcgct cgcggtcgcc gcctccgtcg gcgtcttcgt gatcgtcctc
      361 ggcgggccgc tcctcctgct ggccgcgctc gcgtgggggc tgcgccggcg acgggtgcgc
      421 cgcgcgaacg aggcgctgct cgaccgcccc ggcgcggcgg cgtcgtaggg ttgccgcgcg
      481 ggacggtcgg gtaggcgccc gccatggacg aggaacgcaa ggaaggccgg ccaggcgagc
      541 tcgaggagac gatcgacctc gacctcccgc aggatccggg catcccgggc cgcgaagagg
      601 aaggacgggc cgccgaggcc gaccggacgc tcgcgccagg ctcgatgccg ccgccgagcg
      661 agcgcgaccg ggacgacgac gacgcggcct agccgcgtcc cgccggcgcc tcgccgtcga
      721 gctcgaagat ccagaagtcc cgcttgatca ccggctgcgc gacgttgtag acggggacgt
      781 cgccgatcgc gccgcggaac gtccctgcgt ggccgtgccc gtgcaggacg atgtgcggcg
      841 ggtgcgcggc gatcggcacg gcgaggcggt cgctgccgag gaacgtccag atcgtctccg
      901 gctcgcccgc gagcgtcgag gcggtcggcg cgtagtggag gaggacgatg cggacgtcgc
      961 agccgcgaat ctcctcgagc ccgcgctcga gggcgagcac ctcctcggtc gtctcggcgt
     1021 agacgcggcg gaggagggcc tcgccgaagt cggggagctg cgagtcgggg aacccgccca
     1081 cgaacccctt cgttccgacg acgccgaccg aggtgccgcg gacgtcgaac gtacgggtcg
     1141 cgcggtcgag cacgacgatc ccggcgtcct cgaggacggc gacgagctcg tccgcgcggt
     1201 cgacgtggag gtcgtggttc ccgagcacgg tcacgaccgg ggccgtcgat cccgccgccg
     1261 cgtcggccat gaccgccgcc tgctccggct cgccgtgcgt cgtcaggtcg cccgcgacga
     1321 gcacgaggtc gacgccgtcg agcgaggcga acgcggtgcg gagcgcctcg cgcttctccg
     1381 gcgcggcgtg cacgtccccg acggcggcga tgcgaaccat caccgcggct cgtttcccgt
     1441 gcgggcggac tcgatgcctc gcggaccgcg ccccgacctc gtcggtccac ggccccccaa
     1501 cgggggagcg agcgtccctt cggtcacgct agcgggaaag ccgtcacgtg tcagtgccgg
     1561 aaccgtgccg aaacgcgcac gagcctgcgc ccgctccggc ggcgcggttc ggcggcgccg
     1621 ctcgcggggt acgcaccgcg ccgtgagcga cttcgaggac atctgcgaga cgatgaagaa
     1681 ggccgccgcg gcgcttcgcg agtcgggcgt gccgttcctc ctcgcgggcg ggctcgccgc
     1741 ctgggcgcgc gggggcccgt cctccgacca cgacgtcgac ttcctcgtcc gcccggagga
     1801 cgtcgacgag gcggccgaca cgctcgccgc cgccggcttc cggatcgagc gcccgcccga
     1861 gggctggctc gtgaaggcgt acgacggcga catcctcgtc gacctcatct tcgagccgac
     1921 caccggcccc gtgaccgagg agctcatcgc gcgcggcgag gagcgggagg tgctcgcgac
     1981 gccgatgcgc gtgatggcgc tcgaggacgt catggtgacg aagctcctcg cgctcggcga
     2041 gacgcacctc gactacgccg gcccggtcga gatcgcgcgt gccgtccgcg agcaggtcga
     2101 ctgggacgag gtgcgcggcc gcaccgacga ctccccgtac gcgcgcgcgt tcttcgcgct
     2161 cgtcgaggag ctcggcgtcg tcgagccgaa cggcacgggg atcggctagg ctgcgtccga
     2221 atggagaacg tcctccttat cgacggacag gggtagcggc cggccgaacc ggacgccgtc
     2281 acgcgaaccc ccgaagcctc tcgcccgaga ggctttttcg tctccggagg cgaacagaag
     2341 gacgcgatgg agcggtacga cccacaagcg atcgagcgga agtggcagga gacgtggcgg
     2401 cgcgagcgtg ccttccacgt cccgaaccct ggcgagccgg gcgccgacgg ggcgcgcgag
     2461 cactcgtacg tcctcgagat gctcccgtat ccgtcgggcg agctccacat ggggcacgtc
     2521 cgcaactaca tgctcggcga gatcgtcacg cacttccggc gccggaacgg gatggctgtg
     2581 ctgcgcccga tgggcttcga cgcgttcggg ctcccggccg agaacgccgc gatccgcgag
     2641 ggcggccacc cgcgcgaggt gaccgagcgg aacatcgcgt cgatccgccg ccagatggag
     2701 cggatgggct gggcgatcga ctgggaccgc gtcttcgcga cgcacgagcc ggagtactac
     2761 cgctggacgc agtggctctt cctccgcttc ttcgagcgcg ggctcgcgta ccgcaaggag
     2821 gcgccggtca agtggtgccc gaacgaccag accgtcctcg cgaacgagca ggtgatcgac
     2881 ggccgctgcg agcgctgtgg cgccgaggtg gaggcgaaga gcctcgcgca gtggttcttc
     2941 cggatcacgg actacgccga cgtcctgctc gacgagatgg cgctcctcga ggactggccc
     3001 gagcgcgtgc tcacgatgca gcggaactgg atcgggcgct cggagggcgc cgagctccgg
     3061 ttccgcgtgg acggctcgcc cgacctcgag gtgcccgtgt tcacgaccag gccggacacg
     3121 ctcttcggcg cgacgttctt cgtcctcgcg cccgagcatc cgctcgtgcc ggagctcgtc
     3181 gcggagcggc ccgagcaggc cgacgtcctc gagtacgtgc gccgctccgc cgcccgctcg
     3241 gccgtcgagc gcgaggagaa ggagaaggac ggcgtcttca ccggccgcca cgtcgtgaac
     3301 ccggcgaccg gcgagccgat ccccgtctgg atcgcggact acgtcctcat ggagtacggg
     3361 accggcgcga tcatgggcgt ccccgcgcac gacgagcgcg acttcgcgtt cgccgagcgc
     3421 tacgaccttc cgatccgcac ggtcgtcgcg ccggcggacg gcgccgaggg cccggtcgc
//
LOCUS       DASTFC010000246         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1587720,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000246 DASTFC010000000
VERSION     DASTFC010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587720"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFO88_07380"
     CDS             <1..298
                     /locus_tag="VFO88_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HET9545383.1"
                     /translation="VAHVTGGGIPGNLARVLPSGLGAVIEPDAWERPPVFRWLAGQGV
                     AEDEQRRVFNLGIGYCAIVHPGDAGNTVLQANAGAGIIGRVAAGVDGVVFADAG"
     gene            438..995
                     /gene="purN"
                     /locus_tag="VFO88_07385"
     CDS             438..995
                     /gene="purN"
                     /locus_tag="VFO88_07385"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET9545384.1"
                     /translation="MIGVLVSGEGTNLQALLDAELPVAAVASNRPGVRALERAASAGV
                     PGAVFPLGDYASRAERDAAMAEWLAGRGARLVVCAGYMHLLTPGFLERFPVLNVHPSL
                     LPAFPGMHAVEDALAAGVSETGVTVHFVDEGIDTGPVVLQERVPVEPGDTAESLHARL
                     HEVEHRLLPQAARLYLEGGIRSPES"
     gene            1005..2546
                     /gene="purH"
                     /locus_tag="VFO88_07390"
     CDS             1005..2546
                     /gene="purH"
                     /locus_tag="VFO88_07390"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET9545385.1"
                     /translation="MVARALISVFDKTGLDDFARDLHELGVELVASGGTATFIAELGL
                     PVTRVDELTEMPEMLSGRVKTLHPAIHAAILARRDLETDIAALERHGIEPFDLVCVNL
                     YPFQQVAARKDVREEDAVEMIDIGGPTMLRAAAKNFVHVLPVCEAERYGTIVAELRES
                     GDIGIQTRRALAAEAFSHTAAYETSIATWFADRESFPDRLLLSLEKVIDLPYGENPHQ
                     RAAYYAEFGARRHLLSRVDQLGGKQLSFNNLNDLDAARSLVREFTVPACAIVKHANPC
                     GCALAADVEDAYAAALAADPVSAYGGVVALNRKVEAPLAERIAAQFTEVLIAPEYSPE
                     AIEILRRKEGTRILQDRERRGPGPGERNLRRVLGGFLVQDADLEHEDRDRMETPTSRS
                     PNEAIWGDLLFAWRVAKHVASNAIVLAKGLQTIGIGTGQPSRVDAVRIALEKAREHGH
                     DPTGSVLASDAFFPFPDGPELALDAGVMAIIQPGGSKRDDEVAAAVDRARAVMVLTGR
                     RHFRH"
BASE COUNT          364 a         1014 c          988 g          347 t
ORIGIN      
        1 ggtcgcccac gtgaccggcg gcggaattcc cgggaacctc gcgcgcgtgc tgccgtccgg
       61 gctcggcgcg gtgatcgagc cggacgcgtg ggagcgcccg cccgtcttcc gctggctcgc
      121 cggccagggg gtcgccgagg acgagcagcg ccgcgtcttc aacctcggga tcgggtactg
      181 cgcgatcgtc caccccgggg acgctggtaa cacagtgtta caagccaacg cgggcgccgg
      241 gatcatcggg cgcgtcgcgg cgggcgtcga cggcgtcgtc ttcgccgacg ccgggtgagc
      301 cggacccgtc cgagccgcgc acgtccccgg cggacacgac cggtgcctgg caccggacaa
      361 tgtcccgccg ggacacggcc ggtgcctggc acgggacaat ggcccggcgg gacgcggccg
      421 gaggggccag gggcgcggtg atcggcgtcc tcgtctccgg cgaggggacg aacctccagg
      481 cgctgctcga cgcggagctc ccggtcgccg cggtcgcctc gaaccggccc ggcgtgcggg
      541 cgctcgagcg cgccgcgagc gcgggcgtcc ccggcgccgt cttcccgctc ggcgactacg
      601 cctcgcgggc ggagcgcgac gccgccatgg ccgagtggct cgccgggcgc ggcgcccggc
      661 tcgtcgtctg cgcggggtac atgcacctcc tcacacccgg cttcctcgag cggttcccgg
      721 tcctgaacgt ccatccgtcg ctcctgccgg cgttccccgg gatgcacgcc gtcgaggacg
      781 cgctcgccgc cggcgtctcg gagaccggcg tcaccgtgca cttcgtcgac gaggggatcg
      841 acacggggcc ggtcgtgctc caggagcgcg tgccggtcga gcccggcgac acggccgagt
      901 cgctccacgc ccggctgcac gaggtcgagc accgcctcct cccgcaggcg gctcgcctct
      961 atctggaagg cgggatacgc tcccccgaga gctgaccccg ccgcatggtc gcccgcgcac
     1021 tcatctccgt cttcgacaag acaggtctcg acgacttcgc ccgggacctg cacgagctcg
     1081 gcgtcgagct cgtcgcgagc ggcggcacgg cgacgttcat cgcggagctc ggcctcccgg
     1141 tcacgcgcgt ggacgagctg accgagatgc cggagatgct cagcgggcgg gtgaagacac
     1201 tccacccggc gatccacgcg gcgatcctcg cgcgccgcga cctcgagacc gacatcgcgg
     1261 cgctcgaacg gcacgggatc gagccgttcg acctcgtgtg cgtgaacctc tacccgttcc
     1321 agcaggtcgc ggccaggaag gacgtccgcg aggaggacgc ggtcgagatg atcgacatcg
     1381 gcggcccgac catgctccgc gcggcggcga agaacttcgt ccacgtcctg cccgtctgcg
     1441 aggccgagcg ctacgggacg atcgtcgccg agctgcgcga gagcggcgac atcgggatcc
     1501 agacccgccg tgcgctcgcg gccgaggcgt tctcccacac ggccgcgtac gagacgtcga
     1561 tcgcgacgtg gttcgcggac cgcgagtcgt tccccgaccg gctgctcctc tcgctcgaga
     1621 aggtgatcga cctcccgtac ggcgagaacc cgcaccagcg cgccgcctac tacgcggagt
     1681 tcggcgcgcg gcggcacctg ctctcgcgcg tcgaccagct cggcgggaag cagctctcct
     1741 tcaacaacct gaacgacctg gacgcggcgc gctcgctcgt gcgcgagttc acggtcccgg
     1801 cctgcgcgat cgtgaagcac gcgaacccgt gcggctgcgc gctcgcggcc gacgtcgagg
     1861 acgcgtacgc ggcggcgctc gcggccgacc cggtctcggc ctacggcggg gtcgtcgcgc
     1921 tgaaccggaa ggtcgaggcg ccgctcgccg agcggatcgc ggcgcagttc accgaggtgc
     1981 tgatcgcgcc cgagtactcg ccggaggcga tcgagatcct acgccgcaag gaggggacgc
     2041 ggatcctcca ggaccgggag cgccgcgggc ccggcccggg cgagcgcaac ctgcggcgcg
     2101 tgctcggcgg gttcctcgtc caggacgccg acctcgagca cgaggaccgc gaccggatgg
     2161 agactcccac ctcccgctcg ccgaacgagg cgatctgggg cgatctcctc ttcgcctggc
     2221 gcgtggcgaa gcacgtggcc tcgaacgcga tcgtcctcgc gaaggggctc cagacgatcg
     2281 ggatcggaac gggccagccg agccgcgtgg acgccgtgcg gatcgcgctc gagaaggcgc
     2341 gcgagcacgg tcacgacccg accggctccg tcctcgcctc cgatgcgttc ttcccgttcc
     2401 cggacgggcc ggagctcgcg ctcgacgcgg gcgtcatggc gatcatccag ccgggcggct
     2461 cgaagcgcga cgacgaggtc gccgcggcgg tcgaccgggc gcgggccgtg atggtgctca
     2521 ccggccgccg tcacttccgc cactagcagc ccgcgggggc ccgagcgcgg gcgacgcgcg
     2581 ggaaggaccg cggcgctcgc gggcgagggc ggacgccgcc gattaggctt ccgcccgtgc
     2641 cgcgcgacta cccgacgatc ctcgacctcg tgggcggcac accgctcgtc cgcttccagc
     2701 ggatcaacgc ggg
//
LOCUS       DASTFC010000247         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1604318,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000247 DASTFC010000000
VERSION     DASTFC010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604318"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFO88_07395"
     CDS             <1..816
                     /locus_tag="VFO88_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9545386.1"
                     /translation="ELVEARLGELAFSPDLGALEEALRYSLLAGGKRIRPVLCLATGE
                     ALGCRPDELVDAACALELVHTFSLVHDDLPALDDDDLRRGRPSAHVRFGEDVAILAGD
                     ALLTEAFRLALAYPAPDVARVLADATLGMIGGQYLDVTTNGGLDADGLAELHALKTGR
                     LIRASVLAAAAVAGLGEAERERWAALGAELGLLFQIVDDLLDATATTEELGKTAGKDE
                     AAGKVTYVSLHGLERARALADETRARVEERLAALPVDTTVLAELAGSIRDRRA"
     gene            816..1523
                     /locus_tag="VFO88_07400"
     CDS             816..1523
                     /locus_tag="VFO88_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HET9545387.1"
                     /translation="MLHRRAGDELVVVGQPAHALVAGALARAWAGPFAPRDAVCLAAE
                     QHDVGWTGWELAPELDPATGLPYTFADLPVPRRLALWAGAGERLVPQSRYAALLVSLH
                     GTLLVERFPPPPEHAAIVQAYLARERAFQETLRGSLGADPVELARNRELVFTWDALSL
                     ELVHGVSGSRAVAGFTLSANGPDVRVAPWPFREAALAVSFEGRVLGGTFTDEAAMRAA
                     LDAAPWVVVETPLRPAT"
     gene            complement(1516..1875)
                     /locus_tag="VFO88_07405"
     CDS             complement(1516..1875)
                     /locus_tag="VFO88_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9545388.1"
                     /translation="MLLLVRIAVWWATNLAALWVAAELVEGIAYDDFWALALAALVFA
                     AVNLVLRPLVILLTLPAVLLTFGILLLFINAFMVWLTGQIVPGFEVDDFFWAAVLGAL
                     VVWVVNFVLDRVFDAFT"
     gene            1964..>2470
                     /locus_tag="VFO88_07410"
     CDS             1964..>2470
                     /locus_tag="VFO88_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET9545389.1"
                     /translation="MKKRLDVLLVERGLAESRAQAQALVLAGRVKGHEKAGEQVDEAA
                     PLEVAPGPRFVSRGGEKLANALDALGLDVAGEDCLDVGASTGGFTDCLLQAGAARVCA
                     LDVGYGQLHPKLRDDPRVTVLERTNVRRLDCAALPFAPTFVVCDVSFIGLDKALPAAL
                     RCAAPGWRA"
BASE COUNT          317 a          925 c          918 g          310 t
ORIGIN      
        1 gagctcgtcg aggcgcggct cggggagctc gcgttctcgc ccgatctcgg cgcgctcgag
       61 gaggcgctcc ggtactcgct cctcgcgggc gggaagcgga tccggccggt gctctgcctc
      121 gccacggggg aggcgctcgg ctgccggccg gacgagctcg tggacgccgc ctgcgcgctc
      181 gagctcgtgc acacgttctc gctcgtgcac gacgacctgc ccgccctcga cgacgacgat
      241 ctgcggcgcg gccggccgag cgcacacgtc cgcttcggcg aggacgtcgc gatcctcgcg
      301 ggggatgcgc tcctcacgga ggccttccgg cttgccctcg cgtaccctgc gcccgacgtc
      361 gcccgcgtgc tcgccgacgc gacgctcggg atgatcggcg ggcagtacct cgacgtgacg
      421 acgaacggcg gcctcgacgc cgacgggctc gccgagctcc acgctctcaa gaccgggcgc
      481 ctgatccgcg cgtccgtcct ggccgccgcg gctgtggccg gcctcgggga ggccgagcgc
      541 gagcggtggg ccgcactcgg cgcggagctc gggctcctct tccagatcgt cgacgacctc
      601 ctggacgcga ccgcgacgac ggaggagctc gggaagaccg ccgggaagga cgaggcggcc
      661 ggaaaggtca cgtacgtctc gctccacggg ctcgagcgcg cgcgtgcgct cgccgacgag
      721 acgcgggcgc gggtcgagga gcggctcgcc gcgctcccgg tcgacacgac cgtcctcgcg
      781 gagctcgccg gctcgatccg cgaccggcgc gcctgatgct ccaccggcgg gccggcgacg
      841 agctcgtcgt cgtcgggcag cccgcgcacg cgctcgtcgc cggcgcgctc gcccgcgcgt
      901 gggcgggccc gttcgcgccg cgcgacgccg tctgcctcgc ggccgagcag cacgacgtcg
      961 gctggaccgg gtgggagctc gcgccggaac tcgacccggc gaccgggctt ccgtacacgt
     1021 tcgccgacct gccggtgccg cgccggcttg cgctctgggc cggcgccggc gagcggctcg
     1081 tgccgcagtc gcgctacgcg gcgctcctcg tctcgctcca cgggacgctc ctcgtcgagc
     1141 gcttcccgcc gcccccggag cacgcggcga tcgtccaggc gtacctcgcg cgcgagcggg
     1201 ccttccagga gaccctgcgc gggtcgctcg gcgccgaccc cgtcgagctc gctcggaacc
     1261 gggagctcgt cttcacgtgg gacgcgctct cgctcgagct cgtacacggc gtctccggtt
     1321 cgcgggccgt cgccggcttc acgctctcgg ccaacgggcc ggacgtgcgc gtcgcaccat
     1381 ggccgttccg cgaggcggcg ctcgccgtct cgttcgaggg ccgcgtgctc ggagggacgt
     1441 tcacggacga ggcggcgatg cgcgccgcgc tcgacgccgc gccctgggtc gtcgtcgaga
     1501 cgccgctccg tccggctacg tgaacgcgtc gaagacccgg tcgagcacga agttcacgac
     1561 ccagacgacg agcgctccga ggacggcggc ccagaagaag tcgtccacct cgaagcccgg
     1621 gacgatctgg ccggtcagcc agaccatgaa cgcgttgatg aagaggagca ggatcccgaa
     1681 cgtgagcagg acggccggga gcgtcagcag gatgacgagc ggccgcagca cgaggttgac
     1741 ggccgcgaag acgagcgccg cgagcgcgag cgcccagaag tcgtcgtacg cgatgccctc
     1801 cacgagctcg gccgcgaccc agagggcggc gaggttcgtc gcccaccaga cggcgatccg
     1861 gacgaggagg agcactgcgc gaatctacaa gaaagcggaa aacctcgtcc acagccgcac
     1921 gcgcgggcag ggaaccggcg gggcaggcga ctaagctggc tccgtgaaga agcggctgga
     1981 cgtcctcctc gtcgagcgcg gcctcgcgga gagccgcgcg caggcgcagg cgctcgtcct
     2041 cgccggacgc gtgaagggcc acgagaaggc gggcgagcag gtcgacgagg cggcgccgct
     2101 cgaggtcgcg ccgggcccgc gcttcgtctc gcgcgggggc gagaagctcg cgaacgcgct
     2161 cgacgcgctc ggcctcgacg tggccgggga ggactgcctc gacgtgggcg cctcgaccgg
     2221 cggcttcacc gactgccttc tccaggcggg cgcggcccgg gtctgcgcgc tcgacgtcgg
     2281 ctacggccag ctccacccga agctccgcga cgacccgcgt gtgaccgtgc tcgagcggac
     2341 gaacgtccgc cgcctcgact gcgccgcgct tccgttcgcg cccaccttcg tcgtctgcga
     2401 cgtctcgttc atcgggctcg acaaggcgct gccggccgcg ctgcgctgcg cggcgccggg
     2461 ctggcgcgcg
//
LOCUS       DASTFC010000248         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_253017,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000248 DASTFC010000000
VERSION     DASTFC010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253017"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1110)
                     /gene="dapE"
                     /locus_tag="VFO88_07415"
     CDS             complement(22..1110)
                     /gene="dapE"
                     /locus_tag="VFO88_07415"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET9545390.1"
                     /translation="MPAPAGDASRLAATTLALVDIPSESRNEAAAAAWVEDAVPVGRL
                     ALVHAADSTLLYLTERRAGRPLVLLAGHLDTVPAQENIPGQIGGGAVHGLGASDMKGG
                     VAVMVELARWLEEEGAPLAVDLALLFFPREELPPDESALPALFAAYPPVLEAELGIML
                     EPTDCAIHAGCLGNLVADLVWHGVSAHSARPWTGTNAVERAVAGLADVVPWPAREIEL
                     SGLRFVEVASVTGIEGGIAMNVVPDRCTARVNLRYAPDRSAGSAEELVRELAERAAAD
                     EVAVVGNSPSAPVVVETPLVDRLRSAGDLDVLPKQAWTPVAELAEAGVDAVNFGPGAT
                     AQAHRREEHVEIAALERVFRVLRDFLRD"
     gene            1212..2468
                     /gene="hflX"
                     /locus_tag="VFO88_07420"
     CDS             1212..2468
                     /gene="hflX"
                     /locus_tag="VFO88_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9545391.1"
                     /translation="MLVAAPGSDPADELAELAELCRTAGVEPAGELVQFRTAPDPRTY
                     VGKGKLAELKEAVQASGAQIVIVDDDLDPSQQRRIEEALGLGVLDRTQLILDIFAQHA
                     VSAEGKLQVELAQLEYNLPRMRGMWLHLERLGGGAGAGRRGPGESQLETDRRLARRRI
                     TVLKERLRGLEKRRETQRKARREAATPLVALAGYTNAGKSTLLNALTDANVSVNDRLF
                     ETLDPTTRAFEEDGRRYLLTDTVGFIRRLPHGLVEGFAATLEETLLSDLVLHVVDASA
                     TDEHIAETIASVESVLAEIGAAHLPRELVLNKVDAVDPLGRRRLANRFPDAVQVSART
                     GEGLDRLKARVAERLGDRFEPVRLLVPYSEGRVLADLYELGAPIEERADRPDGVHVLA
                     RLPRGELGRYAPYLVVEEDARAKHGA"
     gene            2458..2925
                     /gene="dut"
                     /locus_tag="VFO88_07425"
     CDS             2458..2925
                     /gene="dut"
                     /locus_tag="VFO88_07425"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET9545392.1"
                     /translation="MAPDDGGGRLELPVRRLRDDAVLPEQAYAGDAGLDLATCERVEL
                     GPGERALVPTGLAVAVPDGYAGFVQPRSGLAARRGITVVNSPGLIDSGYRGEVKVVLL
                     NTDPVESFVAEPGERIAQLVVLRVPAVEPVEVDALPESERGALGFGSSSLAGR"
     gene            2922..>3256
                     /locus_tag="VFO88_07430"
     CDS             2922..>3256
                     /locus_tag="VFO88_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9545393.1"
                     /translation="MSAEPRIRVSALLRREGRILLCRHEKPGREYWLLPGGGVNLGES
                     LADALQRELTEEVGIAEDLPLEGPVAVCDSIAPDGTPVRKHVVHIIFAGYLGGRSLEA
                     VTSQDAAVRG"
BASE COUNT          415 a         1205 c         1246 g          390 t
ORIGIN      
        1 acgggggaga ggcgcacggc gtcagtctcg caggaagtcg cgcagcaccc ggaagacgcg
       61 ctcgagggcg gcgatctcga cgtgctcctc gcgccggtgc gcctgcgcgg tcgcgcccgg
      121 cccgaagttg accgcgtcca cgcccgcctc ggccagctcg gcgaccggcg tccacgcctg
      181 cttcgggagc acgtcgagat ccccggcgga gcgcagtcga tcgacgagcg gcgtctcgac
      241 gacgacgggc gccgacggcg agttcccgac gaccgcgacc tcgtccgcgg ctgctcgctc
      301 cgcgagctcg cgcacgagct cctccgccga cccggccgag cggtcgggcg cgtagcggag
      361 gttcacgcgc gccgtgcagc ggtcggggac gacgttcatc gcgatcccgc cctcgatccc
      421 ggtcacgctc gcgacctcga cgaagcggag gccggagagc tcgatctcgc gggcgggcca
      481 cggcacgacg tcggcgagcc cggcgaccgc gcgctcgacc gcgttcgtcc ccgtccacgg
      541 ccgcgcggag tgcgcgctca cgccgtgcca gacgaggtcg gcgacgaggt tgccgaggca
      601 gcccgcgtgg atcgcgcagt cggtcggctc gagcatgatc ccgagctccg cctcgagcac
      661 cggcgggtag gcggcgaaga gcgccgggag cgcgctctcg tcgggcggaa gctcctcgcg
      721 cgggaagaag aggagcgcga ggtcgaccgc gagcggcgcg ccctcctcct cgagccagcg
      781 cgcgagctcg accatgacgg cgacgccgcc cttcatgtcg ctcgcgccga gcccgtgcac
      841 ggcgccgcct ccgatctggc cggggatgtt ctcctgcgcg ggaacggtgt cgaggtgccc
      901 cgcgaggagg acgagcggcc ggcccgcgcg acgttcggtc aggtagagga gggtcgagtc
      961 ggccgcgtgc acgagcgcga gccgcccgac cggcaccgcg tcctcgaccc aggccgccgc
     1021 cgcggcctcg ttccggctct ccgacgggat gtccacgagg gcgagcgtcg tcgcggccag
     1081 gcgggacgcg tctccggcgg gggcgggcac cgcggcagcc tatccgggcc ggaggcgggc
     1141 ataatccgtg ccggatgcct caacgccgac ctgatcccgc gccaggcgca gcgcccgagc
     1201 gcgggttcgc cgtgctggtc gccgcgcccg gatcagaccc cgccgacgag ctcgccgagc
     1261 tcgccgagct gtgccggacc gccggcgtcg agccggccgg cgagctcgtc cagttccgca
     1321 cggcgccgga tccgcgcacg tacgtcggca agggcaagct cgccgagctg aaggaggccg
     1381 ttcaggcgtc gggcgcgcag atcgtgatcg tcgacgacga cctcgacccg agccagcagc
     1441 ggcggatcga ggaggcgctc gggctcggcg tcctcgaccg gacgcagctc atcctcgaca
     1501 tcttcgccca gcacgccgtc tcggccgagg gaaagctcca ggtcgagctc gcgcagctcg
     1561 agtacaacct cccgcggatg cgcggcatgt ggctccacct cgagcgcctc ggcggcgggg
     1621 cgggggcggg gcgacgcggc cccggcgagt cgcagctcga gaccgaccgc cggctggcgc
     1681 ggcggcgcat caccgtcctg aaggagcggc tccgcgggct cgagaagcgg cgcgagacgc
     1741 agcggaaggc gcgccgcgag gccgcgacgc cgctcgtcgc gctcgcgggc tacacgaacg
     1801 cgggcaagtc gacgctcctg aacgcgctca cggacgcgaa cgtctccgtg aacgaccgcc
     1861 tcttcgagac gctcgacccc acgacccgcg cgttcgagga ggacgggcgg cgctacctcc
     1921 tgaccgacac ggtcggcttc atccgccggc tgccgcacgg gctggtcgag ggcttcgcgg
     1981 cgacgctcga ggagacgctc ctctccgacc tcgtcctgca cgtcgtggac gcgtccgcca
     2041 cggacgagca catcgccgag acgatcgcct ccgtcgagtc cgtgctcgcg gagatcgggg
     2101 cggcgcacct cccgcgcgag ctcgtcctga acaaggtgga cgcggtcgac ccgctcgggc
     2161 ggcggcggct cgcgaaccgg ttcccggacg cggtgcaggt ctccgcccgg accggggagg
     2221 ggctcgaccg gctgaaggcg cgcgtggccg agcggctcgg cgaccgtttc gagccggtgc
     2281 ggctcctcgt cccgtactcc gagggccgcg tcctcgccga cctctacgag ctcggcgcgc
     2341 cgatcgagga gcgggccgac cggccggacg gcgtccacgt gctcgcgcgg ctgccccgcg
     2401 gcgagctcgg ccgctacgcg ccgtatctcg tcgtcgaaga ggacgcgcgc gcgaagcatg
     2461 gcgcctgacg acggcggcgg gcggctcgag cttccggtcc ggcggctgcg cgacgacgcc
     2521 gtgcttccgg agcaggcgta cgcgggcgac gcggggctcg acctcgcgac ctgcgagcgc
     2581 gtggagctcg ggccgggcga gcgggcgctc gtcccgacgg gcctcgcggt cgccgtcccg
     2641 gacgggtacg cggggttcgt gcagccgcgc tccgggctcg cggcgcgccg ggggatcacg
     2701 gtggtgaact cgccggggct catcgactcc ggctaccgcg gcgaggtcaa ggtggtgctc
     2761 ctgaacaccg accccgtcga gagcttcgtg gccgagccgg gggagcggat cgcgcagctc
     2821 gtcgtcctcc gagtgcccgc ggtcgagccc gtcgaggtgg acgcgctgcc ggagagcgag
     2881 cgcggcgccc tgggcttcgg ctcgtcgagc ctggcgggcc ggtgagcgcg gagccgcgga
     2941 tccgggtgtc ggccctcctg cggcgtgagg ggcgcatcct cctctgccgg cacgagaagc
     3001 ccgggcgcga gtactggctc ctgcccggcg gcggcgtgaa cctgggcgag agcctcgccg
     3061 acgcgctcca gcgcgagctg accgaggaag tcgggatcgc ggaggatctc ccgctcgagg
     3121 gcccggtcgc ggtgtgcgac tcgatcgcgc ccgacgggac gcccgtccgg aagcacgtcg
     3181 tccacatcat cttcgcgggc tacctcggcg gccggtcgct cgaggcggtc acgtcgcagg
     3241 acgccgccgt gcgcgg
//
LOCUS       DASTFC010000249         5897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_71757,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000249 DASTFC010000000
VERSION     DASTFC010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71757"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFO88_07435"
     CDS             <1..225
                     /locus_tag="VFO88_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9545394.1"
                     /translation="AHYARRFEGRIYRYAERHRVKSGITGWAQVNGLRGKTSLADRVE
                     WDNHYIENWSLWFDVQILVRTLAAVVRSPS"
     gene            239..1690
                     /locus_tag="VFO88_07440"
     CDS             239..1690
                     /locus_tag="VFO88_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HET9545395.1"
                     /translation="MSSFDDAPARGLVLRLAANSLAQAGGSFLAAVVGFLTFVAVTRG
                     LGPAAYGHLTAATAFLFIPTVLAEAGLSAALLRQISARPEETEHALRASLPLRALIAL
                     VALIAALAIALVSPLPYLTKVAIAISAAGSLCTLLTQSLMPVLQAQLKMHLSVAATLA
                     GRLLTLGLTLGVLAAGLGFKSVVAAQVAGAVLVLVLHAGIVGRLVPLRPLVDTAYWRR
                     LVTGSLALGLAIALSQAYFRIDAVLLALLRDAYEVGLYGAAYKFIEISELLVVAIPIS
                     VFPPLARFVATGDPRATVLVQRAFDVMLAAAAPLAVGMIVFAEPVVVAAAGEEFREGA
                     DALRLLGPFVLFSFVNGLLFRILMAHGRDKLLLATTGTVLVANVVLNLVFIPVYGFRA
                     AAVVSVVTEMLVLVPLAVAARRRGLAPRLGYVPVVAAAAGLMAGVGLLVPGPALIAMA
                     ASSVVYLGTVLLLPGTARDVAFRHLLPWLRRGE"
     gene            complement(1673..3334)
                     /locus_tag="VFO88_07445"
     CDS             complement(1673..3334)
                     /locus_tag="VFO88_07445"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9545396.1"
                     /translation="MAEAVMPRETSSTHRRTPLARRAVLTTLLAGLALLAAPGALASA
                     EGTEPLESTIFRPVADTYVDAARPRAAHGMRKRLRASATRKRTYLRFRVGGFAEIQDA
                     MLYVRVARGRSVRFNVRRARERRWSELRMTFRSAPRAERGSPRAVGVARPGWAAVDVS
                     AIVRANGPVTLVLGPRALRSPLAFKSRESSAPAQLLVRGKKGKGPQAPTEPAPTEPAP
                     SGNPYADRVGFSGSNVWYDQTKQLNYMTKLRSAGVTWLREDFHWGAFEPSPGTWNWTY
                     GDRLMRNASLTGLNILGIFAYSSSWGADGATIYHPPKDPSAYANFARRVVERYGPNGT
                     FWQENPTLAPRPLTAVEIWNEPWLSFFWRPNPEPARYAQLVRAASAAIKAAQPTVKVL
                     ASADIFQMRSDTTQSRDWFRLVLEADPTLFRDAVDVLSVHTYSQARSPLDTVTAPRWR
                     FDRFRLTRDLAAAYGANKPIWITEFGWTTEPGNADAVNEATQATYTQQALDLALRQWG
                     SVVEKAFVYYWGESSDSYTAGYSPLRPDGSAKPLWNTVQALLAAP"
     gene            3562..5088
                     /locus_tag="VFO88_07450"
     CDS             3562..5088
                     /locus_tag="VFO88_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545397.1"
                     /translation="MRPSAAEALKAAIRRSLPLTIALILLGTVTVSAIRQLNGPQYEA
                     TARVVHSADLGPIVSGVQMPYTDPQRVIDTATELANTPELYERASESGDVGSSQTLQD
                     SVEVSGGADTDVLTFTCTREEATLAVDCVNVVAAEYVEWRAELSGEAVGRAIEQLEEQ
                     IDDLPASGGGVTTQERAELRDQLNRLRVLDTLNTGGATLIESAVVAEKTSPRPIRDAV
                     FGGALGLVIALLIAGIREAFNTRIRSEADVEDALGRPVLAAIQSLPKRTKLVTVGRHE
                     SRWGDTYALLAANIMQIRGKADRTIIAVTSAIAGEGKTTTATNLAVAMAQRGQRVILV
                     DFDLRKPSVGRVFRIPQGSPGVVQVVDGTAELDRTLWSIPLNGKSGGQAVAATNGHGD
                     LGGLREDGKGSLRVIAAGGHERGARIARSARVRELLDRLANDADVVVLDTPPALATVE
                     MAELSRSVDLVVGVVRYGRVTRRSLIALNRQAETWQTDLVGAVITDAPPEEDDYYYYK
                     "
     gene            5101..>5897
                     /locus_tag="VFO88_07455"
     CDS             5101..>5897
                     /locus_tag="VFO88_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545398.1"
                     /translation="MNAVLRSGFFRGDLAHFALGAFVLFMTLAASTRLGEVLSVTLLV
                     GLAVLGLVVIAFVGVPHVAVAATIPLFAFLPMVKALWVPWVGPLKDVVTVAAALAALV
                     VVVLAARGGRRIPGDLWIVALIGFLLALYALNIGGDLERSWAWGHGLRLFALPLALLL
                     AGLTLPNPRRTLHWALVSLIATCTVVALVGIAQQLAGPARLVEFGWLWNRNVRTIDGF
                     LRSFGTLDEPFAYAALLMFGLAAILMWAPRRPIVFAAAGIIAVGLSLS"
BASE COUNT          723 a         2175 c         2104 g          895 t
ORIGIN      
        1 gcgcactacg cgaggcggtt cgagggcagg atctaccgtt acgctgagcg ccaccgcgtg
       61 aagtccggga tcaccggctg ggcgcaggtc aacgggctgc gcggcaagac ctcgctcgcc
      121 gaccgcgtcg aatgggacaa ccactacatc gagaactggt cgctctggtt cgacgtccag
      181 atcctcgtcc gcacgctcgc cgccgtggtg cgcagcccct cgtagccggc ccgcgcgcgt
      241 gagctccttc gacgacgccc ccgcgcgggg cctcgtcctc cgcctcgcgg cgaactcgct
      301 cgcccaggcc ggcgggagct tcctcgccgc cgtcgtcggc ttcctcacct tcgtcgcggt
      361 cacgcgcggg ctcgggccgg ccgcctacgg ccacctgacg gcggcgacgg cgttcctctt
      421 catccccacc gtgctcgccg aggcggggct ctcggccgca ctcctgcgcc agatctcggc
      481 gcggccggag gagacggagc acgccctgcg cgcctcgctg ccgctccgcg ccctgatcgc
      541 gctcgtcgcc ctgatcgccg cactcgcgat cgcgctcgtc tcgccgctgc cctacctcac
      601 gaaggtcgcg atcgcgatct cggcggcggg ctcgctctgc acgctcctga cgcagagcct
      661 catgcccgtc ctccaggcgc agctcaagat gcacctctcg gtcgcggcga cgctcgcggg
      721 gcggctcctg acgctcgggc tcacgctcgg cgtgctcgcg gccgggctcg ggttcaagag
      781 cgtcgtcgcg gcgcaggtgg cgggcgccgt gctcgtgctg gtgctccacg ccggcatcgt
      841 cgggaggctc gtgccgctgc ggccgctcgt cgacacggcc tactggcgga ggctcgtgac
      901 cggctcgctc gccctcgggc tcgcgatcgc gctctcccag gcgtacttcc gcatcgacgc
      961 cgtcctcctc gcgctcctcc gcgacgcgta cgaggtcggg ctctacggcg ccgcgtacaa
     1021 gttcatcgag atctcggagc tgctcgtcgt cgcgatcccg atctcggtct tcccgccgct
     1081 cgcgcgcttc gtggcgaccg gcgacccgcg cgcgaccgtc ctcgtccagc gggcgttcga
     1141 cgtcatgctc gcggccgccg ccccgctcgc ggtcgggatg atcgtcttcg ccgagccggt
     1201 cgtcgtcgcc gcggccggcg aggagttccg cgagggcgcc gacgcgctgc gcctgctcgg
     1261 gccgttcgtg ctcttctcgt tcgtgaacgg cctcctcttc cggatcctca tggcgcacgg
     1321 gcgcgacaag ctgctcctcg ccacgacggg gacggtgctc gtcgcgaacg tcgtcctgaa
     1381 cctcgtcttc atcccggtgt acggcttccg ggccgcggcg gtcgtgtcgg tcgtgacgga
     1441 gatgctcgtg ctcgtgccgc tggccgtcgc cgcgcggcgc agggggctcg cgccgcgcct
     1501 cggctacgtg ccggtcgtcg cggcggcggc cgggctcatg gccggcgtgg ggctcctcgt
     1561 ccccgggccc gcgctcattg cgatggcggc gtcgtccgtc gtgtacctcg gcaccgtcct
     1621 cctcctcccg ggcacggctc gcgacgtcgc cttccggcac ctgctgccgt ggctacggcg
     1681 cggcgagtag ggcctggacg gtgttccaga gcggcttcgc gctcccgtcg ggcctgagcg
     1741 ggctgtaccc cgccgtgtac gagtcgctcg actcgcccca gtagtagacg aacgccttct
     1801 cgaccaccga gccccactgg cggagagcga ggtcgagcgc ctgctgcgtg taggtcgcct
     1861 gcgtggcctc gttgaccgcg tccgcgttcc cgggctcggt cgtccagccg aactcggtga
     1921 tccagatcgg cttgttcgcg ccgtaggcgg cggcgaggtc ccgcgtgagg cggaaccggt
     1981 cgaagcgcca gcgcggcgcg gtgaccgtgt cgagcgggct gcgggcctgc gagtacgtgt
     2041 gcaccgagag gacgtcgacc gcgtcgcgga agagcgtcgg gtcggcctcg agcacgaggc
     2101 ggaaccagtc gcgcgactgg gtcgtgtcgc tccgcatctg gaagatgtcc gcggaggcga
     2161 gcaccttcac ggtcggctgc gcggccttga tcgcggccga cgcggctcgg acgagctgcg
     2221 cgtagcgggc gggctccggg ttcggccgcc agaaaaagga gagccacggc tcgttccaga
     2281 tctcgaccgc cgtgagcggg cgcggggcga gggtcgggtt ctcctgccag aaggtgccgt
     2341 tcgggccgta gcgctcgacg acgcggcggg cgaagttcgc gtacgcgctc ggatccttgg
     2401 gcggatggta gatcgtggcc ccgtccgcgc cccagctcga ggagtacgcg aagatcccga
     2461 ggatgttcag gccggtcagc gaggcgttcc gcatgaggcg gtcgccgtac gtccagttcc
     2521 aggtgccggg cgagggctcg aaggcgcccc agtggaagtc ctcgcgcagc cacgtgacgc
     2581 cggccgagcg gagcttcgtc atgtagttga gctgcttcgt ctggtcgtac cagacgttcg
     2641 agccggagaa gccgacgcgg tcggcgtacg ggtttccgga cggcgcaggc tcggtcggcg
     2701 cgggctcggt cggcgcctgc gggcccttcc ccttcttccc gcgcacgaga agctgtgcgg
     2761 gtgcggacga ctcgcggctc ttgaacgcga gcgggctgcg cagggcgcgc ggcccgagga
     2821 cgagcgtcac cggcccgttc gcgcgcacga tggccgagac gtccacggcc gcccagcccg
     2881 ggcgggcgac gccgacggct cgcggcgacc cccgctcggc gcgcggggcg gagcggaacg
     2941 tcatgcggag ctccgaccag cggcgctcgc gcgcgcggcg gacgttgaac ctgaccgagc
     3001 gcccgcgggc gacgcgcacg tagagcatcg cgtcctggat ctccgcgaac ccgccgacgc
     3061 ggaagcggag gtacgtgcgc ttgcgcgtgg cggaggcgcg aagccgcttc cgcatcccgt
     3121 gcgccgcgcg cggccgcgcc gcgtcgacgt acgtgtcggc gaccgggcgg aagatcgtgg
     3181 actcgagcgg ctccgtgccc tccgccgacg cgagcgcgcc cggtgccgcg aggagcgcga
     3241 gccccgcgag cagggtcgtg aggacggccc ggcgggcaag cggcgtgcgg cggtgggtgc
     3301 tgctcgtctc cctcggcatg acggcttccg ccacgtggat cggcgcgcct ccgaggcgga
     3361 ggaaccctga ggggagggaa cggagtcccc cctttggagg taaaggcggc tacgacgttc
     3421 gcccctcgaa cgagggagaa cgcgccctcc gatgcttcaa tcgcgaccgc tacggctatc
     3481 gtggagagaa gtgagacacg acacgaaccc gtttgcggcc cggggtcatg gtgcgagccc
     3541 ttcgcgccga tactgcttcc gatgagaccg agcgctgcgg aagctctcaa ggctgcgatc
     3601 cgccgatcgc tgccgctcac gatcgccctg atcctcctgg gcaccgtgac cgtgagcgcg
     3661 atccgacagc tcaacgggcc gcagtacgag gcgacggctc gcgtcgtgca ctccgccgat
     3721 ctcggcccga tcgtctcggg cgtccagatg ccgtacaccg atccgcagcg cgtgatcgac
     3781 acggcgacgg agctcgctaa caccccggag ctctacgagc gcgcctcgga gagcggcgac
     3841 gtcggcagct cgcagacgct ccaggactcc gtcgaggtga gcggcggcgc ggacaccgac
     3901 gtcctcacct tcacgtgcac ccgcgaggaa gccacgctcg ccgtcgactg cgtgaacgtc
     3961 gtcgcggccg agtacgtcga gtggcgcgcc gagctctcgg gcgaggccgt cggccgcgcg
     4021 atcgagcagc tcgaggagca gatcgacgat ctgccggcga gcggcggcgg cgtcacgacg
     4081 caggagcgtg ccgagctgcg cgaccagctc aaccggctcc gcgtcctcga caccctgaac
     4141 accggcggcg cgacgctcat cgagagcgcc gtcgtcgccg agaagacgag ccccaggccg
     4201 atccgcgacg cggtcttcgg cggcgcgctc ggcctcgtga tcgccctcct catcgcgggc
     4261 atccgcgagg cgttcaacac caggatccgc tcggaggcgg acgtggagga cgccctcggg
     4321 cggcccgtcc ttgcggcgat ccagtcgctt ccgaagcgca cgaagctcgt caccgtcggt
     4381 cggcacgagt cccgctgggg cgacacgtac gccctgctcg cggcgaacat catgcagatc
     4441 cgagggaagg ccgaccgcac gatcatcgcg gtcacgagcg cgatcgcggg cgaggggaag
     4501 acgaccaccg cgacgaacct cgcggtcgcg atggcccagc gcggccagcg cgtcatcctc
     4561 gtcgacttcg acctgcgcaa gccgtcggtc gggcgcgtgt tccggatccc gcaggggtcg
     4621 ccgggcgtcg tccaggtggt cgacgggacg gccgagctcg accgcaccct ctggtcgatc
     4681 ccgctgaacg gcaagtcggg cggccaggcg gtcgccgcga cgaacggcca cggtgacctc
     4741 ggcgggctcc gcgaggacgg gaagggctcg ctgcgcgtca tcgccgccgg cgggcacgag
     4801 cgcggcgctc gcatcgcgcg ctccgctcgg gtcagggagc tcctcgaccg gctcgcgaac
     4861 gacgcggacg tcgtcgtgct cgacacgccg cccgcgctcg ccacggtcga gatggcggag
     4921 ctctcgcgga gcgtcgacct cgtcgtcggc gtcgtccgct acggccgcgt cacgcgccgg
     4981 agcctcatcg cgctcaaccg ccaggccgag acgtggcaga cggacctcgt cggcgccgtg
     5041 atcaccgacg ctccgccgga agaggacgac tactactact acaagtaggc ggccgcgggg
     5101 atgaacgcgg tgctgagaag cggcttcttc cgaggcgacc tggcgcactt cgcgctcggc
     5161 gccttcgtcc tcttcatgac gctcgccgcc tcgacgcggc tcggcgaggt gctctcggtg
     5221 acgctcctcg tcgggctcgc cgtcctcggc ctcgtcgtga tcgccttcgt cggggtgccc
     5281 catgtggcgg tcgcggcgac catcccgctc ttcgccttcc tcccgatggt caaggcgctc
     5341 tgggtcccct gggtcgggcc cctcaaggac gtcgtcaccg tcgccgcggc gctggccgcg
     5401 ctcgtcgtcg tcgtcctcgc cgcgcgcggc gggcgacgga tcccgggcga cctctggatc
     5461 gtcgccctca tcggcttcct cctcgccctc tacgcgctga acatcggcgg cgacctcgag
     5521 cggagctggg cgtggggcca cggcctccgg ctcttcgccc tgccgttggc gctcctgctc
     5581 gccgggctca cgctgccgaa cccgcgccgg acgctccact gggcgctcgt ctcgctcatc
     5641 gcgacctgca cggtggtcgc gctcgtcggg atcgcccagc agctcgccgg gcccgcgcgc
     5701 ctcgtcgagt tcggctggct ctggaaccgg aacgtccgga cgatcgacgg cttcctgcgc
     5761 agcttcggga cgctcgacga gccgttcgcc tacgccgcgc tcctcatgtt cgggctcgcc
     5821 gcgatcctca tgtgggcccc gcgccggccg atcgtgttcg ccgccgccgg gatcatcgcg
     5881 gtcggcctct cgctctc
//
LOCUS       DASTFC010000250         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1192500,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000250 DASTFC010000000
VERSION     DASTFC010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192500"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VFO88_07460"
     CDS             <1..1314
                     /locus_tag="VFO88_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9545399.1"
                     /translation="VDLLEEVERANLFVVALDDDRTWYRYHQLFADLLRAELLRHERG
                     RVPELHRRAFEWYRAAGLVDEAVDHALAVGELEAAGDAIAERWTPALAIGEARATLRR
                     LEALPADAVARDARLSLAHAWAASLTDRPAEARRALDAAREAGLRGAFPDGSSLDAVA
                     ALVEAFAVRGDVGALTAASRRACDLEAELGLAARPLARLSLGWAQSLAGAAEARAVLE
                     DAASSAAELGQPLHVSIAHALLARLALAEGDGDLAEAHARDAVAALARGEGDVLACGM
                     ANVALGSALARSAVAEAEALLERGLAALRVLGEPLAVADALLALAPVRRSRQGLPEAR
                     ACIAEARELVEAAADAGLLAGRLEEVARSLTPAYRKANGDSDLTEREVEVLRYLADGM
                     AKRDIATTLFLSYNTIHSHTKSIYHKLRVSSREDAVQKARELGAL"
     gene            1384..2709
                     /locus_tag="VFO88_07465"
     CDS             1384..2709
                     /locus_tag="VFO88_07465"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET9545400.1"
                     /translation="MAGNDAAKATGGERSHRILVGVLVVVGALLVLVSTVSVWIRDAA
                     LDTDVWVDQSGQLLESPDVRDALAVYIVDQAYSAGDVQQRLEEGLPEELRPLSGPIAA
                     QLQGVAYDAAARALARPRVQELWRTANGAAHERIVAVLEGESERVTLENGNVVLELDE
                     LVADVAARVGAGEGPTAALQGRVEPIVLVESEELDAAQTSVRVVKALSIWPFLLGLAL
                     WTGAVYLAHGRRRETVRAIAVSLVVLGVILLVVRRIGGNVIVESLVEAESVKPAAAVF
                     WSTFTTLLRESAIAGVVIGVLGLVWTWLSGPTRPATAIREWLAPTFRDRVALVHGTLA
                     AVILLLLLWGPVGSPRRLVSLVLVTGFAFLGLELLRRQAVREFPNAVRGAVSARAIFP
                     RLPFGRAEATAGEPVAATKVDRLERLAALHERGALTDEEYQAEKQLLLA"
     gene            complement(2731..>3076)
                     /locus_tag="VFO88_07470"
     CDS             complement(2731..>3076)
                     /locus_tag="VFO88_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545401.1"
                     /translation="GVGVAVVAAVFLSNMPEAIAATTGLAASGWTRGRILGLWSVVAL
                     VGGLASLLGYAAFGDASPRTLAFVLSFAGGAILTMLADTMMPEAFEHGGKRVGLATTL
                     GFGLALALTSLE"
BASE COUNT          362 a         1142 c         1162 g          410 t
ORIGIN      
        1 gtcgacctgc tcgaggaggt cgagcgggcg aacctcttcg tcgtcgcgct cgacgacgac
       61 cggacgtggt accgctacca ccagctcttc gccgacctgc tgcgggccga gctgctccgg
      121 cacgagcgcg ggcgcgtccc cgagctgcac cggcgggcct tcgagtggta ccgagccgcg
      181 gggctcgtcg acgaggccgt cgaccacgcg ctcgccgtgg gcgagctcga ggccgcgggc
      241 gacgcgatcg cggagcggtg gacgccggcg ctggcgatcg gcgaggcccg ggcgacgctt
      301 cgacggctcg aggcgcttcc ggccgacgcc gtcgcacgcg atgcgcggct ctcgctcgcg
      361 catgcctggg cggcgagcct caccgaccgc cccgccgagg cacgccgtgc gctcgacgcg
      421 gcacgcgaag ccggtctccg gggcgccttc ccggacggaa gctcgctcga cgcggtcgcc
      481 gcgctcgtcg aggcgttcgc cgtgcgcggc gacgtcggcg cgctgaccgc cgcttcccgc
      541 cgtgcctgcg atctcgaggc cgagctcggc ctggcggccc gtccgctcgc ccggctttcg
      601 ctcggctggg cgcagtcgct cgccggcgcc gccgaggcgc gtgcggttct ggaggacgcc
      661 gcctcgtcgg ccgccgagct cggccagccg ctgcacgtct cgatcgcgca cgccctgctc
      721 gcgcgcctcg ccctcgcgga gggcgacggc gatctcgccg aggcgcacgc gcgcgacgcc
      781 gtcgcggcgc tcgcgcgcgg cgagggggac gtcctcgcgt gcggcatggc caacgtcgcg
      841 ctcggcagcg cgctcgcgcg ctcggccgtc gccgaggcgg aggccctgct cgaacggggc
      901 ctcgcggcgc ttcgcgtgct cggcgagccg ctcgcggtcg ccgacgccct gctcgcgctc
      961 gctcccgtgc ggcgctcacg ccagggcctg cccgaggcgc gcgcgtgcat cgccgaggcc
     1021 cgcgagctcg tcgaggccgc cgccgacgcg ggcctcctcg ccggccgcct cgaggaggtg
     1081 gcgcgctcgc tcaccccggc gtaccggaag gcgaacgggg acagcgacct gacggagcgg
     1141 gaggtcgagg tgctccgcta cctcgcggac gggatggcga agcgcgacat cgcgacgacc
     1201 ctcttcctct cgtacaacac gatccactcg cacacgaagt cgatctacca caagctgcgc
     1261 gtgtcctcgc gcgaggacgc cgttcagaag gcgcgcgagc tcggggcgct ctagcgagga
     1321 tcacccgggt gaaccgcccc ggcgggtgaa gccgcccgga cgccgttcca cgatcctcgg
     1381 ggcgtggccg ggaacgacgc agcgaaggcg accggcggag aacgatccca ccgcatcctc
     1441 gtgggcgtcc tcgtcgtcgt cggggcgctg ctcgtcctcg tctcgaccgt cagcgtgtgg
     1501 atccgggatg cggcgctcga caccgacgtc tgggtcgatc agagcgggca gctcctcgag
     1561 agcccggacg tccgcgacgc gctcgccgtc tacatcgtcg accaggcgta ctcggcgggg
     1621 gacgtccagc agcggttgga ggaggggctt ccagaggagt tgcggccgct gtccgggccg
     1681 atcgcggcgc agctccaggg tgtggcctac gacgccgccg cgcgggccct cgcccggccg
     1741 cgcgtccagg agctctggcg aaccgcgaac ggggcggcgc acgagcggat cgtcgccgtg
     1801 ctcgagggcg agtccgagcg ggtcacgctc gagaacggaa acgtcgttct cgagctcgac
     1861 gagctcgtgg cggacgtcgc cgcgcgtgtc ggcgccgggg agggcccgac ggcggccctt
     1921 cagggtcgcg tggagccgat cgtgctcgtg gaatccgagg agctggacgc ggcgcagacg
     1981 agcgtgcggg tcgtgaaggc gctctcgatc tggccgttcc tcctcgggct ggcgctctgg
     2041 acgggcgccg tctacctcgc gcacgggcgc cggcgggaga ccgtccgcgc gatcgcggtg
     2101 agcctcgtcg tgctgggcgt gatcctgctc gtcgtccggc ggatcggcgg caacgtgatc
     2161 gtcgagtcgc tcgtggaggc ggagtccgtg aagccggcgg cggcggtctt ctggtcgacc
     2221 ttcacgacgc tcctgcgcga gtccgcgatc gccggcgtcg tgatcggcgt cctgggcctc
     2281 gtctggacgt ggctgtcggg gcccacgcga ccggccacgg cgatccggga atggctcgcg
     2341 ccgaccttcc gcgaccgcgt cgcgctcgtt cacgggacgc tcgcggcggt catcctgctg
     2401 ctcctcctct gggggcccgt cgggtcgccg cggcgtctcg tctcgctcgt cctcgtcacc
     2461 ggattcgcct tcctcgggct cgagctgctc cgccggcagg ccgtgcggga gtttccgaac
     2521 gccgtgcgcg gtgcagtctc ggcgcgcgcg atctttccgc gcctgccctt cggtcgcgcc
     2581 gaggcgaccg cgggcgagcc ggtggcggcc acgaaggtcg accggctcga gcgcctggcg
     2641 gccctgcacg agcgcggggc gctcacggac gaggagtacc aggccgagaa gcagctcctc
     2701 ctcgcctgag ctccgcggca cggtcgacgc tcactcgagc gacgtgagcg cgagcgcgag
     2761 gccgaagccg agcgtcgtcg cgaggccgac gcgcttgccg ccgtgctcga acgcctcggg
     2821 catcatcgtg tcggcgagca tcgtgaggat cgcgccgccc gcgaacgaga ggacgaacgc
     2881 gagcgtcctc ggcgacgcgt cgccgaaggc cgcgtagccg agcagcgacg cgagcccgcc
     2941 cacgagcgcg acgaccgacc agagaccgag gatccggccg cgcgtccagc cgctcgccgc
     3001 gagcccggtc gtggcggcga tcgcctccgg catgttcgag aggaagacgg ccgcgacgac
     3061 cgcgacgccg acgccc
//
LOCUS       DASTFC010000251         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_368400,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000251 DASTFC010000000
VERSION     DASTFC010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368400"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VFO88_07475"
     CDS             complement(<1..1388)
                     /locus_tag="VFO88_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9545402.1"
                     /translation="MDFQRLTIKSQEAVAAAQELARRAGNPEVYPEHLTIALLGQDLP
                     RTLVERAGHDPEALRAESEAALGSRPRLQGAAQQPTASTAFARVLDDAEREMRRLEDE
                     FVSTEHLLLALDVVDRGALEEALREVRGSQRVTSQDPEGSYQALEKFGRDLTQAAEEG
                     KLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGTGKTAIVEGLAQRIVAGDVPEGLK
                     GKRVWALDIGSLLAGSKYRGEFEERLKAVLGEIQAAAGEIVLFIDELHTIVGAGAAEG
                     AVDAANLLKPMLARGELRAVGATTLDEYRKHVEKDAALERRFQPILVGEPSVSDTIAI
                     LRGLKERYEAHHGVQIRDAALVAAAVLSDRYISDRFLPDKAIDLVDESASRLRMEIDS
                     SPIELDEADRRVRQLEIELAAMAGEKDEVREPLERELAEAKERRDELAARWAEEKEAL
                     TRVAEITKRIDEL"
     gene            complement(1370..1771)
                     /locus_tag="VFO88_07480"
     CDS             complement(1370..1771)
                     /locus_tag="VFO88_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9545403.1"
                     /translation="MSVDERRQPRYMISVAAELVGMHPQTLRIYEAKGLVTPKRTPGN
                     TRLYSEADLERLRLIQQLTTELGLNLAGVEIVLRLQDEVQRLRARMERVERQARDELQ
                     RVHKQYRRDLVLYSDVHDTRPVKASRWTSRG"
     gene            complement(1768..>2558)
                     /locus_tag="VFO88_07485"
     CDS             complement(1768..>2558)
                     /locus_tag="VFO88_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET9545404.1"
                     /translation="GGAGPSARSSRPRGEPGRDVEVEVTVSFEDSLRGATIRIPVELE
                     TACSACNGSGAEPGTVPVVCPECRGRGVVSESQGLFALSQPCPRCGGNGTVVEQPCKQ
                     CKGSGRERRTKRYTVKIPAGVKDGTRIRLKGKGEAGRAGGPPGDLHVVTRVERSPLFE
                     RKGSDLVIDVPVTYAEAALGATVEVPTPEGGRISLKVPPGTQDGRTLRVRGHGAPKLK
                     GSGKGDLLARVRLAVPTRMSKAEREAIEELQKVEKVNPREAAFS"
BASE COUNT          294 a          956 c          915 g          393 t
ORIGIN      
        1 agctcgtcga tccgcttcgt gatctcggcc acgcgcgtga gcgcctcctt ctcctcggcc
       61 cagcgggcgg cgagctcgtc gcggcgctcc ttcgcctcgg ccagctcgcg ctcgagcggc
      121 tcgcgcacct cgtccttctc gccggccatc gccgcgagct cgatctcgag ctggcgcacg
      181 cgccggtcgg cctcgtcgag ctcgatcggc gaggagtcga tctccatccg cagccgcgag
      241 gcggactcgt cgacgaggtc gatcgccttg tccggcagga agcggtcgga gatgtagcgg
      301 tcgctcagca cggccgcggc gacgagcgcg gcgtcgcgga tctggacgcc gtggtgcgcc
      361 tcgtagcgct ccttcagccc gcggaggatg gcgatcgtgt ccgagacgga cggctcgccg
      421 acgaggatcg gctggaagcg ccgctcgagc gccgcgtctt tctcaacgtg ctttctgtac
      481 tcgtcgagcg tcgtcgcgcc gacggcgcgc agctccccgc gcgcgagcat cggcttcagg
      541 aggttcgcgg cgtcgacggc gccctcggcc gcgccggccc cgacgatcgt gtgcagctcg
      601 tcgatgaaga ggacgatctc gccggccgcg gcctggatct cgccgagcac ggccttcagc
      661 cgctcctcga actcgccgcg gtacttcgag cccgcgagga gcgagccgat gtcgagcgcc
      721 cagacgcgct tgcccttgag cccctccggg acgtcgccgg cgacgatccg ctgcgcgagc
      781 ccctcgacga tcgccgtctt cccggtgccg ggctcgccga tcaggacggg gttgttcttc
      841 gtccgccgcg agagcacctg gatcacgcgc cggatctcct cgtcgcggcc gatcaccggg
      901 tcgagcttcc cctcctcggc cgcctgcgtg aggtcgcggc cgaacttctc gagcgcctgg
      961 tacgagccct cggggtcctg cgaggtgacg cgctgcgagc cgcgcacctc gcgcagcgcc
     1021 tcctcgaggg cgccgcggtc gacgacgtcg agcgcgagca ggagatgctc cgtcgagacg
     1081 aactcgtcct cgagccggcg catctcccgc tcagcgtcgt cgaggacgcg ggcgaacgcg
     1141 gtcgaggcgg tcggctgctg cgcggcgccc tggaggcgcg ggcggctgcc gagcgcggcc
     1201 tccgactcgg cgcggagcgc ctccgggtcg tggcccgcgc gctcgacgag cgtgcgcggc
     1261 aggtcctgcc cgagcagcgc gatcgtcagg tgctcggggt acacctccgg attgccggcg
     1321 cggcgcgcga gctcctgggc cgcggcgacc gcttcctggg acttgatcgt cagcctctgg
     1381 aagtccatct ggatgccttc actggtcgcg tgtcgtggac gtcgctgtag aggacgaggt
     1441 cgcgccggta ctgcttgtgg acgcgctgca actcgtcgcg ggcctggcgc tcgacccgct
     1501 ccatccgcgc gcggagccgc tgcacctcgt cctggaggcg caggacgatc tcgacgcccg
     1561 cgaggttcag cccgagctcg gtcgtgagct gctggatgag cctgagccgc tcgaggtcgg
     1621 cctccgagta gagccgcgtg ttgcccggcg tccgcttcgg cgtcacgagg cccttcgcct
     1681 cgtagatccg gagcgtctgc gggtgcattc cgacgagctc ggcggcgacc gagatcatgt
     1741 agcgcggctg tctccgctcg tcgacgctca cgagaacgcg gcctccctcg gattcacctt
     1801 ctccaccttc tggagctcct cgatcgcctc gcgctcggcc ttcgacatgc gcgtcggcac
     1861 agcgaggcgg acgcgggcga gcaggtcgcc cttgccggag cccttgagct tcggcgcgcc
     1921 gtggccgcgg acgcggagcg tgcggccgtc ctgcgtcccc ggcgggacct tgagcgagat
     1981 gcgcccgcct tcgggcgtcg gcacctcgac ggtcgcgccg agcgcggcct ccgcgtacgt
     2041 gaccgggacg tcgatcacga gatcggagcc cttccgctcg aagagcggcg agcgctcgac
     2101 ccgcgtcacg acgtgcaggt cgccgggcgg cccgccggcg cggccggcct cgcccttgcc
     2161 cttcagcctg atgcgcgtcc cgtccttgac gccggccggg atcttcacgg tgtagcgctt
     2221 cgtccgccgc tcgcggccgg agcccttgca ctgcttgcac ggctgctcga cgacggtgcc
     2281 gttgccgccg cagcgcgggc acggctggga gagcgcgaag aggccctggc tctccgagac
     2341 gaccccgcgg ccgcggcact cggggcagac gaccggcacg gtgccgggct ccgcgcccga
     2401 gccgttgcac gccgagcagg cggtctcgag ctcgaccggg atcctgatcg tcgcgccgcg
     2461 cagggagtcc tcgaaggaga ccgtcacctc gacctcgacg tcgcgccccg gctcgccgcg
     2521 cgggcggctc gagcgcgccg acggcccggc gccgccgc
//
LOCUS       DASTFC010000252         6388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_187339,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000252 DASTFC010000000
VERSION     DASTFC010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187339"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..3095
                     /gene="mobF"
                     /locus_tag="VFO88_07490"
     CDS             165..3095
                     /gene="mobF"
                     /locus_tag="VFO88_07490"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HET9545405.1"
                     /translation="MLSVSKLTPGQEAYYERSVAAGLDDYYAGRGESPGVWAGRGAED
                     LGLEGMVEEGELGALVRGRHPKSGAELRRHAAARTIRIERIDPETGERWREEKRLAPV
                     AGFDLVFSTPKSVSLLHALGDETTRLAVDAAHRSAWRAALGYLEEEACVTRRGKNGVR
                     RERGGGFLAAAYQHRTSRAQDPHLHTHVIVANMTRSPSDGEWRALDGEAILKTYRLAA
                     GYLYEAHLRLELSRTLGVEWQEPEKGWAELEGVPRSVIDAFSRRRAQVVERLAEQATS
                     GFYAARVAAVATREPKEEVDLPRLREEWAARAGEHGLGRRELVRLLGRSPYREPTPGE
                     LVRVAERLLGPEGLTEKRSAFSAPELVMAWAEAHTQGASVARIRALSQRFLELPGIER
                     VSEAPPPGRAAYYSTRELLALEREALALVERGRSSGAPAVPAELVEAVLAERARAPAL
                     SSDQERMVHTATASRDRVVAVVGPAGSGKTTAIRAAAEAFAAGGHSVLGGAPSGIAAE
                     RLADEAGIPAATLHRLLAVASAAGGLPGGSVLVVDEAGMAETRVLAPLLRLVEEAEGK
                     AILVGDPGQLPAVGAGGLFVAIVEREGAVELRESRRQPDTLERHALAALRGGLGRDYL
                     AFAENRERLLVSEGPLEARARLLADWWQSAREDLEGSVMIALRRADVAELNAAARALL
                     DAEGKLGKERLEVAGREFGAGDRVVCRRNSEALGVRNGTRGTVAAVHPNERTLTLATD
                     RGERVDLPETYLEAGHVEHAYALTGHACQGLTVERAFVLGSDRGRLQEWGYVALSRAR
                     QQTRLYVTEPVAEAESHFHELDEREPLTRLAQALEQPGAERLASEQRPLPAGPRPGSR
                     PVIARAERPERSRLRLLEKRERETELLRARAERRLAEAEERVAALGWRARGGRGRELR
                     GEIALQHSTLRLANEKRVELERERTQLVERLARAEEAPGRSRRPAPGRGRERGHQLSL
                     DLGL"
     gene            complement(3105..3620)
                     /locus_tag="VFO88_07495"
     CDS             complement(3105..3620)
                     /locus_tag="VFO88_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545406.1"
                     /translation="MAAEERFDPLALVAAFEREGASYVLVGALARVLQGADELTRGVD
                     FTPSLREKNLERVEAALEHLEAQAADRRKTSVAERIVRGEEVIRYRTRAGELKLVPRP
                     EGTEGYDDLRRRARREYLGQGVRAQVASINDLARMLAAREREQDLEPLLALRRLHELE
                     RTIGRSLELGR"
     gene            complement(3610..3927)
                     /locus_tag="VFO88_07500"
     CDS             complement(3610..3927)
                     /locus_tag="VFO88_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9545407.1"
                     /translation="MRSGALLQEARLRAGLTQYELAERTGRDRAVIARWEQEVVAPSL
                     ETLIDLLRACGFDLEPVLVPYETERDERLSANLRLSPERRLERLVRAVEADIQRGGRR
                     RGR"
     gene            4389..5393
                     /locus_tag="VFO88_07505"
     CDS             4389..5393
                     /locus_tag="VFO88_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET9545408.1"
                     /translation="MGKNRRQAERALTKIQARVDEGDYVPQATIRFSAWGERWLASLE
                     RKETTVASYRSTIAYASALFGEKVVRRLLPEDVARFNLALRERGVSASTRAKHLRVLG
                     ACLAAACAHGYAARNPVRQLPKAERPRAERKEAAYFENQELPRLFAEVSPGLFTTVFQ
                     LALKTGIRQGELLALTWGDIDLGEGIVRVRRTITDGHLGLPKNHERRDVDLTADLVAL
                     LGAWWGELGRPTDEKLVFPGDTRSGYLVPTTILRRELYPALARAGIPRIGPTGEARTF
                     HSLRHTFAKRALESGRQITWLSRHLGHSSLKVTTDIYGHWERAERKREAALMEGVFGV
                     "
     gene            complement(5486..5558)
                     /locus_tag="VFO88_07510"
     tRNA            complement(5486..5558)
                     /locus_tag="VFO88_07510"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5523..5525),aa:Lys,seq:ttt)
     gene            5675..>6388
                     /locus_tag="VFO88_07515"
     CDS             5675..>6388
                     /locus_tag="VFO88_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545409.1"
                     /translation="MAEPSWPIDDAGHRRAERRPAARKRRRSHRRARLLVLPAVHVVV
                     LTTFAFAQPLFDLLGRNAEFFTFRGSTRSDLIAFTLGAVLIPPALLLAVEALAMTRGA
                     LVWLAVHLAIVGGLAGLLVLAALHARLGGGAAVIVVSALVGLGASAAYFLVPPVRSVL
                     TVLAPAPLIVSALFLFNSPVSKLLTPEETLSAPAAAVESRAPVVLLVFDEFGTNALLD
                     GRGRIDAKRYPNFARLAGTS"
BASE COUNT          876 a         2392 c         2283 g          837 t
ORIGIN      
        1 gcgcagcgac cggctggtcg ccgtgcaggc tgacgagtac gtcggggttt cggcggcagc
       61 ggtgcggcct gacgaggggc cggacgcacc ggtcggacta ccctagcgat agtggcggga
      121 cagagagacg cagatgcatc cggtgatgcg tttatgcttc ctgagtgctg tcggtttcga
      181 agctgacgcc ggggcaggag gcctactacg agcgttcggt cgcggcgggc ctggacgact
      241 attacgcggg gcggggcgag tcgccgggtg tgtgggcggg ccggggcgcc gaggatctgg
      301 ggctggaggg gatggtcgag gagggcgagc tgggcgcgct cgtgcgcggg cgacatccga
      361 agagcggcgc ggagctccgc cggcacgccg cggcgcgcac gatccggatc gagcggatcg
      421 acccggagac gggcgagcgc tggcgggagg agaagaggct cgccccggtc gccggcttcg
      481 acctcgtctt ctccaccccg aagagcgtca gcctcctcca cgcgctgggc gacgagacaa
      541 ccaggttggc ggtcgacgcg gcgcatcgct ccgcctggag ggccgcgctc ggatatctgg
      601 aggaggaggc gtgcgtgacg cgccggggca agaacggcgt ccggcgggag cggggaggag
      661 gcttcctggc ggccgcctac cagcaccgga cgagccgggc gcaggatccg cacctgcaca
      721 cgcacgtgat cgtcgccaac atgacccgaa gcccctccga cggggagtgg cgggcgctcg
      781 acggcgaggc gatcctcaag acctaccggc tcgccgccgg ctacctgtac gaggcgcacc
      841 tgcgcctcga gctctcccgc accctgggtg tcgagtggca ggagccggag aagggctggg
      901 ccgagctgga gggcgtcccc cggagcgtga tcgacgcctt ttcgcgtcgg cgggcgcagg
      961 tcgtcgagcg cttggcggag caggcgacgt cgggcttcta cgccgcccgg gtggcggcgg
     1021 tcgcgacgcg ggagccgaag gaagaggtcg acctgccgcg cctgcgggag gagtgggccg
     1081 cccgggcggg cgagcacggg ctcggtcgca gggagctcgt gcggctcctc ggccgctccc
     1141 cgtaccgtga gccgactccg ggggagcttg ttcgcgtcgc cgagcggctg ctcgggcccg
     1201 aggggctgac cgagaagcga agcgccttct ctgcgcccga gctcgtgatg gcctgggcgg
     1261 aggcgcacac tcagggcgcc tccgtcgcgc gcatccgggc gctctcgcag cgcttcctcg
     1321 agctgcccgg gatcgagcgg gtgagcgagg cgcccccgcc gggtcgggcc gcctactact
     1381 cgaccaggga gctactcgcg ctcgagcggg aggcgctcgc cctggtcgag cgcggccgga
     1441 gcagcggtgc cccggccgtt cccgccgagc tggtcgaggc ggtgctggcc gagcgggcga
     1501 gggcgcccgc gctctccagc gaccaggagc ggatggtgca caccgcgact gcgagccgcg
     1561 accgcgtcgt cgcggtcgtc gggcccgccg gcagcggaaa gacgacggcg atccgggccg
     1621 ccgccgaggc cttcgcggca ggcggccatt ccgtgctagg cggcgcgccc tccgggatcg
     1681 ccgccgagcg cctcgccgat gaggcgggca ttccggcggc gacgctccac cgcctgctcg
     1741 ccgtcgcgag cgcggcgggc gggctcccgg ggggctccgt cctcgtggtc gacgaggcgg
     1801 ggatggcgga gacgcgcgtg ctcgcgccgc tgcttcgcct cgtcgaggag gcggagggga
     1861 aggcgatcct cgtcggcgac cccgggcagc ttcccgccgt cggagcgggc gggctcttcg
     1921 tcgcgatcgt tgagcgcgag ggggcggtcg agctgaggga gagccgccgc cagccggaca
     1981 cgctcgagcg gcacgcgctc gctgccctgc gcggcgggct cgggcgcgac tacctcgcct
     2041 tcgccgagaa ccgcgagcgg ctcctcgtct cggagggtcc gctcgaggcg cgggcgcggt
     2101 tgctcgccga ctggtggcag tcggcgaggg aggatctgga ggggagcgtg atgatcgcgc
     2161 ttcggcgcgc cgacgtggcc gagctgaacg ctgccgcccg ggcgctcctg gacgccgagg
     2221 gaaagctggg caaggagcga ctcgaggtcg ccgggcggga gttcggcgcc ggcgaccggg
     2281 tcgtctgccg gcgcaactcc gaggcgctcg gcgtccgcaa cggcacccgc gggacggtgg
     2341 ccgcagtcca cccgaacgag cgaacgctca cgctcgccac cgaccggggc gagcgggtcg
     2401 acctccccga gacatacctc gaggcggggc acgtcgagca cgcctacgcg ctcaccggcc
     2461 atgcgtgcca ggggctgacc gtcgagcgcg cattcgtgct cggctccgac cgcgggcgcc
     2521 tgcaggagtg gggctacgtc gccctctccc gcgcccgcca gcagacccgc ctctacgtca
     2581 ccgaacccgt cgcggaggcg gagagccact tccacgagct cgacgagcgc gagcccctca
     2641 cccggctcgc acaggcgctc gagcaaccgg gggccgagcg cctcgcgagc gagcagcgcc
     2701 cgctcccggc agggccgaga ccgggcagcc gtccggtcat cgcccgggcg gagaggccgg
     2761 agcgctcccg tctgcgcctc ctcgagaagc gcgagcggga gacggaactc ctgcgcgcgc
     2821 gcgccgagcg gcgtctggca gaggcggagg agcgcgtcgc ggcgctcggc tggcgcgccc
     2881 gcggcgggcg cggacgcgag cttcgcggcg agatcgccct ccagcactcg acgcttcgcc
     2941 tcgcgaacga gaagcgcgtc gagctcgaac gggagcggac acagctcgtc gagcgcctcg
     3001 cccgcgccga agaggcgcct ggccgcagtc gccggccggc gcccggccgt ggccgggagc
     3061 gcgggcacca gctcagcctg gacctcgggc tgtagacgga acggctaccg gccgagctcg
     3121 agcgagcgcc cgatcgtgcg ctcgagctcg tgcaggcggc gcagggcgag gaggggctcg
     3181 aggtcttgct cgcgctcgcg ggcggcgagc atgcgggcga ggtcgttgat cgaggcaacc
     3241 tgggcacgca cgccctggcc caggtactcg cggcgggcgc ggcggcgcag atcgtcgtag
     3301 ccctccgtcc cctccgggcg cgggacgagc ttcagctctc ccgcccgcgt ccggtagcga
     3361 atcacctcct cgccgcgcac gatccgctcg gcgacggacg tcttgcgccg gtcggcggcc
     3421 tgcgcctcca ggtgctccag ggcggcctcg acccgctcga ggttcttctc ccgcagggag
     3481 ggggtgaagt cgaccccacg ggtcagctcg tccgccccct gcagaacacg ggcgagcgcg
     3541 ccgaccagca catagctcgc cccctcgcgc tcgaaggccg ccacgagcgc gagcgggtcg
     3601 aagcgctcct cagcggccac gccgtctccc accccgctgg atgtccgcct cgaccgcccg
     3661 cacgagccgc tccaaccggc gctcgggcga gagcctcagg ttcgcgctca gccgctcgtc
     3721 gcgctccgtc tcgtagggga cgagcaccgg ctcgaggtcg aagccgcagg cgcgcaacaa
     3781 atcgatcagc gtctccaacg aaggcgcgac cacctcctgc tcccagcgcg cgatcaccgc
     3841 ccggtcacgg ccggtgcgct ccgccagctc gtactgggtc agccccgccc gcagcctcgc
     3901 ctcctgcaag agcgccccac tacgcatcga cgagggagtc taacgcccgg agttgatgcc
     3961 gtcatctgaa gcatatatgc ttcaccgtct ccgatggctc aggcactcgc gcccgcgccc
     4021 agcccctacc tcctcgtcga ggacgtcgcg gcccggctgc gctgctcgcc ccgcacgatc
     4081 cacgagctca cccgcacccg cgccatcccc caccgacggc tccccggcgg acgacgctgc
     4141 ctcttccgcc aagacgaact cgaagcctgg gaacaaggcg cacccctcga gatcctcgag
     4201 acgcccggcg gcggcagagt cgtccggccc cagcactgat ggcaagcctg caagcgagac
     4261 acagccgcac ctgcgccctc ggcaaaccct ggacaccgtt ccagcaggta accgacgggt
     4321 gcacctgccc cggcggaccc acctactacg tcgccgtccg cgaaggatcg aagctccacc
     4381 gcgagcgagt ggggaagaac cgccgccagg ccgagcgggc gctcacgaag attcaggccc
     4441 gagtcgacga gggcgactac gtcccccagg ccacgatccg cttctccgcg tggggcgagc
     4501 gctggctcgc ctctctcgag cgcaaggaga cgaccgtcgc cagctaccgc tcgaccatcg
     4561 cctacgcgag cgccctcttc ggcgagaagg tggtgcgccg cctgctcccc gaggacgtcg
     4621 cccgcttcaa cctcgccctg cgcgagcggg gcgtctccgc ctcgacgcgg gccaagcacc
     4681 tccgcgtcct cggcgcctgc ctcgccgccg catgcgcaca cggctacgcg gcccgcaatc
     4741 ccgtccgcca gctgcccaag gcggagcggc cgcgggccga gcgcaaggaa gccgcctact
     4801 tcgagaacca ggagctcccc cgcctcttcg ccgaggtctc gccggggctg ttcacgacgg
     4861 ttttccagct cgcgctcaag acgggcatac gccagggcga gctcctcgcc ctcacctggg
     4921 gcgacatcga cctcggcgag ggaatcgtcc gcgtgcgccg gacgattacc gacggccacc
     4981 tcggcctgcc caagaaccac gagcgccgcg acgtcgacct caccgccgac ctcgtcgccc
     5041 tcctcggcgc ctggtggggc gagctcggac ggcccacaga cgagaagctc gtcttccccg
     5101 gcgacacgcg gtccggctac ctcgtcccca cgacgatctt gcggcgcgag ctgtaccccg
     5161 ccctggcccg cgccggcatc ccgcgcattg ggcccacggg cgaagcgcgc accttccact
     5221 ccttgcgcca cacgttcgcg aaacgtgcac tcgaatctgg ccgccagatc acctggctct
     5281 ctcgccacct cggccactcg agcctaaagg tcacaaccga catctacggc cactgggagc
     5341 gggccgagcg caaacgcgaa gccgccctca tggaaggcgt cttcggcgtc tgagccgggg
     5401 ccggtcttcc ggatctcgta ctaccacggt acgcgcggcc cccgaacgaa cgactcgaag
     5461 ggcttgcttt gcaggggatt tgaagtgggc cgggctggac tcgaaccagc gaccttggga
     5521 ttaaaagtcc cctgctctac cagctgagct accggcccct cgagcgagtg tagccccggc
     5581 tccccgcgtc ctcagcgcgg tcgccgccct cgaccagcgg gccgggtaag gcgcgggagg
     5641 ttcaccagca cacgcaggtc gtatccttcc gcacgtggcc gaaccgtcgt ggccgatcga
     5701 cgacgccggg caccgccgcg ccgagaggag gcccgcggcc cggaaacgcc gccggtcaca
     5761 ccgtcgagcg cggctcctcg tgctgccggc cgtccatgtc gtcgtcctga cgacgttcgc
     5821 gttcgcccag ccgctcttcg acctcctcgg gcggaacgcc gagttcttca cgttccgcgg
     5881 ctcgacgcgc tccgacctga tcgcgttcac cctgggcgcc gtgctcattc cgccggcgct
     5941 cttgctcgcc gtcgaggcgc tcgcgatgac gcgcggcgcg ctcgtctggc tggcggtcca
     6001 ccttgcgatc gtcggaggcc tcgccgggct cctcgtcctc gccgccctgc acgcccggct
     6061 cggtggcgga gcggccgtca tcgtcgtctc ggccctggtc gggctcggag cgagcgccgc
     6121 ctacttcctc gtcccgcccg tccggtccgt cctcaccgtg ctcgcgccgg cgccgctcat
     6181 cgtctccgcg ctcttcctct tcaactcgcc ggtgtcgaag ctgctgaccc cggaggagac
     6241 gctctcagcc ccggccgccg cggtggagtc gcgcgcgccc gtcgttctcc tcgtcttcga
     6301 cgagttcggc acgaacgctc tcctcgacgg tcgcgggaga atcgacgcga agcgataccc
     6361 gaacttcgcg cgcctcgcgg ggacgtcg
//
LOCUS       DASTFC010000253        12681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1192555,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000253 DASTFC010000000
VERSION     DASTFC010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12681
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192555"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFO88_07520"
     CDS             <1..349
                     /locus_tag="VFO88_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET9545410.1"
                     /translation="AHPGRQLTLMNARVLELVAPSRDGWALAGDQLCVDLDLSVENLP
                     SGTRLAVGTAELEVTDELHTGCAKFTERFGPAAIRFVNGKAGRPLRLRGMYARVVRPG
                     VVRRGDPVRRLPS"
     gene            complement(346..1185)
                     /locus_tag="VFO88_07525"
     CDS             complement(346..1185)
                     /locus_tag="VFO88_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545411.1"
                     /translation="MRRALALLALLPLAACGGAGESRWERVTSPPLTPRVPAATFSSG
                     PEAFVVGGDTEPCPPNAGCVLPRTPPLADGAAYDARTGEWRRIADAPVPFEFADVELL
                     GEAAYLWIVSDGRPGTSTAFLAYRIPDDRWDELPLPPSAEDADEVDVDLVQADGRLVT
                     LTFTDAGDAVPADVLDPAAETWAPAPDGLAATPRSAPRDERLEPHEVAGWIGRYTAPS
                     GWALDEETGEWIEIEPLEEDDDWVDERAVASAGRDLLVVGGVRWEGLEPRLLNDAWLW
                     RAP"
     gene            1312..2154
                     /locus_tag="VFO88_07530"
     CDS             1312..2154
                     /locus_tag="VFO88_07530"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET9545412.1"
                     /translation="MRFRKGARLDPSQVQRRGPGAGGLAVGGGGIGLLVLVVLSLITG
                     TPVDLGGGSGAPSVEEGEPLSECQTGEDANEQRDCRIVGTVNSIQAYWDGQFREGEYR
                     EADTVVFEGSTQTGCGGATSQVGPFYCPRDAQIYLDLGFFQTLESQLGARGGPFAEAY
                     VLAHEYGHHVQNLTGVLGRARSGDTGPGSDAVAVELQADCYAGVWANNAVDTGFIAEL
                     TDDDIARGLDAAAAVGDDRIQERTQGQVNPETWTHGSAEERQRWFTTGYQSGDPGVCD
                     PFSR"
     gene            2667..4586
                     /gene="mbhE"
                     /locus_tag="VFO88_07535"
     CDS             2667..4586
                     /gene="mbhE"
                     /locus_tag="VFO88_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HET9545413.1"
                     /translation="MTAQDAILLFVFWDLTAVASYFLIGYDSHRRDARANALMALLVT
                     GISAVLLLIAAVLLYDEYGTFSLPLLFQAAEPGALVTTAGALVAVAGLAKSAQVPLHF
                     WLPRAMVAPTPVSAYLHSAAMVAAGVFLLGRFYPLIQTSGILLDLLLWTGIASMAVGG
                     ILALTRDVMKQVLAYSTIAQYGYVTFMLGLGGAKGAVGASFYVLAHALMKSALFLTAG
                     AAAEAGGDDRLSRVGGLARTHRALAVGSGLAAAGLAALPLTVGFFKDELFFAAALERG
                     SPWAVVAVGSAALTLAYVARFWVSIFLGPPQGKARAVPVALVWPVVLLGGLVLAGGIV
                     VEPLARLAAAAGEATLGAPTPAEPAYHLDTRAVNLLALATYALGAALFFTRARWAPAA
                     RAVAELGERGGPERLYRGALRGLNLLSDRIHDVEVRDLRTRVASVLVPAGILVLLGIW
                     VTPTGGYDPGSIGTADIGLVLVLVVATFAALATAMTRLHLTLVLVLSALGFSLAVAYA
                     FSGAPDVALVAVLVETIVALLFLGVFALLPREVLRREARLKTSRSRRWRDPLIGLFSG
                     AVAFLVVWSALSRPAPEASVAGDHVRLAPDAHGSDVVTVILADFRGLDTLGEITVLAV
                     ALLGIATLLRRGRTT"
     gene            4583..5017
                     /locus_tag="VFO88_07540"
     CDS             4583..5017
                     /locus_tag="VFO88_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HET9545414.1"
                     /translation="MTTILTETVARLLIAPAFAVAAAILVKGYADVGDGFAAGVVAGL
                     GVLLLFAAFGARATHRVLPVRRAFTVAVSGLLVALVVGFAPLLAGDPPFTHRPAPEEP
                     VVKLGTLELISAVAFDVGVFALVLGVVVGSLDYAARVRKGDW"
     gene            5014..5385
                     /locus_tag="VFO88_07545"
     CDS             5014..5385
                     /locus_tag="VFO88_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET9545415.1"
                     /translation="MILLFSGVVAVLFGTGAFLMLKADLFRVVVGMVMISNAAILALI
                     GSGLSRGAAPIHPLPDDRLSDPLVQAMALTAIVIGFAVTALLLAIAYRVYTSHESVDL
                     DELSREEVRRERELEREEISP"
     gene            5382..6782
                     /locus_tag="VFO88_07550"
     CDS             5382..6782
                     /locus_tag="VFO88_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9545416.1"
                     /translation="MTLLVLPLAVVWLGAAVLALLDGRRRFVGWAAVAVLGANVAGLA
                     ALGARVAEDGRQELVTGNWPRGVGITLAADELGIIFAVLSSAVLLVALLYEVLAGVRS
                     RTFPSLVLFMAAGLTGLFLTGDLFNFFVFFELSMIPAYVLSVYGDQARRKIGAAFIFA
                     VVNLLGSFIFLISVAGTYHVTGTLEMRDAAERLADAEPASVMLIAVGVFVAFGVKLGL
                     FPFHFWLPPIYVGATPAVAAILSGALANIGAYGLLRFGAALLPAELELGAGALIVIGS
                     VSILYGAMQALSRRDVREVLAYSSIGQAGYILVALAVGGRVGFFAAVVYAVVNSANKA
                     LLFLTAGLRGWLVGAAFVVGAFSVAGVPPSAGFFGKVAIFQAGVAEESIALLVLLFVG
                     GALSFVYMFQIYQHAFWRPEASAGGGEPSSAVLRGLTLGLAGVVVAFGIWPEPLLALS
                     GGAADLLAGDSGESAP"
     gene            6779..7273
                     /locus_tag="VFO88_07555"
     CDS             6779..7273
                     /locus_tag="VFO88_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HET9545417.1"
                     /translation="MTKRVLAIALVAGVYLLMLASLDPVDVAISLVVAAVLVLGLGEV
                     LFSGGDLGARDLARRSVRFLPFAAAVVWDIVRGTWAVALVVLHVRALERPGIVAVPVG
                     RRTRTGVAVTGLVATLSPGEFLIDVDWERGLMYLHVLDATDPDGVRERHDAFYRRWQE
                     PVFP"
     gene            7287..7556
                     /locus_tag="VFO88_07560"
     CDS             7287..7556
                     /locus_tag="VFO88_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HET9545418.1"
                     /translation="MHEAVFYVAALWMTGLLVVSVGLVIRARSSLSRILALDMLILIL
                     IALLVLLSDANRVSYYLDAALVLALLSFIATIGAARYHSEGRLFS"
     gene            7553..7942
                     /gene="mnhG"
                     /locus_tag="VFO88_07565"
     CDS             7553..7942
                     /gene="mnhG"
                     /locus_tag="VFO88_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HET9545419.1"
                     /translation="MSAWVADALVLLGVVVMTVGVYGVFRMPDVYTELHAASKAVFLG
                     VVVLLVASTVTGDGAIIARAALIGVFLVLTTPVAAHVIALAAYRQEERMETPGALDES
                     GHDLPHGRAARPDSEPVTVDGDGRRRL"
     gene            8000..8305
                     /locus_tag="VFO88_07570"
     CDS             8000..8305
                     /locus_tag="VFO88_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545420.1"
                     /translation="MATKAATAWGEAAIVDELVLPQEAGEKGFAAVVQLLADGDGGRY
                     VRFAYTTDGVARRGPVTFREADLERLRSELGGHPELAVALGLAPPAARRGGGRKRSR"
     gene            complement(8316..9017)
                     /gene="purQ"
                     /locus_tag="VFO88_07575"
     CDS             complement(8316..9017)
                     /gene="purQ"
                     /locus_tag="VFO88_07575"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET9545421.1"
                     /translation="MSEVRPRIGVVTFPGSNDDRDAVWALAALGADAKPLWHADERLP
                     ALDAIVLPGGFSYGDYLRAGAIARFSPAMGAVVEFAREGGLVLGICNGFQVLCEAGLL
                     PGVLRPNRSLSFVCRDVRLTVERTDTPFTSRCRPGQELTIPVKHGDGCWFAPAALLAE
                     VEGDDLVLLRYAEGENPNGSVADVAGLVSREGNVMGLMPHPEHAVDPLLGSGDGALVL
                     ASLVDAARDRVLQAA"
     gene            complement(9014..9259)
                     /gene="purS"
                     /locus_tag="VFO88_07580"
     CDS             complement(9014..9259)
                     /gene="purS"
                     /locus_tag="VFO88_07580"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET9545422.1"
                     /translation="MRATVLVRPKAGILDPQGSAVEGSLRQLGFPVGEARVGRVIDLD
                     VDAGSTDEARSAVERMCEQLLANPLIESYEIALEDAS"
     gene            complement(9256..10122)
                     /locus_tag="VFO88_07585"
     CDS             complement(9256..10122)
                     /locus_tag="VFO88_07585"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET9545423.1"
                     /translation="MPEAATHVASGKVREIYELDSERLLLVASDRISTFDVVLPTEIP
                     DKGRVLTGLSAFWFARTRDIVPNHVLAVRDDGRSTEVQRLRMLPIECVVRGYITGSGW
                     KDYQATGATSGHTLPAGLRESDRLPEPIFTPATKAQTGHDENIGRDEAAALVGEELFA
                     EVERIALELYRFGAEHAAARGIILADTKFELGLDEGGRLVLGDEALTPDSSRFWPADE
                     YEPGRSQPSFDKQYVRDYCESLGWDKTAPGPELPEEVVAGTRARYVEAFERLTEIPFD
                     DYVADPRVVSAG"
     gene            complement(10115..11440)
                     /gene="purB"
                     /locus_tag="VFO88_07590"
     CDS             complement(10115..11440)
                     /gene="purB"
                     /locus_tag="VFO88_07590"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9545424.1"
                     /translation="MIPRYSRPAMQAVWSDEGKLARWLEVELAALDGWAEVGVVPRED
                     AEAIRTRAVPPTPQRVAEIEERTQHDLAAFVDAVAEGLGPAGRWLHYGLTSSDVLDTA
                     LALQVRDAGALLVAGLERSRDAVVAQAERHRETACVGRTHGVHAEATTFGAKLAGWAF
                     ELERDRARLERALEGMRHGKLAGAVGTYGGGDPEMERVACERLGLVPEPVATQVVPRD
                     RLAELLSVLALCAASLERFATEIRHLARTEVREVQEPFRRGQKGSSAMPHKRNPIVAE
                     RICGLARVVRAAATVGLENVALWHERDISHSSAERVVVPDAFLALDYMLDRFAWLAEG
                     LVVDTERMRRNLDASHGLVFSQRVLLALVESGLERDEAYRLVQRNALRAWDEETDFRA
                     LLEADEEVAARLAPGGIAAAFDLDDALRHVDVLFERLAALTTEKEEAIA"
     gene            complement(11469..11759)
                     /locus_tag="VFO88_07595"
     CDS             complement(11469..11759)
                     /locus_tag="VFO88_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET9545425.1"
                     /translation="MTEPAAPAAPPAPRRTSTLAIAALACGIAGLTVFPLLASFPAVV
                     LGTMARGELRRDPTLGGDGLATAGLVLGWIGVALAVLGLLVFLFLVLLLLGF"
     gene            complement(11756..12256)
                     /gene="purE"
                     /locus_tag="VFO88_07600"
     CDS             complement(11756..12256)
                     /gene="purE"
                     /locus_tag="VFO88_07600"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET9545426.1"
                     /translation="MSRAEEPTPLAVIEDFEPGPLVGILIGSESDRERVQAAVEELNA
                     RGISNELLVLSAHRNPREVAEYASDAALRGVRVIIAAAGMAAALPGAVAANTDLPVIG
                     VPLTSSKTAGGGLDAILSIVQMPPGVPVACVSLDGAKNAAVLAAQILAQGGGYPGSQP
                     GLQSAR"
     gene            complement(12264..>12681)
                     /locus_tag="VFO88_07605"
     CDS             complement(12264..>12681)
                     /locus_tag="VFO88_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HET9545427.1"
                     /translation="RFGDPETQAIVPRIAGGLAEPLARAAAGDLSGVELEVTDEAAVT
                     VVLAAPGYPDAPETGGAVEGLDEAEADGALVFQEGTALRDGRLVGAGGRVLAVTALGD
                     DPAEARGRAYAALERIRLPGAHYRRDIAAGGVRVAG"
BASE COUNT         1454 a         4742 c         4655 g         1830 t
ORIGIN      
        1 ggcgcatccc ggccggcagc tcacgctcat gaacgcccgg gtcctcgagc tcgtcgcccc
       61 ctctcgcgac ggctgggcgc tggccggcga ccagctctgc gtcgacctcg acctgagcgt
      121 cgagaacctc ccgtcgggga cgcggctcgc ggtcggcacg gcggagctcg aggtgacgga
      181 cgagctccac accggctgcg cgaagttcac cgagcgcttc gggcccgcgg cgatccggtt
      241 cgtgaacggg aaagcgggcc ggccgctgcg cctgcgcggg atgtacgcgc gcgtcgtgcg
      301 ccccggcgtc gtccgccgcg gcgacccggt ccggcggctc ccgagctagg gcgccctcca
      361 gagccacgcg tcgttcagga ggcgcggctc gagtccctcc cagcggacgc cgccgacgac
      421 gaggaggtcg cgtccggccg aggcgaccgc gcgctcgtcg acccaatcat cgtcctcctc
      481 gagcggttcg atctcgatcc actcgcccgt ctcctcgtcg agcgcccagc cggacggggc
      541 cgtgtaccgg ccgatccagc ccgcgacctc gtgcggctcc aggcgctcgt cccggggcgc
      601 ggatcggggg gtcgccgcga gcccgtccgg cgccggcgcc cacgtctccg cggccgggtc
      661 gaggacgtcc gcgggaacgg cgtcgccggc gtccgtgaag gtgagcgtga cgaggcggcc
      721 gtccgcctgc acgaggtcca cgtcgacctc gtccgcgtcc tccgcggacg gcgggagcgg
      781 gagctcgtcc cagcggtcgt cggggatccg gtaagcgaga aacgccgtgg acgtccccgg
      841 gcgcccgtcc gacacgatcc agaggtacgc tgcctcgccg agcagctcga cgtccgcgaa
      901 ctcgaacggg acgggcgcgt cggcgatccg gcgccactcg ccggtgcgcg cgtcgtaggc
      961 ggcgccgtcg gcgagcgggg gcgtcctcgg caagacgcag ccggcgttcg gcgggcacgg
     1021 ctcggtgtcg ccgccgacca cgaatgcctc gggcccggac gagaaggtcg ccgcgggaac
     1081 gcgcggcgtg agcggcgggg aggtgacgcg ctcccagcgg gactcgcccg cgcctccgca
     1141 cgcggcaagc gggagcaacg ccaggagggc gagcgcgcgg cgcacgatgt ctcctttgac
     1201 ggtgggcggg cgtggaagtt cacggccgga agagggcggc atcgccgtcg agccgttcgg
     1261 ccgggtgccg gcgtttggcg tccgccgcct tcgtgggaga atcccgtccg gatgcggttc
     1321 cgaaagggag cgaggctcga tccctcgcag gtgcagcggc gcggccccgg ggcgggcggc
     1381 ctcgccgtcg gcggcggcgg catcgggctg ctcgtcctcg tcgtcctctc gctcatcacc
     1441 ggcactcccg tcgacctcgg cggcggaagc ggcgcgccgt ccgtcgaaga gggggagccg
     1501 ctcagcgagt gccagaccgg cgaggacgcg aacgagcagc gcgactgccg gatcgtcggg
     1561 acggtcaact cgatccaggc gtactgggac ggccagttcc gcgagggcga gtaccgggag
     1621 gcggacacgg tcgtcttcga gggctcgacg cagaccggct gcggcggcgc gacgagccag
     1681 gtcgggccgt tctactgccc gcgcgacgcg cagatctacc tcgacctcgg cttcttccag
     1741 acgctcgagt cgcagctcgg cgcgcgcggc gggccctttg cggaggccta cgtgctcgcg
     1801 cacgagtacg gccatcacgt tcagaacctg accggagtgc tcggccgggc gcggagcggc
     1861 gacacggggc cggggagcga cgcggtcgcg gtcgagctcc aggcggactg ctacgcgggc
     1921 gtctgggcca acaacgcggt cgacacgggc ttcatcgccg agctcacgga cgacgacatc
     1981 gcccgcgggc tcgacgccgc ggccgcggtc ggcgacgacc ggatccagga gcggacgcag
     2041 ggccaggtga acccggagac gtggacgcac ggctcggccg aagagcgcca gcggtggttc
     2101 acgaccggct accagagcgg cgatcccggc gtttgcgacc cgttctcgcg ctaggtcgtg
     2161 tcgcgcggac gggcccggcg gcacgcggcg gcgagaggct ccgcacggcc ggcgccggac
     2221 cggcatgatg ggcgaccggg gccgcaacac cgcatgacga gccgggagca cgacatccgc
     2281 gcccacacga gccgcgcccg cgcggccgat cgcctccgcg cgcccgcgcg gcgccgctga
     2341 gcgggctccg ctccgccgcg ggcctcgtcc gggccctctc gctcgtcgtc tccggcggcg
     2401 ggttcgtcgc gatcctcgcc ctctggctga acggcggcgg gagcgtcgac gtcgcctggg
     2461 cgccggcgct cgacctccgc ctcgcgttcg agctcgacgg cctcgccgcc ctctacgcgc
     2521 tcctcgccac cgggatcggc ttcctcgtct tcgcctactc gacccgctac ctgccgctcc
     2581 acctcgagca cgagggccgc ggccccgccg acgagccgcg cttctacgcg ttcctcctgc
     2641 tcttcctcgt ctcgatggtc ggcctcgtga cggcgcagga cgcgatcctc ctcttcgtct
     2701 tctgggacct gaccgcggtc gcgtcgtact tcctcatcgg ctacgacagc caccgccgcg
     2761 acgcgcgcgc gaacgcgctc atggcgctgc tcgtcaccgg gatcagcgcg gtgctcctgc
     2821 tcatcgcggc cgtgctcctc tacgacgagt acgggacgtt ctcgctgccg ctcctctttc
     2881 aggcggccga gccgggcgcg ctcgtcacga cggccggcgc gctcgtcgcg gtcgccgggc
     2941 tcgcgaagag cgcgcaggtg ccgctccact tctggctgcc gcgcgcgatg gtcgcgccga
     3001 cgccggtctc cgcgtacctc cactcggccg cgatggtcgc ggcgggcgtc ttcctgctcg
     3061 ggcgcttcta cccgctcatc cagacgagcg gaatcctgct cgacctcctg ctctggacgg
     3121 ggatcgcgtc gatggcggtc ggcgggatcc tcgcgctcac gcgcgacgtg atgaagcagg
     3181 tgctcgcgta ctcgacgatc gcgcagtacg gctacgtgac gttcatgctc ggcctcggcg
     3241 gggcgaaggg cgcggtcggc gcgtcgttct acgtgctcgc gcacgcgctc atgaagagcg
     3301 cgctcttcct cacggccggc gcggccgcgg aggcgggcgg cgacgaccgg ctctcgcgcg
     3361 tcggcgggct cgcgcggacg caccgcgcgc tcgcggtcgg gagcggtctc gccgccgccg
     3421 gcctcgccgc gctcccgctc accgtcgggt tcttcaagga cgagctcttc ttcgccgccg
     3481 cgctcgagcg cgggtcgccc tgggcggtgg tcgcggtcgg ctcggcggcg ctcacgctcg
     3541 cgtacgtcgc acggttctgg gtctcgatct tcctcggccc gccgcagggg aaggcccgcg
     3601 ccgtcccggt ggcgctcgtc tggccggtcg tcctgctcgg tggactcgtc ctcgcgggcg
     3661 ggatcgtcgt cgagccgctc gcgcgcctgg ccgccgccgc cggggaggcg acgctcggcg
     3721 ccccgacgcc cgccgagccc gcgtaccacc tcgacacgcg cgcggtgaac ctgctcgcgc
     3781 tcgcgacgta cgcgctcggc gcggcgctct tcttcacgcg cgcgaggtgg gcgccggccg
     3841 cgcgcgcggt cgccgagctg ggcgagcggg gcgggcccga gcggctctac cgcggggcgc
     3901 tccgcggcct gaacctcctc tccgaccgga tccacgacgt cgaggtgcgc gacctgcgaa
     3961 cgcgcgtcgc ctcggtcctc gtgcccgccg ggatcctcgt cctgctcggg atctgggtca
     4021 cgccgaccgg cggctacgac ccgggctcga tcggcaccgc ggacatcggg ctcgtcctcg
     4081 tcctcgtcgt ggccacgttc gccgcgctcg cgacggcgat gacgcggctg cacctgacgc
     4141 tcgtgctcgt cctctcggcg ctcggcttca gcctcgccgt cgcgtacgcc ttctcgggcg
     4201 cgcccgacgt ggcgctcgtc gccgtgctcg tcgagacgat cgtcgcgctc ctcttcctcg
     4261 gcgtcttcgc gctcctgccg cgcgaggtgc tccgccggga ggcgcgcctg aagacgagca
     4321 ggagccgacg ctggcgtgac ccgctcatcg ggctcttctc cggcgccgtc gcgttcctcg
     4381 tcgtctggag cgccctctcg cgccccgcgc ccgaggcaag cgtcgcgggc gaccacgtcc
     4441 ggctcgcgcc tgacgcgcac ggaagcgacg tcgtgaccgt gatcctcgcc gacttccgcg
     4501 gcctcgacac gctcggggag atcaccgtcc tcgcggtcgc gctcctcggg atcgcgacgc
     4561 tcctgcggcg ggggaggacg acgtgacgac gattctcacg gagacggtcg cgcggctgct
     4621 catcgcgccg gcgttcgccg tcgcggcggc gatcctcgtc aaggggtacg ccgacgtggg
     4681 agacgggttc gcggccggcg tcgtggccgg gctcggcgtc ctgctcctct tcgccgcctt
     4741 cggcgcgcgc gccacgcacc gcgtcctgcc cgtgcggcgc gcgttcacgg tggccgtctc
     4801 cgggctgctc gtcgcgctcg tcgtcggctt cgcgccgctc ctcgccggcg accccccgtt
     4861 cacccaccgg ccggcgcccg aggagccggt cgtgaagctc gggacgctcg agctgatcag
     4921 tgcggtcgcc ttcgacgtgg gcgtcttcgc gctcgtgctc ggcgtcgtcg tcggctcgct
     4981 cgactacgcg gcccgcgtgc ggaaggggga ctggtgatcc tcctcttctc cggggtcgtc
     5041 gccgtgctct tcggcaccgg cgcgttcctc atgctgaagg ccgacctctt ccgggtcgtc
     5101 gtcgggatgg tgatgatctc gaacgcggcg atcctcgcgc tgatcggctc ggggctctcg
     5161 cgcggcgcgg ccccgatcca cccgctgccg gacgaccggc tgagcgaccc gctcgtgcag
     5221 gcgatggcgc tgaccgcgat cgtgatcggc ttcgccgtga ccgcgctcct ccttgcgatc
     5281 gcctaccggg tctacacgtc gcacgagagc gtcgacctcg acgagctctc gcgcgaggag
     5341 gtccggcgcg agcgcgagct cgagcgggag gagatctcgc cgtgaccctc ctcgtcctcc
     5401 cgctcgcggt cgtctggctc ggagcggcgg tactcgcgct tctcgacggg cggcgccgct
     5461 tcgtcggctg ggccgcggtg gccgtgctcg gcgcgaacgt cgccggcctc gccgcgctcg
     5521 gcgcgcgggt ggcggaggac ggccggcagg agctcgtcac cggcaactgg ccgcgcgggg
     5581 tcgggatcac gctcgccgcc gacgagctcg gcatcatctt cgccgtcctc tcgagcgcgg
     5641 tgctcctcgt cgcgcttctc tacgaggtgc tcgccggcgt gcgctcgcgc acgttcccct
     5701 cgctcgtcct cttcatggcc gccgggctca cggggctctt cctgaccggc gacctcttca
     5761 acttcttcgt cttcttcgag ctctcgatga tcccggcgta cgtgctctcg gtctacgggg
     5821 accaggcgcg ccggaagatc ggggccgcgt tcatcttcgc ggtcgtgaac ctgctcgggt
     5881 cgttcatctt cctcatctcc gtcgcgggca cgtaccacgt gaccgggacg ctcgagatgc
     5941 gcgacgcggc ggagcggctc gcggacgcgg agccggcctc cgtgatgctg atcgcggtcg
     6001 gcgtcttcgt cgcgttcggg gtgaagctcg ggctcttccc gttccacttc tggctgccgc
     6061 cgatctacgt cggcgccacg cctgcggtcg cggcgatcct gagcggcgcg ctcgcgaaca
     6121 tcggcgccta cgggctgctg cggttcgggg cggcgctcct gccggccgag ctcgagctcg
     6181 gcgcgggggc gctcatcgtg atcggctcgg tctcgatcct ctacggggcg atgcaggcgc
     6241 tctcccggcg ggacgtgcgc gaggtgctcg cgtactcctc gatcgggcag gccggctaca
     6301 tcctcgtcgc gctcgccgtc ggagggcgcg tcgggttctt cgcggccgtc gtctacgcgg
     6361 tcgtgaactc ggcgaacaag gcgctcctct tcctcacggc cggcctgcgg ggctggctcg
     6421 tcggcgcggc gttcgtcgtc ggggcgttct ccgtcgcggg cgtgccgccg tccgcggggt
     6481 tcttcgggaa ggtggcgatc ttccaggccg gggtggcgga ggagtcgatc gcgctcctcg
     6541 tgctgctctt cgtcggcggc gcgctctcgt tcgtctacat gttccagatc taccagcacg
     6601 cgttctggcg gcccgaggcg tcggcgggcg gcggggaacc gagctcggcg gtgctgcgcg
     6661 ggctcacgct cgggctggcg ggagtcgtcg tcgcgttcgg gatctggccg gagccgctgc
     6721 tcgcgctctc gggcggcgcg gccgacctgc tcgccggaga ctccggagag agcgcgccgt
     6781 gacgaagcgg gtgctcgcga tcgcgctcgt ggccggcgtc tacctcctga tgctggcgag
     6841 cctcgatccc gtggacgtgg cgatcagcct cgtggtggca gcggtgctcg tgctcgggct
     6901 cggcgaggtg ctcttttccg gcggcgatct cggcgcgcgc gacctcgcgc gccggtcggt
     6961 ccgcttcctg ccgttcgccg ccgccgtcgt ctgggacatc gttcgcggca cctgggcggt
     7021 cgcgctcgtc gtcctgcacg tgcgcgcgct cgagcggccc gggatcgtgg ccgtcccggt
     7081 cgggaggcgg acgcgcaccg gggtcgcggt cacggggctc gtggcgacgc tctcgccggg
     7141 cgagttcctc atcgacgtcg actgggagcg cgggctcatg tacctccacg tgctcgacgc
     7201 gaccgatccg gacggcgtgc gcgagcgcca cgacgcgttc taccgccgct ggcaggagcc
     7261 ggtcttcccg tgaggcgggc gcgctgatgc acgaggcggt cttctacgtc gccgcgctct
     7321 ggatgaccgg gctcctggtc gtgagcgtcg ggctcgtgat ccgggcgcgc tcgtcgctct
     7381 cgcggatcct cgcgctcgac atgctgatcc tcatcctgat cgcgctgctc gtgctgctct
     7441 cggacgcgaa ccgggtctcg tactacctcg acgcggcgct cgtcctcgcg ctgctctcct
     7501 tcatcgcgac gatcggcgcg gcgcgctacc acagcgaggg gaggctcttc tcgtgagcgc
     7561 ctgggtcgcg gacgcgctcg tcctgctcgg cgtcgtcgtg atgacggtcg gggtctacgg
     7621 cgtcttccgg atgcctgacg tctacaccga gctccacgcg gcgagcaagg cggtgttcct
     7681 cggggtcgtg gtgctgctcg tcgcgtcgac cgtgaccggc gacggcgcga tcatcgcgcg
     7741 cgcggcgctg atcggcgtct tcctcgtcct cacgacgccg gtcgcggcgc acgtgatcgc
     7801 gctcgcggcg taccggcagg aggagcggat ggagacgccc ggcgccctcg acgagtcggg
     7861 acacgacctg ccccacgggc gggccgcgcg gccggactcg gagccggtga ccgtggacgg
     7921 cgacggccgc cggcggttgt gacggtttcg gcacgaagtc gtcacattcc acgcgctttg
     7981 cccgtgtacg ttggcctcga tggcgacgaa ggcggcaacg gcgtggggcg aggcggcgat
     8041 cgtcgacgag ctcgtgctcc ctcaggaggc gggggagaag ggcttcgccg cggtcgtcca
     8101 gctcctggcg gacggcgacg gcgggcgcta cgtgcgcttc gcgtacacga cggacggcgt
     8161 cgcccggcgc gggccggtca cgttccggga ggcggacctg gagcggctcc ggtcggagct
     8221 cggcgggcac ccggagctcg cggtcgcgct cgggctcgcg ccgccggcgg cccggcgggg
     8281 aggtggtcgc aagcgatcgc gctgacgaca gacgatcagg cggcctggag gacgcggtcg
     8341 cgcgcggcgt cgacgagcga cgcgaggacg agcgcgccgt cgcccgagcc gaggagcgga
     8401 tcgacggcgt gctcgggatg cggcatgagg cccatgacgt tcccctcgcg gctcaccagg
     8461 cccgcgacgt ccgcgacgga gccgttcggg ttctcgccct ccgcatagcg gagcaggacg
     8521 aggtcgtcgc cctcgacctc ggcgaggagc gcggcgggcg cgaaccagca gccgtcgccg
     8581 tgcttcacgg ggatcgtgag ctcctgtccg ggccggcagc gggacgtgaa cggcgtgtcg
     8641 gtccgctcga cggtcaggcg aacgtcgcgg cagacgaacg agagcgagcg gttcgggcgc
     8701 aggacgccgg ggaggagccc ggcctcgcag agcacctgga acccgttgca gatcccgagc
     8761 acgaggccgc cctcgcgagc gaactcgacg accgcgccca tcgccggcga gaaccgggcg
     8821 atcgcccctg cccgcaggta gtcgccgtac gagaacccgc cggggaggac gatcgcgtcg
     8881 agcgcgggga ggcgttcgtc cgcgtgccag agcggctttg cgtccgcccc gagcgcggcg
     8941 agcgcccaga cggcgtcccg gtcgtcattc gaccccggga acgtgacgac gccgatgcgg
     9001 ggacgcacct cgctcacgag gcgtcctcca gcgcgatctc gtagctctcg atgagcggat
     9061 tcgcgaggag ctgctcgcac atccgctcga cggcgctccg cgcctcgtcc gtcgacccgg
     9121 cgtcgacgtc gaggtcgatc acgcggccga cgcgcgcctc gcccaccggg aagccgagct
     9181 ggcggagcga cccctcgacg gccgatccct gcgggtcgag gatcccggcc ttcgggcgga
     9241 caaggacggt cgccctcatc cggcgctcac cacgcgtggg tcggcgacgt agtcgtcgaa
     9301 cgggatctcg gtcaggcgct cgaacgcctc gacgtagcgc gcccgggtgc ccgcgacgac
     9361 ctcctcgggc agctccggtc ccggcgccgt cttgtcccag ccgagtgact cgcagtagtc
     9421 ccgcacgtac tgcttgtcga acgacggctg ggagcgcccg ggctcgtact cgtcggcggg
     9481 ccagaagcga gacgagtccg gcgtcagcgc ctcgtcgccg aggacgagcc gcccgccctc
     9541 gtcgaggccg agctcgaact tcgtgtccgc gaggatgatc ccgcgcgccg ccgcgtgctc
     9601 ggccccgaag cggtagagct cgagcgcgat ccgctcgacc tccgcgaaga gctcctcgcc
     9661 gacgagcgcc gcggcctcgt cccggccgat gttctcgtcg tgccccgtct gcgccttcgt
     9721 cgcgggggtg aagatcggct cgggcagccg gtccgactcc cgcagcccgg ccggcagcgt
     9781 gtgccccgag gtcgcgccgg tcgcctggta gtccttccag cccgagcccg tgatgtagcc
     9841 ccggacgacg cactcgatcg gcagcatccg gaggcgctgc acctccgtgg agcggccgtc
     9901 gtcgcggacg gcgagcacgt ggttcgggac gatgtcgcgc gtgcgcgcga accagaacgc
     9961 ggagagcccg gtgagcacgc ggcccttgtc ggggatctcc gtcgggagga cgacgtcgaa
    10021 cgtcgagatc cggtcgctcg ccacgagcag cagccgctcg gagtcgagct cgtagatctc
    10081 ccgcaccttc ccgctcgcga cgtgggtggc ggcctcaggc aatcgcttcc tccttctcgg
    10141 tcgtgagcgc cgcgaggcgc tcgaacagga cgtccacatg gcggagcgcg tcgtcgaggt
    10201 cgaacgcggc cgcgatcccg cccggggcga gccgcgcggc gacctcctcg tccgcctcga
    10261 ggagcgcccg gaaatccgtt tcctcgtccc aggcccggag cgcgttgcgc tgcacgagcc
    10321 ggtacgcctc gtcgcgctcg agccccgact cgacgagcgc cagcaggacg cgctggctga
    10381 agacgagccc gtgcgaggcg tcgaggttcc gccgcatccg ctccgtgtcg acgacgaggc
    10441 cctcggcgag ccaggcgaag cggtcgagca tgtagtcgag cgcgaggaac gcgtccggga
    10501 cgacgacgcg ctcggcactc gagtgtgaga tgtcccgctc gtgccagagc gcgacgttct
    10561 cgagcccgac ggtcgcggcg gcccgcacga cgcgcgcgag cccgcagatc cgctccgcga
    10621 cgatcgggtt gcgcttgtgc ggcatggcgg acgagccctt ctggccgcgg cggaacggct
    10681 cctgcacctc gcgcacctcc gtccgcgcga ggtggcggat ctcggtcgcg aaccgctcga
    10741 gcgacgccgc gcagagcgcg agcaccgaaa gcagctccgc gagccggtcg cgcggcacga
    10801 cctgcgtcgc cacgggctcc ggcacgaggc cgagccgctc gcaggcgacg cgctccatct
    10861 ccggatcgcc gcccccgtac gtcccgaccg cgccggcgag cttgccgtgg cgcatcccct
    10921 cgagcgcgcg ctcgagccgg gcccggtcgc gctcgagctc gaacgcccag ccggcgagct
    10981 tcgcgccgaa cgtcgtcgcc tccgcgtgga cgccgtgcgt gcggccgacg cacgccgttt
    11041 cgcgatggcg ctccgcctgg gccacgaccg cgtcgcgcga ccgctcgagg ccggcgacga
    11101 gaagcgcgcc cgcgtcgcgc acctggagcg cgagcgcggt gtcgagcacg tccgaggacg
    11161 tgaggccgta gtggagccac cgcccggccg gcccgagccc ctccgcgacg gcgtcgacga
    11221 acgccgcgag gtcgtgctgc gtccgctcct cgatctcggc gacccgctgg ggcgtcggcg
    11281 gcacggcacg cgtgcggatc gcctcggcgt cctcccgcgg cacgacgccg acctcggccc
    11341 atccgtccag cgcagccagc tcgacctcga gccagcgcgc gagcttgccc tcgtccgacc
    11401 agacggcctg catggccgga cgcgagtagc ggggaatcac cgtcccgatt ctagaggtgg
    11461 tctcggactc agaacccgag aaggagcaag acgaggaaga ggaagacgag gagcccgagc
    11521 accgcgagcg cgacgccgat ccacccgagc acgagcccgg ccgtcgccaa cccgtcgccc
    11581 ccgagcgtcg ggtcgcggcg gagctcgccg cgcgccatcg tcccgagcac gaccgccggg
    11641 aaggacgcga gcagcgggaa cacggtcagc ccggcgatcc cgcacgcgag cgccgcgatc
    11701 gcgagcgtgc tcgtgcgccg cggggccggc ggcgcggccg gcgcggccgg ctccgtcatc
    11761 gcgcgctctg caggccgggc tgcgagccgg ggtagccgcc gccctgggcg aggatctgcg
    11821 ccgcgagcac cgcggcgttc ttcgccccgt cgagcgagac gcaggcgacc ggcacgccgg
    11881 gcggcatctg gacgatcgag aggatcgcgt cgagcccgcc gcccgccgtc ttcgagctcg
    11941 tcagcgggac cccgatcacc ggcaggtcgg tgttcgcggc gaccgccccg ggcagcgccg
    12001 ccgccatccc ggccgcggcg atgatcacgc gcacgccgcg cagcgccgcg tccgaggcgt
    12061 actcggccac ctcgcgcggg ttgcggtgcg ccgaaaggac gaggagctcg ttcgagatcc
    12121 cgcgtgcgtt cagctcctcg acggcggcct ggacgcgctc gcggtcggac tcggacccga
    12181 tcaggatccc cacgagcggg ccgggctcga agtcctcgat caccgcgagg ggggtcggct
    12241 cctccgcgcg gctcacgccg ggatcagcct gcgacacgaa cgcctcccgc cgcgatgtcc
    12301 cgccggtagt gcgcgccggg cagcctgatc cgctcgagcg ccgcgtacgc ccgcccgcgc
    12361 gcctcggctg ggtcgtcccc gagcgccgtc acggcgagca cgcggccgcc ggcgccgacg
    12421 agccggccgt cccggagcgc cgtcccctcc tggaagacga gcgcgccgtc ggcctcggcc
    12481 tcatccagtc cctcgaccgc gccgcccgtc tccggtgcgt cgggataccc cggcgccgcg
    12541 aggacgaccg tgaccgccgc ctcgtccgtg acctccagct cgacgccgct caggtcgccg
    12601 gccgccgcgc gcgcgagcgg ctccgcgagg ccgccggcga tgcgcggcac gatcgcctgc
    12661 gtctccgggt cgccgaagcg g
//
LOCUS       DASTFC010000254         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_813704,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000254 DASTFC010000000
VERSION     DASTFC010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813704"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFO88_07610"
     CDS             complement(<1..743)
                     /locus_tag="VFO88_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9545428.1"
                     /translation="MSLDRIRNFSIVAHIDHGKSTLADRILEVTSTLTAREMREQVLD
                     SMELERERGITIKAQAVRVDWKGHQLNLIDTPGHVDFTYEVSRALQACEGALLVVDAA
                     QGIQAQTLANAYLALENDLEIVPVVNKIDLPSADPEAAAAEVADLIGEDPERVVRISA
                     KTGAGVEDVLDAIVERIPPPAGDPAAPGRALVFDSAYDQYRGVVAFVRVVDGEFRPRE
                     RLRAMAQGTTFETEELGFFSPERTPTPTLS"
     gene            complement(740..2350)
                     /gene="murJ"
                     /locus_tag="VFO88_07615"
     CDS             complement(740..2350)
                     /gene="murJ"
                     /locus_tag="VFO88_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET9545429.1"
                     /translation="MPPRDDAPGELDADPARPRTRRLARSTAIFSIATGLSRILGLVR
                     EIVVRRYFGVEGSINTFTVAFQIPNLVRSLVADAALGAAFVPVFSELLEKRDSLRAWR
                     VASSLFWLTLLALGGLTALFILTAPWLMRPFGYEGAEHELVVNLSRILFPTVVLLGLS
                     GIVVAILNSYNQFTVPALAPVAWNLVIILGLVLGVPRTEGDDALYVYAGSVLVATVVQ
                     LLLPIPWLRGRDDRLRAVIDWRDPALRRVLVLMLPVTLGLGLINFNQVVNTFFAARFI
                     DRDLAPAAIEAAFRLYMLPQGIFSVAIATILFPSLSRLAARGDLDAFRSTVALGLRQI
                     GFLLIPASAVSAALAVPIVRLLYERGAFGPEQTEVVAASLAAFAAGLTFNGTMLMLNR
                     AFFSLQSPWIPTWIALANLGFQVVLNVALYRVGVWGIPLATSLVNLAGTIGLVLVIRR
                     RVGRIEGRRTLDTYARSLAAAALAAGIAFAVWYGLDEVLGRSGLAQVVSVGSALAASA
                     AVYLAAARILGIRELDALLSLVRRSRTP"
     gene            2489..2758
                     /gene="rpsT"
                     /locus_tag="VFO88_07620"
     CDS             2489..2758
                     /gene="rpsT"
                     /locus_tag="VFO88_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET9545430.1"
                     /translation="MPNIKQQERRVRSAARERMQNLRWRSTAKTLYRRLEVAVADGDE
                     QQVEAAHGELVRWLDKAAARRAVHPNKVARKKSQAARLVAQAKSS"
     gene            complement(2773..3750)
                     /gene="holA"
                     /locus_tag="VFO88_07625"
     CDS             complement(2773..3750)
                     /gene="holA"
                     /locus_tag="VFO88_07625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET9545431.1"
                     /translation="MAADELRPAYLLLGGDRPKIRRALARLRSRFPAESVELLSADTT
                     PAADAVAACNALGLFGDEGARLVVVEGVERWRADDAEAIAAYLESPAASSVLALVAEG
                     AIRQAALVQTVEKAGQVLRFDVPKPRDPSGWVRAEFERVGARADAEAARALVELVGDD
                     VTELASEVDKLATWAAGEEIGRREVEALAVSAHDTFVWALTDAWGDRDCARALAACES
                     LLERRTKEPFAIAAALAGHVGRVRAAQRLAQEGANAAEVARRLKIKDYPARKALGHAR
                     NFGPDELDDAVVRLAELDAALKGASRLAAELEVERALVDVTARREPAAA"
     gene            complement(3764..>4405)
                     /locus_tag="VFO88_07630"
     CDS             complement(3764..>4405)
                     /locus_tag="VFO88_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9545432.1"
                     /translation="VADELSALGIRRLDALVLTHPQRDHVGGAAEVLERVDVGFVLDP
                     GLAGTSEERDEALAAAAEQGVPVVTAGAGRRYRLGRLELEVLWPDGPGPPGGDPNDRA
                     IVLLATYGTTDVLLTADAESHVLLPLRLPEIEVLKVPHHGSADERLPALLDETRPRVA
                     VISVGRSNSYGHPAPSTIAALDAEAGVAVYRTDRDGDVVVESDGSRLTVRVGG"
BASE COUNT          645 a         1535 c         1694 g          531 t
ORIGIN      
        1 gagagcgtcg gcgtcggcgt gcgctccggg gagaagaacc cgagctcctc cgtctcgaac
       61 gtcgtcccct gcgccatcgc gcgcagccgc tcccgggggc ggaactcgcc gtcgacgacg
      121 cggacgaagg cgacgacgcc gcggtactgg tcgtacgcgg agtcgaagac gagcgcgcgg
      181 ccgggcgcgg ccgggtcgcc cgcgggcggc gggatccgct ccacgatcgc gtccagcacg
      241 tcctcgacgc cggcgccggt cttcgccgag atgcggacga cccgctcggg gtcctcgccg
      301 atcaggtcgg ccacctcggc cgcggcggcc tcgggatcgg cggacgggag gtcgatcttg
      361 ttcacgaccg ggacgatctc gaggtcgttc tcgagcgcga ggtacgcgtt cgcgagcgtc
      421 tgcgcctgga ttccctgcgc cgcgtcgacg acgaggagcg cgccctcgca cgcctggagc
      481 gcgcgcgaga cctcgtacgt gaagtccacg tggcccggcg tgtcgatcag gttgagctgg
      541 tggcccttcc agtcgacgcg taccgcctgc gccttgatcg tgatcccgcg ctcgcgctcg
      601 agctccatcg agtcgagcac ctgctcgcgc atctcgcgcg ccgtgagcgt gctcgtcacc
      661 tcgaggatgc ggtcggcgag cgtcgacttg ccgtggtcga tgtgcgcgac gatcgagaag
      721 ttgcggatgc ggtcgaggct catggggtgc gggagcgccg gacgagcgag agaagtgcgt
      781 cgagctccct gatcccaagg atacgggcgg ccgcgagata gacggccgcc gacgcggcga
      841 gcgcggatcc gacggagacg acctgcgcga gcccggagcg gccaagcacc tcgtcgagcc
      901 cgtaccagac cgcgaacgcg atcccggcgg cgagcgcggc ggccgcgagc gagcgcgcgt
      961 acgtgtcgag cgtgcgcctc ccctcgatgc gcccgacccg acggcggatg acgagaacga
     1021 ggccgatcgt ccccgcgagg ttcacgagcg acgtcgcgag cgggatcccc cacacgccga
     1081 ccctgtagag cgcgacgttc aggacgacct ggaagccgag gttcgcgagc gcgatccagg
     1141 tcgggatcca gggcgactgg aggctgaaga acgcgcggtt cagcatgagc atcgtcccgt
     1201 tgaacgtgag gccggcggcg aacgcggcca gcgaggcggc gacgacctcg gtctgctccg
     1261 gcccgaaggc gccgcgctcg tagaggaggc ggacgatcgg cacggcgagc gcggcggaga
     1321 cggcgctcgc ggggatcagg agaaacccga tctggcgcag cccgagcgcg acggtcgagc
     1381 ggaaggcgtc caggtctccg cgtgcggcga gccgcgagag cgaggggaag aggatcgtcg
     1441 cgatcgcgac ggagaagatc ccctgcggga gcatgtaaag gcgaaaggcc gcctcgatgg
     1501 cggccggcgc gaggtcccgg tcgatgaacc gggccgcgaa gaacgtattc acgacctggt
     1561 tgaagttgat gagcccgagc ccgagcgtga ccgggagcat cagcacgagg acgcggcgga
     1621 gcgcggggtc gcgccagtcg atcaccgcgc gcagccggtc gtcgcggccc cgcagccacg
     1681 ggatcgggag gagcagctgg acgaccgtcg cgacgagcac gctgcccgcg tagacgtaga
     1741 gcgcgtcgtc tccctccgtc cgcgggacgc cgaggacgag cccgaggatg atcacgaggt
     1801 tccacgcgac cggtgcgagc gccgggacgg tgaactggtt gtagctgttc aggatcgcga
     1861 cgacgattcc ggagagcccg agcaggacga cggtcgggaa gaggatccgg gagaggttca
     1921 cgacgagctc gtgctcggcg ccctcgtagc cgaacggacg catgagccac ggcgcggtga
     1981 ggatgaagag cgccgtcagc cctccgaggg cgaggagcgt gagccagaag agcgatgacg
     2041 cgacccgcca ggcgcggagg ctgtcgcgtt tctccagaag ctcgctgaac accgggacga
     2101 acgccgcccc gagcgcggcg tcggcgacga gcgaccggac gaggttcggg atctggaacg
     2161 cgaccgtgaa cgtgttgatc gagccctcga cgccgaagta gcggcggacg acgatctcgc
     2221 ggacgagccc gaggatccgc gagagcccgg tcgcgatcga gaagatcgcg gtcgaccgcg
     2281 cgaggcgacg cgtccggggg cgcgcagggt cggcgtcgag ctcgccgggc gcgtcgtcgc
     2341 ggggcggcat gagcgggatc gtagggaagc gaccctctgg agccggtcgg ctctcgtgcg
     2401 gacggggcgt cgtgccgcgc gccgactatg cgcccgcgct ggtacgatcc gtcggcgcgc
     2461 ggcaccggcc gcgctctttt tccgtctgat gccgaacatc aagcaacagg aacgccgtgt
     2521 gcgaagcgcg gcccgcgagc ggatgcagaa cctccgctgg cggtcgaccg cgaagacgct
     2581 ctaccggcgg ctcgaggtcg ccgtcgccga cggcgacgag cagcaggtcg aggccgccca
     2641 cggcgagctc gtccgctggc tcgacaaggc cgcggcgcgg cgtgcggtcc acccgaacaa
     2701 ggtggcgcgc aagaagtcac aggccgcgcg gctcgtcgcg caggcgaaga gctcctaggc
     2761 gcccgctccg gatcaggcgg ccgcgggctc gcgccgcgcg gtcacgtcga cgagcgcccg
     2821 ctcgacctcg agctcggccg cgagccggct cgcgcccttg agcgcggcgt cgagctccgc
     2881 gaggcggacg acggcgtcgt ccagctcgtc ggggccgaag ttgcgcgcgt ggccgagcgc
     2941 cttcctcgcc gggtagtcct tgatcttcag tcgcctcgcc acctcggccg cgttcgcgcc
     3001 ctcctgcgcc aggcgctggg ccgcgcggac ccggccgacg tggcctgcca gcgcggcggc
     3061 gatcgcgaac ggctccttcg tccggcgctc gaggaggctc tcgcatgcgg cgagcgcccg
     3121 cgcgcagtcg cggtcgcccc aggcgtcggt gagcgcccag acgaacgtgt cgtgcgcgct
     3181 cacggcgagc gcctcgacct cgcgccggcc gatctcctcg cccgcggccc acgtggcgag
     3241 cttgtcgacc tcgcttgcga gctcggtcac gtcgtcgccg acgagctcga cgagggcgcg
     3301 cgcggcctcc gcgtcggcgc gcgcgccgac gcgctcgaac tcggcgcgca cccagcccga
     3361 cgggtcgcgc ggcttcggca cgtcgaagcg gagaacctgg ccggccttct cgaccgtctg
     3421 aacgagcgcc gcctgccgga tcgccccctc ggcgacgagc gcgaggacgc tcgacgcggc
     3481 ggggctctcg aggtacgcgg cgatcgcctc cgcgtcgtcc gcgcgccagc gctcgacgcc
     3541 ttcgacgacc acgagccgtg cgccctcgtc tccgaagagg ccgagcgcgt tgcaggccgc
     3601 gaccgcgtcg gccgccgggg tcgtgtcggc ggagaggagc tccacgctct cggccgggaa
     3661 acggctccgg agccgcgcga gggcgcggcg gatcttgggg cggtcgccgc cgaggaggag
     3721 gtaggcgggg cgaagctcgt cggcggccac ggcgcgagca tagtcagccg cctacccgga
     3781 cggtgaggcg cgagccgtcg gactcgacga cgacgtcgcc gtcccggtcg gtgcggtaca
     3841 cggcgacgcc cgcctccgcg tcgagcgccg cgatcgtcga cggcgccggg tgcccgtacg
     3901 agttcgaccg cccgacggag atgacggcga cccgcgggcg cgtctcgtcg aggagcgccg
     3961 gcagccgctc gtcggccgac ccgtgatgcg ggaccttcag cacctcgatc tccgggaggc
     4021 gcagcgggag gagaacgtgc gattcggcgt cggcggtgag caggacgtcg gtcgtgccgt
     4081 aggtggccag gaggacgatc gcccgatcgt tcgggtcgcc gccgggcgga ccggggccgt
     4141 cgggccagag gacttcgagc tcgaggcgcc cgagccggta gcggcggccg gccccggcgg
     4201 tgacgaccgg gacgccctgc tcggcggccg cggccagcgc ctcgtcccgc tcctcgctcg
     4261 tgcccgcgag acccgggtcc aggacgaacc cgacgtccac gcgctcgagg acctcagcgg
     4321 cgccgccgac gtggtcgcgc tgcgggtggg tgaggacgag cgcatccagc cggcggatgc
     4381 cgagcgccga gagctcgtcc gcgac
//
LOCUS       DASTFC010000255        10870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_978303,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000255 DASTFC010000000
VERSION     DASTFC010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978303"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /gene="egtD"
                     /locus_tag="VFO88_07635"
     CDS             complement(<1..807)
                     /gene="egtD"
                     /locus_tag="VFO88_07635"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET9545433.1"
                     /translation="MTEVRVAPPLVDVLLGPAERRAALERDVREGLTATPKKLPPKWH
                     YDAEGSRLFGEITRLPEYYLTRAEREILAARAGDIAAVSRADTLVELGAGTSEKTRLL
                     LDALREAGRLERIVVLDVDEETLRESAERLAREYPGVAVHGVAGDLEQHLSELPHEGR
                     RLIAFLGSSIGNFETAERAAFLRRLRGAIDPGESFLLGTDLVKDPARLHAAYDDAAGV
                     TPRFSLNLLSVLNRELGADFDLARFRHVPRWDAERELVDIRLRSLGAQRVC"
     gene            complement(804..1898)
                     /locus_tag="VFO88_07640"
     CDS             complement(804..1898)
                     /locus_tag="VFO88_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9545434.1"
                     /translation="MTVASRDQRESVAADLADAREATLRLLEPLDDDALRAQWSPLQS
                     PLVWDLAHIGHFEDLWIAQRVGGREPLLAEGEELYDAFKHARAERDRLPLLDPAAARD
                     FLARVRERTLAVLSDVDLENGDELVRGGYAFWLVLEHERQHEETMLQTIALSGLEHPG
                     GGPAAVAGEGGDILVEAGPFTLGTDAPLAYDNEQPAHERELAAFRIDAEPVTVGAYRA
                     FLAEHPDVEAPLGWNGDGTVRRFGRDAPLDDAEPVQHVSFHEAEAFARGAGRRLPTEA
                     EWEKAAKAGVLRSAGEVWEWTSSPFAGYPGFRAFPYAEYSEVFFGDEYRVLRGGSWAT
                     HPSVARVTFRNWDFPIRKQIFAGFRLAGEP"
     gene            1965..4664
                     /locus_tag="VFO88_07645"
     CDS             1965..4664
                     /locus_tag="VFO88_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9545435.1"
                     /translation="MATVSPLWSIDPDEALTRLDATEHGLTSAEAEPRLARHGPNELE
                     EEQRVSRLRLLLHQFQSPLIFILLVATAVTLALGEFVDAAVIAAVLLVNAAVGFSQES
                     RAAQAIEALRRLARARARVVRDGREQELDARVLVPGDVLLVEAGARVAADCRILHAVA
                     LEVDESLLTGESTTVAKAAEPVPAGAALGDRASMLFTGTTVTRGHCRAVVAATGADTE
                     LGRIAGSMREVAEPPTPLQRRMDRFAHLVGIAVLAVSALGFVVGVAAGEDLSELFLAI
                     VALAVAAIPEGLPIVMTLVLAVSVRRMARRRAIVRRLPAVETLGSCTVIGSDKTGTLT
                     QNRMTVERILAGGEELHVTGGGYAVAGELRLDGRPVELEPGSPAHEALIAGALCNDAS
                     AVPAGEEFHVRGDPTEIALVVAAAKAGLYKDELEERLVRVAEIPFDPSTRFAVTCNIG
                     EDGAVAFVKGAPEELLARCSDAAGRPLDREAIAEDAHRLAGEGLRVLATASRRLPDRK
                     AAHELAATGAVHGLEFLGLLGMRDPPRDDVPAAIARCEAAGIRVLMVTGDHAATAVAI
                     ARELGIPGAERGAVTGTELDALDETALRGRVREISVFARVSPEHKLRIVQALQANGET
                     VAVTGDGVNDAPALKAADIGAAMGASGTDVAKEASDMIVTDDSFTSIAAAVEEGRIAF
                     DNIRKATFFLISSGAATILAVLASIAFRFQLPFFPAQLLWLNLVTNGIQDVALAFEPG
                     EQDVLDRPPRPRREGIVSRLLWERTLIAGVVMAAGTVGLFLWELEATDDLGHARTVAL
                     TTMVLFQVVHVGNSRSEHRSAFAKSPFSNRFLFVGTVVSLGVHAAALYLPPTQYVLRV
                     EPLDLGTWVTMAVVSLSVLVAVELHKALRGANEQPSLRRSTMV"
     gene            4664..5137
                     /locus_tag="VFO88_07650"
     CDS             4664..5137
                     /locus_tag="VFO88_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9545436.1"
                     /translation="MTNDAGDRALSLLFRIYTSGEACETLVDETVDPHGVADGFVTMS
                     LLSRAGTLTPTEISRRLGMRLTTTSALIRRLVERGHARQIPNPRDGRSQLVTLTEEGE
                     QALTAAFPAFFETIRDVESHLTHSTEEIQAALDDLEQAFRAVREERLERARATSA"
     gene            5702..8293
                     /locus_tag="VFO88_07655"
     CDS             5702..8293
                     /locus_tag="VFO88_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9545437.1"
                     /translation="MARATLTTRRLTRLVLLALVALVATLATTGTAGAAINPLGPPWC
                     GEPMNDAAGNLPDGSSPTHPVGSFPHIPYYAIKCTLDEIQALSNGRMEIETIGESALG
                     RPLYLVTFNVTDTDEQRKAFLNWREIRKDAVDNPARAQEKLAAFGDDVKIPVYIQGAI
                     HGNEYEGVESNFELIYKLATTPYGEDPLVDKVLDNAIVLFNVIQNPDGRIAGTRANGN
                     GFDLNRDFMTQSQSETKASISVMQRYLPPEMLDLHGYVSPMLIEATTLPHNPSIEYDL
                     WLKWNQPRTWENKLDLMAEGNFDSQRPINEWCQDDFPSASCPTAGPDFAEGWDDWGPF
                     YTPMYLQHFGLDSSTTEMCNSTGTICGDGTHQRGRLGSRMQQEIVSWSTLEFAIDNKV
                     EMLHDMLERNVRGKTDAPRPDCCEVTDFIPTANSADKQNWMTDYPKAFVIPLGEGQRS
                     DAEANRLAQFLLDNAIEVHELKQDATLGDQTFQRGSYVVWMTQAFRGFAGTALGIGDD
                     VSSQISQLYAPPASWSHGYLWGADVVTVPDGADFGPLTNRILKPKHLGGGVEPGRASA
                     YALEIDSATAVRVLNGLLDEGVAAQVATTETGGLPAGSVFFGADPATTARLDSAGKGN
                     GLTFRAANGSISGLEPIERKPRIAVLVGSVDQQTWVLRNLGFDANPVSTATINSAADD
                     PLAEYDIVFNTGNWPGATQQTARDRLTAFFARGGGYIGAGSASGTFLQTGGQIAGYAA
                     ATRSGAGRSGIVNWINQGGSSSPIVGTYPANDTAIMDPPTWFTSVPGGFAVDGRFPAS
                     GILASGFWLTSDAQSASAPNSAVIVHGENIAGTARATLFAMNPLYRADPERMWPAVGA
                     AAYWAQQ"
     gene            8425..10284
                     /locus_tag="VFO88_07660"
     CDS             8425..10284
                     /locus_tag="VFO88_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9545438.1"
                     /translation="MRTRLDSLHGGRLALGVAALLALLGGGVLHLAGLPRAGDAVWAG
                     SVAVMLVPLTLSVARTLLRRDVGVDAIALVAMASALALGEYLAGAVVALMLAGGNALE
                     EYAAGHARRELTRLLERMPRIAHVRRGGEILEVPVGEVEPGEVVVVRAGEIVPVDGVV
                     ARGAATVDESALTGEPLPVTRFEGAEVRSGSSNAADAFDLRATRRAAESAYAGIVRLV
                     REAQAQRAPFVRMADRYAAIFLPVALVVAGGAWAISDDPVRFLAVMVVATPCPLILAA
                     PIALLAGVSRAARRGVIVKGSGVIERLGRAQSVLLDKTGTVTLGAPALERVVAFDGTS
                     EDDVLRLAASVDQLSAHVLAEALVRAAEDRALRLAAPEDVVEGRGQGIEGRVDGRRVT
                     VGSAAWLAQRGYGGADAAARSLDGTVAPGLATILVGVEGAVAGAIVMGDRLRDDASGL
                     VDGLREQGIRHVALATGDRRAVGEAVGATLGVDRTYAELDPEDKMAIVRELRADPSLV
                     PVVMVGDGINDAPALARADVGIALGAGGATVASQTADAVIIQDRVDRVVDAVAIGRRS
                     LGIARQSVLAGMGLSLVAMGFAAGGYIAPVAGALIQEGIDVAVILNALRALRD"
     gene            10350..>10870
                     /locus_tag="VFO88_07665"
     CDS             10350..>10870
                     /locus_tag="VFO88_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545439.1"
                     /translation="MTFGLAEVRVRGFRSARDVTLSPGDVCALVGEASSGKSNLLEAI
                     RLLLDPAAPEPGPDDVWRGDGGVRITGRLGDGREIAYELGRPRRLPEGDMVPAALFLP
                     AALRSETVVAPSAPATEAAAEAERRFAAALADRDGSAAAPALAFVAGLERWCEAGLSG
                     IVLLVEEPELFLRP"
BASE COUNT         1450 a         3994 c         3982 g         1444 t
ORIGIN      
        1 gcacacgcgc tgcgccccga gcgagcgcag gcggatgtcg acgagctccc gctcggcgtc
       61 ccagcgcggg acgtgccgga agcgggcgag gtcgaagtcc gcgccgagct cgcggttcag
      121 gacggagagc aggttgagcg agaaccgcgg cgtgacgccg gccgcgtcgt cgtaggcggc
      181 gtggagccgc gccgggtcct tcacgaggtc cgtcccgagc aggaacgact cgccggggtc
      241 gatcgcgccg cggagccggc ggagaaacgc cgcccgctcg gccgtctcga agttcccgat
      301 gctcgagccg aggaaggcga tgaggcgccg cccctcgtgc ggcagctcgg agaggtgctg
      361 ctcgaggtcg ccggcgaccc cgtggacggc gacgcccggg tactcgcgcg cgagccgctc
      421 ggcgctctcg cggagcgtct cctcgtccac gtcgaggacg acgatccgct ccagccgccc
      481 cgcctcgcgg agcgcgtcga gcaggagccg cgtcttctcg gacgtgcctg ccccgagctc
      541 gacgagcgtg tcggcgcgcg agacggccgc gatgtcgccc gcgcgcgccg cgaggatctc
      601 gcgctccgcg cgcgtgaggt agtactccgg caatctcgtg atctcgccga agagccgcga
      661 gccctcggcg tcgtagtgcc acttcggcgg cagcttcttc ggcgtcgccg tgagcccctc
      721 gcggacgtcg cgctcgagcg ccgcgcggcg ttccgccggc ccgagcagga cgtcgacgag
      781 cggcggggcg acgcgaacct cggtcatggc tcacccgcca gccggaagcc cgcgaagatc
      841 tgcttccgga tcgggaagtc ccagttgcga aacgtcaccc gcgcgacgct cgggtgcgtc
      901 gcccacgacc cgccgcggag gacgcggtac tcgtcgccga agaagacctc cgagtactcg
      961 gcgtacggga aggcgcggaa gcccgggtag ccggcgaagg gcgacgacgt ccactcccac
     1021 acctcgccgg ccgagcgcag gacgcctgcc ttcgccgcct tctcccattc ggcctcggtc
     1081 ggcaggcgcc ggcccgcccc gcgcgcgaag gcctccgcct cgtggaacga gacgtgctgc
     1141 accggctcgg cgtcgtcgag cggagcgtcg cggccgaacc ggcgcaccgt cccgtccccg
     1201 ttccagccga gcggcgcctc gacgtccgga tgctccgcga gaaacgcgcg gtacgcgccg
     1261 acggtgaccg gctccgcgtc gatccggaac gcggcgagct cgcgctcgtg agccggctgc
     1321 tcgttgtcgt acgcgagcgg cgcatcggtg ccgagcgtga acggccccgc ctcgacgagg
     1381 atgtcgccgc cctctcccgc gacggcggcc ggcccgccgc ccgggtgctc gagccccgag
     1441 agcgcgatcg tctggagcat cgtctcctcg tgctggcgct cgtgctcgag cacgagccag
     1501 aacgcgtagc ccccgcggac gagctcgtca ccgttctcga ggtcgacgtc cgagagcact
     1561 gcgagcgtcc gctcgcgcac gcgcgcgagg aagtcccgcg cggcggccgg gtcgaggagc
     1621 ggcagccggt cgcgctccgc ccgcgcgtgc ttgaacgcgt cgtagagctc ctccccctcg
     1681 gcgaggagcg gctcgcgccc gccgacgcgc tgcgcgatcc acaggtcctc gaagtgcccg
     1741 atgtgcgcga ggtcccagac gagcggcgac tggagcggcg accactgcgc ccggagcgcg
     1801 tcgtcgtcga gcggctcgag gaggcggagc gtcgcctcgc gcgcgtccgc gagatccgcg
     1861 gcaacgctct cccgttggtc tctcgatgcg acggtcatgc gcggcgcccc gccgcagcgt
     1921 actgactgga gccggggtcg cggggctctg caaggctggc cgcgatggca acggtgtccc
     1981 cactctggtc gatcgatccg gacgaggcgc tcacgcggct cgacgcgacc gagcacgggc
     2041 tcacgagcgc cgaggcggag ccgcggctcg cgcgccacgg gccgaacgag ctcgaggagg
     2101 agcagcgggt gtcgcggctc cgcctgctcc tgcaccaatt ccagagcccg ctcatcttca
     2161 tcctcctcgt cgcgacggcg gtgacgctcg cgctcggcga gttcgtggac gccgcggtga
     2221 tcgcggccgt gctcctcgtg aacgccgccg tcggcttctc gcaggagtcg cgcgccgcgc
     2281 aggcgatcga ggcgctccgc cggctcgccc gggcgcgggc gcgcgtcgtc cgcgacggac
     2341 gcgagcagga gctcgacgcg cgcgtgctcg tccccggcga cgtgctgctc gtcgaggcgg
     2401 gcgcgcgggt cgcggcggac tgccgcatcc tccacgcggt cgcgctcgag gtcgacgagt
     2461 cgctcctgac gggcgagtcg acgacggtgg ccaaggcggc cgagcccgtg ccggccggcg
     2521 ccgcgctcgg cgaccgggcg agcatgctct tcacggggac gacggtcacg cgcggccact
     2581 gccgcgcggt ggtcgcggcg acgggcgcgg acaccgagct cggccggatc gccggaagca
     2641 tgcgcgaggt cgcggagccg ccgacgccgc tccagcgccg gatggaccgc ttcgcccacc
     2701 tcgtcgggat cgccgtcctc gccgtctcgg cgctcggctt cgtcgtcggc gtggccgccg
     2761 gcgaggacct gtcggagctg ttcctcgcca tcgtcgccct cgccgtggcg gcgatcccgg
     2821 aggggctccc gatcgtcatg accctggtac tcgccgtgag cgtgcgccgg atggcgcggc
     2881 ggcgcgcgat cgtccggcgg ctccccgcgg tcgagacgct cgggagctgc acggtgatcg
     2941 gatccgacaa gaccggcacg ctgacgcaga accggatgac ggtcgaacgg atcctcgcgg
     3001 gcggcgagga gctccacgtc acgggcggcg gctacgcggt ggccggtgag ctgcgcctcg
     3061 acgggcggcc ggtcgagctc gagcccggga gccctgcgca cgaggcgctc atcgccggcg
     3121 cgctctgcaa cgacgcgagc gccgtcccgg ccggcgagga gttccacgtt cgcggcgacc
     3181 cgaccgagat cgcgctcgtc gtcgccgccg cgaaggccgg cctctacaag gacgagctcg
     3241 aggagcggct cgtccgggtc gcggagatcc ccttcgaccc gtcgacgcgc ttcgcggtca
     3301 cgtgcaacat cggcgaggac ggagccgtcg cgttcgtgaa gggcgcgccg gaggagttgc
     3361 tcgcacgctg cagcgacgcc gccggccggc cgctcgaccg cgaggcgatc gccgaggacg
     3421 cccacaggct cgccggcgag gggctgcgcg tgctcgccac ggcgtcgcgc cggctgccgg
     3481 atcgcaaggc ggcgcacgag ctcgccgcga ccggcgccgt ccacggcctc gagttcctcg
     3541 ggctgctcgg catgcgcgac ccgccgcgcg acgacgtgcc ggccgcgatc gcgcgctgcg
     3601 aggcggcggg catccgcgtg ctcatggtca cgggtgacca tgcggcgacc gccgtcgcga
     3661 tcgcgcgcga gctcgggatc ccgggtgccg agcgcggcgc cgtgacggga acggagctcg
     3721 acgcgctcga cgaaaccgcc ctgcggggcc gcgtgcggga gatctcggtc ttcgcgcgcg
     3781 tctcgcccga gcacaagctc cgcatcgtcc aggccctcca ggcgaacggc gagaccgtcg
     3841 cggtgaccgg cgacggcgtg aacgacgccc cggcgctgaa ggcggccgat atcggcgcgg
     3901 cgatgggcgc ctcgggcacg gacgtcgcca aggaggcgtc cgacatgatc gtgacggacg
     3961 acagcttcac gagcatcgcg gccgcggtcg aggaaggccg gatcgccttc gacaacatcc
     4021 gcaaggcgac gttcttcctc atctcgagcg gcgccgccac gatcctcgcc gtgctcgcga
     4081 gcatcgcgtt ccgcttccag ctcccgttct tcccggcgca gctcctctgg ctgaacctcg
     4141 tgacgaacgg gatccaggac gtcgcgctcg cgttcgagcc gggcgagcag gacgtgctcg
     4201 accggccgcc gcgtcctcgc cgggagggga tcgtctcgcg cctgctctgg gagcggacgc
     4261 tgatcgccgg cgtcgtcatg gcggccggga cggtcggcct cttcctgtgg gagctcgagg
     4321 cgacggacga cctcggccac gcccgcaccg tggcgctgac gacgatggtg ctcttccagg
     4381 tcgtccacgt cgggaactcg cgctcggagc accgctcggc cttcgcgaag agcccgttct
     4441 cgaatcgctt tctcttcgta gggacggtcg tgtcgctcgg cgtccacgcg gccgcgctct
     4501 acctgccgcc gacgcagtac gtgctgcgcg tggagccgct cgacctcggc acgtgggtga
     4561 cgatggcggt cgtctcgctc agcgtgctcg tcgcggtcga gctccacaag gcgctccgcg
     4621 gggcgaacga gcagccgtcc ctgcgccgtt ctacgatggt gtgatgacaa atgacgccgg
     4681 cgaccgcgcg ctgagcctcc tgttccgcat ctacacgtcc ggcgaggcgt gcgagacgct
     4741 cgtcgacgag acggtcgacc cgcacggcgt cgcggacggg ttcgtgacga tgagcctcct
     4801 cagccgggcc ggcacgctga cgccgacgga gatctcccgg cggctcggga tgcggctgac
     4861 gacgacctcg gcgctcatcc ggcgcctcgt cgagcggggg cacgcgcggc agatcccgaa
     4921 tccgcgcgac gggcgctcgc agctcgtcac gctgaccgag gagggcgagc aggcgctcac
     4981 cgccgcgttc ccggccttct tcgagacgat ccgcgacgtc gagagccacc tcacgcactc
     5041 gaccgaggag atccaggccg cgctcgacga cctcgagcag gcgttccgcg ccgtgcgcga
     5101 ggagcgcctc gaacgggcgc gcgccacgtc ggcgtagtcg ggccggacgg gtagacgacc
     5161 cgccggccgg aatgagggac ggccggcggg tcgtccgcca cactgcacgc ggatcggccc
     5221 cgggagggtt ctcgggcggc ttcgcccggc ccccgcgcgc aggcttcggc cgcgaggcta
     5281 ggcggccacc tcgcgggccg gcaccgcgtt cccgcgcggg ccggctggga tcgggtggag
     5341 caggatgtgg aggtctcggt tcattccggg cgtctccaga tgtccgcgcg taccgcagga
     5401 atgcacgaac tggtgcagta ttgcatcaac caccgggccg cgcaagcgag ggctcttcgc
     5461 accggcgggc gaacgagacg gaccttgact tgccgtgccg actgtctgag aatcgtcggg
     5521 tggcacatgc ggcgagggga ggccgcgtgc ggggtcgggc gacgcagtcc ggatgtccca
     5581 cgctcgttct ccgtcagcgc gtcggttcgt cgttcggagc cggtgaccgg cttccgtccg
     5641 acagcgcgct gaggcccacc gacccttccc gcgcatatcc gattccccgc taggaggccc
     5701 tttggcacgc gcaacgctca caacacgacg cctcaccagg ctcgtcctgc tcgcgctcgt
     5761 cgcgctcgtc gcgacgctcg cgacgacggg caccgccggt gcggcgatca acccgctcgg
     5821 cccgccgtgg tgcggcgagc cgatgaacga cgccgccggc aacctgccgg acggctcgag
     5881 cccgacccat ccggtcggca gcttcccgca catcccgtac tacgcgatca agtgcacgct
     5941 ggacgagatc caggcgcttt cgaacgggcg catggagatc gagacgatcg gcgagtccgc
     6001 gctcggccgc ccgctctacc tcgtgacgtt caacgtcacg gacacggacg agcagcgcaa
     6061 ggcgttcctc aactggcgcg agatccgcaa ggacgccgtc gacaacccgg cgcgggcgca
     6121 ggagaagctc gccgcgttcg gtgacgacgt gaagatcccc gtctacatcc agggcgcgat
     6181 ccacgggaac gagtacgagg gcgtcgagtc gaacttcgag ctgatctaca agctcgcgac
     6241 gacgccgtac ggcgaggatc cgctcgtcga caaggtgctc gacaacgcga tcgtcctctt
     6301 caacgtgatc cagaaccccg acggccggat cgcgggcacg cgcgcgaacg gcaacgggtt
     6361 cgacctgaac cgcgacttca tgacgcagtc gcagtcggag acgaaggcgt cgatctcggt
     6421 catgcagcgg tacctcccgc ccgagatgct cgacctgcac gggtacgtct ccccgatgct
     6481 gatcgaggcg acgacgctcc cgcacaaccc gtcgatcgag tacgacctct ggctcaagtg
     6541 gaaccagccg cgcacgtggg agaacaagct cgatctcatg gcggagggca acttcgacag
     6601 ccagcggccg atcaacgagt ggtgccagga cgacttcccg tccgcgagct gcccgacggc
     6661 cggcccggac ttcgcggagg gctgggacga ctggggcccc ttctacaccc cgatgtacct
     6721 ccagcacttc ggcctcgaca gctcgacgac cgagatgtgc aactcgaccg gcacgatctg
     6781 cggtgacggc acgcaccagc gcggccgcct cggctctcgg atgcagcagg agatcgtcag
     6841 ctggtcgacg ctcgagttcg cgatcgacaa caaggtcgag atgctccacg acatgctcga
     6901 gcggaacgtc cgcggcaaga ccgatgcccc gcggccggac tgctgcgagg tcacggactt
     6961 catcccgacc gcgaactcgg cggacaagca gaactggatg accgactacc cgaaggcgtt
     7021 cgtgatcccg ctcggcgagg gccagcgctc cgacgccgag gcgaaccggc tcgcccagtt
     7081 cctcctcgac aacgcgatcg aggtgcacga gctgaagcag gacgcgacgc tcggcgacca
     7141 gaccttccag cgcggctcgt acgtcgtctg gatgacgcag gcgttccgcg ggttcgccgg
     7201 caccgcgctc gggatcggcg acgacgtgtc cagccagatc agccagctct acgcgccgcc
     7261 ggcgtcgtgg agccacggct acctctgggg cgcggacgtc gtcacggttc ccgacggcgc
     7321 cgacttcggc ccgctcacga accgcatcct caagccgaag cacctcggcg gcggcgtcga
     7381 gcccggcagg gcgtcggcct acgccctcga gatcgactcg gcgacggcgg tgcgggtcct
     7441 gaacggcctc ctggacgagg gcgtcgcggc gcaggtcgcg acgaccgaga cgggcggcct
     7501 gccggccggg agcgtgttct tcggcgcaga tccggccacg acggcccgcc tcgactcggc
     7561 cggcaagggg aacgggctca cgttccgggc cgcgaacggc tcgatcagcg gcctcgagcc
     7621 gatcgagcgc aagccgcgga tcgccgtgct ggtcggctcg gtcgaccagc agacgtgggt
     7681 cctgcggaac ctcggcttcg atgcgaaccc ggtctcgact gcgacgatca actcggctgc
     7741 ggacgatccg ctggccgagt acgacatcgt gttcaacacg ggcaactggc cgggtgcgac
     7801 ccagcagacg gcccgcgacc ggctgacggc gttcttcgcc cgcggcggcg gctacatcgg
     7861 cgccggctcc gcgagcggga cgttcctcca gacgggcggc cagatcgccg gctacgcggc
     7921 ggcgacccgg agtggtgcgg gccgcagcgg catcgtgaac tggatcaacc agggcggctc
     7981 gtcgagcccg atcgtcggga cctacccggc gaacgacacg gcgatcatgg atccgcccac
     8041 gtggttcacg tccgtgccgg gcgggttcgc ggtcgacggc cgcttcccgg cgtcggggat
     8101 cctcgcctcc gggttctggc tcacgagcga cgcccagtcg gcgtcggccc cgaactcggc
     8161 cgtgatcgtc cacggcgaga acatcgccgg caccgcccgt gccacgctct tcgcgatgaa
     8221 cccgctctac cgggcggatc cggagcgcat gtggccggcg gtcggcgctg cggcctactg
     8281 ggcgcagcag tagcgcagga aaacaacgcg acgaaacgcg agaggggcgg ggtgacccgc
     8341 ccctctcgct cgtagtaggg agccgcaagg ccgctccgcg attccgccga tgcgcgtacg
     8401 gcacgttcgc gcaatgcttg gagcatgcgg acgcggctcg attcgctcca cggcggccgc
     8461 ctggccctcg gcgtcgcggc cctcctcgcg ctgctcggcg gcggcgtcct ccacctcgcc
     8521 gggctgcccc gcgcgggcga cgcggtctgg gccggctccg tcgccgtcat gctcgtgccg
     8581 ctcaccctct ccgtcgcgcg gacgctcctg cggcgcgacg tcggcgtcga cgcgatcgcg
     8641 ctcgtcgcga tggcctccgc gctcgcgctc ggggagtacc tcgcgggcgc ggtcgtcgcg
     8701 ctcatgctcg ccggagggaa cgcgctcgag gagtacgcgg ccgggcatgc acgccgggag
     8761 ctcacgcggc tgctcgagcg gatgccccgg atcgcccacg tccggcgcgg cggcgagatc
     8821 ctcgaggttc ccgtggggga ggtcgagccg ggcgaggtcg tcgtcgtgcg cgcgggcgag
     8881 atcgtcccgg tcgacggcgt cgtcgcgcgc ggcgcggcga ccgtcgacga gtcggcgctc
     8941 acgggcgagc cgctcccggt cacgcggttc gagggcgccg aggtccgcag cggctcgtcg
     9001 aacgcggccg acgcgttcga cctgcgcgcg acccgacgcg cggccgagag cgcgtacgcg
     9061 gggatcgtcc gcctcgtgcg cgaggcacag gcgcagcgcg cgcccttcgt gcgcatggcc
     9121 gaccgctacg cggcgatctt cctgccggtc gccctcgtcg tcgccggcgg cgcgtgggcg
     9181 atctcggacg atcccgtccg cttcctcgcc gtcatggtcg tcgcgacccc gtgccccctc
     9241 atcctggccg cgccgatcgc gctcctcgcg ggcgtgtcgc gcgcggcgcg gcgcggcgtg
     9301 atcgtgaagg gcagcggcgt gatcgagcgg ctcgggcgcg cgcagagcgt cctcctcgac
     9361 aagaccggca cggtcacgct cggggcgccg gcgctcgagc gcgtggttgc attcgacggg
     9421 acgagcgaag acgacgtcct gcgcctcgcc gcctcggtcg accagctctc cgcccacgtg
     9481 ctcgccgagg cgctcgtccg tgccgccgag gaccgggccc tgcgactcgc cgcgccggag
     9541 gacgtcgtcg agggacgcgg gcagggcatc gaaggccgcg tcgacgggcg gcgcgtcacc
     9601 gtcggcagcg ccgcgtggct cgcccagcgc ggctacggcg gcgcggacgc cgccgcccgc
     9661 tcgctcgacg gcaccgtcgc ccccggcctc gccacgatcc tcgtcggcgt cgagggcgcg
     9721 gtggcgggcg cgatcgtgat gggcgaccgc ctgcgcgacg acgcgtccgg gctcgtcgac
     9781 gggctgcgtg aacaggggat ccgccacgtc gcgctcgcga ccggcgaccg gcgcgcggtc
     9841 ggcgaggcgg tcggcgcgac gctcggcgtc gacaggacgt acgcggagct cgatcccgag
     9901 gacaagatgg cgatcgtccg cgagctgcgc gccgacccga gcctcgtccc ggtcgtcatg
     9961 gtcggcgacg ggatcaacga cgcccccgcg ctcgcgcggg cggacgtcgg aatcgccctc
    10021 ggcgcgggcg gggcgaccgt cgcgtcgcag acggcggacg cggtcatcat ccaggaccgg
    10081 gtcgaccgcg tcgtcgacgc ggtcgcgatc ggccgccgct cgctcggcat cgcacggcag
    10141 agcgtcctcg cggggatggg gctctcgctc gtcgcgatgg gcttcgccgc gggcggctac
    10201 atcgccccgg tcgcgggtgc gctcatccag gagggcatcg acgtggccgt catcttgaac
    10261 gctctgcgcg ccctccgcga ctagcgtgcc gcacggccgg cccgagtccg tcccggcgga
    10321 ccccggagcc cggacatagg ctggcggccg tgacgttcgg actggcggag gtgcgcgtgc
    10381 ggggcttccg ctcggcgcgc gacgtgacgc tctcgccggg agacgtctgc gcgctcgtgg
    10441 gcgaggcgag ctcgggcaag tcgaacctcc tcgaggcgat ccggctcctg ctcgacccgg
    10501 cggcacccga gccgggcccg gacgacgtct ggcggggcga cggcggcgtg cggatcacgg
    10561 gccgcctcgg cgacgggcgg gagatcgcgt acgagctcgg gcgcccgagg cggctgcccg
    10621 aaggcgacat ggttcccgcc gcgctcttcc tgccggcggc gctccgcagc gagacggtcg
    10681 tggcgccctc cgcgcccgca accgaggcgg ccgcagaggc ggagcggcgc ttcgccgccg
    10741 cgctcgccga ccgcgacggg tcggccgccg cgccggcgct cgccttcgtg gccggcctcg
    10801 agcggtggtg cgaggcaggg ctctcgggca tcgtgctcct cgtggaggag ccggagctct
    10861 tcctgcgccc
//
LOCUS       DASTFC010000256         6175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_467527,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000256 DASTFC010000000
VERSION     DASTFC010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467527"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VFO88_07670"
     CDS             <1..1321
                     /locus_tag="VFO88_07670"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9545440.1"
                     /translation="GAPLLALLAVLFVHRPAVLVPAVVGTAWLRWPFEIDTTDGLSIG
                     LGEAGSLGRLVPLYAVVAAAGLALLWRLARGEEPRVLPAVLAVPAALLSGLTILSLVW
                     AYDPAAAEDRLAFFVLPFVCVLGLVARAPWRPWLPRVLAVEAVALAVLFAAVGLVESW
                     TRTLIFYDPKVAVANEYTSYFRVTSLFSDPSIYARHVALAIVVLVVCLLLGRIGVLAG
                     AALIAFLFAGIYVSYSQSTMVALAAAVCLVTFLVADARTRRLITVTAAVLVVAGVAAF
                     GALVAAGYDPERLTSTRSTLVADTWRVFETHPVEGVGLASQPAASRDLTDGPRSARRN
                     VSHTAPLTVAAELGIVGVALYAAFIAGALLLLLRVRARDEALGLALLGVATVLFVHSL
                     SYGVFFDDPLVWAVLGIGAASGPSEAVFRAPAALRRRRRPSPAPAAR"
     gene            1336..2718
                     /locus_tag="VFO88_07675"
     CDS             1336..2718
                     /locus_tag="VFO88_07675"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET9545441.1"
                     /translation="MRADTADTNERGLAPRDAWLLLGTLGLVVLVSVPLLGAEPWPFR
                     PDRVDPTGPFAAFVRATDGEWDTEALRAPGLLAGVLVALAAAAVPLRAAWPRWAAVAL
                     TAIVVCLLAVPAVVLQAGLRQSTEPWFHTNDSTFQIELAGELLRNGENPYGHDYSQSG
                     MERFYSLDGSATDETREEQVALRHFAYFPGTVLTAAVWTLLPEPFSDYRFFVLLATLA
                     GLAVALAFPGPLAWRLAGGALIAASPLALRAAWFGTADAPSLALTVLAFALVARRRFL
                     WAAAALGGAVLLKQFALVALPFLALWAWRQAGRREALRAGGVFAAVVAVGVLPFAIWD
                     PGALWADTIEYGGSTYRIVGYGLSGLLLEAGLLESRTGGYPFLLLLALVWLPVTAWLL
                     WTWRRAEELWPAAVGFAVSMFLLVFLARVFHGSYLVWPLAGIVVAALLAAPQRVPSAR
                     SHVPGMPPRT"
     gene            complement(2664..3788)
                     /locus_tag="VFO88_07680"
     CDS             complement(2664..3788)
                     /locus_tag="VFO88_07680"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9545442.1"
                     /translation="MIVIVVLFWATFAALVWTHLGYPLAAALLARLRPRPVAREDWTP
                     SVTLIVPAHDEEDVIAGRLENLLAQDYPPERLEIIVGSDASTDRTDAIVEEIAAREPR
                     VRLVRCPRGGKLATLNRLHTEGTGEVLAFTDANTRWEPDALRKLVRSLADPEVGYVTG
                     KLVLRDADGTNREGLYWRYELWLREQESALGSITGGNGAIYALRRETYIPQPYGQDAA
                     LPAFLAGKGLRAVYDTEAIAYEKPARDLEDEFARKARMFRWAWHLLFAGRPTRGAGRL
                     FRFQWFSHRTLRYASGIIHLTFLLATTALLLDGTVYVVAWVLQLAWLALAGLAWLRVP
                     LPGAGVAYYYLLVTLATIVGAVRYVRGGIPGTWERAEGTR"
     gene            complement(3785..4825)
                     /locus_tag="VFO88_07685"
     CDS             complement(3785..4825)
                     /locus_tag="VFO88_07685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9545443.1"
                     /translation="MGAAGAGRLREVEPAAWDGLLDGLGCRDAYFRRGFFDAARLVEP
                     GAATLLHLEGAGGHVVFACIVREVPAGHGARDVVTPYGYGGPLALGADPPVARFHELY
                     EEWCAAEGVVTTFVRFHPLHENWRYAAPRARLERLANTASWPLRPDADLLGEMHAKHR
                     REIRRGVEAGLETVVRPAPEELGDFVALYEDTMRRRDADAFYFFSAAYWDALAAGLRE
                     RLLVVDGRFDGELVASALFLATTPWLHYHLGAMRDPGGSLGATKVVLYEAARWAREQG
                     YVELHLGSGLGGREDSLWRFKQRFSPSPGREFWIGKLVHDEARYRELAGDAPTDGYFP
                     AYRAAAAVEARR"
     gene            complement(4827..5987)
                     /locus_tag="VFO88_07690"
     CDS             complement(4827..5987)
                     /locus_tag="VFO88_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545444.1"
                     /translation="MSGPRVAVLGLLWAGLSLARSLGRAGVDVSGIVIHEHEFGLRSR
                     YLTRRAEVLDADERRRDERVLELLRKLAAAGPLFIVPERDDHVELVLRNWDEITALGT
                     IPLPSDPDVVHRLRRKELVLAEAERAGVPAPQTIAPEHEGDIRSADLVLPLVVKPVEG
                     QHFAMAFGRKVLPAATVDEAVDAWRWARERGFDMVVQEHVPDSHEKIFSLFTYIDRSG
                     VARANVVGRKIRQGPVRFGTSAVFETRDEPRVLEAGLRLLESVGYRGFGHVEFAHDAR
                     DDSFKLLEVNTRVPMWGGIAMTDLFDVARIAYDDAAGRPLPPPRTFTDELVWIFFGKD
                     AWVSAQMALRRELGPLGFLAPYARRPKVRAVFALDDPLPAAASVRYLAAKVV"
     gene            complement(5984..>6175)
                     /locus_tag="VFO88_07695"
     CDS             complement(5984..>6175)
                     /locus_tag="VFO88_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9545445.1"
                     /translation="REDQTWADRFAYDAWYLEHWSLWLDARILLRTVGQLFRPEPVPV
                     VDPLIVERAQRGAAEEREP"
BASE COUNT          699 a         2364 c         2234 g          878 t
ORIGIN      
        1 cggggccccg ctcctcgcgc tcctcgccgt cctgttcgtc caccgccccg cggtgctcgt
       61 gcccgcagtc gtgggcacgg cgtggctgcg ctggccgttc gagatcgaca cgaccgacgg
      121 gctctcgatc gggctcggcg aggccgggtc gctcgggcgg ctcgtgccgc tctacgccgt
      181 cgtcgcggcg gccgggctcg cgctgctctg gcgcctcgcg cggggcgagg agccccgcgt
      241 cctcccggcc gtcctcgccg tgccggccgc gctcctgagc gggctcacga tcctctcgct
      301 cgtctgggcg tacgacccgg ctgccgcgga ggatcggctc gcgttcttcg tcctgccctt
      361 cgtctgcgtg ctcggcctcg tcgcccgcgc gccctggcgg ccgtggctcc cgcgggtgct
      421 cgccgtcgag gcggtggcgc tcgccgtcct cttcgccgcc gtcggcctcg tcgagtcctg
      481 gacgcggacg ctcatcttct acgacccgaa ggtcgcggtc gcgaacgagt acacgagcta
      541 cttccgggtg acgtcgctct tctccgaccc gtcgatctac gcgcggcacg tcgcgctcgc
      601 gatcgtcgtc ctcgtcgtct gcctgctgct cggccggatc ggcgtcctcg cgggagcggc
      661 gctcatcgcg ttcctcttcg cggggatcta cgtctcctac tcccagtcga cgatggtcgc
      721 gctcgccgcc gccgtctgcc tcgtgacctt cctcgtcgcg gacgcgcgga cgcggaggct
      781 catcacggtc accgcggccg tgctcgtcgt cgccggcgtg gccgcgttcg gcgcgctcgt
      841 cgccgcgggc tacgacccgg agcgcctgac gagcacgcgg tcgacgctcg tcgccgacac
      901 ctggcgagtc ttcgagaccc atccggtcga gggcgtcggc ctcgcctcgc agccggcggc
      961 gagccgcgac ctgacggacg gcccacgctc cgcgcggcgc aacgtctcgc acaccgcgcc
     1021 gctcacggtg gccgcggagc tcgggatcgt cggggtcgcg ctctacgcag ccttcatcgc
     1081 cggcgcgctc ctgctcctgc tgcgcgtgcg ggcccgggac gaggcgctcg gcctggcgct
     1141 cctcggcgtc gcgacggtcc tcttcgtcca ttcgctctcg tacggcgtct tcttcgacga
     1201 tccgctcgtc tgggcggtgc tcgggatcgg cgccgcctcc gggcccagtg aggccgtctt
     1261 ccgcgcgccg gccgcgctgc gccgccggcg gcgtccgagc ccggctccgg ccgctcggtg
     1321 aggcaggctg ccgcgatgcg ggcggacacc gccgacacga acgagcgtgg gctcgcaccc
     1381 cgcgacgcgt ggctcctgct cgggacgctg gggctcgtcg tcctggtctc cgtcccgctc
     1441 ctcggcgccg agccctggcc ttttcgcccc gaccgcgtcg acccgaccgg cccgttcgcc
     1501 gcgttcgtcc gcgcgaccga cggggagtgg gacacggagg ccctgcgcgc gcccgggctc
     1561 ctcgcgggcg tcctcgtcgc gctcgcggcg gccgccgtcc cgctccgcgc cgcctggccg
     1621 cgctgggcgg cggtcgcgct cacggcgatc gtcgtctgcc tgctcgcggt gcccgccgtc
     1681 gtcctccagg ccggcctacg ccagtcgacg gagccgtggt tccacacgaa cgactcgacg
     1741 ttccagatcg agctcgccgg agagctgctc cgaaacggcg agaacccgta cggccacgac
     1801 tactcacagt cggggatgga gcggttctac tcgctcgacg gaagcgcgac ggacgagacg
     1861 cgcgaggagc aggtcgcgct gcgccacttc gcctacttcc ccgggacggt tctcaccgcg
     1921 gccgtgtgga cgctcctgcc cgagccgttc tcggactacc gcttcttcgt cctcctggcg
     1981 acgctcgccg ggctcgcggt cgcgctcgcg ttccccggcc cgctcgcgtg gcggctcgcc
     2041 ggcggcgcgc tcatcgcggc gagcccactt gcgctgcgcg ccgcgtggtt cgggaccgcc
     2101 gacgctccga gcctcgcgct caccgtgctc gcgttcgcgc tcgtcgcccg ccgccgcttc
     2161 ctctgggccg cggccgcgct cggcggggcc gtcctcctga agcagttcgc cctcgtcgcg
     2221 ctcccgttcc tcgcgctttg ggcctggcgg caggccggcc ggcgagaggc gctgcgcgcc
     2281 ggcggcgtct tcgcggccgt cgtcgcggtc ggcgtccttc cgttcgcgat ttgggatccc
     2341 ggcgcgctct gggcggacac gatcgagtac ggcggctcca cgtaccggat cgtcggctac
     2401 gggctctccg gcctgctcct cgaggccggg ctcctcgagt cacgcaccgg cggctatccg
     2461 ttcctcctgc tcctcgcgct cgtctggctg ccggtcacgg cctggctcct ctggacgtgg
     2521 cggcgggccg aggagctctg gccggccgcg gtcgggttcg ccgtctcgat gttcctgctc
     2581 gtcttcctcg cccgcgtgtt ccacggctcg tacctcgtct ggccgctcgc ggggatcgtc
     2641 gtcgccgcgc tcctcgccgc gcctcagcgg gtcccctcgg cgcgctccca cgtgccgggg
     2701 atgccgccgc ggacgtagcg caccgcgccg acgatcgtcg ccagcgtgac gagcaggtag
     2761 tagtaggcga cgcctgcgcc ggggagcggg acccggagcc acgcgagccc ggcgagcgcg
     2821 agccaggcga gctggagcac ccatgcgacg acgtagaccg tcccgtcaag gaggagcgcc
     2881 gtcgtcgcga gcaggaacgt gaggtggatg atcccgctcg cgtagcgaag cgtccggtgc
     2941 gagaaccact ggaagcggaa gagccgcccc gcgccgcgcg tcgggcgccc cgcgaagagg
     3001 aggtgccacg cccagcggaa catgcgtgcc ttgcgcgcga actcgtcctc gaggtcgcgg
     3061 gccggcttct cgtaggcgat cgcctcggtg tcgtagaccg cgcgcaggcc cttgccggcg
     3121 aggaaggcgg ggagcgccgc gtcctgcccg tagggctgcg ggatgtacgt ctcgcgccgg
     3181 agcgcgtaga tcgccccgtt cccgcccgtg atcgacccga gcgcggactc ctgctcccgg
     3241 agccagagct cgtagcgcca gtagagcccc tcgcggttcg ttccgtccgc gtcgcgcagc
     3301 acgagcttcc ccgtgacgta gccgacctcc gggtcggcga gcgagcggac gagcttgcgg
     3361 agggcgtccg gctcccagcg cgtgttcgcg tccgtgaacg cgagcacctc gcccgtcccc
     3421 tcggtgtgga gccggttcag ggtcgcgagc ttcccgccgc gcgggcagcg gacgagccgg
     3481 acgcgcggct cgcgcgccgc gatctcctcg acgatcgcgt ccgtccggtc ggtcgaggcg
     3541 tcggacccga cgatgatctc gagcctctcc ggcgggtagt cctgcgcgag gaggttctcc
     3601 aggcggccgg cgatcacgtc ctcctcgtcg tgggcgggga cgatgagcgt cacgctcggc
     3661 gtccagtcct ctctcgccac cgggcgcgga cgcaggcggg cgaggagcgc ggcggcgagg
     3721 ggatagccga ggtgcgtcca gacgagcgcg gcgaacgtgg cccagaagag gacgacgatc
     3781 acgatcaccg gcgcgcctcc acggccgcgg ccgcgcggta ggcggggaag tagccgtcgg
     3841 tcggcgcgtc cccggcgagc tcgcggtagc gcgcctcgtc gtggacgagc ttcccgatcc
     3901 agaactcgcg ccccggcgag ggcgagaagc gctgcttgaa gcgccagagg gagtcctcgc
     3961 gccccccgag cccgctgccg aggtgcagct cgacatagcc ctgctcccgc gcccagcgcg
     4021 ccgcctcgta gaggacgacc ttcgtcgccc cgagcgagcc gcccgggtcg cgcatcgcgc
     4081 cgaggtggta gtggagccac ggcgtcgtcg cgaggaagag cgcgctcgcg acgagctcgc
     4141 catcgaagcg cccgtccacg acgagcagcc gctcccgcag cccggcggcg agcgcgtccc
     4201 agtacgcggc ggagaagaag tagaacgcgt cggcgtcgcg gcggcgcatc gtgtcctcgt
     4261 agagcgcgac gaagtcgccg agctcctccg gcgctggccg gacgaccgtc tcgagcccgg
     4321 cctcgacccc gcggcggatc tcccgccggt gcttcgcgtg catctccccg agcaggtccg
     4381 cgtccgggcg aagcggccag ctcgccgtgt tcgcgagccg ctcgaggcgc gcgcgcggcg
     4441 cggcgtagcg ccagttctcg tggagcgggt ggaagcggac gaacgtcgtc acgacgccct
     4501 cggccgcgca ccactcctcg tagagctcgt ggaagcgcgc gacgggcggg tcggcgccga
     4561 gcgcgagcgg cccgccgtag ccgtagggcg tgacgacgtc gcgggccccg tggcccgccg
     4621 gcacctcgcg cacgatgcac gcgaagacga cgtggccgcc ggcgccctcg aggtggagga
     4681 gcgtcgccgc gcccggctcg acgaggcgtg cggcgtcgaa gaagccgcgc cggaagtagg
     4741 cgtcccggca tccgagcccg tcgaggagcc cgtcccacgc ggcgggctcc acctcgcgga
     4801 ggcgcccggc gccggccgcg cccacgtcag acgaccttcg cggccaggta gcggacgctc
     4861 gccgcggccg ggagcgggtc gtcgagcgcg aagaccgcgc gcaccttcgg ccgccgcgcg
     4921 tacggcgcga ggaagccgag cggcccgagc tcccggcgca gcgccatctg cgcgctcacc
     4981 caggcgtcct tgccgaagaa gatccagacg agctcgtcgg tgaacgtccg gggcggcggc
     5041 agcggccgcc ccgcggcgtc gtcgtaggcg atccgcgcga cgtcgaagag gtccgtcatc
     5101 gcgatcccgc cccacatggg cacgcgcgtg ttcacctcga ggagcttgaa cgagtcgtcg
     5161 cgcgcgtcgt gggcgaactc gacgtggccg aagccgcggt agccgacgct ctcgagcagc
     5221 cggagccccg cttcgagcac cctcggctcg tcgcgcgtct cgaagacggc gctcgtcccg
     5281 aagcggaccg gcccctgccg gatcttccgc ccgacgacgt tcgcgcgcgc gacgccgctc
     5341 cggtcgatgt acgtgaagag ggagaagatc ttctcgtgcg agtcggggac gtgctcctgc
     5401 acgaccatgt cgaagccacg ctcgcgcgcc cagcgccacg cgtcgaccgc ctcgtccacc
     5461 gtcgccgccg ggagcacctt ccgcccgaac gccatcgcga agtgctggcc ctcgaccggc
     5521 ttcacgacga gcgggagcac gaggtcggcc gagcggatgt ccccctcgtg ctcgggagcg
     5581 atcgtctgcg gcgcgggcac gcccgcgcgc tcggcctcgg ccaggacgag ctccttccgc
     5641 cgcagccggt gcacgacgtc cgggtcggac ggcaggggga tcgtcccgag cgccgtgatc
     5701 tcgtcccagt tgcggaggac gagctcgacg tggtcgtccc gctccgggac gatgaagagc
     5761 ggcccggcgg cggcgagctt gcgcaggagc tcgagcacgc gctcgtcccg gcggcgctcg
     5821 tccgcgtcca gcacctcggc ccggcgcgtg aggtagcgcg agcggagacc gaactcgtgc
     5881 tcgtggatca cgatcccgct cacgtcgacg ccggcgcgac cgaggctccg cgcaagcgag
     5941 aggccggccc agaggagccc gaggacggcg acgcgcggcc cgctcacggc tcccgctcct
     6001 cggcggcgcc gcgctgcgcc cgctccacga tcagcgggtc gacgaccggc acgggctccg
     6061 gccggaagag ctgcccgacc gtgcggagga ggatcctcgc gtcgagccag agggaccagt
     6121 gctcgagata ccaggcgtcg taggcgaagc ggtcggccca cgtctggtcc tcccg
//
LOCUS       DASTFC010000257         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_418217,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000257 DASTFC010000000
VERSION     DASTFC010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418217"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFO88_07700"
     CDS             <1..509
                     /locus_tag="VFO88_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET9545446.1"
                     /translation="AVPAPPGVHTVETPTAADLAREALARGPDADVVLMAAAVADYRP
                     ADPSAAKRPKDAEPWAVTLEPTQDVLAELGRGARENGRESVLVGFAADHGERGLERAR
                     EKLAAKNGTLFVFNDVSRDDIGFDSELNEVTVVSESGEHVIGKRDKRAVAAAILDEVA
                     TQLGGGQG"
     gene            510..1484
                     /locus_tag="VFO88_07705"
     CDS             510..1484
                     /locus_tag="VFO88_07705"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9545447.1"
                     /translation="MEEPLARVEEPLGTGRSSELARRIVENLRRVMHAPDETLDFAVL
                     CLFSEGHLIIEDLPGVGKTTLAKSLARSVDLSFSRIQFTPDLLPSDVTGVNVYNQATH
                     EFDFRPGPVFANVLLVDEINRTSPKTQAALLEAMQERQVTVDGATYGLAPPFIVMATQ
                     NPIEYEGTYPLPEAQLDRFTMRISIGYPPLAAEAQMLVEQTTETPLENLEPVASAQEV
                     LAAIEDSRRIFVEESVRRYVVALVRSTREDPRLFLGASPRAGINLLRVGKARALTADR
                     EYVVPDDVKAVAPAVLGHRLILAPEARSAGLDPGDVVRDALERTPVPL"
     gene            1496..2758
                     /locus_tag="VFO88_07710"
     CDS             1496..2758
                     /locus_tag="VFO88_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9545448.1"
                     /translation="MLTDRGRAALALGGLTYLGAWSFGGRMLYPIALGLVLAVAGAAL
                     WVRLVNRPMRLRRSVWEGEHLAGDDVHLGVEIEVHGTMLPAAPVLRERLARLGEHEAE
                     LRRVAGGHGLAGSYTLSRVPRGRYAIESSAVVLEDPFGLARADVEVTRPDALLVYPRL
                     ADLDEVFTDAGGQTPGGRQLLLRRTTGFELHSVREYEQGESLRRVHWPSTARRGELMV
                     KELEDAPRDESAVFLDACAGAVVGEGPDSTFELAVRAAGSLVRLHAKRGRRTSLVVST
                     PGRLYQRVGSLDGEWHRALELLAAVEPTGHLAASAFLADETGAAARALDLTVVTASLD
                     GRLVDVLLQRALARHGASLVYVDPASFVPDGRRLPEVNGQLVRLDRAGVPAIVLRRGD
                     DLAARLAGSLEARQGSAAVAAAAGGVRG"
     gene            2751..>3733
                     /locus_tag="VFO88_07715"
     CDS             2751..>3733
                     /locus_tag="VFO88_07715"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HET9545449.1"
                     /translation="MGRALLGTALAAAVVLPAWLSLHADPRPWNAAVGVALLALVPVA
                     LAGVARRLVVAGVAAAAAFVALGIAFERPLGDALPGGERGFFGPVLSSFRQGVLDFFD
                     TRVPFSALEFPTMRGTVLVAIFAFVLAIGLALVARRTLVAIALVVVGTGWPTTMSFAV
                     DDSNRPLLLGAAVLAVVLALLVLARASTRRLATAGVVGLALVAAAVAGSTNDAVAKEG
                     FADWASWDFYDRPDDPVDVAYVWDANYDGIEFPEKRTTVFTVETEGVRRSLYWRATSL
                     DEFTGRVWRESLETAAFFNPGEPIDVVPIDPLLPDEARDSENWVRQDVRIAA"
BASE COUNT          443 a         1398 c         1370 g          522 t
ORIGIN      
        1 tcgccgtccc ggcgccgccc ggtgtccaca cggtcgagac gcccaccgcc gccgacctgg
       61 cgcgcgaggc gctcgcgcgc ggcccggacg cggacgtcgt cctcatggcc gccgccgtcg
      121 ccgactaccg acccgccgat ccgagcgccg cgaagcgccc gaaggacgcc gagccgtggg
      181 cggtgacgct cgagccgacg caggacgtcc tcgccgagct cggccgcggg gcgcgtgaga
      241 atggacgcga aagcgttctg gtcggcttcg ccgccgacca cggcgagcga ggtctcgaac
      301 gcgcccgcga gaagctcgcg gcgaagaacg gaaccctttt cgtcttcaac gacgtatccc
      361 gtgacgacat cggattcgac agcgaactga acgaggtgac cgtcgtctca gagagcggtg
      421 agcacgtgat cggcaagcgc gacaagcggg ctgtggcggc ggctatcctc gacgaggtcg
      481 cgacccagct cggaggaggc caaggctagg tggaagagcc gctcgcacgc gtcgaggaac
      541 cgctcgggac cggacgctcg tccgagctcg cgcggcggat cgtcgagaac ctccggcggg
      601 tcatgcacgc gcccgacgag accctcgact tcgccgtcct ctgcctcttc tccgaggggc
      661 acctgatcat cgaggacctc cccggcgtcg ggaagacgac gctcgcgaag tcgctcgcgc
      721 gctcggtcga cctgtccttc tcgcgcatcc agttcacgcc ggaccttctc ccgtcggacg
      781 tgacgggcgt gaacgtctac aaccaggcga cgcacgagtt cgacttccgg cccggcccgg
      841 tgttcgcgaa cgtcctgctc gtggacgaga tcaaccgcac ctcgccgaag acccaggccg
      901 ccctgctcga ggcgatgcag gagcgccagg tcaccgtcga cggggcgacg tacgggctcg
      961 cgccgccgtt catcgtgatg gcgacccaga acccgatcga gtacgagggg acgtatccgc
     1021 ttcccgaggc gcagctcgac cggttcacga tgcggatctc gatcggctac ccgccgctcg
     1081 ccgccgaggc gcagatgctc gtcgagcaga cgacggagac gccgctcgag aacctcgagc
     1141 cggtcgcgtc cgcccaggag gtgctggccg cgatcgagga ctcgcggcgg atcttcgtgg
     1201 aggagagcgt gcgccgctac gtcgtcgcgc tcgtccgttc gacgcgcgag gacccccggc
     1261 tcttcctcgg cgcgagcccg cgggcgggga tcaacctcct gcgggtgggg aaggcgcggg
     1321 cgctcaccgc cgaccgcgag tacgtcgtgc cggacgacgt gaaggccgtc gccccggccg
     1381 tgctcggcca ccggctcatc ctcgcgccgg aggcgcgctc cgccggcctc gacccgggag
     1441 acgtcgtccg cgacgcgctc gagcggacgc ccgtcccgct ctgaaggagg cagtcgtgct
     1501 gaccgatcgc ggccgtgcgg cactcgcgct cggcggcctc acgtacctcg gcgcctggag
     1561 cttcggcggg cgcatgctct atccgatcgc gctcggcctc gtcctcgcgg tggccggcgc
     1621 ggcgctctgg gtgcggctcg tgaaccggcc gatgcggctg cggcgctccg tctgggaggg
     1681 cgagcacctc gccggcgacg acgtccatct cggggtggag atcgaggtgc acgggacgat
     1741 gcttcccgcc gcgccggtgc tccgggagcg gctcgcccgt ctcggcgagc acgaggcgga
     1801 gctccgccgc gtcgcgggcg ggcacgggct cgccggctcg tacacgctct cgcgcgtgcc
     1861 gcgcgggcgc tacgcgatcg agtcctccgc cgtcgtcctc gaggatccgt tcggcctcgc
     1921 gcgcgcggac gtcgaggtca cccggccgga cgccctgctc gtgtacccgc gcctcgccga
     1981 cctcgacgag gtgttcaccg atgcgggcgg ccagacgccg ggcgggcgcc agctcctcct
     2041 gcgccgcacg accggcttcg agctgcacag cgtccgcgag tacgagcagg gcgagtcgct
     2101 gcgccgcgtc cactggccct cgaccgcgcg ccgcggcgag ctcatggtga aggagctcga
     2161 ggacgcgccg cgcgacgaga gcgccgtctt tctcgacgcg tgcgcggggg cggtcgtggg
     2221 ggaggggccg gactcgacgt tcgagctcgc cgtgcgcgcc gccgggtcgc tcgtgcgcct
     2281 gcacgcgaag cgggggcgca ggacgtcgct cgtcgtctcg acgcccggtc ggctctacca
     2341 gcgcgtcggc tccctcgacg gggagtggca ccgggcgctc gagctgctcg cggccgtcga
     2401 gccgacggga cacctcgccg cgtccgcgtt cctcgccgac gagacgggcg cggcggcgcg
     2461 cgcgctcgac ctgacggtcg tgacggcgtc gctcgacggg cggctcgtgg acgtgctgct
     2521 ccagcgcgcg ctcgcgcgcc acggcgcctc gctcgtctac gtcgacccgg cgagcttcgt
     2581 gcccgacggg cgccggctcc ccgaggtgaa cggccagctc gtccgcctcg accgcgccgg
     2641 cgtgccggcg atcgtcctgc gccgcggcga cgacctggcc gcccggctcg ccggctcgct
     2701 cgaggcgcgg cagggaagcg cggccgtcgc ggccgccgcc ggaggcgtgc gtgggtaggg
     2761 cgctcctcgg gacggccctc gcggccgcgg tcgtcctccc ggcctggctc agcctccacg
     2821 ccgacccgcg gccgtggaac gccgcggtgg gcgtcgcgct cctcgcgctc gtcccggtgg
     2881 cgctcgccgg ggtcgcgcgc aggctcgtcg tcgcgggcgt ggccgcggcg gccgcgttcg
     2941 tcgcgctcgg gatcgcgttc gagcgcccgc tgggggacgc cctgcccggc ggcgagcgcg
     3001 gcttcttcgg cccggtgctc tcgtccttcc ggcagggcgt gctcgacttc ttcgacacgc
     3061 gcgtgccgtt ctcggcgctc gagttcccga cgatgcgagg gacggtgctc gtggcgatct
     3121 tcgcgttcgt cctcgcgatc ggcctcgcgc tcgtcgcccg gcggacgctc gtcgcgatcg
     3181 cgctcgtcgt ggtcgggacg ggctggccga cgacgatgag cttcgccgtc gacgactcga
     3241 accgcccgct cctcctcggc gcggccgtcc tcgcggtcgt gctggcgctg ctcgtcctcg
     3301 cccgggcgag cacgcgccgc ctcgcgacgg ccggcgtcgt cggcctcgcg ctcgtcgcgg
     3361 cggcggtcgc cggctccacg aacgacgccg tcgcgaagga gggcttcgcc gactgggcga
     3421 gctgggattt ctacgaccgg cccgacgacc cggtcgacgt cgcctacgtg tgggacgcga
     3481 actacgacgg gatcgagttc ccggagaagc gcacgaccgt cttcacggtc gagaccgagg
     3541 gcgtgcgacg gtcgctctac tggcgcgcca cgtcgctcga cgagttcacc ggccgcgtgt
     3601 ggcgcgagtc gctcgagacg gccgcgttct tcaacccggg cgagccgatc gacgtcgtgc
     3661 ccatcgaccc gctcctgccg gacgaggcgc gcgactccga gaactgggtg cgccaggacg
     3721 tgcgcatcgc cgc
//
LOCUS       DASTFC010000258         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1242150,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000258 DASTFC010000000
VERSION     DASTFC010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242150"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..776
                     /locus_tag="VFO88_07720"
     CDS             204..776
                     /locus_tag="VFO88_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9545450.1"
                     /translation="MAHVTGIILGAGLSRRLGRPKQTLPLGDTTLLGWVLREAERSSL
                     DRVVLVTTDDIATTRAEVVSPDGRDPSCTATSLRSGLAAAGETDAVLLLLGDMPGVDA
                     ALVDAVRAEWEATRPWALATEYDDARGHPLVFSAEAVSALRAAEGRKVAWRLLESEPD
                     RVATVRVARPLPRDVDTWEDYEAVLAELAP"
     gene            complement(765..1586)
                     /locus_tag="VFO88_07725"
     CDS             complement(765..1586)
                     /locus_tag="VFO88_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9545451.1"
                     /translation="MIPARFDYELAESVDHAIELLGSGGDAKLLAGGHSLLPLMRLRL
                     ARPSLLVDVGRLDDLRYIREDGGRIAIGALTRHHDVANDPLLREANGLLAHTAAGIGD
                     PQVRHVGTIGGSVAHGDPAADMPAVLVALEAELVVRGAGGERTIAAADCFRGFLETAI
                     EPGEVLTEIRVPRLAGGWSYLKMTRRAQDWATVGVAAVQANGSARVALTNMDATPVRA
                     RGVEEALASGADPAAAAERAAEGTSPPSDHSASGEFRQHLARVLTKRALEEALGS"
     gene            complement(1583..>2281)
                     /locus_tag="VFO88_07730"
     CDS             complement(1583..>2281)
                     /locus_tag="VFO88_07730"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9545452.1"
                     /translation="VALYNAAEKVIAKSRSIVAHKLEVSPDDLEFENGRFTVAGTDKT
                     MTVTDAALHAFTAHDLPDGLEPGLEATFVYDPPNFSWPGGAHIAVVEVDKETGEVDLR
                     RYVAVDEIGNVINPMVVDGQIHGGVTQGIAQALWEEAIYDEDGNLTTGSMVSYLVPSA
                     AETIDYELDRVVAPSPTNPLGVKGVGETGTIASTPAVMNAVVDALAPYGVRDVQMPAK
                     PEHVWRALQEGGAS"
BASE COUNT          271 a          864 c          822 g          324 t
ORIGIN      
        1 gtcgtgcccc ccgtcgagcg cgcgcgtgcc gatgccgcgc gccatgttgc cggtcccgat
       61 gatcgagatc tccatggaat ctcctcctcg cgtgcgcctg ctctgccttt cggcctttcc
      121 gctcggcggg gccgaaaacg cgcgagcgcg tcgcccggcc gcgttcgagc accgagcgcg
      181 gccgtgccta cacttcgcgg gccgtggcgc acgtcaccgg gatcatcctc ggcgcggggc
      241 tctcgcgccg gctcgggcgg cccaagcaga cgctgccgct cggcgacacg acgctgctcg
      301 gctgggtcct ccgcgaggcg gagagatcct cgctcgaccg cgtcgtcctc gtgacgacgg
      361 acgacatcgc gacgacccgc gccgaggtcg tctcgccgga cgggcgcgac ccgtcctgca
      421 ccgcgacgtc gctccggagc ggcctcgcgg cggccggcga gacggacgcg gtccttctcc
      481 tgctcgggga catgccgggc gtggacgcgg ccctcgtcga cgccgtgcgc gccgagtggg
      541 aggcgacgcg cccgtgggcg ctcgcgaccg agtacgacga cgcgcgcggc cacccgctcg
      601 tgttctcggc cgaggcggtc tcggcgctgc gcgcggccga gggaaggaag gtcgcctggc
      661 ggctcctcga gtcggagccc gaccgcgtgg cgaccgtccg cgtcgcgcgg ccgctccccc
      721 gcgacgtgga cacgtgggag gactacgagg cggtgctcgc cgagctagct ccctagggcc
      781 tcctcgagcg cccgcttcgt gagcacgcgg gcgaggtgct gccggaactc gccgctcgcc
      841 gagtggtcgc tcggcgggct cgtgccctcc gcggcgcgct cggccgccgc ggccggatcg
      901 gcgccggagg caagcgcctc ctccaccccc cgagcgcgca cgggcgtcgc gtccatgttc
      961 gtgagcgcga cccgcgccga gccgttcgcc tgcaccgccg cgacgccgac cgtggcccag
     1021 tcctgggcgc ggcgcgtcat cttcaggtac gaccagccgc ccgcaagcct cgggacgcgg
     1081 atctccgtca ggacctcgcc gggctcgatc gccgtctcga ggaacccgcg gaagcagtcg
     1141 gccgccgcga tcgtccgctc tccgcccgcg ccgcggacga cgagctcggc ctcgagcgcg
     1201 acgagcacgg ccggcatgtc cgcggccggg tcgccgtgcg cgaccgaccc gccgatcgtc
     1261 cccacatggc gcacctgcgg gtcgccgatc ccggccgcag tgtgagcgag cagcccgttc
     1321 gcctcccgga ggagcgggtc gttcgccacg tcgtggtggc gcgtgagcgc gccgatcgcg
     1381 atccgcccgc cgtcctcccg gatgtagcgg aggtcgtcca ggcgcccgac gtccacgagg
     1441 agcgacggcc gggcgagccg gagccgcatg agcgggagga gcgaatggcc gcccgcgagg
     1501 agcttcgcgt ccccgccgga gccgagcagc tcgatcgcgt ggtcgacgga ctccgcgagc
     1561 tcgtagtcga agcgggccgg gatcacgacg cacctccttc ctgcagcgca cgccagacgt
     1621 gctcgggctt cgccggcatc tgcacgtcgc gcaccccgta cggcgcgagc gcgtccacga
     1681 cggcgttcat gacagcgggc gtcgaggcga tcgtccccgt ctcgccgacg cccttcacgc
     1741 cgagcgggtt cgtcgggctc ggcgcgacga cccggtcgag ctcgtagtcg atcgtctcgg
     1801 cggcgctcgg gacgaggtag ctcaccatcg agcccgtcgt gaggttcccg tcctcgtcgt
     1861 agatcgcctc ctcccagagc gcctgggcga tcccctgggt cacgccgccg tggatctggc
     1921 cgtccacgac catcgggttg atcacgttgc cgatctcgtc gaccgcgacg tagcggcgga
     1981 gatccacctc gcccgtctcc ttgtcgacct cgacgaccgc gatgtgcgcg ccgcccggcc
     2041 agctgaagtt cggcgggtcg tagacgaagg tcgcctcgag cccgggctcg agcccgtcgg
     2101 ggaggtcgtg cgccgtgaac gcgtggagcg cggcgtcggt gaccgtcatc gtcttgtccg
     2161 tcccggccac ggtgaagcgg ccgttctcga actcgaggtc gtcgggcgag acctcgagct
     2221 tgtgcgcgac gatcgagcgc gacttggcga tcaccttctc ggccgcgttg tagagggcga
     2281 c
//
LOCUS       DASTFC010000259         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_187582,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000259 DASTFC010000000
VERSION     DASTFC010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187582"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFO88_07735"
     CDS             <1..705
                     /locus_tag="VFO88_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9545453.1"
                     /translation="RDGADLHVHREHTDLFYVLEGELVLRLGVEDERVPAGPGTLVRV
                     PPLVVHGFRSGGPGVAYLNLHTPGLEFADYMRAMRDGRPFAYDQHDPPADGGRPTSEA
                     AIGGEVVADRPGLRVELLADVDAIGIAEVRSVPGAAAPPAHVHDRHAESFYVLEGELL
                     LTVGGRELRAGPGTWALVPEGVPHTFAVAGEGPARFLDLHTPSCGFGEFTRRLHAARS
                     DEELAEARASFDQRPA"
     gene            739..1092
                     /locus_tag="VFO88_07740"
     CDS             739..1092
                     /locus_tag="VFO88_07740"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9545454.1"
                     /translation="MARAATTTDAFNAVAEPRRREILDLLAGGERPVNDLVHALGLAQ
                     PQVSKHLRVLRDVGAVDVRSEGRQRLYRLNGRALKPIHDWVSEYERTWSERFDELDVV
                     LEELKEKEARNAGDE"
     gene            1076..1579
                     /locus_tag="VFO88_07745"
     CDS             1076..1579
                     /locus_tag="VFO88_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9545455.1"
                     /translation="MQATSSGTASVTLPADDQILITREFDAPRRLVWEAWTTPELVKR
                     WWNARRGEVTVAEIDLRVDGRWRYVMIADDGTEVAFHGEFREIVPTERIVSTEVFEGL
                     PEGMDPEEHASLNTATFTEADGRTTVAILVQYVGKADRDAVIASGMEAGLQDALDLLE
                     EVAASLR"
     gene            1619..>2265
                     /locus_tag="VFO88_07750"
     CDS             1619..>2265
                     /locus_tag="VFO88_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9545456.1"
                     /translation="MAQPAPWKLLTALWTVYLVWGSTYLAIKVSVETLPPFVSSGLRF
                     LLAGALLAVILAATGRSVRVTRRELGSAAIVGIALLGTGVGVVAQAETRIDSSVAAMI
                     AGTVPLQVILLRTLAREKVARATRLSVLAGLAGLGLIVLPGGSGASSAVGLALMVGAT
                     VSWSLGSFFGQRMPVPRDSFVATTWEMLSAGAFLVVLGAVVGELGTVDAGAFSLES"
BASE COUNT          286 a          821 c          839 g          319 t
ORIGIN      
        1 cgggacggcg ccgacctgca cgtccaccgg gagcacaccg acctcttcta cgtcctcgag
       61 ggcgagctcg tcctgcggct cggggtcgag gacgagcgcg tgccggccgg gcccggcacg
      121 ctcgtgcgcg tgccgccgct cgtcgtgcac gggttccgga gcggcgggcc cggcgtcgcc
      181 tacctcaacc tccatacgcc cggcctcgag ttcgccgact acatgcgcgc catgcgggac
      241 ggccggccgt tcgcgtacga ccagcacgac ccgcccgcgg acggcgggcg gccgacgagc
      301 gaggcggcga tcggcggcga ggtcgtcgcc gaccggccgg gtctccgcgt cgagctcctc
      361 gcggacgtcg acgcgatcgg gatcgccgag gtgcgcagcg tcccgggcgc ggccgcgccg
      421 ccggcgcatg tccacgaccg gcatgcggag tccttctacg tgctcgaggg cgagctcctc
      481 ctcaccgtcg gcggacgcga gctgcgcgcc gggcccggca cgtgggcgct ggtgcccgag
      541 ggcgtcccgc acacgttcgc ggtcgcgggc gaggggcccg cgcgctttct cgacctccac
      601 acgccgagct gcggcttcgg cgagttcacg cgcaggcttc acgcggcgcg ctcggacgag
      661 gagctggccg aggcgcgcgc ctcgttcgac cagcgccccg cttgacatat tcctatatcg
      721 gtatgtattg tgccggccat ggcgagggcg gcgacgacga cggacgcgtt caacgcggtg
      781 gccgagccga ggcggcggga gatcctcgat ctcctcgccg gcggcgagcg ccccgtgaac
      841 gacctcgtcc acgcgctcgg gctcgcgcag ccgcaggtct cgaagcacct gcgcgtcctg
      901 cgcgacgtcg gcgcggtcga cgtccgcagc gagggccggc agcggctcta ccggctgaac
      961 ggccgggcgc tcaagccgat ccacgactgg gtcagcgagt acgagcgcac gtggtccgag
     1021 cggttcgacg aactcgacgt cgttctcgaa gagctcaagg agaaggaggc acggaatgca
     1081 ggcgacgagt agcggcacgg cgagcgtcac gctcccggcg gacgaccaga tcctcatcac
     1141 gcgcgagttc gacgcgccga ggcggctcgt ctgggaggcc tggacgacgc ccgagctggt
     1201 caagcgctgg tggaacgccc gccgcggcga ggtgacggtc gccgagatcg acctccgcgt
     1261 cgacgggcgg tggcgctacg tgatgatcgc ggacgacggg acggaggtcg cgttccacgg
     1321 cgagttccgc gagatcgtcc cgaccgagcg gatcgtctcg accgaggtct tcgagggcct
     1381 cccggagggg atggacccgg aggagcacgc gtccctcaac acagccacgt tcacggaggc
     1441 ggacgggcgg acgacggtcg cgatcctcgt ccagtacgtc ggcaaggcgg accgcgacgc
     1501 ggtgatcgcg tctggcatgg aggcgggtct ccaggacgcg ctcgacctgc tcgaggaggt
     1561 cgcggcctcc ctgcgctgag gccggaatcc ggggcgggcc gggctcgttc ggacctgcat
     1621 ggcccagccc gccccgtgga agctcctcac cgcgctctgg accgtctacc tcgtctgggg
     1681 ctcgacgtat ctcgcgatca aggtctccgt cgagaccctg ccgccgttcg tctcgagcgg
     1741 gctccgcttc ctgctcgcag gcgcgctgct cgccgtgatc ctcgccgcca ccgggcgcag
     1801 cgtccgcgtg acgcggcgcg agctcggctc ggcggcgatc gtcgggatcg cgctcctcgg
     1861 gaccggcgtc ggcgtcgtcg cccaggcgga gacgcgaatc gactcgagcg tcgcggcgat
     1921 gatcgcgggc accgtcccgc tccaggtcat cctcctgcgg acgctcgcgc gcgagaaggt
     1981 cgcccgcgcg acgcggctct ccgtcctcgc cgggctcgcc gggctcgggc tcatcgtcct
     2041 ccccggaggc agcggcgcct cgagcgccgt cgggctcgcg ctcatggtcg gcgcgaccgt
     2101 gtcctggtcg ctcgggtcgt tcttcggcca gcggatgccg gtgccgcgcg acagcttcgt
     2161 cgcgacgacg tgggagatgc tgagcgccgg agccttcctc gtcgtcctcg gcgccgtcgt
     2221 cggcgagctc gggacggtcg acgcgggcgc cttcagcctc gagtc
//
LOCUS       DASTFC010000260         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_648944,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000260 DASTFC010000000
VERSION     DASTFC010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648944"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1053)
                     /locus_tag="VFO88_07755"
     CDS             complement(82..1053)
                     /locus_tag="VFO88_07755"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HET9545457.1"
                     /translation="MSALVSAVDYHTAGEPFRIVTGGVEALRGETILAKRRDAAERLD
                     HVRRLLVNEPRGHADMYGCHVVEPEPGGDLGVVFFHNAGYSTACGHGTIALATAALEL
                     GMVPAREPVTELLLDVPSGRLPVRIEVEGGRPGRVSFVNVPSYVEAEGLAVEGVEADV
                     AFGGAFYASVRSPVPVEPASVPELIGLGRRVKRALEAEREWAHPLEPELRDIYGVIFW
                     QEEDAPFTQRNVTIFADGEVDRSPCGSGTSARLALLDAHGELPRGETLRHLSVIGSEF
                     GARVVGDAEVAGRQAVLTEIAGRAHLTGYHQFVLAEEDELGTGFLLR"
     gene            complement(1050..>2084)
                     /locus_tag="VFO88_07760"
     CDS             complement(1050..>2084)
                     /locus_tag="VFO88_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9545458.1"
                     /translation="AFNVNAMLWRTGPAATELEELACDWLRQLLGLAEGWHGHIEDTA
                     STSTLAALAAARELRPGGVVLCSEHAHSSVDKAAKLLGIGVRKVAVDDEFRLRPDALA
                     EALEADEAAAVVATVGTTSTTSVDPVPAVADLCAEHGAWLHVDAAYAGSAMVCEELRW
                     AFEGVERADSLVVNPHKWLFTPVDCSVLFTRRPEALRDAFSLLPEYLRTNVEDVTNLM
                     DYGPALGRRFRALKLWTVVRCYGREGLQALHREHLRLARLFASWIEAEPGWEVAAPVP
                     FSVVCFRREGTDEENEALMERVNASGEVYLSHTRLGDRLVLRLAVGNARTTEEDVALA
                     WEVLRREAAA"
BASE COUNT          287 a          785 c          764 g          248 t
ORIGIN      
        1 cgagcgctgc gcgggcacgc acttcgaccc gcgcgtcgtc gaggcgctcg tcgcgcacgt
       61 gcgccgcgtg cccgcggccg cctagcggag cagaaacccg gtcccgagct cgtcctcctc
      121 ggcgaggacg aactggtggt agccggtcag gtgcgcgcgg ccggcgatct ccgtcaggac
      181 cgcctggcgg cctgccacct cggcgtcgcc gacgacacgg gccccgaact cgctcccgat
      241 cacgctcagg tggcggagcg tctcgccgcg aggcagctcg ccatgcgcgt cgagcagcgc
      301 gagccgcgcg gaggtcccgc tcccgcacgg ggagcggtcg acctcgccgt cggcgaagat
      361 cgtcacgttc cgctgcgtga acggcgcgtc ctcctcctgc cagaagatga cgccgtagat
      421 gtcgcggagc tcgggctcga gcgggtgcgc ccactcgcgc tcggcctcga gcgcccgctt
      481 cacgcggcgg ccgagcccga tcagctccgg aacggacgcc ggctcgaccg gcaccggcga
      541 gcggacggac gcgtagaacg cgccgccgaa cgcgacgtcc gcctcgacgc cctcgacggc
      601 gagcccctcg gcctcgacgt aggagggcac gttcacgaag gacacgcgac ccgggcgccc
      661 gccctcgacc tcgatgcgga cgggcaggcg gccggacggc acgtcgagca ggagctccgt
      721 caccggctcg cgcgcgggaa ccatcccgag ctcgagcgcc gcggtcgcga gcgcgatcgt
      781 cccgtgcccg cacgcggtcg agtagccggc gttgtggaag aagacgacgc cgaggtcgcc
      841 gcccggctcc ggctcgacga cgtgacagcc gtacatgtcc gcgtgcccgc gcggctcgtt
      901 gacgaggagc cggcgcacgt ggtcgagccg ctcggccgcg tcgcggcgct tggcgaggat
      961 ggtctcgccg cgcagcgcct cgacgccgcc ggtcacgatc cggaacggct cgcccgcggt
     1021 gtggtagtcg accgccgaga cgagcgcgct cacgccgccg cctcccgccg gagcacctcc
     1081 caggcgagcg cgacgtcctc ctccgtcgtg cgggcgttcc cgaccgcgag gcgcaggacg
     1141 aggcggtcgc cgagcctcgt gtgcgagagg tagacctcac cgcttgcgtt cacgcgctcc
     1201 atgagcgcct cgttctcctc gtccgtcccc tcgcgccgga agcagacgac cgagaacggc
     1261 accggcgcgg cgacctccca gcccggctcc gcctcgatcc aggacgcgaa gagccgcgcg
     1321 aggcgcaggt gctcgcggtg gagcgcctgc aggccctcgc gcccgtagca gcggacgacc
     1381 gtccagagct tgagcgcgcg gaagcggcga ccgagcgcgg gcccgtagtc catgaggttc
     1441 gtcacgtcct cgacgttcgt gcggaggtac tcgggaagga ggctgaacgc gtcgcggagc
     1501 gcctccggcc ggcgggtgaa gagcaccgag cagtcgaccg gcgtgaagag ccacttgtgc
     1561 gggttgacga cgagcgagtc ggcgcgctcg acgccctcga aggcccagcg gagctcctcg
     1621 cagaccatgg ccgagcccgc gtacgccgcg tcgacgtgga gccaggcgcc gtgctccgcg
     1681 cagaggtcgg cgaccgccgg gaccgggtcg accgacgtgg tcgaggtcgt cccgaccgtc
     1741 gcgacgaccg cggcggcctc gtcggcctcg agcgcctcgg cgagcgcgtc cgggcgaagc
     1801 cggaactcgt cgtcgaccgc gaccttccgc acgccgatcc cgagaagctt cgcggccttg
     1861 tcgacggacg agtgcgcgtg ctcggagcag aggacgaccc cgccgggccg aagctcgcgc
     1921 gcggcggcga gcgccgcgag cgtcgaggtc gacgccgtgt cctcgatgtg cccgtgccag
     1981 ccctccgcga ggccgagcag ctgccggagc cagtcgcacg cgagctcctc gagctcggtc
     2041 gcggccgggc cggtgcgcca cagcatcgcg ttcacgttga aggc
//
LOCUS       DASTFC010000261         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_121458,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000261 DASTFC010000000
VERSION     DASTFC010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121458"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFO88_07765"
     CDS             complement(<1..342)
                     /locus_tag="VFO88_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET9545459.1"
                     /translation="MIPLALDEVAELAPGRLDRSPDAPAEVTGLTIDSRRVAPGDLFV
                     AVGRGAEFADDALAAGAAAALVPEDAFTALAALGRAVRERSDAHVVAITGSIAKTSTK
                     DILAALCRPHRR"
     gene            complement(339..1784)
                     /locus_tag="VFO88_07770"
     CDS             complement(339..1784)
                     /locus_tag="VFO88_07770"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET9545460.1"
                     /translation="MRPLRTLNAAAVDVADLAYDTRAVAPGALYACLPGFRADGHDFA
                     PDAVARGAVALIVERELAGLPVPQLVVDDARVALGLAADAFFGSPTRELRIAGVTGTN
                     GKTTTAFLTYAILAAAGRRPGLVGTIEARVGGERRAVTHTTPASVDLQRTFREMLDAG
                     DRTCALEASSHGSELKRLVGVRFAALAFTNLSTEHLDLHGTLDAYFDAKRRLFVEPDV
                     DGHRPPAAVNTGDEHGRRLADELRALGAAPLLTFALDGPADLVPRELEVTPAGSRFRL
                     GDLELETRLRGRFNVENVLAAVALARLLDVPDEAIARGVAHLTGVPGRFEAVDEGQPF
                     TVLVDYAHTPGALENVLAEARALATGRLAVVFGCGGDRDRGKRPLMGEVATRLADDVT
                     VTSDNPRSEEPAAIADEILEGVHGEPEVELDRAAAIERALARAAPGDVIVIAGKGHEP
                     GQEAAGVITPFDDREVARDALRRLRAGAGAA"
     gene            complement(2012..2641)
                     /locus_tag="VFO88_07775"
     CDS             complement(2012..2641)
                     /locus_tag="VFO88_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545461.1"
                     /translation="MLAELDGLAAEVERIRAEAAATVDRLERLPGEQAAARAEREEAE
                     AAVGERRAAHRAAAEELAQAEGGRDDTRTAEARRAELRARDLLRSAERRAERAGADEE
                     RLEAERDALARGAGELDRRARALADELARRPGLSDAAPPPEGAGPAGPAAWATETRAA
                     LFVARGRLASEREAVIRQANELGALVLGEPLTAQSAALVARRVEEHGSR"
     gene            complement(2724..>3500)
                     /locus_tag="VFO88_07780"
     CDS             complement(2724..>3500)
                     /locus_tag="VFO88_07780"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HET9545462.1"
                     /translation="ASSRARNKIRQWLSRETREETETRGREALEEALKQHKLPYKKLA
                     GSPLLAELIREMGFKKAEDFYLALGAGKLAAGQVANKVLNRLKTAEVAEEEPVPTKPR
                     AKLAVASQDFGINVDGLEGVLVRLAKCCTPVPGDPIVGYISLGKGITIHRADCPNVKA
                     LMRSPERFTAVEWDGGASQSFRVHIAVTSWDRPRLLEDVARTFAEHGANIVEYGGHVE
                     DQMAKNWYVAEVGDVGSLRALLSALRNIDGVFDAFRVTPS"
BASE COUNT          401 a         1352 c         1310 g          437 t
ORIGIN      
        1 gcggcggtgc gggcggcaga gcgccgcgag gatgtccttc gtcgacgtct tcgcgatcga
       61 gcccgtgatc gcgacgacgt gcgcgtcgct ccgctcccgg acggcgcggc cgagcgcggc
      121 gagggcggtg aacgcatcct cggggacgag cgcggcggcc gcgcctgcgg cgagcgcgtc
      181 gtccgcgaac tccgcgcctc ggccgacggc gacgaagagg tcgcccggcg cgacgcggcg
      241 ggagtcgatc gtgagaccgg tgacctcggc gggcgcgtcc ggcgagcggt cgagccggcc
      301 gggggcgagc tccgcgacct cgtcgagcgc gagcgggatc acgcggcgcc ggccccggcg
      361 cggaggcggc ggagcgcgtc gcgcgccacc tcgcggtcgt cgaacggcgt gatgacgccg
      421 gccgcctcct ggcccggctc gtgccccttg ccggcgatca cgatcacgtc gccgggggcg
      481 gcgcgcgcga gcgcccgctc gatcgccgcg gcgcggtcga gctcgacctc cggctccccg
      541 tgcacgccct cgaggatctc gtccgcgatc gccgccggct cctcgctgcg cgggttgtcc
      601 gacgtgacgg tcacgtcgtc cgcgagccgg gtcgcgacct cgcccatgag cggccgcttg
      661 ccccggtcgc ggtcgccgcc gcagccgaag acgaccgcca gccggccggt cgcgagcgcg
      721 cgtgcctcgg cgaggacgtt ctcgagggcc cccggcgtgt gcgcgtagtc gacgagcacc
      781 gtgaagggct gcccctcgtc gaccgcctcg aagcggcccg ggacgccggt caggtgcgcg
      841 acgcctctcg cgatcgcctc gtccgggacg tcgaggaggc gggcgagcgc gacggcggcg
      901 agcacgttct ccacgttgaa gcgcccccgc aggcgcgtct cgagctcgag gtcgccgagc
      961 cggaagcgag agccggcggg cgtgacctcg agctcccgcg ggacgaggtc ggccggtccg
     1021 tcgagcgcga acgtgaggag cggcgcggcg ccgagcgcgc ggagctcgtc cgcgagccgg
     1081 cgcccgtgtt cgtcgccggt gttcacggcg gcgggcgggc ggtgcccgtc cacgtccggc
     1141 tcgacgaaga ggcgccgctt cgcgtcgaag tacgcgtcga gcgtgccgtg caggtcgagg
     1201 tgctcggtcg agaggttcgt gaacgcgagc gcggcgaagc ggacgccgac gaggcgcttg
     1261 agctccgagc cgtgcgacga ggcctcgagc gcgcacgtgc ggtcgcccgc gtcgagcatc
     1321 tcgcggaacg tgcgctggag gtcgaccgag gccggggtcg tgtgcgtgac cgcgcggcgc
     1381 tcgccgccga cccgcgcctc gatcgtcccg acgaggccgg gccgccggcc cgccgcggcg
     1441 agaatcgcgt acgtgagaaa cgcggtcgtc gtcttcccgt tcgtgcccgt gacgcccgcg
     1501 atccggagct cacgcgtcgg gctgccgaag aacgcgtcgg cggcgagccc gagggcgacg
     1561 cgggcgtcgt ccaccacgag ctgcgggacc ggcaggccgg cgagctcgcg ctcgacgatg
     1621 agcgcgaccg cgccgcgcgc caccgcgtcg ggggcgaagt cgtgcccgtc ggcgcggaag
     1681 ccgggcaggc acgcgtagag cgcccccggc gcgaccgcgc gcgtgtcgta cgcgaggtcg
     1741 gcgacgtcga cggcggcggc gttcagcgtg cggagcggcc gcacggcctc gctgatgcgg
     1801 tcgagctcga tggcgagcga ttctaccgga ggcctccccc ggatcccgca ccaggaccgg
     1861 agcgaaatcc ctgctcacgg cgcactttcg acacgatttc tttgcgattt cggcacacgc
     1921 cgcgcggccg gcgcgggcta cgctgaccct agggacggtc cggacggggg gattagacgc
     1981 gcgcgcgtct agcggtcgca gaacccacag ctcaccgcga gccgtgctcc tccaccctgc
     2041 gcgccacgag cgccgcgctc tgcgccgtga ggggctcccc gagcacgagc gcgccgagct
     2101 cgttcgcctg gcggatcacc gcctcgcgct cggacgcgag ccgcccgcgc gcgacgaaga
     2161 gcgccgcgcg cgtctccgtc gcccacgccg ccggcccggc cggccctgcg ccttccggcg
     2221 gtggggccgc gtcggagagc ccgggccgcc gcgccagctc gtccgcgagc gcacgcgcgc
     2281 gccggtcgag ctcgcccgcg ccgcgcgcga gcgcatcccg ctccgcctcc aggcgctcct
     2341 cgtccgcccc ggcccgctcg gcgcggcgct cggccgagcg gagcagatcg cgggcgcgga
     2401 gctcggcccg ccgcgcctcc gccgtgcgcg tgtcgtcccg gccgccctcc gcctgggcca
     2461 actcctccgc cgcagcgcgg tgcgccgccc ggcgctcccc gacggccgcc tcggcctcct
     2521 cccgctccgc ccgggcggcg gcctgctctc ccggaagccg ctcgagccga tcgacggtcg
     2581 ccgccgcctc ggcgcggatc cgctcgacct ccgccgcgag cccgtcgagc tcggccagca
     2641 cggacgcggc ggcctcgtcc gcccgcgcga gctcctggag gaacgcgacc gtccgctcgt
     2701 cggcgctcag cgggccgcgc ccctcacgac ggggtgacgc ggaaggcgtc gaacaccccg
     2761 tcgatgttgc ggagcgcgct caggagcgcc cgcagcgagc cgacgtcgcc cacctccgcg
     2821 acgtaccagt tcttcgccat ctggtcctcg acgtggccgc cgtactcgac gatattcgcg
     2881 ccgtgctcgg cgaacgtccg cgcgacgtcc tcgagcagcc gcggccggtc ccacgaggtc
     2941 accgcgatgt ggacgcggaa gctctgcgac gcaccgccgt cccactcgac ggcggtgaac
     3001 cgctccggcg agcgcatgag cgccttcacg ttcgggcagt ccgcccggtg gatcgtgatg
     3061 cccttgccga gcgagatgta gccgacgatc gggtcgcccg gcaccggggt gcagcacttt
     3121 gcgagccgga cgaggacgcc ctcgagcccg tcgacgttga tcccgaagtc ctgcgaggcg
     3181 accgcgagct tcgcgcgcgg cttcgtcgga acgggctcct cctcggcgac ctcggcggtc
     3241 ttgagccggt tcagcacctt gttcgccacc tggcccgccg cgagcttccc cgcgccgagc
     3301 gccaggtaga agtcctccgc cttcttgaag cccatctcgc ggatgagctc ggcgagaagc
     3361 ggcgacccgg cgagcttctt gtacgggagc ttgtgctgct tcagcgcctc ctcgagcgcc
     3421 tcgcggccgc gcgtctcggt ctcctcgcgc gtctcgcgcg agagccactg gcggatcttg
     3481 ttccgcgcgc gcgaggaggc
//
LOCUS       DASTFC010000262         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1226038,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000262 DASTFC010000000
VERSION     DASTFC010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226038"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFO88_07785"
     CDS             complement(<1..227)
                     /locus_tag="VFO88_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET9545463.1"
                     /translation="MSAPEDATRRTRLAAERTYLAWWRSALGAFAVALAAGKLVPALT
                     DAEDWPFVLLGTGFGLVGVAFVALALWRHRRV"
     gene            complement(224..1075)
                     /locus_tag="VFO88_07790"
     CDS             complement(224..1075)
                     /locus_tag="VFO88_07790"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET9545464.1"
                     /translation="MRPTPEQIEAYRRDGFLVVEEFLPPDELAPVRERFARAFAHEWE
                     TGLQPDEVNYVPGVTPPELTRQLCNVWKADRVLAATVLSRRVGEFCTALSGHPGLRLA
                     QDNAIWKPPAGKALLAHQDAAYLEFLDPPNMTTCWMALDDTAADTGTIYYVRGSNRWP
                     RAPRGGEFHAPDDWLAHARAVMPESEELDLVPIEVPAGGAAVHDGWVFHGSPPNERAD
                     AERRAIISHLISTETRWSDAGVHPIYSKYRRPGERELDEAFFPILWREDGYRTPWLDE
                     LERAAAA"
     gene            complement(1096..1374)
                     /locus_tag="VFO88_07795"
     CDS             complement(1096..1374)
                     /locus_tag="VFO88_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545465.1"
                     /translation="MTDDGHGATRGPSAPDPYWLRHSHGFRVVSARGRVGIVEDVLYG
                     ADRERPSALAVRGGLFGTRVEIVPVEEVAHVIPRSKLLSLRADQRRAA"
     gene            complement(1397..2764)
                     /locus_tag="VFO88_07800"
     CDS             complement(1397..2764)
                     /locus_tag="VFO88_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545466.1"
                     /translation="MTPYRDVELGAALRGLEVPEHRPEFDALLRERLAQERRRARVAP
                     PRRRVQRLRIAALAAALAVAAAVAGVPLLDRATDLGGGPEPATAAEIQERIRVALAEL
                     ESLSGVLVEDGPQAGDEARWRFTLTTRGDFRITGLTVDEDASYDAEAGLYRSSAVSES
                     LGGEGPRFHSELRGVAPGPPDESPASWRLPRTFGAVVRAFLGEEELRVDEIEHDGRPA
                     WRLALDAVPNAIVPDVSGDRLEIVVDRESGLPLRVLETRNGAFVRSLTLEDVAVDEPP
                     APSTFRLELPAGAEVDRRDVGFRRVALDEVERAVGYAPLVPAWVPEGYELAEVAVARE
                     GSFTGTEAGNPRSLRVVSLSFRRGFDQFLVTTRLADVPNGSGLPVEERWSDPLATGEG
                     FRDEPEPLELTEGALAGARAELLLAPRATPHVWAVTPELVVTVNGDLTRAELLRVTES
                     LRRDG"
     gene            complement(2761..3330)
                     /locus_tag="VFO88_07805"
     CDS             complement(2761..3330)
                     /locus_tag="VFO88_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545467.1"
                     /translation="MSARSATRTREPDAGVLARARDGDEEAFAVLVRHYDPGLRALAF
                     RLLGSRERMDDALQEAYVRAYRALPRFRGDAKLATWLYRIVYNACLDELERARRGGLV
                     LVPDLPDEADPAPEPGEAAARRDELARALAGLPPEQRAAVLLVDAQGFTYRAAGRILG
                     VPEGTVASRLNQARAVLRAALEDEEEALG"
     gene            3664..>4217
                     /locus_tag="VFO88_07810"
     CDS             3664..>4217
                     /locus_tag="VFO88_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545468.1"
                     /translation="MQGPGGVAAGAGSAGLLEREAELEAVEAALATAAQGEGRLVILE
                     GEAGIGKSALLRAARASAEEAGGRVLSARGGELERSFSFGLVLQLFEAPLRAADEAER
                     QQLLAGAAALARPLFEPGGEHPSAGEDLELLHGLYWLTANLAERSPLVLVVDDLHWGD
                     PSSLRFLTYLAPRLESLPVAVLVSL"
BASE COUNT          526 a         1602 c         1571 g          518 t
ORIGIN      
        1 acgcgccggt ggcgccacag ggcgagcgcg acgaacgcga cgccgacgag cccgaagccg
       61 gtgccgagca ggacgaacgg ccagtcctcg gcatccgtga gcgcggggac gagcttcccg
      121 gccgcgagcg cgaccgcaaa cgcgccgagc gcgcttcgcc accacgcgag atacgtccgc
      181 tcggcggcga gccgggtgcg ccgtgtcgcg tcctcggggg cgctcacgcc gccgccgcgc
      241 gctcgagctc gtcgagccag ggcgtgcggt agccgtcctc gcgccagagg atcgggaaga
      301 acgcctcgtc cagctcgcgc tcgcccgggc ggcggtactt cgagtagatc gggtggacgc
      361 cggcatcgct ccagcgcgtc tccgtcgaga tgaggtggct gatgatcgcc cgtcgctcgg
      421 cgtcggcgcg ctcgttcggc gggctgccgt ggaagaccca gccgtcgtgc acggcggccc
      481 cgccggccgg cacctcgatc gggacgaggt cgagctcctc gctctcgggc atgaccgcgc
      541 gcgcgtgcgc gagccagtcg tcgggcgcgt ggaactcgcc gccccgcggc gcccgcggcc
      601 agcggttcga gccgcggacg tagtagatcg tccccgtgtc ggccgccgtg tcgtcgagcg
      661 ccatccagca ggtcgtcatg ttcggcgggt cgaggaactc gaggtacgcc gcgtcctggt
      721 gcgcgagaag cgccttgccg gcgggcggct tccagatcgc gttgtcctgc gcgagccgca
      781 ggcccgggtg gccggagagc gccgtgcaga actcgcccac gcgccgcgac agcacggtcg
      841 ccgcgagcac gcgatccgcc ttccagacgt tgcagagctg gcgggtcagc tcgggcggcg
      901 tgactcccgg gacgtagttg acctcgtccg gctggaggcc cgtctcccat tcgtgcgcga
      961 aggcgcgggc gaagcgctcg cgcacgggcg cgagctcgtc cgggggcagg aactcctcga
     1021 cgacgaggaa cccgtcgcgc cggtaggcct cgatctgctc gggcgtcggc cgcacgcgcc
     1081 caatgatggc agacgtcacg cggcccttcg ctggtccgcg cgaagcgaaa ggagcttcga
     1141 gcgcgggatc acgtgagcga cctcctcgac cgggacgatc tccacgcgcg tgccgaagag
     1201 cccgccgcgc accgcgagcg ccgacggtcg ttcgcggtcg gcgccgtaga ggacgtcctc
     1261 gacgatcccg acccgcccgc gcgcggacac gaccctgaag ccgtgcgaat ggcgcagcca
     1321 gtaggggtcc ggggcgctcg ggccccgcgt cgctccgtgt ccgtcgtccg tcatgcgatc
     1381 cctccctttc tcggacctat ccgtcccttc gcaacgactc ggtcacgcgg agcagttccg
     1441 cgcgcgtgag gtcgccgttc accgtcacga cgagctctgg cgtgaccgcc cacacgtgcg
     1501 gggtcgcccg cggcgcgagc aggagctcgg cccgcgcgcc ggcgagcgcg ccctccgtga
     1561 gctcgagcgg ctccggctcg tcgcggaagc cctcgccggt cgcgagcggg tcgctccacc
     1621 gctcctccac cggcagcccc gacccgttcg gcacgtcggc cagccgcgtc gtcacgagga
     1681 actggtcgaa cccgcgccgg aaggagagcg agacgacccg cagcgagcgc gggttcccgg
     1741 cctcggtccc cgtgaaggag ccctcgcgcg ccaccgcgac ctccgcgagc tcgtatccct
     1801 ccggcaccca ggcgggaacg agcggcgcgt agccgacggc gcgctccacc tcgtcgagcg
     1861 ccacgcggcg gaagccgacg tcgcgccggt cgacctccgc gccggccggg agctcgagcc
     1921 ggaacgtcga gggcgccgga ggctcgtcga ccgcgacgtc ctcgagcgtg agcgagcgca
     1981 cgaaggcgcc gttcctcgtc tcgagcacgc ggagcggcag gccggactcg cggtcgacca
     2041 cgatctcgag ccggtcgccg gagacgtccg ggacgatcgc gttcgggacg gcgtcgagcg
     2101 cgagccgcca ggcgggccgg ccgtcgtgct cgatctcgtc gacgcgcagc tcctcctcgc
     2161 cgaggaacgc ccggacgacc gcgccgaacg tccgcgggag ccgccacgac gccgggctct
     2221 cgtcgggcgg gccgggcgcc acgccgcgca gctcgctgtg gaagcggggg ccctccccgc
     2281 cgagcgactc gctgacggcg ctcgagcggt agagcccagc ctccgcgtcg tacgacgcgt
     2341 cctcgtcgac cgtgaggccg gtgatgcgga agtcgccgcg cgtcgtcagc gtgaagcgcc
     2401 agcgcgcctc gtcgcccgcc tgcggcccgt cctcgacgag gacgccgctc aggctctcga
     2461 gctcggcgag cgcgacgcgg atccgctcct ggatctcggc cgcggtcgcc ggctccgggc
     2521 cgccgcccag atcggtggcg cggtcgagga gcgggacgcc ggcgacggcg gcggcaacgg
     2581 ccagggctgc ggcgagcgcc gcgatgcgaa gccgctggac gcggcgccgg ggcggcgcga
     2641 cgcgggcgcg ccggcgctcc tgcgccaggc gctcgcggag cagcgcgtcg aactccgggc
     2701 ggtgctccgg aacctcgagc ccgcgcagcg cggctcccag ctcgacgtca cggtaagggg
     2761 tcatccgagc gcctcctcct cgtcctcgag ggcggcgcgc aggacggcgc gcgcctggtt
     2821 caaccgcgac gcgaccgtcc cctccggaac cccgaggatc cggcccgccg cgcggtaggt
     2881 gaagccctgt gcgtcgacga gcagcactgc ggcccgctgt tcgggcggta gccccgcgag
     2941 cgcacgggcg agctcgtcgc ggcgcgcggc cgcctcgccc ggctccggcg ccggatccgc
     3001 ctcgtccggg aggtcgggaa cgaggacgag cccgcctcgg cgtgcgcgct cgagctcgtc
     3061 gaggcacgcg ttgtagacga tgcggtagag ccaggtggcg agcttcgcgt cgccgcggaa
     3121 gcgcgggagc gcccggtagg cgcggacata cgcctcctgg agcgcgtcgt ccatccgctc
     3181 ccggctgccg agcagccgga aggcgagcgc gcggagcccc ggatcgtagt ggcggacgag
     3241 cacggcgaac gcctcctcgt cgccgtcccg tgcccgggcg aggacgccgg catccggctc
     3301 acgggtgcgg gtcgcggaac gcgcgctcac actcgtttcg accgctcgga gagccgaaag
     3361 tcttcggcgg gactaggggc gcgaccggct gccccgccgc cccgacgcgc cgccgcacgg
     3421 gcggtcggaa cggacaccgg acgcgcccca gggagggcgc aacggggacg ccgcaaccag
     3481 gaagctgcgt ccccgttccc cacacgccct cgaccccggt cccctcctgg cgtctcgatc
     3541 gacacccgcc tgccgccgaa gcggcggccg cctgccccca agcgatcgat gtcgggacgc
     3601 catggcgaga cggtacgcct ccgttctccg tatggcacta cacccccggg ttagtctttt
     3661 cgcatgcagg gtcccggcgg cgtggccgca ggcgccggta gtgcagggct gctcgagcgg
     3721 gaggcggagc tcgaggcggt cgaggccgcg ctcgcgacgg ccgcgcaggg cgaggggcgg
     3781 ctcgtcatcc tcgagggcga ggcgggaatc ggcaagtccg cgctcctccg cgcggcgcgt
     3841 gcctcggcgg aggaggcggg cgggcgagtc ctctcggcgc gcggcggcga gctcgagcgc
     3901 tcgttctcct tcgggctcgt cctccagctc ttcgaggcgc cgcttcgcgc cgccgacgag
     3961 gccgagcggc agcagctcct cgcgggcgcc gccgcgctcg cgcgcccgct cttcgagccg
     4021 ggcggcgagc atccgtcggc aggcgaggac ctggagctcc tgcacggcct ctactggctg
     4081 acggcgaacc tcgccgagcg ctcgccgctc gtgctcgtcg tggacgacct ccactggggc
     4141 gacccgtcgt cgctccgctt cctgacgtac ctcgcgccgc ggctcgagag cctgccggtc
     4201 gccgtgctcg tctccct
//
LOCUS       DASTFC010000263         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_813884,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000263 DASTFC010000000
VERSION     DASTFC010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813884"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="VFO88_07815"
     CDS             complement(<1..1674)
                     /locus_tag="VFO88_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9545469.1"
                     /translation="MQELFGIPVGTLALVLSALVLGALAVLGVFALRNRVFVRLGVRN
                     VRRRPARSALIVTGLMLGTAIVAAALATGDTMSHTIRQSSLTALGETDEVVSVAGAEA
                     ELMTGEVEAAAVRYFPEGHYREIAAALEGSDLVDGVAPAVIEPVAVQGVESRLSEPRA
                     TLFGSDPAALAEMSPIRDLAGETRSLADLGPGAVFVNEEAAEELAVTDGDKLRVLAGE
                     TVVPMRVEAVVSFRGAGADGPALLVPLGPAQDLVGEPGQIRHVLVSNRGDAMSGAALS
                     DEVIELLEPTLTRLALEAEPAKADALETADLGGASFMSLFTTFGSFSIAAGLLLIFLI
                     FVMLAAERRAELGIARAVGTRRGHLVQTFLYEGVTYALLAAAVGALVGIAVAYVMVLV
                     VASAVAAESDLEIAYTVSPKSVVVAYSVGVVLTFLVVGLSAWRVSRMNIVTAIRNLPE
                     PPAQKGQRRRLLFGGLAIALGALLAMSGYGSDDVTTLGLGVGLAIMGLVPVARALGVS
                     ERVARTTAGFALVAFFLLPTDEWILGQPKQNFSIFIVAGFMIVIGATWIL"
     gene            complement(1678..1929)
                     /locus_tag="VFO88_07820"
     CDS             complement(1678..1929)
                     /locus_tag="VFO88_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545470.1"
                     /translation="MFESRIAPALREQDGFEGVYALSTPEGKALVMTLWRDAETAEAG
                     LASGLYDEQVEQYVAYFRSPPGRELYEVVHAETPAGAAA"
     gene            complement(1895..2695)
                     /locus_tag="VFO88_07825"
     CDS             complement(1895..2695)
                     /locus_tag="VFO88_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9545471.1"
                     /translation="MATEQIIEARGVHKTYDTGKVEVRALRGVDLAVARGEMVAIMGP
                     SGCGKTTLLNCLSGLDAIDAGEIEIEGVPLAGMSDRARTDYRARRMGFVFQFYNLMPV
                     LSSVENVELPLLVARVHAREARRRAVEALEMVGLERWADHLPDELSGGQRQRVTIARA
                     LVNDPAIVWADEPTGDLDSENAAEITDLMRRLNVERGLSFVIVTHDIGVGRKTDRIVR
                     MLDGEVVEQELLEVSNGRAGDVGGDRHGADAARRRGRRVREPHRAGAA"
     gene            complement(2773..3288)
                     /locus_tag="VFO88_07830"
     CDS             complement(2773..3288)
                     /locus_tag="VFO88_07830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET9545472.1"
                     /translation="MRDALVVIDLLQRFDHEDGDALLASLRGRLPGLVDALDRARADG
                     TPVVYVNDLDGRWDSNAPALVRDALDGPGGDALAEVAPRVGDRILLKPRYSIFDHTPL
                     VVLLRELEVERLLLAGAATEMCVVQSAIDARELGFKVSILAGACATTDPELERLAFEY
                     AERVVGARVER"
     gene            complement(3309..3668)
                     /locus_tag="VFO88_07835"
     CDS             complement(3309..3668)
                     /locus_tag="VFO88_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9545473.1"
                     /translation="MTVRAAADLIQPGLLGEAVDTGPALVFVADDDGRYVAVNRRACE
                     VLGYEREELLELHVTDVAVAADAPDLYRALRRRGEAAGTTLLRGKDGSLHPYAYRATE
                     TTTAGMPFWVSVGFVDD"
     gene            complement(3736..>4006)
                     /locus_tag="VFO88_07840"
     CDS             complement(3736..>4006)
                     /locus_tag="VFO88_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545474.1"
                     /translation="TFERYPEIGAVLPAMGYGDEQLAELAATIDAADCDVVVTGTPMN
                     LTRVIDVRHPVRHATYVLADHGEPTLADVLRPFVEAHVPEALVTT"
BASE COUNT          582 a         1450 c         1453 g          521 t
ORIGIN      
        1 gaggatccac gtcgcgccga tcacgatcat gaagccggcg acgatgaaga tcgagaagtt
       61 ctgcttcggc tggccgagga tccactcgtc cgtcggcagg aggaagaacg cgacgagcgc
      121 gaagccggcc gtcgtgcggg cgacgcgctc ggagacgccg agagcgcggg cgaccgggac
      181 gagccccatg atcgcgagcc cgactccgag cccgagcgtc gtcacgtcgt ccgagccgta
      241 gcccgacatc gcgaggagcg cgccaagcgc gatcgcgagc ccgccgaaga gcagacggcg
      301 gcgctgccct ttctgcgcgg gcggctccgg gaggttccgg atcgccgtca cgatgttcat
      361 ccggctgacc cgccaggcgg agaggccgac gacgaggaag gtgaggacga cgccgaccga
      421 gtacgcgacg acgacgctct tcggcgagac ggtgtacgcg atctcgaggt cgctctcggc
      481 ggcgacggcg ctcgcgacga cgagcaccat gacgtaggcg accgcgatcc cgacgagcgc
      541 accgaccgcc gcggcgagga gcgcgtacgt gacgccctcg tagaggaacg tctgcacgag
      601 gtggccccgg cgggtgccga ccgcgcgcgc gatcccgagc tccgcgcgcc gctcggccgc
      661 gagcatcacg aagatgagga agatcaggag cagcccggcg gcgatcgaga acgacccgaa
      721 ggtcgtgaag agcgacatga aggacgcccc gccgaggtcg gccgtctcga gagcgtccgc
      781 cttcgcgggc tcggcctcga gcgcgagccg cgtgagcgtc ggctcgagca gctcgatcac
      841 ctcgtcgctc agcgccgcgc cgctcatggc gtcgccgcgg ttcgagacga gcacgtgccg
      901 gatctggccg ggctcgccga cgaggtcctg ggcgggcccg agcgggacga gcagcgcggg
      961 gccgtccgcc ccggcgccgc ggaacgagac gaccgcctcg acgcgcatcg gcacgaccgt
     1021 ctcgccggcg agcacgcgca gcttgtcgcc gtccgtgacc gcgagctcct cggccgcctc
     1081 ctcgttcacg aagaccgctc cgggcccgag gtcggcgagc gagcgcgtct cgcccgcgag
     1141 gtcgcggatc gggctcatct cggcgagcgc cgccgggtcg ctgccgaaga gggtcgcgcg
     1201 cggctcgctc agccggctct cgacgccctg cacggcgacc ggctcgatca cggccggtgc
     1261 gaccccgtcg acgaggtcgg agccttcgag cgccgccgcg atctcgcggt agtggccctc
     1321 cgggaagtag cggacggcgg ccgcctcgac ctcgccggtc atgagctccg cctccgcgcc
     1381 cgcgacggac acgacctcgt cggtctcgcc gagcgcggtg agcgagctct ggcggatcgt
     1441 gtggctcatc gtgtcgccgg tcgccagggc cgccgcgacg atcgccgtcc cgagcatgag
     1501 cccggtgacg atcagcgcgc tccgcgcagg gcggcggcgg acgttgcgca cgccgagccg
     1561 gacgaacacg cggttgcgca gcgcgaacac gccgagcacc gcgagcgcgc cgagcacgag
     1621 cgccgacagg actagagcga gcgtgccgac cgggatgccg aagagctcct gcatcgccta
     1681 ggccgcggct cccgcgggcg tctcggcgtg gacgacctcg tacagctccc gccccggcgg
     1741 cgagcggaag taggccacgt actgctcgac ctgctcgtcg tacagcccgc tcgcgaggcc
     1801 ggcctcggcg gtctcggcgt cgcgccagag ggtcatgacg agcgccttgc cctccggggt
     1861 cgagagggcg tagacgccct cgaacccgtc ctgctcacgc agcgccggcg cgatgcggct
     1921 ctcgaacacg ccgaccgcgt cggcgagcgg catccgcacc gtgtcgatct ccgccaacgt
     1981 cacccgcgcg accattgctc acctccagaa gctcctgctc gactacctcg ccgtcgagca
     2041 tccggacgat tcggtcggtc ttgcggccga ccccgatgtc gtgcgtgacg atcacgaacg
     2101 agaggcctcg ctcgacgttc agccggcgca tcaggtcggt gatctcggcg gcgttctcgc
     2161 tgtcgaggtc gccggtcggc tcgtcggccc agacgatcgc aggatcgttc acgagcgccc
     2221 gcgcaatggt cacgcgctgg cgctggccgc ccgagagctc gtccgggagg tggtcggccc
     2281 accgctcgag cccgaccatc tcgagcgcct cgacggcgcg tcggcgggcc tcacgcgcgt
     2341 ggacgcgcgc gacgagcagc ggaagctcga cgttctcgac ggaggagagc accggcatca
     2401 ggttgtagaa ctggaagacg aagcccatgc ggcgcgcccg gtagtccgtg cgcgcgcggt
     2461 cggacatccc ggcgagcggg acgccctcga tctcgatctc gcccgcgtcg atcgcgtcca
     2521 ggccggagag gcagttgagc agcgtcgtct tgccgcagcc gctcgggccc atgatcgcga
     2581 ccatctcgcc gcgcgcgacg gccaggtcga cgccgcgcag cgcgcgcacc tcgaccttcc
     2641 ccgtgtcgta cgtcttgtgg actccacgtg cctcgatgat ctgttcggtc gccataccgc
     2701 catcagacac ccgcgcgcga ggcgggccat cgtggcgaac gctggtttcc gcgtccggga
     2761 aatccccgat ggtcagcgct cgacgcgcgc gccgacgacc cgctcggcgt actcgaacgc
     2821 gaggcgctcg agctcggggt cggtcgtcgc gcacgcgccg gcgaggatgg agaccttgaa
     2881 cccgagctcc cgcgcgtcga tcgccgactg gacgacgcac atctccgtcg ccgcgcccgc
     2941 gagcagcagc cgctcgacct cgagctcgcg caggaggacg acgagcggcg tatggtcgaa
     3001 gatcgagtag cgcggcttga ggaggatccg gtcgcccacg cgcggcgcga cctcggcgag
     3061 cgcgtcgccg ccgggcccgt cgagcgcgtc gcggacgagc gccggcgcgt tcgagtccca
     3121 gcgcccgtcg aggtcgttca cgtagacgac gggcgtgccg tccgcgcggg cgcggtcgag
     3181 cgcgtcgacg agccccggta gccgcccgcg gagcgaggcc aggagcgcgt ccccgtcctc
     3241 gtggtcgaag cgctggagca ggtcgatgac gacgagcgcg tcccgcacgg gcggctctct
     3301 acccggatct agtcgtccac gaagccgacg gagacccaga acggcatgcc ggccgtcgtc
     3361 gtctcggtcg cgcggtacgc gtaggggtgg agcgagccgt ccttgccgcg gagcagcgtc
     3421 gtgcccgccg cctcgccgcg gcggcgaagc gccctgtaga ggtcaggcgc gtccgcggcg
     3481 acggcgacgt ccgtcacgtg gagctcgagc agctcctcgc gctcgtagcc cagcacctcg
     3541 cacgcgcggc ggttgacggc gacatagcgg ccgtcgtcgt cggcgacgaa gacgagggcg
     3601 gggcccgtgt cgacggcctc gcccaggagt cccggctgaa tcaggtcggc ggcggcgcga
     3661 acggtcattg gcctgagatt ttacgccgcc gccgacctcg tttctacggc cgacggccgt
     3721 ttccttacgt tcggtctagg tcgtcacgag cgcctccgga acgtgcgcct cgacgaaggg
     3781 ccggaggacg tccgcgaggg tcggctcgcc gtggtcggcg agcacgtacg tcgcgtgccg
     3841 caccgggtgc cgcacgtcga tcacgcgcgt caggttcatc ggcgtgccgg tcacgacgac
     3901 gtcgcagtcg gcggcgtcga tcgtggccgc gagctcggcg agctgctcgt cgccgtagcc
     3961 catcgccggg agcacggccc cgatctccgg gtagcgctcg aacgtc
//
LOCUS       DASTFC010000264         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_368751,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000264 DASTFC010000000
VERSION     DASTFC010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368751"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFO88_07845"
     CDS             complement(<1..839)
                     /locus_tag="VFO88_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9545475.1"
                     /translation="MRALRLILPAVIALVGAAPASAQEPPPPPVIAEGVTISGIAVGG
                     LTRPEASAVVEAAFDVRIPFRFKERRWSASPRALGSSPFLRDATIAALAAEPGANVRL
                     GVKIRPRKIRSYVNFLQRVFGRDARDSRVLLRGTTPFVTKAKDGRAVRKLIMRRAITR
                     ALARTERDELTLQMRVVKPRVNRSNIGPIIVVRRESRRLQLWRGMRLVATFPVAVGTP
                     SYPTPLGRYSVVSKLRNPTWNPPSSPWAAGLGPVPPGPANPLGTRWIGTSAPGIGIHG
                     TPAP"
     gene            complement(886..1599)
                     /locus_tag="VFO88_07850"
     CDS             complement(886..1599)
                     /locus_tag="VFO88_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET9545476.1"
                     /translation="MRPLPSRADGGPLRIGHRGAAALAPDNSLAGIELALDAGIDGVE
                     IDVVAGSGRLLLAHSFSELGGTSPELDDALALVAARAGDGFLLDLDVKTAGAEDALVA
                     ALRRHGLVERTLVSSFHPEVLRTCRMLEPVLRLGISYPDDRRGIGRRLPEPVIRGGLL
                     ALRAALPARIGRLAATSGAGAAMLHHLVLSRATVRRCRRLGLAVFAWTVNDTDALRRV
                     VDLGVDGVIGDDPELLRAA"
     gene            complement(1602..>2462)
                     /locus_tag="VFO88_07855"
     CDS             complement(1602..>2462)
                     /locus_tag="VFO88_07855"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9545477.1"
                     /translation="DGLLYETESSYQYVQVLERSDGSRALRLNEGVAVHSVWHRDSVL
                     TGGVWDTFLLVPPLLDEEPDRMLVVGNAGGTVARAYGELYPEMEIDGVEIDPAVTEAG
                     RRYLGLEDNPRLTVHAADGRPFLELADERWDIIVVDAYHQPYIPFYLATEEFFRLVRD
                     RLEPGGVMALNVAGVPGDESLSEAIGTTVLAAFPQAWRWPALRFNELLLAFDRPLERD
                     ELAARAEDAPGEVASLVPLFAADVEEVRATGDVLTDDVAPVEWLTDRMILDFVARGGE
                     LDERYLPTEP"
BASE COUNT          345 a          930 c          892 g          295 t
ORIGIN      
        1 ggcgccggcg tcccgtggat cccgatgccc ggcgcggacg tgccgatcca gcgcgtgccg
       61 agcgggttcg cggggcccgg cgggaccggc ccgagcccgg ccgcccacgg gctcgacggc
      121 gggttccacg tcggattgcg cagcttcgac acgacgctgt agcggccgag cggcgtcggg
      181 tacgacggcg tcccgaccgc gacggggaac gtcgccacga gccgcatccc gcgccagagc
      241 tgcagccgcc gcgactcgcg gcgcacgacg atgatcggcc cgatgttcga ccggttcacg
      301 cgcggcttca cgacgcgcat ctgcagcgtg agctcgtccc gctccgtccg cgcgagcgcg
      361 cgggtgatcg cgcgccgcat gatcagcttg cggaccgccc gcccgtcctt cgccttcgtc
      421 acgaacggcg tcgtcccgcg caggaggaca cgcgagtcgc gcgcgtcccg gccgaagaca
      481 cgctggagga agttcacgta cgagcggatc ttcctcggcc ggatcttcac gccgagcctc
      541 acgttggccc ccggctcggc cgcgagcgcg gcgatcgtgg cgtcgcggag gaacggcgac
      601 gacccgagcg cgcgcggcga cgccgaccag cgtcgctcct tgaagcggaa ggggatccgc
      661 acgtcgaacg ccgcctcgac gaccgcggac gcctcgggtc gcgtgaggcc tccgaccgcg
      721 atgcccgaga tggtcactcc ctcggcgatg accggcggtg gaggcggctc ctgcgcggag
      781 gccggagccg caccgacgag cgcgatcacg gccggaagga tgaggcgaag ggctcgcacg
      841 ggcgtcgaac tttatacgtg cgacacgccg tttccgagtc ctccttcacg cggcgcgcag
      901 gagctccgga tcgtcgccga tcaccccgtc cacgccgagg tcgacgaccc gtcgaagcgc
      961 gtccgtgtcg ttcaccgtcc aggcgaagac ggcgagcccg aggcgccggc atcgcctcac
     1021 ggtcgcgcgc gagaggacga ggtggtggag catcgccgcg cccgcgccgc tcgtcgccgc
     1081 gagccggccg atccgggcgg gaagcgccgc gcgcagcgcg aggagcccgc cgcggatcac
     1141 gggctccggg agccgccgcc cgatcccccg ccggtcgtcc ggatacgaga tcccgagccg
     1201 caagaccggc tcgagcattc ggcacgtgcg cagcacctcc ggatggaacg acgagacgag
     1261 cgtccgctcg acgagcccgt gccgccgcag cgcggcgacg agcgcgtcct ctgcaccggc
     1321 ggtcttcacg tcgaggtcga gcaggaagcc gtcgcccgcg cgcgccgcga cgagcgcgag
     1381 cgcgtcgtcg agctccgggc tcgttccgcc gagctccgag aacgagtgcg cgaggagcag
     1441 ccgcccgctg ccggcgacga cgtcgatctc gacgccgtcg atccccgcgt cgagcgcgag
     1501 ctcgatcccg gcgagcgagt tgtccggcgc gagcgcggcc gcgccgcggt gcccgatgcg
     1561 gagcggcccg ccgtccgcgc gactcggcaa cgggcgcatc gctacggctc ggtcgggagg
     1621 tagcgctcgt cgagctcccc gccgcgcgcg acgaagtcga ggatcatccg gtcggtcagc
     1681 cactcgaccg gcgccacgtc gtcggtgagc acgtcgccgg tcgcgcgcac ctcctcgacg
     1741 tcggcggcga agagcggcac gaggctcgcc acctccccgg gcgcgtcctc ggcgcgggcg
     1801 gcgagctcgt cccgctcgag cggccggtcg aacgcgagca ggagctcgtt gaagcggagc
     1861 gccggccagc gccacgcctg cgggaacgcc gcgaggacgg tcgtcccgat ggcctccgag
     1921 aggctctcgt cgcccggcac gccggccacg ttcagcgcca tcacgccgcc cggctcgagc
     1981 cggtcgcgca cgagccggaa gaactcctcg gtcgcgaggt agaacgggat gtacggctgg
     2041 tggtacgcgt ccaccacgat gatgtcccac cgctcgtcgg ccagctcgag gaacggccgc
     2101 ccgtcggccg cgtgcacggt caggcgcggg ttgtcctcga gcccgagata gcggcggccc
     2161 gcctcggtca ccgccgggtc gatctcgacc ccgtcgatct ccatctccgg gtagagctcg
     2221 ccgtacgcgc gcgcgaccgt gccgccggcg ttgccgacga cgagcatccg gtcgggctcc
     2281 tcgtcgagga gcggcggcac gagcaggaac gtgtcccaca cgccgcccgt cagcacggag
     2341 tcccggtgcc agaccgagtg cacggcgacg ccctcgttca gcctgagcgc ccgcgagccg
     2401 tccgagcgct cgagcacctg aacgtactgg tacgacgact ccgtctcgta gaggagcccg
     2461 tc
//
LOCUS       DASTFC010000265         6627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_352153,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000265 DASTFC010000000
VERSION     DASTFC010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352153"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFO88_07860"
     CDS             <1..285
                     /locus_tag="VFO88_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545478.1"
                     /translation="RFVTVVRPALRFEQLIETMYSLAADGKTNKKGMPNPLRLAVIAK
                     EHFEDVQLPFPPAFLQRFGLAMGAPVGRMLGYEPTYRPAAPIGDLAGATA"
     gene            353..739
                     /locus_tag="VFO88_07865"
     CDS             353..739
                     /locus_tag="VFO88_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545479.1"
                     /translation="MAETLDPTRRRVRPTPPGALLRGSWLAIKLGVEPRVLDARRRAG
                     ELLAVPEEHGDDHLYPMWQFAPDGTVLPAVTHVLSVARAAGVSEEELYALLGRRHMTG
                     GGRLLDALREGRVEPVLTAINGAARR"
     gene            complement(736..1323)
                     /locus_tag="VFO88_07870"
     CDS             complement(736..1323)
                     /locus_tag="VFO88_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9545480.1"
                     /translation="MSQDSRFPRLVALACHDLRTPLATVHGFARTLVRSDLQEPVDRW
                     VEMIEAASVQLAELLDELSLLARIESGRFEPRLETVDSLVLAREAAGELEDGTVAVDG
                     EGGPVAVEVDATRRALRQLARAARRHGGLESVTVEVRGAELEISPVTAAAEPVVTGSE
                     LRELGAAAAGDLLRALGATVAVADGRLRIRLPEPA"
     gene            1347..2321
                     /gene="mptA"
                     /locus_tag="VFO88_07875"
     CDS             1347..2321
                     /gene="mptA"
                     /locus_tag="VFO88_07875"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HET9545481.1"
                     /translation="MAESVTGAPDLQASPPDIRIGLSRVGVTGVQKAVRMQHEGQDAL
                     VSAEIDCFVDLDSDQKGVHMSRFPELFGEAIDELVIPEKLLVEELAEHIATHIVERQD
                     ACRAEVRIRARYPVERLTPVTGLRTQELVTLIGTAAATETSSRRVVGVEATGINACPC
                     AQGLVREQAVERLGEAGFDGVDVERILELVPLATHNQRGRGLLYLGTRLRVDGEDLVR
                     IVEGSMSSPIYELLKRPDELFVVEHAHLQPRFVEDSVRLMVKGVLDAYPELDDEDFLL
                     ARQVNFETIHNHEVLAERYGTIGELRRALATGEHSARHTELRDWLRAA"
     gene            complement(2318..2926)
                     /gene="plsY"
                     /locus_tag="VFO88_07880"
     CDS             complement(2318..2926)
                     /gene="plsY"
                     /locus_tag="VFO88_07880"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET9545482.1"
                     /translation="MLELLLIPVGYLIGSLPFGYWLPKLFAGVDIRTQGSGNAGATNV
                     WRTFGFKLGLTVALLDIAKGAAAALLARWLADDLIAVFAGLAAMAGHWRPLFLGFGRG
                     GKAVATLGGVGLALAPLPTLAAVGVWIVVFLATRYASLASIAAALFLPLFSLLFEVSV
                     PVLVFTCAAALAVLVLHRTNIRRLRAGTEHRIKLRGRAVAGA"
     gene            complement(2942..3562)
                     /locus_tag="VFO88_07885"
     CDS             complement(2942..3562)
                     /locus_tag="VFO88_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET9545483.1"
                     /translation="MSTLAFNHQEIVARELRPEVLELDGISRASVEAHYKLYQGYVAK
                     RNEILGKLEGIDLSAGNQVYSELRALKVDLTFAIGGVKNHEIYFEHLGGGGGDPDGAI
                     GALIERDFGSVAAWRDDLKATGMAGRGWAWTAYDWDEERLFNYIGDAQNTFPIWNATP
                     LVALDVYEHAYFLDYQTDRASYIDAFFDNLDWRVVNDWVARYRIEI"
     gene            3612..4292
                     /locus_tag="VFO88_07890"
     CDS             3612..4292
                     /locus_tag="VFO88_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9545484.1"
                     /translation="MGHSERAALVTGGTGRVGRAIAARLEADGFRVKAAGRRDGDLAR
                     AAGARELVDTAVAELGRLDVVVHAAAEGFAPQPVEDVTEEDWDAALGATAKGAFFVAQ
                     AAAPHLRAAGDGVIVLVEDVASYEPWPLFAPHSAAKAAEAMLTRVLARALAPEIRVCG
                     VAPGTVTRDGDAGWEERRAAETLLGRVGSPEDVAEAVAYLVHARYVTGTTLVVDGGRL
                     LQRDRPSK"
     gene            4388..4927
                     /locus_tag="VFO88_07895"
     CDS             4388..4927
                     /locus_tag="VFO88_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545485.1"
                     /translation="MATEDDAALIQRTGLGDRDAFEELYRRFARPVLGMAIRQLGDNG
                     RAEDATQETFTAIWRSARSYKPERGTASAWLYAVARHAIIDRARQRVEPAAEAPDEPS
                     RDLGPDEHAELSWLAWRVHSALQRLPERERAVLELAYWSGLSQSEIASYLDVPLGTVK
                     TRTRAGLARLASLLEEVRR"
     gene            4924..5541
                     /locus_tag="VFO88_07900"
     CDS             4924..5541
                     /locus_tag="VFO88_07900"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET9545486.1"
                     /translation="MSERRPTDLTELVGDDIPEAELAELRRVDSLLRSVPPPPQLPDR
                     LAAPVRQESRPPRLWTPRRSLAAVALAAAVAAVAFGLGAWIAGGNDFDERYSVAMSGT
                     FEAPAASAVVRLGEPDDDGNRPIRLEATGLEPLPDGGYYVLWLEKDGEYAGTCGTFAV
                     GAGDAEAEWTVSYDFTDYDAWVVSARVPGDEPADAPRILEAEISF"
     gene            complement(5538..6245)
                     /gene="thyX"
                     /locus_tag="VFO88_07905"
     CDS             complement(5538..6245)
                     /gene="thyX"
                     /locus_tag="VFO88_07905"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET9545487.1"
                     /translation="MAVTTDVIPENTVRVLDHGFVRLDDAMASDLSVVNAARVSFARR
                     KDEMDESDEGLIRFLMRDRHGTPFEHNSFRFHIRSPIFVAREWMRHRVGSFNEFSMRY
                     AKATDDFYVPEADDVRSQVGKPGAYSFEPVSPELAEQTREELRAVYDHAYETYERLVE
                     AGVARELARSVMPVGAYTEFFWTINARSLMNFVSLRAAETAQREIRRYAEACETFLAE
                     KMPVTHAAFVAAGRTAP"
     gene            6235..>6627
                     /locus_tag="VFO88_07910"
     CDS             6235..>6627
                     /locus_tag="VFO88_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9545488.1"
                     /translation="MTATGPSLAPLQVGASGFSYASWKPGFYPADARPEDFLRLYAER
                     LSTVELNNTFYRLPAEEQFARWAEQVPPAFRFAVTMSRRVTGRGRVEGVDTFETSVRA
                     LGERLGPIRIKVPQARDDGFLLLLLGSLA"
BASE COUNT          898 a         2451 c         2399 g          879 t
ORIGIN      
        1 cgcttcgtca ccgtcgtccg ccctgcgctt cggttcgagc agctgatcga gacgatgtac
       61 tcgctcgccg cggacggcaa gacgaacaag aagggcatgc cgaacccgct ccgcctcgcc
      121 gtcatcgcga aggagcactt cgaggacgtg cagcttccct tcccgcccgc cttcctccag
      181 cgcttcgggc tcgccatggg cgcgccggtg ggccggatgc tcggctacga gccgacgtac
      241 cgcccggccg ccccgatcgg cgacctggca ggcgccaccg cctgatcacg ccaaccgccc
      301 ggaggcccgg tcacgccggg cctccgggaa tccgcgtgcg agaatgagcc cgatggcgga
      361 gacactcgat cccacccggc gccgcgtgcg gccgacgccg cccggcgcgc tcctgcgcgg
      421 gtcgtggctc gcgatcaagc tgggcgtcga gccgcgcgtc ctcgacgccc gccgccgcgc
      481 cggcgagctt ctcgcggtcc ccgaggagca cggcgacgac cacctctacc ccatgtggca
      541 gttcgcaccc gacggcaccg tgctgcctgc cgtgacgcac gtcctctccg tcgcccgcgc
      601 cgcgggggtc tccgaggagg agctctacgc cctgctcggc cgccggcaca tgaccggcgg
      661 cggccgcctg ctcgacgcgc tccgcgaggg ccgggtcgag cccgtgctca cggcgatcaa
      721 cggcgccgcg cgccgctagg ccggttccgg caggcggatc cgcagccgcc cgtcggccac
      781 cgcgaccgtc gcgcctagcg cgcgcagaag gtcgcccgcg gcggccgcgc cgagctcccg
      841 aagctccgag cctgtgacga ccggctccgc cgcggcggtg acgggggaga tctcgagctc
      901 ggctccgcgg acctccaccg tcacgctctc gagcccgccg tgccggcgcg ccgcgcgcgc
      961 gagctgccgg agcgcgcgcc gcgtcgcgtc gacctcgacc gcgaccggcc cgccctcgcc
     1021 gtcgaccgcg accgtcccgt cctcgagctc ccccgccgcc tcgcgcgcga gcacgagcga
     1081 gtcgacggtc tcgagccgcg gctcgaaccg gcccgactcg atccgcgcga gcaggctcag
     1141 ctcgtcgagg agctccgcga gctgcaccga cgccgcctcg atcatctcga cccaccggtc
     1201 gacgggctcc tggaggtcgc tccggacgag cgttcgcgcg aagccgtgca ccgtcgcgag
     1261 cggcgtccgg aggtcgtgac acgcgagcgc gacgaggcgc gggaaacgcg agtcctggct
     1321 cacagggacg gtgctacggt tgccacgtgg ccgagagcgt aaccggggcg cccgacctcc
     1381 aggcttcgcc gcccgacatc cgcatcgggc tctcgcgggt cggcgtgacc ggcgtgcaga
     1441 aggctgtgcg catgcagcac gagggccaag acgcgctcgt ctcggccgag atcgactgct
     1501 tcgtcgacct cgattccgac cagaagggcg tccacatgtc gcgcttcccc gagctcttcg
     1561 gggaggcgat cgacgagctc gtgattccgg agaagctcct cgtcgaggag ctcgccgagc
     1621 acatcgccac gcacatcgtc gagcgccagg acgcctgccg ggccgaggtg cggatccgcg
     1681 cccgctaccc ggtcgagcgg ctgacgccgg tgaccggcct gcgcacgcag gagctcgtga
     1741 cgctgatcgg cacggccgcg gcgacggaga cgagctcgcg gcgggtcgtc ggcgtcgagg
     1801 cgaccgggat caacgcctgc ccctgcgcgc agggcctcgt ccgcgagcag gcggtggagc
     1861 ggctcggcga ggcggggttc gacggcgtcg acgtggagcg gatcctcgag ctcgtcccgc
     1921 tcgcgacgca caaccagcgc ggccgcgggc tcctctacct cgggacgcgg ctccgcgtcg
     1981 acggcgagga cctcgtgcgg atcgtcgagg gctccatgag ctcgccgatc tacgagctgc
     2041 tcaagcgccc cgacgagctc ttcgtcgtcg agcacgcaca cctccagccg cgcttcgtcg
     2101 aggactcggt gcgcctcatg gtgaagggcg tcctcgacgc gtacccggag ctcgacgacg
     2161 aggacttcct gctcgcgcgc caggtcaact tcgagacgat ccacaaccac gaggtgctcg
     2221 ccgagcgcta cgggacgatc ggcgagcttc gccgcgccct cgcgaccggc gagcattccg
     2281 cgcgccatac cgagctgcgc gactggctcc gggccgccta ggcgcccgcg accgcgcggc
     2341 cgcggagctt gatgcggtgc tccgtgccgg cgcggagccg gcggatgttc gtccggtgca
     2401 ggacgagcac ggcgagcgcg gccgcgcacg tgaagacgag cacgggcacc gagacctcga
     2461 agaggagcga gaagagcggg aggaagagcg cggccgcgat cgaggcgagg gaggcgtagc
     2521 gggtggcgag gaagaccacg atccagaccc cgaccgcggc gagcgtgggc agcggcgcga
     2581 gcgcgagccc gacgccgccg agcgtcgcga ccgccttgcc gccgcgcccg aagccgagga
     2641 acagcggccg ccagtgcccc gccatcgccg cgagcccggc gaagacggcg atcaggtcgt
     2701 ccgcgagcca gcgcgcgagg agcgccgccg cggcgccctt ggcgatgtcg agcagcgcga
     2761 ccgtgagccc gagcttgaag ccgaacgtgc gccacacgtt cgtcgcgccc gcgttgccgc
     2821 tcccctgcgt ccggatgtcc acgccggcga agagcttcgg cagccagtac ccgaacggca
     2881 gcgagccgat caggtagccg accgggatga gcagcagctc gagcacgccc ccgcccggga
     2941 tctagatctc gatgcggtag cgcgcgaccc agtcgttgac cacgcgccag tcgaggttgt
     3001 cgaagaacgc gtcgatgtag gacgcgcggt cggtctggta gtcgaggaag tacgcgtgct
     3061 cgtagacgtc gagcgcgacg agcggggtcg cgttccagat cgggaacgtg ttctgcgcgt
     3121 cgccgatgta gttgaagagc cgctcctcgt cccagtcgta cgcggtccac gcccagccgc
     3181 ggccggccat cccggtcgcc ttcaggtcgt cgcgccatgc ggcgacgctg ccgaagtcgc
     3241 gctcgatgag cgcgccgatc gcgccgtccg ggtcgccgcc cccgccgccg aggtgctcga
     3301 agtagatctc gtggttcttc acgccgccga tcgcgaacgt gaggtcgacc ttgagcgcgc
     3361 gaagctccga gtagacctgg ttcccggccg agaggtcgat gccctcgagc ttgccgagga
     3421 tctcgttccg cttcgcgacg tagccctggt agagcttgta gtgcgcctcg acggacgcgc
     3481 gggagatgcc gtcgagctcg agcacctcgg gccggagctc ccgggcgacg atctcctggt
     3541 ggttgaaggc gagcgtggac attggcttga gttctcctct gtcgaagtcc gcgggagctt
     3601 atcgtgcccc catgggccat tcggaacgcg cggcgctcgt cacgggcggc accggccggg
     3661 tcgggcgcgc gatcgccgcg cggctcgagg cggacgggtt ccgggtcaag gcggcggggc
     3721 ggcgcgacgg cgatctcgcc cgggcggccg gggcgcgcga gctcgtggac acggcggtcg
     3781 ccgagctcgg gcggctggac gtcgtcgtgc acgccgcggc cgagggcttc gccccgcagc
     3841 cggtcgagga cgtgaccgag gaggactggg acgcggccct cggcgcgacg gcgaagggcg
     3901 ccttcttcgt cgcgcaggcc gccgcgccgc acctccgcgc ggccggggac ggggtgatcg
     3961 tcctcgtcga ggacgtcgcc tcgtacgagc cgtggccgct cttcgcgccg cactccgcgg
     4021 cgaaggcggc ggaggcgatg ctcacccgcg tcctcgcccg ggcgctcgcg cccgagatcc
     4081 gcgtctgcgg cgtggcgccc ggcacggtca cgcgcgacgg cgacgccggc tgggaggagc
     4141 gccgcgccgc cgagaccctc ctaggccggg tcggctcccc cgaggacgtg gccgaggcgg
     4201 tcgcgtacct cgtccacgcg cgctacgtga ccgggacgac gctcgtcgtg gacggcgggc
     4261 ggctgctcca gcgcgaccgc ccgtccaagt aacactgtgt tacaagcaaa ggggctcccg
     4321 ccggccgtgg gcggcaatcc acgctccctt gaacttcgta tctccgcgcg gtatggcttt
     4381 gctcgcgatg gcgaccgaag acgacgctgc gctgatccag cgcacggggc tcggcgatcg
     4441 ggacgccttc gaggagctct accgccgctt cgcgcgcccc gtgctcggga tggcgatccg
     4501 ccagctcggc gacaatggcc gcgcggagga tgcgacccag gagacgttca cggcgatctg
     4561 gcgctccgcc cggagctaca agcccgagcg tggcacggcg tccgcctggc tctacgccgt
     4621 cgcgcggcac gcgatcatcg accgcgcccg ccagcgcgtc gagcccgcgg cggaggcgcc
     4681 cgacgagccg tcccgcgacc tcgggccgga cgagcacgcc gagctctcct ggctcgcgtg
     4741 gcgcgtccac tccgcgctcc agcggctccc cgagcgcgag cgcgcggtgc tcgagctcgc
     4801 gtactggagc ggcctctcgc agagcgagat cgcgagctac ctcgacgtgc cgctcggaac
     4861 cgtgaagacg cgcacgcgcg ccggcctcgc gcgcctcgcg agcctcctcg aggaggtgcg
     4921 gcggtgagcg agcggcgccc gaccgatctc acggagctcg tcggggacga catcccggag
     4981 gccgagcttg cggagctgcg ccgcgtggac tcgctcctgc gcagcgtgcc cccgccgccc
     5041 cagctgcccg accggctggc ggcgccggtg cgccaggaat cgcgcccgcc gcggctctgg
     5101 acgccgcgcc gctcgctcgc cgcggtcgcg ctcgcggcgg ccgtggcggc ggtcgccttc
     5161 gggctcggcg cctggatcgc cggcggcaac gacttcgacg agcgctactc cgtggcgatg
     5221 agcgggacct tcgaggcgcc cgcggcgtcg gcggtcgtcc ggctcggcga gcccgacgac
     5281 gacggcaacc gccccatccg cctcgaggcg accgggctcg agccgcttcc cgacggtggc
     5341 tactacgtcc tctggctcga gaaggacggc gagtacgccg gcacgtgcgg caccttcgcc
     5401 gtcggcgcag gtgacgctga ggccgagtgg acggtctcct acgacttcac cgactacgac
     5461 gcgtgggtcg tgagcgcccg cgtccccggc gacgagccgg cggacgcgcc gcggatcctc
     5521 gaggccgaga tctcgttcta gggggccgtt cggcccgcgg cgacgaaggc cgcgtgggtg
     5581 accggcatct tctcggcgag gaacgtctcg cacgcctcgg cgtaccgccg gatctcccgc
     5641 tgggcggtct cggcggcgcg gagcgacacg aagttcatga gcgagcgcgc gttgatcgtc
     5701 cagaagaact cggtgtacgc gccgaccggc atcaccgacc gcgcgagctc gcgcgcgacg
     5761 cccgcctcga cgagccgctc gtacgtctcg tacgcgtggt cgtagaccgc gcggagctcc
     5821 tcgcgcgtct gctcggcgag ctcgggcgag accggctcga aggagtacgc gccgggcttg
     5881 cccacctgcg agcgcacgtc gtcggcctcc gggacgtaga agtcgtccgt cgccttcgcg
     5941 tagcgcatgg agaactcgtt gaacgagccg acgcggtgcc gcatccactc gcgcgcgacg
     6001 aagatcggcg accggatgtg gaagcggaac gagttgtgct cgaagggcgt cccgtgccgg
     6061 tcgcgcatga ggaagcggat caggccctcg tccgactcgt ccatctcgtc cttccgccgg
     6121 gcgaaggaga cgcgcgcggc gttcacgacg gagaggtcgc tcgccatcgc atcgtcgagg
     6181 cggacgaacc cgtggtcgag cacgcggacg gtgttctcgg gtatcacgtc ggtcgtgacc
     6241 gccaccgggc cgagtctagc gcccctccag gtcggagcct ccggcttctc ctacgcctcg
     6301 tggaaaccgg gcttctaccc tgccgacgct cggccggagg acttcctgcg gctctacgcg
     6361 gagcggcttt cgacggtcga gctgaacaac acgttctacc gcctgccggc cgaggagcag
     6421 ttcgcgcgct gggccgagca ggtgccgccg gccttccgct tcgccgtcac gatgagccgc
     6481 cgcgtgaccg gccgcgggcg cgtcgagggc gtggacacgt tcgagacgag cgtccgagcg
     6541 ctcggcgagc ggctcgggcc gatccgcatc aaggtgccgc aggcccgcga cgacgggttc
     6601 ctccttctcc tgctcggctc gctcgcg
//
LOCUS       DASTFC010000266         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1539052,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000266 DASTFC010000000
VERSION     DASTFC010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539052"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..2777)
                     /locus_tag="VFO88_07915"
     CDS             complement(21..2777)
                     /locus_tag="VFO88_07915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9545489.1"
                     /translation="MTAVSGELTVDGVGAEPEPQGLPRRALVFLGLTGAMTVAAAALP
                     IANLDGREADWLTFVVLAVAAAAAQLFVARTPRNAARYYTTIVFLIAAVLLLPAGLVA
                     LMGVIQHVPEWLKMRHPWYIQTFNIANYTLNCLAAWYAAHFVLAGASAADAPERWAAA
                     GAVACVVFVVTNHVLLAVMMHVAERTPLRKSELFTLESLSTDLILALLGVAFATLWEI
                     NTWLIVAAVAPLVLIHRSLNVPALEEEARVDAKTGLFNARHFAAELREELARAERFDR
                     PMSVIMADLDLLRDINNAYGHLAGDAVLRGIADTFRAALRDYDVPARFGGEEFAILLP
                     ETPPERALEIAERIRRTVAATRFEVATSSEPIRATISCGVAGYPKDGTDVNELIHQAD
                     LAVYRAKLQGRNRALGASSEPLLMPAERSARLAAVPEEPVDLPSAVPLPVEAPAAGGP
                     DRRQSAPRRHMPVGPRFVALSPLLGSFVAVVALAGAAAGVLGLFLGGTEDFVGLLAIV
                     LAVGIGQALSLELEGGSVSVSAVGALAGAAMFGPRAALPLAVTMAAVQWSAMRNPLHQ
                     SLFNVGTLCVASLSAAAVFSIGWGDDAIGHLVTALAGLVAGGLYFVINTGLLSGAMAL
                     EGHDRWARVWKERFSWLGPHYVAFGAVAAAMALAYEAIGLYGLAVFALPLLLMRKTMA
                     AYIGHTARSSSKLREAAETIRNQNASLEQANRLLKERSTAAMESLSATVDARDAYTAG
                     HSRRVQQLSLAIGRELGLSQAELDLLGHAALFHDIGKLAIPDAVLLKPAELTGDEWSL
                     MQRHAEEGARIIDRLGFLQDAVPAIRHHHERFDGAGYPDGLGGEEIPLGARIIHVADA
                     LDSMLTTRIYRAARPVADAVAELRIGSGTQFCPRCVGALERLVEADGLADYGIRVPVL
                     ANAS"
     gene            2975..>3789
                     /locus_tag="VFO88_07920"
     CDS             2975..>3789
                     /locus_tag="VFO88_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET9545490.1"
                     /translation="MRLRLEIGLARLAGRLSRLAARGGGTTLPGKLLVAVDPGALATL
                     AERLPHGAALVSATNGKTTTAAMTAEILGRRVRLAHNRAGANLVSGVASALLDADGAE
                     LGLFEVDEAAFPEVARRVRPRVVTLGNLFRDQLDRYGELELVAERWRDALASLPADTV
                     AVVNADDPLVAGLARGREAALRFGLDDPSVARERLPHAADSKYCVSCGTPYEYAAAYV
                     GHLGAYRCPRGDDERPPLDVAARSISLEGLERSSFDLVTPQGTRRVTVGLPGL"
BASE COUNT          511 a         1375 c         1431 g          472 t
ORIGIN      
        1 ccgtatcgtc gcaggagccg ttaggaggcg ttcgcgagaa ccgggacgcg gatcccgtag
       61 tccgcgaggc cgtcggcctc gacgaggcgc tcgagcgcgc cgacgcagcg cgggcagaac
      121 tgcgtgcccg agccgatgcg aagctccgcg accgcgtcgg cgaccgggcg cgcggcgcgg
      181 tagatccggg tcgtcagcat cgagtcgagc gcgtcggcga cgtggatgat gcgggcgccg
      241 agcgggatct cctcgccccc gagcccgtcc gggtacccgg ccccgtcgaa gcgctcgtgg
      301 tggtggcgga tggccgggac cgcgtcctgg aggaagccga gccggtcgat gatccgcgct
      361 ccctcctccg cgtggcgctg catgagcgac cactcgtcgc ccgtgagctc ggccggcttc
      421 aggaggacgg cgtccgggat cgcgagcttg ccgatgtcgt ggaagagcgc cgcgtgaccg
      481 agcaggtcga gctccgcctg cgagagcccg agctcgcggc cgatcgcgag cgagagctgc
      541 tgcacccgcc gcgagtggcc ggcggtgtac gcgtcgcggg cgtccacggt cgcggagagg
      601 ctctccatcg cggcggtcga gcgctccttg aggagtcggt tcgcctgctc gagggaggcg
      661 ttctggttcc ggatcgtctc ggccgcctcg cggagcttcg agctcgagcg ggcggtgtgg
      721 ccgatgtacg cggccatcgt cttgcgcatg agcaggagcg ggagcgcgaa gaccgcgagg
      781 ccgtagaggc cgatcgcctc gtacgcgagc gccatcgccg ccgcgaccgc gccgaacgcg
      841 acgtagtgcg gcccgagcca cgagaagcgc tccttccaga cgcgcgccca cctgtcgtgt
      901 ccctcgagcg ccatcgcgcc ggagaggagc cctgtgttga tcacgaagta gagcccgccc
      961 gccacgaggc ccgcgagcgc cgtcacgagg tggccgatcg cgtcgtcacc ccagccgatc
     1021 gagaagaccg cggcggccga gagggaggcg acgcagagcg tcccgacgtt gaagagcgac
     1081 tggtggagcg ggttccgcat cgcgctccac tggacggccg ccatggtgac ggcgagcggg
     1141 agcgcggcgc gcgggccgaa catcgccgcg ccggcgagcg cgccgaccgc gctcacggac
     1201 acggagcctc cctccagctc gagcgagagc gcctgcccga tcccgacggc gaggacgatc
     1261 gccaggaggc cgacgaagtc ctccgtcccg ccgaggaaga gcccgagcac gcccgcggcc
     1321 gcgccggcca gcgcgacgac ggcgacgaac gacccgagga gcggcgacag ggcgacgaag
     1381 cgcggcccga ccggcatgtg ccgccggggc gcgctctggc ggcggtccgg ccctccggcg
     1441 gcgggcgcct ccacggggag cggaaccgcc gagggaagat cgacgggctc ctccggcacg
     1501 gcggcgaggc gtgcgctccg ctcggcgggc atgaggagcg gctcggagct cgccccgagc
     1561 gcgcggttcc ggccctggag ctttgcgcgg tagaccgcga ggtctgcctg gtggatgagc
     1621 tcgttgacgt ccgtcccgtc cttcgggtac ccggcgacgc cgcacgagat cgtcgcgcgg
     1681 atcggctcgc tcgacgtcgc cacctcgaac cgcgtcgcgg cgaccgtccg ccggatgcgc
     1741 tccgcgatct cgagggcgcg ctccggcggc gtctcgggga gcaggattgc gaactcctcc
     1801 ccgccgaagc gcgccggcac gtcgtagtcg cggagcgcgg cccggaacgt gtcggcgatc
     1861 ccgcgcagga ccgcgtcgcc ggcgagatgg ccgtacgcgt tgttgatgtc ccgcaggaga
     1921 tcgaggtcgg ccatgatcac ggacatcggc cggtcgaagc gctcggcgcg cgcgagctcc
     1981 tcgcgcagct ccgcggcgaa gtggcgggcg ttgaagagcc ccgtcttcgc gtcgacgcgc
     2041 gcctcctcct cgagcgcggg gacgttgagc gagcggtgga tgaggacgag cggggcgacg
     2101 gccgcgacga tgagccacgt gttgatctcc cagagcgtgg cgaacgcgac gccgaggagg
     2161 gcgaggatga ggtcggtcga gaggctctcg agcgtgaaga gctcgctctt gcggaggggc
     2221 gtccgctcgg ccacgtgcat catcaccgcg agcaggacgt ggttcgtgac gacgaagacg
     2281 acgcacgcga ccgcgcccgc cgccgcccac cgctccggcg cgtccgccgc gctcgcaccc
     2341 gcgaggacga agtgcgccgc gtaccacgcc gcgaggcagt tcagcgtgta gttcgcgatg
     2401 ttgaacgtct ggatgtacca ggggtggcgc atcttcagcc actccggcac gtgctggatc
     2461 acgcccatga gcgcgacgag ccccgccggc aggagcagca cggccgcgat gaggaagacg
     2521 atcgtcgtgt agtagcgggc cgcgttgcgc ggcgtccgcg cgacgaagag ctgtgcggcc
     2581 gcggccgcga cggcgaggac gacgaacgtg agccagtccg cctccctccc gtcgaggttc
     2641 gcgatcggga gcgcggccgc cgcgaccgtc atcgcgccgg tcaggccgag gaagacgagc
     2701 gcccgccgtg ggagcccttg cggctccggc tccgcgccga cgccgtcgac cgtcagctca
     2761 ccggagacgg ccgtcatcgt gcccctcgcc ggagccgtcg ccgaccgtcc gcactcgtcg
     2821 ccatccgtct catcacactc cacgcagtcg cttgcgcggg ccgcccgtgt gccctggttg
     2881 caggctaggg cggccgaaac ggcgcggcat cccccgtccg gggtacgagc cgagcggccc
     2941 aaacccctga aacgggggaa ctacactggg ccgggtgcgg ctccgcctcg agatcggcct
     3001 cgcgcggctc gccggccgcc tctcgcgcct ggccgcgcgc ggcggcggga cgacgctccc
     3061 gggcaagctc ctcgtggccg tcgacccggg cgcgctcgcg accctcgcgg agcggcttcc
     3121 gcacggcgcc gcgctcgtct cggcgacgaa cgggaagacg acgacggccg cgatgaccgc
     3181 cgagatcctc gggcggcgcg tccggctcgc gcacaaccgg gcgggcgcga acctcgtgtc
     3241 cggggtcgcc tctgcgctcc tcgacgcgga cggcgcggag ctcgggctct tcgaggtgga
     3301 cgaggccgcc tttccggagg tggcccggcg cgtgcggccg cgcgtcgtca cgctcgggaa
     3361 cctcttccgc gaccagctcg accgctacgg cgagctcgag ctcgtggccg agcggtggcg
     3421 cgacgcgctc gcctcgctcc cggcggacac ggtcgcggtc gtgaacgcgg acgacccgct
     3481 cgtggccggg ctcgcgcgcg ggcgcgaggc cgcgctccgg ttcgggctcg acgatccgtc
     3541 ggtcgcccgc gagcgcctcc cccacgccgc cgactcgaag tactgcgtct cctgcggcac
     3601 gccgtacgag tacgcggccg cctacgtggg ccacctcggc gcgtaccgct gcccccgcgg
     3661 cgacgacgag cggccgccgc tcgacgtggc ggcgcgctcg atctcgctcg aggggctcga
     3721 gcgctcgtcc ttcgacctcg tgacgccgca gggaacgcga cgggtgacgg tcgggctgcc
     3781 cggcctcta
//
LOCUS       DASTFC010000267         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_566813,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000267 DASTFC010000000
VERSION     DASTFC010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566813"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFO88_07925"
     CDS             complement(<1..394)
                     /locus_tag="VFO88_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545491.1"
                     /translation="MRRAVVVVLAGVLFLGACGGDEDDAADTSAASPTAPRGEPIVIQ
                     TSVTIAAEEGAEPIATGEVLEGSTLAGSPFCAGGTILDSHASGDPAVEPYGLIDRTIT
                     CPDGTVRAAFTPDVDVPPGQTQTGSWTIV"
     gene            complement(499..571)
                     /locus_tag="VFO88_07930"
     tRNA            complement(499..571)
                     /locus_tag="VFO88_07930"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(536..538),aa:Ala,seq:ggc)
     gene            722..967
                     /locus_tag="VFO88_07935"
     CDS             722..967
                     /locus_tag="VFO88_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545492.1"
                     /translation="MRRDRALPGRLFVPVLPRNALRARPLAAVLPRRALTVPTVDPDP
                     ETRRRARVQSPAIGFVGALLLLAALLAVVALVVWAVL"
     gene            complement(964..1317)
                     /gene="rsfS"
                     /locus_tag="VFO88_07940"
     CDS             complement(964..1317)
                     /gene="rsfS"
                     /locus_tag="VFO88_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET9545493.1"
                     /translation="MTPLEQAQRIAAIAQEKLGRDVSILDMRPVCSYTDYFVLCTGNN
                     PRQTKAIYDEVHARLKKEERLLPHSVAGLDQSTWILADYLDVVLHVFTPDARDYYRLE
                     DLWGDVPSVEPAASA"
     gene            complement(1389..1976)
                     /gene="nadD"
                     /locus_tag="VFO88_07945"
     CDS             complement(1389..1976)
                     /gene="nadD"
                     /locus_tag="VFO88_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HET9545494.1"
                     /translation="MSVVALLGGTFDPPHVGHVALARAALAELPVDRVVVVPLGEAPH
                     KAVETDAGTRLRLAEAAFADEPRVEVSRHELERPGPSYTVDTARWAAAELGDPIFLVG
                     ADEFADFLTWREPETILELARVAVATRRGSDAARLDRVRTRLARPDRVLELPFTPLDV
                     SSTEIRERVAKGEPIDGLVPPAVARLVAELGLYRP"
     gene            complement(2077..>2938)
                     /locus_tag="VFO88_07950"
     CDS             complement(2077..>2938)
                     /locus_tag="VFO88_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET9545495.1"
                     /translation="GLGGYGNKRFASPTEQAPRVAEVGQAGEEGTFELRLKLLADAAL
                     LGFPNAGKSSLLRRISNAKPKVAEYPVTTLAPVLGTVEAPDGRQLTVADVPGLVEGAS
                     EGIGLGDEFLAHLERARLFLHVIDVTEDAAERFTAIDGELAAYGAGLAERPQLVVLNK
                     IDLVPEPPPFPLADPRVVGVVPVSAATGAGIDALRRALFEAAAAAAPPDGAPAGGPEE
                     DELADFLVYRPQPPRARPFRIFRGDRGFRVSGPAAEGLAADELREALRRAGARPGDQV
                     SVGERTYEFS"
BASE COUNT          384 a         1095 c         1078 g          381 t
ORIGIN      
        1 cgacgatcgt ccacgagccc gtctgggttt gacccggcgg cacgtccacg tccggcgtga
       61 aggcagccct gacggtgccg tccgggcagg tgatcgtccg gtcgatcagg ccgtacggct
      121 ccacggcggg atcgccgctc gcgtgggaat ctaggatggt cccgccggca cagaacggcg
      181 atcccgcgag ggtggacccc tcgagaacct cgccggtggc gatcggctca gcgccctcct
      241 ccgcggcgat cgtcaccgac gtctggatca cgatcggctc gccgcgtggc gcggtgggcg
      301 aggccgcgct cgtgtccgcc gcgtcgtcct cgtcaccgcc gcacgccccc aggaagagca
      361 cacccgccag gaccaccacg accgctctgc gcatgaccgc tccctcgagc tctgacgagc
      421 acgacccgcg ccagtatccc tctttgtggg ggacgtcggc aagggcgcgc gactacctcg
      481 agacgccgcg atccccgatg gagccagccg ggctcgaacc ggcgacctcc acgctgccag
      541 cgtggcgctc tcccagctga gctatggccc cgagcaggga cacgatagcg caccgtgctc
      601 ggcgcctctc ccgtttcgcg ggcgcggcgg cgggtaggcg cgagacagtg accgagccgc
      661 acgcaccgaa gggccacacg agccgcgcgc tcgccgcggc ggcgaggctg cgctgcacgg
      721 catgcggcga gaccgggcgc ttccgggccg gctcttcgtg cccgtcctgc cgcggaacgc
      781 gcttcgagct cgtcccctgg cggccgttct cccgcgccgc gcactgaccg tgccgaccgt
      841 cgatccggat ccggagaccc gccgccgcgc acgcgtccag agcccggcga tcgggttcgt
      901 cggggcgctc ctcctcctcg ccgcgctcct cgcggtcgtc gcgctcgtcg tctgggcggt
      961 gctctaggcc gacgcggccg gctcgacgga cggcacgtcg ccccagaggt cctcgagccg
     1021 gtagtagtcg cgtgcgtcgg gcgtgaagac gtgcaggacg acgtcgaggt agtccgcgag
     1081 gatccacgtc gactggtcga gccccgcgac cgagtgcggg aggaggcgtt cctccttctt
     1141 caggcgggcg tgcacctcgt cgtagatcgc tttcgtctgc cgcgggttgt tcccggtgca
     1201 gagcacgaag tagtccgtgt acgagcacac gggccgcatg tcgaggatgg agacgtcgcg
     1261 gccgagcttc tcctgggcga tcgccgcgat gcgctgcgct tgctcgagcg gggtcagcgg
     1321 gccagcggct ccgtatggat tcctgccatc gggctcaggg tagcccgcgg cggggagggc
     1381 ggcaaccgct acgggcggta gaggccgagc tcggccacga gacgcgcgac cgccggtggg
     1441 acgaggccgt cgatcggctc gcccttcgcg acgcgctcgc ggatctccgt cgacgagacg
     1501 tcgagcggtg tgaacgggag ctccagcacg cggtccggcc gcgcgagccg ggtgcggacg
     1561 cgatcgaggc gcgccgcgtc ggagccgcgt cgggtggcca ccgcgacgcg tgcgagctcg
     1621 aggatcgtct cgggctcgcg ccatgtgagg aagtcggcga actcgtcggc gccgacgagg
     1681 aagatcggat cgccgagctc ggccgccgcc cagcgggcgg tgtcgaccgt gtacgacggg
     1741 ccgggccgct cgagctcgtg gcgcgagacc tcgacgcgcg gctcgtccgc gaacgcggcc
     1801 tccgcgagcc ggaggcgcgt ccccgcgtcg gtctcgaccg ccttgtgcgg cgcctcgccg
     1861 agcggaacga cgacgacgcg gtcgaccggg agctcggcga gcgccgcccg cgcgagcgcg
     1921 acgtgcccga cgtgcggcgg gtcgaacgtg ccgccgagga gcgcgacgac gctcacgtga
     1981 cctcccccgg gggacccccc tgagttaccc ctcgccgatc agcgtagccg gggaaaccgc
     2041 acgcgtgtgt gacgcttcgt gccgagagtg tgacgatcac gagaactcgt acgtccgctc
     2101 gccgacgctc acctggtcgc cgggccgcgc gccggcgcgg cgcagggcct cgcgaagctc
     2161 gtcggcggcg agcccctcgg ccgccggccc gctcacgcgg aagccgcggt cgccgcggaa
     2221 gatccggaac ggacgggcgc gcggcggctg cgggcggtag acgaggaagt ccgcgagctc
     2281 gtcctcctcg ggcccgccgg ccggcgcgcc gtcgggcggc gcggccgccg cggccgcctc
     2341 gaacagtgcc cgccggagcg cgtcgatccc ggcaccggtc gcggccgaca ccgggacgac
     2401 tcccacgaca cgcggatccg cgagcggaaa cggcggaggc tcaggcacga gatcgatctt
     2461 gttcaggacg acgagctgcg gccgctcggc gagcccggcg ccgtacgccg cgagctcgcc
     2521 gtcgatcgcg gtgaaccgct ccgccgcgtc ctcggtcacg tcgatcacgt gcaggaagag
     2581 ccgcgcccgc tcgaggtgcg cgaggaactc gtcgccgagc ccgatgccct cgctcgcccc
     2641 ctcgacgagc cccggcacgt ccgcgacggt gagctgccgc ccgtccggcg cctcgaccgt
     2701 cccgagcacc ggcgcgagcg tcgtgacggg atactcggcc accttcggct tcgcgttcga
     2761 gatccgccgc aggagggacg acttgcccgc gttcggaaag ccgaggagcg cggcgtcggc
     2821 gaggagcttg aggcgcagct cgaacgtccc ctcctccccg gcctggccga cctccgccac
     2881 gcgcggcgcc tgctcggttg gcgacgcgaa ccgcttgttc ccgtacccgc cgaggccg
//
LOCUS       DASTFC010000268         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1621542,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000268 DASTFC010000000
VERSION     DASTFC010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621542"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..842
                     /locus_tag="VFO88_07955"
     CDS             186..842
                     /locus_tag="VFO88_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9545496.1"
                     /translation="MGLIAIDISVSVDGFVAGPNATLEEPLGEGGERLHEWIFGLDAW
                     RERHGLEGGERSVDNDVVEAAVARWGAVVMGRKMWSGGSGPWEDDPRADGWWGGEPPF
                     RVPVFVLTHHAREPEVKASTPIEFVTDGIEVALGRALAAAGSRDVLVAGGASVAQQAL
                     ATGLADELHLHLAPVLLGGGVRLFTDGVTATTLERESVIESPAVTHVRYRVRHGPGDA
                     "
     gene            complement(901..1260)
                     /locus_tag="VFO88_07960"
     CDS             complement(901..1260)
                     /locus_tag="VFO88_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545497.1"
                     /translation="MADVHKLSEHIIDFAERLEGVADAAQGKGTGRGGLGARWIILPA
                     AGAGLFALARSGAFTRQAKDVMEQAKSRASELPEDLIGRVRQTPQRSSSGNGGQNRRR
                     TSSTRKSSSRKSASSSR"
     gene            complement(1356..3014)
                     /locus_tag="VFO88_07965"
     CDS             complement(1356..3014)
                     /locus_tag="VFO88_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET9545498.1"
                     /translation="MSRKRTSDLWWKNAVVYCLDVETFLDWNGDGHGDIVGLTERIDY
                     LAGLGVSCIWLMPFYPSPRQDDGYDITDFYGIDERAGSPGDFAELIRTANDRGVRVIA
                     DLVVNHTSDRHPWYRAARSSPDSPFRDFYVWSEKKPKEPKGGVVFPDKEDSNWDYDEK
                     AGAWYLHRFYKHQPELNIANPEVRDELAQIMGYWLQQGLAGFRVDAVPFLLEPIGLPD
                     GAVSDPHELMRDLRRYAARHRGDVMLLGEVNLPPKDLRAFFGDEDGDELHMCFNFPVM
                     QAMYLALARGDATPLAAALRELPPIPEDSQWANFVRNHDELTLDQLTDEERAEVFEAF
                     GPEEEHQLFGRGLRRRLPTMLNGDGRRIRMVYSLMFSLPGTPVLFYGEEIGMAENLKI
                     EGRMSVRSPMQWSSERHGGFTTGRRPGRPLVDAPGWGPDRINVSDQRRDDGSLLNWME
                     RLIRRRRECPELGWGACTVLDAGEPAVLAHRCDWDGNVVVAVHSFSESPLEVEVPLDG
                     AEAAVDLFAHEELRPGGKGAVALALEPYGHRWLRLRHEGQRLPP"
     gene            complement(3011..>3724)
                     /locus_tag="VFO88_07970"
     CDS             complement(3011..>3724)
                     /locus_tag="VFO88_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9545499.1"
                     /translation="LAQAGATLAEMFPGRFWMALGSGEYANEHITGEPWPDKSRRNER
                     LAECVEIIRSLLAGEVVDHDGLVRVDRARLWSLPDEPPRLFGPAVSEETARWCGSWAD
                     GLVTVNQPHDRLRAVLDAFREGGGEGKPAALQVHLAYAETDEKALAIAHDQWRTMVYG
                     PPLSWDLATVEQFDQVGTRVRPEDLHDTLLASSDLARHAQSLAEYAELGFDELYLHHV
                     GQEQRAFVDAFGREVLPRL"
BASE COUNT          496 a         1326 c         1361 g          541 t
ORIGIN      
        1 tccccacccc gaacgtcttc accggcggcc aggactacca ctcccgccac gagtggctct
       61 gcgtcgccga cctcggcgcc gccgccgcga cggtcgtcga gctcgtacgg gtctgggccg
      121 agcgtcagcc gcccgagtag cgccgcgccg atgacttccg ctgcttgcgg aggtctgacg
      181 acacgatggg actgatcgcg atcgacatct ccgtctcggt ggacggcttc gtcgccgggc
      241 cgaacgcaac gctcgaggag ccgctcggcg agggcgggga gcggctccac gagtggatct
      301 tcggcctcga cgcgtggcgc gagcgacacg ggctcgaggg gggcgagcga agcgtcgaca
      361 acgacgtcgt cgaggcggcg gtcgcgcgct ggggcgcggt cgtgatgggg cggaagatgt
      421 ggagcggcgg gtcggggccg tgggaggacg acccgagggc ggacggctgg tggggcggcg
      481 agccgccctt ccgcgtgccg gtcttcgtcc tcacgcacca cgcgcgcgag cccgaggtga
      541 aggcctcgac cccgatcgag ttcgtgaccg acgggatcga ggtcgcgctc ggccgggcgc
      601 tcgcggccgc cggctcacgg gacgtgctcg tcgcgggcgg cgcctcggtc gcccagcagg
      661 cgctcgcgac gggcctcgcc gacgagctcc atcttcacct cgcgcccgtc ctgctcggcg
      721 gcggggttcg cctgttcacg gacggtgtga cggcgaccac gctcgagcgg gagagcgtga
      781 tcgagtcacc cgcggtcacg cacgtgcgct accgcgttcg ccacggcccg ggcgacgcct
      841 gacgtcggtc gggtacctgc gccgcccgcc cgtcgcggag ggcggcgcag gcgagctcgg
      901 tcagcgcgac gaggacgccg acttgcgcga ggagctcttg cgggtcgagc tcgtccggcg
      961 gcggttctgg ccgccgttcc ccgaggagga ccgctgcggc gtctggcgga cgcggccgat
     1021 caggtcttcg ggcagctccg aggcgcgcga cttcgcctgc tccatcacgt ccttcgcctg
     1081 gcgcgtgaag gcgccgcttc gcgcaagcgc gaagagcccc gcgccggccg ccgggaggat
     1141 gatccagcgc gcgccgagtc cgccccggcc ggtgcccttg ccctgtgccg catcggcaac
     1201 gccttcgagc cgctcggcga agtcgatgat gtgctctgaa agcttgtgaa cgtcagccat
     1261 ggtgttccgt gcccccttgg atatctttgg ttttggacga cgtttccagc tcgggcggcc
     1321 gcaaaacgcg agaaacgccc gctccaggct ctccgtcagg gcggaagccg ttggccctcg
     1381 tgccggagcc ggagccagcg gtggccgtac ggctcgagcg cgagcgcgac cgctcccttc
     1441 ccgcccggcc ggagctcctc gtgagcgaag aggtccacgg ccgcctcggc cccgtcgagc
     1501 gggacctcga cctcgagcgg cgactcggag aaggagtgga cggcgacgac gacgttcccg
     1561 tcccagtcgc agcggtgtgc gaggacggcc ggctcgccgg cgtcgagaac ggtgcacgcg
     1621 ccccacccga gctccgggca ctcgcgccgc cggcggatga gccgctccat ccagttgagc
     1681 agcgacccgt cgtcgcgccg ctgatcggag acgttgatcc ggtccggccc ccagcccggc
     1741 gcgtccacga gcggccggcc gggccgccgc ccggtcgtga acccgccgtg gcgctcgctc
     1801 gaccactgca tcggcgagcg gacggacata cgcccctcga tcttcaggtt ctccgccatc
     1861 ccgatctcct cgccgtagaa gagcaccggc gtgccgggca gcgagaacat gaggctgtag
     1921 accatccgga tccgccgccc gtcgccgttc agcatcgtcg ggagccggcg ccggaggccg
     1981 cgcccgaaga gctggtgctc ctcctccggg ccgaacgcct cgaagacctc ggcgcgctcc
     2041 tcgtccgtca gctggtcgag cgtcagctcg tcgtggttgc gcacgaagtt cgcccactgc
     2101 gagtcctcgg ggatcggcgg cagctcgcgg agcgccgccg cgagcggcgt cgcgtccccg
     2161 cgcgcgagcg cgaggtacat cgcctgcatg accgggaagt tgaagcacat gtggagctcg
     2221 tcgccgtcct cgtcgccgaa gaacgcccgc aggtccttcg gcgggaggtt cacctcgccg
     2281 agcagcatca cgtcgccgcg gtggcgggcg gcgtagcggc gcaggtcgcg catgagctcg
     2341 tgcggatcgg agacggcgcc gtcgggcagg ccgatcggct cgagcaggaa cgggacggcg
     2401 tcgacccgga agcccgccag cccctgctgg agccagtacc ccatgatctg cgcgagctcg
     2461 tcccgcacct cggggttcgc gatgttgagc tccggctggt gcttgtagaa gcggtggagg
     2521 taccaggcgc cggccttctc gtcgtagtcc cagttcgagt cctccttgtc ggggaagacg
     2581 acgccgccct tcggctcctt cggtttcttc tccgaccaga cgtagaagtc gcggaagggc
     2641 gagtccgggc tcgagcgcgc ggcgcggtac cacgggtgcc ggtccgaggt gtggttcacg
     2701 acgaggtcgg cgatcacgcg aacgccgcgg tcgttcgcgg tgcggatgag ctcggcgaag
     2761 tcgcccggcg agccggcgcg ctcgtcgatg ccgtagaagt cggtgatgtc gtagccgtcg
     2821 tcctgccggg gcgaggggta gaacggcatg agccagatgc acgagacgcc gagcccggcg
     2881 aggtagtcga tccgctcggt cagcccgacg atgtcgccgt ggccgtcgcc gttccagtcg
     2941 aggaacgtct cgacgtcgag gcagtagacg acggcgttct tccaccagag gtcgctcgtc
     3001 cgcttgcggc tcacaggcgc gggagcacct cccggccgaa cgcgtcgacg aaggcccgct
     3061 gctcctggcc cacgtggtgg aggtagagct cgtcgaaccc gagctcggcg tactcggcga
     3121 gcgactgtgc gtgccgcgcg aggtcggacg acgcgagcag cgtgtcgtgg aggtcctccg
     3181 gccggacgcg cgtcccgacc tggtcgaact gctccaccgt cgcgaggtcc caggagagcg
     3241 gcgggccgta gaccatcgtc cgccactggt cgtgcgcgat cgcgagcgcc ttctcgtcgg
     3301 tctccgcata cgcgaggtgc acctggagcg cggccggctt gccctcgccc ccgccctccc
     3361 gaaaggcgtc gaggacggcg cggagcctgt cgtgcggctg gttcaccgtg acgagcccgt
     3421 cggcccagct cccgcaccag cgcgcggtct cctcgctgac ggccgggccg aagagccgcg
     3481 gcggctcgtc cgggagcgac cagagccttg cacggtcgac gcggacgagc ccgtcgtggt
     3541 cgacgacctc gcccgcgagg agactgcgga tgatctcgac gcactccgcg agccgctcgt
     3601 tgcggcggct cttgtccggc cacggctcgc cggtgatgtg ctcgttcgcg tactcgcccg
     3661 agccgagcgc catccagaag cggcccggga acatctccgc gagcgtcgcc cccgcctggg
     3721 cgag
//
LOCUS       DASTFC010000269         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_500973,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000269 DASTFC010000000
VERSION     DASTFC010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500973"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFO88_07975"
     CDS             complement(<1..471)
                     /locus_tag="VFO88_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET9545500.1"
                     /translation="MSGSYEDAGVSLAAAESVVDRLRAAADSTRTAAVVGGLGGFAGL
                     YALDDERLLAASTDGVGTKLVLARRAGRLRDAGRDLAAHCVNDVLTTGADPLFFLDYV
                     AAHRLDLEQVAELVEGAADVCREAGCALLGGETAEMPGVYRDEELDFAGTCVGVV"
     gene            complement(468..1136)
                     /locus_tag="VFO88_07980"
     CDS             complement(468..1136)
                     /locus_tag="VFO88_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545501.1"
                     /translation="MPLRRFLYRGPAGVAWAAAVHLFHYHLVTRKVRLVEARYLGRLG
                     FDLIARYGWIGEGTHWFEPGKSWEELDAMGFRLRLVELGRGAVNVVLQPGHWEVPRVD
                     HLGVALDEEGYEEVLDRASDLGLKIQDHPGKRTFISTDAGYRLEVHPPRDWIEAALDS
                     EEELRVAELRLRSDDPEGKAAALARVLGLEPDGPTVTIGDSLVSFEDGGPEGRPELVG
                     EALA"
     gene            1219..1410
                     /locus_tag="VFO88_07985"
     CDS             1219..1410
                     /locus_tag="VFO88_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545502.1"
                     /translation="MPPLSAPNMEFVVYIVALLGLGLITALAETVVWSQFVSVGGWIT
                     AAYLVSRGFAKMGRASDTP"
     gene            1541..2887
                     /locus_tag="VFO88_07990"
     CDS             1541..2887
                     /locus_tag="VFO88_07990"
                     /inference="COORDINATES: protein motif:HMM:NF005102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9545503.1"
                     /translation="MTVAPDHLWRHFTPAGAAPIVIDRAEGCHIYDTEGNRYLDALAA
                     LYCVNIGYGPYPEIAEAARAQLERLPFFTNWVGFATPPALELTEKLSELVPIDVGRIF
                     LVSGGSEAVEAALKVARQYHRLRGEPTRYKYITRRSAYHGTTMGAISINGSPSLRAQF
                     EPLLPGCLRAPMPYRYRCPYCSDKSACTLRCADEVDDIIRNEDPSTVAAVIMEPVQNS
                     GGSIVPPEGYFQRIREICDAHGVLLVADEVICGFGRVGDWFGTTRYGIEPDMMTMAKG
                     LTSAYAPLGALVASTKVIEPFFSDPKTMLIHGITFGGHPLSCAIALANLEVFERDDLI
                     GHVREMTPVFQEEFGRLADEHPMIGDLRGDGCFYSFELVKDKETKATFTPAERDELIK
                     TFFVPRARELGIYMRVDDRAETAAQFSPPLVAGREELDEFLGVFRQVLDEAYERFV"
     gene            2898..>3679
                     /locus_tag="VFO88_07995"
     CDS             2898..>3679
                     /locus_tag="VFO88_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9545504.1"
                     /translation="MAVAVRTLQNFVGGSWVEATGDGVREIVSPVTGETLAEAPDASP
                     EDVSRAAAAARAAQAGWEALSVFERAEVMHRIADVLEERQDDLARSLTLENGKPFKAE
                     ALDDITEMADNFRSSAEDAKRLESAVIPTQDAGKRMITLRRANGVYAAITPWNFPSMI
                     PAELIAPGIAMGNTFVVKPSEWTPIAMADFMQAMADAGLPDGVVNLIYGGGDVGERLV
                     TDDEVDAIAFVGSHTTAEKIVRAAGLRRTLMEASGNGPVVVCA"
BASE COUNT          531 a         1342 c         1298 g          508 t
ORIGIN      
        1 gaccacgccg acgcaggtcc cggcgaagtc gagctcctcg tcgcggtaga cgcccggcat
       61 ctcggcggtc tcgcccccga ggagcgcgca gccggcctcg cggcagacgt cggccgcgcc
      121 ctcgacgagc tccgcgacct gctcgaggtc gagccggtgc gccgcgacgt agtcgaggaa
      181 gaagagcggg tcggcgccgg tcgtcaggac gtcgttcacg cagtgcgctg cgaggtcgcg
      241 cccggcgtcg cggagccgcc ccgcgcgtcg cgcgaggacg agcttcgtcc cgaccccgtc
      301 cgtcgaggcc gcgagcagac gctcgtcgtc gagggcgtag agcccggcga agccgccgag
      361 cccgccgacg accgccgccg tccgcgtcga gtccgccgcc gcgcggagcc ggtcgaccac
      421 cgactcggcc gccgcgagcg agacccctgc gtcctcgtag ctcccgctca tgcgagcgcc
      481 tcgccgacga gctcgggccg cccctccggc ccgccgtcct cgaaggagac gagcgaatcg
      541 ccgatcgtca ccgtcgggcc gtccggctcg agcccgagca cgcgcgcgag cgccgccgcc
      601 ttcccctcgg gatcgtcgga gcgcaggcgg agctcggcga cccggagctc ctcctcggag
      661 tcgagcgcgg cctcgatcca gtcgcgggga gggtgcacct ccaggcgata gccggcgtcg
      721 gtcgagatga acgtccgctt cccgggatga tcctggatct tcaacccgag gtcggacgcc
      781 cggtcgagca cctcctcgta gccttcctcg tcgagcgcga cgccgaggtg gtcgacgcgc
      841 ggcacctccc agtgcccggg ctgcaggacg acgttcacgg cgccgcgccc gagctcgacc
      901 aggcggagcc ggaaccccat ggcgtcgagc tcctcccacg acttgcccgg ctcgaaccag
      961 tgcgtgccct ccccgatcca cccgtagcgg gcgatgaggt cgaaccccag ccgccccagg
     1021 tagcgcgcct cgaccagccg gaccttcctc gtcacgaggt ggtagtggaa caggtgcacc
     1081 gcggcggccc atgctacccc ggccgggccg cggtagagga atcggcggag gggcaaagaa
     1141 ccggcccgta tgtctactca acctcccgta cctcccccgc cggcggcgcc gccgtactac
     1201 gggccgggcc cctcggggat gccgccgctc tccgcgccga acatggagtt cgtcgtctac
     1261 atcgtcgcgc tcctcgggct cgggctgatc acggcgctcg ccgagaccgt cgtctggtcg
     1321 cagttcgtat cggtcggcgg ctggatcacc gcggcgtacc tcgtctcgcg cggcttcgcg
     1381 aagatggggc gcgcctcgga caccccgtag ggcgagcggc gggcgagacg atgctccgca
     1441 aagcggctcc tttcccgagg aggcgagccg ctatgcgcag atcgagcgcc cgtcggctcg
     1501 tgcttgcgaa cgcccgggcc gggcgcgtac ggttcacgcc gtgacggtcg cgcccgatca
     1561 tctctggcgg cacttcacgc ccgcgggcgc cgcgccgatc gtgatcgacc gcgccgaggg
     1621 ctgccacatc tacgacaccg aggggaaccg ctacctcgac gcgctcgccg cgctctactg
     1681 cgtgaacatc ggctacgggc cgtacccgga gatcgccgag gccgcccggg cgcagctcga
     1741 gaggctcccg ttcttcacga actgggtcgg gttcgcgacg ccgcccgcgc tcgagctgac
     1801 cgagaagctc tccgagctcg tcccgatcga cgtcgggcgg atcttcctcg tctcgggcgg
     1861 ctcggaggcc gtcgaggcgg cgctcaaggt cgcgcgccag tatcaccggc tgcgcggcga
     1921 gccgacccgc tacaagtaca tcacccggcg cagcgcctac cacggcacga cgatgggcgc
     1981 gatctcgatc aacgggagcc cctcgctccg cgcccagttc gagccgctcc tccccggctg
     2041 cctccgcgcg ccgatgccgt accgctaccg ctgcccgtac tgctcggaca agtccgcgtg
     2101 cacgctccgc tgcgcggacg aggtcgacga catcatccgg aacgaggatc cgtccacggt
     2161 cgcggcggtg atcatggagc ccgtccagaa ctcgggcggc tcgatcgttc cgcccgaggg
     2221 ctacttccag cggatccgcg agatctgcga cgcgcacggc gtcctgctcg tggccgacga
     2281 ggtgatctgc ggcttcgggc gcgtcggcga ctggttcggg acgacccgct acgggatcga
     2341 gccggacatg atgacgatgg cgaagggcct cacctccgcg tacgcgccgc tcggcgcgct
     2401 cgtcgcgagc acgaaggtga tcgagccctt cttctccgac ccgaagacga tgctcatcca
     2461 cgggatcacg ttcggcggcc acccgctctc gtgcgcgatc gcgctcgcga acctcgaggt
     2521 cttcgagcgc gacgacctga tcgggcacgt ccgggagatg acgccggtct tccaggagga
     2581 gttcggccgg ctcgcggacg agcacccgat gatcggcgac ctgcgcggcg acggctgctt
     2641 ctactcgttc gagctcgtga aggacaagga gacgaaggcg acgttcacgc cagccgagcg
     2701 cgacgagctg atcaagacgt tcttcgtccc gcgcgcacgg gagctcggca tctacatgcg
     2761 cgtggacgac cgggccgaga cggccgcgca gttctcgccg ccgctcgtcg ccggccgcga
     2821 ggagctcgac gagttcctcg gcgtcttccg ccaggtgctc gacgaggcat acgagcggtt
     2881 cgtctagcct gagccgcatg gcggtcgcgg tccggacgct ccagaacttc gtcggcgggt
     2941 cgtgggtcga ggcgacgggc gacggcgtgc gcgagatcgt ctcgcccgtg accggcgaga
     3001 cgctcgccga ggcgccggac gcgtctcccg aggacgtgtc gcgcgcggcc gcggctgcgc
     3061 gggcggcgca ggccggctgg gaagcgctct ccgtcttcga gcgcgccgag gtcatgcacc
     3121 ggatcgccga cgtgctcgag gagcggcagg acgatctcgc ccgctcgctc acgctcgaga
     3181 acggcaagcc gttcaaggcc gaggcgctcg acgacatcac ggagatggcg gacaacttcc
     3241 gctcgtcggc ggaggacgcg aagcggctcg agagcgccgt catcccgacg caggacgccg
     3301 ggaagcggat gatcacgctc cggcgcgcga acggggtcta cgcggcgatc acgccgtgga
     3361 acttcccctc gatgatcccg gcggagctga tcgcgccggg gatcgcgatg ggcaacacgt
     3421 tcgtcgtcaa gccgtccgag tggacgccga tcgcgatggc cgacttcatg caggcgatgg
     3481 ccgacgcggg gctcccggac ggggtcgtca acctgatcta cggcggcggg gacgtcggag
     3541 agcggctcgt gacggacgac gaggtggacg cgatcgcctt cgtcggctcg cacacgacgg
     3601 cggagaagat cgtgcgggcg gcgggcctca ggcgcacgct catggaggcg agcgggaacg
     3661 gcccggtcgt cgtctgcgc
//
LOCUS       DASTFC010000270         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_6644,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000270 DASTFC010000000
VERSION     DASTFC010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6644"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFO88_08000"
     CDS             <1..718
                     /locus_tag="VFO88_08000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9545505.1"
                     /translation="RAYIWPPAIDPLAPKNMALSPEDAAYIVDQFGIDVERPLITQVS
                     RFDPWKDPLGVIEAWRIVREEHPGLQLALVGSMAHDDPEGWDYYNRTVEAAAGDPDVY
                     ILSNLNNVGSVEVNAFQVHSAAVLQKSIREGFGLTVTEALWKGRPVVAGSVGGIVDQV
                     THGETGWLVGSVEECAVAVSEVLAEPEIARAMAKAGKEHVRSRFLTPRLLRDWLALFN
                     VLDGRETDAGSALAAVGAAG"
     gene            757..2241
                     /locus_tag="VFO88_08005"
     CDS             757..2241
                     /locus_tag="VFO88_08005"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET9545506.1"
                     /translation="MATRRKLIVVSNRGPVSFGRVDGERVVRRGGGGLVTALAGLLGQ
                     HDVTWVASAMTHEDRVVADENAGSALDDVARDGSPYRLRLVAHDDEAYHRYYNVISNP
                     LLWFVQHRLWGLADGPDVDPGIWKAWREGYVTVNRAFADTVCSELEREPDAEVFFHDY
                     HLYLAPGVVRERTPAATLLQFVHIPWPEPDAWIVLPEAMRRAIYEGLLANDVVSFHTE
                     RWRRNFLAGAVEHAGAEPDFDRYDAALDGRVTAVRYSPISVDPEEFERLRSSEAVLDA
                     ERALTSSRPEVLLVRVDRTDPSKNAVRGFRAFALYLETHPEMHGRIGFLARLAPSRQD
                     IPEYVDCLAAIERAARAVNDRFARPGWSPVTLVLEDDFAGSVAAYKQFDVLLVNPVAD
                     GLNLVAKEAPLVNERDGVVILSETAGAHEELEDFVVSVNPFDVAAQAEAIHEAVTLGV
                     DARRRRLEGIRARVREHSIHAWTAQQLHDLDEAPARHDRTLRVP"
     gene            2238..2705
                     /gene="moaC"
                     /locus_tag="VFO88_08010"
     CDS             2238..2705
                     /gene="moaC"
                     /locus_tag="VFO88_08010"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET9545507.1"
                     /translation="MTDAERTPLSHVDETGEVRMVDVGGKPLSRRRARAAATVRMRPE
                     TAARLGSLPKGDALATARIAGIMAAKRTADLIPLCHPLPLTHLEVSVTVGEGRAEIEA
                     TAETTAQTGVEMEALVAASVAALALYDMAKAVDKEMVVEEVRLLEKTKEEQGA"
     gene            2702..3181
                     /locus_tag="VFO88_08015"
     CDS             2702..3181
                     /locus_tag="VFO88_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET9545508.1"
                     /translation="MNAAVLTVSDGVSHGAREDTSGDLLAELLAGEGYDVVRRVVPDD
                     RAEIAHALRELARDSRLVLTTGGTGVAPRDVTPEATRDVLDRPAPGIAEALRADSRAK
                     TPHGLLSRGVAGTIGHALVVNLPGSTGGCRDGFEVLRPALGHALALLADERTAHRQT"
     gene            3178..4038
                     /locus_tag="VFO88_08020"
     CDS             3178..4038
                     /locus_tag="VFO88_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HET9545509.1"
                     /translation="MSTVIRYPGLFASLVKVEHTVFALPFAYVGAFLAVDGVPGAADL
                     LWITVAMVGARSLAMALNRLIDAEIDARNPRTAGRELPRGALSRPEVIAFCLASLAVF
                     LVAVWQLAPLVRWLWPIPVVAFVVYPYLKRVTWLGHAWLGAVDGLAPVGAWVAITNEL
                     PWEAWALGGAVAVWVAGFDLLYALFDLEIDRAQGLRSFPERFGVPATFRAARACHVAT
                     VALLAVAGLGLPVGVLYWIGVGVVAGLLAYEHALVSPGDLRRLDAAFFTMNGVISLAF
                     FAFVLLDVTV"
     gene            4035..4619
                     /locus_tag="VFO88_08025"
     CDS             4035..4619
                     /locus_tag="VFO88_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9545510.1"
                     /translation="MIRARGLERRFGDKRVLAGLDLDVAAGGFVAVTGPNGSGKSTLL
                     ALLAGLLAPTGGELAIEARREEVGYLAHEPLVYRELSALENLELYGRLYRVPERRERI
                     GMLLERYGLWPVRHERVSAYSRGMAQRLALCRTLLHDPSLVLLDEPYSALDADGARLL
                     DAELAALTGERTLVVSTHDPDRVRPLATQEVSLA"
BASE COUNT          560 a         1712 c         1701 g          662 t
ORIGIN      
        1 gcgcgcctac atctggcccc cggcgatcga cccgctcgcg ccgaagaaca tggccctctc
       61 gcccgaggac gccgcgtaca tcgtcgacca gttcgggatc gacgtcgagc ggccgctcat
      121 cacccaggtt tcccgcttcg atccgtggaa ggacccgctc ggcgtgatcg aggcctggcg
      181 gatcgtccgc gaggagcacc cgggcctcca gctcgcgctc gtcggctcga tggcgcacga
      241 cgacccggag ggatgggact actacaaccg gacggtcgag gcggcggccg gtgacccgga
      301 cgtctacatc ctctcgaacc tgaacaacgt cggatccgtc gaggtgaacg cgttccaggt
      361 gcactcggcc gcggtgctcc agaagtcgat ccgcgagggc ttcggcctca ccgtgacgga
      421 ggcgctctgg aagggccggc cggtcgtcgc cgggagcgtc ggcgggatcg tcgaccaggt
      481 gacgcacggc gagacggggt ggctcgtcgg ctcggtcgag gagtgtgccg tggccgtgtc
      541 ggaggtcctc gcggagcccg agatcgcgcg cgcgatggcg aaggccggca aggagcacgt
      601 ccggtcgcgg ttcctgacgc cgcggctcct gcgggactgg ctcgcgctct tcaacgtgct
      661 cgacgggcgt gagacggacg cgggctcggc gctcgccgcc gtcggcgccg caggctgagt
      721 cccgggcggg ccggcccggg tagggtgacc ggcggcatgg cgacgcgccg gaagctgatc
      781 gtcgtctcga accgcgggcc cgtctccttc gggcgggtgg acggcgagcg ggtcgtgcgc
      841 cgcggcggcg gcgggctcgt gaccgcgctc gcggggctgc tggggcagca cgacgtcacg
      901 tgggtcgcga gcgcgatgac ccacgaggac cgcgtggttg ccgacgagaa cgcgggctcc
      961 gcgctcgacg acgtcgcccg cgacggctcg ccctaccggc tgcgcctcgt cgcccacgac
     1021 gacgaggcgt accaccggta ctacaacgtc atctcgaacc cgctcctctg gttcgtccag
     1081 caccggctgt gggggctcgc cgacgggccg gacgtcgacc cggggatctg gaaggcctgg
     1141 cgcgagggct acgtcaccgt gaaccgcgcg ttcgcggaca cggtctgctc cgagctcgag
     1201 cgcgagcccg acgcggaggt cttcttccac gactaccacc tgtaccttgc gcccggcgtg
     1261 gtgcgcgagc gtacgcccgc cgccacgctc ctccagttcg tccacatccc gtggccggag
     1321 cccgatgcgt ggatcgtcct cccggaggcg atgcgccgcg cgatctacga ggggctcctc
     1381 gcgaacgacg tcgtctcgtt ccacacggag cgctggcggc ggaacttcct cgcgggggcc
     1441 gtcgagcacg cgggcgcgga gcccgacttc gaccgctacg acgccgcgct cgacgggcgc
     1501 gtcaccgcgg tgcgctactc gccgatctcc gtcgacccgg aggagttcga gaggctcagg
     1561 tcgagcgagg ccgtgctcga cgccgagcgt gcgctcacgt cgtcgcggcc ggaggtgctc
     1621 ctcgtgcgcg tcgaccgcac cgacccgtcg aagaacgcgg tccgcggctt ccgcgccttc
     1681 gcgctctatc tcgagacgca cccggagatg cacgggcgga tcggcttcct cgcccggctc
     1741 gcgccgtcgc ggcaggacat cccggagtac gtcgactgcc tggccgcgat cgagcgcgcc
     1801 gcgcgcgccg tgaacgaccg cttcgcccgg cccggctggt cgcccgtgac gctcgtgctc
     1861 gaggacgact tcgccggctc ggtcgccgcc tacaagcagt tcgacgtcct gctcgtgaac
     1921 ccggtcgcgg acggcctgaa cctcgtggcg aaggaggcgc cgctcgtgaa cgagcgggac
     1981 ggcgtcgtca tcctctcgga gacggccggc gcgcacgagg agctcgaaga cttcgtcgtc
     2041 agcgtgaacc cgttcgacgt ggccgcgcag gccgaggcga tccacgaggc ggtgacgctc
     2101 ggcgtcgacg cgcgccggcg gcggctcgag ggcatccggg cccgcgtgcg cgagcactcg
     2161 atccacgcct ggacggccca gcagctccac gacctcgacg aggccccggc gcggcacgac
     2221 cgtacacttc gcgttccgtg accgacgccg agcgaacgcc gctctcccac gtcgacgaga
     2281 cgggggaggt gcgcatggtg gacgtgggcg gcaagccgct gtcgcgccgt cgtgcgcggg
     2341 ccgcggcgac ggtgcggatg cggccggaga cggcggcccg gctcggctcg ctcccgaagg
     2401 gcgacgcgct cgcgaccgcg cggatcgcgg ggatcatggc cgccaagcga acggccgacc
     2461 tcatcccgct ctgccatccg ctcccgctca cgcacctcga ggtctcggtg acggtcggcg
     2521 agggccgggc cgagatcgag gcgaccgcgg agacgaccgc gcaaacgggc gtcgagatgg
     2581 aggcgctcgt cgccgcctcc gtcgccgcgc tcgcgctcta cgacatggcg aaggccgtgg
     2641 acaaggagat ggtcgtcgag gaggtccggc tcctcgagaa gacgaaggag gagcagggcg
     2701 catgaacgcg gccgtgctca cggtctcgga cggcgtctcg cacggcgcgc gggaggacac
     2761 gagcggcgac ctcctcgccg agctcctcgc gggtgagggc tacgacgtcg tgcgccgcgt
     2821 cgtccccgac gaccgcgccg agatcgccca cgcgttgagg gagctcgcgc gcgactcccg
     2881 gctcgtcctc acgaccggcg ggaccggcgt cgcgccgcgc gacgtgaccc cggaggcgac
     2941 gcgggacgtc ctcgaccggc ccgcgccggg aatcgccgag gcgctgcgcg ccgactcgcg
     3001 ggcgaagacg ccgcacgggc tcctctcccg cggcgtcgcc gggacgatcg ggcacgcgct
     3061 cgtcgtcaac ctgcccggct cgacgggcgg gtgccgcgac ggcttcgagg tgctccgccc
     3121 cgcgctcggc cacgcgctcg cgctcctcgc ggacgagcgg acggcgcacc ggcagacgtg
     3181 agcacggtca ttcgctaccc ggggctcttc gcgtcgctcg tgaaggtcga gcacaccgtc
     3241 ttcgcgctcc cgttcgccta tgtcggcgcc ttcctcgcgg tcgacggcgt cccgggcgcg
     3301 gccgacctcc tctggatcac cgtggcgatg gtcggcgccc gctcgctcgc gatggcgctc
     3361 aaccggctga tcgacgcgga gatcgacgcg cggaacccgc gcaccgccgg gcgcgagctg
     3421 ccgcgcggag ccctctcccg gccggaggtg atcgcgttct gccttgcgtc gctcgcggtc
     3481 ttcctcgtcg ccgtctggca gctcgcgccg ctcgttcgct ggctctggcc gatcccggtc
     3541 gtcgcgttcg tcgtctaccc gtacctgaag cgcgtgacgt ggctcggcca cgcctggctc
     3601 ggcgcggtcg acgggctcgc tccggtcggc gcgtgggtcg cgatcacgaa cgagcttccc
     3661 tgggaggcgt gggcgctcgg cggcgccgtc gcggtctggg tcgccgggtt cgacctcctc
     3721 tacgcgctct tcgacctcga gatcgaccgc gcgcaggggc tccgctcgtt cccggagcgc
     3781 ttcggcgttc cggcgacctt ccgggcggcc cgtgcgtgcc acgtcgccac ggtcgcgctc
     3841 ctcgcggtcg ccgggctggg gctgccggtc ggggtgctct actggatcgg cgtgggcgtc
     3901 gtcgcggggc tcctcgcgta cgagcacgcg ctcgtctcgc ccggcgacct gcggcggctc
     3961 gacgccgcgt tcttcacgat gaacggcgtc atctcgctcg cgttcttcgc cttcgtcctc
     4021 ctggacgtga cggtgtgatc cgcgcgcggg gcctcgagcg gcgcttcggg gacaagcgcg
     4081 tgctcgccgg gctcgacctc gacgtcgccg ccggcgggtt cgtcgccgtg accgggccga
     4141 acgggtcggg gaagtcgacg ctgctcgcgc tcctcgcggg gctcctcgcg ccgaccggcg
     4201 gcgagctcgc gatcgaggcc cggcgcgagg aggtcggcta cctcgcgcac gagccgctcg
     4261 tctaccggga gctctcggcg ctcgagaacc tcgagctcta cgggcgcctt taccgcgtgc
     4321 ccgagcggcg cgagcggatc gggatgctgc tcgagcgcta cggcctctgg cccgtgcgcc
     4381 acgagcgcgt ctccgcgtat tcgcggggca tggcgcagcg gctcgcgctc tgccggacgc
     4441 tcctgcacga tccgagcctc gtcctcctcg acgagccgta ctccgcgctc gacgcggacg
     4501 gggcgcggct cctcgacgcc gagctcgccg cgctcacggg cgagcggacg ctcgtcgtct
     4561 cgacccacga ccccgaccgc gtgcggccgc tcgcgacgca ggaggtctcg ctcgcgtgag
     4621 cggctacctc ggcga
//
LOCUS       DASTFC010000271         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1407319,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000271 DASTFC010000000
VERSION     DASTFC010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407319"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="obgE"
                     /locus_tag="VFO88_08030"
     CDS             complement(<1..1083)
                     /gene="obgE"
                     /locus_tag="VFO88_08030"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET9545511.1"
                     /translation="MFQDRARIDVEAGRGGDGSLSFRREKYVPKGGPDGGDGGKGGDV
                     LLVADPDLRDLGFLARKQRVKAARGRHGQGANKKGADGEDVELRVPVGTQVFDEEGRL
                     VADLAHAGARTVLARGGLGGYGNKRFASPTEQAPRVAEVGQAGEEGTFELRLKLLADA
                     ALLGFPNAGKSSLLRRISNAKPKVAEYPFTTLAPVLGTVEAPDGRQLTVADVPGLVEG
                     ASEGVGLGDEFLAHLERARLFLHVIDVTEDAAERFTAIDRELAAYGAGLAERPQLVVL
                     NKIDLVPEPPPFPLADPRVVGVVPVSAATGAGIDALRRALFEVAAAAAPPDGAPAGGP
                     EEDELADFLVYRPQPPRARPFRIFRGD"
     gene            complement(1102..1347)
                     /gene="rpmA"
                     /locus_tag="VFO88_08035"
     CDS             complement(1102..1347)
                     /gene="rpmA"
                     /locus_tag="VFO88_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET9545512.1"
                     /translation="MAHKKGLGSSKNGRDSESKRLGVKMFDGQEIKAGMIVVRQRGTR
                     FRPGAGTGLGRDHTIFATRDGRVEFSASGATRTISVV"
     gene            complement(1347..1772)
                     /gene="rplU"
                     /locus_tag="VFO88_08040"
     CDS             complement(1347..1772)
                     /gene="rplU"
                     /locus_tag="VFO88_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET9545513.1"
                     /translation="MTYAIIGIGGKQYRVREGEWLLVDRVATEEGKTFKPDVLLLGGD
                     GEPKLGDDLKGAEVTAVVTGHVLGEKIVVGKHRQRTGYRRRKGHRSRLSRIEIQSIGA
                     KSARKAPAKKAEGEKKETKAKSSTAAKKPAAKKKTEGKS"
     gene            complement(1812..>4005)
                     /locus_tag="VFO88_08045"
     CDS             complement(1812..>4005)
                     /locus_tag="VFO88_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET9545514.1"
                     /translation="RRSIAGNVYKGVVDNVLPGMEASFVEIGLPKNGFLYVDEIVVPE
                     LEGKRHGRRIQDLIQRGEEVLVQAVKDPMGTKGARLTTEISLPGRFLVYTPFGEGIGI
                     SRRLDDAERERLKKISKGLDLPEGGIIIRTAAEGASEEELQGDLALLQKLWATIKGRA
                     DRTKAPALVYQEAELPLRIVRDLFIRDFARVVVDHDRTYRRIVGYLKRTSQDLAQRVE
                     LYQGRERLMDVAGVDAAVRSTLDRRVNLPSGGYLIFDYAEAFTVIDVNTGRFVGARGR
                     SSGARLEDTITKNNLEAVEEVVRQLRLRDIGGIIVIDFIDMANPKNRAQVEDALRAEL
                     EKDRTKTYVVEISPLGLVEMTRQNVTDGPREILTNACPTCAGDGIVVSMETHAVDVER
                     ELRRLVRTSRHQGFRVEVAADVSPLLVGPGAARLVELERETGRRFFLETREDVHGDHF
                     VVLDRGPVSKLEPKGLPVAAGDEVDVKIVEPHAALAGEAIGRLNGYTVSVADARSLVG
                     KTVKVRIERATPGLAYAVLADAASSEAPVEAGDELEREDAALPQKAAPATKKSRRGSR
                     GGRGRKKAAAEPKPEAAEPAAQPVAEPAPEPEPEVREAEPEGEQPAVEKPKKKTRRGS
                     RGGRGRKKKTAAPTAASANGDGAAPAAEAAHEPEPGPEPEPEAASTNGEAPSEEQPKK
                     KKTRRGSRGGRGRKKKTATSAEGDAAPATDGAPEPIAAIAADEEPRLD"
BASE COUNT          446 a         1544 c         1393 g          622 t
ORIGIN      
        1 gtcgccgcgg aagatccgga acggacgggc gcgcggcggc tgcgggcggt agacgaggaa
       61 gtccgcgagc tcgtcctcct cgggcccgcc ggccggcgcg ccgtcgggcg gcgcggccgc
      121 cgcggccacc tcgaacagtg cccgccggag cgcgtcgatc ccggcaccgg tcgcggccga
      181 caccgggacg actcccacga cacgcggatc cgcgagcgga aacggcggag gctccggcac
      241 gagatcgatc ttgttcagga cgacgagctg cggccgctcg gcgagcccgg cgccgtacgc
      301 cgcgagctcg cggtcgatcg cggtgaaccg ctccgcggcg tcctcggtca cgtcgatcac
      361 gtgcaggaag agccgcgccc gctcgaggtg ggcgaggaat tcgtcgccga gcccgacacc
      421 ctcgctcgcc ccctcgacga gcccgggcac gtccgcgacg gtgagctgcc gcccgtccgg
      481 cgcctcgacc gtcccgagca ccggcgcgag cgtcgtgaag ggatactcgg ccaccttcgg
      541 cttcgcgttc gagatccgcc gcaggaggga cgactttccc gcgttcggga agccgaggag
      601 cgcggcgtcg gcgaggagct tgaggcgcag ctcgaacgtc ccctcctccc cggcctggcc
      661 gacctccgcc acgcgcgggg cctgctcggt cggcgacgcg aaccgcttgt tcccgtagcc
      721 gccgaggccc ccgcgcgcga gcacggtccg cgcaccggcg tgcgcgaggt cggcgacgag
      781 ccggccctcc tcgtcgaaga cctgcgtccc gaccggcacg cggagctcga cgtcctcgcc
      841 gtccgctccc ttcttgttcg cgccctgacc gtgccgcccg cgcgccgcct tcacgcgctg
      901 cttgcgcgcg aggaagccga ggtcgcgaag gtccgggtcg gcgacgagca ggacgtcgcc
      961 gcccttcccg ccgtccccgc cgtccgggcc ccccttcggg acgtacttct cgcgccggaa
     1021 cgagagcgag ccgtcgccgc cccgcccggc ctcgacgtct atccgggccc ggtcctggaa
     1081 caccgggtcg cccccgggcc tctaaacgac ggagatcgtc cgggtcgcgc cggaggcgct
     1141 gaactcgacg cggccgtcac gcgtggcgaa gatcgtgtgg tcgcggccga gccccgtccc
     1201 cgcacccggg cggaagcgcg tcccgcgctg gcggacgacg atcatgccgg ccttgatctc
     1261 ctggccgtcg aacatcttga cgccgagccg cttggactcg gagtcgcgcc cgttcttcga
     1321 ggagccgagc cccttcttgt gtgccactag ctcttccctt ccgtcttctt cttcgccgcc
     1381 ggcttcttcg ccgccgtcga ggacttcgcc ttcgtctcct tcttctcccc ctcggccttc
     1441 ttcgcaggcg ccttgcgggc gctcttcgcg ccgatcgact ggatctcgat ccgcgagagg
     1501 cggctccggt ggcccttgcg ccggcggtac ccggtgcgct gccggtgctt gcccacgacg
     1561 atcttctcgc caagcacgtg gccggtcacg accgcggtca cctcggcgcc cttcaggtcg
     1621 tcgccgagct tcggctcgcc gtcgccgccg aggagcagga cgtccggctt gaacgtcttg
     1681 ccctcctccg tggcgacccg gtccacgagg agccactcgc cctcgcggac gcggtactgc
     1741 ttgccgccga tgccgatgat cgcgtaggtc atgtcgtctc cgtaaggaag cgggcttccg
     1801 cccgctcggg cctagtctag ccgaggctcc tcgtcggcgg caatcgccgc gatcggctcc
     1861 ggcgcgccgt ccgtcgcggg cgccgcgtcg ccctccgcgg acgtcgcggt cttcttcttc
     1921 cggccgcgcc cgccgcgcga gccgcgccgc gtcttcttct tcttcggctg ctcctcggac
     1981 ggcgcctcgc cgttcgtgga ggccgcctcg ggctcgggct ccggcccggg ctccggctcg
     2041 tgggccgcct cggcggccgg cgcggcgccg tctccgttcg cgctcgcggc cgtcggcgcg
     2101 gccgtcttct tcttgcggcc gcgcccgccg cgcgagccgc gccgcgtctt cttcttcggc
     2161 ttctccaccg ccggctgctc gccctcgggc tccgcctcgc gtacctccgg ctcgggctcg
     2221 ggcgcgggct ccgcgaccgg ctgggcggcc ggctcggcgg cctcgggctt cggctcggcg
     2281 gccgccttct tgcggccgcg cccgccgcgc gagccgcggc gcgacttctt cgtcgcgggt
     2341 gccgccttct ggggcagcgc ggcgtcctcg cgctccagct cgtcgcccgc ctcgaccggc
     2401 gcctccgacg aggcggcgtc ggccaggacc gcgtaggcca ggcccggagt cgcgcgctcg
     2461 atccggacct tgaccgtctt gccgacgagc gagcgcgcgt cggcgaccga gaccgtgtag
     2521 ccgttcagcc gcccgatcgc ctcgcccgcg agcgcggcgt gcggctccac gatcttcacg
     2581 tcgacctcgt cgcccgcggc gaccgggagc cccttcggct cgagcttcga gaccgggccg
     2641 cggtcgagga cgacgaagtg gtcgccgtgc acgtcctccc gcgtctcgag gaagaagcgc
     2701 cggccggtct cgcgctcgag ctccacgagc cgcgccgcgc ccggcccgac gaggagcggc
     2761 gagacgtccg ccgccacctc gacccggaac ccctggtggc gcgacgtccg cacgagccgc
     2821 cgcagctcgc gctcgacgtc gaccgcatgg gtctccatcg agacgacgat cccgtcgccc
     2881 gcgcacgtcg ggcaggcgtt cgtgaggatt tcgcgcggcc cgtccgtcac gttctggcgc
     2941 gtcatctcga cgagcccgag cggggagatc tcgacgacgt acgtcttcgt ccggtccttc
     3001 tcgagctcgg cgcgcagcgc gtcctcgacc tgcgcccggt tcttcgggtt cgccatgtcg
     3061 atgaagtcga tgacgatgat cccgccgatg tcgcgcaggc ggagctggcg gacgacctcc
     3121 tcgaccgcct cgaggttgtt cttcgtgatc gtgtcctcga gccgcgcgcc gctcgagcgg
     3181 ccgcgcgcgc cgacgaagcg gccggtgttc acgtcgatca cggtgaacgc ctccgcgtag
     3241 tcgaagatga ggtagccgcc ggacgggagg ttcacccgcc ggtcgagcgt cgagcggacg
     3301 gccgcgtcga cgccggccac gtccatgagc cgctcgcggc cctggtagag ctcgacgcgc
     3361 tgcgcgagat cctgcgacgt ccgcttgagg tagccgacga ttcgccggta cgtccggtcg
     3421 tggtcgacga cgacgcgcgc gaagtcgcgg atgaagaggt cgcggacgat cctgagcggc
     3481 agctcggcct cctggtagac gagcgccggc gccttcgtcc ggtcggcgcg gcccttgatc
     3541 gtcgcccaga gcttctggag gagcgcgagg tcgccctgga gctcctcctc ggaggcgccc
     3601 tccgcggccg tccggatgat gatcccgccc tcgggcaggt cgaggccctt ggagatcttc
     3661 ttcagccgct cacgctcggc gtcgtcgagc cggcgcgaga tgccgatccc ctcgccgaac
     3721 ggcgtgtaga cgaggaagcg gccgggcagc gagatctccg tcgtgagccg cgcgcccttg
     3781 gtgcccatgg ggtccttgac cgcctggacg aggacctcct cgccgcgctg gatcagatcc
     3841 tggatgcggc ggccgtgccg cttcccctcg agctcgggga cgacgatctc gtccacgtag
     3901 aggaagccgt tcttggggag gccgatctcg acgaacgacg cctccatgcc ggggaggacg
     3961 ttgtcgacga cgcccttgta gacgttgccg gcgatcgacc ggcgg
//
LOCUS       DASTFC010000272         6048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1424010,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000272 DASTFC010000000
VERSION     DASTFC010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424010"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFO88_08050"
     CDS             complement(<1..736)
                     /locus_tag="VFO88_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET9545515.1"
                     /translation="MARTTVRTDELDYDLPPELVAQRPPARRDESRLLVHDRATGAVR
                     HRRFRDLPEEAGDALVVVNDTRVVPARLRLRRPGGGQAEVLLLEPLGDGLWEGLARPT
                     KRLRPGMRLGPVELVEHVGEGRWLLRLEGEPAGEPPLPPYITEPLADAERYQTVYARE
                     TGSAAAPTAGLHFTPELLARLDVEHVTLHVGLDTFRPVAAATLDAHELHSERYEVAAE
                     AWERIAAAERVVAVGTTTTRVLETLAR"
     gene            755..1228
                     /locus_tag="VFO88_08055"
     CDS             755..1228
                     /locus_tag="VFO88_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545516.1"
                     /translation="MKPESIEWARRRLAEAGADRDPAALDATLERTREALETFAARTA
                     ELEASVPERLDAAIRDGIQAEARPIGRNLAELRGLANGTVRRLERLQADLDAERRARV
                     EDLSLLVDLIASGWRGVEGRLDRIERMFDRLERSLQERPLAELYDLEERQRRSGA"
     gene            complement(1238..2623)
                     /locus_tag="VFO88_08060"
     CDS             complement(1238..2623)
                     /locus_tag="VFO88_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9545517.1"
                     /translation="MRSLRFRLPVLFLVAIVVASAVAAVIAVGLFQNVARQDSLRELR
                     RQASGLADLYANQALAASEEGRRAPRFAAARLERATGTRLYYAGLNPFPGDDSGLRTL
                     ELSVLPDQEAFRAGEPQTFEFRPPGQDRVYLAAAHPLNIGDQTFGALVAAKPRAELQE
                     RWQPLVERLALALLAGLFVAAGLGWYLSRRLTAPVVALTRATDEVARGRYDVVLPRTR
                     KRDEIGRLTERFRDMTRRLAEAEERERNFLMSVSHELRTPLTAIRGHVDALAEGLVED
                     AESREASLDVIRTESDRLARLVGDLLDLARLDAHRFRLAQDEVELCRVVENAFQARTE
                     EAKRRSIEYEQRLGADPVLHTDGDRVLQIVTNLIDNAFRWTPDGGRISVELEAANGTI
                     SVAVADSGPGVRPEDRERIFRPFYTGQGSQGTGLGLAIARELAHALGGDLRLDSANGS
                     GSRFELTLPAR"
     gene            complement(2633..3349)
                     /locus_tag="VFO88_08065"
     CDS             complement(2633..3349)
                     /locus_tag="VFO88_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9545518.1"
                     /translation="MADSGRQRILVVEDETNIASFVAMYLQKAGFEVGVASTGHEALA
                     KAGSDSPALIVLDLMLPDLDGIDVCRRVRMGSDVPILMLTARDDDVDKIIGLEVGADD
                     YLTKPFNPRELVARVKSILRRSTTRRTLPEGGTLEQGPLRIDSGRREVTVAGKEVQLA
                     PKEFDLLWELLDHRGLVLTRDQLLERVWGYTFAGDTRTVDVHVGQLRRKLGDACPIVT
                     VWGVGYKVATEPKAAQASRA"
     gene            complement(3383..3856)
                     /locus_tag="VFO88_08070"
     CDS             complement(3383..3856)
                     /locus_tag="VFO88_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545519.1"
                     /translation="MRLKVSPLVLAALCALGLTPGAGAQPLERELAGNGSQLSLTNGR
                     GTAAVHSRDGSLLGTVGRGRIVVVNRLAGGKTTVRFWGCEERRRPAPRTVVCIGRGLR
                     VSVVNGSWITTFKGRGVNASAVVEGRLRLRGQRGSFEIDGVTRSWPSTFRTYMLG"
     gene            3938..4549
                     /gene="pdxH"
                     /locus_tag="VFO88_08075"
     CDS             3938..4549
                     /gene="pdxH"
                     /locus_tag="VFO88_08075"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET9545520.1"
                     /translation="MPDFEPLREEDVAADPVAQFRTWFREAEEAGVRLPETMILATAS
                     ADGAPSARAVLLKGVDERGFVFFTNRESRKGRELAANPRAALVFHWPALGRQVRVEGR
                     ASAVDDAEAEAYFRTRPRGSRIGAWASPQSSVLASREELGRRVAEAEAAHPGDDVPLP
                     PFWGGYRVEPEAIELWQHRESRLHDRLRYRREGDGWVLERLAP"
     gene            complement(4553..5413)
                     /locus_tag="VFO88_08080"
     CDS             complement(4553..5413)
                     /locus_tag="VFO88_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET9545521.1"
                     /translation="MAGEITRVGVVGLGTMGAGIAQVCAQAGFETVGVEVTDELGAKA
                     RERIGYYLGRSVEKERITAEERDAALARLTTTTARADLARCDLVIEAIVEDLGAKREL
                     VRELDGILEPDAIVATNTSALSVTEIARASAHPERVVGMHFFNPAPVLPLVEVVRTEL
                     AAEEPYERAYAFAAALGKEPVRCSDTPGFIVNRILIPLLNDCVRVLDEAGVSPEDLDR
                     AMRFGAGWPIGPCALVDLIGVDIHVHASEALHAALGEARMAPPERLVRMQAEGKLGRK
                     TGEGFFTYES"
     gene            5482..>6048
                     /locus_tag="VFO88_08085"
     CDS             5482..>6048
                     /locus_tag="VFO88_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9545522.1"
                     /translation="MAATVAERKLLVDGEWIETGEWQEVRSPYSGDVVGRHPVADAAL
                     ARRAVEAAARALAEPLPAHRRAAVLDAVAHLLQERQEDLAQTICAEAGKPLKAARVEA
                     SRAVSTYTTAATVARTLAGEVVPMDASEAGTGKLAFTIREPIGIVGAISPFNFPANLV
                     AHKIAPALAAGCPVVLKPASQTPLSALFL"
BASE COUNT          774 a         2229 c         2270 g          775 t
ORIGIN      
        1 cgcgcgcgag cgtctcgagc acgcgggtcg tcgtcgtccc gacggcgacg acccgctcgg
       61 cggccgcgat ccgctcccac gcctcggccg cgacctcgta gcgctcgctg tggagctcgt
      121 gcgcgtcgag cgtggccgcc gcgacgggcc ggaaggtgtc gaggccgacg tgcagggtca
      181 cgtgctcgac gtcgagccgc gcgagcagct cgggcgtgaa gtggagcccg gcggtcggag
      241 cggcggccga gccggtctcg cgcgcgtaga ccgtctggta gcgctcggcg tccgcgagcg
      301 gctcggtgat gtacggcgga agcggcggct cgccggccgg ctcgccctcg agccgcagga
      361 gccagcgccc ctcccccacg tgctcgacga gctcgacggg gccgagtcgc atccccggcc
      421 gcaggcgctt cgtcgggcgc gcgagcccct cccacagccc gtcgccgagc ggctcgagga
      481 ggagcacctc cgcctggcct ccgcccggcc ggcggagccg caggcgcgcg gggacgacgc
      541 gcgtgtcgtt cacgacgacg agcgcgtcgc ccgcctcctc cggcaggtcg cggaaccgtc
      601 ggtgccggac ggccccggtc gcccggtcgt gcacgaggag acggctctcg tcgcgccgcg
      661 cgggaggccg ctgcgcgacg agctctggag gcaggtcgta gtcgagctcg tcggttcgca
      721 cggtcgtccg ggccacacgg taccgtggcg cctcgtgaag cccgagtcga tcgagtgggc
      781 ccggaggcgg ctcgccgagg ccggcgccga ccgcgacccg gccgcgctcg acgcgacgct
      841 cgagcgcacg cgggaggcgc tcgagacctt cgctgcccgc acggccgagc tcgaggcgag
      901 cgtcccggag cggctcgacg ccgcgatccg cgacggcatc caggcggagg cgcggccgat
      961 cggccggaac ctcgccgagc tccgcggtct cgcgaacggg accgtccgcc ggctcgagcg
     1021 gctccaggcc gacctcgatg ccgagcggcg cgcgcgcgtg gaggacctgt cgctcctggt
     1081 cgacctgatc gcgtcggggt ggcgcggcgt cgaggggcgg ctcgaccgga tcgagcgcat
     1141 gttcgaccgg ctcgagcgct cgctccagga gcgcccgctg gccgagctct acgacctcga
     1201 agagcgtcag cgcagaagcg gcgcgtagcg cgaccggcta ccgcgccggg agcgtcagct
     1261 cgaagcgcga cccgctcccg ttcgccgagt cgagccgcag gtcgccgccg agcgcgtgcg
     1321 cgagctcccg cgcgatcgcg aggccgagcc ccgtcccctg cgagccctgc ccggtgtaga
     1381 acggccggaa gatccgctcc cggtcctcgg gcctgacgcc cggcccgctg tcggcgaccg
     1441 ccaccgagat cgttccgttc gcggcctcga gctcgacgga gatccggccg ccgtcgggcg
     1501 tccagcggaa cgcgttgtcg atcaggttcg tgacgatctg gaggacgcgg tcgccgtccg
     1561 tgtggaggac cgggtcggca ccgagccgct gctcgtactc gatgctccgc cgcttcgcct
     1621 cctcggtgcg cgcctggaag gcgttctcga ccacgcggca gagctccacc tcgtcctgcg
     1681 cgaggcggaa ccggtgcgcg tcgaggcgcg cgaggtcgag caggtcgccg acgagccggg
     1741 cgagccggtc gctctccgtc cggatcacgt cgagagacgc ctcgcgcgac tccgcgtcct
     1801 ccacgagccc ctcggcgagc gcgtccacgt gaccgcggat cgcggtgagc ggcgttcgga
     1861 gctcgtgcga gacgctcatg aggaagttcc gctcgcgctc ctccgcctcg gcgagccggc
     1921 gggtcatgtc gcggaagcgc tcggtcaggc ggccgatctc gtcccgcttg cgcgtccgcg
     1981 ggagcacgac gtcgtagcgg ccgcgcgcga cctcgtccgt ggcgcgcgtg agtgccacca
     2041 cgggcgcggt caggcggcgc gagagatacc agccgagccc ggcggcgacg aacagcccgg
     2101 cgaggagcgc gagcgcgagc cgctcgacga gcggctgcca tcgctcctgg agctccgcgc
     2161 gcggcttggc cgcgaccagc gcgccgaagg tctggtcgcc gatgttcagc gggtgggcgg
     2221 cggcgaggta gacccggtcc tggcccggcg ggcggaactc gaacgtctgc ggctcgccgg
     2281 cgcgaaacgc ctcctggtcg ggaaggacgg acagctcgag cgtccgcagg ccggagtcgt
     2341 cgccggggaa cgggttgagg ccggcgtagt agagccgcgt gcccgtcgcg cgctcgagcc
     2401 gggcggccgc gaagcgcggg gcacggcgtc cctcctccga ggccgcgagc gcctggttcg
     2461 cgtagaggtc ggcgagcccc gacgcctggc gccggagctc ccgcagcgag tcctgccgcg
     2521 cgacgttctg gaagagcccg accgcgatca ccgccgcgac cgcggaggcg acgacgatcg
     2581 cgacgaggaa gaggacggga agccggaagc ggagcgaccg cacggcgacc gcctaggccc
     2641 ggctcgcctg cgccgccttc ggctcggtcg cgaccttgta gccgaccccc cagaccgtga
     2701 cgatcgggca ggcgtccccg agcttgcggc ggagctggcc gacgtggacg tccacggtgc
     2761 gcgtgtcgcc cgcgaacgtg tagccccaga cgcgctcgag cagctggtcg cgggtgagca
     2821 cgagcccgcg gtggtcgagg agctcccaga ggaggtcgaa ctccttcggc gcgagctgca
     2881 cctccttgcc ggccaccgtc acctcgcgcc gcccggagtc gatccggagc ggcccctgct
     2941 cgagcgtccc gccctccggg agcgtgcgcc gggtcgtcga gcggcgcagg atcgacttca
     3001 cgcgcgcgac gagctcgcgc gggttgaacg gcttcgtgag gtagtcgtcc gcgccgacct
     3061 cgagcccgat gatcttgtcc acgtcgtcgt cgcgcgccgt gagcatgagg atcgggacgt
     3121 cggagcccat ccgcacgcgc cggcagacgt cgatcccgtc gaggtcgggc agcatgaggt
     3181 cgaggacgat gagcgccggg gagtcgctgc cggccttcgc gagcgcctcg tgcccggtcg
     3241 acgcgacgcc gacctcgaac ccggccttct gcaggtacat ggcgacgaac gaggcgatgt
     3301 tcgtctcgtc ctcgacgacg aggatgcgct ggcggccgga gtcagccatg cctgcgtcgc
     3361 atcgtgccag atgccgcggc gatcaaccga gcatgtacgt ccggaacgtg ctgggccagg
     3421 agcgcgtgac cccgtcgatc tcgaacgacc cgcgctggcc gcgcaggcgc agccggccct
     3481 cgacgacggc gctcgcgttg acgccgcggc ccttgaaggt cgtgatccac gatccgttca
     3541 cgacggagac gcgcagcccc cggccgatgc aaacaacggt gcgcggagcc ggccggcgcc
     3601 gctcctcgca gccccagaag cgcacggtcg tcttcccgcc cgccaatcgg ttcacgacga
     3661 cgatgcgccc gcggccgacc gtcccgagga gcgagccgtc ccggctgtgc accgccgcgg
     3721 ttccgcggcc gttcgtgagc gagagctggc tgccgttccc cgcgagctcg cgctcgagcg
     3781 gctgggcgcc tgcgcccggg gtgagcccga gtgcgcagag cgctgcgagg acgaggggtg
     3841 aaaccttcag ccgcatttcc ggtcgtagtg tcctccgctc ggttcgcccg cgcatgatcg
     3901 cgctgtaacg attcggtaaa gcccgtaggg tgccgccgtg cccgacttcg agcccctgcg
     3961 cgaggaggac gtcgccgccg atccagtcgc gcagttccgg acgtggttcc gcgaggcaga
     4021 ggaggccggc gtgcggctgc cggagacgat gatcctcgcg accgcctcgg ccgacggcgc
     4081 tccctcggcg cgggcggtgc tgctgaaggg cgtcgacgag cgcggcttcg tgttcttcac
     4141 gaaccgggag agccggaagg gccgcgagct cgccgcgaac ccgcgggcgg cgctcgtctt
     4201 ccattggccc gcactcgggc ggcaggtgcg cgtcgagggc cgcgcctcgg ccgtggacga
     4261 cgccgaggcg gaggcgtact tccgcacacg gccgcgcggg tcgcggatcg gcgcgtgggc
     4321 ctcgccgcag agctcggtcc tcgcgtcgcg cgaggagctc gggcggcgtg tcgcggaggc
     4381 ggaggcggcc cacccgggcg acgacgtgcc gctcccgccc ttctggggcg gctaccgggt
     4441 cgagcccgag gcgatcgagc tctggcagca ccgcgagagc cgcctccacg accggctccg
     4501 ctaccgccgc gagggcgacg gctgggtgct cgagcggctc gcgccgtagc ctctagctct
     4561 cgtacgtgaa gaagccctcg cccgtcttgc ggccgagctt cccctcggcc tgcatgcgga
     4621 cgagccgctc cggcggggcc atgcgcgcct cgccgagcgc ggcgtgcagc gcctcggacg
     4681 cgtggacgtg gatgtcgacg ccgatcaggt cgacgagcgc gcaggggccg atcggccacc
     4741 cggcgccgaa gcgcatcgcc ctgtcgaggt cctccggcga gaccccggcc tcgtcgagga
     4801 cgcgcacgca gtcgttcagg agcgggatga ggatccggtt cacgatgaag ccgggcgtgt
     4861 ccgagcagcg gaccggctcc ttgccgagcg cggcggcgaa cgcgtaggcg cgctcgtacg
     4921 gctcctctgc ggcgagctcg gtgcggacga cctcgacgag cggcagcacg ggggccgggt
     4981 tgaagaagtg catcccgacc acgcgctccg ggtgcgcgct cgcgcgggcg atctccgtga
     5041 ccgagagcgc ggacgtgttc gttgcgacga tcgcgtctgg ctcgaggatg ccgtcgagct
     5101 cgcggacgag ctcgcgcttc gcgccgagat cctcgacgat cgcctcgatc acgaggtcgc
     5161 agcgggccag gtcggcgcgg gcggtcgtcg tcgtgagccg ggcgagcgcc gcgtcgcgct
     5221 cctcggccgt gatccgctcc ttctcgacgg agcgcccaag gtagtagccg atccgctcgc
     5281 gggccttcgc gccgagctcg tcggtcacct cgacgccgac ggtctcgaac ccggcctggg
     5341 cgcacacctg cgcgatcccg gcgcccatcg tcccgagccc gacgacgccg acccgtgtga
     5401 tctccccggc cacggggcgg gagcctaacg gtcgagccgc gcccgtcacg gcccttcgag
     5461 cccgcgtggt tcaatagcgg catggctgcg acggtcgcgg agcggaagct cctcgtggac
     5521 ggcgagtgga tcgagacggg cgagtggcag gaggtgcgct cgccgtactc cggcgacgtc
     5581 gtcggccgcc acccggtcgc ggacgccgcg ctcgcacgca gggccgtcga ggccgccgcc
     5641 cgggcgctcg cggagccgct cccggcgcac cggcgcgccg ccgtcctgga cgcggtcgcg
     5701 cacctgctcc aggagcgcca ggaggacctc gcccagacga tctgcgcgga ggccgggaag
     5761 ccgctcaagg cggcccgggt cgaggcctcg cgcgcggtct cgacgtacac aacggccgcg
     5821 acggtcgccc gcacgctcgc cggcgaggtc gtcccgatgg acgcgtccga ggccggcacg
     5881 gggaagctcg cgttcacgat tcgcgaaccg atcgggatcg tcggcgcgat cagcccgttc
     5941 aacttccccg cgaacctcgt cgcgcacaag atcgccccgg cgctcgcggc cggctgcccg
     6001 gtcgtcttga agccggcgag ccagacgccg ctctccgcgc tcttcctc
//
LOCUS       DASTFC010000273         7901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1259090,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000273 DASTFC010000000
VERSION     DASTFC010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259090"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFO88_08090"
     CDS             complement(<1..368)
                     /locus_tag="VFO88_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9545523.1"
                     /translation="MATTPDRRETDSGIEVKPVYTDEDVAGLELEAPGEFPFTRGPYR
                     DMYRGRPWTIRQYAGFASAEETNARFRYLLARGQTGLSVAFDLPTQLGYDSDDPLAEG
                     EVGRTGVAIDSLADMQALLDG"
     gene            complement(413..1639)
                     /locus_tag="VFO88_08095"
     CDS             complement(413..1639)
                     /locus_tag="VFO88_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9545524.1"
                     /translation="MVDAGYLPGERYEQIVGRPLGLEHGTLYETERERYFFSYVRRLL
                     VERYGEERVRSGGLRVHTTVSLPLQREARQAIRSTLDRGDDPAAAIVAVNPRNGAIRA
                     MTAVAPGERGLQFNLAEQGRRQSGSAFKTFVLAEAVRRGIHPDRTQYLSAPFTYTPPG
                     AEEWEVSTYGGDYHGPSTIAAATLRSDNTVYARLTVDLGPESVAQLAHDMGIRTELQP
                     VASIGLGSNSVSPLELASAYATLAAGGVYREPFAIRRVVLPNGRVHGADEWGRGERRR
                     VLTDGEAAVVTRILAQNMTSGTGTGAQIGRPAAGKTGTTDRHTDAWFAGYTPRLASAV
                     WVGYPRAAQEMTSVHGITVAGGTFPATIWGRFMGPALEGTPADAWPVPSTSPEWKQWR
                     GEHQFDGRRAAADDDD"
     gene            complement(1643..2425)
                     /locus_tag="VFO88_08100"
     CDS             complement(1643..2425)
                     /locus_tag="VFO88_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HET9545525.1"
                     /translation="MGARGNGNGHVPRPAGRRRRRLHRQRAVRTKRVAALLVALAALA
                     STLAVVAVSGGAYTAVTTWRDSCSIDDLRPAELGENSFVFAANGRLLGTIRAERNRQP
                     VPLERMSPWVERATLAIEDRRFYEHDGLDYEGIVRAAVRNLEAGEIVEGGSTITQQLV
                     RSLYTGDERSWERKGKEACLALQLEEEWTKEEILETYLNRVFFGNRAYGMQAAARTYF
                     ATSARELGPAQAALIAGLAQAPSAYDPFRRPEAALARRNTVL"
     gene            2484..3122
                     /locus_tag="VFO88_08105"
     CDS             2484..3122
                     /locus_tag="VFO88_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET9545526.1"
                     /translation="MHIVVKLFAGLREQAGTGERELDLPAGARVADAWDALGLGDAPA
                     GLLYAVNKAYAPADRELAEGDEVAFVPPVSGGAFRLVEGPLDAEAVAAEVAAEGAGAV
                     ATFTGTVRRHSRGRDVLHLEYEAYAGMAETVMAEIAAELEARHELCAIAIAHRVGRVE
                     IGEASVVIAVSAPHRAAALAACAEAIDTLKERVPLWKKEVYEGGEEWLGRGS"
     gene            3211..3822
                     /locus_tag="VFO88_08110"
     CDS             3211..3822
                     /locus_tag="VFO88_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9545527.1"
                     /translation="MRKLLVNTFLTLDGVMQAPGGPEEDRSGGFDHGGWSVNYWDERM
                     GEVMGEALSRPFALVLGRRTYDIFAAHWPHASEEEGAATFNEATKYVASRSNPTLEWA
                     NSELIEGDAAEGVAALKAEDGPELQVHGSSDLLQTLVRHGLVDEFRLWVFPVVVGSGK
                     RLFGDGTSAAGLRLVDSTVSSTGVVMGTYEPAGELVTGSFALE"
     gene            3876..4748
                     /locus_tag="VFO88_08115"
     CDS             3876..4748
                     /locus_tag="VFO88_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9545528.1"
                     /translation="MSGLPPDRYGLEPQPPASAPEPDEHVLRDYRPIQPESRFRELAR
                     RLWMPFAAIGLLVWKFKAVALAAFKLKIFTTSASMLVSIGAYALFWGWKFAVGFVLLL
                     FVHELGHVLELRRQGVPATAPLFIPFLGAVVGMKQLPADAWREAQVALAGPILGTAGA
                     LAVWGAAEALDSELLLAIAFVGFFLNLFNLLPIVPLDGGRAVAAIHPSFWAVGLVVLV
                     GLLFVAPNPILLLVLVLGGLELWRRWKERREPGGADYYRVTPWQRLAVGVTYVGLAAG
                     LALAMSATFVERSL"
     gene            4878..5684
                     /locus_tag="VFO88_08120"
     CDS             4878..5684
                     /locus_tag="VFO88_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545529.1"
                     /translation="MEKSSQRKGFRGPSPAMVVACIALAVALSGVSYAASHLARNSVG
                     TVHLKDGAVTAAKIRANAVNGSKVANNSLTGADINEGTLQGVDAATVGGIEPDDLLVK
                     TPDHTDAFVAAGGSWVDADGADRTPVNATLANSTQVCTMTGASVSGASAAYQDVHLPQ
                     GALITGIVAEYIDDAGTTNSNGSVGLVRQPLFSEQQGSQLEVFTAGLINTGSGLAAGL
                     PGIGTDSSPAGNNFANATVDNTRYAYSLQANPGTNTGVAFCNVKVTYELP"
     gene            5811..6506
                     /locus_tag="VFO88_08125"
     CDS             5811..6506
                     /locus_tag="VFO88_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET9545530.1"
                     /translation="MEDRKMLESRDGDRRRVKRIAAEFFEGFEAVAQIPSPAVSMFGS
                     ARVREGHFAYDYARKTARCFAEKGWSVVTGGGPGVMEAANRGAKEGGGISVGFNIDLP
                     HEQYANPYLDLEVTFAHFYARKTMFVKAAEAFVIFPGGFGTLDELFESLTLIQTGKIL
                     DFPVVLVGSRYWGELLTWIRERPLKEGIVSPDDVELLYLTDDPDEAVRTVIERHAQRA
                     GTSPASPAKADAE"
     gene            6552..7862
                     /gene="selA"
                     /locus_tag="VFO88_08130"
     CDS             6552..7862
                     /gene="selA"
                     /locus_tag="VFO88_08130"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET9545531.1"
                     /translation="MEPRALRDLPSVDALLAEEPVQALAREHGREVALAAARAALARA
                     REEIRAGHPPGELAARVEAEAAALLRPRLRRVLNATGVVVHTNLGRAPLPEAALERIG
                     EVARGYSNLELDLGAGVRGSRHDHLAPALRRLTGAEDALVVNNNAGALLLALAALAEG
                     REVVVSRGELIEIGDGFRIPDVLARSGARLVEVGTTNRTRVRDYERALGDATAVLLRV
                     HQSNFRMVGFAERPSLRELAALAERRGVVLVDDLGSGAVTPLGDEPSPRESVEAGAHL
                     VAFSGDKLLGGPQAGIVAGRAELVERLRRHPLQRALRADKLQLAALEGTLAAHRTGAV
                     PVLRMAGEAAEDVRARAERLAGATDGEVVETVARIGGGALPLAELPSFACALPVALAV
                     PLRLGEPPVVGIVRDERLLLDCRTLTDAEADEAAGAVERARSER"
BASE COUNT         1004 a         2930 c         2865 g         1102 t
ORIGIN      
        1 ccgtcgagca gggcctgcat gtcggcgagc gagtcgattg cgaccccggt ccggccgacc
       61 tcgccctcgg cgagcgggtc gtcggagtcg tagccgagct gcgtcggcag gtcgaacgcg
      121 acggacaggc cggtctgccc ccgcgcgagc agataccgga agcgcgcgtt cgtctcctcc
      181 gccgacgcga agccggcgta ctggcggatc gtccacggcc ggccgcggta catgtcccgg
      241 tagggcccgc gcgtgaaggg gaactcgccc ggggcctcga gctcgagacc ggcgacgtcc
      301 tcgtccgtgt agacgggctt gacctcgatt cccgagtcgg tctcgcgccg gtccggcgtg
      361 gtcgccatcg ggcgaatcgt aggtggtggc gggcccaggc gggcgcggcg ggtcagtcgt
      421 cgtcgtccgc cgccgctcgg cgaccgtcga actggtgctc gccgcgccac tgcttccact
      481 cgggcgacgt cgacggcacc ggccacgcgt cggccggcgt gccctcgagc gccggcccca
      541 tgaagcgccc ccagatcgtc gcggggaacg tcccgcccgc gaccgtgatc ccgtggacgc
      601 tcgtcatctc ctgcgcggcc cgcggatatc cgacccacac cgcgctcgcg agccgcggcg
      661 tgtacccggc gaaccaggcg tccgtgtgcc ggtcggtcgt ccccgtcttc cccgcggccg
      721 ggcggccgat ctgcgcgccg gttcccgttc ccgacgtcat gttctgcgcg aggatccgcg
      781 tcacgaccgc cgcctctccg tccgtgagca cgcgccgccg ctcgccgcgg ccccactcgt
      841 ccgccccgtg cacccgcccg ttcgggagga cgacgcggcg gatcgcgaac ggctcgcggt
      901 agaccccgcc cgcggcgagc gtcgcgtacg ccgacgcgag ctcgagcggc gagacggagt
      961 tcgacccgag cccgatggac gcgaccggct ggagctccgt ccgaatcccc atgtcgtgcg
     1021 cgagctgcgc gacgctctcc ggcccgaggt cgaccgtcag ccgcgcatag accgtgttgt
     1081 cggagcggag cgtcgccgcg gcgatcgtcg acggcccgtg gtagtcgccc ccgtacgtcg
     1141 acacctccca ctcctccgcg ccgggcggcg tgtacgtgaa cggcgccgag aggtattgcg
     1201 tccggtccgg gtggatcccg cgtcgaaccg cctcggcgag gacgaacgtc ttgaacgccg
     1261 agccggactg ccgccggccc tgctcggcga ggttgaactg gagcccgcgc tccccgggcg
     1321 cgaccgcggt catcgcgcgg atggcgccgt tccgcgggtt caccgcgacg atcgccgccg
     1381 ccggatcgtc gccgcggtcg agcgtggagc ggatcgcctg ccgggcttcc cgctggagcg
     1441 gaaggctcac ggtcgtgtgc acgcggaggc cgccgcttcg cacgcgctcc tcgccgtagc
     1501 gctcgacgag cagccgccgg acgtacgaga agaagtagcg ctcgcgctcc gtctcgtaga
     1561 gcgtcccgtg ctcgagcccg agcggccgcc cgacgatctg ctcgtagcgc tccccgggga
     1621 gatagcccgc gtcgaccatc gcctagagca ccgtgttgcg ccgggcgagc gccgcctcgg
     1681 gccgcctgaa cgggtcgtac gccgacggcg cctgcgcgag cccggcgatg agcgccgcct
     1741 gcgcggggcc gagctcgcgc gcgctcgtgg cgaagtacgt ccgcgccgcc gcctgcatgc
     1801 cgtaggcccg gttcccgaag aagacccggt tcaggtacgt ctcgaggatc tcctccttcg
     1861 tccactcctc ctcgagctgg agggcgaggc aggcctcctt ccccttgcgc tcccacgagc
     1921 gctcgtcgcc ggtgtagagg ctgcggacga gctgctgcgt gatcgtcgag ccgccctcga
     1981 cgatctcgcc cgcctcgagg ttgcgcaccg ccgcgcggac gatcccctcg tagtcgaggc
     2041 cgtcgtgctc gtagaaccgg cgatcctcga tcgcgagcgt cgcgcgctcg acccaggggc
     2101 tcatccgctc gagcgggacg ggctggcggt tccgctccgc tcggatcgtg ccgaggagcc
     2161 gcccgttcgc ggcgaagacg aacgagttct cgccgagctc ggccggccgg aggtcgtcga
     2221 tggagcagct gtcccgccac gtcgtgacgg ccgtgtacgc gccgcccgac accgcgacga
     2281 ccgcgagcgt ggacgcgagc gccgcgagcg cgacgaggag cgccgcgacc cgcttcgtcc
     2341 gcacggcccg ctgccggtgc agccgccggc ggcggcgccc tgcgggccgc gggacgtgtc
     2401 cgtttccgtt tccgcgtgct cccatccgtt ctcgacttcc gggcacgggc cgggcggaaa
     2461 cccgcgcacc gtacgctacg cccgtgcaca ttgtcgtcaa gctcttcgcg ggcctgcggg
     2521 agcaggcggg aaccggcgag cgggagctcg accttccggc cggcgcgcgc gtggccgacg
     2581 cctgggacgc gctcgggctc ggcgacgctc cggcggggct cctctacgcc gtgaacaagg
     2641 cgtacgcgcc ggccgaccgg gagctcgccg agggcgacga ggtcgcgttc gtcccgccgg
     2701 tctcgggcgg cgccttccgg ctcgtcgagg gcccgctcga cgccgaggcg gtcgccgccg
     2761 aggtggccgc cgagggggcc ggcgccgtcg ccacgttcac ggggacggtg cgccggcact
     2821 cccgcgggcg cgacgtcctc cacctcgagt acgaggcgta cgccgggatg gccgagacgg
     2881 tcatggccga gatcgccgcc gagctcgagg cgaggcacga gctctgcgcg atcgcgatcg
     2941 cgcaccgggt cgggcgcgtc gagatcggcg aggcgagcgt cgtcatcgcc gtgtccgcgc
     3001 cgcatcgcgc cgccgccctc gccgcctgcg cggaggcgat cgacacgctg aaggagcgcg
     3061 tcccgctctg gaagaaggag gtctacgaag gcggcgagga gtggctcggc cgaggctcgt
     3121 aggggccgct gcgatgagtc tcgcgcgacc gcgctgtcct acatgcgacg gcgattcgcc
     3181 ggccgatctc caagcgaaag gactcaagcg atgagaaagc ttctcgtcaa cacgttcctc
     3241 accctcgacg gcgtcatgca ggcgcccggc ggccccgagg aggaccgaag cggcggcttc
     3301 gaccacggcg gctggtcggt gaactactgg gacgagcgga tgggcgaggt catgggcgag
     3361 gcgctcagcc ggccgttcgc gctcgtcctc gggcgcagga cgtacgacat cttcgccgcg
     3421 cactggcccc acgcctccga ggaggagggt gcggcgacgt tcaacgaggc gacgaagtac
     3481 gtcgcgtcgc ggagcaaccc gacgctcgaa tgggcgaact ccgagctgat cgagggcgac
     3541 gcggccgagg gcgtcgccgc gctcaaggca gaagacggcc cggagctcca ggttcacggc
     3601 agctccgacc tgctccagac cctcgtacgg cacggcctcg tggacgagtt ccggctgtgg
     3661 gtgttcccgg tcgtggtcgg gtcaggcaaa cgcctgttcg gggacggaac ctcggccgcg
     3721 gggctgcggc tcgtcgacag caccgtctcg tcgaccggcg tggtgatggg gacgtacgag
     3781 cccgcgggcg agctcgtcac cggctccttc gcgctcgagt agccgacgcc cgcacggggg
     3841 gcggcggccc ggcggtcggc atctacgatg cgtaggtgag cggcctacct cccgaccgct
     3901 acggcctcga gccgcagccg cccgcctcgg cgcccgagcc ggacgagcac gttctccgcg
     3961 actaccgacc gatccagccg gagagccgct tccgcgagct cgcgcggcga ctctggatgc
     4021 cgttcgcggc gatcggcctc ctcgtctgga agttcaaggc ggtcgcgctc gccgcgttca
     4081 agctgaagat cttcacgacg tccgcgtcga tgctcgtgtc gatcggcgcc tacgcgctct
     4141 tctggggctg gaagttcgcg gtcggcttcg tcctgctcct cttcgtgcac gagctcggtc
     4201 acgtgctcga gctgcgccgg cagggcgtgc ccgccacggc gccgctcttc atcccgttcc
     4261 tcggcgcggt cgtggggatg aagcagctcc ccgccgacgc gtggcgtgag gcgcaggtcg
     4321 cgctcgccgg gccgatcctc gggacggccg gggcactcgc cgtctgggga gccgccgagg
     4381 cgctcgactc cgagctcctg ctcgcgatcg cgttcgtcgg gttcttcctg aacctcttca
     4441 acctgctccc gatcgtcccg ctcgacggcg ggcgggcggt ggcggcgatc catccgtcgt
     4501 tctgggccgt cgggctcgtc gtgctcgtcg gcctcctgtt cgtcgccccg aacccgatcc
     4561 tcctgctcgt cctcgtcctc ggcggcctcg agctctggcg gcgctggaag gagcgccgcg
     4621 agccgggcgg cgccgactac tacagagtca ccccgtggca gcgcctggcg gtcggcgtca
     4681 cgtacgtcgg tctcgccgcc ggcctcgccc tcgcgatgag cgccacgttc gtcgagcgct
     4741 cgctctgagc gtcccctgcg cttttccaac taagcccttt tctcggggcc cagagtgtgg
     4801 gagactactg cggctcggcc ccggcgatcc cagcgcggag cgcaagcatc ggtcattcag
     4861 gacgggaggt tttgttcatg gagaaaagct cgcagcggaa gggctttcgc ggtccttcgc
     4921 ccgcgatggt cgtcgcgtgc attgcgctcg cagtcgccct gagcggtgtc agctatgccg
     4981 cgtcgcacct tgcgcggaac agcgtgggca cggtgcacct caaggacggc gcggtgactg
     5041 cggcgaagat cagggcgaac gcggtcaacg ggtcgaaggt cgcgaacaac tccctgacgg
     5101 gcgcggacat caacgagggc accctccagg gtgtcgacgc cgcgaccgtg ggcgggatcg
     5161 agcccgacga cctcctcgtg aagacccccg accacacgga cgccttcgtg gccgccggcg
     5221 gtagctgggt cgacgcggac ggcgccgatc gcacgcccgt gaacgcgacg ctcgcgaact
     5281 cgacgcaggt ctgcaccatg accggcgcgt cggtgtccgg tgcgtccgcc gcctaccagg
     5341 acgtccacct gccccagggc gcgctcatca ccgggatcgt ggcggagtac atcgacgacg
     5401 cgggcacgac gaactcgaac gggagcgtcg gcctcgtgcg gcagcccctg ttctccgagc
     5461 agcagggctc gcagctcgag gtcttcacgg ccgggctcat caacaccggc tcgggtctcg
     5521 cggccgggct gccgggcatc ggcacggatt cctcgccggc cggcaacaac ttcgcgaacg
     5581 cgaccgtgga caacacgcgc tacgcctact cgctgcaggc gaaccccggc acgaacacgg
     5641 gcgtcgcgtt ctgcaacgtg aaggtcacgt acgagctgcc gtaaccgaac ctgaaggagc
     5701 gggtcaccga cccgtccacc gaatgctcga gcgaggggcg ccggaaggcg cccctcgtct
     5761 cttctccccc gcgcgcgtgc gagcgggcgg ccggggctac catcctctcc gtggaagacc
     5821 ggaagatgct cgagagccgc gacggcgacc gccgccgtgt caagcgcatc gccgcggagt
     5881 tcttcgaggg cttcgaggcg gtcgcacaga tcccaagccc ggcggtgtcg atgttcggct
     5941 ccgcgcgcgt gcgcgagggc cacttcgcct acgactacgc gcgcaagacg gcccgctgct
     6001 tcgcggagaa gggctggtcg gtcgtgaccg gcggcgggcc cggcgtgatg gaggcggcga
     6061 accggggcgc gaaggagggc ggcggcatct ccgtcggctt caacatcgac ctcccgcacg
     6121 agcagtacgc gaacccctat ctcgacctcg aggtgacgtt cgcccacttc tacgcgcgaa
     6181 agacgatgtt cgtgaaggcg gccgaggcgt tcgtgatctt cccgggcggg ttcgggacgc
     6241 tcgacgagct cttcgagtcg ctgacgctca tccagacggg caagatcctc gacttccccg
     6301 tcgtgctcgt cggctcgcgc tactggggcg agctcctgac ctggatccgc gagcggccgc
     6361 tcaaggaggg gatcgtgagc ccagacgacg tcgagctcct ctacctcacg gacgatcccg
     6421 acgaggccgt ccgcaccgtg atcgagcggc acgcccagcg cgcggggacg tcgccggcct
     6481 cgcccgcgaa ggccgacgcc gagtagccag gcgccgcggc ccagtgcgag gcgcggccta
     6541 tcatccgtcg catggagccg cgcgccctcc gcgacctgcc ctccgtcgac gccctgctcg
     6601 ccgaggagcc ggtgcaggcg ctcgcgcggg agcacgggcg cgaggtcgcg ctcgccgcgg
     6661 cgcgcgcggc gctcgcgcgg gcgcgggagg agatccgcgc cggccatccg ccgggcgagc
     6721 tcgcggcgcg ggtcgaggcc gaggcggccg cgctcctccg gccgcgcctg cgccgcgtcc
     6781 tgaacgcgac cggggtcgtc gtccacacga acctgggccg cgcgccgctt ccggaggcag
     6841 cgctcgagcg gatcggcgag gtcgcgcgcg gctactcgaa cctcgagctc gacctcggcg
     6901 ccggcgtccg cggctcgcgc cacgaccacc tcgcccccgc gctgcgccgg ctgacgggcg
     6961 ccgaggacgc gctcgtcgtg aacaacaacg cgggcgcgct gctcctcgcg ctcgccgccc
     7021 tcgccgaggg ccgcgaggtg gtcgtctcgc gcggcgagct gatcgagatc ggggacggct
     7081 ttcgcatccc cgacgtgctc gcccgctccg gggcccggct cgtcgaggtc gggacgacga
     7141 accggacgcg cgtccgcgac tacgagcggg cgctcggcga cgcgacggcc gtgctgctgc
     7201 gcgtgcacca gtcgaacttc cggatggtcg ggttcgccga gcgcccgtcg ctccgcgagc
     7261 tcgcggcgct cgccgagcgg cgcggggtcg tgctcgtcga cgacctcggc tccggcgcgg
     7321 tcacgccgct cggggacgag ccgtcgccgc gggagagcgt cgaggccggc gcgcacctcg
     7381 tggccttctc cggggacaag ctcctcggcg gcccgcaggc gggcatcgtc gccgggcgcg
     7441 ccgagctcgt cgagcggctg cgccgccatc cgctccagcg cgcgctccgg gcggacaagc
     7501 tccagctggc cgcgctcgag gggacgctcg ccgcgcaccg gaccggcgcc gtgccggtgc
     7561 ttcgcatggc gggcgaggcg gccgaggacg tccgggcccg ggccgagcgg ctcgccggcg
     7621 cgacggacgg cgaggtcgtc gagacggtcg cgcggatcgg cggcggggcg cttccgctcg
     7681 ccgagctccc gagcttcgcg tgcgcgctcc cggtcgcgct cgccgtcccg ctccgcctcg
     7741 gcgagccgcc cgtcgtcggc atcgtccgcg acgagcggct cctcctcgac tgccgcacgc
     7801 tcacggacgc cgaggcggac gaggcggccg gcgccgtcga gcgcgcccgc tcggagcggt
     7861 gacccggccg ctcaccgtcg gcacggcggg gcacatcgac c
//
LOCUS       DASTFC010000274         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1011827,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000274 DASTFC010000000
VERSION     DASTFC010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011827"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..778
                     /gene="ccsA"
                     /locus_tag="VFO88_08135"
     CDS             101..778
                     /gene="ccsA"
                     /locus_tag="VFO88_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9545532.1"
                     /translation="MRNNLGPALAAGALLVLGTGLVLALFWAPEDADQGFSQRIFYVH
                     VPIALTAYACFGWGAWKALRLLWTREARYDLESYTAIHMGTIFGVLVLVTGSIWAKVS
                     WGIWWSWSERQLVLFLVLFLFYSAYFMLRYSTDPGPRRANMSAVYALFGVVLIPVSFL
                     AIRLAENFIHPVVFTRDGPQMAGSMFFVFCVCLAGMLLLAAALYQAELAGKRLDARVR
                     ELREALA"
     gene            775..969
                     /locus_tag="VFO88_08140"
     CDS             775..969
                     /locus_tag="VFO88_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545533.1"
                     /translation="MSRGETYVFAAYLALFVVVLGWLVIHAYKIARLSRELAELVELA
                     RRRDGKAPAPDAAEARVAGQ"
     gene            938..1774
                     /gene="hemC"
                     /locus_tag="VFO88_08145"
     CDS             938..1774
                     /gene="hemC"
                     /locus_tag="VFO88_08145"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9545534.1"
                     /translation="MLLRLGSRGSRLALTQAEAAADLLRAALGDVDVALVPITTAGDR
                     DRSRPFGEIGARGVFVKEIEEALLAGRIDVAVHSAKDMTSTDTDGLAVAAYLAREDPR
                     DALCGADGVRRGMRVGTASARRKAQLLALEPSLSVEPLRGNVDTRLRKRDERGLDAIV
                     LAAAGLVRLGRENEIGHRFDPEELVPEAGQGAVALQTRTGDEGLVAALDDPETRRRVA
                     SERACVAAIGAGCLAPVGAHHDGSELVALIAAEDGAWVERRRGDDPLGLAEELLAATA
                     GR"
     gene            1771..>2050
                     /locus_tag="VFO88_08150"
     CDS             1771..>2050
                     /locus_tag="VFO88_08150"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET9545535.1"
                     /translation="MRVALTRAAGGNAALAERLRAAGLEPVECPLVAVRPLPGPPVDV
                     RPYDRLCLTSRHGVEALFARGFEGELPPVAVVGPGTAEALRAHGVEPEI"
BASE COUNT          224 a          750 c          766 g          310 t
ORIGIN      
        1 ggcgcgagcg tcgagccgga ccccggccgc tacctcgcct tcctcgccct gtacgacctc
       61 atctttgcga tccttgcgtg ggcgagcttt gactacgtcg ttgcggaata acctcgggcc
      121 ggcgctcgcc gccggcgccc tgctcgtcct cgggacgggg ctcgtcctcg cgctcttctg
      181 ggcgccggag gatgccgacc agggcttcag ccagcgcatc ttctacgtcc acgtcccgat
      241 cgcgctgacc gcctacgcgt gcttcggctg gggcgcgtgg aaggcgctgc gccttctctg
      301 gacgcgtgag gcgcgctacg acctcgagag ctacacggcc atccacatgg ggacgatctt
      361 cggcgtcctc gtcctcgtga cgggctcgat ctgggcgaag gtctcgtggg ggatctggtg
      421 gagctggagc gagcggcagc tcgtgctctt cctcgtcctc ttcctcttct actcggccta
      481 cttcatgctc cgctactcga ccgacccggg gccgcggcgg gcgaacatgt ccgcggtgta
      541 cgcgctgttc ggcgtcgtgc tcatcccggt ctcgttcctc gcgatccggc tcgcggagaa
      601 cttcatccac ccggtcgtct tcacgcgcga cgggccgcag atggcgggaa gcatgttctt
      661 cgtcttctgc gtctgtctcg ccgggatgct cctgctcgcc gccgcgctct accaggcgga
      721 gctcgccggg aagcggctcg acgcgcgcgt gcgcgagctg cgggaggcgc tcgcgtgagc
      781 cgcggcgaga cgtacgtctt cgcggcgtac ctcgcgctct tcgtcgtcgt gctcgggtgg
      841 ctcgtcatcc acgcgtacaa gatcgcgcgc ctctcgcggg agctcgcgga gctcgtcgag
      901 cttgcgcggc gccgcgacgg gaaggcgccg gctcccgatg ctgctgaggc tcgggtcgcg
      961 gggcagtagg ctcgcgctca cgcaggcgga ggcggccgcc gacctgctgc gcgcggcgct
     1021 cggcgacgtc gacgtcgccc tcgtcccgat cacgaccgcg ggcgaccgcg accgctcgcg
     1081 cccgttcggc gagatcggcg cgcgcggcgt cttcgtcaag gagatcgagg aggcgcttct
     1141 cgccgggcgc atcgacgtcg ccgtgcactc ggcgaaggac atgacctcga cggacacgga
     1201 cgggctcgcg gtcgcggcct acctcgcccg cgaggacccg cgcgacgcgc tctgcggcgc
     1261 ggacggtgtg cgcaggggga tgcgcgtcgg gacggcctcg gcgcggcgga aggctcagct
     1321 cctcgcgctc gagccctcgc tctcggtcga gccgctgcgc gggaacgtcg acacgcgcct
     1381 gcgcaagcgc gacgagcgcg gcctcgacgc gatcgtgctc gcggcggcgg ggctcgtccg
     1441 gctcggccgc gaaaacgaga tcgggcaccg cttcgacccc gaggagctcg tcccggaggc
     1501 gggccaggga gcggtcgcgc tgcagacgcg aacgggagac gaggggctcg tcgcggcgct
     1561 cgacgacccg gagacccggc ggcgggtcgc ctccgagcgc gcgtgcgtcg ccgcgatcgg
     1621 ggccggctgc ctcgcgccgg tcggcgcgca tcacgacggc tcggagctcg tcgccctgat
     1681 cgcggccgag gacggggcgt gggtcgagcg ccggcgcggc gacgacccgc tcgggctcgc
     1741 cgaggagctc ctcgccgcga ccgccggccg atgagagtcg cgctcacgcg ggcggcgggc
     1801 gggaacgccg cgctggccga gcggctccgc gcggccgggc tcgagccggt cgagtgcccg
     1861 ctcgtcgccg tccgtccgct gccgggccca ccggtcgacg tgcgccccta cgaccggctc
     1921 tgcctcacga gccgccacgg ggtcgaggcg ctcttcgcgc gcggcttcga gggcgagctg
     1981 ccgcctgtcg cggtcgtcgg gccgggcacg gcggaggcgc tgcgcgcgca cggcgtcgag
     2041 ccggagatcg
//
LOCUS       DASTFC010000275         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_533983,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000275 DASTFC010000000
VERSION     DASTFC010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533983"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFO88_08155"
     CDS             complement(<1..564)
                     /locus_tag="VFO88_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9545536.1"
                     /translation="MTRPSAQAVLDGLRRDEEAIASLLALLAEAESPTHDEEAQRAVF
                     ALLAGPLEELDFAVRRLPGGHLYARPRTRRRGAATQLLVGHLDTVWPVGTVAGMPVRR
                     DGDRLYGPGVFDMKGGLAITVYALRALAAHDVEPAATPLVFVAADEEAGSDDSRRTLL
                     RLARSAARAFVLEPPYGPSGRLKTARKG"
     gene            complement(574..1182)
                     /locus_tag="VFO88_08160"
     CDS             complement(574..1182)
                     /locus_tag="VFO88_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HET9545537.1"
                     /translation="MSGRPSADELVRLDVGAPLWERVFHVAPLVLVGTREGGGYDLAP
                     KHMAMPLGRDGWFCFACTPRHATYWNAKHHGAFTVSFPTPELVVETGLAASGREGEGR
                     KRGLAALDTFPATTVDGVLVAGCLLHLECELDRIVDGFGDASLVVGKIVAALADERVL
                     ALDDADPADVVRRAPLLAYVSPGRFARVGDTLSFPFPADFSR"
     gene            complement(1366..1842)
                     /locus_tag="VFO88_08165"
     CDS             complement(1366..1842)
                     /locus_tag="VFO88_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9545538.1"
                     /translation="MKHIVVATDGSHGAGEAVRMGLEIAAAEGARVTFVHAFIPDELL
                     YAGRVPGVVMPAPAPAEKAPEAENDGPLLAASLEAKKAGVEHDTELVGGDPVGAILAC
                     ADEHDADLIVVGSRGHGALVSALLGSVSKAVVTRAKRPVLVVRAAPEAEQTAGKAA"
     gene            1978..>2957
                     /locus_tag="VFO88_08170"
     CDS             1978..>2957
                     /locus_tag="VFO88_08170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9545539.1"
                     /translation="MQTEAAVRDVILRDGTTLRLRPPRREDSDAVGEFFAALSEESLY
                     LRFHGGFRPDDAWIRGLLEPDWDERGVLLAVRDDRVVAVAGYTRLRDPEVAEVAFAVA
                     DELQRRGVGTRMLEQLAGLAAASGVRRFLAEVAVENVGMLRVFGDAGFAETFRRSGDE
                     VTVEVELDPTVEYTEAVDLRDHVAVAASLRPFFAPRSVAVVGASPRRGTIGGELFRNV
                     AASGFPGPAYPVNRGGQEVEGREGYESLDDVPGDVDLVFVCVPGEAALSVAETALRRG
                     TRALCVISAGFAEHGEEGRRRQDELLALVRAHGARMVGPNCLGIALADPPL"
BASE COUNT          346 a         1094 c         1140 g          377 t
ORIGIN      
        1 gcccttccgc gccgtcttca gccggccgga cgggccgtac ggcggctcga ggacgaacgc
       61 gcgcgccgcg gaacgggcga gccggagcag ggtccggcgg gagtcgtcgc tccccgcctc
      121 ctcgtcggcc gcgacgaaga cgagcggcgt ggcggccggc tccacgtcgt gtgcggcaag
      181 cgcccgcagc gcgtagaccg tgatcgccag gccgcccttc atgtcgaaga cgcccggccc
      241 gtagagtcgg tcgccgtcgc gccggacggg catccccgcg accgttccga ccggccacac
      301 ggtgtcgagg tgcccgacga gcagctgcgt cgccgcgccg cgccgccgcg tccgcgggcg
      361 cgcgtagaga tggccgcccg gcaggcggcg caccgcaaag tcgagctcct cgagcggccc
      421 cgcgagcagt gcgaagacgg cgcgctgcgc ctcctcgtca tgggtcggcg actcggcctc
      481 ggccagcagc gcgaggaggg aggcgatcgc ctcctcgtca cggcgaaggc cgtccaggac
      541 agcctgcgcg ctcggccgcg tcatcggtat cgcctacctc gagaagtcgg ccggaaacgg
      601 gaacgagagc gtgtcgccga cgcgcgcgaa gcgtcccggg ctcacgtagg cgaggagcgg
      661 cgcgcggcgg acgacgtccg ccgggtcggc gtcgtcgagc gcgagtaccc gctcgtcggc
      721 gagcgcggcg acgatctttc cgacgacgag gctcgcgtcg ccgaagccgt cgacgatccg
      781 gtcgagctcg cactcgaggt ggaggaggca gccggcgacg agcaccccgt ccaccgtggt
      841 cgcgggaaac gtgtcgagcg ccgcgaggcc gcgcttgcgc ccttcgccct cgcggcccga
      901 cgcggcgagg ccggtctcga cgacgagctc gggcgtcggg aagctcacgg tgaacgcgcc
      961 gtggtgcttc gcattccagt acgtcgcgtg gcgcggcgtg caggcgaagc agaaccagcc
     1021 gtcgcggccg agcggcatcg ccatgtgctt cggagccagg tcgtagccgc cgccctcgcg
     1081 cgtgccgacg agcacgagcg gggcgacgtg gaacacgcgc tcccagagcg gggccccgac
     1141 gtcgaggcga acgagctcgt ccgcggacgg gcggccgctc accgcgcccg cctccccggg
     1201 gcgtgaccgg aattcgcccg gcgctcggag ggccgatgcg ccgggcgatc cggtacctcg
     1261 cgcgcggccc gtcgccgcaa cgggcgcgcg cgagactcgg gaaaccggcc tacgccgggt
     1321 gcgctccggg actcgccggt cgggtcgtgg gcgcgcacgg cggcctcacg cggccttgcc
     1381 ggccgtctgc tccgcttccg gcgcggccct gacgacgagg acggggcgct tcgcacgggt
     1441 cacgaccgcc ttcgagacgc tcccgaggag cgccgagacg agcgcgccgt gtccgcgcga
     1501 cccgacgacg atcagatcgg cgtcgtgctc gtccgcgcac gcgaggatcg cgccgaccgg
     1561 gtctccgccc acgagctccg tgtcgtgctc gacgcccgcc ttcttcgcct cgagcgaggc
     1621 ggcgaggagc gggccgtcgt tctcggcctc gggcgccttc tccgccgggg cgggcgccgg
     1681 catgacgacg ccgggcacgc ggcctgcgta gagcagctcg tccgggatga acgcgtgcac
     1741 gaacgtgacg cgggctccct cggcggcggc gatctcgagc cccatgcgga cggcctcgcc
     1801 cgcgccgtgc gagccgtcgg tggcgacgac gatgtgcttc atggcgcctc ccttcgtttc
     1861 gccctcagtg tccgtcgcgc ggtgccgggc gggcatcggg gaagccaccc tcccgcgtgc
     1921 gggatttcca cagtcccgaa ctgcgtgcgg cgggcccggt gcggtaatgc tgtccgggtg
     1981 cagaccgagg ccgccgtccg cgacgtcatc ctccgcgacg ggacgacgct gcggctgcgc
     2041 ccgccgcggc gcgaggactc ggacgccgtg ggggagttct tcgcggccct ctcggaggag
     2101 agcctctacc tgcgcttcca cggcggcttc cggccggacg acgcctggat ccgcggcctg
     2161 ctcgagccgg actgggacga gcggggcgtg ctcctcgccg tgcgggacga ccgggtcgtc
     2221 gccgtcgcgg gctacacgcg ccttcgcgac ccagaggtgg ccgaggtcgc gttcgcggtc
     2281 gcggacgagc tccagcggcg cggggtcggg acgcggatgc tcgagcagct cgcgggcctc
     2341 gcggcggcct ccggcgtgcg gcgcttcctc gcggaggtgg cggtcgagaa cgtcgggatg
     2401 ctccgcgtct tcggcgacgc cgggttcgcg gagacgttcc ggcgctcggg cgacgaggtg
     2461 accgtcgagg tcgagctcga cccgaccgtc gagtacaccg aggccgtcga cctgcgcgac
     2521 cacgtcgccg tcgccgcgtc cctccggccg ttcttcgcgc cgcggagcgt cgctgtcgtc
     2581 ggcgcctcgc cgcggcgcgg gacgatcggc ggggagctct tccgcaacgt cgccgcctcg
     2641 ggcttcccgg ggcccgcgta tcccgtcaac cggggcgggc aggaggtcga ggggcgcgag
     2701 ggctacgagt cgctcgacga cgtgcccggc gacgtcgacc tcgtgttcgt ctgcgtgccc
     2761 ggcgaggcgg cgctctccgt ggccgagacg gctctccggc gcgggacgcg cgcgctctgc
     2821 gtcatctcgg cggggttcgc ggagcacggc gaggaggggc ggcgccggca ggacgagctc
     2881 ctcgcgctcg tccgcgcgca cggggcgcgg atggtcggcc cgaactgcct cgggatcgcg
     2941 ctcgccgatc ccccgct
//
LOCUS       DASTFC010000276         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_385857,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000276 DASTFC010000000
VERSION     DASTFC010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385857"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFO88_08175"
     CDS             <1..1227
                     /locus_tag="VFO88_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9545540.1"
                     /translation="FRGIPLDEVSTSMTINAPAAMLLAFYICVGEEQGVPADKLRGTI
                     QTDILKEYIAQKEWIFPPEPSMRLCVDMIEHCAKEVPLWHPVSISGYHIREAGSTAAQ
                     ELAFTLADGFAYVEAALERGLDVDDFAPRLSFFFNAHIDFFEEIAKYRAARRIWAREL
                     RDRYGAKSPRSWLMRFHTQTAGVSLTAQQPEVNIVRTALEALAAVLGGTQSLHTNSFD
                     EALALPTEHAVKLALRTQQVIAHETGVASTIDPLGGSYFVEDLTNTLEAEAYDYFERI
                     RKLGGVIPAIKENFFQREIAEASFRFQSEVERGERVIVGVNRYQDADEKPIELLHIDA
                     ALEQQQIERVRALRGRRDSDAVERALARLKEDAAVDDRNLMPAILDASRAYVTMGEMC
                     DALRDVWGVWRETPVF"
     gene            1337..2554
                     /locus_tag="VFO88_08180"
     CDS             1337..2554
                     /locus_tag="VFO88_08180"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545541.1"
                     /translation="MEQTNGPRWERPDAPLGTLVYRAGLLSKDKLEAALEEGQRSGRR
                     LGEILLQKGWIDEKDLARLLAGQRGLQYVSLKGRGYELQAARLLTERVCRYHNAIPIE
                     LTGDTVLVAIADPTDESANDEIRDELKRAVNFVVATASEIRSAIDEVFGGAQAAQVAV
                     AGGAPTAISPIVAEHGLRIAAPVSELEPEPEPEPVPVAAEPVLTPVPAPDPEPVHEPD
                     PEPVAAAEPVAEPEPEYAPEPEPEPVLAADPEPLPTLPVLPGYEPAPEHAPEPAEHAP
                     EPAEPTIERNEDAYVSVAFHAAPDPIHEPEPVHEPEPVREPEPQPAAARGHDAYRLVL
                     NLEGGEEVDVALFADEAEAVAAARELVAGIAKDGEWPQVGTKFLPPDRIASVEVREKP
                     TDAGNGDQAGWAS"
     gene            complement(2551..>3184)
                     /locus_tag="VFO88_08185"
     CDS             complement(2551..>3184)
                     /locus_tag="VFO88_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9545542.1"
                     /translation="LLLDCGPGVLGRLRANGDVAVDAIAVTHFHLDHWGDLVPWAWLV
                     AYGQVPPRRTEVWLPPGGVRQLETFAGLWGNDSMFERAFELREYEPGTAFTAAGFEVE
                     ARRVRHYDLDAYGFRVREPGGPLLAYSGDTAPGEEIAAVAAGADLFLCEATLADANGE
                     PALRGHLTAEEALAAADGPVLLTHRPAELPAPDGTPLARDGLVVEVVRAG"
BASE COUNT          471 a         1216 c         1106 g          391 t
ORIGIN      
        1 ttccgcggca tcccgctcga cgaggtctcg acctcgatga cgatcaacgc gccggcggcg
       61 atgctgctcg cgttctacat ctgcgtcggc gaggagcagg gcgtcccggc ggacaagctg
      121 cgcgggacga tccagacgga catcctcaag gagtacatcg cgcagaagga gtggatcttc
      181 ccgcccgagc cctccatgcg cctctgcgtg gacatgatcg agcactgcgc gaaggaggtg
      241 ccgctctggc acccggtctc gatctcgggg taccacatcc gggaggccgg ctcgaccgcg
      301 gcgcaggagc tcgccttcac gctcgcggac gggttcgcct acgtcgaggc cgcgctggaa
      361 cgggggctcg acgtggacga cttcgcgccg cggctctcgt tcttcttcaa cgcccacatc
      421 gacttcttcg aggagatcgc gaagtaccgc gccgcgcgcc ggatctgggc gcgcgagctg
      481 cgggatcgct acggcgcgaa gagcccgcgc tcgtggctca tgcgcttcca cacccagacc
      541 gcgggcgtct cgctcacggc gcagcagccg gaggtgaaca tcgtccggac cgcgctcgag
      601 gcgctcgccg ccgtcctcgg cggcacgcag tcgctgcaca cgaactcgtt cgacgaggcg
      661 ctcgcgctcc ccaccgagca cgcggtgaag ctcgccctgc gaacgcagca ggtgatcgcg
      721 cacgagaccg gcgtcgcctc gacgatcgac ccgctcggcg gctcgtactt cgtcgaggac
      781 ctgacgaaca cgctcgaggc cgaggcgtac gactacttcg agcggatccg caagctcggc
      841 ggcgtcatcc cggcgatcaa ggagaacttc ttccagcgcg agatcgccga ggcgtccttc
      901 cgcttccaga gcgaggtcga gcggggcgag cgcgtcatcg tcggcgtgaa ccgctaccag
      961 gacgcggacg agaagccgat cgagctcctc catatcgacg cggcgctcga gcagcagcag
     1021 atcgagcgcg tccgggcgct gcgaggccgg cgcgactcgg acgcggtcga gcgggcgctc
     1081 gcgcgcctca aggaggacgc cgcggtcgac gaccggaacc tcatgcccgc gatcctcgac
     1141 gcctcgcgcg cctacgtcac gatgggcgag atgtgcgacg cgctccgcga cgtctggggc
     1201 gtctggcggg agacgcccgt cttctagcgg gctcccgtcg cgctatcacc cttcagggtg
     1261 attccgcgcc tccccggttg cgccgacttc cagagaaagc ccgccactgc ggccgagact
     1321 ggaggacgat cgccccatgg agcagacgaa cggaccccgc tgggagcgcc ccgatgcgcc
     1381 gctcggcacc ctggtctacc gcgccgggct cctctcgaag gacaagctcg aggccgccct
     1441 cgaggagggc cagcgctccg ggcgccggct cggcgagatc ctgctccaga agggttggat
     1501 cgacgagaag gacctcgcgc gcctgctggc cgggcagcgc gggctccagt acgtgagcct
     1561 caaggggcgc ggctacgagc tccaggcggc gcggctcctc accgagcgcg tgtgccgcta
     1621 ccacaacgcg attccgatcg agctgaccgg cgacaccgtc ctcgtggcga tcgccgaccc
     1681 caccgacgag agcgcgaacg acgagatccg cgacgagctc aagcgcgccg tgaacttcgt
     1741 cgtcgccacg gcgagcgaga tccgctcggc gatcgacgag gtcttcggcg gcgcgcaggc
     1801 tgcgcaggtc gccgtcgccg gcggcgcgcc gaccgccatc tccccgatcg tcgccgagca
     1861 cggcctccgc atcgccgctc ccgtttccga gctcgagccc gagccggagc ctgagccggt
     1921 gccggtggcc gccgagcccg tgctcacgcc ggtccccgcg cccgatcccg agcctgtcca
     1981 cgagccggac ccggagcccg tggccgcggc cgagccggtc gccgagccgg agcccgagta
     2041 cgccccggag cctgagccgg agcccgtcct cgcggccgat cccgagccgc tgcccacgct
     2101 tcccgtcctg cccggctacg agccggcccc ggagcacgct cccgagcccg cggagcacgc
     2161 gccggagccc gccgagccga cgatcgagcg caacgaggac gcatacgtga gcgtcgcgtt
     2221 ccacgccgcg cccgatccca ttcacgagcc cgagccggtt cacgagcccg agcccgtccg
     2281 cgagcccgag ccgcagcccg ccgccgcgcg cgggcacgac gcctaccggc tcgtgctcaa
     2341 cctcgagggc ggcgaggagg tcgacgtcgc gctcttcgcc gacgaggcgg aggcagtcgc
     2401 ggccgcccgc gagctcgtcg ccggcatcgc gaaggacggc gagtggccgc aggtcggcac
     2461 gaagttcctc ccgccggacc gcatcgcctc cgtcgaggtg cgcgagaagc cgacggacgc
     2521 cggaaacggc gaccaggccg gctgggcctc ctagccggcc cgcacgacct cgaccacgag
     2581 cccgtcccgc gcgaggggcg ttccgtccgg cgccggcagc tcggccgggc ggtgcgtcag
     2641 gagcaccggg ccgtccgccg ccgcgagcgc ctcctcggcg gtcaggtggc cgcgcagcgc
     2701 cggctcgccg ttcgcgtccg cgagcgtcgc ctcgcagagg aagaggtcgg ccccggccgc
     2761 gaccgccgcg atctcctcgc cgggcgcggt gtcgcccgag tacgcgagaa gcggtccgcc
     2821 cggctcgcgg actcggaagc cgtaggcgtc gaggtcgtag tggcgcaccc gccgggcctc
     2881 gacctcgaac ccggccgccg tgaacgccgt cccgggctcg tactcccgaa gctcgaacgc
     2941 ccgctcgaac atcgagtcgt tcccccagag ccccgcgaac gtctcgagct gccgcacgcc
     3001 gccgggcggg agccagacct ccgtccgccg gggcggcacc tggccgtacg cgacgagcca
     3061 ggcccaggga acgagatcgc cccagtgatc gaggtggaaa tgcgtcaccg cgatcgcgtc
     3121 gaccgcgacg tctccgttcg cgcgcagccg cccgaggacg ccgggcccgc agtcgagcag
     3181 gagc
//
LOCUS       DASTFC010000277         7252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_913218,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000277 DASTFC010000000
VERSION     DASTFC010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913218"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFO88_08190"
     CDS             <1..479
                     /locus_tag="VFO88_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9545543.1"
                     /translation="NEEALQQLAGLYLIRANEADERAERARSASGVAFFNVELYGNEL
                     SQAFGAGPFVEAQQQRTSEAYSAAALEAQQWHAEEAAIWEKLVDLQPEDSRYLLELGR
                     SKQQANEIPAAIEAYEKYIEVAPEDDPTAEQVEQLLTQLRAAENPQGPGAEPDTGG"
     gene            529..891
                     /locus_tag="VFO88_08195"
     CDS             529..891
                     /locus_tag="VFO88_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9545544.1"
                     /translation="MNFDISTEKLKDDAYVISLGGEVDLYTAPEFKQQLLEVIGDGAT
                     HVVVDLTKTTFIDSTTLGVLVGGVKRLRPAGGQLSLVCSDRNITKIFEITGLDKVFPI
                     HETRTEAVETIESREPAA"
     gene            857..1522
                     /locus_tag="VFO88_08200"
     CDS             857..1522
                     /locus_tag="VFO88_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9545545.1"
                     /translation="MRRSNRANRPRRLALFALLAAAALASAGCGATTVDRSDADPVAG
                     QELFVQKCGGCHTLAAAGTQGQTGPNLDDGFGFPREQGFPESTFFEVTLEKMEKPGYG
                     SVMPDFDNPDDPANFLEERDLVSIADFVASNAGTGAEAPEASADDPESLFATSCGSCH
                     VFSPAGTSGTTGPSLDDTALGVEEVARQIAEGGGGMPPFEGQLTDEQIQALAEYITSA
                     RRR"
     gene            complement(1519..2166)
                     /locus_tag="VFO88_08205"
     CDS             complement(1519..2166)
                     /locus_tag="VFO88_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545546.1"
                     /translation="MRNPLRTEAEAFSFVLVVAVLFLAVAVAAVLGGAGVALAVFGAL
                     ALGVAAGIYLRSEPKTSEPAIWERRKPDERKRILVIANETVAGTELRYEVVRRAAGDA
                     EVLVVTPALNSRLRHWTSDEDRAREEAQGRLDASLEALRDAGVDARGQVGDDDPLQAI
                     EDALRGFPADEIVLSTHPPGRSNWLERGLVENARERFACPIAHVVVDLDAAPETG"
     gene            complement(2197..2817)
                     /locus_tag="VFO88_08210"
     CDS             complement(2197..2817)
                     /locus_tag="VFO88_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9545547.1"
                     /translation="MSTVAHADTHGHDHHGPPPANMSSRVDSRLLGIYLFIASEIMLF
                     GSFFTALFFARVIVAPDAWPPEGFHLPVYLALVNTIILVTSSFTMHWALQSIKRNYRA
                     GLIAGLTLTFLLGLTFLLIQAREYTRIGFGPDENAFASTFFGLTGLHGAHVFVGLTLL
                     AFALVRSIRGHFGPDAHDHLGVEIPGIYWHFVDVMWIVVYVTVYVL"
     gene            complement(2814..3653)
                     /locus_tag="VFO88_08215"
     CDS             complement(2814..3653)
                     /locus_tag="VFO88_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545548.1"
                     /translation="MSGAPESYVRQVLVLANETVAGRSLLDAVRARAAEGPIRVTVLA
                     PQNAPRGGLVVYQDERRSVAERRLHKTLDLLHEAGIAARGAVVDPDPLQALRDGIREF
                     EPDEIIVSTHPEQRSGWLRGNLIDRARKAAGDLPLEHVVVDLEEPRERAHVLVLGSQT
                     VVGEPVLEEVRKRAAASPAEFTVVVPADPPGAEERVRRTLARLKTAGIEATGHVGDPD
                     PVAAAMNAVHDERVDEIIVSTFPQETSGWLRRDVVGRIRKGTKLPVYHIEVPESEAAE
                     VPS"
     gene            complement(3650..5578)
                     /gene="ctaD"
                     /locus_tag="VFO88_08220"
     CDS             complement(3650..5578)
                     /gene="ctaD"
                     /locus_tag="VFO88_08220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET9545549.1"
                     /translation="MSATLHAPAPRRTGIRRWTAGGWLRALWTTPLAFLLATGLVAGA
                     RAALGFDPLLAQQPYITTLMVIVPIGFLAGIGAFDYWVRYVSGRPTVPDDHASHGAHS
                     WKDYFKVNTDHKVIGTQYVVTTVVFFIAGGLLAMFMRAELAEPGMQYVDPQAYNGLFS
                     VHASLMIFLFIIPAFAGLGNYVIPLMIGAPDMAFPRLNALSFWLLPIAGLMMLSSYFI
                     GAFGAGWTNYAPLAVQQPDGNLMFQMGVQWAGASSIMTALNFLVTIITMRAPGMTFWR
                     MPLLVWANFATSTLVVIATPFVAGSQFFILFDRVLHTNFFDPGGGGYVVGYQHIFWFY
                     SHPAVYIMMLPGFGIVSEVISVFARKPIFGYRLMALSLMAILILGFSVWAHHMFVSGM
                     ADWLRVPMMVTTMVIAVPTGIKVFSWLATLWEGRIHFKTPMLFALGFVSMFVIGGLSG
                     VYLGSVPIDIHVSDTYFVVAHIHYVLFGGSLFTIYAGFYYWFPKMTGRMYNETLGKIH
                     FWLTFVGFNATFFPMHWIGTRGMPRRVADYDPMFGNWNMAISIASFALGASTLVFLYN
                     MIVSWRRGEPAPSNPWRALTLEWQVSSPPPIFNFDEIPQVVGSPYEYGVRGARHAILN
                     GKPVQEGREAAPMEETVP"
     gene            complement(5575..6600)
                     /gene="coxB"
                     /locus_tag="VFO88_08225"
     CDS             complement(5575..6600)
                     /gene="coxB"
                     /locus_tag="VFO88_08225"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9545550.1"
                     /translation="MRRRAVIQMIVIGLLAGIVTGGVAYFIPWLPDQASTQAEEIDGV
                     YWFVAIICAVVFSLVAGVSIYAGWKFRAPADDMDDGSPIHGHTGLEIWWTAVPTVLVV
                     AMSVWSAVALARAETLPDNPDEYTTIEVTAAQFAWSFTYPELERTDGELVLRVNEPVE
                     LILTANDVIHAFWVPEFRMKQDAVPGIETRTVITPTKEGSYDVICTELCGPGHAVMRA
                     GVRVLSDADYEAWVAEGEQGAGGGEGGGGEGEADGESVFAEAGCGSCHTLADAGSEAE
                     IGPDLDSVVPGQEEQQILASIVDPNAELSEGYQPNVMPGDYEDRLSEAELDALVAYLV
                     EVAGARR"
     gene            6732..>7252
                     /locus_tag="VFO88_08230"
     CDS             6732..>7252
                     /locus_tag="VFO88_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9545551.1"
                     /translation="MPRAIWTGSVTFGLVNVPVRMVSAVEERKLHFHYVHAPDSSRIG
                     YEKICKAEGKPVPDDEIVKAFEYEKGEYVFMTDEDFEAAAAESTKTIEIRDFVAADEI
                     DPIYFERTYYLAPQEGAEKVYALLVQAMERSGLVAIAKYVMRDRQNLGALRVRDGVIA
                     LERMYFGDEIRPAS"
BASE COUNT         1201 a         2465 c         2611 g          975 t
ORIGIN      
        1 cgaacgagga ggcgctccag cagctcgccg ggctctacct catccgggcg aacgaggccg
       61 acgagcgcgc cgagcgtgcg cgctccgcga gcggcgtcgc gttcttcaac gtcgagctct
      121 acgggaacga gctgagccag gccttcggcg ccgggccgtt cgtcgaggcg cagcagcagc
      181 gcacgtcgga ggcctactcg gccgccgcgc tcgaggcgca gcagtggcac gccgaggagg
      241 cggcgatctg ggagaagctc gtagacctcc agccggagga ctcgaggtac ctcctcgagc
      301 tcggccgctc gaagcagcag gcgaacgaga tccccgcggc gatcgaggcg tacgagaagt
      361 acatcgaggt cgcgcccgag gacgacccga ccgcggagca ggtcgagcag ctcctcacgc
      421 agctccgcgc cgccgagaac ccgcagggcc cgggtgcgga acccgacaca ggcggctagc
      481 cggctccgtt agggtacggc ctcagcgggg caacgaaggc gagggcggat gaacttcgac
      541 atcagcaccg agaagctcaa ggacgacgcg tacgtgatct cgctcggcgg cgaggtcgac
      601 ctctacacgg cgcccgagtt caagcagcag ctcctcgagg tgatcgggga cggcgccacg
      661 cacgtcgtcg tcgacctgac gaagacgacg ttcatcgact cgaccacgct cggcgtgctc
      721 gtcggcggcg tgaagcgcct gcgtccggcc ggcggccagc tgtcgctcgt ctgcagcgac
      781 cggaacatca cgaagatctt cgagatcacc ggtctcgaca aggtcttccc gatccacgag
      841 acgcgcaccg aggccgttga gacgatcgaa tcgcgcgaac cggccgcgta ggctcgccct
      901 cttcgcactg ctcgcggccg ccgcgctcgc ctcggcgggc tgcggcgcga cgacggtcga
      961 ccgcagcgac gccgacccgg tcgccgggca ggagctcttc gtgcagaagt gcggcggctg
     1021 ccacacgctc gccgcggcgg ggacgcaggg acagacgggg ccgaacctcg acgacggctt
     1081 cgggttcccg cgcgagcagg gattccccga gagcacgttc ttcgaggtga cgctcgagaa
     1141 gatggagaag cccggctacg gctccgtcat gccggacttc gacaacccgg acgatccggc
     1201 caacttcctc gaggagcgcg acctcgtcag cattgccgac ttcgtcgcgt cgaacgcggg
     1261 caccggcgcc gaggcgcccg aggcgtccgc ggacgatccg gagagcctct tcgccacctc
     1321 gtgcggaagc tgccacgtct tctcgcccgc cgggacgagc gggacgaccg ggccgagcct
     1381 cgacgacacg gcgctcggcg tcgaggaggt cgcgcgccag atcgcggagg gcggcggcgg
     1441 gatgccgccg ttcgagggtc agctcacgga cgagcagatc caggcgcttg ccgagtacat
     1501 cacctcggcg cgcaggcgct aaccggtctc cggcgccgcg tcgagatcga cgacgacgtg
     1561 cgcgatcggg cacgcgaagc gctcgcgggc gttctcgacg aggccgcgct cgagccagtt
     1621 cgagcggccc ggcggatgcg tcgagaggac gatctcgtcc gccgggaagc cgcgcagcgc
     1681 gtcctcgatc gcctggagcg gatcgtcgtc cccgacctgc ccgcgcgcgt cgacgccggc
     1741 gtcgcggagc gcctcgagcg acgcgtcgag ccgcccctgc gcctcctcgc gggcgcggtc
     1801 ctcgtccgag gtccagtggc gcagccgcga gttcagggcg ggcgtgacga cgagcacctc
     1861 ggcgtcgccg gcggcgcgcc ggacgacctc gtagcggagc tccgtcccgg cgaccgtctc
     1921 gttcgcgatc acgaggatgc gcttccgctc gtccggcttg cgccgctccc agatcgccgg
     1981 ctcgctcgtc ttcggctcgc tgcggagata gatcccggcc gcgacgccga gcgcgagcgc
     2041 cccgaacacc gcgagcgcga ccccggcacc gcccagcacc gccgcgaccg cgaccgcgag
     2101 gaagagcacg gcgacgacga gcacgaagct gaacgcctct gcctcggtgc gtaacgggtt
     2161 gcgcatcgcg cctcccttcg tagcggcggc ggcggtctac aggacgtaga ccgtgacgta
     2221 cacgactatc cacattacgt ccacgaagtg ccagtagatg cccgggatct cgacgccgag
     2281 gtgatcgtgg gcgtcgggcc cgaagtggcc gcggatcgag cggacgaggg cgaacgcgag
     2341 caacgtgagg ccgacgaaca cgtgcgcgcc gtgcagcccg gtcaggccga agaacgtcga
     2401 tgcgaacgcg ttctcgtcgg ggccgaagcc gatccgcgtg tactcgcgcg cctggatcag
     2461 gaggaacgtg agcccgagga ggaacgtcag cgtgaggccg gcgatgagcc cggcgcggta
     2521 gttgcgcttg atcgactgga gggcccagtg catcgtgaag ctcgaggtca ccaggatgat
     2581 cgtgttcacg agcgcgaggt agaccgggag gtggaagccc tccggcggcc acgcgtccgg
     2641 ggcgacgatc acgcgcgcga agaagagcgc ggtgaagaac gacccgaaga gcatgatctc
     2701 cgacgcgatg aagaggtaga tcccgagcag gcgcgagtcg acgcgcgagc tcatgttcgc
     2761 gggcggcggc ccgtggtggt cgtgcccgtg cgtgtcggcg tgcgcgaccg tgctcacgac
     2821 ggcacctcgg cggcctcgct ctccggcacc tcgatgtggt agacgggcag cttcgtgccc
     2881 ttgcggatgc gcccgacgac gtcgcggcgc agccagccgg aggtctcctg cgggaaggtc
     2941 gacacgatga tctcgtcgac gcgctcgtcg tgcaccgcgt tcatcgccgc ggcgaccggg
     3001 tcggggtcgc cgacgtggcc ggtcgcctcg atgcccgcgg tcttcaggcg ggcgagcgtg
     3061 cgccggacgc gctcctcggc gccgggcggg tcggccggga cgacgacggt gaactcggcc
     3121 ggggaggcgg cggcgcgctt gcggacctcc tcgagcaccg gctcgccgac gaccgtctgg
     3181 ctgccgagca cgaggacgtg cgcgcgctcg cggggctcct cgaggtcgac gacgacgtgc
     3241 tcgagcggca ggtcgccggc cgccttgcgc gcgcggtcga tgaggttccc gcgcagccag
     3301 ccggagcgct gctccgggtg cgtcgagacg atgatctcgt ccggctcgaa ctcgcggatc
     3361 ccgtcgcgga gcgcctggag cgggtccggg tcgacgaccg cgccgcgggc cgcgatgccg
     3421 gcctcgtgga gcaggtcgag cgtcttgtgc agccggcgct cggcgaccga gcggcgctcg
     3481 tcctggtaga cgacgagccc gccgcgcggg gcgttctgcg gcgcgaggac ggtgacccgg
     3541 atcggcccct ccgcggcgcg ggcccggacc gcgtcgagga gcgagcgccc ggcgaccgtc
     3601 tcgttcgcga gcacgagcac ctgccggacg tagctctccg gcgccccgct cacggcacgg
     3661 tctcctccat cggcgcggcc tcgcggccct cctgcaccgg cttcccgttc aggatcgcgt
     3721 gccgggcgcc gcggacgccg tactcgtacg gcgagccgac cacctgcggg atctcgtcga
     3781 agttgaagat cggcggcggc gaggagacct gccactcgag cgtgagggcc cgccaggggt
     3841 tcgagggcgc cggctcgccg cgccgccagc tgacgatcat gttgtagagg aagacgagcg
     3901 tcgaggcccc gagcgcgaac gacgcgatcg agatggccat gttccagttc ccgaacatcg
     3961 ggtcgtagtc ggcgacccgg cgcggcatcc ccctcgtccc gatccagtgc atggggaaga
     4021 aggtcgcgtt gaagccgacg aacgtcagcc agaagtggat cttgccgagc gtctcgttgt
     4081 acatccggcc ggtcatcttc gggaaccagt agtagaagcc cgcgtagatc gtgaagagcg
     4141 agccgccgaa gaggacgtag tggatgtggg cgaccacgaa gtacgtgtcg ctcacgtgga
     4201 tgtcgatcgg caccgagccg aggtagacgc ccgacagccc gccgatcacg aacatcgaca
     4261 cgaagccgag cgcgaacagc atgggcgtct tgaagtggat gcgcccctcc cagagcgtgg
     4321 cgagccagga gaagaccttg atcccggtcg gaacggcgat caccatcgtc gtgaccatca
     4381 tcgggacgcg cagccagtcg gccatgccgc tcacgaacat gtggtgcgcc cagaccgaga
     4441 acccgaggat gaggatcgcc atgagcgaga gggccatcag ccggtagccg aagatcggtt
     4501 tccgcgcaaa gacggagatg acctcggaga cgatcccgaa cccgggcagc atcatgatgt
     4561 agacggccgg gtgcgagtag aaccagaaga tgtgctggta gccgacgacg taccccccgc
     4621 cgccggggtc gaagaagttc gtgtggagga cgcggtcgaa gaggatgaag aactgcgacc
     4681 ccgcgacgaa cggcgtcgcg atcacgacga gcgtcgaggt cgcgaagttc gcccagacga
     4741 ggagcggcat gcgccagaac gtcatccccg gcgcgcgcat cgtgatgatc gtgaccagga
     4801 agttgagcgc ggtcatgatc gacgacgcgc cggcccactg gacgcccatc tggaacatga
     4861 ggttcccgtc cggctgctgc acggcgagcg gtgcgtagtt cgtccagccg gccccgaacg
     4921 cgccgatgaa gtagctcgag agcatcatga ggcccgcgat cgggaggagc cagaacgaga
     4981 gggcgttcag gcgcgggaac gccatgtccg gcgcgccgat catgagcggg atgacgtagt
     5041 tcccgagccc cgcgaacgcc gggatgatga agaggaagat catcagcgac gcgtggacgc
     5101 tgaagaggcc gttgtaggcc tgcgggtcga cgtactgcat ccccggctcg gcgagctctg
     5161 cgcgcatgaa catcgcgagg aggccgccgg cgatgaagaa gacgaccgtg gtgacgacgt
     5221 actgcgtgcc gatcaccttg tggtcggtgt tgaccttgaa gtagtccttc caggagtggg
     5281 ccccgtggct cgcgtggtcg tccgggaccg tcgggcggcc ggagacgtag cgcacccagt
     5341 agtcgaaggc tccgatcccg gcgaggaagc cgatcgggac gatcaccatg agcgtcgtga
     5401 tgtacggctg ctgggcgagg agcgggtcga agccgagcgc ggcgcgggcg cccgccacga
     5461 ggcccgtggc gaggaggaac gcgagcggcg tcgtccagag ggcgcggagc cagccgcccg
     5521 ccgtccagcg gcggattccg gtccggcgcg gcgccggcgc gtgcagcgtc gcgctcaccg
     5581 gcgcgccccc gcgacctcga cgaggtaggc gacgagcgcg tcgagctcgg cctccgacag
     5641 gcggtcctcg tagtccccgg gcatgacgtt cggctggtag ccctcgctga gctcggcgtt
     5701 cgggtcgacg atcgacgcca ggatctgctg ctcctcctgg cccgggacga cactatcgag
     5761 atcgggaccg atctcggcct cgctgccggc gtcggccagc gtgtggcagc ttccgcagcc
     5821 ggcctcggcg aagaccgact cgccgtccgc ctcgccctcg ccgccgccgc cctcgccccc
     5881 gcctgcgccc tgctcgccct cggcgaccca tgcctcgtag tccgcgtccg agagcacgcg
     5941 cacgcccgcg cgcatgaccg cgtggcccgg gccgcagagc tcggtgcaga tcacgtcgta
     6001 cgacccctcc ttcgtcggcg tgatcacggt gcgcgtctcg atgcccggca ccgcgtcctg
     6061 cttcatgcgg aactccggca cccagaaggc gtggatgacg tcgttggccg tcaggatcaa
     6121 ctcgacgggc tcgttcacgc ggagcacgag ctcgccgtcc gtccgctcga gctccgggta
     6181 cgtgaagctc caggcgaact gcgccgcggt cacctcgatc gtcgtgtact cgtcggggtt
     6241 gtccggaagc gtctcggcgc gggcgagcgc gacggcgctc cagacggaca tggcgacgac
     6301 gagcacggtc gggaccgcgg tccaccagat ctcgagcccg gtgtgaccgt ggatcggcga
     6361 gccgtcgtcc atgtcgtccg ccggcgcgcg gaacttccag cccgcgtaga tcgagacgcc
     6421 cgcgacgagc gagaagacga ccgcgcagat gatcgccacg aaccagtaga cgccgtcgat
     6481 ctcctccgcc tgcgtggacg cctggtcggg gagccacggg atgaagtagg cgaccccgcc
     6541 ggtgacgatc ccggcgagga ggccgatcac gatcatctgg atgaccgcgc gtcttctcat
     6601 tcggaaaaag aggcgaggcg tccggcccgg cccgcgagaa atctaaccca ccgggaggag
     6661 caccggagcg cccaccggag gcggcctccg agtacgtttc gatggtcaca gttcgcggga
     6721 caacgggaag cgtgccgcgc gcgatctgga ccggctccgt cacgttcggg ctcgtcaacg
     6781 tgcccgtccg gatggtgagc gccgtggagg agcgcaagct ccacttccac tacgtccacg
     6841 cccccgactc gagccggatc ggctacgaga agatctgcaa ggccgagggc aagcccgtcc
     6901 cggacgacga gatcgtcaag gcgttcgagt acgagaaggg cgagtacgtc ttcatgacgg
     6961 acgaggactt cgaggccgcg gccgccgaga gcacgaagac gatcgagata cgcgacttcg
     7021 tcgcggcgga cgagatcgac ccgatctact tcgagcggac gtactacctc gcgccgcagg
     7081 agggcgcgga gaaggtctac gcgctcctcg tccaggcgat ggagcgctcc ggcctcgtgg
     7141 cgatcgcgaa gtacgtcatg cgcgaccgcc agaacctcgg cgcgctgcgc gtccgcgacg
     7201 gcgtgatcgc gctcgagcgg atgtacttcg gcgacgagat ccggcccgcg tc
//
LOCUS       DASTFC010000278         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1061663,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000278 DASTFC010000000
VERSION     DASTFC010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061663"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1376
                     /locus_tag="VFO88_08235"
     CDS             162..1376
                     /locus_tag="VFO88_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9545552.1"
                     /translation="MTRVRIPPTLRPDVGGAREVEASGSTLREVLDDLVARHPALAGR
                     LFENGDVASFVNVYVNSEDARTLDGLDTPTPEGSTVILLPALAGGEHVVAPARSLLDT
                     IGRTPLVELSRLAPHEGIRLYAKLEGSNPTGSTKDRVARAMVEAAEERGELEPGRELI
                     EPTSGNTGISLALVARLKGYRLTCVVPENATEERLRLLELYGARIVTSPASQGSNGAV
                     RQALRMAESDSRYVMLNQYGNDANPRAHYEGTGAEIVEALPRVDALVAGLGTGGTLMG
                     AGERLREAFPGVTIAAAEPLQGDLVYGLRSLADGYVPPILDVSRLDRKVLVTNEESVE
                     GVRALLDEEGIFAGVSSGAVLHVARKIAGELDEGVVVCVLADGGWKYLSAPFWAERNG
                     GVGPSMEDRLWW"
     gene            1373..1747
                     /locus_tag="VFO88_08240"
     CDS             1373..1747
                     /locus_tag="VFO88_08240"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET9545553.1"
                     /translation="MIPPVVRSALVEHAEAEKPNEACGLVAVRDGEAERYIPARNGAA
                     SPYRFELEADPEAWFLEDEGYELAVFHSHLASPPRPSRTDVENIGLWEGRPYLILALH
                     TGELAGWRIVDGRSEPLELSAA"
     gene            1763..2281
                     /locus_tag="VFO88_08245"
     CDS             1763..2281
                     /locus_tag="VFO88_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545554.1"
                     /translation="MRLVTLCVLALLLAACGSSDADPAAEPDPAPATTTAPETTTGQS
                     GGPSEEPPVAEEGGPPPAWIETPAASAWMSFGRYCWGDVCLESVQVTCDDSSVPQLDL
                     AAGDTVRFHLDFEPDTAELTLGADGNRPEVVTLEPARTMEWQGDYDGALWLAVTSGQD
                     EVAYVACASAVS"
     gene            complement(2274..2630)
                     /locus_tag="VFO88_08250"
     CDS             complement(2274..2630)
                     /locus_tag="VFO88_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545555.1"
                     /translation="MIRLTLVALVAAALLAGTAAPADAARLTGTVQGERTITLKKRNG
                     TVVRTLAAGRHTFVIRDRSSIHNFVLARGQNRIRGTGISFVGTETWQVRIRRGAIYRY
                     FCQTHTAQMSKTFRVS"
     gene            2835..3965
                     /locus_tag="VFO88_08255"
     CDS             2835..3965
                     /locus_tag="VFO88_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9545556.1"
                     /translation="MSTNGTSLQTARARYTETGVRWGFIWALWCAVLWGAWYVPGYAL
                     FYEEPFVSNQDTTAGLLKVAAAITVLNAIFVLLAMFIWMAVLGKVRDYGRTLAQSRNV
                     SRWLWAGAVFGGPIAIYGTYLALAYVGAAFGAVAALLYPIVGALLARVWYHERITRRA
                     ALGIIVVVIGGVFIFTPGLLDEIRGTGAGDNAWLGYLGGIMSFVGWGIEGAIAGRALD
                     VTDPDVALPIRFTAENVYWLLIIVPIASIFVGGDLYSLMWDGLTNGIVVLWLALAGLT
                     FGFCYVSWYKAFPLIGVGRGQAFADLYGFFALVWLTIFTLAAPSWQFWVGGLIAILGG
                     FIMYTERRDVLEVIRAVPGGHEDEGPIAEVHGGAGDLTGIGR"
     gene            3971..4288
                     /locus_tag="VFO88_08260"
     CDS             3971..4288
                     /locus_tag="VFO88_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545557.1"
                     /translation="MAQLHIKGRILQLLEERGPLWDYEIFELIAAEYGEVEGQYWYDT
                     LRLVLADLYSSGLTKSIEEKLDAGKSFGVEKILFRFDLTNFGRERMRHTGLSTGRRQE
                     VAA"
BASE COUNT          584 a         1521 c         1563 g          661 t
ORIGIN      
        1 gacacgctga ccgggcgcct gctcctcttc gacgcgctcg agacgacgtt caccgaggtg
       61 gcgctccgcc gcgacccgga ctgcccggtc tgcggcgaca gcccgacgat caccgagtac
      121 atcgactacg tccagttctg cgcgggcccc cgcgcgcacg catgacgcgc gtcagaattc
      181 cgcccacgct gcgtcccgac gtcggcgggg cgcgggaggt cgaggcctcc ggctcgacgc
      241 ttcgcgaggt gctcgacgac ctcgtcgccc ggcaccccgc gctcgcgggc cggctcttcg
      301 agaacggcga cgtggcctcc ttcgtgaacg tgtacgtgaa cagcgaggac gcccggacgc
      361 tcgacgggct cgacacgccg acgcccgagg gctcgaccgt gatcctcctc cccgcgctcg
      421 cgggcggcga gcacgtcgtc gcgccggcca ggtcgctcct cgacacgatc gggcgcacgc
      481 cgctcgtcga gctgtcgcgc ctcgcgcccc acgagggcat caggctgtac gcgaagctcg
      541 agggctcgaa cccgacgggc tcgacgaagg accgcgtcgc gcgggcgatg gtcgaggcgg
      601 ccgaggagcg cggcgagctc gagcccggcc gcgagctcat cgagccgacg agcgggaaca
      661 ccggcatctc cctcgcgctc gtcgcgcggc tcaagggcta ccggctcacg tgcgtcgtcc
      721 cggagaacgc gaccgaggag cggctccggc tgctcgagct ctacggagcg cggatcgtca
      781 cgtcgcccgc ctcgcagggg tcgaacggcg cggtgcgcca ggcgctccgg atggcggagt
      841 ccgactcgcg ctacgtgatg ctgaaccagt acggaaacga cgcgaacccg cgcgcgcact
      901 atgagggcac cggcgccgag atcgtcgagg cgctcccgcg cgtggacgcg ctcgtcgcgg
      961 ggctcgggac gggcggcacg ctgatgggcg ccggcgagcg gctgcgcgag gccttccccg
     1021 gcgtcacgat cgcggccgcg gagccgctcc agggcgacct cgtctacggc ctgcgctcgc
     1081 tcgcggacgg gtacgtcccg ccgatcctcg acgtgtcgcg cctcgaccgg aaggtgctcg
     1141 tcacgaacga ggagtcggtc gagggcgtcc gcgcgctgct cgacgaggag gggatcttcg
     1201 cgggcgtctc gtcgggcgcc gtgctccacg tcgcccgcaa gatcgccgga gagctcgacg
     1261 agggtgtcgt cgtctgcgtg ctcgcggacg gcggctggaa gtacctgagc gcgcccttct
     1321 gggccgagag gaacggcggc gtcgggccgt ccatggagga ccggctctgg tggtgatccc
     1381 gcccgtggtg cgctcggcgc tcgtcgagca cgccgaggcg gaaaagccga acgaggcctg
     1441 cgggctcgtc gccgtgcgcg acggcgaggc cgagcgctac atcccggcgc ggaacggcgc
     1501 cgcgagcccg taccggttcg agctcgaggc cgatccggag gcctggttcc tcgaggacga
     1561 gggctacgag ctcgccgtct tccactccca tctcgcctcg ccgccccgcc cgtcgcggac
     1621 ggacgtcgag aacatcggcc tctgggaggg ccggccgtac ctcatcctcg cgctccacac
     1681 cggcgagctc gccggctggc ggatcgtcga cgggcgctcg gagccgctgg aactttcggc
     1741 ggcctagctc gtcggtaagg gtatgaggct cgtgaccctc tgcgtgctcg cgctcctcct
     1801 cgccgcctgc gggtcgtcgg acgccgaccc ggcggccgag ccggacccgg cgcccgccac
     1861 gacgacggcg ccggagacga cgaccggaca gtcgggcggc ccctccgagg agccgccggt
     1921 cgcggaggag ggcgggccgc cgcccgcctg gatcgagacg ccggccgcct ccgcctggat
     1981 gtcgttcggg cgctactgct ggggcgacgt ctgtctcgag agcgtccagg tgacgtgcga
     2041 cgactcgtcc gtgccgcagc tcgacctcgc ggccggcgac accgtgcgct tccacctcga
     2101 cttcgagccg gacacggccg agctgacgct cggcgccgac ggaaaccggc ccgaggtcgt
     2161 caccctcgag cccgcccgga cgatggaatg gcagggcgac tacgacggcg ccctctggct
     2221 cgcggtcacg agcgggcagg acgaggtcgc ctacgtggcg tgcgccagcg cggtcagctg
     2281 acgcggaacg tcttcgacat ctgcgccgtg tgcgtctggc agaagtagcg gtagatcgcg
     2341 ccgcgccgga tccggacctg ccaggtctcg gtgccgacga acgagatgcc ggttccccgg
     2401 atgcggttct ggccgcgcgc taggacgaag ttgtggatgc tcgagcggtc gcggatcacg
     2461 aacgtgtgcc gccccgccgc gagggtgcgg acgacggtgc cgttgcgctt cttcagcgtg
     2521 atggtgcgct ccccctgaac cgtgcccgtc agccgcgcgg catccgcggg cgcggccgtg
     2581 ccggcaagga gcgcggcggc gacgagagcg accagggtca gccggatcac gcttctcgat
     2641 acgaccgctg cgggccgggc ggattgcgct ccgcacgctt caggccgcgc cggcgcgcat
     2701 ttcgtagcgg ttgccacgag aaatctgcgc aacggtcggc gaatttcggc gcgtcccttg
     2761 cgtctcgtca agccggggcg cgataatcgc gccttcgccg ctactggcgc aacatccact
     2821 ctaggaggtg ttggatgtcc acgaacggga cgtcgctgca gacggcccgg gcgcgctaca
     2881 cggagaccgg cgtccgctgg gggttcatct gggctctctg gtgcgcggtc ctgtggggcg
     2941 cctggtacgt gcccggctac gccctcttct acgaggagcc gttcgtgtct aaccaggaca
     3001 cgacggcagg cctgctcaag gtggccgccg ccatcaccgt gctgaacgcg atcttcgtcc
     3061 tcctcgccat gttcatctgg atggcggttc tcggaaaggt cagggactac ggaaggacgc
     3121 tcgcgcagtc gcggaacgtc tcgcgctggc tctgggccgg cgcggtcttc ggcggcccga
     3181 tcgcgatcta cgggacgtat ctcgcgctcg cctacgtcgg cgcggcgttc ggcgcggtgg
     3241 ccgcgcttct ctacccgatc gtgggcgccc tgctcgcgcg ggtctggtac cacgagcgga
     3301 tcacgcggcg cgcggcgctc gggatcatcg tggtggtcat cggcggcgtg ttcatcttca
     3361 cgccggggct cctcgacgag atccgcggga ccggcgccgg cgacaacgcc tggctcggct
     3421 acctcggcgg gatcatgtcg ttcgtcggct ggggcatcga gggcgcgatc gccggccggg
     3481 cgctcgacgt gacggatccg gacgtggcgc tccccatccg cttcacggcc gagaacgtct
     3541 actggctcct gatcatcgtc ccgatcgcct cgatcttcgt cggcggcgac ctctactcgc
     3601 tcatgtggga cgggctcacg aacggcatcg tcgtcctgtg gctcgccctg gcagggctga
     3661 cgttcgggtt ctgctacgtg tcctggtaca aagccttccc cttgatcggg gtggggcgag
     3721 gccaggcgtt cgcggacctc tacgggttct tcgcgctcgt ctggctgacg atcttcacgc
     3781 tcgccgcgcc gtcctggcag ttctgggtcg gcggcctcat cgccatcctc ggcgggttca
     3841 tcatgtacac cgagcgtcgc gacgtcctcg aggtcatccg ggcggtgccc ggcgggcacg
     3901 aggacgaggg cccgatcgcc gaggtccacg gcggcgccgg cgacctgacc ggcattggga
     3961 ggtgacgggg atggcacagc tccacatcaa gggccgcatc ctccagctcc tcgaggagcg
     4021 cgggccgctc tgggactacg agatcttcga gctcatcgcg gccgagtacg gcgaggtcga
     4081 agggcagtac tggtacgaca cgctcaggct cgtcctggcc gacctctatt cgagcgggct
     4141 cacgaagtcg atcgaggaga agctcgacgc cggcaagagc ttcggcgtcg agaagatcct
     4201 cttccgcttc gacctcacga acttcggccg tgagcggatg cggcacacgg gcctctcgac
     4261 cggacgcagg caggaggtgg cggcatgagc acgcttctcg cggtcagcga gttctggggc
     4321 ggggacggc
//
LOCUS       DASTFC010000279         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_188128,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000279 DASTFC010000000
VERSION     DASTFC010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188128"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1935
                     /locus_tag="VFO88_08265"
     CDS             <1..1935
                     /locus_tag="VFO88_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9545558.1"
                     /translation="VVETHGTRMAGILVGSGGPAGLEGVAPGAQLLPIRILGWERAAD
                     GEYALLGHGDDLLAGLERAVDPNGDGSTEDALPVALAAVVEPYASFPDSPESRAVEGA
                     ARLGTLVVAAAGNDGRAGRGFGTVGAPGGAPAALTVGASDSRAEAIESSVRLTVDGDE
                     ILAEALPVLGSLPPGGSLPVAALGGPSLAKPARAADDVADGSVLADFFDARGVSRVAG
                     RAALVPGGGPLDERVRNAAAAGASVVLVAGGDAAAGSVDLDETTAVPVVSLPPEAGRE
                     AFETLAEGGAVSVAFDEARLVPNETAGEVAPFSSGGMAFGGHVKPDVVAPGVGIATTD
                     AGTDADGSARYATATGSSVSAALVAGSAAALLQARPGLSAAELRSLVVGSARQIVLDG
                     ESVPVTVQGAGIVDPAAAAAAELVVEPVSLAFGRAGRDEWQVKQTVRIRNLATRALEL
                     GFGLSRDTSEGPELAFSAVPASLALEAGASGEVTLLASGTGPLEGQAGGAFVVQPEGS
                     RAVRVPWAVSFRGESPPALLTEVRLSEEQFRASDASPAVLTFVAGGVVAEADGQAIEP
                     VQLLTAELWTAKGRRLGVLSRMHDLLPGRYAVGVTGRDGNGKALRPGRYVLRLVAEPP
                     AGDVGAQATVRDVPFRLLPR"
BASE COUNT          212 a          808 c          811 g          258 t
ORIGIN      
        1 gtcgtcgaga cccacgggac gcgcatggcc ggcatcctcg tcggaagcgg cgggcccgcc
       61 gggctcgagg gcgtcgcgcc gggcgcgcag ctcctgccga tcaggatcct cggctgggag
      121 cgcgccgccg acggcgagta cgcgctcctc ggccacggcg acgacctcct ggcggggctc
      181 gagcgcgcgg tcgacccgaa cggcgacggc tccacggagg atgcgctccc ggtcgcgctc
      241 gcggcggtcg tcgagccgta cgcgtccttc ccggacagcc cggagtcgcg cgcggtggag
      301 ggtgccgcgc ggctcggcac gctcgtcgtg gcggcggccg gaaacgacgg gcgcgcgggc
      361 cgggggttcg gcacggtcgg cgcgccgggc ggtgccccgg ccgcgctcac ggtcggcgcg
      421 tccgactcgc gggccgaggc gatcgagagc tccgtccgcc tgaccgtcga cggggacgag
      481 atcctcgcgg aggcccttcc ggtgctcggc agcctcccgc ccggcggttc cctgccggtc
      541 gcggcgctcg gcggcccctc gcttgcgaag cccgcgcgcg cggcggacga cgtcgccgac
      601 ggctcggtgc tcgcggactt cttcgacgcc cgtggcgtga gccgggtcgc cggccgcgcg
      661 gcgctcgttc ccggcggcgg tccgctggac gagcgcgtgc gcaacgcggc cgcggcgggc
      721 gcgagcgtcg tgctcgtggc cggcggcgac gcggcggcgg gctcggtcga cctcgacgag
      781 acgaccgccg tcccggtcgt ctcgctgccg cccgaggccg gacgcgaggc gttcgagacg
      841 ctcgccgagg gcggagcggt gtcggtcgcc ttcgacgagg cgcggctcgt cccgaacgag
      901 acggccggcg aggtcgcgcc cttctcctcg ggcgggatgg ccttcggcgg ccacgtgaag
      961 ccggacgtcg tcgcgcccgg cgtcgggatc gcgacgacgg acgcgggaac cgacgccgac
     1021 ggctccgccc gctacgcgac cgcgaccggc tcgagcgtct cggccgcgct cgtcgcgggc
     1081 tcggcggcgg ccctgctcca ggcgcgcccc ggcctctccg cggcggagct gcgcagcctc
     1141 gtcgtcggaa gcgcccgcca gatcgtcctc gacggggaga gcgtccccgt caccgtccag
     1201 ggagccggga tcgtcgaccc cgccgccgcg gccgccgcgg agctcgtcgt cgagccggtc
     1261 tcgctcgcct tcgggcgcgc gggccgcgac gagtggcagg tgaagcagac cgtccggatc
     1321 cgcaacctcg ccacgcgcgc gctcgagctc ggcttcggcc tctcgcgcga cacgtccgag
     1381 ggccccgagc tcgcgttctc ggcggttccc gcgtcgctcg cgctcgaggc cggcgcgtcg
     1441 ggcgaggtga cgctcctcgc ctccggcacc ggcccgctcg aggggcaggc cggcggggcg
     1501 ttcgtcgtcc agccggaggg ctcgcgcgcg gtgcgcgtcc cgtgggcggt gagcttccgc
     1561 ggcgagtcgc cgcccgcgct cctcaccgag gtcaggctct ccgaggagca gttccgcgcc
     1621 tccgacgcct cgcccgccgt gctcacgttc gtcgcgggcg gcgtcgtcgc cgaggccgac
     1681 ggccaggcga tcgagcccgt ccagctcctg accgccgagc tctggacggc gaagggccgc
     1741 cggctcggcg tcctctcgcg catgcacgac ctcctcccgg gccgctacgc ggtcggcgtc
     1801 accggacgcg acggaaacgg gaaggcgctg cggccgggcc gttacgtgct ccggctcgtc
     1861 gccgagccgc cggccggcga cgtgggtgcg caggcgaccg tgcgggacgt gccgtttaga
     1921 ctcctgccgc ggtgaccgcg ctcgacacac ccgcgccgtc cgtccaggag aacgcctggg
     1981 agatggcacg ggagcagctc cgccgcacgg cgctgctcgc ggagctccac gagagcctct
     2041 tcaacgtcct cggcgactgc aagaaggcgg tcgaggtctc cgtcccgac
//
LOCUS       DASTFC010000280         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1094726,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000280 DASTFC010000000
VERSION     DASTFC010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094726"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFO88_08270"
     CDS             complement(<1..444)
                     /locus_tag="VFO88_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545559.1"
                     /translation="MRVWLAAVLALLVATTAAAVAAVFVGRFDAEFEGRAGNIAVGYS
                     VDAAGRISRAADVEAVQGVTTRASNELPIALFAFAEDGTLLTSARSRGIPLELVPDSG
                     TALDEALAGRRYVETAGDGAGTVVALPVRSGPASAVVAFASHPELE"
     gene            563..1702
                     /locus_tag="VFO88_08275"
     CDS             563..1702
                     /locus_tag="VFO88_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9545560.1"
                     /translation="MPGIGVDRMGDAADAAGDPGLLRLENLDTDVRPPEAALAATRAA
                     VDDDAANSYLPFPGHAGLRRAAAAHVSALAGVEYDWRASCVVAAGGLNGILNVLLAIL
                     DPGDEVVLAEPVYVGLLNRVRVAGGVPRLARLVPGSGGWRLDLDSLRAAVGPRTRAIL
                     AVSPSMPTGAVHDGEEWEAIAELCREHDLWLVDDAAFERLLFDGREVLHPASLHGMTE
                     RTIVVGSASKELRMIGWRVGWVVGPPDVIADVGLVGISNVVCQVGIAQAAVEAALTGD
                     DGVPAFVAELERRRDLILRELDGLPVLAPHGGWSLLLDTGALGLSGAEASARLFTDGR
                     VAATPMENWGSEDTARYLRFVYANEPVERLAGLGERVRRALATDG"
     gene            1695..>2261
                     /locus_tag="VFO88_08280"
     CDS             1695..>2261
                     /locus_tag="VFO88_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9545561.1"
                     /translation="MADALLVRGGTVVDGTGGPARRADVLLRDGRVEALGALAPSPAA
                     RELDAEGLVVAPGFVDLHTHADFTLLAFPDAQSAVRQGVTTVVVGNCGGGVAPCSPEH
                     DFRRTAFAYSSDWGVEVTWEGFGEYLETLGGKGVNVAALVPHGATRNAVMGLDARPPG
                     PAELDRMLGLAREGLEAGAIGVSTGLEYQ"
BASE COUNT          255 a          867 c          858 g          281 t
ORIGIN      
        1 ctcgagctcc gggtggctcg cgaacgcgac gaccgcgctc gccggcccgc tccgcaccgg
       61 gagggcgacg accgttcccg cgccgtcgcc ggcggtctct acgtaccgcc gcccggcgag
      121 cgcctcgtcg agcgccgttc cgctgtccgg gacgagctcg agcgggatcc cgcgcgagcg
      181 cgcggaggtc aggagtgtcc cgtcctcggc gaacgcgaag agcgcgatcg gcagctcgtt
      241 cgaggcgcgc gtcgtgacgc cctggacggc ctcgacgtcg gcggcgcgcg agatccggcc
      301 ggccgcgtcg acgctgtagc cgacggcgat gtttccggcg cggccctcga actccgcgtc
      361 gaaccggccg acgaacacgg ccgcgacggc ggccgcggtg gtcgccacga ggagcgcaag
      421 gaccgcggcg agccagacgc gcatgctgag cgttcctggt acgcccatga ggagtccatg
      481 ccccctcgcg gcccaacgta ccccggggag ccgaccggcg taggatccgc gcatgcgccg
      541 ccggccctct cgcctcgacg gcatgccggg gataggcgtc gaccggatgg gcgacgccgc
      601 cgacgccgca ggcgatcccg ggctgctccg gctggagaac ctcgacacgg acgtccgccc
      661 gcccgaggcg gcgctcgccg ccacgcgggc cgcggtcgac gacgacgcag cgaacagcta
      721 cctgcccttc cccgggcacg cggggctccg ccgggcggcg gccgcgcacg tctccgcgct
      781 cgccggggtc gagtacgact ggcgcgcctc gtgcgtcgtc gcggcgggcg ggctgaacgg
      841 gatcctgaac gtcctcctcg ccatcctcga cccgggcgac gaggtcgtcc tcgccgagcc
      901 cgtctacgtc gggctcctga accgggtccg ggtcgccggc ggcgtcccgc ggctcgcgcg
      961 cctcgtgccg ggctcgggcg gctggcgcct cgacctcgac tcgctccgcg ccgcggtcgg
     1021 cccacgcacg cgcgcgatcc tcgccgtgag cccctcgatg ccgaccggcg ccgtccacga
     1081 cggcgaggag tgggaggcga tcgccgagct ctgccgcgag cacgacctct ggctcgtcga
     1141 cgacgcggcg ttcgagcggc tgctcttcga cgggcgcgag gtcctccacc ccgcctcgct
     1201 ccacgggatg accgagcgga cgatcgtcgt cggctcggcc tcgaaggagc tccgcatgat
     1261 cggctggcgc gtcggctggg tcgtcggccc gcccgacgtg atcgcggacg tcggcctcgt
     1321 cgggatctcg aacgtcgtct gccaggtcgg gatcgcgcag gccgccgtcg aggcggcgct
     1381 cacaggcgac gacggggtgc cggccttcgt cgcggagctc gagcggaggc gcgacctcat
     1441 cctgcgggag ctcgacgggc tcccggtgct cgcgccccac ggcggctggt cgctcctcct
     1501 cgacaccggc gcgctcgggc tctcgggcgc cgaggcgagc gcgcggctct tcacggacgg
     1561 ccgcgtcgcg gcgacgccga tggagaactg ggggagcgag gacacggcgc gctacctccg
     1621 gttcgtctac gcgaacgagc ccgtcgagcg gctcgccggg ctcggcgagc gggtccggcg
     1681 cgcgctcgcg acggatggct gacgcgcttc tcgttcgcgg cgggacggtc gtggacggga
     1741 cgggcgggcc ggcgcggcgc gccgacgtcc tcctccgcga cggccgcgtg gaggcgctcg
     1801 gcgcgctcgc gccctcgcca gccgcgcgcg agctcgacgc ggaggggctc gtggtcgccc
     1861 ccggcttcgt cgacctgcac acgcacgccg acttcacgct cctcgccttc ccggacgccc
     1921 agagcgccgt ccgccagggc gtcacgacgg tcgtcgtcgg gaactgcggc ggcggcgtcg
     1981 cgccctgctc gcccgagcac gacttccgcc gcacggcgtt cgcgtacagc tccgactggg
     2041 gcgtcgaggt cacgtgggag ggcttcgggg agtacctcga gacgctcggc gggaagggcg
     2101 tgaacgtcgc cgcgctcgtc ccccacggcg cgacgcggaa cgcggtcatg gggctcgacg
     2161 cccgcccgcc cgggccggcc gagctcgacc ggatgctcgg gctcgcgcgc gaggggctcg
     2221 aggcgggcgc gatcggcgtc tcgaccgggc tcgagtacca g
//
LOCUS       DASTFC010000281         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1621802,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000281 DASTFC010000000
VERSION     DASTFC010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621802"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1182
                     /locus_tag="VFO88_08285"
     CDS             133..1182
                     /locus_tag="VFO88_08285"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9545562.1"
                     /translation="MSETVGILTGGGDCPGLNAVIRAVTRRSLDRGCRVVGVREGWRG
                     LVENRVEELGQREISGILPRGGTIVGTSRTNPYKSEDGVARVLATAKEIGLDALVAIG
                     GEDTLGVAARLHAEEGFPVVGVPKTIDNDLSGTDYTFGFDTAVFVATEAIDRLHTTAE
                     SHNRVMVVEVMGRHTGWIAVMSGIAGGADVILIPEQPMTVEECCDELRRRHGRGKDFS
                     IVVVSEGYELTYASGESRRVAGEPAATDQFGHVRLGGVGDELAREIEDRTGFETRVTV
                     LGHVQRGGTPTPHDRVLATRFGLKAADLVEERAWGQMAALQGDTIVAVPLEEAVKELK
                     RVPPEWYDVAKAFFG"
     gene            complement(1192..>2718)
                     /locus_tag="VFO88_08290"
     CDS             complement(1192..>2718)
                     /locus_tag="VFO88_08290"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9545563.1"
                     /translation="FGNSLYVWGALIGVVLTGLSVGYWLGGALADRVEPARLLFAVIG
                     LGGIGVLAVPLLDDRVLEWIVAWNPGPRLNPLIAAVVLFGPLSVVIAGVTPAAVRLRM
                     RSLARAGRTAGRLFSISTAGSIVGTLATAFFLIPELGIEQLFELAAATLFVAAGIVAL
                     LDRRLVAVVVAVAATGAAGYLAVDQEAGAGARVTGAATQNWSPFYRLRGYGYLDAHDA
                     QVAIEDESLTIVHAEDTAYHRLAVVEDEDTRYLRFDASLQSAMYIDDPLRTRFRYTDY
                     FHLGLAYNPGAEDVLFVGLGAGSSPKRMLADFPDLELHAVEIDPAVVDVAYRWFELPQ
                     DPRLTVETGDGRQLLAGSERRWDVIAIDAFFADAIPFHLVTQEFLELARSRLNPGGVI
                     VTNVIGALEGDGSRLFRSVYKTYRTVFPSVLVHPVIEEGQGGLDAFRNLIVVASESAP
                     ASRPVLADRWEEIRRETPSVPDLAAAIEDRRDQQVPTDDVPILTDDYAPTDALLFLFQ
                     "
BASE COUNT          420 a          909 c         1044 g          345 t
ORIGIN      
        1 cgctggactc aaaatccagt ggggctacgc cccgtgcggg ttcgactccc gccttcggca
       61 tccgactccc tcctgctcca tcctgtcccg tgacggagaa gacgtgaagc tgaccgcaaa
      121 gttagggagg cgttgagcga aacggtcggg atattgacgg gaggcggcga ctgccccggc
      181 ctgaacgcgg tcatccgcgc ggtgacccgc cgctcgctcg accgcggctg ccgggtcgtc
      241 ggggtgcgcg agggctggcg ggggctcgtg gagaaccgcg tcgaggagct cgggcagcgc
      301 gagatctccg ggatcctccc gcgcggcggg acgatcgtgg ggacgtcgcg gacgaatccg
      361 tacaagagcg aggacggcgt ggcgcgcgtc ctcgcgacgg cgaaggagat cgggctcgac
      421 gcgctcgtcg cgatcggcgg cgaggacacg ctcggcgtcg ccgcccgcct gcacgccgag
      481 gagggcttcc cggtcgtggg cgtcccgaag acgatcgaca acgacctctc cgggacggac
      541 tacacgttcg gcttcgacac cgccgtcttc gtcgccacgg aggcgatcga ccgcctgcac
      601 acgacggccg agtcgcacaa ccgcgtcatg gtcgtcgagg tgatgggccg ccacacgggc
      661 tggatcgccg tcatgagcgg gatcgcgggc ggcgccgacg tgatcctcat cccggagcag
      721 ccgatgaccg tcgaggagtg ctgcgacgag ctgcgccgcc ggcacggccg cgggaaggac
      781 ttctcgatcg tggtcgtgag cgaaggctac gagctcacgt acgcgtcggg cgagagccgc
      841 cgggtcgccg gcgagccggc cgcgaccgac cagttcggcc acgtccggct gggcggcgtc
      901 ggggacgagc tcgcacgcga gatcgaagat cggacgggct tcgagacgcg cgtcaccgtc
      961 ctcgggcacg tccagcgtgg cggaacgccc accccgcacg accgggtgct cgcgacccgg
     1021 ttcggcctga aggcggccga cctcgtcgag gagcgcgcct ggggccagat ggccgctctc
     1081 cagggcgaca cgatcgtcgc cgtcccgctc gaggaggccg tgaaggagct caagcgcgtc
     1141 ccgcccgagt ggtacgacgt ggcgaaggcc ttcttcggct gatccgctcg gctactggaa
     1201 gaggaagagg agcgcgtcgg tcggcgcgta gtcgtccgtg aggatcggga cgtcgtcggt
     1261 cgggacctgc tggtcgcggc ggtcctcgat cgcggcggcg aggtcgggga ctgagggcgt
     1321 ctcacggcgg atctcctccc accggtcggc gagcaccggg cggctcgcgg gcgcggactc
     1381 gctcgcgacc acgatgaggt tccggaacgc gtcgaggccg ccctggccct cctcgatcac
     1441 cgggtggacg agcacggacg ggaagacggt cctgtacgtc ttgtagaccg agcggaagag
     1501 gcgcgagccg tcgccctcga gcgcgccgat cacgttcgtg acgatcacgc cgcccgggtt
     1561 caggcgcgag cgcgcgagct cgaggaactc ctgcgtcacg aggtggaacg ggatcgcgtc
     1621 ggcgaagaac gcgtcgatcg cgatcacgtc ccagcgccgc tcgctcccgg cgaggagctg
     1681 gcgcccgtcc cccgtctcga cggtgagacg cgggtcctgc ggtagctcga accagcggta
     1741 ggcgacgtcg acgacggccg ggtcgatctc gaccgcgtgg agctcgaggt cggggaagtc
     1801 ggcgagcatc cgcttcggcg acgagccggc gccgaggccg acgaagagga cgtcctccgc
     1861 gcccgggttg tacgcgagcc cgaggtggaa gtagtccgtg tagcggaagc gcgtgcggag
     1921 cgggtcgtcg atgtacatcg cgctctggag cgacgcgtcg aagcggaggt accgggtgtc
     1981 ctcgtcctcg acgacggcga gccggtggta ggcggtgtcc tcggcgtgga cgatcgtgag
     2041 gctctcgtcc tcgatcgcca cctgcgcgtc gtgcgcgtcg aggtagccgt agccgcgcag
     2101 gcggtagaag ggcgaccagt tctgcgtcgc cgccccggtg acgcgcgcgc ccgcgccggc
     2161 ctcctggtcg accgcgagat agcccgccgc gccggtggcg gcgaccgcga cgacgaccgc
     2221 gacgagccgc cggtcgagga gcgcgacgat ccccgcggcg acgaagagcg tcgcggcggc
     2281 gagctcgaag agctgctcga tgccgagctc ggggatgagg aagaaggcgg tcgcgagcgt
     2341 tccgacgatc gagcccgcgg tcgagatcga gaagagccgc ccggccgtcc gccccgcgcg
     2401 tgcgagggag cgcatccgaa ggcgaacggc ggccggcgtc acgccggcga tcacgacgct
     2461 gagcgggccg aagagcacga ccgccgcgat cagcgggttc aggcgcgggc cggggttcca
     2521 ggcgacgatc cactcgagca cgcggtcgtc gaggagtggc acggcgagca cgccgatccc
     2581 gccgagcccg atcacggcga agagaaggcg cgccggctcg acgcggtcgg cgagcgcgcc
     2641 gccgagccag tagccgaccg agaggcccgt gaggacgacg ccgatgagcg cgccccagac
     2701 gtagagcgag ttcccgaa
//
LOCUS       DASTFC010000282         5689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_56255,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000282 DASTFC010000000
VERSION     DASTFC010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56255"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1800)
                     /locus_tag="VFO88_08295"
     CDS             complement(109..1800)
                     /locus_tag="VFO88_08295"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HET9545564.1"
                     /translation="MTRPADFPPPFSASEFVAEPIDPADERIDVGILIVGAGPGGLAA
                     AVRAGQLLQERPELAERLGEVPIAVLDKGRAVGAHLLSGAVVNPRAFRQLFPGMPSVD
                     FPFVGPVEREGVYYLTKGSAMRIPAPPPMWNHGNYVASVGDLSRWLAEKAEELGVMIV
                     PETAATKLLVSGGRVVGVRTGDRGRGKAGEELGNFEPGSDMKARVTILAEGTQGHLTG
                     VLLDRFGLQSRPQVWALGVKEVWSVPQKLERVIHTMGWPLRGAARHREFGGSFIYPLG
                     DDRVALGMVVGLDTTDATVSVHDLLQELKTHKKVRAILEGGERVAWGAKTIPEGGFLA
                     LPDRFHVPGAMIVGDGAGLVNVPALKGIHYAMRSGMLAAETAVDAVGPGRTAWTPDGL
                     AGYDQALRESYVWKDLERVRNMRPAFRHGFVRGAALAGAALNSLGKLPPRDLKLEGDG
                     AVPVRVGDRASAYPAPDGKLVFDKLSSVYLSGNKTRDDAPNHIRVQTAVAREVAEAWA
                     HMCPAQVYEVGAEAGDGLVHVEVTASNCVQCGAITAKGGRLTPPEGGSGPEYSSM"
     gene            complement(1813..2775)
                     /locus_tag="VFO88_08300"
     CDS             complement(1813..2775)
                     /locus_tag="VFO88_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET9545565.1"
                     /translation="MTTLVFVEHHEGEAAKGSLGVLAKAASLGGDVAAAVVGDNVRGV
                     AEGLGAHGASRVYVAEDASLAAPLPQPRVDVLARLVQDEGIDTVLFAASVLAADVAAA
                     LAARLGAGLNWDLVDLESRDGTLVGTTLAFGDTAKVEVGWKSEPRLGLVRSGAFEPAP
                     ADGGAPELRDVPVEVEEWSTQTAMLEQAHEESSGPSIEDADVIVAGGRGLGEAEKFAL
                     LEELAKALGGAVAATRAVVDAGWYPYATQVGQTGKNVSPKLYVACGISGAIQHKVGMQ
                     GSGTIVAINKDPNAPIFEYADLAVVGDLHEIVPKLTELVRQARG"
     gene            complement(2772..3554)
                     /locus_tag="VFO88_08305"
     CDS             complement(2772..3554)
                     /locus_tag="VFO88_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET9545566.1"
                     /translation="MKIAVCMKEVPDASAPKRLDPSTKRLDRSVEGALNDFDTHALEE
                     ALRVKDGAADTEVVVVSMGPAKAAESLRKALAMGADRLLLVSDDAAAGSDLLATASVL
                     AKALEREGPDLVLLGQQAGDSDGAVLWAALAERMRLPMISQAASLELDGGKARSKRQT
                     EYGYDVLEAPLPCVVAVSDAINEPRYPSLKGIMGAKKKPVDTVSLSDLGVDAGDVGEA
                     GSRTEVLGTGDPPPRGETQRIDDEGGDSAEKILAYLRERKLV"
     gene            3758..4549
                     /locus_tag="VFO88_08310"
     CDS             3758..4549
                     /locus_tag="VFO88_08310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9545567.1"
                     /translation="MVGPVTYTNDYGDPRGGGWHQGNDLMGKRRQPVIAAEPGRVTIY
                     RGSSRAGCMLYLYGASGTVYYYIHLNDDLTKYDDNRATDCRVGVAYAPGLKSGMRVRA
                     GHLIGFLGNSGDARGIQPHLHFEVHPNGGRPVNPYRHLGNADRLLFARPLGVDQVKLD
                     LMGKVVKTTEETFSVKVNRVGVATSEGRSSKRWLGRTITLAHTEETVWKRKLADGTMV
                     PASTAKAKPGERVTIWTPWFKPLLRTQRAKPGILTAERVRLRGEG"
     gene            complement(4546..5136)
                     /gene="thiE"
                     /locus_tag="VFO88_08315"
     CDS             complement(4546..5136)
                     /gene="thiE"
                     /locus_tag="VFO88_08315"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET9545568.1"
                     /translation="MFGDRRLYLIAPARPGLADLVEAAVRGGVDLVQIRDKELPDGEL
                     LRALEEAREVTRRLGVPLVVNDRPDLAVLVEADAVHVGQDDLPVAAVRRFRLPVGLST
                     HTPAEIDAAEANYIGVGPVHETPTKEGRPAAGLELVRHAAAHARQPWFAIGGIDRTNV
                     DEVLAAGAARIAVVRAIADAPDPETAARELRSALPV"
     gene            complement(5142..5348)
                     /gene="thiS"
                     /locus_tag="VFO88_08320"
     CDS             complement(5142..5348)
                     /gene="thiS"
                     /locus_tag="VFO88_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET9545569.1"
                     /translation="MSAAVTVNGREHELEAGETVERLLERLGLGARYALVERNGDPVE
                     RAAYGSVELAPGDTLVVARPVAGG"
     gene            complement(5345..>5689)
                     /locus_tag="VFO88_08325"
     CDS             complement(5345..>5689)
                     /locus_tag="VFO88_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET9545570.1"
                     /translation="EVGCATVMPLAAPIGTGRGLKLRDSIRIMIEEAEVPVVVDAGLG
                     APSHAAEAMELGADAVLVNTAIARADDPVTMARAFRLAVEAGRAGHLAGVMDEQATAQ
                     PSSPVGGLVTQP"
BASE COUNT          739 a         2123 c         2077 g          750 t
ORIGIN      
        1 gtgagcatcg gcgtgcggtg gagccggccc gcgacccggc gcgaggcgcg cgccacgtcg
       61 tccggcgtga cgaggagcga gccgtcggcc atgccgcgca gcctacgcct acatcgagga
      121 gtactcgggc ccggagccgc cttcgggcgg cgtgagccgg ccgcccttgg cggtgatcgc
      181 gccgcactgg acgcagttcg aggcggtgac ctcgacgtgg acgagcccgt cccccgcctc
      241 ggcgccgacc tcgtagacct gcgcggggca catgtgcgcc caggcctcgg cgacctcgcg
      301 cgcgaccgcc gtctgcacgc ggatgtggtt cggcgcgtcg tcgcgcgtct tgttcccgct
      361 caggtagacg ctcgagagct tgtcgaagac gagcttcccg tccggcgccg gataggccga
      421 ggcgcggtcg ccgacccgca cgggcaccgc gccgtcgccc tcgagcttga ggtcgcgggg
      481 cgggagcttt cccagcgagt tgagcgccgc cccggcgagc gcggcgccgc ggacgaaccc
      541 gtggcggaag gccggccgca tgtttcgcac gcgctcgagg tccttccaga cgtagctctc
      601 ccggagcgcc tggtcgtagc ccgctagccc gtccggcgtc caggccgtcc gccccgggcc
      661 gaccgcgtcg acggcggtct cggccgcgag catcccggag cgcatcgcgt agtggatccc
      721 cttgagcgcg ggcacgttca cgaggccggc cccgtcgccg acgatcatcg cgccgggcac
      781 gtggaagcgg tcggggagcg cgaggaagcc gccctccggg atcgtcttag cgccccaggc
      841 cacgcgctcg ccgccctcga ggatcgcgcg caccttcttg tgcgtcttga gctcctggag
      901 gaggtcgtgg acgctcacgg tcgcgtcggt cgtgtcgagg ccgacgacca tcccgagcgc
      961 gacgcggtcg tcgccgagcg ggtagatgaa gctcccgccg aactcgcggt ggcgcgcggc
     1021 gccgcgcagc ggccagccca tcgtgtggat gacgcgctcg agcttctgcg ggacgctcca
     1081 cacctccttg acgccgagcg cccagacctg cgggcgcgac tggagcccga agcggtcgag
     1141 gaggacgccg gtcaggtgcc cctgcgtgcc ttcggcgagg atcgtgacgc gcgccttcat
     1201 gtccgagccc ggctcgaagt tcccgagctc ctcgcccgcc ttgccgcggc cccggtcgcc
     1261 cgtccgcacg ccgacgacgc gcccgcccga gacgaggagc ttcgtcgcgg ccgtctccgg
     1321 gacgatcatg acgccgagct cctccgcctt ctccgcgagc cagcgggaca ggtcgccgac
     1381 ggacgcgacg tagttgccgt ggttccacat gggcggcggc gcggggatcc gcatcgccga
     1441 gcccttcgtg aggtagtaga cgccctcccg ctcgaccggc ccgacgaacg ggaagtcgac
     1501 ggacggcatc ccggggaaga gctggcggaa cgcgcgcggg ttcacgacgg cgcccgagag
     1561 gaggtgcgcg ccgacggccc tgcccttgtc gaggacggcg atcggcacct cgccgaggcg
     1621 ctcggcgagc tcggggcgct cctgcaggag ctggccggcg cgcacggccg ccgcgaggcc
     1681 gccgggcccg gcgccgacga tcaggatgcc gacgtcgatg cgctcgtcgg ccgggtcgat
     1741 cggctcggcg acgaactcgg acgcgctgaa cggcggcggg aagtcggcgg gcctcgtcac
     1801 ggcggcgtga gcttaaccgc gcgcctggcg gacgagctcc gtcagcttcg gcacgatctc
     1861 gtgcaggtcg cccacgaccg cgaggtcggc gtactcgaag atcggcgcgt tcggatcctt
     1921 gttgatcgcg acgatcgtgc cggagccctg cattccgacc ttgtgctgaa tcgcgccgga
     1981 gatcccgcac gccacgtaga gcttcggcga gacgttcttg cccgtctggc cgacctgcgt
     2041 cgcgtagggg taccagcccg cgtcgacgac cgcacgcgtg gccgcgaccg cgccgccgag
     2101 ggccttcgcg agctcctcga ggagcgcgaa cttctccgcc tcgccgagcc cgcgcccgcc
     2161 ggcgacgatc acgtccgcgt cctcgatcga ggggccggac gactcctcgt gcgcctgctc
     2221 gagcatcgcc gtctgcgtcg accactcctc cacctcgacg ggcacgtcgc gcagctcggg
     2281 cgcgccgccg tccgcggggg ccggttcgaa cgcgccggag cgcacgagcc cgaggcgcgg
     2341 ctcgctcttc cagccgacct cgaccttcgc cgtgtcgccg aacgcgagcg tcgtgccgac
     2401 gagcgtcccg tcgcggctct cgaggtcgac gaggtcccag ttgagcccgg cgccgagccg
     2461 cgccgcgagg gcggccgcga cgtcggccgc gaggacgctc gcggcgaaga gcaccgtgtc
     2521 gatgccctcg tcctgcacga gccgcgcgag cacgtcgacg cgcggctgtg ggagcggcgc
     2581 ggcgagcgag gcgtcctccg cgacgtagac gcgcgaggcg ccgtgcgcgc cgaggccttc
     2641 ggcgacgccg cggacgttgt cgcccacgac ggctgcggcc acgtcgccgc cgaggctcgc
     2701 cgccttggcg aggacgccga gcgagccctt cgcggcctcg ccctcgtggt gctcgacgaa
     2761 gacgagcgtc gtcatacgag cttccgctcc cgcaggtagg cgaggatctt ctccgccgag
     2821 tcgcccccct cgtcgtcgat ccgctgcgtc tcgccgcgcg ggggcgggtc gccggtgccg
     2881 agcacctccg tgcgcgagcc ggcctccccc acgtcgcccg cgtcgacccc gaggtcggag
     2941 agcgacacgg tgtcgacggg cttcttcttc gcgcccatga tccccttgag ggacgggtag
     3001 cgcggctcgt tgatcgcgtc cgagacggcc acgacgcacg ggagcggagc ctcgaggacg
     3061 tcgtagccgt actccgtctg gcgcttcgag cgggccttcc cgccgtcgag ctcgagcgag
     3121 gcggcctggg agatcatcgg cagccgcatc cgctcggcga gcgcggccca gagcacggcg
     3181 ccgtccgagt cgcccgcctg ctggccgagc aggacgaggt cggggccctc gcgctcgagc
     3241 gccttcgcga gcacggacgc ggtcgcgagg aggtcggagc cggccgcagc gtcgtccgag
     3301 acgaggagca gccggtcggc gcccatcgcg agcgccttgc gcagcgactc cgcggccttc
     3361 gccgggccca tcgagacgac cacgacctcg gtgtccgcgg ccccgtcctt cacgcggagc
     3421 gcctcctcga gcgcgtgcgt gtcgaagtcg ttcagcgcgc cctcgacgct ccggtcgagg
     3481 cgcttcgtgg acgggtcgag gcgcttcggc gcggaggcgt ccgggacctc cttcatgcac
     3541 acggcgatct tcacggccgc gagcatatcg ggcctcctcc gacggccttc gagttcacct
     3601 cctgctaagg tctgcgcctt cgcgcgccga cgcaccggag cagatgctca cacgccaccg
     3661 gacgacagcc cgaagcatcc ttctcgcctt tctcgtagcc gggagcctcg cgggaccttc
     3721 ggccgcgtcc gcagccgtcc cgaagctcgc gttcccggtg gtcggtccgg tgacgtacac
     3781 gaacgactac ggcgacccgc gcggaggcgg atggcatcag gggaacgacc tgatgggcaa
     3841 acgccgccag ccagtcatcg ccgccgagcc cggccgggtg acgatctacc ggggctcctc
     3901 gcgcgccggc tgcatgctct acctctacgg cgcgagcggg acggtctact actacatcca
     3961 cctgaacgac gacctgacga agtacgacga caaccgcgcg accgactgcc gcgtcggggt
     4021 cgcctacgcg ccgggcctca agagcggcat gcgcgtgcgg gcgggccacc tgatcgggtt
     4081 cctcgggaac tcgggcgacg cgcgcgggat ccagccgcac ctccacttcg aggtgcaccc
     4141 gaacggcggg cgcccggtca acccgtaccg gcatctgggg aacgccgacc ggctgctctt
     4201 cgcccgaccc ctcggcgtcg accaggtgaa gctcgacctc atgggcaagg tcgtgaagac
     4261 gacggaggag acgttctcgg tgaaggtcaa ccgggtcggc gtcgcgacct ccgagggtcg
     4321 gtcgagcaag aggtggctcg gccgcacgat cacgctcgcg cacaccgagg agaccgtctg
     4381 gaagcgcaag ctcgcggacg ggacgatggt cccggcgagc acggccaagg ccaagcccgg
     4441 cgagcgcgtg acgatctgga cgccgtggtt caagccgctt ctgaggacac agcgcgcgaa
     4501 gccggggatc ctcacggccg agcgcgtccg cctccgcggc gagggttaga cggggagcgc
     4561 cgagcggagc tcgcgggcgg ccgtctcggg gtccggagcg tctgcgatcg cgcggacgac
     4621 cgcgatgcgc gccgcgccgg cggcgaggac ctcgtcgacg ttcgtgcggt cgatgccgcc
     4681 gatcgcgaac cacggctggc gggcgtgcgc cgccgcgtgg cggacgagct cgaggccggc
     4741 agcgggccgt ccctccttcg tcggggtctc gtgcacgggg cccacaccga tgtagttcgc
     4801 ctcggcggcg tcgatttcgg cgggcgtgtg cgtcgacagg ccgacgggaa gccggaagcg
     4861 ccgcacggcc gcgacgggga ggtcgtcctg gcccacgtgg acggcgtccg cctcgacgag
     4921 caccgcgagg tcggggcggt cgttcacgac gagcgggacg ccgaggcgcc gcgtcacctc
     4981 acgggcctcc tcgagcgcgc ggaggagctc gccgtccggg agctccttgt cccggatctg
     5041 gacgaggtcg acgccgccgc gcacggcggc ctcgacgagg tcggcgagcc ccggccgcgc
     5101 cggcgcgatg aggtacagcc gtcgatcgcc gaacacggcg cctacccgcc ggcgaccggc
     5161 cgggcgacga cgagggtgtc gccgggggcg agctcgacgc tcccgtaggc ggcgcgctcg
     5221 accgggtcgc cgttccgctc cacgagcgcg tagcgggcgc cgaggcccag gcgctcgagc
     5281 agccgctcca ccgtctcgcc cgcctcgagc tcgtgctcgc gcccgttgac cgtcaccgcg
     5341 gcgctcacgg ctgcgtgacg agcccgccga cggggctcga cggctgcgcg gtcgcctgct
     5401 cgtccatcac gccggcgagg tgaccggcgc gcccggcctc gaccgcgagc ctgaacgcgc
     5461 gcgccatcgt caccgggtcg tccgcccgcg cgatcgccgt gttcacgagg acggcgtccg
     5521 ctccgagctc catcgcctcg gcggcgtgcg acggcgcgcc gagccccgcg tcgacgacga
     5581 cgggcacctc cgcctcctcg atcatgatcc ggatcgagtc ccgcagcttc agcccgcggc
     5641 cggtgccgat cggcgcggcg agcggcatga cggtggcgca gccgacctc
//
LOCUS       DASTFC010000283         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_831012,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000283 DASTFC010000000
VERSION     DASTFC010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831012"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /gene="mreC"
                     /locus_tag="VFO88_08330"
     CDS             <1..844
                     /gene="mreC"
                     /locus_tag="VFO88_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET9545571.1"
                     /translation="PLRARLVALGLVLVSLALLTVYFRESSGGPVHGAQRIVVSVLTP
                     FEAAAERVSRPFRDAYGWTSDLFHAKGEAADLRAEVKELRRQVILNQTAAQENEELRS
                     LLEYRDGPRFPNDYDAVATRVIVPAQTEFRQEVMVAAGSGDGVAVEDPVVTDAGLVGT
                     VTEVATNSAKVRLLTDQQSAASAFVPETGAAGMVERGSSTTSLVLDRVPKDEAVEEND
                     MVVTAGSRSRQLESLFPRGIPICVVTSVSQRDTDFYKRIQCTPLVDFDSLNQVIVLTR
                     KGRQ"
     gene            841..1359
                     /gene="mreD"
                     /locus_tag="VFO88_08335"
     CDS             841..1359
                     /gene="mreD"
                     /locus_tag="VFO88_08335"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET9545572.1"
                     /translation="MSWSDAAKALGLLVLLVLLQVTIVTPIEVASGHPDLVLVFLVSL
                     ALLRGPMLGAVAGFWAGLLLDSAALAPPGVSSLVLTLAGYWAGRFGVATTRSSPHPPI
                     IAVALATVWVAVGLTVVNFLLGQSVPAGELFGRVLLPTLALNVLITYPVYRLTERLFP
                     VAPRAGREAVLA"
     gene            1352..3319
                     /gene="mrdA"
                     /locus_tag="VFO88_08340"
     CDS             1352..3319
                     /gene="mrdA"
                     /locus_tag="VFO88_08340"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9545573.1"
                     /translation="MRNAPGFLPPDPRVEEPYRLTPQLAVRIAIIGVVAIALFGALFF
                     RLWALQVISGAEYLADAQNNQVRTFRVQANRGPILDRDGDVLVSNRPGTRVQLWPAAL
                     DELPQAQRSEMIKELGKLLDLGPQEIRETLKKHRDDPLTPVTIKDGVKREKVVYLLEH
                     QARFPGVQVTQIELRRYERGTMAAQVLGYVSEISQEQLDAREGSDYAPGDRIGQGGVE
                     AAYDRYLRGVAGVGQVRVDAMGRVTSNREFSQMPEAGFAVKLTLDADLQAAAEDALDY
                     GIRLAHENGDWAADGGAIVAMDPNTGELLAVASSPTFDPSVYVGRVRPKSLERLAAPE
                     ANSPTLNRAIAGLYPPASAFKPVIALAAIHEGFLSPAELIQCSPQIEVGEDKQVFRNW
                     DPYRNEPMELTTALAASCDTYFYEVALRAYERPDTPFQKWATRMGFGSTTGVDIGPES
                     EGLVPTPAWRRRHFEHPWDKEWRPGDSVQLSIGQGDMLVTPLQMARFYSLIANGGLLV
                     EPRIVKQIEQPSTESGAPVVIRPFTAPKPKDIGLDPNAVAIVQEGLYDATHSSYGTST
                     AVFGEFPISIAGKTGTAEKFVTLPGFQGLMDQSWFCGYGPREQAEIVVCALIENGGHG
                     GEAAAPAALKVFESYFNVQPGSYAAQAVESD"
     gene            3324..>3599
                     /locus_tag="VFO88_08345"
     CDS             3324..>3599
                     /locus_tag="VFO88_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545574.1"
                     /translation="MAVRVADTRRRPEVSIGGEAGRILRNLDYVLLGAVAALVAYGLW
                     VLASVSQNDVPGDPDFFLVRQSVNVAIGVAVLALATALNPELYRRLRV"
BASE COUNT          486 a         1342 c         1245 g          526 t
ORIGIN      
        1 ccctctcagg gcccggctcg tcgccctcgg gctcgtcctc gtctcgctgg cgctcctgac
       61 ggtctacttc cgagagtcct cggggggtcc cgtccacggc gcccagcgga tcgtcgtcag
      121 cgtcctgacc ccgttcgagg cggccgccga gcgggtgagc cgcccgttcc gcgacgcgta
      181 cggctggacg tccgacctct tccacgcgaa gggcgaggcc gccgacctgc gcgcggaggt
      241 gaaggagctc cggcggcagg tgatcctgaa ccagaccgcc gcgcaggaga acgaggagct
      301 ccgcagcctg ctcgagtacc gcgacgggcc tcgcttcccg aacgactacg acgcggtcgc
      361 gacgcgcgtg atcgtccccg cgcagaccga gttccgccag gaggtcatgg tcgcggccgg
      421 ctcgggcgac ggggtcgccg tcgaggaccc ggtcgtcacc gacgcgggcc tcgtcggcac
      481 cgtgaccgag gtcgcgacga actcggcgaa ggtgcgcctg ctcaccgacc agcagagcgc
      541 cgcgtccgcg ttcgtccccg agaccggcgc cgccgggatg gtcgagcgcg gctcgagcac
      601 cacgtcgctc gtgctcgacc gggtgccgaa ggacgaggcg gtcgaggaga acgacatggt
      661 cgtgacggcc ggctcccgct cgcgccagct cgagtccctc ttcccccgcg ggatccccat
      721 ctgcgtcgtc acgagcgtca gccagcggga caccgacttc tacaagcgca tccagtgcac
      781 gccgctcgtc gacttcgact cgctgaacca ggtgatcgtc ctcacgcgga agggccgtca
      841 gtgagctgga gcgacgccgc gaaggcgctc ggcctgctcg tcctgctcgt gctcctccag
      901 gtcacgatcg tgaccccgat cgaggtagcg agcggccatc cggacctcgt cctcgtcttc
      961 ctcgtctcgc tcgcgctcct gcgtgggccg atgctcgggg cggtcgccgg cttctgggcg
     1021 gggctcctgc tcgactcggc cgcgctcgcg ccgccgggcg tctcctcgct cgtcctcacg
     1081 ctcgccggct actgggccgg ccgcttcggg gtcgcgacga cgcgctcctc gccccacccg
     1141 ccgatcatcg ccgtcgcgct cgccaccgtc tgggtcgcgg tcgggctcac ggtcgtgaac
     1201 ttcctcctcg ggcagagcgt cccggccggc gagctattcg ggcgcgtgct cctgccgacg
     1261 ctcgcgctga acgtcctcat cacgtacccg gtctaccggc tgaccgagcg gctgttcccg
     1321 gtcgctccgc gcgccgggcg ggaggccgtc cttgcgtaac gcgcccgggt tcctcccgcc
     1381 cgacccgcgg gtcgaagagc cctaccggct gacgccgcag ctcgccgtgc ggatcgcgat
     1441 catcggcgtc gtcgcgatcg cgctcttcgg cgcgctcttc ttccgcctct gggcgctcca
     1501 ggtgatctcg ggcgccgagt acctcgcgga cgcccagaac aaccaggtgc ggacgttccg
     1561 ggtgcaggcg aaccgcggcc cgatcctcga ccgtgacggc gacgtgctcg tctcgaaccg
     1621 gccggggacg cgcgtccagc tctggccggc ggcgctcgac gagctgcccc aggcgcagcg
     1681 ctcggagatg atcaaggagc tcgggaagct gctcgacctc gggccgcagg agatccgcga
     1741 gacgctcaag aagcaccggg acgacccgct cacgccggtc acgatcaagg acggggtgaa
     1801 gcgggagaag gtcgtctacc tcctcgagca ccaggcgcgc ttccccggcg tgcaggtgac
     1861 gcagatcgag ctgcgccgct acgagcgggg gacgatggcc gcgcaggtcc tcggctacgt
     1921 gtcggagatc tcccaggagc agctcgacgc gcgcgagggc tcggactacg cgccgggcga
     1981 ccggatcggc cagggcgggg tcgaggcggc gtacgaccgc tacctccgcg gcgtcgcggg
     2041 tgtggggcag gtgcgcgtcg acgcgatggg ccgcgtcacg agcaaccgtg agttcagcca
     2101 gatgccggag gcgggcttcg ccgtcaagct caccctcgac gccgacctcc aggcggccgc
     2161 ggaggacgcg ctcgactacg ggatcaggct cgcccacgag aacggcgact gggccgcgga
     2221 cggcggcgcg atcgtcgcga tggacccgaa cacgggcgag ctcctcgcgg tcgcgtcttc
     2281 gccgacgttc gacccgtccg tctacgtcgg ccgcgtgcgg cccaagtcgc tcgagcgcct
     2341 cgccgccccg gaggccaact cgccgacgct caaccgcgcg atcgccgggc tctatccgcc
     2401 ggcgtccgcg ttcaagccgg tcatcgccct cgccgcgatc cacgaggggt tcctgagccc
     2461 cgccgagctc atccagtgct cgccgcagat cgaggtcggc gaggacaagc aggtcttccg
     2521 gaactgggac ccgtaccgga acgagccgat ggagctcacg accgcgctcg ccgcctcgtg
     2581 cgacacgtac ttctacgagg tcgccctccg tgcgtacgag cgcccggaca cgccgttcca
     2641 gaagtgggcg acgcgcatgg gcttcggctc gacgaccggc gtcgacatcg gcccggagtc
     2701 cgagggcctc gtcccgaccc ccgcctggcg gcgccgccat ttcgagcacc cttgggacaa
     2761 ggagtggcgg ccgggcgact ccgtccagct ctcgatcggg cagggcgaca tgctcgtgac
     2821 cccgctccag atggcgcgct tctactcgct gatcgcgaac ggcggcctgc tcgtcgagcc
     2881 gcggatcgtg aagcagatcg agcagccgtc caccgagagc ggcgccccgg tcgtcatccg
     2941 cccgttcacg gcgccgaagc cgaaggacat cgggctcgac ccgaacgccg tcgcgatcgt
     3001 ccaggagggc ctctacgacg cgacccactc ctcgtacggg acgtcgaccg cggtcttcgg
     3061 tgagttcccc atctcgatcg ccggcaagac gggcacggcc gagaagttcg tgacgctccc
     3121 gggcttccag gggctcatgg accagtcctg gttctgcggg tacgggccgc gcgagcaggc
     3181 cgagatcgtc gtctgcgcgc tcatcgagaa cggcggccac ggcggcgagg cggccgcgcc
     3241 cgccgcgctc aaggtcttcg agagctactt caacgtccag ccggggtcgt acgccgccca
     3301 ggcggtggag agcgactagc gcgatggccg tccgtgtagc cgacacccgt cgccggcccg
     3361 aggtctccat cggcggagag gccgggcgga tcctccgcaa cctcgactac gtcctgctcg
     3421 gcgcggtcgc ggccctggtc gcctacgggc tctgggtcct cgcctcggtc tcgcagaacg
     3481 acgtgccggg cgacccggac ttcttcctcg tgcgccagtc ggtgaacgtc gcgatcggcg
     3541 tggccgtgct cgcgctcgcg acggcgctca acccggagct ctaccgccgc ctgcgcgtc
//
LOCUS       DASTFC010000284         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1539661,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000284 DASTFC010000000
VERSION     DASTFC010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539661"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFO88_08350"
     CDS             complement(<1..364)
                     /locus_tag="VFO88_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545575.1"
                     /translation="MALPLESVPNVSEGRDPRALEALREAFSAPARLLDVHADPDHHR
                     SVYTLVGTDDALVETLLAGARRAVEVVDLRRHEGVHPRIGALDVCPIVPLRPEDEERA
                     RVAALALADRLAGELGLPV"
     gene            429..977
                     /locus_tag="VFO88_08355"
     CDS             429..977
                     /locus_tag="VFO88_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545576.1"
                     /translation="MSSEPPHVRRPRRLWVVLSVLVVLAGVLVAAAFAVLRGQPPDFA
                     PTGACCTPANGEPPYIGGPLANDGRFTVRITAVEGPPGTETLLVRAGVPGTIEAAEPF
                     APFDLEPGTFRDVFLLAEAPGCTAAAVEEAGSRSGSSTVRIAYRQFGIDRSTVVDADE
                     MGTLFVAETPDGSCAYLDPPGR"
     gene            complement(974..1384)
                     /locus_tag="VFO88_08360"
     CDS             complement(974..1384)
                     /locus_tag="VFO88_08360"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET9545577.1"
                     /translation="MSETAARRATAALALVGAAVAAYLTITWAADVAPVCTTGGCSEV
                     QSSEYAELLGIPVAALGLGAYLAILATALRGGPGPAAIGAALAFAGLAFSLYLLVIQA
                     FVIEAFCLWCLVSDAVMLLLAAVAVFRLRAASPA"
     gene            complement(1381..2187)
                     /locus_tag="VFO88_08365"
     CDS             complement(1381..2187)
                     /locus_tag="VFO88_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9545578.1"
                     /translation="MARRSPTRARGGKVAPTPKPPPVGQKKKGGTGFLSWTWAAVGLA
                     VVLAAGLILVSVLTGDDEPTTSEITPAGTVAGAGEVEQLLSGIPQDGVALGDPEAPVT
                     LLEFADMQCPFCREWAWDAFPTLVEEYVREGQLRIEFRPMAFIGEDSGRGARAALAAG
                     EEDRLWHVVELLFRAQGAENSGWVSDELLGAIGSSVDGLDGDMLVSAAEGDEGLDEGI
                     AESTAEANANGINSTPSFMLGRTGGELQKIEVSSLGPEGLREQIDALLRR"
     gene            2338..4143
                     /locus_tag="VFO88_08370"
     CDS             2338..4143
                     /locus_tag="VFO88_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9545579.1"
                     /translation="MNLVLANTDPQPSPPFRRTFVRLLGFLRPYKWSLVASVVLAIGS
                     QAAGLALPALTGYAIDHALPDQDERLLQLIVGLIALAGLVKSLMLVGRRFIAGRQALG
                     VEFDLRNALYSRLLRLSWSFYDRHQTGQLMSRATVDLQGVRFFLGYGLIFFGQHILTV
                     VAASAILFFYNWQLALIPFAITPLLVAIAYRYSRASHPILKDVQQRMADMTTVAEENI
                     VGVNVVKAFAQEEREERKFAARSEEVFTRSLDATRQRAFYVPVLSFLPLLAQAAVLLV
                     GGRLVVNGELELGAFIAFNIYVLMLIMPLRMLGMWIGTAQRAVASGERIFEILDEPEE
                     IADRPDARPLPPGDGRLVFDGVTFGYDPERPVLHGIDIAIEPGSTVALIGHTGSGKTT
                     LTALVPRFYDVQEGAVLLDGADVRDVRLEDLRRAIAIVSQDPFLFSTTVRENIAFGVP
                     GATDEEVERAARMAQAHDFVSALPEGYDTVIGERGITLSGGQRQRLAIARAILVDPRV
                     LVLDDATASVDATTEAQIRLGLREAMRGRTTLIVAHRLSTISLADEIVVLDHGRVAAR
                     GPAEELAETSPVYREIVEHGLVEARVMTEREEATA"
BASE COUNT          545 a         1562 c         1500 g          579 t
ORIGIN      
        1 agaccgggag cccgagctcc ccggcgagcc ggtcggcgag cgcgagcgcg gcgacccggg
       61 cgcgctcctc gtcctccggt cggagcggga cgatcgggca gacgtcgagc gcgccgatgc
      121 gcgggtggac gccctcgtgc ctgcgcaggt cgacgacctc gaccgcccgg cgggcgcccg
      181 cgagaagcgt ctcgacgagc gcgtcgtccg tcccgacgag cgtgtagacg gagcggtggt
      241 ggtccgggtc ggcgtggacg tcgaggagcc gcgcgggcgc ggagaacgcc tcgcggagcg
      301 cctcgagcgc gcggggatcg cggccctccg agacgttggg gacagactcg agcgggagcg
      361 ccaccgcgcg aggatacgtc ccttttggac gcgttgacag ccccggcgcc gcgccgcagg
      421 cttgacccgt gtcgagcgag ccgccgcacg tccgccgtcc gcgccgcctc tgggtcgtcc
      481 tctcggtgct cgtcgtgctc gcgggcgtcc tcgtcgcggc ggcgttcgcc gtcctccgcg
      541 gccagccgcc ggacttcgct ccgaccggcg cctgctgcac gccggcgaac ggggagccgc
      601 cgtacatcgg cgggccgctc gcgaacgacg gacggttcac ggtgcggatc accgcggtgg
      661 aaggaccgcc cgggaccgag accctgctcg ttcgcgcagg cgttccgggg acgatcgagg
      721 ccgccgagcc gttcgcgccg ttcgacctcg agcccggcac gttccgcgac gtctttctgc
      781 tcgcggaggc gccgggctgc acggccgccg ccgtcgagga ggcgggctcg cggtcggggt
      841 cgagcacggt gcgaatcgcg taccggcagt tcggcatcga ccgctccacg gtcgtcgacg
      901 cggacgagat gggcacgctc ttcgtcgccg agacgcccga cggctcctgc gcgtacctcg
      961 atcctccggg gcgctaggcc ggcgaggcgg cgcgcagccg gaagaccgcg accgccgcga
     1021 gcaggagcat caccgcgtcg ctcacgaggc accagaggca gaacgcctcg atcacgaacg
     1081 cctggatcac gagcaggtac aggctgaacg cgagccccgc gaacgcgagc gccgcgccga
     1141 tcgccgccgg cccggggccg ccgcgaagcg cggtcgcgag gatcgcgagg tacgcgccga
     1201 gcccgagcgc cgcgaccggg atcccgagga gctccgcgta ctcggagctc tgcacctcgg
     1261 agcacccgcc cgtcgtgcag acgggcgcga cgtcggccgc ccaggtgatc gtgaggtagg
     1321 cggcgaccgc cgcgccgacg agcgcgagcg cggcggtcgc acgacgggcg gcggtctcgc
     1381 tcaccggcgc aggagcgcgt cgatctgctc ccgcagcccc tcgggcccga gcgagctcac
     1441 ctcgatcttc tggagctcgc cgcccgtccg gccgagcatg aacgacggcg tcgagttgat
     1501 cccgttcgcg ttcgcctccg ccgtcgattc ggcgatcccc tcgtcgagcc cctcgtcgcc
     1561 ctccgccgcg gagacgagca tgtcgccgtc gagcccgtcg accgaggagc cgatcgcccc
     1621 gagcagctcg tcggagaccc agccggagtt ctccgcgccc tgcgcgcgga agaggagctc
     1681 gacgacgtgc cagaggcggt cctcctcgcc cgccgcgagc gccgcgcggg caccgcggcc
     1741 ggagtcctcg ccgatgaacg ccatcgggcg gaactcgatc cggagctgtc cttcgcgcac
     1801 gtactcctcg acgagcgtgg ggaacgcgtc ccaggcccac tcgcggcaga acgggcactg
     1861 catgtcggcg aactcgagca gcgtcacggg cgcctccggg tcgccgagcg cgaccccgtc
     1921 ctgcgggatc ccgctgagga gctgctcgac ctcgcccgcg cccgccaccg tgccggccgg
     1981 cgtgatctcg gaggtcgtcg gctcgtcgtc gccggtgagc acgctgacga ggatgaggcc
     2041 ggcggcgagc acgacggcga ggccgactgc ggcccacgtc caggagagga agcccgtccc
     2101 gcccttcttc ttctggccga cgggaggcgg cttcggggtc ggggcgacct tgccgccgcg
     2161 cgcgcgggta gggctccgtc tcgccatggc gtcgtacctc ctgggctcgt cctggactgg
     2221 ggaccttagt gtcggcactc ggagcgccct cgcgcatccg gaccggccac ggtcgcgctt
     2281 gcgggaaact cccgagcggg aaggccgggc gacggaacac cgccgctaga ctcgtcgttg
     2341 aacttagtct tggctaacac cgatccgcag cccagcccgc cgttccggcg caccttcgtg
     2401 cgcctgctcg ggttcctgcg cccgtacaag tggtcgctcg tcgcgtcggt cgtgctcgcg
     2461 atcggctcgc aggcggccgg tctcgcgctc cccgcgctga ccgggtacgc gatcgaccac
     2521 gcgctccccg accaggacga gcggctgctc cagctcatcg tcgggctgat cgcgctcgcc
     2581 gggctcgtga agtcgctcat gctcgtcggg cggcgcttca tcgccggccg ccaggcgctc
     2641 ggcgtcgagt tcgacctccg aaacgcgctc tactcgcggc tcctccgcct ctcgtggagc
     2701 ttctacgacc gccaccagac gggccagctc atgtcccgcg cgaccgtcga tctccagggc
     2761 gtgcggttct tcctcggcta cgggctcatc ttcttcggcc agcacatcct cacggtcgtc
     2821 gcggcgagcg cgatcctctt cttctacaac tggcagctcg cgctgatccc gttcgcgatc
     2881 acgcccctcc tcgtggcgat cgcgtaccgc tactcgcgcg cgtcgcaccc gatcctgaag
     2941 gacgtccagc agcggatggc ggacatgacg accgtcgccg aggagaacat cgtgggcgtc
     3001 aacgtcgtga aggcgttcgc gcaggaggag cgcgaggagc ggaagttcgc ggcgcgcagc
     3061 gaggaggtct tcacgcgctc gctcgacgcg acgcgccagc gggcgttcta cgtgccggtg
     3121 ctgtcgttcc tgccgctcct cgcccaggcg gcggtgctgc tcgtcggcgg gcggctcgtc
     3181 gtgaacggcg agctcgagct cggcgcgttc atcgcgttca acatctacgt cctcatgctg
     3241 atcatgccgc tgcgcatgct cggcatgtgg atcgggacgg cgcagcgcgc ggtcgcgtcc
     3301 ggcgagcgca tcttcgagat cctcgacgag ccggaggaga tcgccgaccg gcccgacgcg
     3361 cggccgctcc cgcccggcga cggacggctc gtcttcgacg gcgtgacgtt cggctacgac
     3421 cccgagcggc ccgtgctgca cgggatcgac atcgcgatcg agccgggctc gacggtcgcg
     3481 ctcatcgggc acacgggttc cgggaagacg acgctgaccg cgctcgtccc gcgcttctac
     3541 gacgtgcagg agggcgcggt gctcctcgac ggcgccgacg tgcgcgacgt tcggctcgag
     3601 gacctgcgcc gcgcgatcgc gatcgtctcg caggatccgt tcctcttctc gacgaccgtc
     3661 cgcgagaaca tcgccttcgg cgtgccgggc gcgaccgacg aggaggtcga gcgcgccgcc
     3721 cggatggcgc aggcccacga cttcgtctcc gcgctcccgg agggctacga caccgtgatc
     3781 ggcgagcgcg gcatcacgct ctccggcggg cagcgccagc ggctcgcgat cgcccgcgcg
     3841 atcctcgtcg acccgcgcgt gctcgtcctc gacgacgcga ccgcctcggt ggacgcgacg
     3901 accgaggcgc agatccggct cggcctgcgc gaggcgatgc gcggccggac gacgctcatc
     3961 gtcgcgcacc ggctctcgac catctccctc gcggacgaga tcgtcgtcct cgaccacggg
     4021 cgcgtcgccg cccgcgggcc ggccgaggag ctcgcggaga cgagcccggt gtaccgcgag
     4081 atcgtcgagc acggcctcgt cgaggcccgc gtgatgaccg agcgcgagga ggcgacggca
     4141 tgaggatgca ccagccgggc tcgcacctca cgcaggcgcg cggcgc
//
LOCUS       DASTFC010000285        14686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1572560,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000285 DASTFC010000000
VERSION     DASTFC010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572560"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1159)
                     /locus_tag="VFO88_08375"
     CDS             complement(95..1159)
                     /locus_tag="VFO88_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545580.1"
                     /translation="MADSETHDDVARAQIEALFDRGEEAGCVNLGEATALVQELGLDD
                     DATEALFDELDSRGIEVTDDCGREGASPTYVNGDLASSTTDALQLFLNEVGRHKLLTA
                     DEEVALAKRIERGDEQAKEQMVNANLRLVVSIAKRYQGHGLSLLDLIQEGIIGLIRAV
                     EKFDWRKGFKFSTYATWWIRQAVQRGVANKSRTIRIPVHIVEREQRIARAERELVVKL
                     GRDPTDQEIAKAARLSVKQVREVRAAARAVTSLDKPVGDDDGTALGELFAGESDMPEE
                     EIDVSLREEALRQALDDLPDRARDILRLRYGLGGQDPQSLESIGRDLGLTRERVRQIE
                     QEALSRLAVSRELEALREAA"
     gene            1303..4209
                     /locus_tag="VFO88_08380"
     CDS             1303..4209
                     /locus_tag="VFO88_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545581.1"
                     /translation="MEPTPRRLTRTLFRIAFAAALALAVLTAGPAGAGPAGPTLIGPT
                     DGAAVEMLPVFAWQAVTNADRYELELSADPGFNSAIATVSTRNTRATLKTAIANGTYY
                     WRVRAVTAAGALGSWSASRIVEMAWTANPSLLSPGDGAILDYPSDALRLSWTPVEGAS
                     EYLVRIATDPALGSLIWASGPVKTAATGYTLTAPIAPGTYYWAITPLNAQGHAGTPSS
                     TQSFQWRWPSTTTPIFTDLGPQPEIIDPFFSWPRVPGAAGYEVEVNSSSDWAPGSRVC
                     CDAVRFGTGISTLGLSLAPLVQLDNNTYYWRVRPIDPSGNAGSWSVGPSFTQTFANTP
                     PTPAPSVKNLRVRDNVGDPYDGLDPASVPYPISTAAPVLTWNPVPGASSYQVDVTPYE
                     GGACDWSYPTTGHWVKTTTTNAWTPLGWSWNNVKPFPNAASVSNETTSLVAGRTYCAR
                     VRPQDGASAAGGPLVSGDWTYLPANNSPAFTWTGSPAPGECSCELAASDYNTPVTGST
                     LARMPLFTWDAVPGAQSYFVLVSRDPNFTNLVDYAFTKIAAYAPRTSNQTKGYPDELT
                     SYYWAVLPARDLDGSGVSANPLDGTPPSFEKQAVPPTLVAPDPGAVLDGPTTFRWTNA
                     EAARRYRIQVSQDPSFANPIDDTVTDSTAYTSNTTYPANTTLYWRVRADAENGTGYVG
                     LRWSDTGTFTKQLPAPVPDPGNPTGGALIPTWSWAPVPGAVSYDVTVEEPDGDRRNFD
                     DLPLHAFTPVKMTGVGIFTAQVRANFPTATSQVVPGPYSPLMPFARTIPEPGGAAADK
                     GANHLLFVWNPRPGAKQYRLQVSTRADFALPVETVLTQAPNHAPVLTQRDYMDGGTFF
                     WRVAAIDADGNIGDYSPTQQVATTQRIRLSAYGSPYVRRKTTIIVSTRTSRGPIAGVN
                     VRLWGAGLRARTKRTAADGRVTFGITPRKRGRIYIRATKAGYFTTQTSIPVRRR"
     gene            4468..5730
                     /gene="lysA"
                     /locus_tag="VFO88_08385"
     CDS             4468..5730
                     /gene="lysA"
                     /locus_tag="VFO88_08385"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET9545582.1"
                     /translation="MLALFPDTADAAGGELSLGGVPVSRLAAGHGTPLVVLCAETLRA
                     RARAYLDAAPDALVAYSVKAFPNVAVLRELAELGLGADVSTLGELSFALAAGVPGERI
                     VVHGNNKSDEELRAAAEARVRHVVLDSEDEVERAAAAGVTRVLVRVTPGIDADTHAAI
                     RTAHAGSKFGLPPEAALRAIEAARSAGLDVAGIHVHIGSQLLDTQTARSSVDWAATFA
                     ADCRAELGWTPSVLNVGGGLGIAHVEDERPPSIGDFVGALAGRIRHEWELLGLPLPSL
                     VLEPGRSIVGPAGVTLYRVGVVKRASESTTWVAVDGGMSDNPRPALYGARLTAVLAER
                     LDEEPAGPYTVCGKHCESGDVLVDRVALPEPRRGDLLAVPATGAYTLAMASTYNAVPR
                     PAAVLVEKGEARVIRRRETVAELLAHET"
     gene            complement(5741..6988)
                     /gene="hemL"
                     /locus_tag="VFO88_08390"
     CDS             complement(5741..6988)
                     /gene="hemL"
                     /locus_tag="VFO88_08390"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET9545583.1"
                     /translation="MSASGELYRRALARIPGGVNSPVRAMKSVGLEAPLFAASGEGAY
                     LVDADGRRYVDWVMSWGPLLLGHADPETVAAVTEAARRGTSFGASTEAEVELAEEIAD
                     AVPSIESVRLVSSGTEAAMSAIRLARAATGRDRILKFAGCYHGHADALLAEAGSGLAT
                     LGIPSTPGVPSAVTADTLVAPYNDLAAVEEAADESLAAVLVEPVAGNMGVVPPEPGFL
                     EGLRAVCDRVGALLVLDEVITGFRVARGGAQERFGVRPDLTILGKILGGGLPVGAFGG
                     RRDVMERLAPAGDVYQAGTLSGNPLATAAGLSVLRRLRDPAVYEELERRGARLEAGLR
                     ETGATVQRIGAMLTVFQREGAVRNLAEAQASDTDRYAAVFRALLERGVYVAPSQYEAM
                     FVSLAHGDEEIDRTVEAFAAASK"
     gene            complement(6985..8274)
                     /gene="hemA"
                     /locus_tag="VFO88_08395"
     CDS             complement(6985..8274)
                     /gene="hemA"
                     /locus_tag="VFO88_08395"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9545584.1"
                     /translation="MGSNGRHSPALVQVGVSHHSAALDVRELLHVPPNELAAVAQQLA
                     DEGFEAAVLSTCNRTELYLAGHDAALAAAVARGALARRSGLAEADLESLLEERRDAEA
                     AHHLFRVTAGLESLLPGEAQIIGQVREAYDVASGAGTTGPVLNRLFEHALHCGKQVRT
                     DTGVGTLPAAIPAAAAALAERTIGKLDGKRILVIGAGKMGELAATSFLARGAERVFVA
                     NHRLERAEQLAARFGGEAVPFEQIGEQLARADVVLSSTRCPETILHADEVEQALAGRE
                     DHPLLLVDIAVPRDLDPAIADLDGAILYDLDALGPEAGDALAHRDEQWEAAERIAAGG
                     AGELAAWLRSLEVVPVVTALRRRADEIREAELERVAPRLRTLSDEERREVEALTAQIV
                     NKLLHEPTVRVKEAASEGAEEYAAALRKLFALDEERA"
     gene            8516..9136
                     /locus_tag="VFO88_08400"
     CDS             8516..9136
                     /locus_tag="VFO88_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HET9545585.1"
                     /translation="MAYDVTTRAGGWVAAQAARRWRRASLVGGVGLLATIVLVTSTLA
                     GRTHPLLVLGVLAVALVAYRIADSMLDDAVHWRDGARAEGAVGDLLDTLRPEFVVMHD
                     LRQLGEGNVDHLVSGPSGVFMIETKARRYPPNALRKAKRQAAKLGNELEVWVTPVICI
                     HERRGSEPFNHHGVWIVSSERLPAWLRTQRNATLPFERLARFADSL"
     gene            9167..11329
                     /locus_tag="VFO88_08405"
     CDS             9167..11329
                     /locus_tag="VFO88_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9545586.1"
                     /translation="MTERLARASALHPVRTLLAWVGGIVLAIVLVAMLLGGNLTSEGN
                     VTNNPESVRANAILAESLPVEDTASELVVVRSNDVLMVDTAVRLAVEEMLAEAGDAVE
                     QAELWHESQNPTLLSEDRAALLVPIELRAPEEETVEELVAAVQAADERSENLNFEVTG
                     RYTTGADFTEVSERDLQEGELRFGLPSALVVLVLVFGALVAALMPLALALVSIIVALG
                     LTALVAQQWDLSVFVVNMLTGMGLALGIDYALFVVSRFREERAHGREKLDAIVKTGST
                     ASRAVLFSGTAFVFAMTGLLLVQSTIMRSLAVGAILVGIVSVAAALTLLPALLSLLGD
                     RVNRLRVPFVAREIERDSAQESRFWGWIVQRVIRRPALSFAVAAAALLALAVPLLGMN
                     IGAAGIGTLPDALVSKQGLVALQDSFPRAGAEPARIVVQGDVASPEIEGAFERLEAEL
                     AEDGTFGPPVLEQAPDGDVAVMTVPVAADALSDQAIDAVERLRGDYVPAAFEGVEAEP
                     LVGGTTANNIDYFDVMKRWLPLVFVFVLGLSFILLTVAFRSIVIAGTAIVLNLLSIGA
                     TYGLLVLVFQHGWGTDLLGFQQVDIIEAWVPLFLFAVLFGLSMDYQVFLLSRIRERFT
                     QTGSTNDAVAFGIGSTARLITGAALIIVAVFVGFAMGDLVMFQQMGFGIAVALLIDAT
                     VVRSVLVPAAMTLLGSWNWYLPGWLQWLPHMEVEGHTD"
     gene            complement(11326..11742)
                     /locus_tag="VFO88_08410"
     CDS             complement(11326..11742)
                     /locus_tag="VFO88_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545587.1"
                     /translation="MRLEPLYRATFSTPEHWSVELAGEHGTEGQTFLIAEGRLDGRLS
                     ARLRGANFPRRRVDGTLTPDFRGVYETDDGATILFSWHGYGRRGRLAGAITHLSDDER
                     YRWLNDTVCALTGEVREREDGPGVDVVLEVAEIVDR"
     gene            complement(11742..12749)
                     /locus_tag="VFO88_08415"
     CDS             complement(11742..12749)
                     /locus_tag="VFO88_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545588.1"
                     /translation="MNVYVQTNEGRENGVLAFRRGDDGALAPLGAYATGGAGDGVPHL
                     TSQGSVVLSGDGSRLFVTNAGSGSVSVFAVAEGGLELRAAVPTGAAPKSVAEHGGLVY
                     VLNTGMPSLAGFRVTDGGLEPLAGSERALAAGSDPAQVGFAPDGSKLVVTDRGTDSIA
                     FFPVGGDGLLGEPHVVASAGPTPYGFAFAGGETLVVTEAFRAEKGAAAASSYALANGS
                     AAAVSRSVGTGRSEICWAVATADGRFAYATNFADGAVSRYAVGADGRLTLEDASAGVA
                     VEGAAGLRDEDLSRDGRFLYAIDADARRIFGWSVGADGELRLVGSWDGIPATVAGLAA
                     S"
     gene            complement(12804..13055)
                     /locus_tag="VFO88_08420"
     CDS             complement(12804..13055)
                     /locus_tag="VFO88_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545589.1"
                     /translation="MTRRQLNYLLLGLFALFMGVSIVAREVAGAAESRVAGGALALAT
                     AVVLLVLSRRGGDDARLRHLGAFLVVLGLVLLAVGIRGL"
     gene            complement(13092..14015)
                     /locus_tag="VFO88_08425"
     CDS             complement(13092..14015)
                     /locus_tag="VFO88_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9545590.1"
                     /translation="MPTLIVLVLVGFAAQLVDGALGMAYGATSTTLVLAAGYSPAVAS
                     ASVHVAELGTTLASGAAHWRFGNVDWRTVRRIGVPGALGAFAGAVVLSSISSELATPW
                     MAGVLLVLGVYVLLRFTLAGVPTRPGRRYVRGRFLAPLGLAAGFVDATGGGGWGPVAT
                     PTLLASGRMEPRKVVGSVDTSEFLVALAASAGFLLALGSQVLSLRIVAALLIGGLAAA
                     PLAAWLVRIVTPRLLGALVGGLIVVTNVRTLFTVADEPAAVRGWTYVALLAVWAGAIA
                     LVVRAHRRDGTPLIERRPGAVGSADALADAR"
     gene            14102..14515
                     /locus_tag="VFO88_08430"
     CDS             14102..14515
                     /locus_tag="VFO88_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9545591.1"
                     /translation="MWISRRTDYATRAVLALTLADGGPLKLEQIAETVEAPRSVLEQV
                     MPALVAAGVVRSVRGPAGGYRLNVAPDELTLERVVRVFQGQLAPIGYATRKNPDEAPE
                     LVERSLKETWAEVRDATIEILGRTTFADLAARATS"
     gene            complement(14546..14618)
                     /locus_tag="VFO88_08435"
     tRNA            complement(14546..14618)
                     /locus_tag="VFO88_08435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14583..14585),aa:Gly,seq:ccc)
BASE COUNT         1958 a         5549 c         5221 g         1958 t
ORIGIN      
        1 cacacggttg agcgccgtct tgcaggtgtc ctcgaggaac gagacgcgcc ccacgcggga
       61 agggtagacg aacgtacgtt cgtgcgcgaa ccggctacgc ggcctcgcgg agcgcctcga
      121 gctcgcggct caccgcgagc cgcgacagtg cctcctgctc gatctgccgc acccgctcgc
      181 gcgtgagccc gaggtcgcgg ccgatcgact cgagcgactg cgggtcctgc ccgccgagcc
      241 cgtagcgcag gcgcaggatg tcgcgcgcgc ggtcgggcag gtcgtcgagc gcctggcgaa
      301 gcgcctcctc ccggaggctc acgtcgatct cctcctccgg catgtcgctc tcgcctgcga
      361 agagctcgcc gagcgccgtc ccgtcgtcgt cgccgacggg cttgtcgagg ctcgtgaccg
      421 cacgcgccgc ggcgcgcacc tcgcgcacct gcttcacgct gaggcgcgcg gccttcgcga
      481 tctcctggtc ggtcgggtcg cgcccgagct tcacgacgag ctcgcgctcc gcccgcgcga
      541 tgcgctgctc gcgctcgacg atgtggacgg ggatccggat cgtccgcgac ttgttcgcca
      601 cgccgcgctg gacggcctgg cggatccacc acgtcgcgta cgtcgagaac ttgaagccct
      661 tccgccagtc gaacttctcg accgcgcgga tgagcccgat gatcccttcc tggatgaggt
      721 cgaggagcga gagcccgtgg ccctggtagc gcttcgcgat cgagacgacg aggcggaggt
      781 tcgcgttcac catctgctcc ttcgcctgct cgtcgccgcg ctcgatgcgc ttcgcgagcg
      841 ccacctcctc gtccgcggtc agaagcttgt gccgcccgac ctcgttcagg aagagctgga
      901 gcgcgtcggt cgtcgacgag gcgaggtcgc cgttcacgta ggtcggcgac gcgccctcgc
      961 ggccgcagtc gtcggtcacc tcgatcccgc gcgagtcgag ctcgtcgaag agcgcctcgg
     1021 tcgcgtcgtc gtcgaggccg agctcctgga ctagcgcggt cgcctcgccg aggttcacgc
     1081 agcccgcctc ctcgccgcga tcgaagagcg cctcgatctg cgcgcgcgcg acgtcgtcgt
     1141 gcgtctcgga atccgccatg cgtcaagcct ttcccgctgc gccgcgcgac taacgcaccg
     1201 gcgaaagccc tcctgtgggg gatgcaacgg cgcgcgacct cgccgacggt gggagagaac
     1261 cccgacggaa cgccctctgc ggccgggcct tccagacgct tcatggaacc cacgccgcgc
     1321 cgtctcactc gcacgctctt ccggatcgcc ttcgcggcgg cgctggcgct cgccgtgctc
     1381 acggccggtc ccgccggcgc cggccccgcc ggcccgacgc tcatcggccc gacggacggc
     1441 gccgcggtcg agatgctccc ggtcttcgcc tggcaggcgg tcacgaacgc cgaccgctac
     1501 gagctcgagc tctccgccga ccccggcttc aactccgcga tcgccacggt gagcacgcgc
     1561 aacacgcgcg cgacgctcaa gaccgcgatc gcgaacggga cgtactactg gcgcgtccgc
     1621 gccgtcaccg cggcgggcgc gctcggctcg tggtcggcct cccgcatcgt cgagatggcg
     1681 tggacggcga acccgtcgct cctctcgccc ggcgacggcg cgatcctcga ctacccgtcg
     1741 gacgcgctcc ggctctcgtg gacgcccgtc gagggcgcga gcgagtacct cgtgcgcatc
     1801 gcgaccgacc ccgcgctcgg ctccctcatc tgggcgagcg ggccggtgaa gacggccgcg
     1861 accggctaca ccctcaccgc gccgatcgcc cccgggacgt actactgggc gatcacgccg
     1921 ctcaacgccc agggccacgc cgggacgccg tcgagcacgc agtcgttcca gtggcggtgg
     1981 ccgtcgacga cgacgccgat cttcaccgac ctcgggccgc agccggagat catcgacccg
     2041 ttcttctcct ggccgcgcgt gcccggcgcc gcggggtacg aggtcgaggt caactcctcc
     2101 tccgactggg cgcccggctc gagggtctgc tgcgacgccg tccggttcgg gacgggcatc
     2161 tcgaccctcg ggctctcgct cgcgccgctc gtgcagctcg acaacaacac gtactactgg
     2221 cgtgtccgcc cgatcgaccc gagcgggaac gccggctcgt ggagcgtcgg cccgtcgttc
     2281 acgcagacct ttgcgaacac cccgccgacg cccgcgccga gcgtgaagaa cctccgcgtg
     2341 cgcgacaacg tcggcgaccc gtacgacggc ctcgacccgg cgtccgtccc gtacccgatc
     2401 tcgaccgccg cgcccgtcct gacgtggaac ccggtgcccg gcgcgtcctc gtaccaggtg
     2461 gacgtgaccc cgtacgaggg cggcgcctgc gactggagct acccgacgac cggccactgg
     2521 gtgaagacga cgaccacgaa cgcgtggacg ccgctcggct ggagctggaa caacgtgaag
     2581 ccgttcccga acgcggcgtc cgtctcgaac gagacgacct cgctcgtcgc cgggcgcacg
     2641 tactgcgcgc gcgtccgccc gcaggacggc gcctccgccg ccggcgggcc gctcgtgtcg
     2701 ggcgactgga cgtacctccc ggcgaacaac tcgccggcgt tcacgtggac cggctccccc
     2761 gccccgggcg agtgctcgtg cgagctcgcg gcgtccgact acaacacgcc ggtcacgggg
     2821 tcgacgctcg cgcggatgcc gctcttcacg tgggacgccg tccccggcgc gcagagctac
     2881 ttcgtcctcg tctcgcgcga cccgaacttc acgaacctcg tcgactacgc gttcacgaag
     2941 atcgccgcgt acgcgccgcg tacctcgaac cagacgaagg gctatccgga cgagctcacc
     3001 tcgtactact gggccgtcct ccccgcgcgt gacctcgacg ggagcggcgt gtccgcgaac
     3061 ccgctcgacg ggacgccgcc gtcgttcgag aagcaggcgg tcccgccgac gctcgtcgcg
     3121 ccggacccgg gcgcggtgct cgacgggccc acgaccttcc gctggacgaa cgccgaggcg
     3181 gcccgccgct accggatcca ggtctcgcag gacccgagct ttgcgaaccc gatcgacgac
     3241 acggtcaccg actcgaccgc gtacacgtcg aacacgacgt atcccgcgaa cacgacgctc
     3301 tactggcgcg tgcgcgccga cgcggagaac ggcaccggct acgtcgggct ccgctggtcg
     3361 gacacgggca cattcacgaa gcagttgccc gcgccggtcc ccgatccggg gaacccgacg
     3421 ggcggcgcgc tcatcccgac gtggagctgg gcgccggtcc cgggcgcggt ctcctacgac
     3481 gtcacggtcg aggagcccga cggcgaccgg cggaacttcg acgaccttcc cctccacgcg
     3541 ttcacgccgg tgaagatgac cggggtcggg atcttcaccg cccaggtgcg cgcgaacttc
     3601 ccgacggcca cgagccaggt cgtgcccggg ccgtactcgc cgctcatgcc cttcgcgcgg
     3661 acgattcccg agccgggcgg cgcggccgcg gacaagggcg cgaaccatct gctcttcgtc
     3721 tggaacccgc ggccgggcgc gaagcagtac cggctccagg tctccacgcg cgccgacttc
     3781 gcgctgccgg tcgagaccgt cctgacccag gcgccgaacc acgcgcccgt gctgacgcag
     3841 cgcgactaca tggacggcgg gacgttcttc tggcgcgtcg ccgcgatcga cgcggacggg
     3901 aacatcggcg actacagccc gacgcagcag gtcgcgacga cgcagcggat ccggctctcc
     3961 gcgtacggct ccccgtacgt gcggcgcaag accacgatca tcgtctcgac ccgcacgagc
     4021 cgcggcccga tcgccggcgt caacgtccgt ctctggggcg ccggcctgcg ggcgcggacg
     4081 aagcggacgg ccgccgacgg ccgcgtcacc ttcggcatca cgccgaggaa gcgcggcagg
     4141 atctacatcc gcgccacgaa ggccggctac ttcacgacgc agacgtcgat ccccgtccgc
     4201 cgccgctagc gatcatctag ctcgcgtact tgcctgccgg ccgcggtcgt ggtcaactgc
     4261 gggggtaccg gttgcaaccg tcaggattcc ggtgagcacg ccgcttcggc tctccggacg
     4321 aggtgcccgt ccgtgcgacg ggcgtgatcg cgagccgccg tcccgtccgg gccggtgctt
     4381 gcggggcctg gccgccgccg ccgcctcgct ctcccctcga gggggtccgg cggcggccca
     4441 ggccgtgctc ggacaagatg cgcctgcgtg ctcgcgctct tccccgacac cgccgacgcc
     4501 gccggtggag agctctcgct cggcggcgtg cccgtctccc ggctcgcggc cgggcacggg
     4561 acgccgctcg tcgtcctgtg cgcggagacg ctgagggccc gggcccgggc gtacctcgac
     4621 gcggctccgg acgcgctcgt cgcctacagc gtcaaggcct tcccgaacgt cgccgtgctg
     4681 cgggagctcg cggagctcgg cctcggggcc gacgtctcga cgctcgggga gctctccttc
     4741 gcgctcgcgg ccggcgtgcc gggcgaacgg atcgtcgtcc acggcaacaa caagtcggac
     4801 gaggagctgc gcgcggccgc ggaggcccgc gtgcgccacg tcgtgctgga ctcggaggac
     4861 gaggtcgagc gggcggccgc ggccggcgtc acccgggtgc tcgtgcgcgt gacgccgggg
     4921 atcgacgcgg acacgcacgc cgcgatccgg acggcgcacg ccggctcgaa gttcgggctt
     4981 ccgccggagg ccgcgctgcg ggcgatcgag gccgcgcggt cggccgggct cgacgtggcc
     5041 gggatccacg tccacatcgg ctcgcagctc ctcgacacgc agacggcgcg ctcgagcgtc
     5101 gactgggccg cgacgttcgc ggccgactgc cgcgccgagc tcggctggac gccgtccgtc
     5161 ctgaacgtcg gcggcgggct cgggatcgcg cacgtcgagg acgagcgccc gccttcgatc
     5221 ggggacttcg tcggcgcgct cgccgggcgg atccggcacg agtgggagct gctcggcctg
     5281 ccgctcccgt cgctcgtgct cgagccgggc cgctcgatcg tcggtcccgc gggcgtgacg
     5341 ctctaccggg tcggcgtcgt gaagcgggcg agcgagtcga ccacctgggt ggcggtcgac
     5401 ggagggatgt ccgacaaccc gcgcccggcg ctctacggcg cacggctgac ggcggtgctc
     5461 gccgagcgcc tggacgagga gcccgccggc ccgtacacgg tctgcggcaa gcactgcgag
     5521 tccggcgacg tcctcgtcga ccgggtcgcg ttaccggagc cgcggcgcgg cgacctcctg
     5581 gccgttccgg cgacgggcgc gtacacgctc gcgatggcgt cgacgtacaa cgcggtgccg
     5641 cgcccggccg cggtgctcgt cgagaagggc gaggcgcgcg tgatccgccg ccgcgagacg
     5701 gtcgccgagc tcctggccca cgagacctag tgacacagtg ttacttggac gccgcggcga
     5761 acgcctcgac ggtgcggtcg atctcctcgt ccccgtgcgc gagcgacacg aacatcgcct
     5821 cgtactggct gggcgcgacg tagacgccgc gctcgagcag ggcgcggaag accgcggcgt
     5881 agcggtcggt gtcgctcgcc tgggcctcgg cgaggttccg caccgcgccc tcgcgctgga
     5941 agaccgtcag catcgcgccg atgcgctgca ctgtcgcgcc cgtctcccgc aggccggcct
     6001 cgaggcgcgc gccgcggcgc tcgagctcct cgtagacggc cggatcccgc aggcgccgca
     6061 gcaccgagag cccggccgcc gtggcgagcg ggtttccgga gagcgtcccc gcctggtaga
     6121 cgtcgccggc cggcgcgagc ctctccatca cgtcgcgccg tccaccgaac gcgccgacgg
     6181 ggaggccgcc gccgaggatc ttcccgagga tcgtcaggtc cggccggacg ccgaaccgct
     6241 cctgcgcccc gccgcgcgcg acccggaagc ccgtgatcac ctcgtcgagg acgagcagcg
     6301 cgcccacgcg gtcgcacacg gcgcgcaggc cctcgaggaa gccgggctcc ggcgggacga
     6361 cgcccatgtt ccccgcgacc ggctcgacga gcacggccgc gagcgactcg tccgccgcct
     6421 cctccaccgc ggcgaggtcg ttgtagggcg cgacgagcgt gtccgcggtc accgcggacg
     6481 ggacgccggg cgtggacggg atcccgagcg tcgcgagccc cgagccggcc tccgcgagaa
     6541 gcgcgtccgc atgcccgtgg tagcagcccg cgaacttgag gatccggtcg cgcccggtcg
     6601 cagcgcgggc gaggcggatc gcgctcatcg ccgcctccgt cccggacgag acgagccgga
     6661 cgctctcgat cgacggcacc gcgtccgcga tctcctcggc gagctcgacc tcggcctcgg
     6721 tggacgcccc gaagctcgtc ccgcgccgcg cggcctcggt gaccgcggca accgtctccg
     6781 gatccgcgtg cccgaggagg agcggccccc acgacatgac ccagtcgacg tagcggcgcc
     6841 cgtccgcgtc cacgaggtac gcgccctcgc cggacgcggc gaagagcggc gcctcgaggc
     6901 cgaccgactt catcgcccgg acgggcgagt tcacgcctcc cgggatccgt gcgagcgcgc
     6961 ggcggtagag ctcgccgctc gcgctcaggc gcgctcctcg tcgagcgcga agagcttgcg
     7021 gagcgccgcc gcgtactcct cggcgccctc ggaggcggcc tccttcacgc gcaccgtcgg
     7081 ctcgtgcagg agcttgttca cgatctgcgc cgtgagcgcc tcgacctcgc ggcgttcctc
     7141 gtcggagagc gtgcgcaggc gcggcgcgac ccgctcgagc tcggcctcgc ggatctcgtc
     7201 ggcacggcgg cggagcgcgg tcacgaccgg gacgacctcg agcgacctca gccacgccgc
     7261 cagctcgccg gcgcccccgg ccgcgatccg ctcggcggcc tcccactgct cgtcgcggtg
     7321 tgcgagcgcg tcgcccgcct ctgggccgag cgcgtcgagg tcgtagagga tcgcgccgtc
     7381 gaggtcggcg atcgccgggt cgagatcgcg cgggacggcg atgtccacga gcaggagcgg
     7441 atggtcttcg cggccggcga gcgcctgctc cacctcgtcc gcgtggagga tcgtctctgg
     7501 acagcgcgtc gagctgagga cgacgtcggc gcgcgcgagc tgctcgccga tctgctcgaa
     7561 cgggacggcc tcgccgccga agcgcgcggc gagctgctcg gcccgctcga gccggtggtt
     7621 cgcgacgaag acccgctccg ctccgcgcgc gaggaagctc gtcgcggcca gctcgcccat
     7681 cttccccgcg ccgatcacga ggatccgctt gccgtcgagc ttgccgatcg tccgctccgc
     7741 gagcgcggcc gcggcggcgg ggatcgcggc ggggagcgtg ccgacaccgg tgtcggtgcg
     7801 cacctgcttc ccgcagtgga gcgcatgctc gaagaggcgg ttgaggactg ggccggtcgt
     7861 gccggcgccg gacgcgacgt cgtacgcctc gcgcacctgg ccgatgatct gcgcctcgcc
     7921 cgggagcagc gactcgagcc cggccgtcac gcggaacagg tggtgcgccg cctcggcgtc
     7981 gcggcgctcc tcgagcagcg actcgaggtc tgcttccgcg aggccggagc gccgggcgag
     8041 cgcaccgcgt gcgacggccg ccgcgagcgc cgcgtcgtgg ccggcgaggt agagctccgt
     8101 ccggttgcac gtcgagagga cggccgcctc gaacccttcg tcggcgagct gctgcgccac
     8161 ggcggcgagc tcgttcggcg gcacgtgcag cagctcgcgc acgtcgagcg cggcgctgtg
     8221 atgggagacc ccgacctgga cgagcgcagg cgagtgacgg ccgttcgagc ccacgccgtg
     8281 acgctaccac cctgcgcggc ggtccggcgc ccgagcgtgc gccggtgccg ggcaccggcc
     8341 gcgtcccgcc gagccgagcc cgcccgcggg aacgccctgc aaaccgcccc gttagggccg
     8401 aagaggccac ggctcccggc ggcacgtccg gtgccaggca ccgggcgtgg cccggcgaga
     8461 cgggttcgcc cgtccggggg agctacgcgc gccggtgcca gccgatgcaa cagccgtggc
     8521 ctacgacgtc accacacgcg cgggcggatg ggtcgccgct caggcggctc ggcgctggcg
     8581 gcgggcgagc ctcgtcggcg gcgtcgggct gctcgccacg atcgttctcg tcacctccac
     8641 gctggccggt cggacgcacc cgctgctcgt tctcggcgtg ctcgccgtcg cgctcgtcgc
     8701 ctatcggatc gcggactcga tgctcgacga cgccgtccac tggagggacg gcgcgcgggc
     8761 cgagggagcg gtcggcgacc tcctcgacac gctccggccg gagttcgtcg tcatgcacga
     8821 tctccgccag ctcggtgaag gcaacgtcga ccacctcgtg agcggcccga gcggcgtctt
     8881 catgatcgag acgaaggcgc gccgctaccc gccgaacgct ctccgcaagg cgaagcgcca
     8941 ggcggcgaag ctcggcaacg agctcgaagt ctgggtcacg ccggtgatct gcatccacga
     9001 gcgccgcggc agtgagccgt tcaaccatca cggcgtctgg atcgtcagca gtgaacggct
     9061 ccccgcctgg cttcgcacgc agcggaacgc gacactcccc ttcgagcggc tcgcgcgctt
     9121 cgcggactcg ctctgaaccg gcgggcggtt ctgttacaac gggcgcgtga cggagcggct
     9181 cgcacgcgcg tccgcgctgc acccggtgcg gacgctgctc gcctgggtcg gcgggatcgt
     9241 cctcgcgatc gtgctcgtgg ccatgctcct cggcgggaac ctcacgagcg aggggaacgt
     9301 cacgaacaac ccggagagcg tgcgcgcgaa cgcgatcctc gcggagtcgc tcccggtcga
     9361 ggacaccgcg agcgagctcg tcgtcgtgcg ctcgaacgac gtcctcatgg tcgacaccgc
     9421 cgtccggctc gccgtcgagg agatgctggc cgaggccggc gacgcggtcg agcaggccga
     9481 gctctggcac gagagccaga atccgacgct cctctcagag gaccgcgcgg cgctgctcgt
     9541 gccgatcgag ctccgcgcgc ccgaggagga gaccgtcgag gagctcgtcg ccgccgtcca
     9601 ggccgcggac gagcgcagcg agaacctgaa cttcgaggtg accggccgct acacgaccgg
     9661 cgccgacttc acggaggtgt cggagcgcga cctccaggaa ggcgagctcc gcttcggcct
     9721 cccgtccgcg ctcgtcgtgc tcgtcctcgt cttcggcgcg ctcgtcgcgg cactcatgcc
     9781 gctcgcgctc gcgctcgtct cgatcatcgt cgcgctcggc ctgaccgcgc tcgtcgccca
     9841 gcagtgggac ctctccgtct tcgtcgtgaa catgctcacg gggatgggcc tcgcgctcgg
     9901 gatcgactac gccctgttcg tcgtctcccg cttccgcgag gagcgcgcgc acggccggga
     9961 gaagctcgac gcgatcgtga agaccggctc gaccgcgagc cgcgccgtgc tcttcagcgg
    10021 taccgcgttc gtcttcgcca tgaccggcct cctgctcgtc cagagcacga tcatgcgcag
    10081 cctcgccgtc ggcgcgatcc tcgtcgggat cgtctcggtc gcggcggcgc tcaccctcct
    10141 ccccgcgctc ctcagcctgc tcggcgaccg cgtgaaccgg ctgcgcgtgc cgttcgtcgc
    10201 ccgcgagatc gagcgggaca gcgcgcagga gagccgcttc tggggctgga tcgtccagcg
    10261 cgtcatccgc cgccccgcgc tcagcttcgc cgtcgccgcc gccgcgctcc tcgccctcgc
    10321 cgtcccgctc ctcgggatga acatcggcgc cgccggaatc ggcacgctgc ccgacgcgct
    10381 cgtctcgaag caggggctcg tcgcgctcca ggactccttc ccgcgcgcgg gcgcggagcc
    10441 ggcgcgaatc gtcgtccagg gggacgtggc ctcgcccgag atcgagggcg cgttcgagcg
    10501 gctcgaggcg gagctcgccg aggacgggac gttcggcccg cccgtcctcg agcaggcgcc
    10561 cgacggcgac gtggccgtga tgaccgtgcc cgtcgcggcc gacgcgctct ccgaccaggc
    10621 gatcgacgcg gtggagcggc tgcggggcga ctacgtcccg gccgcgttcg agggcgtcga
    10681 ggccgagccg ctcgtcggcg gcacgaccgc gaacaacatc gactacttcg acgtgatgaa
    10741 gcggtggctc ccgctcgtct tcgtcttcgt gctcggcctg agcttcatcc tgctcaccgt
    10801 cgccttccgc tcgatcgtca tcgcggggac ggcgatcgtc ctgaacctcc tctcgatcgg
    10861 cgcgacgtac gggctgctcg tgctcgtctt ccagcacggg tggggcaccg acctgctcgg
    10921 cttccagcag gtggacatca tcgaggcctg ggtgccgctc ttcctcttcg cggtgctctt
    10981 cgggctctcg atggactacc aggtcttcct gctcagccgg atccgggagc gcttcacgca
    11041 gaccggctcg acgaacgacg cggtcgcctt cgggatcggc tcgacggcgc ggctcatcac
    11101 gggcgcggcg ctcatcatcg tcgccgtctt cgtcgggttc gcgatgggcg acctcgtgat
    11161 gttccagcag atgggcttcg ggatcgcggt cgcgctcctc atcgacgcga cggtcgtccg
    11221 ctcggtgctc gtcccggcgg cgatgacgct gctcgggagc tggaactggt acctccccgg
    11281 ctggctccag tggctgcccc acatggaggt cgagggccac acggactagc ggtccacgat
    11341 ctccgccacc tcgaggacga cgtcgacgcc cggcccgtcc tcgcgctcgc gcacctcgcc
    11401 ggtgagcgcg cacacggtgt cgttcagcca gcggtagcgc tcgtcgtcgc tcaggtgcgt
    11461 gatcgcgccg gcgagcctgc cgcgccggcc gtatccgtgc caggagaaga ggatcgtcgc
    11521 cccgtcgtcc gtctcgtaga cgccgcggaa gtcgggcgtg agcgtcccgt cgacccgacg
    11581 ccgcgggaag ttggccccgc ggaggcgcgc cgagaggcgc ccgtccaggc gcccctcggc
    11641 gatgaggaag gtctgcccct cggtgccgtg ctcgccggcg agctcgacgc tccagtgctc
    11701 tggggtcgag aacgtcgcgc ggtagagcgg ctcgaggcgc atcagctcgc ggcgaggccg
    11761 gcgacggtcg cggggatccc gtcccaggag ccgacgagcc ggagctcgcc gtcggccccc
    11821 acgctccatc cgaagatccg ccgcgcgtcg gcgtcgatcg cgtagaggaa gcggccgtcc
    11881 cgggagaggt cctcgtcccg caggccggcc gcgccctcga cggcgacgcc ggcggaggcg
    11941 tcctcgagcg tcaggcggcc gtccgccccg accgcgtagc gcgagaccgc gccgtcggcg
    12001 aagttcgtcg cgtacgcgaa gcgcccgtcg gcggtcgcga ccgcccagca gatctcgctc
    12061 cggccggtcc cgaccgagcg cgagaccgcg gccgcggagc cgttcgcgag ggcgtaggac
    12121 gacgccgccg cggcgccctt ctccgcccgg aacgcctcgg tgacgacgag cgtctcgccg
    12181 cccgcgaagg cgaagccgta cggcgtcggg cccgcggacg cgacgacgtg cggctcgccg
    12241 agcaggccgt ccccgccgac cgggaagaag gcgatcgagt cggtgccgcg gtccgtgacc
    12301 acgagcttcg agccgtctgg cgcgaagccg acctgcgcgg ggtcggagcc cgcggcgagc
    12361 gcccgctcgg agccggcgag cggctcgagc ccgccgtccg tcacgcggaa gcccgcgagc
    12421 gacggcatcc cggtgttcag gacgtagacg agacccccgt gctcggcgac gctcttcggc
    12481 gccgcaccgg tgggcacggc tgcgcgcagc tcgagcccgc cctcggcgac ggcgaagacg
    12541 ctgacgctgc cggagcccgc gttcgtcacg aagagccgcg agccgtcgcc actcaggacg
    12601 accgagccct gcgaggtgag gtgcggcacc ccgtcgcccg cgccgccggt cgcgtatgcg
    12661 ccgagcgggg cgagcgcgcc gtcgtcgccg cgccggaagg cgaggacgcc gttctcccgg
    12721 ccttcgttcg tctggacgta cacgttcatg tcggttcctc ctcgtcggtt gacgaggtgt
    12781 ccgtcccgtc tcgtgcgcgt tcgttacagg ccgcggatcc cgaccgcgag caggacgagc
    12841 ccgagcacga cgaggaacgc gccgaggtgc cgcagccgcg cgtcgtcgcc cccgcgccgc
    12901 gagaggacga ggaggacgac cgccgtcgcg agcgcgagcg ccccgccggc gacccgcgac
    12961 tccgcggcgc ccgcgacctc gcgcgcgacg atcgagacgc ccatgaagag ggcgaagagg
    13021 ccgaggagca ggtagttgag ctgccgtctc gtcatgcagc aggcagccgg atgcgggcga
    13081 gcgggagcgc gtcagcgagc gtcggccagc gcatcggccg accctaccgc gccgggccgc
    13141 cgctcgatca ggggggtgcc gtcccgccgg tgcgcgcgca cgacgagcgc gatcgcgccg
    13201 gcccagaccg cgaggagcgc gacgtacgtc cacccgcgaa cggccgccgg ctcgtccgcg
    13261 accgtgaaga gcgtccggac gttcgtgacg acgatcagcc ctccgacgag cgcgccgagc
    13321 aggcgcgggg tcacgatccg gacgagccag gccgcgagcg gcgcggctgc gagcccgccg
    13381 atcaggaggg cggcgacgat acggagcgag agcacctggc ttcccagcgc gaggaggaac
    13441 cccgcgctcg cggcgagcgc gacgaggaac tcgctcgtgt ccacggaccc caccaccttg
    13501 cgcggctcca tccggccgga ggcgaggagc gtcggcgtcg cgaccggccc ccagccgccg
    13561 ccgcccgtcg cgtcgacgaa gcccgcggcg agcccgagcg gcgcgaggaa gcggccgcgc
    13621 acgtaccgcc gtcccggtcg cgtcggcacc ccggcgagcg tgaagcggag gagcacgtag
    13681 acgccgagga cgagcaggac tcccgccatc cacggcgtcg ccagctcgga ggagatcgag
    13741 ctcagcacga ccgctccggc gaacgcgccg agcgcgccgg ggacgccgat gcgcctgacc
    13801 gtgcgccagt cgacgttccc gaagcgccag tgcgccgcgc cggaggcgag cgtggtcccg
    13861 agctcggcga cgtgaaccga cgcggaggcg acggccgggc tgtagccggc cgcgagcacg
    13921 agcgtcgtcg acgtggcgcc gtaggccatt ccgagcgccc cgtccacgag ctgggccgcg
    13981 aaaccgacga ggacgagcac gatcagcgtg ggcatattgt cggtaatcct tccctgttca
    14041 gtaagaatta gcggaaatga agtcgcggac ggtaccgggt tacggtgggc cctgtcaaac
    14101 gatgtggatc tcgcggagaa ccgactacgc gacccgagcc gtcctcgcgc tgacgctcgc
    14161 ggacggcggc ccgctgaagc tcgagcagat cgccgagacc gtcgaggcgc cccgctccgt
    14221 cctcgagcag gtcatgcccg ccctcgtggc ggcgggcgtc gtccgctccg tgcgcggccc
    14281 ggcgggcgga taccggctca acgtcgcacc ggacgagctg acgctggagc gcgtcgtccg
    14341 agtcttccag ggccagctcg ccccgatcgg ctacgcgacc cggaagaacc cggacgaggc
    14401 gcccgagctc gtcgagcgct ccctgaagga aacgtgggcg gaggtccggg atgcgacgat
    14461 cgagatcctc ggccggacga cgttcgccga cctcgccgcc cgcgctacct cctgacgcgc
    14521 acgagagccg cattcgggtc ggccaagcgg acgacggggc tcgaacccgc gaccctcagc
    14581 ttgggaagct gatgctctac caactgagct acgtccgcgt gcgggtcatt ctagcgaggg
    14641 ataggcgaat ccccggcccc gcccgacaat gagggcagaa aaaccg
//
LOCUS       DASTFC010000286         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_336606,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000286 DASTFC010000000
VERSION     DASTFC010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336606"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFO88_08440"
     CDS             complement(<1..858)
                     /locus_tag="VFO88_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET9545592.1"
                     /translation="MAITTPVEGGLGDHGLDPRGEIVWQPSTSQLYVHSLRAGTATLA
                     EGGPLVVDTGAHTGRSPDDRFVVREPGSEARIAWGDVNKPIEEEQFEGLRGKVVERLE
                     QGDVYVVNAFAGADPERRLAVRVVTYSAWHALFAKTLFIEPTDEELEGYVPQALVLHA
                     PDVLADPEEDGTRSETFVCLHPTRLEVVIGGTEYAGEIKKSIFTLMNDRLPLEGVFPM
                     HCSANVGPDGDVAVFFGLSGTGKTTLSADPERRLIGDDEHGWGGAGVFNFEGGCYAKM
                     IRLSPEAEPE"
     gene            952..1404
                     /locus_tag="VFO88_08445"
     CDS             952..1404
                     /locus_tag="VFO88_08445"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9545593.1"
                     /translation="MARELTLERTQVVPRTVDETFAFFAAPANLEALTPPWLRFRIVS
                     APVKLEAGSLLRYRLRLFGVPIRWLTEITAWHPPRGFTDLQLAGPYPLWEHSHRFTVV
                     EGGTEVYDVVRYRVPGGPLAPLVDRLLVARWLRDIFDYRRDRLTELLG"
     gene            complement(1385..1999)
                     /locus_tag="VFO88_08450"
     CDS             complement(1385..1999)
                     /locus_tag="VFO88_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET9545594.1"
                     /translation="MRLRLSVRRLVVASWPVSAEAVRRAVPPGLEPALVDGEHLASVV
                     AVRFAGGRLGRVPVFPFSQLNLRTYVEAGGEPAVLFLRSYVTPGGLGGILFGAPFRLA
                     RVRVREDAVRAPGAGIDLRFRPGGATDPGDLGRHEVGLYEAAGLRRFRVERGPAEWRS
                     AEPVGDARADGLLAVGLDVSGPPRIAVAGETWFETDVPPSRAAP"
     gene            complement(2048..2545)
                     /locus_tag="VFO88_08455"
     CDS             complement(2048..2545)
                     /locus_tag="VFO88_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545595.1"
                     /translation="MVKRLILLAGVAVLAAAFAAPAVAAEPVRDTIVQNNVVQGQTSC
                     GFLVWEIHLTAERFRFFDNDGNLVRVQVHIREDNTLTNVTTGETFREGPVSFMQTTIF
                     TDEGPRIVATGMAVNIFGQAFKDVGRVVINPFTGDIEFSAGPHPLREAQSEGNVLEGF
                     CGLWA"
BASE COUNT          360 a          979 c          936 g          356 t
ORIGIN      
        1 ctccggctcg gcctcgggcg agaggcggat catcttcgcg tagcagccgc cctcgaagtt
       61 gaagacgccg gccccgcccc acccgtgctc gtcgtcgccg atcaggcggc gctccgggtc
      121 ggccgagagc gtcgtcttcc cggtcccgga caggccgaag aagacggcca cgtcgccgtc
      181 ggggccgacg ttcgccgagc agtgcatggg gaagacgccc tcgagcggca gccggtcgtt
      241 catgagcgtg aagatcgact tcttgatctc gcccgcgtac tcggtgccgc cgatcacgac
      301 ctcgagccgc gtcgggtgga ggcagacgaa cgtctccgag cgcgtcccgt cctcctccgg
      361 gtcggcgagc acgtcgggcg cgtggaggac gagcgcctgc gggacgtagc cctcgagctc
      421 ctcgtcggtc ggctcgatga agagcgtctt cgcgaagagc gcgtgccagg ccgagtacgt
      481 gacgacgcgg acggcgaggc ggcgctccgg gtcggcgccc gcgaacgcgt tcacgacgta
      541 gacgtcgccc tgctcgaggc gctcgacgac cttcccgcga aggccctcga actgctcctc
      601 ctcgatcggc ttgttcacgt cgccccacgc gatccgcgcc tcggagccgg gctcgcgcac
      661 gacgaagcgg tcgtccggcg agcggccggt gtgcgcgccc gtgtcgacca cgagcggccc
      721 gccctccgcg agcgtggcgg ttcccgcgcg gagcgagtgg acgtagagct gcgacgtcga
      781 gggctgccag acgatctcgc cccgcgggtc gagcccgtgg tcgccgaggc ctccctctac
      841 cggtgtcgtg atcgccatga gtcgggtgcc ctctacccct gcttcggatg gagggaacgc
      901 cgacaatggt atgccgtccc gcaaggtggc gcgccgttac gctcgcgcgg gatggctcgc
      961 gagctcaccc tcgagcgcac gcaggtcgtg ccgcggacgg tcgacgagac gttcgcgttc
     1021 ttcgccgcgc ccgcgaacct cgaggcgctc acgccgccct ggctgcgctt ccggatcgtc
     1081 tccgcgcccg tgaagctcga ggccggctcg ctcctccgct accggctccg gctgttcggc
     1141 gtgccgatcc gctggctgac ggagatcacc gcctggcacc cgccgcgcgg gttcaccgac
     1201 ctccagctcg ccgggccgta cccgctctgg gagcactcgc accggttcac cgtggtcgag
     1261 ggcgggacgg aggtctacga cgtcgtccgc taccgcgtcc ccggcggccc gctcgcgccg
     1321 ctcgtcgacc ggctgctcgt cgcgcgctgg ctgcgcgaca tcttcgacta ccgccgcgac
     1381 cggctcacgg agctgctcgg ctaggaggga cgtccgtctc gaaccacgtc tcgcccgcga
     1441 cggcgatgcg cggcggcccg ctcacgtcga ggccgacggc gaggagcccg tccgcgcggg
     1501 cgtcgccgac gggctcggcg cttcgccact cggccgggcc gcgctcgacg cggaagcgcc
     1561 gcaggccggc cgcctcgtag agcccgacct cgtgccggcc gagatctccc gggtcggtcg
     1621 cgccgcccgg gcggaagcgc aggtcgatcc ccgcgccggg cgcacggacg gcgtcctcgc
     1681 gcacgcgcac gcgcgcgagg cgaaacggcg ccccgaagag gatccctccg agcccgccgg
     1741 gcgtgacgta cgagcggagg aagagcacgg ccggctcgcc gcccgcctcc acgtacgtgc
     1801 gcaggttgag ctgcgagaac gggaagacgg gcacgcgccc cagccgcccg ccggcgaagc
     1861 ggaccgcgac gaccgacgcg aggtgctccc cgtccacgag cgccggctcg aggcccggcg
     1921 gcacggcgcg ccgtacggcc tcggccgaga cgggccacga ggcgacgacg agccggcgga
     1981 cggagagccg aaggcgcacg gacgcgagtg tcgcacctcg cgcccgtgcg cccgacggtt
     2041 caggcgtcta cgcccagagg ccgcagaagc cctcgagcac gttgccctcc gactgggcct
     2101 cgcggagcgg gtgcgggccg gcggagaact cgatgtcgcc cgtgaacggg ttgatcacga
     2161 cgcggccgac gtccttgaac gcctggccga agatgttcac cgccatgccc gtcgcgacga
     2221 tccgcgggcc ctcgtccgtg aagatcgtcg tctgcatgaa cgagaccggg ccctcgcgga
     2281 acgtctcgcc ggtcgtcacg ttcgtgagcg tgttgtcctc gcggatgtgc acctggacgc
     2341 ggacgaggtt cccgtcgttg tcgaagaacc ggaaccgctc ggccgtcagg tggatctccc
     2401 agacgaggaa gccgcaggac gtctggccct gcacgacgtt gttctggacg atcgtgtccc
     2461 ggacgggctc ggccgccacg gcgggggccg cgaacgccgc cgcgagcacg gccacgcccg
     2521 ccagcaagat cagtcgcttc accatctttt tccctcctag tgtcggtccc gggaccgcaa
     2581 cgcggcaccg tagcggacac gcgcggcagc ttcaaatggg caggaacggc c
//
LOCUS       DASTFC010000287         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_188510,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000287 DASTFC010000000
VERSION     DASTFC010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188510"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFO88_08460"
     CDS             complement(<1..908)
                     /locus_tag="VFO88_08460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9545596.1"
                     /translation="MIVPDLSPEIRELKARARRFVEEELYPAEQRIVERGDVDEGEVE
                     ALRGKARAEGFSNYNLPPEHGGADLPMLAQVAIEEEAGRATNGLGFIVAERGPREILE
                     LATPDQMERFIGPLLRHETYEAWAITEPDAAGSDVAALEATAERDGDDWLLNGEKWYV
                     TGGQHASFFIVLAVAGGEQTLFFVQKDAPGLEIVRTPRFMHDPYIDRHLELRLTDCRV
                     PDANRVPSGGNEGAKRWFVVERLMIAARCCGAADRLLGLCRSWALEREAFGKPIAEFQ
                     GVAFPLADSLTELAAARLLTYHAAHA"
     gene            complement(918..1340)
                     /locus_tag="VFO88_08465"
     CDS             complement(918..1340)
                     /locus_tag="VFO88_08465"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9545597.1"
                     /translation="MSGPVRHDRRIIPHEKTIEIRWRDIDNFGHVNNAVYLTYLEECR
                     DEWVERAVGPDKSWDFVLARVAIDYRRELTQADEVVVARCRLEGLGTSSIRTREELVT
                     RDGEISAEAEAVLVARDPETARSRALTDEERASFERAS"
     gene            1320..3341
                     /locus_tag="VFO88_08470"
     CDS             1320..3341
                     /locus_tag="VFO88_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET9545598.1"
                     /translation="MADGAGQDELRGGIGPLLFPRSVAVVGASPRLPEPVVSVLRGGV
                     PAWGVNPRRADVEGLPCVPSVAELPETPEVAVLLVGHRAVEEAFEVAAAAGVRAFVVP
                     GLGNESGRDGPPVIARLARRAAELDAAVVGPNCMGVAVPDGPSPWLGIVPDTFVRGRV
                     SVVAQSGSVGEAFLALRRRVGFRCVVSSGGELSRDAADFLAFFAGDEETGAIGLFLET
                     VRRPGAFVAALARAAEAGKPVVCLKVGRSEAGARTALAHTGALVGSAEAFSAVLRAYG
                     VVEVHDFPELVETLEAFGRARARPRGARIGAVSESGGEGALLADHAEAAGLPFAPLPD
                     ELARAVQAEFPNYVSPGNPLDAWAVDEPERVYPRSLELMARSGAFDVLVALVDVSRYR
                     DETGQDWGNPALAALADGVEGTDVYPAVATVHTVDPPDHVLDLVRERDVALLRGPGAA
                     ARALAELVRWRPRRPPAPEGGVRVELADLLEGRGALPELESTEVVSRYGIPVVEALRA
                     SSPDEAADAAARLGPPVVVKVDGVAHKAAVGGVVLGVGTPEEAAEAAARLGGRVIVAR
                     QAAAGAEAFVGMTRDSDYGPILAVGRGGSSVERLGAGATALAPVDLATARELVAVAGV
                     EDVNDAVAGALVAVGRLAAEHPEIAEVDVNPLVLSPGGAVAVDALVVLA"
BASE COUNT          366 a         1316 c         1333 g          478 t
ORIGIN      
        1 aaggcgtgcg ccgcgtggta ggtgagcagg cgcgcggccg cgagctcggt gagcgagtcg
       61 gcgaggggga aggcgacgcc ctggaactcg gcgatcggct tgccgaacgc ctcccgctcg
      121 agcgcccagg agcggcagag cccgaggagc cggtcggccg ccccgcagca gcgcgcggcg
      181 atcatgagcc gctcgacgac gaaccaccgc ttcgccccct cgttcccgcc gctcgggacg
      241 cggttcgcgt ccggcacgcg gcaatcggtg agccggagct cgagatgccg gtcgatgtac
      301 gggtcgtgca tgaagcgggg cgtccgcacg atctcgagcc cgggcgcgtc cttctggacg
      361 aagaagagcg tctgctcgcc gcccgcgacc gcgagcacga tgaagaacga cgcgtgctgg
      421 ccgccggtca cgtaccactt ctcgccgttc aggagccagt cgtcgccgtc gcgctcggcc
      481 gtcgcctcga gcgcggcgac gtcggagccc gccgcgtccg gctcggtgat cgcccacgcc
      541 tcgtacgtct cgtggcgcag gagcgggccg atgaagcgct ccatctggtc gggcgtcgcg
      601 agctcgagga tctcgcgcgg cccgcgctcg gcgacgatga agccgagccc gttcgtcgcc
      661 cgcccggcct cctcctcgat cgccacctgc gcgagcatcg gcaggtcggc gccgccgtgc
      721 tcgggcggga ggttgtagtt cgagaacccc tcggcgcgcg ccttcccgcg cagcgcctcg
      781 acctcgccct cgtccacgtc gccgcgctcg acgatgcgct gctcggccgg atagagctcc
      841 tcctcgacga accgccgcgc ccgcgccttg agctcgcgga tctccgggga cagatccggg
      901 acgatcatcc gcgaatccta tgacgcgcgc tcgaagctcg cccgctcctc gtcggtgagc
      961 gcgcgtgagc gggccgtctc cgggtcgcgg gcgacgagca cggcctccgc ctcggcggaa
     1021 atctcgccgt cgcgcgtcac gagctcctcg cgcgtgcgga tgctcgacgt gccgagcccc
     1081 tccaggcggc agcgggcgac gaccacctcg tccgcctgcg tcagctcgcg gcggtagtcg
     1141 atcgcgacgc gcgcgaggac gaagtcccag ctcttgtccg gcccgacggc tcgctcgacc
     1201 cattcgtccc ggcactcctc gaggtacgtg aggtagaccg cgttgttcac gtggccgaag
     1261 ttgtcgatgt cgcgccagcg gatctcgatc gtcttctcgt gcgggatgat gcgccgatca
     1321 tggcggacgg ggccggacag gacgagctgc gcggcgggat cgggccgctc ctcttcccac
     1381 gttcggtcgc ggtcgtcggc gcctcgccgc gcctgccgga gccggtcgtc agcgtcctgc
     1441 gcggcggcgt gcccgcctgg ggcgtgaacc cgcgccgcgc cgacgtcgag gggcttccgt
     1501 gcgtgccgag cgtcgcggag ctccccgaga cgccggaggt cgcggtgctg ctcgtcgggc
     1561 accgcgcggt cgaggaggcg ttcgaagtgg cggcggccgc cggcgtgcgc gccttcgtcg
     1621 tgccggggct cgggaacgag tcggggcggg acgggccgcc ggtgatcgcc cgcctcgcgc
     1681 ggcgcgcggc ggagctcgac gccgcggtcg tggggccgaa ctgcatgggc gtggccgttc
     1741 cggacggccc ctcgccgtgg ctcgggatcg tgccggacac gttcgtccgc gggcgcgtgt
     1801 cggtcgtcgc gcagtcgggc tcggtcgggg aggcgttcct cgcgctgcgc cgccgcgtcg
     1861 ggttccgctg cgtcgtctcg tccggcgggg agctctcgcg cgacgccgcc gacttcctcg
     1921 ccttcttcgc cggcgacgag gagacgggcg cgatcgggct cttcctcgag acggtgcgcc
     1981 ggccgggcgc gttcgtcgcc gccctcgcgc gcgccgcgga ggcggggaag ccggtcgtct
     2041 gcctcaaggt cggccgctcc gaggcgggcg cgcgcaccgc gctcgcgcac accggcgcgc
     2101 tcgtcggctc ggccgaggcg ttctcggcgg tgctccgcgc gtacggcgtg gtcgaggtgc
     2161 acgacttccc cgagctcgtg gagacgctcg aggccttcgg gcgtgcgcgc gcccgcccgc
     2221 gcggcgcgcg gatcggggcg gtctccgagt cgggcggcga gggcgcgctt ctcgccgacc
     2281 acgccgaggc cgccggcctg ccgttcgcgc cgctcccgga cgagctcgcc cgcgcggtgc
     2341 aggccgagtt cccgaactac gtctcgccgg gaaaccccct cgacgcctgg gccgtggacg
     2401 agccggagcg cgtctacccg cgctcgctcg agctcatggc ccgctcgggc gcgttcgacg
     2461 tgctcgtggc gctcgtggac gtctcgcgct accgcgacga gaccgggcag gactggggca
     2521 accccgcgct cgccgcgctc gccgacggcg tcgagggaac ggacgtctac ccggcggtcg
     2581 cgacagtcca cacggtcgac ccgcccgacc acgtgctcga cctcgtgcgc gagcgcgatg
     2641 tcgccctcct gcgcggcccg ggcgccgcgg cccgcgcgct cgcggagctc gtccgctggc
     2701 ggccgcgccg cccgcccgcg ccggagggcg gcgtccgggt cgagctcgcg gacctgctcg
     2761 agggccgggg cgcgctgccg gagctcgagt cgaccgaggt cgtctcgcgc tacgggatcc
     2821 cggtcgtcga ggcgctccgg gcgtcgtcgc ccgacgaggc ggcggatgcc gcggcccgcc
     2881 tcggcccgcc ggtcgtcgtg aaggtggacg gcgtcgcgca caaggccgcc gtgggcggcg
     2941 tcgtgctcgg cgtcggcacg ccggaggagg ccgccgaggc tgcggcccgg ctcggcggac
     3001 gcgtgatcgt cgcccgccag gccgccgcgg gcgcggaggc gttcgtcggc atgacccgag
     3061 actccgacta cgggccgatc ctcgccgtcg gtcgcggcgg ctcgtccgtc gagcgcctcg
     3121 gcgcgggcgc gacggcgctc gcgcccgtcg acctcgcgac cgcgcgggag ctcgtggccg
     3181 tcgccggggt cgaggacgtg aacgacgccg tcgccggcgc gctcgtcgcg gtcgggcgcc
     3241 tcgcggccga gcaccctgag atcgccgagg tggacgtgaa cccgctcgtc ctctcgcccg
     3301 gcggcgcggt cgccgtggac gcgctcgtcg tgctcgcctg agcggttccc caccgcgccg
     3361 ccgccgcgtc cggcatgatg cgccgcacga cggcgacgga gggagttgat cgagcatgag
     3421 cactcgtcat ccccggcagc cggcggggct caaccgccgg gagttcttgc agcggtcggc
     3481 cggaggcgcc atc
//
LOCUS       DASTFC010000288         8039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_600087,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000288 DASTFC010000000
VERSION     DASTFC010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600087"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFO88_08475"
     CDS             <1..658
                     /locus_tag="VFO88_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9545599.1"
                     /translation="LASIGLVYGSLLAFRAPDVRGVVAYSSLGQMCLIVIGLLAFNDS
                     GLTGAVLHMVSHGLVSAGLFLLAGTIERRTATDELALLGGMARGRPRLATLLITLSVI
                     ALAVPFSVAFAGEFLILNGIFAVAWGWAVVGAAAIVLAALYMLRLVSAVLHLDQGEAV
                     NEVQLDLRPAEVGIVGTLAVVLVALSFWPAAITDRVFGGEPARAVETGFAGRGGEASP
                     "
     gene            655..2175
                     /locus_tag="VFO88_08480"
     CDS             655..2175
                     /locus_tag="VFO88_08480"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9545600.1"
                     /translation="MTEPLRHPDIDWAALAPVLTLLGTACFVLLVGVFLPRGVRRPFA
                     AFFAAAGLVAAGIVSGILFALDDEATTLVRDSLARDRLAELGQIFVAGAGLLAVGVAY
                     FESRGPRGDDPRVAEDDWRERVVEWYVLLLTAVAGMAFFVQANSLITLFLGLEWFSIA
                     LYVLCAINVHRVAALEAGLKYLIVGSFGSAILLFGSAFVYGGAGGLTFGEIASGAADG
                     ERLFLVAGLAMILVGLAFKVSAAPFHMWTPDVYEGAPTPVTAFMSAATKIAALVLTLR
                     LLLTAFPGEEELWTIAIAVLATISLAWGNIAALTQRSVKRLLAYSSVSHAGFLLMPIA
                     AGNALGGRALLFYLVPYAALSIGSFAVVAARERELAADVTLDNLAGFGWERPFHAIAM
                     AVFMFGFIGLPPAGLFLGKFYVFTAVYERGWTWLVIVGVVATVVSVAYYLAVVRAMYS
                     GSAELRTAPAGGSPPRDWPLTGTVLACLVLAVATLVVPDPLVDLARDSLAFLSFPL"
     gene            2351..3682
                     /locus_tag="VFO88_08485"
     CDS             2351..3682
                     /locus_tag="VFO88_08485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9545601.1"
                     /translation="MSRVVVLGGGIAGESFVAALAREAPDADVTLVERELVGGECSYW
                     ACIPSKTLLRPVELAAAVARTPGVSGARIDPEGVFAWRDAIAGGGDASQVKWVENLGA
                     AFVRGDAEIAEAGLVRAGGRELPYDHLLVATGSDPATPPLDGLEDVRPWTSRDATSAS
                     EVPERLLVVGGGAVGCELAQLYARLGSEIVLVQSGATLLDRIDADAGALLAECLAEDG
                     VEVRTGTRPSAVETAGGRVRLLCDGAGAGELEGTEVLLAVGRRPRVEGYGLDRAGVEL
                     ERNAVKTDDRLAAADGVWAAGDVTGKALFTHVGKYQARIAAANVAGGERRADYRAIPA
                     AIFTDPQIASVGDTSGKDGAVTATAELATVSRAGTYSDPTPRGFVKLFADPGRGVLVG
                     AVVVGPEAGEMIGQLTVAVRADVPVDVLRDTIQPFPTFSEAVFFAARDLPL"
     gene            3694..4110
                     /locus_tag="VFO88_08490"
     CDS             3694..4110
                     /locus_tag="VFO88_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9545602.1"
                     /translation="MGNGADTVTLGRLALFADLSRPQVEAIAHTFEEEMFPEGQRVLR
                     QGFSGSNLYVILDGEAAVVIDGKERTRLGRGEFFGEASTLLGTAPTADVVATTGLRCL
                     VVPASELEGFLMAYPAVAIRMLREVVQRLATTLQWL"
     gene            4143..5576
                     /locus_tag="VFO88_08495"
     CDS             4143..5576
                     /locus_tag="VFO88_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9545603.1"
                     /translation="MGSGPGGLQVSYCLSRLGIEHAVLSADDAPGGMFRSLPIFGRLL
                     SWSKPDAPFERGSREYEWFDHNSLLADDPELRGLVPAEMNRDFAVPSRPEVERGLAAF
                     AERAGVHVRYGCRWETTRREEDGSLVLGTSEGEYRCRAAVFALGVTDPWKSDIPGVED
                     VPHYVETLEPRAYEGKRVVVIGKRNSGFEIADGLLPWARQVVLVSPRPVQTSVIALSS
                     VRVRYMQPLEDAGLGGGTLALDAAIERVERTADGFRIHAYGTTRPGELELEADAAIAA
                     TGFATPIRDLGQLGVQTVAQGRIPALTPFWEAVGAPGVYFAGNATQGAAGLRRHGVGA
                     SSPAVHGFRYNARVLAEAIAERHFGVVPERATFAPGEVARFLAHELAHGPELWAQKAY
                     LARVVALEEGGAGRDAGVQPLAHFVDAAGPDAVAATIEMNAAGSIYPVVYVRRGGTVA
                     EHVLEPEPQNAYDGPGYLAELEGLLRS"
     gene            5573..6961
                     /locus_tag="VFO88_08500"
     CDS             5573..6961
                     /locus_tag="VFO88_08500"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9545604.1"
                     /translation="MSPRALAWAALVALPLAALVLLLAVPRLDGLWQHNPSHFWLVLL
                     AAVTNVVLAVVTGDAARRRGDARLFLVSLAFLSAAGFLALHALATPRVLLDTPNAGFV
                     VATPVGLLLASAFAAASSLELSPETSVRVMRRASQLRIGLFVLLAAWAAVSLAGIPPL
                     HDPLPEESARGWLLAFALPGVALYAFAAWRYLGLYRRRRAPLLLYVVTAYALLAEAMI
                     AIAAGRDWHASWWEWHLLMLVAFGLVAWSARREWHEERFADLYLDATAGTVRRVSVLF
                     ADLEGFTAYAEQAGPERVSAVLEEYLAVAIPAVREHGGETDKLIGDALMAVFNKRGDQ
                     PDHAERAVRAALALQRATGPVARAHPDWPRFRAGVNTGAAHVGVVGAAGSREHTALGD
                     AVNLASRLEGQARAGEVVIGPETYAELPDGTEVEPLGGVHVKGKAAPVEAYVVVSLPD
                     RNAGAGSGTADG"
     gene            6958..7200
                     /locus_tag="VFO88_08505"
     CDS             6958..7200
                     /locus_tag="VFO88_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9545605.1"
                     /translation="MTRELSCADLVELVTDYLEGRLPAAERARFEAHLGECPGCVAHL
                     EQARAAVALAGRLEERDLSPAARDALVAAFRDWKAR"
     gene            complement(7197..7703)
                     /locus_tag="VFO88_08510"
     CDS             complement(7197..7703)
                     /locus_tag="VFO88_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545606.1"
                     /translation="MAALVKAYKFLAGDGSGLFTRFRWPLPAGDAPGAWVEAEPVSSC
                     RSGVHACRVEQLPYWLAAGLYEVELEGEIVEEPLKVVAARGRLVRRIDAWNDAARAEF
                     AAACGERARELGGAALPDWPPPAGGEPPPPLAAFMAARIAERLGGIEAYRAERERQSA
                     WLAERLGL"
     gene            complement(7688..7951)
                     /locus_tag="VFO88_08515"
     CDS             complement(7688..7951)
                     /locus_tag="VFO88_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9545607.1"
                     /translation="MPEPRGTHEHNYTCEEVVELATEYVEGAMGPEETTLFEFHLNFC
                     DGCETFVDQIRASAGAAGRLGAEDVPADVRERLLEAFREWRRS"
BASE COUNT          873 a         3001 c         2982 g         1183 t
ORIGIN      
        1 gctcgcctcg atcggcctcg tctacggctc gttgctcgcc ttccgcgcgc cggacgtccg
       61 cggcgtcgtc gcctactcga gcctcggcca gatgtgcctg atcgtgatcg ggctccttgc
      121 gttcaacgac tcggggctca ccggcgccgt gctccacatg gtgagccacg ggctcgtctc
      181 ggccgggctc ttcctcctcg cgggcacgat cgagcggcga acggcgacgg acgagctggc
      241 gctcctcggc ggaatggcgc gtgggcggcc gcgactcgcg acgctcctca tcaccctctc
      301 ggtgatcgcg ctcgcggtgc cgttctcggt cgcgttcgcg ggcgagttcc tgatcctgaa
      361 cgggatcttc gcggtcgcgt ggggctgggc ggtcgtcggc gcggccgcga tcgtcctcgc
      421 cgcgctctac atgctcaggc tcgtctcggc cgtcctccac ctcgaccagg gcgaggcggt
      481 caacgaggtc cagctcgacc tgcgcccggc cgaggtcggg atcgtgggca cgctcgccgt
      541 cgtgctcgtc gcgctctcgt tctggccggc ggcgatcacc gaccgcgtct tcggcggcga
      601 gccggcgcgg gcggtcgaga ccggcttcgc ggggcgaggc ggtgaggcgt cgccgtgacg
      661 gagccgctcc ggcacccgga catcgactgg gccgcgctcg cgcccgtcct gacgctcctc
      721 ggcaccgcgt gcttcgtcct cctcgtcggc gtcttcctcc cgcgcggcgt gcggcggccg
      781 ttcgcggcgt tcttcgccgc ggcggggctc gtcgcggccg ggatcgtctc cgggatcctc
      841 ttcgcgctcg acgacgaggc gacgacgctc gtccgcgact cgctcgcgcg cgaccggctc
      901 gcggagctcg ggcagatctt cgtcgcgggc gcagggctcc tcgcggtcgg cgtcgcgtac
      961 ttcgagtcgc gcggcccgcg cggcgacgac ccgcgcgtcg ccgaggacga ctggcgcgag
     1021 cgcgtcgtcg agtggtacgt gctcctcctc accgcggtcg ccgggatggc gttcttcgtc
     1081 caggcgaact ccctcatcac gctcttcctc gggctcgagt ggttctcgat cgccctctac
     1141 gtcctctgcg cgatcaacgt ccaccgggtc gccgcgctcg aggccgggct caagtacctg
     1201 atcgtcggca gcttcggctc ggcgatcctg ctcttcggct cggcgttcgt ctacggcggc
     1261 gccggaggcc tgaccttcgg ggagatcgcc tcgggcgccg ccgacggcga gcggctgttc
     1321 ctcgtcgcgg gcctcgcgat gatcctcgtc gggctcgcgt tcaaggtctc ggccgcgccg
     1381 ttccacatgt ggacgccgga cgtctacgag ggcgcaccga cgccggtgac ggcgttcatg
     1441 tccgcggcga cgaagatcgc ggcgctcgtc ctgacgctgc ggctgctcct gaccgcgttc
     1501 ccgggcgagg aggagctctg gacgatcgcg atcgccgtcc tcgcgaccat ctcgctcgcg
     1561 tgggggaaca tcgcggcgct gacgcagcgg agcgtgaagc ggctgctcgc gtactcgagc
     1621 gtctcgcacg ccggcttcct gctcatgccg atcgcggccg ggaacgcgct cggcgggcgc
     1681 gccctcctct tctacctcgt gccgtacgcg gcgctctcga tcggctcgtt cgccgtagtc
     1741 gcggcgcggg agcgagagct cgcggccgac gtgacgctcg acaacctcgc cggcttcggc
     1801 tgggagcggc cgttccacgc gatcgcgatg gccgtcttca tgttcggctt catcgggctg
     1861 ccgccggcgg ggctcttcct cgggaagttc tacgtcttca cggcggtcta cgagcgcggc
     1921 tggacgtggc tcgtgatcgt cggcgtcgtc gccacggtcg tctcggtcgc gtactacctg
     1981 gccgtcgtcc gggcgatgta ctccggctcg gcggagctcc gcacggcgcc cgccggcggc
     2041 tcgccgccgc gcgactggcc gctcacgggg acggtgctcg cgtgcctcgt cctcgcggtg
     2101 gcgacgctcg tcgtgccgga tccgctcgtc gacctcgcgc gcgactccct cgccttcctc
     2161 agcttcccct tgtaggtgag cgcggctcag tgctcccccc ggcaatgatc agatcgtctc
     2221 cgccggcgtc cggacccgcc gtgccgcgtc ctcagtcgcc ccgatatgcc cgcatatcga
     2281 ggctccctgc gtccttgctc ggcggccccg gccggtcgcc ggagacgggt gacaatcacc
     2341 gggaggagca ctgagccgcg ttgtcgtcct cggcgggggg atcgcgggcg agagcttcgt
     2401 cgcggcgctc gcgcgggagg cgcccgacgc cgacgtcacc ctcgtcgagc gcgagctcgt
     2461 cggaggcgag tgctcgtact gggcgtgcat cccctcgaag acgctcctgc ggcccgtcga
     2521 gctggccgcc gccgtcgcgc ggacgcccgg cgtgagcggc gcgcgaatcg atcccgaggg
     2581 cgtcttcgcc tggcgtgacg cgatcgccgg cggcggcgac gcgagccagg tgaagtgggt
     2641 cgagaacctc ggcgcggcgt tcgtccgcgg cgacgcggag atcgccgagg ccgggctcgt
     2701 gcgggccggc gggcgcgagc tcccgtacga ccacctgctc gtcgcgaccg gctccgaccc
     2761 tgccacgccg ccgctcgacg ggctcgagga cgtccgcccg tggacgagcc gggacgccac
     2821 gagcgcgtcc gaggtgcccg agcggctgct cgtcgtgggc ggcggcgccg tcgggtgcga
     2881 gctcgcgcag ctctacgccc ggctcggctc cgagatcgtc ctcgtccaga gcggggcgac
     2941 gctcctcgac cggatcgacg ccgacgcggg cgcgctgctc gcggagtgcc tggccgagga
     3001 cggcgtcgag gtccggaccg gcacgcgccc gagcgcggtc gagacggccg gcggccgcgt
     3061 ccggctcctc tgcgacgggg ccggcgcggg cgagctcgag ggcaccgagg tcctgctcgc
     3121 cgtcgggcgg cgcccgcgcg tcgagggcta cgggctcgac cgcgcgggcg tcgagctcga
     3181 gcggaacgcg gtcaagacgg acgaccgcct cgccgcggcc gacggcgtct gggcggcggg
     3241 cgacgtgacc ggcaaggcgc tcttcacgca cgtcgggaag taccaggcgc ggatcgcggc
     3301 cgcgaacgtc gcgggcggcg agcgccgcgc cgactaccgc gcgatccccg cggcgatctt
     3361 caccgacccg cagatcgcgt ccgtcggaga cacgtccggg aaggacggcg ccgtgacggc
     3421 cacggccgag ctcgcgacgg tctcccgcgc gggcacgtac tccgacccga cgccccgcgg
     3481 gttcgtgaag ctcttcgccg accccggccg cggcgtgctc gtcggcgcgg tcgtcgtcgg
     3541 gcccgaggcg ggggagatga tcgggcagct caccgttgcc gtgcgcgccg acgtgccggt
     3601 cgacgtgctc cgcgacacga tccagccctt cccgacgttc tccgaggccg tgttcttcgc
     3661 ggcccgcgac ctgccgttgt agcctcccag ggaatgggaa acggggcgga tacggtgacg
     3721 ctcggccgtc tggcgctctt cgccgacctc tcgcgcccac aggtcgaggc gatcgcgcac
     3781 acgttcgagg aggagatgtt cccggagggc cagcgggtcc tccggcaggg cttctccggc
     3841 tcgaacctct acgtgatcct cgacggcgag gcggcggtcg tgatcgacgg caaggagcgg
     3901 acgcggctcg ggcgcgggga gttcttcggc gaggcgtcga cgctgctcgg cacggcgccg
     3961 accgcggacg tcgtcgcgac gaccgggctc cggtgccttg tcgtcccggc gtccgagctc
     4021 gaggggttcc tcatggcgta tcccgcggtc gccatccgga tgctgaggga agttgtgcag
     4081 aggctggcca ccacgctcca gtggctctga cccatccgcc gggcgaatat cccgtcgtgg
     4141 tcgtgggcag cggcccgggc gggctgcagg tgagctactg cctctcccgg ctggggatcg
     4201 agcacgccgt gctctcggcc gacgacgcgc cgggcgggat gttccgctcg ctcccgatct
     4261 tcggacgcct tctctcgtgg tcgaagcccg acgcgccctt cgagcgcggc tcgcgcgagt
     4321 acgagtggtt cgaccacaac agcctcctcg ccgacgaccc ggagctccgc gggctcgtcc
     4381 cggcggagat gaaccgcgac ttcgccgtgc cctcgcggcc ggaggtggag cgcggtctcg
     4441 cggcgttcgc ggagcgcgcg ggcgtgcacg tccggtacgg ctgccgctgg gagacgaccc
     4501 ggcgcgagga ggacggatcg ctcgtcctcg gcacgagcga gggcgagtac cgctgccgcg
     4561 ccgccgtctt cgcgctcggc gtcaccgacc cgtggaagtc ggacatcccc ggcgtcgagg
     4621 acgtgccgca ctacgtcgag acgctcgagc cgcgcgcgta cgagggaaag cgcgtcgtcg
     4681 tgatcggcaa gcggaactcg ggcttcgaga tcgcggacgg gctgctcccc tgggcgcggc
     4741 aggtcgtgct cgtctcgccg cggcccgtgc agacgtcggt catcgcgctc tcgagcgtgc
     4801 gcgtgcgcta catgcagccg ctcgaggacg ccggcctcgg cggcgggacg ctcgcgctcg
     4861 acgcggcgat cgagcgggtg gagcggaccg cggacgggtt ccgcatccac gcctacggga
     4921 cgacccgccc cggagagctc gagctcgagg ccgacgcggc gatcgccgcg accggcttcg
     4981 cgacgccgat ccgcgacctc gggcagctcg gggtccagac ggtcgcgcag gggcggatcc
     5041 cggcgctcac gccgttctgg gaggcggtcg gcgcgccggg cgtctacttc gccgggaacg
     5101 cgacgcaggg cgcggccggg ctgcgccggc acggcgtcgg ggcgagctcg ccggcggtgc
     5161 acgggttccg ctacaacgcg cgcgtcctcg ccgaggcgat cgccgagcgg cacttcggcg
     5221 tcgtgccgga gcgggcgacg ttcgccccgg gggaggtcgc ccggttcctc gcccacgagc
     5281 tcgcccacgg gccggagctc tgggcgcaga aggcgtacct cgcccgggtg gtcgccctcg
     5341 aggagggtgg cgcagggcgc gacgcgggcg tgcagccgct cgcgcacttc gtggacgcgg
     5401 ccggcccgga cgcggtcgcc gccacgatcg agatgaacgc ggccggctcg atctacccgg
     5461 tcgtctacgt gcggcgcggc ggcacggtgg cagagcacgt gctcgagccc gagccgcaga
     5521 acgcctacga cgggccgggg tacctcgcgg agctcgaggg gctgctccgg tcgtgagccc
     5581 ccgtgcgctc gcctgggccg cgctcgtcgc gctcccgctc gcggcgctcg tcctgctcct
     5641 cgcggtgccg cgcctcgacg ggctgtggca gcacaacccg tcccacttct ggctcgtcct
     5701 cctcgccgcg gtcacgaacg tcgtcctcgc ggtcgtgacc ggagatgctg cgcgccggcg
     5761 cggcgacgcg cggctgttcc tcgtctcgct cgccttcctc tccgcggccg gctttctcgc
     5821 cctccacgcg ctcgcgacgc cgcgcgtgct cctcgacacg ccgaacgccg ggttcgtcgt
     5881 cgcgacgccc gtcgggctcc tgctcgcctc ggcgttcgcg gccgcgtcgt cgctcgagct
     5941 ctcgcccgag acctccgtcc gcgtcatgcg ccgggcgtcg cagctccgga tcgggctctt
     6001 cgtcctcctc gccgcctggg cggccgtctc gctcgccggg atcccgccgc tccacgaccc
     6061 gctgccggag gagtcggcgc gcggctggct cctcgcgttc gcgctcccgg gcgtagcgct
     6121 ctacgcgttc gcggcgtggc gctacctcgg gctttaccgg cggcggcggg cgccgctcct
     6181 cctctacgtc gtgaccgcct acgcgctcct cgccgaggcg atgatcgcga tcgccgcggg
     6241 acgcgactgg cacgcgagct ggtgggagtg gcacctgctc atgctggtcg cgttcgggct
     6301 tgtcgcctgg agcgcgcggc gggagtggca cgaggagcgc ttcgccgatc tctacctcga
     6361 cgcgaccgcg gggacggtgc ggcgggtcag cgtcctcttc gccgacctcg aggggttcac
     6421 cgcgtacgcc gagcaggccg ggcccgagcg cgtctcggcc gtcctcgagg agtacctcgc
     6481 ggtcgcgatc cccgcggtgc gcgagcacgg cggcgagacg gacaagctga tcggcgacgc
     6541 gctcatggcc gtcttcaaca agcgcggcga ccagccggac cacgcggagc gggccgtgcg
     6601 cgcggcgctc gcgctccagc gggcgacggg gcccgtcgcg cgggcgcatc cggactggcc
     6661 gcgctttcgc gccggggtga acaccggcgc ggcgcacgtc ggcgtagtgg gcgccgcagg
     6721 cagtcgcgag cacacggcgc tcggcgacgc ggtgaacctc gcgtcgcgcc tcgaggggca
     6781 ggcgcgcgcc ggcgaggtgg tgatcggccc ggagacctac gcggagcttc ccgacggcac
     6841 cgaggtcgag ccgctcggcg gcgtgcacgt caagggcaag gcggcgccgg tcgaggccta
     6901 cgtggtcgtc tcgctcccgg accgtaacgc gggcgcgggt tccgggaccg cagacggatg
     6961 accagggagc tctcctgcgc ggatctcgtc gagctcgtga ccgactatct cgagggacgg
     7021 ctgccggccg ccgagcgcgc ccgcttcgag gcgcacctcg gcgagtgtcc cggctgcgtc
     7081 gctcacctgg agcaggcgcg ggccgccgtc gcgctcgcag gccggctgga ggagcgggac
     7141 ctctcgcccg ccgcccggga cgccctcgtc gccgcattcc gggactggaa ggcgcgctag
     7201 agcccgagcc gctcggcgag ccaggcgctc tggcgctccc gctccgcgcg gtaggcctcg
     7261 atcccgccga gccgttcggc gatccgcgcg gccatgaagg cggcgagcgg cggcggcggc
     7321 tcgcccccgg cgggcggcgg ccagtcgggg agcgccgcgc ctccgagctc gcgcgcccgc
     7381 tcgccgcagg cggcggcgaa ctcggcgcgc gcggcgtcgt tccaggcgtc gatccgccgg
     7441 acgagccgcc cgcgggcggc cacgaccttg agcggctcct cgacgatctc cccctcgagc
     7501 tcgacctcgt agagtccggc tgcgagccag taggggagct gctccacccg gcaggcgtgc
     7561 accccggaac ggcacgagga gacgggctcc gcctccaccc aggcgccggg cgcgtcgccg
     7621 gcgggaagcg gccagcggaa gcgcgtgaag aggcccgacc cgtcgcccgc gaggaacttg
     7681 tacgccttca cgagcgccgc cactcccgga acgcctcgag gaggcgctcg cggacgtcgg
     7741 ccggcacgtc ctcggcgccg agccgtccgg ccgctccggc cgacgcgcgg atctggtcga
     7801 cgaacgtctc gcacccgtcg cagaagttca ggtggaactc gaagagcgtc gtctcctccg
     7861 ggcccatcgc gccctcgacg tactcggtcg cgagctcgac gacctcctcg cacgtgtagt
     7921 tgtgctcgtg cgtgccgcgc ggctcaggca tcgaggtgct cctccaggcg tgcccgtacc
     7981 ttcgcccgcg cccggtgcag gaggacgcgc tggttcccct cggtgagccc gagcgccgc
//
LOCUS       DASTFC010000289         6459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_452016,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000289 DASTFC010000000
VERSION     DASTFC010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452016"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFO88_08520"
     CDS             <1..342
                     /locus_tag="VFO88_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545608.1"
                     /translation="APLHQHVDPRRVSVAHDVDHRLLDHAVERRLDRRRETLLAAAPS
                     GNRIFATLRGSVPLTGDPHVATGSTPGVGVFRVEQGGRAGTFLAVARIANIDAAGLDR
                     ADPHALAVRGG"
     gene            526..1062
                     /locus_tag="VFO88_08525"
     CDS             526..1062
                     /locus_tag="VFO88_08525"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9545609.1"
                     /translation="MRIAVLGGGRVGSALAATWQAAGHDVAVSTRDTVGEVAAGADVL
                     VLAVPAPAGPEVLAAAGPLDGRILVDATNNLSGGPSGAELAALVPGARVVKAFNTVFA
                     TFMHATPPERPASLVLCGDDAGAKEVVAGLVRDAGFDPVDAGGAERTGDVEAFARLVI
                     NLAYAQGRGPFVYRFEPH"
     gene            1117..2598
                     /locus_tag="VFO88_08530"
     CDS             1117..2598
                     /locus_tag="VFO88_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9545610.1"
                     /translation="MGHTLRNRLAAGGLAVLLVAGAIAGAVVALRAGSTTVERFALGT
                     VAVQVTPSRQGEVDAYAPLVDWGARVRPYRAPLDVRLEFRALDRDAALASLRSGDAAA
                     ENVRTVQRDLDGVVRDALAHAAIVGGLGSLAGGILAGALVAAVSRRRRWLAHGAFAGV
                     FFALAAISAAALDLRHADPAAFREPTFYGRGDELPQLLAFSEQLLTASERYTESYDTA
                     LRSLANVLAVGSGGLADGRERTTAIVASDLHTNGLVLPALRDFARDRTLFFVGDYTQL
                     GTRLENGIALGVSAAGGRVVAVSGNHDSAAFMRSLAEHGTLVLTRDGRLAADGSTDGE
                     TVVEVDGLPVAGWDDALESRGTLAEHRLDLDPALLLGQEELFVSWFESLEPRPSIVLV
                     HRHALAHALLEHVAADEDAPPLVILTGHDHEQHFHQEGPHVVVDGGTVGAGGPFEVGE
                     RPAGFAQLHVGPTGRLEALDLIEIEPLSGEASARRVALDPAAP"
     gene            2685..4235
                     /locus_tag="VFO88_08535"
     CDS             2685..4235
                     /locus_tag="VFO88_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9545611.1"
                     /translation="MPTLLARKPAAPPSPTPPAPEPPLPPPPRPWDDPRARALATVVA
                     AASLGGLAALVFPRGPVTTASALTLLALGLAVGLAAGALLRSRWAMLLAPLGFATAFE
                     LRWIPLESPVLDPPRFDTAYGILAALLGRGFFFLVAIVPMLVGVALGAAYARGRPERV
                     SAWRWARRALTAAAALGVAALVVALALPASTPPVPGGIAALEQIELGGDEQWISVRGT
                     SPDNPVLLYLSGGPGQTDMPQVRAWWRDLEEDFTIVNWDELGVGKSYPALGPGEEVTL
                     DRIVSDAIELAEKMRDRFGEEKVYLVGESWGSFLGVLVARERPDLFHAYVGSGQMVDI
                     LETDTRLYEDMLAYAERTGDEAAAERMRGYGPPPYDDPFANAYVMTYYEELAGEYDFL
                     PYLEERAAEVNPGPWGVFGSEYTLVEKVNVLRGLVDYFTVVYPQAQGIDLREEAGRLE
                     VPVYLVQGRHELDARTDLARSWFAGLEAPRKRFEWFENAGHATAFEEFRRFHDLMTET
                     VLAETYEP"
     gene            4485..5600
                     /locus_tag="VFO88_08540"
     CDS             4485..5600
                     /locus_tag="VFO88_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9545612.1"
                     /translation="MSFAGLIVKNLFRQRVRTGLTVLGIAIGITTVVALGVITAGFKE
                     ASGAFVQAGGADFAVAQKGASDLTFSAVSDEDRRAIAARPDVARATGFLLDVADVGGN
                     PYFFVFGYDPADLADEPLELVDGRLLAPGAPDEAVLGIEAAADLKKGVGDAVTLDGTR
                     FRVVGVARFDDDWRSAGALAPLATVQANASKRDVVTAVHVTAAQGLDPREVAAAIERD
                     LPELAAIVDVADYAEVDQGFKIMDAAELAISLLAVGIGAIGVMNTMIMSVFERTREIG
                     VLRAVGWRGSRILRMILYESLFLCLVAAVIGIALGLLASRAVLFVPAVSTFLTPGYPP
                     EVFVRALAVGVVVALAGAVYPAARAVRLSPMEALRHE"
     gene            5671..6300
                     /locus_tag="VFO88_08545"
     CDS             5671..6300
                     /locus_tag="VFO88_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9545613.1"
                     /translation="MDFDVAEGEFIAIVGPSGCGKSTLLHLLAALDRPDAGSLRVAHH
                     DLTAGRDLSHYRARHVGMVFQLHNLLPSLTASENVQIPLFELDLGGRERRARAQELLE
                     LVGLGGREGNRPPELSGGERQRVAIARALANDPAILLADEPTGSLDSAAGARVLDLLE
                     RLRRERGLTVVLVTHDAGVAARADRIVRMLDGRVDLRPAPAPEAVVSTA"
BASE COUNT          748 a         2470 c         2355 g          886 t
ORIGIN      
        1 gcgccgctcc accaacacgt cgacccgcgt cgcgtgagcg tggcgcacga cgttgatcac
       61 cgcctcctgg accacgcggt agagcgccgt ctcgaccgcc gccgggagac gctgctcgcc
      121 gccgccccgt ccgggaaccg gatcttcgcc acgctgcgcg gctccgtgcc cctcaccggc
      181 gacccgcacg tcgcgacggg ctcgaccccg ggcgtcggcg tcttccgcgt cgagcagggc
      241 ggccgggccg ggacgttcct cgcggtcgcg cgcatcgcga acatcgacgc ggccgggctc
      301 gaccgggccg atccgcacgc tctcgcggtg cgcggcggct gaggagccgg ttgcgcgggc
      361 acggccgatg ccataacttc acttccgaaa gcaagccgga ctcgttggcc gcgggctctc
      421 cgcgcaacag gcgaccttac gccgtccggt gccgggcgac tccccggggg ccggcagcgg
      481 ccggcccccc gcccggctcc cttcccgtct cgtaagctcg gggccgtgcg catcgcggtg
      541 ctcggaggag ggcgtgtcgg gagcgcgctc gcggccacgt ggcaggcggc gggccacgac
      601 gtcgccgtct cgacgcgcga cacggtcggc gaggtcgcgg ccggcgcgga cgtgctcgtc
      661 ctcgccgtgc ccgcgccggc cgggcccgag gtgctcgccg cggccgggcc gctcgacggg
      721 cgcatcctcg tcgacgcgac gaacaacctg agcggcgggc cgagcggcgc cgagctcgcg
      781 gcgctcgttc ccggcgcccg cgtcgtgaag gcgttcaaca ccgtgttcgc cacgttcatg
      841 cacgcgacgc cgccggagcg gcccgcctcc ctcgtcctct gcggcgacga cgccggggcg
      901 aaggaggtcg tcgccggcct cgtgcgcgac gccggcttcg acccggtcga cgcgggcggc
      961 gccgagcgca cgggcgacgt cgaggcgttc gcaaggctcg tgatcaacct cgcctacgcg
     1021 cagggccgcg gcccgttcgt ctaccgcttc gagccgcact agccgcggta cgatcggtgc
     1081 tgtcggcgcg tcccccggcc gccgatatcc gaggagatgg gtcacaccct gcggaaccgc
     1141 ctggcggcgg gagggctcgc cgtcctgctc gtggccggcg cgatcgccgg ggcggtcgtc
     1201 gcgctccgcg ccggctcgac gacggtcgag cgcttcgcgc tcgggacggt ggccgtgcag
     1261 gtgacgccct cgcggcaggg cgaggtggac gcatacgcgc ccctcgtcga ctggggcgcg
     1321 cgcgtgcgcc cgtaccgcgc gccgctcgac gtccggctcg agttcagggc gctcgaccgc
     1381 gacgcggcgc tcgcctccct ccgctccggc gacgcggccg cggagaacgt ccggacggtg
     1441 cagcgcgatc tcgacggcgt cgtccgcgac gccctcgccc acgcggcgat cgtcggaggg
     1501 ctcgggtcgc tcgccggcgg gatcctcgcc ggcgcgctcg tcgcggccgt ctcgcgccgg
     1561 cgccgctggc tcgcgcacgg ggcgttcgcg ggcgtcttct tcgcgctcgc cgcgatctcg
     1621 gccgccgcgc tcgacctccg ccacgccgac ccggccgcct tccgggagcc gacgttctac
     1681 ggccgcgggg acgagctgcc gcagctcctc gccttctccg agcagctcct gacggcgagc
     1741 gagcgctaca cggagtcgta cgacacggcg ctccggtcgc ttgcgaacgt gctcgcggtc
     1801 ggcagcggcg ggctcgccga cggccgggag cgcacgaccg cgatcgtcgc ctccgacctg
     1861 cacacgaacg ggctcgtcct ccccgccctg cgggacttcg cacgggatcg cacgctcttc
     1921 ttcgtcggcg actacacgca gctcggcacg cgcctcgaga acgggatcgc gctcggcgtg
     1981 tcggcggcgg gcggccgcgt cgtggccgtg tccggaaacc acgactcggc cgcgttcatg
     2041 cgctcgctcg ccgagcacgg gacgctcgtg ctgacccgcg acgggcggct cgccgcggac
     2101 ggctcgaccg acggggagac ggtggtcgag gtggacggcc tcccggtcgc gggctgggac
     2161 gacgcgctcg agagccgcgg gacgctcgcc gagcaccggc tcgacctcga tcccgcgctc
     2221 ctcctcggcc aggaggagct cttcgtctcc tggttcgaga gcctcgagcc gcggccctcg
     2281 atcgtgctcg tccaccggca tgcgctcgcg cacgcgctcc tcgagcacgt ggccgccgac
     2341 gaggacgcgc cgccgctcgt catcctcacc ggccacgacc acgagcagca cttccaccag
     2401 gaggggccgc acgtcgtcgt ggacggaggc accgtcgggg cgggcggccc gttcgaggtc
     2461 ggcgagcggc cggccgggtt cgcgcagctc catgtcggcc cgacggggcg gctcgaggcg
     2521 ctcgacctga tcgagatcga gccgctctcc ggcgaggcga gcgcgcgccg cgtcgcgctc
     2581 gacccggcgg ctccgtagtt cccgcgcgga ggtccgcggc cgcaccgatg cccgcggtcg
     2641 gcacgggacg gagacttcgg gcggaaccga ctgaaggagg cggcatgccc accctgctcg
     2701 cacgaaagcc cgccgcgcct ccttccccga cgccgccggc accggagccg ccgctgccgc
     2761 cgccgccccg cccctgggac gacccgcgcg cccgtgcgct cgccaccgtc gtcgccgcgg
     2821 cgagcctcgg cggcctcgcc gcgctcgtct tcccccgcgg gcccgtgacg acggcctcgg
     2881 cgctgacgct gctcgcgctc ggtctcgccg tcggcctcgc cgccggcgcg ctcctgcgct
     2941 cgcgctgggc gatgctgctc gcgccgctcg gcttcgcgac tgcgttcgag ctgcgctgga
     3001 tcccgctcga gagcccggtg ctcgacccgc cgcgcttcga cacggcgtac gggatcctgg
     3061 cggcgctcct cggccgcggc ttcttcttcc tcgtcgcgat cgtgccgatg ctcgtcggcg
     3121 tcgcgctcgg cgccgcgtac gcgcgcggcc ggccggagcg ggtctcggcc tggcgctggg
     3181 cccgccgcgc gcttacggcg gcggccgcgc tcggcgtcgc ggcgctcgtc gtcgcgctcg
     3241 cgctcccggc gagcacgcca ccggtccccg gcgggatcgc ggcgctcgag cagatcgagc
     3301 tcggcggcga cgagcagtgg atctccgtcc gcgggacgag cccggacaac cccgttctcc
     3361 tctacctgag cggcggtccc ggccagacgg acatgccgca ggtgcgtgcg tggtggcgcg
     3421 acctcgaaga ggacttcacg atcgtgaact gggacgagct cggcgtcgga aagtcgtacc
     3481 ccgcgctcgg gccgggcgag gaggtcacgc tcgaccggat cgtctcggac gcgatcgagc
     3541 tcgccgagaa gatgcgcgac cggttcggcg aggagaaggt ctacctcgtc ggcgagtcgt
     3601 ggggctcctt cctcggcgtc ctcgtcgcgc gcgagcggcc cgacctcttc cacgcgtacg
     3661 tcgggagcgg ccagatggtc gacatcctcg agacggacac gcggctctac gaggacatgc
     3721 tcgcgtacgc cgagcggacg ggcgacgagg cggccgccga gcggatgcgc ggctacgggc
     3781 cgccgcccta cgacgacccc ttcgcgaacg cctacgtcat gacgtactac gaggagctgg
     3841 cgggcgagta cgacttcctg ccgtacctcg aggagcgtgc cgccgaggtg aaccccgggc
     3901 cctggggcgt cttcgggagc gagtacacgc tcgtcgagaa ggtgaacgtg ctccgcgggc
     3961 tcgtcgacta cttcacggtc gtctacccgc aggcgcaggg gatcgacctg cgcgaggagg
     4021 cgggccggct cgaggtgccc gtgtacctcg tccagggacg gcacgagctc gacgcgcgga
     4081 cggatctcgc ccggtcctgg ttcgcgggcc tcgaggcgcc gcggaagcgg ttcgagtggt
     4141 tcgagaacgc gggccacgcg acggccttcg aggagttccg ccgcttccac gacctcatga
     4201 cggagacggt gctcgccgag acgtacgagc cctagaacgt cgccggccgt cgcccggagc
     4261 cggtccgcgc cccctttggg gggacgaagc accccagcac gaacgctacc gaggcgatcc
     4321 gcgcgcttcg tgccggtttc gtgccggatg tgtgaacggc agccggtcga gccgcgtcgc
     4381 gcccgcgaag gctccggctc gccgccagag atccgggaac tcgcggaggc ccggcccgtg
     4441 aactccccgt cgcgcgccca cgtacggcgc gcgacgcttg ggacgtgtcc ttcgccggac
     4501 tgatcgtgaa gaacctgttc cggcagcgcg tccggacggg gctcaccgtg ctcggcatcg
     4561 ccatcgggat cacgaccgtc gtcgcgctcg gcgtcatcac ggcgggcttc aaggaggcct
     4621 cgggcgcctt cgtgcaggcg ggcggtgccg acttcgcggt cgcccagaag ggcgcgtcgg
     4681 atctcacctt cagcgccgtc tccgacgagg accggcgcgc gatcgcggcc cggccggacg
     4741 tggcgcgcgc aaccggcttc ctgctcgacg tcgcggacgt gggcgggaat ccctatttct
     4801 tcgtcttcgg ctacgacccg gccgacctcg cggacgagcc gctcgagctc gtcgacggcc
     4861 gcctcctcgc ccccggcgcg cccgacgagg ccgtgctggg catcgaggcg gccgccgacc
     4921 tgaagaaggg cgtgggcgac gccgtgacgc tcgacggcac gcggttccgc gtcgtcgggg
     4981 tcgcgcggtt cgacgacgac tggcgcagcg ccggcgcgct cgcgcccctc gcgaccgtgc
     5041 aggcgaacgc ctcgaaacgg gacgtcgtca cggccgtcca cgtgaccgcc gcgcagggcc
     5101 tcgacccgcg ggaggtggcg gcggcgatcg agcgcgatct ccccgagctc gccgcgatcg
     5161 tcgacgtggc cgactacgcg gaggtcgacc agggcttcaa gatcatggac gccgccgagc
     5221 tcgcgatctc gctcctcgcc gtggggatcg gggcgatcgg cgtcatgaac acgatgatca
     5281 tgtccgtctt cgagcggacg cgcgagatcg gcgtcctgcg cgcggtcggg tggcgcgggt
     5341 cgcggatcct gcggatgatc ctctacgagt cgctcttcct ctgcctcgtc gccgccgtca
     5401 tcggcatcgc gctcgggctg ctcgcgagcc gtgccgtcct gttcgtcccc gccgtctcga
     5461 cgttcctgac gccgggctac ccgccggagg tcttcgtccg cgcgctcgcc gtgggcgtcg
     5521 tcgtcgcgct cgccggcgcc gtctatccgg ccgcccgggc cgtccgcctc tcgccgatgg
     5581 aggcgctccg ccatgagtag cgatcccttg atcgcagtcc gcggcctcga gaaaggctac
     5641 gagcacggtc gaatcacggc gctccgcgga atggacttcg acgtcgcgga gggcgagttc
     5701 atcgccatcg tcgggccctc cgggtgcggg aagtcgacac tcctgcacct gctcgccgcg
     5761 ctcgaccggc cggacgccgg ctcgctccgc gtcgcccacc acgacctgac ggccggccgc
     5821 gacctgagcc actaccgcgc gcgacacgtc gggatggtct tccagctgca caacctcctc
     5881 ccctcgctga cggcgagcga gaacgtccag atcccgctgt tcgagctcga cctcggcgga
     5941 cgggagcggc gggcgcgagc gcaggagctc ctggagctcg tcggcctcgg cggccgggag
     6001 gggaaccgcc cgccggagct ctcgggcggc gagcggcagc gcgtcgcgat cgcccgggcg
     6061 ctcgcgaacg acccggcgat cctcctcgcg gacgagccca ccgggagcct cgactcggcc
     6121 gccggcgcgc gcgtcctcga tctgctcgaa cggctgcggc gcgagcgcgg gctcacggtc
     6181 gtcctcgtca cccacgacgc cggcgtcgcg gcacgcgccg accggatcgt ccgcatgctg
     6241 gacgggcggg tcgacctccg cccggcgccg gcaccggagg cggtcgtctc gacggcctga
     6301 ccgtcgccta agtacttgac cgcagccggt ttccgtgcgg tcgacgatgc cgcggcgccg
     6361 ggcccgtgcg agcctcgctg catgagacct gtgctcgaac gggcggcacg gcgggcggcg
     6421 gccgtcgcgt ggcaggtcgt gctcgtcacg gtcgcggcg
//
LOCUS       DASTFC010000290         7602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1424698,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000290 DASTFC010000000
VERSION     DASTFC010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424698"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFO88_08550"
     CDS             complement(<1..452)
                     /locus_tag="VFO88_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545614.1"
                     /translation="MLERTKTGLREMPSNAVWLLSQAVKPAEALGDAAAGARDQGRKM
                     TAAVVDAAPVGDSVEIRARRAHDAAERAREAEERAVEAARDAKARADRAREVSERGHA
                     RVKDVERETSREVKQRVAEAQKAAEEYVRRERQAAEADAEDERQEVEA"
     gene            complement(565..978)
                     /locus_tag="VFO88_08555"
     CDS             complement(565..978)
                     /locus_tag="VFO88_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545615.1"
                     /translation="MIAVAECEDGLWLVEVGATAGDDALAGREPGGRVERPRPADLAP
                     AWVSSLLVDVDAAGSAVVVCVDRRPPLLVSHDAGTTWTERGSGLPPGRAVALGETPDD
                     VLYASRNRLHVSRDGGVFWRALAVELPEIRDAAWG"
     gene            complement(975..2306)
                     /locus_tag="VFO88_08560"
     CDS             complement(975..2306)
                     /locus_tag="VFO88_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HET9545616.1"
                     /translation="MATEGSWGVTPVPERLRTLGTLDLTLLWGNLGVSLLVLVTAAFL
                     VPALSIPEALLALVVGSLIGNLMLGTAGLIGADARVPAMVLMRGPLGRRGSYGATGLN
                     VAQCLGWATFEIVIIAAAASALSDRLFGVRAEAAWTVATGAVALSLALLGPIGVVRRV
                     IRKFAVWIVLASLVYLTWWALDGADLRALWDAPGEGGTTFVGAVDLVIAITVSWIPLA
                     ADYTRFGRTRRAAFVGTGGGYFLAATWMFVLGGLLVLAKGLSDPAEIPAGVAAAGLAS
                     ALALLAVTVDEADEAFANVYSTAVSLQNLVPRASQRVLLAGVAAVATLGALTIELSSY
                     ETFLLLLGSFFVPLFGVLLADWLLSGASYDGRDLLAAPAFRAGPVGAWIAGFALYQWL
                     HPLGPSWWVELVERTGPPETQIGSSLPSFALAFALAAAAILGERRLRPEPA"
     regulatory      complement(2295..2407)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2411..3019)
                     /locus_tag="VFO88_08565"
     CDS             complement(2411..3019)
                     /locus_tag="VFO88_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9545617.1"
                     /translation="MIEEHVVKEEVAHEPPPPRNPWLWLALLAVAVVAAAIIFVAVLA
                     DRASDDDDAAGPATTETVVVAPATAVVVPELVGFDHADAGRAAEDLALVANTYPVESD
                     APAGQVVAQEPEAGVELDTDERLRLDVSLGPDPLVAIMVPDVTGAEGAQAREILRRAG
                     FTVLTVDEDAPTPDEVGQVLRQDPAAGTEAPALTQVTVYTAR"
     gene            complement(3016..3624)
                     /locus_tag="VFO88_08570"
     CDS             complement(3016..3624)
                     /locus_tag="VFO88_08570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9545618.1"
                     /translation="MDRLFVVARHAESTLNAERRVNGDPSVPVGLTEKGREESRQLGV
                     QLAGLPLEACVVTRFLRTRETAELALEGRGVPLHEEPLLDDIRVGDLDGKTIEEYRAW
                     KHGNARSVPFPGGESLDDAAARYAEGFARLLASPWETTLVVCHEIPLRYLLNAAGGSH
                     ELDGPVHELRNATPYLFDEDGLARGVEGLARLTRGEAGSHQA"
     gene            complement(3624..4733)
                     /locus_tag="VFO88_08575"
     CDS             complement(3624..4733)
                     /locus_tag="VFO88_08575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9545619.1"
                     /translation="MTDESAEVVIVGGGAIGASTAFHLTELGVTDVVLVERATLASGS
                     TGKSAGGIRAQFADELNVRIALRSIAEFERFEALTGRPIDYEQSGYLFLLDREEDVAR
                     FRDAVALQRGLGVPTRELSVEEALSIVPQLSPEGLVGATFGPVDGRATAEAVVAGYAE
                     AAARRGVRIRQGEAVERIEVAGGRIAAVETTRARIATGAVVCAAGVWSPEVARLAGVE
                     LPVTGERRWIHFTPHSGGLPRPLPLTIDFSTGFYFHTEGPGLAFGGREQTLDELAGPA
                     TRRLPALADLPVQSSWSGFYEISPDHNAIVGEASEPGRFLYATGFSGHGFQQSPAVGE
                     HLAELVAGRVPTLDLSPFALERFARGTAREEAFVI"
     gene            4775..5086
                     /locus_tag="VFO88_08580"
     CDS             4775..5086
                     /locus_tag="VFO88_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9545620.1"
                     /translation="MHRFEVESVRRRLEEGNGGYEIVHASPGLEVGVYVLVAPEPDRQ
                     QPHEDDEVYVGLEGHGVLEVAGESVAIGPGEAIFVEAGVDHRFTAYESLSVLVVFTRP
                     A"
     gene            5083..6027
                     /locus_tag="VFO88_08585"
     CDS             5083..6027
                     /locus_tag="VFO88_08585"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9545621.1"
                     /translation="MNVLVTGATGFLGRHLCAALVERGDAVRAFVRPGTDAGALEALG
                     VEVVRGSLADAGALRAAAAGRALVFNAVGLVSYEWRQLDRLREANVESVRRVLAAVEP
                     GARLVHVSSGAAVGPSGSPDRLADEAQPFPEVARRNPYSVTKHDAEELVFAAVAGGLD
                     AVVGNPAIVLGPGDVYGSSTWPVRRYLQGTLRFHTRGGGASFVDVRDAAAGLVALAER
                     GRTGERYILTCREGNLPWNVFFRRVGAATGVRRVMLPLPPPVLRAAVRVVPWPVKPGE
                     ARTATEWWIFDPGKAERELGFTTRPLAETIRDTAADGR"
     gene            complement(6061..6471)
                     /locus_tag="VFO88_08590"
     CDS             complement(6061..6471)
                     /locus_tag="VFO88_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545622.1"
                     /translation="MAGEPEPRYRVARIEDVPALASDLVEGEWRPVRYHFGITAFGCN
                     AYVAREAGELIVEEHDEGEDGHEELYVVVSGVARFTLDGEERDAAGGTLVFCERPVVR
                     KAVAVEPGTTVLAIGGFPGRAFEVSRWESSRTGP"
     gene            complement(6474..7550)
                     /locus_tag="VFO88_08595"
     CDS             complement(6474..7550)
                     /locus_tag="VFO88_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET9545623.1"
                     /translation="MNYAFAGSPTSPVFEEFARELGVCLEERGFERAEGAGALEADLV
                     VNFIDPAAPKPFRRRSRGTFVAAIHEQQAVPDDVLKVNYPLLVRALANIVLCFVPEKG
                     VWFTTMERGHYGVDATDGVAELAEGVVERLAPLALSKLVIDNEFRTDLEEELWEGDEV
                     TDEIAEAGKRMGDLDLLPAPFPIEELLDEQDLRHVKRLYGIGGLSYGNLSQRKDETRF
                     WMSASGVDKTKLDIPGRDILLVSGYAPERNTMIMSVPPNVEPRRVSVDAIEHWMIYRA
                     HPDVGAILHVHAWMQDIPATDVNYPCGTEELATSVAQLLDAEPDPAHAAIGLRNHGIT
                     VTGESLTEILDRIEPKVLRQIPMS"
BASE COUNT         1004 a         2782 c         2847 g          969 t
ORIGIN      
        1 tccgcctcga cctcttgccg ctcgtcttcc gcgtccgcct cggcggcctg gcgctcgcgc
       61 ctgacgtact cctcggcggc cttctgcgcc tcggcgacgc gctgcttgac ctcccggctc
      121 gtctcgcgtt cgacgtcctt cacgcgggcg tgaccgcgct cgctcacctc gcgcgcgcgg
      181 tcggcacgcg ccttcgcgtc ccgcgcggct tcgaccgcac gctcctcggc ctctcgagca
      241 cgctccgcgg cgtcgtgcgc gcgcctcgcg cggatctcga cggagtcgcc gacaggcgct
      301 gcgtcgacca cggccgccgt catcttgcgg ccctggtcgc gcgcaccggc tgcagcatct
      361 ccgagcgcct cggccggctt caccgcctgg gacaggagcc aaacggcgtt cgacggcatc
      421 tcacgtaaac cagttttcgt tcgctccaac attcgacatc ccctccggac ggagaaacag
      481 gcaagttccc aactgcgccc cgcgttcccc gccgggtcgt gatcaaacca ggctcggggc
      541 cgtgagcagg ccctgccggg agcgtcagcc ccaggcggcg tcgcggatct ccggcagctc
      601 gaccgcgagc gcgcgccaga agacgccgcc gtcgcgggag acgtgcaggc ggttccggct
      661 cgcgtagagg acgtcgtcgg gtgtctcgcc gagcgcgacc gcgcggccgg gcgggaggcc
      721 ggagccgcgc tcggtccacg tcgtcccggc gtcgtgcgag acgaggagcg gcgggcggcg
      781 gtcgacgcag acgacgaccg ccgagccggc cgcgtcgacg tcgacgagga gcgacgagac
      841 ccaggcgggc gcgaggtcgg ccggccgtgg gcgctcgacc cgcccgccgg gctcgcgacc
      901 ggcgagcgcg tcgtctccgg ccgtcgcgcc gacctcgacg agccagagcc cgtcctcgca
      961 ctccgcgacc gcgatcacgc gggctccggg cgcaggcggc gctcgccgag gatcgcggcg
     1021 gccgcgagcg cgaacgcgag ggcgaagctc gggagcgacg agccgatctg cgtctccggc
     1081 ggccccgtcc gctcgacgag ctcgacccac caggacggcc cgagcgggtg aagccactgg
     1141 tagagcgcga agccggcgat ccaggcgccg accggcccgg cccggaacgc gggcgcggcg
     1201 aggaggtcgc ggccgtcgta cgacgcgccc gagaggagcc agtccgcgag caggacgccg
     1261 aagagcggca cgaagaacga gccgagcagg agcaggaacg tctcgtagct cgagagctcg
     1321 atcgtgagcg ccccgagcgt cgcgacggcc gccacgccgg cgaggaggac ccgctgcgag
     1381 gcgcgcggga ccaggttctg gagggacacg gcggtcgagt agacgttcgc gaacgcctcg
     1441 tccgcctcgt ccacggtgac ggcgaggagc gcgagcgcgc tcgcaagccc cgcggccgcg
     1501 acgccggcgg ggatctcggc cgggtccgag aggcccttgg cgagcacgag cagcccgccg
     1561 aggacgaaca tccacgtcgc ggcgaggaag tagccgccgc cggtgccgac gaacgcggcg
     1621 cggcgcgtcc ggccgaagcg cgtgtagtcg gccgcgagcg ggatccagct caccgtgatc
     1681 gcgatcacga gatccaccgc gccgacgaag gtcgtgccgc cctcgccggg cgcgtcccag
     1741 agcgcgcgga ggtcggcgcc gtcgagcgcc caccacgtga ggtagacgag cgaggcgagc
     1801 acgatccaga cggcgaactt ccggatcacc cgccggacga cgccgatcgg tccgagcagc
     1861 gcgagcgaga gcgccaccgc accggtcgcg acggtccacg cggcctcggc tcgcacgccg
     1921 aagagccgat ccgagagcgc gctcgccgcg gccgcgatga tcacgatctc gaaggtcgcc
     1981 cacccgaggc actgcgcgac gttcagcccg gtcgcgccgt acgacccgcg ccgcccgagc
     2041 ggtccgcgca tgagcaccat cgccggcacg cgggcgtccg cgccgatgag gcccgccgtg
     2101 ccgagcatga ggttcccgat caggctgccg accacgaggg cgagcagcgc ctcggggatc
     2161 gagagcgccg ggacgaggaa ggcggccgtc acgagcacga gcaggctcac gccgaggttc
     2221 ccccagagca gcgtcaggtc gagcgtgccg agcgtccgca gccgctcggg aacgggtgtg
     2281 acgccccagc ttccttcggt cgccaatcga gctcccttcg ccggaattac ccagatcagg
     2341 ttcgacgggt cgaggctcgc tccgtgcctc ctctcagccg ccgcgcgcgg ctccccggtc
     2401 gcgccggagc ttaccgggcg gtgtagaccg tgacctgcgt gagcgcgggc gcctcggtgc
     2461 cggcggcggg atcctggcgg agcacctggc cgacctcgtc cggcgtcggc gcgtcctcgt
     2521 cgacggtgag gacggtgaag ccggcccggc ggaggatctc gcgcgcctgc gcgccctccg
     2581 cgcccgtgac gtcgggaacc atgatcgcga cgagcgggtc ggggccgagc gagacgtcga
     2641 gccggaggcg ctcgtcggtg tcgagctcca cgcccgcctc cggctcctgg gcgacgacct
     2701 ggccggccgg cgcgtcgctc tcgaccgggt acgtgttcgc gacgagcgcg agatcctcgg
     2761 ccgcgcgccc ggcgtccgcg tggtcgaacc cgacgagctc gggcacgacg accgccgtcg
     2821 ccggcgcgac gaccacggtc tccgtcgtgg cgggcccggc ggcgtcgtcg tcgtcactcg
     2881 cgcggtcggc gaggacggcg acgaagatga tcgcggccgc gacgacggcc accgcgagga
     2941 gcgcgagcca gagccagggg ttgcggggcg gcggcggctc gtgggcgacc tcctccttga
     3001 cgacgtgctc ctcgatcacg cctggtggct acccgcttcg ccgcgcgtca agcgtgcgag
     3061 accctccacg ccgcgggcga gcccatcctc gtcgaagagg tacggcgtcg cgttgcgcag
     3121 ctcgtgcacg gggccgtcga gctcgtgcga cccgccggcg gcgttcagga ggtagcggag
     3181 cgggatctcg tggcagacga cgagcgttgt ctcccacggt gacgcgagca ggcgcgcgaa
     3241 gccctcggcg tagcgcgcgg ccgcgtcgtc gaggctctcc ccgccgggga acgggacgct
     3301 gcgcgcgttg ccgtgcttcc aggcccggta ctcctcgatc gtcttgccgt ccaggtcgcc
     3361 gacgcggatg tcgtcgagga gcggctcctc gtgcagcggg acgccgcggc cctcgagcgc
     3421 gagctcggcc gtctcgcggg tgcggaggaa ccgggtgacg acgcacgcct cgagcgggag
     3481 cccggcgagc tggacgccga gctggcgcga ctcctcgcgg cccttctccg tgagcccgac
     3541 cggaacggac gggtcgccgt tcacgcgccg ctcggcgttc agcgtggact cggcgtggcg
     3601 ggcgacgacg aagaggcgat ccatcagatg acgaatgcct cctcgcgggc cgttccacgc
     3661 gcgaagcgct cgagggcgaa gggcgagagg tcgagcgtcg gcacgcggcc ggcgacgagc
     3721 tcggcgagat gctcgccgac cgccgggctt tgctggaacc cgtggcccga gaagcccgtg
     3781 gcgtagagga accggccggg ctccgacgcc tcgccgacga tcgcgttgtg gtccggcgag
     3841 atttcgtaga agccggacca cgagctctgc accgggaggt cggcgagcgc gggcagacgc
     3901 cgcgtggccg ggccggccag ctcgtcgagc gtctgctcgc gcccgccgaa cgcgagtccg
     3961 ggcccctccg tgtggaagta gaagccggtc gagaagtcga tcgtgagcgg cagcggccgt
     4021 ggaaggccgc cggagtgcgg cgtgaagtgg atccagcggc gctcgccggt gacgggaagc
     4081 tcgaccccgg cgaggcgcgc gacctccggc gaccagacgc cggccgcgca gacgaccgcg
     4141 ccggtggcga ttcgcgcgcg ggtcgtctcg acggcggcga tgcgcccgcc ggcgacctcg
     4201 atgcgctcga ctgcctcgcc ctgccggatg cgaacgcccc gccgggcggc cgcctccgcg
     4261 tagccggcca cgaccgcctc ggcggtcgcg cgcccgtcga ccgggccgaa cgtcgctccc
     4321 acgaggccct cgggcgagag ctgcgggacg atcgagagcg cttcctcgac cgagagctcc
     4381 cgcgtcggca cgccgagccc gcgctggagc gcgaccgcgt cgcggaagcg ggcgacgtcc
     4441 tcctcgcggt cgagcaggaa gaggtagccg ctctgctcgt agtcgatcgg gcggccggtc
     4501 agcgcctcga agcgctcgaa ctcggcgatc gagcggagcg cgatccggac gttcagctcg
     4561 tccgcgaact gcgcgcggat cccgccggcc gacttcccgg tcgagccgga cgcgagcgtc
     4621 gcacgttcca cgagcacgac gtcggtcacc ccgagctccg tcaggtggaa ggcggtggag
     4681 gcgccgatcg cgccgccgcc gacgatcacg acctcggccg actcgtccgt cacggcgcga
     4741 tcgtacgcgg cggcccgtgg gataggctcg cgccgtgcat cggttcgagg tcgagagcgt
     4801 ccggcgcagg ctcgaggagg ggaacggcgg ctacgagatc gtccacgcct cgccggggct
     4861 cgaggtcggc gtctacgtcc tcgtcgcgcc cgagcccgac cgccagcagc cgcacgagga
     4921 cgacgaggtc tacgtcgggc tcgagggcca cggcgtgctc gaggtggcgg gcgagtcggt
     4981 ggcgatcggg ccgggggagg cgatcttcgt cgaggcgggc gtcgaccacc ggttcaccgc
     5041 gtacgagtcg ctctcggtgc tcgtcgtctt cacgcgcccg gcgtgaacgt cctcgtcacg
     5101 ggcgcgaccg ggttcctcgg acgccatctc tgcgcggcgc tcgtcgagcg cggggacgcg
     5161 gtgcgcgcgt tcgtgcggcc cgggactgac gcgggcgcgc tggaagcgct cggcgtcgag
     5221 gtcgtgcgcg gctcgctcgc ggacgccggc gcgctccggg cggcagcggc gggccgcgcg
     5281 ctcgtcttca acgcggtcgg gctcgtctcg tacgagtggc ggcagctcga ccggctgcgc
     5341 gaggcgaacg tcgagtcggt gcgccgcgtc ctcgccgccg tcgagccggg cgcgcggctc
     5401 gtgcacgtgt cgagcggcgc tgcggtgggc ccgtcgggct cccccgaccg gctggcggac
     5461 gaggcgcagc cgttccccga ggtcgcgcgc cggaacccgt actcggtgac gaagcacgac
     5521 gcggaggagc tcgtcttcgc ggcggtcgcc ggcgggctcg acgcggtcgt cgggaacccg
     5581 gcgatcgtgc tcgggcccgg cgacgtctac ggcagctcca cgtggccggt gcgccgctac
     5641 ctccagggga cgctccgctt tcacacgcgc ggcggcggcg cctcgttcgt ggacgtccgg
     5701 gacgccgcgg cgggcctcgt cgccctcgcg gagcggggcc gcacgggcga gcgctacatc
     5761 ctcacgtgcc gcgaggggaa cctcccgtgg aacgtgttct tccgccgcgt cggcgcggca
     5821 accggcgtgc ggcgcgtgat gctgccgctc ccgccgccgg tgctgcgtgc cgccgtgcgc
     5881 gtcgtcccgt ggccggtgaa gccgggcgag gcgcggacgg cgaccgagtg gtggatcttc
     5941 gaccccggca aggccgagcg ggagctcggg ttcacgaccc gcccgctcgc cgagacgatc
     6001 cgggacaccg cggccgacgg ccgctgagcc ctcgtccggc ctgcgggtcg ggacacggcc
     6061 ctacgggccc gtgcgggagg actcccagcg ggagacctcg aacgcgcggc cggggaagcc
     6121 gccgatcgcg agcaccgtcg tcccgggctc gacggcgacc gccttgcgca cgaccgggcg
     6181 ctcgcagaag acgagcgtgc cgccggccgc gtcgcgctcc tcgccgtcga gcgtgaaccg
     6241 tgcgacgccc gacacgacga cgtacagctc ctcgtgcccg tcctcgccct cgtcgtgctc
     6301 ctcgacgatc agctcgccgg cctcgcgcgc cacgtatgcg ttgcagccga acgccgtgat
     6361 cccgaagtgg tagcggacgg gccgccactc gccctccacg aggtcgctcg cgagcgcagg
     6421 gacgtcctcg atgcgggcga cgcggtaccg gggctcgggc tcgccggcca ccgctacgac
     6481 atggggatct ggcggaggac cttcggctcg atgcggtcga ggatctccgt caggctctcg
     6541 ccggtcaccg tgatgccgtg gttccggagc ccgatggccg cgtgcgcggg atcgggctcg
     6601 gcgtcgagga gctgggcgac cgaggtcgcg agctcctccg tcccgcacgg gtagttcacg
     6661 tccgtcgccg ggatgtcctg catccacgcg tgcacgtgga ggatcgcgcc gacgtccgga
     6721 tgcgcccggt agatcatcca gtgctcgatc gcgtcgaccg acacacggcg cggctcgacg
     6781 ttcggcggga cgctcatgat catcgtgttc cgctccggcg cgtagccgga gacgagcagg
     6841 atgtcccgcc ccgggatgtc gagcttcgtc ttgtccacgc cgctcgcgct catccagaag
     6901 cgcgtctcgt ccttgcgctg cgagaggttg ccgtacgaga ggccgccgat cccgtagagg
     6961 cgcttcacgt gccggagatc ctgctcgtcg agcagctcct cgatcgggaa cggggcgggc
     7021 aggaggtcga ggtcgcccat ccgcttgccc gcctcggcga tctcgtccgt cacctcgtcg
     7081 ccctcccaga gctcctcctc gagatcggtg cggaactcgt tgtcgatcac gagcttcgag
     7141 agcgcgagcg gggcgagccg ctcgacgacg ccctcggcga gctcggcgac gccgtccgtc
     7201 gcgtcgacgc cgtagtggcc gcgctccatc gtcgtgaacc agacgccctt ctccgggacg
     7261 aagcagagga cgatgttcgc gagcgcccgg acgagcagcg ggtagttcac cttgagaacg
     7321 tcgtcgggga cggcctgctg ctcgtggatc gcggccacga acgtcccgcg cgagcgccgc
     7381 cggaagggct tcggcgcggc cggatcaatg aagttcacga cgaggtccgc ctcgagggcg
     7441 cccgccccct cggcgcgctc gaagccgcgc tcctcgaggc acacgcccag ctcgcgggcg
     7501 aactcctcga acacgggcga ggtgggactg ccggcgaatg cgtagttcat ggcgggcatc
     7561 ctacgctgcc gcgaggcggc cgagcacgta cgggaggatc cc
//
LOCUS       DASTFC010000291        10402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1160552,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000291 DASTFC010000000
VERSION     DASTFC010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10402
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160552"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFO88_08600"
     CDS             complement(<1..488)
                     /locus_tag="VFO88_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HET9545624.1"
                     /translation="MRGFLLLEDGTRFDGRSVGAPGVAVGEAVFTTAMSGYQEVVTDP
                     SFAEQLVCFTAAMVGNYGVEEGRSESDRPHARGVLMRRAGGEAWTSWLAEHGIVALDE
                     VDTRSLVLHLREQGAMRAALVAGEASVPALLARIRAEPTMAGRALAGGVSTREPYVVG
                     RGS"
     gene            complement(485..1792)
                     /locus_tag="VFO88_08605"
     CDS             complement(485..1792)
                     /locus_tag="VFO88_08605"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9545625.1"
                     /translation="MTPLTAPNRPKDDLVVRGARVLDPTEGIDAVLDVRVDGGTIAAL
                     GAGLDANGHRVIDGAGLVLAPAFVDPHVHLRTPGREDEEDIASGTRAAAAGGYCAILA
                     MPNTDPVVDSASVLGSLRERARAEAEIPVGFLAAITKGQTGDELTEMVELAETGAAGF
                     SDDGQPVASPALLRRALQYGAAAGPTIALHCEERTLTRGGHMHEGETSAELGFGGYPS
                     IAESLMVARDLALARYEERPVHLLHLSARESVEELRRARAEGVAASGEVTPHHLTATD
                     ELVRSLDANVKMNPPLRAEPDRAALVDALRDGTIEAVATDHAPHARHEKEVPFEGAPF
                     GVTGLETAFSVLYTRLVQPGVLALETLLERMSAGPARVYGLEAPRIAQGAEANLVLLD
                     LAGTWCVQESGFRSKSANSWLLGDTLGGRVALTVAGGRVAFAA"
     gene            complement(1792..2790)
                     /locus_tag="VFO88_08610"
     CDS             complement(1792..2790)
                     /locus_tag="VFO88_08610"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET9545626.1"
                     /translation="MADVRPLVRDELLPPKPPERRHLISIGDLTRDDVERLLTLAKTF
                     AGTLDRDVKKLPTLRGRTVVNLFYESSTRTSSSFELAAKRLSADVMSIKASGSAVDKG
                     ESLKDTALTIGCYDPDVIVIRHPEIGAPNLVARFTEAHVVNAGDGKHQHPTQALLDLY
                     TIVEELGRVEGLHVAIVGDVLHSRVARSDVQALLLMGAHVTLVGPPPLIPRGIEAMGC
                     AVSHDIEAIKEADVVYVLRMQKERMLAGANYVPSLREYTARWGVTPERIRPGQKVMHP
                     GPMNRGVEIDGAVADMADALIERQVRSGLAVRMAVLYDLLTHGPVGVKVAEIQAVA"
     gene            complement(2794..3369)
                     /gene="pyrR"
                     /locus_tag="VFO88_08615"
     CDS             complement(2794..3369)
                     /gene="pyrR"
                     /locus_tag="VFO88_08615"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET9545627.1"
                     /translation="MAAPSQAEKILLDADALGRTIVRIAHEIVEGNRDLANVALVGIH
                     TRGVPLAQRLRRLIHEFTGTEVELGTLDITFHRDDLHVRGSEAPLHAQPVVRSTSLDF
                     PLEGMTCVLVDDVLYTGRTIRAAIEALFAYGRPGRVQLACLVDRGHRELPIRPDYVGK
                     NLPTSHGERIQVQLVELDEVDAVLLEPATER"
     gene            3492..4118
                     /locus_tag="VFO88_08620"
     CDS             3492..4118
                     /locus_tag="VFO88_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET9545628.1"
                     /translation="MIVHFTAFLGVVVLVIVTPGPDTALTIRNTLLGGRAAGLATAAG
                     VAAGQAVWALAAAVGIAALVQASQPAFTALRLLGAAYLVFLGLQSLAAAVRGHRRAAL
                     DVAARGAGLRPAVALRQGLLSNLANPKMAAFFTSLLPQFVVGPSFAPLFALGLVLCGL
                     TLVWLAAYAVAVARAGDLLRRPLVRRAIDAVTGAVLVALGLRVAADRR"
     gene            complement(4266..5015)
                     /locus_tag="VFO88_08625"
     CDS             complement(4266..5015)
                     /locus_tag="VFO88_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9545629.1"
                     /translation="MADPGGDAAFAELAARYDRLRPVDDGWWEVFDALVEAGRLETAG
                     RVLDIGCGTGRLVEVLARRGPRVWGVDPSEEMLAQARGCRPPGGGFKQAHAERLPFKD
                     GWFDGAVMRQVVQHLELDAAFTEAARVLRPGGRLAIATFHPHHFDTVWIARLIPRVSE
                     IDRERFPSPERLTEALRAAGFAEPQAKRIRQATTLTRAEALERLRGRFISTLHLLRED
                     EVAEAIARAERELPETVESTLDWLILGAERA"
     gene            complement(5024..5152)
                     /locus_tag="VFO88_08630"
     CDS             complement(5024..5152)
                     /locus_tag="VFO88_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545630.1"
                     /translation="MEQHPTALDDAPTQVLVDERSLVAAQEAIEAMSEPDDLLGGR"
     gene            complement(5124..6224)
                     /gene="rnz"
                     /locus_tag="VFO88_08635"
     CDS             complement(5124..6224)
                     /gene="rnz"
                     /locus_tag="VFO88_08635"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET9545631.1"
                     /translation="MDLDLVFLGTAGSVPTARRAPSALLLRRGGERLLFDCGEGTQRQ
                     MLRSSVGLVDLDEVFLTHFHADHTLGLPGMMKTFALRGRDATLTVYGPPGLEELFGSL
                     RAIFGRLTYPYRLVELRPGDELARDGYVLRAVRVDHRSSAIGYALVEDQRPGRFDVEA
                     ATALGVPPGPLFGALQRGEDVTVPGGVTVRPEQVLGAARSGRRIVLTGDTRPAAGVLE
                     AAQGADVLVHDATFGDEEAERAEETAHATAREAAELARAAGVRMLVLTHLSNRYFGPE
                     IAREARAVFPETVVPKDFDIIDVPLPERGGPELVKGGALARREEAGTVGEGSGGGGGG
                     PRGGGGDPDDPPVGGDRVGAPSGGGAAPDGSR"
     gene            complement(6256..6510)
                     /locus_tag="VFO88_08640"
     CDS             complement(6256..6510)
                     /locus_tag="VFO88_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9545632.1"
                     /translation="MFVPKPVALLVVSLLVVFVALYAARPTRSAGPEARYVVQPGDTL
                     WTIASERYDGDPRQAIWRIKEHNGLESSALAPGLVLRLPG"
     gene            complement(6548..7018)
                     /locus_tag="VFO88_08645"
     CDS             complement(6548..7018)
                     /locus_tag="VFO88_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9545633.1"
                     /translation="MIALLGDTHMPRGSRALPDGCLRVLGAADLILHTGDLTGDSVLA
                     LLEAYAPVRAVQGNMDEWPVRERLPERVTVEAEGLRIGLVHDAGPAAGRHARLRAWFP
                     ACEIVAYGHTHQPEIVRDGGVWIVNPGSPTERRRAPGHTMAVVRDGIPQLVALD"
     gene            complement(7031..8143)
                     /locus_tag="VFO88_08650"
     CDS             complement(7031..8143)
                     /locus_tag="VFO88_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET9545634.1"
                     /translation="MPERAVERLAEVAVSYSTRVRPGDLVVIESGPLAAPLVREIYRR
                     VLEAGGHPLVHVALEETQEELLREGSDAQLEFVSPARREEIEAADVRIVLDAEENTRT
                     LFAADPRRQALHKRARKPLGDRLFERSAAGELRWLVTLYPTHAAAQDAGMSLADYAEF
                     VYRAGLLDEDDPVGAWRALGERLDRVAEWLGTKDELHVLADGTDLRLQVGGRRWVASR
                     GFENFPDGEVFTGPVETSVDGTVRSSFPAAYQGRIVEEVELRFEGGEVVAVEAARGRD
                     FLEEMLALDEGARRMGEFAFGMNEAITRFTLNTLFDEKIGGTMHFALGKSYPETGGVN
                     NSALHWDLVCDLRRGSEVYADGELVYRNGRFLDGLF"
     gene            complement(8136..9272)
                     /locus_tag="VFO88_08655"
     CDS             complement(8136..9272)
                     /locus_tag="VFO88_08655"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET9545635.1"
                     /translation="MIDPRVERLAELVVHYSLEIGEGQVVRIDGFDVAAPLALAIYRE
                     ALRAGAHPYTNLTLDGLHELLVKHGSDDQLAYVSPTQWREIEALDAHVTIWSEVNTRS
                     FGALDERRHGALIAAQRALSNRRWERIAAGELRWCGTLHPTNGHAQDADMSLRDYEAF
                     YFAACHADTPDPAAYWRSLSATLNARAQSLASVRELRILGPDTDLRVGVGGRTWIAAD
                     GKYNLPDGEVFTSPVETETEGEIRFTFPAIFSGREVEDVRLRFEGGRVVAAEASRGEA
                     YLRELLDLDEGARVLGEVAFGLNYEIDRFTRNILLDEKIGGTMHLALGAGFAQAGGAN
                     TSALHWDLICDLRREGEILADGERVWKDGSFVAPLDVAVAGERA"
     gene            complement(9292..10326)
                     /locus_tag="VFO88_08660"
     CDS             complement(9292..10326)
                     /locus_tag="VFO88_08660"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HET9545636.1"
                     /translation="MQKRFLILAEGYSHDPHYGKTMRGIVRYRRDEVVAILDSKRAGE
                     EYDAVPIVGSVKDALAYEPDTALVGVAVAGGRLGPEWRALLRDAISAGLDVEAGMHEF
                     LADDPELSLLAHERGVELRDTRRPPDGLSVPTGANLELDAAVVLTVGSDCAVGKKTMA
                     LELDREARSRGIRSVFVPTGQTGIMIAGWGIAVDAVVADFLAGAAERLVVEGARRGDL
                     LWVEGQGALLHPQFSGVTLGLYHGSAPHALVLCHVAGQTELEGCPGHALPPLPELVRL
                     YEAVSLPARPATVAAVALNTSLLDEASARAAIAEAERETGLVADDPVRFGAGRLVDAV
                     LARLPGQAPR"
BASE COUNT         1342 a         3879 c         3813 g         1368 t
ORIGIN      
        1 gacccgcggc cgacgacgta cggctcccgc gtcgacaccc cgccggcgag cgcgcgcccg
       61 gccatcgtcg gctccgcgcg gatccgggcg agaagcgcgg ggacggaggc ctcgccggcg
      121 acgagcgccg cgcgcatcgc gccctgctcc cgcaggtgga ggacgagcga gcgcgtgtcc
      181 acctcgtcga gcgccacgat cccgtgctcg gcgagccagc tcgtccacgc ctcgccgccc
      241 gcgcggcgca tgaggacgcc gcgcgcgtgc gggcggtcgc tctctgagcg gccttcctcc
      301 acgccgtagt tgccgaccat cgcggcggtg aagcagacga gctgctccgc gaagctcggg
      361 tctgtgacga cctcctggta gcccgacatc gccgtcgtga agacggcctc gccgaccgcc
      421 acgccgggcg cgccgaccga gcggccgtcg aaccgcgtcc cgtcctcgag gagcaggaag
      481 cccctcacgc ggcgaacgcc acccggccgc cggcgacggt gagcgcgacc cgcccgccga
      541 gcgtgtcgcc gaggagccac gagttggccg acttcgagcg gaagccggac tcctgcacgc
      601 accacgtccc ggcgaggtcg agaaggacga ggttcgcctc cgcaccctgc gcgatccgcg
      661 gcgcctcgag gccgtacacc cgcgcgggac cggcgctcat ccgctcgagc agcgtctcga
      721 gcgccaggac gccgggctgc acgagccgcg tgtagaggac ggagaacgcg gtctcgaggc
      781 cggtgacgcc gaacggggcg ccctcgaacg gcacctcctt ctcgtgccgc gcgtgcggcg
      841 cgtggtcggt cgcgaccgcc tcgatcgtcc cgtcgcggag cgcgtcgacg agcgcggcgc
      901 ggtccggctc ggcacggagc ggcgggttca tcttcacgtt cgcgtcgagc gagcggacga
      961 gctcgtccgt cgcggtcagg tggtgcggcg tgacctcgcc gctcgcggcc acgccctccg
     1021 cgcgggcgcg gcgtagctcc tcgaccgact cgcgcgcgga gagatggagc aggtgcacgg
     1081 gccgctcctc gtagcgggcg agcgcgaggt cgcgcgcgac catgaggctc tcggcgatcg
     1141 acgggtagcc gccgaagccg agctccgccg acgtctcgcc ctcgtgcatg tggccgccgc
     1201 gggtgagcgt ccgctcctcg cagtggagcg cgatcgtcgg gccggccgcc gcgccgtact
     1261 ggagcgcgcg gcgcaggagc gcgggcgagg cgaccggctg cccgtcgtcc gagaacccgg
     1321 ccgcgcccgt ctcggcaagc tcgaccatct ccgtcagctc gtcgcccgtc tgccccttcg
     1381 tgatcgcggc gaggaacccg accgggatct ccgcctcggc gcgcgcccgc tcgcggagcg
     1441 acccgagcac ggacgccgag tcgacgaccg ggtctgtgtt cggcatggcg aggatcgcgc
     1501 agtagccgcc ggcggcggcc gcccgggtcc ccgaggcgat gtcctcctcg tcctcccggc
     1561 cgggcgtgcg caggtggacg tgcgggtcga cgaaggcggg cgcgagcacg agcccggcgc
     1621 cgtcgatcac gcgatggccg ttcgcgtcga gcccggcgcc gagcgcggcg atcgtgccgc
     1681 cgtcgacgcg gacgtcgagg acggcgtcga tcccctcggt cgggtcgagg acgcgcgcgc
     1741 cgcggacgac gagatcgtcc ttcggcctgt tcggggcggt gagcggcgtc atcacgcgac
     1801 cgcctggatc tcggccacct tcacgccgac cgggccgtgc gtgaggaggt cgtagaggac
     1861 tgccatccgg accgcgagcc cgctccgcac ctggcgctcg atgagcgcgt cggccatgtc
     1921 cgcgacggcc ccgtcgatct cgacgccgcg gttcatcggg cccggatgca tgaccttctg
     1981 ccccgggcgg atccgctccg gcgtcacgcc ccagcgcgcc gtgtactcgc gcaggctcgg
     2041 gacgtagttg gcgcccgcga gcatccgctc cttctgcatc cggagcacgt acacgacgtc
     2101 cgcctccttg atcgcctcga tgtcgtggga gaccgcgcag cccatcgcct cgatcccccg
     2161 cgggatgagc ggcgggggac cgacgagcgt cacgtgcgcg cccatgagga ggagcgcctg
     2221 gacgtccgag cgcgcgaccc gcgagtgcag gacgtcgccg acgatcgcga cgtggaggcc
     2281 ctcgacccgc ccgagctcct cgacgatcgt gtagaggtcg aggagcgcct gcgtcgggtg
     2341 ctggtgcttg ccatcgccgg cgttcacgac gtgcgcctcc gtgaagcgcg cgacgaggtt
     2401 cggcgcgccg atctccggat ggcggatgac gatgacgtcg gggtcgtagc agccgatcgt
     2461 cagcgccgtg tccttgaggg actcgccctt gtcgacggcg gagccgctcg ccttgatcga
     2521 catgacgtcg gcggacaggc gcttcgcggc gagctcgaag ctcgaggacg ttcgcgtcga
     2581 gctctcgtag aagaggttca cgaccgtgcg gccgcggagc gtcgggagct tcttcacgtc
     2641 gcggtcgagc gtcccggcga aggtcttcgc gagcgtgagc agccgttcga cgtcgtcgcg
     2701 cgtgaggtcg ccgatcgaga tcaggtgccg ccgctcgggc ggcttcggag gcaggagctc
     2761 gtcgcggacg agcggtcgca cgtcagccat tcgtcacctt tcggtcgcgg gttcgaggag
     2821 cacggcgtcg acctcgtcga gctcgacgag ctgcacctgg atgcgctcgc cgtgcgaggt
     2881 cggcaggttc ttgccgacgt agtccggccg gatcgggagc tcgcggtgcc cgcgatcgac
     2941 gaggcacgcg agctggacgc gcccggggcg cccgtacgcg aagagcgcct cgatcgcggc
     3001 gcggatcgtc cggccggtgt agaggacgtc gtcgacgagc acgcacgtca tcccctcgag
     3061 cgggaagtcg aggctcgtcg agcggacgac cggctgcgcg tgaagcggcg cttcgctacc
     3121 gcgcacgtgg aggtcgtccc ggtggaacgt gatgtcgagc gtcccgagct cgacctcggt
     3181 gcccgtgaac tcgtggatca gccggcggag gcgctgcgcg agcgggacgc cgcgcgtgtg
     3241 gatgccgacg agcgcgacgt tcgcgaggtc ccggttcccc tcgacgatct cgtgtgcgat
     3301 gcggacgatc gtgcggccga gcgcgtcggc atcgagcagg atcttctccg cctgggaggg
     3361 cgcagccact ctcgcaactc ctttccggcc tcacgggacc gagttaaagg gtcgggcgga
     3421 gtatagggtc gcccgtcgga cggccctcgc cgagccttgc ggggagcgct tcggcgcgcg
     3481 acgatggcgc cgtgatcgtc cacttcaccg cgttcctcgg cgtcgtcgtc ctcgtgatcg
     3541 tcacgccggg gccggacacg gcgctcacga tccggaacac gctgctcggc gggcgcgcgg
     3601 cggggctcgc gacggcggcc ggggtcgcgg cgggacaggc cgtctgggcg ctcgccgccg
     3661 cggtcgggat cgccgcgctc gtgcaggcgt cgcagccggc cttcaccgcg ctgcgcctcc
     3721 tcggcgcggc ctacctcgtc ttcctcggcc tgcagtcgct cgccgccgcg gtgcgcgggc
     3781 accggcgtgc ggcgctggac gtggcggcgc gcggtgcggg gctgcgaccc gccgtcgcgc
     3841 tccgccaggg gctcctgagc aacctcgcga acccgaagat ggccgcgttc ttcacgagcc
     3901 tcctccccca gttcgtcgtc ggtccgtcgt tcgcgccgct cttcgcgctc gggctcgtgc
     3961 tctgcgggct gacgctcgtc tggctcgccg cctacgcggt cgcggtggct cgggcaggcg
     4021 acctcctgcg ccggccgctc gtccggcggg cgatcgacgc ggtgaccggc gccgtgctcg
     4081 tcgcgctcgg cctgcgggtc gccgccgacc gccgctgacg ggcgccggcc ctgcggcggc
     4141 cgtggccgct ccggccattg tcccgtgcca ggcaccggcc gtggccgcgg gggacaacgg
     4201 ccgctcgggc cactgtcccg tgccaggcac cggccgtggc cgccggggac gtggccggac
     4261 ggacgctacg cgcgctcggc gccgagaatc agccagtcga gcgtcgactc gaccgtctcg
     4321 gggagctcgc gctcggcgcg ggcgatcgcc tcggcgacct cgtcctcgcg gagcaggtgg
     4381 agcgtcgaga tgaagcggcc gcggaggcgc tcgagcgcct ccgcccgcgt gagcgtcgtc
     4441 gcctggcgaa tccgcttcgc ctgcggctcg gcgaacccgg cggcgcggag ggcctcggtc
     4501 agccgctcgg gggacggaaa ccgctcgcgg tcgatctcgg acacgcgcgg gatcaggcgg
     4561 gcgatccaca cggtgtcgaa gtggtgggga tggaacgtcg cgatcgcgag ccggccgccg
     4621 ggccgaagga cgcgcgcagc ctcggtgaac gcggcgtcga gctcgagatg ctggacgacc
     4681 tggcgcatga ccgcgccgtc gaaccatccg tccttgaacg gcagccgctc cgcgtgcgcc
     4741 tgcttgaagc cgccgccggg cggacggcag ccgcgcgcct gcgcgagcat ctcctccgac
     4801 gggtcgacgc cccacacccg cgggccgcgg cgggcgagca cctcgacgag gcggcccgtc
     4861 ccgcagccga tgtcgaggac gcgcccagcc gtttcgagcc gcccggcctc gacgagcgcg
     4921 tcgaagacct cccaccatcc gtcgtcgacc gggcgcagcc ggtcgtagcg cgcggccagc
     4981 tccgcgaacg cggcgtcgcc cccggggtcg gccacgagcg ctgttagcgg ccgccgagga
     5041 ggtcgtcggg ctcgctcatt gcctcgatcg cctcctgcgc cgcgacgagc gaccgttcgt
     5101 cgacgagcac ctgcgtcggc gcgtcatcga gagccgtcgg gtgctgctcc accgccggat
     5161 ggagctccga ctcgatcccc gccgaccgga ggatcgtctg gatctcctcc gcctccgcga
     5221 ggtccgccgc caccgccacc tgaaccttct ccaacggtgc ccgcctcctc cctccgtgcg
     5281 agtgcgccgc ccttcacgag ctccgggccg ccccgctcgg ggagcgggac gtcgatgata
     5341 tcgaagtcct tcggcacgac cgtctcgggg aagacggccc gcgcctcgcg cgcgatctcg
     5401 ggcccgaaat agcggttcga gaggtgcgtg aggacgagca tccggacgcc cgccgcgcgc
     5461 gcgagctcgg ccgcctcgcg ggcggtcgcg tgcgcggtct cctcggcccg ctcggcctcc
     5521 tcgtcgccga acgtcgcgtc atggacgagg acgtcggcgc cctgcgcggc ctcgagcacg
     5581 ccggccgccg gccgcgtgtc gccggtcagg acgatccgac ggcccgaccg cgccgcgccg
     5641 agcacctgct ccggccgcac ggtcacgccg ccgggcacgg tcacgtcctc gccgcgctgg
     5701 agcgcgccga agagcggccc cggaggcacg ccgagcgccg tcgccgcctc gacgtcgaac
     5761 cggccgggcc gctgatcttc cacgagcgcg tagccgatcg cgctcgaccg gtggtcgacg
     5821 cgcacggcgc gcagcacgta gccgtcgcgc gcgagctcgt cgcccggccg cagctcgacg
     5881 agccggtacg ggtacgtgag ccgcccgaag atcgcgcgaa gcgacccgaa gagctcctcg
     5941 agcccgggcg gcccgtagac ggtcagggtc gcgtcgcgcc cccgcagggc gaaggtcttc
     6001 atcatcccgg gcaggccgag cgtgtggtcg gcgtggaagt gggtcaggaa gacctcgtcg
     6061 aggtcgacga gcccgaccga cgagcggagc atctgccgct gcgtcccctc gccgcagtcg
     6121 aagagcagcc gctcgccgcc ccggcggagg agaagcgcag agggcgcgcg ccgcgcggtc
     6181 ggcaccgagc cggccgtgcc gaggaacacg agatcgagat ccacggggag agtctagggg
     6241 cgcctcctct ccgcctcagc ccggcagcct gagcacgagc cccggcgcga gcgccgacga
     6301 ctcgagcccg ttgtgctcct tgatccgcca gatcgcctgc ctgggatcgc cgtcgtagcg
     6361 ctccgacgcg atcgtccaga gcgtgtcgcc cggctgcacg acgtagcgcg cctcgggccc
     6421 cgcgctgcgc gtcggccgcg cggcgtagag cgcgacgaac accacgagga gcgacacgac
     6481 gagcagtgcg accggcttcg gtacgaacat atgttcgcat ggtagcggac cagcccggac
     6541 ggcgtgctca gtcgagcgcg acgagctggg gtatgccgtc ccggacgacc gccatcgtgt
     6601 gcccgggcgc tcggcggcgc tcggtcgggc tgcccgggtt cacgatccag acgcccccgt
     6661 cgcgcacgat ctccggctgg tgcgtgtggc cgtaggcgac gatctcgcac gccggaaacc
     6721 acgcacgaag ccgcgcgtgg cggccggcgg ccggcccggc gtcgtggacg aggccgatcc
     6781 ggagcccctc cgcctcaacg gtgacccgct cgggcaggcg ctcgcggacc ggccactcgt
     6841 ccatgttccc ctgcacggcg cgcaccggcg cgtacgcctc gagcagcgcg agcaccgagt
     6901 cgcccgtgag gtcgccggtg tgcaggatga ggtcggcggc ccctagcacg cgcaggcacc
     6961 cgtccggcag ggcgcgcgac ccgcgcggca tgtgggtgtc gccgaggagc gcgatcacgg
     7021 cgccgcgccg ctagaacagg ccgtcgagga agcgcccgtt gcggtagacg agctcgccgt
     7081 ccgcgtagac ctcgctcccc cggcgcaggt cgcagacgag gtcccagtgg agcgccgagt
     7141 tgttcacgcc gccggtctcc ggatacgact tgccgagggc gaagtgcatc gtgccgccga
     7201 tcttctcgtc gaagagcgtg ttcagcgtga accgcgtgat cgcctcgttc atcccgaacg
     7261 cgaactcgcc catccgccga gcgccctcgt cgagcgcgag catctcctcg aggaagtcgc
     7321 gtccgcgggc ggcctcgacg gccaccacct cgccgccctc gaaccggagc tcgacctcct
     7381 cgacgatccg gccctggtag gcggccggaa acgacgagcg aacggtgccg tcgacgctcg
     7441 tctcgaccgg accggtgaac acctcgccgt ccgggaagtt ctcgaagccg cggctcgcca
     7501 cccaccggcg gccgccgacc tggaggcgca ggtcggtccc gtccgcgagg acgtgcagct
     7561 cgtccttcgt ccccagccac tccgcgacgc ggtcgagccg ctctccgagc gcccgccagg
     7621 cgccgacggg gtcgtcctcg tcgagcagcc cggcgcggta gacgaactcg gcgtagtcgg
     7681 cgagcgacat gcccgcgtcc tgggcggccg cgtgcgtcgg atagagggtc acgagccagc
     7741 gcagctcgcc cgccgccgag cgctcgaaga gccggtcgcc gagcggcttg cgcgcccgct
     7801 tgtggagagc ctggcggcgc gggtcggcgg cgaagagcgt gcgcgtgttc tcctccgcgt
     7861 cgagcacgat cctgacgtcg gccgcctcga tctcctcgcg ccgggccggg ctcacgaact
     7921 cgagctgggc atccgagccc tcgcggagga gctcctcctg cgtctcctcg agcgcgacgt
     7981 ggacgagcgg gtgcccgccc gcctcgagga ctcgccggta gatctcccgc acgagcgggg
     8041 cggcgagcgg gcccgactcg atcacgacga gatcgcccgg ccggacgcgg gtcgagtagg
     8101 agacggcgac ctcggcgagg cgctcgacgg cgcgctcagg cacgctcgcc cgccacggcg
     8161 acgtcgagcg gcgcgacgaa cgagccgtcc ttccagaccc gctcgccgtc cgcgaggatc
     8221 tcgccttcgc ggcggaggtc gcagatgaga tcccagtgga gcgccgacgt gttcgccccg
     8281 ccggcctgcg cgaagcccgc gccgagcgcg aggtgcatcg tcccgccgat cttctcgtcc
     8341 agcaggatgt tgcgcgtgaa gcggtcgatc tcgtagttca ggccgaacgc gacctcgccc
     8401 aggacgcgcg cgccctcgtc gaggtcgaga agctcccgga gataggcctc gccgcgcgac
     8461 gcctccgccg cgacgacccg gccgccctcg aagcgcagcc ggacgtcctc gacctcgcgc
     8521 ccgctgaaga tcgccgggaa cgtgaagcgg atctcgccct cggtctcggt ctcgacgggg
     8581 ctcgtgaaga cctcgccgtc cggcaggttg tacttcccgt cggcggcgat ccaggtgcgg
     8641 ccgccgacgc cgacgcggag gtcggtgtcc ggcccgagga tccgcagctc gcgcacggag
     8701 gcgagcgact gcgcccgcgc gttgagcgtc gccgagaggg agcgccagta cgcggccggg
     8761 tcgggcgtgt ccgcgtgaca ggcggcgaag tagaacgcct cgtagtcccg gagcgacatg
     8821 tccgcgtcct gcgcgtgccc gttcgtcgga tggagcgtcc cgcaccagcg gagctcgccc
     8881 gccgcgatcc gctcccagcg ccggttcgag agcgcgcgct gcgcggcgat gagcgcgccg
     8941 tggcgccgct cgtcgagcgc cccgaacgac cgcgtgttga cctccgacca gatcgtgacg
     9001 tgcgcgtcga gcgcctcgat ctcgcgccac tgggtcggcg acacgtacgc gagctggtcg
     9061 tccgagccgt gcttcacgag gagctcgtgg aggccgtcga gcgtcaggtt cgtgtacggg
     9121 tgcgccccgg cccgtagcgc ttcccggtag atcgcgagcg cgagcggcgc agcgacgtcg
     9181 aagccgtcga tccgcacgac ctgcccctcg ccgatctcga gcgagtagtg gacgacgagc
     9241 tccgcgaggc gctccacgcg cgggtcgatc atcagcgtct tataccagga gtcaacgagg
     9301 cgcttgaccc ggcagccggg cgaggacggc gtccacgagc cggcccgccc cgaagcggac
     9361 cgggtcgtcg gcgacgagcc cggtttcccg ctcggcctcc gcgatcgccg cgcgcgcgct
     9421 cgcctcgtcg agcaggctcg tgttcagcgc gacggcggcc acggtcgccg gcctggccgg
     9481 aagggacacg gcctcgtaga gccgcacgag ctccgggagc ggcggcaggg cgtgccccgg
     9541 gcagccctcg agctccgtct ggccggcgac gtggcagagc acgagcgcgt gcggcgcgct
     9601 cccgtggtag agcccgagcg tgacgcccga gaactgcgga tggaggaggg caccctggcc
     9661 ctcgacccac aggaggtcgc cgcggcgcgc gccctcgacg acgagccgct cggccgcgcc
     9721 cgcgaggaag tccgcgacga ccgcgtcgac cgcgatcccc cagccggcga tcatgatccc
     9781 cgtctggccg gtcgggacga acaccgagcg gatcccccgg ctgcgcgcct cgcggtcgag
     9841 ctcgagcgcc atcgtcttct tgccgaccgc gcagtccgag ccgaccgtga ggacgacggc
     9901 cgcgtcgagc tcgagattgg ctccggtcgg gacgctcagc ccgtccggcg gccggcgggt
     9961 gtcgcggagc tcgacgccgc gctcgtgcgc gaggagcgag agctccgggt cgtcggcgag
    10021 gaactcgtgc atgcccgcct cgacgtcgag cccggccgag atcgcgtcgc gcaggagcgc
    10081 acgccactcg gggccgagcc gcccgccggc gacggcaacc ccgacgagcg ccgtgtccgg
    10141 ctcgtaggcg agcgcgtcct tgacggagcc gacgatcggg accgcgtcgt actcctcccc
    10201 ggcgcgcttc gagtcgagga tcgccacgac ctcgtcccgg cggtaccgga cgatgccgcg
    10261 catcgtcttc ccgtagtgcg ggtcgtgcga gtagccctca gcgaggatca ggaagcgctt
    10321 ctgcacgggc tcccagcccc ggctcgtcgg agggcgcctg caccccgtcc tcgcgaaatt
    10381 cgacccccgg ccacgggtcc tc
//
LOCUS       DASTFC010000292         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_89741,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000292 DASTFC010000000
VERSION     DASTFC010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89741"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFO88_08665"
     CDS             <1..700
                     /locus_tag="VFO88_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9545637.1"
                     /translation="DRLSLRAAEEATAAGNPLDVGAALIVELDGPTAECEARLENVLG
                     VCDRNGARGVRVAETAAERELVWRARKAAFAAMGRISPSYYVQDSVIPRTRLAEVLAR
                     IDELSASYGLAVANVFHAGDGNLHPLVAYDARVPGQAERAEELAGEIVLACVAAGGSI
                     TGEHGVGVDKKRFMPAMFSEDDLAAFQKLRCAFDPAQRANPGKVMPTPRLCGEVPGPY
                     REHPLEAAGIAERL"
     gene            697..1770
                     /locus_tag="VFO88_08670"
     CDS             697..1770
                     /locus_tag="VFO88_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9545638.1"
                     /translation="MSARAETPGSAGEAAGLLASDARVRPRGGGTKWGWADVPEPDVV
                     LSTAGLAGIRELNAGDLTAVVGAGTPLAEVQAACAEHGLMLALDPPGDGATVGGVVAA
                     ADSGPLRHRYGAARDLLLGITAALPDGTVARSGGKVIKNVAGYDLPKLFAGSLGALGT
                     IAEVVVRLHPRPATTATAVGAGGAAALARATLALAAAPLELERLDVRWAAREGALLAQ
                     AGGVSAAARAERAAGLMREAGLEAAAQEDDDEVWAEQRAGQRDPAGTVLRVAGLPAGL
                     ERVLAAAERHGAALVGRAGVGTSWLRLDPAAVDAVRGELAPFPCAVTDGPPGLDRLGP
                     RDPALVALERRVRERFDPAGALA"
     gene            1767..>2065
                     /locus_tag="VFO88_08675"
     CDS             1767..>2065
                     /locus_tag="VFO88_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9545639.1"
                     /translation="MTAFDAHRPPAAEDLDTCVHCGFCLPTCPTYVLWGEEMDSPRGR
                     IVLMDAALRDADVLSDAMVTHWDRCLGCMACVTACPSGVRYDRLLEDTRPQVERN"
BASE COUNT          223 a          820 c          785 g          237 t
ORIGIN      
        1 ggaccgcctc tcgctccgcg ccgccgagga ggcgaccgcg gcgggaaacc cgctcgacgt
       61 cggcgccgcg ctgatcgtcg agctcgacgg gccgaccgcc gagtgcgagg cgcgcctcga
      121 gaacgtgctc ggcgtctgcg accgcaacgg cgcccgcggc gtccgcgtgg ccgagaccgc
      181 ggccgagcgg gagctcgtct ggcgcgcccg caaggccgcg ttcgccgcca tgggccggat
      241 ctcgccctcg tactacgtcc aggactcggt catcccgcgc acgcgcctcg ccgaggtgct
      301 cgcgcggatc gacgagctgt ccgcgtccta cggcctcgcc gtcgcgaacg tcttccacgc
      361 gggggacggg aacctccacc cgctcgtcgc atacgacgcg cgcgtccccg gacaggccga
      421 gcgggccgag gagctcgcgg gggagatcgt cctcgcgtgc gtcgcggcgg gcggctcgat
      481 caccggcgag cacggcgtcg gcgtggacaa gaagcgcttc atgccggcga tgttctccga
      541 ggacgacctg gcggcgttcc agaagctccg ctgcgcgttc gacccggcgc agcgggcgaa
      601 cccggggaag gtcatgccga cgccgcgcct ctgcggcgag gtgcccgggc cgtaccgcga
      661 gcaccccctc gaggcggccg ggatcgcgga gcgcctgtga gcgcgcgcgc cgagactccc
      721 ggatcggccg gggaggcggc cgggctgctc gcgtccgacg cccgcgtgcg cccgcgaggc
      781 ggcgggacga agtggggctg ggccgacgtg cccgagcccg acgtcgtcct ctcgaccgcc
      841 gggctcgccg ggatccggga gctgaacgcg ggcgatctca ccgccgtcgt cggcgcgggc
      901 acgccgctcg ccgaggttca ggcggcgtgc gcggaacacg ggctcatgct cgccctcgac
      961 ccgcccgggg acggcgcgac cgtcggcggg gtcgtcgccg cggccgactc cggcccgctg
     1021 cgccaccgct acggcgcggc gcgcgacctc ctgctcggga tcacggcggc gctccccgac
     1081 gggacggtcg cccgctccgg cgggaaggtg atcaagaacg tcgcgggcta cgacctcccc
     1141 aagctcttcg ccggctcgct cggcgcgctc ggaacgatcg ccgaggtcgt cgtccggctc
     1201 cacccgcgcc cggccacgac ggcgaccgcg gtcggagccg gaggcgcggc ggcgctcgcc
     1261 cgcgccacgc tcgcgctcgc cgccgccccg ctcgagctcg agcgtctcga cgtccgctgg
     1321 gcggcccggg agggcgcgct cctcgcgcag gccggcggcg tctcggcggc cgcgcgggcg
     1381 gagcgggcgg ccggcctcat gcgcgaagcg ggcctggagg cggcggcgca ggaggacgac
     1441 gacgaggtct gggcggagca gcgcgcgggc cagcgcgacc cggcgggaac cgtcctccgc
     1501 gtcgccggcc tccccgccgg gctcgagcgc gtgctcgccg cggccgagcg ccacggcgcc
     1561 gcgctcgtcg ggcgcgccgg ggtcggcacc tcgtggctcc ggctcgatcc cgccgcggtc
     1621 gacgccgtcc gcggggagct cgccccgttc ccctgcgccg tgaccgacgg cccgcccggc
     1681 ctcgaccggc tcggcccgcg cgatcccgcg ctcgtcgcgc tcgagcggcg cgtccgcgag
     1741 cgcttcgacc ccgccggagc cctcgcgtga ccgcgttcga cgcccaccgg ccgccggccg
     1801 cagaggatct ggacacgtgc gtccactgcg gcttctgcct cccgacgtgc ccgacctacg
     1861 tcctctgggg cgaggagatg gactcgccgc gcgggcgcat cgtgctcatg gacgccgcgc
     1921 ttcgcgacgc cgacgtcctc tcggacgcga tggtcacgca ctgggaccgc tgcctcggct
     1981 gcatggcgtg cgtgaccgcg tgcccgtccg gcgtgcgcta cgaccggctg ctcgaggaca
     2041 cccgtccgca ggtcgagcgc aacca
//
LOCUS       DASTFC010000293         5913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1243417,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000293 DASTFC010000000
VERSION     DASTFC010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243417"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFO88_08680"
     CDS             complement(<1..243)
                     /locus_tag="VFO88_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545640.1"
                     /translation="MRIVVVGAVLAALALLPVAAGCGGESELEEAQAVLNDARDAYAA
                     EEESGRDFSDGPCIADPLPDREDWVVVVVREPRDEDR"
     gene            complement(243..755)
                     /locus_tag="VFO88_08685"
     CDS             complement(243..755)
                     /locus_tag="VFO88_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9545641.1"
                     /translation="MSERPPFHVRKAEPGDAAELVELALAVGSEPEGWLVTNGTWRSV
                     SDERRYVRAIRRSKAAAVLVAEAPEGIVGRLSLSRDPHPASRHVADLGLMVAADWRRR
                     GVGRALMLGAEEWARGAGVRKLELHVLPNNDAAIALYESLGYEREGVRRNHYRRGADL
                     VDAVLMAKEV"
     gene            complement(752..2314)
                     /gene="ggt"
                     /locus_tag="VFO88_08690"
     CDS             complement(752..2314)
                     /gene="ggt"
                     /locus_tag="VFO88_08690"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9545642.1"
                     /translation="MKGAVAAGHPLTAEAGVRALEEGGNAVDACVAAGLVSWVTESPL
                     TGPGGGGFMLVHRAHDRSDRLLDFFVSVPGRGFAGPLRAAEPVDVAFEPTNIQVFHVG
                     GPSCAVPGMIAGLTAAHRSYGRRPWSELVAPALELARDGIEVTGAQASLHRILDPILR
                     RTPGGRALYGEDAPLPPGGRLVLAELAATLERLAAEGAEPFYRGDLARRTSEAVLEDG
                     GTLTKDDLAGYRVVRRTPVRARFRGHELVSNPPPSSGGTLVAFALGVLDRLGPPDEPG
                     SARQIARLAEVMREATRARGRRFMRDLHRGGLARRLLSDERIEESVARAEERRREAVG
                     EPAGLPSTTHVSVVDAAGNAASLSGSTGAGSGLVVPGTGIHLNNMLGEDDLLTLGLPG
                     PVGRRLTSMMAPTLVLREGEPRLVLGSAGSARLRGAIVQTVVNVLDHGLGIEEAVAAP
                     RVHLHDDLLHLEAGVDAAVSDELEAMGYDTVCWKAHNIFFGGASVVARSETGELEAAG
                     DPRRGGAGAVSA"
     gene            complement(2355..3749)
                     /gene="ftsZ"
                     /locus_tag="VFO88_08695"
     CDS             complement(2355..3749)
                     /gene="ftsZ"
                     /locus_tag="VFO88_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9545643.1"
                     /translation="MREGPLAELFRATEAAQRQAEREREAGPAADAPTVEHVDPETEP
                     ADAEAQRAEPEAGAPATEPESAPEPAHAPAQPPVPAQTVTPTSAEPRPAARFADPSLH
                     VARSTPRPESTAYLAVIRVVGVGGGGLNAVNRMIEAGISQVEFIAVNTDIQQLRGCEA
                     PVKLHIGRELTQGLGSGADPDVGRQAAEEAYDQLKHVLRGSDMVFITAGEGGGTGSGA
                     APVIARIARELGALTVGIVTTPFRFEGSRRRQQAQHGVDSLRSVCDTVIVIPNDRLLE
                     VLDQTTSMLEAFRVADDVLRQGVQGICDLITTPGLINLDFADVRTIMSEAGTALMGIG
                     FATGENRGREAAERALRSPLIDTEIVGAKGILLSIAGGQDLTLLEVNAAAETIRAAST
                     EETNIIFGATIDERLTGQVWVTVIATGIGVERRLLPQREPARLGAAPARGSSPEEPGR
                     PDDTLEPPGFLFER"
     gene            complement(3742..4518)
                     /locus_tag="VFO88_08700"
     CDS             complement(3742..4518)
                     /locus_tag="VFO88_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9545644.1"
                     /translation="MGQVIALANQKGGVAKTTTTLNLAVALKELGYTVLAVDLDPQGN
                     LTMSQGMDPDTVERSMFDVLVHSLPIDEVIQRAEIDVAVSSIDLAGAELALSSMIGRE
                     RALQKALLPIRSSYDYLMIDTPPSLGLLTINALTAADSVIVPVQCEYLSLRGLVQLEG
                     TLTMIRENLNPTVEIRGILPTMFDARTVHAREAIEMLKENFGELVFDTRIRKTVRYAE
                     APVQGASVLKYDPSGPAADAYRQLAKEVLNGAKPRTRQHA"
     gene            complement(4707..5159)
                     /locus_tag="VFO88_08705"
     CDS             complement(4707..5159)
                     /locus_tag="VFO88_08705"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HET9545645.1"
                     /translation="MRRDGGTRERTDIQRAALVFGAVFLLVTILGFIPGITTDYDRLT
                     TFDDVGAEIFGIFGVNILENIVHLLYAIAGFAAAASFVASRNYFIWGGAVYLVVWLYG
                     ILIDLESSANFLGVNTAANWLHFLLGVVMLAVGFLLGRRDYERRAAAA"
     gene            complement(5245..5505)
                     /locus_tag="VFO88_08710"
     CDS             complement(5245..5505)
                     /locus_tag="VFO88_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545646.1"
                     /translation="MDEARQVLERLERIDRLRAGGGQRGAILAEVRLLLEEGEAWLAA
                     EPAGTAAAREALDACRLRLVAGAERRADPPPPAPEGPLPRAA"
     gene            complement(5583..>5913)
                     /locus_tag="VFO88_08715"
     CDS             complement(5583..>5913)
                     /locus_tag="VFO88_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545647.1"
                     /translation="LALAACGGGGAEEGSTPPARSVPPSLVQEAEERGTVLVVVALDA
                     RGRAEIAAVQDELLASLGGHGEVAGRPERIPQLAVRVDAEGLEILSRSPSVVAVEPNE
                     PEPAGGS"
BASE COUNT          767 a         2198 c         2164 g          784 t
ORIGIN      
        1 ccggtcctcg tcgcgcggct cgcggacgac cacgaccacc cagtcctcgc ggtcgggaag
       61 cgggtcagcg atgcacggcc cgtcggagaa gtcgcgcccg ctctcctcct cggcagcgta
      121 ggcgtcccgc gcgtcgttca gcaccgcctg cgcttcctcc agctcggatt cgcccccgca
      181 gcccgccgcg acgggcagga gcgcgagcgc ggccagcacc gctccgacca cgacgatcct
      241 catcagacct ccttcgccat gagcacggcg tccacgaggt cggcgccgcg ccggtagtgg
      301 ttgcgccgaa cgccctcgcg ctcgtacccg agcgactcgt agagcgcgat cgcggcatcg
      361 ttgttcggca ggacgtgcag ctcgagcttg cggacgccgg cgcctcgcgc ccactcctcc
      421 gccccgagca tgagcgcccg gccgacgccg cgccggcgcc agtccgcggc gaccatgagg
      481 ccgaggtcgg ccacgtggcg gctcgccggg tgcgggtcgc gcgagagcga gagacggccg
      541 acgatgccct cgggcgcctc cgcgacgagg acggccgccg ccttcgaccg gcggatcgcg
      601 cgcacgtagc ggcgctcgtc gctcacgctc cgccaggtcc cgttcgtgac gagccagcct
      661 tccggctcgg agccgaccgc gagcgcgagc tcgacgagct cggcggcgtc gccgggctcg
      721 gccttgcgca cgtggaaagg cggccgctcg ctcatgcgct caccgccccc gccccgccgc
      781 ggcgcgggtc gcccgccgcc tcgagctcgc ccgtctcgct gcgcgccacg acggacgcgc
      841 ctccgaagaa gatgttgtgc gccttccagc agacggtgtc gtagcccatg gcctcgagct
      901 cgtccgagac ggccgcgtcg acgccggcct cgaggtggag gaggtcgtcg tgcaggtgga
      961 cgcgcggcgc ggcgaccgcc tcctcgatcc cgaggccgtg gtcgaggacg ttcacgaccg
     1021 tctggacgat cgcgccgcgc aaccgagccg atccggcgct cccgagcacg agccgcggct
     1081 cgccctcgcg gagcacgagg gtcggcgcca tcatgctcgt cagccgccgc ccgacgggcc
     1141 cgggcaggcc gagcgtcagc aggtcgtcct cgccgagcat gttgttcagg tggatcccgg
     1201 tcccgggcac gacgagcccg gagcccgccc cggtcgagcc ggagagcgag gcggcgttcc
     1261 cggccgcgtc gacgacgctc acatgggtcg tcgacggcag gcccgccggc tcgccgaccg
     1321 cctcgcgccg ccgctcctcg gcgcgcgcga cgctctcctc gatccgctcg tccgagagga
     1381 gccgccgcgc gaggccgccg cggtggaggt cgcgcatgaa cctccgcccg cgggcgcggg
     1441 tcgcctcgcg catgacctcg gcgaggcgcg cgatctgacg ggcgctcccc ggctcgtccg
     1501 gagggccgag gcggtcgagg acgccgagcg cgaacgcgac gagcgtcccg cccgacgagg
     1561 gcggcgggtt cgacacgagc tcgtgccccc ggaagcgcgc ccgcaccggc gtgcgccgga
     1621 cgacgcggta gcccgcgagg tcgtccttcg tgagcgtccc gccgtcctcg agcacggcct
     1681 cgctcgtccg gcgcgcgagg tcgccccggt agaacggctc ggcgccctct gcggcgaggc
     1741 gctcgagcgt cgccgcgagc tccgcgagca cgagcctccc gcccggcgga agcggcgcgt
     1801 cctcgccgta gagcgcgcgc ccgcccggcg tccggcgcag gatcgggtcg aggatccggt
     1861 ggaggctcgc ctgcgccccg gtcacctcga tcccgtcgcg cgcgagctcg agcgcgggcg
     1921 cgacgagctc gctccacggc cgccgcccgt acgagcggtg cgcggcggtg agcccggcga
     1981 tcatccccgg cacggcgcac gacgggccgc cgacgtggaa gacctggatg ttcgtcggct
     2041 cgaacgcgac gtcgaccggc tcggccgcgc ggagcgggcc ggcgaacccg cggcccggga
     2101 ccgagacgaa gaagtcgagc agccggtcgc tgcggtcgtg cgcccggtgg acgagcatga
     2161 acccgccccc gcccgggccg gtgagcgggc tctcggtcac ccaggacacg agcccggcgg
     2221 cgacgcaggc gtcgacggcg ttcccgccct cctcgagcgc ccgtacgccc gcctcggcgg
     2281 tcagcggatg cccggcggcg acggcgccct tcatgcggtt gagggtatcg cggctcggcg
     2341 cgacgctccc agggtcagcg ctcgaacagg aagccgggcg gctcgagggt gtcgtcggga
     2401 cgcccgggct cctccggaga cgaaccacgg gccggcgccg caccgagcct cgccggctcg
     2461 cgctgcggca agagcctgcg ctccacgccg atcccggtcg cgatcacggt cacccacacc
     2521 tggccggtca gccgctcgtc gatcgtcgcg ccgaagatga tgttcgtttc ctccgtcgac
     2581 gccgcgcgga tcgtctccgc ggccgcgttc acctcgagca gcgtgaggtc ctgcccgccc
     2641 gcgatcgaga ggaggatgcc cttcgcgccg acgatctccg tgtcgatcag cggtgagcgg
     2701 agcgcccgct cggccgcctc gcgcccgcgg ttctcgccgg tcgcgaagcc gatccccatg
     2761 agcgcggtgc cggcctcgct catgatcgtg cgcacgtccg cgaagtcgag gttgatgagc
     2821 ccgggcgtcg tgatcaggtc gcagatgccc tggacgccct gccggaggac gtcgtcggcg
     2881 acgcggaacg cctccagcat cgaggtcgtc tggtcgagca cctcgagcaa ccggtcgttc
     2941 gggatcacga tcaccgtgtc gcagaccgag cggagcgagt cgacgccgtg ctgcgcctgc
     3001 tggcggcgcc gggagccctc gaacctgaac ggcgtcgtga cgatcccgac ggtgagcgcg
     3061 ccgagctcgc gcgcgatccg cgcgatgacg ggcgccgcgc cggagccggt gccgcctccc
     3121 tcgccggccg tgatgaagac catgtccgaa ccgcggagca cgtgcttgag ctggtcgtac
     3181 gcctcctcgg cggcctggcg cccgacgtcc gggtcggcgc cggagccgag gccctgcgtg
     3241 agctcccggc cgatgtggag cttcaccggc gcctcgcagc cgcggagctg ctgtatgtcc
     3301 gtgttcaccg cgatgaactc gacctgcgag atcccggcct cgatcatccg gttgaccgcg
     3361 ttcaggccgc cgccgccgac gccgacgacg cggatcaccg cgaggtaggc ggtcgactcg
     3421 gggcgcggcg tggagcgcgc cacgtggagc gaggggtcgg cgaagcgcgc cgcggggcgc
     3481 ggctcggccg aggtcggagt gaccgtctgc gcggggaccg gcggctgcgc cggggcgtgc
     3541 gccggctcgg gcgccgactc gggctccgtc gccggagcgc cggcctcggg ctcggcccgc
     3601 tgcgcctccg cgtcggccgg ctccgtctcg ggatcgacgt gctcgacggt cggggcgtcg
     3661 gcggccggtc cggcctcgcg ctccctctcc gcctgccgct gggcggcctc cgtcgcgcgg
     3721 aagagctccg cgagcgggcc ctcacgcatg ctggcgcgtg cgcggtttcg cgccattgag
     3781 cacctccttt gcgagctgcc ggtaggcgtc cgccgccggg ccgctcgggt cgtacttgag
     3841 gacggaggcg ccctggacgg gagcctcggc gtagcggacc gtcttgcgga tccgcgtgtc
     3901 gaagacgagc tcgccgaagt tctccttcag catctcgatc gcctcgcgcg cgtgcacggt
     3961 gcgcgcgtcg aacatcgtcg ggaggatccc gcggatctcg acggtcgggt tcaggttctc
     4021 gcgaatcatc gtgagcgtgc cctcgagctg gacgagcccg cggagcgaca gatactcgca
     4081 ctggacgggg acgatgacgc tgtccgccgc cgtcagcgcg ttgatcgtga gcagccccag
     4141 cgagggcggg gtgtcgatca tgaggtagtc gtacgagctg cggatcggga ggagcgcctt
     4201 ctggagcgcg cgctcgcggc cgatcatgct cgagagcgcg agctcggcgc ccgcgaggtc
     4261 gatcgaggag acggcgacgt cgatctcggc gcgctggatc acctcgtcga tcgggagcga
     4321 gtggacgagc acgtcgaaca tcgaacgctc gacggtgtcg gggtccatcc cctggctcat
     4381 cgtgaggttg ccctgcgggt cgaggtcgac cgcgagcacg gtgtagccga gctccttgag
     4441 cgcgaccgcg agattgaggg tcgtcgtggt cttggccacg ccacccttct ggttggccag
     4501 ggcgatgacc tgtcccaagg ttgctccttg cgaggcgggc gttcgctcgc gcgaacgcgt
     4561 caagtttccc agaaggctca cagggccttc atttcgggtg aggctaccac aatcctcctg
     4621 cctcccagat tgccgcaaat tgcggaaaaa ggaaagggcg ggttgagacg cccgccctct
     4681 cccgcgagac cgctccgaga ccggcgttac gccgccgcgg cgcggcgctc gtagtcgcgg
     4741 cgcccgagca ggaacccgac ggcgagcatc acgacgccga gcaggaagtg gagccagttc
     4801 gcggcggtgt tcacgccgag gaagttcgcc gagctctcga ggtcgatcag gatcccgtag
     4861 agccagacga cgaggtacac cgcaccgccc cagatgaagt agttgcgcga cgcgacgaag
     4921 ctcgcggcgg ccgcgaaccc cgcgatcgcg tagagcaggt ggacgatgtt ctcgaggatg
     4981 ttgacgccga agatcccgaa gatctcggcc cccacgtcgt cgaacgtcgt caaccggtcg
     5041 tagtccgtcg tgatgcccgg gatgaacccg agaatcgtga cgaggaggaa gacggcgccg
     5101 aacacgagcg ctgcgcgctg gatgtcggtc cgctccctcg ttccgccgtc cctccgcatc
     5161 ggtccctccc ttcggatggg gtcgccggtt cggtcaccgg cacctgccga tcgcctatcc
     5221 cggtcgcctc ggtcgcaaac ggggtcaggc ggcgcgcgga agcgggcctt ccggagcggg
     5281 cggcggcggg tcggcgcggc gttcggcgcc cgcgacgaga cggagccggc aggcgtcgag
     5341 cgcctcgcgg gcggcggccg tcccggccgg ctccgccgcg agccaggctt ccccttcctc
     5401 caggagcagg cgaacctcgg cgaggatcgc gccccgctgc ccgccgccgg cgcggagccg
     5461 gtcgatccgc tcgagccgct cgagcacctg ccgtgcctcg tccatgccga acaccgtagg
     5521 gcggaaaggc gcacgagggc gtgacgcttc gtgccgaaag cgtcacggaa gcgtgacgat
     5581 tctcaggagc cgccggccgg ctcgggctcg ttcggctcga cggcgacgac cgacggcgag
     5641 cgcgagagga tctcgaggcc ttccgcgtcg acccgcacgg cgagctgcgg gatccgctcg
     5701 ggccggcccg cgacctcgcc gtgcccgccg agcgaggcga gcagctcgtc ctgcacggcg
     5761 gcgatctcgg cgcgcccgcg cgcgtcgagc gcgacgacga cgagcacggt tccccgctcc
     5821 tccgcctcct ggacgaggct cggcgggacc gaccgcgccg gcggcgtcga gccctcctcc
     5881 gccccgccgc ctccgcaggc ggcgagcgcg agc
//
LOCUS       DASTFC010000294         6100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_172426,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000294 DASTFC010000000
VERSION     DASTFC010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172426"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFO88_08720"
     CDS             <1..252
                     /locus_tag="VFO88_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9545648.1"
                     /translation="HGSAPKYTGQNKVNPMAMMLSGVMMLRHLEERDAAQRLEDAIAA
                     VIAEGRSVTYDMKPTRDDPTAVGTSEVADAIINEMGVRV"
     gene            249..1178
                     /gene="mdh"
                     /locus_tag="VFO88_08725"
     CDS             249..1178
                     /gene="mdh"
                     /locus_tag="VFO88_08725"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9545649.1"
                     /translation="MRRKVTVVGAGNVGATAAQEIARRDYAAVVLVDIKEGLPQGKAL
                     DIDQAGPVLGYEPTVRGSNGYEETAGSDVVVITAGLPRKPGMSRDDLVTTNEEIVRSC
                     ASAAAEQSPDAILVIVSNPLDAMCHVARNVTGWPRERVVGMAGILDTARFRTFIAWET
                     GSSAKDVTAMVLGGHGDQMVPVVSATTVGGIPLTKLVAADRIEQMVERTRKGGGEIVE
                     LLGTSAWYAPGAAAAEMVDAICLDQKRILACTAYLEGEYGIDGLYMGVPVKLGARGVE
                     EIVELDLTDAERAELEASSAAVREVVGVLQHGG"
     gene            1200..2189
                     /locus_tag="VFO88_08730"
     CDS             1200..2189
                     /locus_tag="VFO88_08730"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET9545650.1"
                     /translation="MKRLLALAAVLAVAAGVLYVVPSDHYLFLPDRARPVDALVSIEE
                     EELEADDGTTDEGVYMVDILVRRASLLERLIPELADGATLVPGHAVNPIGVSDTQRRQ
                     TSLNQMSRSQEIAVAVALEALGYVVDVEDEGAEVTTVLPDRPADGILEVGDIVIGAAG
                     TPVRRARQLQAALADVRPGEEVGLTIVRDGEEMKVTVGTMPSEDDPNRAVMGVLIEQA
                     ATFDFPLEIEIDAGDIGGPSAGLAFALGITDELGDELTCGRRITVTGELDLEGNVGAI
                     GGVKQKAIGADQSGADIFVVPEGNAVEAREHAPEELEVIAVSTYDEAVDALEC"
     gene            2283..2468
                     /locus_tag="VFO88_08735"
     CDS             2283..2468
                     /locus_tag="VFO88_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545651.1"
                     /translation="MTSPGDCGADMPTCDDCYFRRELLCALSGNTICPTFRAAEQAPP
                     LYAVLAEPEPERRPVAA"
     gene            complement(2478..3578)
                     /locus_tag="VFO88_08740"
     CDS             complement(2478..3578)
                     /locus_tag="VFO88_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9545652.1"
                     /translation="MDVVSFARGIPGPDLLPAEELGQCALAAARKDGARALNYGPPSG
                     YPPLREWVAARHGVAPERVILTTGSLQGFNFVARELVRRDATVFVEAPCYDRSLGILR
                     SLGARTVAIPVGEDGIDVEGLAVALEATPGEKLVYTIPTFQNPSGHTLGRAGRERLLE
                     AARATGAVVLEDDPYGLLRFEGESLPQLFELSGGEGAIFLSSFSKTVSPGIRVGYVVL
                     PEELVGAIERQVLENYVSPSIFAQAALAEFVHEGYFEPNLERAREGLRIRRDAMLDAL
                     EAEAPEGVAWNRPEGGYFLWLDLPDGVGAAALLERGNEAGVTFVKGSDFYLDGGGSNA
                     ARLAFSFATAEEIGVGVERLAGLVREAAGVAA"
     gene            3783..5510
                     /locus_tag="VFO88_08745"
     CDS             3783..5510
                     /locus_tag="VFO88_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9545653.1"
                     /translation="MKASIAAGAGLALPLRTGALGAGTAAAAVGATTPVTLGLEPFVD
                     ALPVPPVWSGDELALRGLEMAESTHRFHRDLGPVPTWGYGGASYLGPTIHATVGEDVR
                     FLARNRLGQHPLGVDTELHGPDMADDAEAPRVSLHLHGGYTCPHSDGYPEDTFTPGQD
                     KMYHYFNAQQAGTTWYHDHALGITRLNVYAGLAGFYLMRDAAAESGLPSGEFEIPIAI
                     QDKTFGRAEDGSLALHYPNPWAPEFFGDTAVVNGKAWPYLEVARGLYRFRLLNGSSSR
                     IYNLFLEPGIPWFLIGTDTGFVERPVPLRNVVLAPGERADVVVDFRSRRAGDRLRLVN
                     KPLPPHVVSPEDVGIPELVEFRVTGATGFRRGLPGRLAAPVKLPDRGVPERTVLLVEI
                     MDPETDEPVMALLNNRPWETEEIERPVVDTTEKWNIVNLTADTHPIHLHLVQFRGLWR
                     RGFDAERYLTDVFGTDMLTPADVGTGTRPFPDPTPYLAGPARKPERWEDGWKDTIQAH
                     PGTVTQILVPFGPGAFPGVPFGLAGRVTPMPGHDCDHRIPFTGEYVWHCHILDHEDNE
                     MMLPYEVVR"
     gene            5561..>6100
                     /locus_tag="VFO88_08750"
     CDS             5561..>6100
                     /locus_tag="VFO88_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545654.1"
                     /translation="MRGAALAAIAAAAALAPAAAAAPAQPEQRGREVTALSRNLYVGF
                     DVTTVLGATTPAEFVTRATQSWLSVVASDPESRAEAWADEIAATRPDVVGLQEAALFR
                     IDTPSDGPATPAEEVRFDYVALLLDALERRGLSYDVASSLEGSDIEVPTALGFDVRLT
                     DRIVLLVRADAQGGKLRVLR"
BASE COUNT          889 a         2204 c         2218 g          789 t
ORIGIN      
        1 cacggctcgg cgccgaagta cacgggccag aacaaggtga acccgatggc gatgatgctc
       61 tcgggcgtga tgatgctccg ccacctcgag gagcgcgacg cagcgcagcg gctcgaggac
      121 gcgatcgccg ccgtgatcgc cgagggccgg agcgtgacgt acgacatgaa gccgacccgc
      181 gacgacccga ccgccgtcgg gacgagcgag gtcgcggacg cgatcatcaa tgagatggga
      241 gtcagggttt gagacgcaag gtgacggtgg tcggggcggg gaacgtcggc gcgaccgcgg
      301 cgcaggagat cgcgcgccgc gactacgcgg cggtcgtcct ggtggacatc aaggagggcc
      361 tcccgcaggg caaggcgctc gacatcgacc aggcgggccc ggtgctcggc tacgagccga
      421 cggtgcgcgg ctcgaacggc tacgaggaga ccgccggctc ggacgtcgtc gtcatcacgg
      481 ccggcctccc gcgcaagcct gggatgagcc gcgacgacct cgtgacgacg aacgaggaga
      541 tcgtccgctc gtgcgcgagc gcggccgcgg agcagagccc ggacgcgatc ctcgtgatcg
      601 tctcgaaccc gctcgacgcg atgtgccacg tcgcgaggaa cgtgaccggc tggccgcgcg
      661 agcgcgtcgt gggcatggcc gggatcctcg acacggcccg cttccgcacg ttcatcgcct
      721 gggagacggg ctcgtccgcg aaggacgtga ccgcgatggt gctcggcggc cacggcgacc
      781 agatggtgcc ggtcgtctcg gcgacgacgg tcggcgggat cccgctcacg aagctcgtcg
      841 cggccgaccg gatcgagcag atggtcgagc gcacgcgcaa gggcggcggc gagatcgtcg
      901 agctgctcgg cacgtccgcc tggtacgcgc cgggcgcggc cgcggccgag atggtcgacg
      961 cgatctgcct cgaccagaag cgaatcctcg cctgcaccgc gtatctcgag ggcgagtacg
     1021 ggatcgacgg gctctacatg ggcgtcccgg tgaagctcgg tgcgcgcggc gtggaggaga
     1081 tcgtcgagct cgacctgacc gacgccgagc gcgccgagct cgaggcgtcg tcggcggccg
     1141 tccgcgaggt cgtcggcgtc ctgcagcacg gcggctgagg cgcccgcgac aatggcggcg
     1201 tgaaacgcct gctcgcgctc gccgcggtgc tcgccgtcgc ggcgggcgtc ctctacgtgg
     1261 tcccctccga ccactacctc ttcctgcccg accgggcgcg ccccgtcgac gcgctcgtgt
     1321 cgatcgagga ggaggagctc gaggccgacg acggcacgac ggacgagggc gtctacatgg
     1381 tggacatcct cgtgcgccgc gcctcgctcc tcgagcgcct catccctgag ctcgcggacg
     1441 gggcgacgct cgtccccggc cacgccgtca acccgatcgg cgtgagcgac acccagcggc
     1501 gccagacgag cctcaaccag atgtcccgct cgcaggagat cgcggtcgcc gtcgccctcg
     1561 aggcgctcgg ctacgtcgtg gacgtcgagg acgagggcgc ggaggtgacg acggtgctcc
     1621 cggaccggcc ggcggacggg atcctcgagg tcggcgacat cgtgatcggc gccgccggca
     1681 cgccggtgcg tcgcgcgcgc cagctccagg ccgccctcgc cgacgttcgg cccggcgagg
     1741 aggtcgggct cacgatcgtc cgcgacggcg aggagatgaa ggtcacggtc gggacgatgc
     1801 cgtccgagga cgacccgaac cgcgccgtca tgggcgtgct gatcgagcag gcggcgacgt
     1861 tcgacttccc gctcgagatc gagatcgacg ccggtgacat cggcgggccg tcggcggggc
     1921 tcgcgttcgc cctcgggatc acggacgagc tcggcgacga gctcacgtgc ggccgccgga
     1981 tcacggtcac gggcgagctc gacctcgagg gcaacgtcgg ggcgatcggc ggcgtgaagc
     2041 agaaggcgat cggcgccgac cagtccgggg cggacatctt cgtcgtcccc gaggggaacg
     2101 ccgtcgaggc ccgcgagcac gcgcccgagg agctcgaggt gatcgcggtc tccacgtacg
     2161 acgaggcggt cgacgccctc gagtgctgac acgttggcac cgttgacact tcgcggaatt
     2221 tgcgggctta tcggctcggg cggggctggt agcgcggcgg cgccggacct atcatcgcgc
     2281 cggtgacctc ccccggcgac tgcggcgcgg acatgcccac ctgcgacgac tgctacttcc
     2341 gtcgcgagct gctctgcgcc ctctcgggga acacgatctg cccgaccttc cgcgccgccg
     2401 agcaggcgcc gcccctctac gcggtgcttg cggagccgga gcccgagcgc cggcccgtcg
     2461 ccgcgtagcg cgaacggcta ggccgcgacg ccggcggcct cgcggacgag acccgcgagc
     2521 cgctcgacgc ccacgccgat ctcctccgcc gtggcgaacg agaacgcgag ccgggcggcg
     2581 ttcgacccgc cgccgtcgag gtagaagtcc gaccccttca cgaacgtgac gcccgcctcg
     2641 ttcccgcgct cgagcagcgc ggccgcgccg accccgtccg gcaggtcgag ccagaggaag
     2701 tagccgccct ccggccggtt ccaggcgacc ccctccggcg cctctgcctc gagcgcgtcg
     2761 agcatcgcgt cgcgccggat gcggaggcct tcgcgcgcgc gctcgaggtt cggctcgaag
     2821 tagccctcgt gcacgaactc ggcgagcgcg gcctgggcga agatcgacgg cgagacgtag
     2881 ttctcgagca cctgccgctc gatcgcgccg acgagctcct ccggcaggac gacgtagccg
     2941 acgcgaatcc ccggggagac cgtcttcgag aacgagctca ggaagatcgc gccctcgccc
     3001 ccggagagct cgaagagctg cggcagcgac tcgccctcga agcgcaggag cccgtacggg
     3061 tcgtcctcga ggacgaccgc accggtcgcg cgcgcggcct cgagcagccg ttcgcgcccg
     3121 gcgcgcccga gcgtgtggcc gctcgggttc tggaacgtcg ggatcgtgta gacgagcttc
     3181 tcgcccggcg tcgcctcgag cgcgaccgcg agcccctcga cgtcgatccc gtcctcgccg
     3241 acggggatcg cgaccgtgcg cgcgccgagc gagcgcagga tgccgagcga gcggtcgtag
     3301 cagggcgcct ccacgaagac ggtcgcgtcg cggcggacca gctcccgggc cacgaagttg
     3361 aagccctgga gcgacccggt cgtcaggatc acgcgctccg gcgcgacgcc gtgccgcgcg
     3421 gcgacccact cgcggagcgg cggatagccc gacggcggcc cgtagttgag cgcgcgggcc
     3481 ccgtccttcc gcgccgcggc gagcgcgcac tgcccgagct cctccgcggg gaggaggtcg
     3541 ggaccgggga tgccgcgcgc gaacgagacg acgtccatgg ccccgatact agtgacggga
     3601 cccgactacc ccgccggccc tcgggctccg ccgccccgat gccgcgaaat cggtagttcg
     3661 ccgtagccga atggggcgac gcccggatgg agacgcgatt cgcgcacgcg acgatccgcg
     3721 cactgcggga cgcggcatcg aaaggagcca gggtcatgac gggactgacg agaaccgatt
     3781 tcgtgaaggc gagcatcgca gcgggcgcgg ggctcgcgct tccactccga acgggcgcgc
     3841 tcggagcggg gaccgcggcc gccgccgtgg gcgccacgac gcccgtgacg ctcggcctcg
     3901 agcccttcgt cgacgcgctg cccgtgccgc cggtctggtc gggcgacgag ctcgccctgc
     3961 gcgggctcga gatggccgag tcgacccacc gcttccaccg cgacctgggc ccggtgccga
     4021 cgtgggggta cggcggcgcg tcgtatctcg ggccgacgat ccacgcgacg gtcggcgagg
     4081 acgtgcgctt cctcgcgcgc aaccggctcg ggcagcatcc gctcggcgtc gacacggagc
     4141 tgcacgggcc cgacatggcg gacgacgccg aggccccgcg cgtctccctt cacctgcacg
     4201 gcggctacac ctgcccgcat tccgacggct acccggagga cacgttcacg ccgggccagg
     4261 acaagatgta ccactacttc aacgcccagc aggccgggac gacgtggtac cacgaccacg
     4321 cgctcgggat cacgcggctc aacgtctacg cgggtctcgc gggcttctac ctcatgcgcg
     4381 acgcggccgc cgagtccggc cttccgagcg gtgagttcga gatcccgatc gcgatccagg
     4441 acaagacctt cgggcgcgcc gaggacgggt cgctcgcgct gcactacccg aacccctggg
     4501 cgcccgagtt cttcggcgac accgcggtcg tgaacgggaa ggcgtggccg tatctcgagg
     4561 tcgcgcgcgg cctctaccgc ttccgcctcc tgaacggctc gagctcgcgg atctacaacc
     4621 tgttcctcga gcccggcatc ccctggttcc tgatcggcac ggacacgggc ttcgtcgagc
     4681 ggccggtgcc gctcaggaac gtcgtgctcg cacccggcga gcgcgcggac gtcgtcgtcg
     4741 acttccggtc acgacgcgcg ggtgaccgcc tgcggctcgt caacaagccg cttccgccgc
     4801 acgtcgtgag cccggaggac gtcgggatcc ccgagctcgt cgagttccgc gtcacggggg
     4861 cgaccggctt ccggcgcggc ctccccgggc ggctggctgc gccggtgaag cttcccgacc
     4921 ggggcgtgcc ggagcggacg gtgctcctcg tcgagatcat ggacccggag accgacgagc
     4981 cggtcatggc cctcctcaac aaccggccgt gggagacgga ggagatcgag cgcccggtcg
     5041 tggacacgac cgagaagtgg aacatcgtga acctgacggc cgacacgcac ccgatccacc
     5101 tgcacctcgt gcagttccgg gggctctggc ggcgcggctt cgacgcggag cgctacctga
     5161 ccgacgtctt cgggacggac atgctcacgc ccgccgacgt cggcacggga acgcggccct
     5221 tcccggatcc gacgccctac ctcgcgggcc cggcccgcaa gccggagcgc tgggaggacg
     5281 gctggaagga cacgatccag gcgcacccgg ggacggtgac gcagatcctc gtcccgttcg
     5341 ggcccggcgc cttcccgggc gtcccgttcg gcctcgcggg ccgggtgacg ccgatgccgg
     5401 gccacgactg cgaccatcgg atcccgttca cgggcgagta cgtctggcac tgccacatcc
     5461 tcgaccacga ggacaacgag atgatgctcc cctacgaggt ggtgcgctga agagggtcta
     5521 gtcagcaggc aacggctgtg caaaccttgt gccgatgcgc atgaggggcg cggctctggc
     5581 ggcaatcgcg gcggcggcgg cactggcacc tgcggccgcc gccgcgcctg cacagcccga
     5641 gcagcgcggc cgcgaggtca cggcgctctc gcggaacctc tacgtcggct tcgacgtcac
     5701 gaccgtgctc ggggcgacga cgccggccga gttcgtcacg cgtgcgacgc agagttggct
     5761 cagcgtcgtc gcctccgacc cggagagccg cgccgaggcg tgggcggacg agatcgccgc
     5821 gacacgcccc gacgtggtcg ggctgcagga ggcggcgctc ttccggatcg acacgccctc
     5881 ggacggcccc gccacgccgg ccgaggaggt gcgcttcgac tacgtcgcgc tgctcctcga
     5941 cgcgctcgag cggcgcgggc tctcgtacga cgtggcgtcg tcgctcgagg gctcggacat
     6001 cgaggtgccg accgcgctcg ggttcgacgt ccggctgacc gaccggatcg tcctgctcgt
     6061 tcgcgccgac gcgcagggcg ggaagctgcg cgtcctgcgc
//
LOCUS       DASTFC010000295         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_139292,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000295 DASTFC010000000
VERSION     DASTFC010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139292"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFO88_08755"
     CDS             <1..763
                     /locus_tag="VFO88_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET9545655.1"
                     /translation="VLAGRRPLFVVANAGSTNTGAIDPLGEIAAVCAEQDAWFHVDAA
                     YGGFAILTERGRAWLDGIDEADSVTLDPHKWLYQPYECGCLVVRNGPLLSQAFHILPD
                     YLQDTAVGGVEVNFADRGLQLTRSARALKVWLSLHHFGVGAFRAAIDRTLDLAVLAEE
                     RVRASDELELLSPASLGIVCFRRRPHGGAAGERELEALNSDLARRLAESGEGLVSTTR
                     VDRRFALRMCVLGHRSRAEDIERVLAWLESAPVAP"
     gene            881..1711
                     /locus_tag="VFO88_08760"
     CDS             881..1711
                     /locus_tag="VFO88_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545656.1"
                     /translation="MRVAMPQPFDFALTTERFALFGHDPANVWAEGRLHRVLAGRETT
                     IAPAPGGADVAPPEVELAEPVRRLLGAGFDLPAFARFAADEDPVLARLAAERPGLRPA
                     LVPDPWEALVTAITAQQVSLRAASAIRARFVQAFGRRRERSWAFPTRDRVARETPDRL
                     RALGFSTRKAEYAIALARAPLDLDALAALPDHEVKAALTALPGIGEWTADWFLARHLG
                     RPSAWPAGDLALRKAVGAFYLDGRYPTIAETRAVGDRLGEHANVAAHTLLAALRRLPA
                     "
     gene            1708..2475
                     /locus_tag="VFO88_08765"
     CDS             1708..2475
                     /locus_tag="VFO88_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545657.1"
                     /translation="MTLRIRIDGPPTEDALRDVVERARAEGETEVVVETSHEAGEAWI
                     RAGFSELSRVLTARVDALEQHVGGEREASFGSIHVQSDDVDAVVRAVRQFVPRLPGGS
                     RGSVVLPPRDGWTSVYDELTDREPEMLRRLARELSDRMGAFVVAIGAEEGHVVRYVAL
                     ERGRVVDEYLSVPEFYGPLPPGEVIALGANPRLMSRLTGADAEAVRAVARTAQAPAEL
                     PPARELLAELARVFALPGAEHGYEQARAEPGAIDLPR"
     gene            complement(2464..>2765)
                     /locus_tag="VFO88_08770"
     CDS             complement(2464..>2765)
                     /locus_tag="VFO88_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET9545658.1"
                     /translation="GAFADFLPEHEELLLRVVRAAGRDRGLGDFWGHMLVARGSADAM
                     LDAAPLALWDVAALQPIVAEAGARLTALDGSPWRAPGPALCAAEPLHAELAALAR"
BASE COUNT          314 a         1087 c         1010 g          354 t
ORIGIN      
        1 cgtgctcgcg gggcggcggc cgctcttcgt cgtggcgaac gcgggctcga cgaacaccgg
       61 ggcgatcgac ccactcggcg agatcgcggc ggtctgcgcg gagcaggacg cgtggttcca
      121 cgtggacgcg gcctacggcg ggttcgccat cctgaccgag cgcggccggg cgtggctcga
      181 cgggatcgac gaggcggact cggtcacgct cgacccacac aagtggctct accagcccta
      241 cgaatgcggc tgcctcgtcg ttcggaacgg gccgctgctc tcgcaggcgt tccacatcct
      301 cccggactac ctgcaggaca ccgcggtcgg cggcgtcgag gtgaacttcg ccgaccgtgg
      361 gctccagctc acgcgctcgg cgcgggcgct caaggtgtgg ctctccctcc accacttcgg
      421 cgtgggcgcg ttccgggcgg cgatcgaccg cacgctcgac ctggccgtgc tcgcggagga
      481 gcgggtgcgg gcgtcggacg agctcgagct gctctcgccc gcgtcgctcg ggatcgtgtg
      541 cttccggcgg cggcctcacg gcggggcggc cggggagcgg gagctcgagg ccctgaactc
      601 cgacctcgcc cgccgcctcg cggagagcgg cgaggggctc gtctccacga cgcgcgtcga
      661 ccggcggttc gctctgcgga tgtgcgtgct cgggcaccgg tcgcgtgccg aggacatcga
      721 gcgcgtgctc gcctggctcg aatcggcgcc tgtcgcgccg taagggttag acgcgcgcgg
      781 gtatcccggg tcccgacccg cccgcctacc acgccgagcg tagcgccgaa aagcgcgcgg
      841 gtgtgtcacg gaccgtgccg gatatgtgac ggtttccggc gtgcgcgtcg cgatgccgca
      901 gccgttcgac ttcgccctca cgaccgagcg gttcgcgctc ttcgggcacg atccggcgaa
      961 cgtctgggcg gaggggcggc tccaccgcgt cctcgccggc cgcgaaacga cgatcgcgcc
     1021 cgcacccggc ggcgccgatg tggctccgcc tgaggtggag ctcgccgagc ccgtccgccg
     1081 gctcctcggc gccggcttcg acctcccggc gttcgcccgc ttcgccgcgg acgaggatcc
     1141 ggtgctcgca cgccttgccg cggagcgccc cggcctgcgg ccggcgctcg tcccggatcc
     1201 ctgggaggcg ctcgtcaccg cgatcaccgc gcagcaggtc tcgctccgcg ccgcgtccgc
     1261 gatccgggcg cggttcgtcc aggcgttcgg ccggcggcgc gaacggtcgt gggccttccc
     1321 gacgcgcgac cgcgtcgccc gcgagacccc cgaccggctc cgcgcgctcg gcttctcgac
     1381 ccgcaaggcg gagtacgcga tcgcgctcgc ccgcgcgccg ctcgacctcg acgccctcgc
     1441 cgcgctcccc gaccacgagg tcaaggccgc cctcaccgcc ctcccgggga tcggcgagtg
     1501 gacggccgac tggttcctcg cgcgccacct cggccgcccg agcgcgtggc cggccggcga
     1561 cctcgcgctc cgcaaggccg tcggcgcctt ctacctcgac ggccgctacc ccacgatcgc
     1621 cgagacgcgt gcggtcggcg accggctcgg cgagcacgcg aacgttgccg cgcacacgct
     1681 cctcgccgcg ctgcgtaggc tccccgcatg acgctccgga tccgcatcga cgggccgccg
     1741 accgaggacg cgctccgcga cgtcgtcgag cgcgcccgcg ccgagggcga gaccgaggtg
     1801 gtcgtggaga cgagccacga ggccggcgaa gcgtggatcc gcgcgggctt cagcgagctc
     1861 tcccgcgtcc tcaccgcccg cgtcgacgcg ctcgagcagc acgtcggcgg cgagcgggag
     1921 gcgagcttcg gctcgatcca cgtccagtcc gacgacgtgg acgcggtcgt ccgcgccgtg
     1981 cgccagttcg tcccgcggct gccgggcggc tcgcgcggga gcgtcgtcct gccgccgcgc
     2041 gacggctgga cctccgtcta cgacgagctg accgaccgcg agccggagat gctccgccgc
     2101 ctcgcccgcg agctctccga ccggatgggc gcgttcgtcg tcgcgatcgg cgccgaggag
     2161 ggccacgtcg tccgctacgt cgcgctcgag cggggccggg tcgtcgacga gtacctctcg
     2221 gtgccggagt tctacgggcc gctcccgccg ggcgaggtga tcgcgctcgg cgccaatccg
     2281 cggctcatgt cccgccttac cggcgccgac gcggaggccg tgcgggccgt ggcccgcacc
     2341 gcgcaggctc ccgccgagct tccgccagca cgcgagctcc tcgccgagct cgcccgcgtg
     2401 ttcgcgctcc cgggcgccga gcacggctac gagcaggcgc gcgccgagcc gggcgcgatc
     2461 gacctaccgc gctagcgccg cgagctccgc gtggagcggc tcggccgcgc acagcgccgg
     2521 gccgggcgcg cgccacggcg acccgtcgag cgccgtcagc ctggcgcccg cctccgcgac
     2581 gatcggctgg agcgcggcca cgtcccagag cgcgagcggc gccgcatcga gcatcgcgtc
     2641 cgcggagccg cgggcgacga gcatgtgccc ccagaagtcg ccgagccccc ggtcgcgccc
     2701 ggccgcccgc acgacgcgga ggagcagctc ctcgtgctcc ggcaggaagt ccgcgaacgc
     2761 gccga
//
LOCUS       DASTFC010000296         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1325688,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000296 DASTFC010000000
VERSION     DASTFC010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325688"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFO88_08775"
     CDS             complement(<1..774)
                     /locus_tag="VFO88_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9545659.1"
                     /translation="MATRAEPHVVALDLGGSKLAAGRVDAAGRILERRDHETETSSED
                     ALVAQLDAAIAELSGEGTSALGVGIPSLIDQRTGVPGASVNVPLAGLPLRSRLEERFG
                     LPVALENDANGAAVAEHRLGAGRGTEHMILLTIGTGIGGGLILNGRLYRGAIGAAAEL
                     GHVTVALDGPACQGTCPGIGHLEALASGTAAERLAADVAAARPESALAREPELDGRAL
                     VRLAEAGDADARGVIDTIGRRLGAGVAGYVNVFNPEVVVI"
     gene            804..1979
                     /locus_tag="VFO88_08780"
     CDS             804..1979
                     /locus_tag="VFO88_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9545660.1"
                     /translation="MAGKDKPYKVYRGGRAKGPIRPERDPSDGRRDAKSRDGAQKVAK
                     PGRRRRIVRGIVLFFAFVLLAALVWGLLGYLAVRSGVKEANDRLPEGTENALTPPNGS
                     MLTTPRSILVLGLDSGGTREDRQGTGRSDSMMVIRTDPDEHRIAYLSIPRDLRVEIPN
                     RGADKVNAAYAFGGPALAADTVEAFTGLEINHVIVVDFGTFREVIDAVGGITINNPTR
                     VRTNPFECPFKTPEECAERFDGWQFRKGEIHLDGFRALIYARTRVNQLDPGESDITRG
                     ERQQRVIQGLMSKITGFEGYLRMPLVGDDIVKPLATDLSTNELLQFAWVRRRAAADKT
                     LRCRLGGTPATIDGSSVIQSSEDNALVIAMITGETAPQRPPAGRPFEPGCFVGPAGG"
     gene            complement(1976..2257)
                     /locus_tag="VFO88_08785"
     CDS             complement(1976..2257)
                     /locus_tag="VFO88_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9545661.1"
                     /translation="MPTYEYRCPNGHTFETFHRMSDPGPEACEICGAAPLEKVLYPAA
                     IHFKGSGFYTTDYGRGSRKRDGKETDAKPAGDAGGGDKKPEKKPAETGS"
     gene            complement(2289..2507)
                     /locus_tag="VFO88_08790"
     CDS             complement(2289..2507)
                     /locus_tag="VFO88_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545662.1"
                     /translation="METDRARRLGSFLVGGVVGGVAALVAAGRVRVKDAPYERPAGDG
                     LAAFEGAPCYRELVERDDAERGDDGGRW"
     gene            2532..3341
                     /locus_tag="VFO88_08795"
     CDS             2532..3341
                     /locus_tag="VFO88_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET9545663.1"
                     /translation="MICTLGDLLLDVVVRLDGPIAEDTDTYGRTRVAPGGQAANVAAW
                     VAALGGRSRFVGVRARDAAGRMLADELARRGVEVAGPEVDEGTGTVVSLARPDGRRTM
                     LTDRGASPSLDRVDPDWLAGCSTLHVPAYSLVRSPIGAASLEAASLARAAGARVSVDL
                     SGTSAIEELGVERFRRTLAGIAPDLVFANEDEARLAGELAAPVVVVKRGARGCVVRER
                     GEEQAYEALPAEVVDTTGAGDALAAGFLVGGVELGLEAAARCVARMGAFPP"
     gene            3338..>3540
                     /locus_tag="VFO88_08800"
     CDS             3338..>3540
                     /locus_tag="VFO88_08800"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET9545664.1"
                     /translation="MTLVRLSPEVEAALAGGGPVVALETTLVSHGFPGEEGLRVGLEC
                     EKRVREAGAVPATVGVLDGAVRVG"
BASE COUNT          433 a         1318 c         1323 g          466 t
ORIGIN      
        1 gatcacgacg acctccgggt tgaagacgtt cacgtagccc gcgacgccgg cgccgagccg
       61 ccgcccgatc gtgtcgatca cgccgcgcgc atccgcgtct ccggcctccg ccagccggac
      121 gagcgcgcgc ccgtcgagct ccggctcgcg cgcgagcgcg ctctccggcc gggcggctgc
      181 gacgtcggcc gcgagccgct cggccgccgt ccccgaggcg agcgcctcca gatggccgat
      241 cccggggcag gttccctggc acgccggccc gtcgagcgcg accgtcacgt gcccgagctc
      301 cgcggcggcg ccgatcgcgc cgcggtagag ccggccgttc aggatgaggc cgccgccgat
      361 cccggtgccg atcgtgagca ggatcatgtg ctccgtccca cgccccgcgc cgagccggtg
      421 ctcggccacc gcggccccgt tcgcgtcgtt ctcgagcgcg accgggaggc cgaagcgctc
      481 ctcgaggcgg gagcggagcg gcagcccggc gagcgggacg ttcaccgacg cgcccggcac
      541 gcccgtgcgc tggtcgatga gcgacgggat cccgaccccg agcgcgctcg tgccctcgcc
      601 ggagagctcc gcgatcgcgg cgtcgagctg cgcgacgagc gcgtcctcgc tcgacgtctc
      661 ggtctcgtgg tctctccgct cgaggatccg gccggccgcg tccacccggc cggcggcgag
      721 cttcgagccg ccgaggtcga gcgcgacgac gtgtggttcc gcgcgggtgg ccatcggggg
      781 gctggctacc ataccccgtc gtcgtggccg gcaaggacaa gccctacaag gtctaccgcg
      841 gcggccgcgc gaaggggccg atccggcccg agcgcgaccc ctccgacggg cgccgcgacg
      901 cgaagtcccg ggacggcgcg cagaaggtcg cgaagcccgg ccggcggcgc cggatcgtcc
      961 gcgggatcgt cctcttcttc gcgttcgtcc tcctcgccgc gctcgtctgg gggttgctcg
     1021 gctacctcgc cgtgcgaagc ggcgtgaagg aggcgaacga ccgcctgccg gaggggacgg
     1081 agaacgcgct cacgccgccg aacggctcga tgctgacgac gccgcgctcg atcctcgtgc
     1141 tcgggctcga cagcggcggc acgcgcgagg accgtcaggg caccgggcgc tcggactcga
     1201 tgatggtcat ccgcacggac ccggacgagc accggatcgc gtacctctcg attccgcgcg
     1261 acctccgcgt cgagatcccg aaccgaggcg cggacaaggt gaacgccgcc tacgccttcg
     1321 gcggccccgc gctcgccgcg gacacggtcg aggcgttcac gggcctcgag atcaaccacg
     1381 tgatcgtcgt cgacttcggc acgttccgcg aggtgatcga cgccgtcggc ggcatcacga
     1441 tcaacaaccc gacgcgcgtc cgcacgaacc cgttcgagtg cccgttcaag acgcccgagg
     1501 agtgcgcgga gcggttcgac gggtggcagt tccgcaaggg cgagatccat ctcgacggct
     1561 tccgcgcgct gatctacgcg cgtacgcgcg tgaaccagct cgacccgggc gagagcgaca
     1621 tcacgcgcgg cgagcgccag cagcgggtca tccaggggct catgtcgaag atcaccgggt
     1681 tcgagggata cctccggatg ccgctcgtcg gcgacgacat cgtgaagccg ctcgccaccg
     1741 acctctcgac gaacgagctg ctccagttcg cgtgggtgcg ccggcgtgcg gccgcggaca
     1801 agacgctccg gtgccgcctc ggcgggacgc cggcgacgat cgacggctcg tccgtcatcc
     1861 agtcctccga ggacaacgcg ctcgtgatcg cgatgatcac cggcgagacc gcgccgcagc
     1921 gcccgcccgc gggccggccg ttcgagcccg gctgcttcgt cgggccggcc ggaggctagc
     1981 tgccggtctc ggccggcttc ttctccggct tcttgtcgcc gccgcccgcg tccccggccg
     2041 gcttggcgtc cgtctccttg ccgtcgcgct tgcggctccc gcgcccgtag tccgtcgtgt
     2101 agaacccgga gcccttgaag tggatcgcgg ccgggtagag gaccttctcg agcggcgccg
     2161 cgccgcagat ctcgcaggcc tcggggcccg ggtcggacat ccggtgaaag gtctcgaacg
     2221 tgtgcccgtt ggggcagcgg tactcgtagg taggcattgc gaaaagggcg ctgggaagcg
     2281 ggaaaagcct accagcggcc cccgtcgtca ccccgctcgg cgtcgtcccg ctcgacgagc
     2341 tcgcggtagc acggagcgcc ctcgaacgcg gccaggccgt cgcccgccgg acgctcgtac
     2401 ggggcgtcct tcacgcgcac ccggccggcg gcgacgagcg ccgcgacgcc gccgaccacg
     2461 ccgccgacga gaaacgaccc gagcctccgt gcgcgatcgg tctccaccct tcggagtcta
     2521 gggtcccgca cgtgatctgc acgctcggag acctgctcct cgatgtcgtc gtccgtctcg
     2581 acgggccgat cgccgaggac accgacacgt acgggaggac gcgcgtcgcc cccggcgggc
     2641 aggcggcgaa cgtggccgcc tgggtcgcgg cgctcggcgg gcggtcgcgc ttcgtcgggg
     2701 tgcgggcgcg cgacgcggcc ggccggatgc tcgcggacga gctcgcgcgg cgcggcgtcg
     2761 aggttgccgg gccggaggtc gacgagggca ccggcaccgt cgtctcgctc gcgcgcccgg
     2821 acgggcggcg gacgatgctc accgaccgcg gcgcctcgcc gtcgctcgac cgggtcgacc
     2881 cggactggct cgcgggctgc tcgacgctcc acgtgcccgc gtactcgctc gtccgctcgc
     2941 cgatcggcgc cgcgtcgctc gaggcggcct cgctggcgcg ggcggccggg gcgcgcgtga
     3001 gcgtcgacct ctccggcacg agcgcgatcg aggagctcgg ggtcgagcgg ttccgcagga
     3061 cgctcgccgg gatcgcgccc gacctcgtct tcgcgaacga ggacgaggcg aggctcgccg
     3121 gcgagctcgc ggcgcccgtc gtcgtcgtga aacgcggcgc gcgcgggtgc gtcgtgcgcg
     3181 agcgcggcga ggagcaggcg tacgaggcgc tcccggccga ggtcgtggac acgacggggg
     3241 cgggcgacgc gctcgcggcc gggttcctcg tcggcggggt cgagctcggg ctcgaggccg
     3301 ccgcacggtg cgtcgcccgc atgggagcct tcccgccgtg acgctcgtcc ggctctcgcc
     3361 cgaggtcgag gccgcgctcg ccggcggtgg gccggtcgtc gcgctcgaga ccacgctcgt
     3421 ctcgcacggg tttcctggcg aggaggggct gcgcgtgggg ctcgagtgcg agaagcgcgt
     3481 gcgcgaggcg ggcgcggttc ccgcgacggt gggcgtcctc gacggggccg ttcgggtcgg
//
LOCUS       DASTFC010000297         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1029318,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000297 DASTFC010000000
VERSION     DASTFC010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029318"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFO88_08805"
     CDS             complement(<1..422)
                     /locus_tag="VFO88_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545665.1"
                     /translation="MENGLLPRMYQYSRAIYRSIKDLIDPWADQETQLEYRRAVLAAC
                     EDTVERLAKDPRYFSKPERTLFQDIRRYFPITYQGQVNWAITEGIGAAVEFIEEQIES
                     GAFDGGVARCRATTRKGKPCQRTPLPEREYCPSHQHLET"
     gene            complement(579..1412)
                     /locus_tag="VFO88_08810"
     CDS             complement(579..1412)
                     /locus_tag="VFO88_08810"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET9545666.1"
                     /translation="MAGPPGDIHGPPPPPDDAAREVAESPPQQDQPVCHRCGAPHDPF
                     QEYCLECGARLTPRRAGSIWRRETWTRDSPFWFWAAFLGLLAIALITGAIVLAATRDD
                     DEPTAQTGPTTATLTLPTTGATTEPLPDTVTPPTFTGTTDVTFPTTGTTTGFTTTGFT
                     TTGFTTTTGTTTTTTQTTTTTPTGSTATWPPTRSAYTVIVGSFRESRGRGPAETRAAQ
                     ARNAGLTQVGIIRSSDFSSLRPGWYSVFTGIFDTRAEATTRLSQARATYRDAYLRYVR
                     P"
     gene            complement(1412..2008)
                     /locus_tag="VFO88_08815"
     CDS             complement(1412..2008)
                     /locus_tag="VFO88_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9545667.1"
                     /translation="MTGRFASLFARSGPPPGAPPPEAPSPGFEPSPYPAPAPGRPAPE
                     RERRYLVRLREMELRDIGGLAAEMARRDDWRYPLLHSRCAEVLALEQRIHELDALIAA
                     SALAARGVAVATCECGAPLIEGSHFCANCGRPAPETPPVATCGRCGAPLPAEANFCLV
                     CGNAVAAEAYVEPSDPSLDETVVSPRRDVDPDAERRPS"
BASE COUNT          290 a          725 c          879 g          324 t
ORIGIN      
        1 gtctcgagat gctggtgaga cgggcagtac tcgcgctcgg ggagcggggt gcgctggcac
       61 ggcttcccct tgcgggtggt ggcgcggcag cgggcgacgc cgccgtcgaa cgcgccggac
      121 tcgatctgct cctcgatgaa ctcgaccgcg gcgccgatcc cctcggtgat cgcccagttg
      181 acctggccct ggtacgtgat ggggaagtag cggcggatgt cctggaagag ggtccgctcg
      241 ggcttcgaga agtagcgggg gtcctttgcg aggcgctcga ccgtgtcctc gcaggcggcg
      301 aggacggcgc ggcggtactc gagctgcgtc tcctggtcgg cccacgggtc gatgaggtcc
      361 ttgatcgagc ggtagatcgc tcgcgagtac tgatacatgc gggggaggag tccgttctcc
      421 atcagtgcag gctcattctc tcaggggggc ggttaactgg ctgcaaacga ctgttaattt
      481 cccgttaagg gcctggttcc gatccgcagc agcgcggagt ctaaccggga tcgggcgctg
      541 ttacaaagtc ccgcccggag cagaacgcga aacttcgact aaggccggac gtagcggagg
      601 tacgcgtcgc ggtacgtcgc gcgcgcctgg gagaggcgcg tcgtggcctc cgcgcgggtg
      661 tcgaagatcc cggtgaaaac ggagtaccag ccggggcgaa ggctcgagaa gtccgacgag
      721 cggatgatcc cgacctgcgt gagcccagcg ttgcgtgcct gcgcggcccg cgtctcggcc
      781 gggccgcggc cgcgggactc gcggaacgat ccgacgatca cggtgtaggc cgagcgtgtg
      841 ggcggccacg tcgcggtcga gccggtcggc gtcgtcgtcg tggtctgcgt cgtcgtggtc
      901 gtcgtgccgg tcgtcgtcgt gaacccggtc gtggtgaacc cggttgtcgt gaagccggtc
      961 gtcgtgcccg tcgtcgggaa cgtgacgtcg gtcgttcccg tgaacgtcgg cggggtgacc
     1021 gtgtccggga gcggctccgt cgtcgcgccg gtcgtcggca gcgtgagggt cgccgtggtc
     1081 gggcccgtct gcgctgtcgg ctcgtcgtcg tcgcgggtcg ccgcgagcac gatcgcgccc
     1141 gtgatgaggg cgatcgcgag gaggccgagg aacgcggccc agaaccagaa cggggagtcc
     1201 cgggtccacg tctcccggcg ccagatcgac ccggcgcggc gcggcgtgag gcgcgcgccg
     1261 cactcgaggc agtactcctg gaacgggtcg tgcggcgcgc cgcagcgatg gcagacgggc
     1321 tgatcctgct gaggaggcga ctccgcgacc tctctggccg cgtcgtcggg agggggcgga
     1381 ggcccgtgga tgtccccggg agggccggcc atcaggaggg ccggcgctcc gcgtccggat
     1441 cgacgtctcg gcgcgggctc acgacggtct cgtcgagcga cgggtcggac ggctcgacgt
     1501 atgcctcggc cgcgaccgcg ttcccgcaca cgaggcagaa gttcgcctcg gcggggagcg
     1561 gggccccgca gcggccgcac gtggcgaccg gaggcgtctc gggcgcgggt cggccgcagt
     1621 tcgcgcagaa atgcgagccc tcgatgagcg gcgcgccgca ctcgcacgtc gcgacggcga
     1681 ccccgcgggc ggcgagcgcg ctcgcggcga tgagcgcgtc cagctcgtga atgcgctgct
     1741 cgagcgcgag cacctccgcg cagcggctgt gcaggagcgg gtagcgccag tcgtcccgcc
     1801 gcgccatctc cgccgcgagg ccgccgatgt cgcgaagctc catctcgcgg agccggacga
     1861 ggtagcggcg ctcgcgctcc ggcgcgggcc ggcccggggc cggcgcgggg tagggagagg
     1921 gctcgaagcc gggggacggc gcctcgggcg gcggcgctcc cggaggcggc ccgctgcgcg
     1981 cgaaaagcga ggcgaaacgg cccgtcatcg tgcgggcgag tctagacgat ggggcgggcg
     2041 cggcctaccg ctccgacagg acgacgaggc gctcctcggt catctcgcgg acggtgtagg
     2101 cggggccctc cttcgtgttc ccggaggcct tgatgccccc gtacggcatc tggtcggcgc
     2161 ggaaggtcgg cgcctcgttc acgacgacgc cgccgaactc gagcgcccgc gccgcggc
//
LOCUS       DASTFC010000298        16292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1062597,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000298 DASTFC010000000
VERSION     DASTFC010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 16292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16292
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062597"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VFO88_08820"
     CDS             <1..1245
                     /locus_tag="VFO88_08820"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9545668.1"
                     /translation="SQRQLAFPGCSSAYISRIEAGMRTPSLQVLHLLAQRLDVTGDYL
                     ATGGAGATAEDDVLTQAEFALRFDDLPQAERLFKEVLDSAEDARARARALDGLGQLAF
                     RKGDPRTTVERLEAALEAYGGDIGAHARLADTLGRAYAMLGDLGASVEIFDRCLGEAR
                     RRNDRLEASRFAMLLGHALIDAADFDEAQALLEETTALASDVADPLLHARLYWLESRL
                     YAEQNDAERAARYARKALSIVEMTEDRAYVGRAHQLLAHIEIDRGRPADALDVLERGR
                     SLIAEASQVEQAQYRLEEARALAALGESERAASLAMEVSGVIAGANVEDAGRSYTLLA
                     ELFASFGDSARAQELYELAAELLERNPNRYLVRVYRRHAELLESEGRADEAMDLLKKA
                     VAIGEFEQPRLRLPRRKAITHS"
     gene            complement(1232..2287)
                     /locus_tag="VFO88_08825"
     CDS             complement(1232..2287)
                     /locus_tag="VFO88_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545669.1"
                     /translation="MAETQALPEVAAAVEGISRRGLAELHTHLGGSVDPAVMWTLGHE
                     QGIALPVKDYWDFVELVSVDPRGVEGLPQLDAIYKWTELIQSSPLAVERSVHGAIGGA
                     YRKQNITTLELRFNPMKRNRGGERDLDHIIMAAARGLDLASLEYPKVRAGLILMMDRT
                     FTREQNEVIVEKAIRYRNRGIVGIDIAGPRPEPGRYPYARLAPLVETAREAGLGITIH
                     VGEEGGDTGVEEIAEVVETLRPERIGHGILAAGDEEAMRLLRDAGTVLELCPSSNLLT
                     RALADEEAVRDTVRTFLDRDVAFTVATDGPEMMRTHLRDELALLLRVGALTEDEARAA
                     NERGHEASFLAPPIRSA"
     gene            complement(2339..2473)
                     /locus_tag="VFO88_08830"
     CDS             complement(2339..2473)
                     /locus_tag="VFO88_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545670.1"
                     /translation="MASVLADRELHPAPVAKPSPVRVTAKESSITAAIARIASGRRAA
                     "
     gene            2580..3728
                     /locus_tag="VFO88_08835"
     CDS             2580..3728
                     /locus_tag="VFO88_08835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9545671.1"
                     /translation="MKIGIVVPFSWSFWGGVPEHADHQARALRGLGHDALIVMGHDPP
                     GRLTKMLHPRIGRHERPPEYVIPVGRSVIVPANGSLSNIVLTPRSMFRMERVLERERF
                     DVVHVHEPMAPILSTYALVRARCPIVTTSHSSGDTLRWYPIAHAAWGFLAERIDHRIA
                     VSERAREAAARYLGEPVEILPNGVVIPEDAHPADRLRHVVFIGRHEPRKGLPVLLEAW
                     PEIARRTGARLRVVGADPLSVRWLLRRQGVSGEHVDILGSISEDALTEELLQAKLLVA
                     PSVGRESFGMVLTRAFGCATPVVASDIEGYRDVAGPENGLLVPPGDARALAEAVVALL
                     EDEPRRLALGEAGRRLAQERYGWERIASRLLEIYGGLLDGAGEARAAA"
     gene            3725..4750
                     /locus_tag="VFO88_08840"
     CDS             3725..4750
                     /locus_tag="VFO88_08840"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9545672.1"
                     /translation="MMRPRLPRSRPGRLALLATALGAAVALVLWRLDVVVITDAFSLV
                     TWEWVAVAVVLNLASVLVRSVAWKIVVDQAIPPPPPRRRTVFAAFSIGLLGNAALPGR
                     AGELARVIVLNRRLPHRPGTWATLMGTVFTHRLFDVVVASGLVVFVIYAARIPSWARP
                     TLAIALGIGIGLLVAALVVARRTPRGGLDGLGPVRRLLGLARRGLTVLKRPAPAATAL
                     AFQAVGWATQLLAVWAMLYAFEIDLPIEAAALVLVIMNLVMVFPLWPGNVGLVQAAVA
                     LSLLTYGVSYGRGFAFGIGVHAVELGVGIALGLLFLAREGFSIAMLRQMPELREVEPD
                     QPVERVA"
     gene            4740..5723
                     /locus_tag="VFO88_08845"
     CDS             4740..5723
                     /locus_tag="VFO88_08845"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET9545673.1"
                     /translation="MSRESRRIVCAPDSLKGVLSAREAAAALAAGVREAGGEADELPL
                     ADGGEGTAEALALGPGEWRRATVSDPLGRPIEARYRLSVDGEAVVEAAEAIGLWRLAP
                     DELDPLRASSRGLGELILAAVEAGALRLTIALGGTATVDGGAGLLEVLPELPVPTVAA
                     YDVRSPLLGPRGAARMFGPQKGAGPAEVEVLEERLAASARLGPYADVPGAGAAGGLGA
                     ALASLGAELRPGLELVLTATRFRERIAGAALVVTGEGTVDASSAEGKVPAGVVDAAAE
                     AGVPVVVFGGRVEPAGADALLAAGAREVVALSGDPGRARDDLVALGARLAS"
     gene            complement(5720..5905)
                     /locus_tag="VFO88_08850"
     CDS             complement(5720..5905)
                     /locus_tag="VFO88_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545674.1"
                     /translation="MGVFDWLRGRPRDTQEERVEARAEPSEGPAGDIADPTVTGKELD
                     SAIDVRRDEEAIRHGGI"
     gene            6002..8182
                     /locus_tag="VFO88_08855"
     CDS             6002..8182
                     /locus_tag="VFO88_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9545675.1"
                     /translation="MGAHASSDGESGWTAETRLRFLAQATDLLAGSLDYEETLERVAQ
                     LAVPALADWCVVDEVRDDGELARVAVVAADTRKQELLELLQERYPPTWDSPQPAAHAV
                     RTRDAVLFAELTPEVIAANAVDEGHATLLRELEPRAAIAVPLVAHDEVIGAMSFAAAE
                     SGRRYGEDDVRFAEQLARRVALALDRARLYRSEQRARLEAEAAGERLRFLAETTAILG
                     SSLDYETTLERLARLVVPALADCCVVDVLDERRQVRQVAVAHVDPERAELVRRLEERY
                     PTSEADPRSLVGRALREATTVELPDLDVEVDEIAQDAEHAEGLRELALVSGMIVPLIA
                     RGQTLGAITLLTSTSLRRYGPDDLTLAEQLASRAAIAVDNARLYREAAERGRAARALA
                     TVADGVLLLDRDGVVRLWNPGAERITGLAAAEVLGRPARDAIPGWSEVEHALPVAHEG
                     GPAPRPLAVPLDIGGRELWLSFAGVGFEDGTVFTFRDLTEERRLEEYRSEFVATASHE
                     LRTPLAAVYGAAVTLRRGDIRLTEEEREVLLAVIADESDRLARIVNDILWASRVDSSD
                     TDVRIAPFDARQTARRVIESAQAHLPEGIEIALEAPELLPRVAADEPKLAQVLTNLVD
                     NAVKYSPDGGRIAVRLEPGRGRLRLVVEDEGLGIPAGEHERIFEKFYRLDPNQTRGVG
                     GTGLGLYICRALVERMGGRISLRSEEGEGSAFTVELPLAADVKM"
     gene            8452..8727
                     /locus_tag="VFO88_08860"
     CDS             8452..8727
                     /locus_tag="VFO88_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545676.1"
                     /translation="MGLVSWILVGVLVGLAAQRLRPRTFPLRAPGTVLAGVLGASFGG
                     FLAAATSERRIAELDLLTLAAALAGSVAVVVLVAVAGRPRDTQRERS"
     gene            8737..9714
                     /locus_tag="VFO88_08865"
     CDS             8737..9714
                     /locus_tag="VFO88_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9545677.1"
                     /translation="MVGPKDVSLFWRVFIVNAAVFAVATLVLALSPATVSWPIVAREG
                     IVLAGGLAALLVANVFLLRIALGPLERLTRLMRSIDLLKPVGRVPADGGGEVGEVIRT
                     FNDMLDRLESERRASSARVLAGQEEERRRIARELHDEIGQSLTAVLLSLKRVAAQAPA
                     DVAAELEEVQETARTSLEEIRRIARALRPGVLDDLGLVSALTALTTSFSEASGIAVER
                     RFHGPFPELPRDAELALYRIAQESLTNVARHADATSVVLTLVRRDGAIVLEIADDGRG
                     AGADGHEGGGIRGMRERALAVGGALGVESNGHGVRVTLEVPVAEGGRRA"
     gene            9711..10364
                     /locus_tag="VFO88_08870"
     CDS             9711..10364
                     /locus_tag="VFO88_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9545678.1"
                     /translation="MTAATRILLADDHTLVRRGLRLILDAEPDLEVVAEAGDGAEAVG
                     RALAVEVDLAILDVAMPRMTGLQAAQQLAKRAPKVRVLMLSMYDNEQYLFEALKAGAS
                     GYVLKSVADRDLVEACRATMRGEPFLYPGAVTALIRDHLERGRAGEEMPNDLLTPRES
                     EILKLVAEGHSSREIAETLVISVKTVERHRANILEKLGMRDRVELTRYAIRRGLVEP"
     gene            complement(10361..10591)
                     /locus_tag="VFO88_08875"
     CDS             complement(10361..10591)
                     /locus_tag="VFO88_08875"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HET9545679.1"
                     /translation="MGLGVSLFLIAIGLILWLAVSAEVSGIDINMVGLILVIIGALGL
                     LISMIFWSTWGGVGGWRRETVVRDDRYDRPPY"
     gene            complement(10671..11084)
                     /locus_tag="VFO88_08880"
     CDS             complement(10671..11084)
                     /locus_tag="VFO88_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9545680.1"
                     /translation="MARAPIDVLRRVALFEGLDEDELTRLADRFQERSVPAGQTVVEE
                     GSTGTSFFVIADGEATVSIGGEEKATFGPGDSFGEMSVIDEDVRSASVVATKDLHLFF
                     LTPWEFRPFVREQPEVAWKLLQALSRRLRAAQQRG"
     gene            11141..12220
                     /locus_tag="VFO88_08885"
     CDS             11141..12220
                     /locus_tag="VFO88_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9545681.1"
                     /translation="MTTQAAGLPAVDPALSWSEAELPERERTKHVHRLHPYLGKFVPQ
                     LAEAFLRRYARPGQLVWDPFAGSGTTLVEANALGLRAAGCDLSAFNCLLAEVKTAPYE
                     ARGLVADVAWLSVPGAEPPPAIASPYLSRWFAPAALAELLAFRARIAGTAYPGLWRVV
                     LSRAARSARLARHDDLDFPREPVRGEYFCHKHRRVCRPVGEADKFLRRYVRDAACRVR
                     EFGDSRTDAAVTVLEGDSREVDPPGPVDLVFTSPPYPGLIDYHEQHAYAFELLGLERR
                     DPDEIGRGVRGYCEDVAAVLRRARRALRRDGRVVVVVNDSRGLYDGILAAAGLRLEER
                     ILREVNRRTGRRAGAYYEEILVARP"
     gene            complement(12190..12795)
                     /locus_tag="VFO88_08890"
     CDS             complement(12190..12795)
                     /locus_tag="VFO88_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9545682.1"
                     /translation="MNGRLYDLLGRFEERSEIGHRRRSLVARLEGDVLEIGAGTGFSL
                     KHYLKASRVVALEPDRSMAARIPGKIAGAPAPVEVVDAEAESLPFPDESFDAVVSVFV
                     LCSVTDQAQALAEIHRVLRPGGTLNLLEHVRGHGRVAVWQDRLNPLQRRFFGNCHLNR
                     DTKAAVARAGFDVGEVEETRIPGSCALVRDGIYGRATRISS"
     gene            complement(12792..13019)
                     /gene="xseB"
                     /locus_tag="VFO88_08895"
     CDS             complement(12792..13019)
                     /gene="xseB"
                     /locus_tag="VFO88_08895"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET9545683.1"
                     /translation="MSEPGQAKAEPTFEEARAELEKIVAELESGRAGLEEALALWQRG
                     EELHRICLAKLDAAQGAIEELGRRAGDARPE"
     gene            complement(13016..14227)
                     /gene="xseA"
                     /locus_tag="VFO88_08900"
     CDS             complement(13016..14227)
                     /gene="xseA"
                     /locus_tag="VFO88_08900"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9545684.1"
                     /translation="MRPEPVLYGDRKVYPVSAFNRGVASWLQRLPTVWVEGEVTELRR
                     QAGWQSVFFTLKDPADGACLPVSMPRGTFDALGLDLADGATVHVFGRPELFTARGTFR
                     LRALSLEAQGLGALLAGIERLKRRLADEGLFARSRPLPVFPRRIGLLTGAEAAAKRDF
                     VTAVQARFPAARVLVAETAVQGPGAPRAIAAGLAALAAEPEIDVLVVSRGGGSFEDLL
                     PFSDERVVRAVAACPVPVVSAVGHEQDTPLCDLAADARASTPTAAARLVVPDEGELRE
                     RLASLRASLARETARTATRSRERVSALHAALGREGARTVARARERLDADAARLARAPA
                     ALVARKRAELDGLAGSLRALSPQATLGRGYAIVRSGDAVVRSGSALAPGERVDVRLAE
                     GGFGAVVEETR"
     gene            complement(14271..14486)
                     /locus_tag="VFO88_08905"
     CDS             complement(14271..14486)
                     /locus_tag="VFO88_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545685.1"
                     /translation="MEDVMDETTTVETGIDERAKVESWRLHVLAEAGYPLPLAERLAR
                     SHADLHEAVTLVTERGCAHELAAEILL"
     gene            complement(14584..15888)
                     /locus_tag="VFO88_08910"
     CDS             complement(14584..15888)
                     /locus_tag="VFO88_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545686.1"
                     /translation="METVFEAVFAVGFLVVLGSAGALLAGRIGSRALRWITGGLIVAT
                     GIAIAVAGLNVEGSFVDGSATVLAAGGLAAAAVAEAGLFVLARGLERLREEDRLLAAG
                     RQRIEDQLEEHARIRAEELEHTLARERANANHELAKQERKLAVERLDLVARQADGARA
                     ELAKSIEQVQERLEQRLAAWAADLDRGQRALETRLNELAQRQGDAIKAYEARLAADSE
                     FLRTISEEQQTALARLRTELQKVGREILEEGRAEMEAHTDERRRALAELTKRVREQEK
                     DLRGHIEREEAEAAARITSTFAEVERRQRENLERLLDRSSSRLSEESERRFDVQIRKA
                     REKSAQRLSNELEKSVEQFARQAEKDIADRIADAAQNTAARLERRLTDSAKAAESQYD
                     VSAARMSAMNERLEDALSVAERRIAELEERIEAQVATRLRDL"
     gene            complement(15899..>16292)
                     /locus_tag="VFO88_08915"
     CDS             complement(15899..>16292)
                     /locus_tag="VFO88_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9545687.1"
                     /translation="DLARLYRWLHQAARGEGPLLELTGSFAPADARYLLWILAHSADR
                     GKLDRHAGTGVLVPHKGGWVTNARHDAGLVYTRAGTAAVVVLTWTPGIAGDASDTLAA
                     DVGRVALQWLRDHRVGPHGSDAPFALWF"
BASE COUNT         1947 a         6042 c         6049 g         2254 t
ORIGIN      
        1 agccagcgcc agctggcgtt cccgggctgc tcgtccgcct acatctcgcg catcgaggcg
       61 gggatgcgca cgccctcgct ccaggtgctg cacctgcttg cccagcggct cgacgtgacg
      121 ggcgactacc tcgcgaccgg cggggccggg gcgacggccg aggacgacgt cctcacgcag
      181 gccgagttcg cgttgcgctt cgacgacctt ccgcaggccg agcggctctt caaggaggtg
      241 ctcgactcgg cggaggatgc gcgagcccgc gcccgcgcgc tcgacgggct gggccagctc
      301 gcgttccgca agggcgaccc gcggacgacg gtggagcggc tcgaggccgc gctcgaggca
      361 tacggcggcg acatcggcgc gcacgcgcgg ctcgccgaca cgctcggtcg cgcgtacgcc
      421 atgctcggcg atctcggcgc gtcggtggag atcttcgacc gctgcctggg cgaggcgcgc
      481 cggcgcaacg accggctcga agcctcgcgg ttcgccatgc tcctcggcca cgcgctcatc
      541 gacgcggccg acttcgacga ggcacaggca ctgctcgagg agacgaccgc gctcgcgagc
      601 gacgtcgcgg acccgctcct gcatgcccgc ctctactggc tcgagtctcg cctctacgcg
      661 gagcagaacg acgccgagcg cgccgcccgc tacgcgcgga aggcgctctc catcgtcgag
      721 atgacggagg accgggcgta cgtcgggcgc gcgcaccagc tgctcgcgca catcgagatc
      781 gaccggggcc gcccggctga cgcgctcgac gtcctggagc gcgggcggtc actcatcgcg
      841 gaggcgagcc aggtggagca ggcgcagtac cggctcgagg aggcgcgcgc gctcgctgcc
      901 ctcggcgagt cggagcgcgc cgcctcgctc gcgatggagg tctcgggcgt catcgccggc
      961 gcgaacgtcg aggacgcggg acgcagctac acgctgctcg ccgagctgtt cgcgagcttc
     1021 ggggactcgg cccgcgcgca ggagctctac gagctcgccg ccgagctgct cgagcgcaat
     1081 ccgaaccggt atctcgtgcg cgtgtaccgg cggcatgccg agctgctcga gagcgagggg
     1141 cgcgccgacg aggcgatgga cctgttgaag aaggccgtcg cgatcggcga gttcgagcag
     1201 ccgcgcctgc ggctcccgcg ccgcaaggcg atcacgcact cctgatcggc ggcgcgagga
     1261 agctcgcctc gtggccgcgc tcgttcgcgg cccgcgcctc gtcctcggtg agcgcgccga
     1321 cgcggaggag gagcgcgagc tcgtcccgca ggtgcgtccg catcatctcc ggcccgtccg
     1381 ttgcgacggt gaaggcgacg tcgcggtcga ggaacgtgcg cacggtgtcg cgcacggcct
     1441 cctcgtcggc gagcgcgcgc gtgaggaggt tcgagctggg acagagctcg agcaccgtcc
     1501 ccgcgtcgcg caggagccgc atcgcctcct cgtcgcccgc cgcgaggatc ccgtgcccga
     1561 tgcgctccgg ccggagcgtc tccacgacct cggcgatctc ctcgacgccc gtgtcgccgc
     1621 cctcctcgcc gacgtggatc gtgatcccga gccccgcctc gcgcgccgtc tcgacgagcg
     1681 gtgctagccg ggcatacgga tagcggcccg gctccgggcg ggggccggcg atgtcgatgc
     1741 cgacgatccc gcggttccgg taccggatcg ccttctcgac gatcacctcg ttctgctccc
     1801 gggtgaacgt ccggtccatc atgaggatga ggccggcgcg gaccttgggg tactcgaggc
     1861 tcgcgaggtc gaggccgcgc gcggcggcca tgatgatgtg gtcgaggtcg cgctcgcccc
     1921 cgcggttgcg cttcatcggg ttgaagcgaa gctcgagcgt ggtgatgttc tgcttgcggt
     1981 acgcgccgcc gatcgcgccg tggaccgagc gctcgaccgc gagcgggctc gactggatga
     2041 gctccgtcca tttgtagatc gcgtcgagct gcggcagccc ctcgaccccg cgcgggtcga
     2101 ccgagacgag ctccacgaag tcccagtagt ccttcaccgg gagcgcgatg ccctgctcgt
     2161 ggccgagcgt ccacatgacc gccgggtcga ccgacccgcc gaggtgcgtg tgaagctcgg
     2221 cgaggccgcg ccggctgatc ccctcgacgg cggctgcgac ttccgggagg gcctgcgtct
     2281 ccgccatcgg cgcgagtcta cgcgagcgac cccggcgcgc cggcgcggcg tttcggcgct
     2341 aggccgctcg gcgtccgctc gctatgcgcg cgatcgccgc ggtgatgctc gactccttcg
     2401 ccgtcacacg gacgggggag ggcttcgcga cgggcgcggg gtggagctca cggtcggcga
     2461 ggacggaggc cacgtccaca ttgtcgtgga atcgctgcct tccggaagca cccgaaaggg
     2521 tgagggtcag cgtggtgtcc ggcgtaggcc gcgcatcccc ctccgggtag ggttgccgcg
     2581 tgaagatcgg gatcgtcgtg ccgttctcct ggtccttctg gggcggtgtc ccggagcatg
     2641 ccgaccacca ggcgcgcgcg ctccgcgggc tcggccacga cgcgctcatc gtcatgggcc
     2701 acgacccgcc cgggcggctc acgaagatgc tccacccccg gatcggacgg cacgagcgcc
     2761 ccccggagta cgtcatcccg gtcggccgct cggtcatcgt cccggccaac gggtcgctct
     2821 cgaacatcgt cctgaccccg cgttcgatgt tccggatgga gcgcgtgctc gaacgggagc
     2881 gcttcgacgt cgtccacgtc cacgagccga tggcgccgat cctctccacg tacgcgctcg
     2941 tccgggcgag gtgtccgatc gtgaccacct cgcacagctc cggggacacg ctgcgctggt
     3001 acccgatcgc gcacgccgcc tggggcttcc tcgccgagcg gatcgaccac cggatcgcgg
     3061 tctccgagcg ggcgcgcgag gccgccgccc gctacctcgg cgagcccgtt gagatcctgc
     3121 cgaacggggt cgtcatcccg gaggacgcgc atcccgccga ccggcttcgc cacgtcgtct
     3181 tcatcgggcg ccacgagccg cgcaaggggc tgccggtact gctcgaggcg tggccggaga
     3241 tcgctcggcg caccggggcg cggctgaggg tcgtgggcgc cgacccgctc tccgttcgct
     3301 ggctcctccg ccgccagggc gtctccgggg agcacgtgga catcctcggc tcgatctccg
     3361 aggacgcgct cacggaggag ctcctccagg cgaagctgct cgtcgcgccg tccgtcgggc
     3421 gggagagctt cgggatggtg ctcacgcgcg cgttcgggtg cgcgacgccg gtggtcgcct
     3481 cggacatcga gggctatcgc gacgtggccg ggccggagaa cgggctgctc gtcccgcccg
     3541 gcgacgcgcg ggcgctcgcg gaggcggtcg tcgcgctcct cgaggacgag ccgcggcggc
     3601 tcgcgctcgg cgaggcgggc cggcggctcg cccaggaacg gtacggctgg gagcgcatcg
     3661 cgtctcgcct cctcgagatc tacggcggac ttctggacgg cgccggcgag gctcgcgcgg
     3721 cggcatgatg cgcccgcgcc tgccccgctc gcgcccgggg cggctcgcgc ttctcgcgac
     3781 ggcgctcggc gcggcggtcg cgctcgtgct ctggcggctc gacgtcgtcg tcatcacgga
     3841 tgccttctcg ctcgtcacgt gggagtgggt ggcggtcgcc gtggtcctca acctcgcctc
     3901 cgtcctcgtc cggtcggtcg cctggaagat cgtcgtcgac caggcgattc ccccgccgcc
     3961 cccgcggcgg cgcacggtct tcgccgcgtt cagcatcggg ctgctcggca acgcggcgct
     4021 gcccggccgc gccggcgagc tcgcgcgcgt gatcgtgctg aaccgccggc ttccgcaccg
     4081 gccgggaacg tgggcgacgc tcatggggac ggtcttcacg caccggctct tcgacgtcgt
     4141 cgtcgcctcc gggctcgtcg tcttcgtgat ctacgcggcg cgcatcccgt cgtgggctcg
     4201 cccgacgctc gcgatcgcgc tcgggatcgg gatcgggctg ctcgtcgcgg cgctcgtcgt
     4261 cgcccgccgc acgccgcggg gcggcctcga cgggctcggt ccggtccgcc gcctcctcgg
     4321 gctcgcgcgg cgcgggctca ccgtgctcaa gcggccggcg cccgcggcga cggcgcttgc
     4381 gttccaggct gtcggctggg cgacgcagct cctggcggtc tgggcgatgc tctacgcgtt
     4441 cgagatcgac ctgccgatcg aggcggcggc gctcgtgctc gtgatcatga acctcgtgat
     4501 ggtcttcccg ctctggccgg gcaacgtcgg gctcgtccag gcggccgtcg cgctgtccct
     4561 cctcacgtac ggcgtcagct acgggcgtgg cttcgccttc gggatcggcg tgcacgcggt
     4621 cgagctcggg gtcgggatcg cgctcggcct cctcttcctc gcgcgcgagg ggttctcgat
     4681 cgcgatgctg cggcagatgc cggagctccg cgaggtcgag ccggatcagc ccgtcgaacg
     4741 tgtcgcgtga gtcgcggcgc atcgtctgcg cgcccgacag cctgaagggc gtcctctcgg
     4801 cgcgcgaggc ggccgcggcc ctcgcggcgg gagtgcgcga ggccggggga gaggcggacg
     4861 agctcccgct cgcggacggg ggcgagggaa ccgccgaggc gctcgcgctc ggcccgggcg
     4921 agtggcgccg cgcgaccgtg tccgacccgt tggggcgccc gatcgaggcg cgctaccggc
     4981 tctcggtgga cggcgaggcc gtcgtcgagg cggccgaggc gatcggtctc tggcggctcg
     5041 cgccggacga gctcgacccg ctgcgcgcgt cgagccgcgg gctcggagag ctgatccttg
     5101 cggccgtcga ggccggcgcc ctccgtttga cgatcgccct cggcggcacg gcaacggtgg
     5161 acggcggcgc ggggctcctc gaggtcctgc ccgagcttcc ggtcccgacc gtcgcggcgt
     5221 acgacgtgcg gagcccgctg ctcgggcctc gcggcgcggc gcggatgttc gggccgcaga
     5281 agggcgcggg ccctgcggag gtcgaggtcc tcgaggagcg gctcgccgcg agcgcgcgcc
     5341 tcgggccgta cgcggacgtt ccgggcgccg gcgccgcggg cgggctcggc gcggcgctcg
     5401 cctcgctcgg cgcggagctc cggcccggcc tcgagctcgt gctcacggcg actcgcttcc
     5461 gtgagaggat cgcgggggcg gcgctcgtcg tgaccgggga ggggacagtg gatgcctcct
     5521 ccgcggaggg gaaggtgccc gccggggtcg tggacgcggc cgcggaggcc ggcgtcccgg
     5581 tcgtcgtctt cggcggccgc gtcgagccgg ccggcgccga cgcgctgctc gccgcggggg
     5641 cgcgggaggt ggtcgcgctc agcggcgacc ccgggcgggc gcgcgacgat ctcgtcgcgc
     5701 tcggcgcgcg gctcgcgtcc tagatcccgc cgtgccggat cgcttcctcg tcgcggcgga
     5761 cgtcgatcgc gctgtcgagc tccttgccgg tcaccgtcgg gtcggcgatg tcgcccgccg
     5821 gcccctcgga cggctcggcg cgcgcctcga cgcgttcctc ctgtgtgtcc cgcggccgcc
     5881 cgcgcagcca gtcgaacact cccatgagct ccctcctttc ctccagaccg gcttcccgcg
     5941 gccccgagcg gggaaaccgt cgtgtgtagc agcgcgcgcc cggcggcggg agaatgcggc
     6001 cgtgggagcc cacgcttcca gcgacggcga gagcggctgg accgccgaga cgcggcttcg
     6061 gttcctcgcg caggcgaccg acctgctggc ggggtcgctc gactacgagg agacgctcga
     6121 gcgcgtcgcc cagctcgcgg tcccggcgct cgcggactgg tgtgtcgtgg acgaggtgcg
     6181 cgacgacggc gagctcgccc gggtcgccgt cgtcgcggcc gacacgcgga agcaggagct
     6241 gctcgagctc ctgcaggagc gctatccgcc gacctgggac tcgccgcagc cggccgcgca
     6301 cgcggtgcga acccgcgacg ccgtcctgtt cgccgagctg acgccggagg tgatcgcggc
     6361 gaacgcggtc gacgagggac acgcgacgct gctccgcgag ctcgagccgc gcgccgcgat
     6421 cgccgtcccg ctcgtcgcgc acgacgaggt gatcggggcg atgagcttcg cggccgcgga
     6481 gtcgggccgg cgctacggcg aggacgacgt ccggttcgcc gagcagctcg cgcggcgggt
     6541 tgcgctcgcg ctcgaccgcg cccgcctcta ccggagcgag cagcgcgcgc ggctcgaggc
     6601 ggaggcggcc ggcgagcgcc tgcggttcct cgcggagacg accgcgatcc tcggctcctc
     6661 gctcgactac gagacgacgc tcgagcgcct cgcgcgcctc gtcgtcccgg cgctcgcgga
     6721 ctgctgcgtc gtcgacgtgc tggacgagcg gcgccaggtt cggcaggtcg ccgtcgcgca
     6781 cgtcgacccg gagcgggccg agctcgtccg gcggctcgag gagcgctatc cgacgagcga
     6841 ggccgatccg cgaagcctcg tcggccgggc ccttcgcgag gcgacgaccg tcgagctccc
     6901 ggacctagac gtggaggtgg acgagatcgc gcaggacgcc gagcatgccg aggggctccg
     6961 cgagctcgcg ctcgtgtccg ggatgatcgt cccgctgatc gcgcgtggac agacgctcgg
     7021 cgcgatcacg ctcctcacgt cgacgtcgct ccgccgctac gggcccgacg acctgacgct
     7081 cgccgagcag cttgcgagcc gggccgcgat cgcggtcgac aacgcgcggc tctaccggga
     7141 ggccgccgag cgcggccggg ccgcccgcgc cctcgcgacc gtcgcggacg gcgtgctgct
     7201 cctcgaccgc gacggggtcg tacgcctctg gaacccgggc gcggagcgga tcaccggcct
     7261 cgccgccgcc gaggtgctcg gccgcccggc gcgggacgcc atccccgggt ggtcggaggt
     7321 cgagcatgcg ctgccggtcg cgcacgaggg cggcccggcg ccgcgcccgc tcgccgtgcc
     7381 gctcgacatc ggcggccgcg agctctggct ctcgttcgcg ggcgtcggct tcgaggacgg
     7441 caccgtcttc accttccgcg acctcaccga ggagcgccgg ctcgaggagt accggtcgga
     7501 gttcgtcgcc acggcgtcgc acgagctccg cacgccgctc gccgccgtct acggggccgc
     7561 ggtgacgctc cggcgcggcg acatccggct gaccgaggag gagcgggaag tgctcctcgc
     7621 cgtgatcgcc gacgagtccg atcgcctcgc gcgcatcgtg aacgacatcc tctgggcgag
     7681 ccgggtggac tcctccgaca cggacgtccg catcgcgccg ttcgacgcgc gccagaccgc
     7741 ccggcgcgtg atcgagtcgg cccaggcgca ccttcccgaa gggatcgaga tcgcgctcga
     7801 ggcgcccgag ctcctgccgc gcgtcgccgc cgacgagccg aagctcgcgc aggtgctcac
     7861 gaacctcgtg gacaacgcgg tcaagtactc gcccgacgga ggccgcatcg ccgtccgcct
     7921 cgagcccggc cggggccgcc tgcgcctcgt cgtcgaggac gagggcctcg ggatcccggc
     7981 gggcgagcac gagcggatct tcgagaagtt ctaccggctc gacccgaacc agacccgagg
     8041 cgtgggcggc acgggcctcg gtctctacat ctgccgcgcg ctcgtcgagc ggatgggcgg
     8101 tcgcatctcg ctccgatcgg aggagggaga gggctcggcg ttcacggtcg agctcccgct
     8161 cgccgccgac gtgaagatgt aaggattttc ctgcgcgttt ctccgggccg tggagcggga
     8221 agcatgacgc gacacgaccg aagggagccc tgtgtacatc ggaatcggaa cgatccttct
     8281 catcgtcctg atcatcctgc tcatcgcgtt cgtcttctag catcgacacg ctccggccgg
     8341 ccccaacggg ccggccggcc gcatgtccgg gcgcgcgtac gggcgcttcc ctcttcaaga
     8401 tgcgggccag tccccatggc ccggcggttc ggacctcctc acgctgcctg catgggcctc
     8461 gtgagctgga tcctcgtcgg cgtgctcgtc ggcctggccg cgcagcggct ccgaccgcgg
     8521 acgttcccgc tccgagcccc gggcaccgtg ctcgcgggcg tgctcggcgc gtcgttcggt
     8581 gggttcctcg ccgctgcgac gtccgagcgg cgcattgccg agctcgacct cctgaccctc
     8641 gccgcggcgc tcgccgggtc ggtggcggtc gtcgtcctcg tcgcagtggc cgggcgtccg
     8701 cgcgacacgc agcgcgagcg ctcgtgaggc gcgatcgtgg taggacccaa ggacgtgtcg
     8761 ctcttctggc gggtcttcat cgtgaacgcg gcggtgttcg cggtcgccac gctcgtcctc
     8821 gcgctctcgc ccgcgaccgt gagctggccg atcgtcgcgc gagaagggat cgtgctcgcc
     8881 ggcggcctcg ccgcgcttct cgtcgcgaac gtcttcctgc tccggatcgc gctcgggccg
     8941 ctcgagcggc tcacgcgcct catgcgctcg atcgacctcc tgaagcccgt cgggcgggtg
     9001 cccgctgacg gcggcggcga ggtgggcgag gtgatccgaa ccttcaacga catgctcgac
     9061 cggctcgagt ccgagcggcg cgcgagctcg gcccgcgtcc ttgcggggca ggaggaggag
     9121 cgccggcgca tcgcgcgtga gctccacgac gagatcggcc agtcgctgac cgcggtgctc
     9181 ctcagcctga agcgggtcgc cgcacaggcg cccgccgacg tcgccgcgga gctcgaggag
     9241 gtgcaggaga cggcgcggac gagcctcgag gagatccggc ggatcgcccg cgcgctccgc
     9301 cccggagtgc tggacgacct cgggctcgta agcgccctga ccgccctcac gacctccttc
     9361 tccgaggcgt ccggcatcgc cgtcgagcgg cgcttccacg ggccgttccc cgagctcccg
     9421 cgcgacgcgg agctcgccct ctaccgcatc gcgcaggaga gcctgacgaa cgtcgcgcgc
     9481 cacgcggacg cgacctccgt cgtcctcacg ctcgtccgcc gcgacggcgc gatcgtgctc
     9541 gagatcgccg acgacggccg cggcgccggc gcggacggcc acgagggcgg ggggatccgc
     9601 gggatgcgcg agcgcgcgct cgccgtgggc ggcgcgctcg gggtcgagtc gaacgggcac
     9661 ggcgtccgcg tgacgctcga ggttcccgtc gcggaaggag ggcgccgggc gtgacggcgg
     9721 cgacgcggat cctcctcgcg gacgaccaca cgctcgtccg gcgcggcctc cggctcatcc
     9781 tcgacgcgga gccggacctc gaggttgtcg ccgaggccgg cgacggggcg gaggcggtcg
     9841 gccgcgcgct cgcggtcgag gtcgacctcg cgatcctcga cgtcgcaatg ccgcgcatga
     9901 ccggccttca ggcggcgcag cagctcgcga agcgcgcgcc gaaggtgcgc gtgctcatgc
     9961 tctcgatgta cgacaacgag cagtacctct tcgaggcgct caaggcgggc gcctccggat
    10021 acgtgctgaa gtcggtcgcc gaccgcgacc tcgtcgaggc gtgccgcgcg acgatgcgcg
    10081 gcgagccgtt cctctacccc ggcgccgtga ccgcgctcat ccgcgaccac ctggaacgcg
    10141 gccgcgcggg tgaggagatg ccgaacgacc tcctcacccc gcgcgagtcc gagatcctga
    10201 agctcgtcgc cgagggccac tcgtcacgag agatcgcgga gacgctcgtg atcagcgtca
    10261 agaccgtcga gcgccaccgg gcgaacatcc tcgagaagct cgggatgcgc gaccgggtgg
    10321 agctgacgcg ctacgcgatc aggcgcggcc tcgtcgagcc ctagtacgga ggccggtcgt
    10381 agcggtcgtc gcggacgacc gtctcgcgac gccagccgcc gacgccgccc cacgtcgacc
    10441 agaagatcat cgagatcagg aggccgaggg cgccgatgat cacgaggatc aggccgacca
    10501 tgttgatgtc gatgccgctg acctccgcgc tgacggcgag ccagaggatg aggccgatcg
    10561 cgatgaggaa gaggctcacg ccgagaccca ttcgtccctc ctaagggatt ggttacgggg
    10621 gttcttcgct gcggaggggg atacccctcg cgcgcgccgt tcaaccccgg tcagccgcgc
    10681 tgctgggcgg cgcgcaggcg gcgcgagagc gcctggagga gcttccacgc gacctccggc
    10741 tgctcgcgga cgaacggccg gaactcccac ggcgtgagga agaagagatg caggtccttc
    10801 gtggccacga ccgaggcgga gcgcacgtcc tcgtcgatca cggacatctc gccgaagctg
    10861 tcgcccgggc cgaacgtcgc cttctcctcg ccgccgatgc tcacggtcgc ctcgccgtcg
    10921 gcgatcacga agaagctcgt gccggtcgag ccctcctcga ccaccgtctg gcccgccggg
    10981 acgctccgct cctggaagcg gtcggcgagc cgggtcagct cgtcctcgtc cagaccctcg
    11041 aagagagcga cgcggcggag gacgtcgatg ggggcgcggg ccacggcgcc ggattctatc
    11101 cccgggaggg agcgccgcgg aggcggcgaa tcggcgaagg atgacgacgc aggccgcagg
    11161 ccttccggcc gtcgatccgg ccctctcgtg gtccgaggcg gagctccccg agcgggagcg
    11221 gacgaagcac gtgcaccggc tccatcccta cctggggaag ttcgtgcccc agctcgcgga
    11281 ggcgttcctc cgccgctacg cccggccggg gcagctcgtc tgggacccct tcgccgggtc
    11341 ggggacgacg ctcgtcgagg cgaacgcgct cgggctccgc gcggcgggct gcgacctgag
    11401 cgcgttcaac tgtctcctcg cggaggtgaa gacggcgccg tacgaggcgc gcgggctcgt
    11461 cgccgacgtc gcctggctct cggtgcccgg cgcggagccg ccgccggcga tcgcgtcgcc
    11521 gtacctctcg cggtggttcg cgccggcggc gctcgcggag ctcctggcct tccgcgcgcg
    11581 gatcgccggc acggcgtacc ccggcctctg gcgcgtcgtc ctctcgcgcg ccgcgcgctc
    11641 ggcccggctc gcgcggcacg acgatctcga cttcccgcgc gagccggtgc gcggcgagta
    11701 cttctgccac aagcatcgcc gcgtctgccg cccggtcggg gaggcggaca agttcctgcg
    11761 ccgctacgtc cgcgacgccg cctgccgcgt gcgcgagttc ggggacagtc gcacggacgc
    11821 ggccgtgacc gtgctcgagg gcgactcgcg cgaggtcgac ccgcccgggc cggtggatct
    11881 cgtcttcacg tccccgccgt atccggggct catcgactac cacgagcagc acgcgtacgc
    11941 cttcgagctg ctcggcctcg agcggcgcga tcccgacgag atcgggcgcg gcgtccgcgg
    12001 ctattgcgag gacgtcgccg ccgtgctccg gcgcgcgcgc cgcgcgctgc ggcgcgacgg
    12061 ccgcgtcgtc gtcgtcgtga acgactcccg cggcctctac gacgggatcc tcgccgccgc
    12121 cggcctccgg ctcgaggagc ggatcctccg cgaggtgaac cgccgcaccg gccggcgcgc
    12181 gggggcgtac tacgaggaga tcctcgtcgc ccgcccgtag atcccgtccc ggacgagcgc
    12241 gcacgagccg gggatccggg tctcctcgac ctcgccgacg tcgaagccgg cccgcgcgac
    12301 cgccgccttc gtgtcgcggt tcaggtggca gttgccgaag aaccgacgct ggagcgggtt
    12361 cagccggtcc tgccagaccg cgacgcgccc gtgcccgcgc acgtgctcga gcaggttgag
    12421 cgtcccgccg ggcctgagga cgcggtggat ctccgcgagc gcctgcgcct ggtcggtcac
    12481 cgagcagagg acgaagaccg agacgaccgc gtcgaagctc tcgtccggga acgggagcga
    12541 ctcggcctcg gcgtcgacga cctcgaccgg agcgggcgcg ccggcgatct tgcccgggat
    12601 gcgcgcggcc atgctcctgt cgggctcgag ggcgacgacg cggctcgcct tgaggtagtg
    12661 cttgaggctg aacccggtgc cggctccgat ctcgagcacg tcgccctcga gccgcgcgac
    12721 gagcgagcgg cggcggtgcc cgatctcgct ccgctcctcg aagcggccga gcaggtcgta
    12781 gagccggccg ttcactccgg gcgcgcgtcg cccgcgcggc ggccgagctc ctcgatcgcg
    12841 ccctgcgcgg cgtcgagctt cgcgaggcag atccggtgga gctcctcgcc gcgctgccag
    12901 agcgcgagcg cctcctcgag gcccgcgcgt ccgctctcga gctcggcgac gatcttctcg
    12961 agctcggcgc gcgcctcctc gaacgtcggc tccgccttgg cctgccccgg ctcgctcacc
    13021 gggtctcctc gacgaccgcg ccgaagccgc cctccgcgag ccgcacgtcg acgcgctcgc
    13081 ccggcgcgag cgccgagccg gagcggacga ccgcgtcccc cgagcgcacg atcgcgtagc
    13141 cgcggccgag cgtcgcctgc ggagagagcg cgcgcaggct gccggcgagg ccgtccagct
    13201 cggcgcgctt gcgcgcgacg agcgcggcgg gcgcgcgggc gaggcgggcg gcgtcggcgt
    13261 cgagccgttc ccgggcccgg gcgaccgtac gtgcgccctc ccggccgagc gcggcgtgga
    13321 gggcgctcac gcgctcgcgc gagcgggtcg ccgtgcgggc cgtctcgcgc gcgagcgagg
    13381 ccctcagcga cgcgagccgc tcgcgaagct cgccctcgtc cgggacgacg agccgcgccg
    13441 ccgccgtcgg ggtcgacgcg cgcgcgtcgg cggccaggtc gcagagcggc gtgtcctgct
    13501 cgtggccgac cgcggagacc accggcacgg ggcaggcggc aacggcccgc acgacccgct
    13561 cgtcgctgaa ggggaggaga tcctcgaagc tcccgccgcc gcgcgagacg acgaggacgt
    13621 cgatctccgg ctcggccgcg agggcggcga gcccggccgc gatcgcgcgc ggcgcgcccg
    13681 gcccctgcac ggcggtctcc gcgacgagca ctcgcgcggc ggggaaacgc gcctgcacgg
    13741 cggtcacgaa gtcccgcttc gcggccgcct ccgcgccggt caggagcccg atccggcggg
    13801 ggaacaccgg gagcggccgc gagcgggcga agagcccctc gtcggcgagc ctgcgcttca
    13861 ggcgctcgat ccccgcgagg agcgcgccga gcccctgcgc ctcgagcgag agagcccgca
    13921 gccggaacgt gccgcgcgcg gtgaagagct ccggccggcc gaagacgtgc acggtcgccc
    13981 cgtccgcgag gtcgagcccg agggcgtcga aggtcccgcg cggcatggag accggcaggc
    14041 acgcgccgtc ggccgggtcc ttgagcgtga agaagacgct ctgccagccc gcctggcggc
    14101 ggagctcagt cacctccccc tcgacccaga cggtcggcag ccgttgcagc cacgacgcga
    14161 ccccacggtt gaacgcggag acgggataga ccttgcggtc gccgtagagc acgggctccg
    14221 gccgcacgcg gccagcctac ccgcgcccgc gccggtcgcc gagccgccgc ctagagcagg
    14281 atctcggccg cgagctcgtg cgcgcagccg cgctcggtca cgagcgtcac tgcctcgtgc
    14341 aggtcggcgt gcgagcgtgc gagccgctcg gcgaggggga gcgggtagcc ggcctcggcg
    14401 agcacgtgca ggcgccagct ctcgaccttc gcccgctcgt cgatgcccgt ctcgactgtg
    14461 gtggtctcgt ccatgacgtc ttccatgtcg ccggtggttc gtccttatcc cgacgaaccc
    14521 ttccggaaaa agttgtcgac aaccggccgt gcgtgtcgcg ggatccgcgg ggcggatgga
    14581 cgtctagagg tcgcggagcc gtgtcgccac ctgggcctcg atccgttcct cgagctccgc
    14641 gatccggcgt tcggcgaccg agagggcgtc ctcgagccgc tcgttcatcg cgctcatccg
    14701 cgccgccgag acgtcgtact ggctctcggc cgccttggcg ctgtcggtca ggcgccgctc
    14761 gaggcgcgcg gcggtgttct gggcggcgtc ggcgatccgg tcggcgatgt ccttctccgc
    14821 ctggcgggcg aactgctcga ccgacttctc gagctcgttc gacaggcgct gcgcggactt
    14881 ctcgcgcgcc ttgcggatct gcacgtcgaa gcgccgctcg gactcctcgg agaggcgcga
    14941 cgaggaccgg tcgagcaggc gctcgaggtt ctcgcgctgg cggcgctcga cctcggcgaa
    15001 cgtgctcgtg atccgcgcgg ccgcctccgc ctcttcccgc tcgatgtggc cgcggagatc
    15061 cttctcctgc tcgcggacgc gcttggtcag ctcggcgagc gcccggcggc gctcgtccgt
    15121 gtgcgcctcc atctcggcgc ggccctcctc gaggatctcc cggccgacct tctgcagctc
    15181 cgtgcggagg cgcgcgagcg cagtctgctg ctcctcgctg atcgtgcgga ggaactccga
    15241 gtcggccgcg agccgcgcct cgtacgcctt gatcgcgtcg ccctggcgct gcgcgagctc
    15301 gttcaggcgc gtctcgagcg cgcgctggcc gcggtcgagg tcggcggccc aggcggcgag
    15361 gcgctgctcc aggcgctcct gcacctgctc gatcgacttc gcgagctcgg cgcgcgcgcc
    15421 gtccgcctgg cgcgcgacga ggtccagccg ctcgaccgcg agcttgcgct cctgcttcgc
    15481 gagctcgtgg ttcgcgttcg ctcgctcgcg ggcgagcgtg tgctcgagct cctccgcgcg
    15541 gatccgggcg tgctcctcga gctggtcctc gatccgctgc cgtccggcgg cgaggagccg
    15601 gtcctcctcg cggagccgct cgagcccgcg cgcgaggacg aagagccccg cttccgcgac
    15661 ggccgcggcg gcgagcccgc ctgccgcgag gacggtcgcc gagccgtcga cgaacgagcc
    15721 ctcgacgttc agcccggcga ccgcgatcgc gatcccggtc gcgacgatga gcccgcccgt
    15781 gatccagcgc agcgcacgcg agccgatgcg gcctgcgagc agcgctccgg cggagccgag
    15841 cacgacgagg aaccccacgg cgaagacggc ctcgaagacg gtctccatcc gcagcatcct
    15901 agaaccagag cgcgaaggga gcgtcgctcc cgtgcgggcc gacgcggtgg tcgcggagcc
    15961 actggagcgc gacccggccg acgtccgcgg cgagggtgtc cgaggcgtcg cccgcgattc
    16021 cgggcgtcca cgtgaggacg acgacggccg cggtcccggc gcgcgtgtag acgagcccgg
    16081 cgtcgtggcg cgcgttcgtc acccagccgc ccttgtgggg gacgaggacg ccggtccccg
    16141 cgtggcggtc tagcttcccc cggtcggccg agtgggcgag gatccagagg aggtagcgcg
    16201 cgtccgccgg cgcgaacgag ccggtcagct cgagcagcgg gccctcgccg cgggcggcct
    16261 ggtggagcca gcggtagagg cgcgcgaggt cc
//
LOCUS       DASTFC010000299         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_254826,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000299 DASTFC010000000
VERSION     DASTFC010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254826"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1525)
                     /locus_tag="VFO88_08920"
     CDS             complement(116..1525)
                     /locus_tag="VFO88_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET9545688.1"
                     /translation="MDRAELEPALALVAERARAYLAGLDDAPLHAPNVEEAAWSFDGS
                     LPEEGSGAEATLARLLDEGLDGTIASAGPRNFHFVTGGVTPAALGADWLASLLDQNSF
                     AWIESPLGAQLERVALRWLRELFDLPAEWGGVLTTGATMANFVSLAAARRWWGERHDR
                     DVDLDGFAGLPAVPVFSSGYVHATAIKALGMLGIGRASVRTFGADDAGRLDRDALREA
                     LEALDGAPAILIGNAGEVNAGDFDPLDELADLADAHGAWLHVDGAFGLFARVSPETAP
                     LAAGAERADSVIADGHKWLNVPYDCGFAFVRDGSRLLPVFNASAAYLPQDDPDRPNYG
                     LLGPESSRRGRSFAVWATLAAYGRAGYRELVERNLGHARLAARLVDEAPDLELLAPAP
                     LNIVCFRYRPEDVPDDALDDLNARLGETLLADGRVYAGTTRYRGHVAFRPAFVNWRTR
                     DQDVELLVHTIRELGASLR"
     gene            complement(1597..1788)
                     /locus_tag="VFO88_08925"
     CDS             complement(1597..1788)
                     /locus_tag="VFO88_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545689.1"
                     /translation="MQTSVRARLLGIPLLRLDATVVLMPSAITTGASPLAPRDGLAEA
                     VRSINDGAELLAQVRNGRR"
     gene            complement(2078..>2361)
                     /locus_tag="VFO88_08930"
     CDS             complement(2078..>2361)
                     /locus_tag="VFO88_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HET9545690.1"
                     /translation="QLRNEAEQRATDAEARLKAVEDLHERAAAAAKRTARGLDRETVD
                     GGLDAYKKPELVELAASIGIENRTTMTKSELVDAITKAARAGTRQGASR"
BASE COUNT          293 a          851 c          895 g          322 t
ORIGIN      
        1 tcggcgggag agcgtccgtg cgaaggcagg cggcgccgca tgggcttaga gaggtaacgg
       61 gccgcgcccg cgcgcgccat gggtggcgtc atgtatctgg gaggcgttcg cacggtcatc
      121 ggaggctcgc tcccagctcg cggatcgtgt ggacgagcag ctccacgtcc tggtcgcgcg
      181 tgcgccagtt tacgaaggcc gggcggaacg cgacgtgccc tcggtaccgg gtcgtgccgg
      241 cgtagacgcg gccgtcggcg agcagcgtct ctccgagccg ggcgttcagg tcgtcgagcg
      301 cgtcgtccgg gacgtcctcg gggcggtagc ggaagcagac gatgttgagc ggggccggag
      361 cgagcagctc gaggtcgggc gcctcgtcca cgagccgggc cgcgaggcgg gcatgaccga
      421 ggttccgctc gacgagctcg cggtacccgg ctcgcccgta ggcggcgagc gtcgcccaga
      481 cggcgaagga ccggccgcgc cgcgagctct ccggcccgag gagcccgtag ttcggccggt
      541 cggggtcgtc ctgcgggagg tacgcggcgc tcgcgttgaa gaccggcagg agccgggacc
      601 cgtcgcggac gaaggcgaag ccgcagtcgt acgggacgtt cagccacttg tggccgtccg
      661 cgatcaccga gtcggcgcgc tcggcgcccg cggcgagcgg cgcggtctcc gggctcacgc
      721 gcgcgaagag gccgaacgcg ccgtccacgt ggagccatgc gccgtgcgcg tccgcgaggt
      781 cggcgagctc gtcgagcggg tcgaagtcgc ccgcgttcac ctcgcccgcg tttccgatca
      841 ggatcgccgg agccccgtcg agcgcctcga gcgcctcgcg gagggcgtcg cggtcgagcc
      901 ggccggcgtc gtcggcgccg aacgtccgga cgctcgcgcg gccgatgccg agcatcccga
      961 gcgccttgat cgcggtcgca tggacgtagc cgctcgagaa caccggcacg gccggcaggc
     1021 cggcgaagcc gtcgaggtcg acgtcccggt cgtggcgctc gccccaccag cggcgggcgg
     1081 cggcgaggga gacgaagttc gccatcgtcg ctccggtcgt gaggacgccg ccccactccg
     1141 cgggcaggtc gaagagctcg cggagccagc ggagcgccac ccgctcgagc tgcgcgccga
     1201 gcggcgactc gatccacgcg aacgagttct ggtcgaggag cgacgcgagc cagtcggcgc
     1261 cgagcgcggc gggcgtcacg cctccggtta cgaagtggaa gttccgcggg ccggccgacg
     1321 cgatcgtgcc gtcgagcccc tcgtcgagga gccgcgcgag cgtcgcctcg gctcccgagc
     1381 cctcctccgg gagcgagccg tcgaagctcc aggccgcctc ctcgacgttc ggcgcgtgga
     1441 gcggagcgtc gtcgagcccg gcgaggtagg cgcgtgcccg ctcggccacg agcgcgaggg
     1501 ccggttcgag ctcggcgcgg tccacgccgg gagcctacga cgcggccgcc cgccgggcac
     1561 gacgaaccgc cggggaagat gaccggccgg gcggcctcag cgccggccgt tgcgcacctg
     1621 agcgagcagc tctgctccgt cgttgatgct gcgcaccgcc tcggccagcc cgtcccgcgg
     1681 ggcgagcggg gaggctcccg tcgtgatcgc gctcggcatg agcacgaccg ttgcgtccag
     1741 ccgcaggagc gggatgccca gcaggcgcgc acggaccgag gtctgcacga gcgcgccctc
     1801 ggagaggcgc ctgctgggat cgacggcggc ggtcagcgcg accggagcgt gcgtttcgat
     1861 gccccgagcg gcccgccgcc gcctcatgag tcgcttccct ccgttcggcg gcggatcgag
     1921 gagatggcgg cactccccgc cgtgagggcg gctaccccgc cggccaccag ggtcgccttc
     1981 aggagcttgc tccggctcat gccgccgccc agggcggcaa ccgagcctcc gaccgtgtcg
     2041 agaagccgat ccggcctgct cttcgtgcgg agcggtctca tcggctcgct ccttgtctcg
     2101 tcccggcacg agcggccttc gtgatggcgt cgacgagctc gctcttcgtc atggtcgtcc
     2161 gattctcgat tccgatactg gccgcgagct cgacgagctc cggcttcttg taggcgtcga
     2221 gaccgccgtc caccgtctcc cggtcgagcc cgcgcgcggt gcgcttcgcg gctgccgccg
     2281 cccgttcgtg caggtcctcg accgccttca ggcgagcctc ggcgtcggtc gctctctgct
     2341 cggcctcgtt ccggagctgc t
//
LOCUS       DASTFC010000300         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_535093,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000300 DASTFC010000000
VERSION     DASTFC010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535093"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFO88_08935"
     CDS             complement(<1..706)
                     /locus_tag="VFO88_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET9545691.1"
                     /translation="MTSPLARPRGAFRATAAAKVADPHAQQALDEATGRLYGHRLAAW
                     GELEGVEALRERARQIRTGTLEHLEAHVDAFARAVEARGGRVRRAASAAEAREAVVEI
                     CRRAGARRAAKSKSMATEEIGLNPALEAAGIEVVETDLGEYILQLGGEHPVHIVAPAI
                     EKTAAEVAELFSRVEGRPVSDDLAELTATARRRLREVFLTADVGITGANFGVAETGSI
                     CLVSNEGNARLVSSLPR"
     gene            complement(703..1443)
                     /locus_tag="VFO88_08940"
     CDS             complement(703..1443)
                     /locus_tag="VFO88_08940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET9545692.1"
                     /translation="MRILSVIHGTNARAGVFGEEALAEGHELEEKSFALGDPPDDVER
                     YDGVLVFGGSMNVHELDGHPWIPDEARYVERALERGVPVFGVCLGSQLLGAVAGAEVS
                     RAPQPEIGWFDVETTSDAPFDPLLRDLPERFTAYQWHSYQCELPVGATALAENPVCLQ
                     AYRLGDAPAWGTQFHAEVTREICESWIAKYDTDPDAVAQGFDPEVERARVADEIGRWN
                     ELGRRLMRGFLSVAAERAGAEPAARASA"
     gene            complement(1454..2311)
                     /locus_tag="VFO88_08945"
     CDS             complement(1454..2311)
                     /locus_tag="VFO88_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9545693.1"
                     /translation="MTLLALAVLGRGVVPPEEPVLHADDEALLRGRAAFETTRVYGGR
                     PFRLDDHLARLAGSAARIGLPAVDEDACHALARDALGAAGAPDAVLRLYWTAGREGTN
                     APSGLALVSTVPPELEERRSRGLRVVALTLGFEADLRAVAPWMLGGVKSTSYAVNMAA
                     EAEARRRGADDAVFLARDDVVLEGPVTNIWWRLERVLYTPALELGILAGVTRATVVEE
                     AAALGYEVREGVFPLAHLAAAEEAFTSSSVREVMPVVELDGHPVGDGAPGPAARELQA
                     ALRARAARG"
     gene            complement(2308..>2677)
                     /locus_tag="VFO88_08950"
     CDS             complement(2308..>2677)
                     /locus_tag="VFO88_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET9545694.1"
                     /translation="LVSRVEGLVREDASLADMLRATFPGGSVTGAPKIAALDLIAALE
                     PVGRGASMGALGRIRPNGDLDLALTIRTFAVADGRIHLWVGGGIVWDSDPDAEVEESW
                     VKARPLLAAVGGRVPGPVAA"
BASE COUNT          338 a         1061 c          966 g          312 t
ORIGIN      
        1 cgcgggggag ggacgagacg agccgcgcgt tcccctcgtt cgagacgagg cagatggagc
       61 cggtttccgc cacgccgaag tttgcgcccg tgatcccgac gtccgcggtc aggaagacct
      121 cccgcagccg ccggcgcgcc gtcgcggtca gctccgcgag gtcgtcggaa accggtcggc
      181 cttccacgcg cgagaacagc tcggcgacct cggcggcggt cttctcgatc gcgggggcga
      241 cgatgtgcac cgggtgctcg ccgccgagct ggaggatgta ctcgccgagg tcggtctcga
      301 cgacctcgat tcccgccgcc tcgagcgcgg ggttcaggcc gatctcctcg gtcgccatcg
      361 acttcgactt cgcggcccgc cgcgcgccgg cccgccggca gatctcgacg accgcctcgc
      421 gggcctccgc ggcgctcgcc gcgcgacgga cgcgtccacc gcgggcctcg acggcgcggg
      481 cgaaggcgtc gacgtgcgcc tcgaggtgct cgagcgttcc cgtgcggatc tgccgcgcgc
      541 gctcgcggag cgcctcgacg ccctcgagct cgccccaggc cgccagccgg tgcccgtaga
      601 gccgcccggt cgcctcgtcg agcgcctgct gggcgtgcgg gtccgcgacc ttcgccgcgg
      661 ccgtcgcacg gaacgctccg cggggccgcg cgagggggga ggtcacgcgc tcgcgcgggc
      721 ggccggctct gcgccggcgc gctccgcggc gacggagagg aagccgcgca tgagccgccg
      781 cccgagctcg ttccagcggc cgatctcgtc cgccacgcgg gcgcgctcga cctcgggatc
      841 gaagccctgc gcgaccgcgt ccggatccgt gtcgtacttc gcgatccagc tctcgcagat
      901 ctcccgcgtc acctcggcgt ggaactgcgt cccccacgcc ggcgcgtcgc ccaggcggta
      961 cgcctggagg cagacggggt tctccgcgag cgccgtcgcg cccacgggca gctcgcactg
     1021 gtagctgtgc cactggtagg ccgtgaaccg ctcgggcagg tcgcggagca gcgggtcgaa
     1081 cggcgcgtcc gaggtcgtct cgacgtcgaa ccagccgatc tccggctgcg gcgcgcgcga
     1141 gacctccgcg ccggcgaccg cgccgagcag ctggctcccg aggcagacgc cgaagacggg
     1201 gacgccccgc tcgagcgccc gctcgacgta gcgcgcctcg tcggggatcc agggatgccc
     1261 gtcgagctcg tggacgttca tcgagccgcc gaagacgagg acgccgtcgt agcgctcgac
     1321 gtcgtcgggc gggtcgccga gggcgaagct cttctcctcg agctcgtgcc cctcggcgag
     1381 cgcctcctcg ccgaagacgc cggcccgcgc gttcgtgccg tggatgaccg agagaatccg
     1441 catgcgcaag agcttagccc cgcgctgcgc gggcgcggag cgccgcctga agctcgcgcg
     1501 ccgccggccc cggagcgccg tcgccgaccg ggtgcccgtc gagctcgacg accggcatca
     1561 cctcgcgcac cgacgacgac gtgaacgcct cctccgccgc cgcgagatgg gcgagcggga
     1621 agacgccctc gcgcacctcg tagccgagcg ccgccgcctc ctcgacgacg gtcgcgcgcg
     1681 tcacgccggc caggatcccg agctcgagcg cgggcgtgta cagcacgcgt tcgagccgcc
     1741 accagatgtt cgtgaccggc ccctcgagga cgacgtcgtc ccgcgcgaga aacaccgcgt
     1801 cgtccgctcc gcgccgcctg gcttcggcct ccgcggccat gttcacggcg tagctcgtgg
     1861 acttgacccc gccgagcatc cagggtgcga cggcgcgcag gtcggcctcg aagccgagcg
     1921 tcagcgccac gacgcgcagc ccgcgagagc ggcgctcctc gagctcgggc ggaaccgtgg
     1981 agacgagcgc gagcccgctc ggcgcgttcg tcccttcgcg gcccgccgtc cagtagagac
     2041 gcagcacggc gtccggcgcc ccggccgcgc cgagcgcgtc gcgggcgagc gcgtgacagg
     2101 cgtcctcgtc gaccgccgga agcccgatcc gcgccgccga ccccgcgagc cgcgcgaggt
     2161 ggtcgtccag ccggaacggc cggccgccgt acacgcgcgt cgtctcgaac gccgcgcggc
     2221 cgcggagcag cgcctcgtcg tcggcgtgca gcacgggctc ctcgggcggg acgacgccgc
     2281 gcccgagcac cgcgagcgcg agcagggtca cgccgccacc ggccccggca cgcgcccgcc
     2341 caccgccgcg agaagcggcc gcgccttcac ccaggactcc tccacctcgg cgtcgggatc
     2401 cgagtcccag acgatcccgc cgccgaccca gaggtggatc cgcccgtccg cgaccgcgaa
     2461 ggtgcggatc gtgagcgcga ggtcgaggtc gccgttcggg cggatgcgcc cgagcgcccc
     2521 catcgacgcg ccccgcccga ccggctcgag cgccgcgatc aggtcgagcg ccgcgatctt
     2581 cggcgcgccc gtcacggagc cgcccgggaa cgtcgcccgc agcatgtcgg cgagcgacgc
     2641 gtcctcgcgc accagcccct cgacccgcga gacgagg
//
LOCUS       DASTFC010000301         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1457889,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000301 DASTFC010000000
VERSION     DASTFC010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457889"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFO88_08955"
     CDS             complement(<1..833)
                     /locus_tag="VFO88_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9545695.1"
                     /translation="MLDVRRMRVLLEVANRGSISAAAQALSFTPSAVSQQLATLERET
                     GVALVERGPRSVRLTEAGRALAGHAEDVLARLQAAETDLQALAGLRRGNVRVASFPTA
                     YATIMPGAIASFRARHPGVELHLTEADPRAGLARVKAGELDVALVYEYDFVPLPPQEG
                     VELVHLLDDPVHVLLPRAHPAARRRAVRLADLADEPWITSTGRSSCHAFVRRACNASG
                     FDPEVTMESDDHGVWQGLVAAGVGVALVSELSLPVLDPAVVLKPVAGRPLKRGIFAAH
                     RV"
     gene            904..>3803
                     /locus_tag="VFO88_08960"
     CDS             904..>3803
                     /locus_tag="VFO88_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545696.1"
                     /translation="MSAVRRLLFPLRLARARLGRRLGRVALVGLGLAAAAATLAAVLA
                     GGLVAEDRSVGRAIEAIPEPERAVRVAAFGVPGERDPARAELDRAARSALASVAEGEQ
                     VATLLFRTGSIGGRLVDLGAVDGLAPWVRLESGRLPQPCRPERCEVVRIRGGGPAPEP
                     GGVRLVEVGRATLASETLFGRFVEPGAYHQPATPPLLLAEGVETLAAAPSLASRYRTS
                     GWVLPLEAGSVRAWETRALEERVVQARVGLTDVHGDFEIEAPVEAIAAAGETAQAAGR
                     RLLVIGGQAAALLVAFALLAAAALRRDVEASRRRLTWFGARRSQLELGTAAEVGAVAL
                     AGTAVGWALGVGIAVAIAREAEVPAGGILRQSVLSGGGLAAAAGLALGSALVLFLAVR
                     TRPLRLAGLTVTPLDVAALGALAVVAVVLLRGSADADALAREGGTGALLLLLPGLVAF
                     VAAVAFARVLGPALRALERAARGGSVPLRLAALSLSRRPGPAAIAIGFLVVSLGLALF
                     AETYRSTLARGQSDQAAFAVPADAVVREDLRQLVAPLEAAPLPEYERATAGGTATPVL
                     RVQGSVPRLGLEGGVTVLGLPAAHVAEVGGWRDDFADASRAELARAVEPERPAALAGI
                     EMPADARELRLDAAVTGAPVLLSASLQTPAGGFTSVELGRLVEGRPAVLRAAVPQEAR
                     GGRLVALTFSPPIRIEEPGASAGRAVRGVLTTEGIGFAAADGSVETRPFEGWVPTNTT
                     VSAVVQTGPTEFHYALTNQVVSRFRPRQASDEGPIPVLATPGLAAAAGPGGSLALEAA
                     RIDLRVRVVGTVERFPGIEGEGLVADRDLVTTAMNAVTPGAAEPNELWLTSPDPDALG
                     AALAAPPLDVLAVTERAEVERALRADPLARAALLTLGAAALVALGLALVGLVLGVVAD
                     LRDERGELFDLEAQGAAPADLRRQVRLRAGIVAAVGVLGGALTGAVLSLLVV"
BASE COUNT          348 a         1446 c         1529 g          480 t
ORIGIN      
        1 acgcggtgcg cggcgaagat cccgcgcttg agcggccggc cggcgaccgg cttcaggacg
       61 acggcggggt cgagcacggg gagcgagagc tccgagacga gcgcgacgcc gacgccggcc
      121 gcgacgagcc cctgccagac gccgtggtcg tccgactcca tcgtcacctc cgggtcgaac
      181 ccggacgcgt tgcaggcccg gcggacgaac gcgtggcagg acgagcgccc ggtcgacgtg
      241 atccacggct cgtccgcgag gtcggcgagc cggacggcgc gccgccgcgc ggcgggatgg
      301 gcgcgcggca ggaggacgtg gacgggatcg tcgagcaggt ggacgagctc cacgccctcc
      361 tgcggcggga gcgggacgaa gtcgtactcg tagacgaggg cgacgtcgag ctcgcccgcc
      421 ttcacgcgcg cgaggccggc gcgggggtcg gcctccgtca ggtggagctc gacgccggga
      481 tggcgcgcgc ggaagctcgc gatggcgccc ggcatgatcg tcgcgtacgc ggtcgggaac
      541 gacgcgacgc ggacgtttcc acggcgcagg ccggcgagcg cctggaggtc ggtctcggcg
      601 gcctgaagcc gcgcgagcac gtcctccgcg tggccggcga gggcgcgacc ggcttccgtc
      661 aggcggacgc tccgcggccc tcgctcgacg agcgcgacgc cggtctcgcg ctcgagcgtc
      721 gcgagctgct gcgagacggc ggagggggtg aaggagagcg cctgcgcggc cgcgctgatc
      781 gagccgcggt tcgcgacctc gagcagcacg cgcatccggc ggacgtcgag catcaggagc
      841 gcttaacctt accggaagcg atttcacttg tctttacgct ggcccgctgg cagcgtttcg
      901 cctgtgagcg cggtccggcg tctgctcttt cccctgcggc tcgcccgcgc gcgcctcggg
      961 cggcggctgg ggcgcgtcgc gctcgtcggg ctcgggctcg ccgcggcggc cgcgacgctc
     1021 gccgcggtgc tggcgggcgg gctcgtcgcc gaggaccgga gcgtcgggcg ggcgatcgag
     1081 gcgatcccgg agccggagcg cgccgtccgc gtggccgcct tcggcgtgcc gggcgagcgc
     1141 gaccccgcac gcgccgagct cgaccgggcc gcccggtcgg cgctcgcgtc cgtcgcggag
     1201 ggcgagcagg ttgcgacgct cctctttcgc accggctcga tcggcggacg gctcgtggat
     1261 ctcggcgcgg tcgacgggct cgcgccctgg gtgcggctcg agtcgggccg cctgccgcag
     1321 ccgtgccgcc cggagcgctg cgaggtcgtc cgcatccgcg gcggggggcc cgcgcccgag
     1381 ccgggcggcg tgcggctcgt cgaggtcggc cgggcgacgc tcgcctcgga gacgctcttc
     1441 ggccgcttcg tcgagccggg cgcgtaccac cagccggcga cgccgccgct cctcctcgcc
     1501 gagggcgtcg agacgctcgc cgccgcgccg tcgctcgcat cgcgctaccg gacgtcgggc
     1561 tgggtgctcc cgctcgaggc ggggagcgtc cgggcgtggg agacgcgcgc gctcgaggag
     1621 cgcgtcgtgc aggcgagggt cgggctcacc gacgtgcacg gcgacttcga gatcgaggcg
     1681 cccgtcgagg cgatcgcggc cgccggcgag accgcgcagg cggccggccg gcggctgctc
     1741 gtgatcgggg gccaggcggc ggcgctcctc gtcgcctttg cgctcctcgc cgccgcggcg
     1801 ctccggcggg acgtcgaggc ctcgaggcgg cggctcacgt ggttcggcgc gcgccgctcg
     1861 cagctcgagc tcgggacggc ggcggaggtc ggcgccgtcg cgctcgcggg cacggccgtg
     1921 ggctgggcgc tcggcgtcgg catcgcggtc gcgatcgcgc gcgaggcgga ggtcccggcc
     1981 ggcgggatcc tgcgccagtc ggtgctctcg gggggcgggc tcgcggccgc tgcggggctc
     2041 gccctcggct ccgcgctcgt cctcttcctc gccgtacgga cgcggcccct ccggctcgcg
     2101 ggcctcacgg tgacgccgct cgacgtcgcc gcgctcggcg cgctcgccgt cgtcgcggtc
     2161 gtcctcctcc gcggctcggc ggacgccgac gcgcttgcgc gggagggcgg aaccggtgcg
     2221 ctcctcctgc tccttccggg cctcgtcgcg ttcgtcgccg cggtcgcgtt cgcgcgcgtc
     2281 ctcgggcccg cgctccgcgc gctcgagcgg gcggcccgcg gcgggtccgt ccccctccgc
     2341 ctcgcggcgc tctcgctctc gcgccggccg ggccccgccg cgatcgcgat cggcttcctc
     2401 gtcgtcagcc tcgggctcgc gctcttcgcg gagacgtacc gctcgacgct cgcccgcggc
     2461 cagtccgacc aggcggcgtt cgcggtgccc gccgacgcgg tcgtgcgcga ggacctgcgg
     2521 cagctcgtcg cgccgctcga ggcggcgccg cttcccgagt acgagcgggc gaccgcgggc
     2581 ggcacggcga cgccggtgct gcgggtgcag ggctcggtgc cgcggctcgg cctcgagggt
     2641 ggcgtgaccg tcctcgggct tcccgccgcg cacgtcgcgg aggtcggcgg gtggcgcgac
     2701 gacttcgcgg acgcctcgcg ggccgagctc gcgcgcgcgg tcgagcctga gcggcccgcg
     2761 gcgctcgcag ggatcgagat gcccgctgac gcccgcgagc tgcggctcga cgccgccgtg
     2821 acgggagcgc ccgtgcttct ttcggcgagc ctccagacac cggccggcgg cttcacgagc
     2881 gtcgagctcg gccggctcgt cgagggccgt ccggccgttc tccgcgcggc cgtgccgcag
     2941 gaggcgcggg gcggccggct cgtcgcgctg accttctcgc ccccgatccg gatcgaggag
     3001 ccgggcgcgt cggcgggccg ggcggtgcgc ggcgtcctca cgacggaggg gatcggcttc
     3061 gcggcggccg acggctcggt cgagacgcgg ccgttcgagg gctgggtgcc gacgaacacg
     3121 accgtctcgg cggtcgtcca gaccgggccg acggagttcc actacgcgct cacgaaccag
     3181 gtcgtctcgc gcttccggcc gcggcaggcg agcgacgagg ggccgatccc ggtgctcgcg
     3241 acgccgggcc tcgccgcggc cgcggggccg ggcggctcgc tcgcgctcga ggccgccagg
     3301 atcgacctgc gcgtccgcgt cgtcgggacg gtcgagcgct tccccgggat cgagggggag
     3361 gggctcgtcg ccgaccgcga cctggtcacg acggcgatga acgccgtgac gcccggcgcg
     3421 gcggagccga acgagctctg gctcacctcg cccgacccgg acgcgctcgg cgccgcgctc
     3481 gccgccccgc cgctcgacgt gctcgccgtg accgagcggg ccgaggtcga gcgcgcgctc
     3541 cgggccgacc cgctcgcgcg cgcggcgctc ctcaccctcg gcgcggcggc gctcgtcgcg
     3601 ctcggcctcg cgctcgtcgg gctcgtcctc ggcgtcgtcg ccgacctcag ggacgagcgc
     3661 ggcgagctct tcgacctcga ggcgcagggc gcggcgcccg ccgacctgcg ccgccaggtg
     3721 cgcctgcgcg ccgggatcgt ggccgcggtc ggcgtcctcg gcggcgcgct caccggcgcc
     3781 gtgctcagcc tgctcgtcgt cga
//
LOCUS       DASTFC010000302         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1540510,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000302 DASTFC010000000
VERSION     DASTFC010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540510"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFO88_08965"
     CDS             complement(<1..392)
                     /locus_tag="VFO88_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545697.1"
                     /translation="MARIRPELPDGPLVSFGLCGALVDGLPPGTIVTARSVVDEAGEA
                     LWEGPPRAVAGARAVVLCAAGRIVDAAGERTALAERSGAEVVDLESGRLAATGRLAGV
                     VRVVSDTPAVPLGPLAGAVTSAGETDALA"
     gene            complement(455..1453)
                     /gene="hpnH"
                     /locus_tag="VFO88_08970"
     CDS             complement(455..1453)
                     /gene="hpnH"
                     /locus_tag="VFO88_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HET9545698.1"
                     /translation="MKFPLRSTVQIGRYVAQQKGRERYPLVIMLEPTLGCNIACIGCG
                     KIREYESNKARLTVEECLESARECPAPVISICGGEPLIFKGIEEVVAGFLEMRRNVQL
                     CTNALRLDDMLDRFEPDPRLTFVVHLDGMEEIHDWICDYPGLWEIAVGAIRTAREAGF
                     RVTTNTTIFKETSVDDVIEMMGYLTNEVGIDGMLVAPGYQYSQIDPSLTMTRSEHEEK
                     FRAIRAAVREHGYKWLASPVYQDFLTGQRKLDCAPWGSVTRNPYGWKGPCYLLTDGIF
                     PTYEALLHGMEWERYGPGNDHRCEHCAIHSGFEPAATLATTESARDTARTLAWTLG"
     gene            complement(1450..>2239)
                     /locus_tag="VFO88_08975"
     CDS             complement(1450..>2239)
                     /locus_tag="VFO88_08975"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9545699.1"
                     /translation="LDRPALERAFAGCDAVVHVAALYSFDARAEELERVNVAGTENVL
                     AACAAAGVRRLVHTSTAGTCGPVPGRAATEEDGPPEQELAVPYKRTKLAAEERVLAAA
                     RDGLDAVVVNPTAPVGEGDSKPTPTGRMIAGVASGRIPAYVASGLNVVDVRDVARGHV
                     LALERGRAGERYLLGGVNLTLAELFAAIARIAGRARPRVRVPYPVTVGAAQLGLANRD
                     EVRLARLPMFFSSAKAERELGYRPGPVEPALARAVAELAGKEAA"
BASE COUNT          292 a          835 c          815 g          297 t
ORIGIN      
        1 gcgagagcgt ccgtctcgcc ggcggacgtc accgcccccg cgagcggccc gagcgggacc
       61 gccggagtgt cggacacgac ccgcacgact ccggccaggc ggcccgtggc cgccaggcgg
      121 ccgctctcga gatcgacgac ctccgcgccg gaacgctccg cgagcgccgt ccgctcgccc
      181 gccgcgtcca cgatccgccc ggccgcgcag aggacgacgg cccgcgcgcc ggccacggcg
      241 cgcggcggcc cctcccacag cgcctccccc gcctcgtcga ccacgctccg tgccgtcacg
      301 atcgtcccgg ggggaagccc gtcgacgagg gcgccgcaga gcccgaacga cacgagcggc
      361 ccgtccggca gctccggtct gatccgtgcc acccgagctc cgccccgccg cgccgcccgc
      421 tcctcgacgg cgagcgcaca gacgagcgtg ggcgtcagcc gagcgtccag gcgagcgtgc
      481 gcgccgtgtc gcgcgcgctc tcggtcgtcg cgagcgtcgc ggccggctcg aagcccgagt
      541 ggatcgcgca gtgctcgcag cgatggtcgt tcccggggcc gtagcgctcc cactccatcc
      601 cgtggagcag cgcctcgtac gtcgggaaga tcccgtccgt caggaggtag cacgggccct
      661 tccagccgta gggattccgg gtcacggagc cccacggcgc gcagtcgagc ttgcgctggc
      721 cggtgaggaa gtcctggtag accgggctcg cgagccactt gtagccgtgc tcgcgcaccg
      781 cggcgcggat cgcgcggaac ttctcctcgt gctccgagcg cgtcatcgtg agggacgggt
      841 cgatctgcga gtactggtag cccggcgcga cgagcatccc gtcgatcccg acctcgttcg
      901 tcaggtagcc catcatctcg atcacgtcgt cgacggacgt ctccttgaag atcgtcgtgt
      961 tcgtcgtgac gcggaagccg gcctcgcgcg cggtgcggat cgcgccgacg gcgatctccc
     1021 agagcccggg gtagtcgcag atccagtcgt ggatctcctc catgccgtcc aggtggacta
     1081 cgaacgtgag ccgcgggtcc ggctcgaagc ggtcgagcat gtcgtcgagc cggagcgcgt
     1141 tcgtgcagag ctggacgttc cgccgcatct cgaggaaccc cgcgacgacc tcctcgatgc
     1201 ccttgaagat aagcggctcg ccgccgcaga tcgagatgac cggcgcgggg cactcgcgcg
     1261 cgctctcgag gcactcctcc acggtcaggc gtgccttgtt cgactcgtac tcgcggatct
     1321 ttccgcagcc gatgcaggcg atgttgcagc cgagcgtggg ctccagcatg atcacgagcg
     1381 ggtagcgctc gcggcccttc tgctgcgcca cgtagcggcc gatctggacg gtgctgcgga
     1441 gcgggaactt catgcggcct ccttcccggc cagctcggcc acggcacggg cgagcgccgg
     1501 ctcgacgggc ccggggcgat agccgagctc gcgctccgcc ttcgcggagg agaagaacat
     1561 cggcaggcgg gccaggcgaa cctcgtcccg attggcgagt cccagctgcg cggcgcccac
     1621 ggtgacaggg tacggcaccc tcacgcgcgg gcgcgcgcga ccggcgatgc gggcgatcgc
     1681 ggcgaagagc tcggcgaggg tcaggttcac gccgccgagc aggtagcgct cgcccgcccg
     1741 gcctcgctcg agcgcgagga cgtggccgcg ggcgacgtcg cggacgtcga cgacgttgag
     1801 gccggaggcg acgtacgccg ggatccgccc ggaggcgacg cccgcgatca tccgcccggt
     1861 cggggtcggc ttgctgtcgc cctcgccgac cggcgccgtc gggttgacga cgaccgcgtc
     1921 gagcccgtcg cgcgcggcgg cgaggacgcg ctcctcggcg gcgagcttcg tccgcttgta
     1981 cggcacggcg agctcctgct cgggcggacc gtcctcctcg gtcgcggccc ggccgggaac
     2041 cggcccgcac gtcccggcgg tgctcgtgtg cacgaggcgc cggacgccgg cggcggcgca
     2101 ggcggcgagg acgttctccg tcccggcgac gttcacgcgc tcgagctcct ccgcgcgcgc
     2161 gtcgaagctg tagagcgcgg cgacgtggac gacggcgtcg cagccggcga acgcccgctc
     2221 gagcgcggga cggtcgagg
//
LOCUS       DASTFC010000303         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_90330,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000303 DASTFC010000000
VERSION     DASTFC010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90330"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFO88_08980"
     CDS             <1..193
                     /locus_tag="VFO88_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545700.1"
                     /translation="AVEFDLPYGEWIRVFREAGLSVEVLVEVRPPEGAASTYRNAAET
                     EFARGWPMEQIWKARKTPA"
     gene            331..951
                     /locus_tag="VFO88_08985"
     CDS             331..951
                     /locus_tag="VFO88_08985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9545701.1"
                     /translation="MLGDVAGKDVVELGCGTAYFSAWLARRGARPVGVDVTPEQLATA
                     RALQAEHGLAFPLVEASAENVPLPSESFDLALSEYGASIWADPYRWVPEAARLLRPGG
                     ELVFLVHGVLLALCWPNFDDPAGEELARAYFGLRSLEDPADGATTFHLGYGEWIRLLR
                     ANGLEVERLLEVQAPESAETRWDFVTPEWARRWPSEEIWKARKRAA"
     gene            948..1544
                     /locus_tag="VFO88_08990"
     CDS             948..1544
                     /locus_tag="VFO88_08990"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET9545702.1"
                     /translation="MSAPPAPPLLLASTSPQRRAILEQLGLPFEVVAPAFEEDDEADV
                     PAVELVRGHARGKARSVAGRAAPGQPVLAVDTTVVLDGRVFGKPADAADAGRMLELLG
                     GRTHAVVSGLCLLTPAWEEVVDATTEVTFRPLAPRDLAWYVALGEWEGRAGGYAIQGR
                     GAALVERIEGDYLNVVGLPAAALVRLLAERFAGTYGFG"
     gene            complement(1541..1909)
                     /locus_tag="VFO88_08995"
     CDS             complement(1541..1909)
                     /locus_tag="VFO88_08995"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9545703.1"
                     /translation="MFPGPPHRVVHLELRTGNAARASAFHAELFGWSIEAVRTEGEPY
                     LAVDLGGRVGGGIVAHETPRPFWLPYVEVADVDVSVARAEALGAQVLLPPREGPAGWR
                     SVVAAPFAGVVALWQPKREV"
     gene            1993..>2779
                     /locus_tag="VFO88_09000"
     CDS             1993..>2779
                     /locus_tag="VFO88_09000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HET9545704.1"
                     /translation="MRAITAGAELTERELLAAACDGDSDAFRRLVEPHRTALHAHCYR
                     MLGSLHDSEDAMQEALLRAWRGLCRFDRRSALSSWLYRIATNVCLDALSRRSKRVLPI
                     DYGPALEDGGETGAAPVAEPVWIEPYPDEALGLGGGYAGPAARYEQREAVELAFVAAL
                     QHLPPRQRAVLILREVLGLTAREVGEMLGASVASVNSLLQRARRSIDERLPEQSQQET
                     LRSLGDERVRELVGQFVDAFERADVDAIVGLLADDATFAMPPYS"
BASE COUNT          335 a          990 c         1081 g          373 t
ORIGIN      
        1 ggcggtcgag ttcgacctcc cgtacgggga gtggatccgc gtcttccgcg aggcggggct
       61 ctcggtcgag gtgctcgtcg aggtgcgccc gcccgagggc gcggcgtcga cgtacaggaa
      121 cgcggccgag acggagttcg cgcgcggctg gccgatggag cagatctgga aggcccggaa
      181 gactccggcg tagggctctc ggcgcacgcc gagcgcaacc ggagcgtctg ggccgggctc
      241 gcccccgagt tcgccgagcg cgggcggcgc aactgggcga agcccgatgt cgactggggg
      301 atctgggacg ttcccgagac ggagctcagg gtgctcggag acgtggccgg gaaggacgtc
      361 gtcgagctcg gctgcgggac ggcgtacttt tccgcgtggc ttgcacgacg gggcgcgcgg
      421 cccgtcggcg tggacgtgac gccggagcag ctcgcgacgg cgcgggcctt gcaggccgag
      481 catgggctcg cgttcccgct cgtcgaggcg agcgccgaga acgtgccgct gccgtccgag
      541 tcgttcgacc tggccctgtc cgagtacggg gcgtccatct gggcagaccc ctaccggtgg
      601 gtgcccgagg cggcgcgcct cctgcgcccg ggcggcgagc tcgtcttcct cgtgcacggg
      661 gtcctgctcg cgctctgctg gccgaacttc gacgacccgg ccggggagga gctcgcgcgg
      721 gcgtacttcg gcctccggtc gctcgaggac ccggcggacg gcgcgacgac gtttcacctc
      781 ggctacggcg agtggatccg gctgctgcgc gcgaacgggc tcgaggtcga gcgcctcctc
      841 gaggtccagg ccccggagag cgcagagacg cgctgggact tcgtgacgcc ggagtgggcg
      901 cgccgctggc cctccgagga gatctggaag gcgcggaagc gggcggcatg agcgcgcctc
      961 ccgccccgcc gctcctcctc gcctccacgt cgccgcagcg gcgcgcgatc ctcgagcagc
     1021 tcgggttgcc cttcgaggtc gtcgcgcccg cgttcgagga ggacgacgag gcggacgtgc
     1081 ccgcggtcga gctcgtgcgc ggccacgcgc gcgggaaggc gcgctcggtc gcgggccggg
     1141 ctgcgccggg ccagccggtg ctcgccgtgg acacgaccgt cgtgctcgac gggcgggtgt
     1201 tcgggaagcc cgcggacgcc gcggacgccg ggcggatgct cgagctgctc ggcgggcgga
     1261 cccacgcggt tgtctccggc ctctgcctgc tcacgcccgc ctgggaggag gtcgtggacg
     1321 cgacgacgga ggtgaccttc cgcccgctcg cgccgcgcga tctggcgtgg tacgtggcgc
     1381 tcggcgagtg ggagggccgg gccggcggct acgcgatcca gggccggggc gccgccctcg
     1441 tcgagcggat cgagggcgac tacctgaacg tcgtcgggct cccggcggcc gcgctcgtcc
     1501 gcctcctggc cgagcgcttc gccggcacct acgggttcgg ctagacctct cgcttcggct
     1561 gccagagcgc gacgacgccg gcgaacggcg ccgcgacgac gcttctccag ccggccgggc
     1621 cctcgcgcgg cggcaggaga acctgcgctc cgagcgcctc cgcccgcgcg accgacacgt
     1681 cgacgtcggc gacctcgacg taggggagcc agaacggccg tggcgtctcg tgcgcgacga
     1741 tcccgccgcc cacccgcccg ccgaggtcga cggcaaggta cggctctccc tccgtccgga
     1801 cggcctcgat gctccagccg aagagctccg cgtggaaggc ggatgcgcgc gccgcgttcc
     1861 ccgtgcggag ctcgagatgg acgacccggt ggggcggacc tggaaacatt gctcgtcggg
     1921 ttggaccggc cggcgcggcc gaacgcatcg ccggggcgat gagtctgcgc tccgccgtcg
     1981 gtctgaatgg aggtgagggc gatcacggcc ggtgccgagt tgacggagcg ggagctgctc
     2041 gccgccgcgt gcgacgggga cagcgacgcc ttccgccgtc tcgtcgagcc ccaccgcacg
     2101 gcgctccacg cccactgcta ccgcatgctc gggtcgctcc acgattccga ggacgcgatg
     2161 caggaggcgc tcctccgcgc ctggcgcggg ctctgccgct tcgaccgccg gagcgcgctc
     2221 tcgagctggc tctaccggat cgcgacgaac gtctgcctcg acgcgctgtc gcgacgctcg
     2281 aagcgcgtgc ttccgatcga ctacggcccg gccctcgagg acggcggcga gacgggcgcg
     2341 gcgcccgtgg ccgagcccgt gtggatcgag ccgtatccgg acgaggcgct cggactgggg
     2401 ggcgggtacg ccgggccggc ggcgcgctac gagcagcggg aggcggtcga gctcgcgttc
     2461 gtcgcggcgc tccagcatct gccgccgcgc cagcgggccg tcctcatcct ccgcgaggtg
     2521 ctggggctca ccgcccgcga ggtcggcgag atgctcggcg cgtcggtcgc ctcggtgaac
     2581 agcctcctgc agcgggcgcg caggtcgatc gacgagaggc tccccgagca gagccagcag
     2641 gagacgctgc gctcgctcgg agacgagcgc gtgcgcgagc tggtcgggca gttcgtcgac
     2701 gcattcgagc gcgcggacgt ggatgcgatc gtcgggctcc tcgccgacga cgcgaccttc
     2761 gccatgccgc cgtacagcg
//
LOCUS       DASTFC010000304         5766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_485996,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000304 DASTFC010000000
VERSION     DASTFC010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485996"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VFO88_09005"
     CDS             complement(<1..783)
                     /locus_tag="VFO88_09005"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9545705.1"
                     /translation="MLDRFRMVEPLRSRDFAFYWAATTLALVADGIYYVAVAWQVYDL
                     SNAPTALSIVGVAWSVPMVVFVLWGGVAADRFDRRLLIALAGIARATAMSALAVLALT
                     GAIELWHIVGIVVLFGAGEAFAGPATGAIVPDLVPERLLVQANALGQLVEPLGWRIVG
                     PAVGGFAVASAGPGAAFTAAAACFVGSAVAVSLVRATARPRAEDAPGTSALREVAEGY
                     RFVRSQTWLWGTLLAAAVSLLAFAGPYEVLIPYLVRNELDGGA"
     gene            complement(801..1841)
                     /gene="rsgA"
                     /locus_tag="VFO88_09010"
     CDS             complement(801..1841)
                     /gene="rsgA"
                     /locus_tag="VFO88_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET9545706.1"
                     /translation="MSIERYGWSRELDEAFAPLRAEGLSPARVVVQQRGIYTVEADDG
                     ERSAEPSGRLRHEAAEGGLPVAGDWVGLRGTRIEAVLERRTAFVRKAAGEAAVQQVVA
                     ANFDLGLVVQALPDDVNPRRLERYVTAVWESGATPVVVLTKTDLADDVTAASLEVEAA
                     APGVSVHAISSVTGEGVEAIRALLAPNRTAVLVGSSGVGKSTLVNRLLGEERQAVAEV
                     RDDGRGRHTTTHRELLAVPGGGLLLDTPGMRELGLWADEESLDAAFDDVVELAAGCRF
                     SDCTHVHEPGCAVLAAVEDGRLEPARLESFHKLGRELAYLERRTDARLQAEEKKKWRA
                     VQMEYRRQTRDR"
     gene            2122..2652
                     /locus_tag="VFO88_09015"
     CDS             2122..2652
                     /locus_tag="VFO88_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545707.1"
                     /translation="MGPDGRERAWVRGAQAGRASDLEALFSRHWRPAYRAAYLVVHDA
                     AAAEDIAQEAFLSAVRHLDRFDRRRPFGPWLHRIVVNRAIDWARARAARDEASPEPLE
                     QLGADDSPGEGLSPHVVEALHLLSPEHRAVIVLRYLLDYTPGEIGELLELPRGTVNSR
                     LRRGLDALAERLGEAR"
     gene            2649..3824
                     /locus_tag="VFO88_09020"
     CDS             2649..3824
                     /locus_tag="VFO88_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545708.1"
                     /translation="MTERRLRELLRADPPPGELDAERRAWHVVAAAFAEREPVPPPRR
                     HVRALALGVAALALVAAALSPPGSAVLDSIRNAVGRERVVGVEEARPLLLELPSPGRL
                     LVTSPSGAWVVNADGSKRLLGEARDATWSPQGLFVGAARGRQLVALTPEGVTRWTIPQ
                     RARVREPRWAPSGFRIAYLAGSTLRVTAGDGTGDRLLAEGVPGIAPAWRPGARHVVAY
                     ATSGGGVWVVDTDADRVFLRVAATGGPVLGLAWLAGGRTLAVLDRDGVRLVGSDGATR
                     RIPLAGRAGVTLAAAPAGGRLAVVARSRGGRSDLLLVGTGRGEVRSIFSGAGRFTEAA
                     WSPDGEWLLVAWASADQWVFVRAADDGGLAAVGNVSEQFVGNAEAEEGFPRLGGWCC"
     gene            complement(3829..4473)
                     /locus_tag="VFO88_09025"
     CDS             complement(3829..4473)
                     /locus_tag="VFO88_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545709.1"
                     /translation="MTDDLRLTVAFASEDDARELVRWLHEARLDAADRERLGERVIVS
                     RDDASVFVYVGSEEEAHEIRRLIEARVGHPAAARVALDRWHPVEQGWQDASIPLPRTE
                     EELAAEHERRQAREAIESHRSGYAAWEVRVELASPEATAELGDRLEAEGIPVVRRQTF
                     LLVGAVNEDQAHALADRLAEETPDDARIEVEPGGRMVWEVVPSNPFAVFGGLGV"
     gene            4516..5469
                     /locus_tag="VFO88_09030"
     CDS             4516..5469
                     /locus_tag="VFO88_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9545710.1"
                     /translation="MHWLVKRTLQNVLGVLPSPQSANYFFQRHVTRTLPIGDAGVARK
                     FARALSHVAAYEEHGAGRPLDETVAYEFGAGWDLAIPLSYAALGVGRQVLVDIRPNLR
                     LELVNATLASLARQREALEEVAGRELRDLGPPGAESAEDLRARFGIEYRAPCDAAATG
                     LPAESVDLVTSTNTLEHVPREAIAPILREAARLLRPDGVMSFRIDMQDHASYADPRRS
                     PYDYLRYSERAWRFLSPKLEYENRLRLPDYRALFAEAGLEIAEETVTRPRPEHLAALA
                     EVPLARRFRRYAPEDLAARAVAVVLRARSPDRAEQATDLVG"
     gene            complement(5428..>5766)
                     /locus_tag="VFO88_09035"
     CDS             complement(5428..>5766)
                     /locus_tag="VFO88_09035"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9545711.1"
                     /translation="GLAHAVAQALGGRFGIPHGAANALSLAPALRFNEPVAGAEIARL
                     AEAMGVDDAADRAEELARLAGFERLRDLGVPEEGLEEVAELAAARPGNRANPRQASAD
                     EIRGLLRAIW"
BASE COUNT          668 a         2206 c         2180 g          712 t
ORIGIN      
        1 ggcgccgccg tcgagctcgt tccggacgag gtacgggatg agcacctcgt acgggccggc
       61 gaacgcgagc agcgacacgg cggccgcgag cagcgtcccc cagagccacg tctgggagcg
      121 gacgaagcga tagccctcgg caacctcgcg gagcgcgctc gtgcccggcg cgtcctcggc
      181 gcgcgggcgg gcggtcgcgc gcacgagcga gacggcgacg gcggagccga cgaagcatgc
      241 ggcggccgcg gtgaacgcag cgcccggacc ggccgacgcg accgcgaagc cgccgacggc
      301 cggccccacg atcctccagc cgagcggctc gacgagctgc ccgagcgcgt tcgcctgcac
      361 gagcagccgc tccgggacga ggtcggggac gatcgcgccg gtcgcgggcc cggcgaacgc
      421 ctcgccggcg ccgaagagga cgacgatccc gacgatgtgc cagagctcga tcgcgccggt
      481 gagcgcgagg acggcgagcg cgctcatcgc ggtcgcccgc gcgatccccg cgagcgcgat
      541 caggagccgc cggtcgaacc ggtccgcggc gacgccgccc cacaggacga agacgaccat
      601 cggcacgctc cacgcgacgc cgacgatcga gagcgccgtc ggggcgttcg acaggtcgta
      661 gacctgccac gcgaccgcga cgtagtagat cccgtcggcg acgagcgcga gggtcgtcgc
      721 cgcccagtag aacgcgaagt cgcggctgcg gagcggctcg accatgcgga agcggtcgag
      781 catccgccgg ctctcggtct ctaccggtcc cgtgtctgcc tccggtactc catctgcacc
      841 gcgcgccact tcttcttctc ctcggcctgc aggcgggcgt cggtgcgccg ctcgaggtac
      901 gcgagctcgc gcccgagctt gtggaagctc tcgagccgcg ccggctcgag gcggccgtcc
      961 tcgaccgcgg cgagcaccgc gcagcccggc tcgtgaacgt gcgtgcagtc cgagaagcgg
     1021 cagccggccg cgagctcgac gacgtcgtcg aaggccgcgt cgagcgactc ctcgtccgcc
     1081 cagagcccga gctcgcgcat ccccggcgtg tcgaggagca gcccgccgcc cggcaccgcg
     1141 agcagctcgc ggtgcgtcgt cgtgtgccgg ccgcgcccgt cgtcgcgcac ctccgcgacc
     1201 gcctggcgct cctcgccgag cagccggttc acgagcgtcg acttcccgac gccggacgag
     1261 ccgacgagga cggcggtgcg gttcggggcg agaagcgcgc ggatcgcctc cacgccctcc
     1321 ccggtcaccg agctgatcgc gtggacggag acgcccgggg cggccgcctc gacctcgagc
     1381 gacgcggccg tgacgtcgtc cgcgaggtcg gtcttcgtca ggacgacgac gggcgtcgcc
     1441 ccgctctccc agaccgcggt cacgtagcgc tcgagccggc gcgggttcac gtcgtccggc
     1501 agcgcctgga cgacgagccc gaggtcgaag ttcgcggcca cgacctgctg gacggccgcc
     1561 tcgcccgcgg ccttgcggac gaaggcggtg cggcgctcga ggaccgcctc gatccgcgtc
     1621 ccgcgcaggc cgacccagtc gcccgcgacg gggagcccgc cctcggccgc ctcgtggcgc
     1681 aggcgccccg acggctcggc cgaccgctcc ccgtcgtcgg cctccacggt gtagatcccg
     1741 cgctgctgga cgacgacgcg ggcaggcgag agcccctccg cgcgcagcgg cgcgaaggcc
     1801 tcgtccagct ccctgctcca gccgtatctc tcgatgctca aggtgtgtcc tcctgcctct
     1861 gcgaagggtt gacggcgcgc ccgtgggcgc gcatgggtcg cgtcgggtcc cggcgctgcg
     1921 gcttgccgct agccgaagac cggcaggccg cgacgggcgg cgatgcggac aagtgctctc
     1981 acgatgtcgc ctcctcggtc gcgggtcagg acgccgtgag ggtagcggac gcgccggggc
     2041 gtgtcacgga cctcgtcggc cgcgccgccc gtagagacgg caggccgttg tgggaggaac
     2101 gcttcgtata gtcaggtgcc gatggggccg gacgggcggg agcgcgcctg ggttcgcggc
     2161 gcccaggcgg gacgggcctc cgatctcgag gccctgttct cgcgccactg gcggccggcg
     2221 taccgggccg cctacctcgt cgtgcacgac gccgcggccg cggaggacat cgcgcaggag
     2281 gcgttcctct ccgccgtgcg gcacctcgac cgcttcgacc gccgtcgtcc gttcgggccg
     2341 tggctccacc ggatcgtcgt caaccgcgcg atcgactggg cgcgcgccag ggcggcgcgc
     2401 gacgaggcct cgcccgagcc gctcgagcag ctcggcgccg acgattctcc cggcgagggg
     2461 ctctcgccgc acgtcgtgga ggcgctccac cttctctcgc cggagcaccg cgcggtgatc
     2521 gtcctgcgct acctcctcga ctacacgccg ggcgagatcg gcgagctgct cgagctcccg
     2581 cgcgggacgg tcaactcgcg gctgcggcgc gggctcgacg ccctcgccga gcggctgggg
     2641 gaggcgcgat gaccgagcgg cggctgcgcg agctcctgcg ggccgacccg ccgccgggcg
     2701 agctcgacgc cgagcggcgc gcatggcacg tcgtcgcggc cgcgttcgcc gagcgcgagc
     2761 cggtgccgcc gccgcgccgg cacgtccgcg cgctcgcgct cggggtggcc gcgctcgcgc
     2821 tcgtggccgc ggcgctcagc ccgccgggct cggccgtcct cgactcgatc cggaacgcgg
     2881 tcggccgcga gcgcgtcgtc ggggtcgagg aggcccgtcc gctgctcctc gagctcccct
     2941 cgcccggccg gctgctcgtg acgtcgccga gcggggcctg ggtcgtgaac gcggacggct
     3001 cgaagcgcct gctcggggag gcgcgggacg cgacctggtc gccgcagggc ctcttcgtcg
     3061 gggccgcgcg cgggcgccag ctcgtcgcgc tgacgccgga gggcgtgacg cgctggacga
     3121 tcccgcagcg cgcccgcgtc cgcgagccgc ggtgggcgcc cagcgggttc aggatcgcct
     3181 acctcgccgg gtcgacgctc cgcgtcaccg ccggggacgg aacgggcgac cgcctgctcg
     3241 cggagggcgt ccccggcatc gcgccggcct ggcggccggg ggcgcgccac gtcgtcgcgt
     3301 acgcgacgag cggcggcggc gtttgggtcg tggacacgga cgccgaccgc gtgttcctcc
     3361 gcgtcgcggc gaccggcggg cccgtgctcg gcctcgcctg gctcgcgggc ggccggacgc
     3421 tcgccgtgct cgaccgcgac ggggtgcgac tcgtggggtc ggacggcgcg acgcggagga
     3481 tcccgctcgc cgggcgggcc ggcgtcacgc tcgcggccgc accggccggc ggccggctcg
     3541 cggtcgtcgc ccggagccga ggcggccgga gcgacctcct gctggtcggc accggcaggg
     3601 gagaggtgcg ctcgatcttc tccggcgccg gccgcttcac cgaggccgcg tggtcgccgg
     3661 acggcgagtg gctgctcgtc gcgtgggcga gcgccgacca gtgggtcttc gtccgcgccg
     3721 ccgacgacgg cgggctcgcg gcggtcggca acgtctcgga gcagttcgtg gggaacgccg
     3781 aggccgagga aggcttcccg cggctcggcg gctggtgctg ctgacccgct agaccccgag
     3841 gccgccgaag accgcgaacg ggttcgaggg gacgacctcc cagaccatcc gcccgccggg
     3901 ctcgacctcg atgcgggcgt cgtcgggcgt ctcctccgcg aggcggtctg cgagcgcgtg
     3961 cgcctggtct tcgttcaccg cgccgacgag gaggaacgtc tggcggcgca cgacggggat
     4021 tccctccgcc tcgagccggt cgccgagctc ggccgtcgcc tcgggcgagg cgagctcgac
     4081 ccggacctcc cacgcggcgt agccggagcg gtgcgactcg atcgcctcgc gcgcctggcg
     4141 gcgctcgtgc tcggcggcga gctcctcctc cgtccggggc agcgggatcg acgcgtcctg
     4201 ccagccctgc tccaccggat gccagcggtc gagcgcgacc cgcgcggcgg ccgggtgacc
     4261 cacccgggcc tcgatcagcc gccggatctc gtgcgcctcc tcctcgcttc cgacgtagac
     4321 gaagaccgac gcgtcgtcgc ggctcacgat cacccgctcc ccgagccgct cgcggtcggc
     4381 ggcgtcgagc cgcgcctcgt gcagccagcg cacgagctcg cgcgcgtcgt cctcgctcgc
     4441 gaacgcgacg gtgagtcgga ggtcgtcggt cacggcggca ttctgcccgt tcggcccggg
     4501 taggctcatt cgaagatgca ctggctcgtg aagcggacgc tccagaacgt gctcggcgtc
     4561 ctcccgtcgc cccagtcggc gaactacttc ttccagcgcc acgtgacccg gacgctgccg
     4621 atcggcgacg ccggcgtggc gcgcaagttc gcgcgcgcgc tctcgcacgt ggccgcctac
     4681 gaggagcacg gcgccgggcg gccgctcgac gagacggtcg cctacgagtt cggcgccggc
     4741 tgggacctcg cgatcccgct gagctacgcg gcgctcgggg tcgggcgcca ggtgctcgtc
     4801 gacatccggc ccaacctgcg gctcgagctc gtgaacgcga cgctcgcgtc gctcgcccgg
     4861 cagcgggagg cgctcgagga ggtcgccggc cgcgagctgc gcgacctcgg ccctcccggc
     4921 gccgagtcgg ccgaggacct gcgggcgcgc ttcgggatcg agtaccgcgc gccgtgcgac
     4981 gcggcggcga ccgggctccc ggccgagtcg gtcgacctcg tgacgagcac gaacacgctc
     5041 gagcacgtcc cgcgcgaggc gatcgcgccg atcctgcgcg aggcggcgcg gctcctgcgg
     5101 cccgacgggg tcatgagctt ccggatcgac atgcaggatc acgcctcgta cgccgacccg
     5161 cgccgctcgc cgtacgacta cctccgctac tcggagcgcg cgtggcggtt cctctcgccg
     5221 aagctcgagt acgagaaccg cctgcggctg ccggactacc gggccctctt cgccgaggca
     5281 ggcctcgaga tcgcggagga gacggtcacg cggccgcggc ccgagcatct cgccgcgctc
     5341 gccgaggtcc cgctcgcgcg gcgcttccgc cgatacgcgc ccgaggatct cgccgcgcgc
     5401 gcggtcgccg tcgtgctcag ggcacgctca ccagatcgcg cggagcaggc cacggatctc
     5461 gtcggctgac gcctgccgcg ggttcgcgcg gttgcccggg cgggcggcgg cgagctcggc
     5521 gacctcttcg aggccctcct cggggacgcc gaggtcgcgc agccgctcga agccggcgag
     5581 ccgggcgagc tcctcggcgc ggtcggccgc gtcgtccacg cccatcgcct cggcgagccg
     5641 ggcgatctcc gcgcccgcga cgggctcgtt gaagcggagc gccggcgcga gcgagagcgc
     5701 gttcgcggcg ccgtgcggga tcccgaagcg gccgccgagc gcctgcgcga cggcgtgcgc
     5761 gagccc
//
LOCUS       DASTFC010000305         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1177929,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000305 DASTFC010000000
VERSION     DASTFC010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177929"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFO88_09040"
     CDS             <1..885
                     /locus_tag="VFO88_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545712.1"
                     /translation="GLGSQDAVIVSDDGRLLFAVNAGSGSVSAFRIRAHGLELAGIAP
                     SGGPMPTSLTYRKGLLYVLNAGVPNSISGLSVGGDGSLAPLPGSTRPLSAASTAPAEV
                     NWSDGGDALVVTERATNRLTSYAVGADGLPSGAAVVTPSSGPTPFGFAITKRDTVFVS
                     EAGAGGGASTYALGAGAVLTPVSSMLMTGQRAACWAVATRNGRFGYVTNAGTGNISGF
                     ALAQDGSGSLLDAHGVTAVTGGNPTDAALSHDSTYLYARVAALGRIAIFRVAADGSLA
                     ERPALEGAPAGLAGLAAW"
     gene            complement(1310..1675)
                     /locus_tag="VFO88_09045"
     CDS             complement(1310..1675)
                     /locus_tag="VFO88_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545713.1"
                     /translation="MPSLDRLPPVLTACPAAAALALAAVAEQAGAGTAVFYLLLAGVP
                     LAAVAALAAVARLVDRGHGRLEVGLAAALVVALCVGAAARSPVFPGGEAGPVLALALA
                     CGWAAVAVLAALTFGSLRR"
     gene            1749..2450
                     /locus_tag="VFO88_09050"
     CDS             1749..2450
                     /locus_tag="VFO88_09050"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9545714.1"
                     /translation="MAGVKLTWLGHAAFRVEAPSGRRLYVDPWLGNPKCPENEREPER
                     IDVIALTHGHDDHVGETVELAQRFGCPVYAQVELRGWLAGKGLPEEPTQAMNKGGTVD
                     ADGITITMTDANHSSSQFENGAPVYLGEPAGLVIGVEGAVRIYFAGDTNVFGDMELIG
                     RLYEPDLVVLPIGDHYTMGPKEAALAVELLGATRMVPCHYGTFPLLTGTPDACRERMR
                     GCEVVAPAPGETIEL"
     gene            2450..2731
                     /locus_tag="VFO88_09055"
     CDS             2450..2731
                     /locus_tag="VFO88_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545715.1"
                     /translation="MRERWFGATGRLVPEIAVEGDPAVPVEEALVVDGVEDVAALEEA
                     HRAGRPVAVRCADEAAVRRALARPEVSCALVPPSRRDLLDLDLRRLTYG"
     gene            2736..3572
                     /locus_tag="VFO88_09060"
     CDS             2736..3572
                     /locus_tag="VFO88_09060"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET9545716.1"
                     /translation="MRPVSTYSLCACDLERGEWGVAVQSKFLAVGSIVSWAEAEVGCV
                     ATQAWANPLYGPEGLALLREGRTADEVVQALVGADEGRDDRQLGVVDGRGGSATFTGS
                     ACFDWAGGRTGPGWAAQGNLLVSAATVDALAETFAASVGPLAERLLAALAAGEAAGGD
                     RRGRQSAALLVVRREGGYGGMTDVAVDLRVDDHPEPIEELARLYGLHELYFGSTPEEE
                     WLPVDDGLREELRQRLAALGYDATDLDEAFRAWTGAENLEDRVSGLDRLDPVVLAELR
                     KR"
BASE COUNT          501 a         1364 c         1481 g          493 t
ORIGIN      
        1 gggctcggct cgcaggacgc cgtgatcgtg agcgacgacg ggcgcctgct cttcgccgtc
       61 aacgcgggct cggggtccgt ctcggccttc cgcatccgcg cgcacggcct cgagctcgcg
      121 ggcatcgcgc cgtccggcgg cccgatgccg acgagcctga cgtaccgcaa ggggctcctg
      181 tacgtcctga acgcgggcgt cccgaactcg atctccgggc tctcggtcgg aggcgacggc
      241 tcgctcgcgc cgcttcccgg ctcgacgcgc ccgctcagcg ccgcctcgac cgctccggcc
      301 gaggtcaact ggagcgacgg cggcgacgcg ctcgtcgtga ccgagcgggc gacgaaccgc
      361 ctcacctcgt acgcggtggg cgcggacggc ctcccgtccg gcgcggcggt cgtcaccccg
      421 tcgagcggcc cgacgccgtt cggcttcgcg atcacgaagc gcgacacggt cttcgtctcc
      481 gaggcgggtg ccggaggcgg cgcgtcgacg tacgcgctcg gcgccggcgc cgtgctcacc
      541 ccggtgagct cgatgctcat gaccgggcag cgcgccgcat gctgggccgt cgcgacgagg
      601 aacggccggt tcggctacgt gacgaacgcg ggcaccggga acatctccgg cttcgccctc
      661 gcgcaggacg ggtcggggtc gctcctcgac gcgcacggcg tgacggccgt caccggcggg
      721 aacccgacgg acgccgcgct ctcgcacgat tcgacgtacc tctacgcgcg ggtcgctgcg
      781 ctcgggcgga tcgcgatctt ccgggtcgcc gccgacggct cgctcgccga gcggccggcg
      841 ctcgagggcg cgcccgccgg gctcgcgggc ctcgccgcct ggtgagaaaa gggacgcggg
      901 ggagaagctg ccgcatcgct ctcccccgcg tccaccgcgg cggccggttc tgtcgtcgac
      961 tcggcccctt cccgtagccg ttcgatacgg ctccgtgtcg gcgacccgac cgtccgcgag
     1021 ccccgccggt cgactgccca tgaaatcgac ctgtcgccgg gctgtgccgg cctcggaacc
     1081 ggagccgggc cgtcgggacg ggagagactc gacggagcgg cgaccggatc gtaaccggca
     1141 accttattcg cccttacctc ctggttggca agggctatgt catgggtgtt gtcgagcgcg
     1201 gttgccccgt gagcttgaac tgcgaaaagg taataattgc tgagcttccg cgacacattt
     1261 gagcgcgaac cgtagcgctg acagacggaa cgcgcaagtc tgccgaaagc taacgacgca
     1321 gcgagccgaa cgtcagggcg gcgagaacgg cgacggcggc ccatccgcac gcgagcgcga
     1381 gggcgagcac ggggccggcc tctccgcccg ggaagacggg cgagcgcgcc gcggctccga
     1441 cgcagagcgc gaccacgagc gcggccgcga gccccacctc gagccggccg tgcccgcggt
     1501 cgacgaggcg ggcgacggcg gcgagcgcgg cgaccgccgc gagcggcacg ccggcaagca
     1561 ggaggtagaa gaccgccgtg ccggcgcccg cctgctccgc gaccgctgcg agcgcgagcg
     1621 cggcggcggc cgggcaggcg gtcagcacgg gcggcaggcg gtcgagcgag ggcacgggcg
     1681 gtccgggttc gtcgcttcgc cggaggttcc tccgttaccc tcgtcgggaa cggcgacaag
     1741 gaggacggat ggcaggcgtc aagctcacct ggctcggcca cgcggcgttc cgcgtcgagg
     1801 cgccgagcgg ccggcggctc tacgtcgacc cgtggctcgg gaacccgaag tgcccggaga
     1861 acgagcgcga gccggagcgc atcgacgtca tcgccctcac ccacggccac gacgaccacg
     1921 tcggcgagac ggtcgagctg gcgcagcggt tcggctgccc ggtctacgcg caggtcgagc
     1981 tgcgcgggtg gctcgcgggg aagggcctgc cggaggagcc gacgcaggcg atgaacaagg
     2041 gcggcaccgt cgacgcggac gggatcacga tcacgatgac ggacgcgaac cactcctcga
     2101 gccagttcga gaacggtgcg cccgtctatc tcggcgagcc ggcggggctc gtgatcgggg
     2161 tcgagggcgc cgtgaggatc tacttcgcgg gcgacacgaa cgtcttcggg gacatggagc
     2221 tgatcggccg cctctacgag cccgacctcg tcgtgctccc gatcggcgac cactacacga
     2281 tgggcccgaa ggaggccgcg ctcgccgtcg agctgctcgg ggcgacgcgg atggtgccct
     2341 gccactacgg gacgttcccg ctcctcaccg ggacgccgga cgcgtgccgc gagcggatgc
     2401 gcgggtgcga ggtcgtcgcc ccggcgcccg gcgagacgat cgagctctga tgcgggagcg
     2461 ctggttcggc gcgacggggc gtctcgtgcc ggagatcgcg gtcgagggcg acccggcggt
     2521 tcccgtcgag gaggcgctcg tcgtcgacgg cgtcgaggac gtggcggcgc tcgaggaggc
     2581 gcaccgcgcc gggcggccgg tcgccgtccg ctgcgccgac gaggcagcgg tgcggcgcgc
     2641 gctcgcgcgt cccgaggtct cgtgcgcgct cgtcccgccg tcgcgccggg acctgctcga
     2701 cctcgatctg cgccggctca cgtacggcta acggcgtgcg cccggtctcg acctactcgc
     2761 tctgcgcctg cgacctcgag cgcggcgaat ggggcgtcgc cgtccagtcg aagttcctcg
     2821 cggtcgggtc gatcgtctcc tgggcggagg ccgaggtcgg ctgcgtcgcc acgcaggcgt
     2881 gggcgaaccc gctctacggg ccggaggggc tcgcgctgct ccgcgagggg cgcacggcgg
     2941 acgaggtcgt gcaggcgctt gtcggcgcgg acgaagggcg ggacgaccgc cagctcggcg
     3001 tcgtggacgg gcgcggcggc tcggcgacgt tcacgggctc ggcgtgcttc gactgggccg
     3061 ggggtcggac ggggcccgga tgggcggcgc aggggaacct cctcgtctcc gccgcgacgg
     3121 tggacgcgct cgccgagacg ttcgcggcgt cggtcgggcc gctcgccgag cggctgctgg
     3181 cggcgctcgc ggccggcgag gcggcgggcg gcgaccggcg cgggcggcag tcggcggcgc
     3241 ttctcgtcgt gcgacgcgag ggcggctacg gcggcatgac cgacgtggcg gtcgacctcc
     3301 gcgtggacga ccatccagag ccgatcgagg agctcgcccg gctctacggc ctccacgagc
     3361 tctacttcgg ctcgacgccg gaggaggagt ggctgccggt cgacgacggg ctccgggagg
     3421 agctccggca gcgcctcgcc gcgctcggct acgacgcgac cgacctggac gaggcgtttc
     3481 gcgcctggac gggcgccgag aacctcgagg accgtgtcag cggcctcgat cgcctcgacc
     3541 cggtcgtcct cgccgagctt cgcaagcgct gagcgaccgc cggagcccgt tcccttgtaa
     3601 cacggtgtta caagggaggg accctttccc ccggacggga gcatggggtt agtgacactc
     3661 tgttacaagg atcgggtcac cggtcgccgg gaagccgcct gcctgcggcc gcggcgcgga
     3721 gggccggtac cgggtcggtc tccgggcgcg cgacgacgag cagcgcgccg tggccgggcc
     3781 cggcgagcgc ctcgacgagc atctccaggc cgacggcgag cgcgaaggcc ttgtgcgcg
//
LOCUS       DASTFC010000306         7172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_123010,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000306 DASTFC010000000
VERSION     DASTFC010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123010"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFO88_09065"
     CDS             complement(<1..973)
                     /locus_tag="VFO88_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545717.1"
                     /translation="MAVATAEAVRARAAGLRRPLVRGAQLLAVSSFAVTQPLLDVLGK
                     NAEFFAVRGSPPRDIVLFALAVAFVLPAALLAVEIVVGAVSERAGAVCHLVFLAALGF
                     LFALHALGELGVERTEAVFGGALVAGAGLAAAVARVPFVRSFVTVLAPAPLVFLGFFL
                     FDSPASELVFPPDAEVRLAASDSTAPVVVLVLDELPTNSLLDERGEIDAGRYPSFARL
                     AESSTWFRNSSTRSASTSVAVPGLLTGNAPRSRTLPVFANYPDNLFTLLGGSHRLNVL
                     ETQTRVCPAELCEHRLEPARERFRSLFSDARVVYLYLVSPPALEDRLP"
     gene            complement(976..2316)
                     /locus_tag="VFO88_09070"
     CDS             complement(976..2316)
                     /locus_tag="VFO88_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545718.1"
                     /translation="MYALDGRVLRALGPDGLDGWRALAGSELFAAAVAEGKLVATEEV
                     DERLPEEAGAAAVLEHERIPFVSYPYEWSFSMLRDAALLQLELVRRALDEDLTLKDAS
                     PYNVQWRGTSPVFIDVGSFERLREGEPWQGYRQFCLLFLNPLLLQAYTGAPFQPWLRG
                     SLDGIHPDELRGVLSGRHRLRPGVLAHVVLHARLERRHGDGERDVKAELARAGFRKEL
                     VAANVRRLEKLVRRLRWEPGPTTWSDYGATTSYTEDDAARKESFVREVAARRRRGLVW
                     DVGANEGRHARIAAEHADTVVAFDADAAVVDRLYRELAAEGATRILPLVGDVTDPSPG
                     LGWRGRERLPLEERGRPELTLCLALLHHVSIAGNVPVTAFLDWLADLRTEIVLELPTR
                     DDPRVAALLARKRPGAHPDYDREPFERALAERFEVERSLELAGATRILYAARPR"
     gene            complement(2401..2577)
                     /locus_tag="VFO88_09075"
     CDS             complement(2401..2577)
                     /locus_tag="VFO88_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545719.1"
                     /translation="MRFAYLDPGTGSMIVQVALGGLAAVAVFFKMFWHRFRELVRIRK
                     PRPAERRDADTTTP"
     gene            complement(2691..4715)
                     /locus_tag="VFO88_09080"
     CDS             complement(2691..4715)
                     /locus_tag="VFO88_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9545720.1"
                     /translation="MTPVQPSVRRVGAQLLAATGLGVALPLLSALGDDPAFFAARGSP
                     ALDIWLFGLAVVLVPPLVLTALVVLVRAANAEAGAWLHVGLVGALAGLFALWGLERLG
                     VESTVALVVLGVLAAAGAGLAVAQARSVGTVLAFLAPLPLVALGLFLAVSPVSELALP
                     ADTGVEIAGVRSATPVVVVVFSELPTVSLLDPAGELDEGRFPAFASLARSSTWFRNAT
                     TVSATGDEAVPALLTGKRPEPGRLPIFQNHRENLFTLLGADYRLNVQETQTRLCPPEL
                     CERRDGREGTGVGGLLGDAGATWGRVVAPPGLESRLPEPDPERSSFADTGIFDAERER
                     SFRDFLRSLRYEPGEEPTLDVIHLRLPQGPWTRFPNGSQSAIPGEAPARNGEAEALQA
                     WQRHLLQAGYADRLLGQLVERMREVGLWENALLAVVVDHGIAFRPDAAGGTVTQDTLA
                     EIAFVPFFLKLPGQSTGRVEDRHVTSVDVLPTIAAALSVPVPWETDGASGLVRRRGAE
                     EVRVGGVTAPFGEALGARQAALAARVDLLGSGAWDGELYGPGPSGRLVGLTVGEFGVS
                     AAVEGEAVLSARLAGLLPSLPERSALVPSPIAGTVTGGVRPGDAIAVAVNDVVEAVVT
                     ATGEAGAARFSALVPPGAFLRDADNDVRVFLLGGSAGEPTLAEIETRLGG"
     gene            4909..6441
                     /gene="atpA"
                     /locus_tag="VFO88_09085"
     CDS             4909..6441
                     /gene="atpA"
                     /locus_tag="VFO88_09085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET9545721.1"
                     /translation="MKIRPEEITSILKQRIEEYDVETDLAEVGTVLQIGDGLARVYGL
                     ENCMAMEMLELEHGVVGVAFNLEEDNVGSPLFGDWELISEGAPVKRTGKVASVPVGEA
                     LVGRIVNPLGQPLDGQGPIKTTETRPLEFKAPGVVQRQPVTEPVQTGIKAIDSMIPIG
                     RGQRELIIGDRSTGKTALLVDTILNQKGQDMICVYVAIGQKASTVAQVYERLKDAGAM
                     DYTIIVSASATEAAPIKWMAPFAGCAMGEHFLYNGNHALCMYDDLSKHADAYRQMSLL
                     LRRPPGREAFPGDVFYLHSRLLERAVKLNDELGGGSLTALPVIETQAGDVSAYIPTNV
                     ISITDGQIFLESSLFYSGVRPAINVGISVSRVGGNAQTKAMKKVAGRLRLDLAQYREL
                     EAFAQFGSELDPQTQRTLARGERMVATLNQPQYAPWPLEEQVAAIFAGINGYLDDVPP
                     GQVPRFQEELREHLRTEGSVYKDIREGKELPDELADRLKAEIDKFKGNFAVREESAVP
                     GT"
     gene            6442..>7172
                     /gene="atpG"
                     /locus_tag="VFO88_09090"
     CDS             6442..>7172
                     /gene="atpG"
                     /locus_tag="VFO88_09090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET9545722.1"
                     /translation="MATVQDIKRRVSSVKNTSKITKAMELVAAAKLRKAEARIEALRP
                     YADRMRELMIGTARATPSRGFPLLEERENVSSVAILSLTGDRGLAGGFNAQIIRRSLA
                     LERQVRGDGADVRWLVVGKKGRSTLRFRRYDVAHAWVDLTDRPTYADAAAVANAVAEL
                     YTEREVDRVVMVYNRYLSALTQRVEVEEVLPIPRKALEEDEDRSAYEIALEGDFIYEP
                     EPDVILGRLLPTYLETSIYRALLESA"
BASE COUNT         1089 a         2576 c         2683 g          824 t
ORIGIN      
        1 cgggcagccg gtcctcgagc gcgggcgggg agaccaggta gaggtagacg acccgtgcgt
       61 ccgagaagag cgaccggaac cgctcccgcg ccggctcgag acggtgctcg cagagctccg
      121 cgggacacac gcgcgtctgc gtctccagca cgttcagccg gtggctgccg ccgaggagcg
      181 tgaagaggtt gtccgggtag ttcgcgaaga ccgggagggt ccggctccgc ggcgcgttgc
      241 cggtgaggag cccgggcacg gcgacggacg tcgaggccga ccgcgtcgag ctgttccgga
      301 accaggtcga cgactccgcg aggcgcgcga agctcgggta gcggcccgcg tcgatctcgc
      361 cccgctcgtc gaggagcgag ttcgtcggca gctcgtcgag gacgaggacg acgaccggcg
      421 ccgtcgagtc cgacgctgcg agccggacct ccgcgtcggg cgggaagacg agctcggacg
      481 ccggcgagtc gaagaggaag aagccgagga agacgagcgg cgcgggcgcg agcaccgtga
      541 cgaagctccg cacgaacggc acacgcgcga ccgcggcggc gagccccgcg cccgcgacga
      601 gcgcgccccc gaagacggcc tccgtccgct cgacgccgag ctcgccgagc gcgtggaggg
      661 cgaagaggaa gccgagcgcc gcgaggaaga cgaggtggca gacggcgccc gcccgctcgc
      721 tcaccgcgcc gacgacgatc tccaccgcga ggagcgccgc ggggaggacg aacgcgaccg
      781 cgagcgcgaa gaggacgatg tcgcgcggcg gcgagccgcg gacggcgaag aactccgcgt
      841 tcttccccag cacgtcgagc agcggctgcg tcacggcgaa gctcgacacg gccaggagct
      901 gcgcgccgcg gacgagcggg cgccggagcc cagcggcgcg ggcccggacg gcctccgcgg
      961 tcgcgaccgc cacggctacc gcggccgtgc cgcgtagagg atccgggtgg cgccggcgag
     1021 ctcgagcgag cgctcgacct cgaagcgctc cgcgagggcg cgctcgaacg gctcacggtc
     1081 gtagtcgggg tgcgcgccgg gccgcttccg ggcgagcagc gcggcgacgc gcgggtcgtc
     1141 gcgcgtgggg agctcgagga cgatctccgt ccggaggtcc gcgagccagt cgagaaacgc
     1201 ggtcaccggc acgttgcccg cgatcgagac gtggtggagg agggcgaggc agagcgtcag
     1261 ctccgggcgg ccccgctcct cgagcggcag ccgctcgcgc ccgcgccagc cgagcccggg
     1321 cgacgggtcg gtgacgtcgc cgacgagcgg gaggatccgc gtcgcgccct cggccgcgag
     1381 ctcgcggtag agacggtcga cgaccgccgc atccgcgtcg aaggcgacga cggtgtccgc
     1441 gtgctcggcc gcgatgcgcg cgtggcgccc ctcgttcgcg ccgacgtccc agacgaggcc
     1501 gcgccggcgc cgtgcggcga cctcccggac gaagctctcc ttccgcgcgg catcgtcctc
     1561 cgtgtacgac gtcgtcgcgc cgtagtccga ccaggtcgtc ggcccgggct cccaccgcag
     1621 ccgccggacg agcttctcga gacgccgcac gttcgccgcg acgagctcct tccggaagcc
     1681 cgcccgcgcg agctccgcct tcacgtcgcg ctcgccgtcc ccgtgccggc gctcgaggcg
     1741 cgcgtggagg acgacgtgcg cgagcacccc gggacggagc cggtgcctcc ccgagaggac
     1801 gccgcgcagc tcgtccgggt ggatcccgtc gaggctcccg cgcagccacg gctggaacgg
     1861 ggcgccggtg tacgcctgca ggaggagcgg gttcaggaag aggaggcaga actggcggta
     1921 gccctgccac ggctcgccct cgcgcagccg ctcgaacgag cccacgtcga tgaacacggg
     1981 gctcgtcccg cgccactgga cgttgtacgg cgacgcgtcc ttgagggtca ggtcctcgtc
     2041 gagcgcgcgg cgcacgagct cgagctggag gagcgccgcg tcgcggagca tcgagaacga
     2101 ccactcgtag ggataggaga cgaacgggat ccgttcgtgc tcgaggacgg cggccgcgcc
     2161 ggcctcctcg ggaagccgct cgtccacctc ctcggtcgcg acgagcttgc cctcggcgac
     2221 cgcggccgcg aagagctcgg agccggcgag cgcgcgccac ccgtcgagcc cgtcggggcc
     2281 gagcgcccgg agcacccggc cgtccagtgc gtacacgcga ctgtcccagt cccggaagga
     2341 cccggggtcc ggggtcggcg tgccgggatc cgtgtccggg atccgcaccg ggaggcggcg
     2401 ttacggcgtc gtggtgtccg cgtcgcggcg ctcggccggc cgcggcttgc ggatccggac
     2461 gagctcacgg aagcggtgcc agaacatctt gaagaacacc gcgaccgccg caagtccgcc
     2521 cagcgcgacc tggacgatca tgctgccggt gcccggatcg agatatgcga agcgcatctg
     2581 tttcctcccc cggtttcgtc cctcccggcg gagagggatg gtactcactt ctcggtgacg
     2641 actgcaacaa ggtactcgtg ccgccggccg gcgccgtcag cgcgtagggg tcagccgccg
     2701 agccgggtct cgatctccgc gagcgtcggc tcgcccgccg agcccccgag caggaagacg
     2761 cgcacgtcgt tgtcggcgtc gcggaggaag gcgccgggcg ggacgagcgc ggagaaccgc
     2821 gccgcccccg cctcgcccgt cgccgtcacg accgcctcga cgacgtcgtt caccgcgacc
     2881 gcgatcgcgt cgccgggccg aacgccgccc gtgacggtcc cggcgatcgg gctcgggacg
     2941 agcgccgagc gctccggaag ggaggggagg aggccggcga gccgcgcgga gaggacggcc
     3001 tcgccctcga ccgccgcgct cacgccgaac tcgccgaccg tgagcccgac gagccgcccg
     3061 gacgggcccg gcccgtagag ctcgccgtcc cacgcccccg agccgagcag gtcgacgcgg
     3121 gcggcgagcg ccgcctggcg cgccccgagc gcctccccga agggggcagt caccccgccg
     3181 acgcgcacct cctcggcgcc ccggcgccgc acgagcccgg acgcgccgtc cgtctcccac
     3241 ggaaccggca ccgagagcgc ggccgcgatc gtcggcagga cgtccaccga ggtgacgtgg
     3301 cggtcctcga cccgtcctgt gctctgaccg ggcagcttga ggaagaacgg aacgaacgcg
     3361 atctccgcga gcgtgtcctg cgtgaccgtg ccgccggccg cgtccggccg gaacgcgatg
     3421 ccgtggtcga cgacgacggc gaggagcgcg ttctcccaga ggccgacctc ccgcatccgc
     3481 tcgacgagct gcccgagcag ccggtcggcg tagccggcct ggagaagatg gcgctgccag
     3541 gcctgcagcg cctccgcctc gccgttccgc gcgggtgcct cgcccgggat ggcggactgg
     3601 ctcccgttcg ggaaccgcgt ccagggcccc tgcggcaggc ggaggtggat cacgtcgagc
     3661 gtcggttcct cgccgggctc gtagcgaagc gaccggagga aatcgcggaa gctgcgctcg
     3721 cgctcggcgt cgaagatccc cgtgtcggcg aagctcgacc gctcggggtc cggctcgggc
     3781 aggcggctct cgaggccggg cggcgcgacg acgcgccccc aggtcgcgcc cgcgtcgccg
     3841 aggagcccgc cgacgccggt cccctcccgg ccgtcgcgcc gctcgcagag ctcgggcggg
     3901 cagagacgcg tctgcgtctc ctggacgttc agccggtagt cggccccgag cagcgtgaag
     3961 aggttctccc ggtggttctg gaagatcggc aggcgcccgg gctcggggcg cttccccgtg
     4021 aggagggccg gcacggcctc gtcgccggtc gccgagaccg tggtcgcgtt gcggaaccag
     4081 gtcgacgagc gggcgagcga ggcgaacgcg gggaagcggc cctcgtcgag ctcgccggcc
     4141 gggtcgagga gcgagacggt cgggagctcg ctgaagacga cgacgacgac cggcgtcgcg
     4201 ctccggacgc cggcgatctc gacgcccgtg tcggcgggga gcgcgagctc ggacacgggc
     4261 gagacggcga ggaagagccc gagcgccacg agcgggagcg gcgcgaggaa ggcgagaacg
     4321 gtccccacgg atcgcgcctg cgcgaccgcg agcccggctc ccgccgccgc gagcacgccg
     4381 agcacgacga gcgcgacggt gctctcgacc ccgagccgct cgagccccca gagggcgaag
     4441 agccccgcga gcgcgccgac gagccctacg tgcagccagg cgcccgcctc cgcgttcgcg
     4501 gcccgcacga ggaccacgag cgcggtgagc acgagcggcg gcacgaggac gacggcgagc
     4561 ccgaagagcc agatgtcgag cgccggcgag ccgcgtgcgg cgaagaacgc cgggtcgtcg
     4621 ccgagcgcgg agaggagggg gagcgcgacg ccgagcccgg tcgccgcgag gagctgcgcg
     4681 ccgacccgcc ggaccgacgg ctgcacgggg gtcacgaacg cggggctatc gcgcgtcggg
     4741 ccccggccgc ccgcggcggg cggttccggc gtggatgtct gcgcgattca tgtataggtc
     4801 tccctcggtg ccctggcgcg aggcggcagt atggtacccg gctggtgaga cgaccgtttt
     4861 catgcgacac ggcgggcggg cgacgcccga gaaggagaag agtcacacgt gaagattcgc
     4921 cctgaggaga tcacctcgat cctgaagcag cggatcgagg aatacgacgt cgagacggac
     4981 ctcgcggagg tcgggacggt gctgcagatc ggcgacggtc tcgcgcgcgt ctacgggctc
     5041 gagaactgca tggcgatgga gatgctcgag ctcgagcacg gcgtcgtcgg cgtcgccttc
     5101 aacctcgagg aggacaacgt agggagcccg ctcttcggcg actgggagct gatctccgag
     5161 ggcgcgccgg tcaagcgcac cggcaaggtg gcgagcgtcc cggtcggcga ggcgctcgtc
     5221 gggcggatcg tgaacccgct cggccagccg ctcgacggcc agggcccgat caagacgacg
     5281 gagacgcgcc cgctcgagtt caaggcgccc ggcgtcgtcc agcgccagcc ggtcaccgag
     5341 cccgtccaga ccggcatcaa ggcgatcgac tcgatgatcc cgatcggccg cggccagcgc
     5401 gagctcatca tcggcgaccg ctcgaccggc aagaccgcgc tcctcgtgga cacgatcctg
     5461 aaccagaagg gccaggacat gatctgcgtg tacgtcgcga tcggccagaa ggcctcgacc
     5521 gtcgcgcagg tctacgagcg cctgaaggac gcgggcgcga tggactacac gatcatcgtc
     5581 agcgcgtccg cgaccgaggc ggcgccgatc aagtggatgg cgcccttcgc cgggtgcgcg
     5641 atgggcgagc acttcctcta caacggaaac cacgcgctct gcatgtacga cgacctctcg
     5701 aagcacgcgg acgcgtaccg gcagatgtcg ctgctcctgc ggcgcccgcc ggggcgcgag
     5761 gccttcccgg gcgacgtctt ctacctccac tcgcgcctgc tcgagcgcgc ggtgaagctg
     5821 aacgacgagc tcggcggcgg gtcgctcacc gcgctccccg tgatcgagac gcaggcgggc
     5881 gacgtgtccg cgtacatccc gacgaacgtc atctcgatca ccgacgggca gatcttcctc
     5941 gagagctcgc tcttctactc cggcgtgcgc ccggcgatca acgtcgggat ctcggtctcc
     6001 cgcgtgggcg ggaacgcgca gacgaaggcg atgaagaagg tcgccggccg cctgcggctc
     6061 gacctcgccc agtaccgcga gctcgaggcc ttcgcgcagt tcgggtcgga gctcgacccg
     6121 cagacgcagc gcaccctcgc gcgcggcgag cggatggtcg cgacgctgaa ccagccgcag
     6181 tacgcgccct ggccgctcga ggagcaggtc gccgcgatct tcgcggggat caacggctat
     6241 ctcgacgacg tcccgccggg ccaggtcccg cgcttccagg aggagctgcg cgagcacctg
     6301 cgcaccgagg ggtcggtcta caaggacatc cgcgagggca aggagctccc ggacgagctc
     6361 gccgaccggc tcaaggccga gatcgacaag ttcaagggga acttcgcggt ccgcgaggag
     6421 agcgcggtcc cgggaacgta gatggcgacc gtccaggaca tcaagcgccg ggtcagctcg
     6481 gtcaagaaca cgagcaagat cacgaaggcg atggagctcg tcgccgcggc gaagctccgc
     6541 aaggccgagg cgcggatcga ggcgctccgc ccgtacgccg accggatgcg cgagctcatg
     6601 atcggcaccg cgcgggcgac gccgtcgcgg ggcttcccgc tcctcgagga gcgagagaac
     6661 gtgagctcgg tcgccatcct ctcgctcacg ggcgaccgcg gtctcgcggg cgggttcaac
     6721 gcgcagatca tccgccgctc gctcgcgctc gagcggcagg tccgcggcga cggcgccgac
     6781 gtgcgctggc tcgtcgtcgg gaagaagggc cgctcgacgc tccgcttccg ccgctacgac
     6841 gtcgcgcacg cctgggtcga cctgaccgac cggccgacgt acgccgatgc ggccgcggtg
     6901 gcgaacgccg tcgccgagct ctacaccgag cgcgaggtcg accgggtcgt catggtctac
     6961 aaccgctacc tctccgcgct cacgcagcgg gtcgaggtgg aggaggtgct cccgatcccg
     7021 cgcaaggcgc tcgaggagga cgaggaccgg agcgcgtacg agatcgcgct cgagggcgac
     7081 ttcatctacg agccggagcc ggacgtcatc ctcggccggc tgctcccgac ctacctcgag
     7141 acctcgatct accgcgcgct cctcgagtcc gc
//
LOCUS       DASTFC010000307         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_41229,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000307 DASTFC010000000
VERSION     DASTFC010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41229"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFO88_09095"
     CDS             complement(<1..1472)
                     /locus_tag="VFO88_09095"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9545723.1"
                     /translation="MRPALTLAVAAAVFWVGFDAGSYSLESRAILAIVVLWGTMLAFV
                     LGLWPLRRPPLAARVAGGLLGAFALFTGLSVFWAESAERALVEFNRAVLLVAVFAVAV
                     LAGTRGNVGRWLDGIALGIAAIGVLALAGRLFPDVFPTSAVQDFLPAVASRLSWPVEY
                     WNGLAMLVGLALPLLLRVATSAHPVARGAAVGAVPALAGTLFLSSSRGGFAVAFVGVA
                     AFFVLGPRRWGTAAAGVLAAAGSVAAVAILHARDELVNMPTAPEAVGQGRSAALLILV
                     VCLATGALYAAGSHLFAERRTPPLPGWAAAVAVLVVAVVAAVAVDAAERFGEFCDPPP
                     EFARDDFVQAHLVSGTGSGRCQFWGAALDQWREAPLAGQGAGSYEAWWSQNGTIPYFI
                     RDAHSLYLETLGELGVIGFLLLAGALATGLVAGARRLLAAADADEAVAIAAVLSGFLA
                     FLASAAIDWMWEETVVAVVGLLLLGLAVGPATSTAAPALAP"
     gene            1509..2060
                     /locus_tag="VFO88_09100"
     CDS             1509..2060
                     /locus_tag="VFO88_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545724.1"
                     /translation="MRSVFGVELMRRSVRRRVLAFAAIVGATLLATSAPAHAQLSGGP
                     GAVDQYVEDVPTGGGTSHPTADPPSSTPAPSGVQQQIDAQGGSDAPLLTQVVSSSTYG
                     APQGRAVNKGGGSSAERMERGGELPEAVSSGTESSGVVSSAVTAVEGSDARRLLGLLL
                     ALLAVTAATVFAVGLRQRRANRG"
     gene            complement(2123..2213)
                     /locus_tag="VFO88_09105"
     tRNA            complement(2123..2213)
                     /locus_tag="VFO88_09105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2177..2179),aa:Ser,seq:gct)
BASE COUNT          341 a          875 c          921 g          251 t
ORIGIN      
        1 ggggcgagcg ccggcgcggc ggtgctcgtg gccgggccga cggcgaggcc gagcaggagc
       61 aggccgacga cggcgacgac cgtctcctcc cacatccagt cgatcgcggc ggaggccagg
      121 aaggcgagga agcccgagag gacggcggcg atcgcgacgg cctcgtccgc gtcggccgcg
      181 gcgagcagcc gccgcgcgcc ggcgacgagg ccggtggcga gcgcgcccgc gaggagcagg
      241 aatccgatca cgccgagctc gccgagggtc tcgaggtaga gcgagtgcgc gtcgcggatg
      301 aagtacggga tcgtcccgtt ctgcgaccac cacgcctcgt acgacccggc gccctggccg
      361 gcgagcggcg cctcgcgcca ctggtcgagc gcggcgcccc agaactggca gcggccgctt
      421 cccgtcccgc tcacgagatg ggcctggacg aagtcgtcgc gcgcgaactc gggcggcggg
      481 tcgcagaact cgccgaaccg ctccgccgcg tcgaccgcga cggcggcgac gacggccacg
      541 acgagcacgg cgaccgcggc cgcccagccg gggagcggcg gcgtgcggcg ttccgcgaag
      601 aggtgcgagc cggccgcata gagcgcgccg gtggcgaggc agacgacgag gatgaggagc
      661 gcggcgctgc gtccctggcc gaccgcctcg ggcgccgtcg gcatgttcac cagctcgtcg
      721 cgcgcgtgga ggatcgcgac cgccgcgacc gacccggccg ccgcgaggac gcccgccgcg
      781 gcggttcccc agcggcgcgg gccgagcacg aagaacgcgg ccacgccgac gaacgcgacc
      841 gcgaagccgc cgcgcgacga gctcaggaag agcgtccccg cgagcgcggg cacggcgccg
      901 accgccgccc cgcgcgcgac cgggtgcgcg ctcgtcgcca cgcggaggag cagcggcagc
      961 gcgagcccga cgagcatggc gagcccgttc cagtactcga ccggccagct cagtcggctc
     1021 gcgaccgcgg ggaggaagtc ctgcacggcg ctcgtcggga agacgtcggg gaagagccgg
     1081 ccggcgagcg cgaggacgcc gatcgcggcg atcccgaggg cgatcccgtc gagccagcgc
     1141 ccgacgttcc cgcgcgtgcc ggcgaggacg gcgaccgcga agacggcgac gaggagcacg
     1201 gcgcggttga actcgacgag cgcgcgctcg gcgctctcgg cccagaagac ggagagcccc
     1261 gtgaagagcg cgaatgcgcc gaggaggcct ccggcgacgc gcgcggcgag cggcgggcgc
     1321 cgaagcggcc agaggccgag cacgaacgcg agcatcgttc cccagaggac gacgatcgcg
     1381 aggatcgcgc ggctctcgag cgagtaggag cccgcgtcga acccgaccca gaagacggcc
     1441 gcggccacag cgagcgtgag tgcgggtcgc atgtttctca tttgacagcc ggggcaggcg
     1501 tcacgaccat gaggtcagtg ttcggggtcg agctcatgcg gcggagcgtc cggcgacgcg
     1561 tgctcgcgtt cgcggcgatt gtcggcgcca ccctactggc aacctcggcg cccgcccacg
     1621 cgcagctgtc gggcggcccg ggtgcggtcg accagtacgt cgaggacgtt ccgacgggcg
     1681 gcggcacgtc gcacccgacg gccgacccgc cgagctccac gcccgcgccg agcggcgtgc
     1741 agcagcagat cgacgcgcag ggcggctccg atgcgccgct cctgacgcag gtcgtctcgt
     1801 cgtcgaccta cggcgctccg caggggcgcg ccgtcaacaa gggcggcggc tcgtcggccg
     1861 agcggatgga gcgcggcggc gagctccccg aggccgtgtc gtccggcacc gagtcgtccg
     1921 gggtcgtctc ctccgccgtc acggcggtcg agggcagcga cgcccggcgc ctgctcggcc
     1981 tgctcctcgc gctcctggcc gtgacggccg ccaccgtgtt cgcggtcggc ctgcggcagc
     2041 gccgcgcgaa ccgcggctag cgcgtccgcc tcttcacgcg gcgctccgcc gttcgcggcg
     2101 cgccgtcgtc cacctcgtga ggcggagaag gagggattcg aaccctcgag ggaagcttgt
     2161 gcccccctaa cgccttagca gggcgccgcc ttcagccact cggccacttc tccgtgcggc
     2221 catcgtacca acgtcgatcg gcggcttccg cggcccgcgc ccggcgctcc cgccgcttaa
     2281 aggacgaagc ccgcctcggc gagccatggg gagccgcttg atcgtgacgg gcgtcgtcgg
     2341 tgccgggtta tgaggggagc cggcgctctc atcatgtggc ggctgccg
//
LOCUS       DASTFC010000308         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_650788,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000308 DASTFC010000000
VERSION     DASTFC010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650788"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFO88_09110"
     CDS             <1..729
                     /locus_tag="VFO88_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9545725.1"
                     /translation="PGVNLVFAVALLAVVFALGVPVANTVESVVAGSPAERAGLEPGD
                     RIVEVNGNEITPILTERIPARITGTEARDVAVVVERDGERVALSPTRTEEIDGAYRLG
                     FVLARTEESFGPLESTEYALRETWAITTATGSALARIVTGSGRDEISSPVGIVQVSSE
                     SIGIDYRIYLRLLAIISLSLALLNLLPLLPLDGGHIVFSLAEALRGRAIPRVAYERAS
                     MIGIFLVLMLFALGLSNDIGRLSG"
     gene            726..1244
                     /locus_tag="VFO88_09115"
     CDS             726..1244
                     /locus_tag="VFO88_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET9545726.1"
                     /translation="MTRRRVAAGAAVLLAVLAVLTARWILWPATDEPGRADAVVVLGG
                     GLGERLPAGRELVERGVAPVLVVSHGSRGCGTRRDGYEIVCFTPDPDRTQGEARAAAR
                     LARERGWETLVVVTSTYHAARATKLFERCVDGEVRGVGVDPPTSGGLPTPSNVLREWV
                     AWGHALVAERGC"
     gene            1284..2408
                     /gene="ispG"
                     /locus_tag="VFO88_09120"
                     /gene_synonym="gcpE"
     CDS             1284..2408
                     /gene="ispG"
                     /locus_tag="VFO88_09120"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET9545727.1"
                     /translation="MPSELQIKVGNVPVGGGAPVAVQSMTTTATHDVDATMAQIGALA
                     SAGCEIVRVAVPKNEDAAALAKLVYLSPLPVIADIHFNASLALKAIEAGVAAVRINPG
                     NIGGPDKVEQVVRAAKARGIPMRIGANSGSLPEHLKELAQKDTAEALVVEALEQVELL
                     ERLDYRDFKISVKSSSVPTMIRAYRMLADRVPYPLHLGVTEAGTPFAGSVKSAVGLGS
                     LLMDGIGDTVRVSLTADPVEEVKVAWEILKALGLRERGPVMIACPSCGRDNVGVHLLA
                     EAVEERLTAYPQAFEVAVMGCAVNGPGEAGDADFGIAGGRETGFVYAHGRVLKKVESE
                     ILVDELFREIDRWIADGMVRPKRLKLAKPAALMMAEAAQR"
     gene            2408..4090
                     /locus_tag="VFO88_09125"
     CDS             2408..4090
                     /locus_tag="VFO88_09125"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9545728.1"
                     /translation="MARLSQLFVPTLREDPADAEAASHKLLVRGGYIRQVGAGLWSFL
                     PLGWRIHEKVVAIVREEMNAIGGQEMFMPVVTPAELWQATSRYGIPEVFKLEDRVGRP
                     LVLAMTHEETVTFHARELGSYRDLPQILYHFQTKGRDEPRPRGGLLRVREFVMKDSYS
                     FDRDEEGLEESFRAHEGAYHRIFERCGIEFQAVQAESGMMGGSESVDFLAPAGAGENT
                     LVTCERGDYAADLEIARGVPRAPELPPRLDAPEAIDTPGITTIEALAGSLGIDEAATS
                     KAMPVVNGDGEVVLALVRGDDRLDESKLAAVLGSAVRQATEAEIRSAFGADPGSIGPV
                     GFSGEVVADETLREGQFVAGANETGRHLRGVEHGRDFEARFADLRVPKEGDACPRCGG
                     ALRFQTAIEVGHIFKLGTRYSEPLGARFLDADGVERPVVMGSYGIGPGRVVSAAAEQG
                     ADEQGVAWPRSIAPYDVEVVPIEAAGPDAMGIAERLVDDLEGAGLDVLVDDRDRRPGE
                     KFADADLIGCPVRITVGKKVLEDGRVDVLTRAGRHEERVQVSDVINRMTEGA"
     gene            4090..4389
                     /locus_tag="VFO88_09130"
     CDS             4090..4389
                     /locus_tag="VFO88_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9545729.1"
                     /translation="MARRRRFSEEPFGPTVQGLMAETGVTYRGLAERAGLSAGYLNHL
                     VHGNRPVPSNDVMATLARALGVEPEHFREYRIRVITEKLEAMPDLVDRLYKRYAE"
     gene            4397..4525
                     /locus_tag="VFO88_09135"
     CDS             4397..4525
                     /locus_tag="VFO88_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545730.1"
                     /translation="MGDWTDANVHDPGITMLQALAWSIAGLLAAGLVWRFLSRRDP"
     gene            4522..4866
                     /locus_tag="VFO88_09140"
     CDS             4522..4866
                     /locus_tag="VFO88_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545731.1"
                     /translation="MSADAVRAALLELHREVLRAERVTLERIHGRMGAAEFLQVAADD
                     PKLAWLVPLSELVVAFDEEDADEEALVARARALLSPPDPATPFGRRYLSVLQRSPEVV
                     LAHQAGTDMLPD"
BASE COUNT          685 a         1744 c         1874 g          678 t
ORIGIN      
        1 ccgggcgtga acctcgtctt cgccgtcgcg ctgctcgccg tcgtcttcgc gctcggcgtc
       61 ccggtcgcga acacggtcga gagcgtcgtc gccggctcgc ccgccgagcg tgccggtctc
      121 gagcccggcg accgcatcgt cgaggtaaac gggaacgaga tcaccccgat cctcaccgag
      181 cggatccccg cgcggatcac cggcaccgag gcgcgggacg tcgccgtcgt ggtcgagcgt
      241 gacggtgagc gggtggcgct ttcaccgacg cgcacggagg agatcgacgg ggcgtaccgc
      301 ctcggcttcg tgctcgcgcg cacggaggag agcttcgggc cgctcgagtc gacggagtac
      361 gcgctccgcg agacgtgggc gatcacgacc gcgaccgggt ccgcgctcgc gcggatcgtg
      421 accggctccg gccgcgacga gatctcgagc cccgtcggga tcgtgcaggt ctcgagcgag
      481 tcgatcggga tcgactaccg gatctacctg cgcctgctcg cgatcatcag cctctcgctc
      541 gcgctgctga acctcctgcc cctcctgccg ctcgacggcg gccacatcgt gttctcgctc
      601 gcggaggcgc tgcgcggccg ggcgatcccg cgcgtcgcgt acgagcgggc gtcgatgatc
      661 gggatcttcc tcgtgctcat gctcttcgcg ctcggcctct cgaacgacat aggccgcctc
      721 agcggttgac ccgccgccgg gtggcggccg gcgccgccgt gctgctcgcg gtgctcgccg
      781 ttctcacggc gcgctggatc ctctggccgg cgacggacga gcccggccga gccgacgccg
      841 tcgtcgtcct cgggggcggc ctgggggagc ggctccccgc ggggcgcgag ctcgtggagc
      901 ggggcgtcgc tcccgtcctc gtcgtctcgc acggctcccg cgggtgcggc acgcgccgcg
      961 acggctacga gatcgtctgc ttcacgcccg acccggaccg cacgcagggc gaggcccgcg
     1021 ccgccgcccg cctcgcccgc gagcgcggct gggagacgct cgtcgtcgtc acgtcgacgt
     1081 accacgccgc gcgcgcgacg aagctcttcg agcgctgcgt cgacggcgag gtgcgcgggg
     1141 tgggcgtcga cccgcccacg agcggcggcc tcccgacccc gtcgaacgtc ctccgcgagt
     1201 gggtcgcctg gggccacgcg ctcgtcgcgg agcgcgggtg ctagcgaggc ccggcaaacc
     1261 cgcacggcta cactgatcgc cgcatgccga gcgagcttca gatcaaggtc ggcaacgtcc
     1321 cggtcggagg cggcgcgccc gtcgcggtcc agtccatgac gaccaccgcg acccacgacg
     1381 tcgacgcgac gatggcgcag atcggcgcgc tcgcctcggc gggctgcgag atcgtcaggg
     1441 tggcggtgcc gaagaacgag gacgccgcgg cgctcgcgaa gctcgtctac ctgagcccgc
     1501 tccccgtcat cgcggacatc cacttcaacg cctcgctcgc gctcaaggcg atcgaggccg
     1561 gcgtggcggc cgtgcggatc aaccccggca acatcggcgg ccccgacaag gtcgagcagg
     1621 tcgtccgcgc cgcgaaggcg cgcgggatcc cgatgcgcat cggcgcgaac tccggctcgc
     1681 tccccgagca cctgaaggag ctggcgcaga aggacacggc cgaggcgctc gtcgtcgagg
     1741 cgctcgagca ggtcgagctg ctcgagaggc tcgactaccg ggacttcaag atctccgtca
     1801 agtcgagctc ggtgccgacg atgatccgcg cgtaccggat gctcgccgac cgcgtgccgt
     1861 acccgctcca cctcggcgtg accgaggcgg ggacgccgtt cgccggctcg gtcaagagcg
     1921 cggtcgggct cggctcgctc ctcatggacg ggatcggcga caccgtgcgc gtgagcctga
     1981 ccgccgaccc ggtcgaggag gtcaaggtcg cgtgggagat cctgaaggcg ctcggcctgc
     2041 gcgagcgcgg gcccgtgatg atcgcctgcc cgagctgcgg gcgcgacaac gtcggcgtgc
     2101 acctgctcgc cgaggcggtc gaggagcggc tcacggcgta cccgcaggcc ttcgaggtgg
     2161 ccgtcatggg ctgcgccgtg aacggcccgg gcgaggcggg cgacgcggac ttcgggatcg
     2221 cgggcggccg cgagaccggc ttcgtctacg cgcacggccg cgtgctgaag aaggtcgagt
     2281 ccgagatcct cgtcgacgag ctcttccgcg agatcgaccg ctggatcgcc gacgggatgg
     2341 tccggccgaa gcgcctgaag ctggccaagc cggccgcgct catgatggcg gaagccgcac
     2401 aacgctgatg gcccgactct cgcagctctt cgtcccgacg ctacgggaag acccggccga
     2461 tgccgaggcc gcgagccaca agctcctcgt gcggggcggc tacatccgcc aggtgggggc
     2521 ggggctctgg agcttcctcc cgctcggctg gcggatccac gagaaggtcg tggcgatcgt
     2581 ccgcgaggag atgaacgcga tcggcggcca ggagatgttc atgccggtcg tgacgcccgc
     2641 ggagctctgg caggcgacga gccgctacgg gatcccggag gttttcaagc tcgaggaccg
     2701 cgtcgggcgc ccgctcgtgc tcgcgatgac gcacgaggag acggtcacgt tccacgcgcg
     2761 cgagctcggc tcgtaccgcg acctcccgca gatcctctac cacttccaga cgaagggccg
     2821 cgacgagccg cggccgcgcg gcgggctgct ccgcgtgcgc gagttcgtca tgaaggactc
     2881 gtattcgttc gaccgcgacg aggaggggct cgaggagagc ttccgcgcgc acgagggggc
     2941 gtaccaccgg atcttcgagc gctgcgggat cgagttccag gccgtccagg ccgagagcgg
     3001 gatgatgggg gggagcgagt cggtcgactt cctcgcgccg gccggggcgg gcgagaacac
     3061 gctcgtcacg tgcgagcggg gcgactacgc ggccgacctc gagatcgcac gcggcgtgcc
     3121 gcgcgcgccg gagctcccgc cgcggctcga cgcgcccgag gcgatcgaca cgccggggat
     3181 cacgacgatc gaggcgctcg ccgggtcgct cgggatcgac gaggcggcga cgtcgaaggc
     3241 gatgcccgtc gtgaacggcg acggcgaggt cgtcctcgcg ctcgtgcgcg gcgacgatcg
     3301 cctcgacgag tcgaagctcg ccgccgtgct cggctctgcg gtgcggcagg cgaccgaggc
     3361 ggagatccgg tcggccttcg gcgccgatcc cggctcgatc gggccggtcg ggttctccgg
     3421 ggaggtcgtc gcggacgaga cgcttcgcga gggccagttc gtcgcgggcg cgaacgagac
     3481 cggccggcac ctgcgcggcg tcgagcacgg gcgcgacttc gaggcgcgct tcgccgacct
     3541 tcgcgtgccg aaggagggcg acgcgtgccc gcgctgcggg ggtgcgctgc ggttccagac
     3601 ggcgatcgag gtcggccaca tcttcaagct cggcacccgc tactccgagc cgctcggcgc
     3661 gcggttccta gacgcggacg gcgtcgagcg gccggtcgtc atgggctcgt acgggatcgg
     3721 gcccgggcgc gtcgtgagcg ccgcggcgga gcagggagcg gacgagcagg gcgtcgcctg
     3781 gccgcgctcg atcgcgccgt acgacgtcga ggtcgtgccg atcgaggcgg ccggtcccga
     3841 cgcgatgggc atcgccgagc ggctcgtcga cgatctcgaa ggcgcaggcc tcgacgtgct
     3901 cgtggacgac cgcgaccggc ggccgggcga gaagttcgcg gacgccgacc tcatcgggtg
     3961 ccccgtccgc atcacggtcg gcaagaaggt gctggaggac ggcagggtcg acgtgctgac
     4021 gcgcgccggc agacacgagg aaagggtgca ggtgagtgac gtgatcaatc ggatgacgga
     4081 gggtgcgtga tggcgcggag gcggcgtttc agcgaggagc cgttcgggcc gacggtccag
     4141 ggcctcatgg cggagaccgg cgtgacgtac cgcgggctcg cggagcgcgc ggggctctcg
     4201 gccgggtacc tgaaccacct ggtgcacggg aaccggccgg ttccgtcgaa cgacgtgatg
     4261 gcgacactcg cgcgagcgct cggggtcgag cccgagcact tccgcgagta ccgcatccgc
     4321 gtgatcaccg agaagctcga ggcgatgccg gatctcgtcg atcggctcta caagcgctac
     4381 gcggagtaag agggccgtgg gcgactggac ggacgcgaac gtccacgacc cggggatcac
     4441 gatgctccag gcgctcgcct ggagcatcgc cgggctcctc gcggcggggc tcgtgtggcg
     4501 cttccttagc cggcgcgacc cgtgagcgcg gacgccgtcc gggcggcgct cctcgagctg
     4561 caccgggagg tcctgcgggc cgagcgcgtg acgctcgagc ggatccacgg ccgcatgggc
     4621 gccgcggagt ttctccaggt ggcggcggac gacccgaagc tcgcgtggct cgtcccgctc
     4681 tcggagctcg tcgtcgcgtt cgacgaggag gacgcggacg aggaggccct cgtcgcccgc
     4741 gcgcgtgcgc tgctgtctcc gcccgatccc gcgaccccgt tcggccggcg ttacctctcc
     4801 gtgctgcagc ggagccccga agtcgtcctc gcccatcagg cagggaccga catgctgccg
     4861 gactgagcgc tcccgcaacg cgcggctcgg cgcgccgccc catgggcgcg ttgagatgcg
     4921 taaacgcggg ggtcttgcca tcggagaggc gtccggtaac aatggcgccc cgaaagcccg
     4981 a
//
LOCUS       DASTFC010000309         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_420200,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000309 DASTFC010000000
VERSION     DASTFC010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420200"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1047
                     /locus_tag="VFO88_09145"
     CDS             154..1047
                     /locus_tag="VFO88_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9545732.1"
                     /translation="MNELLQLIANGLVTGSIIAITAVGLSLVYGILKIVNFAHGEYLV
                     FGAYMALVVNVTWGQSLILGALFALVMTALLGIALEFGLWRPIRKRGAGIVSLLIVSI
                     GLALVLRNSILLWQGGAPRRYDVDVFQVYELGPIRLSLSQIVVIVVASVVIIGVGLLL
                     AQTTLGKAMRALSDSRDLASVSGIDTDRITAYTWIVGSALAGLGGVLQGLVQNAFEPN
                     MGFRLLLLIFAAVILGGVGSAYGALVGGLVLGLMMEVSTWSELPAGGVDSVYKPVVAF
                     VVLILVLIFRPQGIFGRARTV"
     gene            1044..2024
                     /locus_tag="VFO88_09150"
     CDS             1044..2024
                     /locus_tag="VFO88_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9545733.1"
                     /translation="MSGLANVQFWAFVGVVAGIYTIFALGLQLQFGFTGLLNFGHVAF
                     MAIGAYTMAILVVKVEWPLWLSALVAIAVSMLFGLIIGLPTVRLRADYLAITTIAFGE
                     IIRFIAVNQQTLTGGPQGTINLTGAGRASSYNREWLDFQAWAQDQLEVILGDHATRDV
                     TMLVLVWIVALVLIVLLQFMVHSPWGRVLRSVREDEDATSALGKNVFSYKLQALAVGA
                     AIGAIAGLFFAFQFSFFSPQDFDPLTTFFAYVIVLLGGTAKNWGVPVGALIFAFIFAG
                     TRFLDFWPLSELTSADRAFLRLIAVGLILIGLMAFRPQGIFGKKEEMVLE"
     gene            2021..2866
                     /locus_tag="VFO88_09155"
     CDS             2021..2866
                     /locus_tag="VFO88_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9545734.1"
                     /translation="MSAPERNGTILELSDVVKHFGGVAAVAGAELDVRRGSITALIGP
                     NGAGKTTLFNIVSGFYKPERGVITFEGERIDRKPPHAIAKRGLVRTFQITKALTKMSV
                     LENMMLAAPDQPGEQLWRLVFTPRSARGREREVHEKANELLKLVRLDRLADDYAGTLS
                     GGQRKLLEFGRALMRDPTMIMLDEPMAGVNPSLGLELLDHMKRLQEERGTTFLLIEHD
                     MEVVMNVSERVIVMNEGRVISAGPPDEVRQDQAVIDAYLGTGRGAGESAPPAEGTPPS
                     EGDTA"
     gene            2863..3699
                     /locus_tag="VFO88_09160"
     CDS             2863..3699
                     /locus_tag="VFO88_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9545735.1"
                     /translation="MSTDAPLLETADLDAGYGEVNILHGLSISVPRGAIVSVIGPNGA
                     GKSTLLKAIYGFLKPRRGTVTFRPDGAEHDITGLKPNKLTELGMNYVFQLDNVFPNMT
                     VRENLEMGAYLRGGKPDKEILERIHGWFPRLDERRRQRAGTLSGGERQMLALGRALMS
                     DPKLLLLDEPSAGLAPSVVDVIFEKLQEINRAGISIVMVEQNARRSLEMSDYGYVLDM
                     GRNHFEGAGSELLHDESVVELYLGGRGRLAAAQLIADEDYEASREALEERHGEFGHEK
                     DA"
     gene            complement(3853..>4448)
                     /locus_tag="VFO88_09165"
     CDS             complement(3853..>4448)
                     /locus_tag="VFO88_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545736.1"
                     /translation="GEPDAWLIIDFPETFAKVGPALVRAGGWDPTRTFTADGLRSSDL
                     PGDVGEEATEGMRGTAPTSEGAPAGDAFATAYEERAEGVSRQTFDAQAFDAVIVSFLA
                     AVRGGASDSETIRDNLQAVSGAGEGEGADQYTFEQLGDAINALLAGEEIDYEGASGPI
                     NFDENGDPGAALYEVWQFEGNEISTLDSFEFSGTDEG"
BASE COUNT          651 a         1607 c         1475 g          715 t
ORIGIN      
        1 gcgagctggg agcgagcctc cgatgaccgt gcgaacgcct cccagataca tgacgccacc
       61 catggcgcgc gcgggcgcgg cccgttacct ctctaagccc atgcggcgcc gcctgccttc
      121 gcacggacgc tctcccgccg aaacgacgct taggtgaacg agctcctcca gctcatcgcg
      181 aacggcctcg tgaccggatc gatcatcgcg atcacggcgg tcggcctctc gctcgtctac
      241 gggatcctca agatcgtcaa cttcgcgcac ggcgagtacc tcgtcttcgg cgcgtacatg
      301 gcgctcgtcg tcaacgtgac gtgggggcag agcctcatcc tgggcgcgct cttcgcgctg
      361 gtcatgacgg cgctcctcgg gatcgcgctc gagttcgggc tctggcgccc gatccggaag
      421 aggggcgccg ggatcgtgag cctgctcatc gtctcgatcg gcctcgcgct cgtcctccgg
      481 aactcgatcc tgctctggca gggcggcgcc ccgcggcgct acgacgtcga tgtcttccag
      541 gtctacgagc tcggcccgat ccgcctctcg ctgagccaga tcgtcgtcat cgtcgtcgcg
      601 agcgtggtga tcatcggggt cggcctgctc ctcgcgcaga cgacgctcgg aaaggccatg
      661 cgcgcgctct cggacagccg cgacctcgcg tcggtctccg ggatcgacac cgatcggatc
      721 accgcgtaca cgtggatcgt cggctcggcg ctcgccggcc tcggcggcgt cctccagggc
      781 ctcgtccaga acgcgttcga gccgaacatg ggcttccggc tcctcctgct catcttcgcc
      841 gccgtcatcc tcggcggcgt cggcagcgcg tacggcgcgc tcgtcggcgg cctcgtcctc
      901 gggctcatga tggaggtgtc gacctggagc gagctccccg cgggcggcgt cgactccgtc
      961 tacaagccgg tcgtcgcgtt cgtcgtcctg atcctcgtcc tcatcttccg cccgcagggc
     1021 atcttcggaa gggcgcgcac cgtatgagcg ggctcgcaaa cgtccagttc tgggccttcg
     1081 tcggcgtcgt ggccggcatc tacacgatct tcgcgctcgg cctgcagctt cagttcggct
     1141 tcacgggcct gctcaacttc gggcacgtcg cgttcatggc gatcggcgcg tacacgatgg
     1201 ccatcctcgt cgtgaaggtc gagtggccgc tctggctctc ggcgctcgtc gccatcgcgg
     1261 tctcgatgct cttcggcctc atcatcggcc tgccgacggt ccggctcaga gccgactacc
     1321 tggcgatcac gacgatcgcc ttcggggaga tcatcagatt catcgcggtg aaccagcaga
     1381 cgctgaccgg cggcccgcag ggcacgatca acctgaccgg cgccgggcgg gcgtcgtcgt
     1441 acaaccgcga gtggctcgac ttccaggcgt gggcgcagga tcagctcgag gtgatcctcg
     1501 gcgaccacgc aacccgcgac gtgacgatgc tcgtcctggt ctggatcgtc gccctcgtgc
     1561 tgatcgtgct gctccagttc atggtgcact ccccgtgggg ccgggttctc cgctccgtgc
     1621 gcgaggacga ggacgcgacc tccgcgctcg ggaagaacgt cttctcgtac aagctccagg
     1681 cactcgcggt cggcgccgca atcggcgcga tcgccggcct cttcttcgcc ttccagttct
     1741 ccttcttcag cccgcaggac ttcgacccgc tgacgacctt cttcgcgtac gtgatcgtgc
     1801 tgctcggcgg gacggcgaag aactggggcg tccccgtcgg cgcgctgatc ttcgccttca
     1861 tctttgcggg gacacgcttc ctcgacttct ggccgctgag cgagctcacg tcggccgacc
     1921 gcgcgttcct gcgcctgatc gccgtcggcc tgatcctcat cggcctcatg gccttccgcc
     1981 cgcagggcat cttcgggaag aaggaggaga tggtgctcga atgagcgcgc ccgagcgcaa
     2041 cggcacgatc ctcgagctct ccgacgtggt caagcacttc ggcggcgtcg ccgcggtcgc
     2101 gggagccgag ctcgacgtcc gccgcggctc gatcacggcg ctcatcgggc cgaacggggc
     2161 cggcaagacg acgctcttca acatcgtctc gggcttctac aagccggagc gcggcgtcat
     2221 cacgttcgag ggcgagcgga tcgaccgcaa gccgccgcac gcgatcgcga agcgcggcct
     2281 cgtccggacg ttccagatca cgaaggcgct cacgaagatg agcgtcctcg agaacatgat
     2341 gctcgcggcg ccggaccagc ccggcgagca gctctggcgc ctcgtcttca ccccccgctc
     2401 cgcccgcggg cgcgagcggg aggtgcacga gaaggccaac gagctcctga agctcgtccg
     2461 cctcgaccgg ctcgcggacg actacgcggg cacgctctcc ggcggccagc gcaagctgct
     2521 cgagttcggg cgcgcgctca tgcgcgaccc gacgatgatc atgctcgacg agccgatggc
     2581 gggcgttaac ccgtcgctcg ggctcgagct cctcgatcac atgaagcgtc tgcaggagga
     2641 gcgcggcacg accttcctcc tcatcgagca cgatatggag gtcgtcatga acgtcagcga
     2701 gcgcgtgatc gtcatgaacg agggccgcgt gatctcggcc ggcccgccgg acgaggtccg
     2761 ccaggaccag gccgtcatcg acgcgtacct cggcaccggc cgcggcgcgg gtgagtcggc
     2821 cccgccggcc gaggggacgc cgccgagcga gggggacacc gcatgagcac cgacgcgccg
     2881 cttctcgaga ccgccgacct cgacgccggc tacggcgagg tcaacatcct ccacgggctc
     2941 tcgatctccg tcccgcgcgg ggcgatcgta agcgtgatcg ggccgaacgg cgccgggaag
     3001 tcgacgctcc tcaaggcgat ctacggcttc ctcaagccgc gccgcggcac cgtgaccttc
     3061 cgccccgacg gcgcggagca cgacatcacg ggcctgaagc cgaacaagct gaccgagctc
     3121 gggatgaact acgtgttcca gctcgacaac gtcttcccga acatgaccgt gcgggagaac
     3181 ctcgagatgg gcgcgtacct gcgcggcggc aagccggaca aggagatcct cgaacggatc
     3241 cacggctggt tcccacggct cgacgagcgc cggcgccagc gcgcggggac gctttccggc
     3301 ggcgagcggc agatgctcgc gctcggccgc gcgctcatga gcgacccgaa gctcctcctc
     3361 ctggacgagc cgtcggccgg cctcgcgccg tccgtcgtcg acgtgatctt cgagaagctc
     3421 caggagatca accgggcagg gatctcgatc gtcatggtcg agcagaacgc ccgacgctcg
     3481 ctcgagatga gcgactacgg ctacgtgctc gacatgggcc ggaaccactt cgagggcgcc
     3541 ggctcggagc tcctccacga cgagtcggtc gtcgagctct acctcggcgg ccgcggccgc
     3601 ctcgccgccg cgcagctcat cgccgacgag gactacgagg cgagccgcga ggcgctcgag
     3661 gagcgccacg gggagttcgg ccacgagaag gacgcctgag cgccgcccga cgccgcgtcc
     3721 gccgcacgag agccgaacga gcaccgaaac agagagggcg agccgggtgt cccggcccgc
     3781 cctcgtcctc ccggagcggt ggaggggcag gacctgcgtc ccgcccctcc gtggaggcag
     3841 cttcgtgggc ggctagccct cgtcggtgcc gctgaactcg aagctgtcga gggtcgagat
     3901 ctcgttgccc tcgaactgcc acacctcgta gagcgccgca cccggatcgc cgttctcgtc
     3961 gaagttgatc gggccggatg cgccctcgta gtcgatctcc tcgcctgcca ggagcgcgtt
     4021 gatcgcgtcg ccgagctgct cgaaggtgta ctgatcggca ccctcgccct cgcctgcgcc
     4081 gctcacggcc tggaggttgt cgcggatcgt ctccgagtcg gacgcgccgc cgcggacggc
     4141 agcaaggaac gacacgatga ctgcgtcgaa cgcctgcgcg tcgaacgtct gacgcgagac
     4201 gccctcggcc cgctcctcgt acgcggtcgc gaacgcgtcg ccggcgggtg cgccctcgga
     4261 cgtcggggcg gtgccccgca tgccctcggt tgcctcctcg ccgacgtcgc ccggcaggtc
     4321 gctcgagcgg agaccgtccg ccgtgaacgt ccgcgtcgga tcccagccgc ccgcgcgcac
     4381 gagcgccggg ccgaccttcg cgaacgtctc ggggaagtcg atgatgagcc acgcgtcggg
     4441 ctcgccgc
//
LOCUS       DASTFC010000310         7872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_337663,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000310 DASTFC010000000
VERSION     DASTFC010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337663"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFO88_09170"
     CDS             <1..213
                     /locus_tag="VFO88_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545737.1"
                     /translation="GPEKIFVAWFGPKGVASMLFALLVLASSDPNRTIVFDVASFVIL
                     ASIFAHGLTDTVGARWIERRLAPSDT"
     gene            248..1510
                     /locus_tag="VFO88_09175"
     CDS             248..1510
                     /locus_tag="VFO88_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET9545738.1"
                     /translation="MDVVVAVIFLVALAVIALEWVHRTKVALAGAGLVVILGILELEE
                     AIEAIDWATIGLLAGMMIIVGVTEKTGIFTYLALRAAQLSHGKPLRLVLLLGGVTGVM
                     SAFLDNLTAILLVVPITLLLADVLRITPIPLVLVEIFASNIGGTATLIGDPPNIMIGT
                     AVKELSFLDFIVNLAPVAFFTLGVVSVLLYFLFRNSLRVAPDRVAELQRLDPTRDVRE
                     AKNVKRSIAVLLGTIIAFFLHAPLHLEPAVVALGGATVLLLVAVDDVEEALERVEWAT
                     LFFFLGLFVMVGGLESQGIIEEVANLLGDLTDGNRTAEAMAVMWGGAAGSALVDNIPF
                     TAAMIPVVDQLQGADPVDDALWWSLALGACFGGNATMIAAAANVAATGVLDRTGHKIS
                     FVRFLLYGVPVTLLSLVIATVYIVVFQL"
     gene            1507..1968
                     /locus_tag="VFO88_09180"
     CDS             1507..1968
                     /locus_tag="VFO88_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9545739.1"
                     /translation="MKSILEDMALVDAAVGKDATYEDAARVLAASEAPTVAVLDDDRK
                     VVGLFGIEQALRGCMPSYLLEMRHTAFAEDDLAVLADRAREVLDDPILEHAVDPVTVD
                     LRTSAFHVAEVFLHEGMAGIAVVDQARYAGILGRAAFARAMLRRALADAGS"
     gene            2037..3584
                     /locus_tag="VFO88_09185"
     CDS             2037..3584
                     /locus_tag="VFO88_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9545740.1"
                     /translation="MAEQRKVGGVTYREAGEGYFEKRTLRRHAGVWSLWALGVAAVIS
                     GDFFGWNFGLAYGFGGLLIATIFVTVMYFGLCFSIAEMSPALPHTGGAYSFARSAMGP
                     WGGFTTGVAETIEYVITPAVIVVGIGSYMSSISDDLFGFTMADPIWWAIFYAVFVGLN
                     YVGVEASFRFTVFICLLSLAVLLVFFAGALTELDFQRFAIDAGGGWLPEGIPGIFYAL
                     PFAIWFYLAIEELPLAAEESADPERDIPRGTIWGLVTLVVTGFLVLFLNSGISPGSGE
                     LAESGEPLLEGLRTIFGDGTSASLLGLIAVAGLVASFHTIIFAYGRNIFSLSRAGYYP
                     HPFSVTHGTRQTPHVALIIGAIIGWLAALIIDQAGAGSTVGGSLLNMAVFGAVISYFM
                     QALSFVLLRRRLPNIKRPYRSPVGEWGALIAAAIALITLIAIFWNSDYRPGVYGVAIF
                     YLVALLYFALIGRHRLVFSPEEEFAMSHGERAGAHMEATATHLGGGLEAQMDEAPPPG
                     SGDEPRT"
     gene            3650..4984
                     /locus_tag="VFO88_09190"
     CDS             3650..4984
                     /locus_tag="VFO88_09190"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET9545741.1"
                     /translation="MLTLDELRNEVERGAIDTIVVAFTDMQGRLLGKRVHGEYFLSAG
                     VAEHGVEACDYLLALDMEMDPVPGYALANWELGYGDFVLRPDLGTLRRIPWLEATALL
                     LCDIVAHDGSDVAPSPRQVLKRQVERARELGYEPMFGSELEFYLFKESYAEAHEKGYQ
                     ELTPSVPYILDYHILASTFDEPFIRQVRNGMHAAGIPVEYSKGEAWPGQHEINFRYAD
                     AVTMADNHTIYKNGAKEIAYLNGLSLTFMAKPHEEWIGNSCHVHSSLWRDGEAAFAGE
                     SEVFRQYLAGQIAVLSELAVLVAPTVNSYKRFAAGSWAPTTLAWGHDNRTCGFRIVGH
                     GAGLRTETRIPGADANPYLCFAALLAAGLHGIENGLELPPPLEGNAYESDAARFPGTL
                     RDAIEALEHGSVARAALGDDVVDHYLTYARTEQRLFDGVVTEYERRRMFERG"
     gene            5122..5865
                     /locus_tag="VFO88_09195"
     CDS             5122..5865
                     /locus_tag="VFO88_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET9545742.1"
                     /translation="MTANDTPIVGITTYAENAAWGAWNVPTALVPLSYVRAVETAGGR
                     ALLVPPSEHAVEQTLDALDGLVFSGGADLDPAGYDADPHPETNGIRPERDRAELTLMG
                     AALERDMPILAVCRGMEIMNVLRGGDLLQHLPEVVGDEKHKHTPGVFADHDVSVADDS
                     RLARILGDRAPVKSHHHQGVGRVGDGLVETAWAEDGTIEGLEDPERSFALGVLWHPEE
                     DEDAALFRALVEEAAAYRARRTREGAAAP"
     gene            5862..7208
                     /locus_tag="VFO88_09200"
     CDS             5862..7208
                     /locus_tag="VFO88_09200"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9545743.1"
                     /translation="MNVVLNPATEEPIAELPHATAEDADRAVAAAKQAFPAWRAVAPE
                     DRARLLRRLAGLVEEHGEELAELESHNVGKPIADARGEVGMVADVFHFYAGAVDKHHG
                     ETIPVAGGVDLTFREPLGVVGLVTPWNFPLNIASWKLGPALACGNTVVLKPAELTPLT
                     SLRLGELALEAGIPEGVVNVVSGPGSVVGQRLVEHPDVAKIGFTGSTEVGRRVMESAA
                     GTIKRVTLELGGKSANVVFADADLERAAASAPGAVFGNAGQDCCARSRILVERSAYDR
                     FLELLEEATRAVKVGDPQSPDTEMGPLISAEHRENVASFVDGDARIRGDAPDGPGFWF
                     PCTVVEATNDDRVAREEVFGPVAAVIPFEDEADAIRIANDTPYGLSGSIWTENGARAL
                     RVARAIDSGVLSVNSNTSVRVQAPFGGFKQSGFGRELGLHALAGYSEVKNVYISTG"
     gene            7234..7743
                     /locus_tag="VFO88_09205"
     CDS             7234..7743
                     /locus_tag="VFO88_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545744.1"
                     /translation="MGRRLHVLLVALLAVALGAGCGATESAPEPDPEPDGIVLSSQQV
                     SDEFREETGRPLEKTAPDPSWEQLGFGLDPPPRVLREYGTFSIYVVDDESPKALESLL
                     TDKATGEPLEQDGEGIYWERDSLSNTWIAHSRYGENVVLAWFSESAEPETDERWERLD
                     EILAGLTTG"
BASE COUNT         1100 a         2798 c         2758 g         1216 t
ORIGIN      
        1 gggccggaga agatcttcgt cgcgtggttc ggcccgaagg gcgtcgcgtc gatgctcttc
       61 gccctcctcg tgctcgcctc gagcgacccg aaccggacga tcgtcttcga cgtcgcgtcc
      121 ttcgtgatcc tcgcgtcgat cttcgcgcac ggcctgacgg acaccgtcgg cgcgcgctgg
      181 atcgagcggc gactagcgcc gtccgacacg taagcagccg ttcggcgacc ccgtttagag
      241 tcgccgggtg gacgttgtcg tcgctgtcat cttcctcgtc gcgctcgccg tcatcgcgct
      301 cgagtgggtg caccggacga aggtggcgct cgccggcgcg gggctcgtgg tcatcctcgg
      361 gatcctcgag ctcgaggagg cgatcgaggc gatcgactgg gcgacgatcg gcctgctcgc
      421 cgggatgatg atcatcgtcg gcgtcaccga gaagaccggg atcttcacgt acctcgcgct
      481 ccgggcggcg cagctctcgc acggcaagcc gctgcgcctc gtgctcctcc tcggcggcgt
      541 caccggcgtc atgtcggcgt tcctcgacaa cctgacggcg atcctgctcg tcgtcccgat
      601 cacgctgctc ctcgccgacg tcctacggat cacgccgatc ccgctcgtcc tcgtggagat
      661 cttcgcgtcg aacatcggcg ggacggcgac gctgatcggc gacccgccga acatcatgat
      721 cggaactgcc gtcaaggagc tctcgttcct cgacttcatc gtgaacctcg cgccggtcgc
      781 gttcttcacg ctcggggtcg tctcggtgct tctctacttc ctcttccgga acagcctgcg
      841 cgtcgcgccg gaccgggtcg ccgagctgca gcggctcgat ccgacacggg acgtgcgcga
      901 ggcgaagaac gtcaagcgct cgatcgccgt gctcctcggg acgatcatcg cgttcttcct
      961 ccacgcgccc ctccatctcg agcccgcggt ggtcgcgctc ggcggggcga cggtcctcct
     1021 gctcgtcgcc gtcgacgacg tcgaggaggc gctcgagcgc gtcgagtggg cgacgctctt
     1081 cttcttcctc ggcctcttcg tcatggtcgg cgggctcgag agccagggga tcatcgagga
     1141 ggtcgcgaac ctgctcggcg acctcacgga cggcaaccgc acggccgagg cgatggcggt
     1201 catgtggggc ggcgcggccg gctcggcgct cgtcgacaat atcccgttca cggccgcgat
     1261 gatcccggtc gtcgatcagc tacagggcgc cgacccggtc gacgacgcgc tctggtggtc
     1321 gctcgccctc ggcgcgtgct tcggcgggaa cgcgaccatg atcgccgcgg ccgcgaacgt
     1381 cgccgcgacg ggcgtcctcg accggaccgg gcacaagatc tcgttcgtgc gcttccttct
     1441 gtacggcgta cccgtcacac tgctctcgct cgtgatcgcg accgtctaca tcgtcgtctt
     1501 ccagctgtga agagcatcct cgaggacatg gcgctcgtcg acgcggccgt cgggaaggac
     1561 gcgacgtacg aggacgcggc gcgcgtgctg gcggccagcg aggcgccgac ggtcgccgtg
     1621 ctcgacgacg accgcaaggt cgtggggctc ttcgggatcg agcaggcgct ccgcggctgc
     1681 atgccgagct acctactcga gatgcgccac accgccttcg cggaagacga cctcgcggtg
     1741 ctcgccgacc gggcgcgcga ggtcctcgac gacccgatcc tcgagcacgc cgtcgatccc
     1801 gtgacggtcg acctccgcac gagcgcgttc cacgtcgccg aggtcttcct ccacgagggg
     1861 atggccggga tcgcggtcgt cgaccaggcg cgctacgcgg ggatcctcgg ccgggcggcg
     1921 ttcgcccgcg cgatgctgcg ccgggcgctc gcggacgccg gctcttgacc gccgtccgca
     1981 cgccttctta ggatcgcgcc ttcctgcaca aaggaagggg gagtaggagg tgagctgtgg
     2041 cggagcagcg taaggtcggc ggcgtcacct accgcgaggc cggggagggt tacttcgaga
     2101 agaggaccct ccggcgccac gcaggagtgt ggtcgctctg ggcgctcggg gtggcggcgg
     2161 tcatctcggg cgacttcttc ggctggaact tcgggctcgc gtacgggttc ggcgggctcc
     2221 tgatcgcgac gatcttcgtc acggtcatgt acttcggcct gtgcttctcg atcgcggaga
     2281 tgtcgccggc tcttccccac accggcggcg cgtactcgtt cgcgcgctcg gcgatgggcc
     2341 cgtggggcgg cttcacgacg ggcgtcgcgg agacgatcga gtacgtcatc accccggcgg
     2401 tgatcgtcgt cgggatcggg agctacatga gctcgatctc ggacgacctg ttcgggttca
     2461 cgatggccga cccgatctgg tgggcgatct tctacgcggt cttcgtcggc ctgaactacg
     2521 tcggcgtcga ggcgagcttc cggttcaccg tcttcatctg cctcctctcg ctcgccgttc
     2581 tgctcgtgtt cttcgcgggc gcgctgacgg agctcgactt ccagcggttc gcgatcgacg
     2641 cgggcggcgg ctggctgccg gaggggatcc cggggatctt ctacgcgctg ccgttcgcga
     2701 tctggttcta cctcgcgatc gaggagctac cgctggcggc ggaggagtcg gccgacccgg
     2761 agcgcgacat cccgcgcggg acgatctggg gcctcgtgac gctcgtcgtg accggcttcc
     2821 tcgtcctctt cctgaacagc ggcatctcgc ccggctcggg tgagctcgcg gaatccggag
     2881 agccgctcct cgagggcctg cggacgatct tcggcgacgg cacctccgcg tcgctcctcg
     2941 gcctgatcgc ggttgccggc ctcgtcgcca gcttccacac gatcatcttc gcgtacgggc
     3001 ggaacatctt ctcgctctcg cgcgccggct actacccgca cccgttctcg gtcacccacg
     3061 gcacgcggca gacgccgcac gtcgcgctca tcatcggcgc gatcatcggg tggctcgcgg
     3121 cgctcatcat cgaccaggcc ggcgccggct cgacggtcgg cggctcgctg ctcaacatgg
     3181 ccgtgttcgg cgcggtcatc tcgtacttca tgcaggcgct ctcgttcgtc ctcctgcggc
     3241 ggaggctgcc gaacatcaag cggccctacc ggagcccggt cggtgagtgg ggagccctca
     3301 tcgccgcggc gatcgcgctc atcacgctga tcgcgatctt ctggaactcc gactaccggc
     3361 ccggcgtgta cggcgtggcg atcttctacc tggtcgccct gctctacttc gcgctgatcg
     3421 gccggcaccg gctcgtgttc tcgccggagg aggagttcgc gatgtcgcac ggcgagcggg
     3481 ccggcgcgca catggaggcg acggcgacgc atctcggcgg cggcctcgag gcgcagatgg
     3541 acgaggcgcc tccgcccggt tcgggagacg agccgaggac gtagactcgc cgcacgattc
     3601 ggcggggagg gccggcggcg cgccggccct ccccgctccg gaggcccgaa tgctgacgct
     3661 ggacgagctg cggaacgagg tggaacgcgg cgcgatcgac acgatcgtcg tcgcgttcac
     3721 ggacatgcaa gggcgcctgc tcggcaagcg cgtccacggc gagtacttcc tctccgccgg
     3781 cgtggccgag cacggggtcg aggcgtgcga ctacctgctc gcgctggaca tggagatgga
     3841 cccggtcccg ggctacgcgc tcgcgaactg ggagctcggg tacggcgact tcgtcctgcg
     3901 gcccgacctc gggacgctcc gccggatccc ctggctcgag gcgacggcgc ttctcctctg
     3961 cgacatcgtc gcgcacgacg gctcggacgt cgcgccgtcg ccgcgccagg tgctcaagcg
     4021 ccaggtcgag cgggcgcgcg agctcggcta cgagccgatg ttcgggagcg agctcgagtt
     4081 ctacctcttc aaggagtcgt atgccgaggc gcacgagaag ggctaccagg agctgacgcc
     4141 gtccgtgccc tacatcctcg actaccacat cctcgcgtcg accttcgacg agccgttcat
     4201 ccggcaggtt cggaacggga tgcacgccgc gggaatcccg gtcgagtact cgaagggcga
     4261 agcgtggccg ggtcagcacg agatcaactt ccgctacgca gacgcggtca cgatggccga
     4321 caaccacacg atctacaaga acggcgcgaa ggagatcgcg tacctgaacg ggctctcgct
     4381 cacgttcatg gcgaagcccc acgaggagtg gatcgggaac tcgtgccacg tccactcgtc
     4441 actctggcgc gacggcgaag cggcgttcgc gggcgagtct gaggtcttcc ggcagtacct
     4501 cgcaggccag atcgcggtgc tctcggagct cgcggtgctc gtcgcgccga cggtcaactc
     4561 gtacaagcgg ttcgcggccg gctcgtgggc gccgacgacg ctcgcttggg gccacgacaa
     4621 ccggacgtgc gggttccgga tcgtcgggca cggcgccggg ttgcggacgg agacccgaat
     4681 ccccggtgcc gacgcgaacc cgtacctctg cttcgccgcg ctcctcgcgg ccgggctaca
     4741 cgggatcgag aacgggctcg agctaccgcc gccgctcgag gggaacgcgt acgagtcgga
     4801 cgcggcgcgc ttccccggca cgctgcgtga cgcgatcgag gcactggagc acggctcggt
     4861 cgcccgcgcc gcgctcggcg acgacgtcgt cgaccactac ctcacgtacg cgcgcacgga
     4921 gcagcggctc ttcgacgggg tcgtgacgga gtacgagcgc cggcggatgt tcgagcgcgg
     4981 ctagcgcgga atcggcacgg atccgtcacg aagccggcac attccgtcac agacacgcgc
     5041 gtgtttccgc attagggttt gaaccaaggg gtgggggcga acctcccgtt acacgcgcgt
     5101 gcggaaagta aagtccgggc gatgaccgcg aacgacacgc cgatcgtcgg gatcaccacc
     5161 tacgcggaga acgcggcctg gggcgcgtgg aacgtgccga ccgcgctcgt gccgctctcg
     5221 tacgtccgcg cggtggagac cgccggcggg cgcgcgctgc tcgtcccgcc ctccgagcac
     5281 gcggtcgagc agacgctcga cgccctcgac gggctcgtct tctcgggcgg cgccgacctc
     5341 gacccggccg gctacgacgc cgacccgcac cccgagacga acgggatccg ccccgagcgc
     5401 gaccgcgccg agctcacgct catgggcgcg gcgctcgagc gggacatgcc gatcctcgcc
     5461 gtctgccgcg ggatggagat catgaacgtc ctgcgcggcg gcgacctcct ccagcacctg
     5521 cccgaggtcg tcggggacga gaagcacaag cacacgccgg gcgtcttcgc cgaccacgac
     5581 gtgtcggtcg cggacgacag ccgcctcgcg cgcatcctcg gcgaccgcgc cccggtcaag
     5641 tcgcaccatc accagggcgt cggccgcgtc ggcgacgggc tcgtcgagac cgcgtgggcg
     5701 gaggacggca cgatcgaggg cctcgaggat ccggagcgga gcttcgcgct cggcgtcctc
     5761 tggcatccgg aggaggacga ggacgcggcg ctcttccgcg cgctcgtcga ggaggccgcc
     5821 gcctaccggg cgcggcgcac ccgagaggga gcggcggcgc cgtgaacgtc gtcctgaatc
     5881 ccgcgaccga ggagccgatc gccgagctcc cgcacgcgac ggccgaggac gcagaccgcg
     5941 ccgtcgccgc cgccaagcaa gcgttccccg cctggcgcgc cgtcgcgccc gaggatcgag
     6001 cccgcctgct ccggcgcctc gcaggcctcg tcgaggagca cggcgaggag ctcgccgagc
     6061 tcgagtccca caacgtcggc aagccgatcg ccgacgcgcg cggcgaggtc gggatggtcg
     6121 cggacgtctt ccacttctac gccggcgccg tcgacaagca ccacggcgag accattccgg
     6181 tcgcgggcgg cgtcgacctc acgttccgcg agccgctcgg cgtcgtcggc ctcgtcacgc
     6241 cgtggaactt cccgctcaac atcgcctcgt ggaagctcgg tccggcgctc gcctgcggca
     6301 acacggtcgt cctcaagccg gccgagctga ccccgctcac gtcgctccgc ctcggcgagc
     6361 tcgcgctcga ggccgggatc cccgaaggcg tcgtgaacgt cgtctcgggc cccggctcgg
     6421 tcgtcgggca gcggctcgtc gagcacccgg acgtggcgaa gatcggcttc accggctcga
     6481 ccgaggtcgg ccgccgcgtg atggagagcg ccgccgggac gatcaagcgc gtcacgctcg
     6541 agctcggcgg caagtcggca aacgtcgtct tcgcggacgc cgacctggag cgcgccgccg
     6601 cctccgctcc gggcgcggtc ttcgggaacg ccggccagga ctgctgcgcg cgctcgcgga
     6661 tcctcgtcga gcggagcgcg tacgaccgct tcctcgagct gctcgaagaa gccacgcgcg
     6721 cggtgaaggt cggcgacccg cagagccccg acaccgagat gggcccgctc atctcggccg
     6781 agcaccggga gaacgtcgcc tccttcgtgg acggcgacgc gcgcatccgc ggcgacgcgc
     6841 ccgacggccc cggcttctgg ttcccgtgca ccgtcgtcga ggcgacgaac gacgaccgcg
     6901 tcgcgcgcga ggaggtcttc ggccccgtgg cggccgtgat cccgttcgag gacgaggccg
     6961 acgcgatccg gatcgcgaac gacacgccgt acgggctctc gggctcgatc tggacggaga
     7021 acggcgcccg cgcgctccgc gtcgcgcgcg cgatcgactc gggtgtgctc tccgtgaact
     7081 cgaacacgag cgtgcgcgtc caggcgccgt tcggcgggtt caagcagtcc gggttcggcc
     7141 gcgagctcgg cctccacgcg ctcgccggct actcggaggt aaagaacgtg tacatctcca
     7201 ccggctagcc ggacgttcgc ctaccctgac ggcgtgggcc gccgcctcca cgtgctcctc
     7261 gtcgcgctcc tcgcggtcgc gctcggcgcg gggtgcgggg cgaccgagtc ggcgcccgag
     7321 cccgaccccg agccggacgg gatcgtcctc agctcgcagc aggtctcgga cgagttccgg
     7381 gaggagaccg ggcgcccgct cgagaagacc gcgccggacc cgtcgtggga gcagctcggc
     7441 ttcggcctcg acccgccgcc gcgcgtcctc cgcgagtacg ggacgttctc gatctacgtc
     7501 gtggacgacg agagccccaa ggcgctcgag tcgctcctca cggacaaggc gaccggcgag
     7561 ccgctcgagc aggacggcga ggggatctac tgggagcgcg actcgctcag caacacctgg
     7621 atcgcgcact cccgctacgg cgagaacgtc gtgctcgcct ggttcagcga gagcgcggag
     7681 ccggagaccg acgagcgttg ggagcggctc gacgagatcc tcgccggcct cacgaccggg
     7741 tagattccgc cgaccggcaa ggcggaaagg aggcggctcg tgggcaggct ggacgggaag
     7801 gtcgtggtga tcacgggggc ggccggaggg atcgggcgcg aggccgcgct cctcttctcg
     7861 ggcgaaggcg cc
//
LOCUS       DASTFC010000311         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1013388,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000311 DASTFC010000000
VERSION     DASTFC010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013388"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFO88_09210"
     CDS             complement(<1..461)
                     /locus_tag="VFO88_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545745.1"
                     /translation="MSLDLGVLVASRGGELRVHVNRPVYWLPPAGKQVDAETLAVLRT
                     LPPEALDGFRGLAGFVVVTARKPGGRFVAQRSFTFCPNTYARQRVDDSGPVLPHYPFG
                     CGSSSPFAQGMVWGIEAGWATGLFSEGEESSWFDSTMPLRPGSYVLTYAIAP"
     gene            complement(683..1105)
                     /gene="msrB"
                     /locus_tag="VFO88_09215"
     CDS             complement(683..1105)
                     /gene="msrB"
                     /locus_tag="VFO88_09215"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9545746.1"
                     /translation="MTDREEASMPNEVRKSEEEWRRELTPEQYHVLREKGTERPWTGK
                     YATTKEHGTFRCAGCGAELFRSDTKYESGSGWPSFYAPVADDAVETEEDRSLGMTRTE
                     ALCASCGGHLGHVFPDGPHPTGLRYCINSAALDLAPDE"
     gene            1157..>2287
                     /locus_tag="VFO88_09220"
     CDS             1157..>2287
                     /locus_tag="VFO88_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9545747.1"
                     /translation="MSNLLLVPLEDAVVFPHMDVTLTVDVGDEERVLLVPRHENEFAS
                     VGTVAEVGEQVRLPAGVRAVALSGLHRGIAGAASTDARGRLRVEVEERPDDDQPVDGR
                     IKELESEYRAVVEEILELRGDDGRIAAFVRSITEPGALADTVGYAPDFTFEQKVSVLE
                     AVDLRERLELAVKLQRERLAELQVRRRIRDDVESGAQKQQREYFLRKQMESIRKELGQ
                     DEGSVIDEYRAKIEEAGMPEEVREQAERELARLESIGEQSAESSVIRTYLDWLIVVPW
                     SKRSEERLDPAYAREVLDNDHYGLEDVKDRIVEYLAVRKLRKERGIEEDKRSGAILTL
                     IGPPGTGKTSIGESVARALGREFVRMSLGGVRDEAEIRGHRRT"
BASE COUNT          336 a          817 c          826 g          308 t
ORIGIN      
        1 ggcgcgatcg cgtaggtcag gacgtagctc cccggccgga gcggcatggt cgagtcgaac
       61 cacgacgact cctcgccctc cgagaagagc ccggtcgccc agccggcctc gatgccccag
      121 accatcccct gtgcgaacgg cgacgaggat ccgcagccga acgggtagtg cgggaggacc
      181 ggccccgagt cgtcgacgcg ctgacgtgcg taggtgttcg ggcagaacgt gaagctccgc
      241 tgcgcgacga acctgccgcc gggcttgcgc gcggtgacga cgacgaagcc cgcgagaccg
      301 cggaagccgt cgagcgcctc gggcgggagc gtccggagca ccgcgagcgt ctccgcgtcg
      361 acctgctttc cggccggcgg gagccagtac accgggcggt tgacgtgcac ccggagctct
      421 ccgccgcgcg acgcgacgag cactccgagg tcgaggctca cccggccgcg gaacgggctc
      481 gccgtccgcg ggcgctccgg gccgagcagc tcgagcgacg ggacctgcgc gccggccgcg
      541 ggcgcgaggc cgagcgccgc gagcgcgatg cacgcggcgg ccgcgagccg cagccgccgt
      601 ccgctccgat cccgtgcccc gcgcaggcac cggatcgtag tcctcgcccc ggcggtttgg
      661 aagacccggg gcgtcgaggt ctctactcgt ccggcgcgag gtcgagcgcg gccgagttga
      721 tgcagtagcg caggcccgtc gggtgcggcc cgtccgggaa gacgtgcccg aggtggccgc
      781 cgcaggacgc gcagagcgcc tccgtacggg tcatcccgag cgagcggtcc tcctccgtct
      841 cgaccgcgtc gtccgcgacg ggcgcgtaga agctcggcca gccgcttccc gactcgtact
      901 tcgtgtcgga gcggaagagc tccgcgccgc agcccgcgca gcggaacgtg ccgtgctcct
      961 tcgtcgtcgc gtacttgccc gtccagggcc gctccgtgcc cttctcacgg aggacgtggt
     1021 actgctcggg cgtcagctcg cggcgccact cctcctcgct ctttcgcacc tcgttcggca
     1081 tcgaggcctc ctcccggtcg gtcactacag aatgtatact tgctcgcgtc ttagtactag
     1141 gtaaggagtc tgtttcgtga gcaacctctt gctcgtccct ctggaagacg cagtcgtctt
     1201 cccccacatg gacgttacgc tcaccgtgga cgtcggtgac gaggagcgcg tgctgctcgt
     1261 cccccgccac gagaacgagt tcgcgagcgt cggcacggtc gccgaggtcg gcgagcaggt
     1321 ccgcctgccc gccggcgtgc gcgccgtcgc gctctccggc ctccaccggg gcatcgccgg
     1381 ggcggcctcg acggacgcgc ggggccgcct gcgcgtcgag gtcgaggagc ggcccgacga
     1441 cgaccagccg gtcgacggcc gcatcaagga gctcgagagc gagtaccgcg cggtcgtcga
     1501 ggagatcctc gagctgcgcg gtgacgacgg gcgcatcgca gccttcgtcc gctcgatcac
     1561 cgagccgggc gccctcgccg acacggtcgg ctacgcaccc gacttcacgt tcgagcagaa
     1621 ggtctccgtc ctcgaggcgg tcgacctgcg cgagcggctc gagctcgccg tgaagctcca
     1681 gcgggagcgc ctcgccgagc tgcaggtccg ccgccggatc cgcgacgacg tcgagtcggg
     1741 cgcccagaag cagcagcgcg agtacttcct gcgcaagcag atggagtcga tccgcaagga
     1801 gctcggccag gacgagggct ccgtgatcga cgagtaccgc gcgaagatcg aggaggccgg
     1861 gatgcccgag gaggtgcggg agcaggccga gcgcgagctc gcgcgcctcg agtcgatcgg
     1921 cgagcagagc gcagagagct cggtcatccg cacgtacctc gactggctca tcgtcgtgcc
     1981 gtggtcgaag cgctccgagg agcggctcga ccccgcgtac gcgcgcgagg tcctcgacaa
     2041 cgaccactac ggtctcgagg acgtgaagga ccggatcgtc gagtacctcg cggtccgcaa
     2101 gctccgcaag gagcgcggga tcgaggagga caagcgctcc ggcgcaatcc tcacgctgat
     2161 cggcccgccc gggaccggga agacgtcgat cggcgagtcg gtcgcgcggg cgctcgggcg
     2221 cgagttcgtc cggatgtcgc tcggcggcgt ccgggacgag gccgagatcc gcggccaccg
     2281 gcgcacg
//
LOCUS       DASTFC010000312         4520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_486307,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000312 DASTFC010000000
VERSION     DASTFC010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486307"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VFO88_09225"
     CDS             complement(<1..1319)
                     /locus_tag="VFO88_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545748.1"
                     /translation="MAALVDTTIRLLSQEPLAGRVQTARLLEVADGLDRAGFAALEVT
                     GGGCFDAAVRRGVESPWERIRTLRARCETPLGMALRGRFLVGSRPLSSDVVRRFVKTA
                     AGNGIDVFRIHDPLNDLTNLREAAEAIHAAEKELSVGLVHSPGPADATDALLERAQHL
                     ADLSPARVLIHDPTGSLDPATARELVERVRELSGLPVGLYVQGAGGSALASALEAARA
                     GADLIACAIYPVALSLYRVSAESAARSLAGLGQDPGVDLDLLWEVCELVDDAIGEEPV
                     APLSPRVAVRAAEHSLPAGLVAELDAHLRSQSFGDRLDAVLEELTAIRREAGWPPLAS
                     PIGQVLGSQALIHVLSGARWQIVVDELRDLVEGRYGSPPGEIDPAVRRAISLVREAGP
                     ADGEPPVELDALREHAEGIAASEEELLLLALFGDQAEGLLRAIRARGR"
     gene            complement(1329..1895)
                     /gene="efp"
                     /locus_tag="VFO88_09230"
     CDS             complement(1329..1895)
                     /gene="efp"
                     /locus_tag="VFO88_09230"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET9545749.1"
                     /translation="MAEQVSTNQFKNGMHVQVDGQIWRIVEFQHVKPGKGGAFVRTKL
                     KSVETGSVVDKTFRAGEKMPRIRTETKSVQYLYDAGDEVVFMDEGSFEQINLPRADVG
                     GELPYLQPSSSVQLLLVDGKPAGIQLPASVELQVTETEPGVRGDTVSNVTKPATLETG
                     AVVQVPLFVDVGERIKVDPREGRYISRA"
     gene            complement(1974..3035)
                     /locus_tag="VFO88_09235"
     CDS             complement(1974..3035)
                     /locus_tag="VFO88_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9545750.1"
                     /translation="MSAARRDRLRAELARSGPDTLLVTAPANVRYLTGFESSNAAVLV
                     GRDAATLLTDGRYVEAARAIEGVELVQSSRDLASFLGERLGDLVSGPVAFESGHVTHA
                     AWQALAGSGVELVPTAGVVERLRAVKEPGELDAIRSAAAVTNAVYERLAEEGGVLGAT
                     EAELAWRLAGFVHDAAADGLAFETIAATGPNAALPHHHPGGRRIEPGQTLIVDLGAAL
                     DGYASDCTRTFATGPLPDELARAYEACREAQAESLAAVRAGASARDVDAVARRRLEAE
                     GYEILHGLGHGVGLEVHEAPRLADTSDAVLEAGNVVTVEPGVYLAGVGGVRIEDLVIV
                     TEGEPEVLTAFTKELVTLA"
     gene            complement(3032..3460)
                     /locus_tag="VFO88_09240"
     CDS             complement(3032..3460)
                     /locus_tag="VFO88_09240"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET9545751.1"
                     /translation="MQIAVLNGVNLNALGDRDPALYGGLSLNELENRIYRWGRELGLT
                     VRCQQTNHEGSFVDWCHEARTWADGVIVNPGAWSHYSYAIRDAVELFECPVVEVHLSD
                     IAKREEWRRQSVLEDVVATRVIGKGPEGYREALVHLAERR"
     gene            3590..4063
                     /locus_tag="VFO88_09245"
     CDS             3590..4063
                     /locus_tag="VFO88_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545752.1"
                     /translation="MERFIKTTLAGLAAVVAVTGFVFATAPMATPSGDAAMDEVAIKR
                     DDDDVELELARLDEDDDGDDDELATGAEFAAAGSGDISRSRDRSRDVTSADRSRSRDK
                     SRDRTGDRVGVADRSKSLDRSRDRTGDHDSRDISRSRDASGDDTSVSLSNSNSFA"
     gene            4060..>4520
                     /locus_tag="VFO88_09250"
     CDS             4060..>4520
                     /locus_tag="VFO88_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9545753.1"
                     /translation="MSFEALNEGPEARGRRPEGDAAPERDGWALEEGDAIAPGRTAVR
                     LLGGGERYESYLAWDDRLLSLVVVKIVRPSLVHDAHSLEGLASEADLLLRLRHPVLVR
                     GFDAVLDGPRPHVVLEHLEGSRLSSLIRKQGPLEPEQLYPLGLQLASAVHYL"
BASE COUNT          599 a         1685 c         1646 g          590 t
ORIGIN      
        1 cggccgcggg cgcggatcgc gcgcaggagg ccttccgcct ggtcgccgaa gagcgcgagc
       61 aggagcagct cctcctcgct cgcggcgatg ccctcggcgt gctcgcggag cgcgtcgagc
      121 tccacgggcg gctccccgtc cgcgggcccg gcctcgcgga cgaggctgat ggcgcgccgc
      181 acggccgggt cgatctcgcc cggcggcgac ccgtagcggc cctcgacgag gtcgcggagc
      241 tcgtccacga cgatctgcca gcgcgccccc gagagcacgt ggatgagcgc ctgcgagccg
      301 agcacctgcc cgatcggcga cgcgagcggg ggccagcctg cctcccggcg gatcgcggtc
      361 agctcctcga ggacggcgtc gagccggtcg ccgaacgact ggctgcggag atgggcgtcg
      421 agctcggcga cgagcccggc cggaaggctg tgctcggccg cccgcacggc gacgcgtggc
      481 gagagcggcg ccaccggctc ctcgccgatc gcgtcgtcca cgagctcgca cacctcccag
      541 agcaggtcga ggtcgacccc cgggtcctgg ccgagcccgg cgagcgagcg cgcggccgac
      601 tccgccgaca cgcggtacag cgagagcgcg accggataga tcgcgcacgc gatcaggtcg
      661 gcgccggcgc gcgcggcctc gagcgccgag gcgagcgccg agccccctgc gccctggacg
      721 tacagcccga ccggcaaccc gctgagctcc cggacccgct cgacgagctc gcgggccgtg
      781 gcggggtcga gcgacccggt cggatcgtgg atgaggacgc gtgcgggcga gaggtcggcg
      841 aggtgctgcg cccgctcgag caaggcgtcg gtcgcgtcgg ccggcccggg gctgtggacg
      901 agccccacgg agagctcctt ctctgccgcg tggatcgcct cggccgcctc gcggaggttc
      961 gtgaggtcgt tcagcggatc gtggatgcgg aagacgtcga tcccgttccc cgccgccgtc
     1021 ttcacgaagc ggcggacgac gtcgctcgag agcggtcgcg acccgacgag gaagcggccg
     1081 cggagcgcca tcccgagcgg cgtctcgcag cgcgcgcgca gcgtccggat ccgctcccac
     1141 gggctctcaa ccccgcgccg cacggccgcg tcgaagcagc cgccgccggt cacctcgagc
     1201 gccgcgaacc ccgcccgatc gagcccgtcg gcgacctcca ggagccgcgc cgtctggacg
     1261 cgccccgcga gcggctcctg ggagaggagc cggatcgtcg tgtcgacgag cgcggccatg
     1321 ggcgcagtct aagcccggga gatgtagcgg ccctcgcgcg gatcgacctt gatccgctcg
     1381 ccgacgtcga cgaagagcgg gacctggacg accgcccccg tctcgagggt cgccggcttc
     1441 gtcacgttcg agaccgtgtc gccgcggacg ccgggctcgg tctccgtcac ctggagctcg
     1501 acggacgccg gaagctggat cccggcgggc ttcccgtcca cgagcaggag ctgcaccgag
     1561 gacgacggct gcaggtacgg cagctcgccg ccgacgtcgg cgcggggcag gttgatctgc
     1621 tcgaacgagc cctcgtccat gaagacgacc tcgtcgcccg cgtcgtagag gtactggacg
     1681 ctcttcgtct ccgtgcggat ccgcggcatc ttctcgcccg cgcggaacgt cttgtcgacg
     1741 acggagcctg tctcgacgct cttcagcttc gtgcgcacga acgcgccgcc cttgccgggc
     1801 ttcacgtgct ggaactcgac gatgcgccag atctgcccgt ccacctggac gtgcatcccg
     1861 ttcttgaact ggttcgtgga gacctgctcg gccacgggcg acgattgtag tgacgaccgc
     1921 gctacgaggc gccggccgtt ccccggtcgc cggcggcttg gtcggcccga ccgctacgcg
     1981 agcgtcacga gctccttcgt gaacgccgtc agaacctcgg gctccccctc ggtcacgatc
     2041 acgaggtcct cgatgcggac cccgccgacg cccgcgaggt agacgcccgg ctcgacggtc
     2101 acgacgttcc ccgcctcgag caccgcgtcc gaggtgtccg cgagccgggg cgcctcgtgc
     2161 acctcgagcc cgaccccgtg gccgagcccg tggaggatct cgtagccctc cgcctcgagc
     2221 cggcgccggg caaccgcgtc gacgtcgcgt gcggacgcgc cggcgcgcac cgcggcgagc
     2281 gactcggcct gggcctcgcg gcaggcctcg tacgcgcggg cgagctcgtc ggggagcggg
     2341 cccgtggcga aggtccgggt gcagtcggac gcgtagccgt cgagggccgc gccgaggtcg
     2401 acgatcagcg tctggccggg ctcgatgcgc cggccgccgg gatggtgatg cgggagcgcc
     2461 gcgttcggcc cggtcgcggc gatcgtctcg aaggcgagcc cgtcggcggc cgcgtcgtgg
     2521 acgaaccccg cgagccgcca cgcgagctcg gcctcggtcg cgccgaggac gcctccctct
     2581 tcggcgagcc gctcgtacac cgcgttcgtg acggctgcgg cgctgcgaat cgcgtcgagc
     2641 tcgccgggct ctttcaccgc gcggaggcgc tcgacgacac ccgccgtcgg gacgagctcg
     2701 acgccgcttc cggcgagcgc ctgccaggcg gcgtgggtca cgtggccgga ctcgaacgcc
     2761 acggggccgg aaacgaggtc gccgagccgc tcgccgagga aggacgcgag atcgcgcgag
     2821 gactggacga gctcgacgcc ctcgatcgcc cgcgccgcct cgacgtagcg gccgtcggtc
     2881 aggagcgtcg ccgcgtcccg gccgacgagg acggccgcgt tcgagctctc gaagcccgtc
     2941 aggtagcgga cgttcgccgg ggcggtgacg aggagcgtgt ccgggccgct tcgcgccagc
     3001 tcggcccgga ggcgatcccg ccgggcggcg ctcaccggcg ctccgccagg tggacgagcg
     3061 cctcgcggta gccctccggg cccttcccga tcacgcgcgt agcgacgacg tcctcgagga
     3121 cggactggcg ccgccattcc tcgcgcttcg cgatgtcgga gaggtgcacc tcgacgaccg
     3181 ggcactcgaa gagctcgacc gcgtcgcgga tcgcgtagct gtagtgcgac caggcgcccg
     3241 ggttcacgat gacgccgtcc gcccaggttc gcgcctcgtg gcaccagtcg acgaagctcc
     3301 cctcgtggtt cgtctgctgg cagcggacgg tgagcccgag ctcgcgcccc cagcgataga
     3361 tccggttctc gagctcgttg aggctgaggc cgccgtagag ggcagggtcg cggtccccga
     3421 gcgcgttcag gttcaccccg ttcaggacgg cgatctgcac gcggcgagtc tagtgccgcg
     3481 catggtgcgg ccacgatgag ggaaatctca tggacgtttc atgctcggct catggcgcgg
     3541 ccgtacggtg gcctcatcga ccaccaccga ccgaaggggg tacgccgcaa tggaacgctt
     3601 catcaagacg acgctcgcag gcctcgccgc cgtggtcgcc gtgaccgggt tcgtcttcgc
     3661 gaccgcgccc atggcgacgc cgtccggcga cgccgcgatg gacgaggtcg cgatcaagcg
     3721 ggacgacgac gacgtcgagc tcgagctcgc acgcctcgac gaggacgacg acggcgacga
     3781 cgacgagctc gcgaccggcg ccgagttcgc agccgcgggc agcggggaca tctcccgctc
     3841 gcgcgaccgc tcccgcgacg tcacgtcggc cgaccgctcg cgctcccgcg acaagtcgcg
     3901 cgatcgcacc ggcgaccgcg tgggcgtggc ggaccgctcg aagtcgctcg accgctcgcg
     3961 cgaccgcacg ggcgaccacg actcgcgcga catctcccgc tcgcgggacg cctcgggcga
     4021 cgacacgagc gtgagcctct ccaactcgaa ctccttcgcg tgagcttcga ggctctgaac
     4081 gaggggcccg aagcccgggg gcggcgtccc gagggggatg ccgcccccga gcgcgacggc
     4141 tgggcgctcg aggagggcga cgcgatcgcg cccggccgca cggccgtccg gctgctcggc
     4201 ggcggggagc ggtacgagtc gtacctcgcg tgggacgacc ggctcctctc gctcgtcgtc
     4261 gtgaagatcg tcaggccgag cctcgtccac gacgcgcatt cgctcgaggg gctcgcgagc
     4321 gaggccgacc tcctgctccg cctccgccac ccggtgctcg tgcgcgggtt cgacgcggtg
     4381 ctcgacggcc cgaggccgca cgtcgtgctc gagcacctcg aggggtcgcg gctctcgtcg
     4441 ctcatccgga agcaggggcc gctcgagccc gagcagctgt acccgctcgg cctgcagctc
     4501 gcctccgccg tccactacct
//
LOCUS       DASTFC010000313         6238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_74022,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000313 DASTFC010000000
VERSION     DASTFC010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74022"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..654)
                     /gene="rplS"
                     /locus_tag="VFO88_09255"
     CDS             complement(310..654)
                     /gene="rplS"
                     /locus_tag="VFO88_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9545754.1"
                     /translation="MASPIDLIEQRQLRDGLPQFKAGDTVRVHFQVIEGQRRRVQVFE
                     GIVIKRQGAGARETFTVRKSSFGVGVERTFPLHSPKIEKIEVQAIGDVNRAKLYYLRG
                     KVGKKARVREKR"
     gene            complement(694..1302)
                     /gene="lepB"
                     /locus_tag="VFO88_09260"
     CDS             complement(694..1302)
                     /gene="lepB"
                     /locus_tag="VFO88_09260"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9545755.1"
                     /translation="MRGPVDRLTGWIPEPWRAVVDWVVTIAVAIIVVLAIKAYVVNPY
                     RIPSSSMEPTLHCAEPGVGCQSSFSDRVLANRFIYHFKDPERGDVIVFKTPPEASRCD
                     FGLAGETFVKRLVGLPGERIREQNGRILINGEPLDEPYLDPSQRDGSSFPEQTIPEGH
                     YFMLGDNRNQSCDSRVWGPVPRGNIIGEVFAVYWPPQRIGFR"
     gene            complement(1299..1979)
                     /gene="trmD"
                     /locus_tag="VFO88_09265"
     CDS             complement(1299..1979)
                     /gene="trmD"
                     /locus_tag="VFO88_09265"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET9545756.1"
                     /translation="MQIDVFTLIPHAFSWMTEHRPLAAVLGSELDLRLWSYRDFTPLR
                     AGQVDDEPYGGGAGMVLRVDVVHAALDAVYAGDWSDRRVVALTPQGRPLDQELVEELA
                     AEERLTLLSARSEGFDERVARHLATDAVSIGPYVLSGGELPAMVVLDAVARRLPGALR
                     AESGAVESFSAALEGGLEYPHFTRPPDFRGWRVPDVLLSGDHGRIEEWRREQSRTRSS
                     GGGLVGAR"
     gene            complement(2039..2506)
                     /gene="rimM"
                     /locus_tag="VFO88_09270"
     CDS             complement(2039..2506)
                     /gene="rimM"
                     /locus_tag="VFO88_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9545757.1"
                     /translation="MADRGWVAVGFVGRPHGLRGDFVVEHASEAPERFARGARVYVGR
                     EPATVVESKRAGRGRLVVRLDRAVERGAALELPVDELPEPEEGSYYAFQLVGLAVEED
                     GGEVLGSVQEVEPGVANDVLRLDSGVALPLVEDCVREVDLAGGRILVARGFAV"
     gene            complement(2499..2744)
                     /locus_tag="VFO88_09275"
     CDS             complement(2499..2744)
                     /locus_tag="VFO88_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9545758.1"
                     /translation="MAATPVEEMLVHLARSLVSEPDAVRVERTEREGAIVLELHVAEA
                     DLGKVIGKGGRIARALRTLVRASGAKADERRLLEIVG"
     gene            complement(2759..3010)
                     /gene="rpsP"
                     /locus_tag="VFO88_09280"
     CDS             complement(2759..3010)
                     /gene="rpsP"
                     /locus_tag="VFO88_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9545759.1"
                     /translation="MATRIRLARVGSKKNPIYRVVVADGRSPRDGRFIEIVGRYNPQT
                     DPSTIDLDTDKIQAWIEKGAQPSESVRRLLKAQASGPPS"
     gene            complement(3023..4357)
                     /gene="ffh"
                     /locus_tag="VFO88_09285"
     CDS             complement(3023..4357)
                     /gene="ffh"
                     /locus_tag="VFO88_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9545760.1"
                     /translation="MFESLSDKLQLTLGDLRGRGRLSEEDLSRAAREIRLALLEADVN
                     FQVVKGFVATVKERSLGADVTKSLTPGQEVIKIVHEELTALMGSGGSKLAFAGRPPTV
                     ILLAGLQGSGKTTASAKLALHLRKQGKTPALVAADLQRPAAIDQLEQLGKQIQIPVYL
                     DRQTKDAVAVAKHGLEQARAQGRDVLVVDTAGRLHIDEELMDELAAVRAAVKPLNVLL
                     VLDAMTGQDAVTTAEAFAERIDFDGVVLTKLDGDARGGAALSVKAVTGKPIKLVSTGE
                     KLDQLEEFHPDRMASRILGMGDVLTLIEKAEAAVEEDDQEAMERRLRAGQFTFDDFLQ
                     AQRMMRKMGPLQSVMGMIPGLGKQMKGVNVDERHLARVEAIVLSMTADERARPEIING
                     SRRARIARGSGTSVQQVNQLLQARKQMQKMMKQLGQGKMPNLQSIVAQQVRR"
     gene            4455..4664
                     /locus_tag="VFO88_09290"
     CDS             4455..4664
                     /locus_tag="VFO88_09290"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET9545761.1"
                     /translation="MADVAKVVTVIGNSPESFAQAAQVAVEEASKTLRGITGADVISM
                     SCEVADGRITTYRTTVNIAFALERA"
     gene            complement(4674..5360)
                     /locus_tag="VFO88_09295"
     CDS             complement(4674..5360)
                     /locus_tag="VFO88_09295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9545762.1"
                     /translation="MADRVLVITGASSGIGEATARLAAADGFRLVLAARRLERLEGLA
                     AELGGEERARAVRCDVTEWDDVRALADAALDAFGSIDVVFANAGFGATRGFEASTPEH
                     WRAMVLTNIYGAALTLRATFPALKASRGHLLLTGSVAGRRVLSGSLYSATKWAVTAMG
                     EAARQELDGTGVRVTLIEPGTVATPFFDDPVTDALEPEDVARAVLYAVGQPPHVDVNE
                     VLVRPTAQSS"
     gene            complement(5507..>6238)
                     /gene="ftsY"
                     /locus_tag="VFO88_09300"
     CDS             complement(5507..>6238)
                     /gene="ftsY"
                     /locus_tag="VFO88_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET9545763.1"
                     /translation="RLEERRPATQAELVEGLEEVLTDLVSPAGEARLGLGGRPSVVLV
                     VGVNGTGKTTTIGKLASRLAESGRSVILAAADTFRAAAEEQLEIWARRAGADFVGSER
                     GADPAAVAFDAIAAAEARGRDVVVVDTAGRLHTQTNLMDELAKIRRVIAGRLEGAPHE
                     TLLVVDATTGQNGLQQARLFGDAVDVTGVALTKLDGTAKGGIAVAIAHELGIPVKLVG
                     VGETLDDLQPFDAGDFVRALVGSDG"
BASE COUNT          842 a         2305 c         2217 g          874 t
ORIGIN      
        1 ccgcgccaag ctctactacc tgcgcgggaa ggtcggcaag aaggctcgcg tccgcgagaa
       61 gcgctgagcg cgggcgggcg ccgcgccggc ggggcgtagg ggtggacgcg gcccgcgcgc
      121 tccacgtgcg cacgcgcccg cgcgcgggcg caatccccct aggggatcgc gagcagcccg
      181 gtacgaacgc tatcgaccga aagcgcgccg ttcgtcacgg agccgtgccg aatgcgtgca
      241 aagcgtgccc ccagccgccc gagccgcgtc ccccgaggcc cgccccgccg gcgcgcagcc
      301 cgcccgcgct cagcgcttct cgcggacgcg agccttcttg ccgaccttcc cgcgcaggta
      361 gtagagcttc gcgcggttca cgtctccgat cgcctgcacc tcgatcttct cgatcttcgg
      421 cgagtggagc gggaacgtcc gctcgacgcc gaccccgaag gagctcttcc ggacggtgaa
      481 cgtctcgcgc gccccggcgc cctggcgctt gatcacgatg ccctcgaaga cctgcacgcg
      541 ccgccgctgc ccctcgatga cctggaagtg cacgcgcacg gtgtcccccg ccttgaactg
      601 cgggagcccg tcacgcagct ggcgctgctc gatcaggtcg atggggctgg ccatggcaaa
      661 gaggtcggcc ggggagacgg ccggcgggaa actttagcga aagccgattc gctgcggggg
      721 ccagtagacg gcgaagacct cgccgatgat gttgccgcgc ggcaccggcc cccacacgcg
      781 gctgtcgcac gactggttgc ggttgtcccc gagcatgaag tagtgcccct cggggatcgt
      841 ctgctcgggg aagctcgagc cgtcgcgctg gctcggatcg aggtacggct cgtcgagcgg
      901 ctcgccgttg atgaggatgc ggccgttctg ctcccggatc cgctcgccgg gcagcccgac
      961 gagacgcttc acgaacgtct cgcccgcgag gccgaagtcg cagcgggacg cctccggcgg
     1021 ggtcttgaag acgatcacgt cgccgcgttc cggatccttg aagtggtaga tgaagcggtt
     1081 cgcgagcacc cggtcggaga agctcgactg gcacccgacg cccggctccg cgcagtgcag
     1141 cgtcggctcc atcgacgagg acggaatccg gtatgggttc accacgtacg ccttgatcgc
     1201 gagcacgacg atgatcgcga ccgcgatcgt cacgacccag tcgacgacgg cgcgccacgg
     1261 ctccgggatc cacccggtaa gccggtcgac ggggcccctc accgtgcgcc gaccagtccg
     1321 ccgccggacg agcgcgtccg gctctgctcg cggcgccact cctcgatccg gccgtggtcg
     1381 ccggagagga gcacgtcggg cacgcgccag ccgcggaagt ccggcggacg cgtgaagtgc
     1441 ggatactcga gcccgccctc cagggcggcg gagaagctct cgacggcgcc gctctccgcg
     1501 cgcagcgccc cgggcagccg ccgggcgacc gcgtcgagga cgaccatcgc aggcagctcg
     1561 ccgccggaga ggacgtacgg gccgatcgag accgcgtcgg tcgcgagatg gcgggccacg
     1621 cgctcgtcga agccctcgga gcgcgcggag aggagcgtga ggcgctcctc ggccgcgagc
     1681 tcctccacga gctcctggtc gagcgggcgc ccctgcgggg tgagcgcgac cacgcggcgg
     1741 tcgctccagt cgcctgcgta gacggcgtcg agcgccgcgt ggacgacgtc gacgcgcagc
     1801 accatcccgg cgccgccccc gtagggctcg tcgtccacct ggcccgcccg gagcggcgtg
     1861 aagtcccggt agctccacag ccgcaggtca agctcggagc cgagcacggc ggccagcggc
     1921 cggtgctcgg tcatccacga gaacgcgtgc gggatgaggg tgaagacgtc gatctgcatt
     1981 gcgcggaaga ggttagccgc cgggggcgcg ggcgttccgt gtgacccgcc cgggccggtc
     2041 acaccgcgaa cccccgggcg acgaggatcc ggccgcccgc gagatcgacc tcgcggacgc
     2101 agtcctcgac gagcgggagc gcgacgcccg agtccaggcg cagcacgtcg ttcgccacgc
     2161 cgggctcgac ctcctgcacg ctgccgagca cctccccccc gtcctcctcc accgcgagcc
     2221 cgacgagctg gaaggcgtag tagcttccct cctccggctc cggcagctcg tccacgggca
     2281 gctcgagcgc cgcgccgcgc tcgaccgccc ggtcgagccg cacgacgagc cgtccgcggc
     2341 cggcgcgctt cgactcgacg acggtcgccg gctcgcgtcc gacgtacacg cgcgcgcccc
     2401 gggcgaagcg ttcgggcgcc tcgctcgcgt gctcgacgac gaagtcgccg cgcaggccgt
     2461 gcggccgccc gacgaacccg accgcgaccc acccgcggtc agccaacgat ctcgagcagg
     2521 cgccgctcgt ccgccttcgc cccgctcgcg cggacgagcg tgcggagcgc gcgcgcgatc
     2581 cgcccgccct tcccgatcac cttgccgagg tcggcctcgg cgacgtggag ctcgaggacg
     2641 atcgctccct cgcgctcggt ccgctcgacg cgcacggcgt ccggctcgga gacgaggctc
     2701 cgcgcgaggt ggacgagcat ctcctcgacg ggcgtcgcgg ccacggctgc gcgggagatc
     2761 agctcggcgg gccggaggcc tgcgccttga gcaggcgccg caccgactcg ctcggctgcg
     2821 cgcccttctc gatccacgcc tggatcttgt cggtgtcgag atcgatcgtg gaggggtcgg
     2881 tctgggggtt gtagcgcccg acgatctcga tgaagcggcc gtcgcgcggc gagcgcccgt
     2941 cggccacgac gacccggtag atcgggttct tcttcgagcc cacccgggcc agcctgattc
     3001 tcgttgccat ccgtctcctt cgctagcgcc gcacctgctg ggcgacgatc gactgcaggt
     3061 tgggcatttt gccttgcccg agctgcttca tcatcttctg catctgcttg cgcgcctgca
     3121 ggagctggtt cacctgctgg acgctcgtac cgctcccgcg cgcgatccgg gcccgccgcg
     3181 agccgttgat gatctccggc cgcgcgcgct cgtcggcggt catcgagagc acgatcgcct
     3241 cgacgcgcgc gaggtgccgc tcgtcgacgt tcacgccctt catctgcttg ccgaggccgg
     3301 ggatcatgcc catgaccgac tggagcgggc ccatcttccg catcatccgc tgcgcctgga
     3361 ggaagtcgtc gaacgtgaac tggccggcgc ggaggcggcg ctccatcgcc tcctgatcgt
     3421 cctcctcgac ggcggcctcc gctttctcga tgagcgtgag gacgtcgccc atcccgagga
     3481 tccgcgaggc catccggtcg ggatggaact cctccagctg gtcgagcttc tcgccggtcg
     3541 agacgagctt gatcggcttc ccggtcaccg ccttcacgga gagcgccgcg ccgccgcgcg
     3601 cgtcgccgtc gagcttcgtc aggacgacgc cgtcgaagtc gatccgctcg gcgaacgcct
     3661 ccgcggtcgt gaccgcgtcc tggccggtca tcgcgtcgag cacgaggagg acgttcagcg
     3721 gcttgacggc cgcgcggacg gcggcgagct cgtccatgag ctcctcgtcg atgtgcagcc
     3781 ggccggccgt gtcgacgacg aggacgtcgc gcccctgcgc gcgcgcctgc tcgagcccgt
     3841 gcttcgcgac cgcgacggca tccttcgtct gccggtcgag gtagaccggg atctggatct
     3901 gcttgccgag ctgctcgagc tggtcgatcg cggccgggcg ctggaggtcg gcggcgacga
     3961 gcgcgggcgt cttcccctgc ttccgcaggt ggagcgccag cttcgcggac gcggtcgtct
     4021 tccccgaccc ctgcaggccc gcgaggagga tgacggtcgg cggccggccg gcgaacgcga
     4081 gcttcgaccc gcccgagccc atgagcgcgg tcagctcctc gtggacgatc ttgatcacct
     4141 cctggccggg cgtgaggctc ttcgtcacgt ccgcgccgag cgagcgctcc ttcaccgtgg
     4201 cgacgaaccc cttgacgacc tggaagttga cgtccgcctc gaggagcgcg agccggatct
     4261 cgcgggccgc gcgcgacagg tcctcctcgg acaggcggcc gcggccgcgg aggtcgccga
     4321 gggtcagctg cagcttgtcg gagaggcttt cgaacatgcg tgggaggttc ggagggcgcg
     4381 ggacccggat aggacacgcg caggcgggtc agtgtagccc gccggtcgtg caacccaccc
     4441 caaggaggaa tgcgatggcg gacgtcgcca aggtcgtgac ggtgatcggg aactccccgg
     4501 agagcttcgc ccaggccgcg caggtcgcgg tcgaggaggc gtcgaagacg ctacgcggga
     4561 tcacgggggc cgatgtgatc tcgatgagct gcgaggtggc cgacggccgg atcacgacgt
     4621 accggacgac cgtcaacatc gccttcgcgc tcgagcgcgc gtagcgcggc ggtctagctc
     4681 gactgggcgg tcggccgcac gaggacctcg ttcacgtcca cgtgcggcgg ctgcccgacc
     4741 gcatagagga cggcgcgggc gacgtcctcc ggctcgagcg cgtccgtcac cgggtcgtcg
     4801 aagaacgggg tcgcgacggt gccgggctcg atcagcgtca cgcggacgcc ggtgccgtcg
     4861 agctcctgcc gggcggcctc gcccatcgcg gtcaccgccc acttcgtcgc cgagtagagc
     4921 gagcccgaaa gcacgcggcg gcccgcgacg gagccggtga ggagcaggtg gccgcgggac
     4981 gccttgaggg ccgggaacgt ggcgcgcagc gtgagcgcgg cgccgtagat gttcgtgagc
     5041 accatcgcgc gccagtgctc gggcgtcgag gcctcgaacc cgcgcgtcgc gccgaagccc
     5101 gcgttcgcga agacgacgtc gatcgagccg aacgcgtcga gcgcggcgtc ggcgagcgcg
     5161 cggacgtcgt cccactcggt cacgtcgcag cgcacggcgc gggcgcgctc ttcgccgccg
     5221 agctcggccg cgaggccctc gagccgctcg agccggcgcg ccgcgaggac gagccggaag
     5281 ccgtcggcgg cggccaggcg cgcggtggcc tcgccgatcc cgctcgacgc gcccgtgatc
     5341 acgagcacgc ggtcagccat cggacatccc cgttccccgc tcgccgtcac acaaccgaca
     5401 cggtttcgtc gcgggtcctt cgcaaacggt cacagacacg tgcggttctc ctcgatacgc
     5461 tttcactagt gggcggtggg ggtcttccct catcccgtgc gcgcgtctat ccgtcggagc
     5521 cgacgagcgc gcgtacgaag tcccccgcgt cgaacggctg gaggtcgtcg agcgtctccc
     5581 ccacgccgac gagcttcacc gggatcccga gctcgtgcgc gatcgcgacg gcgatgccgc
     5641 ccttcgcggt gccgtcgagc ttcgtcagcg cgaccccggt gacgtcgacc gcgtcgccga
     5701 agagccgcgc ctgctggagc ccgttctggc ctgtcgtcgc gtccacgacg agcaaggtct
     5761 cgtgcggcgc gccctccagc cgaccggcga tcacgcgccg gatcttcgcg agctcgtcca
     5821 tgaggttcgt ctgcgtgtgg agccgccccg ccgtgtcgac gacgacgacg tcgcgcccgc
     5881 gcgcctccgc ggcggcgatg gcgtcgaagg cgaccgcggc gggatcggcg ccgcgctccg
     5941 agccgacgaa gtccgcgccc gcgcgcctcg cccagatctc gagctgctcc tcggcggccg
     6001 cgcggaacgt gtccgcggcg gcgaggatga cggagcggcc cgattcggcg aggcgcgacg
     6061 cgagcttgcc gatcgtcgtc gtcttccccg tcccgttcac gccgacgacg aggacgacgc
     6121 tcggccgccc gcccagcccg agccgcgcct cgccggccgg tgagacgaga tcggtcagca
     6181 cctcctcgag cccctcgacg agctcggcct gcgtcgccgg gcgccgctcc tcgagccg
//
LOCUS       DASTFC010000314         7665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1145661,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000314 DASTFC010000000
VERSION     DASTFC010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145661"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFO88_09305"
     CDS             complement(<1..549)
                     /locus_tag="VFO88_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET9545764.1"
                     /translation="MGETAPRQLWGGETAKAVDNFPVSGEPMPAPVVRGLGRIKAAAA
                     RVNAELGLLDADLAERIAAAGDRVAAGEFDDQFPIDVFQTGSGTSSNMNANEVIASLA
                     GEGAHPNDHVNLGQSSNDVFPSAVHLAALGEIQSDLLPALGRLGEALEAKADEFADAV
                     KSGRTHLMDAVPVTLGQEFAGYA"
     gene            802..1146
                     /locus_tag="VFO88_09310"
     CDS             802..1146
                     /locus_tag="VFO88_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545765.1"
                     /translation="MRIERRPLLDARRVPRLAFAPRLHPSLHEALAALDDPDVPIAET
                     YRRLTAFARRERLFRPSYEHVRRLIHALRRLRSAKGRGGTAHVLLQVAYNVRPPTALV
                     DHLLDVGPAGPT"
     gene            complement(1174..1380)
                     /locus_tag="VFO88_09315"
     CDS             complement(1174..1380)
                     /locus_tag="VFO88_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545766.1"
                     /translation="METKLRIECLGCGEARVVPAAEPGRQLHTGECHRCGYVGWAPSA
                     ALSEPMRRRIRERPVDRRAAVYVL"
     gene            complement(1447..2451)
                     /locus_tag="VFO88_09320"
     CDS             complement(1447..2451)
                     /locus_tag="VFO88_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9545767.1"
                     /translation="MSQVYDITVIGAGPVGLATAYYAGHREATVCIVESLEQLGGQVA
                     AVYPEKHVFDVAGHPKVQGQALADLCAEQGLQFGADVLLGEEVKDLTRIGQNGEEVLQ
                     LTTHSGKTVRSRVLIVTAGHGAFEPRKLGVDGVDAWEGKGLHYFVRQKDAFRDKSCVI
                     VGGGDSALDWTLGLQDTAALPITLIHRRERFRALESSVTAARRLEEEGKVRIVVPAEV
                     REIDGNGTVERVLVENTSTGETEEIACDALITLLGFHSHLGSIADWGLELHGKRQILV
                     DPATMETSLHGVYAAGDVAGYEGKITLITIGFGEAAIAANNAIAKIRGEKVQPKYSTD
                     "
     gene            complement(2492..2893)
                     /gene="erpA"
                     /locus_tag="VFO88_09325"
     CDS             complement(2492..2893)
                     /gene="erpA"
                     /locus_tag="VFO88_09325"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET9545768.1"
                     /translation="MITITQSAAQKIQALLAEEPESDANVLRVAIQGGGCSGFQYALG
                     FDRGPQDGDNEIEMHGVKVVIDPFSAPYLTGSEIDYVDALMGAGFAINNPNVQAACGC
                     GSSFQAKEGVEGEPAGATTHAGGACGSGCSH"
     gene            3039..3410
                     /gene="ndhC"
                     /locus_tag="VFO88_09330"
     CDS             3039..3410
                     /gene="ndhC"
                     /locus_tag="VFO88_09330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9545769.1"
                     /translation="MPLAALSDWFPFLVYALIAFAIPASMIVMSFVFAARSRRSRARA
                     MPFESGVSQGPPPEQRFTVSFYLTAMLFIVFDIEIVFLFPLAVVLGDVGWLAVGAFGF
                     FIAMLVIPFVYDWKKGALEWR"
     gene            3401..4000
                     /locus_tag="VFO88_09335"
     CDS             3401..4000
                     /locus_tag="VFO88_09335"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET9545770.1"
                     /translation="MAVGKKRLADYGLNTERILMEKHGPKRDEPPAFEAELGNLEERL
                     MLTTVEKALAWAQRGSMWPDTFGLACCAIEMMSIVSSRYDVSRFGMEVFRSSPRQADL
                     LIVSGRVSHKMSAPLRQIYDQMLEPKWVMAMGACASSAGMFNNYAVLQGVDKICPVDI
                     YVPGCPPRPEAVLEGIVRLHERIKAGVPPAYELRKAAQG"
     gene            3997..4578
                     /locus_tag="VFO88_09340"
     CDS             3997..4578
                     /locus_tag="VFO88_09340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9545771.1"
                     /translation="MTSYADAPGFVEEREALGEVTVVVERESLREAAEWFRDEHGFNF
                     LADLSTADYLGWGAKGVAGYWGSPPPSPQTRDWTPPARDLNAPGSWGLQKLPEPKPKR
                     FSVSYHLLALGPDPRRLRLQVWCDDGEPVPSVIRVWPAADFFEREAWDMMGIAIDGHP
                     YLKRILMDDDWEGHPQRKDYPLGGEPVRFSDED"
     gene            4583..5863
                     /gene="nuoD"
                     /locus_tag="VFO88_09345"
     CDS             4583..5863
                     /gene="nuoD"
                     /locus_tag="VFO88_09345"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HET9545772.1"
                     /translation="MAIAPARDPLTNRRPIYEGSRIPERVPTILEVDPVLREEGDILE
                     INFGPNHPSTHGVLRLIIQLNGERVVGLEAVIGYLHTGFEKNMEAKTYWKAITYAPRM
                     DYVAYQANEYVFVAAIEKLLQMEIPPKATWMRTLMLELNRLHSHLVWLGTGALELGAV
                     SMFWYCFRERDAILDLFEMVGGARMHTRYFQAGGLAEDIPIGFYEECQRFCDTMPARI
                     DEYEGMLDGQAIFLERTKGVGLLSAADAVALGQSGPVLRASGVDWDIRRSEPYLAYDE
                     IDFDVPVEADGDVYARYRVRMREMRESVRIVQQCLDGMPEGPWIADDRKVVLPPRHEL
                     HTSMESLIHHFKLVTEGYRVPEGEVYVPIESPRGELGCYLVSDGGPRPWRVRFRTPSY
                     VVLQAAPTAMSNALIADLIAIVASLDNVMGEADR"
     gene            5887..6351
                     /locus_tag="VFO88_09350"
     CDS             5887..6351
                     /locus_tag="VFO88_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET9545773.1"
                     /translation="MQEVAARYPSSLSAIMPALHLAQDRYGWLPPEALREVAEALDLT
                     PAYCYAVASFYDMYHLEPVGRHEIAVCTNVSCALAGAQDVLAAFERELGVEAGGTTED
                     GEFSLSSFECLGGCGWATVVAVNWRYREHVSAGDVPGIVAELRGGEAAGDDA"
     gene            6341..>7665
                     /gene="nuoF"
                     /locus_tag="VFO88_09355"
     CDS             6341..>7665
                     /gene="nuoF"
                     /locus_tag="VFO88_09355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET9545774.1"
                     /translation="MTPSGLHEVLLRHAGERDLTKRAEYEAVGGYRALATALRTDPGE
                     LVESLLASGLRGRGGAGFPMGRKASFIPKPDQTPNPIYLTVNADESEPGTFKDRELML
                     RIPHAVIEGIVITCHAIGAETAFVYLRGEYATEFDVMAAALDEAQEAGYVGPGIAGTD
                     TSVTIVIHRGAGAYICGEETALLESLEGKRGQPRSKPPFPAISGVYASPTLINNVETI
                     ATVPTIVGMGGEEYAKLGVENSAGTRLFSLSGNVVKGGNYELELGTPLRELIYDLGGG
                     IPDGRALKAVIPGGSSVPVLTADEVDTALDFDAMAQAGTMLGSGAVIVIDERACMVQL
                     GLRVAQFYMHESCGKCTPCREGTRWMVQILRKLEAGEAEQGELDLLLNVCDRILGNCL
                     CPLGDAAAMPIASYVAKFREEYQRHIDEGGCPFGGESSLEGVVAPVDQH"
BASE COUNT         1062 a         2759 c         2688 g         1156 t
ORIGIN      
        1 cgcgtagccc gcgaactcct ggccgagcgt gacgggcacg gcgtccatca ggtgcgtccg
       61 gccggacttg accgcgtcgg cgaactcgtc ggccttcgcc tcgagcgcct ccccgagccg
      121 gccgagcgcc gggaggaggt cgctctggat ctcgccgagc gccgccaggt ggacggccga
      181 cgggaagacg tcgttcgagc tctggccgag gttcacgtgg tcgttcgggt gggcgccctc
      241 gcccgcgagc gaggcgatca cctcgttcgc gttcatgttc gagctcgtgc ccgagccggt
      301 ctggaagacg tcgatcggga actggtcgtc gaactcgccg gcggcgaccc ggtcgcccgc
      361 ggccgcgatc cgctcggcga ggtccgcgtc gaggagaccg agctcggcgt tcacgcgcgc
      421 cgcggcggcc ttgatgcgcc cgagcccgcg gacgacgggc gccggcatcg gctcgccgga
      481 gacgggaaag ttgtcgaccg ccttcgcggt ctccccgccc cagagctgtc gcggcgccgt
      541 ctcgcccatc gcctccgagt ctacccgggc cacgacttgg gttctcccgt cgtgtcgcgc
      601 ggccggtacc gcccgggccg cgcgggcgat cctccctcgc cggcctccag cggtcctcgc
      661 accgggttgc tcgtgacact ccgttacaag cacacgagac cgtccagcga ccacgcggag
      721 atcctcgtga cagagtgtca cagggggcgt tggcacgctt tcggcacacg gcggtcgggg
      781 agcgggcggc aaggtgggtc catgcggatc gagcggcggc cgcttctcga cgcgcggcgg
      841 gtgccgcggc ttgcgttcgc gccgcggctg cacccctcgc tccacgaggc gctcgcggcg
      901 ctcgacgatc ccgacgtgcc gatcgccgag acctaccgtc gcctcaccgc gttcgcccgt
      961 cgggaaaggc tcttccgccc gtcgtacgag cacgtccgcc ggctgatcca tgcgcttcgt
     1021 cggctccgca gcgcgaaggg acgcggaggc accgcgcacg tgctgctcca ggtcgcctac
     1081 aacgtccgtc cgccgaccgc gctcgtcgac cacctgctcg acgtcgggcc cgccgggccg
     1141 acctgacgcc gccgcacggg accggcccgg cggctagagg acgtagacgg cggcccggcg
     1201 gtcgaccggg cgctcgcgga tccgccggcg catcggctcg ctcagcgccg ccgagggcgc
     1261 ccagccgacg tagccgcacc ggtggcactc accggtgtga agctgccgtc cgggctccgc
     1321 cgcgggaacc acacgcgcct cgccgcagcc gaggcactcg attcgaagct tcgtctccac
     1381 gtcccagttg tcgggcgtcg cgccgaacgc gctgaacccc gaacgggtga agcacggccg
     1441 gaaccgtcag tcggtcgagt acttgggctg gaccttctcg ccgcggatct tcgcgatcgc
     1501 gttgttcgcc gcgatggccg cctcgccgaa gccgatcgtg ataagcgtga tcttcccctc
     1561 gtagccggcc acgtcccccg ccgcgtagac gccgtggagc gaggtctcca tcgtcgcggg
     1621 atcgacgagg atctgccgct tcccgtgcag ctcgagcccc cagtcggcga tcgagccgag
     1681 gtgggagtga aatccgagca gcgtgatcag cgcgtcgcac gcgatctcct ccgtctcgcc
     1741 ggtggaggtg ttctcgacga gcacgcgctc gaccgtcccg ttcccgtcga tctcgcgcac
     1801 ctcggcgggg acgacgatcc ggaccttccc ctcctcctcg agccgccgcg ccgcggtgac
     1861 gctcgactcg agcgcgcgga agcgctcgcg ccggtggatc agcgtgatcg gcagcgcggc
     1921 cgtgtcctgc agcccgagcg tccagtcgag cgccgagtcg ccgcccccga cgatcacgca
     1981 gctcttgtcg cggaacgcgt ccttctgccg gacgaagtag tggagcccct tgccctccca
     2041 cgcgtccacg ccgtcgacgc cgagcttccg cggctcgaag gctccgtggc ccgcggtcac
     2101 gatcagcacg cgcgagcgca cggtcttccc cgagtgggtc gtgagctgga gcacctcctc
     2161 gccgttctgg ccgatgcgcg tcaggtcctt gacctcctcg ccgagcagga cgtccgcgcc
     2221 gaactggagc ccctgctccg cgcagaggtc ggcgagcgcc tggccctgca ccttcgggtg
     2281 cccggcgacg tcgaagacgt gcttctccgg gtagaccgcg gcgacctggc cgccgagctg
     2341 ctcgaggctc tcgacgatgc agacggtcgc ctcgcgatga ccggcgtagt acgccgtcgc
     2401 aagccccacc ggcccggcgc cgatgaccgt gatgtcgtag acctggctca cgccgggaac
     2461 gctaccgcct cgtgcgcgtc cgcaatccgc gctagtgcga gcagccggag ccgcacgccc
     2521 cgccggcatg cgtggtcgcg ccggccggct cgccctcgac gccctccttc gcctgaaagg
     2581 aggagccgca gccgcaggcc gcctggacgt tcgggttgtt gatcgcgaac ccggcgccca
     2641 tgagcgcgtc cacgtagtcg atctcggagc ctgtcaggta cggcgcgctg aacggatcga
     2701 tcacgacctt gacgccgtgc atctcgatct cgttgtcgcc gtcctgcggg ccgcggtcga
     2761 agccgagggc gtactggaag cccgagcagc cgccgccctg gatggccacg cggagcacgt
     2821 tcgcgtcgct ctccggctcc tccgcgagga gggcctggat cttctgcgcg gcgctttgcg
     2881 tgatcgtgat catcctgctt cggagtgtag cagcgtcctt cccggtcgca ggccgccgct
     2941 tgtgacgcct gttgcgccac ggaaaagacc tgctcagggc catatttctc accgacggcc
     3001 gtactccttt gtgacgggtt agtatgcggg cggttgccgt gccgctcgca gcgctctcag
     3061 actggtttcc gttcctcgtc tacgcgctga tcgcgttcgc gatccccgcg tcgatgatcg
     3121 tgatgtcgtt cgtcttcgcc gcgcggtcga ggcgcagccg cgcgcgcgcc atgccgttcg
     3181 agtccggcgt gtcccaggga ccgccgcccg agcagcggtt cacggtcagc ttctacctca
     3241 cggcgatgct cttcatcgtc ttcgacatcg agatcgtgtt cctcttcccg ctcgccgtcg
     3301 tcctcgggga cgtcggctgg ctggcggtcg gcgcgttcgg gttcttcatc gcgatgctcg
     3361 tgatcccgtt cgtctacgac tggaagaagg gggcgctcga gtggcggtag ggaagaagcg
     3421 cctcgccgac tacggcctga acaccgagcg gatcctcatg gagaagcacg ggccgaagcg
     3481 cgacgagccg cccgccttcg aggccgagct cggaaacctc gaggagcgcc tcatgctgac
     3541 cacggtcgag aaggcgctcg cgtgggcgca gcgcggctcg atgtggcccg acacgttcgg
     3601 cctcgcgtgc tgcgcgatcg agatgatgtc gatcgtctcg tcgcgctacg acgtctcgcg
     3661 cttcgggatg gaggtcttcc gctcctcgcc gcgccaggcc gacctcctga tcgtctccgg
     3721 gcgcgtgtcg cacaagatgt cggcgccgct gcgccagatc tacgaccaga tgctcgagcc
     3781 gaagtgggtc atggcgatgg gcgcctgcgc gagctcggcc gggatgttca acaactacgc
     3841 cgtgctccag ggcgtggaca agatctgccc ggtcgacatc tacgtgcccg gctgcccgcc
     3901 gcggcccgag gccgtgctcg aggggatcgt ccggctgcac gagcgcatca aggccggcgt
     3961 cccgcccgcg tacgagctgc gcaaggcggc gcagggatga ccagctacgc cgacgcgccc
     4021 ggcttcgtcg aggagcggga ggcgctcggg gaggtgacgg tcgtcgtcga gcgcgagagc
     4081 ctgcgcgagg cggcggagtg gtttcgcgac gagcacggct tcaacttcct cgccgacctc
     4141 tcgaccgccg actacctcgg ctggggcgcg aagggcgtcg ccggctactg gggctcgccg
     4201 ccgccctcgc cgcagacgcg cgactggacg ccgcccgcgc gcgacctgaa cgcgcccggg
     4261 tcgtggggcc tccagaagct ccccgagccg aagccgaagc gcttctccgt ctcgtaccac
     4321 ctgctcgcgc tcgggcctga cccgcgccgc ctccgtctcc aggtctggtg cgacgacggc
     4381 gagccggtgc cgagcgtgat ccgcgtctgg cccgcggccg acttcttcga gcgcgaggcc
     4441 tgggacatga tggggatcgc gatcgacggc cacccgtacc tgaagcggat cctcatggac
     4501 gacgactggg agggccaccc gcagcggaag gactatccgc tcggcggcga gcccgtccgc
     4561 ttctcggacg aggactgacg gcatggccat cgcccccgcc cgcgacccgc tcacgaaccg
     4621 tcgcccgatc tacgagggga gccggatccc ggagcgcgtg ccgaccatcc tcgaggtcga
     4681 ccccgtcctc cgcgaggagg gcgacatcct cgagatcaac ttcggcccga accacccgtc
     4741 cacgcacggc gtgctccgcc tcatcatcca gctgaacggc gagcgggtcg tcgggctcga
     4801 ggcggtgatc ggctacctcc acaccggctt cgagaagaac atggaggcga agacgtactg
     4861 gaaggcgatc acgtacgcgc ctcgcatgga ctacgtcgcg taccaggcga acgagtacgt
     4921 cttcgtcgcg gcgatcgaga agctcctgca gatggagatc ccgcccaagg cgacgtggat
     4981 gcggacgctc atgctcgagc tgaaccggct ccactcgcac ctcgtctggc tcggcaccgg
     5041 ggcgctcgag ctgggggcgg tctcgatgtt ctggtactgc ttccgcgagc gggacgcgat
     5101 cctcgacctc ttcgagatgg tcggcggggc ccgcatgcac acgcgctact tccaggccgg
     5161 cgggctcgcc gaggacatcc cgatcggctt ctacgaggag tgccagcgct tctgcgacac
     5221 gatgcccgcc cggatcgacg agtacgaggg gatgctcgac ggccaggcga tcttcctcga
     5281 gcggacgaag ggcgtcgggc tcctgtccgc cgcagacgcg gtcgcgctcg ggcagtccgg
     5341 cccggtgctg cgtgcgtcgg gggtcgactg ggacatccgc cgcagcgagc cgtacctcgc
     5401 ctacgacgag atcgacttcg acgtgccggt cgaggccgac ggcgacgtct acgcgcgcta
     5461 ccgcgtccgc atgcgcgaga tgcgggagtc ggtgcggatc gtccagcagt gcctcgacgg
     5521 gatgcccgag gggccgtgga tcgccgacga ccgcaaggtc gtcctcccgc cccggcacga
     5581 gctgcacacg tcgatggaga gcctcatcca ccacttcaag ctcgtcaccg agggctaccg
     5641 cgtgcccgag ggggaggtct acgtgccgat cgagtcgccg cgcggcgagc tcgggtgcta
     5701 cctcgtctcg gacggcgggc cgaggccgtg gcgggtgcgg ttccggacgc cctcgtacgt
     5761 cgtcctccag gcggccccga ccgcgatgtc gaacgcgctc atcgccgacc tgatcgcgat
     5821 cgtcgcgtcg ctcgacaacg tcatggggga ggccgaccgc tgagcgcgcg cacgctctac
     5881 gacgagatcc aggaggtcgc ggcgcgctac ccgtcgagcc tctcggcgat catgcccgcg
     5941 ctccacctcg cgcaggaccg ctacggctgg ctcccgcccg aggcgctccg cgaggtggcg
     6001 gaggcgctcg acctgacgcc ggcgtactgc tacgcggtcg cgagcttcta cgacatgtac
     6061 cacctggagc cggtcgggcg gcacgagatc gcggtctgca cgaacgtctc gtgcgcgctc
     6121 gcgggcgcgc aggacgtcct cgccgcgttc gagcgcgagc tcggcgtcga ggcgggcggg
     6181 acgacggagg acggcgagtt ctcgctctcc tccttcgagt gcctcggagg ctgcgggtgg
     6241 gcgacggtcg tcgccgtgaa ctggcgctac cgcgagcacg tgagcgcggg cgacgtgccg
     6301 gggatcgtcg ccgagctgcg cggcggggag gcggccggcg atgacgccta gcgggcttca
     6361 cgaggtcctg ctccgccacg cgggcgagcg cgacctgacg aagcgcgccg agtacgaggc
     6421 ggtcggcggc taccgggcgc tcgcgacggc cctgcgcacc gacccgggcg agcttgtcga
     6481 gagcctgctc gcctccgggc tgcgcggccg cggcggcgcc ggcttcccga tgggccgcaa
     6541 ggcgagcttc atcccgaagc ccgaccagac gccgaacccg atctacctca cggtcaacgc
     6601 cgacgagtcc gagccgggca cgttcaagga ccgcgagctc atgctccgga tcccgcacgc
     6661 ggtgatcgag gggatcgtca tcacgtgcca cgcgatcggc gccgagacgg cgttcgtcta
     6721 cctgcgcggc gagtacgcga cggagttcga cgtgatggcc gcggcgctcg acgaggcgca
     6781 ggaggcgggc tacgtcggcc ccggcatcgc cgggacggac acgagcgtca cgatcgtcat
     6841 ccaccgcggc gcgggcgcgt acatctgcgg cgaggagacg gcgctcctcg agtcgctcga
     6901 ggggaagcgc ggccagccgc gctcgaagcc gccgttcccg gccatctccg gcgtctacgc
     6961 gagcccgacg ctcatcaaca acgtcgagac gatcgcgacc gtcccgacga tcgtcggcat
     7021 gggcggggag gagtacgcga agctcggcgt cgagaactcg gccggcacgc ggctcttctc
     7081 gctctccggc aacgtcgtga agggcgggaa ctacgagctc gagctcggga cgccgctccg
     7141 cgagctcatc tacgacctcg gcggcgggat ccccgacggg cgcgcgctca aggccgtgat
     7201 ccccggcggc tcctcggtgc cggtgctgac ggcggacgag gtggacacgg cgctcgactt
     7261 cgacgcgatg gcgcaggcgg ggacgatgct cggctccggc gcggtgatcg tgatcgacga
     7321 gcgcgcctgc atggtgcagc tcggcctgcg cgtggcgcag ttctacatgc acgagtcctg
     7381 cgggaagtgc acgccgtgcc gggaggggac gcgctggatg gtgcagatcc tccgcaagct
     7441 cgaggccggc gaggcggagc agggcgagct cgacctcctg ctgaacgtct gcgaccggat
     7501 cctcggcaac tgcctctgcc cgctcggcga cgcggccgcg atgccgatcg cgagctacgt
     7561 ggcgaagttc cgcgaggagt accagcgcca catcgacgag ggcggctgcc cgttcggcgg
     7621 cgagtcctcg ctcgagggcg tcgtcgcgcc cgtcgaccag cacca
//
LOCUS       DASTFC010000315         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_931183,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000315 DASTFC010000000
VERSION     DASTFC010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931183"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFO88_09360"
     CDS             <1..730
                     /locus_tag="VFO88_09360"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9545775.1"
                     /translation="ANWVYVYGHWPVIAAAAVLLYAFRRERYVLLRNAMFISGLCGFA
                     FFAFFPVAPPRLVDPSLVDTVTLYSEGYRTLQPPSLTNQFAAMPSLHAGWNLLVGIVV
                     FGATANLLLRALAVAMPAAMLFAVVATANHYVLDVVVGVLLVLAGLAVALRMQPRTLR
                     SGESEAPTEGRLGRPTLRRRPSLGQLAREGPSGRARRRAGARGGRAPVPRSARAPSPQ
                     DRRPPAPVGPVEAREPVLPASRSR"
     gene            831..1178
                     /locus_tag="VFO88_09365"
     CDS             831..1178
                     /locus_tag="VFO88_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545776.1"
                     /translation="MAAGGATTVCSRSWRLLEPFRAARGIRVVHSVGSERQLARLRRR
                     FAGAGLDGVSIHERLLDAGTVRDLQAISRTILAWPVNTAARARELTALGVTGLISDRP
                     GELHAALAAEKVR"
     gene            1183..>2081
                     /locus_tag="VFO88_09370"
     CDS             1183..>2081
                     /locus_tag="VFO88_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9545777.1"
                     /translation="MPSFETLAATFAGLDGRLVAVALVFHLANFGFRSLAWRNVLRAA
                     YPDRAVPVLSIAGAYAAGVAVNAFTPARGGDLLKIALVRARIRGSSVPTIASSMGVVT
                     LFDLVVGASAIAALAALGLLPAPPGLPQLPTAPALVTEHLAVALPVAVALAAVAALLA
                     RRFRGRLAAAWAGVKAGASILGTPRRYLTEVAPLQLSAWVCRVGAVFFLLAAFGLPAT
                     LPVALIVVMAGGLSTIVPTPGGIGTQQVLLAYLLHATASTAAVVSFSLGMQAAVTTLN
                     AFLGAAALMLMFRTLRPASAVRA"
BASE COUNT          200 a          820 c          727 g          334 t
ORIGIN      
        1 cgcgaactgg gtgtacgtgt acggccactg gccggtcatc gcggcggcgg cggtcctcct
       61 ctacgcgttc cggcgcgagc gctacgtcct cctgcgcaac gcgatgttca tctccgggct
      121 ctgcggcttc gccttctttg cgttcttccc ggtcgcgccg ccccggctcg tcgacccgtc
      181 cctcgtggac acggtgacgc tctactcgga ggggtaccgc accctccagc cgccctcgct
      241 cacgaaccag ttcgcggcga tgccgagcct gcacgcgggg tggaacctcc tcgtcgggat
      301 cgtcgtcttc ggcgcgacgg ccaacctcct cctgcgggcg ctcgccgtgg cgatgccggc
      361 cgcgatgctg ttcgcggtcg tcgccaccgc gaaccactac gtgctcgacg tcgtcgtcgg
      421 agtcctgctc gtcctggccg gcctcgccgt cgcgcttcgc atgcagccac gtacactgag
      481 gagtggtgag tccgaggccc ccacggaggg acgcctcggc cgtccgaccc ttcgtcgtcg
      541 cccatcgctc gggcaactcg ctcgcgaagg cccgagcggc cgagcgcgtc ggcgtgccgg
      601 cgctcgaggc ggacgtgcac ctgtaccgcg gtcggctcga gctccgtcac ctcaagaccg
      661 caggccccct gctcctgtgg gaccggtgga ggctcgcgaa cccgttctcc cggcgtctcg
      721 ttctcgctga gctccttccc gtcgtcgggc gcgagaccga gctcgtcctc gacctgaagg
      781 ggtggagcac ccggctgagc gagcgggtcg cgacggcgct cgagccacgc gtggccgcgg
      841 gcggcgcgac gaccgtctgc tcgcggagct ggcggctgct cgagcccttc cgggccgcgc
      901 gcgggatccg cgtcgtccac tcggtcggga gcgagcgcca gctcgcccgc ctccgccgcc
      961 ggttcgccgg cgccgggctc gacggcgtct cgatccacga gcggctactc gacgcgggca
     1021 ccgtccgcga cctccaggcg atcagccgga cgatcctcgc ctggccggtg aacaccgcgg
     1081 cccgggcgcg agagctgacg gcgctcggcg tcacgggcct gatcagcgac cggccgggcg
     1141 agctccacgc ggcgctcgcg gccgagaagg tgcgctgagc ccttgccctc gttcgagacc
     1201 ctcgcggcca ccttcgcggg cctcgacggc aggctcgtcg ccgtcgcgct cgtgttccac
     1261 cttgcgaact tcggcttccg gtcgctcgcc tggcgcaacg tcctgcgcgc cgcctatccg
     1321 gaccgggccg ttccggtgct ctcgatcgcc ggcgcgtacg cggccggcgt cgcggtgaac
     1381 gcgttcaccc ccgcccgcgg cggcgacctg ctgaagatcg cgctcgtccg ggcgcgcatc
     1441 cgcggctcgt ccgtcccgac gatcgcctcg tcgatgggcg tcgtcacgct cttcgacctc
     1501 gtcgtcgggg cgagcgcgat cgcggcgctc gcggcgctcg gcctcctccc cgcgccgccc
     1561 ggcctgcccc agcttccgac ggcgcccgcg ctcgtcacgg agcatctcgc ggtcgcgctg
     1621 ccggtcgccg tcgcgctcgc cgcggtcgcc gcgctcctcg cgcgccggtt ccgaggccgc
     1681 ctcgcggcgg cgtgggccgg cgtgaaggcg ggggcgtcga tcctcgggac gccgcgccgc
     1741 tacctcacgg aggtcgcgcc gctccagctc tccgcctggg tctgccgcgt cggggcggtg
     1801 ttcttcctgc tcgcggcctt cgggctgccg gccacgctgc cggtcgcgct catcgtcgtc
     1861 atggcgggag ggctctcgac gatcgtcccg acgcccggcg ggatcgggac gcagcaggtg
     1921 ctgctcgcgt acctcctgca cgcgaccgcg tcgaccgcgg cggtcgtgtc gttctcgctc
     1981 gggatgcagg cggccgtgac gacgctgaac gccttcctcg gcgccgcggc cctcatgctc
     2041 atgttcagga cgctccggcc ggcgagcgcc gtgcgggcgc a
//
LOCUS       DASTFC010000316         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1145775,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000316 DASTFC010000000
VERSION     DASTFC010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145775"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFO88_09375"
     CDS             complement(<1..351)
                     /locus_tag="VFO88_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545778.1"
                     /translation="MIRSTALILLVLGSAFAVAPAAGKQLPVIPRGVEVGGVQVGGLI
                     TVQAEAKVQRTYDKPLHLFHEDSQWTVKPGRFGAEASIAEAVEAALDAEPNARLDLDV
                     VVRKKAVRKYVDELD"
     gene            448..1524
                     /locus_tag="VFO88_09380"
     CDS             448..1524
                     /locus_tag="VFO88_09380"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET9545779.1"
                     /translation="MIDLRSDTLTQPTPGMRRAMAEAVVGDEQKREDPTVIALEERAA
                     ELLGQEEAVYLPTASMGNQIALRLLTEPGDELLVEARAHIYRYELGGLAVHAGLALKP
                     LATEDGRLTPEQVRAAVNPPRDLHMAPTRVLALENTHNGGGGRVWPLDRYREVVAEAR
                     GHGLAVHLDGARLLNASVAAGVPAAEYGREADTVTLCLSKGLGCPLGALVATSRELAP
                     KARRLKHLFGGAMRQAGIVAAAGLYALDHHVDRLADDHANARRLVEGLAEAGMPVDPA
                     QVESNFVLVDVGSLGLDPDEAVARLRGEGVLLSFASKPGVLRAVTHLDVSEADVAAAI
                     PAIARALSGREAPVAVGLDAPTPY"
     gene            1646..3142
                     /locus_tag="VFO88_09385"
     CDS             1646..3142
                     /locus_tag="VFO88_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9545780.1"
                     /translation="MTRTPRRRIAALVAAAAALVLPAAAQAQAPPPTVADPNLEVTTV
                     ATGLAQPTGLAFIGPNDMLVNQKANGQVRRILNGELLADPVLDLAVNSQSERGLLGIA
                     THPNFFVNGWVYLFWTESTTGADTNVNGNVALLGNRVDRFVWDGSTLTFDRPITRFRA
                     AQPAVAAAPPLPAQGAAGNHNGGVVRFGPDGKLYVIVGDTGRRGWMQNLRCGPFGLET
                     IYTCPPDTPVPDDAFGGPQPDDAHFTGVIVRLNDDGTTPADNPFFDAGAAIGGEVGAN
                     IQKIYAYGVRNSYGLNFDPVSGDLWQSENGDDTFSELNRVEPGMNSGWPQIMGPLDRL
                     AEYKAIETTPPFVGLQQNRWPPANIADTPEEALGRLVTFPGSHFSDPEFSWKFEVAPA
                     GLGFVSGRALGPQYDGDLFSGGARNFLEGGHIFRFDLTGNRRKVGVDDPRIEDRVADN
                     LAKFEITESESLLFGTDFGLTTDILQGPDGALYVLSNTRSALYKISRR"
     gene            complement(3196..>4011)
                     /locus_tag="VFO88_09390"
     CDS             complement(3196..>4011)
                     /locus_tag="VFO88_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9545781.1"
                     /translation="IETDRIAEAYTFKWQNGSDDVLADLGRDGAIVAKDFAESNDLEV
                     GSPVKVASPAGEEALLEVKAIYEPPPFYPILGAVSISIETFDSLYERPRNQFVFANVA
                     GNPTDATTEQLEAGVAGFPDAKVQTKEQWITQQDEDFNQFLIMLYVLLALAVIISLFG
                     MVNTLVLTVYERTRELGMLRAVGMTKRQTRRMIRHESVITALIGAALGVPLGIFLAIL
                     VTQALSQFDLQFAVPTGWLIAFAIIAVIVGILAAIAPARRAARLNVLEALQYE"
BASE COUNT          596 a         1481 c         1391 g          543 t
ORIGIN      
        1 gtcgagctcg tccacgtact tccggacggc cttcttccgc acgacgacgt cgaggtcgag
       61 ccgcgcgttc ggctccgcgt cgagcgccgc ctccacggcc tccgcgatcg acgcctcggc
      121 gccgaagcgg cccggcttca ccgtccactg cgagtcctcg tggaacaggt ggagcggctt
      181 gtcgtacgtc cgctggacct tcgcctcggc ctgcaccgtg atgaggccgc cgacctggac
      241 gccgcccacc tcgacgccgc gcggaatcac gggcagctgc tttcccgccg ccggagcgac
      301 cgcgaacgcg cttcccagga ccaggaggat gagtgccgtg ctgcgaatca cgaaccactg
      361 atggtaggca aatttccccg catccgcgac gaaaatttac gaacagaggc ggatggcgtc
      421 ccgatgccct cccgctaggc tgggcgcgtg atcgacctgc gctcggacac gctcacccag
      481 ccgactcccg gcatgcgccg cgcgatggcc gaggcggtcg tcggcgacga gcagaagcgc
      541 gaggacccga cggtgatcgc gctcgaggag cgcgcggccg agctgctggg gcaggaggag
      601 gccgtctacc tcccgaccgc gtccatgggg aaccagatcg cgctccggct gctcacggag
      661 ccgggcgacg agctgctggt cgaggcgcgg gcacacatct accgctacga gctgggcggg
      721 ctggccgtcc acgcgggcct cgcgctgaag ccgctcgcga ccgaggacgg gcgcctcacc
      781 ccggagcagg tgcgcgccgc cgtgaacccg ccgcgcgacc tccacatggc gccgacgcgc
      841 gtgctcgcgc tcgagaacac gcacaacggc ggcggcgggc gcgtctggcc gctcgaccgc
      901 taccgcgagg tcgtcgcgga ggcccgcgga cacgggctcg ccgtgcacct ggacggggcg
      961 cgcctcctga acgcctcggt ggcggccggc gtccccgcgg ccgagtacgg gcgcgaggcc
     1021 gacacggtca cgctctgcct ctcgaagggc ctcggctgcc cgctcggggc gctcgtcgcg
     1081 acgtcgcggg agctcgcccc gaaggcacgg cgcctcaagc acctgttcgg cggcgcgatg
     1141 cgccaggccg ggatcgtcgc ggcggcgggc ctctacgcgc tcgaccacca cgtcgaccgg
     1201 ctcgcggacg accacgcgaa cgcgcgccgc ctcgtcgagg ggctcgcgga ggccggcatg
     1261 ccggtcgatc cggcgcaggt cgagtcgaac ttcgtcctcg tggacgtcgg ctcgctcggg
     1321 ctcgaccccg acgaggcggt cgcgcgcctc cgcggcgagg gcgttctcct ctcgttcgcg
     1381 tcgaagcccg gcgtcctgcg ggcggtgacg cacctcgacg tctcggaggc ggacgtcgcc
     1441 gccgcgattc ccgcgatcgc gcgggcgctc tccgggcgcg aggcgcccgt cgccgtcggg
     1501 ctcgacgctc ccacgccgta ctgagaatcc gccgcgccgc cgcgaccgta tagcgcgacg
     1561 ggagcggaac cgtcacggcc cgatgcggcg cgggtgtcca gggtggacgc gccgcccccg
     1621 aagccatcgg acacgggagg aagccatgac gagaacacca cgcaggcgga tcgccgcgct
     1681 cgtcgccgcc gcggccgcgc tcgtgctgcc ggcggccgct caggcccagg cgccgccgcc
     1741 caccgtcgcc gacccgaacc tcgaggtgac gaccgtcgcg accgggctcg cccagccgac
     1801 cgggctcgcg ttcatcggcc cgaacgacat gctcgtgaac cagaaggcga acggccaggt
     1861 gcgccggatc ctgaacgggg agctcctggc cgacccggtg ctcgacctcg cggtcaactc
     1921 gcagtccgag cgggggctgc tcggcatcgc gacgcacccg aacttcttcg tgaacggctg
     1981 ggtctacctc ttctggacgg agtcgacgac cggcgcggac acgaatgtga acggcaacgt
     2041 cgcgctgctc ggcaaccgcg tcgaccggtt cgtctgggac ggctcgacgc tcacgttcga
     2101 tcggccgatc acgcgcttcc gggccgcgca gcccgcggtc gccgccgcgc cgccgctgcc
     2161 cgcgcagggc gcggcgggca accacaacgg cggcgtcgtc cgcttcggcc ccgacgggaa
     2221 gctctacgtg atcgtcggcg acaccggccg gcgcggctgg atgcagaacc tccgctgcgg
     2281 gccgttcggg ctcgagacca tctacacctg cccgcccgac acgcccgtcc ccgacgacgc
     2341 gttcggcggc ccgcagcccg acgacgcgca cttcaccggc gtgatcgtcc ggctgaacga
     2401 cgacggcacg acgcccgcgg acaacccgtt cttcgacgcg ggcgccgcga tcggcggcga
     2461 ggtcggcgcg aacatccaga agatctacgc gtacggcgtc agaaactcct acgggctcaa
     2521 cttcgacccg gtctcgggcg acctgtggca gagcgagaac ggcgacgaca cgttcagcga
     2581 gctcaaccgg gtcgagcccg ggatgaactc cggctggccg cagatcatgg gcccgctcga
     2641 ccggctcgcg gagtacaagg cgatcgagac gacgccgccc ttcgtcggcc tccagcagaa
     2701 ccgctggcct ccggcgaaca tcgcggacac gcctgaggag gctctcggcc gcctcgtcac
     2761 gttccccggc tcgcacttca gcgatccgga gttctcgtgg aagttcgagg tcgcgcccgc
     2821 cgggctcggc ttcgtctccg gccgggcgct cgggccgcag tacgacggcg acctgttctc
     2881 cggcggcgcc cggaacttcc tcgagggcgg ccacatcttc cggttcgacc tgaccggcaa
     2941 ccggcgcaag gtcggcgtgg acgatccacg catcgaggac cgcgtcgcgg acaacctcgc
     3001 gaagttcgag atcaccgaga gcgagagcct gctcttcggc acggacttcg ggctcacgac
     3061 cgacatcctg caaggcccgg acggggcgct ctacgtcctg tcgaacacgc gcagcgcgct
     3121 ctacaagatc tcgcggcgct aggagcgagg ggagagaggg ggcgcgggat ccggtgcccg
     3181 cgccccctcg ctcgactact cgtactggag cgcctcgagg acgttcagcc gcgccgcgcg
     3241 gcgcgcgggg gcgatcgccg cgaggatccc gacgatcacg gcgatgatcg cgaacgcgat
     3301 cagccagccc gtcggcacgg cgaactgcag gtcgaactgc gagagcgcct gcgtgacgag
     3361 gatcgcgagg aagatcccga gcggcacgcc gagggccgcg ccgatgaggg cggtgatgac
     3421 gctctcgtgc cggatcatcc ggcgagtctg acgcttcgtc atcccgaccg cgcgcagcat
     3481 cccgagctca cgcgtccgct cgtagaccgt gagcacgagc gtgttcacca tcccgaagag
     3541 gctgatgatg acggcgagcg ccagaaggac gtacagcatg atcaggaact ggttgaagtc
     3601 ctcgtcctgc tgcgtgatcc actgctcctt cgtctgcacc ttcgcgtcgg ggaagccggc
     3661 gacgcccgcc tcgagctgtt ccgtcgtcgc gtccgtcggg ttgccggcta cgttcgcgaa
     3721 gacgaactgg ttccgcggcc gctcgtagag cgagtcgaac gtctcgatcg agatcgagac
     3781 cgcgccgagg atcgggtaga agggcggcgg ctcgtagatc gccttcacct ccaggagcgc
     3841 ctcctcgccc gcgggggagg ccaccttcac gggcgacccg acctcgaggt cgttcgactc
     3901 ggcgaagtcc ttcgccacga tcgcgccgtc gcgccccagg tcggcgagca cgtcgtccga
     3961 gccgttctgc cacttgaacg tgtacgcctc cgcgatccgg tccgtctcga t
//
LOCUS       DASTFC010000317         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_189868,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000317 DASTFC010000000
VERSION     DASTFC010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189868"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFO88_09395"
     CDS             complement(<1..337)
                     /locus_tag="VFO88_09395"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HET9545782.1"
                     /translation="MSRERWLVRTVGESVLEELGRFGPQGALGDVVAAWPDAVGAGIA
                     EHAWPARIARDGTLTVAASSSVWAFELTQLEGTIRERLAAAVGDGAPRRLRFVPGRLP
                     ERGPKASEAT"
     gene            complement(334..1404)
                     /gene="recF"
                     /locus_tag="VFO88_09400"
     CDS             complement(334..1404)
                     /gene="recF"
                     /locus_tag="VFO88_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HET9545783.1"
                     /translation="MVVAHVRLRDFRSYPALELDLEPGLVLVTGPNGAGKTNLLEAVH
                     VGAQGFSPRTRAEAQLVRFDADAGRVALAGSEGGSGVETEVTIVRGEGKRLRLNGAAL
                     ASAEELRARLSALVFVPDRLAVVKGGPIVRRAYVDRMLGRLEPAQASLPGEYGRALAQ
                     RNEALRRVRAGLSSRDAVEPWTERVVELGASLDTARAGLVAALAPGFADRAAQLGLEE
                     AVLRYEEEPPTREALDERLDRDLRRGTTSIGPHLRDVAITAGPRELRSFGSQGEQRTA
                     VLALVLAEADLQAERRGSPPLLLLDDVLSELDAGRRRALAASLPPGAQTIVTATSRDA
                     FPADGPEPALVVEVRPGEARRV"
     gene            complement(1427..2533)
                     /gene="dnaN"
                     /locus_tag="VFO88_09405"
     CDS             complement(1427..2533)
                     /gene="dnaN"
                     /locus_tag="VFO88_09405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET9545784.1"
                     /translation="MKVTCRSEELAEKLAVAGRGVSTRTSVHVLTGILLRAEGGSLTL
                     AATDMELSLRLSLDATVDGDGATVVPGRLLVDIARLLPPGEVTLEHRAEEGTLKLTCG
                     PASYALNTFGVEDFPRLPELEGDESFSVERQAFLDTVAKVSRSASRDESRPVLTGILV
                     RFEAGRLVMAATDSYRLSVKETQLEGGPGRELEAIVPARALGELSRVAQAAASDTIEI
                     GIRDNQVVFEVDGVVLTARRIDGQFPNYKQLVPEAFEAEVELPRDEFLDVVRRTALMA
                     QRKSPLRLRFEEGQVTVSAQTQDVGEAQESLPVAYGGDTLEIGFNADFLRDGLESVSE
                     DKARLKLISPLRPGLIQGEGDDFLYLIMPIRLAG"
     gene            complement(2744..4135)
                     /gene="dnaA"
                     /locus_tag="VFO88_09410"
     CDS             complement(2744..4135)
                     /gene="dnaA"
                     /locus_tag="VFO88_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9545785.1"
                     /translation="MEQPLELTAETLWTEVAQRLRGALNEHTYTTWFGEASGEALDGD
                     VFVVAVPNDFTREWIENHFRGLVEAAVRDTIGDDRHVQVEVSAVEPAPRWEPDGAPPR
                     TWAAEGDGAAPDGGVRRERSGLNPKYTFDRFVIGSSNRFAHAAALAVAEAPAQAYNPL
                     FIYGGTGLGKTHLMQAIAAYLSEHTAGLTARYVTSETFMNDFINSLRDKRIEGFKHRY
                     RHYDVLLVDDIQFLEHKERIQEEFFHTFNSLYEAGRQIVISSDRPPRDIATLEARLRS
                     RFEWGLITDIQPPDLETRIAILRKKVHADGTHIPDPQLLTFIAGRISTNIRELEGALT
                     RVVAFSSLTGRSMTVDLAQEVLKDVFPQGELPEVSVERIQEAVIERFGISLQELTGDR
                     RAQSIVYPRQVAMYLCRELTDSSLPKIGKKFGGRDHTTVIHATSKIAKLIKEDRSVYN
                     LVQELTARIKQAR"
     gene            4503..4637
                     /gene="rpmH"
                     /locus_tag="VFO88_09415"
     CDS             4503..4637
                     /gene="rpmH"
                     /locus_tag="VFO88_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9545786.1"
                     /translation="MKRTYQPNVRRRKRRHGFRARMQTRAGRAILKRRRARGRKRLSA
                     "
BASE COUNT          668 a         1752 c         1684 g          695 t
ORIGIN      
        1 aggttgcctc ggacgccttc gggccgcgtt ccgggagcct cccggggacg aaccggaggc
       61 gcctcggagc cccgtcgccg acggcggcgg cgagacgctc gcggatcgtc ccctcgagct
      121 gcgtgagctc gaacgcccag acgctcgagc tcgcggccac cgtcagcgtc ccgtcgcgcg
      181 cgatgcgtgc gggccacgcg tgctcggcga tccccgcgcc gacggcgtcg ggccatgccg
      241 ccacgacgtc gccgagcgcg ccctgcgggc cgaagcggcc gagctcctcg aggacgctct
      301 cgccgaccgt gcgcacgagc caccgctcgc ggctcatacg cgccgcgcct ccccggggcg
      361 cacctcgacg acgagcgctg gctcgggccc gtccgcgggg aacgcatcgc ggctcgtcgc
      421 ggtgacgatc gtctgcgcac ctggcgggag cgatgccgcg agcgcccggc ggcgcccggc
      481 gtcgagctcc gagaggacgt cgtcgaggag gagcagcggg ggcgagccgc gccgctccgc
      541 ctgcaggtcc gcctccgcga ggacgagcgc gaggaccgcc gtccgctgct cgccctgcga
      601 cccgaagctc ctgagctcgc gcgggccggc ggtgatcgcg acgtcgcgga ggtgcgggcc
      661 gatagaggtc gtgccgcggc gcaggtcgcg gtcgagcctc tcgtcgagcg cctcccgggt
      721 cggcggctcc tcctcgtagc ggagcacggc ctcctcgagc ccgagctgcg cggcccggtc
      781 cgcaaacccc ggcgcgagcg ccgccacgag tccggcgcga gccgtgtcca gcgatgcgcc
      841 cagctcgacc acgcgctcgg tccacggctc gaccgcgtcg cggctcgaca gcccggcgcg
      901 cacgcggcgc agcgcctcgt tccgctgcgc gagcgcccgc ccgtactcgc cggggagcga
      961 ggcctgcgcc ggctcgaggc gcccgagcat ccggtcgacg taggcgcgcc ggacgatcgg
     1021 gccgcccttc acgaccgcga gccggtcggg gacgaagacg agcgcgctga gccgggcacg
     1081 gagctcctct gcgctcgcga gcgcggcacc gttcagccgc agccgcttgc cctcgccccg
     1141 gacgatcgtc acctccgtct cgacccccga cccgccctcg gagccggcga gcgccacccg
     1201 ccccgcgtcg gcatcgaagc ggacgagctg cgcctccgcg cgcgtgcgcg gcgagaaccc
     1261 ctgcgcgccg acgtgcaccg cctcgaggag gttcgtcttc ccggccccgt tcggcccggt
     1321 cacgagcacg aggcccggct ccaggtcgag ctcgagagcc gggtacgagc ggaagtcgcg
     1381 aagcctgacg tgcgccacca ccacgccggc accgccgctg cggcgctcag ccggccaggc
     1441 gaatcggcat gatcaggtag aggaagtcgt ctccctcccc ctggatcaac cccggccgga
     1501 gcgggctgat cagcttgagc cgggccttgt cctcgctcac ggactcgagc ccgtcccgca
     1561 ggaagtcggc gttgaagccg atctcgagcg tgtcgccgcc gtacgcgacc gggagcgact
     1621 cctgcgcctc gccgacgtcc tgcgtctgcg cgctcaccgt cacctggccc tcctcgaagc
     1681 ggaggcggag cggcgacttg cgctgcgcca tgagcgccgt gcggcgcacg acgtcgagga
     1741 actcgtcgcg gggcagctcc acctcggcct cgaacgcctc ggggacgagc tgcttgtagt
     1801 tggggaactg cccgtcgatg cggcgcgcgg tcaggacgac accgtccacc tcgaagacga
     1861 cctggttgtc gcggatcccg atctcgatcg tgtcgctcgc ggccgcctgc gcgacgcgcg
     1921 acagctcccc gagcgcgcgc gccggcacga tcgcctcgag ctcgcgcccc gggccgccct
     1981 cgagctgcgt ctccttcacg ctcagccggt acgagtctgt cgcggccatc acgagcctgc
     2041 ccgcctcgaa gcggacgagg atgcccgtca gcacgggccg cgactcgtcg cgcgatgcgg
     2101 agcggctgac cttcgccacc gtgtcgagga acgcctgccg ctcgaccgag aagctctcgt
     2161 cgccctcgag ctcgggaagc cgcgggaagt cctcgacgcc gaacgtgttc agcgcgtacg
     2221 acgccggccc gcacgtcagc ttgagcgttc cttcctccgc ccggtgctcg agcgtgacct
     2281 cgcccggcgg aagcaggcgc gcgatgtcca cgaggagacg tcccggcacg accgtcgcgc
     2341 cgtcgccgtc caccgtcgcg tcgagcgaca gccggagcga gagctccatg tccgtcgcgg
     2401 cgagcgtgag gctcccgcct tccgcacgca ggaggatccc cgtcaagacg tgaacgctcg
     2461 tccgcgtgga cacgccccgt ccggcgacgg cgagcttctc cgcgagctcc tcgctacgac
     2521 aggtgacctt catctgtgcg ccggcaacgg tatcggtact cattctttta agaacctcgt
     2581 tctagtagta aggagtgtgg atcacgggga agacggcgtg ccggcgaggg tccatgcggc
     2641 gcagcgctgg ggacggcggc tggggagaag gcgacgttcc tcacaagcct gcggccgcgg
     2701 gaggcttctc cacagaaacg gcccgcgctc gtccccatgg ttctcaacgg gcctgcttga
     2761 tgcgggccgt gagctcctgg acgaggttgt agacgctccg gtcctccttg atcagcttcg
     2821 cgatcttcga ggtcgcgtgg atgaccgtgg tgtggtcgcg cccgccgaac ttcttgccga
     2881 tcttcggcag cgaggagtcg gtcagctcgc ggcagaggta catcgcgacc tggcgcggat
     2941 agacgatcga ctgcgcgcgg cggtcgcccg tcagctcctg gagcgagatg ccgaagcgct
     3001 cgatcaccgc ctcctggatc cgctcgacgg agacctcggg gagctcgccc tgtgggaaga
     3061 cgtccttcag gacctcctgc gcgaggtcga ccgtcatcga gcggccggtg agggacgaga
     3121 aggcgaccac gcgcgtgagc gcgccctcga gctcgcggat gttcgtggag atccgtcccg
     3181 cgatgaacgt gaggagctgc ggatcgggga tgtgcgtccc gtccgcgtgc accttcttgc
     3241 ggaggatcgc gatgcgcgtc tcgaggtcgg gcggctggat gtccgtgatc agcccccact
     3301 cgaaccggga gcggaggcgc gcctcgagcg tcgcgatgtc gcgaggcggg cggtcggacg
     3361 agatgacgat ctgccggccc gcctcgtaga gcgagttgaa cgtgtggaag aactcctctt
     3421 ggatccgttc cttgtgctcg aggaactgga tgtcgtccac gagcagcacg tcgtagtggc
     3481 ggtagcggtg cttgaacccc tcgatccgct tgtcgcggag cgagttgatg aagtcgttca
     3541 tgaaggtctc gctcgtcacg tagcgcgcgg tgagcccggc cgtgtgctcg gagaggtagg
     3601 cggcgatcgc ctgcatgagg tgcgtcttgc cgagccccgt gccgccgtag atgaagagcg
     3661 ggttgtaggc ctgcgccggc gcctccgcga cggcgagcgc ggccgcgtgc gcgaagcggt
     3721 tcgaggagcc gatcacgaag cggtcgaagg tgtacttcgg gttgagcccg ctccgctcgc
     3781 gccggacgcc tccgtccgga gccgcgccgt ctccctcggc cgcccacgtg cgcggcggcg
     3841 cgccgtccgg ctcccagcga ggcgccggct cgacggcgga gacctcgacc tggacgtgcc
     3901 ggtcgtcgcc gatcgtgtca cggacggcgg cctcgacgag cccgcggaag tggttctcga
     3961 tccactcgcg cgtgaagtcg ttcgggacgg cgacgacgaa cacgtcgccg tcgagcgcct
     4021 cgccgctcgc ctcgccgaac cacgtcgtgt acgtgtgctc gttcagcgct ccccggagtc
     4081 gctgggccac ttcggtccac agggtctcag cagtcagctc caggggctgc tccacgcggt
     4141 gtttcggctc cttcccgacg ctcgaggagc ggcgatctta gcaacgtccc cctcgcccga
     4201 caaacgcgcg acctggggaa aagcggggag tcagaaaccg gtgttttgca ggcatttcgg
     4261 agttatccac agacccctcc acaatgtgga catctcgacc gaaccaccta gccatgcgcc
     4321 ctccctcctg tggatttccc ggtggacaaa tttctttccg cacagcgcgg aagacgtgtg
     4381 cgcgggtgcc cctcggaggg ccggcgcggc tgctctatac ttcgccgtcg gccgcccggc
     4441 ggccggcctc gagagacccg aacacgcgcg ctcgacgacg cgggcgccgg agagagacga
     4501 ccgtgaagcg aacctaccag ccgaacgtgc gccggcggaa gcgccgccat ggattcaggg
     4561 cgcggatgca gacccgcgcg ggccgcgcga tcctcaagcg ccggcgtgcc aggggccgca
     4621 agcgcctctc ggcctgaccc ggacctgtcc gtgaagcgcc gacaccgcct gtcccgctcc
     4681 cgggacttcg acgcggtcta ccgcaagggc cgctccgtct cgacgcgtta cctgaccgtc
     4741 tacagcttcc cgcgcgagga ggagctcgac ggcgacggcc cccggctcgg cctggccgt
//
LOCUS       DASTFC010000318         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_848934,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000318 DASTFC010000000
VERSION     DASTFC010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848934"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFO88_09420"
     CDS             <1..615
                     /locus_tag="VFO88_09420"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9545787.1"
                     /translation="GTDEEWDFAEAELREALDRRGLEYTPSEGEGSFYGPKIDLHMQD
                     SLGRSWQLGTVQLDLQMPKRFGLTYQGADNQLHTPAMIHRALLGSFERFLGVYLEHTG
                     GELPLFLAPEQVRVLPVKDDLAGDAAAVAEELRARGVRVGVDDRPETLGKRIRDAELE
                     KVPYVVVWGDRESRDAMAVRRRHGGQSTESLDALAGEIRDAASI"
     gene            760..1338
                     /gene="infC"
                     /locus_tag="VFO88_09425"
     CDS             760..1338
                     /gene="infC"
                     /locus_tag="VFO88_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET9545788.1"
                     /translation="MRPRRIVRPERPAEPQTRINEAIRAPRLRVVDEKGEQLGIKTRD
                     EALSLAADRDLDLVEVAANADPPVTRIMDYGKYRYEQEQKAKLARKHQTQINVKEIKF
                     RPKIGNHDYETKKGHVIRFLKDRAKVKVTIMFRGREATHPERGRDLLMRLADDVQDLG
                     VIESQPILDGRNMVMVLGPHRNVAQQTEASDA"
     gene            1331..1525
                     /gene="rpmI"
                     /locus_tag="VFO88_09430"
     CDS             1331..1525
                     /gene="rpmI"
                     /locus_tag="VFO88_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET9545789.1"
                     /translation="MPKQKTNKAAKKRFKVTGAGKLLRRHAMKSHNLEHKSAKRKRSF
                     ARDHEVSEADAPEVRKLLGR"
     gene            1549..1908
                     /gene="rplT"
                     /locus_tag="VFO88_09435"
     CDS             1549..1908
                     /gene="rplT"
                     /locus_tag="VFO88_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9545790.1"
                     /translation="MARVKRSVHARKKRRKVLEQAKGYFGQGHVSYRKAKEQILKADT
                     YAYRDRKTRKRVFRRLWITRINAAARREGLSYNQFVAGLKKAEIELDRKILADLAVND
                     PAAFSAVAARAKAALEA"
     gene            2001..2823
                     /locus_tag="VFO88_09440"
                     /pseudo
     CDS             2001..2823
                     /locus_tag="VFO88_09440"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
BASE COUNT          446 a          960 c         1057 g          360 t
ORIGIN      
        1 ggcacggacg aggagtggga cttcgccgag gccgagctcc gcgaggcgct cgaccggcgc
       61 gggctcgagt acacgccgag cgagggagag ggctcgttct acgggccgaa gatcgacctc
      121 cacatgcagg actcgctcgg ccgaagctgg cagctcggga ccgtgcagct cgacctgcag
      181 atgccgaagc ggttcgggct cacgtaccag ggcgcggaca accagctcca cacgccggcg
      241 atgatccacc gcgcgctcct cggctcgttc gagcgcttcc tcggtgtcta cctcgagcac
      301 acgggcggcg agctgccgct cttcctcgcg cccgagcagg ttcgcgtgct tccggtgaag
      361 gacgacctcg cgggcgacgc ggccgcggtc gccgaggagc tgcgcgcgcg cggcgtccgc
      421 gtcggggtcg acgaccggcc cgagacgctc ggcaagcgga tccgcgacgc ggagctcgag
      481 aaggtcccgt acgtggtcgt gtggggcgac cgggagtcgc gcgacgcgat ggcggtgcgc
      541 cgccggcacg gtgggcagtc gaccgagagc ctggacgccc ttgcagggga gatccgcgac
      601 gctgctagta tctgacctct gcaagcagga gccttcgctt ctcacctccc gagccccggg
      661 ctcccggagg ttcaaccgaa ttccaaggtt gatgcgaagc tgctgcgtgc agctttcgtc
      721 gtttgaagag gaggacacgc acagcttggt gacacgtcag tgaggcctcg ccgcatcgtc
      781 cggccggaac gcccggccga gccgcagacc cgcatcaacg aggcgatccg cgcgccgcgc
      841 cttcgcgtgg tggacgagaa gggggagcag ctcgggatca agacgcggga cgaggctctc
      901 tcgctcgcgg ccgatcgcga tctcgacctc gtcgaggtcg ccgcgaacgc cgatccgccc
      961 gtgacgcgga tcatggacta cggcaagtac cggtacgagc aggagcagaa ggccaagctc
     1021 gcccggaagc accagacgca gatcaacgtc aaggagatca agttccgccc gaagatcggc
     1081 aaccacgact acgagacgaa gaaggggcac gtgatccggt tcctgaagga ccgcgcgaag
     1141 gtgaaggtga cgatcatgtt ccggggccgc gaggccacgc atccggagcg cggccgcgac
     1201 ctgctcatgc gcctcgcgga cgacgtccag gacctcggcg tgatcgagtc gcagccgatc
     1261 ctcgacggcc ggaacatggt catggtgctc ggcccgcacc gcaacgtggc ccagcagacg
     1321 gaggcgagcg atgcctaagc aaaaaaccaa caaggccgcg aagaagcggt tcaaggtgac
     1381 gggcgccggc aagctcctgc gccgtcacgc catgaagagc cacaacctcg agcacaagtc
     1441 ggccaagcgc aagcggtcgt tcgcccgcga ccacgaggtc tccgaggcgg acgcgcccga
     1501 ggtgcgcaag ctgctcggcc gctagcccgc gcggaaggag agacgaagat ggcacgcgtc
     1561 aagcgatcgg tgcacgcccg caagaagcgc cggaaggtgc tcgagcaggc gaagggctac
     1621 ttcggccagg ggcacgtctc ctaccgcaag gcgaaggagc agatcctcaa ggcggacacg
     1681 tacgcgtacc gcgaccgcaa gacgcgcaag cgggtgttcc ggcggctctg gatcacgcgg
     1741 atcaacgcgg ccgcgcggcg cgaggggctc tcctacaacc agttcgtcgc gggcctgaag
     1801 aaggcggaga tcgagctcga ccggaagatc ctcgccgatc tcgcggtgaa cgaccccgcc
     1861 gcgttctcgg cggtcgccgc gcgggccaag gccgcgctcg aggcctaacg gccgggacgc
     1921 gggacgacgc gccggtcgcg gcgcagtttc gccggcgcgc ggacgacttc gagcgcggcg
     1981 ggtcggcgct ctacgcgcgg ctcgcgcggg agatcgccgg cgacccggtc gtggaagaga
     2041 tctgcggggc gcgcggctcg tcgtgggacg cgcccctcgc gctcttcggc ggcgtccacc
     2101 tgctcgcgct cggcggggag gagccggatc cgtggtcgcg gctcccggag gtgttgcggg
     2161 tacaccgcgc ccggctcgcg cgcttcgtcg cggaggagcc ggtgcagacg aacgaggtgc
     2221 agcgcgcctg ggggctcctc ccggggttcc tctcggccgg cgcgccggtc ctcgacctcg
     2281 tggagctcgg cccgagcgcg ggcctgaacc tcctttggga ccggtggggc taccggtacc
     2341 cggccgggtc gtggggcgcg gacgacgcgc cgctcgtgct ccacggggag gccgagggcg
     2401 ggccgccggc ggagctgctc gagctgcgcc cgctcgtgcg ggggcggctc gggatcgacc
     2461 gcgcccccgt cgacgtgcgc gacgacgcgg cggccctgcg cctgcaggcg ttcgtgtggg
     2521 ccgatcagga ggagcggctc gcgcggctgc gggcggcgct cgaggtcgcg cggcgggaac
     2581 cgccggagct cgtcgccggc gactacctcg aggagctgcc gcgcgtgctc gcggcgcggg
     2641 cgccggacgc gctcacggtc gtctttcatt ctgcgtcgac cgcgtacctc tcgccggaaa
     2701 accgcgcgcg gctcgccggg atcctcgagg aggccggggc ggaggcgccg ctcgcgtggc
     2761 tctcgtacga gcacgcgggc gacgacgtcg acgggacgat cgggttcgag acgttcgcgc
     2821 tcg
//
LOCUS       DASTFC010000319         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1178532,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000319 DASTFC010000000
VERSION     DASTFC010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178532"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="VFO88_09445"
     CDS             <1..2035
                     /locus_tag="VFO88_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9545791.1"
                     /translation="HVLSSSLDHEETLARLGRLSVPRLADSYVVMLVDGGRLVPAGIA
                     HSEPEGLDALEELVRRYPPALDEPHGAGRVARTGEPELVTAVTDETLTADARDDEHLA
                     LLRRVGARSSVVVPLRVADRTLGALALSVGPSGRAFGADDLRLAEDVGRRAATAIENS
                     LLHRAEHEARARAELLQGVTERLAGARTQREVADVLVREGVSALGAAAGWVALLGPDG
                     RELELMAAVGYPPDVVEQYRSIPVESAIGAQDILVHGRQAWLESAEEVVARFPQHAPA
                     YVRMGYEALAVVPLEVGGVRTGLVALNFPERTSFAQGERAVLLAVVGQCSQALERARL
                     LDALAERANAAVVLAHVDDGVFQVAADGTVRYWNPAAEHLTGIAAGAAVGRQVGELLG
                     GWEEASAAIEVGTRPGAATSGEAVPLVVAGEERWLAVSGVRTPDGTVYAFRDLTSERE
                     LERARRDFLSTASHELRTPLSAVYGASLTLVHRDLDEEQRDAMLDMIVRECDRLTRIV
                     EDLLLANGLDLDHVHVALASADAVALAHEVVSVRGSTLPEGLTLAVEPNGGAIEVACD
                     PERLRQVLLNLVDNAVKYSPDGGRIRVAVAADDRVARFVVTDEGIGIPAGEQRRIFEK
                     FYRLDPELRRGVGGSGLGLYISRELVRRMDGRIEVESEPGRGSRFVVELPLAR"
     gene            2054..2488
                     /locus_tag="VFO88_09450"
     CDS             2054..2488
                     /locus_tag="VFO88_09450"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HET9545792.1"
                     /translation="MAERVPIPGSEQGATIRNIFVVAILSIVTLGIYVFFWWYFVNRE
                     MADLGRARGTNELGTSPGTSLLAITLGALVIVPAIMSLIGTFRRVQATQRLTASSEPI
                     NGWIALVLLLVVPPAFYAYLQSGLNPSWETLRAEAAAPALEQ"
     gene            2525..>2883
                     /locus_tag="VFO88_09455"
     CDS             2525..>2883
                     /locus_tag="VFO88_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545793.1"
                     /translation="MQGVDLRPLGVGEILDVGIKITWRHAWTLIRTVLLVVAPVQVLA
                     AVVQLSALPEGAFSPVPAPGEPDPLAEAEDAEFAAWFAAILVGALLGFVATTLATGAC
                     YKAVADAYLGETPNWRTS"
BASE COUNT          337 a         1082 c         1064 g          400 t
ORIGIN      
        1 ccacgtcctc tcgagctcgc tcgaccacga ggagacgctc gcgcgcctcg gccgcctctc
       61 cgtgccgcgc ctcgccgaca gctacgtcgt catgctcgtc gacggcgggc ggctcgtccc
      121 cgcggggatc gcgcattcgg agcccgaggg gctcgacgcc ctggaggagc tcgtgcggcg
      181 ctacccgccc gcgctcgacg agccgcacgg cgccggacgg gtcgcccgca cgggcgagcc
      241 ggagctcgtg accgccgtca ccgacgagac gctcaccgcg gacgcgcggg acgacgaaca
      301 cctcgctctc ctccgccgcg tcggcgcgag gtcgtcggtc gtcgtgccgc tccgcgtcgc
      361 cgaccggacg ctcggcgcgc tggcgctctc ggtgggcccg tcggggaggg cgttcggggc
      421 ggacgacctt cgtctcgccg aggacgtcgg ccgccgcgcc gcgacggcga tcgagaactc
      481 gctcctccac cgggccgagc acgaggcgcg cgcccgcgcg gagctcctcc agggcgtcac
      541 cgagcgcctt gcgggcgccc ggacgcagcg cgaggtcgcc gacgtcctcg tccgggaggg
      601 cgtgtcggcg ctcggcgccg cggccggctg ggtcgcgctc ctcggccccg acgggcgcga
      661 gctcgagctg atggccgcgg tcggctaccc gcccgacgtc gtcgagcagt accggagcat
      721 ccccgtcgag agcgctatcg gcgcccagga catcctcgtg cacggccgcc aggcctggct
      781 cgagtcggcc gaggaggtcg tcgcgcggtt cccgcagcac gcgcccgcgt acgtgcggat
      841 gggctacgag gcgctcgcgg tcgtcccgct cgaggtcggc ggcgtccgca ccggcctcgt
      901 cgcgctcaac ttccccgagc ggacgagctt cgcgcagggc gagcgcgcag tgctgctcgc
      961 cgtggtgggc cagtgctcgc aggcgctcga gcgcgcgcgg ctcctcgacg cgctcgcgga
     1021 gcgcgcgaac gccgccgtcg tgctcgccca cgtggacgac ggcgtcttcc aggtcgcggc
     1081 ggacggcacc gtgcgctact ggaacccggc ggccgagcat ctgaccggga tcgccgcggg
     1141 cgccgcggtg ggccggcagg tcggggagct gctcggaggc tgggaggagg cgtccgcggc
     1201 gatcgaggtg ggcacgcgcc cgggcgccgc cacctcgggc gaggcggttc cgctcgtggt
     1261 cgccggcgag gagcgctggc tcgccgtctc gggcgtgcgc acgccggacg ggacggtcta
     1321 cgccttccgc gacctcacct ccgagcggga gctcgagcgg gcgcggcgcg acttcctctc
     1381 caccgcctcc cacgagctca ggactccgct cagcgcggtc tacggcgcct cgctcacgct
     1441 cgtgcaccgc gacctcgacg aggagcagcg cgacgcgatg ctcgacatga tcgtccgcga
     1501 gtgcgatcga ctcacccgga tcgtcgagga tctcctgctc gcgaacggcc tcgatctcga
     1561 ccacgtgcac gtggcgctcg cgagcgcgga cgcggtcgcc ctagcgcacg aggtcgtctc
     1621 cgtccgcggc tcgacgctcc ccgaggggct cacgctcgcc gtcgagccga acggcggcgc
     1681 gatcgaggtc gcctgcgatc ccgagcggct ccggcaggtg ctcctgaacc tcgtggacaa
     1741 cgccgtcaag tactcgccgg acggaggccg catacgcgtc gcggtggcgg cggacgaccg
     1801 ggtcgcgcgc ttcgtcgtca ccgacgaggg gatcgggatc ccggcggggg agcagcggcg
     1861 gatcttcgag aagttctacc gcctggatcc ggagctccgg cgcggcgtcg gcgggagcgg
     1921 gctcgggctc tacatcagcc gcgagctcgt ccggcggatg gacggccgga tcgaggtcga
     1981 gtcggagccc ggccgcgggt cgcgcttcgt cgtcgagctc ccgctcgcgc ggtagcgtgg
     2041 tacggtcggg cgcgtggcgg agcgggtgcc gatccccggc agcgagcagg gcgcgacgat
     2101 ccggaacatc ttcgtcgtcg cgatcctctc gatcgtcacg ctcgggatct acgtcttctt
     2161 ctggtggtac ttcgtgaacc gggagatggc cgacctcggg cgggcgcgcg ggacgaacga
     2221 gctcgggacg agcccgggga cgtcgctcct cgcgatcacg ctcggcgcgc tcgtgatcgt
     2281 ccccgcgatc atgtcgctca tcgggacgtt caggcgggtg caggcgacgc agcgcctcac
     2341 ggcgagctcg gagccgatca acggctggat cgcgctcgtg ctcctgctcg tcgtcccgcc
     2401 cgcgttctac gcctacctcc agagcggcct caacccctcg tgggagacgc ttcgcgcgga
     2461 ggcggcggcg cccgccctcg agcaatagcc gacccgtccc gggcgcgcgt tatcgtcagc
     2521 gaccatgcag ggcgtcgacc ttcggcccct cggggtcggc gagatcctcg acgtcggcat
     2581 caagatcacc tggcggcacg cgtggacgct catccgcacc gtcctcctgg tcgtggcgcc
     2641 ggtgcaggtc ctcgcggcgg tcgtccagct ctccgcgctc ccggagggcg ccttctcgcc
     2701 cgtccccgcg ccgggcgagc cggacccgct cgccgaggcg gaggacgccg agttcgcggc
     2761 ctggttcgcc gcgatcctcg tcggtgcgct gctcggcttc gtcgcgacca cgctcgccac
     2821 cggcgcctgc tacaaggcgg tcgccgacgc ctacctcggc gagacgccga actggaggac
     2881 gtc
//
LOCUS       DASTFC010000320         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1112660,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000320 DASTFC010000000
VERSION     DASTFC010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112660"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /gene="thrC"
                     /locus_tag="VFO88_09460"
     CDS             complement(<1..683)
                     /gene="thrC"
                     /locus_tag="VFO88_09460"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9545794.1"
                     /translation="MTVPLVERFRERLPVSSATPVVTLGEGSTPLVRAPRLSERLGAE
                     VWLKWEGANPTGSFKDRGMTVAVAKALEDGAAAVVCASTGNTSASAAAYAARAGLPAL
                     VLLPAGAVALGKVAQARAAGAVLLQVEASFDEALAAARELDARGTHALVNSLNPYRLE
                     GQKTAAFELAEELGGPPDVLALPYGGGGNTAAYARGFREAGEGLPRFLLGEAADRGGT
                     AASAIRIGTP"
     gene            complement(680..1981)
                     /locus_tag="VFO88_09465"
     CDS             complement(680..1981)
                     /locus_tag="VFO88_09465"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET9545795.1"
                     /translation="MSTPVDVPVALLGYGTVGSAVERLLRESADDIERATGHRLRVVR
                     ALVRDPAKERPAPAGPGLLTDDFAAIRDDPDVAVVAEVMGGVEPTGEYVLELLRAGKP
                     VVSANKQLVARRGAELFAAASAAGVQLRFEASVCAAIPVVKVLREALVATHVHRILGI
                     VNGTTNFVLTEMEAGRSYDEALAEAQRRGFAEADPTEDVNGADAAAKMAILATVAFGS
                     RVELGDVVTTGIEGLSEAHVRAARELDAVVRLVGAAELADGRVDVRVRPALIGRHHPL
                     AAVEGAFNAVMLNGDAIREIVLEGPGAGGIETASAVVSDLVSVVGTAGTGFLENDACW
                     RELRRLPPEEFRAPFYVQLVVDDRPGVLADVAAGLAAHGVSVARLIQEQGDGDATLHL
                     VTHETAEGALDAALGEIARTPGTRRPPSVLLVVPDRDRPGT"
     gene            complement(1990..>2930)
                     /locus_tag="VFO88_09470"
     CDS             complement(1990..>2930)
                     /locus_tag="VFO88_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9545796.1"
                     /translation="RLYVVEQAGRILVLDGGGPAGEPFLDIRGDVAAGGERGLLSVAF
                     HPDYAENGRLYVNYTNSDGDTRVVEYRANETRAAVAPGTARELLAVDQPYANHNGGQL
                     AFGPDGLLYVGMGDGGSGGDPESRAQDLGDRLGKLLRLDVDAEGADWEVAAYGLRNPW
                     RFSFDRETGDLHLADVGQDAFEEVNALPWPQDGLVNFGWPTFEGTSRFDDRPLTEGGR
                     LVDPIRTYGRDAGCSVTGGFAYRGEAMPGLAGRAFYGDYCSGTIWSYRFVDGAARGAR
                     REELRVPLLTSFGEDTAGELYLVSHQGTVFRLVEAR"
BASE COUNT          363 a         1163 c         1063 g          341 t
ORIGIN      
        1 ggcgtcccga tgcggatcgc gctcgccgcc gtcccgccgc ggtcggccgc ctcgccgagc
       61 aggaagcgcg gcagcccctc ccccgcctcg cggaacccgc gcgcgtaggc ggccgtgttg
      121 ccgccgccgc cgtacgggag cgcgagcacg tcgggcggcc cgccgagctc ctcggcgagc
      181 tcgaacgcgg ccgtcttctg cccctcgagc cggtacgggt tgagcgagtt gacgagcgcg
      241 tgcgtccccc gcgcgtcgag ctcgcgtgcc gccgcgagcg cctcgtcgaa gctcgcctcg
      301 acctggagca gcaccgcgcc cgccgcgcgg gcctgggcga ccttgcccag cgcgaccgcg
      361 ccggccggga gcaggacgag cgccggcagc cccgcacgcg ccgcgtacgc cgccgccgag
      421 gcggacgtgt tcccggtcga cgcgcagacg accgcggcgg cgccgtcctc gagcgccttc
      481 gcgacggcaa ccgtcatccc gcggtccttg aacgagcccg tcgggttcgc accctcccac
      541 ttgagccaga cctccgcgcc gagccgctcc gacaggcgcg gcgcgcggac gagcggggtc
      601 gagccctccc ccagcgtgac gaccggcgtc gcggacgaga ccggcagccg ctcgcggaac
      661 cgctcgacga gcgggacggt cacgtccccg gacggtcgcg gtcgggcacg acgagcagga
      721 cggacggcgg ccgccgcgtc ccgggcgtcc gcgcgatctc gccgagcgcg gcgtcgagcg
      781 cgccctcggc cgtctcgtgc gtgacgaggt ggagcgtcgc gtcgccgtcg ccctgctcct
      841 ggatcaggcg cgcgaccgac acgccgtgcg ccgcgagccc cgccgcgacg tcggcgagca
      901 cgccgggccg gtcgtcgacg acgagctgga cgtagaacgg ggcgcggaac tcctccggcg
      961 gcagccgccg gagctcgcgc cagcacgcgt cgttctcgag gaagccggtg cccgccgtgc
     1021 cgacgacgct cacgaggtcg gagacgaccg ccgacgccgt ctcgatcccg cccgcgcccg
     1081 gcccctcgag cacgatctcg cggatcgcgt cgccgttcag catgaccgcg ttgaacgcgc
     1141 cctcgaccgc ggcgagcgga tggtggcgcc cgatgagcgc cggccgcacg cgaacgtcga
     1201 cacgcccgtc cgcgagctcg gccgcgccga cgagccggac gaccgcgtcg agctcccgcg
     1261 ccgcgcgcac gtgcgcctcc gagaggccct cgatcccggt cgtcacgacg tcgccgagct
     1321 ccacgcgcga cccgaacgcg accgtggcga ggatcgccat cttcgcggcg gcgtcggcgc
     1381 cgttcacgtc ctcggtcggg tcggcctccg cgaagccgcg ccgctgcgcc tcggcgagcg
     1441 cctcgtcgta gctgcgccca gcctccatct ccgtgaggac gaagttcgtc gtcccgttca
     1501 cgatcccgag gatccggtgg acgtgcgtcg cgacgagcgc ctcgcggagc accttcacga
     1561 ccgggatcgc cgcgcagacg ctcgcctcga accggagctg gacgcccgcg gcggacgcgg
     1621 ccgcgaagag ctccgccccg cggcgcgcga cgagctgctt gttcgccgag acgaccggct
     1681 ttcccgcgcg aagcagctcg agcacgtact cgcccgtcgg ctcgaccccg cccatcacct
     1741 ccgcgacgac ggccacgtcg gggtcgtcgc ggatcgccgc gaagtcgtcc gtcaggaggc
     1801 cgggcccggc gggcgcgggc cgctccttcg ccgggtcgcg gacgagcgcg cgcacgacgc
     1861 ggagccggtg gccggtcgcc cgctcgatgt cgtccgcgct ctcccgcagg agccgttcca
     1921 ccgcggagcc gaccgtgccg tagccgagca gggcgaccgg gacgtcgacg ggcgtgctca
     1981 cgggcgccat catcgcgcct cgaccagccg gaacaccgtt ccctggtgcg agacgaggta
     2041 gagctcgccc gccgtgtcct cgccgaacga ggtgaggagc ggcacccgga gctcctcccg
     2101 gcgggcgccg cgcgccgcgc cgtcgacgaa gcgatagctc cagatcgtcc ccgagcagta
     2161 gtcgccgtag aacgcccgcc cggcgagacc gggcatcgcc tcgccgcggt acgcgaagcc
     2221 gccggtgacc gagcagcccg cgtcgcgccc gtacgtccgg atcgggtcga cgagccgccc
     2281 gccctcggtg agcggccggt cgtcgaagcg gctcgtgccc tcgaacgtcg gccagccgaa
     2341 gttcacgagc ccgtcctgcg gccacgggag cgcgttcacc tcctcgaacg cgtcctggcc
     2401 gacgtcggcg aggtggagat cgccggtctc gcggtcgaac gagaaccgcc acgggttccg
     2461 gagcccgtac gcggcgacct cccagtcggc gccctcggcg tcgacgtcca ggcgcaggag
     2521 ctttccgagc cggtcgccga gatcctgcgc gcggctctcc gggtcgccgc cggagccgcc
     2581 gtcgcccatc ccgacgtaga gcagcccgtc gggcccgaac gcgagctggc ccccgttgtg
     2641 gttcgcgtac ggctggtcga ccgcgaggag ctcgcgcgcg gtgccgggcg cgacggcggc
     2701 gcgcgtctcg ttcgcgcggt actcgacgac ccgggtgtcg ccgtcggagt tcgtgtagtt
     2761 cacgtagagg cgcccgttct cggcgtagtc cgggtggaac gcgaccgaga ggaggccgcg
     2821 ctcgccgccg gccgcgacgt cgccgcggat gtcgaggaac ggctcgcccg ccgggccgcc
     2881 gccgtccagg acgaggatcc gccccgcctg ctcgacgacg tagagccgcc
//
LOCUS       DASTFC010000321         5870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_289071,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000321 DASTFC010000000
VERSION     DASTFC010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289071"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFO88_09475"
     CDS             <1..816
                     /locus_tag="VFO88_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545797.1"
                     /translation="ADELGGQMRWVRRLPGLGEWGRIVDWRHGQLDLLRNVEFVPRTR
                     LDAAAVRDYGAQIVIVATGARWATDGLNAATHDPIPGANASLPHVLTPEQLMLDGKRP
                     PGERVLVWDCEGYFAGTGVAEKLALDGHSVDLATSLHVIAPYTDETLEGPQVRQRLHE
                     VGVRQHRGVQLLEVTPGGVRATDEFGEPLELETDAVVLVTQRLSADALYHELVGDPDA
                     LAAEGIEAVYRIGDCVSPRIIAEAVFDGHRLGREIDTEHPDRPLPYVRERLVL"
     gene            complement(869..1804)
                     /locus_tag="VFO88_09480"
     CDS             complement(869..1804)
                     /locus_tag="VFO88_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9545798.1"
                     /translation="MQRRTKLVALLAVAGTLAAAAGAALALSGAAAAQQENEAVHACR
                     HPNGGWVRLVQSPENCRPREQAISWNVAGPPGPEGPAGPKGDKGDPGAGLTKLEDLAG
                     VACTPDGGGSGKVELDFAGDDTVLFRCVPGGGDPDPDPDPPPPPPDRPDLVLNEVDYD
                     QVGADGDGFVELKNTGTTPIDLAGIALVFVDGADGEEYRRFALTGTLEPGGYHVVAAD
                     PQNGAPDGLALVDTESGDLIDALSYEGAITAAVIGTRTYSLVEGTMLPANVADSNTVD
                     GSLSRIPDGRDTDDAASDWQFTTTLTRGAANVAAP"
     gene            2050..2982
                     /locus_tag="VFO88_09485"
     CDS             2050..2982
                     /locus_tag="VFO88_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545799.1"
                     /translation="MRRVIPLLACLALLVPATAQAQGEPLATLDGGVSGQITHWLTFG
                     GGVTGEPVVHLEEPGLYRFMILDNSAGHNFWLTGPGPNQLVFRTVFESMELPPPDPNG
                     PPQQFDRVLEPGIYRYQCENHAFMTGWFSVGDVLVVQTETGHGIITSDPPGANWPTNR
                     GAHFPEGTEVTVTATPQNSSFTFNGWMMGCSGLGPCVVTISGRTEVRALFGTPVAATA
                     PASISRVAVVKRNLRRTVRVTLSVKRTADVTTQLRKRTKVVASAKARLTAGSRLVSLA
                     VPRRAKGGLYTVRVAMRDLEGRTFSASRSIRVPR"
     gene            complement(2993..3436)
                     /locus_tag="VFO88_09490"
     CDS             complement(2993..3436)
                     /locus_tag="VFO88_09490"
                     /inference="COORDINATES: protein motif:HMM:NF044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET9545800.1"
                     /translation="MDIVLRAAVAFLFITLLLRVIGRRELSSLEPFDVILLVVIGDLM
                     QQGVTQSDMSMTGAVLAVGTFAGLAVLSSYIVYRFRAARPVLEAAPLVLLQDGQPIEH
                     NLRHERMTLEELAAEARQQQITSLDDVRWAVLEGNGKVSFIPKSG"
     gene            complement(3440..3961)
                     /locus_tag="VFO88_09495"
     CDS             complement(3440..3961)
                     /locus_tag="VFO88_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545801.1"
                     /translation="MAGAPQFLVLEKSRDSQHPVELWVRRAVLAGIALLALLALLNVF
                     GQRPVTTAADGAQARIEVQAPTALRGGVLFMGRITIETQEELENATLVLDRGWLESMH
                     VNTIEPAPTEEESRDGRLALSYGRLAAGETAVVYLQLQVNPTNVGRRSQSVELHDGEE
                     LVARTDRTVTILP"
     gene            complement(4003..5304)
                     /locus_tag="VFO88_09500"
     CDS             complement(4003..5304)
                     /locus_tag="VFO88_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9545802.1"
                     /translation="MSDGSLPAPGASPWRLGLARLGAFALSETGRVRLALGLVALHVA
                     DDNYLRPEPGTSPGDHLASGLVPIAVLGAVAYLYPRLRAGARAATAMTFGALAIAIGV
                     PGAYYLADGTASGDHYTGLLALVAGVVLLASGPVTLWQARRRGGTRRRRYALRALTVA
                     AGTALAFVLVWLVVFPVSLAYVYTHTGRTSSQPAPGAPFEPVTVTTSDSLDLAAWHIP
                     SENGAAVVVFPGATRADEARMLADNGYGVLLLESRGQGGSEGDIVRWAGDRDLLAGAA
                     FLAGQPGVDPARIGAIGYSIGGEILLETAAKSDAFAAIVSDGAGERVGEQDTEGAGRI
                     LAGPLQTVLTAAVTVFSNHGPPPPIVERIGLIAPRPVFLISAEHGIGNENGRQPRYYA
                     AAGEPKELWEVPGAGHTGGLEAQPAEYERRVVEFFDGSLLP"
     gene            complement(5301..>5870)
                     /locus_tag="VFO88_09505"
     CDS             complement(5301..>5870)
                     /locus_tag="VFO88_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545803.1"
                     /translation="LLFHPPGGDPVYEGVRGDVDAWLFVHIGMLLALPFLGIAAYLLL
                     DGLHGLAALVSRVALVFFLVFYTAYEATVGVATGVLVDHANGLAGAEQAAVGDAIQEL
                     NRNAVLGDPISVALALGSLGWIVAMVAAAVALRRAGAGWTITVLVGLAAAFAVHPPPI
                     GPIALVCFVAAAVLLERSRARATGAQGAS"
BASE COUNT          785 a         2130 c         2198 g          757 t
ORIGIN      
        1 gcggacgagc tcggcggcca gatgcgctgg gtgcgccggc tccccgggct cggcgagtgg
       61 gggcggatcg tcgactggcg ccacgggcag ctcgacctgc tccggaacgt cgagttcgtc
      121 ccgcgcacgc ggctcgacgc ggcggccgtg cgcgactacg gcgcgcagat cgtgatcgtc
      181 gcgaccggcg cgcgctgggc gacggacggc ctcaacgccg ccacgcacga cccgatcccc
      241 ggcgcgaacg cgtcgctccc gcacgtcctc acgcccgagc agctcatgct ggacgggaag
      301 cgcccgccgg gcgagcgggt gctcgtctgg gactgcgagg gctacttcgc cggcacgggc
      361 gtcgcggaga agctcgcgct cgacgggcac tccgtcgacc tggcgacgag cctccacgtg
      421 atcgcgccct acacggacga gacgctcgag ggcccgcagg tgcggcagcg cctgcacgag
      481 gtcggcgtcc ggcagcaccg gggcgtccag ctcctcgagg tgacgcccgg aggcgtgcgc
      541 gcgacggacg agttcggcga gccgctcgag ctcgagacgg acgccgtcgt cctcgtgacg
      601 cagcggctct cggcggacgc gctctaccac gagctcgtcg gcgaccccga cgcgctcgcc
      661 gccgagggga tcgaggccgt ctaccggatc ggcgactgcg tctcgccgcg gatcatcgcc
      721 gaggcggtct tcgacgggca ccggctgggg cgcgagatcg acacggagca tccggaccgc
      781 ccgctcccgt acgtccgcga gcggctcgtg ctgtaggcgc tgaagcgagg cggcgggcgc
      841 tcgagccgcc cgccgcctcg ccccgggctc agggagccgc gacgttcgcg gcgccccggg
      901 tgagcgtcgt cgtgaactgc cagtccgacg cggcgtcgtc ggtgtcacgg ccgtccggga
      961 tccggctcag cgatccgtcg accgtgttcg agtccgcgac gtttgccggg agcatcgtcc
     1021 cttcgacgag gctgtacgtt cgcgtcccga tcaccgccgc cgtgatcgcg ccctcgtagg
     1081 agagcgcgtc gatcaggtcg ccgctctccg tgtcgacgag cgcgagcccg tccggcgcgc
     1141 cgttctgcgg gtcggccgcg acgacgtggt agccgcccgg ctcgagcgtc cccgtgagcg
     1201 cgaagcgccg gtactcctcg ccgtcggcgc cgtcgacgaa gacgagcgcg atcccggcca
     1261 ggtcgatcgg ggtcgtgccc gtgttcttca gctcgacgaa cccgtcgccg tccgcgccga
     1321 cctggtcgta gtcgacctcg ttcaggacga gatccggcct gtcgggcggc gggggcgggg
     1381 ggtcggggtc gggatccggg tcgccgccgc cggggacgca gcggaagagc acggtgtcgt
     1441 cgcccgcgaa gtcgagctcg acctttccgc ttccgccgcc gtccggcgtg caggcgacgc
     1501 cggcgaggtc ctcgagcttc gtgaggcccg cgccgggatc gcccttgtcg cccttcggcc
     1561 ccgcgggccc ctccggcccg ggcggtcccg cgacgttcca cgagatcgcc tgctcgcgcg
     1621 ggcggcagtt ctccggcgac tggaccaggc gcacccagcc gccgttcggg tggcggcagg
     1681 cgtgcaccgc ctcgttctcc tgctgcgcgg ctgccgcgcc gctcagcgcg agcgccgccc
     1741 cggccgccgc cgccagcgtg cccgcgacgg cgagcagggc gacgagcttc gtgcgtctct
     1801 gcatgtggct ccttccggtt ggatgacccc gaccggaata ccgccgggga ggcggcctgc
     1861 accagtcgtc cgcttcggcc gggtgcgtcg caaagccgca tgcacacgga cgcctgtgga
     1921 ggaaccggca cagactcggc acgccgctcc ggccgccacg gcctcggccg gctcacccgc
     1981 gcatgcacat caacgggccg cggcgaggga gacttggcag aagtactgcg aaggcgtatt
     2041 atcgcgcgcg tgcggcgggt catccccctt ctcgcctgtc tcgcgcttct cgtccctgcc
     2101 accgcgcagg cccagggaga gccgctcgcc acgctcgacg gcggcgtctc cggccagatc
     2161 acgcactggc tcacgttcgg cggcggcgtg accggcgagc ccgtcgtcca cctcgaggag
     2221 ccgggcctct accggttcat gatcctggac aactcggcgg gtcacaactt ctggctcacg
     2281 gggccgggcc cgaaccagct cgtcttccgg acggtcttcg agagcatgga gctgccgccg
     2341 cccgacccga acgggccgcc gcagcagttc gaccgcgtgc tcgagccggg gatctaccgc
     2401 taccagtgcg agaaccacgc gttcatgacc ggctggttct ccgtcggcga cgtgctcgtc
     2461 gtgcaaacgg agaccggcca cgggatcatc acgagcgacc cgccgggggc gaactggccg
     2521 acgaaccgcg gcgcgcactt cccggagggg accgaggtca ccgtgacggc gacgccgcag
     2581 aactcgtcgt tcaccttcaa cggctggatg atgggctgca gcggcctcgg gccgtgcgtc
     2641 gtcacgatca gcggccgcac cgaggtgcgc gcgctcttcg ggacgccggt tgcggcgacc
     2701 gcgccggcgt ccatctcgag ggtcgccgtg gtgaagcgga acctccgccg cacggttcgc
     2761 gtgaccctga gcgtcaagcg cacggccgac gtgacgacgc agctccgcaa gcggacgaag
     2821 gtggtcgcct cggcgaaggc gcgcctgacg gcggggtcgc ggctcgtgtc gctcgcggtg
     2881 ccgcggcgcg cgaagggtgg gctctacacc gtgcgcgtcg ccatgcggga tctcgagggc
     2941 cgcacgttca gcgcgagccg ctcgatccgt gtgccgcgct gagcccgcgg cgctagccgc
     3001 tcttcgggat gaagctcacc ttgccgttgc cctcgagcac ggcccagcgg acgtcgtcga
     3061 gcgaggtgat ctgctgctgg cgcgcctcgg cggcgagctc ctcgagcgtc atccgctcgt
     3121 ggcggaggtt gtgctcgatc ggctgcccgt cctggagcag gacgagcggc gccgcctcga
     3181 gcaccggccg cgcggcgcgg aaccggtaga cgatgtacga ggagagcacc gcgagcccgg
     3241 cgaacgtccc gaccgcgagg acggcgcccg tcatcgacat gtcgctctga gtcacgccct
     3301 gctgcatcag gtcgccgatc acgacgagca ggatcacgtc gaacggctcc agggaggaga
     3361 gctcgcgccg gccgatcacg cgcagcagga gggtgatgaa gaggaaggcg acggctgcgc
     3421 gaaggacgat gtccatggct cagggcagga tcgtcaccgt gcggtcggtg cgggcgacaa
     3481 gctcctcgcc gtcgtgcagc tcgacgctct gggagcggcg gccgacgttc gtcgggttga
     3541 cctgcagctg gaggtagacg accgccgtct cgccggccgc gagccggccg tacgagagcg
     3601 cgaggcgccc gtcgcggctc tcctcctccg tcggcgccgg ctcgatcgtg ttcacgtgca
     3661 tcgactcgag ccagccgcgg tcgaggacga gggtggcgtt ctcgagctcc tcctgcgtct
     3721 cgatcgtgat ccggcccatg aagaggacgc cgccgcggag cgcggtcggc gcctgcacct
     3781 cgatccgcgc ctgcgcgccg tctgcggcgg tcgtgacggg ccgctggccg aagacgttca
     3841 ggagggcgag cagcgcgagc agcgcgatcc cggcgagcac cgcgcggcgc acccagagct
     3901 cgaccgggtg ctgcgagtca cggctcttct cgaggacgag gaactgcggc gcgcccgcca
     3961 tgcggcgtta gttggcgggc gcgccttcaa tccctgccgg agctacggga gcaggcttcc
     4021 gtcgaagaac tcgaccacgc gtcgctcgta ctcggcgggc tgcgcctcga gcccgccggt
     4081 gtgcccggcg ccgggaacct cccagagctc cttcggctcc ccggccgcgg cgtagtagcg
     4141 cggctgccgc ccgttctcgt tcccgatccc gtgctccgcg ctgatcagga acacgggccg
     4201 cggcgcgatc agcccgatcc gctccacgat cgggggcggc gggccgtggt tcgagaacac
     4261 ggtgaccgcg gcggtgagga cggtctggag cggccccgcg aggatccgcc cggcgccctc
     4321 ggtgtcctgc tcgccgaccc gctcgccggc gccgtccgag acgatcgcgg cgaacgcgtc
     4381 ggacttggcg gccgtctcga ggaggatctc gccgccgatc gagtagccga tcgcgccgat
     4441 cctggccggg tcgacgcccg gttgcccggc gaggaacgcc gcgccggcga ggaggtcgcg
     4501 gtcgcccgcc cagcggacga tgtcgccttc gctcccgccc tggcctcgcg actcgaggag
     4561 gaggacgccg tagccgttgt cggcgagcat gcgcgcctcg tcggcccgcg tcgcgcccgg
     4621 gaagacgacg accgcggcgc cgttctccga cgggatgtgc caggcggcga ggtcgagcga
     4681 gtcgctcgtc gtcaccgtca ccggctcgaa cggtgcgccg ggcgcgggtt gcgacgaggt
     4741 gcggcccgtg tgcgtgtaga cgtacgcgag cgagacgggg aagacgacga gccagacgag
     4801 gacgaacgcg agcgccgtcc ccgctgcgac ggtgagggcg cggagggcgt accggcggcg
     4861 ccgggtgccg ccgcggcgcc gggcttgcca gagcgtcacc ggcccgctcg cgaggagcac
     4921 gacccctgcc acgagcgcga gcaggccggt gtagtgatcg ccggacgccg tgccgtcggc
     4981 caggtagtac gcgccgggca cgccgatcgc gatcgccagc gcgccgaagg tcatggccgt
     5041 ggccgcccgg gcgcccgcgc ggagacgcgg gtagaggtac gcgaccgccc cgaggaccgc
     5101 gatcggcacg agcccgctcg ccaggtggtc gccgggcgac gtgcccggct ccggccgcag
     5161 gtagttgtcg tccgcgacat gcagcgcgac gagcccgagc gcgaggcgga cgcgacccgt
     5221 ctcgctgagg gcgaacgcgc cgagccgggc gagcccgagc cgccacggcg acgcgcccgg
     5281 ggcgggaagg ctcccgtcgc tcacgacgcg ccttgcgcgc ccgtcgctcg tgccctggag
     5341 cgctcgagga ggacggccgc cgcgacgaag cagacgaggg cgatcggccc gatgggaggc
     5401 gggtgcaccg cgaaggccgc cgcgagcccc acgaggacgg tgatcgtcca gcccgcgccg
     5461 gctcgccgca gcgcgaccgc ggcggcgacc atcgccacga tccagccgag ggagccgagc
     5521 gcgagcgcga cggagatcgg atcgccgagg acggcgttcc ggttgagctc ctggatcgcg
     5581 tcgccgacgg cggcctgctc cgcgccggcc agcccgttcg cgtggtcgac gagcacgccg
     5641 gtggcgacgc cgaccgtcgc ctcgtacgcc gtgtagaaga cgaggaagaa cacgagcgcg
     5701 acccggctca cgagcgccgc gagcccgtgc aggccgtcca gcaggaggta ggcggcgatg
     5761 ccgaggaacg gcagggcgag cagcatcccg atgtggacga agagccacgc gtcgacgtct
     5821 ccgcggacgc cctcgtacac ggggtcgccg ccgggcggat ggaagaggag
//
LOCUS       DASTFC010000322         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1458704,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000322 DASTFC010000000
VERSION     DASTFC010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458704"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1021)
                     /locus_tag="VFO88_09510"
     CDS             complement(20..1021)
                     /locus_tag="VFO88_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545804.1"
                     /translation="MGRIGTWLLVVAVAALGIAATVDALRGGEAAPGPEAAETTDAEP
                     DPLADVEEPPAGWSRRAAAALEEGGARGALLVGDADCAVTVLEIPSLLVSRQGRPACG
                     FTTSPGGWIGAEPRVVAPGAEVIARCGEGDAETEIFGDGSQLLARVAGCGPAWTPDGR
                     LSVLRDGGLVAVSPCDADWSCEEMLVDRSGLARIFGRTPWSFRAPELLAAAWLSRETY
                     AVVVRDAEQGLSAIAVLRGAELVGGPPFVYENLEWLRASPQGSFAATRVGERGLVVVD
                     SNGSFVGTTFRAASAIAWSPDERWTAYATAEGVWLVPTGRGNRPLLLPLEAVDVAWR"
     gene            complement(1045..2217)
                     /locus_tag="VFO88_09515"
     CDS             complement(1045..2217)
                     /locus_tag="VFO88_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9545805.1"
                     /translation="MTIPAAEIEGRVARFQEALRAAELDGALVVQETDLVYLSGTGQS
                     AHLVVPAEGEPALFVRKTLERAREESPLARVEPLRSLRDLPGALASCGVEGGRLGLEL
                     DVLPAASYLAYARRLDRFELADCSLPLRRVRALKSTWELERIHVAAEQLSHVPEWMAR
                     HARPGMTEVELAAELERELRLAGHQGPIRFRAFNNELFYGSVLAGPSGAVPGSTETPV
                     VGPGPNVAVSKGASHRLIEEGEPILIDFAGAYEGYLADQTRTYSLGPLPRYLEEPYEA
                     AREILHRVAAEARPGVTGAQLYEKAMELAGDREGFMGWGDERVSFVGHGIGLEIDELP
                     LLARGWDEPLEAGMVFALEPKFVFPEVGAVGIENSYLVTGNGVERLTSAPEELIEL"
     gene            2237..>3118
                     /locus_tag="VFO88_09520"
     CDS             2237..>3118
                     /locus_tag="VFO88_09520"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9545806.1"
                     /translation="MILENGLIRTMDPSLPTARALAIAGEKIVGGVGTHESALASPEV
                     VDLRGLTVLPGFTDSHVHFAQWSLAQRQVRLEGARSLDEAVSRVAEAVRGAAPGRWLR
                     GTGWRSGEWSPEVEPTRGALDAVTGDVPVALMAKDGHSLWLNSAGLAAANGDLHVPGG
                     VVELDDRGEPTGVLREESAWHFRETYVSTPDEEWVDAMRAGVKLANARGVTAVHDKDG
                     WMGIARWWQTLRDEGALTLRVWQSIPWERLPEVEELGLRSGFGDDLLRLGYVKVFMDG
                     TLGSKTARLLDGSGVEIT"
BASE COUNT          405 a         1165 c         1177 g          371 t
ORIGIN      
        1 gtcggcaggc gccgcgccgt catcgccagg cgacgtcgac cgcctcgagc gggaggagga
       61 gcggccggtt cccgcgcccg gtcgggacga gccagacgcc ctcggcggtc gcgtacgccg
      121 tccagcgctc gtcgggcgac cacgcgatcg ccgaggcggc gcggaacgtc gtcccgacga
      181 agctcccgtt cgagtcgacg acgacgagcc cgcgttcgcc cacgcgcgtc gccgcgaagc
      241 tgccctgcgg gctcgcacgc agccattcga gattctcgta gacgaagggc gggccgccga
      301 cgagctcggc gccgcgcagc acggcgatcg cgctcagccc ctgctcggcg tcgcggacga
      361 cgaccgcgta cgtctcgcgc gagagccacg cggccgcgag gagctcgggg gcgcggaagc
      421 tccacggcgt gcggccgaag atccgcgcga ggccgctcct gtcgacgagc atctcctcgc
      481 aggaccagtc cgcgtcgcac ggcgagacgg cgacgagacc gccgtcccgc aggacgctca
      541 accgcccgtc cggcgtccac gccggcccgc agcccgcgac gcgcgcgagg agctgcgagc
      601 cgtcgccgaa gatctccgtc tcggcgtcgc cctcgccgca gcgcgcgatg acctcggcgc
      661 cgggcgcgac gacgcggggc tccgcgccga tccacccgcc cggggacgtc gtgaacccgc
      721 acgccggccg tccctggcgc gagacgagca ggctcgggat ctcgaggacg gtcaccgcgc
      781 agtccgcgtc gccgacgagg agcgccccgc gcgcgccgcc ctcctcgagc gcggccgcgg
      841 cccggcgcga ccagcctgcg ggcggctcct cgacgtcggc gagcgggtcg ggctcggcgt
      901 ccgtcgtctc cgccgcctcg ggcccgggcg ccgcctcgcc gccgcggagg gcgtccacgg
      961 tcgcggcgat cccgagcgcc gcgacggcca cgacgagcag ccatgtcccg atgcgtccca
     1021 cctgcctcgg gccacggtac ctcgctagag ctcgatcagc tcctccggcg cgctcgtcag
     1081 gcgctcgacg ccgttgccgg tcacgaggta cgagttctcg atcccgaccg cgccgacctc
     1141 ggggaagacg aacttcggct cgagcgcgaa gaccatcccg gcctcgagcg gctcgtccca
     1201 gccgcgcgcg aggagcggca gctcgtcgat ctcgagcccg atcccgtggc cgacgaacga
     1261 gacgcgctcg tcgccccacc ccatgaaccc ctcgcggtcg ccggcgagct ccatcgcctt
     1321 ctcgtagagc tgcgcgcccg tcacgcccgg tctcgcctcg gccgcgaccc ggtggaggat
     1381 ctcgcgcgcc gcctcgtacg gctcctcgag gtagcgcggg agcgggccga gcgagtacgt
     1441 ccgggtctgg tcggcgaggt acccctcgta cgcgccagcg aagtcgatca ggatcggctc
     1501 cccctcctcg atgagccggt gcgacgcgcc cttcgagacg gcgacgttcg ggccggggcc
     1561 gacgaccggc gtctccgtcg agcccggcac ggccccgctc gggccggcga ggacggagcc
     1621 gtagaagagc tcgttgttga acgcgcggaa gcggatcggc ccctggtggc ccgcgaggcg
     1681 aagctcgcgc tcgagctcgg cggccagctc gacctcggtc atcccgggcc gcgcgtggcg
     1741 ggccatccac tcggggacgt gcgagagctg ctcggccgcc acgtggatgc gctcgagctc
     1801 ccaggtcgac ttcagggctc ggacgcggcg gagcgggagc gagcagtcgg cgagctcgaa
     1861 ccggtcgagc cgccgcgcgt aggcgaggta cgaggcggcc gggaggacgt cgagctcgag
     1921 cccgagccgc ccgccctcga ccccgcacga ggcgagcgcg ccgggcaggt cgcggagcga
     1981 gcggagcggc tcgacgcggg cgagcgggga ctcctcccgg gcgcgctcga gcgtcttgcg
     2041 gacgaagagc gccggctcgc cctccgccgg gacgacgagg tgcgcgctct gacccgtgcc
     2101 ggagagatag acgaggtcgg tctcctgcac gacgagggcg ccgtcgagct cggccgcgcg
     2161 cagcgcctcc tggaagcgcg cgacgcgacc ttcgatctcc gccgccggga tggtcacggc
     2221 aatagtcttt cggatcgtga tcctcgagaa cggcctcatc cgcacgatgg acccgtcgct
     2281 cccgacggcg cgggcgctcg cgatcgcggg cgagaagatc gtcggcgggg tcgggacgca
     2341 cgagagcgcg ctcgcctcgc ccgaggtggt cgacctgcgc ggcctcaccg tcctgcccgg
     2401 cttcacggac tcgcacgtgc acttcgcgca gtggtcgctc gcccagcggc aggtgcgcct
     2461 cgagggcgca cgctcgctcg acgaggccgt ctcgcgggtc gcggaggcgg tgcgcggcgc
     2521 ggcgccggga cgctggctcc gcgggacggg ctggcggagc ggcgagtggt cgcccgaggt
     2581 cgagccgacc cgcggggcgc tcgacgcggt gaccggcgac gtccccgtcg cgctcatggc
     2641 gaaggacggg cactcgctct ggctcaactc ggcggggctc gccgccgcga acggcgacct
     2701 ccacgtgccc ggcggagtcg tcgagctgga cgaccggggc gagcccaccg gtgtcctgcg
     2761 ggaggagtcc gcatggcact tccgcgagac gtacgtctcg acgccggacg aggagtgggt
     2821 cgacgcgatg cgcgccggcg tgaagctcgc gaacgcgcgc ggcgtgaccg ccgtccacga
     2881 caaggacggc tggatgggga tcgcccgctg gtggcagacg cttcgcgacg agggcgcgct
     2941 cacgctgcgg gtgtggcagt cgatcccgtg ggagcggctc ccggaggtgg aggagctggg
     3001 cctgcgctcc ggcttcggcg acgacctgct gcggctcggc tacgtgaagg tcttcatgga
     3061 cgggacgctc ggctcgaaga cggcgcggct cctcgacggc tcgggcgtcg agatcacg
//
LOCUS       DASTFC010000323         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_947726,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000323 DASTFC010000000
VERSION     DASTFC010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947726"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFO88_09525"
     CDS             complement(<1..909)
                     /locus_tag="VFO88_09525"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET9545807.1"
                     /translation="MSELATLPGLESVESSAKRDHWISHGPEGRLMLFSGRSNLDLAG
                     AIGEKLGIELGEVKLKTFSNGEVYCRYMESIRGADVFLVQSCAGAGVNDALVELLVMI
                     DAARLASANRITAVMPWYPYSRQDKKSAPREPITARLIAGFLEAAGADRVLTMDLHAG
                     QIQGFFRIPVDHMTALPLFAQYYRDKGLYGDRVVAVAPDPGRGKMTTRFAEFLESDVA
                     YMSKTRPEHDVAVVTEVVGRERVAGKVAILSDDLIVTGSTLVSGAKALLEAGATAVYA
                     CATHGLFPPDALASLGESELTEIVVTD"
     gene            complement(902..2257)
                     /gene="glmU"
                     /locus_tag="VFO88_09530"
     CDS             complement(902..2257)
                     /gene="glmU"
                     /locus_tag="VFO88_09530"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET9545808.1"
                     /translation="MTGGSNPPAPAIAAVVMAAGLGTRMRSRRPKHLHELLGRRMLDW
                     ALDAVIPLGPSPLVVVVSPDTRAELDGTLPAGATLALQDEPRGTGDAAAAGLDALGGF
                     DGDVLVVPGDAPLLAPELVRELVETHRREGASVTLLSIETGKPLPYGRVVRDAEGRVR
                     RIVEEGDATEEERAIRELNASVYAFAAGDLRDALARLKPDNAQGELYLTDAVRLVADA
                     GRPVATVRTTDVDAAQGVNTRVELAEAAAALRERILRAHMLAGVTITDPATTWIDGGV
                     EIEPDAVIGPFTVLQGRTVVGAEAQVGPHVVARDAEIGPRATVGPFAYLRPGARLGAD
                     AKVGTFVEVKNAEIGERSKVPHLSYIGDAEIGEDTNIGAGAITANYRPEMGSGKQRTR
                     IGSNVHTGSDNVFVAPVEIGDNAWIGAGSTITDDVPPGALAIARARQVVKERYGDKRD
                     E"
     gene            complement(2224..2297)
                     /locus_tag="VFO88_09535"
     tRNA            complement(2224..2297)
                     /locus_tag="VFO88_09535"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2263..2265),aa:Gln,seq:ttg)
BASE COUNT          301 a          838 c          851 g          340 t
ORIGIN      
        1 gtcggtgacg acgatctccg tcagctcgct ctcgccgagg ctcgcgagcg cgtcgggcgg
       61 aaagaggccg tgcgtcgcgc acgcgtacac ggcggtcgcg ccggcctcga gcagcgcctt
      121 cgcgccggag acgagcgtcg aaccggtcac gatcaggtcg tcgctcagga tcgcgacctt
      181 gccggccacg cgctcgcggc cgacgacctc ggtcacgacc gccacgtcgt gctccggccg
      241 cgtcttcgac atgtacgcga cgtcggactc gaggaactcg gcgaagcggg tcgtcatctt
      301 cccgcggccc gggtccgggg cgacggcgac gacgcggtcg ccgtagaggc ccttgtcgcg
      361 gtagtactgc gcgaagagcg ggagcgcggt catgtggtcg accgggatcc ggaagaagcc
      421 ctggatctga cccgcgtgga ggtccatcgt gaggacgcgg tcggcgccgg ccgcctcgag
      481 gaacccggcg atcaggcgtg cggtgatcgg ctcgcgcggc gcggacttct tgtcctgccg
      541 cgagtacggg taccagggca tgacggccgt gatccggttc gcggaggcga gccgggctgc
      601 atcgatcatg acgaggagct cgacgagcgc gtcgttcacg cccgcgcccg cgcacgactg
      661 gacgaggaag acgtccgcgc cgcggatcga ctccatgtag cggcagtaga cctcgccgtt
      721 cgagaacgtc ttcagcttga cctcgccgag ctcgatcccg agcttctcgc cgatcgcgcc
      781 cgcgaggtcg aggttcgagc ggccggagaa gagcatgagc cttccctccg ggccgtgcga
      841 gatccagtgg tcgcgcttcg cgctcgactc gacgctctcg aggcccggaa gggtggccag
      901 ctcactcatc tcgcttgtct ccgtagcgct ctttgacgac ctgtcgagcc cgggcgatcg
      961 cgagggctcc tggtgggacg tcgtccgtga tggtggagcc ggccccgatc catgcattgt
     1021 caccgatctc gacgggagca acgaagacat tgtcgctccc ggtgtggacg ttactcccga
     1081 ttcgcgtccg ctgcttgccc gaccccatct cgggccggta gttcgcggtg atcgcgcccg
     1141 cgccgatgtt cgtgtcctcg ccgatctcgg cgtccccgat gtacgagagg tgcggcacct
     1201 tcgagcgttc cccgatctcg gcgttcttca cctcgacgaa cgtgccgacc ttcgcgtcgg
     1261 cgccgagccg ggcgcccggg cggaggtacg cgaacgggcc gacggtggcg cgcgggccga
     1321 tctccgcgtc gcgggcgacg acgtgcgggc cgacctgcgc ctcggcgccg acgaccgtcc
     1381 ggccctggag gacggtgaac gggccgatca ccgcgtcggg ctcgatctcg accccgccgt
     1441 cgatccaggt cgtcgccggg tcggtgatgg tcacgccggc gagcatgtgc gcgcggagga
     1501 tgcgctcgcg gagggccgcg gccgcctcgg cgagctcgac gcgcgtgttc acgccctgcg
     1561 cggcgtccac gtcggtcgtc cgcacggtcg cgaccggccg gcccgcgtcg gcgacgaggc
     1621 gcacggcgtc ggtgaggtag agctcgccct gcgcgttgtc cggcttcagc cgggcgagcg
     1681 cgtcgcgcag gtcgccggcc gcgaacgcgt acaccgacgc gttcagctcc cggatggccc
     1741 gctcctcctc ggtcgcgtcg ccctcctcga cgatccgccg cacgcggccc tccgcgtcgc
     1801 ggacgacgcg tccgtacggg aggggctttc cggtctcgat cgagaggagc gtgaccgagg
     1861 cgccctcccg gcggtgcgtc tcgacgagct cgcggacgag ctcgggcgcg aggagcgggg
     1921 cgtcgccggg gacgacgagc acgtcgccgt cgaagccgcc gagcgcgtcg agccccgcgg
     1981 cggccgcgtc gccggtcccg cgcggctcgt cctgcagcgc gagcgtcgcg ccggcgggga
     2041 gcgttccgtc gagctccgcg cgcgtgtcgg gcgagacgac gaccacgagc gggctcggcc
     2101 ccagcggtat gaccgcgtcg agcgcccagt cgagcatccg ccggccgagc agctcgtgga
     2161 ggtgcttggg ccgccgcgac cgcatccgcg tgccgagccc cgcggccatc acgacggcgg
     2221 cgatggctgg ggcgggagga ttcgaacctc cggtcacggg accaaaaccc gctgccttac
     2281 cacttggcta cgccccaccg gcgccagcgt accgccatca gcggcgcccg
//
LOCUS       DASTFC010000324         8486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_338402,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000324 DASTFC010000000
VERSION     DASTFC010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338402"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFO88_09540"
     CDS             <1..445
                     /locus_tag="VFO88_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET9545809.1"
                     /translation="PPEKVLGLAEEAIELGAHEVVFADTIGVATPTQVRHVVVEGVKF
                     ARPVGVHLHNTRSTGFANAWAALEWGASIFDASVGGVGGCPFAPRATGNIATEDLVYL
                     LRGEGVDTGIDLDALIGAAEWLEGVLGRELPGQVYRAGSFVPVSG"
     gene            complement(442..1116)
                     /locus_tag="VFO88_09545"
     CDS             complement(442..1116)
                     /locus_tag="VFO88_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET9545810.1"
                     /translation="MAPLNSELLGEARAARDRLLELQRDLEKTRADYNHAVRRLHAEG
                     GSLREIAEDLGLSHQRVHQIVDAGEGGAARTGPRRRFPWAFERFTRRARRIVALAQEE
                     AAELGHGRVGTEHLLLGLVRAEDEAVAPVLSEAGITLERLRELVRALDPGPRRRSPFS
                     RAAKRALEQSLREAQALGDRFIGPEHVLLGLLADERAGSLALVRELGADPDALRAAVL
                     ERRAAG"
     gene            complement(1320..2858)
                     /locus_tag="VFO88_09550"
     CDS             complement(1320..2858)
                     /locus_tag="VFO88_09550"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9545811.1"
                     /translation="MSEVIHAPEPALEEEQGPRRISHWIGGRIVPGESGRTGPVFNPA
                     TGEQTGEVDFATREEVDRAVAAAREAFPAWRALSLSKRSDLFFRIRALVHEHREDIAR
                     FLTLEHGKVLSDALGEVARGLEVVDHACGIPTLLQGRFSEQASTGIDVYSIRQPLGVC
                     AGITPFNFPAMVPMWMWAPALACGNTFVLKPSEKDPSASLFVAELLQEAGVPDGVFNV
                     VMGDKVAVDSLLEHPDVAGISFVGSTPIARYIYETGAKHGKRVQALGGAKNHMVVLPD
                     ADIDMAADAAVSAAYGSAGERCMAVSVVVAVGDVGDRLVEAIKERLPKVKIGPGLDPS
                     SEMGPLVTREHRDKVASYIESGREQGATVVADGRELEVDGDGFFLGVSLLDDVKPEMD
                     AYKDEIFGPVLSVVRAETYDEAVRLANENPYGNGVAIFTRDGGAARQFQFEVEAGMVG
                     VNVPIPVPVAYYSFGGWKASLFGDSHVYGPEGINFYTRGKVVTSRWPDPATSVVDLGF
                     PQTR"
     gene            3142..4269
                     /locus_tag="VFO88_09555"
     CDS             3142..4269
                     /locus_tag="VFO88_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545812.1"
                     /translation="MAEAADPKMELTPADAAAIEDARAHAVEELLSPEAPHSNVVGVA
                     AGVKWRNGEPTGDPAVIALVTHKVDKSELAGDDLVPAKIGTTRTDVLEIGFPMIERAH
                     VHAPAAVNGSTTVEEAEQVLVVPQVPLFGAEALARRARPAEGGYSVGHFKITAGTIAT
                     CVYDLLPGAATGSQPKPGVGMPQRFYILSNNHVLANVNAAMVGDPILQPGPFDGGTFP
                     TDLIARLSRFIPITLEPPTPRILHRNLVDAAVAEGQFHDLDREIYWGGYVRGWRRRAN
                     VAVGTTVQKTGRTTNFTTGRITAINATVDVGFGGGRIGRFQDQIITTNMSAGGDSGSL
                     VVTPDEVAVGLLFAGSSVATIANQIENVRSLLRVEVAEQIL"
     gene            4343..4720
                     /locus_tag="VFO88_09560"
     CDS             4343..4720
                     /locus_tag="VFO88_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9545813.1"
                     /translation="MIAAELIRIARENEHFRREVATAERSQVVVMTISPGEEIGEEVH
                     EGIDQILLFVEGECEALVGEEVRIVRADEMVLVPGGTRHNFVNTGDGPLRLVTVYSPP
                     EHPAGTVHRTKAEADAAEAEHGH"
     gene            complement(4717..5352)
                     /locus_tag="VFO88_09565"
     CDS             complement(4717..5352)
                     /locus_tag="VFO88_09565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9545814.1"
                     /translation="MPEPVELYFDRRARAYDRRYDHRRLQRIIRPSLLRGREAALALA
                     RSLGAPRVLEAGCGPGRLGEALLAAGVAEYVGVDVSGAMVERAQARLARFADRARVIR
                     APFAAAPVEGPFDLVVALGLFDYVDDPEATLGRMTDECAGVLLATFPRWDVVRDPVRT
                     LVYRYAYGCRIRHYDERRLAALLDRCGLGDATVETRRTGFRVTARVVPPPS"
     gene            complement(5355..6044)
                     /locus_tag="VFO88_09570"
     CDS             complement(5355..6044)
                     /locus_tag="VFO88_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET9545815.1"
                     /translation="MSARRDATRILVVCAHPDDEVLGMGATIARHTLAGDRVHVLCLS
                     EGSSAQYPDEPDRRRERTDAGRRAAELLGVEELVQLEHPDERLDTVPQLELNRAIESQ
                     IARFAPEIVYSVHGDVNSDHRAVFRSTAVATRSTPAQSVRRVLAFAIPSSIEWTPTQI
                     ASFAPTWFVDVGTTFERKLEAFACYETEARPYPHPRSVEGLRAYAAFHGAAAGCELAE
                     PFVLVRNVVRG"
     gene            complement(6041..7144)
                     /locus_tag="VFO88_09575"
     CDS             complement(6041..7144)
                     /locus_tag="VFO88_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9545816.1"
                     /translation="MSAGGVSPSAVLGREVVELGEERFAGYLAGARVLVTGAGGSLGR
                     ALARRLTGIGVETLVLVDQGEAALAEAHDELGHLDPGVRVVAVLADLRSASRSRALLA
                     EHAPEAVFHAAAYKVVPLVEASPLEAVATNVLATEALVDASLEAGVARFVLFTTDKAV
                     RPVSVLGRTKAAAECIVGTAARRADLARFACIRLGNVVDSAGSMLPLVRRRLAADRPV
                     PITDARVTRYLMTADEAVALAVAAGGLAGSGEVFRLDMGPPVRVLDAARTVAEASANG
                     HGATIELVGLRPGEKLHEELDEPGEPVERVGREPIFRSAARYADADWLAGRLDGLRAA
                     VTGADALAARRELARLVAGIRIADRLPEPVGPV"
     gene            complement(7148..8287)
                     /locus_tag="VFO88_09580"
     CDS             complement(7148..8287)
                     /locus_tag="VFO88_09580"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9545817.1"
                     /translation="MTAPPSVVADEIGLSGAYLDERDEQLVLEVLRSGRLALGPMTER
                     FERLLAERVGAHRVAALSSGTAGLHLGVKLAGVRPGDEVVTSPLSFIASANAILYEGG
                     IPVFVDVDPRTLNVDPAAVEAAITPRTRAVLPVALFGYPLEYDGLLDVTGRHGLAVVE
                     DAAEALGTTYRGRPVGSFGHPTVFAFYPNKQMTTGEGGAIAVASDDEWELVKSLSNQG
                     RADRGETFLHTRLGYNYRLDELSAALGVAQLEKLDLLLELRSRVAARYDALLADLEGV
                     TTLCPDDADRRRSWFVYVVFVEEPHDRDTIMARLAARGVSSKPYLPALHLQPLYRERF
                     GFRPGMFPVAERAGARGLALPFHAGLSEDAQARVVAALTAALRDG"
BASE COUNT         1218 a         3081 c         3116 g         1071 t
ORIGIN      
        1 tccgccggag aaggtgctcg ggctcgccga ggaggcgatc gagctcggcg cgcacgaggt
       61 cgtcttcgcc gacacgatcg gcgtcgcgac gccgacgcag gtgcgccacg tcgtcgtgga
      121 aggcgtgaag ttcgcccggc ctgtcggggt ccacctgcac aacacgcgct cgaccggctt
      181 cgcgaacgcc tgggcggcgc tcgagtgggg ggcctcgatc ttcgacgcgt cggtcggcgg
      241 ggtcggcggc tgcccgttcg cgccgcgcgc gaccggcaac atcgcgaccg aggatctcgt
      301 gtacctcctg cgcggcgaag gcgtggacac ggggatcgac ctggatgccc tcatcggcgc
      361 cgccgagtgg ctcgagggcg tgctcgggcg cgagctgccc ggccaggtct accgggccgg
      421 ctccttcgtg ccggtctccg gctagccggc cgcgcggcgc tcgaggaccg cggcgcgcag
      481 cgcgtcggga tcggcgccga gctcgcggac gagggcgagc gacccggcgc gctcgtcggc
      541 cagcaggccg aggaggacgt gctccgggcc gatgaagcgg tcgcccagtg cctgggcctc
      601 gcggaggctc tgctcgagtg cccgcttggc cgcccgcgag aacggcgagc gccgccgcgg
      661 gccggggtcg agcgcgcgga cgagctcccg cagccgctcg agggtgatgc ccgcctcgga
      721 gagcacgggc gcgacggcct cgtcctccgc ccggacgagg ccgaggagca ggtgctcggt
      781 gccgacgcgg ccgtggccga gctcggcggc ctcctcctgc gcgagcgcga cgatccggcg
      841 ggcgcggcgg gtgaagcgct cgaaggccca cgggaaccgt cgccgcgggc cggtgcgggc
      901 ggcgccgccc tcgccggcgt ccacgatctg gtggacgcgc tggtggctca ggccgagatc
      961 ctcggcgatc tcgcgcagcg agccgccctc ggcgtggagg cgacggacgg cgtggttgta
     1021 gtcggcgcgc gtcttctcga ggtcgcgctg gagctcgagc agccggtcgc gcgccgcacg
     1081 ggcctcgccc aggagctcgg agttcagcgg agccacaccg tctacggtac tagacagcac
     1141 gcagagtgtc aatcccggta gacacgctcg ccgcgaaacg cgagcagccc cccatcgggg
     1201 ggcgcgagcg ccccggaacg aacgctatcc gggacgggcg caccggtccg tgaccggacc
     1261 gtgccggatg tgtgccgatc cgtcccgaga cgggcggcgg gcccgctcgc cgaaccgcgc
     1321 taccgcgtct gcgggaagcc cagatcgacg acgctcgtcg cgggatccgg ccagcgcgac
     1381 gtgacgacct tcccgcgcgt gtagaagttg atcccttccg gcccgtagac gtgcgagtcg
     1441 ccgaagaggg acgccttcca cccgccgaac gagtagtacg cgaccggcac cgggatcggg
     1501 acgttcaccc cgaccatccc ggcctcgacc tcgaactgga actgccgcgc cgcgccgccg
     1561 tcgcgcgtga agatcgcaac gccgttgccg tacgggttct cgttcgcgag ccgaaccgcc
     1621 tcgtcgtacg tctccgcgcg gacgaccgag agcaccggcc cgaagatctc gtccttgtac
     1681 gcgtccatct ccggcttgac gtcgtccagg agcgagacgc cgaggaagaa gccgtccccg
     1741 tccacctcga gctcgcgccc gtcggcgacg accgtggcgc cctgctcgcg gccggactcg
     1801 atgtacgagg cgaccttgtc gcggtgctcg cgcgtgacga gcgggcccat ctcggaggac
     1861 gggtcgagcc ccggcccgat cttcaccttc gggagccgct ccttgatcgc ctcgacgagc
     1921 cggtcgccga cgtcgccgac cgcgacgacg accgacacgg ccatgcaccg ctcgccggcc
     1981 gagccatacg cggcgctgac cgcggcgtcc gctgccatgt cgatgtcggc gtccgggagg
     2041 acgaccatgt ggttcttggc cccgccgagc gcctggacgc gcttgccgtg cttcgcgccc
     2101 gtctcgtaga tgtagcgcgc gatcggcgtc gagccgacga acgagatccc ggcgacgtcc
     2161 gggtgctcga ggagcgagtc gaccgccacc ttgtcgccca tgacgacgtt gaagacgccg
     2221 tccggaacgc cggcctcctg gagcagctcc gcgacgaaga gcgacgccga cgggtccttc
     2281 tcggacggct tcaggacgaa cgtgttcccg catgcgagcg ccggcgccca catccacatg
     2341 ggcaccatcg ccgggaagtt gaacggcgtg atccccgcgc agacgccgag cggctgacgg
     2401 atcgagtaga cgtcgatccc cgtcgacgcc tgctcggaga agcgcccctg aagcagcgtc
     2461 gggatcccgc acgcgtggtc gaccacctcg agcccgcgcg cgacctcccc gagcgcgtcc
     2521 gagagcacct tgccgtgctc gagcgtgagg aagcgcgcga tgtcctcgcg gtgctcgtgc
     2581 acgagcgcgc ggatccggaa gaagaggtcg gagcgcttcg agagcgagag cgcccgccag
     2641 gcggggaacg cctccctggc tgcggcgacg gcgcggtcga cctcctcgcg cgtcgcgaag
     2701 tccacctcgc ccgtctgctc gccggtggcc gggttgaaca ccgggccggt gcggcccgac
     2761 tccccgggca cgatccggcc tccgatccag tggctgatcc gcctggggcc ctgctcctcc
     2821 tcgagcgccg gctcgggcgc gtggatgacc tcgctcatgt cgcctcctcg tcgtctgccg
     2881 caagggtacc ggccgccggt gaacgtcaca cttgacacgt tgagcgcgcg ggcgtaccgt
     2941 cggccccagg aagggagcga ggggcggatg gccgctgttc gacagtcggc ctgcggcagg
     3001 ggcggagggc gccggaaacg ggtcaggcgg cgccctgcgc gtggtccccc gcgtgcgcgc
     3061 cgagacgcga tcgagggtca cgcggtcgcc cgcccgggga gctgggcgcg cccgtgatcg
     3121 aactccccga ggaaggacga catggctgag gcagcagatc cgaagatgga gctcactccc
     3181 gcggacgcgg cggcgatcga ggacgcccgc gcccacgcgg tcgaggagct gctctccccc
     3241 gaggcgccgc actcgaacgt cgtcggcgtc gctgcggggg tcaagtggcg aaacggcgag
     3301 ccgaccgggg atccggccgt catcgcgctc gtcacgcaca aggtggacaa gagcgagctc
     3361 gccggggacg acctcgttcc ggccaagatc ggcaccacgc gcacggacgt cctcgagatc
     3421 ggctttccga tgatcgagag ggcgcacgtg cacgccccgg cagccgtgaa cggctcgacg
     3481 accgtcgagg aggcggagca ggtgctggtc gttccccagg tgcctctctt cggcgcggag
     3541 gcgctcgccc gccgcgcccg cccagcggag ggcggctact cggtcggcca cttcaagatc
     3601 accgccggga cgatcgcgac gtgcgtctac gacctgctcc cgggggcggc gacggggagc
     3661 cagccgaagc cgggcgtcgg gatgccgcag cggttctaca tcctgtccaa caaccacgtg
     3721 ctcgcgaacg tgaacgccgc catggtcggc gacccgatcc tccagccggg gccgttcgac
     3781 ggcggcacgt tcccgaccga cctgatcgcg cggctctcgc ggttcatccc gatcacgctc
     3841 gagccgccga cgccgcggat cctccaccgc aacctcgtgg acgcggcggt cgccgagggc
     3901 cagttccacg acctcgaccg ggagatctac tggggcggct acgtccgcgg ctggcggagg
     3961 cgggcgaacg tcgccgtcgg gacgaccgtc cagaagaccg gccggacgac gaacttcacg
     4021 accggccgga tcacggcgat caacgcgacg gtggacgtcg ggttcggcgg cggccggatc
     4081 ggccgcttcc aggatcagat catcacgacg aacatgtccg cgggcgggga ctccggctcg
     4141 ctcgtcgtga cgccggacga ggtggccgtc ggcctcctct tcgcgggctc gtcggtggcg
     4201 acgatcgcga accagatcga gaacgtccgg tcactcctcc gcgtggaggt ggccgagcag
     4261 atcctctagg ccgatcggag cggggggttg gggcccggcc ggacggcggc cgggccccgc
     4321 gcgtgctggt acggtgacgg gcgtgatcgc cgccgagctc atcaggatcg cgcgcgagaa
     4381 cgagcacttc cggcgcgagg tcgcgaccgc cgagcggagc caggtcgtcg tcatgacgat
     4441 ctcgccgggc gaggagatcg gcgaggaggt tcacgagggg atcgaccaga tcctcctctt
     4501 cgtcgagggc gagtgcgagg cgctcgtcgg cgaggaggtg cggatcgtcc gcgccgacga
     4561 gatggtgctc gtccccggcg gcacgcggca caacttcgtg aacaccggcg acgggccgct
     4621 gcgcctcgtc accgtctact ccccgcccga gcacccggcg ggcacggtgc accgcacgaa
     4681 ggccgaggcc gacgccgccg aggccgagca cggccactag ctcggcggcg gaacgacgcg
     4741 ggcggtgacg cggaatccgg ttcgccgcgt ctcgacggtc gcgtcaccga ggccgcagcg
     4801 atcgaggagc gcggcgagcc gtcgctcgtc gtagtgccgg atccggcacc cgtacgcgta
     4861 ccggtagacg agcgttcgca ccggatctcg cacgacgtcc cagcgcggga aggtcgcgag
     4921 gaggacaccc gcgcactcgt cggtcatccg tccaagcgtc gcctccgggt cgtcgacgta
     4981 gtcgaagagc cccagcgcga cgacgaggtc gaacggcccc tcgacgggag ccgctgcgaa
     5041 cggggcgcgg atcacgcgcg cacgatcggc gaagcgtgcg agccgcgcct gcgcccgttc
     5101 caccatcgct ccggagacgt ccacgccgac gtactcggcg acacccgcgg cgagcaaagc
     5161 ctcgcccagc cggccgggcc cgcagccggc ctcgaggacg cgaggtgcgc cgagcgagcg
     5221 cgcgagcgcg agagccgctt cgcggccgcg aaggagcgac ggacggatga tgcgttgcag
     5281 ccgcctgtgg tcgtaccgcc ggtcgtaggc gcgtgcccgc cggtcgaagt agagctcgac
     5341 gggctcgggc acgatcagcc gcgaacgacg tttcgcacga ggacgaacgg ctcggccagc
     5401 tcgcagccgg ccgccgcgcc gtggaacgcc gcgtacgcac gaagcccctc cacggagcgg
     5461 ggatgcggat acggccgtgc ctcggtctcg tagcacgcga acgcctcgag cttgcgctcg
     5521 aacgtcgtcc cgacgtccac gaaccacgtc ggcgcgaacg aggcgatctg cgtcggcgtc
     5581 cactcgatgc tcgacgggat cgcgaacgcg aggacgcggc gaaccgactg ggccggcgtg
     5641 ctcctcgtgg cgaccgccgt cgaacggaag accgcccggt ggtcgctgtt cacgtcgccg
     5701 tggaccgagt acacgatctc gggcgcgaaa cgggcgattt ggctctcgat cgcgcggttg
     5761 agctcgagct gggggacggt gtccagccgc tcgtcgggat gctcgagctg gacgagctcc
     5821 tcgactccga gcagctcggc cgccctgcgc ccggcgtcgg tcctctcccg cctccggtcc
     5881 ggctcgtcgg gatactgcgc ggagctgccc tcgctcagac agaggacgtg cacccggtca
     5941 ccggcgagcg tgtgccgcgc gatcgtcgcg cccatgccga gcacctcgtc gtccggatga
     6001 gcgcagacga cgaggatccg ggtcgcgtcc cggcgcgcgc tcacaccggg ccgacgggct
     6061 cgggcaaccg gtcggcgatc cggatgcctg cgacgagccg ggcgagctcc cggcgggccg
     6121 cgagagcgtc cgccccggtc acggcggcac ggaggccgtc gagccggccg gcgagccagt
     6181 ccgcgtccgc gtagcgggcc gccgaccgga agatcggctc gcgcccgacc cgttcgaccg
     6241 gctctccggg ctcgtcgagc tcttcgtgga gcttctcgcc cggccggagc ccgacgagct
     6301 cgatcgtcgc cccgtggccg ttcgcgctcg cctccgcgac cgtgcgggcg gcgtccagca
     6361 cacgcaccgg gggccccatg tcgagccgga acacctcgcc gctcccggcc agtccgcccg
     6421 ccgcgacggc gagcgcgacg gcctcgtcgg ccgtcatgag gtagcgcgtg acgcgcgcgt
     6481 ccgtgatcgg gacgggccgg tcggcggcga ggcggcgccg gacgagtgga agcatgctcc
     6541 cggccgagtc gacgacgttg ccgagccgga tgcacgcgaa gcgcgcgaga tccgcccggc
     6601 gggcggcggt gccgacgatg cactccgcgg cggccttcgt ccggccgagc acgctcacgg
     6661 gccggacggc cttgtccgtc gtgaagagga cgaaccgcgc gacgccggcc tcgagcgacg
     6721 cgtcgacgag cgcctccgtc gcgaggacgt tggtggcgac ggcttcgagc gggctcgcct
     6781 cgacgagcgg gacgaccttg tacgcggccg cgtggaagac ggcctcgggc gcgtgctcgg
     6841 cgagcagcgc acgggaccgc gaagccgacc gcaggtcggc cagcacggcg acgacgcgta
     6901 cgcccgggtc gaggtgaccg agctcgtcgt gcgcctccgc gagcgccgct tcgccctggt
     6961 cgaccaggac gagcgtctcg accccgatgc cggtgagccg gcgcgcgagc gcgcggccga
     7021 gcgacccccc ggcacccgtg acgagcacgc gcgcccccgc gagatagccg gcgaaccgct
     7081 cctcgccgag ctccaccacc tcgcggccga gcaccgcgct cggcgagacg cctccggcgc
     7141 tcacgcgtca gccgtcccga agcgcggcgg tgagcgctgc gaccacgcgt gcctgggcgt
     7201 cctcggagag gccggcgtgg aacggaagcg cgagcccccg cgcgccggcc cgctcggcga
     7261 cggggaacat cccgggccgg aacccgaacc gctcccggta gagcggctga agatggagcg
     7321 cgggcaggta gggcttcgag gagacccccc gtgcggcgag ccgagccatg atcgtgtccc
     7381 ggtcgtgcgg ctcctcgacg aagacgacgt agacgaacca cgagcgccgg cggtcggcgt
     7441 cgtccgggca gagcgtcgtc acgccctcga ggtcggcgag gagggcgtcg tagcgcgcag
     7501 cgacccgcga gcggagctcg aggagtagat cgagcttctc gagctgcgcc accccgagcg
     7561 cggcggagag ctcgtcgagc cggtagttgt agccgagccg cgtgtggagg aacgtctcgc
     7621 cgcgatccgc gcggccctgg ttcgagaggg atttcacgag ctcccactcg tcgtcgcttg
     7681 cgaccgcgat cgctccccct tcgcccgtcg tcatttgctt gttcggatag aacgcgaaca
     7741 ccgtcgggtg gccgaacgag ccgaccggtc gaccccggta ggtcgtcccg agcgcctccg
     7801 ccgcgtcctc gacgaccgcg agcccgtgcc gcccggtcac gtcgagcagc ccgtcgtact
     7861 cgagcggata gccgaagagc gcgacgggaa ggacagcgcg cgttcggggc gtgatcgcgg
     7921 cttccacggc cgccgggtcg acgttcagcg ttctcgggtc gacgtcgacg aagaccggga
     7981 tcccgccctc gtagaggatc gcgttggccg acgcgatgaa cgagaggggg ctggtcacga
     8041 cctcgtcccc cggccggacg cccgcgagct tgaccccgag gtggagcccg gccgtcccgc
     8101 tcgagagcgc ggccacccga tgggcgccga cccgctcggc gagcaggcgc tcgaaccgct
     8161 ccgtcatcgg gccgagcgcg agcctgccgg accggagcac ctcgaggacg agctgctcgt
     8221 cccgctcgtc gaggtaggcg cccgacaggc cgatctcgtc tgccaccacg gacgggggcg
     8281 ccgtcatggc gggataatga caggtcgcac aagccgggca aaacgccccg cccgtaagct
     8341 ccgtcccgct actcgagcgg cttcgacatg acgactgcgt cctcgcggta gccgagcgcg
     8401 cggtagagcc cccgcgcgac ctcgttgccg ccgaagacgt tcagctcgat ccgcggtgcg
     8461 ccgagacggc gcgcctcctc ctcgag
//
LOCUS       DASTFC010000325         4937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_882438,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000325 DASTFC010000000
VERSION     DASTFC010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882438"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFO88_09585"
     CDS             complement(<1..813)
                     /locus_tag="VFO88_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET9545818.1"
                     /translation="MDRRHGIGLGEAARVWALVGLNSFGGPAGQIAVMHRMLVEERRW
                     FGERRFLHALNYCMLLPGPEAQQLATYFGWLLHGVRGGLIAGSLFVLPGFALILALSL
                     IYAGHGDTALVEAVFYGVKPAVLAIVAYAVVRIGSRVLGNRVLVGLAAAAFVAIFFFD
                     VPFPLIVAGAAVVGWLGGRRRPDAFTGALADEDGRDDDEEPRVRPSLRRGAGVLALGL
                     ALWWGPVLALAAWLGRESVYVQEGTFFSVVALVSFGGAYAVLAYVAQEAVQTY"
     gene            842..1396
                     /locus_tag="VFO88_09590"
     CDS             842..1396
                     /locus_tag="VFO88_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET9545819.1"
                     /translation="MARAPSEEPEGLVYRPNVLSAEEERALLGVLAGLRFDPIVIRGQ
                     AAKRTARHYGLDYDYESRAPRPGEPVPDWLLPARARAAGLAGVEAEELVEILVQRYPP
                     GSTIGWHRDAPAFDLVVGLSLGAPARLRLQRGKGERRRVWELVAEPRSGYVLAGEARR
                     SWEHSIPPTKELRHSITFRTLRRR"
     gene            1455..2621
                     /locus_tag="VFO88_09595"
     CDS             1455..2621
                     /locus_tag="VFO88_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9545820.1"
                     /translation="MKPVPFVAALLASGLVGGGVAFAIGSTVWDGETTTVIRESAPAD
                     LQAAAFESDGAASTVGEVYARAAPGVVQVTSSRVTQGFFGPQPGEALGSGFVIDKDGH
                     IVTNYHVIEGADEILVNFSQDDGVAAELVGVDPSTDLALLKIQEHRRALTPIPLGDSS
                     TVRVGDQVIAIGNPFGLDRTVTAGIVSALQRQILSPSNFTIDKVIQTDAAINQGNSGG
                     PLLNADGDVIGVNTQIATAGSAEGNVGIGFAVPVNTVKTVVGELLESGRVEHSYLGVS
                     MQDLTPDVARYVNVPERGALVAQVYPGSPAEEAGLQGAQQTVVIDGQSYAVGGDVIVR
                     ADGRTIRTSDDLRQIVAAKKPGDELDLGILRGEEEVEVTVTLGTLPDVSADTRG"
     gene            2645..3709
                     /locus_tag="VFO88_09600"
     CDS             2645..3709
                     /locus_tag="VFO88_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HET9545821.1"
                     /translation="MIPHAFGERDGLTLGIEEELFVVDAESLEPAPVPPEVLDGERCK
                     PELFRAVVELTTGVCASVDEAAAELEELRSGARRRAADAGLALAATGTWPTAVAQEQE
                     ITPDPGYLEFVEYAGSSARRQFCSGLHVHVGVASPEACMAALERVLPWLPVVLAASAN
                     SPYLAGAETGLASTRAEILALLPRSAGPPVFGSYEEWERFAERLVELGLADRYTRIWW
                     DIRPHPTFGTLEVRTPDQPTRLEVTVAFAALVQALVATANGTPPEAPADRGLYAQNRW
                     AAARFGAQAELVHPDGSHVVRADELLAELLERVAPAAERLGTAVYLAPLEGLVQADTQ
                     LEHGRREGLRSLCDGLVAAT"
     gene            3719..3931
                     /locus_tag="VFO88_09605"
     CDS             3719..3931
                     /locus_tag="VFO88_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET9545822.1"
                     /translation="MAVQSDTIQVTGIRCERCVMRLGKALEGLEGLEGANATLMGQVS
                     LTWDDERVERQAIVERMSRAGFRPVE"
     gene            3928..>4937
                     /locus_tag="VFO88_09610"
     CDS             3928..>4937
                     /locus_tag="VFO88_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET9545823.1"
                     /translation="MTSAALRRLARRSLPQTEGELRLDCLDAPVEVVRDRHGIPHVYA
                     GSRLDLVRAQGYVHAQDRLFQMDTLRRYAFGRLAELAGRRALELDRTARRLLLRQAAE
                     RDAAACDDGTRALVEGYCEGVNAFLAHGPLPLELRLLRVRPEPWTPLDVQAPAAVLAL
                     SLSGNWTLELLRARLGVERLARLAPAYPAGHPVHLDDALVAEGLARAPATEGASNAFA
                     LAGSRTASGRPLVANDPHLLLGIPSVWHVQHLEWDGGMCAGVTVPGAPAVVLGRNRRI
                     AWGMTTAMVDTQDLFAVSLEDEAHEVVREPIRVRGRRAPVVEDVLVTGHGPVVARHEG
                     RAL"
BASE COUNT          651 a         1856 c         1825 g          605 t
ORIGIN      
        1 gtacgtctgc accgcctcct gcgcgacgta cgcgagcacg gcgtacgcgc cgccgaacga
       61 gacgagcgcg acgacggaga agaacgtccc ctcctgcacg tagacgctct cgcgccccag
      121 ccacgcggcg agcgcgagga ccggccccca ccagagcgcg agcccgagcg ccagcacgcc
      181 ggccccgcgg cggagcgagg ggcgcacgcg cggctcctcg tcgtcgtcgc gcccgtcctc
      241 gtccgcgagc gcgccggtga acgcgtccgg ccgccgccgt ccgccgagcc agcccacgac
      301 cgccgcgccg gccacgatga gcgggaacgg cacgtcgaag aagaagatcg ccacgaaggc
      361 cgcggccgca agtccgacga gcacgcggtt cccgagcacc cgcgacccga tgcgcacgac
      421 cgcgtacgcg acgatcgcga gcaccgccgg cttcaccccg tagaagaccg cctcgacgag
      481 cgccgtgtcg ccgtgccccg cgtagatcag gctgagcgcg aggatgagcg cgaagccggg
      541 gagcacgaag aggcttccgg cgatcaggcc gcctcgcacg ccgtgcagga gccagccgaa
      601 gtacgtcgcg agctgctgcg cctccggccc ggggagcagc atgcagtagt tgagcgcgtg
      661 gaggaagcgc cgctcgccga accagcgccg ctcctcgacg agcatccggt gcatgacggc
      721 gatctggccg gccggcccgc cgaagctgtt cagcccgacg agggcccaga cgcgcgccgc
      781 ctcgccgagg ccgatcccgt gccgacggtc catcccgcgg gagtatgggt agcgtcgccc
      841 cgtggcgcga gcgccgagcg aggagcccga ggggctcgtc taccgaccca acgtcctgag
      901 cgccgaggag gagcgggcgc tcctcggcgt gctcgccggc ctgcggttcg acccgatcgt
      961 catccgcggg caggcggcga agcgcacggc gcgccactac gggctcgact acgactacga
     1021 gtcgcgcgcg ccgaggcccg gggagccggt gccggactgg ctgctccccg cgcgcgcccg
     1081 ggcggccggg ctcgcgggcg tcgaggcgga ggagctcgtc gagatcctcg tccagcgcta
     1141 tccgcccggc tcgacgatcg gctggcaccg cgacgcgccc gcgttcgacc tcgtcgtcgg
     1201 tctctccctc ggcgcgccgg cccgcctacg cctccagcgc ggcaagggcg agcggcggcg
     1261 cgtgtgggag ctcgtcgccg agccccgctc cggctacgtg ctcgccggcg aggcccgccg
     1321 gtcgtgggag cactcgatcc cgccgacgaa agagctccgc cactcgatca cgttccgcac
     1381 gctgcggcgg cgttaaccgt cccttaaccg tcggaaacgg cggcctcgaa ggggccgggg
     1441 ctataacgaa tgccgtgaag ccggtgccgt tcgtcgccgc cctgctcgcc tccgggctcg
     1501 tcggaggagg cgtcgccttt gcgatcggca gcaccgtctg ggacggcgag acgacgaccg
     1561 tgatccgcga gagcgcgccc gccgatctgc aggcagccgc cttcgagtcg gacggcgccg
     1621 ccagcacggt cggcgaggtc tacgcgcgcg cggcgccggg cgtcgtgcag gtcacgtcga
     1681 gccgtgtcac gcagggcttc ttcggcccgc agcccggcga ggcgctcgga tccggcttcg
     1741 tgatcgacaa ggacggccac atcgtcacga actaccacgt catcgagggt gcggacgaga
     1801 tcctcgtgaa cttctcccag gacgacggcg tcgcggccga gctcgtcggc gtcgacccgt
     1861 ccaccgatct cgcgctcctc aagatccagg agcaccggcg cgcgctcacg ccgatcccgc
     1921 tcggcgactc ctccaccgtg cgcgtgggcg accaggtgat cgcgatcggc aacccgttcg
     1981 gcctcgaccg gacggtcacg gcggggatcg tgagcgcgct ccagcgccag atcctctcgc
     2041 cgagcaactt cacgatcgac aaggtgatcc agaccgacgc ggcgatcaac caggggaact
     2101 ccggcgggcc gctcctgaac gccgacggcg acgtgatcgg cgtgaacacg cagatcgcga
     2161 ccgcgggctc cgccgagggc aacgtcggga tcggcttcgc cgtcccggtg aacacggtga
     2221 agacggtcgt cggcgagctg ctcgagagcg ggcgcgtcga gcactcctat ctcggcgtgt
     2281 ccatgcagga cctgaccccg gacgtcgcgc gctacgtgaa cgtcccggag cgcggcgccc
     2341 tcgtcgcgca ggtctacccg ggcagcccgg ccgaggaggc ggggctccag ggcgctcagc
     2401 agacggtcgt gatcgacggc cagtcgtacg cggtcggcgg cgacgtcatc gtgcgcgcgg
     2461 acgggcggac gatccggacg tccgacgacc tccgccagat cgtcgccgcg aagaagccgg
     2521 gcgacgagct cgacctcggg atcctccgcg gcgaggagga ggtcgaggtc accgtgacgc
     2581 tcggcacgct gccggacgtc tcggccgaca cccgcggcta gccgccgccc ctagactccc
     2641 gcgcgtgatt ccgcacgcct tcggggagcg ggacggcctg acgctcggaa tcgaggagga
     2701 gctcttcgtc gtcgacgcgg agtcgctcga gccggcgccc gtgccgcccg aggtgctcga
     2761 cggggagcgc tgcaagccgg agctcttccg ggcggtcgtc gagctcacga cgggcgtctg
     2821 cgcgtcggtc gacgaggcgg cggccgagct cgaggagctc cgctccggcg cccgccgccg
     2881 cgcggccgac gccggcctcg cgctcgccgc gaccgggacg tggccgaccg cggtcgcgca
     2941 ggagcaggag atcacgcccg acccgggcta cctcgagttc gtcgagtacg ccggctcgtc
     3001 ggcgcggcgc cagttctgct ccgggctgca cgtccacgtc ggggtcgcga gcccggaggc
     3061 gtgcatggcc gcgctcgagc gggtgctccc gtggctcccg gtcgtgctgg cggcgtccgc
     3121 gaactcgccg tacctcgcgg gcgcggagac gggcctcgcg tcgacccgcg ccgagatcct
     3181 cgcgctcctc ccgcgaagcg ccggcccgcc cgtcttcggc tcgtacgagg agtgggagcg
     3241 cttcgccgag cggctcgtcg agctcggcct cgccgaccgt tacacccgga tctggtggga
     3301 catccgcccg cacccgacct tcgggacgct cgaggtgcgc acgcccgatc agccgacgcg
     3361 gctcgaggtg acggtcgcgt tcgcggcgct cgtgcaggcg ctcgtcgcga ccgcgaacgg
     3421 cacgccaccg gaggcgcccg ccgaccgcgg cctctacgcg cagaaccgct gggccgcggc
     3481 gcggttcggc gcgcaggccg agctcgtcca cccggacggg agccacgtcg tgcgcgcgga
     3541 cgagctcctc gccgagctgc tggagcgcgt cgcgcccgcc gcggagcggc tcggcacggc
     3601 tgtgtacctc gcaccgctcg aggggctcgt gcaggccgac acgcagctcg agcacggccg
     3661 tcgcgagggg ctccggtcgc tctgcgacgg cctcgtcgcc gccacgtagg atcagcccgt
     3721 ggccgtgcag tccgacacga tccaggtgac cggcatccgc tgcgagcggt gcgtcatgcg
     3781 gctcggcaag gcgctcgaag ggctcgaggg cctcgagggt gcgaacgcga cgctcatggg
     3841 gcaggtctcg ctcacatggg acgacgagcg ggtcgagcgc caggcgatcg tcgagcggat
     3901 gtcgcgcgcg ggcttccggc cggtggaatg acgagcgcgg cgctccggcg gctcgcccgc
     3961 cggtcgctgc cgcagacgga gggggagctc cggctcgact gcctcgacgc accggtcgag
     4021 gtcgtgcgcg accggcacgg gatcccgcac gtctacgccg ggagccggct cgacctcgtg
     4081 cgggcccagg gctacgtcca cgcgcaggac aggctcttcc agatggacac gctccgccgc
     4141 tacgcgttcg ggcggctcgc ggagctcgcc ggccgccggg cgctcgagct cgaccggacg
     4201 gcgcgccggc tgctcctgcg gcaggccgcc gagcgcgacg ccgccgcgtg cgacgatggg
     4261 acgcgcgcgc tcgtcgaggg ctactgcgag ggcgtgaacg cgttcctcgc gcacgggccg
     4321 ctcccgctcg agctgcggct gctccgcgtc cggcccgagc cgtggacgcc gctcgacgtg
     4381 caggcgcccg cggccgtgct cgcgctctcg ctctccggga actggacgct cgagctcctc
     4441 cgggcacggc tcggggtgga gcggctcgca cggctggccc ccgcctaccc cgcggggcat
     4501 ccggtgcacc tggacgacgc cctcgtcgcc gaggggctcg cccgcgcgcc ggcgacggag
     4561 ggcgcctcga acgccttcgc cctcgccggc tcgcgcacgg cgtccggccg tccgctcgtc
     4621 gcgaacgacc cgcacctcct gctcgggatc ccgtccgtct ggcacgtcca gcacctcgag
     4681 tgggacggcg gcatgtgcgc gggcgtcacg gtgccgggcg cgcccgcggt cgtgctgggg
     4741 cggaaccggc ggatcgcgtg ggggatgacg accgcgatgg tcgacacgca ggatctcttc
     4801 gcggtctcgc tcgaggacga ggcgcacgag gtcgtgcgcg agccgatccg ggtgcgcggt
     4861 cgtcgggcgc cggtcgtcga ggacgtcctc gtcaccggcc acgggccggt cgtcgcgcgc
     4921 cacgagggcc gggcgct
//
LOCUS       DASTFC010000326         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_717854,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000326 DASTFC010000000
VERSION     DASTFC010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717854"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..892)
                     /locus_tag="VFO88_09615"
     CDS             complement(332..892)
                     /locus_tag="VFO88_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9545824.1"
                     /translation="MTLVIVLVLVAVVLGLGLILILLYNKLVTLRNRAENAWSQVDVQ
                     LRRRYDLIPNLVETVKGYASHERATFEEVTNARTRAQEARTVGEQAEAENLLTQAIGK
                     LFAVAEDYPELRATENFQQLQAQLDETENKIAVSRQVYNDAVLTYDTALETVPTNIVA
                     GMFNFEERQYFELEEPAAREAPQVRF"
     gene            complement(924..1637)
                     /locus_tag="VFO88_09620"
     CDS             complement(924..1637)
                     /locus_tag="VFO88_09620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9545825.1"
                     /translation="MALPPSSYDAIAELYDDWSRSVVEDVGFYVDEALAAGGPVVELG
                     VGTGRIAVPVALAGVRVIGVDSSPGMLEVCRRHAEAAGVADFVDLRLGDLRRPPVAER
                     VGLVLAPFRSFLHMETDEARLEALRATRALLVPGGRFVFDVFAPGADDIAETHGRWIE
                     REPGIFERAEWDEERSRLVLGVRGPGGETTMSLAWLPPERWTALVEEAGFEVLATYGW
                     FDRKPYAGGEDTIWVARRP"
     gene            1661..>2217
                     /locus_tag="VFO88_09625"
     CDS             1661..>2217
                     /locus_tag="VFO88_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9545826.1"
                     /translation="MDAQELETEAASAVARASTLSELDEARVRFLGRKSPLKQALREV
                     RDRESGMALNAVREAIEAAVAARQEELERAELERRLAEEVVDVTLPGEATPRGHLHLI
                     TQIRREVEDVFLGLGYRVVDGREVETTHYNFDALNFPHWHPARSRLASLFLNGNVLLR
                     TETSPSQIRVMEESPPPIYMISLGRV"
BASE COUNT          316 a          829 c          777 g          295 t
ORIGIN      
        1 ccgtcgatcg ggacgtcgcc gaacgtgccc ggctcgccgc tcgagccgag ctccgtcgga
       61 gcgcccggcc ggtaccgctg gtcgccctcg gcgacgaaga cgtccgagat cgactcgccc
      121 tcgcggagcg ggatgtcccg gtacccgccg gtgaagacgc cgttgaacga gaacgtgatc
      181 cgctcctcga tcgcgagcgt cccgtccggc tcgaccgtga cgtggacgtc cgcgtccggg
      241 agcgagaacg accgctcctg cgccgcggca gggccggcga ggacgagcga gaccgccgcg
      301 gcggccgcga cggcgacgag ccgccgtacg ctcagaagcg gacctgcggc gcctcgcgcg
      361 ccgccggctc ctcgagctcg aagtactgcc gttcctcgaa gttgaacatc cccgcgacga
      421 tgttcgtcgg caccgtctcg agcgccgtgt cgtacgtgag cacggcgtcg ttgtagacct
      481 ggcgggagac ggcgatcttg ttctccgtct cgtcgagctg cgcctggagc tgctggaagt
      541 tctccgtcgc gcgcagctcg gggtagtcct ccgcgaccgc gaagagcttc ccgatcgcct
      601 gcgtgaggag gttctccgcc tcggcctgct cgccgacggt gcgggcctcc tgcgcccggg
      661 tgcgcgcgtt cgtcacctcc tcgaacgtcg cccgctcatg gctcgcgtag cccttgaccg
      721 tctcgacgag gttcggaatg aggtcgtagc ggcgtcggag ctgcacgtcg acctgcgacc
      781 acgcgttctc cgcgcggttc cgcagggtga cgagcttgtt gtagaggagg atcaggatca
      841 gcccgaggcc gagcacgacg gcgacgagca cgagcacgat cacgagggtc acgcgcccac
      901 cctaaaccgg tctgcggccg tcgtcacggg cgccgggcga cccagatcgt gtcctcgccg
      961 cccgcgtacg gcttgcggtc gaaccagccg tacgtcgcga ggacctcgaa cccggcctcc
     1021 tcgacgaggg ccgtccagcg ctcgggcggg agccatgcga ggctcatcgt cgtctcgccg
     1081 cccggcccgc gcacgccgag cacgaggcgc gagcgctcct cgtcccactc ggcgcgctcg
     1141 aagatgcccg gctcgcgctc gatccagcgc ccgtgcgtct cggcgatgtc gtccgcgccg
     1201 ggcgcgaaga cgtcgaagac gaagcgcccg cccggcacga gcagcgcgcg cgtcgcgcga
     1261 agcgcctcga gccgcgcctc gtccgtctcc atgtggagga acgagcgaaa cggcgccagg
     1321 acgagcccga cgcgctcggc gaccggcggc cggcgcaggt cgccgaggcg gaggtcgacg
     1381 aagtcggcca ccccggcggc ctccgcgtgc cggcggcaga cctcgagcat cccgggcgag
     1441 gagtcgaccc cgatcacgcg cacgccggcg agcgcgaccg gcacggcgat ccgccccgtg
     1501 ccgacgccga gctccacgac ggggccgccg gccgcgagcg cctcgtcgac gtagaagccc
     1561 acgtcctcga cgacgctccg gctccagtcg tcgtagagct ccgcgatcgc gtcgtaggag
     1621 ctcggaggga gggccacggg cccgatgata cgctcagccg gtggacgcgc aggagctcga
     1681 aaccgaggcg gcaagcgccg ttgcgcgcgc ctcgaccctt tccgagctcg acgaggcgcg
     1741 cgtccggttc ctcgggcgga agagccccct gaagcaggcg ctccgcgagg tccgcgaccg
     1801 cgagtcgggc atggcgctga acgccgtccg cgaggcgatc gaggccgccg tcgccgcccg
     1861 ccaggaggag ctcgagcgcg ccgagctcga gcgccggctc gcggaggagg tcgtcgacgt
     1921 gacgctcccc ggcgaggcga cgccgcgggg ccatctccac ctgatcacgc agatccggcg
     1981 cgaggtcgag gacgtcttcc tcggcctcgg ctaccgcgtc gtcgacggcc gggaggtcga
     2041 gacgacgcac tacaacttcg acgcgctcaa cttcccccac tggcacccgg cgcgctcacg
     2101 cctcgcgtcg ctcttcctga acggcaacgt cctcctgcgc accgagacct cgccctcgca
     2161 gatccgcgtg atggaggagt cgccgccgcc gatctacatg atctcgctcg gccgggt
//
LOCUS       DASTFC010000327         3580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_404586,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000327 DASTFC010000000
VERSION     DASTFC010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404586"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..633
                     /locus_tag="VFO88_09630"
     CDS             109..633
                     /locus_tag="VFO88_09630"
                     /inference="COORDINATES: protein motif:HMM:NF022798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3156 family protein"
                     /protein_id="HET9545827.1"
                     /translation="MRGRARAAAARSLEFDLDTFEAVGYRRGGKAGPLAVRLEPPEGL
                     PPLVLRLAPEGRIFGGAFALEVSTTEPVLPSTQGLSARGRGVVRLARIAWRARRGDAA
                     GEALARRLDEDAALQEKLAAVHFERLRVEPDGRPVVRHMGGSVVWILFPPVVRPIPLT
                     PEQARATARALGAF"
     gene            complement(630..1085)
                     /locus_tag="VFO88_09635"
     CDS             complement(630..1085)
                     /locus_tag="VFO88_09635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9545828.1"
                     /translation="MLRTDHYPFCWGCSVGTDGLGFDWRFEGDTLHAEHVVGEEFQGA
                     PGMAHGGIVAALLDEACGQAARPLVSPGVTSRLEVRYLAPVPVEEPLRISAQVVERGD
                     RRVRAEASILDRDGLVLAHAQADVVLVRPEHFLSTERGRARGLDWLPRD"
     gene            complement(1119..1544)
                     /locus_tag="VFO88_09640"
     CDS             complement(1119..1544)
                     /locus_tag="VFO88_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545829.1"
                     /translation="MKDRVNMAVDTARPYVERLAKDDELHDHVKKAYDSARKIYDDLI
                     GPTGASAVALKVARDKDVQDELRKAVEELREAGKRARGDESHTGRNMTLLLAGIALGV
                     LFNPMTGPETRAWIKEKVFGPDEPFEFEPSSSGSSSNES"
     gene            1635..2378
                     /locus_tag="VFO88_09645"
     CDS             1635..2378
                     /locus_tag="VFO88_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9545830.1"
                     /translation="MKGVILAGGSGTRLHPLTRITNKHLLPIYDRPMVTYSIECLVRA
                     GITELMVVTGGTHAGEFLRLLGNGHEYGIERLSYAYQERAGGIAEALGLAERFAAGER
                     VCVMLADNVVERSIRSSAENFERQERGARILLAREEDPEHLRHLGVPELDGDGRVLGI
                     VEKPETPPSPFAVTGIYFYDADVFEIIPGLVPSGRGELEITDVNNHYVEQGTMEYDVL
                     EGFWGDAGESIDAYYAVNDFVRANGANSE"
     gene            2375..2851
                     /locus_tag="VFO88_09650"
     CDS             2375..2851
                     /locus_tag="VFO88_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET9545831.1"
                     /translation="MSAPAATLPGLLRLPLRVFEDERGWFCEVRRASALPRPTVQTNV
                     SFSRRGVIRGLHYHERGQDDLFTCLTGTARVVVLDRETGDVFTEDIGEENPVAIYVPG
                     HFAHGFEALTDLLFCYHVTEEYDPADPDEHGVPWDDPRVRDLWSTQTPILSARDAS"
     gene            2806..>3580
                     /gene="rfbD"
                     /locus_tag="VFO88_09655"
     CDS             2806..>3580
                     /gene="rfbD"
                     /locus_tag="VFO88_09655"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET9545832.1"
                     /translation="MEHADADPLGSRRVLITGGAGQLGVALAEAFPGADVRPRASLDV
                     TQPFSAEADLVLHAAAWTNVDGAESDPEAAELVNVVGTRNVVALGAPVVYYSSDYVFD
                     GEKRSPYVESDAPAPRSVYGRTKLDGEREVRDGWIVRTSWLFGWTGKNFVRTMLRLGA
                     ERDEVRVVDDQRGSPTYVGHLAEATRSLVELPGGVWHVAAEGDCTWAELAAAIFEEAG
                     LACRVVPIMTEEFGAPAPRPAYSVLRSERPGAPRLPHWRE"
BASE COUNT          470 a         1285 c         1320 g          505 t
ORIGIN      
        1 agctgctccc gcgactcgac gcgctcgagg tggcgggccc ggtcgagcgc atgcggacga
       61 acttcacgaa cgcgatcaag cggatgcccg tccgcgtcac ggcgcgcggt gcgcggtagg
      121 gcgcgcgcgg ccgccgcccg ctcgctcgag ttcgacctcg acaccttcga ggcggtcggc
      181 taccggcgcg gcgggaaggc agggccgctc gccgtgcgcc tcgagccgcc ggaggggctg
      241 ccgccgctcg tcctccggct cgcgcccgag ggtcggatct tcggcggggc gttcgccctc
      301 gaggtctcga ccacggagcc ggtgctgccg tcgacgcagg gcctctcggc gcgcgggcga
      361 ggcgtcgtcc ggctcgcccg gatcgcatgg cgcgcgcggc gcggcgacgc ggcgggcgag
      421 gcgctcgccc ggcggctcga cgaggacgcg gcgctccagg agaagctcgc cgccgtgcac
      481 ttcgagcggc tgcgcgtcga gcccgacggg cgccccgtcg tccgccacat gggcggctcc
      541 gtcgtctgga tcctcttccc cccggtcgtg cgcccgatcc cgctcacgcc cgagcaggcg
      601 cgcgcgaccg cccgcgcgct cggcgccttc tagtcgcgcg gcaaccagtc gaggccgcgc
      661 gcccggccgc gctcggtgga gaggaagtgc tccggccgga cgaggacgac gtcggcctgc
      721 gcgtgcgcga ggacgagccc gtcgcggtcg aggatcgagg cttccgcgcg cacccggcgg
      781 tcgccccgct cgacgacctg cgccgagatc cggaggggct cctccaccgg cacgggcgcg
      841 aggtagcgca cctcgaggcg cgacgtcacc cccggcgaca cgagcggccg cgcggcctgg
      901 ccgcacgcct cgtcgaggag cgcggcgacg atcccgccgt gcgccatccc gggcgcgccc
      961 tggaactcct cgccgacgac gtgctccgcg tggagcgtgt cgccctcgaa gcgccagtcg
     1021 aacccgagcc cgtccgtgcc gaccgagcag ccccagcaga acgggtagtg gtcggtccgg
     1081 agcacgccgg gccgattatg aagccgtcgt cggcggcgtt acgactcgtt gctgctcgag
     1141 cccgagctcg acggctcgaa ctcgaacggc tcgtccgggc cgaacacctt ctccttgatc
     1201 cacgcgcgcg tctcgggccc ggtcatcggg ttgaagagaa cgccgagcgc gatccccgcg
     1261 aggagcaggg tcatgttgcg gccggtgtgc gactcgtcgc cgcgcgcccg cttgcccgcc
     1321 tcgcggagct cctcgaccgc cttgcggagc tcgtcctgga cgtccttgtc cctggcgacc
     1381 ttgagggcca ccgcggaggc gcccgtcggg ccgatcaggt cgtcgtagat cttccgcgcc
     1441 gagtcgtacg ccttcttcac gtggtcgtgg agctcgtcgt ccttcgcgag ccgctcgacg
     1501 tacggccggg cggtgtccac tgccatgttc accctgtcct tcatcgcatc ctttcgtcgg
     1561 tgtggaggga ttcttacctg agcgagcgtg accaaacagc ggagcaaccc ctccgtcgtt
     1621 agggtttccg ccggatgaag ggcgtcattc tcgccggcgg aagcggcacg cgcctgcacc
     1681 cgctcacacg gatcacgaac aagcacctgc tgccgatcta cgaccggccg atggtcacgt
     1741 actcgatcga gtgcctcgtg cgcgcgggga tcaccgagct catggtggtg acgggcggca
     1801 cgcacgcggg cgagttcctg cgcctgctcg gcaacgggca cgagtacggg atcgagcggc
     1861 tctcgtacgc ctaccaggag cgcgcgggcg ggatcgccga ggcgctcggg ctcgcggagc
     1921 gcttcgcggc cggcgagcgg gtgtgcgtga tgctcgcgga caacgtggtc gagcgctcga
     1981 ttcgctcgtc ggccgagaac ttcgagcgcc aggagcgtgg cgcccggatc ctgctcgccc
     2041 gcgaggagga tcccgagcac ctgcggcatc tcggcgtgcc ggagctcgac ggcgacgggc
     2101 gcgtcctggg gatcgtcgag aagccggaga ccccgccgag cccgttcgcg gtgaccggga
     2161 tctacttcta cgacgcggac gtcttcgaga tcatcccggg cctcgtcccg tcgggccgcg
     2221 gcgagctcga gatcacggac gtgaacaacc actacgtcga gcaggggacg atggagtacg
     2281 acgtcctcga ggggttctgg ggcgacgcgg gcgagtcgat cgacgcgtac tacgccgtga
     2341 acgacttcgt gcgcgccaac ggggcgaaca gcgagtgagc gcgcccgcgg cgacactgcc
     2401 ggggctgctc cgcctgccgc tgcgggtctt cgaggacgag cgcggctggt tctgcgaggt
     2461 gcggcgcgcg tcggcgctgc cgcggccgac ggtgcagacg aacgtctcgt tctcccgccg
     2521 gggcgtgatc cgcggcctgc actaccacga gcgcgggcag gacgacctct tcacgtgcct
     2581 gaccgggacg gcccgcgtag tcgtcctcga ccgcgagacg ggcgacgtct tcaccgagga
     2641 catcggcgag gagaaccccg tcgcgatcta cgtcccaggg cacttcgcgc acggattcga
     2701 ggcgctcacc gatctcctct tctgctacca cgtgaccgag gagtacgacc ccgccgaccc
     2761 cgacgagcac ggcgtcccct gggacgaccc gcgcgtgagg gacctgtgga gcacgcagac
     2821 gccgatcctc tcggctcgcg acgcgtcctg atcaccggag gggcggggca gctcggggtc
     2881 gcgctggccg aggcgttccc cggtgcggac gtccggcctc gggcctcgct cgacgtcacg
     2941 cagccgttct cggcggaggc ggatctcgtc ctgcacgcgg ccgcgtggac gaacgtggac
     3001 ggcgcggaga gcgacccgga ggccgccgag ctcgtgaacg tcgtcggtac gcggaacgtc
     3061 gtcgcgctcg gcgcgccggt cgtctactac tcgagcgact acgtcttcga cggggaaaag
     3121 cgctcgccgt acgtcgagtc ggacgcgccc gcgccgcggt cggtgtacgg gcggacgaag
     3181 ctcgacggcg agcgggaggt gcgggacggc tggatcgtgc ggacgtcctg gctcttcggc
     3241 tggacgggga agaacttcgt ccgcacgatg ctccggctcg gagcggagcg ggacgaggtg
     3301 cgcgtcgtgg acgaccagcg cggctcgccg acgtacgtcg ggcacctcgc cgaggcaacg
     3361 cgctccctcg tagagcttcc cggcggcgtc tggcacgtcg ccgcagaggg ggactgcacg
     3421 tgggcggagc tcgcggccgc gatcttcgag gaggcggggc tcgcgtgccg tgtcgtcccg
     3481 atcatgacgg aggagttcgg cgcgcccgcc ccgcgcccgg cgtactcggt cctgcggagc
     3541 gagcgccccg gcgcgccgcg gctcccgcac tggcgcgaac
//
LOCUS       DASTFC010000328         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1064356,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000328 DASTFC010000000
VERSION     DASTFC010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064356"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VFO88_09660"
     CDS             <1..1166
                     /locus_tag="VFO88_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET9545833.1"
                     /translation="EPGRLADVEAVCRRWELEHAVIGEVTETGELRAFFGGELVGAIP
                     ARLLTDEAPRYRVSPEPRALAAPIVHDPIPVEPDDLLALLASPNVRSRRAVFERYDHL
                     VGSRTVRRPGLDAAVLRLPPSHRGLAVSLDGPGRAAPLDPHTAGMQAVLESARNVACA
                     GGRPLALTNCLNFGNPEKGEVAWELVEAIEGMAQACEALGVPIVSGNVSLYNETGGRP
                     IDPTPAVGCVGLVEDVREIPGRWAEGDVVLVAGAPELSLDGSEYQARFLGGAAGRPPR
                     PSVAAEAELVAFLAEAAPVLTSAHDCSDGGLAVALAELALWSGAGADVDLEPDALEWF
                     GEGGGRAVVTCRREDVTRLGDAVALREIGVVRGDRLLGVTLDRLRAAHEGGER"
     gene            1166..>2178
                     /gene="purF"
                     /locus_tag="VFO88_09665"
     CDS             1166..>2178
                     /gene="purF"
                     /locus_tag="VFO88_09665"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET9545834.1"
                     /translation="MCGVLGIRAPERDVARLAYFGLFALQHRGQESAGIAVSDHGRLT
                     ALRDMGLVTQVFSEDKLQGLRGEAAIAHCRYSTTGSVAWQNAQPLVQHGAARTVALGH
                     NGNLLNTTELRAELAEDGVKLRTTSDTEVIAALIAHDERPLEEAVAAAMRRLRGAYTI
                     VLLAEGKLIGFRDPDGIRPLVIGLLGEDYVLASESCALDLLGAELVREVAPGELVVVD
                     EDGLRAAQGVPAHEGGALCIFEFIYFGRPDSKLRGVELHGARVRMGERLAAEAPVEAD
                     MVVPIPDSGTPAAIGFARASGIPFNEGLIKNRYVGRTFIQPDQALREHGIKTKFNPLA
                     EVSG"
BASE COUNT          254 a          804 c          835 g          285 t
ORIGIN      
        1 tcgagcccgg gcggctcgcc gacgtcgagg cggtctgccg ccggtgggag ctcgagcacg
       61 cggtgatcgg cgaggtgacg gagacgggcg agctgcgcgc gttcttcggc ggcgagctcg
      121 tcggcgcgat cccggcgcgg ctcctcaccg acgaggcgcc gcgctaccgg gtctcgcccg
      181 agccgcgggc gctcgccgcg ccgatcgtcc acgacccgat ccccgtcgag ccggacgacc
      241 tgctcgcgct gctcgcctcc cccaacgtcc gctcgcgccg ggccgtgttc gagcgctacg
      301 accatcttgt gggctcgcgc acggttcgcc ggcccgggct cgacgccgcg gtgcttcgcc
      361 tccccccctc gcaccgcggg ctcgccgtct cgctcgacgg cccggggcgc gcggcgcccc
      421 tcgacccgca cacggcgggg atgcaggcgg tcctcgagtc cgcgcgcaac gtcgcctgcg
      481 ccggcggccg gccgctcgcg ctcacgaact gcctcaactt cgggaacccg gagaagggcg
      541 aggtcgcctg ggagctcgtc gaggcgatcg agggcatggc gcaggcgtgc gaggcgctcg
      601 gcgtcccgat cgtctccgga aatgtgtcgc tctacaacga gacgggcggg cggccgattg
      661 acccgacccc cgccgtgggg tgcgtcgggc tcgtcgagga cgtgcgcgag atcccgggcc
      721 ggtgggcgga gggggacgtc gtcctcgtcg cgggcgcgcc ggagctctcg ctcgacggct
      781 ccgagtacca ggcgcgcttc ctcggcggcg cggccggccg gccgccgcgg ccgagcgtcg
      841 cggccgaggc cgagctcgtc gccttcctcg cggaagcggc gcccgtgctg acgagcgcgc
      901 acgactgctc ggacggcggt cttgcggtcg cgctcgcgga gctcgcgctc tggtcgggcg
      961 ccggagcgga cgtcgacctc gagccggacg cgctcgagtg gttcggcgag ggcggcgggc
     1021 gcgcggtcgt gacgtgccgg cgcgaggacg ttacgcgcct cggcgatgcg gtcgcgctgc
     1081 gcgagatcgg cgtcgtccgc ggcgaccggc tcctcggcgt gacgctcgac cgcctgcgcg
     1141 cggcgcacga gggaggggag cgctgatgtg cggcgtcctc ggcatccgcg cgcccgagcg
     1201 cgacgtcgcg cggctcgcgt acttcgggct cttcgcgctc cagcaccgcg gccaggagtc
     1261 ggccggcatc gccgtctccg accacggccg cctcacggcc ctgcgggaca tggggctcgt
     1321 gacgcaggtg ttctccgagg acaagctcca ggggctccgc ggcgaggcgg cgatcgcgca
     1381 ctgccgctac tcgaccaccg gctcggtcgc ctggcagaac gcgcagccgc tcgtccagca
     1441 cggggcggcg cgcacggtcg cgctcgggca caacggcaac ctcctgaaca cgacggagct
     1501 gcgcgccgag ctcgccgagg acggcgtgaa gctccgcacg acctccgaca ccgaggtgat
     1561 cgcggcgctc atcgcgcacg acgagcggcc gctcgaggag gcggtcgccg ccgcgatgcg
     1621 ccgtctgcgc ggggcgtaca cgatcgtgct cctcgccgag ggaaagctga tcggcttccg
     1681 cgacccggac gggatccggc cgctcgtgat cggcctgctc ggcgaggact acgtgctcgc
     1741 gtccgagtcg tgcgcgctcg atctactcgg cgccgagctc gtccgcgagg tggcgccggg
     1801 cgagctcgtc gtcgtggacg aggacggcct ccgcgccgcg cagggcgtcc cggcgcacga
     1861 gggcggcgcg ctctgcatct tcgagttcat ctacttcggg cggccggact cgaagctccg
     1921 cggggtcgag ctgcacggcg cgcgggtgcg gatgggggag cggctggccg ccgaggcgcc
     1981 cgtcgaggcg gacatggtgg tcccgatccc ggactccggc acgccggccg cgatcggctt
     2041 cgcgcgcgcg agcgggatcc ccttcaacga gggcctgatc aagaaccgct acgtcgggcg
     2101 gacgttcatc cagcccgacc aggcgctgcg cgagcacggg atcaagacga agttcaaccc
     2161 gctcgcagag gtctcggg
//
LOCUS       DASTFC010000329         3424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1624249,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000329 DASTFC010000000
VERSION     DASTFC010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624249"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFO88_09670"
     CDS             <1..740
                     /locus_tag="VFO88_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545835.1"
                     /translation="LLPGIVTPAAVAYERLVPALGPEVDARPKELEVYAGTEPPPGSS
                     LEVEMDGVLRAADDAGWERFHLVGYSGGGAVAASLAASRPERLLSLGLLEPAWTGNGG
                     LSETEAKARAEFDALDGLEPREFLATFARLQLRPGVEPPPPPDPPPPWMATRPAAIAA
                     LTLAFRTGELSAESLRRFGRPVYHALGGRSRPELYGEQSARLARLFPAFTEEVFEERH
                     HFDPPHRAEPERLAASLLAHWRRAESG"
     gene            complement(737..1309)
                     /locus_tag="VFO88_09675"
     CDS             complement(737..1309)
                     /locus_tag="VFO88_09675"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HET9545836.1"
                     /translation="MSLYEILLFGHITTAILWVGAGVSLVVVALLAERKDDDGGLRTS
                     LDVANRLGNVYFLPISALVIVFGIGLVAESDAWSFSQLWIVLGLVGYALTFLTGLLVI
                     KPRAERIGKLIEAARGEMTPEALFEGRKLLTLARIDYIVLLLVVLDMVVKPTGDDVAL
                     LVVMAAILVAGVAYVLWRARSLTPPAAATT"
     gene            complement(1340..2302)
                     /locus_tag="VFO88_09680"
     CDS             complement(1340..2302)
                     /locus_tag="VFO88_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET9545837.1"
                     /translation="MSTAALPDSVAARANVEPFVHRTPLLTSRSLDDETGLEVRLKAE
                     LLQRTGAYKIRGALNKLSLLSDEERARGVVCSSAGNHAQGVALAAQMLGIRAVVCMAA
                     NATPSKVAATRGYGAEVVQHGEIWDEADEKARELVERDGLVYVHPFDDLDLIAGQGTL
                     GLEVVEDWPEVEAVVVPIGGGGLISGVAAAVKRARPEVRVVGVESSDGPAMKASLDAG
                     ELVTLERCDTIIDGLRVKRVGRNTFELVRRFVDDVVTVPDDVIFANVVWTMGRLKLVV
                     EGAAAAPVAALRSGALALPPRTKVVLVLSGGNLDLSRLDGLRWN"
     gene            2398..>3424
                     /locus_tag="VFO88_09685"
     CDS             2398..>3424
                     /locus_tag="VFO88_09685"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HET9545838.1"
                     /translation="MGPAEFLDLPWEAPLEEWPSELLVQATRGLGRHVVRFVDRGGAL
                     FALKELQAPLAEREYRLLRRLAAEGVPAVEGVGVVRERETADGEPLADIVLTRYLEFS
                     LPLRVMIARRPLPELLDRLLDAFAGLLVRLHLAGFFWGDCSLSNTLFRRDAGALAAAL
                     VDAETCEHHARLTDGQRAYDLQIAEENIFGELLDLEAQAGETVTAAPDPADIVADVRA
                     RYERLWSDLTAEEVFARHERHRLEERLRRLNELGFDAEEVEIVSDEHGYRLHLEPGVV
                     EPGHHRRLLARLTGLSAQENQARRLLADIASFRRALEERGERFVSEPAAAGRWLAEVF
                     EPTVASIP"
BASE COUNT          455 a         1284 c         1245 g          440 t
ORIGIN      
        1 tgctcctgcc gggaatcgtc acgccggccg ccgtggccta cgagcggctc gtgcccgcgc
       61 tcgggccgga ggtcgacgcg cgcccgaagg agctcgaggt ctacgccggc acggagccgc
      121 cgccgggctc ctcgctcgag gtggagatgg acggcgtcct gcgcgcggcc gacgacgccg
      181 gctgggagcg gttccacctc gtcggctact cgggcggcgg ggcggtcgcg gcgtcgcttg
      241 cggcctcgcg cccggagcgg ctcctgagcc tcggcctcct cgagcccgcc tggacgggca
      301 acggcgggct gagcgagacg gaggcgaagg cgcgcgccga gttcgacgcg ctcgacggcc
      361 tcgagccgcg ggagttcctc gcgaccttcg cccgcctgca gctccggccc ggcgtcgagc
      421 cgccgccgcc gccggacccg ccgccgccgt ggatggcgac ccgccctgcc gcgatcgccg
      481 cgctgaccct cgcgttccgc acgggcgagc tgtccgcgga gtcgctgcgc cgcttcggcc
      541 ggcccgtcta ccacgcgctc ggcggccgct cccgccccga gctctacggc gagcagtcgg
      601 cccgcctcgc ccgcctcttc cccgccttca cggaggaggt cttcgaggag cgccaccact
      661 tcgacccgcc gcaccgtgcc gagccggagc ggctcgcggc ctcgcttctc gcccactggc
      721 gccgcgcgga gtcgggctag gttgtcgcag ccgcgggcgg cgtgagcgag cgcgcgcgcc
      781 agaggacgta ggcgacgccg gcgacgagga tcgccgccat cacgacgagc agcgcgacgt
      841 cgtcgccggt cggcttcacg accatgtcga ggacgacgag caagagcacg atgtagtcga
      901 tccgggcgag cgtgaggagc ttgcggccct cgaacagcgc ctcgggcgtc atctcgccgc
      961 gcgccgcctc gatgagcttc ccgatccgct ccgcgcgcgg cttgatcacg aggaggccgg
     1021 tgaggaacgt gagcgcgtac ccgacgagcc cgagcacgat ccagagctgc gagaacgacc
     1081 aggcgtcgct ctcggcgacg agcccgatcc cgaacacgat cacgagcgcc gagatcggga
     1141 ggaagtagac gttcccgagc cggttcgcga cgtcgagcga ggtgcgcagg ccgccgtcgt
     1201 cgtccttccg ctccgcgagc agcgcgacga cgacgagcga gacccccgcc ccgacccaga
     1261 ggatcgcggt cgtgatgtgc ccgaacagca ggatctcgta gagcgacacc ggcccggggt
     1321 ttctacacga acggccggac tagttccagc ggagaccgtc gagccgagac aggtcgaggt
     1381 tcccgccgga gaggacgagg acgaccttcg tccggggagg aagcgcgagc gcgcccgagc
     1441 ggagggcggc cacgggtgcg gcggccgcgc cctcgacgac gagcttcagg cggcccatcg
     1501 tccagacgac gttcgcgaag atgacgtcgt ccggaacggt cacgacgtcg tccacgaagc
     1561 ggcggacgag ctcgaacgtg tttcgcccga cccgcttcac gcgaaggccg tcgatgatcg
     1621 tgtcgcagcg ctcgagcgtc acgagctcgc ccgcgtcgag cgacgccttc atcgccgggc
     1681 cgtccgagga ctcgaccccg acgacccgca cctcgggccg cgcgcgcttg accgcggccg
     1741 cgacgccgga gatcagcccg ccgccgccga tcgggacgac gaccgcctcc acctcgggcc
     1801 agtcctcgac gacctccagg ccgagggtgc cctggccggc gatcaggtcg aggtcgtcga
     1861 acgggtggac gtagacgagc ccgtcccgct cgacgagctc gcgcgccttc tcgtccgctt
     1921 cgtcccagat ctcgccgtgc tggacgacct ccgcgccgta cccgcgcgtc gccgcgacct
     1981 tggacggggt cgcgttcgcg gccatgcaga cgaccgcgcg gatcccgagc atctgcgccg
     2041 cgagcgcgac gccctgcgcg tggttcccgg ccgacgagca gacgaccccg cgcgcccgtt
     2101 cctcgtccga gaggagcgag agcttgttca gcgcgccgcg gatcttgtac gcgcccgtgc
     2161 gctgaagcag ctcggccttc aggcgcacct cgagccccgt ctcgtcatcg agcgagcggg
     2221 acgtgaggag cggcgtccgg tgcacgaacg gctcgacgtt cgcccgcgcg gcgaccgaat
     2281 ccggaagcgc ggcggtgctc atacggcccg agtctgacgc atcgcggcga cgcggggtag
     2341 cgtgactccg ttcccgagcc cgaggaggcg cgcacgagga tccagatcgt cccgtcggtg
     2401 gggcccgccg agttcctcga cctgccctgg gaggcgccgc tcgaggaatg gccgtccgag
     2461 ctcctcgtgc aggcgacgcg cgggctcggc cgccacgtcg tgcggttcgt cgaccgcggg
     2521 ggcgcgctgt tcgcgctgaa ggagctccag gcgccgctcg ccgagcgcga gtaccggctg
     2581 ctgcgccgcc tcgcggccga gggcgtgccg gcggtcgagg gtgtcggcgt cgtccgcgag
     2641 cgcgagacgg cggacggcga gccgctcgcg gacatcgtcc tcacgcgcta cctcgagttc
     2701 tcgctccccc tgcgcgtcat gatcgcgcgc cggccgctcc cggagctgct cgaccggctg
     2761 ctcgacgcgt tcgccgggct gctcgtccgg ctccacctcg ccgggttctt ctggggagac
     2821 tgctcgctct cgaacacgct cttccggcgc gatgcgggcg cgctcgccgc ggcgctcgtg
     2881 gacgccgaga cgtgcgagca ccacgcacgg ctcaccgacg gacagcgcgc gtacgacctc
     2941 cagatcgcgg aggagaacat cttcggcgag ctcctcgacc tcgaggcgca ggcgggcgag
     3001 acggtcacgg cggcgcccga cccggcggac atcgtcgccg acgtccgggc ccgctacgag
     3061 cggctctgga gcgacctgac cgccgaggag gtcttcgcgc ggcacgagcg tcaccggctc
     3121 gaggagcggc tccggcggct gaacgagctg ggcttcgacg cggaggaggt cgagatcgtc
     3181 tcggacgagc acggctaccg gctccacctc gagccgggcg tcgtcgagcc gggccaccac
     3241 cggcggctgc tcgcgcgtct gaccggcctc tcggcgcagg agaaccaggc gcggcggctg
     3301 ctcgcggaca tcgcctcgtt ccgccgggcg ctcgaggagc gcggcgagcg gttcgtctcc
     3361 gagccggccg cggccgggcg ctggctcgcg gaggtcttcg agccgaccgt cgcctcgatc
     3421 cccg
//
LOCUS       DASTFC010000330        11376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1064418,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000330 DASTFC010000000
VERSION     DASTFC010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064418"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFO88_09690"
     CDS             <1..412
                     /locus_tag="VFO88_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545839.1"
                     /translation="PDALPANVTIAHNTILSGARRGFGQYPDLANSLVVSPRYGWNPE
                     SQRPLVVNNVIALTGTPDELCGLARTARNVTIRGEACAEEDVVGDPLLTDAGVPGPHS
                     TLVVDRGLPGFSAVDLLGRPRDEAPDVGAYEVMR"
     gene            472..1077
                     /locus_tag="VFO88_09695"
     CDS             472..1077
                     /locus_tag="VFO88_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545840.1"
                     /translation="MRGTGSFRRSATVIAALAGALVLPAGASASVETFERFEAVATAN
                     FTVTEQCADGSTVQTLVTVLGGHEVEVESGETTLDNDFLTVLVRGFDCSGAFVNDRGS
                     GPATFTFSPSLQTASVTGTITTRDGRTVSADVAWEGTGALEASSNTTTFPGFTGHFRS
                     TRRDAIATGTVVVDGETLVDGTTTNAELETLEDKNVRTGGG"
     gene            1175..2467
                     /locus_tag="VFO88_09700"
     CDS             1175..2467
                     /locus_tag="VFO88_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545841.1"
                     /translation="MRRFRIAGGLAAVAVLALGVTAPAAPAAAKKKVEVLAHVNPGVG
                     ANADVFAHRGHAYLGSWIGNGCLSSGVRIYDLKKPRSPRHVGTFADAAAEPDLAGTWT
                     EKVIVRRVNSRAFVGDLAVTSIQACSTSSSAFRGFGLYDVSDPSNPERLALVATEPQT
                     RGSHEIYMQRKGNRAYVYTAIISSEHLTSPDGVTPGNPDFRIYDVSNPRSPAQVGAWG
                     AWAELGVHPMDGPPGLNRNHVHSVIVDGNARRAYLSYWDLGTVILDITDPTDPEFLGR
                     TTFQPGEVGNAHSAWLAKGGKILVETHESFGAPGSPNVGVPTFWDISNPMSPIKLSTF
                     SRPDLIDLGETVHDPKVRGNTLFLSWYQEGVVVLDISRPSAPKLLTQFVPPASFVNPD
                     WSLCVGAPPCSSIWGVFLHRNYVLASDEAQGLWVLRFK"
     gene            complement(2704..2910)
                     /locus_tag="VFO88_09705"
     CDS             complement(2704..2910)
                     /locus_tag="VFO88_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545842.1"
                     /translation="MDDLLRFLRRLGHEADAVADGVVEVGPSGGTVDPGSVFPLAVSL
                     QLRVWNLVNETDARVVEVNEPLEP"
     gene            complement(3103..5031)
                     /locus_tag="VFO88_09710"
     CDS             complement(3103..5031)
                     /locus_tag="VFO88_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545843.1"
                     /translation="MIACLGTLILSAPALAQSSGQAQTIKQGALTAQGAEANAEANQN
                     AVNANVPVTIAGGDVKAGSSSATQNATNAANAGAGNTAATAQIVKAVQSGGSSSCYAG
                     CGGSGQYQAVGQAAGTKQNAEANAEANQNAVNANVPVTIAGGDVKAGSSSATQNAENE
                     ANAEAGNASLTAQAAGVKQSGGSSSCKKGCGGSGQYQAVGQLALTAQNADADADAKQN
                     AVNANTPVTIAGGDVETGSSSADQNAENEANAGAWNESATVQLVSAEQSGGGSSCYAG
                     CGGSGQYQAVGQIAATLQEAEADADANQNAVNGNAPVAIAGGDVKTGSSSATQNAENE
                     ANAGAGNASLTIQATEATQTGGPSWCHAGCGGSGQYQEILQAAATAQSADADADADQN
                     AVNGNAPVAIAGGDVYTGSSSADQNAENEANAGAWNESATVQLASAEQSGGGSWCSKG
                     CGGGGQYQAVGQFAATLQEAEADADAKQNAVNGNAPVAIAGDDVYTGSSSATQNAENE
                     ANAWAENASLTFQGAKVKQSGGSCKTRCGSPCEKGCSSPCKKRCFEYGDRCTWGCGGA
                     SQTQKVGQLAGTKQKADADADAKQNAVNGNAPVAIAGDDVYTGSSSADQNAENEANGW
                     AANKSLTIQAALAWQSLR"
     gene            complement(5131..6462)
                     /locus_tag="VFO88_09715"
     CDS             complement(5131..6462)
                     /locus_tag="VFO88_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545844.1"
                     /translation="MVFAPSVEQTAPRPRRGRARAKVAGALLAPLAALAGAGGGLAQP
                     PSLPAAPSVDGPGPAAALADAPAAAVAQASQTAQAAGAQATTTQQGPVNVAIQIIVNS
                     PGSSPVINQSNTAGSNAAAANGSSTTQGATGSGGAGGSTGQSAATGQNAAAGATTVQS
                     GATNVATTTVVESPSASPAITQANGAVSGSSATNSSSTTQAATGSGEATGAGAGAQPA
                     GDGTAPAPAQPSGTSGSQAPAVSVGGSGAGKLNDALWGWIWDAVEAALQPGGLGALVP
                     ALPPLPNLGFGGGVTLPDLPGLPGVWDPPAAAPKDARPHRAGEGRGERSAAAGGATAP
                     YAGTLPPAPSAYAIATGKPGPSLSSSFVQNGSDAGKTRRMELALPSVPSGPAPAGSAG
                     ALFSGAGLVLGALFLVALQLASAASALSRRFDLASIAWRRQAYLSPLERPG"
     gene            6813..8075
                     /locus_tag="VFO88_09720"
     CDS             6813..8075
                     /locus_tag="VFO88_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET9545845.1"
                     /translation="MLLHGAVTDRGDVAEGTIGSGGSAEEAEPLVAALKPAQRRVLAD
                     LAARGVDELTRAEYERIGLVSRSQAAYDLAELVQMGLLERIGSGRATQYRRAHPGPGR
                     KRRWTNDRIRAELEAFCAGRPDWPRAHDFRTAGRGDLYLAASRYGGIDHWAAELGYGT
                     ARGHAESRETRETRVVPSPPRRRSGRGIGRLAVLAVRPAVLAGAVLGLGLAGALAIAS
                     EYPAWNASRGTSVPVEPEVTLRPDPARNQAAPTASAVSSRAERDGAVLLLLHAARGSS
                     WVTVRRGSARGSIAFSGMLAQGATARVSGTRLWIRLDAAWNVDARVDGRTVDVRTARV
                     GIVTSRGVVIVARAHRPRPAAPPPPPPPAPVVVALAISSRSEATPPPPSPPPPPPAAS
                     QPVAATPQTPAPDPQPASGPGPDPEPRP"
     gene            8072..8701
                     /locus_tag="VFO88_09725"
     CDS             8072..8701
                     /locus_tag="VFO88_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9545846.1"
                     /translation="MTAAPSAASSLARAYRESGDAAARRRLIEQYMPLVVSLARRYTG
                     RGEQLEDLIQVGSEGLIKAVDRFEPDRGVQLGAFAIPTIVGEIKRHLRDRTWPVTVPR
                     HIRESHAISEYVVEPAAPERRPDEPPATPDPAWERSEQRALLASAWPALDERERRVLE
                     LRFFAELSQAEIARALGMTQIQVSRLVARSLRKMRAGIGERSDTNGGPT"
     gene            8698..9831
                     /locus_tag="VFO88_09730"
     CDS             8698..9831
                     /locus_tag="VFO88_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545847.1"
                     /translation="MSRFRRLRLLLPAAVAATIASLGLAGASAGSALDPTPPIITPVI
                     SGTLGDNGWHRSSVTVSWSVTDPESPIVEDDCRTRTQAQDTTGVPFSCWARNSAGLTG
                     AYTLVIKVDTTPPAASAALDRAADFDGWYTEPVSAVFSGTDQTSGIASCTAPVTYEGP
                     DTTGDEVPGTCRDKAGNQASAAVELRYDATAPVVSRIAAARRPDRYGWFNRPVRFVLN
                     GQDATSGIAGCESPSYAGPNDPRASVSGTCRDRAGNVSAPRAIRFRYARPLLDPVRGE
                     RVSAPVRLRWVDAKRASYYNLQLWRGSRKILSVWPRGTSYRVARSWAYHGERYALKPG
                     RRYRWYVWPKIGARYGKLLGSSNFQVRRRATAGVEPLRLGPVG"
     gene            complement(9852..10178)
                     /locus_tag="VFO88_09735"
     CDS             complement(9852..10178)
                     /locus_tag="VFO88_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545848.1"
                     /translation="MAVDHLLGDDPGLEDPWAFVLTAAIILAVATVLFATVVRPSLAP
                     RRAPLVLAILAVLSLAFIWIGLPFAAAPAAVALGLRVRAARNRGDRGRCRRPAARNRR
                     LRLPGG"
     gene            10340..10573
                     /locus_tag="VFO88_09740"
     CDS             10340..10573
                     /locus_tag="VFO88_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545849.1"
                     /translation="MRRLFVMSALALLAAALWVSPASAQVPPHEHILTTPSGDVDVGP
                     PRCGNEQLQGAFLNFHFNVHLGAPPTVIMPRFC"
     gene            complement(10738..10815)
                     /locus_tag="VFO88_09745"
     tRNA            complement(10738..10815)
                     /locus_tag="VFO88_09745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10778..10780),aa:Pro,seq:ggg)
     gene            complement(10871..11269)
                     /locus_tag="VFO88_09750"
     CDS             complement(10871..11269)
                     /locus_tag="VFO88_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545850.1"
                     /translation="MTREPVNGRLIVLCERLAESTRAGAAEWDDEGNDRFLWRHAEGS
                     VRVGARDGDGEPPYELTISNDREVRVEELESALLGDDVPAPWNEPLADLYRTARRSAL
                     KADELIDALIAALPGDAGGGRRSTASLTAD"
BASE COUNT         1470 a         4216 c         4044 g         1646 t
ORIGIN      
        1 tccggacgcg ctccccgcga acgtgacgat cgcgcacaac acgatcctga gcggggcgag
       61 gcgcggcttc gggcagtacc ccgacctcgc gaactcgctc gtcgtctcgc cgcgctacgg
      121 ctggaacccg gagtcgcagc gcccgctcgt cgtgaacaac gtcatcgcgc tcacgggaac
      181 gcccgacgag ctctgcggcc tcgcccggac ggcccggaac gtgacgatcc gcggggaggc
      241 ctgcgcggag gaggacgtcg tcggcgaccc gctcctgacc gacgccggcg tccccggccc
      301 ccactccacg ctcgtcgtcg accgcgggct accgggcttc tcggccgtcg acctcctcgg
      361 ccgcccgcgc gacgaggcgc cggacgtcgg cgcgtacgag gtcatgcgct gaaaccgggc
      421 gccgcggggt cgcgtattcc cgggcgacat ccgattcgag ggggtagccg catgcgaggg
      481 accggatcgt tccgtcgttc cgcgaccgtg atcgccgcgc tcgcgggagc gctcgtcctg
      541 ccggccgggg catcggcctc ggtcgagacg ttcgagcgct tcgaggccgt cgccaccgcg
      601 aacttcaccg tgacggagca gtgcgccgac ggctcgaccg tgcagacgct cgtcaccgtg
      661 ctcggaggcc acgaggtgga ggtggaatcg ggtgagacga cgctcgacaa cgacttcctc
      721 accgtcctcg ttcgggggtt cgactgctcc ggcgccttcg tgaacgaccg cggctcgggg
      781 ccggccacct tcacgttctc gccgtcgctc cagacggcct cggtcacggg gacgatcacg
      841 acgcgcgacg gccggacggt cagcgcggac gtcgcgtggg aagggacggg cgcgctcgag
      901 gcctcgtcga acacgacgac gttccccggc ttcacgggcc acttccgcag cacccgtcgc
      961 gacgcgatcg cgaccgggac ggtcgtcgtc gacggcgaga cgctcgtgga cgggacgacg
     1021 acgaacgccg agctcgagac gctcgaggac aagaacgtcc gcacgggcgg cggataggcc
     1081 gaggctgcat ggagcccgca tgcgaccgtt ggtacgatcc gcgccggtgt cgatcttgcg
     1141 gggcgcattc ggcgccctgc ggaggggaga tccgatgaga cggttccgca tcgccggggg
     1201 tctcgccgcc gtcgccgtgc tcgcgctcgg cgtcacggcc cccgctgcgc cggccgcggc
     1261 caagaagaag gtcgaggtcc tcgcgcacgt gaacccgggc gtcggcgcga acgccgacgt
     1321 cttcgcccac cgcggtcacg cctacctcgg gagctggatc gggaacggct gcctctcgag
     1381 cggcgtccgc atctacgacc tgaagaagcc tcgctcgccg cgccacgtcg gcacgttcgc
     1441 cgacgcggcc gccgagcccg acctcgccgg gacatggacg gagaaggtga tcgttcggcg
     1501 cgtcaactcg agggcgttcg tcggcgacct cgccgtgacc tcgatccagg cctgctcgac
     1561 ctcgtcgagc gccttccgcg ggttcgggct ctacgacgtg tccgatccgt cgaaccccga
     1621 gcgtctggcg ctcgtcgcga ccgagccgca gacgcgcggc tcgcacgaga tctacatgca
     1681 gcggaagggc aaccgcgcgt acgtctacac ggcgatcatc agctcggagc acctgacctc
     1741 gccggacggc gtcacgccgg ggaacccgga cttccggatc tacgacgtct cgaacccgcg
     1801 gagccccgcg caggtcggcg cctggggcgc ctgggccgag ctcggcgtgc atccgatgga
     1861 cgggccgccg gggctcaacc gcaaccatgt ccactccgtg atcgtcgacg ggaacgcccg
     1921 gcgcgcgtac ctctcgtact gggatctcgg gacggtcatc ctcgacatca ccgatccgac
     1981 cgaccccgag ttcctcggcc ggacgacctt ccagcccggc gaggtcggga atgctcactc
     2041 cgcgtggctc gcgaagggcg ggaagatcct cgtcgagacg cacgagtcgt tcggcgcacc
     2101 cgggagcccg aatgtcggcg tcccgacgtt ctgggacatc tcgaacccga tgagcccgat
     2161 caagctctcc acgttctcgc gacccgacct catcgacctc ggggagacgg tgcacgatcc
     2221 gaaggtgcgc gggaacacgc tgttcctctc ctggtaccag gagggcgtcg tggtgctcga
     2281 catctccagg ccgtcggcgc cgaagctcct gacgcagttc gtcccgccgg cctcgttcgt
     2341 gaaccccgac tggtcgctct gcgtcggcgc gccgccgtgc tcgtcgatct ggggcgtctt
     2401 cctgcaccgc aactacgtgc tcgcctcgga cgaggcacag ggcctctggg tcctccgctt
     2461 caagtagcgg cgagcgcatc ccggcatcac gcgagggccc ccgcgggagg gggccctcgc
     2521 acgttccggg tcgctctcgg aggggcgccg ggcggccccg gcgctgcggc tcgcccgggg
     2581 cacgccacga cgcccggtcc ccaccctagg ggcacggttc gaatcttgag gcgcaggaga
     2641 tagacgatcg gggtctcggg cgggtgcggc acgcccgcga ccccacttct tcgtgcccgg
     2701 ccgctacggc tcgagcggct cgttgacctc cacgacgcgc gcgtccgtct cgttcacgag
     2761 gttccacacg cgcagctgga gcgacacggc aagcgggaac acgcttccgg ggtcgaccgt
     2821 gcctccactc gggccgacct cgacgacgcc gtccgcgacc gcgtcggcct catggccgag
     2881 ccggcgcagg aagcgcagga ggtcgtccac ccgatgaggt gccgcagaga ctttcatgag
     2941 ccgcagagcg cgaccggaac gccattccgc cgcgctctcc cagtgttgcg ggcgccgccg
     3001 agacccgcaa tcccccaaac gtagtggacg cacagccgaa aacgccggga ggaggcgggg
     3061 cggtcgcgcc gtgccgaccg ccccgccccc ggctacggct cgctagcgga ggctctgcca
     3121 ggcgagcgcg gcctgaatcg tcaggctctt gttcgccgcc cagccgtttg cctcgttctc
     3181 ggcgttctga tccgccgagc tcgagccggt gtagacgtcg tcaccggcga tcgcgacggg
     3241 cgcgttgccg ttgacggcgt tctgcttcgc gtccgcgtcg gcgtccgcct tctgcttcgt
     3301 gccggcgagc tggccgacct tctgcgtctg gctcgcgccg ccgcagcccc acgtgcagcg
     3361 gtcgccgtac tcgaagcacc gcttcttgca cggggaggag cagcccttct cgcacgggct
     3421 gccgcaccgc gtcttgcagg aaccgccgga ctgcttcacc ttcgctccct ggaaggtgag
     3481 gcttgcgttc tcggcccatg cgttcgcctc gttctccgcg ttctgcgtcg cggagctcga
     3541 gccggtgtag acgtcgtcac cggcgatcgc gacgggcgcg ttgccgttga cggcgttctg
     3601 cttcgcgtcc gcgtccgcct cggcctcctg gagcgtcgcc gcgaactggc cgacggcctg
     3661 gtactggccg ccgccgccgc agcccttcga gcaccaggag ccgccgccgg actgctccgc
     3721 cgaggcgagc tggaccgtgg cgctctcgtt ccacgccccg gcgttcgcct cgttctcggc
     3781 gttctggtcg gccgagctcg agccggtgta cacgtcgccg ccggcgatcg cgacgggcgc
     3841 gttgccgttg acggcgttct gatcggcgtc ggcgtcggca tccgccgact gcgcggtggc
     3901 cgcggcctgg aggatctcct ggtactggcc gctgccgccg cagcccgcgt ggcaccacga
     3961 cgggccgccg gtctgcgtcg cctcggtcgc ctggatcgtg aggctggcat tcccggcacc
     4021 tgcatttgcc tcgttctccg cgttctgcgt cgcggagctc gagccggtct tcacgtcgcc
     4081 gccggcgatc gcgacgggcg cgttgccgtt gacggcgttc tggttcgcgt ccgcgtccgc
     4141 ctcggcctcc tgcagcgtcg ctgcgatctg gccgacggcc tggtactggc cgctgccgcc
     4201 gcagccggcg tagcaggacg acccgccgcc gctctgctcc gccgagacga gctggaccgt
     4261 ggcgctttcg ttccaggctc ctgcgttggc ctcgttctcg gcgttctggt cggccgagct
     4321 cgagccggtc tcgacgtcgc cgcccgcgat cgtcacgggc gtgttggcgt tgacggcgtt
     4381 ctgcttcgca tcggcgtccg cgtcggcgtt ctgcgcggtg agggcgagct ggccgacggc
     4441 ctggtactgg ccgctgccgc cgcagccctt cttgcacgac gacgagccgc cggactgctt
     4501 gacgccggcc gcctgggcgg tgaggctggc attgccggcc tcggcgttcg cctcgttctc
     4561 ggcgttctgc gtcgccgagc tcgagcccgc cttgacgtcg ccgccggcga tcgtgacggg
     4621 cacgttcgcg ttcacggcgt tctgattcgc ctccgcgttg gcttccgcgt tctgcttcgt
     4681 cccggccgcc tggccgacgg cctggtactg gccgctgccg ccgcaaccgg cgtagcagga
     4741 cgagctgccg ccgctctgga cggccttgac gatctgggcc gtcgcggccg tgttcccggc
     4801 gcccgcattc gccgcgttcg tggcgttctg cgtggccgag ctcgagcccg ccttcacgtc
     4861 gccgccggcg atcgtgacgg gcacgttggc gttcacggcg ttctggttcg cctcggcgtt
     4921 cgcctcggcc ccctgcgccg tgagtgctcc ctgcttgatc gtctgcgcct ggcccgagct
     4981 ctgggcgagg gcgggagcgc tcaggatgag cgtgcccaga caggcgatca agagtgcgat
     5041 cagtttgtgt ctcatcacca ctccttgttc gagttgtccc atcgcgccgg gggtcgactg
     5101 cacccggcgt cacggcaaca cgaacgggcg tcagccgggc cgctcgagtg gtgagaggta
     5161 ggcctggcgc ctccaggcga tggacgccag gtcgaagcgc cggctgagcg ccgaagcagc
     5221 cgacgcaagc tgaagggcga cgaggaagag cgcgccgagc acgaggccgg cgccgctgaa
     5281 gagcgcgccg gccgagccgg ccggtgcggg cccggacgga acgcttggaa gagcgagctc
     5341 catccggcgc gttttccctg catctgaacc gttctggacg aaactggacg aaaggctcgg
     5401 gcccggcttg cccgtcgcga tcgcatacgc ggagggtgcc ggaggaagcg tgccggcata
     5461 cggcgccgtc gccccgcccg cggccgcgct ccgctcgccg cgcccctcgc ccgcgcggtg
     5521 cgggcgggcg tccttcggcg cagcggctgg cggatcccag acgcccggca gcccgggcag
     5581 gtccggcagc gtcacgccgc cgccgaagcc gaggttcgga agcggcggga gggccggcac
     5641 gagcgcgccg aggccgcccg gctgcagagc cgcctcgacg gcgtcccaga tccagcccca
     5701 gagcgcgtcg ttcagcttcc cggcgcccga gcccccgacg gagacggcag gcgcctggga
     5761 gccggacgtc ccgctcggct gcgccggagc cggagccgtc ccgtccccgg ccggctgcgc
     5821 gcccgcgccg gccccagtcg cctcgccgga ccccgtcgcc gcctgggtcg tcgagctcga
     5881 gttcgtcgcg ctggagcccg agacggctcc gttcgcctgc gtgatcgccg ggctcgcgga
     5941 cgggctctca acgacggtgg tcgtcgcgac gttcgtcgca ccggactgca cggtcgtcgc
     6001 ccccgccgcg gcgttttgcc cggtcgcggc ggactgcccg gtcgacccgc ccgcaccgcc
     6061 cgaaccggtc gcgccctgcg tggtcgagct gccgttcgcg gccgcggcgt tcgacccggc
     6121 cgtgttcgac tggttgatca ccgggctgct ccccgggctg ttcacgatga tctggatcgc
     6181 cacgttcacg gggccctgct gcgtggtcgt cgcctgggcg cccgccgcct gggccgtctg
     6241 gctcgcctgt gcgaccgctg cggccggcgc atcggcgagc gcggcggccg gccccgggcc
     6301 gtccacggac ggcgcggcgg gcagcgacgg cggctgggcg agcccgccgc ctgcgcctgc
     6361 cagcgcggcg agcggagcga ggagggctcc ggcgaccttc gcgcgcgcgc gtccgcggcg
     6421 gggccgtggc gcggtctgct cgacgctcgg cgcgaaaacc atcctatgga acaggtaccc
     6481 acttgccgcg cggcgtttcg aatgccggcg agatttggac gagaaaagaa cgatggcgat
     6541 ttccgagcgc attcctcgtt cttctcggga attggacgaa ggcggttttc cgcgtcgtcc
     6601 ggtacctgcg ggcacagggc tccaggccgt ttcagtgcga ttggacgaga gggcggcggc
     6661 tcgggggaag agcgacgcgc gcggcctcgg cgccgtgccg tcggatcgag gccgcgcgtc
     6721 gtcgtaggcc gtgaatctgg tcttgacctg acttgtgtgt cgcggaacta tgtaggcgat
     6781 acgggacggc ggcatagggg agagcgcatg cggtgctcct ccacggcgcg gtgacggatc
     6841 ggggggacgt ggccgagggg acgatcggat ccgggggctc agccgaagag gccgagcctc
     6901 tggtcgcggc gctgaagccg gcgcagcgca gggttctcgc cgacctcgcc gcgcggggcg
     6961 tcgacgagct gacgcgggcc gagtacgagc gcatcgggct cgtcagccgc tcgcaggccg
     7021 cgtacgacct cgcggagctc gtccagatgg gcctcctcga gcgcatcggc tccggccgcg
     7081 cgacgcagta ccggcgcgcg catccgggcc ccggccgcaa acgccgctgg acgaacgacc
     7141 ggatccgcgc cgagctcgag gcgttctgcg ccgggcggcc ggactggcct cgcgcgcacg
     7201 actttcgcac ggccggccgc ggcgacctct acctcgcggc cagccgctac ggcgggatcg
     7261 accactgggc ggccgagctg ggctacggga cggcacgcgg acacgcggag agccgcgaga
     7321 cccgggagac ccgtgtcgtt ccgagcccgc cgcggcggcg ctccgggcgc gggatcggcc
     7381 ggctcgcggt tctcgcggtg cggccggcgg tgctggcggg cgccgtcctc gggctcgggc
     7441 tcgccggcgc gctggcgatc gcgtccgagt acccggcctg gaacgcgtcc cgcgggacgt
     7501 cggtgccggt cgagccggag gtcacgctcc gtcccgatcc cgcacgaaac caggccgctc
     7561 ctacggcctc cgcggtctcg agccgcgcgg agcgtgacgg cgccgtgctc ctcctgctcc
     7621 acgcggcgcg cggcagctcc tgggtgaccg tccggcgcgg ctcagcgcgg ggctcgatcg
     7681 ccttctccgg catgctcgcc cagggcgcga ccgcccgcgt gagcggcacg cggctctgga
     7741 tccgcctcga cgcggcctgg aacgtcgacg cacgcgtgga cggacggacg gtcgacgtgc
     7801 gcaccgcccg cgtcgggatc gtcacgagcc gcggcgtcgt gatcgtcgcc cgcgcgcacc
     7861 gcccgcggcc ggccgcgccg cctcccccgc ccccgcctgc tccggtcgtc gtcgcgctcg
     7921 cgatctcctc gcgctcggaa gcgacgccgc ctcctccgtc tcctccgccg cctccgccgg
     7981 ccgcgtctca gcccgtggcc gcaacgccgc agacgccggc tcccgacccg cagcccgcct
     8041 cggggccggg accggatcca gagccacgcc catgaccgcc gccccgtccg ccgcctcctc
     8101 gctcgccagg gcctaccgcg agtcgggcga cgcggccgcg cgccggcggc tcatcgagca
     8161 gtacatgccg ctcgtcgtga gcctcgcgcg ccgctacacg gggcggggcg agcagctcga
     8221 ggatctgatc caggtgggct ccgaggggct catcaaggcc gtcgaccgct tcgagccgga
     8281 ccgcggcgtc cagctcggcg cgtttgcgat cccgacgatc gtcggcgaga tcaagcgcca
     8341 cctgcgcgac cgcacctggc cggtgaccgt tccgcggcac atccgggagt cgcacgcgat
     8401 ctcggagtac gtcgtcgagc ccgcggcgcc cgagcggcga ccggacgagc cgccggccac
     8461 gccggacccg gcctgggagc gctcggagca gcgggccctg ctcgcctccg cgtggccggc
     8521 gctcgacgaa cgcgaacgcc gggtgctcga gcttcgcttc ttcgccgagc tgagccaggc
     8581 ggagatcgcc cgcgcgctcg gcatgaccca gatccaggta tcgaggctcg tcgcccgctc
     8641 gcttcgaaag atgcgggcgg ggatcggcga gcggtccgac acgaacggag gcccgacatg
     8701 agccggttcc gacgccttcg actgctcctc cccgccgccg tggcggcgac gatcgcgagc
     8761 ctcgggctcg ccggcgcgtc cgccgggagt gccctcgacc cgacgccgcc gatcatcacg
     8821 cccgtgatca gcgggacgct cggcgacaac ggctggcacc ggagcagcgt cacggtcagc
     8881 tggagcgtga ctgaccccga gtccccgatc gtcgaggacg attgccgcac ccgtacgcag
     8941 gcgcaggaca cgaccggtgt gccgttctcg tgctgggcga ggaactcggc cggcctgacc
     9001 ggcgcgtaca ccctcgtcat caaggtcgac acgacgccgc ccgccgcctc ggcagcgctc
     9061 gaccgcgcgg ccgacttcga cgggtggtac acggagccgg tctccgccgt cttcagcggc
     9121 accgaccaga cctccgggat cgcctcgtgc acggcgccgg tgacgtacga gggcccggac
     9181 acgaccggag acgaggttcc gggaacctgc cgcgacaagg cggggaacca ggcctcggcc
     9241 gccgtcgagc tgaggtacga cgcgacggcg cccgtcgtga gccggatcgc cgccgcgcgt
     9301 cgccccgacc ggtacgggtg gttcaaccgg cccgtccggt tcgtcctgaa cggacaggac
     9361 gcgacctccg ggatcgccgg ctgcgagtcg ccgagctacg ccgggccgaa cgacccgcgc
     9421 gcgtcggtct ccgggacgtg ccgcgaccgc gccgggaacg tgagcgcgcc gcgcgcaatc
     9481 aggttccgtt acgcgcggcc gctcctcgac cccgtgcgcg gggagcgggt gagcgcgccc
     9541 gtccgtctcc gctgggtcga cgccaagcgg gcgagctact acaacctcca gctctggcgc
     9601 gggtcgcgga agatcctgag cgtctggccg cgcgggacgt cctatcgggt cgcgcgcagc
     9661 tgggcctatc acggcgagcg ctacgccctg aagccgggcc gccgctaccg ctggtacgtc
     9721 tggccgaaga tcggcgcgag gtacggcaag ctgctcggct cgagcaattt ccaggtgcgc
     9781 cgccgggcga cggcaggcgt ggagccgctc cggctgggcc ccgtcggctg agacggcgtc
     9841 acgagagctt gtcaaccgcc tggaagacgt aggcgccggt tgcgagcagc aggacgacgg
     9901 cacctaccgc gatcgccgcg gttgcgagcg gcccgtacgc gcaacccgag cgcgacggcg
     9961 gccggggccg cggcgaacgg gaggccgatc cagatgaagg cgaggctgag aacggccagg
    10021 atcgcgagga cgagcggcgc gcggcgcgga gcgagcgagg gccgaaccac ggtcgcgaac
    10081 agcaccgtgg cgacggcgag gatgatcgcg gcggtcagaa cgaaggccca cgggtcctcg
    10141 agcccgggat cgtccccgag gagatggtcg accgccatcg ccgcgagtcc aagggctacg
    10201 ccccccgccg ccagtaccca gtcgatccgc cgcaccgcgc tggaagtcga ggccagccga
    10261 actgcccgaa agtgcggttg cgtacgaacg cgcggcgcga tacgacccag cctccaacca
    10321 tctggcgaaa ggtggagcca tgcggagatt gttcgtcatg tcggcgctcg cgctcctggc
    10381 tgcggccctc tgggtctcgc cggcgagcgc gcaggtgccg ccgcacgagc acatcctcac
    10441 gacgccgtcc ggcgatgtcg acgtcggccc gcccaggtgc ggcaatgagc agctgcaggg
    10501 cgcgttcctg aacttccact tcaacgttca tctcggggcg ccaccgaccg tgatcatgcc
    10561 gcgcttctgc tgacgctggg gtagcgcgcg gggagcgtgg ccggagctcg attcccggcc
    10621 cctccccgcg tctgcacggc aagagcagga gcccgatgat ccttccgatc gcagcgcatg
    10681 aagcttccga ggcctggctc cagcaaacga aagccccgcg tgtgcggggc tttccgatgg
    10741 tcggggcgcc gagattcgaa ctcgggacct ccagtccccc agactggcgc gctaaccagg
    10801 ctgcgccacg ccccgtcggc gctcagcgta gcgaatcggg ttcaacgagc ccaactcggc
    10861 gccgtgcccg ttaatccgca gtcagcgagg cagtcgatcg gcgcccgcca ccggcgtcgc
    10921 ctggaagcgc ggcgatcagc gcatcgatga gctcgtccgc cttgagcgcg ctgcgccgcg
    10981 ccgtccggta gagatcggca agcggctcgt tccacggcgc cggaacgtcg tcgccgagaa
    11041 gcgccgactc gagctcctct acccgaacct cgcggtcgtt cgagattgtc aactcgtacg
    11101 gcggctcgcc gtcaccgtcg cgggcgccga ctcggaccga cccctccgcg tgccgccaaa
    11161 gaaacctgtc gtttccttcg tcgtcccatt ccgcggctcc cgcgcgcgta ctctccgcga
    11221 ggcgctcgca gaggacgatc aggcggccat tgacgggttc acgggtcatg acatcgttcc
    11281 tttccggccg gcttccggcc cctcgaactg gcgtgtctgg acgggttcct caccgaacgt
    11341 cagctgcatg tacaacgaga accagcgcct gcccct
//
LOCUS       DASTFC010000331         4944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_817151,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000331 DASTFC010000000
VERSION     DASTFC010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817151"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..958
                     /gene="arsM"
                     /locus_tag="VFO88_09755"
     CDS             167..958
                     /gene="arsM"
                     /locus_tag="VFO88_09755"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET9545851.1"
                     /translation="MTDALREQVRKRYAEAATAPASGCGSGSCCGEPAAEDARFGAAL
                     YGGEDVRELPEAAALASLGCGNPTAVAELREGETVLDLGSGGGIDVILSARRVGPTGR
                     AYGLDMTDEMLALAQRNAAEAGIGNAIFLRGEIESVPLPAASVDVVISNCVVNLSTDK
                     PAVLAEIARVLKPGGRVGISDVVAEDRLSPEERAERGSFVGCIAGALSVGEYTAGLEA
                     AGLEDVSVDFTHEVADGLHGAIVRARKPAHAPVPIPAERSAAGCC"
     gene            complement(1026..1754)
                     /locus_tag="VFO88_09760"
     CDS             complement(1026..1754)
                     /locus_tag="VFO88_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9545852.1"
                     /translation="MPKVLIIEDDDVIADGMSRHLVSAGFDPIWVNKGESGLARLRYE
                     NPDVCVLDLMLPGLDGWRLIETARREGIGTPIVVVSARGTEHDRVHALEIGADDYLVK
                     PFSMKELVARVRAAARRGVRAQGEERGEPIEAEGLRVDPLNVQAYLDGRSIELTPTEF
                     RLLYALALERGRVVTRDELLQKIWGRRQTRRDRTVDVFVRKLRDKLEALEPSHTFIQT
                     RYGVGYKFEPTPKDAAERASQARS"
     gene            1873..2823
                     /locus_tag="VFO88_09765"
     CDS             1873..2823
                     /locus_tag="VFO88_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9545853.1"
                     /translation="MSPVADDTLRLDAERGRRLVEESREFVLVLDEDERVVAASRRAR
                     QALEGIVEGARVTEEMLVSGSDRVPFRVPYEVDGKRETLLYLGDPGELAAYEELRAGF
                     TAAVSHELRTPLARLLAHLENAQLPDANVPELLDSARGEVAEIGELIDDVLFLSELES
                     GRALVGLGSTVALPILREVVEGSTDKAARAGVRLAVAGEEDVELPLRPRMIRVVATNL
                     VDNAIRYAGAGATLTLSVAHEGGDAVLAGSDDGIGVAEADLARLFERFYRTDRARASR
                     GTGLGLAIVKHVVNAAGGRVEAEGGRGAGLTVRCVFPASP"
     gene            2931..3911
                     /locus_tag="VFO88_09770"
     CDS             2931..3911
                     /locus_tag="VFO88_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9545854.1"
                     /translation="MRVAPRTLLLALLAAFVLVAAGCGRESESSDGTANAANESGEEL
                     AGSVQADGSSTVGPLTSAAAELYREVQPGVNVTVGISGTGGGFERFCRGETDISNASR
                     PIDEEEVALCEEAGIEFTELQVAIDALTVVVNQENDWATCLTVDQLNAIWSPDSTISN
                     WSEVPDADFPDVPMPLAGPGTDSGTFDYFTDEVNGEEGASRTDYTPSEDDNVIVQAVS
                     GDPGGLGYFGFTYYEENEDTLKAVEIDGGDGCVAPSAEAARSGDYSPLARPLFIYVKN
                     ESLERPEVDDFVRFFLDRNDDIAAAALYIPPSEEQKAEALATFEAATGAR"
     gene            3912..4844
                     /gene="pstC"
                     /locus_tag="VFO88_09775"
     CDS             3912..4844
                     /gene="pstC"
                     /locus_tag="VFO88_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9545855.1"
                     /translation="MEARDAALGAPPLRLRAERRRYGEDVVKGVLALCALISVATTVG
                     IVVALLEPSIEFFSEVSPLDFFGGTEWAATFTPARFGVLPLVAGTLAVTAWACLVCMP
                     LGLGAAIFLSEYAGPRVRRILKPALETLAGIPTVVYGFFALTFFTPLLGDVGIQVGTF
                     NVLSAGLVMGVMLIPTVASISEDAMAAVPQDLRDGAFGLGASRLQVSTRIVVPAAISG
                     IVASYVLAISRAIGETMIVLIAAGGIAQITADPRDPMQTMTAFIAATGIGDVPTGSIA
                     YKTIFAVGLTLFVFTLAMNVVAIRLVRKYREIYE"
BASE COUNT          671 a         1811 c         1776 g          686 t
ORIGIN      
        1 tcgagccgct ctcgctctcc cagcccaccg tgagccacca cctgaagaag ctcacggagg
       61 ccggcctgct cgagcgcgag cagcgcggcc gctgggccta ctactccctc tccggcgagg
      121 cgcgcgagcg gctcgcttcg ctcctcgact tcacggaggt accggcatga cggacgccct
      181 gcgggaacag gttcgcaagc gctacgcgga ggcggcgacc gcgccggcct cgggctgcgg
      241 cagcgggagc tgctgcggcg agccggccgc ggaggacgcc cgcttcggcg cggcgctcta
      301 cggcggcgag gacgtgcgcg agctgccgga ggccgcggcg ctcgcgagcc tcggctgcgg
      361 gaacccgacc gcggtcgcgg agctccgcga gggcgagacc gtgctcgacc tcggctccgg
      421 cggcgggatc gacgtgatcc tctccgcgcg ccgggtgggc ccgaccgggc gcgcgtacgg
      481 gctggacatg acggacgaga tgcttgccct cgcacagcgg aacgcggccg aggcgggcat
      541 cgggaacgcg atcttcctgc gcggcgagat cgagagcgtg ccgctgcccg ccgcgtccgt
      601 cgacgtcgtc atctcgaact gcgtcgtgaa cctctcgacg gacaagccgg ccgtgctcgc
      661 ggagatcgcc cgcgtgctca agccgggcgg gcgcgtcggg atctccgacg tcgtcgccga
      721 ggaccgcctc tcccccgagg agcgtgcgga gcgcggctcg ttcgtcggct gcatcgcggg
      781 cgcgctctcc gtcggcgagt acaccgcagg gctcgaggcg gcggggctcg aggacgtctc
      841 ggtcgacttc acgcacgagg tcgccgacgg cctccacggc gcgatcgtcc gcgcccgcaa
      901 gccggcccac gccccggtcc cgatcccggc cgagcggtcg gcggcgggct gttgctgacg
      961 cgaaccgcga gaggaggtgg cggaatgacc ccggtctcgg agtgtgactg cggcggcgcc
     1021 tgctgctagg agcgcgcctg ggacgcccgc tcggcggcgt ccttcggggt cggctcgaac
     1081 ttgtagccga ccccgtagcg cgtctggatg aacgtgtgcg acggctcgag cgcctcgagc
     1141 ttgtcgcgga gcttccgcac gaagacgtcc acggtgcggt cgcggcgggt ctgacgccgg
     1201 ccccagatct tctggaggag ctcgtcccgc gtgaccaccc gcccgcgctc gagcgcgagc
     1261 gcgtagagga gccggaactc cgtcggcgtc agctcgatcg agcggccgtc gaggtacgcc
     1321 tggacgttca gcgggtcgac gcgcaggccc tccgcctcga tcggctcgcc gcgctcctcg
     1381 ccctgcgccc gcacgccgcg ccgcgcagcc gcccgcacgc gggcgacgag ctccttcatc
     1441 gagaacggct tcacgaggta gtcgtccgcg ccgatctcga gcgcgtgcac ccggtcgtgc
     1501 tcggtcccgc gggcgctgac gacgacgatc ggcgtcccga tcccctcgcg gcgcgcggtc
     1561 tcgatcagcc gccagccgtc cagcccgggc agcatgaggt cgagcacgca gacgtccggg
     1621 ttctcgtagc ggagccgcgc gagcccggac tcgcccttgt tcacccagat cgggtcgaac
     1681 cccgcggaga cgaggtgacg cgacatcccg tccgcgatca cgtcgtcgtc ctcgatgatg
     1741 agcaccttgg gcattgcccg atgcgaataa tggccgtgcg gggcacaccc gcgggtctcc
     1801 cggcggtgaa gaagcggtga acaggtggtg aagaggagct ggaacccgtt ccgccgggcg
     1861 gaagaacagc ccgtgagccc cgtcgcggac gacacgctgc gcctcgacgc cgagcgcggg
     1921 cgacggctcg tcgaggagag ccgcgagttc gtgctcgtgc tcgacgagga cgaacgcgtc
     1981 gtcgcggcca gccggcgcgc ccggcaggcg ctcgagggca tcgtcgaggg cgcccgcgtg
     2041 acggaggaga tgctcgtgag cggctccgac cgggtgccgt tccgcgtccc gtacgaggtc
     2101 gacggcaagc gcgagacgct cctctacctc ggcgacccgg gcgagctcgc cgcctacgag
     2161 gagctccgcg ccgggttcac cgcggcggtc tcacacgagc tccgcacgcc gctcgcgcgg
     2221 ctcctcgccc acctggagaa cgcacagctc cccgacgcga acgtgcccga gctcctcgac
     2281 tcggcgcgcg gcgaggtggc cgagatcggg gagctgatcg acgacgtact cttcctctcg
     2341 gagctcgagt cgggccgtgc cctggtcggg ctcgggtcga cggtcgcgct gccgatcctc
     2401 cgcgaggtcg tggagggctc gacggacaag gccgcgcggg ccggcgtccg gctcgcggtc
     2461 gcgggcgagg aggacgtcga gctcccgctc cggccgcgga tgatccgtgt ggtcgcgacg
     2521 aacctcgtcg acaacgcgat ccggtacgcc ggcgccgggg cgacgctcac gctctcggtc
     2581 gcccacgagg gcggcgatgc ggtgctcgcc ggctcggacg acgggatcgg cgtcgcggag
     2641 gccgacctcg cccgcctctt cgagcgcttc taccgcacgg accgcgcgcg ggcgtcgcgg
     2701 ggcaccggcc tcgggctcgc gatcgtgaag cacgtcgtga acgcggccgg cgggcgcgtc
     2761 gaggccgagg gcggccgcgg cgccgggctc accgtccgct gcgtgttccc cgcctcgccg
     2821 tagcggcgtt cacacgaccg taaccactcc ttcacgacct ttcagcccat gcggcgcgcg
     2881 ccgagggcga cactcgcggc gatgccaaga cgcgaaagga gaacgcccac gtgcgcgtag
     2941 cccccaggac gctactgctc gccctgctcg ccgccttcgt gctcgtcgcc gcaggctgcg
     3001 gccgcgagtc cgagagctcg gacgggaccg cgaacgccgc gaacgagtcc ggcgaggagc
     3061 tcgccgggag cgtgcaggcc gacggctcca gcacggtcgg cccgctgacg agcgccgccg
     3121 ccgagctcta ccgcgaggtg cagcccggcg tgaacgtcac cgtcgggatc tccggcaccg
     3181 gcggcggctt cgagcggttc tgccgcggcg agaccgacat ctcgaacgcc tcccgcccga
     3241 tcgacgagga ggaggtcgcc ctctgcgagg aggccgggat cgagttcacc gagctccagg
     3301 tcgcgatcga cgccctcacc gtcgtcgtga accaggagaa cgactgggcc acctgcctga
     3361 cggtcgacca gctaaacgcg atctggagcc ccgactccac gatctcgaac tggagcgagg
     3421 tgcccgacgc ggacttcccg gacgtcccga tgccgctcgc cggccccggc accgactcgg
     3481 ggacgttcga ctacttcacc gacgaggtga acggcgagga gggcgcgagc cgcaccgact
     3541 acacgccgag cgaggacgac aacgtgatcg tccaggccgt ctccggcgat cccggcgggc
     3601 tcggctactt cggcttcacg tactacgagg agaacgagga cacgctcaag gccgtcgaga
     3661 tcgacggcgg cgacggctgc gtcgccccga gcgccgaggc agcgcgctcc ggcgactaca
     3721 gcccgctcgc ccggccgctc ttcatctacg tgaagaacga gtcgctcgag cgaccggagg
     3781 tcgacgactt cgttcgcttc ttcctcgacc ggaacgacga catcgcggcg gccgcgctgt
     3841 acatcccgcc gtccgaggag cagaaggcgg aggcgctcgc gacgttcgag gcagcgacgg
     3901 gggcgcggta ggtggaggca cgcgacgcag cgctcggcgc gccgcccctc aggctgcgcg
     3961 ccgagcggcg gcgctacggc gaggacgtcg tcaagggcgt cctcgccctc tgcgcgctca
     4021 tctcggtcgc cacaaccgtg gggatcgtcg tcgcgctgct cgagccgtcg atcgagttct
     4081 tcagcgaggt ctcgccgctc gacttcttcg gcgggacgga gtgggcggcg acgttcacgc
     4141 cggcgcgctt cggcgtcctg ccgctcgtcg ccgggacgct cgccgtcacg gcctgggcct
     4201 gcctggtctg catgccgctc gggctcggcg cggcgatctt cctcagcgag tacgcgggcc
     4261 cgcgcgtccg gcggatcctg aagcccgcgc tcgagacgct cgccgggatc ccgaccgtcg
     4321 tctacggctt cttcgccctg acgttcttca ccccgctgct cggcgacgtc ggcatccagg
     4381 tcgggacgtt caacgtcctg agcgccgggc tcgtgatggg cgtcatgctc atccccaccg
     4441 tcgcctcgat ctccgaggac gcgatggccg cggtgccgca ggacctccgc gacggggcgt
     4501 tcgggctcgg cgcgagccgc ctccaggtgt cgacgcggat cgtcgtcccc gcggcgatct
     4561 ccgggatcgt cgcgagctac gtgctcgcga tctcgcgggc gatcggcgag acgatgatcg
     4621 tcctcatcgc cgccggcggg atcgcgcaga tcaccgccga cccgcgcgac ccgatgcaga
     4681 cgatgaccgc gttcatcgcg gcgaccggga tcggcgacgt cccgaccggc tcgatcgcgt
     4741 acaagacgat cttcgccgtc ggcctcacgc tcttcgtctt cacgctcgcg atgaacgtcg
     4801 tcgcgatcag gctcgtccgc aagtaccggg agatctacga atgagcgccc agccggtcga
     4861 cctgcgcggc ggcgacccgc gcgagcgcgc gaagggcgct gtcttctacg cccttctcgt
     4921 cttctcggtc gcggtcgggt tcgt
//
LOCUS       DASTFC010000332         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_240474,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000332 DASTFC010000000
VERSION     DASTFC010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240474"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VFO88_09780"
     CDS             complement(<1..1543)
                     /locus_tag="VFO88_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9545856.1"
                     /translation="MTEPFGERVVWQPAKPRFRPVRLVLAALISALAVLAAAALLPGV
                     RVEGLGGALLVAAVVSVLNALLPPLLAALRLPFTVVLGFLAVLLTNAAMLWAASSLLE
                     PRFAVDSFWWALVAALVVAAVSLVLDVIVGANDDDAYTLRVTQRIARRSGERVESDVP
                     GILFLEIDGLALPVLRRAMRDGSAPNMARWVAAGDYRLVEWETDLSSQTGASQAGILL
                     GSNEDIPAFRWVEKETGTLMTCSAPADCAEIERRRATGVGLLANGGASRGNLLSGEAD
                     QTILTVSRMEAEKKANPGYRAFFANGFNVTRTLVLFTWEVILELAAAARQRRRDVRPR
                     GHRGGIYPLMRGALCVVVRDLIVYGVLTDMMKGRPAVYATFSSYDEVAHHSGLERADT
                     LEALRKLDEQIGRIDRARRYAPRPYELVVLSDHGQTQGATFLQRNGYGLADLVDRSVG
                     RADVQGIAGGDEQQAIVGLAIDEASGASHAKKRAKNDVSGRRVVVLGSGNLGLVYFPE
                     DPRRLV"
     gene            complement(1540..2727)
                     /locus_tag="VFO88_09785"
     CDS             complement(1540..2727)
                     /locus_tag="VFO88_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HET9545857.1"
                     /translation="MNAPARGGLSRLVPFDERSRARRLAKVAAWLAGIAVVVALLGLL
                     GVDVVGWLESLWDTLTEISLAYLVAGLALQTVQTTLTALGWFAILRAGYPAAPLAYRH
                     VLAAYAAGIALNGFLPANIGTLVMLLMFVAIIPGASFPGVLGGMVVQKIFFTLAGTAV
                     YLYLFLSVPGSFELQLGGVHDHPALTLLTIVGGLALVALLARMFWRKVQGLWERAKEG
                     GAILATPRRYLVRVVAPSFVAWLAKLGVVGVFLAAYGIPVTFHTVMSVVGGNSLANTV
                     SATPGGIGVNQAVNAASLSDVTDTATATAYSVGQQLAVTAWNIVFALVLVVWAFGWTG
                     GRALVLDSYGDAKVKAAEQKAARAAKRAAKREDGGGRRLPGRRAASAPAADGDASEEE
                     GPS"
     gene            complement(2729..>3754)
                     /locus_tag="VFO88_09790"
     CDS             complement(2729..>3754)
                     /locus_tag="VFO88_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9545858.1"
                     /translation="AWATLVVVAVVLAIAAGLVGIWLVRETIVWLVAAGFLAFSIEPL
                     VRMSQRRGMGRGAATTVAFTLIALVVLVFALVVIPPVVDGARDLRDSIPAYVEQLQDT
                     GAADALNADGAIETAGDVAEGSATFFERSGSVLDAVGALVSGGFAIFMIFTFTLYFLV
                     YGRDIVGRIERRLGPHARAPFRRALRETYDMNQGYWYGKFLIGVIAGLTTFATMKLLG
                     LPYAAPLSIFVGITDLIPNIGATLGTIPVVLVGLLEDPWKGVVAGAVLILYQQVENNL
                     ITPKVFKRTVEIHPFVSVVTVIAFTALFGIVGALIAVPVTKAIEIAIDAVQESRAARA
                     AHPAGEG"
BASE COUNT          605 a         1336 c         1362 g          451 t
ORIGIN      
        1 gcacgagtcg ccgcggatcc tcggggaagt agacgagccc caggttgccc gagccgagca
       61 cgacgacgcg ccggccgctc acgtcgttct tcgcgcgctt cttcgcgtgc gacgcgccgg
      121 acgcctcgtc gatcgcgagg ccgacgatcg cctgctgctc gtccccgccg gcgatcccct
      181 ggacgtccgc ccgcccgacc gagcgatcga cgaggtccgc gagcccgtat ccgttgcgct
      241 ggaggaacgt cgcgccctgc gtctggccgt ggtcggagag gacgacgagc tcgtacggcc
      301 gcggcgcgta ccggcgcgcg cggtcgatcc gaccgatctg ctcgtcgagc ttgcgcagcg
      361 cctcgagcgt gtccgcccgc tcgaggcccg agtggtgtgc gacctcgtcg tagctcgaga
      421 acgtcgcgta gaccgcggga cggcccttca tcatgtccgt caggacgccg tagacgatca
      481 ggtcgcggac gacgacgcag agcgcgccgc gcatgagcgg gtagatcccg ccgcggtggc
      541 cgcgcgggcg cacgtcgcgc cggcgctggc gggcggccgc ggccagctcg aggatcacct
      601 cccacgtgaa gaggacgagc gtgcgcgtga cgttgaaccc gttggcgaag aacgcgcggt
      661 agccgggatt cgccttcttc tccgcctcca tgcggctgac cgtcaggatc gtctggtccg
      721 cctcgccgga gagcaggttc ccccggctgg cgccgccgtt cgcgagcagg ccgaccccgg
      781 tcgcgcgacg ccgctcgatc tcggcgcagt cggcgggcgc ggagcacgtc atgagcgtcc
      841 cggtctcctt ctcgacccag cggaaggcgg ggatgtcctc gttcgagccg agcaggatcc
      901 ccgcctggct cgccccggtc tgggaggaga gatcggtctc ccactcgacg agccggtagt
      961 ccccggcggc gacccagcgg gccatgttcg gcgcgctccc gtcgcgcatc gcgcgccgga
     1021 ggacgggcag cgcgagcccg tcgatctcga ggaagaggat ccccggcacg tccgactcga
     1081 cgcgctcacc cgagcgccgc gcgatccgct gcgtgacccg caacgtgtac gcgtcgtcgt
     1141 cgttcgcgcc cacgatcacg tcgagcacga ggctgaccgc agcgacgacg agcgcagcga
     1201 cgagggccca ccagaacgag tccaccgcga agcgcggctc gagcaggctc gaggccgccc
     1261 agagcatggc ggcgttcgtg aggagcaccg cgaggaagcc gaggacgacg gtgaacggca
     1321 ggcggagcgc cgcgagaagc ggcggcagga gcgcgttcag gacggacacg accgcggcga
     1381 cgagaagcgc gcctccgagc ccctcgacgc ggacgcccgg caggagtgcc gccgcggcga
     1441 gcaccgcgag ggccgagatg agcgcggcga ggacgagccg cacgggcctg aaacgcggct
     1501 tcgcgggctg ccagacgacg cgctcgccga acggctcggt cacgacgggc cttcctcctc
     1561 ggaggcgtcg ccgtcggccg ccggtgcgga cgcggcgcgc cggcccggca gccttcgtcc
     1621 tccgccgtcc tcgcgcttcg ctgcccgctt cgcggcccgc gcggccttct gctcggccgc
     1681 cttcaccttc gcatccccgt acgagtcgag cacgagcgcg cggccgcccg tccagccgaa
     1741 ggcccacacg acgagcacga gcgcgaagac gatgttccag gccgtgaccg cgagctgctg
     1801 gccgaccgag tacgcggtcg cggtcgcggt gtcagtcacg tcgctgaggg acgccgcgtt
     1861 cacggcctgg ttcacgccga tcccgcccgg cgtggcggag accgtgttcg cgagcgagtt
     1921 cccgcccacg accgacatga cggtgtggaa cgtgaccggg atcccgtacg ccgcgaggaa
     1981 gaccccgacg accccgagct tcgcgagcca ggcgacgaac gacggggcga cgacgcgcac
     2041 gagatagcgc cgcggcgtgg cgaggatcgc gccgccctcc ttcgcccgct cccagaggcc
     2101 ctggaccttc cgccagaaca tccgcgcgag gagcgcgacg agcgccagcc cgcccacaat
     2161 cgtcaagagg gtcagggcag gatggtcgtg caccccgccg agctgcagct cgaacgaccc
     2221 ggggacggag aggaagaggt agaggtagac ggccgtcccg gcgagggtga agaagatctt
     2281 ctggacgacc atccctccga gaacgccggg gaaggacgcc ccggggatga tcgcgacgaa
     2341 catgaggagc atgacgagcg tgccgatgtt cgcgggcagg aacccgttca gggcgatccc
     2401 ggccgcgtac gccgcgagga cgtgccggta cgcgagcggg gcggccggat aaccggcccg
     2461 gaggatcgcg aaccagccga gcgccgtgag ggtcgtctgg acggtctgga gggcgagccc
     2521 ggcgacgagg taggcgagcg agatctcggt gagcgtgtcc cagaggctct cgagccagcc
     2581 gaccacgtcc acgccgagca ggccgagcag cgcgacgacg accgcgatcc ccgccaacca
     2641 ggcggcgacc ttcgcgaggc gtcgcgcgcg cgagcgctcg tcgaacggca cgagccgcga
     2701 gagcccgccg cgggccggcg cgttcacgtc atccctcccc ggcggggtgg gccgcccgcg
     2761 cggcccggct ctcctgaacg gcgtcgatcg caatctcgat cgccttcgtc accggcacgg
     2821 cgatgagggc cccgacgatc ccgaagagcg ccgtgaacgc gatgacggtc acgacggaca
     2881 cgaacgggtg gatctcgacc gtgcgcttga agaccttggg cgtgatcagg ttgttctcga
     2941 cctgctggta caggatcagc acggccccgg cgacgacgcc cttccacgga tcctcgagca
     3001 gcccgacgag cacgaccggg atcgtcccga gcgtcgcgcc gatgttcggg atgaggtcgg
     3061 tgatgccgac gaagatcgag agcggagcgg cgtacgggag gccgagcagc ttcatcgtcg
     3121 cgaacgtcgt gaggccggcg atcacgccga tcaggaactt cccgtaccag tagccctggt
     3181 tcatgtcgta cgtctcgcgc agggcccggc ggaagggcgc ccgggcgtgc gggccgagac
     3241 gccgttcgat gcggccgacg atgtcccgcc cgtagacgag gaagtagagc gtgaaggtga
     3301 agatcatgaa gatcgcgaaa ccgccggaaa cgagcgctcc gaccgcgtcg agaaccgacc
     3361 cggagcgctc gaagaaggtc gcgctcccct cggcgacgtc cccggccgtc tcgatcgccc
     3421 cgtcggcgtt cagggcgtcc gcggcccccg tgtcctggag ctgctcgacg tacgcgggga
     3481 tgctgtcgcg gaggtcgcgc gccccgtcca cgacgggagg gatgacgacc agggcgaaga
     3541 cgagcacgac gagggcgatg agcgtgaacg cgaccgtcgt cgccgcgccg cgccccatgc
     3601 ctcgccgctg ggacattcgc acgagcggct cgatcgagaa ggcgaggaag ccggccgcga
     3661 cgagccagac gatcgtctcg cgcacgagcc agatgcccac gagcccggcc gcgatcgcga
     3721 gcacgacggc gacgacgacc agcgtcgccc acgc
//
LOCUS       DASTFC010000333         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1328345,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000333 DASTFC010000000
VERSION     DASTFC010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328345"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1282)
                     /gene="pepT"
                     /locus_tag="VFO88_09795"
     CDS             complement(2..1282)
                     /gene="pepT"
                     /locus_tag="VFO88_09795"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET9545859.1"
                     /translation="MREDGYPSDLASELAPDALERFLRYARIDTQADPESDTAPSTAK
                     QLDLSRVLVDELRALGLDDAELDDQGYVLATLPGTVERDVPAIALLAHVDVSPDAPAA
                     GIEPQVVRYEGGRLPLPGDPAVALDPDRSPELLRHVGHELVTSDGTTLLGADDKAGIA
                     AIMAGAGHLLRHPELERGPVRICFTRDEEVGRGTDGLDLERLGAVAAYTLDGSTAGEI
                     QDETFSALEATVRFRGVAAHPGSAKGQLVNALKVAADFVASLPRDRLSPETTEGRGGF
                     LHPRRVSGSEEECTVVVILRDFDGELLLGHEELLQRLAGEAVAAHPGSAVEVEVVEQY
                     RNMKEVLDEHPFVVEAAQEATRRAGLEPTLGAARGGTDGSRLSERGLPTPNVFTGGQD
                     YHSRHEWLCVADLGAAAATVVELVRVWAERQPPE"
     gene            1583..1894
                     /locus_tag="VFO88_09800"
     CDS             1583..1894
                     /locus_tag="VFO88_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545860.1"
                     /translation="MHATIRRYDGVDQNRIVELTDKVNETLVPKLSKMQGFKGYYLID
                     GGNGVFSSLGLFETSDQAEESTKLVSTWIRDERLESLLPNPPKITSGRVMAQKNGFVA
                     V"
     gene            1997..>2620
                     /locus_tag="VFO88_09805"
     CDS             1997..>2620
                     /locus_tag="VFO88_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545861.1"
                     /translation="MIRRALLAAAAAALTVAPAAAAQTADETALAERYAPVVRLVEQE
                     EECGPGEPYEPIDVDLLFDEPTVALRGPWNAFDLLEIGPAAEDLRGRYEHHLDFPGHA
                     LDPRCDYERWSRRLTEDGEPAVYANVAAEEGYPGRVALQYWLFYVFNDWNNLHEGDWE
                     MIQLVFEADDAAEALAEEPVLVGYSQHEGAESAEWGDEKLELVDGRPV"
BASE COUNT          376 a          920 c          961 g          363 t
ORIGIN      
        1 gctactcggg cggctgacgc tcggcccaga cccgtacgag ctcgacgacc gtcgcggcgg
       61 cggcgccgag gtcggcgacg cagagccact cgtggcggga gtggtagtcc tggccgccgg
      121 tgaagacgtt cggggtgggg agcccgcgct cggagaggcg tgagccgtcc gtcccgcccc
      181 gcgcggcgcc gagcgtcggc tcgagccccg cccggcgcgt cgcctcctgc gccgcctcga
      241 cgacgaacgg gtgctcgtcg agcacctcct tcatgttgcg gtactgctcg accacctcga
      301 cctcgaccgc ggagccggga tgcgcggcga ccgcctcgcc ggcgagccgc tggagcagct
      361 cctcgtgacc gagcaggagc tcgccgtcga agtcgcgcag gatcacgacg accgtgcact
      421 cctcctccga cccggacacg cggcgcgggt ggaggaagcc cccgcggccc tcggtcgtct
      481 ccggcgagag gcggtcgcgc gggaggctcg cgacgaagtc ggcggcgacc ttgagcgcgt
      541 tcacgagctg ccccttcgcg ctgccgggat gggcggccac accgcggaag cggacggtcg
      601 cctcgagcgc gctgaacgtc tcgtcctgga tctcccccgc ggtcgagccg tcgagcgtgt
      661 acgcggcgac cgcgccgagc cgctcgaggt cgagcccgtc cgtgccgcgc ccgacctcct
      721 cgtcgcgcgt gaagcagatg cgcacggggc cgcgctcgag ctcggggtgg cgcaggaggt
      781 ggccggcgcc cgccatgatc gcggcgatcc cagccttgtc gtccgccccg aggagcgtcg
      841 tcccgtcgct cgtcacgagc tcgtgcccga cgtggcggag gagctcgggg ctccggtccg
      901 ggtcgagcgc gacggcgggg tcgcccggaa gcggcaggcg gccgccctcg tagcggacga
      961 cctgcggctc gatcccggcg gccggcgcgt cgggcgagac gtccacgtgg gcgaggagcg
     1021 cgatcgccgg cacgtcgcgc tcgaccgtgc ccgggagcgt ggccagaacg tagccctggt
     1081 cgtcgagctc agcgtcgtcg agcccgagcg cgcggagctc atcgacgagc acccgcgaga
     1141 ggtcgagctg cttcgccgtg ctcggcgccg tgtcggactc cgggtcggcc tgcgtgtcga
     1201 tccgcgcgta gcgcaggaac cgctcgagcg cgtccggcgc cagctcgctc gcgaggtcgg
     1261 aggggtagcc gtcctcgcgc atggcagcac cctagcgagg cggcggctcc gagcaggcgg
     1321 gcggtgagcc gaccgtttcg cgcatacccc gaacgtaaca atggcgccga aacggcttga
     1381 acgggtcgct ccggggcgcc cagggggaaa cgcgctctcc ggcggtgcga gatcggtgct
     1441 aggttggaat tgcgcgtttg gctctctagc gttgaagccg ccgaggctct gcaccttcct
     1501 cttcgtctgt cacacgcggc ccgaccgcag caggagtccg tccggatttc ctgtcctgtg
     1561 agatgaaccg aaggagcatc acatgcatgc gaccattcgc cgctacgacg gtgtggacca
     1621 gaatcgcatc gtcgagctga ccgacaaggt caacgagacg ctcgtcccga agctcagcaa
     1681 gatgcagggc ttcaagggct actacctgat cgacggcggc aacggcgtct tcagctcgct
     1741 cgggctgttc gagacgtccg accaggccga ggagtcgacc aagctcgtct cgacgtggat
     1801 ccgcgacgag aggctcgagt ccctgcttcc gaacccgccg aagatcacga gcggcagggt
     1861 catggcgcag aagaacggct tcgtagccgt ctaatagcac cactgcgtca ccgatcaccg
     1921 gagagggccc cgtcggggcc ctctctgcgt gtccgaccgg gcccgggcgc ttggaagatc
     1981 gtgacagact ggcgccgtga ttcggagggc gcttctcgca gccgccgccg cggctctgac
     2041 cgttgcgccc gccgccgcgg cgcagacggc cgacgagacg gcgctcgcgg agcgctacgc
     2101 gccggtcgtc cggctcgtgg agcaggagga ggagtgcggt cccggcgagc cgtacgagcc
     2161 gatcgacgtc gacctgctct tcgacgagcc gaccgtcgcc ctgcgcggcc cctggaacgc
     2221 cttcgacctg ctcgagatcg gcccggccgc cgaggacctg cgcggccgct acgagcacca
     2281 cctcgacttc cccggccacg cgctcgaccc gcgctgcgac tacgagcggt ggtcgcggcg
     2341 gctgacggag gacggcgagc cggccgtcta cgcgaacgtg gcggccgagg aggggtaccc
     2401 gggccgggtt gccctccagt actggctgtt ctacgtcttc aacgactgga acaacctcca
     2461 cgagggcgac tgggagatga tccagctcgt cttcgaggcg gacgatgcgg cggaggcgct
     2521 cgccgaggag ccggtgctcg tcgggtacag ccagcacgag ggcgccgagt cggccgagtg
     2581 gggagacgag aagctcgagc tcgtggacgg ccggccggtc
//
LOCUS       DASTFC010000334         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1624869,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000334 DASTFC010000000
VERSION     DASTFC010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624869"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(496..1341)
                     /locus_tag="VFO88_09810"
     CDS             complement(496..1341)
                     /locus_tag="VFO88_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9545862.1"
                     /translation="MELRRATPADFEAELRVLDAAEGGLRQRHGFDWRPPPLDAFRSA
                     HEYLLETDPDRSWVAVEDGEVVAYTAAWERDGHWFLSDLFVHPSVQAGGVGSALLERA
                     WGDGPERRLTLADAIQPVSNAMYARRGLVPATPMLELGGRPRAEPGSLEPATTEPEAL
                     AAVDAAAYGFDRARDHAHWSEVQPATVWLRGGEPAAYSYRTQSGRLGPIAGVDPAAAV
                     DALRSELSRAEGAVSLDVPGSARELVEAALAAGLRITGPPGLLLLSPGLAPPRALAIS
                     GYWLF"
     gene            complement(1341..2669)
                     /locus_tag="VFO88_09815"
     CDS             complement(1341..2669)
                     /locus_tag="VFO88_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9545863.1"
                     /translation="MPEPVIPEQWREELFELLRIPSVSADPAHAADVRRAAEWVCDFV
                     RAAGGECELVETDAQPLAVGEIRASSGAQDAPTVLVYGHVDVQPPAPLGAWESPPFEP
                     EIRGEWLYGRGVADDKGNLYLLLKAAALLAGEGALPVNVRVACDAEEEIGGHSIVDFL
                     ARDDRGADACLIFDGGMAKRDVPLFMVGTRGLAYFHVRLRTGERDLHSGIFGGAALNA
                     VHALADTLRAVTEVPQELRRGIVPPTPEEVAAWSELDPGAAVLAEQGARPKDERAAEE
                     FYARTFGDAAVDVNGIAGGEPLLQKTVLPVEAEANVSIRLAPGQDAEEIALAFERILR
                     EAAPPGAELDVERWSTAPAGLVDPEAPAVRLAQDAFERALGVRPLLVRSGGTLPIVPA
                     LADRGIPTVLSGFDLPEGNVHSPNERLLVRYVPLGVATARELFTGLAALR"
     gene            2722..3381
                     /locus_tag="VFO88_09820"
     CDS             2722..3381
                     /locus_tag="VFO88_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545864.1"
                     /translation="MRPLAAVALAVLVLAGCGGDPNAPSRNLREDDQRWVEGALPRAE
                     ILPEGWTGTADDRGAALDDCEALDRSDLELTGEAVASVELGESLAAIAGTQLFAGEED
                     ARAFVAEPSDEELTACLEEELAETFGDEERGAAVDGLAFVPSAPPDAGESARAATGEG
                     TYTVGETRSAIALEAMYVQRDRAVLTLVTAALDDEFPEDVRATLLADFEERTQEKPPP
                     P"
     gene            complement(3405..3584)
                     /locus_tag="VFO88_09825"
     CDS             complement(3405..3584)
                     /locus_tag="VFO88_09825"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HET9545865.1"
                     /translation="MALELRASCEQCGTALAAEGEAYICVHECTYCPSCTTALGAVCR
                     NCGGELVRRPRPPKP"
BASE COUNT          512 a         1413 c         1382 g          446 t
ORIGIN      
        1 gccgggagag tcgacggtct tcccctcgcc gagagcaacg accgggacga gcggcgagat
       61 gtcgccgccg tttccgtaga tcgcggcgag ctcgcggttc gtctcgtacc cctcctcgcc
      121 cggcacgggg aactcctgag aaagggcgct tcctgcgggt ccgataccgg cgaaggcggc
      181 gatcgtcacg acgatccaga gacccacgac cacgcgcttg tgggcgagaa cccagtcggt
      241 gaccctgtcg atgccaaccg aacgcgaagc ggtcgagctg ctgcgagctt ccatcgtgac
      301 cccctagaac ggcttcctgg gcaccggccg gtaccccagt gcgcgtttct cgaaggtcgc
      361 tctcgcccga agcgacgtaa gcggcggtcg agccggcgca ggcacgcaaa tgccgcgcgc
      421 gggtcagcgt agcgtgagcg tagcacccgc ttcagcgtcg tcgtggatga gcacgagcag
      481 ctcgcgaaca gcgcttcaga agagccagta gccggagatc gcgagcgcgc gcggcggcgc
      541 gaggccgggc gagagcagca ggagcccggg cgggccggtg atgcgcaggc cggcggcgag
      601 tgcggcctcg acgagctccc tcgcgctacc cggcacgtcg agcgacaccg ctccctccgc
      661 ccgcgacagc tccgagcgga gcgcgtcgac cgccgcggca gggtcgacgc cggcgatcgg
      721 cccgaggcgg ccggactgcg tccggtagga gtaggcggcc ggctcgccgc cgcggagcca
      781 gaccgtcgcc ggctgcacct cgctccagtg cgcgtggtcg cgcgcccggt cgaacccgta
      841 cgcggccgcg tcgaccgccg cgagcgcttc cggctcggtc gtagccggct cgagcgaccc
      901 gggctcggcg cgcggcctcc cgccgagctc gagcatcggc gtggccggga cgaggccgcg
      961 gcgcgcgtac atcgcgttcg agaccggctg gatcgcgtcg gcgagcgtca gccgccgctc
     1021 gggcccgtcg ccccacgcgc gctcgaggag cgccgacccc acgccgcccg cctgcacgga
     1081 cggatggacg aagaggtcgg agaggaacca gtgcccgtcc cgctcccacg ccgccgtgta
     1141 cgcgacgacc tcgccgtcct cgacggcgac ccacgagcgg tcgggatccg tttcgagcag
     1201 atactcgtgt gcggaccgga aggcgtcgag cggcggcgga cgccagtcga acccgtgccg
     1261 ctgccggaga ccgccctcgg ccgcgtcgag cacccggagc tccgcctcga agtccgccgg
     1321 ggtcgcccga cggagctcca tcagcgaagc gccgcgagcc cggtgaagag ctcgcgcgcc
     1381 gtcgcgacgc cgagcgggac gtagcgcacg agcagccgct cgttcggcga gtggacgttc
     1441 ccctcgggga ggtcgaagcc ggagagcacg gtcggaatgc cgcggtcggc gagcgccggg
     1501 acgatcggca gcgtcccgcc cgaccggacg agcagcgggc gcacgcccag cgcgcgctcg
     1561 aacgcgtcct gcgcgagccg caccgcgggc gcctcgggat cgacgagccc ggccggcgcc
     1621 gtcgaccagc gctcgacgtc gagctccgca ccgggcggcg cggcctcccg caggatccgc
     1681 tcgaacgcga gggcgatctc ctccgcgtcc tgccccggcg cgagccggat cgagacgttc
     1741 gcctccgcct cgacgggcag cacggtcttc tggagcagcg gctcgccccc ggcgatcccg
     1801 ttcacgtcga ccgccgcgtc tccgaacgtg cgcgcgtaga actcctcggc cgcgcgctcg
     1861 tccttcggcc gcgcgccctg ctcagcgagg acggccgccc ccgggtcgag ctccgaccac
     1921 gcggccacct cctccggcgt cggcggcacg atcccgcggc gcagctcctg cggcacctcc
     1981 gtgaccgcgc ggagcgtgtc ggcgagcgcg tgaacggcgt tcagcgcggc gccgccgaag
     2041 atcccggagt ggaggtcgcg ctcgcccgtc cgcagacgca cgtggaagta cgcgaggccc
     2101 cgcgtcccga ccatgaagag cggcacgtcg cgcttcgcca tcccgccgtc gaagatgagg
     2161 cacgcgtcgg cgccgcggtc gtcccgggcg aggaagtcga cgatcgagtg cccgccgatc
     2221 tcctcctcgg cgtcgcaggc gacgcgcacg ttcacgggaa gcgcgccctc cccggcgagg
     2281 agcgccgccg ccttgagcag gaggtagagg ttccccttgt cgtccgcgac gccgcgcccg
     2341 tagagccact cgccgcggat ctcgggctcg aacggcggcg actcccaggc gccgagcggc
     2401 gcgggcggct ggacgtcgac gtggccgtag acgagcacgg tcggggcgtc ctgcgccccg
     2461 ctcgaggcgc ggatctcacc gaccgcgagc ggctgcgcgt cggtctcgac gagctcgcac
     2521 tcgcccccgg cggcgcgcac gaagtcgcac acccactcgg ccgcgcgccg cacgtccgcg
     2581 gcgtgggccg ggtcggcgct gacgctcggg atgcgaagga gctcgaagag ctcctcgcgc
     2641 cactgctccg ggatgacggg ctcgggcacg cgccggagtc tatgcaggcg gccgcgcgcc
     2701 tccggggagt acgtttgggc cgtgcgcccc cttgcggccg tcgcgctcgc cgtcctcgtc
     2761 ctcgccggct gcggcggcga cccgaacgcg ccctcgcgca acctccgcga ggacgaccag
     2821 cgctgggtcg agggcgcgct cccgcgcgcg gagatcctcc cggagggctg gaccggcacg
     2881 gccgacgacc gcggcgccgc cctcgacgac tgcgaggcgc tcgaccgctc cgatctcgag
     2941 ctgaccgggg aggcggtcgc gtcggtcgag ctcggcgaga gtcttgccgc gatcgcgggc
     3001 acgcagctct tcgccggcga ggaggacgcg cgcgcgttcg tcgccgagcc ctccgacgag
     3061 gagctcacgg cctgcctgga ggaggagctc gccgagacgt tcggcgacga ggagcgcggc
     3121 gcggcggtcg acgggctcgc cttcgtcccg tcggcgccgc ccgacgcggg cgagagcgcg
     3181 cgggcggcga ccggcgaggg cacctacacg gtcggcgaga cccgctcggc gatcgcgctc
     3241 gaggcgatgt acgtccagcg cgaccgagcc gtcctcacgc tcgtcacggc agcgctcgac
     3301 gacgagttcc cggaggacgt gcgcgccacg ctcctcgcgg acttcgagga gcggacgcag
     3361 gagaagccgc cgcccccgta ggcaagtaac actgtgttac gaagtcaggg cttcggcggg
     3421 cgcgggcgcc ggacgagctc gccgccgcag ttccggcaga cggccccgag cgcggtcgtg
     3481 cacgacgggc agtacgtgca ctcgtggacg cagatgtagg cctcgccctc ggccgcgagc
     3541 gccgtgccgc actgctcgca cgacgctcgc agctcgagag ccaccagccg ctaggggacg
     3601 gggtgcgaaa ccaggtgacg gggctcgagg ccgcgccgcg ccatcaggcg gcgacctccc
     3661 gcagcttctg cgactcggcc atcgcctgga gcttcttcag gctctggttc tcgatctggc
     3721 ggacgcgctc gcgcgtcacg ttgaacgtct tgc
//
LOCUS       DASTFC010000335         7407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_916769,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000335 DASTFC010000000
VERSION     DASTFC010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916769"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFO88_09830"
     CDS             complement(<1..651)
                     /locus_tag="VFO88_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545866.1"
                     /translation="MEWDEHAARGRARYLDGEARLPDDPDARQRQLVRMAMAAGAVGL
                     ARLMQRRDDDAREWLVRSAERYRESWDTAPPGSWGRPIGALKARVLARDAAGAGHDAA
                     WTLAAVPADEASPIGVYAAVLALLVLGRDPEAAERAERLRGAEGFPADVGEALHALAL
                     GDAAAYAEAAASVLRSFEERDAYLEDIPVADTVLVLELLAAERGLAAGLRSPLLPRE"
     gene            645..1160
                     /locus_tag="VFO88_09835"
     CDS             645..1160
                     /locus_tag="VFO88_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HET9545867.1"
                     /translation="MPRRDPSAARYGLAVRETFASQAPWFVAGPLLGLCVVAVLALVN
                     GRLGVVGGFSDVVDRVSERSLRLGWKGTFLVGLVGGALLWALVSGGRAEEGYGWLTRT
                     FSDPIVALALLVAGICIGLGAKTAGGCTAGNGLSGNAFGSRAALVATMTFMAVAVAVS
                     FVTKWIWGAAL"
     gene            1157..1612
                     /locus_tag="VFO88_09840"
     CDS             1157..1612
                     /locus_tag="VFO88_09840"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HET9545868.1"
                     /translation="MTRLAAALVGVVFGFTLAWSGMSDPDVIRAGLLFEDLYLFSFFF
                     AAMATAFVGVQVLRRTNARALLTRDRITPETLRPEKRHVGGSILFGLGWAISAACPGP
                     VAAQLGQGILWSVATIAGIVIGLKIHAARGRARETTEAGARPAPVVGIE"
     gene            complement(1620..2069)
                     /locus_tag="VFO88_09845"
     CDS             complement(1620..2069)
                     /locus_tag="VFO88_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545869.1"
                     /translation="MGIGKLAVLMVAGAFAFGGIGAALADWARSDSAEAITLDDGRKD
                     DAGVELAAADDDDQKKKNKKKGGKGGDGDNTRGNDGTSGGNNGGDNTLSFSRDNNSRS
                     RAGANVAPAPAPVQASPPPAPAPQPAPAPADSYSRDSWSAPSGGGDT"
     gene            complement(2158..3069)
                     /locus_tag="VFO88_09850"
     CDS             complement(2158..3069)
                     /locus_tag="VFO88_09850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9545870.1"
                     /translation="MEPSYESWELAEGDEIVPGRYAVDLLGGGRRYEAYLTWDDELHA
                     LVVAKLLRPDLVEDGRALHGLAREAAALDALAHPSLVRGFGAVLGGDRPHVLLEYLDG
                     PRLSTLIRRYGVILEQLLPLALELTSALHYMAARGFVHLDVKPKNVVMSAPPRLIDLS
                     VAQRIEDIATLDSPIGTDPYMAPEQADPARFGELGPPADVWGLGVTLFEAITGELPFE
                     RPGDERLAPAERFPQISAAPAPLPRDLPPAVAELVLATLEKSPANRPVAGEVAAALEP
                     LVAALPAPRLGLFRPGGRTRQTEFLAG"
     gene            3176..4669
                     /locus_tag="VFO88_09855"
     CDS             3176..4669
                     /locus_tag="VFO88_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9545871.1"
                     /translation="MTSTAETDTGGKPGLRRRIRERIAGPRARLLRMPSSIRTRTIVW
                     FVGVLAVATLSSVLVTRGVLLIRLDQRVDSELTQEAAELRRLAGGNDPATGDPFRGDV
                     NRVFRVFLQRNIPAPYEAIVTFVDGEPFLRSRPVTAYRLDEDPEIADRWARLEEAERD
                     ALDTPEGRVEYLAVPLEFGGETRGVFVSAIFRDRARTEVDEATRAAAAVGLAVLLLGS
                     LLAWRLADRIVRPVTELTRTSRAITETEFSRRIPVEGQDEVAQLATTYNEMLDRLESA
                     FASQREFLDDVGHELKTPLTIVRGHLELLEDDPEERRETLALVLDELDRMARIVEDLL
                     VLAKHERPGFLRLETVDVGELTDAIYAKVAPLESAEWILENRGRGVIVADGQRLTQAV
                     LQLAQNAVRHAGGTIALGSQVENGEARFWVRDQGPGIDPEEQKTIFSRFRRGESAGRE
                     GSGLGLTIVRAIAERHHGRVEVASIPGIGSTFAVVVPVDQPHEEEIA"
     gene            4666..5328
                     /locus_tag="VFO88_09860"
     CDS             4666..5328
                     /locus_tag="VFO88_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9545872.1"
                     /translation="MSRILIVEDEARIASFVEKGLKANGFATRVAGDGESALGLVRAG
                     QYDLVILDIGLPGIDGFQVLEELREKRPALPVVILTARDSVGDTVAGLEGGADDYITK
                     PFRFEELLARVRLRLRGSRPAEPTVLEAGDVALDLRTRQAIVDGATYDLSAREFALAE
                     VFFRHPGQVLSREQLLSQVWGYDFDPGSNVVDVYVGYLRKKLGKRRIVSVRGMGYRFE
                     RE"
     gene            5464..6027
                     /locus_tag="VFO88_09865"
     CDS             5464..6027
                     /locus_tag="VFO88_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545873.1"
                     /translation="MTSKRKILEIGGVAAGVILIAFGIAAIVMGYNGRTEVRDSLARE
                     NIVGTPDSTIPGQKVDTGSEAKAFADVMRAHTLEITGGQTYAEMGRFLDEDGNPTNDE
                     AAAAVGDNGQPVENGLRQVWVTETALSTALNMSYMAEQLALFGIVVGVALLLSGIGFL
                     VLALGGALRHRETAAAASGTAARPLTT"
     gene            complement(6081..6410)
                     /locus_tag="VFO88_09870"
     CDS             complement(6081..6410)
                     /locus_tag="VFO88_09870"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET9545874.1"
                     /translation="MPYDEGLAERVRELLEERPDLTERKMFGGIGFMLGGNMCCGVLG
                     DELVVRLAAEDGEAALAEPHVRPFDFTGRPMKGFVLVGPAATETDEGLDAWVARAESF
                     ASSLPPK"
     gene            6440..7189
                     /locus_tag="VFO88_09875"
     CDS             6440..7189
                     /locus_tag="VFO88_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545875.1"
                     /translation="MRALLLPGIVTPAAVAYERLVPALGPEVDARPKELEVYAGPEPP
                     PGYSLEVEMDGVLRAADDAGWERFHLVGYSGGGAVAASLAASRPERLLSLGLLEPAWT
                     GNGGLSETEAKARAEFDALDGLEPREFLATFARLQLRPGVEPPPPPDPPPSWMATRPA
                     AIAALTLAFRTDELSAESLRRFDRPVYHALGGRSRPELYGEQSARLARLFPDFTEEVF
                     EERHHFDPPHRAEPERLAASLLAHWRRAEAG"
     gene            complement(7190..>7407)
                     /locus_tag="VFO88_09880"
     CDS             complement(7190..>7407)
                     /locus_tag="VFO88_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545876.1"
                     /translation="MTPEALFEGRKLLTLARIDYIVLLLVVLDMVVKPTGDDVALLVV
                     MAAILVAGVAYVLWRARSLTPPVAATT"
BASE COUNT          959 a         2711 c         2734 g         1003 t
ORIGIN      
        1 ctcgcgcggg aggagcggcg agcggaggcc ggcggcgagg ccgcgctcgg cggcgaggag
       61 ctccagcacg agcacggtgt ccgcgaccgg gatgtcctcg aggtacgcgt cccgctcctc
      121 gaaggagcgg agcacggacg ccgcggcctc ggcgtacgcg gccgcgtcac cgagcgccag
      181 cgcgtggagc gcctccccga cgtccgcggg aaacccctcc gccccgcgca gccgctccgc
      241 ccgctcggcc gcctcggggt cgcggccgag cacgaggagc gcgagcacgg ccgcgtagac
      301 gccgatcggc gacgcctcgt cggccggaac cgcggcgagc gtccacgcgg cgtcgtggcc
      361 cgccccggcc gcatcacgcg cgagcacgcg ggccttgagc gcgccgatcg gccggcccca
      421 gctccccggc ggcgccgtgt cccagctctc gcggtaccgc tcggccgagc gcacgagcca
      481 ttcccgcgcg tcgtcgtccc ggcgctgcat gagccgcgcg agccccacgg cgcccgcggc
      541 catcgccatc cggacgagct ggcgctgacg ggcgtccggg tcgtccggca ggcgcgcctc
      601 gccgtcgagg tagcgcgccc gcccgcgcgc cgcgtgctcg tcccattcca cgccgcgacc
      661 ctagcgccgc acggtacggt ctcgccgtgc gggagacgtt cgcgagccag gccccgtggt
      721 tcgtcgccgg gcccctgctc ggcctctgcg tcgtcgccgt gctcgcgctc gtgaacggga
      781 ggctcggggt cgtcggcggg ttctcggacg tcgtcgaccg cgtgtccgag cggagcctcc
      841 gcctcggctg gaaggggacg ttcctcgtcg ggctcgtcgg cggcgcgctc ctctgggcgc
      901 tcgtctcggg cggacgggcc gaggaaggct acggctggct cacgcggacg ttctccgacc
      961 cgatcgtcgc gctcgcgctc ctcgtcgcgg gcatctgcat cggcctcggc gcgaagacgg
     1021 ccggcggctg cacggccggg aacgggctct cggggaacgc cttcggctcg cgcgcggccc
     1081 tcgtcgccac catgaccttc atggccgtgg ccgtcgcggt cagcttcgtg acgaagtgga
     1141 tctggggagc ggccctgtga cccgcctcgc ggccgcgctc gtcggcgtcg tcttcggctt
     1201 cacgctcgcg tggtcgggga tgagcgaccc cgacgtcatc cgggccgggc ttctcttcga
     1261 ggacctctat ctcttctcgt tcttcttcgc cgcgatggcg accgcgttcg tcggcgtgca
     1321 ggtcctgcgc cgcacgaacg cccgggcgct cctcacgcgg gaccggatca cgcccgagac
     1381 gctccggccg gagaagcgcc acgtcggcgg gagcatcctc ttcggcctcg gctgggcgat
     1441 ctcggccgcc tgccccgggc cggtcgcggc ccagctcggg caggggatcc tctggagcgt
     1501 cgccacgatc gccgggatcg tgatcggcct caagatccac gccgcccgcg gacgggcccg
     1561 cgaaacgacg gaggccggcg cgcggccggc ccccgtcgtc gggatcgagt agcggtcgtc
     1621 tacgtgtcgc cgccgcccga gggcgccgac cagctgtccc gcgagtagct gtccgccgga
     1681 gcgggagcgg gctgcggggc cggagccgga ggcgggctcg cctggaccgg agccggggcc
     1741 ggagcgacgt tcgcgccggc gcgcgagcgg ctgttgttgt cgcggctgaa cgagagggtg
     1801 ttgtccccac cgttgttccc acccgacgtg ccgtcgttgc cgcgggtgtt gtcgccgtcg
     1861 ccgcccttgc cgcccttctt cttgttcttc ttcttctggt cgtcgtcgtc ggcggcggcg
     1921 agctccacgc cggcgtcgtc cttgcgaccg tcgtcgagcg tgatcgcctc ggccgagtcc
     1981 gacctcgccc aatcggcgag ggcggcgccg atgccgccga atgcgaatgc gccggcgacc
     2041 atgagaactg cgagctttcc gattcccatc atgacctcct tggcgtctgg attccgttga
     2101 cgaggtcagc atcgccgcgt gcaatgagac tcccgtgaga gcccggtgag agtcgtctca
     2161 tccggccagg aactccgtct ggcgcgtccg cccgcccggc cggaagaggc cgagccgggg
     2221 cgccgggagc gctgcgacga gcggctcgag cgccgcagcc acctcccccg cgaccgggcg
     2281 gttcgccggg ctcttctcga gcgtggcgag cacgagctcg gccaccgccg gcggcaggtc
     2341 gcgggggagg ggggcagggg ccgcggagat ctgggggaag cgctctgcgg gcgccaggcg
     2401 ttcgtcgccc ggccgctcga acgggagctc gcccgtgatc gcctcgaaga gcgtcacgcc
     2461 gagcccccac acgtcggcgg gcggcccgag ctcgccgaac cgcgccggat cggcctgctc
     2521 cggcgccatg tacgggtcgg tgccgatcgg gctgtcgagg gtcgcgatgt cctcgatgcg
     2581 ctgcgcgacg ctcaggtcga tgagccgcgg cggcgccgac atgacgacgt tcttcggctt
     2641 gacgtccaga tggacgaagc cgcgcgcggc catgtagtgg agcgccgagg tcagctcgag
     2701 cgcgagcggc aggagctgct cgaggatcac gccgtagcgg cggatgagcg tggagagccg
     2761 cggcccgtcc aggtactcga gcaggacgtg cggccggtcg ccgccgagca ccgcgccgaa
     2821 cccgcgcacg agcgagggat gggcgagcgc gtcgagcgcg gccgcctcgc gggcgagccc
     2881 gtggagcgcg cgaccgtcct cgacgaggtc ggggcgcagg agcttcgcga cgacgagcgc
     2941 gtgcagctcg tcgtcccacg tgaggtacgc ctcgtagcga cgcccgccgc cgagcaggtc
     3001 gacggcgtag cggccgggaa cgatctcgtc gccctcggcc agctcccagg actcgtacga
     3061 cggttccacg tccgtaggtg tatcccggtg cgatgaggcc ggaatgaggg caggatgaga
     3121 agattctcat gctcttctca tcattcgctc gcccgctcgg gtgacgatcg acccgatgac
     3181 gagcacggcc gagaccgaca ccggcggcaa gcccggcctg cgccggcgga tccgcgagcg
     3241 catcgcgggc ccgcgtgcgc ggctcctgcg gatgccgtcg agcatccgga cgcggacgat
     3301 cgtctggttc gtcggcgtcc tcgccgtcgc gacgctctcc tcggtgctcg tgacgcgcgg
     3361 cgtgctcctg atccggctcg accagcgcgt cgactcggag ctcacgcagg aggcggccga
     3421 gctgcgccgc ctcgccggcg gcaacgaccc ggcgaccggc gacccgttcc gcggagacgt
     3481 gaaccgcgtc ttccgcgtct tcctccagcg gaacatcccg gccccgtacg aggcgatcgt
     3541 gaccttcgtg gacggagagc cgttcctgcg ctcgcggccg gtgacagcgt accgcctcga
     3601 cgaggacccc gagatcgcgg accgctgggc gcggctcgag gaggccgagc gcgacgctct
     3661 ggacacgccg gagggccgcg tcgagtacct cgccgtgccg ctcgagttcg gcggcgagac
     3721 gcgcggcgtc ttcgtctcgg cgatcttccg cgaccgggcg aggacggagg tggacgaggc
     3781 gacgcgcgcg gccgcggcgg tcgggctcgc ggtgctcctg ctcggctcgc tcctcgcctg
     3841 gcgcctcgcc gaccggatcg tccggcccgt gaccgagctc acgcgcacgt cgcgcgcgat
     3901 cacggagacc gagttcagcc gccggatccc ggtcgaggga caggacgagg tggcccagct
     3961 cgcgacgacg tacaacgaga tgctcgaccg gctcgagagc gcgttcgcgt cgcagcggga
     4021 gttcctcgac gatgtcggcc acgagctgaa gacgccgctc acgatcgtcc gcggccacct
     4081 cgagctgctc gaggacgatc ccgaggagcg gcgggagacg ctcgcgctcg tcctcgacga
     4141 gctcgaccgg atggcgcgga tcgtcgagga cctcctcgtg ctcgcgaagc acgagcggcc
     4201 cggcttcctg aggctcgaga cggtcgacgt gggcgagctg acggacgcga tctacgcgaa
     4261 ggtcgccccg ctcgagtcgg ccgagtggat cctcgagaac cgcgggcgcg gcgtgatcgt
     4321 cgcggacggg cagcggctca cgcaggcggt gctccagctc gcgcagaacg cggtgcgcca
     4381 cgccggcggc acgatcgcgc tcggctccca ggtcgagaac ggcgaggcgc gcttctgggt
     4441 gcgcgaccag gggcccggca tcgacccgga ggagcagaag acgatcttct cccgcttccg
     4501 ccgcggcgag tcggccgggc gcgaggggtc ggggctcggc ctcacgatcg tccgggcgat
     4561 cgccgagcgc caccacggcc gcgtcgaggt ggcgagcatc cccgggatcg gctcgacctt
     4621 cgccgtcgtc gtccccgtcg accagccgca cgaggaggag atcgcatgag caggatcctg
     4681 atcgtcgagg acgaggcgcg gatcgcgtcg ttcgtggaga agggcctcaa ggcgaacggc
     4741 ttcgcgacgc gcgtcgcggg cgacggcgag tcggcgctcg ggctcgtccg cgcgggccag
     4801 tacgacctcg tgatcctcga catcgggctc ccggggatcg acggcttcca ggtgctcgag
     4861 gagctgcggg agaagcggcc cgcgctcccg gtcgtgatcc tgacggcgcg cgacagcgtc
     4921 ggggacaccg tggcggggct cgagggcggc gcggacgact acatcacgaa gcccttccgc
     4981 ttcgaggagc tgctcgcgcg cgtgcgcctg cggctgcgcg ggagccgtcc ggccgagccg
     5041 acggtgctcg aggccggcga cgtcgccctc gacctgcgca cgcgccaggc gatcgtggac
     5101 ggcgcgacgt acgacctgag cgcacgcgag ttcgcgctcg ccgaggtgtt cttccgccat
     5161 cccggccagg tgctgagccg ggagcagctc ctgagccagg tctggggcta cgacttcgac
     5221 ccgggctcga acgtcgtgga cgtgtacgtc ggctacctgc gcaagaagct cggcaagcgg
     5281 cgcatcgtca gcgttcgcgg catgggctac cgcttcgagc gcgagtagca cgcgcgtcgc
     5341 ctgcggaaaa gccgcaggcc gttcggcggt cgagccgcac cggttttcgg cgtccgcccc
     5401 gattgcgccg cgcccgtccg cgacgaagct gcgactcgtc aagcgagtga aagggcgagt
     5461 gacatgacca gcaagcgcaa gattctcgag atcggcgggg tcgccgccgg cgtcatcctc
     5521 atcgcgttcg ggatcgccgc gatcgtgatg ggctacaacg ggcggaccga ggttcgcgac
     5581 agcctcgccc gcgagaacat cgtcgggact cccgactcga ccattccggg ccagaaggtc
     5641 gacaccggct ccgaggccaa ggccttcgcc gacgtcatgc gggcccacac gctcgagatc
     5701 acgggcggcc agacgtacgc cgagatgggt cgcttcctcg acgaggacgg caacccgacg
     5761 aacgacgagg ccgcggccgc cgtcggcgac aacggccagc ccgtcgagaa cggcctgcgg
     5821 caggtctggg tgaccgagac cgcgctctcg acggcgctca acatgagcta catggccgag
     5881 cagctcgcgc tcttcggcat cgtggtcgga gtcgcgctcc tcttgagcgg catcggcttc
     5941 ctcgtcctcg ccctgggcgg cgcgctccgc caccgcgaga cggcggccgc cgcctccggg
     6001 acggccgccc gcccgctgac gacataagga agtcgcggaa gctcaggggc caacgggccc
     6061 ggcgaaagcc gggcccgtcg ctatttcggg gggagcgacg aggcgaacga ctcggcgcgc
     6121 gcgacccatg cgtcgaggcc ttcgtccgtc tccgtcgcgg ccgggccgac gaggacgaag
     6181 cccttcatcg gccggccggt gaagtcgaac ggccgcacgt gcggctcggc gagcgcggcc
     6241 tcaccgtcct ccgcggcgag gcgcacgacg agctcgtcgc ccagcacgcc gcagcacatg
     6301 ttgccgccga gcatgaagcc gatcccgccg aacatcttcc gctcggtcag gtccgggcgc
     6361 tcctcgagca gctcgcgcac ccgctcggcc aggccctcgt cgtacggcat ggcgcggagc
     6421 atggcatagg ctggcccgcg tgcgggcgct gctcctgccg gggatcgtca cgccggccgc
     6481 cgtggcctac gagcggctcg tgcccgcgct cgggccggag gtcgacgcgc gcccgaagga
     6541 gctcgaggtc tacgccggcc cggagccgcc gccgggctac tcgctcgagg tggagatgga
     6601 cggcgtcctg cgcgcggccg acgacgccgg ctgggagcgg ttccacctcg tcggctactc
     6661 gggcggcggt gcggtcgcgg cgtcgcttgc ggcctcgcgg ccggagcggc tcctgagcct
     6721 cggcctcctc gagcccgcct ggacggggaa cggcgggctg agcgagacgg aggcgaaggc
     6781 gcgcgccgag ttcgacgcgc tcgacggcct cgagccgcgg gagttccttg cgaccttcgc
     6841 ccgcctgcag ctccggcccg gcgtcgagcc gccgcccccg ccggacccgc cgccgtcgtg
     6901 gatggcgacg cgcccggccg cgatcgccgc gctgaccctc gcgtttcgca ctgacgagct
     6961 gtccgcggag tcgctgcgcc gcttcgaccg gcccgtctac cacgcgctcg gcggccgctc
     7021 ccgccccgag ctctacggcg agcagtcggc ccgcctcgcc cgcctcttcc ccgacttcac
     7081 ggaggaggtc ttcgaggagc gccaccactt cgacccgccc caccgcgccg agccggagcg
     7141 gctcgcggcc tcgctcctcg cccactggcg ccgcgcggag gcaggctgac taggtcgtcg
     7201 cagccacggg cggcgtgagc gagcgcgcgc gccagaggac gtaggcgacg ccggcgacga
     7261 ggatcgccgc catcacgacg agcagcgcga cgtcgtcgcc ggtcggcttc acgaccatgt
     7321 cgaggacgac tagcaagagc acgatgtagt cgatccgggc gagcgtgagg agcttgcggc
     7381 cctcgaacag cgcctcgggc gtcatct
//
LOCUS       DASTFC010000336         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_191736,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000336 DASTFC010000000
VERSION     DASTFC010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191736"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFO88_09885"
     CDS             <1..417
                     /locus_tag="VFO88_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545877.1"
                     /translation="LAFTGLWLLLAWLVPVMLTALEDVVAVAVGAAVLAVALASFFLS
                     LARLHRRMVEVKDREVGLARSLYAEAYEPVRSVRTLKELDRQRGLLAAADALEKRAHA
                     IHEWPIDERTVARVLTIATSVVGITIARLILDPFGL"
     gene            complement(489..1514)
                     /locus_tag="VFO88_09890"
     CDS             complement(489..1514)
                     /locus_tag="VFO88_09890"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET9545878.1"
                     /translation="MDIATLNETARALVAEGKGILAADESSGTIKKRFDSIGVESTEE
                     NRRGYRDLLFTTEGAEEHISGVILFDETIRQSSSDGTPFPRLLESKGIVPGIKVDTGA
                     KPLALADGETVTEGLDGLRERLAEYRELGARFAKWRATYSIGDGKPSEYCVWVNAHAL
                     ARYAALCQEAGLVPIVEPEVLQDGTHTIEESAKATGRVLQAVYTELHDQRVEYAGTLL
                     KPNMVLSGYEASTRAGAREVANATLECFYKHVPAAVPGIVFLSGGQTDEDATAHLNEL
                     NSLGPHPWQLSFSYGRALQAPALKAWGGSAENVEAAQRAYLHRARMNGLARSGAYSPE
                     MEEAVAA"
     gene            1955..2027
                     /locus_tag="VFO88_09895"
     tRNA            1955..2027
                     /locus_tag="VFO88_09895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1988..1990,aa:Gly,seq:ccc)
BASE COUNT          254 a          753 c          687 g          333 t
ORIGIN      
        1 ctcgccttca ccgggctctg gctcctgctc gcgtggctcg tcccggtcat gctcaccgcg
       61 ctcgaggacg tcgtcgcggt cgcggtcggc gcggccgtcc tcgccgtcgc cctcgcgagc
      121 ttcttcctct cgctcgcccg ccttcaccgg cgcatggtcg aggtgaagga ccgggaggtc
      181 gggctggcca ggagcctgta cgcggaggcg tacgagcccg tccgctcggt gcggacgctc
      241 aaggagctcg accggcagcg cggcctcctc gcggcggccg acgcgctcga gaagcgcgcg
      301 cacgcgatcc acgagtggcc gatcgacgag cgaacggtcg cgcgcgtcct cacgatcgcc
      361 acgagcgtcg tcgggatcac gatcgcgcgc ctcatcctcg acccgttcgg gctctagctc
      421 gcgcctggag tgcgcgggat cgggttcatc cggacggccg cggaagaagc ccgccccgcc
      481 cgccccgcct acgcggcgac cgcctcctcc atctccggcg agtaggcgcc gctccgcgcg
      541 agcccgttca tccgggcgcg gtggaggtag gcccgctgcg ccgcctcgac gttctccgcc
      601 gagccgcccc acgccttgag cgcgggcgcc tggagcgcgc ggccgtacga gaacgagagc
      661 tgccacgggt gcggcccgag cgagttcagc tcgttcagat gcgcggtcgc gtcctcgtcc
      721 gtctggccgc ccgagaggaa gacgatcccg ggcaccgccg ccggcacgtg cttgtagaag
      781 cactcgagcg tcgcgttcgc cacctcgcgc gcgcccgcgc gggtcgacgc ctcgtacccg
      841 gagagcacca tgttcggctt gaggagcgtt cccgcgtact cgacgcgctg gtcgtgcagc
      901 tccgtgtaga cggcctggag cacgcggccc gtcgccttcg cgctctcctc gatcgtgtgc
      961 gtcccgtcct ggagcacctc cggctcgacg atcgggacga gcccggcctc ctggcagagc
     1021 gccgcgtagc gcgcgagcgc gtgcgcgttc acccagacgc agtactcgct cggcttgccg
     1081 tcaccgatcg agtacgtcgc gcgccacttg gcgaagcgcg cgccgagctc gcggtactcc
     1141 gcgagccgct cgcgcagccc gtcgaggccc tcggtgaccg tctcgccgtc ggcgagcgcc
     1201 agcggcttcg cgcccgtgtc gaccttgatc ccgggcacga tccccttcga ctcgaggagc
     1261 cgcgggaacg gcgtcccgtc gctcgagctc tggcggatcg tctcatcgaa caggatgacc
     1321 ccgctgatgt gctcctccgc gccctcggtc gtgaagagga ggtcgcggta gccgcggcgg
     1381 ttctcctcgg tcgactcgac gccgatcgag tcgaagcgct tcttgatcgt ccccgagctc
     1441 tcgtcggcgg cgaggatccc cttgccctcg gcgacgagcg cgcgggctgt ctcgttgagc
     1501 gtggcgatgt ccatgacggc tccttccggc gcggttcgct gccgtcagta tcccccttcg
     1561 cggcgcgtcg atgcgtcgac ggtccacgcg gaaaggcccg gggtcttccc tccggggcca
     1621 agtgcgatta ctatcctgac aagacggagg gacggctctg cggcgggcgt ccctccgttg
     1681 tcttgtccgc tacgacaaga acgcccgcac gaagtcgagc cgggcagcga cccggtcgga
     1741 cagcacggcg agggcggcca ggatcgcggg cgtgatgacg gcgaggacga cggcgtactc
     1801 gctcatcgtc tgcccttcct cacgcctcag atctcggaaa accgctcgca tgcgtcacct
     1861 cctgttgtcc ccggcggccg cacgcccggt ttttctgccc tcattgtcgg gcggggccgg
     1921 ggattcgcct atccctcgct agaatgaccc gcacgcggac gtagctcagt tggtagagca
     1981 tcagcttccc aagctgaggg tcgcgggttc gagccccgtc gtccgct
//
LOCUS       DASTFC010000337         9554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_191855,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000337 DASTFC010000000
VERSION     DASTFC010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191855"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..193
                     /gene="rpsC"
                     /locus_tag="VFO88_09900"
                     /pseudo
     CDS             1..193
                     /gene="rpsC"
                     /locus_tag="VFO88_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="forms a complex with S10 and S14; binds the lower
                     part of the 30S subunit head and the mRNA in the complete
                     ribosome to position it for translation; incomplete;
                     partial in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            799..1224
                     /gene="rplP"
                     /locus_tag="VFO88_09905"
     CDS             799..1224
                     /gene="rplP"
                     /locus_tag="VFO88_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9545879.1"
                     /translation="MLMPRKTKFRKQQRGRRRGAAKGQQSVNFGDYGLKSLDAGWVTN
                     RQIEAARIAMTRKIKRGGKVWINVFPDKPVTQKPAETRMGSGKGSPEHWVAVVKPGRV
                     MFELAGVPEDLAREAMRLAGMKLPVKTKFVKREAELFEG"
     gene            1214..1417
                     /gene="rpmC"
                     /locus_tag="VFO88_09910"
     CDS             1214..1417
                     /gene="rpmC"
                     /locus_tag="VFO88_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9545880.1"
                     /translation="MKARELRELSDEELAKRLAETREELFNLRFQDATGALDNTARLG
                     LTKRDIARILGIQAERANRLERR"
     gene            1795..2073
                     /gene="rpsQ"
                     /locus_tag="VFO88_09915"
     CDS             1795..2073
                     /gene="rpsQ"
                     /locus_tag="VFO88_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9545881.1"
                     /translation="MVRLPKPEKSRAARKERRGVVVSNAMDKTIVVRVDSAKPDRRYK
                     KVVRRSKRFHAHDERNEANVGDLVRIVETRPLSRTKNWRLAEIVEAAK"
     gene            2076..2444
                     /gene="rplN"
                     /locus_tag="VFO88_09920"
     CDS             2076..2444
                     /gene="rplN"
                     /locus_tag="VFO88_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9545882.1"
                     /translation="MIQQESKLRVADNTGARSLLCIRVLGGSKRRYARVGDVIVGTVK
                     VATPQGTVKKGEVVKAVIVRTKKPFGRDDGTSIAFDENAAVIIDNQNNPRGTRIFGPV
                     ARELREKNFMKIVSLAPEVL"
     gene            2462..2815
                     /gene="rplX"
                     /locus_tag="VFO88_09925"
     CDS             2462..2815
                     /gene="rplX"
                     /locus_tag="VFO88_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9545883.1"
                     /translation="MRKGDLVQVISGKDRGKQGRIIEALPRENRVIVENLNVVKKHQR
                     PRAMRDSSRMGQQQIQPGGVFDVSAPMPASNVMVVCPVCNRATRVGYEFRDSKDGQVK
                     VRVCKRADCGEVLDR"
     gene            2845..3381
                     /gene="rplE"
                     /locus_tag="VFO88_09930"
     CDS             2845..3381
                     /gene="rplE"
                     /locus_tag="VFO88_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9545884.1"
                     /translation="MKARYEDELRASLKDELGFSSIMQVPRVEKITLNMGVGEAKTNA
                     KALDDAIEELTLIAGQRAQVRRATKSIAGFKIREGMPIGTKVTLRGARMYEFLDRLVS
                     IALPRIRDFRGLSPRSLDGRGNYSLGIREQIIFPEIDYDNVATIRGLDVAITTTAKTD
                     EEALALLRGLGLPFRTDA"
     gene            3397..3582
                     /locus_tag="VFO88_09935"
     CDS             3397..3582
                     /locus_tag="VFO88_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET9545885.1"
                     /translation="MAKKSLIVKASRPKKFSTREYSRCNRCGRPRAVYRKFGLCRICL
                     RELAHDGAIPGMTKSSW"
     gene            3590..3985
                     /gene="rpsH"
                     /locus_tag="VFO88_09940"
     CDS             3590..3985
                     /gene="rpsH"
                     /locus_tag="VFO88_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9545886.1"
                     /translation="MMTDPVADMLTRIRNANKALQETVSMPTSRMKEEIARLLAEEGY
                     IRGYTVEKGEAFDTLVVELKFGQNRERVITDLKRVSKPGRRVYARKDRLPRVLGGMGT
                     AILSTSTGLVTSRRAQELGIGGEVVCFVW"
     gene            3993..4526
                     /gene="rplF"
                     /locus_tag="VFO88_09945"
     CDS             3993..4526
                     /gene="rplF"
                     /locus_tag="VFO88_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9545887.1"
                     /translation="MSRIGKRPIELPTGVSVAISPGRVMVNGPLGELSQGIPQRISVE
                     QGDGELLVKRPTERGEDRALHGLARTLVANMVEGVTKGFEKRLEIQGVGYRAALRGTA
                     VELNVGYSHPVVKDAPDGITFEVPTPTEIVVKGIDKQQVGQVAAEIRKVRPPEPYKGK
                     GIRYQGEYVRRKVGKRA"
     gene            4526..4882
                     /gene="rplR"
                     /locus_tag="VFO88_09950"
     CDS             4526..4882
                     /gene="rplR"
                     /locus_tag="VFO88_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9545888.1"
                     /translation="MPTLSTREARLRRHRRLRRTISGTAERPRLVVFRSNRGIEAQLV
                     DDVAGRTVAAASHTKLKKSFKGSKTEQAEAVGKLLAEQAKKEGVESVVFDRGGFLYHG
                     RVKALADGAREGGLRF"
     gene            4912..5394
                     /gene="rpsE"
                     /locus_tag="VFO88_09955"
                     /pseudo
     CDS             4912..5394
                     /gene="rpsE"
                     /locus_tag="VFO88_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293490.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            5513..5695
                     /gene="rpmD"
                     /locus_tag="VFO88_09960"
     CDS             5513..5695
                     /gene="rpmD"
                     /locus_tag="VFO88_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET9545889.1"
                     /translation="MAKTLRITQVRSQIGESQRHRGTLRALGLGRIGRTVERPESPET
                     AGMLRKVRHLVKVEES"
     gene            5698..6192
                     /gene="rplO"
                     /locus_tag="VFO88_09965"
                     /pseudo
     CDS             5698..6192
                     /gene="rplO"
                     /locus_tag="VFO88_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            6316..7569
                     /gene="secY"
                     /locus_tag="VFO88_09970"
     CDS             6316..7569
                     /gene="secY"
                     /locus_tag="VFO88_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9545890.1"
                     /translation="MLSWLTNAWRVPELRRRVLFTAFILALYRLGSWIPTPGVDSQAV
                     QDYFAGQGGTILNLLNIFSGGALSQFALFALGIMPYITASIILQLMTVVIPRLEQLQK
                     EGESGYAKINQYTRYLTVALAALQAAGYSYLFDRQGALTLDFPRFILIVISLTAATTL
                     LMWMGELITKRGIGNGISLLIFASILASLPGGIRAWYEGDAFERLLFPLIALAIIVAV
                     VFVQEGQRRIPIQYAKRMVGRRMTTGGTTYMPLRVNMAGVIPIIFAAAIMAFPPTVAQ
                     FFPSTQAFINEHFTPTDVVYVVLQGFLIVVFTYFYTAVQFNPVDQADNLRKHGGYIPG
                     IRPGPPTAQYLDRVLTRLTLPGSLYLALVATLPTLFIRYGGFSQSTSAALGGTSILIV
                     VGVALDTMRQMESQMMMRHYEGFLK"
     gene            7575..8150
                     /locus_tag="VFO88_09975"
     CDS             7575..8150
                     /locus_tag="VFO88_09975"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9545891.1"
                     /translation="MPLDIVILGPPGAGKGTQAAKIEADAGIPHIATGDIVRAEMAAE
                     TPFGLKVMEYNLRGELVPDELMIERVRERLLEGDTEDGFVLDGFPRTLAQAQALDGML
                     DELDRSIRVVLHFQLPEELAEQRLLGRALASGRADDTPEVIHKRMATMRVPHDLIAYY
                     RALGNLVGIHADRSIEEVYAEVQNVLETAAA"
     gene            8147..8914
                     /gene="map"
                     /locus_tag="VFO88_09980"
     CDS             8147..8914
                     /gene="map"
                     /locus_tag="VFO88_09980"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9545892.1"
                     /translation="MIVRKSADEIETMARAGRVVAETIALMGETIRPGISTRELDELA
                     EELIRSRGGVPTFKGYRGFPASICASPNDMVVHGIPGGYRVEEGDLLTVDVGVTLEGF
                     VADSAYTFAVGEVGEEAERLLAVCQAALAAGIAEARDGNHVGDISAAVQRVTEEAGFS
                     VIKSLVGHGVGRSMHEEPQVPNYGRPGRGPRLATGMTIAIEPMITAGGEEVAVADDDW
                     SISTVDGSLSAHFEHTVAVTENGPRILTEAPVTSLVP"
     gene            8964..9077
                     /gene="rpmJ"
                     /locus_tag="VFO88_09985"
     CDS             8964..9077
                     /gene="rpmJ"
                     /locus_tag="VFO88_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9545893.1"
                     /translation="MKVRPSVKPMCDRCRVIKRHGRVLVICTNPRHKQRQG"
     gene            9087..9461
                     /gene="rpsM"
                     /locus_tag="VFO88_09990"
     CDS             9087..9461
                     /gene="rpsM"
                     /locus_tag="VFO88_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9545894.1"
                     /translation="MARIAGVNLPREKRLEVALTYIYGIGPSTARKVATDLGLTPDEK
                     VRDLTDEEITKLRQYIDGELQVEGDLRRERTQNVKRLQEIGAYRGVRHRRGLPVNGQR
                     TKTNARTRKGPKKTVGRGKKKG"
BASE COUNT         1646 a         3271 c         3459 g         1178 t
ORIGIN      
        1 cgcccagggc gtccgcgtcc agtgcggcgg ccggctcggc ggcggcgaga tgtcccgctc
       61 cgagagctac tccgagggcc gcgtcccgct gcacacgctg cgcgcggaca tcgactacgg
      121 cttcgccgag gcgaagacga ccttcggccg gatcggcgtc aaggtctgga tcaacaaggg
      181 cgagatcatg ccggagggct acggcgacgg cggcgacggc gagacgtcgc ggcggcgcac
      241 gaacctcgag ggcctgagcg ccggccgcga gggcggccgc cgcgcgcccg accgcgaggg
      301 tctcggcccg gtccggcggc gcggctccgg ccgcgggcgc ggcggcggcg ggcggcgcgg
      361 tccgggcggc cagggcggtc ccggcggcca gacggagcgc ccgcgcaccg acgcgcgcgg
      421 cgtctcggcc gaggggcgcg agcagccgaa ggtcaccccc gaggtcacgg aggagccgaa
      481 accggtggcc gaggcgccgg acacgacgac ggccaagacg atgcacgagg acccgacgaa
      541 gctcgagcag tcgggcgacc ccgcggccga gaagccgaag cgctccggcg cgaagacgtc
      601 cgcgaagaag acggagtcgg gcgagagcgc cggccaggcc ccgaaggcgg aggccggcac
      661 ggccgaggcg cccgcggagg agaagccgaa ggcgacccgc tcgaaggccg cctcgaagcc
      721 gaaggccgac gaggccccgg ccgaggagaa gccgaagcgc accacccgca ggaagaagga
      781 cgaggggggt gacgcctgat gctgatgccg cggaagacga agttccgcaa gcagcagcgc
      841 ggccgccggc gcggcgccgc gaagggtcag cagagcgtga acttcggcga ctacgggctc
      901 aagtccctcg acgcgggctg ggtgaccaac cgccagatcg aggcggctcg tatcgccatg
      961 accaggaaga tcaagcgcgg cgggaaggtc tggatcaacg tcttcccgga caagcccgtc
     1021 acgcagaagc cggccgagac gcgcatgggc tccggcaagg ggtcgcccga gcactgggtg
     1081 gccgtcgtga agcccggacg cgtgatgttc gagctggccg gcgtgcccga ggacctcgcg
     1141 cgcgaggcga tgcggctcgc cgggatgaag ctgcccgtca agacgaagtt cgtgaagaga
     1201 gaggctgagc tctttgaagg ctagggagtt gcgagagctg tccgacgagg agctcgcgaa
     1261 gcgcctcgcc gagacccgtg aggagttgtt caacctccgc ttccaggacg cgaccggcgc
     1321 gctcgacaac accgcgcgcc tcggtctcac gaagcgggac atcgcccgga tcctgggcat
     1381 ccaggccgag cgtgcgaaca ggctggagag aaggtaatgg ctgacgacga gaagaacgag
     1441 cagaccgagc ccgagacgac cgacgaggcc gtcgaggagc ccgtggccga ggagccggcg
     1501 gccgaggagc ccgtcgcgga ggaggctgcc gcggaggggg cgcccgccga ggagcccgtg
     1561 gccgaagagc ccgtggccga ggaggaggcc gcgccggagg ccgaggccgc acccgcggag
     1621 gagcagccgg ccgtcgacgc cgaagccgcg ccggaggcca agcccgagcc cgccgccacg
     1681 cccgcgcggg agaagcgccg ctcgacgaag acgaaggccg agaagcgcgc gcagcgcgag
     1741 gccgtgaagg agcgccgcaa ggccgccccg cgtcccgagc gcgagcgcaa gccgatcgtc
     1801 cggctcccga agccggagaa gtcgcgcgcg gcccgcaagg agcgtcgcgg cgtcgtggtc
     1861 tcgaatgcga tggacaagac gatcgtcgtc cgcgtcgaca gcgcgaagcc cgaccggagg
     1921 tacaagaagg tcgtccggcg ctcgaagcgg ttccacgcgc acgacgagcg caacgaggcg
     1981 aacgtgggcg atctcgtccg catcgtcgag acgcgcccgc tctcgcgcac gaagaactgg
     2041 cgcctggccg agatcgtgga ggcggccaag tagcgatgat ccagcaggaa tccaagctca
     2101 gagtcgccga caacacgggc gcccgctcgc ttctctgcat ccgcgtgctc ggcggctcga
     2161 agcgccgcta cgcgcgggtc ggcgacgtga tcgtcggcac cgtcaaggtc gcgacgccgc
     2221 agggcacggt gaagaagggc gaggtcgtga aggcggtcat cgtccgcacg aagaagccct
     2281 tcggccgcga cgacggcacc tcgatcgcgt tcgacgagaa cgccgcagtg atcatcgaca
     2341 accagaacaa cccgcggggg acgcgcatct tcggcccggt ggcgcgcgag ctgcgcgaga
     2401 agaacttcat gaagatcgtc tccctcgcac cggaggtgct gtagtggcga ccaagctgaa
     2461 gatgcgcaag ggcgacctcg tccaggtcat ctcgggcaag gaccgcggga agcagggccg
     2521 gatcatcgag gccctccctc gcgagaaccg ggtgatcgtc gagaacctga acgtcgtgaa
     2581 gaagcaccag cggccgcgcg cgatgcgcga ctcgagccgc atgggccagc agcagatcca
     2641 gccgggcggc gtgttcgacg tgagcgcgcc gatgcccgcc tcgaacgtca tggtcgtctg
     2701 cccggtctgc aaccgcgcga cccgggtcgg ctacgagttc cgcgactcga aggacggcca
     2761 ggtgaaggtc cgcgtctgca agcgcgccga ctgcggggag gtgctcgaca ggtgagcccg
     2821 accgcgaccg agtacgtgcc gcgcctgaag gcgcgctacg aggacgagct ccgcgcgagc
     2881 ctgaaggacg agctgggctt cagctcgatc atgcaggtcc cgcgcgtcga gaagatcacg
     2941 ctgaacatgg gcgtcggcga ggcgaagacg aacgcgaagg cgctcgacga cgcgatcgag
     3001 gagctcacgc tcatcgccgg ccagcgggcg caggtccgcc gggcgacgaa gtcgatcgcg
     3061 ggcttcaaga tccgcgaggg catgccgatc ggcacgaagg tcacgctccg cggcgcgcgg
     3121 atgtacgagt tcctcgaccg gctcgtctcg atcgcgctcc cgcgcatccg cgacttccgc
     3181 gggctctcgc cgcggagcct cgacgggcgc ggcaactact cgctcggcat ccgcgagcag
     3241 atcatctttc cggagatcga ctacgacaac gtcgccacga tccgtggcct ggacgtggcg
     3301 atcacgacca cggcgaagac cgacgaggag gcgctcgcgc ttctgcgcgg gctcggtctc
     3361 ccgttccgca cggacgcgta ggaggcagga ggaaccttgg ccaagaagag cctgatcgtc
     3421 aaagcgtcgc gaccgaagaa gttctcgacg cgcgagtaca gccggtgcaa ccgctgcggg
     3481 cgtccccgcg cggtgtaccg gaagttcggc ctctgccgga tctgcctgcg cgagctcgcg
     3541 cacgacggcg cgatccccgg catgacgaag tcgagttggt gatgcggcga tgatgaccga
     3601 ccccgtagcc gacatgctca cccggatccg caacgccaac aaggcgctgc aggagaccgt
     3661 ctcgatgccg acctcccgca tgaaggagga gatcgcgcgc ctgctcgcgg aggagggcta
     3721 catccgcggg tacacggtgg agaagggcga ggcgttcgat accctggtcg tcgagctgaa
     3781 gttcgggcag aaccgcgagc gggtcatcac cgacctgaag cgcgtctcga agcccggccg
     3841 ccgtgtctac gcgcgcaagg accgcctgcc gcgcgtgctc gggggcatgg gcacggcgat
     3901 cctgtcgacc tcgacgggcc tcgtcacgag ccgccgcgcg caggagctcg gaatcggcgg
     3961 agaagtcgtc tgcttcgtgt ggtgagcctg agatgagccg gattggaaag agaccgatcg
     4021 agctgcccac cggggtgagc gtcgcgatct cgcccggccg cgtcatggtg aacgggccgc
     4081 tcggcgagct ctcgcagggg atcccgcagc ggatctcggt cgagcagggc gacggcgagc
     4141 tcctcgtgaa gcgcccgacg gagcgcgggg aggaccgcgc gctccacggc ctcgcgcgga
     4201 cgctcgtcgc gaacatggtc gaaggcgtca cgaagggctt cgagaagcgc ctggagattc
     4261 agggcgtcgg ctaccgcgcg gcgctgcgcg ggacggcggt cgagctgaac gtcggctact
     4321 cccacccggt cgtgaaggac gcgcccgacg gcatcacctt cgaggttccg accccgaccg
     4381 agatcgtcgt gaagggcatc gacaagcagc aggtcggcca ggtcgccgcc gagatccgca
     4441 aggtccgccc gccggagccg tacaagggca agggcatccg ctaccagggc gagtacgtcc
     4501 ggcggaaggt cgggaagcgg gcctgatgcc gacgctctcg acacgcgaag cgcgcctgcg
     4561 ccggcatcgc cggctgcggc gcacgatctc cggcaccgcc gagcggccgc gcctcgtagt
     4621 cttccgctcg aaccggggaa tcgaggcaca gctcgtcgac gacgtggcgg gccgcacggt
     4681 cgccgccgcg agccacacga agctcaagaa gtcgttcaag ggctcgaaga cggagcaggc
     4741 cgaggccgtg ggcaagctcc tcgccgagca ggcgaagaag gagggcgtcg agtcggtcgt
     4801 gttcgaccgc ggcggcttcc tctaccacgg ccgggtcaag gcgctcgccg acggcgcgcg
     4861 cgaaggagga ttgcgatttt gagcaccgtc gagcgtcagg tggagcgccg gatgatggcc
     4921 gagcgcgagc tgaaggagcg cgtggtcgag atcaaccgcg tcgccaaggt cgtgaagggc
     4981 ggccggcggt tctcgttcac cgcgctcgtc gtgatcggcg acgaggtcga ccgcgtcggc
     5041 gtcgggtacg ggaaggcgcg ggaggtcccg ctcgccatct cgaaggccgt cgacgacgcg
     5101 aagaagaacc tcttcgaggt gccgaagcac cacacgacga tcacgcacga gatcatcggc
     5161 gagttcgacg ccgcgcgggt gctcctgcgc ccggcgagcg agggaaccgg cgtgatcgcc
     5221 ggcggcggcg tccgcgccgt cctcgagctg gccgggatcc gcgacatcct cgccaagagc
     5281 ctcggcacgc cgacgccgat caacatgctg aaggcgacgg tgaacggcct gcagaacctg
     5341 cgccggcccg aggacgtcgc tcggatccgc gggaagacga tcgccgaggt gctcccctgg
     5401 aagccgccgg cggagccgga ggccgaggag gcgcccgccc cggaggcggc cgcggcggcc
     5461 gagggggccg cggcggccga ggagcccgcc ggggaggcga cggaggagac gcgtggcgaa
     5521 gacgcttagg atcacgcagg tccggagcca gatcggcgag tcgcagcgcc atcgcggcac
     5581 gctccgcgcg ctcggcctcg gccggatcgg ccgcaccgtc gagcgtccgg agagcccgga
     5641 gacggccggt atgctccgca aggttcgcca cctcgtgaag gtcgaggagt cctagccatg
     5701 ccgcccgaga tcgcacccga cgaggagctc tcgctccaca cgctgaagcc cgcccagccg
     5761 cgcaaggcgc gcaagcggat cggccgtggc ctgggctccg gcaagggccg ctacagcggc
     5821 cgcgggatca agggccagaa ggcgcgctcc ggctcgcaca cgatgcggcc ggggttcgag
     5881 ggcggccaga tgccgatcta catgcggctc ggcaagcagc gcggcccgta ctcgaaggac
     5941 gcgatgcccg tcggcccgca ccggacgcag accgtgccgg tgaacctccg cgacctcgac
     6001 cgcgtcttcg acgacggtgc cgaggtgacg ctcgaggcga tgatcgagaa gggcctcgtg
     6061 aagagctcgc gcatcgacgt caagatcctc ggccagggcg agctcaagaa gaagctctcc
     6121 gtgaccgcgc acgcgttctc cgcgagcgcc cgcgagaaga tcgaggcggc cggcggctcc
     6181 gtgacggcgc tgcgggagcc ggccgagaag aagaagcgcc cccgcaaggc caagccggcc
     6241 gaggcggccc aggtcgagcc cgaggcggag gccggagagc ccgagcccga ggccgccgag
     6301 agcgaggagt agcgcttgct gtcctggctc accaacgcct ggcgcgtccc ggagctgcgc
     6361 cggcgcgtcc tcttcaccgc cttcatcctg gcgctctacc ggctcggaag ctggatcccg
     6421 acgccgggcg tcgactcgca ggccgtccag gactacttcg ccgggcaggg cggcacgatc
     6481 ctcaacctgc tcaacatctt ctcgggcggc gcgctcagcc agttcgcgct cttcgcgctc
     6541 gggatcatgc cgtacatcac ggcctcgatc atcttgcagc tcatgacggt cgtgatcccg
     6601 cggctcgagc agctccagaa ggagggcgag tccggctacg cgaagatcaa ccagtacacg
     6661 cgctacctga ccgtcgcgct cgcggcgctc caggcggccg ggtactccta cctcttcgac
     6721 cgccagggcg cgctcacgct cgacttcccg cgcttcatcc tcatcgtcat ctcgctgacc
     6781 gcggcgacga cgctcctcat gtggatgggc gagctcatca cgaagcgcgg gatcgggaac
     6841 gggatctcgc tcctcatctt cgcctcgatc ctcgcgagcc tccccggcgg gatccgcgcc
     6901 tggtacgagg gcgacgcgtt cgagcgcctg ctcttcccgc tcatcgcgct cgcgatcatc
     6961 gtggcggtcg tcttcgtcca ggagggccag cggcgcatcc ccatccagta cgcgaaacgc
     7021 atggtgggga gacgtatgac caccggcggg acgacctaca tgccgctgcg cgtgaacatg
     7081 gcgggcgtca tcccgatcat cttcgcggcc gcgatcatgg ccttcccgcc gaccgtcgcc
     7141 cagttcttcc cgtcgacgca ggcgttcatc aacgagcact tcacgccgac ggacgtcgtc
     7201 tacgtggtcc tgcagggctt cctgatcgtc gtcttcacgt acttctacac ggccgtgcag
     7261 ttcaaccccg tcgaccaggc ggacaacctg cgcaagcacg gcggctacat cccggggatc
     7321 cgtccggggc cgccgaccgc gcagtacctc gaccgcgtgc tgacgcggct gacgctgccc
     7381 ggttcgctct atctcgcgct cgtcgcgacg ctgccgacgc tcttcatccg ctacggcggg
     7441 ttctcgcagt cgacgtcggc ggcgctcggc ggcacgtcga tcctgatcgt ggtcggcgtc
     7501 gcgctcgaca cgatgcgcca gatggagtcg cagatgatga tgcggcacta cgagggcttc
     7561 ctgaagtagg gcgcatgccg ctcgacatcg tcatcctcgg cccgcccggg gcgggcaagg
     7621 gcacccaggc cgcgaagatc gaggcggacg cggggatccc gcacatcgcg accggcgaca
     7681 tcgtccgcgc cgagatggcg gcggagacgc cgttcgggct caaggtcatg gagtacaacc
     7741 tccggggtga gctcgtcccg gacgagctca tgatcgagcg cgtccgcgag cggctcctcg
     7801 agggcgacac cgaggacgga ttcgtgctcg acggcttccc gcgcacgctc gcccaggcac
     7861 aggcgctcga cgggatgctc gacgagctcg accgctcgat ccgcgtcgtc ctccacttcc
     7921 agctccccga ggagctcgcc gagcagcggc tgctcggccg cgcgctcgcg tccggccgcg
     7981 cggacgacac gcccgaggtg atccacaagc gcatggcgac catgcgcgtc ccgcacgacc
     8041 tgatcgcgta ctaccgcgcc ctcgggaacc tcgtcggcat ccacgccgac cgctcgatcg
     8101 aggaggtcta cgccgaggtg cagaacgtcc tggagaccgc ggccgcgtga tcgtccgcaa
     8161 gtccgcagac gagatcgaga cgatggcccg cgcggggcgc gtcgtcgcgg agacgatcgc
     8221 gctcatgggc gagacgatcc ggcccgggat ctcgacgcgc gagctcgacg agctcgccga
     8281 ggagctcatc cgctcccgcg gcggcgtccc gaccttcaag ggctaccgcg gcttcccggc
     8341 ctcgatctgc gcctcgccga acgacatggt cgtccacggc atcccgggcg gctaccgggt
     8401 ggaggagggc gacctcctga ccgtggacgt cggcgtgacg ctcgagggct tcgtcgcgga
     8461 ctcggcgtac acgttcgcgg tcggcgaggt tggcgaggag gccgagcgcc tgctcgcggt
     8521 ctgccaggcg gcgctcgcag ccgggatcgc cgaggcgcgg gacggcaacc acgtcggcga
     8581 catctccgcg gcggtgcagc gcgtgaccga ggaggccggg ttcagcgtga tcaagtcgct
     8641 cgtcgggcac ggggtcggcc gctcgatgca cgaggagccg caggtgccga actacggccg
     8701 cccggggcgc ggaccgaggc ttgcgaccgg catgacgatc gcgatcgagc cgatgatcac
     8761 cgcgggcggg gaggaggtcg ccgtggcgga cgacgactgg tcgatctcga ctgtcgacgg
     8821 ctcgctctcc gcccacttcg agcacaccgt ggccgtgacg gagaacggcc cccggatcct
     8881 cacggaggcg cccgtcacgt ctctggtacc ataggcgggc gcggtccgcc gggtctcggc
     8941 gtggccgctg tctctacgtc ctcatgaaag ttcgtccatc cgtcaagccc atgtgcgacc
     9001 gctgccgcgt catcaagcgg cacggacgcg tcctggtcat ctgcacgaat cctcgccaca
     9061 agcagcggca ggggtaagga gaagaattgg ctcgtatcgc aggcgtgaac cttccgcgcg
     9121 agaagcgcct cgaggtggcg ctcacgtaca tctacgggat cggcccgtcc accgcccgca
     9181 aggtggcgac ggacctcggc ctgacccccg acgagaaggt ccgtgacctg acggacgagg
     9241 agatcacgaa gctccgccag tacatcgacg gcgagctgca ggtcgagggc gacctgcgcc
     9301 gcgagcggac gcagaacgtc aagcgcctcc aggagatcgg cgcctaccgg ggcgtccggc
     9361 accgccgcgg cctcccggtg aacgggcagc ggacgaagac gaacgcgcgc acgcgcaagg
     9421 gcccgaagaa gacggtcggc cgcggaaaga agaaggggta accgctagat ggctcctccc
     9481 cgcagggcct ccaagggccg cacgcgccgc cgcgtgaaga agaacatcgc gatcggccag
     9541 gcgcacatca agac
//
LOCUS       DASTFC010000338         6072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_356473,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000338 DASTFC010000000
VERSION     DASTFC010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356473"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFO88_09995"
     CDS             <1..545
                     /locus_tag="VFO88_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET9545895.1"
                     /translation="GIRYDRLEEVGLQWPCLDEDDPGTAYLHAPRPARPSGRGKFYPV
                     EYQPPIEEPDSEYPFLLSTGRTLYHYNSATMTMRESGVTDKQEDPFVEISPEDASALG
                     VADGDWLRLTSRRGSIEARASVGDRVYPGLVWMALHFEEARVNLLTNDVTDSLIGIPE
                     YKVCAIKVEPVAPALRPAAR"
     gene            713..2212
                     /locus_tag="VFO88_10000"
     CDS             713..2212
                     /locus_tag="VFO88_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9545896.1"
                     /translation="MDASPDLDPRRAPFELDPDEFRRLGHETVDAIADFLRTLPERPV
                     TTSESPTEIRAALGGGGLPERGAPPDELLAEAARLLFDHSALNGHPRFFAYITSSAAP
                     IGALGDLLAAAVNPNVAGFPLAPLATELEAQTIRWIAELLGYPTDCGGLLVSGGNMAN
                     FVGLLAARAAQTTWDVRAHGLAAGEAAPLRVYVSSETHTWIQKAADLFGLGTDAIRWI
                     ETDGEQRMLVSALREQIAADRADGALPLLVVGTAGTVSTGAVDPLDELADLCRHEGIW
                     FHVDGAYGAPAAVLPDAPPDLAALARADSIAVDPHKWLYAPLEAGCVLVRDREKLRAA
                     FTYRPPYYRFEEGADAPIQYVDFGPQNSRGFRALKVWLVLRQAGREGYARAIAQDVEL
                     ARELHRLAEADPELEAWTTRLSITTFRYVPRGVEAGTPAGEELANRVNEELLGRLQSG
                     GEVFLSNAVVRGAYLLRACVVNFRTGLRDVRAIPEIVKRVGAEVAAELR"
     gene            complement(2365..3453)
                     /locus_tag="VFO88_10005"
     CDS             complement(2365..3453)
                     /locus_tag="VFO88_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9545897.1"
                     /translation="MDGVRAAVFEGPGRVSVEELELEAPRAREVLVRLGASGVCHSDL
                     HVVDGEWPRPTPVVMGHEGAGVVEAVGDGVRGVAVGDHVVLSWFYACGRCAHCLAGRA
                     WACSGTRAGQCVMDDGTTRFRRPGSGEDVFTYLAVGSMAERSVVPEAAAIPVPREVPW
                     EVAALIGCAVTTGVGAVWNTARVEPGASVVVVGCGGVGLSVVMGAALAGAHPIVAVDV
                     SEEKLALASSAGASHAVGTLDEARVLVAPGGADYVFEAIGLVETVEAVPSLLRSGGVG
                     VLVGLTAAGVRASFDVYSFVDAGKTLVGSIYGGAMPALDFPRIAELYLAGKLPLDRLV
                     SHRIELEGVEEAFEAMRRRERARSVVVF"
     gene            3588..5183
                     /locus_tag="VFO88_10010"
     CDS             3588..5183
                     /locus_tag="VFO88_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9545898.1"
                     /translation="MDEVLEAVVALLSDASAVHATFVYFLEDGGERLVLKAASDPYGR
                     FVDRIALERGEGLAWWAAERREPGFILDNALDDPRFKHVPELEEERFQSLLSVPILGK
                     DGGVIGVITLHTEAPREFTEPEIDFLVSSASLVAGAIENARLYEEMRRRVDELEHLTE
                     LGETITRAETVEELLPAVAERSLSLLRARSCHLYLLDAGSEELHLRASAPPGAEAPAS
                     VGLAGLGPELARSGRSASVAVPLVANDELLGLLRAEGTSAVDLARAAANQTAVALKKI
                     ELIERLTEKNRIRDFFDSLARGRIGPGVEGRAGRLGIDLTAPHLVLVASPPDDALEKA
                     LARAAPGSLLDRRDDALRGLLRIPHAGEETLVAAVRDAERAPETAVGLSNPCRGAESF
                     AAGFEEATHALLGSSVVRGEPAVMTYEELGPYKYLLRMSFETGMRDRHREAVSRLADY
                     DRRRSTALLKTLEEFLRRRGNISATAEALYIHPNTLRQRLRRIGELTGIDLRRDDWLM
                     VEIAVKIAKLEEVAGDTIRRDLM"
     gene            5258..>6072
                     /locus_tag="VFO88_10015"
     CDS             5258..>6072
                     /locus_tag="VFO88_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545899.1"
                     /translation="MAVAAETPTLDDIRKDVADGVEFFFAQFVDMHARPSAKLVPAGN
                     LDDLLSEGAGFAGFAAGDIGQVPADPDIAAIPDLRSYTRVPWEPSVARFACDIHVEGE
                     EWPYDPRTILRRQLERAREKGFEFRMGLELEYFLVELHEDGSITVADELDTLEKPCYD
                     LNGLSRHYEFLTTVSRYVNALGWGNYANDHEDANGQFEQNFTYADALTSCDRAIFFRY
                     MVHQLAEKWGRQVGRRLLATFMPKPFSHLTGNGCHFHMSLWDGDTNLFLDEDDP"
BASE COUNT          874 a         2335 c         2078 g          785 t
ORIGIN      
        1 gcgggatccg ctacgacagg ctcgaggagg tcgggctcca gtggccgtgc ctcgacgagg
       61 acgaccccgg caccgcgtac ctccacgcgc cgcggccggc ccgcccgagc gggcgcggga
      121 agttctaccc cgtcgagtac cagccgccga tcgaggagcc ggactccgag tacccgttcc
      181 tcctctcgac cggccggacg ctctaccact acaactcggc gaccatgacc atgcgcgaga
      241 gcggcgtgac cgacaagcag gaggatccgt tcgtcgagat ctcgccggag gacgcatccg
      301 cgctcggggt cgcggacggc gactggctgc gactcacgtc acggcgaggc tcgatcgagg
      361 cgcgcgcgtc ggtcggcgac cgcgtctatc cggggctcgt ctggatggcg ctccacttcg
      421 aggaggcgcg cgtcaacctc ctgacgaacg acgtgaccga ctcgctcatc gggatcccgg
      481 agtacaaggt ctgcgcgatc aaggtggagc cggtcgcgcc ggcgctgcgg cctgcggcgc
      541 gctagcgcta ccaccgcgcc ggggacgaat cggcacgaat tcgtcacatg ttcgcgcgcc
      601 tcacgtacgc ttttcggcga taggctcgag ccggtaggcg ggcgggtcgg gacccggttt
      661 agacgtgcgc gtctaacttt cccggccgcg gcccgcgggc tagagtcgcc gcgtggacgc
      721 gtcccctgac ctcgatccac gtcgcgcccc gttcgagctc gaccccgacg agttccggcg
      781 gctcggccac gagacggtcg acgcgatcgc ggacttcctc cgcacgcttc ccgagcggcc
      841 ggtcacgacg agcgagtcgc cgaccgagat ccgcgccgcg ctcggcggcg gtgggctgcc
      901 ggagcgcggc gcccctcccg acgagctcct cgccgaagcc gcgcgcctgc tcttcgacca
      961 ctccgcgctc aacggccacc cacgcttctt cgcgtacatc acgtcgtccg cggccccgat
     1021 cggcgcgctc ggcgacctcc tcgccgcggc cgtgaacccg aacgtcgccg gcttccccct
     1081 cgcgccgctc gcgaccgagc tcgaggcgca gacgatccgc tggatcgcgg agctgctcgg
     1141 atacccgacc gactgcggcg gcctcctcgt gagcggcggg aacatggcga acttcgtcgg
     1201 cctgctcgcc gcgcgcgccg cccagacgac ctgggacgtg cgcgcccacg ggctggcggc
     1261 gggagaggcc gcgccgctgc gcgtctacgt ctcctccgag acccacacgt ggatccagaa
     1321 ggcggccgac ctcttcgggc tcgggacgga cgcgatccgc tggatcgaga cggacggcga
     1381 gcagcggatg ctcgtctccg cgctccgcga gcagatcgcc gccgaccgcg cggacggcgc
     1441 gctcccgctg ctcgtcgtcg gcacggcggg aaccgtgagc accggcgcgg tcgacccgct
     1501 cgacgagctc gccgacctct gccgccatga ggggatttgg ttccacgtcg acggcgcgta
     1561 cggcgcgccg gccgccgtcc tgccggacgc gccgccggac ctggcggccc tcgcgcgcgc
     1621 ggactcgatc gccgtcgacc cgcacaagtg gctctacgcg ccgctcgagg cgggatgcgt
     1681 gctcgttcgc gaccgggaga agctccgcgc cgcattcacg taccggccgc cctactaccg
     1741 cttcgaggag ggcgcggacg cgccgatcca gtacgtcgac ttcggcccgc agaactcgcg
     1801 cggcttccgc gcgctcaagg tctggctcgt gctccgccag gccggccgcg agggctacgc
     1861 gcgcgcgatc gcgcaggacg tcgagctcgc gcgggagctt catcggctcg ccgaggccga
     1921 tccggagctc gaggcatgga cgacgaggct cagcatcacg acgttccgct acgtgccccg
     1981 cggggtcgag gccgggacgc ccgccggcga ggagctcgcg aaccgcgtga acgaggagct
     2041 cctcgggcgc ctccagtcgg gcggcgaggt cttcctctcg aacgcggtcg tgcgcggcgc
     2101 gtacctcctc cgcgcctgcg tggtgaactt ccggacgggc ctccgggacg tccgggccat
     2161 cccagagatc gtcaagcggg tcggcgccga ggtcgcggcc gagctccgct aggcccgacc
     2221 gacgcggcgc actgtgaccg aatcgtcacg cttccggcgc attccgcgcg cctcacgtgc
     2281 gtttgtcggc gctagcctga gggcggtagg cgggcgggtc gggcccgtac tagacgcgcg
     2341 agtaaccaca agccacggcc gccctcagaa gaccacgacc gagcgggcgc gctctctgcg
     2401 gcgcatggcc tcgaaggcct cttccacgcc ctccagctcg atccgatgcg agacgagccg
     2461 gtcgagcggc agcttcccgg ccaggtagag ctcggcgatg cggggaaagt cgagcgccgg
     2521 catcgcgccg ccgtagatcg agccgacaag cgtcttcccg gcgtccacga aggagtagac
     2581 gtcgaacgac gcgcgcaccc cggccgccgt gagcccgacg aggacgccga cgccgcccga
     2641 ccgaagcagc gacggcaccg cctcgaccgt ctcgaccagc ccgatcgcct cgaagacgta
     2701 gtcggccccg ccgggcgcga cgagcacgcg cgcctcgtcg agcgtcccga ccgcatgcga
     2761 cgccccggcc gacgacgcga gcgcgagctt ctcctccgac acgtcgaccg cgacgatcgg
     2821 atgcgccccc gcgagcgccg cccccatcac gaccgaaagc cccacgcccc cgcacccgac
     2881 gaccacgacc gacgcacccg gctcaacgcg cgccgtgttc cagaccgccc ccacccccgt
     2941 cgtgacggcg cagccgatga gcgccgcgac ctcccagggc acctcgcgcg gaaccgggat
     3001 cgccgccgcc tcggggacga ccgagcgctc cgccatcgag ccgaccgcga gataggtgaa
     3061 gacgtcctcg ccggagccgg gccgccggaa gcgcgtcgtc ccgtcgtcca tcacgcactg
     3121 gcccgcgcgg gtcccgctgc aggcccacgc ccgtccggcg aggcagtgcg cgcaccgccc
     3181 gcatgcgtag aaccacgaga ggacgacgtg gtcgccgacg gcgacgccgc gcacgccgtc
     3241 cccgaccgcc tcgacgacgc ccgcgccctc gtggcccatg acgaccggcg tcgggcgcgg
     3301 ccactcgccg tcgaccacgt ggagatccga gtggcagacc ccgctcgcgc cgagccggac
     3361 gagcacctcg cgcgcccgcg gcgcctcgag ctcgagctcc tcgacgctca cccgcccggg
     3421 cccctcgaac acggcggcgc gcactccgtc caccggcgga tcctacgacg ccgctactcc
     3481 ccgggcgggg cgcggtctac gattccctca tcgcaacgca tccggcgcac gcggacgagg
     3541 gtcgtgtcct cacggagatc atctcgacgg tcgggtcgtc gctcgacctg gacgaggtgc
     3601 tcgaggccgt cgtcgccctg ctctccgacg cgagcgccgt ccacgcgacg ttcgtctact
     3661 tcctcgagga cggcggcgag cggctcgtcc tcaaggcggc gtccgacccg tacgggcgct
     3721 tcgtcgaccg gatcgcgctc gagcggggcg aggggctcgc gtggtgggcg gccgagcggc
     3781 gcgagccggg gttcatcctc gacaacgcgc tcgacgaccc gcgcttcaag cacgtgccgg
     3841 agctcgagga ggagcgcttc cagtccctcc tctcggtccc gatcctcggc aaggacggcg
     3901 gcgtgatcgg cgtgatcacg ctccacacgg aggcaccgcg cgagttcacc gagccggaga
     3961 tcgacttcct cgtctcgagc gcgtcgctcg tcgccggcgc gatcgagaac gcgcgcctct
     4021 acgaggagat gcgccgccgc gtcgacgagc tcgagcacct gaccgagctc ggcgagacga
     4081 tcacgcgcgc cgagacggtc gaggagctcc tccccgcggt cgccgagcgg agcctctcgc
     4141 tcctgcgggc gcgctcctgc cacctctatc tgctcgacgc cggctccgag gagctgcacc
     4201 tgcgcgcgtc cgccccgccg ggcgcggagg cgcccgcgtc ggtcggcctc gccgggctcg
     4261 gccccgagct cgcgcggagc gggcgctccg cctccgtcgc cgtcccgctc gtcgcgaacg
     4321 acgagctgct cgggctgctg cgcgccgagg gcacctccgc ggtcgacctc gcccgcgcgg
     4381 ccgcgaacca gaccgccgtc gcgctgaaga agatcgagct gatcgagcgc ctgacggaga
     4441 agaaccggat ccgcgacttc ttcgactcgc tcgcgcgcgg gcggatcggg cccggcgtcg
     4501 aaggccgcgc cgggcggctc gggatcgacc tcacggcgcc gcatctcgtc ctcgtcgcct
     4561 cgccgccgga cgacgcgctc gagaaggcgc tcgcgcgcgc cgcccccggc tcgctcctcg
     4621 accgccgcga cgacgcgctc cgcgggctgc tccggatccc gcacgcgggc gaggagacgc
     4681 tcgtcgcggc cgttcgcgac gccgagcggg cgcccgagac cgccgtcggc ctctcgaacc
     4741 cgtgccgcgg ggcggagagc ttcgcggcgg gcttcgagga ggcgacccac gcgctcctcg
     4801 gcagctcagt cgtccgcggg gagcccgccg tcatgacgta cgaggagctc gggccgtaca
     4861 agtacctcct gcgcatgtcg ttcgagacgg ggatgcgcga ccgccaccgc gaggccgtct
     4921 cgcggctcgc ggactacgac cgccgccgct cgaccgcgct cctgaagacg ctcgaggagt
     4981 tcctccgccg gcgcggcaac atcagcgcga ccgcggaggc gctgtacatc cacccgaaca
     5041 ccctgcgcca gcggctgcga cggatcggag agctgaccgg gatcgacctc cgccgcgacg
     5101 actggctcat ggtcgagatc gcggtcaaga tcgcgaagct cgaagaggtg gcgggggaca
     5161 caatccgccg cgacctcatg tagcgagcgg ccatcgccgc ggcggcgggg ctcctttatc
     5221 ttcggatgca gccgaacccc gtccacgagg aggacacatg gccgttgcgg cagaaacgcc
     5281 gacgctcgat gacatccgca aggacgtggc cgacggcgtc gagttcttct ttgcgcaatt
     5341 cgtcgacatg cacgcgcgcc cgagcgcgaa gctcgtcccg gccggaaacc tcgacgatct
     5401 cctgagcgag ggcgccgggt tcgccgggtt cgccgccggc gacatcggcc aggtgcccgc
     5461 cgacccggac atcgcggcga tccccgacct ccgctcctac acgcgcgtcc cctgggagcc
     5521 cagtgtcgcg cgcttcgcgt gcgacatcca cgtcgagggc gaggagtggc cgtacgaccc
     5581 gcgcacgatc ctgcgccgtc agctcgagcg cgcgcgggag aagggcttcg agttccggat
     5641 ggggctcgag ctcgagtact tcctcgtcga gctgcacgag gacggctcga tcacggtcgc
     5701 ggacgagctc gacacgctcg agaagccctg ctacgacctg aacggcctct cgcgccacta
     5761 cgagttcctg accaccgtct cgcgctacgt gaacgcgctc ggctggggca actacgcgaa
     5821 cgaccacgag gacgcgaacg gccagttcga gcagaacttc acgtacgcgg acgcgctcac
     5881 gagctgcgac cgggcgatct tcttccgcta catggttcac cagctcgccg agaagtgggg
     5941 ccgccaggtc ggccggcgcc tgctcgcgac gttcatgccg aagccgttct cgcacctgac
     6001 gggcaacggg tgccacttcc acatgtcgct ctgggacggc gacacgaacc tcttcctcga
     6061 cgaggacgac cc
//
LOCUS       DASTFC010000339         5142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_768837,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000339 DASTFC010000000
VERSION     DASTFC010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768837"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFO88_10020"
     CDS             complement(<1..390)
                     /locus_tag="VFO88_10020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9545900.1"
                     /translation="MGPGLYNRSIVSVIIPAYNVGGLLSTSVKSALSQTETNIEVVIV
                     DDCSADNTREIAHEIAREDDRVRVLGNDENVGPGESRNRAIDAARGDWVAVLDADDAW
                     LPERLERLTPLLAEADVVTDDIVIEEAG"
     gene            397..1116
                     /locus_tag="VFO88_10025"
     CDS             397..1116
                     /locus_tag="VFO88_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9545901.1"
                     /translation="MWAARVTSRAKEVGPESQAPAVAAAGAKPLDAWWTVLVVDPVAL
                     RLLPALAARSSITPLRLTLLGAAISAASIPAFATEHFVLGAVLFQVGFFFDCLDGKLA
                     RMRGETSRLGAFLDQALDVLVRTGAFVALAWHAFPDSRIAPALVAAIVLLEAWLRTYP
                     TEAGPGSAGTALPGALGRARAAMARRRLVLLPATVDLEALSLFLAPLTGSIELIRIGV
                     VVAMAGYAFYGLLHVRRIVRG"
     gene            1116..1859
                     /locus_tag="VFO88_10030"
     CDS             1116..1859
                     /locus_tag="VFO88_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET9545902.1"
                     /translation="MPAAVVILAAGRGSRLAGDVPKWFLRPGGRTIAEVQLDGIRRGV
                     AHWTRILVVTGFRPELFDADAHGVELVHNQDWATRNNWYTLAVALEHLAREGWNGAVC
                     VLNSDLLIGPEVVARFLEAAEADPRSLLAVDPGAPATDEQMKVAVRDERVVDIGKGDL
                     SAPPAGEYVGLAKIGAGDVPTLAAILDGFAADPAHADEWYEAAFREAMHAGVEFGVFP
                     VDSGRWIEIDDDADLERAAAIAALLEDES"
     gene            1859..2896
                     /locus_tag="VFO88_10035"
     CDS             1859..2896
                     /locus_tag="VFO88_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9545903.1"
                     /translation="MRTDRSVSVPTLLEIAPGTVDELGALLAGTDLDLARPLVGTGGE
                     QTAEVAGQAREQLANAWVLPGLDGDPASVEAVVAEIGGRQATCCLAVGGGRVIDTVKY
                     ASAQTGVPFVSVPTALSHDGICSPIASLVGRDGRRQSLAAVMPAAVVVDLAVVRAGPP
                     RATRAGAGDLLSNLTALQDWRLAAARGRDTFDGYSALIAEASAHAFLYLAGAAEELSL
                     ETLARGLVLSGLAMATAGTSRPCSGAEHLISHSLDGLLRADARLHGEQVALGTLIAAQ
                     AHGGVPGELRAAYETLDLPRRCADLDLEPDVVVEAVMAAPATRPDRYTILDEVDLSRD
                     AVEELVARALD"
     gene            complement(2893..3798)
                     /locus_tag="VFO88_10040"
     CDS             complement(2893..3798)
                     /locus_tag="VFO88_10040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9545904.1"
                     /translation="MSDVDVVLVNYRSGPKLAAVVEEARRWLGGRAAVTIVDNSPGDG
                     SVDAVAGEVQVIANEENRGFAAAVNQAVRATSGPYVLLLNPDVAGIRGEFESIESVFA
                     ADADVAAVAPRLVGADGALQRNARREPRFGDLIVNALGLRGRFPESRRLRANQYGDWD
                     YDTERVVDAVTGAFLVLGRAALDAVGPFDERFFVYAEETDWLVRAKRLGWKTVLTPKV
                     EAKHEARGSTDADETALTLLLVESQHAYVGKHFGRRRALLFRGALLGIDSARWALAAA
                     RGPSRRAHRRALAERLRLHAGGTLR"
     gene            complement(3795..5036)
                     /locus_tag="VFO88_10045"
     CDS             complement(3795..5036)
                     /locus_tag="VFO88_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9545905.1"
                     /translation="MAADALRVLVLAPFPPRLDGRHGGSRVTAGFVTALAERHRVALF
                     HLGRGPLDVADDAVRAACEPLVRCELPERPGGGLGEVGYQVRTVGPLLVGRPRWATLA
                     WSRACAARLALLAREFRPDVIQCEFAVMTGYLPALADVPGARVLVAHEPAAARAAENA
                     TARLGGRRRARLAARLEARAWRRFERQALERVDAAVAFTETDRAALAALGATTPLHVI
                     PFGIDVPPEPLDPAGADERETLVFVANFVHGPNVEAARRLVLELLPRVRRERPGVVVE
                     LVGRAPPPAVRQLAGDGVTVTGEVPSVTPYLDRASVVVAPLAIGGGIRVKTIEALAYG
                     KPVVATPLAAAGLPVTDGEHLRLAETDDELARATLALLADPEGRAALGRRARAWALEH
                     AGWDATAAAYERLYRSLGAGS"
BASE COUNT          632 a         1933 c         1909 g          668 t
ORIGIN      
        1 cccggcctcc tcgatcacga tgtcgtcggt cacgacgtcc gcctccgcga ggaggggcgt
       61 cagccgctcg agccgctcgg gcagccaggc gtcgtccgcg tcgagcaccg cgacccagtc
      121 gccgcgcgcc gcgtcgatcg cccggttgcg gctctccccc gggccgacgt tctcgtcgtt
      181 cccgagcacg cgcacacgat cgtcctctcg cgcaatttcg tgtgctattt cgcgtgtgtt
      241 atccgccgag caatcgtcga cgattacgac ttcgatattc gtttccgtct ggctcagcgc
      301 cgacttcacc gacgtggaaa gaaggccgcc gacattgtac gccggtatga tgacgctgac
      361 gatggagcga ttgtacagac ccggtcccac gaccccgtgt gggccgcacg cgtgacgagc
      421 cgggcgaagg aggtcgggcc ggagtcgcag gcgccggccg tggccgcggc cggcgcaaag
      481 ccgctcgacg cctggtggac ggtcctcgtc gtcgaccctg tcgcgctgcg cctgctgccc
      541 gcgctcgcgg cgcgctcgtc catcacgccg cttcgcctca cgctcctcgg cgccgcgatc
      601 tccgccgcct ctatccccgc cttcgcgacc gagcatttcg tcctcggagc ggtgctcttc
      661 caggtcggat tcttcttcga ctgcctcgat gggaagctcg cacggatgcg cggcgagacc
      721 tcgcggctcg gggcgtttct cgaccaggcg ctcgacgtgc tcgttcgcac gggcgccttc
      781 gtcgcgctgg cctggcacgc gttccccgac tcgcggatcg cgcccgcgct cgtcgcggcg
      841 atcgtcctcc tcgaggcgtg gctccgcacg taccccaccg aggccggtcc cggatccgcc
      901 ggcacggcgc ttcccggcgc gctcgggcgg gcccgcgccg cgatggcccg acggcggctc
      961 gtcctgctcc cagccacggt cgacctggag gcgctctcgc tcttcctggc gccgctgacg
     1021 gggagcatcg agctgatccg aatcggcgtc gtcgtcgcga tggccggcta cgcgttctac
     1081 ggcctgctgc acgtccggcg aatcgtccgc gggtgatgcc cgccgccgtc gtgatcctcg
     1141 ccgcgggccg tgggagccgc cttgcgggcg acgtgcccaa gtggttcctc cggccgggcg
     1201 ggcggacgat cgccgaggtg cagctcgacg gcatccggcg cggcgtggcc cactggacgc
     1261 ggatcctcgt cgtcacgggg ttccggcccg agctcttcga cgcggacgcg cacggcgtcg
     1321 agctcgtcca caaccaggac tgggcgactc ggaacaactg gtacacgctc gccgtcgcgc
     1381 tcgagcacct cgcccgcgaa ggctggaacg gcgccgtttg cgtgctgaac tcggatctcc
     1441 tcatcgggcc ggaggtcgtc gcgcggttcc tggaggcggc cgaggccgat ccgcgcagcc
     1501 ttctcgcggt cgaccccggc gccccggcca cggacgagca gatgaaggtc gccgttcggg
     1561 acgagcgggt cgtcgacatc ggcaaggggg acctctcggc cccgccggcg ggcgagtacg
     1621 tcggcctcgc gaagatcggc gcgggcgacg tcccgaccct cgccgccatc ctcgacgggt
     1681 tcgcggccga tcccgctcac gcggacgagt ggtacgaggc cgcgttccgc gaggcgatgc
     1741 acgcgggggt cgagttcggc gtcttccctg tcgattccgg acggtggatc gagatcgacg
     1801 acgacgcaga cctcgagcgg gccgcggcga tcgcggcgct cctggaggac gagagctgat
     1861 gcggacggac agatcggtga gcgttccgac gctcctcgag atcgcgccgg gcacggtgga
     1921 cgagctcggc gcgctcctcg ccggcacgga cctcgacctc gcgcgcccgc tcgtcgggac
     1981 gggcggcgag cagaccgcgg aggtggccgg gcaggcgcgg gagcagctcg cgaacgcgtg
     2041 ggttctcccc ggcctcgacg gcgacccggc gagcgtcgag gcggtcgtgg cggagatcgg
     2101 cggccgccag gcgacgtgct gcctcgccgt cggcggcggc cgggtgatcg acaccgtgaa
     2161 atatgcgagc gcgcagacgg gcgtgccgtt cgtgagcgtc ccgacagcgc tctcgcacga
     2221 cgggatctgc tcgccgatcg cctcgctcgt cggccgggac ggccggcgcc agagcctcgc
     2281 ggccgtcatg ccggccgcgg tcgtcgtcga cctcgcggtc gtgcgggcgg gcccgccgcg
     2341 ggcgacgcgg gccggcgcgg gcgacctcct gagcaacctg acggcgctcc aggactggcg
     2401 gctcgcggcc gcgcgcgggc gcgacacgtt cgacgggtac tcggcgctca tcgcggaggc
     2461 ctccgcgcac gccttcctct acctcgccgg ggcggccgag gagctctcgc tcgagacgct
     2521 cgcgcgcggg ctcgtgctca gcggcctcgc gatggcgacg gccgggacga gtcggccgtg
     2581 ctcgggcgcc gagcatctca tcagccactc gctcgacggg ctcctgcgcg cggacgcccg
     2641 gctgcacggg gagcaggtcg cgctcgggac gctgatcgcc gcccaggcgc acggcggcgt
     2701 gcccggggag ctgcgcgccg cctacgagac gctcgacctc cccaggcgct gcgccgacct
     2761 cgacctcgag ccggacgtcg tcgtggaggc ggtgatggcg gcgcccgcga cgcggccaga
     2821 ccgctacacg atcctcgacg aggtcgatct ctcgcgtgac gccgtggagg agctcgtggc
     2881 gcgcgcgctc gattagcgaa gcgtgccgcc cgcgtgcagc cggagccgct ccgcgagcgc
     2941 ccggcggtgc gcccgcctgg acgggccccg cgcggccgca agcgcccagc gcgccgagtc
     3001 gatcccgagg agcgcgccgc ggaagaggag cgcgcgccgc cgcccgaagt gcttcccgac
     3061 gtacgcgtgc tggctctcga cgaggaggag cgtcagggcg gtctcgtccg cgtcggtgct
     3121 tccgcgcgcc tcgtgcttcg cctcgacctt cggcgtgagg acggtcttcc agccgaggcg
     3181 cttcgcgcgg acgagccagt cggtctcctc ggcatagacg aagaagcgct cgtcgaaggg
     3241 gccgaccgcg tcgagggccg cgcggcccag gacgagaaag gcgcccgtga cggcgtcgac
     3301 cacgcgttcc gtgtcgtagt cccagtcgcc gtactgattc gcgcggaggc gccgtgactc
     3361 cggaaagcgt ccgcgcaggc cgagggcgtt cacgatgagg tcgccgaagc gcggctcgcg
     3421 acgcgcattg cgctggagcg cgccgtcggc gccgacgagc cgcggtgcca cggccgcgac
     3481 gtcggcgtcg gccgcgaaca ccgactcgat cgactcgaac tcgccgcgga tccccgccac
     3541 gtcagggttc aggagcagga cgtacggccc cgaggtcgcg cggacggcct ggttcacggc
     3601 agcggcgaag ccgcggttct cctcgttcgc gatcacctgg acttcccccg cgacggcgtc
     3661 gaccgagccg tcgccgggcg agttgtccac gatcgtgacc gccgcgcgcc cgccgagcca
     3721 gcgccgcgcc tcctcgacga cggcggcgag cttcgggccg ctcctgtagt tcacgaggac
     3781 gacgtcgacg tcgctcacga accggctccg agcgagcggt agagccgctc gtaggcggcg
     3841 gcggtcgcgt cccagcccgc gtgctcgagc gcccacgcgc gggcgcggcg gcccagcgcc
     3901 gcgcgccctt cgggatcggc gagcaacgcg agcgtcgcgc gcgcgagctc gtcgtccgtc
     3961 tccgcgagcc ggaggtgctc gccgtccgtc accgggagcc ccgccgcggc gagcggcgtc
     4021 gcgacgaccg gcttgccgta cgcgagcgcc tcgatcgtct tgacccggat cccgccgccg
     4081 atcgcgagcg gagcgacgac gacgctcgcg cggtcgaggt acggcgtgac cgacggcacc
     4141 tccccggtca cggtcacgcc gtcccccgcg agctgccgga cggcgggagg aggcgcgcgc
     4201 ccgacgagct cgacgacgac gccgggccgc tcgcggcgga cgcgcggaag cagctcgagc
     4261 acgagccgcc gagccgcctc gacgttcggg ccgtgcacga agttcgcgac gaagacgagc
     4321 gtctcgcgct cgtccgcgcc ggccgggtcg agcggctcgg gcggcacgtc gatcccgaac
     4381 gggatcacgt gcagcggcgt cgtcgcgccg agcgcggcga gcgccgcccg gtcggtctcg
     4441 gtgaacgcga cggcggcgtc gacgcgctcg agcgcctgcc gctcgaaccg ccgccaggcc
     4501 cgcgcctcca ggcgggccgc gagccgggca cgccggcgcc cgcccagccg cgcggtcgcg
     4561 ttctcggcgg cgcgcgccgc cgccggctcg tgcgcgacga gcacgcgggc gcccggcacg
     4621 tccgcgagcg ccggcaggta gcccgtcatg accgcgaact cgcactggat cacgtcgggg
     4681 cgaaactcgc gcgcgaggag cgcgagacgg gcggcgcagg cacggctcca cgcgagcgtg
     4741 gcccagcggg ggcgaccgac gaggagcggc ccgaccgtcc ggacctggta gccgacctcg
     4801 cccaggccgc cgcccggccg ctcgggcagc tcgcagcgaa cgagcggctc gcacgccgcc
     4861 cgcaccgcgt cgtcggcgac gtccagcggc ccgcgcccga ggtggaagag cgcgacccgg
     4921 tgccgctccg cgagcgcggt cacgaagccc gcggtgaccc ggcttcctcc gtggcgaccg
     4981 tcgagccgcg gcgggaaggg tgcgaggacg aggacgcgga gcgcgtcggc tgccatggtc
     5041 ggattatccg gcggcgcgga gcgcgcgggc gattctccag gcgaagcggc cgagcccgtc
     5101 cccggcgcgc ggctcgacac ggccgatcag gagctcgtcg tc
//
LOCUS       DASTFC010000340         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_637226,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000340 DASTFC010000000
VERSION     DASTFC010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637226"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="VFO88_10050"
     CDS             complement(<1..1833)
                     /locus_tag="VFO88_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545906.1"
                     /translation="MSTPAALEGARETLPPRPAPHPGPDEAKRPRRRPAPSLALVVLF
                     AVAALYHVLNGIFHATPAVFTDELLHSELARSLAAGDGLAVRDEGHFFPSFLPALAQA
                     PAWLFGSAETSYAVAKGLNALVMSAAVFPAYWLARALVRPSYALLVAAAAVAAPAMLY
                     HAYLLSEALAYPVFLLALAVAVRELATPSRAWGIALVGVFALAAATRIQFAALPLVWA
                     LVVALGGRRELRRHLLPLAGLGLLGAAGLAAGPSVLGIYAGVTELLADPAGIVRWASL
                     TAILLPFAAGVVVVPGAALGLAAALARPRGAAEGAFARFALGVGLAFVALAGLVAAGD
                     SERPLERYAIYLAPLALVLFFAYVERGAPWRRAYAGLALALGLAAWLVPFPGLADFRF
                     SFDSPMLSAYGTLAHAIGTANAATVFAAVPLALLAAAAFVPLTRRAAHVLGTVAIALL
                     LAAGAVAYAGDRAMTDRALAAWAPAEPDWLDRSGHGRADVLVLPGSPAYFLWTLESWN
                     RDTGRPLWLDAEPPDHDPYPSGRASVAEDGTLLVDGAPAPAGLLVLADFGSRIALEGD
                     VLAEPRDGLTLVRVPESPTVGALARGLFPDGWAKGDLRYRVWPEA"
     gene            1872..>2720
                     /locus_tag="VFO88_10055"
     CDS             1872..>2720
                     /locus_tag="VFO88_10055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9545907.1"
                     /translation="MVPAFSVSVVVVAHRAGAALGRCLDSLEQQDGELEILVVDNGAE
                     GDEIEEAAGRRRVRIVGTGENLGFAGGCNAGAWASRGEVLVFLNPDTVAGDGAIDALA
                     VTLEDPTIGVAMARLRLMDSPTLLNSRGNVLHVAGFAWVGGHGEPVETAPDLVDVPSA
                     SGAALAVRADLFRELGGFTEELFLYGEDVELAWRARLRGLRVVMNPRADVFHEYDFAR
                     NDSKRYFLERNRLVFVLSAFSWRTLALLWPVLVAAEMGTSALAVRDRWLRQKAAAWGW
                     LARHVRW"
BASE COUNT          369 a          947 c         1122 g          282 t
ORIGIN      
        1 cgcctccggc cagacgcggt agcgcagatc ccccttcgcc cagccgtccg ggaagaggcc
       61 gcgggccagc gcgccgaccg tcgggctctc ggggacgcgc acgagcgtga gcccgtcgcg
      121 gggctcggcg aggacgtctc cttcgagcgc gatccgcgac ccgaagtcgg cgaggacgag
      181 caggccggcc ggggcggggg ctccgtcgac gaggagcgtc ccgtcctcgg ccacgctcgc
      241 gcggccggag gggtacgggt cgtggtcggg cggctccgcg tcgagccaga gcggccggcc
      301 ggtgtcgcgg ttccaggact cgagcgtcca gaggaagtac gcggggctgc cgggcaggac
      361 gaggacgtcc gcgcggccgt ggccggagcg gtcgagccag tcgggctcgg ccggggccca
      421 ggccgccagc gcgcggtcgg tcatcgcccg gtctcccgcg tacgcgacgg cgcccgcggc
      481 gaggagcagg gcgatcgcca ccgtcccgag cacgtgcgcg gcgcggcgcg tgagcgggac
      541 gaacgccgcg gccgcgagga gggcgagcgg tacggcggcg aagaccgtcg cggcgttcgc
      601 ggtcccgatc gcgtgggcga gcgtcccgta ggcggacagc atcggcgagt cgaaggagaa
      661 gcgaaagtcg gcgaggcccg ggaacgggac gagccacgcg gcgagcccga gcgcgagcgc
      721 gagacccgcg tacgcgcggc gccacggcgc tccccgttcg acgtacgcga agaagaggac
      781 gagcgcgagc ggcgcgaggt agatcgcgta gcgctcgagc ggccgttccg agtcgccggc
      841 cgcgacgagc ccggcgagcg cgacgaacgc gagtcccacg ccgagcgcga accgcgcgaa
      901 cgcgccctcc gcggcgccgc gcggacgggc gagcgccgcg gcgagcccga gcgcggctcc
      961 cgggacgacg acgacccccg ccgcgaacgg aaggaggatc gcggtgagcg aggcccaccg
     1021 gacgatcccg gccgggtcgg ccaggagctc cgtgacgccc gcgtagatgc cgaggacgga
     1081 cggcccggcc gcgaggccgg ccgcgccgag gagcccgagc ccggcgagcg ggaggaggtg
     1141 gcgccgcagc tcccggcgcc cgccgagggc cacgacgagc gcccacacga gcgggagcgc
     1201 cgcgaactgg atccgcgtcg ccgcggcgag cgcgaacacg ccgacgagcg cgatccccca
     1261 ggcgcgcgac ggcgtcgcga gctcccgaac ggcgaccgcg agcgcgagga ggaagaccgg
     1321 gtacgcgagc gcctccgaga ggaggtacgc gtggtagagc atcgccggtg ccgcgaccgc
     1381 cgccgccgcg acgaggagcg cgtaggacgg acggacgagc gctcgcgcca gccagtaggc
     1441 ggggaagacg gcggcgctca tcacgagcgc gttcagcccc ttcgcgaccg cgtacgaggt
     1501 ctccgcgctg ccgaagagcc acgccggcgc ctgcgcgagc gcggggagga acgaggggaa
     1561 gaagtgcccc tcgtcgcgca ccgcgaggcc gtcgccggcc gcgagggagc gggccagctc
     1621 cgaatggagg agctcgtccg tgaagaccgc gggcgtcgcg tggaagatcc cgttgagcac
     1681 gtggtagagc gccgcgacgg cgaagaggac gacgagcgcg agcgaggggg cggggcggcg
     1741 gcgcggccgc ttcgcctcgt ccgggccggg gtggggggca ggccgcggcg ggagcgtctc
     1801 gcgcgcgccc tcgagggccg cgggcgtgct catgagcggt ctatcggcag ccgatacatc
     1861 gctctgtagc cgtggttccc gcgttctccg tctccgtcgt cgtcgtcgcc caccgggcgg
     1921 gggcggcgct cgggcgctgc ctcgactcgc tcgagcagca ggacggggag ctcgagatcc
     1981 tcgtggtcga caacggcgcg gaaggcgacg agatcgagga ggcggctggg cgacggcgcg
     2041 tgcggatcgt cggcacgggc gagaacctcg gcttcgccgg cggctgcaac gcgggggcgt
     2101 gggcgtcgcg cggcgaggtg ctcgtgttcc tgaacccgga cacggtcgcc ggcgacggcg
     2161 cgatcgacgc gctcgcggtg acgctcgagg acccgacgat cggcgtcgcg atggcccggc
     2221 tgcgtctcat ggattcgccg acgctcctga actcgcgcgg caacgtcctc cacgtggccg
     2281 gcttcgcctg ggtcggcggg cacggggagc cggtcgagac ggcgcccgac ctcgtcgacg
     2341 tgccgtccgc gagcggcgcg gcgctggcgg tgcgcgcgga tctcttccgc gagctgggcg
     2401 gcttcaccga ggagctcttc ctctacggcg aggacgtcga gctcgcgtgg cgggcgcggc
     2461 tgcgcggcct gcgcgtcgtc atgaacccgc gcgcggacgt gttccacgag tacgacttcg
     2521 cgcgcaacga ctcgaagcgg tacttcctcg agcggaaccg gctcgtgttc gtcctgtcgg
     2581 cgttctcgtg gcggacgctc gcgctcctct ggcccgtgct cgtcgcggcg gagatgggga
     2641 cgtcggcgct cgccgtgcgc gaccggtggc tgcgccagaa ggcggcggcg tgggggtggc
     2701 tcgcgcggca cgtccgctgg
//
LOCUS       DASTFC010000341         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_999754,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000341 DASTFC010000000
VERSION     DASTFC010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999754"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VFO88_10060"
     CDS             <1..1244
                     /locus_tag="VFO88_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9545908.1"
                     /translation="ASVAATLRLMTRTTPSLRGRHRVVVAGGGVAGLETVLALSRLAP
                     GLVDVELIAPEHHFWYRPLAVAEPFDAGRVVRFELAALAAEVGATYTPGELVGVRTDE
                     RVGELAQGADVPYDSLVLAAGAVPEEAVHGALTFRGPADTERFRGLLDEIEEAGSGTL
                     AFVHPGGTGWPLPLYELALMTAAELAERAPDVRVRLVTREAAPLELFGGAASDAVRDL
                     LVQRRIEISTGLYASSWANGALETAPDGPVEATWAVALPTLRGPDLAGVPRGRGGFVP
                     TDRHGRVHGLEGVYAAGDLTAFPIKQGGLAAQQADAVAESIAAEAGARIDPRPFEPVL
                     RGLLLTGGVPAYLRAELGGGRGETSLVANEALWWPPGKIVGRELAPFLARVAGVEPLA
                     PLEAPGVVAIEIPLETDPVAA"
     gene            1360..2226
                     /locus_tag="VFO88_10065"
     CDS             1360..2226
                     /locus_tag="VFO88_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545909.1"
                     /translation="MRSRLEDYFIRNAAGIVDGLAEERGGVLADSLGELEERSMSVAA
                     AEAKAAELDRRLARFGPADADEPRQIEHLLAELEAREAELLSLRPRVQELETEVSASR
                     EARGRAEAALADQVKRFQAQEKWVDDLRQRVERADAARRRAEEALKTAQDGERQRART
                     ALELGQERKALAQRDAELARREARLARLEALEGRDVELQQVADRLAARETALAEREAA
                     LARREEEAAATRLPSVEPESVRRGEERLAELRERFARREEGRTAESPAEPTSLFGPED
                     RRLSAFDPDSAA"
     gene            2258..>2560
                     /locus_tag="VFO88_10070"
     CDS             2258..>2560
                     /locus_tag="VFO88_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9545910.1"
                     /translation="MIVARTEHPDWLSNAYLVADEPGGRGVLVDGNGLLDELVLRAEA
                     EGIELTHVLLTHGHHDHVEGVREAAERLGVPVVAAAGIGGADEEAGDGWTLRTGGLE"
BASE COUNT          305 a          948 c         1010 g          297 t
ORIGIN      
        1 gcgcatccgt cgccgcgaca ctgcggctca tgacgaggac gacgccatcc ctccgcggcc
       61 ggcaccgtgt cgtggtcgcc ggcggcggcg tcgcagggct cgagaccgtg ctcgcgctga
      121 gccggctcgc gcccggcctc gtcgacgtcg agctcatcgc gccggagcac cacttctggt
      181 accgaccgct cgccgtggcg gagccgttcg acgcgggacg cgtcgtccgg ttcgagctcg
      241 cggcgctcgc ggccgaggtc ggcgcgacgt acacgcccgg ggagctcgtc ggcgtgcgta
      301 cggacgagcg ggtcggggag ctggctcaag gggccgacgt cccctacgac tcgctcgtcc
      361 tggccgccgg cgcggtgccg gaggaggccg tccacggagc gctcacgttc cggggccccg
      421 ccgacaccga gcgcttccgc ggcctgctcg acgagatcga ggaggcgggc agcggaacgc
      481 tcgcgttcgt ccatccgggc gggaccggct ggccgctgcc gctgtacgag ctcgcgctca
      541 tgacggcggc cgagctggcc gagcgcgcgc cggacgtccg cgtccggctc gtcacccgcg
      601 aagcggctcc cctcgagctg ttcggcgggg ccgcgagcga cgccgtgcgc gacctcctcg
      661 tgcaacgccg gatcgagatc tcgacgggcc tctacgcctc ctcctgggcg aacggggccc
      721 tcgagaccgc gccggacggc ccggtcgagg ctacgtgggc agtcgccctc ccgaccctgc
      781 gtggccccga cctggccggc gtcccgcgag gacgcggggg gttcgtcccg accgaccggc
      841 acgggcgggt gcacgggctc gaaggggtct acgccgcggg cgacctgacc gcgttcccga
      901 tcaagcaggg cggcctcgcc gcccagcagg cggacgcggt cgcggagtcg atcgcggcgg
      961 aggccggagc gcggatcgat ccgcgcccgt tcgagccggt gctgcggggg ctcctcctca
     1021 cgggaggggt ccctgcgtac ctgcgggccg agctcggcgg cggccgcggc gagacgtcgc
     1081 tcgtcgcgaa cgaggcgctc tggtggcccc ccgggaagat cgtcggccgc gagctcgcgc
     1141 cgttcctcgc ccgggtcgca ggcgtcgagc cgctcgcgcc gctcgaggcg ccgggcgtcg
     1201 tcgcgatcga gatcccgctc gagaccgatc cggtcgcggc ctgagccggc cggcgccgag
     1261 tccctcgaac tggtgatgcg aggcgcgccc gcgcgccctt cacttgaaga gaccacgaac
     1321 ccgatctgcg gcgaccgggg gtgacccgcc ttggcgcgct tgcgcagcag actcgaggac
     1381 tacttcatca ggaacgccgc cgggatcgtc gacggcttgg cggaggagcg tggcggcgtg
     1441 ctcgccgact cgctcggcga gctcgaagag cggtcgatga gcgtggccgc cgcggaggcg
     1501 aaggcggccg agctcgaccg gcggctcgcc cgcttcggcc cggcggacgc ggacgagccg
     1561 aggcagatcg agcacctgct cgccgagctc gaggcccgtg aggccgagct cctctcgctg
     1621 cggccgcgcg tgcaggagct cgagaccgag gtctccgcgt cgcgcgaggc gcgcgggcgc
     1681 gctgaggcgg cgctcgccga ccaggtgaag cgcttccagg cgcaggagaa gtgggtcgac
     1741 gacctccggc agcgggtcga gcgcgcggac gccgcgcgcc ggcgcgccga ggaggcgctc
     1801 aagaccgccc aggacggcga gcggcagcgg gcccggacgg cgctcgagct cgggcaggag
     1861 cgcaaggccc tcgcccagcg cgacgccgag ctcgcccgcc gggaggcccg gctcgcgcgg
     1921 ctcgaggcgc tcgagggccg cgacgtcgag ctccagcagg tcgccgaccg gctggccgcg
     1981 cgggagacgg cgctcgccga gcgcgaggcc gctctcgctc ggcgcgagga ggaggccgcg
     2041 gcgacgcgcc ttccgtccgt cgagcccgag tccgtgcggc gcggcgagga gcgcctggcc
     2101 gagctgcgcg agcgtttcgc ccgccgggag gaggggcgca cggccgagtc gccggccgag
     2161 ccgacgtcgc tcttcgggcc cgaggaccgg cgcctgagcg cgttcgatcc cgactccgcc
     2221 gcgtagcctc gccgccgccg gtacggttcg gcgtggcgtg atcgtcgcgc ggaccgagca
     2281 tcccgactgg ctctcgaacg cctacctcgt cgccgacgag cccggcgggc ggggggtcct
     2341 cgtggacggg aacggcctcc tcgacgagct cgtcctccgc gccgaggccg aggggatcga
     2401 gctgacgcac gtcctcctga cgcacggcca ccacgaccac gtcgaaggcg tccgggaggc
     2461 ggcggagcgg ctcggcgtgc cggtcgtcgc cgccgcggga atcggcggtg cggacgagga
     2521 ggcgggcgac ggctggacgc tccgtaccgg cgggctcgag
//
LOCUS       DASTFC010000342        11262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_686272,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000342 DASTFC010000000
VERSION     DASTFC010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686272"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VFO88_10075"
     CDS             complement(<1..1254)
                     /locus_tag="VFO88_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9545911.1"
                     /translation="MIWPAAGALLAALVVAGAVAGPTDARRPNIVLVVTDDQRADATD
                     RMPRLKFRLAARGVRFANAFVTDPLCCPARASLLTGRSPRGTGVWSNRAPFGAWDAFR
                     SSGAERRTIAVALRRSGYRTALVGKYLNAYRGTTIPPGWSVWNAFSSGWTYYGYTLNL
                     DGRLVRYGGSPGDYSTDVLAARAVDFVRTSRAPFFLYFAPAAAHWPATPAPRHAGRRL
                     RLPPFAPPSLRRQSLRGKPRYVRRGARALLPRGKRFRGRQYRTLLAVDEAVDRILEAI
                     ERRGQLRNTLVLFTSDNGVMWGEHRMLAARKAVPYESSIRVPLLVRFDRAVARARTER
                     ALVSNLDVAATIAAAAGVGHAGEGRSLLPFLRDREPRGGRRERVLVESVGGESRSDPI
                     PAYCAVRSKAELYVLYGTNEEELYDL"
     gene            1338..1697
                     /locus_tag="VFO88_10080"
     CDS             1338..1697
                     /locus_tag="VFO88_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9545912.1"
                     /translation="MAKKNGRLGERIRAARAERGWKQKHLAAEVSVEPITVSRWERGA
                     TTPDLEVLRLVAGATGKPLSFFVDEGAPSAQASPGLADAATRIEAAAGRIAEESERLA
                     VLLSELVEELRAARSGS"
     gene            complement(1671..2516)
                     /locus_tag="VFO88_10085"
     CDS             complement(1671..2516)
                     /locus_tag="VFO88_10085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9545913.1"
                     /translation="MPPPREGAAFPEPGERLGPYTLAEPLGRGQVGAVFRAQGPPGEV
                     ALKVLHAGLAEDPSYRRRFERESEVARALDHPHLLRVVDHGEADGRPYLAAPFVAGGS
                     LADRLAAGPLEREELVRVVRHVASGLDALHRAGLLHRDVKPSNVLLREDGAAALGDFG
                     LARAKAHTVLTQEGRVAGTADYLAPEAIRGEPVGPRADVYALGCLAYECATGAPPFAA
                     ARSVVAACRAHLLEEPADPAAARADLPAPLAAALLTALAKEPERRPATGTAYAFLLRA
                     GARGA"
     gene            2581..4128
                     /locus_tag="VFO88_10090"
     CDS             2581..4128
                     /locus_tag="VFO88_10090"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9545914.1"
                     /translation="MWYHRPISSRARIGTEFAGYRIEALLGRGGTSTVYRAESPRLGV
                     RVALKILNPELSDDEIFRERFVREARVVARIEHPNVITIYDADAFGDELYIAMRYVPG
                     GDVTGALADGPLASERAVALLAQAASGLDTAHAHGLVHRDVKPANLMLDGGGRPGAPE
                     TVYVTDFGLTKHSQSGSSPTPTGVLLGTIDYVAPEQIEGRSPDGRADLYSLACVAVEC
                     LSGRRPFERDNEAAVLWAHMQEPPPSLSERAPELPKGVDEPIARALAKNPDERQSSCA
                     EFVEELREALAPFGRRPAKESAARPAPSRVRLLAAALAGVAAGAAGVGAAVVLGNGDE
                     AFPGGAGRTETVVSTVVTTVTGPEPELPSFIPQQFRNRCRSASPPTADFDSSYVCAPG
                     GAASTVRFSHAISGPLLASYLRRRMERVGLRAPASDRPIVAEGSCAEQVLPAIEHWFA
                     LGLAGHQSAGELPGASADGRVLCYEEGGVAHIEWTTGVLGVYAHASGPRFPRLYQWWR
                     TNSGPVS"
     gene            complement(4321..4402)
                     /locus_tag="VFO88_10095"
     tRNA            complement(4321..4402)
                     /locus_tag="VFO88_10095"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4366..4368),aa:Tyr,seq:gta)
     gene            4483..5667
                     /locus_tag="VFO88_10100"
     CDS             4483..5667
                     /locus_tag="VFO88_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET9545915.1"
                     /translation="MDLNALLAETVARGGSDLHLKVSSPPMARLDGSLGPIAETPLTD
                     ADLERALEIVAARTPQKLQLFQESGDLDTAYTAAGVGRFRVNGFRQRGSISFAFRHVP
                     DEVPTFEKLGLPRGVELLAGEHRGLVLVTGATGSGKSTTLAAMIDKINRTRKQHIVTI
                     EDPIEIIHRDQSSIVNQREVGLDTESFKQALRRVLRQDPDVILIGELRDEETAQTALQ
                     AAESGHFVLSTMHTVDAAETVGRLIEFFPPTKQLMLRQIIAGVLRGVVSQRLLPAKDG
                     GRLAAVEVMVNTARIADLIRDPLKTDGILEAIEEGGYHHMQSFSQHLVQLVLDERVEF
                     EVAATAATNRHDFEIAVQQAARLKNAESAAAAQTPPAAEPAANGAPHVEDELGTLRLA
                     GP"
     gene            5673..7115
                     /locus_tag="VFO88_10105"
     CDS             5673..7115
                     /locus_tag="VFO88_10105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HET9545916.1"
                     /translation="MLRRFTLLLSAALVAAFAVSGAQAAVPSPLPSYTTPNAPGAIVV
                     PFSVSTPPARPAQRSYAQLLGLWQEAGAAYGVPWEVLGAINKIESNFGRNMGPSSAGA
                     VGWMQFMPDTWTRWGMDGDGDGVANPWVPEDAVYAAARYLAAAGAHDDLYRAIFAYNH
                     ADWYVQDVLELAAVFAGGGGFDPTLGTGALGFTGGGFAGGADAVFRISDLETRLADAR
                     RAVKRLQERVSEVEDLLIELDTAVFEAEQRAGDPSLSDEEFQAVETEVTGLVLRQEAE
                     LERLAVRRARLEEAAQLVDDLRNAAQEQASTYSFSRTSAVDALGAPHFSGQYVFPVGG
                     GAAVVSVGADHHDYPAADIAAPEGSPLYALADGVVVDVYPTPTGRCGIGFSIQIVDGR
                     TFLYCHLSYLDPTVSQGTALAAGTPVGLVGSTGNSTGPHLHLQLVPSSGGYPQHEGWF
                     QSFAGVAFRWQDEAPPRAPGAPDGAIPFSR"
     gene            7173..8066
                     /locus_tag="VFO88_10110"
     CDS             7173..8066
                     /locus_tag="VFO88_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9545917.1"
                     /translation="MAAHLSALLPRLVALTLIWLLATSTITFAAGGRTTTPPPPPAAP
                     APKEPEYLVVPDVRRQAYVFAKGILADGGFGWRVTGPVKGYAANTVVSQKPAPGAKVE
                     DTGAPEIVLVLERNPAYPEHGLPENASPTPGTPVVLAGARDLAPAPQPEPPVEEQPAE
                     EPAPAQPEKPEKPEEPKDEQAPKKQKQEPADKKRTPAFQVDGAPAEPLDEIPLPERAR
                     RLEQRLAGKAKPTPALVNHWLYQHAWVVTGARFGWSGGAEALRILVRTDRDLQARWGI
                     GGKSLAVARAALAEVERRSSR"
     gene            8066..8599
                     /locus_tag="VFO88_10115"
     CDS             8066..8599
                     /locus_tag="VFO88_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9545918.1"
                     /translation="MRAGRVLRALRAAEGYTMIELLVVLMILTTVLTALTALFLAGAN
                     SELESNRRFQAQQDARLAADRLRREVHCAGGATVSTPSSVTVHLLGHCPTAIGNAPTN
                     VVYDVVADGGHYELRRAGVRIADHLVSSEVFTYTAPSAASRGLLGVDLQVNVRPNEGW
                     KTWHLQTDIVLRNTLRQ"
     gene            8596..10164
                     /locus_tag="VFO88_10120"
     CDS             8596..10164
                     /locus_tag="VFO88_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HET9545919.1"
                     /translation="MRIRLSNCGKLLRRQDGISLIMAIGILGVLSLAGTSTIYYASAN
                     ARSAEYSQENASAYDLAEAGINEILAVLGKPENNALEADLLPETTRTYDAGTVTWSGT
                     LDLATATWAITSTGRIKNPTGAVGDVTRTLTAKVPVTPTYTQPLNNPVWNYIFATRTG
                     TGGCDQTMNNNVGGGTRLYVAGNLCLSNNAAIETSPLIVHGKLWLDEGQTRVGSLQNR
                     VEAYVGQGCRKGQNLPAVIVPCPGDSQDVYSILAETGMPGVNSTPPIMAAPVVDWLNW
                     YQDAMPGPVTPCTSTNGAASGAVPTFDNNAMRDNSVGTVYNLTPATSYTCRVGPARTP
                     SGCYPPPAPSGTTQCTDYPVGEISWNATTRMLTVYGTIYLDGSAYVGNGLLNQYNGVA
                     TIYLSGTLYMNGKLCGGVSGSSCDFANWDPNKELITFVAGETSPGGTNKPDVGYSVEL
                     ENNAQLQGGLYASAAILLGNNAKTDGPMVASYVTMSNNVVGSDFPIITTVPVGMPGNP
                     AVYAQPNAPQLYSG"
     gene            complement(10226..>11262)
                     /locus_tag="VFO88_10125"
     CDS             complement(10226..>11262)
                     /locus_tag="VFO88_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET9545920.1"
                     /translation="IPKRLAVAVTTRLKVLAKLDIAERRKPQDGRISLNAKAAGRLLD
                     IRVAVLPSVEGEMVVMRLIDKSKRLPSLADLGFSDEMRTRLEEVIRKPTGALLVTGPT
                     CSGKSTTLYASLEQLNRPEINVITVEDPVEYRMLGLNQVQVNPKAGLTFASALRSILR
                     SDPDVVMVGEIRDVETAKMAIEAALTGHFVLSTLHTNDAPSALTRLNEMGVEPFLTGS
                     AVTAVVAQRLARKLCTHCCEMYIPTHEEMLNARFTPEQVAAADGVSLYRRKGCPRCNQ
                     TGYKGRIGIYQLMVMTEDLSRLAATHASRDEIERSAMEGGMKTLWDDGLAKVASGLTS
                     IEELGRVLVQ"
BASE COUNT         1609 a         4143 c         4058 g         1452 t
ORIGIN      
        1 gaggtcgtag agctcctcct cgttcgtccc gtagaggacg tagagctccg ccttgctccg
       61 caccgcgcag taggccggga tcgggtcgct tcggctctcg ccgcccacgc tctcgacgag
      121 cacccgctcg cgccggccgc cacgcggctc gcggtcgcgg aggaacggca ggaggctccg
      181 tccttcgccg gcgtgcccga cgccggccgc agccgcgatc gtcgccgcga cgtcgaggtt
      241 cgagacgagc gcgcgctcgg tgcgcgcccg cgcgacggcg cggtcgaagc gcacgaggag
      301 cgggacgcgg atcgagctct cgtacggcac tgccttgcgg gccgcgagca tccggtgctc
      361 gccccacatc acgccgttgt ccgacgtgaa gaggacgagc gtgttgcgga gctgcccgcg
      421 ccgctcgatc gcctcgagga tccggtcgac cgcctcgtcg accgcgagga gcgtccggta
      481 ctggcggccg cggaagcgct tcccgcgcgg gagcagcgcc cgggcgcccc ggcggacgta
      541 gcgcggcttg cccctcaggc tctgccggcg gaggctcggc ggcgcgaagg gcggcagccg
      601 aagccgccgg cccgcgtggc gcggagccgg cgtcgccggc cagtgcgcgg cggccggggc
      661 gaagtagagg aagaacggag cccggctcgt gcgcacgaag tcgaccgcgc gggcggcgag
      721 gacgtccgtc gagtaatcgc ccggcgagcc gccgtacctg acgaggcgcc cgtcgaggtt
      781 cagggtgtag ccgtagtacg tccagccgct cgagaacgcg ttccagacgc tccagccggg
      841 cgggatcgtc gtcccgcggt acgcgttcag gtacttgccg acgagcgcgg tgcggtagcc
      901 ggaccgcctg agcgcgaccg cgatcgtgcg ccgctcggcg ccggagctcc ggaacgcgtc
      961 ccaggcgccg aacggcgcgc ggttgctcca gacgccggtt ccgcgagggg agcgcccggt
     1021 gaggagcgag gcgcgcgccg ggcagcagag cgggtcggtc acgaacgcgt tcgcgaaccg
     1081 cacgccgcgg gccgcgaggc ggaacttcag gcgcggcatc cgatcggtcg cgtcggcccg
     1141 ctggtcgtcc gtgacgacga ggacgatgtt cgggcgccga gcgtcggtgg gcccggcgac
     1201 cgctcctgca acgacaagag cggctaggag ggctcctgcg gcgggccata tcatggccgt
     1261 agacagtaaa cgtctagttc tgcatagtca agtaacgact attctcaatc taaccgagag
     1321 gcgtatactc cctccgaatg gcgaagaaga acggacggct gggcgagcgg atccgggcgg
     1381 cgcgcgccga gcgcggctgg aagcagaagc acctcgcggc ggaggtctcg gtcgagccga
     1441 tcaccgtctc gcgctgggag cgcggcgcga cgacgccgga cctcgaggtc cttcgcctcg
     1501 tcgccggcgc gaccggcaag ccgctcagct tcttcgtcga cgagggcgcg ccgagcgccc
     1561 aggcctcccc ggggctcgcc gacgcggcga cgcgcatcga ggcggccgcg gggcggatcg
     1621 cggaggagag cgagcggctc gcggtgctcc tctcggagct cgtcgaagag ctacgcgccg
     1681 cgcgctccgg ctcgtaggag gaacgcgtag gccgtgcccg tggcggggcg gcgctccggc
     1741 tccttcgcga gcgcggtcag gagggcggcg gcgagcggcg ccggaaggtc ggcccgcgcg
     1801 gccgccgggt cggcgggctc ctcgagcagg tgcgccctgc acgcggcgac gaccgagcgc
     1861 gcggcggcga agggcggcgc gcccgtcgcg cactcgtacg cgaggcagcc gagcgcgtag
     1921 acgtcggcgc gcgggccgac cggctcgccg cggatcgcct ccggcgcgag gtagtcggcg
     1981 gtgccggcga cccgcccctc ctgcgtgagg acggtgtgcg ccttcgcccg tgcgagcccg
     2041 aagtcgccga gcgcggccgc gccgtcctcc cggagcagga cgttcgacgg cttgacgtcc
     2101 cggtggagga gcccggcgcg gtggagcgcg tcgagcccgg acgcgacgtg ccggacgacc
     2161 cggacgagct cctcccgctc gagcggcccg gctgccagcc ggtcggcgag cgagccgccg
     2221 gcgacgaacg gggcggcgag gtacggacgc ccgtccgcct cgccgtggtc gacgacgcgg
     2281 aggaggtgcg gatggtcgag cgcccgcgcg acctcgctct cgcgctcgaa tcggcgccgg
     2341 tacgacggat cctccgcgag ccccgcgtgc aggaccttga gcgccacctc gccgggcggc
     2401 ccctgcgccc ggaagaccgc gcccacctgg ccgcgcccca gcggctccgc gagcgtgtac
     2461 ggcccgagcc gctcgcccgg ctcggggaac gcggctcctt cgcgcggcgg cggcatcggc
     2521 ggccacctta gtcaccgctg acgcgtctcg cgacgattgc ctctagtcag ggcgccgggc
     2581 gtgtggtacc accgccctat ctcgtcgcga gccagaatcg gcaccgagtt cgccggctac
     2641 cggatcgagg cactcctcgg ccgcggcggg acgagcacgg tctaccgggc cgagagcccg
     2701 cgcctcggcg tgcgcgtcgc gctcaagatc ctgaatccgg agctctccga cgacgagatc
     2761 ttccgcgagc gcttcgtccg ggaggcgcgg gtcgtcgcgc ggatcgagca cccgaacgtg
     2821 atcacgatct acgacgccga cgccttcggc gacgagctct acatcgcgat gcggtacgtg
     2881 ccgggcggag acgtgaccgg cgcgctcgcg gacgggccgc tcgcgtccga gcgcgcggtc
     2941 gcgctgctcg cccaggccgc gagcgggctc gacacggcac acgcccacgg gctcgtccac
     3001 cgggacgtga agccggccaa cctgatgctc gacggcggcg gccgtccggg cgcgcccgag
     3061 acggtgtacg tcacggactt cgggctcacg aagcattcgc agtcgggcag ctcgcccacg
     3121 ccgacgggag tcctcctcgg gacgatcgac tacgtcgcgc cggagcagat cgagggccgc
     3181 tcgcccgacg gccgcgccga cctctactcg ctcgcgtgcg tcgcggtcga gtgcctgagc
     3241 ggccggcggc cgttcgagcg cgacaacgag gcggccgtcc tgtgggcgca catgcaggag
     3301 ccgccgccgt cgctcagcga gcgcgcgccc gagctcccga aaggcgtgga cgagccgatc
     3361 gcgcgcgcgc tcgcgaagaa ccctgacgag cgccagtcga gctgcgcgga gttcgtggag
     3421 gagctgcgcg aggcgctcgc cccgttcggc cggcggccgg cgaaggagtc ggccgcgcgc
     3481 ccggcgccct cgcgggtccg gctcctggca gccgcgctcg caggcgtcgc ggccggcgcg
     3541 gccggcgtcg gcgccgcggt cgtgctcggg aacggcgacg aggcattccc cggcggagcg
     3601 ggacggacgg agacggtcgt ctcgacggtc gtcaccaccg tcacgggccc ggagcccgag
     3661 ctgccgagct tcatcccgca gcagttccgc aaccgctgcc gctcggcgtc gccgccgacc
     3721 gccgacttcg actcgagcta cgtctgcgcc cccggggggg ccgcgtcgac ggtgcgcttc
     3781 agccacgcga tcagcggccc gctgctcgcc tcgtacctcc ggcggcggat ggagcgggtc
     3841 ggcctgcgcg cgccggcgag cgaccggccg atcgtggccg agggctcgtg cgccgagcag
     3901 gtgctgcccg cgatcgagca ctggttcgcg ctcggactcg cgggccacca gtcggccggc
     3961 gagctgccgg gcgcgtccgc cgacgggcgc gtcctctgct acgaggaagg cggggtcgcg
     4021 cacatcgaat ggacgaccgg cgttctcggt gtctatgccc atgcgtccgg gccgcgcttc
     4081 ccgcgcctct accagtggtg gcgcacgaac tccgggccgg tctcgtagcc cgggtctccg
     4141 agtgcgcgtc ggcgcgcgcc gggcccgagg ctgtggacga accggcacga agccggcaca
     4201 cctccggcac agacgcgcgc accggcccgc gatagcgttc gtcctgggag cctcgcgccc
     4261 ccgatggggg cttgcgcgcc ggcgcggccg ccggccggcc cgagcgcgcg cacgacgagg
     4321 tgggggaggc aggattcgaa cctgcgaagg cggagccaac cggtttacag ccggtcccct
     4381 ttggccactc gggaactccc ccggcggacg cggattgtag ccgagcatcc ccgccatcca
     4441 ggctcaaggc gcgcgcgggg gcggccgata cgcggagagg ccatggacct gaacgcactc
     4501 ctcgccgaga cggtcgcgcg cggcggcagc gacctccatc tcaaggtctc ctcgccgccg
     4561 atggcgcgcc tcgacggctc actcgggccg atcgcggaga ccccgctcac ggacgcggac
     4621 ctcgagcgcg cgctcgagat cgtcgccgcc cgcacgccgc agaagctcca gctcttccag
     4681 gagagcggcg acctcgacac cgcgtacacc gcggccgggg tcggccgctt ccgcgtgaac
     4741 ggcttccgcc agcgcggctc gatctcgttc gccttccgcc acgtgccgga cgaggtgccg
     4801 acgttcgaga agctcggcct cccgcgcggc gtcgagctgc tcgcgggcga gcatcgcggc
     4861 ctcgtgctcg tcaccggcgc gaccggctcg gggaagtcga ccaccctcgc cgcgatgatc
     4921 gacaagatca accggacgcg gaagcagcac atcgtgacga tcgaggaccc gatcgagatc
     4981 atccaccgcg atcagagctc gatcgtgaac cagcgcgagg tcggcctcga caccgagtcg
     5041 ttcaagcagg cgctccggcg cgtcctccgg caggaccccg acgtgatcct gatcggtgag
     5101 ctccgcgacg aggagacggc gcagaccgcg ctccaggccg cggagtcggg ccacttcgtc
     5161 ctctcgacga tgcacacggt cgacgcggcc gagacggtcg ggcgcctcat cgagttcttt
     5221 ccgccgacga agcagctgat gctgcggcag atcatcgcgg gcgtcctgcg gggggtcgtg
     5281 agccagcgtc tcctccccgc gaaggacggt ggcaggttgg cagccgtgga ggtgatggtt
     5341 aacactgcac ggatcgccga cctgatccgc gacccgctga agaccgacgg gatcctcgag
     5401 gcgatcgagg agggcgggta ccaccacatg cagagcttct cccagcatct cgtccagctc
     5461 gtcctcgacg aacgcgtcga gttcgaggtc gcggcgacgg ccgcgacgaa ccggcacgac
     5521 ttcgagatcg ccgtccagca ggcggcgcgg ctgaagaacg ccgagagcgc cgccgccgcg
     5581 cagaccccgc cggccgccga gcccgcggcg aacggcgcgc cgcacgtgga ggacgagctg
     5641 gggacgcttc gcctggccgg cccctaggca cgatgctgcg gcgcttcacc ctcctccttt
     5701 ccgccgccct cgtggcggcg ttcgccgtct cgggcgcgca ggccgccgtg ccgagcccgc
     5761 tgcccagcta caccaccccg aatgcgccgg gcgcgatcgt cgtcccgttc tccgtctcga
     5821 ccccgccggc gcggcccgcg cagcgctcgt acgcgcagct cctcggcctc tggcaggagg
     5881 cgggcgccgc gtacggcgtc ccgtgggagg tcctcggggc gatcaacaag atcgagtcga
     5941 acttcgggcg gaacatgggc ccgagctcgg ccggcgcggt cggctggatg cagttcatgc
     6001 cggacacgtg gacgcgctgg gggatggacg gcgacggcga cggcgtcgcg aacccgtggg
     6061 tccccgagga cgcggtctac gccgccgcgc gctacctcgc cgcggccggc gcgcacgacg
     6121 atctctaccg cgcgatcttc gcctacaacc acgcggactg gtacgtgcag gacgtgctcg
     6181 agctcgcggc ggtcttcgcc ggcggcggcg ggttcgaccc gacgctcggc acgggcgcgc
     6241 tcggcttcac cggcggcggc ttcgccggcg gggcggacgc ggtcttccgg atctcggatc
     6301 tcgagacccg gctcgcagat gcgcggcgcg cggtgaagcg ccttcaggag cgcgtctccg
     6361 aggtggagga cctgctcatc gagctcgaca cggccgtctt cgaggcggag cagcgtgcgg
     6421 gcgacccgtc gctctcggac gaggagttcc aggccgtcga gacggaggtg accgggctcg
     6481 tgctccggca ggaggccgag ctcgagcggc tcgccgtccg ccgcgcgcgg ctcgaggagg
     6541 cggcccagct cgtcgacgac ctccgcaacg ccgcgcagga gcaggcgtcg acctacagct
     6601 tcagccgcac gtccgcggtg gacgcgctcg gcgcgccgca cttctccggg cagtacgtct
     6661 tcccggtcgg cggcggcgcg gcggtcgtct cggtcggcgc cgaccaccac gactacccgg
     6721 ccgccgacat cgcggcgccc gagggctcgc cgctctacgc gctcgccgac ggcgtcgtcg
     6781 tggacgtcta cccgacgccg accgggcgct gcgggatcgg gttctccatc cagatcgtgg
     6841 acgggcggac gttcctctac tgccacctct cgtacctcga cccgaccgtc tcgcagggca
     6901 cggcgctcgc ggccgggacc ccggtcggcc tcgtcggctc gaccgggaac tcgaccgggc
     6961 cgcacctcca cctccagctc gtcccctcga gcggcggcta cccgcagcac gagggctggt
     7021 tccagagctt cgccggcgtc gcgttcaggt ggcaggacga ggctccgccg cgcgcccccg
     7081 gggcgcccga cggcgccatc cccttctctc ggtagaggcc ttcccccgtt cggggattga
     7141 ctcgccaccc ctcccgcgcc gatgcaaccc ccatggcggc acacctctct gcgctcctgc
     7201 cccggctggt agcgctcacg ctcatctggc tgctcgcgac ctcgacgatc acgttcgccg
     7261 cgggcggccg caccacgacg ccgccgcctc cgccggccgc gccggcgccg aaggagcccg
     7321 agtacctcgt cgtgcccgac gtgcgccgcc aggcctacgt cttcgcgaag ggcatcctcg
     7381 ccgacggcgg cttcggctgg cgcgtgaccg gcccggtgaa gggctacgcg gccaacacgg
     7441 tcgtctccca gaagccggcc ccgggcgcga aggtcgagga caccggcgct cccgagatcg
     7501 tcctcgtgct cgagcgcaac cccgcctacc ccgagcacgg cctgccggag aacgcctcgc
     7561 cgacccccgg caccccggtc gtcctggccg gtgcgcgcga cctcgcgccc gcgccccagc
     7621 cggagccccc ggtggaggag cagcccgcgg aggagcccgc ccccgcgcag ccggagaagc
     7681 cggagaagcc cgaggagccg aaggacgagc aggccccgaa gaagcagaag caggagcccg
     7741 cggacaagaa gcgcacgccc gcgttccagg tcgacggcgc tcccgccgag ccgctcgacg
     7801 agatccccct gcccgagcgc gcgcggcggc tcgagcagcg cctcgccggg aaggcgaagc
     7861 cgacgccggc gctcgtgaac cactggctct accagcatgc ctgggtcgtc accggtgcgc
     7921 ggttcggctg gagcggcggc gcggaggccc tccggatcct cgtccggacc gaccgcgacc
     7981 tccaggcgcg gtggggcatc ggcgggaaga gcctcgccgt ggctcgggct gcgctggccg
     8041 aggtcgagcg ccgcagcagc cgctgatgcg cgccgggcgc gtcctccggg cgcttcgcgc
     8101 cgcggagggc tacacgatga tcgagctgct cgtcgtgctc atgatcctga cgacggtgct
     8161 gaccgcgctc accgcgctct tcctcgcggg cgcgaactcg gagctcgagt cgaaccgtcg
     8221 cttccaggcc cagcaggacg cgcggctcgc ggccgaccgc ctgcggcgcg aggtgcactg
     8281 cgcgggcggc gcgacggtca gcaccccgtc ctccgtcacc gtccacctgc tcggccactg
     8341 cccgaccgcg atcgggaacg cgccgacgaa cgtcgtctac gacgtcgtgg cggacggcgg
     8401 ccactacgag ctccgacggg ccggcgtccg gatcgccgac cacctcgtga gcagcgaggt
     8461 cttcacgtac accgccccct cggccgcgtc gcgcggcctg ctcggcgtcg acctccaggt
     8521 gaacgtccgc ccgaacgagg gatggaagac gtggcacctc cagaccgaca tcgtcttgag
     8581 gaacacgctg cggcaatgag aattcgcctg agtaactgcg gcaagctgct ccgacgccag
     8641 gacggcatct cgctgatcat ggcgatcgga atcctcggcg tgctctcgct cgccggcacg
     8701 agcacgatct actacgcgag cgcgaacgcc cggagcgccg agtactcgca ggagaacgcg
     8761 agcgcctacg acctggcgga ggcggggatc aacgagatcc tcgccgttct cggcaagccg
     8821 gagaacaacg cgctcgaggc ggacctcctt ccggagacga cccgcacgta cgacgcgggc
     8881 accgtcacgt ggagcggcac gctcgacctc gcgaccgcga cctgggcgat cacgtcgacg
     8941 ggacggatca agaacccgac cggcgcggtc ggcgacgtca cgcggacgct gacggcgaag
     9001 gtgcccgtca ccccgacgta cacccagccg ctcaacaacc cggtctggaa ctacatcttc
     9061 gccacgcgca cgggcacggg cggctgcgac cagacgatga acaacaacgt cggcggcgga
     9121 acgcgcctgt acgtcgccgg gaacctctgc ctctcgaaca acgccgcgat cgagacgagc
     9181 ccgctgatcg tccacgggaa gctctggctc gacgagggac agacccgcgt cggctcgctc
     9241 cagaaccggg tcgaggcgta cgtcggccag ggctgcagga agggccaaaa cctccccgcc
     9301 gtgatcgtcc cctgccccgg cgacagccag gacgtctaca gcatcctcgc cgagaccggc
     9361 atgcccggcg tgaactcgac tccgccgatc atggccgcgc cggtcgtgga ctggctgaac
     9421 tggtaccagg atgcgatgcc gggcccggtg acgccgtgca cgtcgacgaa cggagcggcg
     9481 agcggcgcgg tcccgacctt cgacaacaac gcgatgcgcg acaacagcgt cgggaccgtc
     9541 tacaacctca cgcccgccac cagctacacg tgtcgcgtcg gcccggcgcg tacgccgagc
     9601 ggctgctacc cgccgccggc gccgtccggc acgacccagt gcacggacta ccccgtcggc
     9661 gagatctcct ggaacgcgac gacgcgcatg ctcacggtct acgggacgat ctacctcgac
     9721 gggagcgcgt acgtcggcaa cgggctcctg aaccagtaca acggcgtcgc gacgatctat
     9781 ctctccggga cgctctacat gaacgggaag ctctgcggcg gcgtctcagg gtcgagctgc
     9841 gacttcgcga actgggaccc gaacaaggag ctgatcacgt tcgtcgcggg cgagacgagc
     9901 ccgggcggga cgaacaagcc ggacgtgggc tactcggtcg agctcgagaa caacgcgcag
     9961 ctccagggcg gcctgtacgc gtcggcggcg atcctgctcg ggaacaacgc gaagacggac
    10021 gggccgatgg tcgcgtccta cgtgaccatg tcgaacaacg tcgtcgggag cgacttcccg
    10081 atcatcacga cggttcccgt cgggatgccc ggcaaccccg cggtctacgc ccagccgaac
    10141 gcgccacagc tctacagcgg ctagcggcgc ccgctcgagc cggcaactag taacactgtg
    10201 ttactagctc ggttcacgct cgcccctact ggacgaggac gcggccgagc tcctcgatcg
    10261 acgtgaggcc ggaggcgacc ttcgcgaggc cgtcgtccca gagggtcttc atcccgcctt
    10321 ccatcgcgga gcgctcgatc tcgtcgcggc tcgcgtgcgt cgcggcgagg cggctcaggt
    10381 cctcggtcat gaccatgagc tggtagatgc cgatccggcc cttgtagccg gtctggttgc
    10441 agcgcgggca gcccttcctg cggtagaggg agacgccgtc ggcggcggcc acctgctcgg
    10501 gcgtgaagcg tgcgttcagc atctcctcgt gcgtcgggat gtacatctcg cagcagtggg
    10561 tgcagagctt ccgggcgagg cgctgggcga cgacggcggt gacggcggag ccggtgagga
    10621 acggctcgac gcccatctcg ttcaggcgcg tgagcgcgct cggcgcgtcg ttcgtgtgca
    10681 gggtcgagag gacgaagtga ccggtgaggg cggcctcgat cgccatcttc gccgtctcga
    10741 cgtcgcggat ctcgccgacc atcacgacgt cggggtcgga gcggaggatc gagcggagcg
    10801 cggaggcgaa ggtgaggccg gccttcgggt tcacctgcac ctggttcagc ccgagcatcc
    10861 ggtactcgac cgggtcctcg acggtgatga cgttgatctc cgggcggttc agctgctcga
    10921 gggacgcgta cagcgtcgtc gacttaccgg agcacgtcgg ccccgtcacg aggagcgcgc
    10981 ccgtgggctt gcggatgacc tcctcgagcc gcgtgcgcat ctcgtcggag aagccgaggt
    11041 cggcgagcga cggcagccgc ttcgacttgt cgatcagccg catgaccacc atctcgccct
    11101 cgacggacgg gagcaccgcg acgcggatgt cgagcagccg gcccgccgcc ttcgcgttca
    11161 gcgagatgcg tccgtcctgc ggcttgcgcc gctcggcgat gtcgagcttc gcgagcacct
    11221 tcagccgcgt cgtgaccgcg accgcgagcc gcttcgggat cc
//
LOCUS       DASTFC010000343         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_686391,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000343 DASTFC010000000
VERSION     DASTFC010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686391"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFO88_10130"
     CDS             <1..811
                     /locus_tag="VFO88_10130"
                     /inference="COORDINATES: protein motif:HMM:NF042779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOPRIM nucleotidyl transferase/hydrolase
                     domain-containing protein"
                     /protein_id="HET9545921.1"
                     /translation="FAAALADRDGSAAAPALAFVAGLERWCEAGLSGIVLLVEEPELF
                     LRPQAQRYLHRLLHSFASAGNQVVFSTHSPSFLNVAHLEELAIVGHDPASGTRITQPG
                     PLPADEEFRIVSELDAERSELFLARAALLVEGRTEKLVFPFVFQALGFDADGEAISVI
                     ECGGKSNIPVFAQICDAVGVPFVAVHDRDAEPGKEPIAAESRLNALIETVVGAERVIV
                     LEPDFEGATGVLGHKPQGALRRFADVGPDGLPEPLARAARLVLELAGAARA"
     gene            868..1506
                     /locus_tag="VFO88_10135"
     CDS             868..1506
                     /locus_tag="VFO88_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9545922.1"
                     /translation="MRFRLHYVPGSAAMAPHAALAEAGAGYELRLVERDEQGRSPREY
                     LALNPLGRVPTLEDGDLVLTESAACVLHVAERFPHAHLVPPPGTRERSELYRWLMFMT
                     NTLQPAFLHWFYPERYTVDPAGEEGIRRLAARSIAEHLERLDAELADRPWLVGEERSG
                     ADLFLFMLVRWGRRQDPPAWDRPNLRAHFTRLLDREGVRRMMDEQGLEIPAL"
     gene            1503..2318
                     /locus_tag="VFO88_10140"
     CDS             1503..2318
                     /locus_tag="VFO88_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9545923.1"
                     /translation="MIRRSVAVDGVRASYLTAGVEGPLVLLLHGTYWSRVWAPVLDRL
                     AEAALRPVAVDFPGCGRSEGELTLETAAVPALAGWLERFAEALGAEEPLLVAGHDIGG
                     AVAQHMLAAGGLEVAQLALVNSVTYDSWPVPGVARFRDPEVVAAVTTDELVEARRVAV
                     AKAAARPLTASELEEYLSPWHDLRVGRSWMALAGAADPRYTLDLLPALRESSAPKLLV
                     WGEDDEFQPVTYAERLAAEVPACTLVRVPDAGHIPMENDPDRVAGALAGFFRS"
     gene            complement(2315..2863)
                     /locus_tag="VFO88_10145"
     CDS             complement(2315..2863)
                     /locus_tag="VFO88_10145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET9545924.1"
                     /translation="MRILGISGSLRRDSYNTGLLRTAAQLAPVDVELWDGLKAIPPYD
                     ADDESDPAPAVVELRSALAGADGFLLATPEYNHSVPGHLKNALDWASRPLATNPIRNK
                     PAAVVGASTGAFGAVWAQAELRKVLSAIGARVLEEDLPVGLAATRFDEDGLLADDELR
                     ERLRALVDDLVASVDDRLATAA"
     gene            complement(2872..3411)
                     /locus_tag="VFO88_10150"
     CDS             complement(2872..3411)
                     /locus_tag="VFO88_10150"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9545925.1"
                     /translation="MTTITETIAPAGTWELDPVHSTVAFEVDYLAGTFKGDFRDVAAT
                     FAADGGTARLEGVAQVASIDVKDENLAAHLQSPDFFDAERHPELRFTAEDIALDGDAV
                     TIRGEITMKGVTKPVSLAGTAAPPMTDPYGRERIGLKLATTVDRTEFGIDWNAPLPTG
                     DPALANDVTIVADLHFTRA"
     gene            3490..3972
                     /locus_tag="VFO88_10155"
     CDS             3490..3972
                     /locus_tag="VFO88_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9545926.1"
                     /translation="MSTQLIATEVPPAVRAWTRLMRAHASSVRLLGARLQAEHGLTVN
                     DYEALYVLSRADGGRMKRVDLARRLLLTPSGITRLLEGLERAGLVERQACKEDLRVSY
                     AQLTDAGRERLLAASCAHEGSVRSLMEEHLDADEIEALAEMLAKLPDVAEGDDACPAA
                     "
     gene            4047..4463
                     /locus_tag="VFO88_10160"
     CDS             4047..4463
                     /locus_tag="VFO88_10160"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9545927.1"
                     /translation="MDFAAHVDLARALSASTSLESPRSLAIVESRLRRGDWTPVHLHE
                     TPEAFHVLEGVLAVFAGGEEIELEAGESVVAPSGVPHTVRAPHGPVRYLSGSLVRSAA
                     RYEDFLRAVAPRLDADDALRLSALGASNGISLVAAA"
     gene            4499..>5355
                     /locus_tag="VFO88_10165"
     CDS             4499..>5355
                     /locus_tag="VFO88_10165"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9545928.1"
                     /translation="MAQTEDPRRAGPEPPFPEQAQPAPGRESEMEPRPDYGEETYEGT
                     AKLQDKVALVTGADSGIGRAVALAFAREGADVLVSYLSEDDDAAETVRVVVDAGRKAV
                     PVPGDIGDEEHCKALVERAVDELGRLDILVNNAATQGVHESITEIPSEEIERVFRTNV
                     LSFFWLSRAAIPHLEPGSSIINTTSIQAYDPSPTLIHYAATKGAISTFTKALAKELVE
                     QGIRVNAVAPGPVWTPLIAMSFEPEEVAEFGQDNPLGRPAQPAELAPAYVFLASDEAR
                     FVVGEVIGVT"
BASE COUNT          684 a         1961 c         1958 g          752 t
ORIGIN      
        1 cttcgccgcc gcgctcgccg accgcgacgg gtcggccgcc gcgccggcgc tcgccttcgt
       61 ggccggcctc gagcggtggt gcgaggcagg gctctcgggc atcgtgctcc tcgtggagga
      121 gccggagctc ttcctgcgcc cgcaggcgca gcggtacctc cacaggctcc tccattcgtt
      181 cgcctcggcc gggaaccagg tcgtcttctc gacccactcg ccctcgttcc tgaacgtcgc
      241 gcacctggag gagctcgcga tcgtcggtca cgaccccgcc tcgggaacgc ggatcaccca
      301 gccgggcccg ctgccggccg acgaggagtt ccggatcgtc agcgagctcg acgccgagcg
      361 gagcgagctc ttcctcgccc gcgccgcgct cctcgtcgag gggcggacgg agaagctcgt
      421 cttccccttc gtcttccagg cgctcggctt cgacgccgac ggcgaggcga tctcggtcat
      481 cgagtgcggc gggaagtcga acatcccggt cttcgcgcag atctgcgacg cggtcggcgt
      541 cccgttcgtc gccgttcacg accgggacgc cgagcccggc aaggagccga tcgccgcaga
      601 gagccggctg aacgccctca tcgagaccgt cgtcggcgcg gagcgggtca tcgtgctcga
      661 gccggacttc gagggcgcga ccggcgtgct cggccacaag ccgcaggggg cgctgcgacg
      721 cttcgcggac gtcgggcccg acgggctgcc ggagccgctc gcgcgggcgg cgaggctcgt
      781 gctcgagctg gcaggcgccg cgcgcgcgta gcccgctcac gccgcgggcc gcgcggaccg
      841 gattccgcgg gctaggatcc gcgcggcgtg aggttccggc tccactacgt tcccgggtcg
      901 gcggcgatgg cgccgcacgc cgcgctcgcg gaggccggcg ccgggtacga gctccgcctc
      961 gtcgagcgcg acgagcaggg ccggagcccg cgcgagtacc tcgcgctgaa cccgctcggc
     1021 cgcgtgccga cgctcgagga cggcgacctc gtcctgaccg agtcggccgc gtgcgtcctc
     1081 cacgtcgccg agcgcttccc ccacgcgcac ctcgtcccgc cgccggggac gcgcgagcgc
     1141 tcggagctct accggtggct catgttcatg acgaacacgc tccagccggc cttcctgcac
     1201 tggttctatc ccgagcgcta cacggtcgat ccggccggcg aggaggggat ccggcggctc
     1261 gcggcgcggt cgatcgcgga gcacctcgag cgtctggacg ccgagctcgc ggaccgcccc
     1321 tggctcgtcg gcgaggagcg gagcggcgcc gacctcttcc tcttcatgct cgtccgctgg
     1381 gggcggaggc aggatccgcc cgcctgggac cggccgaacc tgcgcgctca cttcacgcgc
     1441 ctcctcgacc gcgagggcgt ccggcggatg atggacgagc agggactgga gatcccggcc
     1501 ctgtgatccg gcgctcggtc gcggtggacg gcgtccgggc gagctacctg accgccgggg
     1561 tggaaggccc gctcgtgctc ctgctgcacg ggacgtactg gagccgggtc tgggcgcccg
     1621 tcctcgaccg gctcgccgag gcggccctgc ggccggtcgc ggtcgacttc cccggctgcg
     1681 ggcgatcgga gggcgagctc acgctcgaga cggccgccgt gccggcgctc gccgggtggc
     1741 tcgagcggtt cgccgaagcg ctcggcgccg aggagccgct gctcgtcgcc ggccacgaca
     1801 tcggcggcgc tgtcgcgcag cacatgctcg cggccggcgg gctcgaggtg gcgcagctcg
     1861 cgctcgtcaa ctcggtcacg tacgactcct ggccggtgcc cggcgtcgcg cgtttccgcg
     1921 acccggaggt cgtcgccgcg gtgacgaccg acgagctcgt cgaggcgcgc cgcgtcgccg
     1981 tcgcgaaggc ggccgcgcgg ccgctgaccg cctcggagct cgaggagtac ctgagcccct
     2041 ggcacgacct gcgcgtcggc cgctcgtgga tggcgctcgc aggcgccgcg gacccgcgct
     2101 acacgctcga cctcctcccc gcgctgcgcg agtcgagcgc gccgaagctc ctcgtctggg
     2161 gcgaggacga cgagttccag ccggtgacgt acgccgagcg cctcgccgcc gaggtgcccg
     2221 cgtgcacgct cgtccgcgtg ccggacgcgg gtcacatccc gatggagaac gaccccgacc
     2281 gcgtcgcggg cgcgctcgcc gggttcttcc ggagctaggc ggcggttgcg agccggtcgt
     2341 cgacggacgc gacgaggtcg tccacgagcg cgcggagccg ctcgcgcagc tcgtcgtccg
     2401 ccaggagccc gtcctcgtcg aagcgcgtcg ccgcgagccc gaccggcagg tcctcctcga
     2461 ggacgcgcgc gccgatcgcc gacagcacct tgcggagctc cgcctgcgcc cagaccgcgc
     2521 cgaacgcgcc ggtcgaggcg ccgacgaccg cggccggctt gttgcggatc gggttcgtgg
     2581 cgagcgggcg cgacgcccag tcgagcgcgt tcttcaggtg acccgggacg gagtggttgt
     2641 actccggcgt ggcgaggagg aacccgtcgg cgccggcgag cgcggagcgg agctccacga
     2701 ccgccggtgc cggatccgac tcgtcgtccg cgtcgtaggg cgggatggcc ttgaggccgt
     2761 cccagagctc gacatcgacg ggcgcgagct gcgccgccgt gcggaggagg cccgtgttgt
     2821 acgagtcccg ccgcaggctg ccggagatgc cgaggatccg cacgtcgctc cctacgccct
     2881 cgtgaagtgg aggtcggcga cgatcgtgac gtcgttcgcg agcgccgggt cgccggtggg
     2941 gagcggggcg ttccagtcga tcccgaactc cgtccggtcg accgtcgtcg cgagcttcag
     3001 gccgatgcgc tcgcgcccgt acgggtccgt catcggcgga gcggccgtgc cggcgaggga
     3061 gaccggcttc gtgacgccct tcatcgtgat ctcgccgcga atcgtgacgg cgtcgccgtc
     3121 gagcgcgatg tcctccgccg tgaagcgaag ctccggatgg cgctcggcgt cgaagaagtc
     3181 cggggactgg aggtgggcgg cgaggttctc gtccttcacg tcgatgctcg cgacctgcgc
     3241 cacgccctcg aggcgcgcgg tgccgccgtc ggcggcgaac gtcgccgcga cgtcgcggaa
     3301 gtcgcccttg aacgtgccgg cgaggtagtc gacctcgaaa gccaccgtcg agtggacggg
     3361 gtcgagctcc caggtgccgg ccggagcgat ggtctcggtg atcgtcgtca tggctttcgt
     3421 cctttcgttg cgtggtgtaa tattgagtac gcaatcaaat gtagcacgct aagattgaga
     3481 gcgcaaacaa tgtccaccca gctgatcgca accgaggtac cgccggcagt ccgcgcgtgg
     3541 acgcggctca tgcgcgctca cgcgtcgtcc gtcaggctgc tcggcgcacg gctccaggcc
     3601 gagcacgggc tgaccgtcaa cgactacgag gcgctgtacg tcctctcccg tgcggacgga
     3661 ggccggatga agcgcgtcga cctcgcgcgc aggctcctgc tcacgccgtc cgggatcacc
     3721 cggctcctcg aggggctcga gcgcgccggg ctcgtcgagc ggcaggcgtg caaggaggac
     3781 ctccgcgtct cgtacgcgca gctcacggac gcggggcggg agaggctcct cgcggcgtcg
     3841 tgcgcgcacg agggatcggt gcgctcgctc atggaggagc acctcgacgc ggacgagatc
     3901 gaggcgctcg ccgagatgct cgccaagctc cccgacgtcg ccgagggcga cgacgcctgc
     3961 ccggccgcct gaggttcggc tagccgaacg caaggacgcg gggtggcctc attccgctgc
     4021 gggccgcggc acgcgaggct tcgatcatgg acttcgcggc acacgtcgac ctggcccgcg
     4081 ccctctccgc ctcgacctcg ctcgagtcgc cgcggagcct cgcgatcgtc gagagccgcc
     4141 tccggcgcgg cgactggacg cccgtgcacc tgcacgagac gcccgaggcg ttccacgtgc
     4201 tggagggcgt gctcgcggtc ttcgccggcg gcgaggagat cgagctcgag gccggcgaga
     4261 gcgtcgtcgc cccgtccggc gtcccgcaca cggtgcgggc cccccacggg ccggtgcgct
     4321 acctgagcgg ctcgctcgtc cgctcggcgg cccgctacga ggacttcctt cgcgccgtcg
     4381 cgccgcgcct ggacgccgac gacgccctcc ggctctccgc gctcggggcg tccaacggca
     4441 tctcgctcgt cgccgccgcc taggtttccg ccccgcccgc ccgggaacgc gggggttcgt
     4501 ggcgcagacc gaggatccaa ggcgggccgg ccccgagccg ccgttcccgg agcaggcgca
     4561 gcctgcgccc gggcgcgaga gcgagatgga gcctcgcccg gactacggcg aggagacgta
     4621 cgagggcacg gcgaagctcc aggacaaggt cgcgctcgtc acgggcgccg acagcggcat
     4681 cggccgcgcg gtcgcgctcg cgttcgcccg tgagggcgcc gacgtgctcg tctcgtacct
     4741 ctccgaggac gacgacgccg ccgagacggt tcgcgtcgtc gtggacgcag gccgcaaggc
     4801 cgtgcccgtt cccggcgaca tcggggacga ggagcactgc aaggcgctcg tcgagcgcgc
     4861 cgtggacgag ctcggacggc tcgacatcct cgtgaacaat gcggcgacgc agggtgtgca
     4921 cgagagcatc acggagatcc cgtcggagga gatcgagcgc gtcttccgga cgaacgtcct
     4981 cagcttcttc tggctctccc gcgccgcgat cccccatctc gagcccggct cctcgatcat
     5041 caacacgacg tccatccagg cgtacgaccc gtcgccgacg ctcatccact acgcggcgac
     5101 gaagggcgcg atctcgacgt tcacgaaggc gctcgcgaag gagctcgtgg agcagggcat
     5161 ccgcgtgaac gccgtcgcgc cggggccggt ctggacgccg ctcatcgcga tgtccttcga
     5221 gccggaagag gtcgcggagt tcggtcagga caacccgctc gggcgtccgg cgcagccggc
     5281 cgagctcgca cccgcctacg tcttcctcgc gtccgacgag gcgcgcttcg tcgtcggcga
     5341 ggtgatcggc gtgac
//
LOCUS       DASTFC010000344         5145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1346056,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000344 DASTFC010000000
VERSION     DASTFC010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346056"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFO88_10170"
     CDS             complement(<1..269)
                     /locus_tag="VFO88_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545929.1"
                     /translation="MRDATVLVVNAGSTSLKLHLVSPADDAEALASFRDVAPGRVAAV
                     AHRVVHGGELREPARLTDDVLAAIEDASTLAPLHNRPALAAIDEAV"
     gene            complement(277..600)
                     /locus_tag="VFO88_10175"
     CDS             complement(277..600)
                     /locus_tag="VFO88_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545930.1"
                     /translation="MQRIAVIAKLKEGAAERAHELLAEGPPFDPDALGFDRHAVYVSD
                     TEVVFIFEGARVGELIRTLAAADADAEHVFSAWEPLLDGLPRLGQEAFFWQRPAPVEA
                     GAWGE"
     gene            706..1446
                     /locus_tag="VFO88_10180"
     CDS             706..1446
                     /locus_tag="VFO88_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9545931.1"
                     /translation="MEERWTDLDGLRLYARVWERPDAPQLVLVHGLAVSSRYFVPLAE
                     ELAASFSVLAPDLPGFGRSTKPRRTLSVTELARALAAWLDANGVERPVLVANSLGCQF
                     AVELAARSPERVAVLVLIGPALEPRTRSRTRWVARWLATAFLERPPLHLVVALDALRA
                     GPVRVWATFEDALRNPIDGELGRVSAPVLVLRGEHDPIAPQAACERMAAALPNGRLAV
                     VPGAGHALNWSRPRDAARAIRAWLEEAE"
     gene            complement(1390..2184)
                     /locus_tag="VFO88_10185"
     CDS             complement(1390..2184)
                     /locus_tag="VFO88_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9545932.1"
                     /translation="MRSSPARKRSARPELREAAVDGVRVSYRVAGAGPPLVCVHGLAG
                     SSRWWEPVVPALEERRSVYLGDLPGFGGLGRRTRRVPLAAAAGWLVAWADAVGLRRFG
                     LAGHSMGAAIAVRVAAERPERVEQLVLVAPAGLHLRPLVGHGLPLASTLRRSGPRLLW
                     LLGRDVARAGPRTLVRATLEVAADDVRGELGRVRAPTLVVCGERDALVPPSVGELVRG
                     ELPDAELAVVAGAAHVPMVERPDACAKALLGFLEPGPDRAGGVTGT"
     gene            complement(2144..2650)
                     /locus_tag="VFO88_10190"
     CDS             complement(2144..2650)
                     /locus_tag="VFO88_10190"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9545933.1"
                     /translation="MRALLHGPRGHPVHPPLTDATIGAYTAATALAVLAAFGVAEDKL
                     APAFWITLVVGLVFNAPTSVTGLLDWLEIKPGTPLKRTATTHLLIMVAAAVLFGLAAI
                     FGYGAWSDGELDAFPLVLTLLGFGTLTAGGWIGGSLVFVHGMRVEEEPEKPARDAIVP
                     GEEEIREA"
     gene            complement(2681..>5145)
                     /locus_tag="VFO88_10195"
     CDS             complement(2681..>5145)
                     /locus_tag="VFO88_10195"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET9545934.1"
                     /translation="LLPRLTPELQARIPANLLRLYCPGETLADALPNLRAVYCGTMAY
                     QIEHISDHEERVWLRRAIESGAYRQPLSSDEKRALLTRLSEVEGFEGYLRRAFLGQKQ
                     FSIEGLDAMVPMLDEAIEVASAAGADDVVMGMAHRGRLSVIAHVLGRPYDVILREFEG
                     ERLLEAVTYFAEGGTGDVKYHYGSEGTRTTGNGDVDVHLASNPSHLEAVNPVVEGTTR
                     ALQTDHDSPEGTHDPRVALPVLIHGDAAFPGQGIVAETMNLQDLRGYSTGGTLHIIAN
                     NQVGFTTDPDEGRSTRYSSDIAKGYDSPIIHVNADDAEATIAAIRLAMGYRERFGRDV
                     VVDLVGYRRFGHNEGDEPAYTQPLMYEKIEDHPSVRALYARQLAEEGVVTEEEAEGLV
                     TEVQRRIKEAHEALKEAIAEGGSGARRERRPGRVGTEEPVTAIPLERLEELNRLLLEV
                     PEGFEVHPKLRKQLERRLDSLGESGGIDWAQAEAFALGSLVTEGTPVRLTGQDTERGT
                     FAHRHLVLHDVETGQKYAPIQHLPGAKAPFEVYNSPLSEAGCVGFEYGYAGACQNALV
                     LWEAQFGDFVNGAQVIIDQFVVASLSKWGQTSRLTFLLPHGYEGNGPEHSSARVERFM
                     QMAAQENIRIVNCTTAAQYFHLLRRQALHGKARPLVVLTPKSLLRLKQATSHAVELAE
                     GRFQPILDDPTLDAEQRARTQRLVLCTGKVYYDIVGHAARGEAEGTAVARLEQLYPFP
                     VAAYDQLLSRYPRVTEVVWTQEEPQNMGAWRAIRHRLEEGLPSDVHLRYCGRPWRASP
                     SEGYPTAHLVEQDRIVNEALRA"
BASE COUNT          652 a         1931 c         1872 g          690 t
ORIGIN      
        1 acggcctcgt cgatcgccgc gagggcgggg cggttgtgga gcggcgcgag ggtggaggcg
       61 tcctcgatcg ccgcgagcac gtcatccgtg agccgcgccg gctcccggag ctcgccgccg
      121 tgcacgaccc ggtgcgccac cgccgcgaca cggcccgggg cgacgtcccg aaacgacgcg
      181 agcgcctccg cgtcgtcggc gggcgagacg aggtggagct tcaggctcgt ggagcccgcg
      241 ttgacgacga ggactgtcgc gtcccgcacc ccggggctat tccccccagg ctccggcctc
      301 gaccggcgcc ggcctctgcc agaagaacgc ttcctgcccg agccgcggca ggccgtcgag
      361 gagcggctcc caggcggaga acacgtgctc ggcgtcggcg tccgcggcgg cgagcgtccg
      421 gatgagctcc ccgacacgcg caccctcgaa gatgaagacg acctcggtgt ccgacacgta
      481 gaccgcgtgc cggtcgaagc cgagcgcgtc cgggtcgaac ggcggcccct cggcgaggag
      541 ctcgtgcgcc cgctcggcgg ccccctcctt cagctttgcg atgactgcga tgcgctgcat
      601 cgcccctcct ttccgtgggg tcgcggccat cctcgccgac ggcgccgacc ggggcatcgt
      661 ggcagtcgcg gaagccggcg cgggaaagcc gcaagactgc ctcgcgtgga ggagcgctgg
      721 acggacctcg acgggctgcg gctgtacgcg cgcgtctggg agcgcccgga cgcgccgcag
      781 ctcgtgctcg tgcacgggct cgccgtctcg agccgctact tcgtgccgct cgcggaggag
      841 ctcgccgcgt cgttctccgt cctcgcgccc gacctgcccg gcttcggccg cagcacgaag
      901 ccgcgccgga cgctctccgt caccgagctc gcccgcgcgc tcgcggcctg gctcgacgcg
      961 aacggcgtcg agcggcccgt gctcgtggcc aactcgctcg gctgccagtt cgccgtggag
     1021 ctcgccgcgc gctcgccgga gcgcgtcgcc gtgctcgtcc tcatcggccc cgcgctcgag
     1081 ccgcgcacgc gcagccggac gcgctgggtc gcacgctggc tcgcgaccgc gttcctcgag
     1141 cgcccgccgc ttcacctcgt cgtcgcgctc gacgcgctcc gcgccgggcc cgtgcgtgtc
     1201 tgggcgacgt tcgaggacgc gctccgcaac ccgatcgacg gcgagctcgg ccgggtctcg
     1261 gcgccggtgc tcgtcctgcg cggcgagcac gatccgatcg ccccgcaggc ggcgtgcgag
     1321 cggatggcgg ccgcgcttcc gaacgggcgg ctcgccgtcg tcccgggcgc ggggcacgcg
     1381 ctcaactggt cacgtccccg tgacgccgcc cgcgcgatcc gggcctggct cgaggaagcc
     1441 gagtagcgcc tttgcgcacg cgtccggacg ctcgaccatc gggacgtgcg ccgcgcccgc
     1501 gacgacggcg agctccgcgt ccggcagctc cccccgcacg agctcgccga ccgagggcgg
     1561 gacgagcgcg tcccgctcac cgcagacgac gagcgtgggc gcgcggacgc ggccgagctc
     1621 gccgcggacg tcgtcggcag ccacctcgag cgtcgcgcgg acgagcgtcc gcgggccggc
     1681 gcgcgcgacg tcgcgcccga gcagccagag gagccgcggg ccgctccgcc gcagggtcga
     1741 ggcgagcggc agcccgtgcc cgacgagcgg gcgcaggtgg aggccggccg gagcgacgag
     1801 aacgagctgc tccacgcgct cgggccgctc ggcggccacg cggaccgcga tcgccgcgcc
     1861 catcgagtgc ccggcgaggc cgaaccgccg aaggccgacg gcgtccgccc aggcgacgag
     1921 ccacccggcc gctgcggcga gcggcacgcg ccgcgtccgc cgcccgaggc cgccgaaccc
     1981 gggcaggtcg ccgaggtaca ccgaacgccg ctcctcgagc gcgggcacga ccggctccca
     2041 ccagcgactc gaccccgcga gcccgtgcac gcagacgagc ggcggccctg cgcccgcgac
     2101 gcggtacgag acacggacgc cgtcgaccgc cgcctcgcgg agctcaggcc tcgcggatct
     2161 cttcctcgcc ggggacgatc gcatccctgg ccggcttctc cggctcctcc tcgacccgca
     2221 tcccgtgcac gaagacgagc gagccgccga tccacccgcc cgccgtcagc gtcccgaagc
     2281 cgagcagcgt gagcacgagc gggaaggcgt ccaactcgcc gtccgaccag gcgccgtagc
     2341 cgaagatcgc ggcgagcccg aagaggacgg ccgccgcgac catgatcagg aggtgcgtcg
     2401 tcgccgtgcg cttgagcggc gtgccgggtt tgatctcgag ccagtcgagc aggcccgtga
     2461 cgctcgtggg cgcgttgaag acgagcccga cgacgagcgt gatccagaag gcgggcgcga
     2521 gcttgtcctc cgcgacgccg aacgccgcga gcacggcgag cgcagtcgcc gccgtgtacg
     2581 ccccgatcgt cgcgtcggtg agcggcgggt ggacggggtg cccgcgcggc ccgtgcagga
     2641 gcgccctcat gcacgctcgc ctacccgcga gcgcccgagg ctacgccctg agcgcctcgt
     2701 tcacgatccg gtcctgctcc acgaggtgcg cggtcggata gccctcgctc gggctggcgc
     2761 gccacggccg gccgcagtag cggaggtgca cgtcggacgg cagcccctcc tcgagccggt
     2821 gccggatcgc ccgccaggcg cccatgttct gcggctcctc ctgcgtccac acgacctcgg
     2881 tcacgcgcgg gtagcgcgag aggagctggt cgtacgccgc gacggggaac gggtagagct
     2941 gctcgagccg cgcgaccgcg gtcccctccg cctcgccgcg cgccgcgtga ccgacgatgt
     3001 cgtagtagac cttcccggtg cagaggacga gccgctgggt gcgcgcccgc tgctcggcgt
     3061 cgagcgtcgg atcgtcgagg atcggctgga agcgcccttc ggcgagctcg accgcgtgcg
     3121 aggtcgcctg cttcaggcgc aggaggctct tcggcgtcag cacgacgagc ggccgcgcct
     3181 tcccgtgcag cgcctggcgg cggagcaggt ggaagtactg cgccgcggtc gtgcagttga
     3241 cgatgcggat gttctcctgc gccgccatct gcatgaaccg ctcgacgcgt gcgctcgagt
     3301 gctcggggcc gttcccctcg tagccgtgcg gaaggaggaa cgtgagccgc gacgtctggc
     3361 cccacttcga gagcgaggcg acgacgaact ggtcgatgat cacctgcgcg ccgttcacga
     3421 agtcgccgaa ctgggcctcc cagaggacga gcgcgttctg gcaggcgccg gcgtagccgt
     3481 actcgaaccc gacgcacccg gcctcggaga gcggcgagtt gtagacctcg aacggcgcct
     3541 tcgcaccggg caggtgctgg atcggcgcgt acttctgccc cgtctcgacg tcgtgcagca
     3601 cgaggtggcg gtgcgcgaac gtgccgcgct ccgtgtcctg gcccgtgagc cgcaccggcg
     3661 tcccctcggt gacgaggctg ccgagcgcga aggcctcggc ctgcgcccag tcgatcccgc
     3721 ccgactcgcc gagcgaatcg agccgccgct cgagctgctt gcggagcttc gggtgcacct
     3781 cgaagccctc gggcacctcg aggaggagcc tgttcagctc ctcgagccgc tcgagcggaa
     3841 tcgccgtgac tggctcctcc gtgcccaccc gcccgggccg ccgctcgcgc cgcgcgccgg
     3901 agccgccctc ggcgatcgcc tccttgagcg cctcgtgcgc ctccttgatc cggcgctgca
     3961 cctcggtcac gaggccctcg gcctcctcct cggtgacgac gccctcctcg gcgagctgcc
     4021 gcgcgtacag cgcgcgcacg ctcgggtggt cctcgatctt ctcgtacatg agcggctgcg
     4081 tgtacgccgg ctcgtcgccc tcgttgtggc cgaagcgccg gtagccgacg aggtcgacga
     4141 cgacgtcgcg gccgaacctc tcgcggtagc ccatcgcgag ccggatcgcg gcgatcgtcg
     4201 cctccgcgtc gtcggcgttc acgtggatga tcggcgagtc gtagcccttg gcgatgtcgc
     4261 tcgagtagcg cgtcgagcgc ccctcgtccg ggtcggtcgt gaagccgacc tggttgttcg
     4321 cgatgatgtg gagcgtcccg ccggtcgagt agccgcggag atcctggagg ttcatcgtct
     4381 cggcgacgat cccctggccg gggaacgccg cgtcgccgtg gatcagcacg gggagcgcga
     4441 cgcgcgggtc gtgcgtcccc tcgggcgagt cgtggtcggt ctggagggcg cgggtcgtcc
     4501 cctcgacgac cgggttcacg gcctcgaggt ggctcgggtt cgacgcgagg tgcacgtcca
     4561 cgtcgccgtt cccggtcgtc cgcgtcccct cggagccgta gtggtacttg acgtcgccgg
     4621 tgccgccctc ggcgaagtac gtgacggcct cgagcaggcg ctcgccctcg aactcgcgca
     4681 ggatcacgtc gtacgggcgc ccgagaacat gcgcgatcac gctgagccgt ccgcggtggg
     4741 ccatgcccat gaccacgtcg tccgcgccgg cggcggaggc gacctcgatc gcctcgtcga
     4801 gcatcggcac catcgcgtcg agcccctcga tcgagaactg cttctgcccg aggaacgcgc
     4861 ggcggaggta cccctcgaag ccctcgacct cggagagccg cgtgaggagc gcgcgcttct
     4921 cgtcggaaga gagcggctgc cggtaggcgc cggactcgat cgcccgcctg agccagaccc
     4981 gctcctcgtg atccgagatg tgctcgatct ggtacgccat cgtcccgcag tagacggcgc
     5041 ggaggttcgg cagcgcgtcg gcgagcgtct cgccggggca gtagaggcgc aggaggttcg
     5101 cggggatccg ggcctggagc tcgggcgtga ggcgcggcag gagct
//
LOCUS       DASTFC010000345         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_439846,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000345 DASTFC010000000
VERSION     DASTFC010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439846"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFO88_10200"
     CDS             <1..207
                     /locus_tag="VFO88_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545935.1"
                     /translation="AETKDVAEEELGDELDAFDASLQALRADFDAAETEGFSGASLSA
                     LVTSVSNALAAFEALIAAAPDCDL"
     gene            complement(238..447)
                     /locus_tag="VFO88_10205"
     CDS             complement(238..447)
                     /locus_tag="VFO88_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HET9545936.1"
                     /translation="MDTPSAIAWFEGLAFPLVSLGVLVVWAYTLTDLWKNPSLSGSAK
                     AMWMLIIVLLPILGPVVYLTVREDW"
     gene            complement(550..966)
                     /locus_tag="VFO88_10210"
     CDS             complement(550..966)
                     /locus_tag="VFO88_10210"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET9545937.1"
                     /translation="MHLIADGDRDRASEALRGHYQRGRLSLDELAERLDVALSARTSR
                     ELRTAFRGLPSPWSPGELASTFEGATRAASRVLLFLALAGVWSMASLFLLVAFVVVLA
                     AGASAAAQVAVPVLWIAVSYLAWRAWKRPSHARTRG"
     gene            complement(1005..1096)
                     /locus_tag="VFO88_10215"
     tRNA            complement(1005..1096)
                     /locus_tag="VFO88_10215"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1062..1064),aa:Sec,seq:tca)
     gene            complement(1491..>2355)
                     /locus_tag="VFO88_10220"
     CDS             complement(1491..>2355)
                     /locus_tag="VFO88_10220"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HET9545938.1"
                     /translation="LAPIPDGARVQVGHGTAEHHGRVVRAGGRWAQLRLASPAVAARG
                     DRVILRGETTLGGGTVLDPSPPRHASAERFERLERGDLAALVHAPVRAASLAHLADGA
                     PDGLARAGDWLFAPAWLEELRERLAARIAAADPLEPGIPAPSEPWARDIVPLLGLERR
                     GSRLYLPEAAPALEGARAEAADRLRADLAAARFEPVKVEDARLAAFLEEAGDLVRVGD
                     GLALGADAFAEARRLVVEECEGAGSITLARLRDRLGTGRRPAQLILERLDADGVTRRV
                     GDERVLRRKNR"
BASE COUNT          327 a          890 c          866 g          272 t
ORIGIN      
        1 gccgagacga aggacgtcgc ggaggaggag ctcggagacg agctcgacgc gttcgacgcg
       61 tccctccagg cgctccgggc cgacttcgac gccgccgaga cggaggggtt ctcgggcgcg
      121 tcgctctccg cgctcgtcac ctcggtctcg aacgcgctcg ccgcgttcga ggcgctgatc
      181 gcagccgcgc cggactgcga tctgtaggtc accgtcccgc gcccggcacc cggcgggcta
      241 ccagtcctcg cgaaccgtga ggtagacgac cgggccgagg atcggcagga ggacgatgat
      301 cagcatccac atcgccttcg ccgagccgga caggctcggg ttcttccaca gatcggtgag
      361 ggtgtacgcc cagacgacca gcaccccgag gctgacgagc gggaaggcga ggccctcgaa
      421 ccacgcgatg gcggacggcg tgtccattgc cggaccgtgt cagccgtgtg tccgcgcgtc
      481 ttcacccccg ccgcggttca cccaggtgat caggccacgg cgcctcttgg acggcgggtc
      541 acgctggccc tagccccggg tgcgtgcgtg cgagggccgc ttccacgccc gccaggcgag
      601 gtacgagacc gcgatccaga ggacgggaac ggcgacctgc gccgcggccg acgctccggc
      661 cgcgagcacg acgacgaacg cgacgaggag gaagaggctc gccatgctcc agacgccggc
      721 gagcgcgagg aagagcagaa cccgcgaggc ggctcgcgtg gccccttcga acgtggaggc
      781 gagctcgccc gggctccacg gcgagggaag gccacggaac gcggtacgaa gctcccggct
      841 ggttcgcgca ctgagcgcga catcgagccg ctccgcgagc tcgtccaggc tgagccggcc
      901 cctctggtaa tggccgcgaa gcgcctccga cgcgcggtcg cggtcaccgt ccgcgatcag
      961 atgcattccc gtcatcctag cccggccgga aggcctggca gcgacggagc ggccccggag
     1021 tcgaaccgag ccagcgacgg gttgcgccgc ctcaccggtt ttgaagaccg gctgggccac
     1081 cgggcccggc cgctccgccg ccgagggtag caccgcacag gcccgttaaa tacagcgggg
     1141 gcgggccgcc gcagcccgcc cccgcacctg cttctccagt cgagcccccg ccgcatcggg
     1201 gcccggatct gtgtgagccg cagtgccgta tccccagtat cgccgcgggt ggccgcttga
     1261 ttccatgcct cgggaggtgg ttcaccgcgc cgcttcactt ttcccccttc tggggtaggc
     1321 gaggacccgc ggagtcccgc atagtgcgac tcgccgggga cgtgaggggc gaaaatcccg
     1381 gccaagcggg gcagtaggag gccgcagcct actgacccgc gaggggcggc aatagccgcc
     1441 cctcagctat ttaacgagcc cgccgcaggc tctgcgagcc cggacgctcc ttaccggttc
     1501 ttccggcgca gcacgcgctc gtcgccgacg cgccgcgtga cgccgtcggc gtcgagccgt
     1561 tccaggatga gctgcgcggg ccgccggccg gtgccgagcc tgtcgcggag gcgcgcgagc
     1621 gtgatcgagc ccgcgccctc gcactcctcg accaccaggc gccgcgcctc ggcgaacgcg
     1681 tccgcgccga gcgcgaggcc gtcgccgacg cggacgaggt cgccggcctc ctcgagaaac
     1741 gctgcgagtc tcgcgtcctc caccttcacc ggctcgaagc gggccgcggc cagatcggcg
     1801 cgcaagcggt ccgccgcctc ggcgcgcgcg ccctcgagcg ccggggccgc ctccggcagg
     1861 tagaggcggg agccgcgccg ctccaggccg aggagcggga cgatgtcgcg cgcccacggc
     1921 tccgaggggg ccgggatccc gggctcgagc gggtcggcgg ccgcgatccg ggccgcgagc
     1981 cgctcgcgca gctcctcgag ccacgcgggc gcgaacagcc agtccccggc gcgcgcgaga
     2041 ccgtccggcg cgccgtccgc gaggtgggcg agggacgcgg cgcggacggg cgcgtggacg
     2101 agggccgcga ggtctccgcg ctcgagccgc tcgaagcgct ccgccgacgc gtgccgcggc
     2161 ggcgacgggt cgaggacggt gccgccgccg agcgtcgtct cgccgcgcag gatcacccgg
     2221 tcgccgcgcg ccgcgaccgc cggcgaggcg agccggagct gcgcccaccg cccgcccgcc
     2281 cggacgaccc gcccgtggtg ctccgccgtc ccgtggccga cctgcacacg cgcgccgtcc
     2341 gggatcggag cgagc
//
LOCUS       DASTFC010000346         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1313434,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000346 DASTFC010000000
VERSION     DASTFC010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313434"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFO88_10225"
     CDS             <1..419
                     /locus_tag="VFO88_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545939.1"
                     /translation="AHAALGGAYVRHPSHGGARPFLEALRRIAAHLPIERSRMDEQTV
                     REAAEIHARATVERDYPTAGSYLGDGMMDAAGAVMKEMPRPLTGSEVTSVEETEGGYT
                     CRIRYTGESGATDVDSHWADMDGSPTIVGLEVVEKS"
     gene            424..906
                     /locus_tag="VFO88_10230"
     CDS             424..906
                     /locus_tag="VFO88_10230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9545940.1"
                     /translation="MPVDVRPVEERDRGWLREAVAGAWASDRMVTRGRLIEPVSACPG
                     FVAELDGRPAGLVLFAEREDGLEVVGLLALERGAGVGSALLAALEGEAARRGTRAWLV
                     TTNDNTRALRFYQRRGWELVALHREAVTEARRLKPEIPETGDDGIPIRHELELRWTGG
                     "
     gene            956..1390
                     /gene="rplM"
                     /locus_tag="VFO88_10235"
     CDS             956..1390
                     /gene="rplM"
                     /locus_tag="VFO88_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9545941.1"
                     /translation="MKTWNAKAEDVEREWFVVDAEGQTLGRLATRIADTLRGKRKPTY
                     TPHVDTGDFVVVVNAEKIRVTGNKLDQKRYYRHSGYPGGLRSRTLREQLDRQPAEVIR
                     KAVKGMLPRNRLANRQITKLKVYAGPNHPHEAQNPKPLPEDK"
     gene            1387..1782
                     /gene="rpsI"
                     /locus_tag="VFO88_10240"
     CDS             1387..1782
                     /gene="rpsI"
                     /locus_tag="VFO88_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9545942.1"
                     /translation="MSELAQYRGTGKRKTSVARVILRPGDGQWWVNGKTLEEYFPRAS
                     QRMVATAPLRVAESEGRFDVRVRLHGGGPSGQAGALRHGLARALVEADPALRTALKRE
                     GFLTRDARIVERKKAGLHKARKAPQYSKR"
     gene            1933..>2673
                     /locus_tag="VFO88_10245"
     CDS             1933..>2673
                     /locus_tag="VFO88_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9545943.1"
                     /translation="MGDGRRYFGTDGVRGVVGDSLTEDLVARVGFAFAQWSDAAPVLV
                     GRDTRASGPALEAALARGLAAAGSEVTLGGVLPTPAVALLADGCGAVVSASHNPPEYN
                     GVKLFAREGRKLTDEDEALVEGLLPEQVPTVDAEPRRDDTARDRYLELVTERFGAPLD
                     GLRIAVDTANGAMSGLAEEAFERLGARVTALGNAPDGTNINVGCGATDPAALAAGTRA
                     SEHDLGVALDGDGDRVVAVDAAGNEVNGD"
BASE COUNT          388 a          955 c         1008 g          322 t
ORIGIN      
        1 tcgcgcacgc ggcgctcggc ggggcgtacg tccgccatcc ctcgcacggc ggggcgcggc
       61 ccttcctcga agccctgcgt agaatcgccg cgcacctacc gatcgagagg agccgcatgg
      121 acgagcagac ggtgcgcgag gcggcggaga tccacgcccg cgccacggtg gagcgcgact
      181 acccgacggc cgggtcgtac ctcggcgacg ggatgatgga cgccgccggc gccgtgatga
      241 aggagatgcc gcgcccgctc acgggctcgg aggtgacctc cgtcgaggag acggaaggcg
      301 gctacacgtg ccggatccgc tacacgggcg agagcggcgc gaccgacgtc gactcccact
      361 gggcggacat ggacggcagc ccgacgatcg tcggcctcga ggtcgtcgag aagtcctgag
      421 gccgtgccgg tcgacgtccg gccggtcgag gagcgggatc gcgggtggct gcgcgaggcg
      481 gtcgccggcg cctgggcgtc cgaccggatg gtcacccgcg ggcggctgat cgagcccgtc
      541 tcggcgtgtc ccgggttcgt cgcggagctc gacgggcgcc cggccgggct cgtgctcttc
      601 gccgaacgag aggacgggct cgaggtcgtc ggtctcctcg cgctcgagcg gggcgccggc
      661 gtcgggagcg cgcttctcgc ggcgctcgag ggcgaggcgg cgcgccgcgg cacgcgcgcc
      721 tggctcgtca cgacgaacga caacacgcgc gcgctccgct tctatcagcg ccgcggctgg
      781 gagctcgtcg cgctccaccg cgaggcggtg acggaggcgc ggcggctcaa gccggagatc
      841 ccggagacgg gcgacgacgg catcccgatc cggcacgagc tcgagctccg gtggacgggc
      901 gggtgactcc tgctaccatg cgccctcggc ggacacgcgt ccgccgcttc tttgcatgaa
      961 gacctggaac gcgaaagccg aggacgtcga gcgcgagtgg ttcgtcgtcg acgccgaggg
     1021 ccagaccctc gggcgtctcg ccacgcgcat cgccgacacg ctgcgcggca agcgcaagcc
     1081 gacgtacacc ccgcacgtcg acaccggtga cttcgtcgtc gtcgtgaacg ccgagaagat
     1141 ccgggtgacc ggaaacaagc tcgatcagaa gcgctactac cgccactcgg gctaccccgg
     1201 cggcctgcgc tcgcggacgc tccgcgagca gctcgaccgc cagccggccg aggtgatccg
     1261 caaggcggtc aaggggatgc ttccgcgcaa ccgcctcgcg aaccggcaga tcacgaagct
     1321 caaggtctac gccggcccga accacccgca cgaggcccag aacccgaagc cgcttccgga
     1381 ggacaagtga gcgagctcgc ccagtacagg ggcaccggca agcggaagac ctccgtcgcc
     1441 cgcgtgatcc tccggcccgg cgacgggcag tggtgggtga acgggaagac gctcgaggag
     1501 tacttcccgc gcgcctccca gcggatggtg gcgaccgcgc cgctccgcgt ggccgagtcc
     1561 gagggtcggt tcgacgtccg tgtgcgcctg cacggcggcg gcccgtcggg ccaggccggc
     1621 gcgctgcgcc acggtctcgc ccgcgcgctc gtcgaggccg atcccgcgct gcggacggcg
     1681 ctcaagcgcg agggcttcct cacgcgtgac gcccgcatcg tggagcgcaa gaaggccggc
     1741 ctccacaagg cgcgcaaggc gccccagtac agcaagcgct gaatcgcttc tctcgtgggt
     1801 cccaggccgc ttcgggccgc gccgggccga gcccggcaag gcgacgaacg ccgggagctc
     1861 gacgacttag taacacagtg ttactaggtc tgggttccac ccgcgctcgg tcgctcgatt
     1921 agcgtttcgt ccatgggtga cgggcggcgg tacttcggga ccgacggggt gcggggggtc
     1981 gtgggcgact cgctcacgga ggatctcgtg gcgcgggtcg ggttcgcgtt cgcgcaatgg
     2041 agcgacgcgg cgcccgtgct cgtggggcgc gacacgcgcg cgtcggggcc ggcgctcgag
     2101 gcggcgctcg ctcgagggct cgccgccgcc ggttcggagg tcacgctcgg cggcgtcctc
     2161 ccaacgcccg ccgtcgcgct gctcgccgac ggctgcgggg cggtcgtctc cgccagccac
     2221 aatccgccgg agtacaacgg cgtcaagctc ttcgcgcgcg aggggcgcaa gctcacggac
     2281 gaggacgagg cgctcgtcga gggcctcctg ccggagcagg tgccgaccgt cgacgccgag
     2341 ccgcgccgcg acgacaccgc ccgcgaccgc tacctcgagc tcgtgaccga gaggttcggc
     2401 gcgccgctcg acgggctccg gatcgcggtc gacacggcga acggcgcgat gagcggcctc
     2461 gccgaggagg cgttcgagcg gctcggggca cgcgtcacgg cgctcgggaa cgcacccgac
     2521 gggacgaaca tcaacgtcgg ctgcggcgcg accgacccgg ccgcgctcgc cgccgggacg
     2581 cgcgcgagcg agcacgacct cggcgtcgcg ctcgacgggg acggcgaccg cgtggtcgcg
     2641 gtcgacgccg ccgggaacga ggtgaacggc gac
//
LOCUS       DASTFC010000347         9796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1148781,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000347 DASTFC010000000
VERSION     DASTFC010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148781"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1030
                     /locus_tag="VFO88_10250"
     CDS             50..1030
                     /locus_tag="VFO88_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9545944.1"
                     /translation="MADGSLLVTPDDVARASRRVAGRLHRTPMLTSRTLGEAFGGTAL
                     LKAELFQRTGSFKPRGLLARLAGLDADARAKGVVTVSAGNAAAALAYGAALEGVDCLV
                     CMWQGASPHKVAATQAYGATVDQEAAGPEEAFARVQGIAEETGRTFVHPFDDHDVIAG
                     HGSLGYELVEDAPDVDVVVVPVGGGGLVSGVTVAVKDARPDARVVAVEPEGSAALHAA
                     LLAGRPVKVVPTSIADGLNAPFAGRRCVAVTREHDVETVLVTEDEIRDGFRFLYGRAK
                     LAVEPAAAATAGALLAGRIGGLEGRTVVSVVSGGNVSARMASDILASHEA"
     gene            1020..1235
                     /gene="rpmE"
                     /locus_tag="VFO88_10255"
     CDS             1020..1235
                     /gene="rpmE"
                     /locus_tag="VFO88_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET9545945.1"
                     /translation="MKPDIHPEYVTATVTCSCGNTFETRSTKPELHVEICSECHPFYT
                     GKQKLMDTGGRVERFQRRLERAGQANR"
     gene            1297..2256
                     /locus_tag="VFO88_10260"
     CDS             1297..2256
                     /locus_tag="VFO88_10260"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET9545946.1"
                     /translation="MTALRRLRLLLPFASAAPIGGQAVLEGVMMRSPSAWSVAVRKPD
                     GEIAEVNQPISSFSLRHRWARIPVVRGVIALGESLAIGFRALAISANYAAQEENEDGE
                     DISTELTRGQLLFAFGLAIGFALLLFKVSPAIITSFLPVDGTFAFVVVEGLVRVGIFI
                     AYLLLISLLPDLKRVFQYHAAEHKAINALEAGDELTPERVQRYSLLHVRCGTAFLLYV
                     MVIAIFVFAFFGQPAWYWLIASRILLLPVIAGLAYEVIRFAGKHPEHRALRTLLAPGL
                     WLQRLTTREPTLDQVEVSIRALREVLRIEGRITPEERKVEVMA"
     gene            2284..3333
                     /gene="prfA"
                     /locus_tag="VFO88_10265"
     CDS             2284..3333
                     /gene="prfA"
                     /locus_tag="VFO88_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET9545947.1"
                     /translation="MAIEDLVAELERSHAEAQERMSDPAVFADHKTAADAGRRLKELE
                     GPYKLAQEWREASADLADARGDGELRELVPDLEERVARLEEELKLALVERDPADAKDV
                     IVEIRQGVGGDEAAIWAGDLVRMLTRYAERRGFRVELLGGSPSEAGGYKDATLAIKGD
                     GAYSVFKWEGGTHRVQRVPETESQGRIHTSTATVAVMPEAEEVEVEIDPNDLKVDVYR
                     STGPGGQSVNTTDSAVRITHVPTGVVVAMQDEKSQLQNKQKAMRVLRARLYELERRRQ
                     EEELSAQRRSQIGSGERSEKIRTYNFPESRVTDHRVKVTVHQLDRVLDGELAEFTEAL
                     ADEERRRALESAAIA"
     gene            3330..4199
                     /gene="prmC"
                     /locus_tag="VFO88_10270"
     CDS             3330..4199
                     /gene="prmC"
                     /locus_tag="VFO88_10270"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9545948.1"
                     /translation="MRPPQAVADVERLLAEAGVPDPRVDAEILVAEAHGVLRSALFVP
                     DGELTAAALARLEELVARRRRREPLQYVLGEWGFRRLTLAVDRRALIPRPETEVLVER
                     CLALVAEIAEPRILDVGVGSGAIALALADERPDARITAVDASAGALSLARENAERTGL
                     ADRVELVHGNLFAGVAGPFDLVVSNPPYVAESEIATLEPEVRDWEPREALVGEGVTEA
                     VAAGARAVLEPGGAVVLETAGGKAGEVAALLERLGYGDVLVTPDLTDVERIAEGRVTL
                     ARTQTPREGEPSK"
     gene            4196..5056
                     /locus_tag="VFO88_10275"
     CDS             4196..5056
                     /locus_tag="VFO88_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9545949.1"
                     /translation="MTRFTRLFALLLALAALGLAGCGGDDGDDDAATNGTETTETTEE
                     AAFETVNEGVLTVGSDIPFPPFEFREGDDLTGFDVELVEEIASRLGLESVEWTPTAFE
                     TIFTQLATGRFDMIASATTITDEREELINFTDPYYRAQQALIVNSEATPDIQSTADVG
                     EGHTIAVQRGTTGEAWARENLEPQGAEIRSFPQAPDTYIALESGNVQGVIFDEPSAVE
                     EAANREGLEVVEAIDTGEDYGLGVNPDNEALLDEVNRVLAEMIEDGGYQEIYDRWFPD
                     APAGSVAEGS"
     gene            5136..6245
                     /locus_tag="VFO88_10280"
     CDS             5136..6245
                     /locus_tag="VFO88_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET9545950.1"
                     /translation="MSEAQGAGPDPGQVAERRMPDRGLRYLLTEQTALSAGIGALGAT
                     ALATLLVGLAGVVTAYGWTPSENVADALTSVELGAVIWIAVALGAVSLALGLATARRM
                     PTRASREGAVAGAALGGQAVALALFLVWFRESADVDRFARNFFEFVFVTDFWERFVRG
                     ALNTVILAAGSEALGIAIGLVFVVFAISKRAVVRAPARIYINFFRGTPLLWQLFFFYF
                     GIVLGLQVDMTVYQAAILVLGLNAGAYSAEIFRAGVQSIERGQFEAARSLGMGYMQAL
                     RLVILPQAIRRVIPPLTNEFVILIKDTSLVSVLGLTFAQQELMSVGREIYASSGNATA
                     FLGSAAGYLAITLPLIWLVGRLEKKLRSGLTGIGA"
     gene            6245..7003
                     /locus_tag="VFO88_10285"
     CDS             6245..7003
                     /locus_tag="VFO88_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET9545951.1"
                     /translation="MSDRGLNDDGSLVKLEDIHKWFGPELYVLRGIDLAVVPGEVLVV
                     IGPSGSGKSTLLRTINMLEEPTEGRVVFEGRELTDVRANLNEARTHIGMVFQSFNLFP
                     HKKAVDNVSMALRKVLGMNAGDAKRRALEQLEAVGLADKANAYPGQLSGGQQQRVAIA
                     RALAMRPRLMLFDEVTSALDPELVQEVLDVVKALADEGMTMVVVTHEMGFAREVGSRL
                     IFMDDGQIVEEGDPRAVFENPTEERTQAFLSKVL"
     gene            7000..7614
                     /locus_tag="VFO88_10290"
     CDS             7000..7614
                     /locus_tag="VFO88_10290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9545952.1"
                     /translation="MSAVEDAVAGIKAGKPVVLPTDTVYGLCADAYREAPVLRLGKLK
                     GRPEGMPVALVAADLDMILDAVPAIRGRAAVACRALLPGPYTLVVPNPARRFRWIGGS
                     RPDTIGVRVPDLPAAAREVVERVGAVAATSANLHGGPDARRVDELPPELLDAVAAVVD
                     AGELPGTPSTVVDLTGPEPVVLREGAVPAAEALSALAASRPGQE"
     gene            complement(7577..7879)
                     /locus_tag="VFO88_10295"
     CDS             complement(7577..7879)
                     /locus_tag="VFO88_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545953.1"
                     /translation="MTLETAFPLAIVALAVFLPLGREYAAFRREWGVGRLAALGTAFS
                     LFPALGVGLALSQPLAATPAAQWAVIVVVTILAYSLAVAVLRPRLTPARVETRRER"
     gene            7987..9282
                     /gene="glyA"
                     /locus_tag="VFO88_10300"
     CDS             7987..9282
                     /gene="glyA"
                     /locus_tag="VFO88_10300"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9545954.1"
                     /translation="MAVAEETLQHLREARLDELDGDVAALLGRELDRQRSTIELIASE
                     NFTWPAVFEAQASVPTNKYAEGYPGRRYYGGCEVVDEIEQLAIDRAKELFGADHANVQ
                     PHAGAQTNMAVYFAALQPGDTILAMRLDHGGHLTHGLKVNFSGRLYDVATYGVDRDTG
                     LVDFDEVLRTAKECRPKLIVCGASAYPRAIEADRFREIADEIGALLLCDMAHIAGLVA
                     AGLHPNPVPHCDFVTSTTHKTLAGPRAGLVLCKAEHADAVDKAVFPGMQGGPLLHTIA
                     AKATCFKIAMSEGFRAYQRQIRANADALADTLLERGHELLTGGTDTHLVQVDLRRSDW
                     TGRDAEERLDEVGITANRNTVPFDERPPTVASGLRLGTPAVTMRGFLEDDLREVGEII
                     ASALAADGDLGALRARSEELCRRHPLYPGFRGYATYATG"
     gene            9279..>9796
                     /gene="upp"
                     /locus_tag="VFO88_10305"
     CDS             9279..>9796
                     /gene="upp"
                     /locus_tag="VFO88_10305"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF001097.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET9545955.1"
                     /translation="MSLLAAGTVVEVTHPLVRHKLGLLRDESTPTQLFRQLVDELTLL
                     LTYEATKDLATEDVVVRTPLEETTVERISGKKVAVCPILRAGIGMLDGVLSLISGARV
                     GFIGLYRDEESLEPVEYYVKLPQDIASRDVLLLDPMLATGHSTAYATRLVKEAGAASV
                     RLIALIAAPEGVR"
BASE COUNT         1402 a         3508 c         3550 g         1336 t
ORIGIN      
        1 ggcggctccg ggcccgagta ctcctcgatg taggcgtagg ctgcgcggca tggccgacgg
       61 ctcgctcctc gtcacgccgg acgacgtggc gcgcgcctcg cgccgggtcg cgggccggct
      121 ccaccgcacg ccgatgctca cctcgcgcac gctcggcgag gcgttcggcg ggacggcgct
      181 cctgaaggcg gagctcttcc agcgcacggg ctccttcaag ccgcgcggcc tcctggcccg
      241 gctcgcgggc ctcgacgctg acgcgcgcgc gaagggcgtc gtgacggtct cggcaggcaa
      301 tgcggcggcc gcgctcgcct acggcgccgc gctcgagggg gtggactgcc tcgtctgcat
      361 gtggcagggc gcgagcccgc acaaggtcgc cgcgacgcag gcgtacggcg cgacggtcga
      421 ccaggaggcg gcgggccccg aggaggcgtt cgcccgcgtg caggggatcg cggaggagac
      481 cggccgcacg ttcgtccacc ccttcgacga ccacgacgtg atcgcggggc acggctcgct
      541 cggctacgag ctcgtcgagg acgcgcccga cgtggacgtc gtcgtcgtcc cggtcggcgg
      601 cggcggcctc gtctccggcg tgacggtcgc ggtgaaggac gcgcggccgg acgcgcgcgt
      661 cgtcgccgtc gagccggagg gctccgccgc gctccacgcg gcgctcctcg ccgggcggcc
      721 ggtgaaggtc gtcccgacct cgatcgccga cggcttgaac gccccgtttg ccggccgccg
      781 ctgcgtggcg gtgacgcggg agcacgacgt cgagacggtg ctcgtgaccg aggacgagat
      841 ccgcgacggg ttccgcttcc tctacgggcg cgcgaagctc gccgtcgagc ccgccgcggc
      901 cgcgacggca ggcgcgctcc tggccgggcg aatcggcggt ctcgagggca ggacggtcgt
      961 ctccgtcgtc agcggcggca acgtctcggc ccgcatggcc tctgatatcc tggcctccca
     1021 tgaagcctga catccacccc gagtacgtga ccgcaacggt cacctgctcc tgcggcaaca
     1081 cgttcgagac gcggtcgacg aagccggagc tccacgtcga gatctgctcg gagtgccatc
     1141 ccttctacac cggcaagcag aagctcatgg acaccggcgg tcgggtcgag cgcttccagc
     1201 gccgtctcga gcgcgcgggc caggcgaacc gctagccgac cgccttgcag cacgcaacgc
     1261 tcaaggaaac cgcccggagc gtccgataac gagcccatga ccgcccttcg tcgccttcgc
     1321 ctgctgctcc ccttcgcctc cgccgccccg atcggcggcc aggcggtgct cgagggcgtg
     1381 atgatgcgca gcccgtccgc gtggtcggtc gccgtccgca agccggacgg ggagatcgcg
     1441 gaggtgaacc agccgatctc ctcgttctcg ctgcgccacc gctgggcgcg gatcccggtc
     1501 gtccgcggcg tgatcgcgct cggcgagtcg ctcgcgatcg gcttccgcgc gctcgccatc
     1561 tcggcgaact acgccgcgca ggaggagaac gaggacggcg aggacatctc gaccgagctc
     1621 acgcgcggcc agctcctctt cgcgttcggc ctcgcgatcg gcttcgcgct cctgctcttc
     1681 aaggtctcgc cggcgatcat cacgagcttc cttccggtcg acggcacgtt cgcgttcgtc
     1741 gtcgtcgagg ggctcgtccg cgtcggcatc ttcatcgcgt acctcctgct catctcgctc
     1801 ctgcctgacc tcaagcgggt cttccagtac cacgcggccg agcacaaggc gatcaacgcg
     1861 ctcgaggccg gcgacgagct gacgcccgag cgggtgcagc gctactcgct cctccacgtc
     1921 cgctgcggga cggcgttcct gctctacgtg atggtgatcg cgatcttcgt cttcgcgttc
     1981 ttcggccagc cggcctggta ctggctgatc gcgtcgcgga tcctgctcct gccggtcatc
     2041 gcgggcctcg cgtacgaggt gatccggttc gccgggaagc atcccgagca ccgcgcgctc
     2101 cgcacgctcc tcgcgcccgg cctgtggctc cagcgcctga cgacgcgcga gccgaccctc
     2161 gaccaggtcg aggtctcgat ccgagcgctc cgggaggtcc tccgcatcga gggccgcatc
     2221 accccggagg agcgcaaggt cgaagtcatg gcataagcag cccctcgggc tacgcttgac
     2281 gccatggcca tcgaagacct cgtcgcggag ctcgagcgct cgcatgcgga ggcgcaggag
     2341 cgcatgtccg accctgccgt cttcgccgac cacaagaccg cggcggacgc gggccgccgg
     2401 ctgaaggagc tcgaggggcc gtacaagctc gcgcaggagt ggcgcgaggc gagcgccgac
     2461 ctcgcggacg cccgcggcga cggggagctt cgcgagctcg tccccgacct cgaggagcgg
     2521 gtcgcgcggc tcgaggagga gctgaagctc gcgctcgtcg agcgcgaccc ggccgacgcg
     2581 aaggacgtga tcgtcgagat ccgccagggc gttggcggcg acgaggcggc gatctgggcc
     2641 ggcgacctcg tccgcatgct gacccgctac gccgagcggc gcggcttccg cgtcgagctg
     2701 ctcggcggga gcccgagcga ggcgggcggc tacaaggacg cgacgctcgc gatcaagggc
     2761 gacggcgcgt actcggtctt caagtgggag ggcgggacgc accgcgtcca gcgcgtcccg
     2821 gagacggagt cgcagggccg catccatacc tcgacggcca ccgtggccgt gatgcccgag
     2881 gcggaggagg tcgaggtcga gatcgacccg aacgacctca aggtcgacgt ctaccgctcg
     2941 accggtcccg gcggccagag cgtgaacacg accgactcgg cggtgcggat cacgcacgtc
     3001 ccgaccgggg tcgtcgtcgc gatgcaggac gagaagtcgc agctccagaa caagcagaag
     3061 gcgatgcgcg tcctgcgcgc ccggctctac gagctcgagc gccgccggca ggaggaggag
     3121 ctctccgcgc agcgccgctc gcagatcggc tcgggcgagc gctcggagaa gatccgcacg
     3181 tacaacttcc cggagagccg tgtgaccgac caccgcgtga aggtcaccgt ccaccagctc
     3241 gaccgggtgc tcgacggcga gctcgcagag ttcacggagg cgctcgccga cgaggagcgc
     3301 cgccgcgcgc tcgagtccgc ggcgatcgcg tgaggccccc gcaggccgtc gccgacgtcg
     3361 agcggttgct cgccgaggcc ggcgtgccgg acccgcgcgt cgacgcggag atcctcgtcg
     3421 cggaggcgca cggcgtcctg cgctcggcgc tcttcgtccc ggacggggag ctcaccgccg
     3481 ccgcgctcgc gcggctcgag gagctcgtcg cccgacggcg gcgtcgcgag ccgctccagt
     3541 acgtcctcgg cgaatggggc ttccggcggc tgacgctcgc cgtcgaccgc cgcgcgctca
     3601 tcccgcgccc cgagacggag gtgctcgtcg agcgctgcct cgcgctcgtc gcggagatcg
     3661 ccgagccgcg gatcctcgac gtcggcgtcg gctccggcgc gatcgcgctc gcgctcgccg
     3721 acgagcgtcc ggacgcacgc atcacggcgg tcgacgcctc ggccggcgcg ctctcgctcg
     3781 cgcgggagaa cgcggagcgg acgggcctcg ccgaccgcgt cgagctcgtc cacggcaacc
     3841 tcttcgccgg cgtcgcgggc ccgttcgacc tcgtggtatc gaacccgccc tacgtggccg
     3901 agtcggagat cgcgacgctc gagccggagg tgcgcgactg ggagccgcgc gaggcgctcg
     3961 tgggcgaggg agtcaccgag gcggtcgccg cgggcgcgcg cgccgtcctc gagcccggcg
     4021 gggcggtcgt gctggagacg gcgggcggaa aagcaggaga ggtcgcggcc ctcctcgagc
     4081 gcctcggcta cggcgacgtc ctggtcacgc ccgacctaac cgacgtggag cggatcgccg
     4141 agggtagggt gacgttggcg aggacgcaaa ctccgaggga gggagaaccg tccaaatgac
     4201 acgcttcaca agactgttcg cgcttctcct cgcgctcgcc gcgctcggcc tcgccgggtg
     4261 cggcggagac gacggtgacg acgacgcggc gacgaacgga acggagacca cggagacgac
     4321 cgaggaagcg gccttcgaga cggtgaacga gggcgtcctg accgtcggat cggacatccc
     4381 gtttccgccc ttcgagttcc gcgagggcga cgacctgacc ggcttcgacg tcgagctcgt
     4441 cgaggagatc gcctcgcgcc tcggcctcga gagcgtcgag tggaccccga ccgcgttcga
     4501 gacgatcttc acgcagctcg cgaccggccg cttcgacatg atcgcgtcgg cgacgacgat
     4561 cacggacgag cgcgaggagc tcatcaactt caccgacccg tactaccgcg cccagcaggc
     4621 cctgatcgtg aacagcgagg cgacgcccga catccagtcg accgccgacg tcggcgaagg
     4681 ccacacgatc gcggtccagc gcggcacgac cggcgaggcc tgggcgcgcg agaacctcga
     4741 gccgcagggc gccgagatcc gctcgttccc acaggcgccg gacacctaca tcgccctcga
     4801 gtcggggaac gtccagggcg tcatcttcga cgagccgtcc gcggtcgagg aggctgcgaa
     4861 ccgcgagggg ctcgaggtcg tcgaggcgat cgacaccggc gaggactacg ggctcggcgt
     4921 caatccggac aacgaggcgc tgctcgacga ggtgaaccgg gttctcgccg agatgatcga
     4981 ggacgggggc taccaggaga tctacgaccg ctggttcccg gacgcgcccg cggggagcgt
     5041 cgccgaaggc tcctgaacgg gcttaacctt cacgcctgac caacagagag gggcggcccg
     5101 gcagccgccc ctctcacgca ggcgaggagg cggagatgag cgaagcacaa ggcgcagggc
     5161 ccgacccggg gcaggtggcc gaacggcgga tgccggaccg aggcctccgc tatctcctca
     5221 cggagcagac ggccctctcg gcagggatcg gggcgctggg cgcgaccgcg ctcgcgaccc
     5281 tgctcgtcgg tctcgcgggg gtcgtcacgg cgtacgggtg gactccgtcc gagaacgtcg
     5341 cggacgcgct cacgagcgtc gagctcgggg cggtcatctg gatcgccgtg gcgctcggcg
     5401 cggtctcgct cgcgctcggg ctcgcgaccg cgcggcggat gccgacccgg gcgagccggg
     5461 agggagccgt cgcaggggcg gccctgggcg gccaggcggt cgcgctcgcg ctcttcctcg
     5521 tctggttccg ggagagcgcc gacgtcgacc gcttcgcgcg gaacttcttc gagttcgtct
     5581 tcgtgaccga tttctgggag cggttcgtcc gcggcgcctt gaacacggtc atcctcgccg
     5641 ccggcagcga ggccctcggg atcgcgatcg ggctggtgtt cgtcgtcttc gcgatctcga
     5701 agcgggcggt cgtccgcgcc ccggcccgga tctacatcaa cttcttccgg gggacgccgc
     5761 tcctctggca gctcttcttc ttctacttcg ggatcgtcct cggcctccag gtcgacatga
     5821 cggtctacca ggccgcgatc ctcgtcctcg gcctgaacgc gggcgcctac tcggcggaga
     5881 tcttccgggc cggcgtgcag tcgatcgagc gagggcagtt cgaggccgcc cggtcgctcg
     5941 ggatggggta catgcaggcg ctccgcctcg tgatcctccc gcaggcgatc cgccgtgtga
     6001 tccccccgct cacgaacgag ttcgtgatcc tgatcaagga cacgtcgctc gtctccgtgc
     6061 tcggcctgac gttcgcccag caggagctga tgtcggtggg gcgagagatc tacgcgagca
     6121 gcggcaacgc gaccgcgttc ctcggctcgg ctgcgggcta cctcgccatc acgctgccgc
     6181 tcatctggct cgtggggcgc ctggagaaga agctgcgcag cggcctgacc gggatcggtg
     6241 cctgatgagc gaccgcggac tcaacgacga cggatcgctc gtcaagctcg aggacatcca
     6301 caagtggttc ggcccggagc tctacgtcct gcgtggcatc gatctcgccg tggtcccggg
     6361 ggaggtgctc gtcgtcatcg ggccgtccgg ctccggcaag tcgacgctcc tgcgcacgat
     6421 caacatgctc gaggagccga ccgagggccg cgtcgtcttc gaggggcggg agctcaccga
     6481 cgtgcgggcc aacctgaacg aggcgcggac gcacatcgga atggtcttcc agagcttcaa
     6541 cctcttcccg cacaagaagg cggtcgacaa cgtctcgatg gcgctccgca aggtgctcgg
     6601 gatgaacgcg ggggacgcga agaggcgggc gctcgagcag ctcgaggcgg tcgggctcgc
     6661 ggacaaggcg aacgcgtacc ccggccagct ctccggcggc cagcagcagc gggtcgcgat
     6721 cgcgcgcgct ctcgcgatgc gcccacggct catgctcttc gacgaggtga cgtcggcgct
     6781 cgaccccgag ctcgtgcagg aggtgctcga cgtggtcaag gcgctcgccg acgaggggat
     6841 gacgatggtc gtcgtcacgc acgagatggg cttcgcgcgc gaggtcgggt cgcggctcat
     6901 cttcatggac gacgggcaga tcgtcgagga gggcgacccg cgcgccgtgt tcgagaaccc
     6961 gacggaggag cggacgcagg cgttcctctc caaggtgctg tgagcgcggt cgaggacgcg
     7021 gtcgcgggga tcaaggccgg gaagccggtc gtcctcccga ccgacaccgt gtacgggctc
     7081 tgcgccgacg cctaccgcga ggcgccggtc ctccggctcg ggaagctcaa ggggcggccg
     7141 gaggggatgc ccgtcgcgct cgtcgccgcg gacctcgaca tgatcctcga cgcggtgccg
     7201 gcgatccgcg gccgcgcggc cgtcgcgtgc cgcgcgctgc tcccggggcc gtacaccctc
     7261 gtcgtcccga accccgcccg gcggtttcgc tggatcggcg gctcgcggcc ggacacgatc
     7321 ggggtgcggg tccccgatct cccggcggcc gcgcgcgagg tcgtcgagcg cgtcggggcg
     7381 gtcgcggcga cgagcgcgaa cctccacggc gggcccgacg cgcggcgggt cgacgagctg
     7441 ccgcccgagc tcctcgacgc ggtcgccgcg gtcgtggacg ccggcgagct gcccggcacg
     7501 ccgtccacgg tggtcgacct caccgggccc gagccggtcg tgctgcgcga gggcgccgtg
     7561 cccgccgccg aggcgctcag cgctctcgcc gcgtctcgac ccgggcagga gtgagccgcg
     7621 ggcgcaggac ggcgacggcg agcgagtagg cgaggatcgt caccacgacg atcaccgccc
     7681 actgcgcggc gggcgtcgcg gcgagcggct gcgagagggc gaggccgacg ccgagcgccg
     7741 ggaagagcga gaacgccgtc ccgagcgcgg cgagccgccc gacgccccac tcgcgccgga
     7801 acgccgcgta ctcgcgcccg agcggcagga acaccgcgag ggcgacgatc gcgagcggga
     7861 atgcggtttc gagtgtcatg gcggcctcga ttacctgaac gccgcatccc ctaaccgggt
     7921 tacgccgaat atcactcgcc cgagggatgc gactccctcg ttcgaggcac gcgctaggct
     7981 ccggacgtgg cggtcgcaga ggagacgttg cagcacctcc gcgaggcgcg gctcgacgag
     8041 ctggacggcg acgtcgccgc gctcctcggg cgggagctcg accgccagcg ctccacgatc
     8101 gagctgatcg cgtcggagaa cttcacgtgg ccggccgtgt tcgaggcgca ggcgagcgtc
     8161 ccgacgaaca agtacgcgga gggctatccg ggccggcgct actacggcgg ctgcgaggtc
     8221 gtggacgaga tcgagcagct cgcgatcgac cgggcgaagg agctcttcgg cgccgaccac
     8281 gcgaacgtcc agccgcacgc gggcgcgcag accaacatgg ccgtctactt cgccgcgctc
     8341 cagccgggcg acacgatcct cgccatgcgg ctcgaccacg gcgggcacct cacgcacggg
     8401 ctcaaggtga acttctccgg gcggctctac gacgtggcga cgtacggcgt cgaccgcgac
     8461 accggcctcg tcgacttcga cgaggtcctc cgcaccgcca aggagtgccg cccgaagctc
     8521 atcgtctgcg gcgcctccgc gtacccgcgg gcgatcgagg ccgaccggtt ccgcgagatc
     8581 gccgacgaga tcggggccct gctcctctgc gacatggcgc acatcgccgg gctcgtcgcg
     8641 gccgggctgc atccgaatcc cgtgccgcac tgcgacttcg tcacctcgac gacgcacaag
     8701 acgctggcgg ggccgcgtgc cgggctcgtg ctctgcaagg cggagcacgc cgatgcggtc
     8761 gacaaggcgg tcttcccggg gatgcagggc ggcccgctcc tgcacacgat cgcggcgaag
     8821 gcgacctgct tcaagatcgc catgtcggag gggtttcgcg cgtaccagcg ccagatccgc
     8881 gcgaacgcgg acgcgctcgc cgacacgctt ctcgagcgcg ggcacgagct cctgaccggc
     8941 gggacggaca cgcacctcgt ccaggtcgac ctccgccgga gcgactggac gggccgagac
     9001 gcggaggagc ggctcgacga ggtcgggatc accgcgaacc ggaacaccgt gccgttcgac
     9061 gagcggccgc cgaccgtcgc ctcgggcctg cggctcggga cgccggcggt gacgatgcgc
     9121 gggttcctcg aggacgacct gcgcgaggtg ggggagatca tcgcctcggc gctcgcggcg
     9181 gacggcgacc tcggcgcgct ccgcgcccgc agcgaggagc tctgccggcg gcatccgctc
     9241 tacccgggct tccgcggcta cgcgacgtac gcgaccggat gagcctcctc gcggccggca
     9301 ccgtcgtcga ggtcacgcac ccgctcgtcc ggcacaagct cgggctgctc cgggacgagt
     9361 cgacgccgac gcagctcttc cgccagctcg tcgacgagct cacgctcctc ctcacgtacg
     9421 aggcgacgaa ggacctggcg acggaggacg tggtcgtccg cacgccgctc gaggagacga
     9481 ccgtcgagcg catctccggc aagaaggtcg ccgtctgccc gatcctccgg gccgggatcg
     9541 ggatgctcga cggcgtcctc tcgctcatct cgggcgcgcg cgtcggcttc atcgggctct
     9601 accgcgacga ggagtcgctc gagccggtcg agtactacgt gaagctcccg caggacatcg
     9661 cgtcgcgcga cgtgctcctc ctcgacccga tgctcgcgac gggccactcg acggcgtacg
     9721 cgacgcggct cgtgaaggag gcgggcgcgg cgagcgtgcg gctgatcgcg ctcatcgcgg
     9781 cgccggaggg cgtccg
//
LOCUS       DASTFC010000348         6260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_373868,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000348 DASTFC010000000
VERSION     DASTFC010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373868"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..548)
                     /locus_tag="VFO88_10310"
     CDS             complement(63..548)
                     /locus_tag="VFO88_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9545956.1"
                     /translation="MAVVRWEPFRELAALQSEMSRWMNQVAGTGSAGPGNGTSTWLPA
                     VDVWESDSELVLSFDLPGIEEDRISVELEDNVLTVSGERDRAEKTEGDRFYRYERRFG
                     QFSRSVTLPPGVNEDEIKADYKAGVLEVRVPKPEEQKPKRIPVSGSEQTAIEGKGTRT
                     S"
     gene            672..1610
                     /gene="argF"
                     /locus_tag="VFO88_10315"
     CDS             672..1610
                     /gene="argF"
                     /locus_tag="VFO88_10315"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET9545957.1"
                     /translation="MIPVHLKGRSYLRVNDWSPEELTTVLDLADRLKARHKSGVADRV
                     LEGRTLGMIFTKPSTRTRVSFETGIWQLGGTGLYLSANDLQLGRGETIRDTAVVLSRY
                     LDGIMIRTFDQSDVDGLAEHAEIPVINGLTDSFHPCQALADVMTVRERFGKLEGVRVA
                     YLGDGNNVCHSLMVACAKLGASFVAATPEGYEPEDEVVGWARAAAEASGGEVGLTHDR
                     DEAIRSANVLYTDVWTSMGQDEERERRLRDLEPYRIDDRVLRLASGNAIVMHCLPAHY
                     GEEVTEAVLHGSQSAAWDQAENRLHAQKALMALVIA"
     gene            1625..2320
                     /locus_tag="VFO88_10320"
     CDS             1625..2320
                     /locus_tag="VFO88_10320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9545958.1"
                     /translation="MLPLKDTIPTRRFPVLTVAFIAANVAVWLLVQDAGAEPGFSQSL
                     VDLAYRPCEVDGACADRGESWPVNAFAAMFMHGDWLHLGGNMLFLWIFGNNIEDELGR
                     VRFVVFYLAGGLAATALQTFVVLNWGSADDATLPNVGASGAIAAVLGGYILLHPRSLI
                     LTWIAPIFFVPIPAFLYLGIWFVLQIVSGGSLLTSPETGGGVAYFAHIGGFVFGLLTV
                     KLFHAGRSQEHAW"
     gene            2317..2658
                     /locus_tag="VFO88_10325"
     CDS             2317..2658
                     /locus_tag="VFO88_10325"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET9545959.1"
                     /translation="MNAAEFEEHVVRALDGLPPDLRKAMSNVDIVVEEANAEEPDLYG
                     LYYGIALTERDSGYAGVLPDKISIYRRPLVEDFGHDPELLEEEIQVTVVHEIAHHFGI
                     SDERLDELGWA"
     gene            complement(2676..3167)
                     /locus_tag="VFO88_10330"
     CDS             complement(2676..3167)
                     /locus_tag="VFO88_10330"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9545960.1"
                     /translation="MTEPAAPMPPAPPPAGGYMPPSGPSGPRANFGQRLVAVIVDAII
                     VTAVTWILLLVLAGIGDEGGAIVGYLLAIVFGVAYFAYLEGGPTGQTLGKKVMSIRVI
                     DANTGGPIGYGRGVGRYFARWISSIPCLLGYFWMLWDREKQTWHDKICSCVVVPTDAY
                     PVQ"
     gene            complement(3187..3570)
                     /locus_tag="VFO88_10335"
     CDS             complement(3187..3570)
                     /locus_tag="VFO88_10335"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HET9545961.1"
                     /translation="MTAAGATAAALRAPLEADGRDLRIAGGLMLGAGLLLPLLPEGAG
                     VACPLRTLTGVPCPLCGMTTSVTETLRLDLAEALAANPAGVAAVVAAVAVLAVRPARV
                     RVSASLAYVTLAAMWLFELHRFGFV"
     gene            complement(3567..4310)
                     /locus_tag="VFO88_10340"
     CDS             complement(3567..4310)
                     /locus_tag="VFO88_10340"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9545962.1"
                     /translation="MDFEDRIVIATPEGIDLELTLAGAGSRFAAALVDSLLQLALSLA
                     LWAVLVLGIGGYGVALFAVAAFLVGFGYHILFEVLASGRTPGKRWTGLRVVRAGGHPV
                     TFLPSATRNILRLVDWLPAVYIVGCISVLVTKRNQRLGDLAAGTLVIRDQARVPVPAP
                     PRLLYGQATPPAGLDDLPAWDVSGITAEELVAVRRFLERRHELELHARTHLAGQLHDR
                     LRPKTAGVPAEVTGERFLELVARAKAARV"
     gene            4314..5288
                     /locus_tag="VFO88_10345"
     CDS             4314..5288
                     /locus_tag="VFO88_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET9545963.1"
                     /translation="MNLDRFQREREPAWNELAGLVHAAGRRPERLGPERVRRLGALYR
                     SAAADLALARRRFRGDPVERRLETLVGQARHLVYDAPTRRIAPLRFFARDYWRLVASR
                     PVPLVVSAVLLLGPATLALVWALGDPGAAGGLVPGDYRSVTEPRPGGSDLGLTVEEEA
                     AFASQVFTNNIQVSFLAVAGGMALGLVTAFVLVFNGTLLGAVAGLATEAGNGRIFFEL
                     VTAHGVLELSCIVVAGAAGLRLGWSIVDPGRLRRVDSLAAEARRTVAIVLGTAPWFVL
                     AGLVEGFLTPAGTGLAAVLAIGFGLGALYWLLVVLLGRDRGGDPVALG"
     gene            5431..>6260
                     /locus_tag="VFO88_10350"
     CDS             5431..>6260
                     /locus_tag="VFO88_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET9545964.1"
                     /translation="MLEVWESGDPVGELCAYLDERAFFAGGAADAVADVFLAYGASDG
                     LRRVPLAAPPEPCPLPLAACRIRPSAEPVAPPGPFAIGDWETTWAPEDYAAAVEDVRS
                     AIERGDVYQVNLVQHLAASFRGDPAGLAARLAALRPLHPEPLAGDGWTIVSGSPEVFL
                     RRRGRTLTTFPIKGTRPLGDGDELAESEKDAAEHVMIVDLERNDLSRVCEPGSVRWPE
                     LMVERELAGVAHLVSRVEGLVREDASLADMLRATFPGGSVTGAPKIAALDLIAALEPV
                     G"
BASE COUNT          837 a         2249 c         2277 g          897 t
ORIGIN      
        1 cggttaacgc cgaggggcga ggccgaacgc ctcgcccctt cggaggagag atcgggacgg
       61 cgttacgagg tccgggttcc cttgccctcg atcgccgtct gctcggatcc gctgaccggg
      121 atccgcttgg gcttctgctc ctccggcttc gggacgcgca cctcgaggac gccggccttg
      181 tagtcggcct tgatctcgtc ctcgttcacg cccggcggga gcgtcacgct gcgcgagaac
      241 tggccgaagc gccgctcgta gcggtagaag cggtcaccct cggtcttctc ggcgcggtca
      301 cgctcgccgc tcacggtgag gacgttgtcc tcgagctcga cggagatccg gtcctcctcg
      361 atgccgggga ggtcgaacga gagcacgagc tccgagtcgg actcccagac gtccacggcc
      421 ggaagccacg tcgacgtccc gttgcctggg ccggcgctgc cggttcccgc aacctggttc
      481 atccagcggc tcatctcgct ctgaagcgca gcgagctcgc ggaagggctc ccaacgcacg
      541 actgccatcg aaaacctccc tttcgccgaa gttcgtttcc gcgtgacact ctaccaatga
      601 tctgcctgcc cggcaaggag aaaatctaag cgtcttgttg tcaatacatc ccggccgtag
      661 aatgcggcgc cgtgatcccg gttcacctca agggccgcag ctacctgcgc gtgaacgact
      721 ggagcccgga ggagctcacg accgtcctcg atctcgcgga ccggctcaag gcgcgccaca
      781 agagcggcgt cgccgaccgc gtgctcgagg ggcggacgct cgggatgatc ttcacgaagc
      841 cgtccacccg aacgcgggtc agcttcgaga cggggatctg gcagctcggc ggcacgggtc
      901 tctacctttc ggcgaacgac ctccagctcg ggcgcggcga aacgatcagg gacacggcgg
      961 tcgtcctctc gcgctacctc gacgggatca tgatccggac gttcgaccag tccgacgtgg
     1021 acgggctcgc cgagcacgcg gagatcccgg tcatcaacgg gctgacggac agcttccacc
     1081 cgtgccaggc gctcgccgac gtcatgacgg tccgcgagcg cttcgggaag ctcgagggcg
     1141 tgcgcgtcgc gtacctcggc gacggcaaca acgtctgcca ctcgctcatg gtcgcgtgcg
     1201 cgaagctcgg cgcctccttc gtcgccgcga cgcccgaggg ctacgagccc gaggacgagg
     1261 tcgtcggctg ggcgcgcgcg gcggccgagg cgagcggcgg cgaggtcggg ctcacgcacg
     1321 accgcgacga ggcgatccgc agcgcgaacg tcctctacac cgacgtctgg acgagcatgg
     1381 gccaggacga ggagcgcgag cgccggctgc gcgacctcga gccgtaccgg atcgacgacc
     1441 gtgtgctccg gctcgcgtcg gggaacgcga tcgtcatgca ctgcctcccg gcgcactacg
     1501 gcgaggaggt caccgaggcc gtcctgcacg gctcgcagtc ggccgcctgg gaccaggccg
     1561 agaaccgcct ccacgcgcag aaggcgctca tggcgctcgt catcgcctag cgggccggcg
     1621 gcccttgctc ccgctcaagg acacgatccc gacccggcgc ttcccggtcc tcaccgtcgc
     1681 gttcatcgcg gcgaacgtcg cggtctggct cctcgtccag gacgccggcg ccgagcccgg
     1741 attctcgcag tcgctcgtcg acctggccta ccggccctgc gaggtcgacg gcgcctgcgc
     1801 cgaccgcggc gagtcgtggc ccgtgaacgc gttcgccgcg atgttcatgc acggcgactg
     1861 gctccatctg ggcgggaaca tgctcttcct ctggatcttc ggcaacaaca tcgaggacga
     1921 gctcggccgc gtccggttcg tcgtcttcta cctcgccggc ggcctcgcgg cgacggcgct
     1981 ccagacgttc gtcgtcctca actggggctc cgcggacgac gcgacgctcc cgaacgtcgg
     2041 cgcgagcggc gcgatcgccg ccgtcctcgg cggctacatc ctcctccacc cgcggagcct
     2101 catcctgacc tggatcgcgc cgatcttctt cgtcccgatc ccggccttcc tctacctcgg
     2161 gatctggttc gtgctccaga tcgtctccgg ggggtccctg ctcacctcgc cggagaccgg
     2221 cggcggcgtc gcgtacttcg cccacatcgg cggcttcgtc ttcgggctcc tgaccgtgaa
     2281 gctcttccac gcgggccgct cgcaggagca cgcctggtga acgcggccga gttcgaggag
     2341 cacgtcgtcc gggcgctcga cggcctcccg cccgatctgc gcaaggcgat gtcgaacgtc
     2401 gacatcgtgg tcgaggaggc gaacgccgag gagcccgatc tctacgggct ctactacggg
     2461 atcgcgctga ccgagcgcga ctccggctac gcgggcgtcc tgccggacaa gatctcgatc
     2521 taccggcggc cgctcgtgga ggacttcggg cacgaccccg agctcctcga ggaggagata
     2581 caggtcaccg tcgtgcacga gatcgcgcac cacttcggga tctcggacga gcgcctcgac
     2641 gagctcggct gggcgtaggc cggcgccggg cgccgttact gcacgggata cgcgtcggtc
     2701 ggcacgacga cgcagctgca gatcttgtcg tgccacgtct gcttctcgcg gtcccagagc
     2761 atccagaagt acccgaggag acacgggatc gaggagatcc agcgtgcgaa gtaccggccg
     2821 acgccgcggc cgtagccgat cggtccgccg gtgttcgcgt cgatcacacg gatgctcatg
     2881 accttcttcc cgagcgtctg gccggtcggg ccgccctcga ggtacgcgaa gtacgcgacg
     2941 ccgaagacga tcgccaggag gtatcccacg atcgccccgc cctcgtcgcc gattcccgcg
     3001 aggacgagaa gcaggatcca ggtgaccgcg gtgacgatga tcgcgtccac gatcaccgcg
     3061 acgaggcgct gcccgaagtt cgcccgtggc ccggaggggc cggacggcgg catgtagccc
     3121 ccggcgggcg gcggcgcggg cggcatcgga gcggcgggct cggtcatctc tcctcctggc
     3181 ctgcggtcag acgaacccga agcgatggag ctcgaagagc cacatcgctg cgagcgtcac
     3241 gtacgcgagc gaggcggaga ctcgcacccg agccggacgg accgcgagga cggcgaccgc
     3301 cgccacgacc gccgcgacgc cggccgggtt cgcggcgagc gcctccgcga ggtcgagccg
     3361 gagcgtctcg gtcacgctcg tcgtcatccc gcagagcggg caggggacgc cggtgagcgt
     3421 ccggagcggg cacgccaccc cggctccctc cggaaggagg ggcaggagga ggcccgcgcc
     3481 gagcatgagc ccgccggcga tgcggaggtc gcggccgtcc gcctcgagcg gcgcgcgcag
     3541 ggcggcggcc gtcgcgccgg ccgcggtcac acccgagccg ccttcgcgcg cgcgacgagc
     3601 tcgaggaacc gctcgccggt cacctccgcc gggacgcccg cggtcttcgg ccgcagccgg
     3661 tcgtgcagct ggcccgcgag gtgcgtccgc gcgtggagct cgagctcgtg gcgccgctcg
     3721 aggaagcggc ggacggcgac gagctcctcg gcggtgatgc cgctcacgtc ccaggccggg
     3781 agatcgtcca ggcccgccgg cggggtcgcc tgcccgtaga ggagccgcgg cggcgccggc
     3841 accggcactc gcgcctggtc gcggatgacg agcgtcccgg ccgcgaggtc gccgaggcgc
     3901 tggttccgct tcgtcacgag gacgctgatg cacccgacga tgtagacggc cgggagccag
     3961 tcgaccaggc ggaggatgtt ccgcgtcgcg ctcggcaaga acgtcacagg gtggccgccg
     4021 gcgcggacga cgcgcaggcc ggtccagcgc ttccccggcg tgcggcccga ggcgagcacc
     4081 tcgaacagga tgtggtagcc gaacccgacg aggaaggccg cgacggcgaa gagcgcgacg
     4141 ccgtagccgc cgatcccgag gacgaggacc gcccagagcg cgagcgacag ggccagctgg
     4201 aggagcgagt cgacgagcgc ggcggcgaac cgtgatcccg cgcccgcgag cgtaagctcg
     4261 agatcgatgc cctccggcgt cgcgatcacg atgcggtcct cgaagtccac gagatgaact
     4321 tagaccgttt tcagcgggag cgggaaccgg cgtggaacga gctcgcgggc ctcgtccacg
     4381 cggccggccg ccgcccggaa cgactcgggc cggagcgcgt ccgccggctc ggcgcgctgt
     4441 accgctcggc cgccgccgac ctggcgctcg cgcggcggcg gttccgcgga gacccggtcg
     4501 agcggcggct cgagacgctc gtcgggcagg cgcggcacct cgtctacgac gccccgacgc
     4561 ggcggatcgc gccgctccgg ttcttcgccc gcgactactg gcggctcgtc gcgtcgcgtc
     4621 cggtcccgct cgtcgtctcg gccgtgctgc tcctcgggcc ggcgacgctc gcgctcgtct
     4681 gggcgctcgg ggatcccggc gcggcgggcg ggctcgtccc cggggactac cggtcggtga
     4741 cggagccgcg gccgggcggc tcggacctcg gcctcacggt cgaggaggag gccgcgttcg
     4801 cgagccaggt gttcacgaac aacatccagg tctcgttcct cgccgtcgcg ggcgggatgg
     4861 cgctcgggct cgtgacggcg ttcgtgctcg tcttcaacgg gacgctcctc ggcgcggtgg
     4921 ccgggctcgc gaccgaggcg gggaacgggc ggatcttctt cgagctcgtg accgcgcacg
     4981 gggtcctcga gctctcgtgc atcgtcgtcg ccggggcggc ggggctgcgg ctcgggtggt
     5041 cgatcgtcga ccctgggcgc ctccgccgcg tggactcgct cgcggccgag gcgcgccgca
     5101 cggtcgcgat cgtcctcggc acggcgccgt ggttcgtcct ggcggggctc gtggaggggt
     5161 tcctgacgcc ggcggggacg gggctcgcgg ccgtgctcgc gatcgggttc gggctcggcg
     5221 cgctctactg gctgctcgtc gttctgctcg gccgggatcg cggcggcgat ccggtcgcac
     5281 tcggctgagc ttcagtcgtc gggccggctc gggacgaacg gtgaccacgg ctgtgcctcg
     5341 tccacacggc gatcgtggac acggccggtg ccaggcaccg gtcgtgtccg ccggggacac
     5401 ggccgcgcgc cagggcgcta gcgtagggcc gtgctcgagg tctgggagag cggcgatccg
     5461 gtcggcgagc tgtgcgcgta tctcgacgag cgcgcgttct tcgccggcgg tgcggccgac
     5521 gcggtcgcgg acgtcttcct tgcgtacggg gcgtcggacg ggctgcggcg ggttccgctc
     5581 gcggcgccgc ccgagccgtg cccgttgccg ctcgccgcgt gccgaatccg cccctcggca
     5641 gagcccgtcg cgcccccggg cccgttcgcg atcggtgact gggagacgac ctgggcgccg
     5701 gaggactacg ccgcggcggt cgaggacgtc cgctccgcga tcgagcgcgg ggacgtgtac
     5761 caggtcaacc tcgtccagca tctcgcggcg tcgttccggg gcgacccggc cgggctcgcc
     5821 gcacggctcg cagcgctccg gccgctccat ccggagccgc tcgcgggcga cggctggacg
     5881 atcgtctccg gctcgcccga ggtcttcctg cggcgccgcg gccggacgct caccaccttc
     5941 ccgatcaagg gcacgcgccc gctcggcgac ggcgacgagc tcgcggagtc ggagaaggac
     6001 gcggccgagc acgtgatgat cgtcgatctc gagcgcaacg acctctcgcg cgtctgcgag
     6061 ccggggagcg tccgctggcc ggagctcatg gtcgagcgcg agctggccgg cgtggcgcac
     6121 ctcgtctcgc gggtcgaggg gctggtgcgc gaggacgcgt cgctcgccga catgctgcgg
     6181 gcgacgttcc cgggcggctc cgtgacgggc gcgccgaaga tcgcggcgct cgacctgatc
     6241 gcggcgctcg agccggtcgg
//
LOCUS       DASTFC010000349         4601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_258829,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000349 DASTFC010000000
VERSION     DASTFC010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258829"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFO88_10355"
     CDS             complement(<1..407)
                     /locus_tag="VFO88_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545965.1"
                     /translation="MAVLVVAAVALPLAYLVVRVAGAGADALDVLGRARLGSLILDTA
                     LLAVAVTGGAIAIGVPLAWLVTRSDLPGRRVWGVLVALPLVVPSYVAALTLLAAFGPR
                     GLLQRALEGPFGVERVPEVYGFPGAFGALVLST"
     gene            complement(458..1459)
                     /locus_tag="VFO88_10360"
     CDS             complement(458..1459)
                     /locus_tag="VFO88_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HET9545966.1"
                     /translation="MPRIAAAALAAALLVPLLAACGGDDDALTVYSGRSEGLVAPLLE
                     QFSEDTGIEVAVRYGDSAELAATIAEEGDGSPADVFFAQDAGALGSVAEEGLLAELDP
                     ELLDAVSPRFRDPEGRWVGLSGRARVVAYNTEELAEADLPDSIWEYAEEEWSGRVGFA
                     PTNASFQAMVSAMRLTEGDDRTREWLEALAANDPRLYENNLMSLEAVASGEVDVALVN
                     HYYLYEVLAEQPGAPVANHFLESGDPGALVNVAGAGILAGTDRAADAERLLAYLLEEG
                     QPFFAQETAEYPLVAGVEPREELPPLASVRGPDIELGELGGQLASTLELLNDVGLTP"
     gene            complement(1527..2555)
                     /locus_tag="VFO88_10365"
     CDS             complement(1527..2555)
                     /locus_tag="VFO88_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9545967.1"
                     /translation="MSEPMIAVEAVTKRFGAVAAVDRASLTLARGEFVALLGPSGCGK
                     TTLLRLIAGFETPDEGSIRLGGRLAAGPGAWVPPERRQAAMVFQDYALFPHLTVAENV
                     GFGLPRARRGARVPEALELVGLGGLERRYPHSLSGGQQQRVALARALAPEPEVVLLDE
                     PWSNIDPLRRSEMRDELAEILRGAGVTVLLVTHDREEAFSVADRIALMHDGRIEQTGT
                     PEQVYYEPSSRWSAEFVGASNVLPGQVEDGLVRTLFGAFPAPAANGAATVDVVLRPEL
                     LELVPDEAGEGEVVAREFRGHDVFYRVRLADGTTVCSQRPSTEHVPLGARVAVRPHDG
                     RVAVIAPV"
     gene            2607..3302
                     /locus_tag="VFO88_10370"
     CDS             2607..3302
                     /locus_tag="VFO88_10370"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9545968.1"
                     /translation="MLRATRLAGVTALLVAPAPAAAGTAPLVCLDPGHASRPNLTTEP
                     IGPGSSIRKIKDGGGAAGEARVVLRIAKRARIVLENGGLRVAMTRTGPDFTLGRGGNV
                     DRAKFCNRRNAALMIRIHADGSTDPARHGAATLYPAWRRGWTDDVFRPSLRAARLLQR
                     ALVLRTGARDLGLFRRGDLTGFNWANVPVVLVETGFMTNPAERRRLTSFPYQWKIARA
                     LARGAERFLGRRA"
     gene            complement(3299..3745)
                     /locus_tag="VFO88_10375"
     CDS             complement(3299..3745)
                     /locus_tag="VFO88_10375"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9545969.1"
                     /translation="MSVHTNEHTIEIDRPAPHVFAYLAASERRQRWMSALKESEQVSE
                     GPVGLGTKFKDVFEDHGQRFEIDAEIIEWEPHEKLVIDLKASAFESKVIQLLAPGENG
                     GTRLTTRMETEYKSALARLMAGVVTGHAQKQLEEDLATLKEILETS"
     gene            complement(3742..4077)
                     /locus_tag="VFO88_10380"
     CDS             complement(3742..4077)
                     /locus_tag="VFO88_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545970.1"
                     /translation="MPTVKQKAALLAGAVAGAVAAVVLRGRRAAREAPRDVPDPRAAE
                     LREKLAQAREEAADESDFEAAGMGAETIVAEEEGDVEAARRRVHEQGRAAAEEMRRTE
                     DDEEAAENA"
     gene            4233..>4601
                     /gene="rpsF"
                     /locus_tag="VFO88_10385"
     CDS             4233..>4601
                     /gene="rpsF"
                     /locus_tag="VFO88_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9545971.1"
                     /translation="MMLMLDAELADERQNEILTRARELVERGGGTWVGQESWGRRKLA
                     YEIDKKTEAHYHLLHFDCEPETLDELTRVLKITDGAMRHMAVRRPAGRTTPAAAAAPS
                     ADTAPELEPVGTPETETTTES"
BASE COUNT          612 a         1736 c         1687 g          566 t
ORIGIN      
        1 tacgtggaga ggacgagcgc gccgaacgcg ccggggaaac cgtagacctc cgggacgcgc
       61 tcgacgccga acggcccctc gagcgcccgc tggagcaggc cgcgcggacc gaacgcggcg
      121 aggagcgtga gggccgcgac gtagctcggg acgacgagcg ggagcgcgac gagcactccc
      181 cacacgcgcc tcccgggcag gtcgctccgc gtcacgagcc aggcgagcgg gacgccgatc
      241 gcgatcgctc cgccggtcac ggccaccgcg aggagcgccg tgtcgaggat gagcgagcct
      301 agccgggcgc ggccgaggac gtccagcgcg tccgcgcccg cgccggccac gcggacgacg
      361 aggtaggcga gcgggagggc gaccgcggcg acgacgagga cggccacggc gacgagcggc
      421 cacggaggcc gccgcggccg ccctcccgcc gccgcgctca cggcgtgagg ccgacgtcgt
      481 tcaggagctc gagcgtggac gcgagctgcc cgccgagctc tccgagctcg atgtcgggcc
      541 cgcgcaccga cgcgagcggc ggcagctcct cgcgcggctc gacgccggcg acgagcgggt
      601 actcggccgt ctcctgcgcg aagaacggct ggccctcctc gagcaggtac gcgagcaggc
      661 gctcggcgtc cgccgcccgg tcggtcccgg cgaggatccc ggcgcccgcg acgttcacga
      721 gcgcgcccgg gtcgcccgac tcgaggaagt ggttcgcgac cggcgcgccc ggctgctcgg
      781 cgagcacctc gtagaggtag tagtggttca cgagcgcgac gtcgacctcg ccgctcgcga
      841 cggcctcgag cgacatgagg ttgttctcgt agaggcgcgg gtcgttcgcg gcgagcgcct
      901 cgagccactc gcgcgtccgg tcgtcgccct ccgtcagccg catcgccgag accatcgcct
      961 ggaaggacgc gttcgtcggc gcgaagccga cgcgtccgct ccactcctcc tcggcgtact
     1021 cccagatcga gtccgggagg tcggcctcgg cgagctcctc cgtgttgtac gcgaccacgc
     1081 gcgcccggcc cgagaggccg acccagcgcc cctccgggtc gcgaaagcgc ggcgacacgg
     1141 cgtcgagcag ctcggggtcg agctcggcga ggagcccctc ttcggcgacg gagcccagcg
     1201 cgcccgcgtc ctgcgcgaag aagacgtcgg ccggggagcc gtcgccctcc tccgcgatcg
     1261 tcgccgcgag ctcggcgctg tcgccgtagc ggaccgcgac ctcgatcccc gtgtcctcgc
     1321 tgaactgctc gaggagcggg gcgacgaggc cctcgctccg gccggagtag acggtcagcg
     1381 cgtcgtcgtc cccgccgcac gcggcgagga gcgggacgag cagggcggcg gcgagcgccg
     1441 cggcggcgat tctcggcaag cgagccctcc ttgtaaggta tgccttacaa cagcgtgcgg
     1501 agggtacgcg cgggccgccg ctcccgtcaa acgggagcga tcacggcgac gcggccgtcg
     1561 tgcggccgca ccgcgacgcg cgccccgagc gggacgtgct ccgtcgacgg ccgctgcgag
     1621 cagacggtcg tcccgtcggc gaggcggacg cggtagaaga cgtcgtgccc gcggaactcg
     1681 cgcgccacga cctccccctc cccggcctcg tcggggacga gctcgaggag ctccgggcgc
     1741 aggacgacgt ccacggtggc ggcgccgttc gccgcgggcg ccgggaacgc gccgaagagc
     1801 gtccggacga ggccgtcctc gacctggccg ggcaggacgt tcgacgcgcc gacgaactcg
     1861 gccgaccagc gggaggacgg ctcgtagtag acctgctccg gcgtgccggt ctgctcgatc
     1921 cgcccgtcgt gcatgagcgc gatgcggtcg gcgacggaga acgcctcttc ccggtcgtgc
     1981 gtgacgagga ggacggtgac gccggccccg cggaggatct ccgcgagctc gtcgcgcatc
     2041 tccgagcggc gcagcgggtc gatgttcgac cacggctcgt cgaggaggac cacctcgggc
     2101 tccggcgcga gcgcgcgcgc gagggcgacg cgctgctgct ggccgccgga gagcgagtgc
     2161 gggtagcggc gctcgaggcc gccgagcccg acgagctcga gcgcctccgg cacgcgggcg
     2221 ccgcggcggg cgcgcggcag gccgaagccg acgttctccg cgacggtcag gtgcgggaag
     2281 agcgcgtagt cctggaagac catcgccgcc tggcgccgct ccggcgggac ccacgcgccc
     2341 ggccccgctg cgaggcggcc gccgaggcgg atcgagccct catcgggcgt ctcgaacccg
     2401 gcgatcaggc gcaggagcgt ggtcttcccg cagccggacg ggccgagcag ggcgacgaac
     2461 tcgccgcgcg cgagcgtgag cgaggcccgg tccacggctg cgacggcgcc gaagcgcttg
     2521 gtgactgcct cgaccgcgat catcggctcg ctcatctggc agggggacct tacagattcg
     2581 cgcccggcgt gtgccaccat gcggccgtgc tccgcgcgac ccgtctcgcc ggcgtcacgg
     2641 cgctcctcgt cgcacccgcc ccggcggccg cgggcacggc gccgctcgtc tgcctcgacc
     2701 ccggccacgc gagccgtccg aacctcacga ccgagccgat cggcccgggc tcgagcatcc
     2761 ggaagatcaa ggacggcggc ggcgcggcgg gcgaggcgcg cgtcgtcctc cggatcgcca
     2821 agcgggcccg gatcgtgctc gagaacgggg gcctccgcgt ggcgatgacg cgcaccggcc
     2881 ccgacttcac gctcggccgc ggcgggaacg tcgaccgggc gaagttctgc aaccgccgga
     2941 acgcggcgct catgatccgg atccacgccg acggctcgac cgaccccgcg cggcacggcg
     3001 cggcgacgct ctaccccgcc tggcggcgcg gctggacgga cgacgtcttc cgcccgagcc
     3061 tgcgcgcggc gcggctcctc cagcgggcgc tcgtcctgcg cacgggcgca cgcgacctcg
     3121 gcctcttccg ccgcggcgac ctcacggggt tcaactgggc gaacgtcccc gtcgtgctcg
     3181 tcgagaccgg cttcatgacg aacccggccg agcggcggcg gctcacgtcg ttcccgtacc
     3241 agtggaagat cgcgcgggcc ctcgcgcgcg gcgccgagcg gttcctcggc cgccgggcct
     3301 agctcgtctc gaggatctcc ttgagcgtgg cgaggtcctc ctcgagctgc ttctgcgcgt
     3361 gcccggtgac gacgccggcc atgaggcggg cgagcgcgct cttgtactcc gtctccatgc
     3421 gggtcgtgag gcgtgtgccg ccgttctcgc cgggcgcgag cagctggatg accttggact
     3481 cgaacgcgct cgccttgagg tcgatcacga gcttctcgtg cggctcccac tcgatgatct
     3541 ccgcgtcgat ctcgaagcgc tgcccgtggt cctcgaagac gtccttgaac ttcgtcccga
     3601 gcccgaccgg cccctcgctc acctgctcgg actccttgag cgccgacatc cagcgctgcc
     3661 gccgctcgct cgccgccagg taggcgaaga cgtgcggcgc gggccggtcg atctcgatcg
     3721 tgtgctcgtt cgtgtggacg ctcatgcgtt ctccgcagcc tcctcgtcgt cttccgtccg
     3781 ccgcatctcc tccgccgccg cccggccctg ctcgtggacg cggcgccggg cggcctcgac
     3841 gtcgccctcc tcctcggcga cgatcgtctc ggcgcccatg cccgccgcct cgaaatcgct
     3901 ctcgtcggcg gcctcctcgc gggcctgcgc aagcttctcg cggagctcgg cggcgcgcgg
     3961 gtccggcacg tcgcgcggcg cctcgcgggc cgcccgccgg ccgcgcagga cgaccgccgc
     4021 gacggccccg gcgacggctc cggcaaggag tgctgccttc tgtttcacag tcggcactct
     4081 tccatgatga agaggacgcg gcaaccgggt cgcacgtcgc tatactcgcc ggacttcctg
     4141 cttcgccggg ctccgcctga tcccgggcgg cgcgacgggc gaggccgcta gaccgaggga
     4201 agagaggagg aagggacctg aacccctacg agatcatgct gatgctcgac gccgagctcg
     4261 ccgacgaacg ccagaacgag atcctcacgc gcgcccgcga gctcgtggag cgcggcggcg
     4321 gcacctgggt cggccaggag tcctggggcc ggcgcaagct cgcgtacgag atcgacaaga
     4381 agaccgaggc gcactaccac ctgctgcact tcgactgcga gccggagacc ctggacgagc
     4441 tcacgcgcgt cctcaagatc accgacggcg cgatgcggca catggcggtg cgcaggccgg
     4501 ccgggcgcac gaccccggcg gccgctgcgg cgccctcggc cgacacggct cccgagctcg
     4561 agccggtcgg gacgcccgag acggagacga ccaccgagag c
//
LOCUS       DASTFC010000350         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_950350,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000350 DASTFC010000000
VERSION     DASTFC010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950350"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFO88_10390"
     CDS             complement(<1..226)
                     /locus_tag="VFO88_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9545972.1"
                     /translation="MPRAPRIQVPGGIHHVTARGVRRLPLYTCPDDYRLFLDVLGLVA
                     GRFGWRILAYCLMPNHYHLVVETPEPNLSAG"
     gene            complement(362..1462)
                     /locus_tag="VFO88_10395"
     CDS             complement(362..1462)
                     /locus_tag="VFO88_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9545973.1"
                     /translation="MALMPGRDPREIEEAVPHQLRLAAAIGWRVIVVVVAIGLAAFAL
                     AQVRLVVLPVVFALFLASALIQPVRGLQRLHFPPAAAVATALVVTFVVLGGLVTLLAP
                     HVAGQFGDVRDNVREGTVQVTDWLLEGPLDLSRSDLERYREQALDELEERSGDIAGGV
                     IGGAYLAVEIIAGVLLTLVLAFFFLKDGQRMWPWVVGLFPPLARARVDDVGQIAWATL
                     GGYLRGVTIVALFDAVLIAIALWVIGVPLVLPLALLTFVGGFFPIVGAFTAGAAAALV
                     ALVSNGLVDALLVVAAVVAVQQIEGNVLHPVVVSRAVKIHAVGVLLAVTAGAVIWGIV
                     GAFLAVPVVAVISRAASHLRSGGDEAVEPQPP"
     gene            1501..2370
                     /locus_tag="VFO88_10400"
     CDS             1501..2370
                     /locus_tag="VFO88_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9545974.1"
                     /translation="MGNENERDAERFSDWRELRGSGRRMIGTGVRSFAENDLLSYASA
                     IAFQVLFALIPLALSAVALLGFLGLEEVWESDIAPQVQERVQADTFSVIDRLAEQILG
                     EKRGLWLTLGAAFALWQVSGAIRATMTPLNTIYGSDERRSWWQRLLVSFALALAIGPL
                     VIGAALLIQAGPRLVRALELPGVLAVLAHVARWGIALALLATAVWLLIRYATADRHPL
                     AWSGVGTVFVVGSWILASLAFGLYATYVADYGSVFGGLATIWVLLTYLYLSSLALLFG
                     IQLDSCVRSEATD"
BASE COUNT          365 a          909 c          909 g          335 t
ORIGIN      
        1 tcccggcgga caggttcggc tccggcgtct cgacgacgag gtggtagtgg ttcggcatga
       61 ggcagtaggc gaggatccgc cagccgaagc gcccggcgac gaggccgagg acgtcgagga
      121 acagccggta gtcgtccgga caggtgtaca gcgggaggcg tcgcacgccg cgggcggtca
      181 cgtggtggat cccgccggga acctggatac gaggcgctcg gggcatgctt cgaggctaga
      241 cggaggcggg ccccgaatcg gccggcgcgc gcgaatcggc acgccttcgt gcgcctcttg
      301 tgacacgtcc ggtgccaggc accgggcgcg tccgccgcgg acatggctcc tcgggccggg
      361 gctagggcgg ctggggctcg accgcctcgt cgccgcccga ccggaggtgc gacgcggccc
      421 gcgagatgac cgcgacgacc ggcacggcga ggaacgcgcc gacgatcccc cagatcaccg
      481 cgcccgcggt cacggcgagg agcaccccga ccgcgtggat cttcaccgcc cggctgacga
      541 ccaccggatg gaggacgttc ccctcgatct gctgcaccgc caccacggcg gccacgacga
      601 gcagcgcgtc cacgaggccg ttcgagacga gcgcgacgag cgccgcggct gcgccggcgg
      661 tgaacgcccc gacgatcggg aagaacccgc ccacgaaggt gaggagcgcg agcggcagga
      721 cgagtgggac gccgatcacc cagagcgcga tcgcgatcag caccgcgtcg aagagcgcga
      781 cgatcgtcac cccgcggagg tagccgccga gcgtcgccca cgcgatctgc cccacgtcgt
      841 cgacgcgcgc ccgcgcgagg ggcgggaaga gcccgacgac ccagggccac atccgttgcc
      901 cgtccttgag gaagaagaac gcgagcacga gcgtgagcag gacgccggcg atgatctcga
      961 ccgcgaggta ggcgccgccg atcacccctc ccgcgatgtc gcccgaccgc tcctcgagct
     1021 cgtcgagcgc ctgttcgcgg taccgctcga gatcgctccg cgagaggtcg agcggcccct
     1081 ccaggagcca gtcggtcacc tgcacggtcc cctcgcggac gttgtcacgg acgtccccga
     1141 actgccccgc gacgtgcggg gccagcagtg tgacgagccc gccgagcacg acgaacgtca
     1201 cgacgagcgc ggtggccacc gccgccgctg gcgggaaatg gagccgctgc agcccgcgta
     1261 ccggctggat gagcgcggac gcgaggaaga gcgcgaacac gacggggagg acgacgagcc
     1321 gcacctgcgc gagcgcgaac gcggcgaggc cgatcgcgac gacgacgacg atcacccgcc
     1381 agccgatcgc cgccgcaagg cggagctggt ggggaaccgc ctcctcgatc tcccgcgggt
     1441 cgcggccggg catcagcgcc atcgtgggtt catacccgcc gagccggata ccgttgcccg
     1501 atggggaacg aaaacgagcg cgacgccgag cgcttctccg actggcgcga gctgcgcggc
     1561 agcggccggc gcatgatcgg cacgggtgtg cgctcgttcg ccgagaacga cctcctgagc
     1621 tacgcgagcg cgatcgcgtt ccaggtgctc ttcgcgctca tcccgctcgc gctctcggcc
     1681 gtggcgctcc tcggcttcct cggcctcgag gaggtctggg aatcggacat cgcaccgcag
     1741 gtccaggagc gggtgcaggc ggacacgttc tccgtcatcg accgcctggc ggagcagatc
     1801 ctcggcgaga agcgggggct ctggctcacg ctcggggccg cgttcgcgct ctggcaggtc
     1861 tcgggcgcca tccgggcgac gatgacaccg ctcaacacga tctacggctc cgacgagcgg
     1921 cgctcctggt ggcagcgcct gctcgtctcg ttcgcgctcg cgctcgcgat cggcccgctc
     1981 gtcatcggcg cagcgcttct gatccaggcc ggaccgaggc tcgtccgggc gcttgagctg
     2041 ccgggcgtgc tcgccgtgct cgcgcacgtc gcgcgctggg ggatcgcgct cgcgctcctc
     2101 gccacggcgg tctggctgct catccgctac gcgacggccg accggcatcc gctcgcgtgg
     2161 agcggggtcg gcaccgtctt cgtcgtcggg tcgtggatcc tcgcgtcgct cgcgttcggc
     2221 ctctacgcca cgtacgtcgc ggactacggc tccgtgttcg gcggcctcgc gacgatctgg
     2281 gtgctcctca cctatctcta tctctcgtcg ctcgcgctgc tcttcggaat ccagctggac
     2341 tcgtgcgtgc gcagcgaggc caccgactaa gggccggggc cggcgccgcg ctcgcggagc
     2401 cggtcgagga tccacgtggc gtaccacgtg ggccagtcct cgtcgcgctc gccgagctcc
     2461 ttctcgtagg cgacgtgcgc ctgctcggcc tcgcgcagga gccgggcgag ctcgtcgg
//
LOCUS       DASTFC010000351         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_27902,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000351 DASTFC010000000
VERSION     DASTFC010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27902"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFO88_10405"
     CDS             complement(<1..264)
                     /locus_tag="VFO88_10405"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9545975.1"
                     /translation="MDCDVAVLGGGPGGYSAAIRAAQLGAKTVCIEKEPELGGTCLRV
                     GCIPTKAWVQTAYALKEAEESFAKFGVNVGEPELDLKVANEWKR"
     gene            complement(277..2046)
                     /locus_tag="VFO88_10410"
     CDS             complement(277..2046)
                     /locus_tag="VFO88_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9545976.1"
                     /translation="MFLLPSIFLGIAFALLLGGKPSRLLSVEFRHGWTVFAALGLQVV
                     LFSGARDLFPPALHEPLHLSTYALLFTFAFVNVRHLALLPLFLGMALNAIAITANGGS
                     MPVKPDAWEAAGLGAGHSNNVEVGADRLAFLGDVFALPQALPLTNVFSIGDLLIGIGC
                     VVFIVAISTSEGSRRILSPSRLVEPLRIPSFRRLVVGKLVSQLGDWLTLAALIGWIYH
                     ETGSLGQVALLLLVRLAPPIVGGGLAAALVDSFPRQRLLVFVEVARGVLVAVALVSVL
                     LTIHPLAFAAIAGSGLLAAISSATTRALVPSILRRDQFAAGNAALGFAQDGAMALGAL
                     GAGIALTAASAATALAIDLVTFVVAATLYAGVRALPVPTAQVVSARGLLDGVRYVARR
                     RILLVVILSFGAATVATGLTNATLPRFLGSELGLGPGAYGFGLAALAGGLALGGAVTG
                     LVPIGEIGGRWIGVALLLMAMIFGALGLTEHAPTALLFLAFIGFLDGTTDVLFDTIVQ
                     READPSYYGRVFGLGSAFFTATMMGAVALAPFANGLGAPHQIIVIASVGLLVAAVVAL
                     VGTRERGRPSGSSAGRIALDTRP"
     gene            2459..>3340
                     /locus_tag="VFO88_10415"
     CDS             2459..>3340
                     /locus_tag="VFO88_10415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9545977.1"
                     /translation="MATVSNLRHTSVYGVAVPVLAAGTTVLAAATYALIASAPAPAVL
                     AGLGALLLAATFAEVFPVPLEPAGEVTLSAVFIAGAALVYGWDGAVVVAVLSVVAADA
                     WSGRQPIVLAYNGAAYGVSGAAAGGAVALVSHGSEVATLIFGTIAAALAFYALNMLLT
                     TAVIARFTGQNFPTLLRTNAATNAAPFAIMASMSLMLAVLWNQSPLLMGALVGPLVAV
                     ALYQRSMHRAMEAMRLALTDAQTGLGNKRHFEELLQRYLDQADETGRPLTLCLVDLDD
                     FKSVNDTFGHPAGDKVLS"
BASE COUNT          527 a         1184 c         1215 g          414 t
ORIGIN      
        1 gcgcttccac tcgttcgcga ccttgaggtc gagctccggc tcgccgacgt tcacgccgaa
       61 cttggcgaag ctctcctcgg cctccttgag cgcgtaggcg gtctggaccc aggccttcgt
      121 cgggatgcac ccgacccgga gacatgtgcc cccgagctcc ggctccttct cgatgcagac
      181 ggtcttggcg ccgagctggg ccgcgcggat cgctgcgctg tagccgcccg gaccgccgcc
      241 gagcacggcg acgtcacagt ccatgtgttc tcctcgtcag ggtcgcgtgt cgagggcgat
      301 cctaccggcc gaggagccgg agggccgacc acgctcacgc gtgcccacga gggccacgac
      361 cgcggcgacg aggagcccga cggacgcgat cacgatgatc tgatgcggcg ccccgaggcc
      421 gttcgcgaac ggcgcgagcg cgaccgcgcc catcatcgtg gcggtgaaga acgccgaccc
      481 gagcccgaag acgcgtccgt agtacgacgg atccgcctcc cgctggacga tcgtgtcgaa
      541 gaggacgtcg gtcgtcccgt cgaggaagcc gatgaaggcg aggaagagaa gcgccgtcgg
      601 cgcatgctcc gtgagcccga gcgcgccgaa gatcatcgcc atgaggagga gcgcgacccc
      661 gatccagcgc ccgccgatct ccccgatcgg cacgagcccc gtgaccgccc cgccgagcgc
      721 gagcccgccg gcgagggcgg caagcccgaa gccgtacgcg cccgggccga gcccgagctc
      781 ggacccgagg aaccgcggga gcgtcgcgtt cgtgaggccg gtcgcgaccg tcgccgcgcc
      841 gaacgagagg atcacgacga gcaggatccg ccggcgggcg acgtagcgca cgccgtcgag
      901 gaggccgcgg gccgagacga cctgcgcggt cggcacgggc agcgcgcgca cgcccgcgta
      961 gagcgtggcc gcgaccacga acgtgacgag gtcgatcgca agcgccgtcg ccgcgctcgc
     1021 ggccgtcagc gcgattccgg ccccgagcgc gccgagcgcc atcgcgccgt cctgcgcgaa
     1081 cccgagcgcc gcgttcccgg ccgcgaactg gtcgcggcgg agaatcgacg gaacgagcgc
     1141 gcgcgtcgtc gccgaggaga tcgccgcgag cagcccggac ccggcgatcg ccgcgaaggc
     1201 gagcgggtgg atcgtgagga ggacgctcac gagcgcgacc gcgacgagca cgccgcgcgc
     1261 cacctcgacg aagacgagca gccgctggcg ggggaagctg tcgacgagcg ccgcggcgag
     1321 cccgccgccc acgatcggcg gcgcgagccg cacgaggagg agcagggcga cctgcccgag
     1381 cgacccggtc tcgtggtaga tccagccgat cagcgccgcg agcgtcagcc agtcgccgag
     1441 ctgcgagacg agcttgccga cgacgaggcg gcggaacgac gggatccgga gcggctcgac
     1501 gagccgcgag ggcgagagga tccgccgcga cccctcggag gtcgagatgg cgacgatgaa
     1561 gacgacacag ccgatcccga tcaggaggtc gccgatcgag aagacgttcg tgagcgggag
     1621 cgcctgcgga agtgcgaaga cgtccccgag gaacgcgagc cggtcggcgc caacctccac
     1681 gttgttcgag tggccggctc cgagcccggc cgcctcccac gcgtcgggct tcaccggcat
     1741 gctcccgccg ttggccgtga tcgcgatcgc gttcagggcc atgccgagga acagcgggag
     1801 cagggcgagg tgccggacgt tcacgaacgc gaaggtgaag aggagcgcgt acgtcgagag
     1861 atggagcggc tcgtggagcg cgggcgggaa gaggtcgcgc gcgcccgaga agaggacgac
     1921 ctggaggccg agcgctgcga agaccgtcca gccgtgacgg aactcgacgg agagcaggcg
     1981 cgacggcttg ccgccgagga ggagcgcgaa cgcgatgccg agaaagatgc tcgggagcag
     2041 gaacatttag atctcgtcct tcagcaggcg cggcgaggga ggtgacgtgg ccggcccgaa
     2101 gaacgggccg gccacatcgg ggcgcagggg gttacgcccc ccacttgaag cccgcgcccg
     2161 cggcgagaac gaacgccgcg aggagcgcgt agcggaaagc ccaggtgcga agccgcagca
     2221 tgctttcctc cttatcgagc tcgatgggct tgacgcgcgt acgggggtcg ctcgcatcga
     2281 gcgtcgccaa tgcgaggtgc cgcacacggc gtaaccgtcg tcgcccgtgc ccgagcgggc
     2341 gatccctcga aggagtcttt cccctagtca acgtgcccgg tccgggggaa cccccttcca
     2401 agggatccgc aggcccccga aagcgcgccg atatccgcgg cagtaccacg ggtccctcat
     2461 ggccactgtc tcaaatctgc ggcatacctc ggtctacggc gtcgccgtcc ccgttctggc
     2521 ggccggcacg accgtgctcg cggctgcgac gtacgcgctg atcgcgtccg cgccggcccc
     2581 ggccgtcctg gccggcctcg gcgcgctcct gctcgcggcc acgttcgcgg aggtcttccc
     2641 ggtgccgctc gagcccgcgg gcgaggtgac gctctccgcc gtcttcatcg cgggcgcggc
     2701 gctcgtgtac gggtgggacg gcgcggtcgt cgtcgccgtc ctctcggtcg tcgcggcgga
     2761 cgcgtggtcg ggccggcagc cgatcgtgct cgcctacaac ggcgcggcgt acggcgtctc
     2821 gggcgccgcg gcgggcggcg cggtcgccct cgtctcgcac ggctcggagg tggcgacgct
     2881 gatcttcggg acgatcgcgg cggcgctcgc cttctacgcg ctgaacatgc tcctgacgac
     2941 ggccgtgatc gcccgcttca ccggccagaa ctttccgacc ctgcttcgca cgaacgcggc
     3001 gacgaacgcg gcaccgttcg cgatcatggc ctcgatgagc ctcatgctcg cggtgctctg
     3061 gaaccagtcg ccgctgctca tgggcgctct cgtcggcccg ctcgtcgcgg tcgcgctcta
     3121 ccagcgctcg atgcaccgcg cgatggaggc gatgcggctc gcgctcacgg acgcgcagac
     3181 cgggctcggc aacaagcgcc acttcgagga gctgctccag cgctacctcg accaggccga
     3241 cgagacgggc cggccgctga cgctctgcct cgtcgacctc gacgacttca agtcggtgaa
     3301 cgacacgttc gggcacccgg ccggggacaa ggtgctctcc
//
LOCUS       DASTFC010000352         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1280795,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000352 DASTFC010000000
VERSION     DASTFC010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280795"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..715
                     /locus_tag="VFO88_10420"
     CDS             149..715
                     /locus_tag="VFO88_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545978.1"
                     /translation="MDTWIWIVVAVVVVALVVAAVLAWQAARRKRLQETFGPVYEREV
                     ESASSRREAEAELEDRRKRREELDIRPLSPDAADRYARAWEGVQTQFVDDPVGATRDA
                     DRLVQEVMAERGYPMEDFDSRADLISVDHPTVVENYRSAHDAYLAYDRGDSTTEDLRQ
                     AMVHYRSLFSELLETDGRDRTTDYEEVR"
     gene            715..1155
                     /locus_tag="VFO88_10425"
     CDS             715..1155
                     /locus_tag="VFO88_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545979.1"
                     /translation="MERENRPTTADIAGREPVADDRTPGTDTDTDTRADALEQQLGDE
                     RREPVGETAEPAENRRLLEDDATSDVQTRWREIQGSFVDEPRRAVEDADALVADLMQR
                     LADGFAQERSRLESQWDRGDEVSTEDLRVALQRYRSFFDRLLSA"
     gene            complement(1203..2345)
                     /locus_tag="VFO88_10430"
     CDS             complement(1203..2345)
                     /locus_tag="VFO88_10430"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET9545980.1"
                     /translation="MPDHPSHTAVGGVLSQVASALERLSVETTPVALLTAAARELRER
                     LGTRAVVVSRREGERLREVACSLHEDQSDRTAFSDYAYLLSDYPLTQKVLDTGRPVAF
                     SLHDADIDPNEAFVLREMRMDAVLMTALPAGGDVWGLVEVYDTGVRRFDADEVALADF
                     LVRQVGALLVGLEHTESVQRLYRETLASLANALEAKDSYTHDHTQEVVGLTLDVGRAL
                     GLDDTELDVLELGALFHDIGKIRVPEAILNKPGPLDDEEWGVMRRHTVAGESILAPIT
                     SLRAVLPIVRSSHERWDGRGYPDGLAGRQIPLGARIVSVCDAYRAMIERRSYREPMTQ
                     REALGELRDNAGTQFDPECVRALMSVLVTRGSDEEQVPLHRPAHAA"
     gene            2455..2527
                     /locus_tag="VFO88_10435"
     tRNA            2455..2527
                     /locus_tag="VFO88_10435"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2489..2491,aa:Glu,seq:ctc)
BASE COUNT          407 a          965 c          959 g          354 t
ORIGIN      
        1 gccacgaaga ggatccgcag aaccctcacg gccgctcagg tgcccgaaat cacagcggag
       61 aaacctcctt cgcatacgag ccgaccggcg tgggtagaga ggccgggtca tccgtttccg
      121 gtcgagcgcc ggaacgaaag gagcaagcat ggacacttgg atctggatcg tcgtcgcagt
      181 ggtcgtggtg gcgctcgtcg tcgccgcggt gctcgcgtgg caggcggccc gccggaagcg
      241 gttgcaggag acattcgggc ccgtttacga gcgcgaggtc gagtccgcgt cgtcccggcg
      301 agaagccgag gcggagctcg aggaccgtcg gaagcgccgc gaggagctcg acatccgccc
      361 gctgagcccg gacgccgccg accggtacgc gcgcgcatgg gagggcgtgc agacgcagtt
      421 cgtcgacgac cccgtcggcg cgacgcgcga cgccgaccgc ctcgtccagg aggtgatggc
      481 cgagcgcggc tacccgatgg aggacttcga cagccgggcc gacctcatct cggtcgacca
      541 cccgaccgtg gtcgagaact accgctcggc ccacgacgcc tatctcgcct acgaccgggg
      601 cgactcgacg accgaggatc tccggcaggc gatggtgcac taccggtcgc tcttcagcga
      661 gctgctcgag accgacggcc gggatcggac caccgactac gaggaggtgc gctgatggag
      721 cgcgagaatc gtcccacgac cgcagacatc gccggccgtg agccggtcgc cgacgaccgc
      781 acgcccggaa ccgacaccga caccgacacg cgcgcggacg cgctcgagca gcagctcggc
      841 gacgagcggc gcgagcccgt cggcgagacc gcggagcccg cggagaaccg ccggctgctc
      901 gaggatgacg cgacgagcga cgtccagacc cgttggcgcg agatccaggg cagcttcgtc
      961 gacgagccgc gccgcgccgt cgaggacgcc gacgccctcg tcgccgacct catgcagcgg
     1021 ctcgcagacg gcttcgcgca ggagcggagc cgcctcgaga gccagtggga ccgcggcgac
     1081 gaggtctcga cggaggatct ccgcgtcgcg ctgcagcgct accggtcgtt cttcgaccgc
     1141 ctgctctccg cctgacgcct cacccgcgcg ggtcggccgc ccggtcggcc cgcgcgaccg
     1201 cgctacgccg cgtgcgcggg gcggtggagc ggcacctgct cctcgtccga gccgcgcgtc
     1261 acgagcacgg acatgagggc ccgcacgcac tccgggtcga actgcgtccc cgcgttgtcg
     1321 cggagctcgc cgagcgcctc gcgctgcgtc atcggctcgc ggtacgagcg ccgctcgatc
     1381 attgcgcggt aggcgtcgca cacggagacg atccgcgccc cgagcgggat ctgccggccg
     1441 gcgagcccgt ccgggtagcc gcggccgtcc cagcgctcgt ggctcgagcg gacgatcggc
     1501 agcaccgcgc ggagcgacgt gatcggcgcg aggatcgact cccccgccac cgtgtgccgc
     1561 cgcatgacgc cccactcctc gtcgtcgagc gggcccggct tgttcagaat cgcctctggc
     1621 acgcggatct ttccgatgtc gtggaagagg gctccgagct cgagcacgtc gagctccgtg
     1681 tcgtcgagcc cgagcgcgcg cccgacgtcg agcgtcaggc cgacgacctc ctgcgtgtgg
     1741 tcgtgcgtgt aggagtcctt cgcctcgagc gcgttcgcga gcgacgcgag cgtctcccgg
     1801 tagaggcgct gcaccgactc ggtgtgctcg agcccgacga gcagcgcgcc cacctggcgc
     1861 acgaggaagt ccgcgagcgc cacctcgtcc gcgtcgaagc ggcggacgcc cgtgtcgtag
     1921 acctcgacga gcccccagac gtcgccgccg gcggggagcg cggtcatgag caccgcgtcc
     1981 atccgcatct cgcgcaggac gaacgcctcg ttcgggtcga tgtccgcgtc gtggagcgag
     2041 aacgcgaccg gccgaccggt gtcgagcacc ttctgcgtga gcgggtagtc cgagaggagg
     2101 tacgcgtagt cgctgaacgc cgtccggtcg ctctgatcct cgtgcagcga gcaggcgacc
     2161 tcgcgcagac gctcgccctc gcgccgcgag acgaccaccg cccgcgtgcc gagccgctcc
     2221 cggagctccc gcgcggcagc cgtgagcagc gccacgggcg tcgtctcgac gctcaggcgc
     2281 tccagcgcgc tcgccacctg cgagagcacg ccgccgacgg cggtatgaga ggggtggtcg
     2341 ggcatgacgg ggggcaggac gaagcctgct gactggaggc cgaaagagcc tccccctttc
     2401 ggggggaaca ccgtgcgacg agctcggcgt cggatgctag gctgcgagcc gcacggcgca
     2461 ttcgtctagg ggcctaggac gccgccctct cacggcggta acacgggttc gaatcccgta
     2521 tgcgcctctc ctgcgaaggc tggtaaccgg ggtccccatg agagatcccg gacagcctcc
     2581 atggccttct cagtgtcgag ccgccgcagg taggtctgcg tcgtctctag gcgcgcgtgc
     2641 ccgagcagcg cctggagcgc gtcgagccgg cccgggttcg attcg
//
LOCUS       DASTFC010000353         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_440169,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000353 DASTFC010000000
VERSION     DASTFC010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440169"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VFO88_10440"
     CDS             <1..1683
                     /locus_tag="VFO88_10440"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9545981.1"
                     /translation="EYELGIQDQAFLGPESGLAVPDGDGGVDIYVATQWLHVDRAQVA
                     PCLDLPPERVRIHLAGVGGAFGGREDLSMQIHGALLALHTNRPVKIVYTREESFVGHV
                     HRHPARIRMEHRANRDGKLVCVHARVLLDGGAYASSSTAVVTNASSFACGPYAVANAL
                     VDATVVYTNNPPCGAMRGFGAVQVCFAHEAQMDKLAAALELDPVELRLRNALAPGDVM
                     PNGQKVTGSLPVADVIRRCAELPVPEPEPLPRDSLRLPGGSGNVTRGEGVKRGVGFAL
                     GFKNLCYSHGFDDYCAARVRLFAGADGALAAEVHCAAAEVGQGVVGVITQVARTELEL
                     DDVTLAPGTTSTVDSAGSASASRMTWMASGAVAEACRAARAELGARGGTLAPGEEIDV
                     EKVYRHPQTWPLDPETGQVTGERAHVAFICSAMRAVVEVDTDLGLTRAVWIGTAQDVG
                     RAVNPQAVLGQIEGGTAQGLGLALMEEIQTRDGLITNASFTDYLIPTTLDMPPVDTVL
                     VEEPEPDAPYGAKGVGEPPTVVATAAVAAALRDATGRDLTRVPVRPDDLIGL"
     gene            complement(1680..2132)
                     /locus_tag="VFO88_10445"
     CDS             complement(1680..2132)
                     /locus_tag="VFO88_10445"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9545982.1"
                     /translation="MAGGTAAAVVVCVVAGLAGAIQAAVMGRFGERIGVVEAVAFAAL
                     VTALVAFASLLVVRQSASGVAEGLRAPPWMWLAGVMSAFIVFAVALGPPRIGTTTTIG
                     LIIAGNLVMAAVIDRYGLFGLERIGLSPIRIVGITLLGVGSALTLYRP"
     gene            2203..3015
                     /locus_tag="VFO88_10450"
     CDS             2203..3015
                     /locus_tag="VFO88_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9545983.1"
                     /translation="MSQPTYPSPVDPAELGFEKIVYEKAPPRATITLNRPDILNAFDF
                     QMLRELARACEDASYDDDIRVVVLTGAGRAFCVGADLKSWSQEYLGKPNEYWKWFGAF
                     KDAHDRLREIGKPALARINGICVGGGNELQMACDLAVMVDDAFIRHVGPEHGSVPAGG
                     ATQWLSIIVGDRRAREIVLMCEEIPAAKAAEWGLVNWAVPAAELDAKVDAVVENLVRK
                     LPQTTRYAKQHLNFWKDLAWHETINHARDWLALSMATEEPQAAVRKFVSKDK"
BASE COUNT          456 a         1112 c         1135 g          380 t
ORIGIN      
        1 gagtacgagc tcgggatcca ggaccaggcg ttcctcggcc ccgagtcggg gctcgcggtg
       61 ccggacggcg acgggggcgt cgacatctac gtcgcgacgc agtggctcca cgtcgaccgg
      121 gcgcaggtcg cgccgtgcct cgacctgccg ccggagaggg tgcgcatcca cctcgccggc
      181 gtcggcggcg cgttcggcgg gcgcgaggac ctctcgatgc agatccacgg cgcgctcctc
      241 gcgctgcaca cgaaccggcc cgtgaagatc gtctacaccc gcgaggagtc gttcgtcggc
      301 cacgtgcacc ggcacccggc gcggatccgg atggagcacc gcgcgaaccg ggacgggaag
      361 ctcgtgtgcg tgcacgcgcg cgtgctcctc gacggcggcg cgtacgcgtc gagctccacc
      421 gccgtcgtca ccaacgcgtc ctcgttcgcg tgcggcccgt acgcggtcgc gaacgccctc
      481 gtcgacgcga cggtcgtcta cacgaacaac cctccgtgcg gcgccatgcg cggcttcgga
      541 gccgtgcagg tgtgcttcgc ccacgaggcg cagatggaca agctcgcggc cgcgctcgag
      601 ctcgacccgg tcgagctccg gctgcggaac gcgctcgcgc caggcgatgt catgccgaac
      661 ggccagaagg tcacgggatc gctgccggtc gccgacgtca tccgccgctg cgccgagctc
      721 ccggtgcccg agccggagcc gctgccccgc gactcgctcc ggcttcccgg cggctccggg
      781 aacgtgacgc gcggcgaggg ggtgaagcgc ggcgtcgggt tcgcgctcgg gttcaagaac
      841 ctctgctact cgcacggctt cgacgactac tgcgccgcgc gcgtgcgcct cttcgccggc
      901 gcggacgggg cgctcgcggc cgaggtgcac tgcgcggccg ccgaggtcgg gcagggcgtc
      961 gtcggcgtga tcacgcaggt ggcgcggacg gagctcgagc tcgacgacgt gacgctcgcg
     1021 ccggggacga cctcgacggt cgactcggcg ggctcggcgt ccgcctcgcg catgacgtgg
     1081 atggcgtccg gcgccgtggc cgaggcgtgc cgggcggcgc gggctgagct cggcgcacgc
     1141 ggcggcacgc tggcgcccgg cgaggagatc gacgtcgaga aggtctaccg ccacccgcag
     1201 acctggccgc tcgatcccga gacggggcag gtcacgggcg agcgggccca cgtcgcgttc
     1261 atctgctcgg cgatgcgcgc ggtcgtcgag gtggacaccg acctcggcct cacgcgcgcg
     1321 gtctggatcg ggacggcgca ggacgtcggc agggcggtca acccgcaggc cgtcctcggg
     1381 cagatcgagg gcggcaccgc gcaggggctc gggcttgcgc tcatggagga gatccagacg
     1441 cgcgacgggc tgatcacgaa cgcgagcttc acggactacc tcatcccgac gacgctcgac
     1501 atgccgccgg tcgacacggt gctggtcgag gagccggagc cggacgcgcc ctacggggcg
     1561 aagggcgtcg gcgagcctcc gacggtcgtc gcgaccgccg cggtcgccgc ggccctgcgc
     1621 gacgcgaccg gccgcgacct cacgcgcgtg ccggtccggc cggacgacct gatcgggctc
     1681 tagggccggt agagcgtcag cgccgagccg acgccgagca gggtgatccc gacgattcgg
     1741 atcgggctga ggccgatgcg ctcgaggccg aagaggccgt agcggtcgat cacggcggcc
     1801 atcacgaggt tcccggcgat gatgagcccg atcgtcgtcg tcgtcccgat ccgcggcggc
     1861 ccgagcgcga ccgcgaagac gatgaacgcg ctcatgacgc cggcgagcca catccagggc
     1921 ggcgcgcgca gcccctcggc gacgcccgag gccgactggc gcacgacgag gagcgacgcg
     1981 aacgcgacga gcgccgtgac gagggccgcg aacgcgaccg cctcgacgac gccgatccgc
     2041 tcgccgaagc ggcccatcac ggccgcctga atcgctcccg cgagccccgc gaccacgcac
     2101 acgaccaccg ccgccgccgt tcctcccgcc acgagccctc ctttccgccg aaccgcgggg
     2161 gaagctatac gctcgcgggc gaacgatgaa gggggacgcc gcgtgagcca gccgacgtac
     2221 ccgagcccgg tcgacccggc cgagctcggc ttcgagaaga tcgtgtacga gaaggcgccg
     2281 ccccgggcca cgatcacgct gaaccggccc gacatcctga acgcgttcga cttccagatg
     2341 ctccgcgagc tcgcgcgcgc gtgcgaggac gcgtcctacg acgacgacat ccgtgtcgtc
     2401 gtcctgaccg gcgccgggcg cgccttctgc gtgggcgccg acctcaagtc gtggtcgcag
     2461 gagtacctcg ggaagccgaa cgagtactgg aagtggttcg gcgcgttcaa ggacgcgcac
     2521 gaccgcctcc gcgagatcgg caagccggcc ctcgcccgga tcaacgggat ctgcgtcggc
     2581 ggcgggaacg agctccagat ggcctgcgac ctcgcggtga tggtcgacga cgcgttcatc
     2641 cggcacgtcg ggcccgagca cggctccgtg ccggccggcg gcgcgacgca gtggctctcg
     2701 atcatcgtcg gcgaccggcg ggcgcgcgag atcgtgctca tgtgcgagga gatcccggcg
     2761 gctaaggccg ccgagtgggg cctcgtgaac tgggccgtcc ccgcggccga gctcgacgcg
     2821 aaggtggacg cggtcgtcga gaacctcgtg cgcaagctcc cgcagacgac gcgctacgcg
     2881 aagcagcacc tgaacttctg gaaggacctc gcgtggcacg agacgatcaa ccacgcccgc
     2941 gactggctcg cgctctcgat ggcgacggag gagccgcagg cggccgtccg gaagttcgtg
     3001 tcgaaggaca agtgagcgca gaggacgcac tcgtcgtcgt cgagcgggac gacccgatcg
     3061 cggtcgtccg gctcaaccgg ccc
//
LOCUS       DASTFC010000354         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_604585,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000354 DASTFC010000000
VERSION     DASTFC010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604585"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /gene="ahcY"
                     /locus_tag="VFO88_10455"
     CDS             <1..1191
                     /gene="ahcY"
                     /locus_tag="VFO88_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9545984.1"
                     /translation="RNMPVLAAIRERFEREKPLDGYRVSACLHVTSETANLARTLKAG
                     GADMVLCASNPLSTQDDVAAALVQEYGIPVFAIRGEDDATYYSHITAAVDHRPHITMD
                     DGADVIGVLHKDRRDGLADIIGGTEETTTGVIRLRALEAQGELAFPIVAVNEAKTKHL
                     FDNRYGTGQSTIDGIIRATNVLLAGKRFVVSGYGWVGRGVAMRARGMGSHVIVTEVDP
                     MRALEALMDGFEVLPIERAAEVGDIFCTATGDKHVISRGAIERMKDGAILSNTGHFNV
                     EIDIPALEELSTGQRPQRENVVAYDLADGRTVYLIADGRLVNLSAAEGHPAAVMDMSF
                     ANQSLAAEHVVGNADSLEPKVYVVPDEIDREIARLKLETMGVDIDRLTEEQAKYLASW
                     DEGT"
     gene            1173..2168
                     /gene="mtnA"
                     /locus_tag="VFO88_10460"
     CDS             1173..2168
                     /gene="mtnA"
                     /locus_tag="VFO88_10460"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET9545985.1"
                     /translation="MGRRNVELRPEQIARLEGDAVVLLDQRRLPAEETELVCRTTDEL
                     ADAIRTLAVRGAPAIGVAAAYGYAAAAARGEDLAEAARALLASRPTAVNLAWAIRELE
                     AAGDDPAVLAARARELHREEVDRCRRMAEHALALFDAGSRPLTHCNTGGLATGGYGTA
                     LGAIRRAFEAGLVESVLVDETRPLLQGARLTAWELDRAGVPHAVIADAAAGALMARGE
                     VTHVLTGADRIAANGDTANKVGTYALAVLAAHHGLPFVVVAPSSTVDLETPTGAEIPI
                     EERDGREVTDAFPARNPAFDVTPAGLVTAIVTEDGVHRAPYGESLARPAAARSAA"
     gene            2174..>2403
                     /locus_tag="VFO88_10465"
     CDS             2174..>2403
                     /locus_tag="VFO88_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9545986.1"
                     /translation="MVMAAGLGTRLRPLTDFLPKPMMPVANRPVLHHLLNLLRRHGID
                     QVGINLHAAPGMIESYFGDGQTLDMEIRWSHEP"
BASE COUNT          360 a          904 c          850 g          289 t
ORIGIN      
        1 cggaacatgc cggtcctcgc cgcgatccgg gagcgcttcg agcgggagaa gccgctcgac
       61 ggctaccggg tctccgcctg cctgcacgtc acctcggaga ccgcgaacct cgcgcgcacg
      121 ctcaaggccg gcggcgccga catggtgctc tgcgcctcga acccgctctc cacgcaggac
      181 gacgtcgccg cggcgctcgt ccaggagtac gggatcccgg tgttcgcgat ccgcggcgag
      241 gacgacgcga cgtactacag ccacatcacc gcggccgtcg accacaggcc gcacatcacg
      301 atggacgacg gcgccgacgt gatcggcgtc ctccacaagg accggcgcga cgggctcgcg
      361 gacatcatcg gcggcacgga ggagacgacg accggcgtca tccgcctccg cgcgctcgag
      421 gcgcagggcg agctcgcgtt cccgatcgtc gccgtgaacg aggcgaagac gaagcacctc
      481 ttcgacaacc gctacggcac cgggcagtcg acgatcgacg ggatcatccg ggcgacgaac
      541 gtcctcctcg cggggaagcg cttcgtcgtc tccggctacg gctgggtcgg gcgcggcgtc
      601 gcgatgcggg cccgcgggat gggctcgcac gtgatcgtca ccgaggtcga cccgatgcgc
      661 gcgctcgagg cgctcatgga cggcttcgag gtgctcccga tcgagcgcgc ggccgaggtc
      721 ggcgacatct tctgcacggc gacgggcgac aagcacgtca tctcgcgcgg cgcgatcgag
      781 cggatgaagg acggcgccat cctctcgaac accgggcact tcaacgtcga gatcgacatc
      841 ccggcgctcg aggagctctc gaccgggcag cgtccgcagc gtgagaacgt cgtcgcctac
      901 gacctggccg acggccgcac cgtctacctg atcgcggacg ggcgactcgt caacctctcg
      961 gccgccgagg gccaccccgc ggcggtcatg gacatgagct tcgcgaacca gtccctcgcg
     1021 gccgagcacg tcgtcgggaa cgccgacagc ctcgagccga aggtctacgt cgtgccggac
     1081 gagatcgacc gcgagatcgc ccgcttgaag ctcgagacga tgggcgtgga catcgatcgt
     1141 ctcaccgagg agcaggccaa gtacctcgcc tcgtgggacg aaggaacgta gagctccggc
     1201 ccgagcagat cgcgcgactc gagggggacg cggtcgtgct cctcgaccag cggcgtcttc
     1261 ccgcggagga gaccgagctc gtctgccgca cgaccgacga gctcgcggac gcgatccgca
     1321 cgctcgccgt gcgcggcgcg cccgcgatcg gcgtggcggc agcctacggc tacgcggccg
     1381 cggccgcccg cggggaggac ctcgcggagg ccgcgcgggc cctcctcgcc tcgcgcccga
     1441 ccgccgtgaa cctcgcgtgg gcgatccggg agctcgaggc ggcgggcgac gacccggcag
     1501 tcctcgccgc gcgcgcccgg gagctgcacc gcgaggaggt cgaccgctgc cgccgcatgg
     1561 ccgagcacgc gctcgccctc ttcgacgccg gctcgcggcc gctcacgcac tgcaacaccg
     1621 gcggcctcgc gaccggcggg tacggcaccg cgctcggcgc gatccggcgc gcgttcgagg
     1681 ccgggcttgt cgagagcgtc ctcgtggacg agacgcggcc gctcctccag ggcgcacgcc
     1741 tcaccgcgtg ggagctcgac cgcgccgggg tgccgcacgc ggtgatcgcg gacgcggcgg
     1801 cgggcgcgct catggcgcgc ggcgaggtga cccacgtcct gaccggcgcc gaccggatcg
     1861 ccgcgaacgg cgacacggcg aacaaggtcg gcacgtacgc gctcgccgtc cttgccgccc
     1921 accacgggct ccccttcgtc gtcgtcgcgc cgagctccac ggtcgacctc gagaccccga
     1981 ccggcgcgga gatcccgatc gaggagcgcg acggccgcga ggtgaccgac gcgttcccgg
     2041 cgcggaaccc cgccttcgac gtgacgccgg ccggcctcgt gaccgcgatc gtcacggagg
     2101 acggcgtcca ccgcgccccg tacggcgagt ccctcgcccg cccggccgcc gcgaggagcg
     2161 cagcgtgaag gcgatggtca tggcggcggg gctcggcacg cgcctgcgcc cgctgaccga
     2221 cttcctcccg aagccgatga tgccggtcgc gaaccggccc gtcctccacc acctcctgaa
     2281 cctcctgcgg cggcacggga tcgaccaggt cgggatcaac ctccacgcgg cgccggggat
     2341 gatcgagagc tacttcggcg acggccagac gctcgacatg gagatccgct ggtcgcacga
     2401 gcc
//
LOCUS       DASTFC010000355         7718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1066736,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000355 DASTFC010000000
VERSION     DASTFC010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066736"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="coaD"
                     /locus_tag="VFO88_10470"
     CDS             <1..504
                     /gene="coaD"
                     /locus_tag="VFO88_10470"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9545987.1"
                     /translation="ICPGAYDPVTNGHIDIITRAATIFERVVVAVVREPQHKQPTFST
                     DERVAFLKEALEGVENVEVEVFSELVVEFARRWGAKTLVKGLRAISDFEWEFQMHHLN
                     RGLAPDIETMYLMSSPQYSFLSSSGVKEIASFGGTVENLVPPAVARRFAEMHPRPQAG
                     APTSPRE"
     gene            519..1010
                     /locus_tag="VFO88_10475"
     CDS             519..1010
                     /locus_tag="VFO88_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545988.1"
                     /translation="MDVLVLIDKLDDLVHNAKAVPLTDQVRIDREEIYDILDQMRATI
                     PEEIKQARWIVKERQEMLAEAKRESDRILGEAREQAVREASQTEIVKLAERQAQDIVE
                     DARRQARETRLEMEDWADSILSTLEVNLDKFLGAVRRGRERLHERSQESVMAGLSDET
                     VQQ"
     gene            1063..1575
                     /locus_tag="VFO88_10480"
     CDS             1063..1575
                     /locus_tag="VFO88_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET9545989.1"
                     /translation="MTSLDLRRLRIRPGEQHRERRAVELEPYDLGGQRYEPHPRDPEA
                     ELTLTRTMDGLVLELDLDARLAGPCFRCLTDTEVELRVRGREYQAGRGPADDEELRTP
                     YVEDERLDVSRWARDAIALALPEKILCREDCAGLCAVCGRDLNAEPHTHEETAADPRW
                     GALAELRDRL"
     gene            1602..1790
                     /gene="rpmF"
                     /locus_tag="VFO88_10485"
     CDS             1602..1790
                     /gene="rpmF"
                     /locus_tag="VFO88_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9545990.1"
                     /translation="MAVPKRKTSKSRRDKRRATHKLSAPVVIECPQCHQPKRPHRVCP
                     TCGTYRGREVEPFQAPAP"
     gene            1801..2805
                     /gene="plsX"
                     /locus_tag="VFO88_10490"
     CDS             1801..2805
                     /gene="plsX"
                     /locus_tag="VFO88_10490"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET9545991.1"
                     /translation="MSADARPVRVAVDAMGGDRAPEEIVAGALAARGPRLEPVLHGRK
                     PVVTPLADGLPVVHADEVVAMDEKPGDAARTKRGSSLFAVCRAVAEGEADVAVSAGNT
                     GALLAAGLVEIGRLPGVHRPAIAIPLPGPDGPTVLIDGGANADARPEHLVQFGHMGAV
                     FAEEALGIASPRVALLSIGEEPEKGNKLVRETHELLRATDLRYTGNVEGRDLLASAAD
                     VVVCDGFTGNVALKTLEGGIRTVMTGLREAIGAGLRTKVGGLLIRPAARELRAKLDPD
                     TYGGAYLLGLKGLVVIAHGSSSSAAAANAIRLAARGVEGRVVERVAARLAEGRAEAAG
                     "
     gene            2875..3120
                     /gene="acpP"
                     /locus_tag="VFO88_10495"
     CDS             2875..3120
                     /gene="acpP"
                     /locus_tag="VFO88_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9545992.1"
                     /translation="MAASREEIHEQVKNVLVEQLGIDEGAITEDASFQEDLDADSLDL
                     VELIMELEDQFGIKISDEDAQGITTVGQAVDYISKHQ"
     gene            3179..3820
                     /gene="rnc"
                     /locus_tag="VFO88_10500"
     CDS             3179..3820
                     /gene="rnc"
                     /locus_tag="VFO88_10500"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET9545993.1"
                     /translation="MPDGRLQHVFTHTSWAPSREDSYERLEFLGDSVLGLAIARELYE
                     RFPEYPEGQLAKIRAHVVSRQSCAVVGRRLDLGQKLIERGADSVPREELERLSVNRNV
                     LAALVEAALGALYLEHGFERIREAVLAAFADRIDYALTTYVDYKTELQEELARRSQSV
                     SYTVLEVDGPPHDRRFTCAAVIDGEQAGSGTGASKKAAEQNAAREALRSLPPV"
     gene            complement(3817..4569)
                     /locus_tag="VFO88_10505"
     CDS             complement(3817..4569)
                     /locus_tag="VFO88_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET9545994.1"
                     /translation="MTLGPLRIELCGRVVVRDGDRRLDAELPGRQGRVLLAYLVLERH
                     RPVSRNELVEAVWPDGAPAASDAALSALLSKLRRALGPARLGSGTPVALTLPADAWVD
                     VEAAAEALHRAESAVAREDWKAAWGPARVVQHVTARELLSGDDAPWIAERRRRLEEWH
                     LRALELVAEACLGIGGSELATAERAARRLVERAPYRESGHRALMRTLVRRGNPAEALQ
                     VYDRLRLRLREDLGVAPAPPTQALHRELVALG"
     gene            4631..4789
                     /locus_tag="VFO88_10510"
     CDS             4631..4789
                     /locus_tag="VFO88_10510"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET9545995.1"
                     /translation="MARVINCECGRTIRGETDEEVVANAEGHVREDHPELVGSITRDD
                     LLAMTEEA"
     gene            4789..5133
                     /locus_tag="VFO88_10515"
     CDS             4789..5133
                     /locus_tag="VFO88_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET9545996.1"
                     /translation="MARLLVNVATGPENPTRAALGLLVARTAAAEGHEVRVFLAGDGV
                     HLARPETAAATHGIGTGAAGEHLEALRAAGVPVLLSGMSSKARGIEGGGELSPPERLV
                     ELAAWADTALVY"
     gene            5173..7341
                     /locus_tag="VFO88_10520"
     CDS             5173..7341
                     /locus_tag="VFO88_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9545997.1"
                     /translation="MNLRSIRMRGFKSFPDEVEVRLEPGVAVVVGPNGSGKSNIADAV
                     VWAAGSLSPSELRAEKPEDVLFAGSASRPGADACSVELLFDNPDGDEPLPYAEVSVAR
                     RLERGGEGQYLVNGAHVRRTDLVELLADLGLGGEMHSVISQGRVEQILASKPSERRAL
                     VEEAAGLGRFKRRRHRAELKLARVATQVERALDLEAEVRKRLRPLALQATAAERAQKL
                     AGQIDELSVRLAAADLDRLDRTLGEIAGRRAAAERERKRHDERLSALLEERREAEEEL
                     GEAGGRHEQATAALYRLRSAVERAELRRERALETAKTLRAELDRPRLPLPEAPPSYEQ
                     VVRAARLWPAVPAADLDAGAELLRRAADEQRDALLVASGGETWFAADAEAKRDAPEKR
                     PGVPLAALADASERAATALERAAASAGRFEPALAARVDAGGSRSGELGERLRALGAQE
                     ADLRRAAAEAAERLTQVQVEAARLEREREDVVERLPEGHEAEGLTDDEAAELRAQVER
                     LERRREALGQVNPLAAEEHAREKERLDDLVEQRQDLERSLTELETLRAELTETVERRF
                     AETYERVEHHFAEVVATLFPGGTGRLIAVDPEEEGEEHGVEIELQPAGKRITRLSLLS
                     GGEKALGAISFLFALFLARPCPFYLLDEVEAALDDTNIGRFVELLRRFSDRAQFIVVT
                     HQKRTMEAADILYGVTMGADGVSQVVSKRLTAERAASAAA"
     gene            7338..>7718
                     /locus_tag="VFO88_10525"
     CDS             7338..>7718
                     /locus_tag="VFO88_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HET9545998.1"
                     /translation="MSRSWAEFLGDPAPASEPETGGRGGWFRRLRDSLATSREALTSE
                     LASAVFDPADDESWERIEEALLRADVGVPATVEIVRRLEERRPATQAELVEGLEEVLT
                     DLVSPAGEARLGLGGRPSVVLVVGV"
BASE COUNT         1075 a         2812 c         2913 g          918 t
ORIGIN      
        1 atctgcccgg gcgcctacga cccggtcacg aacgggcaca tcgacatcat cacgcgggcc
       61 gcgaccatct tcgagcgcgt cgtcgtcgcc gtcgtccgcg agccgcagca caagcagccg
      121 accttctcga ccgacgagcg cgtcgcgttc ctcaaggagg cgctcgaggg cgtggagaac
      181 gtcgaggtgg aggtgttctc cgagctcgtc gtcgagttcg cgcggcgctg gggcgcgaag
      241 acgctcgtga agggcctgcg cgcgatctcg gacttcgagt gggagttcca gatgcaccac
      301 ctgaaccgcg gcctcgcgcc cgacatcgag acgatgtacc tcatgtcgag cccccagtac
      361 agcttcctct cgtcgagcgg ggtgaaggag atcgcgtcgt tcgggggaac ggtggagaac
      421 ctcgttccgc ccgccgtcgc gcggcggttc gcggagatgc atccccgccc ccaggcgggc
      481 gccccgacaa gccctcgtga gtagactggc aggccggaat ggacgttctc gttctcatcg
      541 acaagctcga cgacctcgtg cacaacgcga aggccgttcc gctcaccgac caggtgcgga
      601 tcgaccgcga ggagatctac gacatcctcg accagatgcg cgcgacgatc cccgaggaga
      661 tcaagcaggc gcgctggatc gtgaaggagc gccaggagat gctcgccgag gcgaagcgcg
      721 agagcgaccg catcctcggc gaggcccgcg agcaggccgt ccgcgaggcg tcccagaccg
      781 agatcgtgaa gctcgccgag cgccaggcgc aggacatcgt cgaggacgcc cgccgccagg
      841 cgcgcgagac gcggctcgag atggaggact gggccgactc gatcctgtcc acgctcgagg
      901 tgaacctcga caagttcctc ggtgccgtgc ggcgcggccg cgagcggctc cacgagcgct
      961 cccaggagtc cgtcatggcc gggctctcgg acgagaccgt ccagcagtag gcgctgcgct
     1021 cggtcgggcg gcggcgcccc gacggttcgc taccgtgggt gcatgacgtc gctcgacctg
     1081 cgccggctcc ggatcaggcc gggggagcag caccgggagc gccgcgccgt cgagctcgag
     1141 ccgtacgacc tcggcgggca gcgctacgag ccgcaccccc gcgacccgga ggccgagctc
     1201 acgctcaccc gcacgatgga cgggctcgtg ctcgagctcg acctcgacgc acgcctcgcc
     1261 gggccgtgct tccgctgcct cacggacacg gaggtggagc tgcgcgtccg cggccgcgag
     1321 taccaggccg ggcgcggtcc ggcggacgac gaggagctcc ggacgccgta cgtcgaggac
     1381 gagaggctcg acgtctcccg ctgggcgcgc gacgcgatcg cgctcgccct cccggagaag
     1441 atcctctgcc gggaggactg cgcggggctc tgcgccgtct gcgggcgcga cctgaacgcc
     1501 gagccgcata cgcacgagga aaccgccgcg gacccgcgct ggggggcgct cgcggagctt
     1561 cgtgaccgcc tgtagcgcgt tctggctaca atctcgcgct catggcagtc ccgaagcgaa
     1621 agacctcgaa gtcccgccga gacaagcggc gggcgacgca caagctctcg gccccggtgg
     1681 tgatcgagtg cccgcagtgc caccagccga agcggccgca ccgggtctgc ccgacctgcg
     1741 gcacgtaccg cggccgcgag gtcgagccct tccaggcgcc ggccccgtag tccttggccc
     1801 gtgagcgctg acgctcggcc cgttcgcgtc gccgtggacg cgatgggcgg cgaccgcgcg
     1861 ccggaggaga tcgtcgccgg ggcgctcgcg gcgcgcgggc cgcggctcga gccggtgctt
     1921 cacggacgaa agcctgtcgt gacgccgctc gcggacgggc ttcccgtcgt gcacgcggac
     1981 gaggtcgtgg cgatggacga gaagcccggc gacgccgcgc ggacgaagcg gggaagctcg
     2041 ctcttcgccg tctgccgcgc ggtcgccgag ggggaggccg acgtcgcggt ctccgcgggg
     2101 aacacgggcg cgctcctcgc cgccgggctc gtcgagatcg ggcgcctgcc gggcgtccac
     2161 cggcccgcga tcgccatccc gctccccggc ccggacgggc cgaccgtcct gatcgacggc
     2221 ggcgccaacg cggacgcgag gcccgagcac ctcgtccagt tcggccatat gggcgccgtc
     2281 ttcgccgagg aggcgctcgg gatcgcgtcg ccgcgggtcg cgctcctctc gatcggcgag
     2341 gagcccgaga agggcaacaa gctcgtccgc gagacgcacg agctcctgcg cgcgaccgac
     2401 ctgcgctaca cgggcaacgt cgagggccgc gacctcctcg cgagcgcggc cgacgtcgtc
     2461 gtctgcgacg gcttcacggg gaacgtcgcc ctgaagacgc tcgaaggcgg gatccggacg
     2521 gtcatgaccg gcctgcgcga ggcgatcggg gcgggccttc ggacgaaggt cggcgggctg
     2581 ctcatccgcc cggccgcgcg cgagctccgc gcgaagctcg acccggacac gtacggcggc
     2641 gcctacctcc tcggcctcaa ggggctcgtc gtgatcgcgc acgggagctc gtcgagcgcg
     2701 gcggcggcga acgcgatccg gctcgccgca cgcggcgtcg agggacgggt cgtcgagcgg
     2761 gtcgccgcgc ggctcgccga ggggcgggcc gaagccgcgg ggtagcgcgg gaggagcatg
     2821 ttacgatcgg acgtccgaaa atccggggtc gcgaaaagga gtcgaggaga ccgcatggca
     2881 gcttccaggg aggagatcca cgagcaggtc aagaacgtgc tcgtcgagca gctcggcatc
     2941 gacgagggag caatcaccga ggacgcctcc ttccaggaag acctcgacgc cgactccctg
     3001 gatctcgtcg agctcatcat ggagctcgag gatcagttcg ggatcaagat ctccgacgag
     3061 gatgcgcagg ggatcacgac cgtcggacag gccgtcgact acatctcgaa gcatcagtag
     3121 cgccgccgct ccggacgcga caccgccgca cgccgagctc ctcgggctga tcgaggcatt
     3181 gcccgacggg cgcctccagc acgtcttcac gcacacgtcc tgggcgccga gccgcgagga
     3241 ctcctacgag cgcctcgagt tcctcgggga cagcgtgctc gggctcgcga tcgcccgcga
     3301 gctctacgag cgcttccccg agtaccccga gggacagctc gcgaagatcc gcgcccacgt
     3361 cgtgtcgcgc cagagctgcg ccgtcgtcgg gcggcggctc gacctcgggc agaagctgat
     3421 cgagcgggga gccgactccg tcccgcgcga ggagctcgag cggctctccg tcaaccggaa
     3481 cgtgctcgcc gccctcgtcg aggcggcgct cggcgcgctc tacctcgagc acgggttcga
     3541 gcggatccgc gaggcggtgc tcgccgcgtt cgccgaccgg atcgactacg cgctcacgac
     3601 gtacgtcgac tacaagaccg agctccagga ggagctcgcc cgccgctccc agagcgtctc
     3661 ctacaccgtc ctcgaggtcg acgggccgcc gcacgaccgc cgcttcacgt gcgcggccgt
     3721 catcgacggc gagcaggcgg ggagcgggac cggcgcgtcg aagaaggccg ccgagcagaa
     3781 cgccgcccgc gaggcgctgc gctccctgcc gccggtctag ccgagggcga cgagctcccg
     3841 gtggagcgcc tgggtgggcg gcgccggtgc gacgccgagg tcctcgcgca gccggaggcg
     3901 gaggcggtcg tagacctgga gcgcctcggc cgggttgccg cgccggacga gcgtccgcat
     3961 gagcgcccgg tggccgctct cccggtacgg cgcccgctcg acgaggcggc gcgcggcccg
     4021 ctcggcggtc gcgagctcgg atccgccgat cccgaggcag gcttcggcga cgagctcgag
     4081 cgcgcggaga tgccactcct cgagccgccg ccggcgctcg gcgatccacg gtgcgtcatc
     4141 gcccgagagg agctcgcgcg cggtcacgtg ctggacgacg cgcgccgggc cccacgcggc
     4201 cttccagtcc tcgcgcgcga cggccgactc ggcccgatgg agcgcctccg ccgccgcctc
     4261 gacgtcgacc cacgcgtccg cgggcagcgt gagcgcgacg ggcgtcccgc tcccgagccg
     4321 ggccgggccg agcgcgcggc ggagcttcga gaggagcgcc gagagcgccg cgtcggacgc
     4381 cgcaggcgcc ccatccggcc acacggcctc cacgagctcg ttccgcgaga ccgggcggtg
     4441 ccgctcgagg acgaggtacg cgaggagcac gcgtccctgc cgcccgggca gctccgcgtc
     4501 cagccgccgg tcgccgtcgc gcacgacgac gcgcccgcag agctcgatcc ggagaggccc
     4561 caaggtcacg ccaaggtaac gacaaggcgc agacgggaca atgcgctgca accgaccgag
     4621 ggaggctcct gtggcgaggg tgatcaactg cgagtgcggg cgcacgatcc gcggcgagac
     4681 ggacgaggag gtcgtggcga acgccgaggg gcacgtccgc gaggaccatc ccgagctcgt
     4741 cggctcgatc acgcgcgacg acctgctcgc gatgaccgag gaggcatagt ggcgcgactc
     4801 ctcgtgaacg tcgcgaccgg gccggagaac ccgacgcggg ccgcgctcgg cctgctcgtc
     4861 gcgcggacgg cggcggccga ggggcacgag gtgcgcgtct tcctcgccgg ggacggcgtc
     4921 cacctcgcgc gccccgagac ggcggcggcg acgcacggga tcggcaccgg ggcggccggc
     4981 gagcatctcg aggcgctccg cgcggccggc gtgccggtcc tcctctcggg catgtcgagc
     5041 aaggcgcgcg ggatcgaggg cggcggcgag ctttccccgc ccgagcggct cgtcgagctc
     5101 gcggcctggg ccgacacggc gctcgtgtac tgacgggcgg gcgtccgccg cgcagggcat
     5161 gcttcctgcc gcgtgaacct gcgctcgatc cgcatgcgcg gcttcaagtc gttccccgac
     5221 gaggtcgagg tgcgcctgga gcccggcgtc gccgtggtcg tcgggccgaa cggctccggg
     5281 aagtcgaaca tcgccgacgc ggtcgtctgg gcggccgggt cgctcagccc gtccgagctg
     5341 cgcgcggaga agcccgagga cgtcctcttc gccggctcgg cctctcgccc cggcgcggac
     5401 gcctgctcgg tcgagctcct cttcgacaac ccggacggcg acgagccgct tccctacgcc
     5461 gaggtctcgg tcgcgcgccg cctcgagcgc ggcggcgagg gccagtacct cgtgaacggc
     5521 gcgcacgtcc ggcgcaccga tctcgtggag ctcctcgccg atctcgggct cggcggcgag
     5581 atgcactcgg tcatctcgca ggggcgcgtg gagcagatcc tcgcctcgaa gccgtccgag
     5641 cggcgcgcgc tcgtcgagga ggcggccggg ctcgggcgct tcaagcgccg ccggcaccgc
     5701 gcggagctca agctcgcgcg tgtcgcgacg caggtcgagc gggcgctcga cctcgaggcc
     5761 gaggtgcgga agcggttgcg gccgctcgcg ctccaggcga ccgccgcgga gcgggcccag
     5821 aagctcgcgg gccagatcga cgagctcagc gtccggctcg ccgccgccga cctcgaccgg
     5881 ctcgaccgca cgctcggcga gatcgccggg cgccgtgccg ccgcggagcg ggagcggaag
     5941 cgccacgacg agcgcctctc cgcgctgctg gaggagcgcc gcgaggcgga ggaggagctc
     6001 ggggaggccg gcgggcgcca cgagcaggcg acggcggcgc tctaccggct ccggagcgcg
     6061 gtcgagcggg ccgagctgcg gcgcgagcgc gcgctcgaga ccgcgaagac cctccgcgcc
     6121 gagctcgacc ggccgcgcct gccgctcccc gaggcgccgc cgtcctacga gcaggtcgtg
     6181 cgagccgccc ggctctggcc cgcagtcccc gccgcggatc tcgacgccgg cgccgagctg
     6241 ctgcgccgcg cggccgacga gcagcgcgac gcgctcctcg tcgcctccgg cggcgagacg
     6301 tggttcgccg ccgacgccga ggcgaagcgc gacgcgccgg agaagcggcc gggcgtcccg
     6361 ctcgccgccc tcgccgacgc gtcggagcgg gcggcgacag ccctcgagcg ggcggcggcc
     6421 tcggcgggcc gcttcgagcc ggccctcgcg gcgcgtgtgg acgccggcgg ctcgcgctcg
     6481 ggagagctcg gcgagcggct gcgggcgctc ggggcgcagg aggcggatct ccgtcgcgcc
     6541 gccgcggagg cggccgagcg gctcacccag gtgcaggtcg aggcggcgcg gctcgagcgt
     6601 gagcgcgagg acgtcgtcga gcgcctcccc gaggggcacg aggccgaggg gctcacggac
     6661 gacgaggcgg ccgagcttcg cgcgcaggtc gagcggctcg agcgccgccg cgaggcgctc
     6721 ggccaggtga accccctcgc ggccgaggag cacgcgcgcg agaaggagcg cctcgacgac
     6781 ctcgtcgagc agcggcagga tctcgagcgc tccctcaccg agctcgagac gctccgcgcg
     6841 gagctcaccg agacggtcga gcgccgcttc gccgagacgt acgagcgggt cgagcaccac
     6901 ttcgccgagg tcgtcgcgac gctcttcccc ggcggcacgg ggcggctgat cgccgtcgac
     6961 cccgaggagg agggcgagga gcacggcgtc gagatcgagc tccagccggc cggcaagcgg
     7021 atcacgcggc tctcgctcct ctcgggcggc gagaaggcgc tcggcgcgat ctcgttcctc
     7081 ttcgcgctct tcctcgcgcg gccgtgcccg ttctacctgc tcgacgaggt cgaggcggcg
     7141 ctcgacgaca cgaacatcgg gcgcttcgtg gagctcctgc gccgcttctc cgaccgggcc
     7201 cagttcatcg tcgtcacgca ccagaagagg acgatggagg ccgcggatat cctctacggc
     7261 gtgacgatgg gcgccgacgg ggtctcgcag gtcgtgtcca agcggctcac ggccgagcgg
     7321 gctgccagcg cggccgcgtg agccgcagct gggccgagtt cctcggcgac ccggcgcccg
     7381 cgtccgaacc cgaaacgggt gggcgcgggg gctggttccg ccggctgcgc gactcgctcg
     7441 cgacgagccg cgaggcgctc acgagcgagc tcgcgtccgc ggtcttcgac ccggccgacg
     7501 acgagtcgtg ggagcggatc gaggaggcgc tcctgcgcgc ggacgtcggc gtgccggcga
     7561 cggtcgagat cgtgcgccgg ctcgaggagc ggcgcccggc gacgcaggcc gagctcgtcg
     7621 aggggctcga ggaggtgctg accgatctcg tctcaccggc cggcgaggcg cggctcgggc
     7681 tgggcgggcg gccgagcgtc gtcctcgtcg tcggcgtg
//
LOCUS       DASTFC010000356         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1115874,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000356 DASTFC010000000
VERSION     DASTFC010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115874"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFO88_10530"
     CDS             <1..1107
                     /locus_tag="VFO88_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9545999.1"
                     /translation="VSCAGLPANRLTAVTVPKNNPKAARIASENIVYPGSGGCHDTNV
                     FEEKQRILTACLGSGVSMLDISDPLNATTVWGPFTWPNHGTWHTGSWSWSGDYVYVNG
                     EPGGGSASECAFDDHPAKPTVFALNADTGTLEGLWTLPRPQETTSSTSNCTVHNLNVL
                     PLANKNVLAHSFYTAGASLIDFTNPKAPEEVAFIDQILTPGGGPVVPSGAGCWSAYWY
                     NGSIFCNELSWGLHIFDVNVPFWDDTMTLMSHNAQTVTNFIRCQVSFTGGPVRAGQTR
                     QVRATARLYGPAPLQAAKDTNVRISGPGFNRLVTTNDNGVAVATVRAARAGTLRVAVR
                     EADNLNITGNCSATRSIARKAATRTARSAQAVRR"
     gene            complement(1223..1308)
                     /locus_tag="VFO88_10535"
     tRNA            complement(1223..1308)
                     /locus_tag="VFO88_10535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:Leu,seq:gag)
     gene            1398..2036
                     /gene="lexA"
                     /locus_tag="VFO88_10540"
     CDS             1398..2036
                     /gene="lexA"
                     /locus_tag="VFO88_10540"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9546000.1"
                     /translation="MLTQRQREIYDFVTGYVDRHGYPPTVREIGEAVGLASPSTVHAH
                     LANLERAGYLRRDPTKPRALELVGRERAPAAEEVGEAGRVRVLPLVGQIAAGAPLLAD
                     ENVEQHIAVPEPLSSGVGEEFLLRVRGDSMIEAGILEDDYVVVRRQQTAANGDIVVAL
                     VGEDETADEATVKRFFREDGRVRLQPENSALEPLYPAHVQLLGKVTGVFRRV"
     gene            2038..2247
                     /locus_tag="VFO88_10545"
     CDS             2038..2247
                     /locus_tag="VFO88_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546001.1"
                     /translation="MTDVLQLNRTLEQELFALARGVSLECLVCGEFVLHAPDGSIRCP
                     ECESSLSRLVGAALAPGARAAEQGA"
     gene            complement(2252..>3309)
                     /locus_tag="VFO88_10550"
     CDS             complement(2252..>3309)
                     /locus_tag="VFO88_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9546002.1"
                     /translation="YWCAADIGWVTGHSYIVYGPLCNGTTSVLYEGTPDFPDRDRWWE
                     IVERYSVDVLYTAPTAIRAHMKWGTEHAAKHDLSSLRLLGSVGEPINPEAWMWYRENI
                     GGGRTPVVDTWWQTETGMILISPLPGVTTVKPGSATQPFPGIDAAVVDERGEEVPNGQ
                     GGYLVLRKPWPAMLRGLYKEHDRYVDTYWSRFPGLYFTGDGAKRDEDGDFWLMGRVDD
                     VMNVSGHRISTIEVESALVDHPAVAEAAVCGRKDDLTGEAIVAFVTLKGSEEVSVEML
                     DTLREHVAQKIGKIARPANIVFTPELPKTRSGKIMRRLLRDVSEHRTLGDTTTLADPS
                     VVEEIRDRAEDEKGKAD"
BASE COUNT          507 a         1177 c         1147 g          478 t
ORIGIN      
        1 gtgagctgcg ccggcctccc ggcgaaccgc ctcaccgcgg tgacggtgcc gaagaacaac
       61 ccgaaggcgg cccgcatcgc gagcgagaac atcgtctacc cgggctcggg cggctgccac
      121 gacacgaacg tgttcgagga gaagcagcgc atcctcacgg cgtgcctcgg ctccggcgtc
      181 tcgatgctcg acatcagcga cccgctgaac gccacgaccg tgtggggccc gttcacctgg
      241 ccgaaccacg gcacctggca caccggctcc tggagctgga gcggtgacta cgtgtacgtc
      301 aacggcgagc cgggcggcgg cagcgcctcc gagtgcgcct tcgacgacca tcccgcgaag
      361 ccgaccgtgt tcgcgctgaa cgcggacacc gggacgctcg agggcctgtg gacgctcccg
      421 cgcccacagg agacgacgag ctcgacgagc aactgcaccg tccacaacct gaacgtgctt
      481 ccgctcgcga acaagaacgt gctcgcgcac agcttctaca cggcgggcgc gtcgctcatc
      541 gacttcacca acccgaaggc gccggaggag gtcgcgttca tcgaccagat cctgacgccc
      601 ggcggtggcc cggtcgtccc gagcggcgca ggttgctggt cggcctactg gtacaacggc
      661 tcgatcttct gcaacgagct cagctggggc ctgcacatct tcgacgtcaa cgtgcccttc
      721 tgggatgaca cgatgacgct catgtcccac aacgcgcaga cggtgacgaa cttcatccgc
      781 tgccaggtgt ccttcacggg cggcccggtg cgggccggcc agacccggca ggtgcgggcg
      841 acggcgaggc tctacggccc ggccccgctc caggcggcga aggacacgaa cgtccgcatc
      901 agcgggcccg gcttcaaccg gctcgtcacg acgaacgaca acggcgtcgc agtcgccacg
      961 gtccgcgcgg ccagggccgg cacgctgcgt gtcgccgtcc gcgaggcgga caacctgaac
     1021 atcaccggca actgctcggc gacgaggtcg atcgccagga aggcggccac gaggacggcc
     1081 aggtccgccc aggcggtccg ccgctagaca aagggaagca acggcgcgat caggcgccga
     1141 gggaagcaga agggggcccg ccacgcgggc ccccttcgcg ttccgggaac gaaaaggggc
     1201 ggctctttcg agccgcccct catgcgctgg agaggacttg aacctccacg ggccatacgg
     1261 cccacaaggc cctcaacctt gcgcgtctac caattccgcc accagcgcgc ggaggtgaag
     1321 tctagcgtct ggttgacgcc gtccgatgcg gccggtagcc tgcgaacata cgttcgtcca
     1381 acggaggtgc cgagccgatg ctgacgcagc gacagcgcga gatctacgac ttcgtgacgg
     1441 ggtacgtcga ccggcacggc tacccgccga ccgtgcgtga gatcggagag gccgtgggcc
     1501 tcgcgtcgcc gtccacggtg cacgcgcacc tcgccaacct cgagcgtgcg ggctacctgc
     1561 gccgcgaccc gacgaagccg cgcgcgctcg agctcgtcgg ccgcgagcgg gcgccggccg
     1621 ccgaggaggt cggcgaggcg ggccgcgtgc gcgtcctgcc gctcgtcggc cagatcgccg
     1681 cgggcgcccc gctgctcgcc gacgagaacg tcgagcagca catcgccgtc ccggagccgc
     1741 tctcgtcagg cgtgggggag gagttcctgc tgcgggtccg cggtgactcg atgatcgagg
     1801 ccgggatcct cgaggacgac tacgtggtcg tccgccgcca gcagactgcc gcgaacggcg
     1861 acatcgtcgt cgcgctcgtc ggcgaggacg agacggccga cgaggcgacc gtgaagcgct
     1921 tcttccgtga ggacggccgg gtgcggctcc agccggagaa ctcggcgctc gagccgctct
     1981 accccgcgca cgtgcagctt ctcgggaagg tgacgggcgt cttccggagg gtatgaggtg
     2041 acggacgtgc tgcagctcaa ccggacactg gagcaggagc tcttcgcgct cgcgcggggc
     2101 gtcagcctcg agtgcctcgt ctgcggggag ttcgtcctcc acgcgccgga cggctcgatc
     2161 cgctgtccgg agtgcgagag cagcctctcg cggctcgtcg gcgccgcgct cgcgccgggc
     2221 gcgcgggcgg ccgagcaggg cgcgtagagg gctagtccgc ctttcccttc tcgtcctcgg
     2281 cgcggtcgcg gatctcctcg accacgctcg ggtcggcgag cgtcgtcgtg tcgccgagcg
     2341 tgcggtgctc ggagacgtca cgcaggaggc ggcgcatgat cttcccggag cgggtcttcg
     2401 gcagctcggg cgtgaagacg atgttcgcgg ggcgggcgat cttgccgatc ttctgcgcca
     2461 cgtgctcgcg cagcgtgtcg agcatctcga ccgagacctc ctcggagccc ttgagcgtca
     2521 cgaacgcgac gatcgcctcg cccgtcaggt cgtccttgcg cccgcagacc gccgcctcgg
     2581 cgaccgcggg atggtcgacg agcgcgcttt ccacctcgat cgtcgagatc cggtgccccg
     2641 agacgttcat cacgtcgtcc acgcggccca tgagccagaa gtcgccgtcc tcgtcgcgct
     2701 tcgcgccgtc gcccgtgaag tacaggccgg ggaagcggct ccagtacgtg tcgacgtagc
     2761 ggtcgtgctc cttgtagagg ccgcggagca tcgccggcca cggcttgcgg agcacgaggt
     2821 agccgccctg gccgttcggc acctcctcgc cgcgctcgtc cacgaccgcg gcgtcgatcc
     2881 ccgggaacgg ctgcgtcgcg gagcccggct tcacggtcgt cacgccgggg agcgggctga
     2941 tgaggatcat ccccgtctcc gtctgccacc acgtgtcgac gaccggcgtg cgtcccccgc
     3001 cgatgttctc gcggtaccac atccacgcct cggggttgat cggctcgccg accgacccga
     3061 gcaggcggag cgaggagaga tcgtgcttcg ccgcgtgctc cgtgccccac ttcatatgcg
     3121 cgcggatcgc ggtcggcgcg gtgtagagaa cgtccacgct gtagcgctcg acgatctccc
     3181 accagcggtc gcggtcgggg aagtccggcg tgccctcgta gagcaccgac gtcgtcccgt
     3241 tgcagagcgg cccgtagacg atgtagctgt ggccggtcac ccagccgatg tcggccgcgc
     3301 accagtaga
//
LOCUS       DASTFC010000357         3684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1379706,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000357 DASTFC010000000
VERSION     DASTFC010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379706"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1056)
                     /locus_tag="VFO88_10555"
     CDS             complement(100..1056)
                     /locus_tag="VFO88_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9546003.1"
                     /translation="MQRRFRSLLARRRLVIGLQVGFVVAFTAVLGYFLRDVWADALPL
                     LREADAVYVGLALRLLASYYLLFVLGWQWILAGLGIGIGYPLALQAEMASMLAKYVPG
                     TVWTPLARIVWLRRAGVRQTSVVVGSIALEAGLSALSGVLVFALSLILVGGQGPEVTV
                     PLAAFALVVGVLIHPRVFGPLARRIFRRFGGEAIPVLSYRLMLALLAFYSFTWLVGGV
                     AVYFLVRSLGGDPGLESIPFLGGTAAVGAIVAVLSVFAPSGLGVREGSMYGLMLAVTS
                     APVALGVTVLNRLAITIVEALLLAAGLVAWRLGRRDPAADTP"
     gene            complement(1066..3270)
                     /locus_tag="VFO88_10560"
     CDS             complement(1066..3270)
                     /locus_tag="VFO88_10560"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9546004.1"
                     /translation="MNAYARLVVALRYPIILAWIAGALAATAYLPDLSGEQGAPLEGL
                     VADDSPAVRTELRSAELFDVPVLTRTVVVRRDPEGLAPGAEQVALAGALAVSQGEDPD
                     LQRVRFALPVVNTLGVVPSSRERGTTVVTFLFFSPATFLAHQDELAHRYAERYAAGPE
                     AGFVGVTGAIPARLDQFDAIQAALPRVEQVTVLVIALILALTFRAPGAALVPLLSAGL
                     GYLVAVRALAAVGDAIGVNVPRELEPLVLVLMLGIVTDYAIFFLASFRRRLEEGAPRL
                     DAARETTISIVPIVLTAGLIVTFGTAALLAGTLDFFRAFGPGMALAAFVGVLLAVTFI
                     PAALAVFGRAVFWPSLRRRDPEAAREEERTRRPPRIARVTASRGGALAVALLCVAALL
                     AGATGLRDLRLGFTLITGLPKDAEAHRAAREASEGFAPGLLAPTTVLVEAPGIASRPG
                     AIRRFDRALERQSGVVASIGPTDAPRALAGNALVAGSGDAVRYLLVFDDDPGGGPAIE
                     SLERIRDSMPLLQEASGLSEARVGLAGDTALAADTVGRIVDDLARIAVAALLANLFFL
                     VVFLRALVAPLFLLAASVLALAASLGLATYVFQGLLGHGELTYYVPFAAAVLLVSLGS
                     DYNIFVAGRVWAEARRRPLREAVAVAAPRASRAIAIASIALAASFAVLALVPLRPFRE
                     FAFIMAAGVLVDSFVVRSLLVPALISLVGDASAWPGRFTLPRTVEPDARDAG"
     gene            complement(3441..>3684)
                     /gene="heR"
                     /locus_tag="VFO88_10565"
     CDS             complement(3441..>3684)
                     /gene="heR"
                     /locus_tag="VFO88_10565"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HET9546005.1"
                     /translation="AIGLQLGYSEAETGDVPTFVYAIFFSLFVLFFSFAVNMALQYGR
                     VGPWRSYLFGERGYLVLSLVAKSALAWQVFGSTLAD"
BASE COUNT          644 a         1281 c         1391 g          368 t
ORIGIN      
        1 cgcgaagatc cgcctgctgc ggtcgaggcc gcgtatctcg ccggtcagga agcccttcca
       61 ctcggcctcc gtcggctgtt ccttcgacat gctccgagtc tagggcgtgt cggcggccgg
      121 atcgcggcgt ccgagccgcc atgcgacgag gccggcggcg aggagcagcg cctcgacgat
      181 cgtgatcgcc aggcggttca gcacggtgac cccgagcgcg accggcgcgg acgtgacggc
      241 gagcatcagc ccgtacatcg acccctcgcg caccccgagc cccgacggtg cgaagacgga
      301 gaggacggcc acgatcgcgc cgaccgcggc cgtccccccg aggaacggga tcgactcgag
      361 ccccggatcc ccgccgagcg agcgcacgag gaagtagacg gcgacgccgc cgacgagcca
      421 tgtgaacgag tagaacgcga gcagcgccag catgagccgg taggagagaa cggggatcgc
      481 ctctccgccg aagcggcgga agatgcgccg cgcgagcggg ccgaacacgc gcggatggat
      541 gagcaccccg acgacgagcg cgaacgccgc gagcggcacg gtcacctccg gtccctgccc
      601 gccgaccagg atcaggctga gcgcgaagac gagcaccccc gagagcgccg agagcccggc
      661 ctcgagcgcg atcgagccaa ccacgaccga cgtctgcctg acacccgccc ggcgaagcca
      721 gacgatccgg gcgagcgggg tccagaccgt cccgggcacg tacttcgcga gcatcgaggc
      781 catctccgcc tggagcgcga gcgggtagcc gatgccgatc ccgagcccgg cgaggatcca
      841 ctgccacccg agcacgaaga ggaggtagta ggaggcgagg aggcggagcg cgagcccgac
      901 gtagacggcg tccgcctcac gcaggagcgg gagcgcgtcc gcccagacgt cccgcaggaa
      961 gtagccgagg acggccgtga acgcgacgac gaacccgacc tggagtccga tgacgaggcg
     1021 gcggcgcgcg aggagggagc ggaaacgccg ctgcacgcga cgactctagc ccgcgtcccg
     1081 ggcgtcgggc tcgaccgtcc gcgggagcgt gaagcggccc ggccatgcgc tcgcgtcgcc
     1141 gacgagcgag atgagcgcgg ggacgaggag cgagcgcacg acgaaggagt cgacgagcac
     1201 gcccgccgcc atgatgaagg cgaactcccg gaaggggcgt aacggcacga gcgcgagcac
     1261 ggcgaagctc gccgcgaggg cgatcgacgc gatcgcaatc gccctcgaag cgcgcggcgc
     1321 ggccactgcg accgcctccc ggagagggcg ccggcgcgcc tccgcccaga cgcgcccggc
     1381 gacgaagatg ttgtagtcgg agccgagcga gacgagcagg acggctgcgg cgaacggcac
     1441 gtagtacgtg agttctccgt ggccgaggag tccctggaac acgtatgtgg cgagccccag
     1501 cgaggcggcg agcgcgagta cgctcgccgc gagcaggaag agcggcgcga cgagcgcgcg
     1561 gaggaagacg acgagaaaga agaggttcgc caggagcgcg gcgacggcga tccgtgcgag
     1621 atcgtcgacg atccgcccga ccgtatcggc ggcgagtgcc gtatcgcccg cgagcccgac
     1681 gcgcgcctcg gagagcccgc tcgcctcctg caggagcggc atcgagtccc ggatgcgctc
     1741 gagcgactcg atcgccggcc cgcccccggg gtcatcgtcg aagacgagca ggtagcggac
     1801 ggcatcgccg ctccctgcga cgagcgcgtt cccggccagg gcgcgcgggg catcggtcgg
     1861 gccgatcgaa gcgacgacgc cggactgtcg ctcgagggcg cggtcgaagc gccggatcgc
     1921 tcccggccgg cttgcgatgc cgggtgcctc gacgagaacg gtcgtgggcg cgagcaggcc
     1981 gggcgcgaag ccctcgctcg cctcccgcgc cgcccggtgg gcctcggcat ccttgggcag
     2041 cccggtgatg agcgtgaagc ccagccggag gtcacggagc cccgtggcgc cggccaggag
     2101 cgccgcgacg cagaggaggg cgacggcgag cgcgccgccc cgcgatgccg tcacgcgtgc
     2161 gatgcgtgga ggacgccgcg tccgctcctc ctcgcgcgcg gcctcgggat cgcgccggcg
     2221 aagcgagggc cagaagaccg cccgtccgaa gacggcgagc gccgcaggga tgaacgtcac
     2281 ggcgagaaga acgccgacga acgcggcaag cgccatgccg ggaccgaagg cgcggaagaa
     2341 gtcgagcgtc ccggcgagga gcgcggcggt tccgaacgtg acgatgagcc cggcggtcag
     2401 cacgatcggg acgatcgaga tcgtagtctc ccgcgccgca tcgagccgag gagcgccctc
     2461 ctcgaggcgc ctccgaaacg aggcgagaaa gaagatcgcg tagtccgtga cgatcccgag
     2521 catcagcacg agcacgagcg gctcgagctc gcgcggtacg ttgaccccga tcgcgtcccc
     2581 gaccgcggcg agcgcgcgga cggcgacgag atagccgagc ccggccgaga ggagggggac
     2641 gagcgccgcg ccgggtgcgc ggaacgtgag cgcgaggatg agcgcgatca cgagtacggt
     2701 cacctgctcc acgcgcggga gcgccgcctg gatcgcgtcg aactggtcga gccgggccgg
     2761 gatcgcgccg gtcacgccga cgaagccggc ctcgggccct gccgcgtagc gttcggcgta
     2821 ccggtgcgca agctcgtcct ggtgggcgag aaacgtcgcg ggcgagaaga agaggaacgt
     2881 gacgaccgtc gtcccgcgct cgcgcgagga cggcacgacg ccgagcgtgt tcacgaccgg
     2941 gagcgcgaac cgcacgcgct gcagatcggg gtcctcaccc tggcttaccg cgagcgcgcc
     3001 ggcgagcgcg acctgctcgg cgccgggcgc gagcccctcc ggatcacgcc ggacgacgac
     3061 ggtgcgggtg aggaccggga cgtcgaagag ctccgccgag cgcagctccg tccggacggc
     3121 gggcgagtcg tctgcgacga gaccctcgag cggcgccccc tgctcgcccg acaggtccgg
     3181 gaggtaggcg gtcgccgcga gcgcgcccgc tatccaggcg aggatgatcg ggtaacgcag
     3241 cgcgacgacg agccgcgcgt aggcgttcat cccggcggct ccggcccctg cggcgcgacg
     3301 ctcgagcgtc cgagctcgac gtccgcaagg cgttgccggc gccacacgtc accgccgcgc
     3361 acgacgctgc cgcgggcgtg gacgtcgcga gcgagccgtc tcttcatgcc ggacgagttc
     3421 ccgctcgccc gcattcctag ttagtcggcg agcgtcgagc cgaagacctg ccaggcgagc
     3481 gcgctcttcg cgacgaggct gaggacgagg tagccgcgct ccccgaagag gtagcttcgc
     3541 cacggcccga cgcgcccgta ctggagcgcc atgttgaccg cgaagctgaa gaagaggacg
     3601 aagagggaga agaagatcgc gtacacgaag gtcggcacgt cgccggtctc cgcctccgag
     3661 tacccgagct ggagcccgat cgcg
//
LOCUS       DASTFC010000358         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_473364,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000358 DASTFC010000000
VERSION     DASTFC010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473364"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VFO88_10570"
     CDS             complement(<1..1363)
                     /locus_tag="VFO88_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HET9546006.1"
                     /translation="MITADSPPAAPSERRLMLGAAALGLVLTGLAVAAVVWSGQPGDH
                     TVIALASGLAVAIPVAVGLTVWRAGEYVRFGKLLVAWGLIDFVPVLSYSSDPTAYSVG
                     RIGVWLVQPISIAAMLAFPSGRLRSRADRGIVLLMVATVAFLYLPTALLVDRYPEPSL
                     WTTCSESCPDNAFQVVDPEPAFVEALIQPLREGITALAHILVVIVLARRLHASSEVAR
                     RLVAPVLGVAIARSAVMAVGLPIRDVAPDATYVLSWVFVLSLPAIAVAFAVGIVRSRL
                     VAGDALAALARRLRREPGAGDVRDVLAETLDDPTLEVAYWLPDGPGRWIDAAGTDVRL
                     PTDESRSVTEVREDGRRVAALVHDPVLREQGSFVEAAGAFALSALENQRLRAQVDASL
                     DELRESRARIQAAADSERRRIERDLHDGAQQRLVALRVRLELAAETVRDDPDESARVL
                     RQLG"
     gene            complement(1421..1846)
                     /locus_tag="VFO88_10575"
     CDS             complement(1421..1846)
                     /locus_tag="VFO88_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9546007.1"
                     /translation="MRSDTVHLAPGRPQDAGDVTVLTVDDQAAFRGAARAVIEATPGF
                     RPVGEAASGEEALRLLDDVAPGLVLLDVRMPGLDGVETARRIAARDVHPVVVLVTAED
                     AGALPPDVSRCGAAAVARKQDFGRRLLTELWAEHGDTAR"
     gene            1991..2671
                     /locus_tag="VFO88_10580"
     CDS             1991..2671
                     /locus_tag="VFO88_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9546008.1"
                     /translation="MSVRVAVADDDFLVREGLLQVLERAPGVEVVADCEDLPALTEAV
                     ARLDPDVALTDIRMPPTKTDEGIRFAVEARASNPRLAVIVLSQYVEPSYALRLLEEGT
                     ERRGYLLKERVHDRAQLVRAIETVAAGGAVVDPAVVDELVRAKRREDESPLERLTPRE
                     QEVLAEIAQGKSNAAVAESLVLTKRAVEKHINSIFLKLGLADTADVSKRVKAALLFLG
                     EPGTGHSA"
     gene            2876..>3063
                     /locus_tag="VFO88_10585"
     CDS             2876..>3063
                     /locus_tag="VFO88_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546009.1"
                     /translation="MTRQRSGGRAVAPDVAGFEAHHDAPRTSKEILARDPLTRRLLES
                     RDLLVMLTAPPGFGKTTLL"
BASE COUNT          447 a         1100 c         1157 g          359 t
ORIGIN      
        1 gaccgagctg ccggagcacc cgggcgctct cgtccgggtc gtcgcggacc gtctcggcgg
       61 cgagctcgag ccggacccgg agcgcgacga gccgctgctg cgcgccgtcg tggaggtcac
      121 gctcgatccg acgccgctcg ctgtccgccg cggcctggat ccgtgcgcgc gactcacgca
      181 gctcatcgag cgacgcgtcg acctgcgccc ggagccgctg gttctcgagg gcggagagcg
      241 cgaacgcgcc ggccgcctcg acgaaggatc cttgctcgcg caggaccggg tcgtgcacga
      301 gcgccgcgac gcgccggccg tcctcgcgca cctccgtgac cgaccgcgac tcgtcggtcg
      361 gaaggcggac gtccgtgccg gcggcgtcga tccaccgtcc gggcccgtcg ggcagccagt
      421 aggcgacctc gagcgtcggg tcgtcgagcg tctcggccag cacgtcgcgg acgtcgcccg
      481 cgcccggctc gcgccgcagc cgtcgggcga gagccgcgag cgcgtcgccc gcgaccagcc
      541 tcgaccgcac gatcccgacg gcgaacgcca ccgcgatcgc aggcagggaa aggacgaaga
      601 cccacgacag gacgtaggtg gcgtcgggcg ccacgtcccg gatcggcagg ccgaccgcca
      661 tcacggcgga gcgggcgatc gcgaccccga gcaccggcgc gacgagccgg cgcgcgacct
      721 ccgacgacgc atggaggcgc cgggcgagga cgatgacgac gaggatgtgc gccagcgccg
      781 tgatcccctc gcgcagcggc tggatcagcg cctcgacgaa ggcgggctcg gggtcgacga
      841 cctggaaggc gttgtcgggg cagctctccg agcaggtcgt ccagagcgac ggctcgggat
      901 agcggtcgac gagcagcgcc gtcgggagat acaggaacgc cacggttgcg accatgagga
      961 gcacgattcc gcggtccgcc cgcgagcgga ggcgacccga gggaaacgcg agcatcgcgg
     1021 cgatcgagat gggctggacg agccagacgc cgatccgccc gacgctgtag gcggtgggat
     1081 cgctcgagta ggagagcacc gggacgaagt cgatgaggcc ccaggcgacg aggagcttcc
     1141 cgaagcggac gtactcgccc gcacgccaga cggtgagccc gaccgcgacc ggaatggcga
     1201 ccgcgagccc gctcgcgagc gcgatcacgg tgtggtcgcc cggctggccg ctccagacga
     1261 ccgccgcgac ggcgagcccg gtgagcacga ggccgagcgc ggcggcgccg agcatgagcc
     1321 ggcgctcgct cggcgctgcg gggggtgagt ccgccgtaat catcgaccga agtgggacac
     1381 gcggggagga acgttactcc cgaagcccgc gggcgggcga tcagcgcgcg gtgtctccgt
     1441 gctccgccca gagctccgtg agcagccgtc ggccgaagtc ctgcttgcgc gcgacggcgg
     1501 cagcgccgca gcggctcacg tcgggcggga gcgcacccgc gtcctcggcg gtgacgagga
     1561 cgacgacggg gtgcacgtcg cgagccgcga tgcgccgggc ggtctcgacg ccgtcgagcc
     1621 ccggcatccg gacgtcgagg agcacgaggc ccggcgcgac gtcgtcgagg agacgcagcg
     1681 cctcctcgcc cgacgcggcc tctccgacgg gccggaagcc gggcgtcgcc tcgatgaccg
     1741 cccgcgcggc accccggaag gcggcctggt cgtcgacggt gaggaccgtg acgtcccccg
     1801 cgtcctgcgg acggccgggt gcgaggtgga cggtgtcaga gcgcatctct ccgagttcag
     1861 gatctgcgtc cgcgcgcggg gccgcaagac tgcgcgcagg ccggcgccca cctcgtgcgc
     1921 ctgtatcccg ggagggatgc ctgcacccct ttcccgccgg gtcgccgcgt gtcccgacta
     1981 tcgttccgcc gtgtccgttc gggtcgccgt ggccgatgac gacttcctcg tccgcgaggg
     2041 cctcttgcag gttctcgagc gcgcgcccgg ggtcgaggtc gtcgcggact gcgaggacct
     2101 tccggcgctc acggaggctg tggcgcggct cgaccccgac gtcgccctga cggacatccg
     2161 gatgccgccc acgaagaccg acgaggggat ccggttcgcc gtcgaggcgc gcgcgtcgaa
     2221 cccgaggctc gcggtcatcg tcctcagcca gtacgtcgag ccgtcctacg cgctccggct
     2281 gctcgaggag ggcacggagc ggcgcggcta tctcctgaag gagcgcgtcc acgaccgcgc
     2341 gcagctcgtc cgggcgatcg agacggtggc ggccggcggc gcggtcgtcg acccggccgt
     2401 cgtcgacgag ctcgtgcgcg caaagcgacg cgaggacgag tcgccgctcg agcgcctcac
     2461 gccgcgcgag caggaggtgc tggccgagat cgcgcagggc aagagcaacg ccgcggtcgc
     2521 cgagtcgctc gtcctgacga agcgagcggt cgagaagcac atcaactcga tcttcctgaa
     2581 gctcgggctc gccgacacgg ccgacgtgag caagcgcgtc aaggcggccc tgctcttcct
     2641 cggcgagccg ggcacgggcc actcggcgta gcggacgtcg gtcaccggcg cggatcgcct
     2701 ccggcccgcc ttccccgcac ggaaagggag acgagcgggc gccggggtgc gcgcaccacc
     2761 gccgggtcgc ctgccggccc cattgtggag gccggcgaag tggtgttcac tgtcggcggt
     2821 gagggtgcct aggcaccgag tctgggcgca gatgcccacg acgatcgggg agcccgtgac
     2881 gagacagcga tcggggggaa gagcggtcgc accggacgtc gccggcttcg aggcgcacca
     2941 cgacgcgccg aggacgtcga aggagatcct cgcccgtgac ccgctcacgc gccggctgct
     3001 cgagtctcgc gacctgctcg tcatgctcac ggcgccgccc ggcttcggca agacgacgct
     3061 cct
//
LOCUS       DASTFC010000359         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_721052,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000359 DASTFC010000000
VERSION     DASTFC010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721052"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFO88_10590"
     CDS             <1..372
                     /locus_tag="VFO88_10590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9546010.1"
                     /translation="LVRVRTPESFRERAAGKVPGATVAEVDGAIAGFVVVVDDEVEQV
                     FVAADHRGSGIAAQLLAAAEEQLRASGHETAWLAVVPGNARARAFYEREGWHDDGALE
                     YEAAGPDGPIAVPTRRYVKRL"
     gene            434..868
                     /locus_tag="VFO88_10595"
     CDS             434..868
                     /locus_tag="VFO88_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9546011.1"
                     /translation="MSIVDVMRSRLVAVAPDATGRGAIRRMAEADVGSVVVCDGSRLV
                     GIFTERDVLRLAGEGVDLDATRVADVMTRDVATVTPDTELLAAARLMGERRIRHLPVV
                     EGENVLGVVGIRDILGVLTERLWRTPDDDTRETVHELLSRRS"
     gene            complement(875..1618)
                     /locus_tag="VFO88_10600"
     CDS             complement(875..1618)
                     /locus_tag="VFO88_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET9546012.1"
                     /translation="MSALTVEGLHKSFGRLEVLRGIDLEVAEHEVVCLIGASGSGKST
                     FLRCVNLLEPIDAGTIVVAGEEITARRVDVNRVRRRIGIVFQAFNLFPHMSVLANVTL
                     APRRALRLSRADAEERAVELLERFGLADKRDEYPDRLSGGQQQRVAIVRALAMRPDLM
                     LLDEVTSALDPELVAEVLNVIRELAAAGMTMVIATHEMAFARDIADRVCFLDEGVILE
                     EGTPEAIFTRPREPRTQQFLQRIVEAGRL"
     gene            complement(1615..2469)
                     /locus_tag="VFO88_10605"
     CDS             complement(1615..2469)
                     /locus_tag="VFO88_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET9546013.1"
                     /translation="MPEPAQLGSRRSRILGGPRSEGARSTLIAVVSTVVFFGLVVLLV
                     TNAPGWPEVKRSFFSGEQFRESLPDIASAFGLNIRLFLVAEVLILVFALLLAVLRSVP
                     GPVFFPLRLLATAYTDLFRSIPTVLVIYILGFGAPTLQISGLPTSPFFWGVTALVLVY
                     SAYVAEVYRAGIQSVHPSQEAAARSLGLSQPQALRFVILPQAVRRVVPPLLNDFIGLQ
                     KDTALVALIGPVEAFRASQIEVAGSFNYTPYLATALLFILLTVPMARFTDWLIARNRR
                     RELAAGGR"
     gene            complement(2488..3414)
                     /locus_tag="VFO88_10610"
     CDS             complement(2488..3414)
                     /locus_tag="VFO88_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9546014.1"
                     /translation="MRKPILIVLVALLAALAAGCGGDDDADDVTTGAADTPAETDTTE
                     TVAEDTCAKESLNLVNPGQLTIGTDNPAFPPWFEGGTPEGSEWEINDPSTGEGFESAV
                     AYAVAEELGFTEDEVEWTVVPFNQSFRPGPKDFDFDINQISFTERRAQNVSFSDSYYD
                     VNQAVVALNSSPIADATSIADLADAKLGAPVGTTSYDYIVENVQPSQDPSVYDTLNDG
                     VSALAAGQIDGLVVDLPTAFFITAVQVEDGTIVGQFPSVGEQEYFAMAFELDNPLVDC
                     VNEALAALEEDGTLDGIQQEWLADKADAPVLE"
     gene            complement(3454..4497)
                     /locus_tag="VFO88_10615"
     CDS             complement(3454..4497)
                     /locus_tag="VFO88_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9546015.1"
                     /translation="MPEKVEVPAQADGVFEGGGVKGIAFAGAIKAAEEEGIREWVNVA
                     GSSAGAITASLLAVGYTADEIERALAETNYRLFADYGPGGKYLGGPLNYLRLRGIVPG
                     RYFKQWLGERFEAKLGNPNPTFKDVIRKDLPDNLSEAELRRAKYKLKVIASDITSGRM
                     LVLPDHIADYEDKDGNRFTVDEFPLVEAVRMSMSFPFFFDVVTLYRDGKPHYIVDGGL
                     LSNFPVWLFDSGDRPPRRPTWGFRLHGGTGPERLPYRPIRRPFWARPLLKAMFFSAME
                     AWDREQLGRTTAARTVSIPTRDVKTINFDLPKEQADSLYRWGYEETKEFFASPETQAY
                     LAKIQAARQETPA"
BASE COUNT          679 a         1714 c         1692 g          689 t
ORIGIN      
        1 ctcgtccgcg tccgcacgcc ggagtcgttc cgcgagcgcg cggccggcaa ggtgcccggc
       61 gcgaccgtcg cggaggtgga cggcgcgatc gccgggttcg tcgtcgtcgt ggacgacgag
      121 gtggagcagg tcttcgtcgc cgccgaccac cggggcagcg ggatcgccgc gcagctcctc
      181 gccgccgcgg aggagcagct ccgagcgagc ggccacgaga cggcgtggct cgcggtcgtc
      241 ccgggcaacg cccgggcccg cgccttctac gagcgtgagg gctggcacga cgacggcgcc
      301 ctcgagtacg aggccgccgg ccccgacggg ccgatcgccg tcccgacgcg ccgctacgtc
      361 aagcgcctct gagggcgggc cgagccactt ccttgctcac gtgcggtccg caacgagtaa
      421 attaagctgc ggagtgtcga tcgtcgacgt catgcgaagc cgcctcgtgg ccgtcgcacc
      481 ggacgcgacg gggcgcggcg cgatccgccg gatggccgag gccgacgtcg gctccgtcgt
      541 cgtgtgcgac gggtcccggc tcgtcgggat cttcaccgag cgggacgtcc tgcgcctcgc
      601 cggcgagggc gtcgacctcg acgcgacccg ggtcgccgac gtgatgaccc gcgacgtggc
      661 gaccgtcacg cccgacaccg agctgctcgc cgccgcgcgc ctcatgggcg agcgccggat
      721 ccgccatctg cccgtggtcg agggcgagaa cgtcctcggc gtcgtcggga tccgcgacat
      781 cctcggcgtc ctcacggagc gcctctggcg gacgcccgac gacgacacgc gcgagaccgt
      841 gcacgagctg ctctcgcgcc gctcgtagat ccggtcagag ccgccccgcc tccacgatcc
      901 gctgcaggaa ctgctgcgtg cgcggttcgc gcgggcgcgt gaagatcgcc tccggcgtgc
      961 cctcctcgag gatcacgccc tcgtcgagga agcagacccg gtcggcgatg tcgcgggcga
     1021 aggccatctc gtgggtcgcg atcaccatcg tcatcccggc cgcggcgagc tcgcggatga
     1081 cgttcaggac ctcggcgacg agctccgggt cgagcgcgct cgtcacctcg tcgaggagca
     1141 tgaggtcggg ccgcatggcg agcgcgcgca cgattgcgac gcgctgctgc tggccgcccg
     1201 agagccggtc cgggtactcg tcgcgcttgt ccgcgaggcc gaagcgctcg agcagctcga
     1261 ccgcccgctc ctccgcgtcc gcgcgcgaga gcctgagcgc ccggcgcggc gcgagcgtca
     1321 cgttcgcgag gacgctcatg tgcgggaaga ggttgaacgc ctggaagacg atcccgatcc
     1381 ggcggcgcac gcggttcacg tccaccctgc gcgcggtgat ctcctcgccc gcgacgacga
     1441 tcgtcccggc gtcgattggc tcgagcaggt tcacgcagcg gaggaacgtc gacttccccg
     1501 acccggacgc gccgatcagg cacacgacct cgtgctcggc gacctcgagg tcgatcccgc
     1561 gcagcacctc gagcctgccg aacgacttgt ggagcccctc gaccgtgagc gcgctcatcg
     1621 tccgcccgcc gcgagctcgc gccgccggtt gcgcgcgatc agccagtcgg tgaaccgcgc
     1681 catcgggacg gtcaggagga tgaagaggag cgccgtcgcg aggtacggcg tgtagttgaa
     1741 gctccccgcg acctcgatct gcgaggcgcg gaacgcctcc accggcccga tgagcgcgac
     1801 gagcgccgtg tccttttgga gcccgatgaa gtcgttcagg agcggcggca ccacgcgccg
     1861 caccgcctgc gggaggatca cgaagcgcag cgcctgcggc tgcgagagcc cgagcgagcg
     1921 cgcggccgcc tcctggctcg ggtgcaccga ctggatcccg gcgcggtaca cctccgccac
     1981 gtacgccgag tagacgagca cgagcgccgt cacgccccag aagaacggcg acgtcggcag
     2041 ccccgagatc tgcagcgtcg gcgcgccgaa cccgaggatg tagatcacga gcaccgtcgg
     2101 gatcgagcgg aagaggtcgg tgtacgccgt cgcgagcagc cggagcggga agaagaccgg
     2161 cccgggcacg ctccgcagga cggcgaggag gagcgcgaag acgaggatca gcacctccgc
     2221 gacgaggaag aggcggatgt tcagcccgaa cgcgctcgcg atgtccggga gcgactcgcg
     2281 gaactgctcg ccggagaaga acgaccgctt gacctccggc caccccggcg cgttcgtcac
     2341 gaggaggacg acgagcccga agaagacgac cgtcgagacg accgcgatca gggtgctgcg
     2401 ggcgccctcc gaccgcggcc cgccgaggat gcgggagcgg cggctgccga gctgggccgg
     2461 ctcgggcatc gggctacgcg cgggcggcta ctcgaggacc ggcgcgtcgg ccttgtccgc
     2521 cagccactcc tgctggatcc cgtccagcgt gccgtcctcc tcgagcgcgg cgagcgcctc
     2581 gttcacgcag tcgacgagcg ggttgtcgag ctcgaacgcc atggcgaagt actcctgctc
     2641 gccgacgctc gggaactggc cgacgatcgt cccgtcctcg acctggacgg ccgtgatgaa
     2701 gaacgcggtc gggaggtcga cgacgaggcc gtcgatctgg cccgcggcga gcgcggacac
     2761 gccgtcgttc agggtgtcgt agacggacgg gtcctggctc ggctggacgt tctcgacgat
     2821 gtagtcgtag ctcgtcgtgc cgaccggggc gccgagcttc gcgtcggcga ggtcggcgat
     2881 cgacgtggcg tcggcgatcg gcgaggagtt gagcgcgacg acggcctggt tcacgtcgta
     2941 gtacgagtcg ctgaagctga cgttctgcgc gcgccgctcg gtgaaggaga tctggttgat
     3001 gtcgaagtcg aagtccttcg ggcccgggcg gaaggactgg ttgaacggga cgaccgtcca
     3061 ctcgacctcg tcctccgtga acccgagctc ctcggccacc gcataggcga cggcgctctc
     3121 gaagccctcg cccgtggacg ggtcgttgat ctcccactcg gagccctcgg gcgtgccgcc
     3181 ctcgaaccac ggcgggaagg ccgggttgtc cgtcccgatc gtgagctgcc ccgggttgac
     3241 gaggttcagg ctctcctttg cgcacgtgtc ctcggcgacc gtctcggtcg tgtccgtctc
     3301 ggccggcgtg tcggccgcgc cggtcgtgac gtcgtcggca tcgtcgtcgc cgccgcagcc
     3361 cgcggcgagc gcggcgagca gcgcgacgag gacgatcagg atcggcttcc gcatccgagg
     3421 cagccgaatg taccacctcc ggcaagggac gagctacgcc ggcgtctcct gcctcgcggc
     3481 ctggatcttg gcgaggtacg cctgcgtctc ggggctcgcg aagaactcct tcgtctcctc
     3541 gtagccccag cggtacaggc tgtcggcctg ctccttcggc aggtcgaagt tgatcgtctt
     3601 cacgtcgcgc gtcgggatgc tcaccgtgcg cgccgcggtc gtccgcccga gctgctcgcg
     3661 gtcccacgcc tccatcgccg agaagaacat cgccttgagg agcggccgtg cccagaacgg
     3721 ccgcctgatc ggccggtacg gcagccgctc gggccccgtg ccgccgtgca ggcggaagcc
     3781 ccacgtcggc cgccgcggcg gccggtcgcc gctgtcgaag agccacacgg ggaagttcga
     3841 gagcaggccg ccgtcgacga tgtagtgcgg cttgccgtcc cggtagagcg tcacgacgtc
     3901 gaagaagaac gggaagctca tgctcatccg gacggcctcc acgagcggga actcgtcgac
     3961 ggtgaaccgg ttcccgtcct tgtcctcgta gtcggcgatg tggtcgggga ggacgagcat
     4021 ccggccgctc gtgatgtcgg acgcgatcac cttcagcttg tacttcgccc gcctgagctc
     4081 ggcctcggac aggttgtccg gcaggtcctt tcggatcacg tccttgaagg tcggattcgg
     4141 gttgccgagc ttcgcctcga agcgctcgcc gagccactgc ttgaagtacc tgcccgggac
     4201 gatgccgcgc agccgcaggt agttcagcgg cccgcccagg tacttcccgc ccggcccgta
     4261 gtccgcgaag agccggtagt tcgtctccgc gagggcccgc tcgatctcgt cggccgtgta
     4321 gccgaccgcg aggagcgagg cggtgatcgc gcccgcggac gagcccgcga cgttgaccca
     4381 ctcgcggatc ccctcctcct cggccgcctt gatcgccccc gcgaacgcga tccccttgac
     4441 gccgccgccc tcaaagacgc cgtccgcctg ggcgggaacc tcgaccttct ccggcatgac
     4501 ccctcctctc cgcgctgctc cgggtcggcg ggaatctaca agggatcccg gaccccgggg
     4561 agcgcccgct ccggcccgcg gcgggtcgct aggctcggcg catgtcgctt ccgcaggccg
     4621 agacgccgag cgcgcgcgtc aagccgcgcc tgcgcgggct ctcgcaccag atcgccttcg
     4681 tcgccgcgct tccgctcgcc gtcgcgctcg tcctcgacgc gaacaccaca cgcggacgag
     4741 tttcggccgc cgtcttcggc gcgagcgtcg cggc
//
LOCUS       DASTFC010000360         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_209920,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000360 DASTFC010000000
VERSION     DASTFC010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209920"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..2036)
                     /locus_tag="VFO88_10620"
     CDS             complement(105..2036)
                     /locus_tag="VFO88_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET9546016.1"
                     /translation="MSRANAASGNGIPNGNGAAPAVAEPPRPPQPPLTPMTRYAPRRR
                     SPLRVVGRVFAWLLVTLLVTVAGVAGGFWLWGEELSASTRPQTPEEREAQEVLDQVPP
                     ADQPAVALVVGYDFRHGEFEREESRSDTLMLLRADPRQKTISMLSFPRDLLVELPGCE
                     GAPPRMAKINEAFTDCGIKGTIETVRKLTGVPINYYVTIDFNGFIDTVNQLGGVYMDI
                     DRRYFNDNSQGGASYSAIDIRPGYQLLDGRDALAFVRYRHTDNDFYRNARQQEFVKSI
                     KHQVSGLSAAWRLPGIVNTVAKHVTIGSGGGKELSLDTIISYARLAYELPSGNLFQPR
                     LENLVDNEFFQLSAPEGEIERAVDEFMNPDPDAAAKATSVAVGGKTPANRRTGPPPSQ
                     VTVTVLNGNGVAGAADDAAVALAGRGYQAVNGGNADRLDYFETQVTYDPAVRGSEGAA
                     NQMARLFGDAELVPATPDAPLDTTLRVIVGRTFHGNLAPAPRDTTPEAAPPVVVTDPE
                     HAVAQAREAQRKVDFPVLVPTIRESASYLSTLQPMRTYRIRGEGAVRFVYSGPTELDY
                     WGIQQTSFTEAPALEGATLTRTISGRDYRLYFNGPKLHMVAFEENGAAYWVVNTLLDQ
                     LSNETMLEIAKGLKPLNAN"
     gene            complement(2119..>3387)
                     /locus_tag="VFO88_10625"
     CDS             complement(2119..>3387)
                     /locus_tag="VFO88_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HET9546017.1"
                     /translation="AEAEGADAVGLSGLITPSLDEMVHVAEEMERRGLDLPLLVGGAT
                     TSKQHTAVKIAPAYSQPTLHVLDASRVVGVVSGLLDPGRRGALVADARADQERLRGLH
                     EERLRKPLLPLAHARARRTPIEWRAEDLPEPPFTGTRIVEPDLAVLRRYIDWQFFFHA
                     WELKGRFPALLDDPEKGPAARELFDAANELLDEIVAGGLLTARGVYGFWPAQADGDDV
                     VLGNGVRFRLLRQQAAHEDGRPSRSLADFVAPAETGLADHVGAFAVTAGIGADELAAR
                     YEEDGDDYSAIIVKALADRLAEAFAERLHEEARREWYEAGPELSNEDLIAERFRGIRP
                     AFGYPACPDHSEKRTLFDLLEAEEAGITLTETFAMIPGASVSGLYLAHPEARYFSLGR
                     IGRDQVEDYARRKGVGVEQVERWLSAHLAY"
BASE COUNT          434 a         1196 c         1251 g          506 t
ORIGIN      
        1 cgcacggtca cgcggtgccc gagctcggcg aagcaggcgg ccgtgacgag gccgacccag
       61 ccggcgccga tcaccccgag cggttcccga tccatcgtcg aagcttagtt cgcgttcagg
      121 ggtttcaacc ccttggcgat ctcgagcatc gtctcgttcg agagctggtc gagaagcgtg
      181 ttcacgaccc agtaggcggc gccgttctcc tcgaacgcga ccatgtggag cttcggcccg
      241 ttgaagtaga gccggtagtc gcggccggag atcgtccgcg tgagcgtggc gccctcgagc
      301 gccggcgcct cggtgaagct cgtctgctgg atgccccagt agtcgagctc ggtcggcccg
      361 ctgtagacga accggaccgc gccctcgccc cggatcctgt acgtgcgcat cggctgcagg
      421 gtcgagaggt acgacgcgct ctcccggatc gtcgggacga gcaccgggaa gtcgaccttg
      481 cgctgcgcct cccgcgcctg ggcgaccgcg tgctcggggt cggtgacgac gacgggcggg
      541 gcggcctcgg gggtcgtgtc gcgcggcgcc ggggcgaggt tcccgtggaa cgtgcgcccg
      601 acgatcacgc ggagggtcgt gtcgagcggc gcgtccggcg tcgccggcac gagctcggcg
      661 tcgccgaaga gcctcgccat ctggttcgcc gcgccctcgg agccgcggac ggccgggtcg
      721 taggtcacct gcgtctcgaa gtagtcgagc cggtcggcgt tcccgccgtt caccgcctgg
      781 tagccgcggc ccgcgagcgc gacggcggcg tcgtccgcgg cgcccgcgac gccgttcccg
      841 ttcaggacgg tgaccgtgac ctgcgacggg ggcgggccgg tgcgccggtt cgcgggcgtc
      901 ttcccgccga ccgcgacgct cgtggccttc gccgccgcgt ccggatcggg gttcatgaac
      961 tcgtcgaccg cgcgctcgat ctcgccctcg ggcgcggaca gctggaagaa ctcgttgtcc
     1021 acgaggttct cgagccgcgg ctggaagagg ttcccgctcg gcagctcgta cgcgagccgg
     1081 gcgtagctga tgatcgtgtc gaggctcagc tccttcccgc ccccggagcc gatcgtgacg
     1141 tgcttcgcaa ccgtgttcac gatcccgggc agccgccagg cggcggagag cccgctcacc
     1201 tggtgcttga tcgacttcac gaactcctgc tggcgggcgt tgcggtagaa gtcgttgtcc
     1261 gtgtggcggt agcggacgaa cgcgagggcg tcgcggccgt cgaggagctg gtagcccggc
     1321 cggatgtcga tcgccgagta cgaggcgccg ccctgggagt tgtcgttgaa gtatcggcgg
     1381 tcgatgtcca tgtacacgcc gccgagctgg ttcaccgtgt cgatgaaccc gttgaagtcg
     1441 atcgtgacgt agtagttgat cgggacgccg gtcagcttgc ggacggtctc gatcgtcccc
     1501 ttgatcccgc agtccgtgaa cgcctcgttg atcttcgcca tccgcggggg cgccccctcg
     1561 cacccgggca gctcgacgag gaggtcgcgc gggaacgaga gcatcgagat cgtcttctgg
     1621 cgcgggtcgg cgcgcaggag catgagcgtg tcggagcggg actcctcccg ctcgaactcg
     1681 ccgtggcgga agtcgtagcc gaccacgagc gcgacggccg gctgatccgc gggcggcacc
     1741 tggtcgagga cctcctgcgc ctcccgctcc tccggcgtct gcggccgcgt cgaggcgctc
     1801 agctcctcgc cccagagcca gaacccgccg gcgacgcccg cgacggtgac gaggagcgtg
     1861 acgaggagcc acgcgaacac gcggccgacc acgcgcagcg gcgagcggcg ccgcggcgcg
     1921 tagcgggtca tcggcgtgag cggcggctgc ggcgggcgcg ggggctccgc gaccgccggt
     1981 gcggccccgt tcccgttcgg gattccgttc ccggaggccg cgttcgcgcg gctcacgcct
     2041 ctcttgagcg tcgtcctcat aggagaagcc gccccggggc gaccggtcga ggataccgga
     2101 gggatcggag aggatcggtc agtacgcgag gtgggccgag agccatcgct cgacctgttc
     2161 gacgccgacg cccttgcgcc tcgcgtagtc ctcgacctgg tcgcggccga tgcggccgag
     2221 cgagaagtag cgcgcctccg gatgcgcgag gtagagcccg ctgacgctcg cgccggggat
     2281 catggcgaag gtctccgtga gcgtgatccc ggcctcctcg gcctcgagca ggtcgaagag
     2341 cgtgcgcttc tccgagtggt cggggcaggc cgggtagccg aacgccggcc ggatcccgcg
     2401 gaagcgctcc gcgatcaggt cctcgttcga gagctcgggg ccggcctcgt accactccct
     2461 ccgtgcctcc tcgtggagcc tctcggcaaa cgcctcggcg agccggtcgg cgagcgcctt
     2521 cacgatgatc gcgctgtagt cgtcgccgtc ctcctcgtag cgggcggcga gctcgtccgc
     2581 gccgatcccc gccgtgaccg cgaacgcgcc cacgtggtcg gcgaggcccg tctcggccgg
     2641 cgcgacgaag tcggctagcg agcggctcgg gcgcccgtcc tcgtgcgcgg cctgctgccg
     2701 gagcagccgg aagcgcaccc cgtttccgag cacgacgtcg tcgccatccg cctgcgcggg
     2761 ccagaagccg tagacgcccc gcgcggtcag gaggccgccg gcgacgatct cgtccaggag
     2821 ctcgttcgcc gcgtcgaaga gctcgcgcgc ggccggtccc ttctccgggt cgtcgaggag
     2881 cgccgggaac ctccccttga gctcccaggc gtggaagaag aactgccagt cgatgtagcg
     2941 gcggagcacg gcgaggtccg gctcgacgat gcgggtgccc gtgaacggcg gctccggaag
     3001 gtcctcggcc cgccactcga tcggcgtccg ccgcgcccgt gcgtgggcga gcgggaggag
     3061 cggcttgcgg agccgctcct cgtgcaggcc gcgcagccgc tcctggtcgg cgcgcgcgtc
     3121 cgcgacgagc gccccgcgcc gcccggggtc gagcagcccg ctcacgacgc cgacgacgcg
     3181 cgacgcgtcg aggacgtgga gcgtcggctg cgagtacgcg ggcgcgatct tcaccgcggt
     3241 gtgctgcttc gaggtcgtgg cgccgccgac gaggagcggc aggtcgagcc cgcgccgctc
     3301 catctcctcg gccacgtgca ccatctcgtc gagcgacggg gtgatgaggc cggagagccc
     3361 gaccgcgtcc gcgccctccg cctcggc
//
LOCUS       DASTFC010000361         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1132685,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000361 DASTFC010000000
VERSION     DASTFC010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132685"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VFO88_10630"
     CDS             complement(<1..1009)
                     /locus_tag="VFO88_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546018.1"
                     /translation="MLDLPALDVAVGLVFLYVVLSLVCSTVNEIVATSVGLRARFLQM
                     GILNLLSAAPRPTRAGVETAKAFYAHPLVQGLIRPAHGPDPSHDPTSTARWWRKPPYP
                     SYIPSRTFVAALSDLLEDAEGRIAAADEEEAKAVEARLKGAAAELEQKLAAVPNARLS
                     EALLSLYRAAGADAAAFKHATEEWFDDAMERVSGWYRRRIQVILAVIATLVVLLLNAD
                     TLASGRVLWRDDAVRASVVRQAEASAEAGRDAAEVDDAVQRLDLPLGWDLSFGDRPTQ
                     VPDDPLGWIAKLVGLALTVGAVLLGAPFWFDLLSKIVRVRATGAPPPATDAVRAGEGD
                     QK"
     gene            1124..1876
                     /locus_tag="VFO88_10635"
     CDS             1124..1876
                     /locus_tag="VFO88_10635"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9546019.1"
                     /translation="MTRLDGKRGLVTGAASGIGKATAERLAEEGAAVLATDIQDDAGE
                     AVAAGIRERGGHAVFLHHDVSSEDEWIAAVERATGELGGLDILVNNAGIGDLHGIENT
                     SKEDYDRTIAITQTSVFLGMKHAGDALKASGNASVVNVSSIFGASGGFGGSPAYHAAK
                     GAVRILTKNVALRWATEGVRVNSVHPGFIDTPIIEQAMGTPIEEAMLEATPMGRLGRP
                     EEIAAVIAFLASDDASFMTGSEVYVDGGYLAR"
     gene            complement(1873..>2070)
                     /locus_tag="VFO88_10640"
     CDS             complement(1873..>2070)
                     /locus_tag="VFO88_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET9546020.1"
                     /translation="AIVRLVVLALLVLYALVFVLMNRDDVEVSFVFFTATTPLIVALV
                     LVGLIGGVAGWLARGQARRRV"
BASE COUNT          333 a          730 c          771 g          236 t
ORIGIN      
        1 gcttctggtc gccctcaccg gcgcggaccg cgtccgtcgc gggaggcggc gccccggtcg
       61 cgcggacccg gacgatcttg ctcaggaggt cgaaccagaa cggcgcgccg agcagcaccg
      121 cgccgaccgt gagcgcgaga ccgacgagct tcgcgatcca gccgagcggg tcgtcgggca
      181 cctgcgtcgg ccggtcgccg aaggagagat cccagcccag cgggaggtcg aggcgctgca
      241 ccgcgtcgtc gacttccgcc gcgtcgcggc ccgcctcggc ggatgcctcg gcctgccgca
      301 cgacggacgc ccggaccgcg tcgtcgcgcc agagcacgcg cccggaggcg agcgtgtccg
      361 cgttcaggag gaggacgacg agcgtcgcga tcacggccag gatcacctgg atccgccgcc
      421 ggtaccagcc ggacacgcgc tccatcgcgt cgtcgaacca ctcctccgtc gcgtgcttga
      481 acgccgcggc gtccgccccc gccgctcggt agagcgagag cagcgcctcc gagagccgcg
      541 cgttcggaac cgccgcgagc ttctgctcga gctccgccgc cgcccccttc aggcgcgcct
      601 cgaccgcctt cgcctcctcc tcgtcggccg cggcgatccg cccctcggcg tcctcgagca
      661 gatcggagag cgccgcgacg aaggtccgcg acgggatgta cgacgggtac ggcggcttgc
      721 gccaccagcg cgccgtgctc gtggggtcgt gcgaggggtc ggggccgtgc gccgggcgga
      781 tcagcccctg cacgagcgga tgggcgtaga acgccttcgc cgtctcgacc ccggcgcgcg
      841 tcggcctcgg cgccgcgctc agcaggttga ggatccccat ctggaggaac cgcgcgcgca
      901 ggcccaccga ggtcgcgacg atctcgttca cggtcgagca gacgagcgag aggacgacgt
      961 agaggaagac gaggccgacg gcgacgtcga gcgcggggag gtccagcacg aaaccgacgg
     1021 tacgccgcgg accggacgaa tcgtccgtcc cgtcgacggt tgcgccccgg cgcggccgga
     1081 aagcggagac tcgaccccgg aaacccggac aagaaggagc cgcatgacga ggttggacgg
     1141 aaagagaggg ctcgtcacgg gcgcggcgag cgggatcggc aaggcgaccg ccgaacggct
     1201 cgcggaggag ggcgcggcgg tgctcgcgac ggacatccag gacgacgccg gcgaggcggt
     1261 cgcggccggc atccgcgagc gcggcgggca cgcggtcttc ctccaccacg acgtgtcctc
     1321 ggaggacgag tggatcgccg ccgtcgagcg cgcgacgggc gagctcggcg ggctggacat
     1381 cctcgtgaac aacgccggga tcggcgacct gcacgggatc gagaacacct cgaaggagga
     1441 ctacgaccgg acgatcgcga tcacgcagac gagcgttttc ctcggcatga agcacgccgg
     1501 cgacgcgctc aaggcgtccg gtaacgcgtc cgtcgtgaac gtcagctcga tcttcggcgc
     1561 gagcggcggc ttcggcggct caccggccta ccacgccgcg aagggtgccg tccggatcct
     1621 cacgaagaac gtcgcgctcc gctgggcgac ggagggcgtg cgcgtcaact ccgtgcaccc
     1681 ggggttcatc gacacgccga tcatcgagca ggcgatgggc accccgatcg aggaggcgat
     1741 gctcgaggcg acgccgatgg gccgcctcgg ccggccggag gagatcgccg ccgtgatcgc
     1801 gttcctcgcg agcgacgacg cgtccttcat gaccggctcg gaggtctacg tggacggcgg
     1861 ctacctggcg cgctagaccc ggcggcgggc ctgcccgcgc gcgagccagc cggcgacccc
     1921 gccgatcagg ccgacgagga cgagcgcgac gatgagcggc gtcgtcgcgg tgaagaagac
     1981 gaagctcacc tccacgtcgt cgcggttcat gaggacgaag acgagggcgt agaggacgag
     2041 cagcgcgagg acgacgaggc ggacgatcgc
//
LOCUS       DASTFC010000362         7136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_572627,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000362 DASTFC010000000
VERSION     DASTFC010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572627"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFO88_10645"
     CDS             <1..603
                     /locus_tag="VFO88_10645"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HET9546021.1"
                     /translation="MTMRRDAIYPSIVVGIPPQEDAWLGKATERIFLPAVRMTVPEIV
                     DYDLPVAGAFHNCVIVSIRKAYPGHARKVMHAIWGLGMLSLSKAVVVVDDFVDVHDYE
                     QVFFHVCANVDPKRDVLLSEGPLDQLDHAAAGYCIGGKIGIDATHKTEAEGGRPWPER
                     IEMSTEIRDLVDRRWSEYGIDREAAGNGRISKDLRRKLRL"
     gene            673..1602
                     /locus_tag="VFO88_10650"
     CDS             673..1602
                     /locus_tag="VFO88_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9546022.1"
                     /translation="MADDLERPGGDAEPEAPPHAAPPSIWPFAFALGIAVALIGLVIS
                     WPIAAIGGAIALVFGFLWIRDATREVRGTPPPAPPARDDEAVELEDEAAARYPRSVFL
                     EGATLGIGALIGGIVTVPALGFAVLPAFVDQAYDAVDLGPLDNFPEGEWRIAKFKSVA
                     DEPEDVSRRTAYVRNNGTLDGQASFTIISNRCVHLGCPVQPQGLTADEVTEIDAESGP
                     VEITATEPSGFVCPCHGGAYDIEGNRTAGPPVRALDRYAYSIMDGRLVLGEPYSVGSV
                     EGEGAEAQIKAYKIADPGVHVDGLEQYFYPYVP"
     gene            1613..2437
                     /locus_tag="VFO88_10655"
     CDS             1613..2437
                     /locus_tag="VFO88_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET9546023.1"
                     /translation="MARTRSKRREQMEALVLAPVNWLEERSGIVGAISYFLFRKVPRG
                     TNWFHTLGSATLTAFIVQLVTGVVLAMYYVPDPDKAWDSINWITNELTLGWLVRGTHR
                     WGASVFIILLFLHMGRVFLFGAYKYPRELNWLIGVAILLLAMLEGFTGYLLPWDQTAY
                     WATVVGININATAPIAGPFIADFLRGGTEIGADTLSRFYAMHMLLVPGALIGLITLHL
                     YLVIRLGVTPPPWGEDAEPDEPSPNGRPRGAREGLVDPKPRGNGGPATTTRSGVEE"
     gene            2438..3247
                     /locus_tag="VFO88_10660"
     CDS             2438..3247
                     /locus_tag="VFO88_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9546024.1"
                     /translation="MAKLDNRERHARYKEDVEKRGKPFYPWAMFHDTVMSLVVVSVIT
                     ALAAIWYFTSGEEPGDAGLLGPRYTAEADPGTTSFVPRPDWYFFFLFYLLRIFKWPES
                     VILGTVGIPTILLILLFALPFIDRRRERRLVRRPVAVVAALLVIASMGILTWEGATAE
                     EPSATAGSVLCEANDLDCEGDEELALGADVFAEAGCLSCHVYAGEGAQNIGAPELTTV
                     GDLGRGEEYWVDWVRDPQAIRPGSPMPGFPNLTEEQLQAVAVFLEASTGGG"
     gene            3258..3842
                     /locus_tag="VFO88_10665"
     CDS             3258..3842
                     /locus_tag="VFO88_10665"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HET9546025.1"
                     /translation="MRVFLGITGASGAPYAARLLSALVDAGCEVGVSASAAGYEVLAT
                     ELYGDASLPHDEVRDRLVGGLDGVTVYAGNDYSAPYASGSAKVDGYVVCPCSMATLGT
                     LASGAMDNLVHRAASVALKEGRRLVLMPRETPLSAIHLEGMLKLRQAGAVILFLAPGF
                     YHEPKTIDDLVDFVVARALDQLGLDQTLVRRWGQ"
     gene            3839..4531
                     /locus_tag="VFO88_10670"
     CDS             3839..4531
                     /locus_tag="VFO88_10670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9546026.1"
                     /translation="MSVETGRLPSEDVRRMFDRISPVYDLMNRVMTAGLDRRWRRATV
                     AAVVRPGDRVLDACCGTGDLAVEASRAGARVVGLDFSGEMLARARRKSSAVEWVEGDV
                     LALPFEDGAFDAVTVGFGIRNVADLDAGLAELARVVRPGGRLGCLEITRPRGLLRPFF
                     RLWFDRIVPLLGKVLPGGAAYTYLPASVRRFPGPEDLAAALERAGFGAVSWRLFGGGI
                     VALHVGTREAGE"
     gene            4528..5448
                     /locus_tag="VFO88_10675"
     CDS             4528..5448
                     /locus_tag="VFO88_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9546027.1"
                     /translation="MSALAAVRAAAGLDAYLELLELRLERAVGSHPGVVGRIGTETLA
                     AGGKRLRPVLVFLATPPELREDERPLAAGTAVELVHMATLVHDDLLDAAALRRGRPTV
                     WAAHGADAARAAGDYLFARAFAELAATGDARAVSLLAGAALSLARGEALQRRQARRPD
                     TTVEEYLERCSLKTGKLFEAACLLGGGRDGLGAFGLALGIAFQIADDILDCAGTPGAT
                     GKPRGLDLRDGTPTLPLILAAREDRKIARALAGEDVPDALARVAATGALVEAREVALG
                     YARAARAALDGEYERDTLEPLTRVVVDREA"
     gene            5448..6557
                     /gene="mqnE"
                     /locus_tag="VFO88_10680"
     CDS             5448..6557
                     /gene="mqnE"
                     /locus_tag="VFO88_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HET9546028.1"
                     /translation="MATLAHGTALDEVRAKLEAGERLDFDDGLSLMESDDVLALGELA
                     DLARRLRGGTDDVYFVQNLYLNQTNVCRVKCKFCAFAVTRKQADAYTHTPDELVEDAL
                     RQRELTAFTEIHMVGGESPHVDFGYYVDIVAKLHEAMPDVHLKCFTASEIHHMTKLSG
                     LTHEQVLAELKAAGLGSLPGGGAEVFADRVRRLIAPGKEHPDFWFHTHRSAHGLGIPT
                     HCTMLYGHVETYEERVDHLLRLRDQQDETGGFLAFIPLAFHPENTVFERRGWTHTAGS
                     DDLKMMAVSRLLLDNVPNVKAYWIMMGMPLAAVALHFGANDVQGTVGREEIFHAAGAT
                     TETEQKIEELVRFVREAGRVPVQRDTLYGELRRWD"
     gene            6557..>7136
                     /locus_tag="VFO88_10685"
     CDS             6557..>7136
                     /locus_tag="VFO88_10685"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9546029.1"
                     /translation="MAETETQARTPTHEAVDARDWSFGGTWPYAPRWLFTDGIRIHYV
                     DEGPREGEPVLLLHGSPTWSYLYRRYVRGLAEAGFRAVAYDQLGFGRSDKAQRRREYS
                     LERHGRHLAALADELQLDGVTLMVHDWGGPIGLTWAAAHPERVRRLVVLNTFSQEAPG
                     ARGPFRWPLTRELLVKGAHFYVRGFLFRGGLRR"
BASE COUNT          962 a         2573 c         2592 g         1009 t
ORIGIN      
        1 atgacgatgc gccgcgacgc gatctacccc tcgatcgtcg tcgggatccc gccgcaggag
       61 gacgcctggc tcggcaaggc gaccgagcgg atcttcctcc cggcggtgcg gatgaccgtg
      121 ccggagatcg tcgactacga cctcccggtc gcgggcgcgt tccacaactg cgtgatcgtc
      181 tcgatccgca aggcctaccc cggccacgcg cgcaaggtca tgcacgcgat ctgggggctc
      241 gggatgctct cgctctcgaa ggcggtcgtc gtcgtggacg acttcgtcga cgtgcacgac
      301 tacgagcagg tcttcttcca cgtctgcgcg aacgtcgacc cgaagcgcga cgtccttctc
      361 tccgagggcc cgctcgacca gctcgaccac gccgcggcgg gctactgcat cggcgggaag
      421 atcggcatcg acgcgacgca caagaccgag gcggagggcg gccggccgtg gccggagcgg
      481 atcgagatga gcaccgagat ccgcgacctc gtcgaccgcc gctggagcga gtacggtatc
      541 gaccgggaag cggccgggaa cggccggatt tccaaggact tgcgccgaaa gctacgtctt
      601 tgacagaccg ccggaaagca ggagagaatc ccgcgtgccc gtaagcggat cggcaagcct
      661 ggagggggct gagtggcgga cgacctagag cggcccggcg gcgacgccga gcctgaagcg
      721 ccgccccacg ctgcgccgcc gtccatctgg ccgttcgcgt tcgcgctcgg catcgcggtc
      781 gccctcatcg ggctcgtgat ctcgtggccg atcgcggcga tcgggggcgc gatcgccctc
      841 gtcttcggct tcctctggat ccgcgacgcg acgcgcgagg tccgcgggac gccgccccca
      901 gccccgccgg cgcgtgacga cgaggcagtc gagctcgagg acgaggccgc ggcgcgttac
      961 ccgcgctccg tcttcctcga gggcgcgacg ctcgggatcg gcgcgctgat cgggggcatc
     1021 gtcaccgtcc cggcgctcgg gttcgccgtg ctcccggcct tcgtcgacca ggcgtacgac
     1081 gctgtcgacc tcggcccgct cgacaacttc ccggagggcg agtggcggat cgcgaagttc
     1141 aagtccgtcg cggacgagcc ggaggacgtg tcgcgccgca ccgcgtacgt ccgcaacaac
     1201 ggcacgctcg acggccaggc gagcttcacg atcatctcga accggtgcgt ccacctcggg
     1261 tgccccgtgc agccgcaggg gctgacggcg gacgaggtga cggagatcga cgccgagtcc
     1321 ggcccggtcg agatcaccgc cacggagccg tccgggttcg tctgcccgtg ccacggcggc
     1381 gcgtacgaca tcgaagggaa ccggacggcc ggcccgcccg tccgcgcgct cgaccgctac
     1441 gcgtactcga tcatggacgg ccggctcgtg ctgggcgagc cgtacagcgt cgggagcgtc
     1501 gagggcgagg gcgcggaggc gcagatcaag gcgtacaaga tcgccgaccc gggcgtccac
     1561 gtcgacgggc tcgagcagta cttctatccc tacgtcccgt gagcgagggc tgatggcgcg
     1621 cacacgatcg aagcgacgcg agcagatgga ggcgctcgtt ctcgcgcccg tgaactggct
     1681 cgaggagcgc tccgggatcg tcggcgcgat cagctacttc ctcttccgga aggtcccccg
     1741 cgggacgaac tggttccaca cgctcggctc ggcgacgctc accgcgttca tcgtccagct
     1801 cgtcaccggg gtcgtgctcg cgatgtacta cgtgccggac cccgacaagg cctgggactc
     1861 gatcaactgg atcacgaacg agctgacgct cgggtggctc gtccgcggca cgcaccggtg
     1921 gggggcgagc gtcttcatca tcctgctctt cctccacatg ggccgcgtgt tcctgttcgg
     1981 cgcgtacaag tacccccgcg agctgaactg gctgatcggg gtcgccatcc tgctcctcgc
     2041 catgctcgag ggcttcacgg gctacctgct cccctgggat cagaccgcgt actgggcgac
     2101 cgtcgtcggg atcaacatca acgcgaccgc gccgatcgcc gggccgttca tcgcggactt
     2161 cctccgagga ggaacggaga tcggagcgga cacgctgtca cggttctatg ccatgcacat
     2221 gctcctggtc ccgggcgccc tcatcgggct gatcacgctg cacctgtacc tcgtgatccg
     2281 gctcggcgtg acgccgccgc cgtggggcga ggacgccgag ccggacgagc cctcgccgaa
     2341 cggccggccg cggggcgcgc gcgaagggct cgtcgacccg aagccgcgcg ggaacggcgg
     2401 cccggcgacc acgacgcgca gcggagtcga ggagtagatg gccaagctcg acaaccgcga
     2461 gcgccacgcc cgctacaagg aggacgtcga gaagcgcggg aagccgttct acccgtgggc
     2521 gatgttccac gacacggtga tgagcctcgt cgtcgtctcc gtgatcacgg cgctcgcggc
     2581 gatctggtac ttcacgtcgg gcgaggagcc gggcgacgcg ggcctgctcg ggccgcgcta
     2641 cacggccgag gccgacccgg gcacgacgtc gttcgtgccg cggccggact ggtacttctt
     2701 cttcctcttc tacctcctcc ggatcttcaa gtggccggag tcggtgatcc tcggcacggt
     2761 cgggatcccg acgatcctcc tcatcctcct cttcgcgctc ccgttcatcg accggcggcg
     2821 cgagcggcgg ctcgtccggc ggcccgtcgc ggtcgtcgcg gcgctcctcg tgatcgcgtc
     2881 gatggggatc ctcacgtggg agggcgcgac ggcggaggag ccgagcgcga ccgccggctc
     2941 ggtgctctgt gaggcgaacg acctcgactg cgagggggac gaggagctcg cgctcggggc
     3001 cgacgtgttc gccgaggccg ggtgcctgag ctgccacgtc tacgcggggg agggcgcgca
     3061 gaacatcggc gcgccggagc tcacgaccgt gggcgacctc ggccgcggcg aggagtactg
     3121 ggtcgactgg gtgcgcgacc cgcaggcgat ccggccgggc tcgccgatgc cgggcttccc
     3181 gaacctgacc gaagagcagc tccaggcggt cgccgtcttc ctcgaggcct cgacgggcgg
     3241 cgggtagcgc ccgcgccgtg cgggtgttcc tcggcatcac gggcgcctcg ggcgcgccgt
     3301 atgccgcccg gctcctctcg gcgctcgtcg atgcgggctg cgaggtcggc gtgtcggcct
     3361 cggcggcggg ctacgaggtg ctcgccacgg agctctacgg agacgcgtcg ctcccgcacg
     3421 acgaggtgcg cgaccggctc gtgggcggcc tcgacggcgt gaccgtctac gcggggaacg
     3481 actactccgc gccgtacgcg agcggctcgg ccaaggtgga cggctacgtg gtctgcccgt
     3541 gctcgatggc gacgctcggg accctcgcgt ccggcgcgat ggacaacctc gtccaccggg
     3601 ccgcgtcggt cgcgctcaag gaggggcgga ggctcgtgct catgccgcgc gagacgccgc
     3661 tctcggcgat ccatctggag gggatgctga agctccggca ggccggggcc gtgatcctct
     3721 tcctcgcgcc cggcttctac cacgagccga agacgatcga cgacctcgtc gacttcgtcg
     3781 tcgcccgggc gctcgaccag ctcgggctcg accagacgct cgtgaggcgt tgggggcagt
     3841 gagcgtggag acgggccggc tcccctcgga ggacgtccgc cggatgttcg accggatctc
     3901 gccggtctac gacctcatga accgggtcat gacggccggc ctcgaccggc gctggcggcg
     3961 ggcgaccgtg gcggcggtcg tccggcccgg cgaccgggtg ctggacgcgt gctgcgggac
     4021 gggcgacctc gccgtcgagg cgtcgcgggc gggcgcgcgc gtggtcgggc tcgacttctc
     4081 cggggagatg ctcgcgcggg cgcggcggaa gtcctcggcg gtcgagtggg tcgagggcga
     4141 cgtgctcgcg cttccgttcg aggacggggc gttcgacgcc gtgaccgtcg gcttcgggat
     4201 ccggaacgtc gccgatctcg acgccgggct cgccgagctc gcgcgcgtcg tccggccggg
     4261 cgggcggctc gggtgtctcg agatcacgcg gccgcgcggg ctcttgcggc cgttcttccg
     4321 tctctggttc gaccggatcg tgccgctcct cgggaaggtg ctgcccggcg gggccgcgta
     4381 cacgtacctt ccagctagcg tgcgacgctt tccggggccc gaggacctcg ccgccgcgct
     4441 cgaacgggcg ggcttcggcg ccgtttcgtg gcgcctcttc ggcggcggga tcgtcgcgct
     4501 gcatgtcggg acgcgggagg ccggggagtg agcgcgctcg ccgccgtccg cgccgcggcg
     4561 gggctcgacg cgtacctcga gctgctcgag ctgcggctcg agcgcgcggt cggctcgcac
     4621 ccgggcgtcg tcgggcggat cgggacggag acgctcgcgg ccggcggcaa gcgcctccgg
     4681 ccggtgctcg tcttcctcgc cacgccgccc gagctgcgcg aggacgagcg accgctcgcg
     4741 gcgggcacgg cggtcgagct cgtgcacatg gcgacgctcg tccacgacga cctgctcgac
     4801 gcggcggcgc tccggcgcgg gcgcccgacc gtgtgggccg cgcacggggc cgacgccgcg
     4861 cgggcggccg gcgactacct cttcgcgcgc gccttcgccg agctcgccgc cacgggcgac
     4921 gcgcgggcgg tctcgctcct cgcgggcgcg gcgctctcgc tcgcgcgcgg cgaggcgctc
     4981 cagcggcgcc aggcgcgccg gccggacacg accgtcgagg agtacctcga gcgctgctcg
     5041 ctgaagacgg ggaagctctt cgaggccgca tgcctcctcg gcggcggccg cgacgggctc
     5101 ggcgcgttcg ggctcgcgct ggggatcgcg ttccagatcg cggacgacat cctcgactgc
     5161 gccggcacgc ccggggcgac gggcaagccg cgcggcctcg acctgcgcga cgggacgccg
     5221 acgctgccgc tcatcctcgc ggcgcgcgag gaccggaaga tcgcgcgcgc gctcgccggc
     5281 gaggacgtgc cggacgcgct cgcccgcgtc gccgcgaccg gcgccctcgt cgaggcgcgt
     5341 gaggtcgccc tcggctacgc gcgcgccgcc cgtgcggcgc tcgacggcga gtacgagcgg
     5401 gacaccctcg agccgctcac ccgcgtcgtg gtagacaggg aggcgtgatg gcgacgctcg
     5461 cgcacggcac cgcgctcgac gaggtccgcg cgaagctcga ggcgggggag cggctcgact
     5521 tcgacgacgg gctcagcctc atggagtcgg acgacgtcct ggccctcggc gagctcgccg
     5581 acctcgcgcg gcggctgcgc ggcggcacgg acgacgtcta cttcgtccag aacctctacc
     5641 tgaaccagac gaacgtctgc cgcgtgaagt gcaagttctg cgcgttcgcc gtcacccgga
     5701 agcaggcgga cgcctacacg cacacccccg acgagctggt cgaggacgcg ctccggcagc
     5761 gcgagctcac cgccttcacc gagatccaca tggtcggcgg cgagagcccg cacgtcgact
     5821 tcggctacta cgtggacatc gtcgcgaagc tccacgaggc catgcccgac gtccacctca
     5881 agtgcttcac ggcgagcgag atccaccaca tgacgaagct ctccgggctc acgcacgagc
     5941 aggtgctcgc ggagctcaag gcggcggggc tcggctccct ccccggcggc ggcgccgagg
     6001 tgttcgccga ccgggtgcgc cgcctgatcg cgcccggcaa ggagcacccg gacttctggt
     6061 tccacacgca caggagcgcg cacgggctcg gcatccccac ccactgcacg atgctctacg
     6121 gccacgtgga gacctacgag gagcgcgtcg accacctcct gcgcctgcgc gaccagcagg
     6181 acgagacggg cggctttctc gccttcatcc cgctcgcctt ccacccggag aacaccgtct
     6241 tcgagcgccg cggatggacg cacacggccg gctccgacga cctcaagatg atggccgtct
     6301 cgcggctcct cctcgacaac gtcccgaacg tgaaggcgta ctggatcatg atgggcatgc
     6361 cgctcgcggc cgtcgcgctc cacttcggcg caaacgacgt ccagggcacg gtcgggcgcg
     6421 aggagatctt ccacgccgcg ggcgcgacga ccgagaccga gcagaagatc gaggagctcg
     6481 tgcgcttcgt gcgcgaggcg gggcgcgtgc cggtgcagcg ggacacgctc tacggcgagc
     6541 tgcggcggtg ggactagtgg cggagaccga gacgcaggct cgcaccccca cccacgaggc
     6601 cgtcgacgcc cgcgactgga gcttcggcgg aacgtggccg tacgcgcccc gctggctgtt
     6661 cacggacggc atccgaatcc actacgtgga cgaggggccc cgggagggcg agcccgtcct
     6721 gctgctccac gggagtccaa cgtggtccta cctctaccgc cgctacgtgc gtgggctcgc
     6781 cgaggcgggc ttccgcgccg tcgcctacga ccagctcggc ttcggccgct ccgacaaggc
     6841 gcagcgccgc agggagtact cgctcgagcg ccacggccgc cacctcgcgg ccctcgccga
     6901 cgagctccaa ctggacggcg tcacgctcat ggtccacgac tggggcgggc cgatcgggct
     6961 gacgtgggcc gccgcccatc cggagcgggt gcgccggctc gtcgtcctga acacgttctc
     7021 ccaggaggcg cccggcgccc ggggcccgtt ccgctggccg ctcacgcgcg agctcctcgt
     7081 caagggcgcg cacttctacg tacgcgggtt cctcttccgc ggcgggctgc gccggc
//
LOCUS       DASTFC010000363         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1413060,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000363 DASTFC010000000
VERSION     DASTFC010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413060"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFO88_10690"
     CDS             <1..328
                     /locus_tag="VFO88_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SSI family serine proteinase inhibitor"
                     /protein_id="HET9546030.1"
                     /translation="GPASALELTVWPEGQDAGRAESWTLECDPAGGTHPSPDAACSLL
                     ADNADLLEPLPDDVMCTQQFGGPEQAAIRGTFRGREVDLRHSRANGCEIARWERLAPV
                     LQADAS"
     gene            complement(325..1797)
                     /locus_tag="VFO88_10695"
     CDS             complement(325..1797)
                     /locus_tag="VFO88_10695"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET9546031.1"
                     /translation="MISYRRVDTGGDARSLAEALRRRFGNENVFLDIETIGPGEPFDQ
                     AIDRAVDSSDVLLALIGQHWLAVTDDEGRRRIDDPKDVVRVEILSALQRQLVVIPVLL
                     EDATMPSEEELPPDLAPFSKRNAVELRGTKWESDVESFVDDLAEERRKKFRRLGAGRG
                     WRRVVAPLGDAVWAVRRAAGKSPRVAAAVTLLLLLLLAALVAVLVLRNGDGSTGGSGG
                     GEALHPPLAEDETLAFVSGNRVVILDGDAKNPLPLTVADPSESVDWTADGRLVYSDGG
                     NLWIVEPDGKPQPLTTDRLGDGHPSVRKDDGLVAFNRPVGSDGKCPCGIWLVPADRSI
                     EPHPPGEGEPIPGVAPDWSSDGRRIAYQRRRDESGDQHIWFMDEDGQNQRELLPSSNE
                     PAMFPAWAPSGNRIAFWLPDESGCRIGIARPDQDVLEPELRFDTPATCSGPEWSPDGR
                     YLAVTAPDGIWAIRVDGTEDPQLLLDIESPKSLSWVRRRG"
     gene            complement(1860..2306)
                     /locus_tag="VFO88_10700"
     CDS             complement(1860..2306)
                     /locus_tag="VFO88_10700"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET9546032.1"
                     /translation="MAAESEDVTLQRLEDQIAWYDRKSGTAQRRFKLLKGSQLVFAAA
                     IPVVATLDFHSAVAGTLGGLIVVLEGMAQLNQYQQNWQAYRSTCEALKHEKYTFLALA
                     GPYASVQKPLPLLADRIEGLISQEHAKWVSAQEEAGRYGTSKEPDA"
     gene            complement(2490..3530)
                     /gene="proB"
                     /locus_tag="VFO88_10705"
     CDS             complement(2490..3530)
                     /gene="proB"
                     /locus_tag="VFO88_10705"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET9546033.1"
                     /translation="MRPVVVKLGTTLVAGPGGTVRRSLLRARAREIAELVRAGTPVCV
                     VSSGAIALGLPRLGLERRPRAVPRLQAASALGQSRLQKAWETALAPEKLAVAQVLLTA
                     ADLAERTAYVNVRNAIVELFRLGAVPIVNENDATATDEITFGDNDSLAAQVAVLARAR
                     LLVLLTEVEGVYSAAPGSPGAELVEEGGRAGGAAFGSGSALGRGGMESKVGAAQLAAG
                     AGIPTVIAGGRGESVLGPIIAGERRGTRFAPAESRESAFKLWLRYCKPVTGRLVVDDG
                     ARAAVARGGASLLAAGVVATEGEFGPGDGVVLVGANGEEFAKGIAEATPAELAGRPRG
                     VEAVHRDRLLLL"
     gene            complement(3530..>3867)
                     /locus_tag="VFO88_10710"
     CDS             complement(3530..>3867)
                     /locus_tag="VFO88_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546034.1"
                     /translation="WANDAERVSTATVELVDGLEHALAIANGETSGLAAGIVAEDEAA
                     AGQFLDGYRGSAAFWHAPTRFADGYELLGAPETGINVDSVPGPRGPVSYRDLWLRQYR
                     IVGDGTQRR"
BASE COUNT          520 a         1453 c         1371 g          523 t
ORIGIN      
        1 cgggcccgcg agcgccctcg agctgaccgt ctggccggag ggacaggacg cgggccgggc
       61 ggagtcgtgg acgctcgagt gcgacccggc cggcggtacg cacccctcgc ccgacgcggc
      121 ctgctcgctc ctcgcggaca acgccgacct cctggagccg ctcccggacg acgtcatgtg
      181 cacgcagcag ttcggcgggc cggagcaggc ggcgatccgc ggaacgttcc ggggccgcga
      241 ggtcgacctg cgccacagcc gcgcgaacgg gtgcgagatc gcgcgctggg agcgcctcgc
      301 gccggtccta caggccgacg cgagctagcc ccgacgccgg acccaggaca gcgatttggg
      361 gctctcgatg tccaggagca gctgcgggtc ctcggtgccg tcgacgcgga tagcccagat
      421 gccgtccggc gccgtgacag cgagataccg tccgtcggga gaccattcag gtccgctgca
      481 cgtcgccggc gtgtcgaacc tgagctcggg ttctagtacg tcctggtcgg ggcgggcgat
      541 gccgattcgg caacccgact cgtccgggag ccagaatgcg atccgattgc cgctcggcgc
      601 ccaggcaggg aacatcgctg gctcgttgga actcggaagg agctcgcgct ggttctggcc
      661 gtcctcgtcc atgaaccaga tgtgctggtc gcccgactcg tcgcgacgtc gctggtacgc
      721 gatcctgcgg ccgtccgacg accagtcggg cgccacgcca gggatcggct cgccctcgcc
      781 gggcgggtgc ggctcgatgg agcggtctgc aggaaccaac cagatcccgc atgggcattt
      841 cccgtccgac cccaccgggc ggttgaacgc gacgagccca tcgtcctttc ggacagaggg
      901 atggccgtcg cccaaccgat cggtcgtgag cggctgtggt ttcccgtccg gttcgacaat
      961 ccagaggttt ccgccgtcgg agtagacgag ccgtccgtcc gcagtccagt cgacgctctc
     1021 agagggatcg gcaacggtca gcggtagagg attcttcgcg tctccatcga ggatcacgac
     1081 gcggttgcct gagacgaacg cgagcgtctc gtcctcggcg agcggggggt ggagcgcctc
     1141 acccccgcca ctgccgcccg tgctgccgtc gccgttccgc aggacgagca ccgcgacgag
     1201 cgccgcgagg agcagcagga ggaggagcgt gaccgccgcc gcgacgcgcg ggctcttccc
     1261 cgccgcgcgg cggacggccc agaccgcgtc gccgagcggt gcgacgacgc gccgccaccc
     1321 gcggcccgcg ccgaggcggc ggaacttctt ccgccgctcc tcggcgaggt cgtcgacgaa
     1381 gctctcgacg tcggactccc acttcgtccc gcggagctcg accgcgtttc gcttcgagaa
     1441 cggcgcgagg tcgggcggga gctcctcctc ggagggcatc gtcgcgtcct cgagcaggac
     1501 ggggatgacg accagctgcc gctggagcgc ggagaggatc tcgacccgga cgacgtcctt
     1561 cggatcgtcg atgcgccggc ggccctcgtc gtccgtcacg gcgagccagt gctggccgat
     1621 cagcgcgagc aagacgtcgc tcgagtcgac cgcgcggtcg atcgcctggt cgaacggctc
     1681 gcccggaccg atcgtctcga tgtcgaggaa gacgttctcg ttcccgaacc gccgccggag
     1741 tgcctcggcg agggagcgcg cgtcgccgcc cgtgtcgacg cgacggtagc tgatcacgat
     1801 cctgaacgtc cgctgcgtag cagccgcggg cgccgcgggc gcaccggggc cgccggccgt
     1861 cacgcgtctg gctccttcga ggtcccgtag cggcccgcct cctcctgcgc ggagacccac
     1921 ttcgcgtgct cctgcgagat cagcccttcg atgcggtcgg cgagcagggg cagcggcttc
     1981 tgaacgctcg cgtacggccc ggccagcgcg aggaaggtgt acttctcgtg cttgagcgcc
     2041 tcgcacgtcg agcggtacgc ctgccagttc tgctggtact ggttgagctg ggccatcccc
     2101 tcgaggacga cgatgagccc gccgagcgtg ccggcgacgg cggagtggaa gtcgagcgtc
     2161 gcgacgaccg ggatcgccgc cgcgaagacg agctgcgagc ccttgaggag cttgaagcgc
     2221 cgctgcgcgg tcccgctctt gcggtcgtac caggcgatct ggtcttcgag acgctggagg
     2281 gtgacgtcct cggattcggc ggccacggcg gctcgattgt cccacaccgg gcagccggga
     2341 tcccgtacgc gcccgtggat tcacgcccgc gtgttctttc ccccggggga cccctccgag
     2401 tcacccctca ccgctcaggc tatcgatgag gggcgcacgc atggtgacgt gggcgtgccg
     2461 agttcgtgcc ggaccgtcca caggcgcgcc tagaggagga gcagccggtc gcggtgcacc
     2521 gcctcgacgc cgcgcgggcg gcccgcgagc tcggccgggg tcgcctcggc gatccctttc
     2581 gcgaactcct ccccgttcgc tccgacgagg acgacgccgt cgcccggccc gaactcgccc
     2641 tccgtcgcga cgacgcccgc ggcgagcagg ctcgccccgc cgcgcgcgac ggccgcgcgc
     2701 gccccgtcgt ccacgacgag ccggcccgtg accggcttgc agtagcgcag ccacagcttg
     2761 aaggccgact cgcgcgattc cgcgggcgcg aagcgcgtgc cgcgccgctc gccggcgatg
     2821 atcggcccga gcaccgactc gccccggccg ccggcgatca ccgtgggaat ccccgcgccg
     2881 gccgcgagct gggcggctcc gaccttcgac tccatcccgc cgcggccgag cgccgacccc
     2941 gacccgaacg ccgccccgcc cgcgcgaccg ccctcctcga cgagctcggc ccccggcgac
     3001 ccgggcgcag ccgagtagac gccctccacc tccgtgagca ggacgagcag cctggcccgc
     3061 gcgagcaccg ccacctgcgc ggcgagcgag tcgttgtcgc cgaacgtgat ctcgtccgtc
     3121 gcggtcgcgt cgttctcgtt cacgatcggg accgcgccga gccggaagag ctccacgatc
     3181 gcattgcgaa cgttcacgta cgccgtccgc tccgcgaggt cggccgcggt gaggagcacc
     3241 tgcgcgaccg cgagcttctc cggcgcgagc gccgtctccc aggccttctg gagccgcgac
     3301 tggccgagcg ccgacgccgc ctggagccgg ggcaccgccc gcgggcgccg ctcgagcccg
     3361 agccggggaa gcccaagcgc gatcgcgccg ctcgacacga cgcagacggg cgtcccggcg
     3421 cgcacgagct ctgcgatctc gcgggcccgc gcgcggagca gcgagcgccg caccgtcccg
     3481 cccggcccgg cgacgagcgt cgtcccgagc ttgacgacga ccggccgcat cagcgccgct
     3541 gcgttccgtc ccccacgatc cggtactgcc ggagccagag gtcgcggtag ctcaccgggc
     3601 cgcgcgggcc gggcacggag tcgacgttga tcccggtctc gggcgcgccg agcagctcgt
     3661 agccgtcggc gaagcgcgtg ggcgcgtgcc agaaggcggc cgagccgcgg tagccgtcga
     3721 gaaactgccc ggcggccgcc tcgtcctcgg ccacgatccc ggccgcgagc ccggacgtct
     3781 cgccgttcgc gatcgcgagc gcgtgctcga ggccgtccac gagctcgacc gtcgccgtcg
     3841 agacccgctc cgcgtcgttc gcccact
//
LOCUS       DASTFC010000364         7525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_572964,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000364 DASTFC010000000
VERSION     DASTFC010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572964"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..570)
                     /locus_tag="VFO88_10715"
     CDS             complement(46..570)
                     /locus_tag="VFO88_10715"
                     /inference="COORDINATES: protein motif:HMM:NF022798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3156 family protein"
                     /protein_id="HET9546035.1"
                     /translation="MRGRARAAAARSLELDLDTFEAVGYRRGEKAGPLAVRLEPPDGL
                     PPLVLRLAPEGRIFGGAFALEVSTAEPVLPPTQGLSARGRGVVRLARIAWRPRRGDAA
                     GAALARRLDEDAAIQEKLAAVHFERLRVEPDGRPVVRHMGGSVVWILFPPVVRPIPLT
                     PGQARATARALGAF"
     gene            complement(560..1819)
                     /locus_tag="VFO88_10720"
     CDS             complement(560..1819)
                     /locus_tag="VFO88_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9546036.1"
                     /translation="MAASPALDEIDLGNDAFVERIPHETFALLRREAPVWWYDWPGGR
                     GFWCVTKHADVVSVSRDTKTFSSAQGANLEDLDEEMMAARRSMLETDPPRHTRLRGLV
                     GPPFTPRAVKAYELALRELTREILDRALPLGEFDFVEEIAKELPIRVLARLLGTPESD
                     TDRLIDWGDRMIANTDPDYADVLHDSPESEAYRLVPFRSPAALELFEYGHALAAERRR
                     EPRDDLVSKLVHTEIDGERLTEREFDTMFLLLVVAGNETTRQAIAHGMLALVEHPDQW
                     ERLRGDPQLAWERGADEILRWSSPVLHFRRTATADTEIRGQPVAAGDKVVVWYVSANF
                     DEEVFEAPERFDVGRSPNRHLTFGGGGPHYCLGAHLAKLEVQVLFDELLPRLDALEVT
                     GPVERMRTNFTNAIKRMPVRVTARGAR"
     gene            1855..2211
                     /locus_tag="VFO88_10725"
     CDS             1855..2211
                     /locus_tag="VFO88_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9546037.1"
                     /translation="MESRRARGLARLAELGDEPGGEEFLARLGDLGDLTVDFVFGDVH
                     ARPGLGVRERELVIVAVLTALGGLDPQVRAHIRAAREAGVSEEELEETMIQVAPYAGF
                     PRAINGLKVLREEQGR"
     gene            2208..3038
                     /locus_tag="VFO88_10730"
     CDS             2208..3038
                     /locus_tag="VFO88_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9546038.1"
                     /translation="MRAARFVDPSGAERIGRYADEGVVDAGPAGVGGFVPSAEGWDEV
                     EAASGPEYALDEVRLLHPVVPAKLVGIGLNYREHAAESELDVPEVPVAFAIWPSALVG
                     PGAPIVVPRDETRPDYEGEVAIVLGRTVKNVSPEEGLAAIGGFAPFNDVSGRRAQLET
                     PLRQFTIGKSFDTFSPMGPCVASADGVDLDALDVRTTVSGEVLQEASTRDLIFDFGRI
                     VSYLSTGITLEAGTVVATGTPGGVGDTRDPRRYLEEGDVVEVFVSGAGTLTNPVERER
                     "
     gene            3061..3744
                     /locus_tag="VFO88_10735"
     CDS             3061..3744
                     /locus_tag="VFO88_10735"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HET9546039.1"
                     /translation="MASASRSRRRIAVLVPLALLSAFVVAGVVVRWRYTGSHEAANLP
                     WNLFLAWIPLGLALAVHDAHRGGARLAWLLAGGGLWLLFFPNAPYLVTDLKHLGAWSG
                     APEWFDVVLVSAAAWTGLLLGFASLYLVQAVVRELRGAAAAWAVVVGSIALGSFGIYL
                     GRFERWNSWDALTSPRPILADLWAGAMNPVAHPRVLAVTVLFTGFLTLAYLTFYGLVA
                     LGRNEREPL"
     gene            3744..4166
                     /locus_tag="VFO88_10740"
     CDS             3744..4166
                     /locus_tag="VFO88_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9546040.1"
                     /translation="MEQRVSLVTLGVHDLGRARRFYEALGWTTGAAPADDVAFFQAGG
                     MVVALWGREELAADSGVEDAGGWGGVTLAHNVRSPADVDAVVEEARAAGAAITREPAE
                     TFWGGYSGAFTDPDGHAWEIAHNPSWTLADDGSVRLPA"
     gene            complement(4341..4580)
                     /locus_tag="VFO88_10745"
     CDS             complement(4341..4580)
                     /locus_tag="VFO88_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546041.1"
                     /translation="MSAGNGSGPSFAADIRPLFREDDREAMEFAFDLFEYGDVRDNAA
                     AILERLEDGSMPCDGEWPEEQIARFRAWIDAGLAP"
     gene            complement(4577..5911)
                     /locus_tag="VFO88_10750"
     CDS             complement(4577..5911)
                     /locus_tag="VFO88_10750"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET9546042.1"
                     /translation="MGERLSAEERLAHVREAGCDRLRVHFTDLLGVSRNKVVPLTQLD
                     ECLEDGINFCISAFCVDHVGEVIEGTGLGAEVDFRDAQVIPDLDTLTVVPWERSTAIA
                     IGDVRFDGDWLPASPRQLLARASDDLGKRGFRAVAGHELEFFLLRPNGSGYEQYAKQP
                     GLVYTLSPSVDTEGVLRKMEDAVAAMGLPLIASNQEYFGSQWEINLRYDDALKAADDA
                     HLLKLAVKEIAAQHGLVATFMGKPIQELGTSGYHLHLSLWDDGTNAFDDPDGPDGLSE
                     VCRQFLAGQLEHARGMTAIMAPTINAYKRFLAMAFGPWNVTWGHDNRTVYARIPRERG
                     KATRIENRAGDGTANAYIASAAALFAGIDGLERELDPGEPVSGVAYELEDRTLMPFSL
                     GEALDALEEDTFFVEHLGPQFVQAFLAMKRSEVARFASAVTDWELNEYAVTL"
     gene            complement(5919..7388)
                     /locus_tag="VFO88_10755"
     CDS             complement(5919..7388)
                     /locus_tag="VFO88_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9546043.1"
                     /translation="MAARADQGGNGGLERRAVGLPTAIGTTFGLIVASTVLATVTAGF
                     FASYVFLAALAVGLLAMYLQSMSFAELATMIPKAGSMNEYVRAGLGPFFATLTVLVGY
                     IAVILFPTAAESYTPSAIIKGFLWTDGPSTTVWVIVFVVFVALLNIAGIRPYGAVEVG
                     LTAFVALSLLIFGLIGVLGLGTTDPIGGLFPDVPFTWSLFSTLLGIALFTFVGMEYTC
                     PLAEEMENPGRNIPLGIFLGLTLVSVPVVLFGLAAARYVPAEQLEAFSPVAHIETAIA
                     ILGDTGKWWMAVVSIAATLSTLNALIAGIPRILYGMGLTRQLPSVFAYLLPSTRAPVV
                     GILVIASLPILMNVVVDLGTSQNFIKLILAGVLGWATAYFLIHLSVVILRIVEPNAAR
                     PFRAPLVPIPQLVGSGLIALAAFKIFPVEEVRNDAYLYYGIFLAVAAAASFLMNAMGG
                     GLTSQFRRVPLAEVYRETELIEEDLPLPVEPGGPHIRHD"
BASE COUNT         1036 a         2672 c         2792 g         1025 t
ORIGIN      
        1 gaccgagcgc ggccgggcgc gcggcctcga ctggttgccg cgctactaga aggcgccgag
       61 cgcgcgggcg gtcgcgcgtg cctgcccggg cgtgagcggg atcgggcgca cgaccggggg
      121 gaagaggatc cagacgacgg agccgcccat gtggcggacg acggggcgtc cgtcgggctc
      181 gacgcgcagc cgctcgaagt gcacggcggc gagcttctcc tggatcgccg cgtcctcgtc
      241 gagccgccgg gcgagcgccg cgcccgccgc gtcgccgcgc cgtgggcgcc aggcgatccg
      301 ggcgagccgg acgacgccgc gcccgcgcgc cgagaggccc tgggtgggcg gcagcaccgg
      361 ctccgcggtc gagacctcga gggcgaacgc cccgccgaag atccgtccct cgggcgcgag
      421 ccggaggacg agcggcggca gcccgtccgg cggctcgagg cgcacggcga gcggccctgc
      481 cttctcgccg cgccggtagc cgaccgcctc gaaggtgtcg aggtcgagct cgagcgagcg
      541 ggcggcggcc gcgcgcgccc taccgcgcac cgcgcgccgt gacgcggacg ggcatccgct
      601 tgatcgcgtt cgtgaagttc gtccgcatgc gctcgaccgg gcccgtcacc tcgagcgcgt
      661 cgagccgcgg gagcagctcg tcgaagagca cctgcacctc gagcttcgcg aggtgcgccc
      721 cgaggcagta gtgcggcccg ccgcccccga acgtcagatg ccggttcggc gagcgcccga
      781 cgtcgaagcg ctcgggcgcc tcgaagacct cctcgtcgaa gttcgccgag acgtaccaga
      841 cgaccacctt gtcccccgcc gcgaccggct gcccgcggat ctccgtgtcc gcggtcgccg
      901 tccggcggaa gtggaggacg gggctcgacc agcgcaggat ctcgtccgcg ccgcgctccc
      961 aggcgagctg cgggtcgccg cggaggcgct cccactggtc ggggtgctcg acgagggcga
     1021 gcatcccgtg ggcgatcgcc tggcgcgtcg tctcgttccc ggcgacgacg aggaggagga
     1081 acatcgtgtc gaactcgcgc tcggtcagcc gctcgccgtc gatctccgtg tggacgagct
     1141 tcgagacgag gtcgtcgcgc ggctccctgc ggcgctccgc ggcgagggcg tggccgtact
     1201 cgaagagctc gagcgccgcc gggctccgga agggcacgag cctgtacgcc tcgctctccg
     1261 ggctgtcgtg gaggacgtcc gcgtagtcgg gatccgtgtt cgcgatcatc cggtcgcccc
     1321 agtcgatcag gcggtcggtg tccgattcgg gcgtgccgag cagccgtgcg agaacgcgga
     1381 tcggaagctc cttggcgatc tcctcgacga agtcgaactc cccgagcggg agcgcccggt
     1441 cgaggatctc gcgcgtgagc tcccgcagcg cgagctcgta cgccttgacc gcgcgcggcg
     1501 tgaacggcgg gccgacgagg ccgcgcaggc gcgtgtggcg cggcggatcc gtctcgagca
     1561 tcgaccggcg cgccgccatc atctcctcgt cgaggtcctc gaggttcgcg ccctgcgccg
     1621 aggagaacgt cttcgtgtcc cgcgagaccg agacgacgtc cgcgtgcttc gtcacgcacc
     1681 agaacccgcg cccgcccggc cagtcgtacc accagaccgg cgcctcgcgg cgcaggaggg
     1741 cgaaggtctc gtgcgggatc cgctcgacga acgcgtcgtt gccgaggtcg atctcgtcca
     1801 gggccgggga ggctgccatt cgtgcaagta tccgtccgcg tgcaagagga gagcgtggag
     1861 agcaggcgtg cgcgcgggct cgcgcggctc gcggagctcg gggacgagcc gggcggcgag
     1921 gagttcctcg cacgcctcgg cgatctcggc gacctgaccg tcgacttcgt cttcggcgac
     1981 gtgcacgcgc gtcccgggct cggggtgcgc gagcgggagc tcgtgatcgt cgccgtcctc
     2041 accgcgctcg gcgggctgga cccccaggtg cgcgcgcaca tccgcgccgc acgcgaggcc
     2101 ggcgtgagcg aagaggagct ggaggagacg atgatccagg tggcgccgta cgcgggcttc
     2161 ccgcgcgcga tcaacgggct gaaggtcctg cgcgaggagc agggccggtg agggcggcgc
     2221 ggttcgtcga cccgtccggc gcggagcgga tcggccgcta cgcggacgag ggcgtcgtcg
     2281 acgcggggcc ggccggtgtc ggcgggttcg tgccgtcggc cgagggctgg gacgaggtgg
     2341 aggccgcctc cggcccggag tacgcgctcg acgaggtccg gctcctgcac ccggtcgtgc
     2401 cggcgaagct cgtcgggatc gggctgaact accgcgagca cgcggccgag tccgagctcg
     2461 acgtcccgga ggtgccggtc gcgttcgcca tctggccgtc cgcgctcgtc ggcccgggcg
     2521 cgccgatcgt cgtcccgcgc gacgagacgc ggccggacta cgagggcgag gtggcgatcg
     2581 tcctcggccg cacggtgaag aacgtctcgc ccgaggaggg gcttgccgcg atcggcggct
     2641 tcgcgccgtt caacgacgtg tccggccgcc gggcgcagct cgagaccccc ctccggcagt
     2701 tcacgatcgg gaagagcttc gacacgttct ccccgatggg gccgtgcgtc gcgtccgcgg
     2761 acggcgtgga cctcgacgcg ctcgacgtgc ggacgacggt gagcggcgag gttctccagg
     2821 aggcgagcac gcgcgacctc atcttcgact tcggccggat cgtctcgtac ctctcgaccg
     2881 ggatcacgct cgaggcggga acggtggtcg cgaccggcac gcccggcggc gtcggcgaca
     2941 cgcgcgaccc gaggcgctac ctcgaggagg gcgacgtggt cgaggtcttc gtctccggcg
     3001 cgggcaccct cacgaacccg gtcgagcgcg agcgctagcc cgcgcagcta gcctgggcgc
     3061 gtggcctcag cctctcgcag ccggcgccgg atcgcggtcc tcgtgccgct cgcgcttctc
     3121 agcgcgttcg tcgtcgccgg ggtcgtcgtt cgctggcgct acacgggctc gcacgaggca
     3181 gcgaacctgc cgtggaacct gttcctcgcc tggatcccgc tcggcctcgc gctcgcggtc
     3241 cacgacgcgc accgtggggg cgcccggctc gcgtggcttc tcgccggcgg cggcctctgg
     3301 ctcctcttct tcccgaacgc cccctacctc gtgaccgatc tcaagcacct cggggcctgg
     3361 tcgggcgcgc cggagtggtt cgacgtcgtc ctcgtctccg ccgccgcctg gacggggctc
     3421 ctgctcggct tcgcctcgct ctacctcgtc caggccgtcg tgcgcgagct gcgcggcgcg
     3481 gccgcggcgt gggccgtcgt cgtcggcagc atcgcgctcg gctcgttcgg gatctatctc
     3541 gggcggttcg agcgctggaa ctcctgggac gcgctgacga gcccgcgccc catcctggcc
     3601 gacctctggg cgggagccat gaatccggtc gcgcacccgc gcgtgctcgc ggtcaccgtg
     3661 ctgttcacgg gcttcctgac gctcgcgtac ctgacgttct acggcctggt cgcgctcggc
     3721 cggaacgagc gggagccgct ctgatggagc agcgcgtcag cctcgtcacg ctcggcgtcc
     3781 acgacctcgg gcgggcgcgg cggttctacg aggcgctcgg ctggacgacg ggcgcggcgc
     3841 ccgcggacga cgtcgccttc ttccaggcgg gcgggatggt ggtcgcgctc tggggccggg
     3901 aggagctcgc agccgacagc ggcgtcgagg acgcgggcgg ctggggcggt gtgacgctcg
     3961 cgcacaacgt ccgctcgccg gccgacgtgg atgccgtcgt cgaggaggcc cgcgccgccg
     4021 gcgcggcgat cacgcgcgag ccggccgaga cgttctgggg cggctactcg ggcgcgttca
     4081 ccgacccgga cggccacgcg tgggagatcg cccacaaccc gagctggacc ctcgcggacg
     4141 acggctccgt gcggcttccc gcctgagccc gttctccggc caaagcccca ccctgggagg
     4201 ggacaggggc caccacccgc cgcccactcg gaggctacgc ctccagggcc tcggcggcag
     4261 cgggcggcgt gtgccaactt cggcacgaac ctgcgccgat tcccgcacac gagcgggttc
     4321 tgccggagag cccgccggcg ctagggcgcg agcccggcgt cgatccaggc ccggaagcgt
     4381 gcgatctgct cctccggcca ctcgccgtcg cacggcatcg agccgtcctc gagccgctcg
     4441 aggatcgcgg cggcgttgtc gcgcacgtcc ccgtactcga agaggtcgaa cgcgaactcc
     4501 atcgcctccc tgtcgtcctc gcggaagagc gggcggatgt ccgccgcgaa cgagggcccc
     4561 gagccgttcc ccgcgctcac agcgtcacgg cgtactcgtt gagctcccag tcggtgacgg
     4621 ccgacgcgaa gcgcgccacc tcgctccgct tcatggcgag gaacgcctgc acgaactgcg
     4681 gcccgaggtg ctcgacgaag aacgtgtcct cctcgagcgc gtcgagcgcc tctccgaggg
     4741 agaagggcat gagcgtccgg tcctcgagct cgtaggcgac gccgctcacc ggctcgcccg
     4801 ggtcgagctc gcgctcgagc ccgtcgatgc ccgcgaagag ggccgccgcg gaggcgatgt
     4861 aggcgttcgc ggtcccgtcg ccggcgcggt tctcgatccg cgtcgccttg ccccgctcgc
     4921 gcgggatgcg cgcgtagacc gtccggttgt cgtggcccca cgtcacgttc cacgggccga
     4981 acgccatggc gaggaaccgc ttgtacgcgt tgatcgtcgg cgccatgatc gccgtcatcc
     5041 cccgcgcgtg ctcgagctgg cccgcgagga actgccggca gacctcggag agcccgtccg
     5101 gcccgtcggg gtcgtcgaag gcgttcgtgc cgtcgtccca gagcgacagg tggaggtggt
     5161 acccggacgt cccgagctcc tggatcggct tccccatgaa cgtcgccacg agcccgtgct
     5221 gcgccgcgat ctccttcacg gcgagcttca ggaggtgcgc gtcgtccgcg gccttgagcg
     5281 cgtcgtcgta gcggaggttg atctcccact gcgagccgaa gtactcctgg ttcgaggcga
     5341 tgagcgggag ccccatcgcc gcgacggcgt cctccatctt gcgcaggacg ccctcggtgt
     5401 cgacgctggg cgagagcgtg tagacgaggc cgggctgctt cgcgtactgc tcgtagcccg
     5461 agccgttcgg gcggaggagg aagaactcga gctcgtggcc cgcgacggcg cggaagccgc
     5521 gcttcccgag gtcgtccgag gcgcgcgcga ggagctggcg cggcgacgcg ggcagccagt
     5581 cgccgtcgaa gcgcacgtcg ccgatcgcga tcgcggtcga gcgctcccac ggcacgacgg
     5641 tgagcgtgtc gaggtcgggg atcacctggg cgtcccggaa gtcgacctcc gcgccgagcc
     5701 cggttccctc gatcacctcg ccgacgtggt cgacgcagaa cgccgagatg cagaagttga
     5761 tcccgtcctc gaggcactcg tcgagctgcg tgagcgggac gaccttgttc cgggagacgc
     5821 cgagcaggtc ggtgaaatgg acgcgcaggc ggtcgcagcc cgcctcgcgg acgtgcgcga
     5881 gccgctcctc ggccgagagt cgctccccca tcgtggccct aatcgtgccg gatgtgaggc
     5941 ccgccgggct ccactggcag cggcaggtcc tcctcgatca gctccgtctc gcggtagacc
     6001 tccgcgagcg gcacgcgccg gaactgcgac gtgagccccc cgcccatcgc attcatgagg
     6061 aaggacgccg ccgccgcgac ggcgaggaag atcccgtagt agaggtaggc gtcgttccgc
     6121 acctcctcga ccgggaagat cttgaacgcc gcgagcgcga tgagcccgga gccgacgagc
     6181 tgcgggatcg gtacgagcgg agcgcggaac ggccgcgcgg cgttcggctc cacgatccgg
     6241 aggatcacga ccgacaggtg gatgaggaag tacgcggtcg cccagccgag gacgccggcg
     6301 aggatgagct tgatgaagtt ctggctcgtc ccgaggtcga cgacgacgtt catgaggatc
     6361 gggagcgacg cgatcacgag gatcccgacg acgggcgcgc gcgtcgacgg caggaggtag
     6421 gcgaacacgg aggggagctg gcgcgtcagg cccatcccgt agaggatccg cgggatcccg
     6481 gcgatgagcg cgttcagcgt cgagagcgtc gcggcgatcg agacgaccgc catccaccac
     6541 ttgcccgtgt cgccgaggat cgcgatcgcc gtctcgatgt gcgcgacggg gctgaaggcc
     6601 tcgagctgct cggcgggcac gtagcgcgcg gccgcgagcc cgaagagcac gaccgggacg
     6661 ctcacgagcg tgaggccgag gaagatcccg agcgggatgt tgcggccggg attctccatc
     6721 tcctccgcga gcgggcacgt gtactccatc ccgacgaagg tgaagagcgc gatcccgagc
     6781 agcgtcgaga agaggctcca cgtgaagggc acgtccggga agagtccgcc gatcgggtcg
     6841 gtcgtcccga ggccgagcac gccgatcagc ccgaagatga gcagcgagag cgcgacgaag
     6901 gcggtcaggc cgacctcgac cgcgccgtac gggcggatcc ccgcgatgtt gaggagcgcg
     6961 acgaagacga cgaagacgat cacccagacc gtcgtcgagg ggccgtccgt ccagaggaag
     7021 cccttgatga tcgcggacgg cgtgtacgac tccgcggcgg tcgggaagag gatcaccgcg
     7081 atgtagccca cgagcacggt gagggtcgcg aagaacggcc cgagcccggc gcgcacgtac
     7141 tcgttcatcg agccggcctt cgggatcatc gtggcgagct ccgcgaagct catcgactgg
     7201 aggtacatcg cgagcagccc gaccgcgagc gcggcgagga acacgtagct cgcgaagaac
     7261 ccggccgtga ccgtcgcgag gacggtcgag gcgacgatca ggccgaacgt cgtcccgatc
     7321 gccgtgggca ggcccaccgc ccgccgctcg agccccccgt ttccgccctg gtctgcacgc
     7381 gccgccatgc cgcctccctc gctcgccgtc gccgaccgcg cgagtatcca ccgtcgcgag
     7441 gacggcgtgc aacccgccgg gggctaggtc accgagcggc tgaagcgcgg gccgagcgtg
     7501 gcgatgagca cggcggcgac cgcga
//
LOCUS       DASTFC010000365         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_194081,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000365 DASTFC010000000
VERSION     DASTFC010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194081"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..196
                     /locus_tag="VFO88_10760"
     CDS             44..196
                     /locus_tag="VFO88_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546044.1"
                     /translation="MDTVLGLLGILVWIVTVIALASAVTYVVVKLFPADKPEKDEPTA
                     DGANAS"
     gene            complement(193..1317)
                     /gene="ald"
                     /locus_tag="VFO88_10765"
     CDS             complement(193..1317)
                     /gene="ald"
                     /locus_tag="VFO88_10765"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9546045.1"
                     /translation="MRIGVVKEIKPDEYRVALTPAGARELAQRGHEVYVEAGAGEGSA
                     FPDGEYAAVGARLASADEVWEAVDLLLKVKEPIAEEYPRLREGQVLFTYLHLAADEPL
                     TRALMESGTTAIAYETVETADHQLPLLAPMSEVAGRLAAQAGAHFLEKPLGGRGLLLG
                     GVAGVLPGKVAILGGGMVGYNSAVIALGLGAQVRILDKSVNRLRQLEQVLSGRVELVM
                     SSALEVERAVLDADVVIGAVLIPGAKAPKLVSAAMVKEMKRGAVLCDVSIDQGGCFET
                     SHPTTHSDPVYVVDNVQHYCVSNMPGAVPITSTVALTNVTLPYIESIADVGVREAVAS
                     DPSLALGVNVIDGKVTYEAVAEAHGLEATPLSEVLPLEPV"
     gene            complement(1335..1838)
                     /locus_tag="VFO88_10770"
     CDS             complement(1335..1838)
                     /locus_tag="VFO88_10770"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9546046.1"
                     /translation="MREPDRSERVYEGDVLGVTVELWDGAKREIVERADAVAIVAADA
                     EDRLVLVRQPREAARAELVELPAGTIDGDEEPLATARRELREETGLHGGRWRAGPAVY
                     STPGFCRERMHLFLADGLQEGDPDPDAGERIEVVRWDAAEVEERLAAIADAKTLAGML
                     LFLRERA"
     gene            complement(1831..>2061)
                     /locus_tag="VFO88_10775"
     CDS             complement(1831..>2061)
                     /locus_tag="VFO88_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546047.1"
                     /translation="PVRLAFEALAACGFEPRGVRAGGGADGNVFTARGRPCVNIANGM
                     TRIHSADEHIAVADLDAMVDVTLALVDAARRA"
BASE COUNT          275 a          775 c          726 g          285 t
ORIGIN      
        1 atgacgcagg cgcgcggcat ggtgccgcta ttctagtgcc cgcatggaca ccgtcctcgg
       61 cctcctcggc atcctcgtct ggatcgtcac cgtgatcgcg ctcgcctcgg cggtcacgta
      121 cgtcgtcgtg aagctcttcc cggccgacaa gccggagaag gacgagccga cggcggacgg
      181 cgcgaacgcg tcctagaccg gctcgagcgg gaggacctcg gagaggggcg tcgcctcgag
      241 cccgtgcgcc tcggcgaccg cctcgtacgt gaccttgccg tcgatcacgt tcacgccgag
      301 ggcgagcgag gggtcggacg ccaccgcttc ccgcacgccg acgtccgcga tcgactcgat
      361 gtaggggagc gtcacgttcg tcagggcgac ggtcgaggtg atcggcacgg cgcccggcat
      421 gttcgacacg cagtagtgct gcacgttgtc gacgacgtag acggggtcgg aatgggtcgt
      481 cgggtgcgag gtctcgaagc acccgccctg gtcgatcgag acgtcgcaga ggactgcccc
      541 gcgcttcatc tccttgacca tggcggccga gacgagcttc ggcgccttcg ctcccgggat
      601 gagcacggca ccgatcacga cgtcggcgtc gagcaccgcg cgctcgacct cgagcgcgct
      661 cgacatcacg agctcgaccc gtcccgagag gacctgctcg agctgacgga gccggttgac
      721 cgacttgtcg aggatccgca cctgggcgcc gagcccgagc gcgatcacgg cggagttgta
      781 gccgaccatc ccgccgccga ggatcgcgac cttgcccggt aggacgccgg cgaccccgcc
      841 gagcaggagc ccgcgcccgc cgagcggctt ctcgaggaaa tgcgccccgg cctgcgcggc
      901 gagccggccc gcgacctcgc tcatcggcgc gagaaggggt agctggtggt cggccgtctc
      961 caccgtctcg tacgcgatcg cggtcgtgcc gctctccatg agggcgcgcg tgagcggctc
     1021 gtcggcggcg aggtggaggt acgtgaagag cacctggccc tcgcggagcc gcgggtactc
     1081 ctccgcgatc ggctccttga ccttgagcag gaggtcgacc gcctcccaga cctcgtccgc
     1141 gctcgcgagc cgcgccccga ccgccgcgta ctccccgtcc gggaacgcgc tgccctcgcc
     1201 ggcgcccgcc tcgacgtaga cctcgtgccc gcgctgcgcg agctcgcgcg cgccggccgg
     1261 cgtcagggcg acacggtact cgtccggctt gatctccttg acgaccccga tccgcatggc
     1321 gcgaaacgat atcgctacgc gcgctcgcgg agaaagagga gcatccccgc gagcgtcttc
     1381 gcgtccgcga tcgcggcgag ccgctcctcg acctcggccg cgtcccagcg gacgacctcg
     1441 atccgctccc cggcgtccgg gtcggggtcg ccctcctgga gcccgtcggc gaggaacagg
     1501 tgcatgcgct cgcggcagaa gccgggcgtc gagtagacgg ccgggccggc gcgccagcgg
     1561 ccgccgtgca gcccggtctc ctcccggagc tcgcgcctcg ccgtcgcgag cggctcctcg
     1621 tcgccgtcga tcgtcccggc cggaagctcg acgagctccg cgcgggcagc ctcgcggggc
     1681 tggcgcacga gcaccaggcg gtcctccgcg tcggcggcga cgatcgcgac cgcgtccgcc
     1741 cgctcgacga tctcccgctt cgcgccgtcc cagagctcga ccgtcacgcc gaggacgtcg
     1801 ccctcgtaca cgcgctcgct ccgatccggc tcacgcacgg cgggcggcgt ccacgagcgc
     1861 gagcgtgacg tcgaccatgg cgtcgaggtc ggcgacggcg atgtgctcgt cggcgctgtg
     1921 gatccgggtc atgccgttcg cgatgttcac gcacgggcgg ccgcgggcgg tgaacacgtt
     1981 gccgtccgcg ccgccgcccg cgcgcacgcc gcgcggctcg aagccgcacg cggcgagcgc
     2041 ctcgaacgcg agccgcaccg g
//
LOCUS       DASTFC010000366         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_375092,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000366 DASTFC010000000
VERSION     DASTFC010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375092"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFO88_10780"
     CDS             complement(<1..377)
                     /locus_tag="VFO88_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HET9546048.1"
                     /translation="MSDRPHVLLVDNYDSFTYNLAHLFEELGAEVTVLRNDAVDADAA
                     EALGPTHLVISPGPGRPEGAGATLEIVRRLGPRVPTLGVCLGHQAIVEAYGGTVGQAQ
                     RLVHGKASRVAHSGEGVFAGLPDP"
     gene            complement(374..1618)
                     /locus_tag="VFO88_10785"
     CDS             complement(374..1618)
                     /locus_tag="VFO88_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET9546049.1"
                     /translation="MTVSSAIQTDLVTPLGAYLALRDTGRASFLLESVERGRLGRHSL
                     VGAGSRLVTFEEAEREVAAGSPVVGFLGYDHVAVLEPTVPLPEPGPGLPDSRFVVAET
                     LVRFDHAAAHADVLAGDRDAVAAALARPVQAPEGEGPLPRGETRRSPDQAAYEESVRE
                     AKRLIAAGDAFQVVLSQRAERPTDVSPVRLYRALRQVNPSPYLFLLELGDDLALVGSS
                     PETLVKLTGRRASLNPIAGTTPAGDGDAERLLASEKDRAEHVMLVDLGRNDLSRVCRA
                     GTVRVERFLEAERYSHVTHLVSEVAGELADGRTAFDLLRACFPAGTVSGAPKVRAMQI
                     VSELEGVRRGPYAGAVGYALPDGDLDTCIAIRTVVLTPGLARLQAGAGIVADSDPEAE
                     HLECLSKLAALEAALDLAEEGP"
     gene            complement(1795..2535)
                     /locus_tag="VFO88_10790"
     CDS             complement(1795..2535)
                     /locus_tag="VFO88_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9546050.1"
                     /translation="MRLLAAEVLKLRTTWGFWVYLAMLLVLTALVVVGTIGSGVGAFD
                     DQSWRDLLQAPLIGFAFPLLVGSVVVTSEFRHGTIGQSLLVTPRRPVLLAAKLAVAGL
                     VGLVFAVLSFALMLAIALPWLGAKDVDVGLGDPIVRETALVVTVAFVLLGMLGVGVGG
                     ALRNQAGAAVGLLVWFLVAEFLVRALLGVIELDGVARYLPLAAMEALIGSDDLEDVLS
                     QSAGGLVLAGWVAVAAAAATGSLRRDVT"
     gene            complement(2532..>3153)
                     /locus_tag="VFO88_10795"
     CDS             complement(2532..>3153)
                     /locus_tag="VFO88_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9546051.1"
                     /translation="NHLRTVATAAGIPRGRVEELLELVGLTAAAKRRVGGYSLGMRQR
                     LGLAAALLGDPQVLVLDEPANGLDPEGIRWLRNFLRRLADEGRTILVSSHVLAELAQT
                     VDDVAIIARGRLVAHAPVETLTARAGGRTRVRSPQAEALREALAADGIEGASLEDGAL
                     AVSAPPERVGELAAANGIVLHELRTESSSLEEIFLQLTGGDAAVPR"
BASE COUNT          445 a         1151 c         1213 g          344 t
ORIGIN      
        1 gggtcgggca acccggcgaa cacgccctcg ccgctgtggg cgacccggct cgccttcccg
       61 tgcacgagcc gctgcgcctg cccgacggtg ccgccgtatg cctcgacgat cgcctggtgg
      121 ccgaggcaga cgccgagcgt cggcacgcgc gggccgagcc ggcggacgat ctcgagcgtc
      181 gcgcccgcgc cctcgggacg gccggggccg ggggagatga cgaggtgggt cggcccgagc
      241 gcctcggcag cgtccgcgtc gaccgcgtcg ttgcgcagca cggtcacctc ggcgccgagc
      301 tcctcgaaca ggtgcgcgag gttgtacgtg aacgagtcgt agttgtcgac gagcaagacg
      361 tgcggcctgt cgctcacggc ccctcctcgg cgaggtcgag ggcggcctcg agcgcggcga
      421 gcttcgagag gcactcgagg tgctcggcct ctgggtccga gtcggcgacg atcccggcgc
      481 cggcctggag ccgcgcgagg ccgggcgtga ggacgaccgt ccggatcgcg atgcaggtgt
      541 cgaggtcgcc gtccgggagc gcgtagccga ccgcgcccgc ataggggccg cggcgcacgc
      601 cctcgagctc cgagacgatc tgcatcgccc gcaccttggg cgcgccagag acggtcccgg
      661 ccgggaagca ggcgcggagg aggtcgaacg cggtgcgccc gtccgcgagc tcgcccgcca
      721 cctccgagac gaggtgcgtc acgtgggagt accgctcggc ctcgaggaag cgctcgaccc
      781 gcacggtgcc cgcgcggcag acgcgcgaga ggtcgttccg gccgaggtcg acgagcatga
      841 cgtgctcggc gcggtccttc tcggaggcga gcagccgctc ggcgtcgccg tcgcctgcgg
      901 gcgtcgtccc cgcgatcggg ttgaggctcg cgcggcggcc ggtcagcttg acgagcgtct
      961 ccggggagga gccgacgagc gcgaggtcgt cgcccagctc gaggaggaag aggtacggcg
     1021 aggggttcac ctggcggagc gcgcggtaga gccgcacagg ggagacgtcg gtcggccgct
     1081 cggcgcgctg cgagaggacg acctgaaacg cgtcccccgc cgcgatcagc cgcttcgcct
     1141 cgcggacgct ctcctcgtac gcggcctggt cgggcgagcg ccgcgtctcc ccgcggggaa
     1201 gcgggccctc gccctccggg gcctgcacgg gccgcgcgag cgccgccgcg accgcgtccc
     1261 ggtcgcccgc gaggacgtcc gcgtgcgcgg cggcgtggtc gaagcgcacg agtgtctcgg
     1321 cgacgacgaa ccggctatcc ggcaggcccg gccccggctc cggcagaggc acggtcggct
     1381 cgagcacggc cacgtggtcg tagcccagga acccgaccac gggggagccg gccgcgacct
     1441 cgcgctcggc ctcctcgaac gtcacgagcc gcgacccggc accgacgagc gagtgccgcc
     1501 cgagccgccc gcgctcgacc gactcgagga ggaagctcgc gcgtccggtg tcgcgcagcg
     1561 ccaggtacgc cccgagcggg gtgacgaggt cggtctggat cgcgctggag acggtcatcg
     1621 gcaacaaaaa accttccggg tggaaggtcg agtcgagcgg tcgggctggt cgcgcctgcg
     1681 tctcagcagg cggcgctccc gcaccgctcg acgggccagg cccagaggat gtgggagggg
     1741 gaggtcacgg tgctcggagg gtacgccgcg tccgcggcgc ggtcaagcgg cttcctacgt
     1801 cacgtcccgg cggaggctgc ccgtggcggc cgcggccgcg acggccaccc agccggcgag
     1861 cacgagcccg ccggcggatt gcgagaggac gtcctcgagg tcgtcgcttc cgatcagcgc
     1921 ctccatcgcc gcgagcggca ggtagcgcgc gacgccgtcg agctcgatga cgccgagaag
     1981 cgcgcgcacg aggaactcgg ccacgaggaa ccagacgagc agcccgaccg ccgcgcccgc
     2041 ctggttgcga agcgccccgc cgaccccgac gccgagcatc ccgagcagca cgaacgcgac
     2101 cgtgacgacg agcgccgtct cccgcacgat cgggtcgccc agcccgacgt cgacgtcctt
     2161 cgcgccgagc cacgggagcg cgatcgcgag catgagcgcg aacgagagca cggcgaagac
     2221 gagcccgacg aggccggcca cggcgagctt cgccgcgagg aggacggggc ggcgcggggt
     2281 gacgagcagg ctctggccga tcgtcccgtg ccggaactca ctcgtgacga cgaccgaccc
     2341 gacgaggagc gggaacgcga acccgatgag cggcgcctgc aggaggtcgc gccacgactg
     2401 gtcgtcgaag gccccgaccc cggagccgat cgtccccacg acgacgagcg cggtcagcac
     2461 gaggagcatc gcgagataga cccagaagcc ccacgtcgtg cggagcttca gcacctcggc
     2521 cgcgaggagc ctcaccgcgg caccgccgcg tcgccgccgg tcagctggag gaagatctcc
     2581 tcgagcgagg aggactcggt gcgcagctcg tggaggacga tcccgttcgc ggccgcgagc
     2641 tcgcccacgc gctcgggcgg ggcggagacg gcgagcgcgc cgtcctcgag cgaggctccc
     2701 tcgatcccgt ccgcggcgag cgcctcgcgg agcgcctcgg cctgcgggga gcggacgcgc
     2761 gtgcgcccgc ccgctcgcgc ggtcagcgtc tcgacgggcg cgtgcgcgac gaggcggccg
     2821 cgcgcgatga tcgccacgtc gtcaacggtc tgcgcgagct cggcgaggac gtggctcgag
     2881 acgaggatcg tgcggccctc gtcggccagc cggcggagga agttccggag ccagcggatc
     2941 ccctccgggt cgagcccgtt cgcgggctcg tcgaggacga gcacctgcgg gtcgccgagg
     3001 agcgccgccg caagcccgag ccgctgccgc atcccgagcg agtagccgcc cacgcgccgc
     3061 ttcgccgcgg ccgtgaggcc gacgagctcg agcagctcct cgacgcgccc gcgcgggatg
     3121 ccggccgccg tcgcgaccgt ccgcaggtgg ttg
//
LOCUS       DASTFC010000367         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_655244,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000367 DASTFC010000000
VERSION     DASTFC010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655244"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFO88_10800"
     CDS             complement(<1..596)
                     /locus_tag="VFO88_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9546052.1"
                     /translation="MDVLTPRTLDEALRLRAETADAWPIQGGTDVMVELNFDRARPRV
                     VLNLNEVAELRGFARENGTLRLGAGLTYTECRRELGETLPALAEAARTVGSPQIQNRG
                     TIGGNLGTASPAGDALPPLLVEEAEVELAGPAGRRTLALEEFLVGPKRNALAEDELIV
                     AVHVRPSGARQTFMKVGPRNAMVIAVASLALAVDRERG"
     gene            626..2326
                     /gene="ade"
                     /locus_tag="VFO88_10805"
     CDS             626..2326
                     /gene="ade"
                     /locus_tag="VFO88_10805"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HET9546053.1"
                     /translation="MEELSRRLAVARGDEPADVVVRGGRVLCVFTREWLDADVAVADG
                     YVAGLGDYEGREEVDASGRYVVPGFVDAHMHLESAKLLVDEFARLVLPLGTTAVVADP
                     HEIANVLGTDGVHWLADLCHDLPLDVYFMASSCVPASEFESPRRALLPGDLEGLLRRR
                     RVLGLAEMMNFPGVVAGDERELEKLRLDGATHVDGHAPGVTGKALNAYAAAGIRSDHE
                     AITLDEGRERLRAGMWLLIREATGARNLAALAPLLTEYGPGRIAFCTDDREPEHIADD
                     GHVNSMVRDAVAAGVAPEDALVAATLSPSTWHGLAEHGAVAPGYRADLVLLPDLEEFR
                     PELVLKRGAPVGEIRRPDVPEWVKHTVRVGDVAGERFSLPAPASGRVRVIGVVRDQIV
                     TESLVEDAPLADGHVAADPARDLAKIAVIERHLGTGRVGLGLVRGFGLERGALASTVA
                     HDAHNIVVVGMSDEDMAAAVARLRELGGGVVVVDDGELRAELPLPVAGLLSTARLDEV
                     VAASRACVEAAHALGCELHSPFQTLAFLALSVIPRLKITDRGLVDVDAFRLVPLEAGA
                     "
     gene            2323..>2884
                     /locus_tag="VFO88_10810"
     CDS             2323..>2884
                     /locus_tag="VFO88_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9546054.1"
                     /translation="MSLLLTGGYVVTMDDAGTEHENGWVLVEDGFVQAVGDGPPPKAE
                     RVERLNGALVTPGLVNTHHHLYQTLTRTRAQEADLFTWLKTLYPLWGRIDAESEYAAA
                     RTGLAELALSGCSTVFDHHYVFPPGREGIVEAEVQAARELGVRIVASRGSMDLGESEG
                     GLPPDSLVEDGDAALAATEALAATLHE"
BASE COUNT          350 a         1086 c         1067 g          381 t
ORIGIN      
        1 tcgccgcgct cccggtcgac cgcgagcgcg agcgaggcga cggcgatcac catcgcgttc
       61 cgcggcccca ccttcatgaa cgtctgccgc gccccggacg ggcgcacgtg gacggcgacg
      121 atcagctcgt cctcggcgag cgcgttgcgc ttcggcccga cgaggaactc ctcgagcgcg
      181 agcgtgcggc ggccggccgg gcccgcgagc tcgacctccg cctcctcgac gaggagcggc
      241 gggagcgcgt ccccggcggg cgaggccgtg ccgaggttcc cgccgatcgt cccgcggttc
      301 tggatctgcg gcgagccgac cgtgcgcgcg gcctcggcga gcgccggaag cgtctcgccg
      361 agctctcgcc ggcactccgt gtacgtgagc ccggcaccga gcctgagcgt gccgttctcg
      421 cgcgcgaagc cgcgcaactc ggcgacctcg ttcaggttca ggacgacgcg cggacgggcc
      481 cggtcgaagt tcagctcgac catgacgtcg gtgccgccct ggatcggcca cgcgtccgcc
      541 gtctccgccc ggagccgcag cgcctcgtcg agcgtccgtg gggtgagcac gtccacgcgg
      601 cccaagggta gtacgttacg gcggcatgga ggagctgtcc cgccgactgg cagtggcacg
      661 cggggacgag ccggcggacg tggtcgtccg cggcggccgt gtcctctgcg tcttcacgcg
      721 ggagtggctc gacgcggacg tggccgtcgc ggacgggtac gtcgccggcc tcggcgacta
      781 cgagggccgc gaggaggtcg acgcgagcgg ccgctacgtc gtgccgggct tcgtcgacgc
      841 gcacatgcac ctcgagtcgg cgaagctcct cgtggacgag ttcgcgcggc tcgtgctccc
      901 gctcggcacg accgccgtcg tcgcggaccc gcacgagatc gcgaacgtgc tcggcacgga
      961 cggcgtccac tggctcgccg acctctgcca cgacctgccg ctcgacgtct acttcatggc
     1021 ctcgtcgtgc gtccccgcgt cggagttcga gtcgccgcgg cgcgcgctcc tccccggcga
     1081 cctcgagggg ctgctccgcc ggcggcgcgt gctcgggctc gcggagatga tgaacttccc
     1141 cggcgtcgtc gcgggcgacg agcgcgagct cgagaagctc cggctcgacg gcgcgacgca
     1201 cgtggacggg catgcgccgg gggtcacggg gaaggccctg aacgcgtacg ccgcggccgg
     1261 gatccgctcc gaccacgagg cgatcacgct cgacgagggg cgcgagcggc tccgggcggg
     1321 gatgtggctc ctgatccgcg aggcgaccgg cgcgcggaac ctcgccgcgc tcgcgccgct
     1381 cctcacggag tacgggcctg ggcgcatcgc cttctgcacc gacgaccgcg agccggagca
     1441 catcgcagac gacggccacg tcaattcgat ggtgcgcgac gcggtcgccg caggcgtggc
     1501 cccggaggac gcgctcgtcg cggccacgct cagcccctcg acgtggcacg gcctcgccga
     1561 gcacggtgcg gtcgcgcccg gctaccgggc cgacctcgtc ctcctcccgg acctggagga
     1621 gttccggccc gaactcgtgc tgaagcgcgg cgcgccggtc ggcgagatcc ggcggccgga
     1681 cgtgccggag tgggtgaagc acaccgtgcg cgtcggcgac gtcgccggcg agcggttctc
     1741 gcttcccgcg cccgcgagcg gccgggtgcg cgtgatcggc gtcgtccgcg accagatcgt
     1801 gaccgagtcg ctcgtcgagg acgcgccgct cgccgacggg cacgtcgccg cagacccggc
     1861 gcgcgacctg gcgaagatcg ccgtcatcga gcgccatctc ggcaccggcc gcgtcgggct
     1921 cgggctcgtg cgcgggttcg gcctcgagcg cggcgcgctg gcctcgaccg tagcccacga
     1981 cgcgcacaac atcgtcgtcg tcggcatgtc cgacgaggac atggccgctg cggtcgcgcg
     2041 cctgcgggag ctgggcggcg gcgtcgtggt cgtggacgac ggcgagctgc gcgcggagct
     2101 gcccctcccc gtcgccgggc tcctctcgac cgcgcggctc gacgaggtcg tggcggcgag
     2161 ccgcgcctgc gtcgaggcgg cgcacgcgct cggctgcgag ctgcactccc cgttccagac
     2221 gctcgcgttc ctcgcgctct ccgtcatccc gcggctcaag atcaccgacc gcgggctcgt
     2281 cgacgtcgac gcgttccggc tcgtcccgct cgaggcgggc gcgtgagcct cctcctcacc
     2341 ggcggctacg tcgtcacgat ggacgacgcc gggacggagc acgagaacgg ctgggtgctc
     2401 gtcgaggacg gcttcgtgca ggcggtaggc gacggcccgc cgcccaaggc cgagcgcgtc
     2461 gagcgcctga acggcgcgct cgtcaccccg gggctcgtga acacgcacca ccacctgtac
     2521 cagacgctca cgcgcacccg ggcgcaggag gccgacctct tcacgtggct caagacgctc
     2581 tatccgctct ggggccgaat cgacgccgag tccgagtacg cagcggcccg caccggcctc
     2641 gcggagctcg cgctttccgg ctgctcgacc gtcttcgacc accactacgt cttcccgccc
     2701 gggcgcgagg ggatcgtgga ggcggaggtg caggcggccc gcgagctcgg cgtgcggatc
     2761 gtcgcctccc gcgggtcgat ggacctcggc gagtcggagg gcgggctccc gccggactcg
     2821 ctcgtcgagg acggcgacgc cgcgctcgcc gccaccgagg cgctcgccgc gacgctccac
     2881 gagg
//
LOCUS       DASTFC010000368         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_95334,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000368 DASTFC010000000
VERSION     DASTFC010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95334"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..690
                     /locus_tag="VFO88_10815"
     CDS             67..690
                     /locus_tag="VFO88_10815"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET9546055.1"
                     /translation="MEISIIGTGNMARGIGTRALDGGHDVTFYGTTRENAQGLAGELS
                     RSAKAAAVGDPLEGDVVVLALWYPVTREVVERYGDALDGKVVVDISNPVNTETFERIV
                     PDAGSGAQEVAAAAPGARVVKAFNTTFAGTVMAGEVSGQPLDVLIASDDDEAKKTVAS
                     VAEDGGLRAVDAGPLERARELEAAGYLHMVVQEGLGTGYGSALRIVA"
     gene            738..1766
                     /locus_tag="VFO88_10820"
     CDS             738..1766
                     /locus_tag="VFO88_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET9546056.1"
                     /translation="MTRLTTTVVGSYPQPGWLIDRARLGDRLPPRVRARELWRVPEAF
                     LEEAQDDATRLAVQDMERAGIDVVTDGEMRRESYSNRFATALDGVDLDTPGRALDRTG
                     NPVPVPRVVGPVRRARPVEIRDVEFLRSITDRRIKITVPGPFTMTQQAQNDHYPSDRE
                     LALAYAEAVNEELRDLKAAGADVVQIDEPYLQARPDAAREYALEAIDRALDGIEGETV
                     LHTCFGYAALVHERPDGYPFLDELAACAATALSLEGAQPRLDPAILAPLAGKTILWGV
                     LDLGSEEVESPDEVAARIRAAVEVVGPERLVPAPDCGMKYLPRARAVAKLEALVAGAR
                     IVDAELGR"
     gene            1769..>2070
                     /locus_tag="VFO88_10825"
     CDS             1769..>2070
                     /locus_tag="VFO88_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9546057.1"
                     /translation="MYAHVERLLAEERAVILDGGVATELQRLRPLRGEPGPDPELWGT
                     WGLYRAPGAVKEVHARYVAAGCDVISTDTWSILSAPAADARAAVEPTHWMDVARLGV"
BASE COUNT          287 a          744 c          788 g          251 t
ORIGIN      
        1 gcgttttcgg ccccgccgag cggaaaggcc gaaaggcaga gcaggcgcac gcgaggagga
       61 gattccatgg agatctcgat catcgggacc ggcaacatgg cgcgcggcat cggcacgcgc
      121 gcgctcgacg gggggcacga cgtcacgttc tacggcacga cgcgcgagaa cgcgcagggg
      181 ctggccggcg agctgagccg gtcggcgaag gcggccgcgg tcggcgatcc gctcgagggc
      241 gacgtcgtcg tgctcgcgct ctggtatccg gtcacgcgcg aggtcgtcga gcgctacggc
      301 gacgcgctgg acgggaaggt cgtcgtcgac atctcgaacc cggtgaacac ggagacgttc
      361 gagcggatcg tccccgacgc gggctccggc gcgcaggagg tcgccgccgc ggcgcccggc
      421 gcgcgcgtcg tgaaggcgtt caacacgacg ttcgccggaa cggtcatggc gggcgaggtg
      481 tcggggcagc cgctcgacgt tctgatcgcg tccgacgacg acgaggcgaa gaagaccgtc
      541 gcgagcgtgg ccgaggacgg cggcctgcgg gccgtcgacg cgggcccgct cgagcgggcg
      601 cgggagctcg aggcggcggg ctacctccac atggtcgtcc aggagggcct cggcaccggc
      661 tacgggagcg cgctcaggat cgtcgcctga cgctgtaacc cgatcgcagt cgggaccggc
      721 gcgatacgct cgccgcgatg acgcggctca ccacgaccgt cgtcgggagc tatccccagc
      781 cgggctggct gatcgaccgc gcccggctcg gagatcggct ccccccgcgc gtgcgtgcgc
      841 gcgagctctg gcgcgtgccg gaggcgttcc tcgaggaggc gcaggacgac gcgacgcggc
      901 tcgcggtcca ggacatggaa cgcgccggga tcgacgtggt cacggacggc gagatgcgcc
      961 gcgagagcta ctcgaaccgc ttcgcgaccg cgctcgacgg cgtcgacctc gacacgcccg
     1021 gccgggcgct cgaccgcacc gggaacccgg tgcccgtccc gcgcgtcgtc gggccggtga
     1081 ggcgcgcgcg gccggtcgag atccgcgacg tcgaatttct gcgctctatc accgaccggc
     1141 ggatcaagat caccgtcccg ggcccgttca cgatgaccca gcaggcgcag aacgaccact
     1201 acccgtcgga tcgcgagctc gcgctcgcgt acgcggaggc cgtgaacgag gagctccgcg
     1261 acctgaaggc ggccggggcg gacgtcgtcc agatcgacga gccatacctc caggcgcggc
     1321 ccgacgcggc gcgcgagtac gcgctcgagg cgatcgaccg ggcgctcgac gggatcgagg
     1381 gcgagacggt gctccacacg tgcttcggct acgccgcgct cgtgcacgag cggccggacg
     1441 gctacccgtt cctcgacgag ctcgccgcgt gcgccgcgac cgcgctctcg ctcgagggcg
     1501 cgcagccgag gctcgacccg gcgatcctcg cccccctcgc gggcaagacg attctctggg
     1561 gcgtcctcga cctcggctcg gaggaggtcg agtcgccgga cgaggtcgcc gcccgcatcc
     1621 gcgccgccgt ggaggtcgtg gggcccgagc ggctcgtccc ggcgccggac tgcgggatga
     1681 agtacctccc gcgcgcccgc gcggtcgcga agctcgaggc gctcgtcgcc ggcgcgcgca
     1741 tcgtggacgc cgagctcggc cgctagcggt gtacgcccac gtcgagaggc tgctcgccga
     1801 ggagcgcgcg gtcatcctgg acggcggcgt cgcgaccgag ctccagcggc ttcgcccgct
     1861 gcgcggcgag ccgggcccgg accccgagct ctggggcacg tgggggctct accgcgcccc
     1921 cggcgccgtg aaggaggtgc acgcgcgcta cgtcgctgcc ggctgcgacg tgatctcgac
     1981 cgacacgtgg tcgatcctct ccgcgcccgc cgccgacgcg cgcgccgcgg tcgagccgac
     2041 gcactggatg gacgtcgcgc ggctcggcgt
//
LOCUS       DASTFC010000369        12407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_573320,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000369 DASTFC010000000
VERSION     DASTFC010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 12407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12407
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573320"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFO88_10830"
     CDS             complement(<1..974)
                     /locus_tag="VFO88_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9546058.1"
                     /translation="MSRFLERLESGPVVVGDGGTGALLAARVPRLRVPEEANIRAPEA
                     VIGVHTAFIRAGAELIETNTFGANRRKLSALLLEHRLDEIVERGVKLAREAREISGAE
                     VFVAGSIGPLGDLEGTHGAEDAYAVFLEQATLLEGRGVDLFMVETFFDLEELEAAVAA
                     VRSVSSLPIVAQLTFDEDAETLAGVAARDAAERLSSAGVAAVGANCGLGPGAALAALA
                     EMAPAANGLALAAQPNVGLPSRSGGRIVYPNATPDYFAEFAAQARSLGARIVGGCCGT
                     TPAQIAAIRAAVESEREPRVPLEVAEERDAAELAAIRPAFADERGTLL"
     gene            complement(1005..2327)
                     /locus_tag="VFO88_10835"
     CDS             complement(1005..2327)
                     /locus_tag="VFO88_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546059.1"
                     /translation="MIETITPAVCGSRRRRQIAIALFTVAAIGSAALVGVALGTLGLV
                     LGGTAALVAAAAIAGLAALRELGLVRVPLPQSRRQVPQWWHARLPLPVWSAGYGAGLG
                     AGFFTFQPVATFWVACAAAVALGRPLAAGAGFALYGVGRALMVVLPARRDPEVTAAVE
                     RLARLRPALVRVNGVVLAGCAVALALALTLAPPAGAGVMKLGPTGLNLDPTASKGVFA
                     HTSGSKGDAQVVVRAPGAAPITYPGSSPSLHGPLLAYEDGPDVRIVRWQTGDEVARMD
                     NAQKPQLRWPWLAFRRPMDGGRKYLRLKNMQTGQSRLVARAGPKKDLGRPALHRGRLA
                     WHTAGPTGSQIFLYNLATRRRQVVARTKIALLANPALGGARIAWTEQRRTGSKLFVKR
                     IGTKQRRQVARTRQGNRVFWTTALDNDFAYVTRWFVPEMTARINKIRL"
     gene            complement(2399..2998)
                     /locus_tag="VFO88_10840"
     CDS             complement(2399..2998)
                     /locus_tag="VFO88_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546060.1"
                     /translation="MRRPDAVLDEAGGALAANLASGLTRRSFLGRVGAAFLGLVGGSA
                     VAAAARPDDAEAFHFCGHIWTTGSCPTPYTLPRIDRRGYPIRPKDARPIDNLGRLVDA
                     NGFPIHENGQRMTGPDGNPLARGPRTRVCEDWVPERFGIDAVTQGSWYRCCGGQIRKL
                     VDCCSRSRRRINGDASLVGYCYTGRRVFCVMYYDTGLPC"
     gene            complement(2985..3860)
                     /locus_tag="VFO88_10845"
     CDS             complement(2985..3860)
                     /locus_tag="VFO88_10845"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HET9546061.1"
                     /translation="MEAVAQVATGVVLLWAAGSKLRVRRELPDVLGAYGVPPGLRAPL
                     AIALVAVEAALGVLLVVRVAPELAAAGALALAGTFVAAPLVARLRGIERIRCGCFGVK
                     ERATSVVILRGVAFAALAALAVFGGRIEASAPSRDTLVLVALGVLALAVVVLAVLVLA
                     LYRQVGVLSLRLGPRAALEVPEEGPPLETPAPALAGLDRRGAELVAFVSEACRICREL
                     TPSFRALAREGLAVRIVSEEDESSAFRRWNVPGSPFVVHVVDGVVAAKGLVNTLEQLD
                     GLVAAGTARRRNAAA"
     gene            complement(3932..5056)
                     /locus_tag="VFO88_10850"
     CDS             complement(3932..5056)
                     /locus_tag="VFO88_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET9546062.1"
                     /translation="MAGRVLAAIVLALAAAPAATAQTLPACGGATTTTCVVSVERNDV
                     TVPYPSGPDYEILAMEIGPPANEDFYVNVARTGGAWTLDLADVWEIVLNTGSVAPSET
                     FARARNVSVVRGGSATSRTVGFTFQPVRMADDSCNANGVCPPVATRLLPGYLDAWVND
                     LSYIDDPADRAAMRGFDLATNADWVSSPLQLDWETNAIVLDAANAHFEPGGTTVFVGS
                     AEFRIPNAMLQRLYGVDDPASLTAAAFRVSAGTGPAPAVAVTVGSGEVHVTMTNITFS
                     KRKLRIVGDMRPRKPRDVRAVRVSATTGVVGFRKALPRGSKVRGYRALCLSGGQARRG
                     QALRSPVRIRNLTPGRGYRCSVRATSRAGLGRAASFRLRP"
     gene            complement(5088..6449)
                     /locus_tag="VFO88_10855"
     CDS             complement(5088..6449)
                     /locus_tag="VFO88_10855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9546063.1"
                     /translation="MRVVAGVFFYPRGGSAQVVRSLARALPPAGWEATIAAGSLGQPG
                     ERGHAATFYAGLDVHAVDYTPAFALADPLAAEVPFQPSYEDRPGSPDRVFAAVPDDAY
                     ERLVDAWADALARAGAAEADVLHLHHLTPLHEAARRRFPDVPVVGELHGTELAFLREL
                     EAGAPHGWPHAEAWHERMRRWAHACERLVVSAADAVDEAERLLGIDPARVVTIPNGID
                     PERFDRRPLGATGRLAYWRRRLVEEPRGWDESGLPGSVRYADADLEPFRAGGPVLLYS
                     GRFTAVKRVPLLVRAHARASRRLARPAPLVLVGGHPGEWEGEHPLAAVRAAGSRDVFL
                     AGWHDHDELPAAMNAADLLVLPSVADAFGLVLVEAMACGLPVIACDAHGPRTIVEPGE
                     TGWLVPPDDEDALADAIVDAVDDGEERRRRGALAYERSRARYAWPALAERLARVYADA
                     AGA"
     gene            complement(6468..7100)
                     /locus_tag="VFO88_10860"
     CDS             complement(6468..7100)
                     /locus_tag="VFO88_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9546064.1"
                     /translation="MDERKRARVLDEPSRARVYELLSGAEDGLDARELAERSGLHANT
                     VRWHLAALAEARLVTSRPEATGGRGRPRVVYRAEGEAPGEPENYRLLAGLLASSLARS
                     PDGGAEAEAAGAAWGASVTSPAATADEAVAGVVRLLADEGFRPELVDGELRMRRCPFH
                     DLALAHGQVVCRLHLGLLRGSLEASGAGVEVSALQPWVRPDLCVARLDAA"
     gene            7240..8340
                     /locus_tag="VFO88_10865"
     CDS             7240..8340
                     /locus_tag="VFO88_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9546065.1"
                     /translation="MKYVATVLLGVAALFTTTMALGAADEANKARVAAEEGAATHTDH
                     VEDGTAGTAAATATQSFAGQTAENGEELAAAREPFPAALPEAPEGDVAEVNLVVVDKP
                     VEIAPGVSYNVWAWGYDKESATALGPAIHVREGQLVKFTITNEGSIPHSMDFHAARIA
                     PNEAFKDVMPGESFSFEFVAGDPGVYMYHCGTKPVLAHIANGMYGAIVVEPADLPPAD
                     EEYVLVSSEWYLDSPGIDEPANLSMDKAKAMTPDWVTWNGYAAQYVHHPLTADPGDTV
                     RFWVVAAGPSLDTDFHVVGTVFERAWVNAAMHDPQIGPQTVEVPAGGGGVFDVKIDDP
                     GTYPIVSHSFASVELGQVGILKVGEPKGGGSH"
     gene            8478..8975
                     /locus_tag="VFO88_10870"
     CDS             8478..8975
                     /locus_tag="VFO88_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546066.1"
                     /translation="MLAVAALAVALLAAAGCGGDDAADDVAEATTGLTDTGEAAETLE
                     DALDDAETTLPDGLTLTLEEQNDSGISGEVKLSPTSEGALEVEIELDGSDGGPHPAHI
                     HEGSCDDLNPEPAFPLEDVVDGESETTVEISVDDLVATEYAVNVHESAENVDVYVACA
                     DVRSF"
     gene            8989..11196
                     /locus_tag="VFO88_10875"
     CDS             8989..11196
                     /locus_tag="VFO88_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9546067.1"
                     /translation="MSPFSRAAAQAIVALRWPIVVAWVAAAVLVVVALPSLQEAGEET
                     SLLGLVPKDAEALETELRLAELFEAPVVTHTHVVQRDPEGLSEPAFERAVERADAVAG
                     GADPELPEIQLALPLLNARGVVPGSRENGTTIVTYVFYDPRATGIFAQEEVTKRFAET
                     RFAEPDDALVGVTGAAPARVEEWRTIERGLPWVTAATILLIALVLGIHFRSFVAPVVT
                     LLAAGIAYIVALRSVAWFGQGIGVTIPRDAEPILVVLLLGVVTDYAVFFLHGMQERLA
                     EGRPRLDAAREATGGYLSIVVTAGLIVAGGTASLMAGQLEFFRAFGPGMALTVLVSLA
                     VAITLVPALMAILGERTFWPRPRPRERRPRGPNRVAYFATSRPVALVLAVAAIAALAF
                     ACRGLVETNLGVTQIAGLSTETEPRRAQLAATDGFAQGILSPTVILLEELDLPGDLAA
                     LRELEESLAAEEGVAGALGPSTEAQEGLPELLVAADAGAARVLVVLDHEPVGGPAIDA
                     IERLDERLPGLLADAGLENARASFAGDTALAQETVETITHDLLRIGIAAFLVNFLLLA
                     LFLRALVAPLYLVLASGLALAASIGLATFLFQTVLGYGELTYYVPFAVAVLLLSLGSD
                     YNVFVVGRIWQEAERMPLRDAIATAAPRASRAIGVAGLALALSFATLALIELRQFREF
                     AFTMAVGVMLDAFLIRAVLIPALVSLVGEKSWWPRRRRAAPPEIEPEPETGAA"
     gene            complement(11198..12082)
                     /locus_tag="VFO88_10880"
     CDS             complement(11198..12082)
                     /locus_tag="VFO88_10880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9546068.1"
                     /translation="MIAVGVATCGRPDALRTCLDALAAQRLAPAEVVVVDQAPSDEAR
                     AAVAQAGLAGARYVEQERLGLSASRNLALELATADILAVTDDDCAPDRGWLEAVAAAF
                     ERAPRPDAVTGPILPLGEQPPGGFAVSLRTAAEPVDHHGRMQPWGVGSGANLAAPVEL
                     LRRHGGWDMRLGAGSRGRAAEDADLAYRLLVAGAVIRYEPAAVVRHAWQSRDRRLATR
                     WSYGYGVGAMCGLALGRRDPYAVRMLAGYGRPHLRPLARALVRRDGFAAAERARALAS
                     VVPGLAYGLRAGLKPPRA"
     gene            complement(12079..>12407)
                     /locus_tag="VFO88_10885"
     CDS             complement(12079..>12407)
                     /locus_tag="VFO88_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546069.1"
                     /translation="HTLDAYVPSGAALGPAGRLSRRLQRRVVPLPDLVLLLDASGRTL
                     HERSGEYDADVLERWREAYAALERRVPHLVKLDAERPPDAVRRDAEKHVWRRYGELRG
                     RRAGAR"
BASE COUNT         1624 a         4641 c         4701 g         1441 t
ORIGIN      
        1 tggaggagcg tgccgcgctc gtcggcgaac gcgggacgga tcgcggcgag ctccgcggcg
       61 tctcgctcct ccgcgacctc gagcgggacg cgcggctcgc gctcgctctc gacggcggcg
      121 cggatggcgg cgatctgtgc cggcgtcgtg ccgcagcagc cgcccacgat ccgcgcgccg
      181 agcgagcggg cctgggcggc gaactccgcg aagtagtccg gcgtcgcgtt cgggtagacg
      241 atgcggccgc cggagcggct cgggaggccg acattcggct gtgcggcgag cgcgagcccg
      301 ttcgcggccg gcgccatctc ggcgagcgcg gcgagcgcgg cgcccgggcc gagcccgcag
      361 ttcgcgccga ccgccgcgac cccggccgac gagagccgct ccgccgcgtc acgcgccgcg
      421 acccccgcga gcgtctccgc gtcctcgtcg aacgtgagct gcgcgacgat cggcagcgag
      481 gagaccgacc gcaccgcggc gaccgccgcc tcgagctcct cgaggtcgaa gaacgtctcg
      541 accatgaaga ggtcgacgcc gcggccctcg agaagcgtcg cctgctcgag gaagacggcg
      601 taggcgtcct ccgcgccgtg cgtgccctcc aggtcgccga gcggcccgat cgaccccgcg
      661 acgaagacct cggcaccgga gatctcccgc gcctcgcggg cgagcttcac gccgcgctcg
      721 acgatctcgt ccagccggtg ctcgaggagc agcgccgaga gcttgcgccg gttcgccccg
      781 aaggtattcg tctcgatgag ctccgccccc gcccggatga acgccgtgtg gacgccgatc
      841 accgcctccg gcgcgcggat gttcgcctcc tcgggaacgc gcaggcgcgg cacgcgggcg
      901 gcgaggagcg cgcccgtgcc cccgtcgccg acgacgaccg gccccgactc gaggcgctcg
      961 aggaagcggc tcacgcagcc agtgtaggtc gcgccggccg tccgtcagag ccggatcttg
     1021 ttgatccgcg ccgtcatctc cgggacgaac cagcgcgtca cgtacgcgaa gtcgttgtcg
     1081 agcgcggtcg tccagaagac gcggttcccc tgcctcgtcc gcgcgacctg acgccgctgc
     1141 ttcgtgccga tccgcttgac gaagagcttc gagccggtgc ggcgctgctc cgtccacgcg
     1201 atcctcgcgc cgccgagcgc cgggttcgcg aggagcgcga tcttcgtgcg ggcgacgacc
     1261 tgccggcggc gggtggcgag gttgtagagg aagatctgcg agcccgtcgg cccggccgtg
     1321 tgccaggcga gccgcccgcg gtggagcgcc gggcggccga ggtccttctt cgggccggcg
     1381 cgcgcgacga ggcgggactg gccggtctgc atgttcttga gccgcaggta cttgcggccg
     1441 ccgtccatcg gccggcggaa cgcgagccag ggccagcgga gctgcggctt ctgcgcgttg
     1501 tccatccgcg cgacctcgtc accggtctgc cagcggacga tccggacgtc tggaccgtcc
     1561 tcgtacgcga gaagcggccc gtggagcgag ggcgacgagc cgggatacgt gatgggcgcc
     1621 gcgccgggcg cccggacgac gacctgcgcg tcgcccttcg agccggaggt gtgcgcgaag
     1681 acccccttgc tcgcggtcgg gtcgaggttg agcccggtcg ggccgagctt catcacgccg
     1741 gcgcccgccg gcggcgcgag cgtgagggcg agcgcgagcg cgaccgcgca gccggcgagg
     1801 acgacgccgt tcacgcgcac gagcgccggg cggagacgcg cgagccgctc gaccgccgcc
     1861 gtcacctcgg ggtcgcgccg cgcgggcagc acgaccatga gcgcgcgccc gaccccgtag
     1921 agggcgaagc cggcccccgc ggcgagcgga cgcccgagcg cgacggcggc cgcacacgcg
     1981 acccagaagg tcgcgacggg ctggaaggtg aagaaccccg caccgagccc cgcgccgtac
     2041 cccgccgacc acaccggcag gggcaggcgc gcgtgccacc actgcggcac ctggcggcgc
     2101 gactgcggga gcgggacacg cacgaggccg agctcccgga gcgcggcgag cccggcgatc
     2161 gcggccgcgg cgacgagcgc cgccgtcccg ccgagcacga gcccgagcgt gccgagcgcg
     2221 acgcccacga gcgccgccga cccgatcgcg gccacggtga agagggcgat tgcgatctgt
     2281 ctccgtctgc ggctcccgca cacggcggga gtgatcgtct cgatcatgga gagccctcag
     2341 ggcgaccagg cgcccgagac ccccgcggcg agggcggcga cggcgacgac gccttcgatc
     2401 agcacggcag ccccgtgtcg tagtacatga cgcagaagac acggcggccc gtgtagcagt
     2461 agccgacgag cgaggcgtcg ccgttgatcc ggcgccggct gcgcgagcag cagtcgacga
     2521 gcttgcgaat ctgcccgccg cagcagcggt accaggagcc ctgcgtgacc gcgtcgatcc
     2581 cgaaccgctc cggcacccag tcctcgcaca cgcgcgtgcg cgggccgcgc gcgagcgggt
     2641 tgccgtccgg cccggtcatc cgctgtccgt tctcgtggat cgggaagccg ttcgcgtcga
     2701 cgagccgccc gaggttgtcg atcggccgcg cgtccttcgg gcggatcggg tagccgcgcc
     2761 ggtcgatccg cgggagcgtg tacggggtcg ggcacgagcc ggtcgtccag atgtggccgc
     2821 aaaagtggaa ggcctccgcg tcgtccggcc gcgcggcggc cgcgaccgcc gacccgccga
     2881 cgagcccgag gaacgcggcg ccgacgcgcc cgaggaacga gcggcgcgtg aggcccgagg
     2941 cgaggttcgc ggcgagcgcg ccgccggcct cgtcgagcac ggcgtcaggc cgccgcattc
     3001 cgcctccgcg cggtgccggc cgcgacgagc ccgtcgagct gctcgagcgt gttcacgagg
     3061 cccttcgccg cgacgacgcc gtccacgacg tggacgacga aggggctccc ggggacgttc
     3121 cagcgtcgga aggccgagct ctcgtcctcc tcggacacga tccggacggc gagcccctcg
     3181 cgcgcgagcg cccggaagga cggcgtgagc tcgcggcaga tccggcaggc ctccgagacg
     3241 aaggcgacga gctccgcccc gcggcggtcg aggcccgcga gcgccggcgc gggcgtctcg
     3301 agcggcggtc cctcctccgg cacctcgagc gcggcgcgcg ggccgaggcg caggctgagg
     3361 acgccgacct ggcgatagag cgcgaggacg agcacggcga gcacgacgac ggcgagcgcg
     3421 aggacgccga gcgccacgag gacgagcgtg tcgcgcgacg gcgcggacgc ctcgatccgc
     3481 ccgccgaaga cggcgagcgc ggcgagggcc gcgaaggcga ccccgcgcag gatgacgacg
     3541 ctcgtcgccc gctccttcac gccgaagcac ccgcagcgga tccgctcgat cccgcgcaga
     3601 cgcgcgacga gcggcgcggc gacgaacgtc ccggcgagcg cgagcgcgcc ggccgccgcg
     3661 agctcgggcg cgacgcggac gacgagcagg acgccgaggg ccgcctcgac cgcgacgagc
     3721 gcgatcgcga gcggcgcgcg caggccgggc gggacgccgt aggcgccgag gacgtcggga
     3781 agctcccgcc ggacgcgcag cttcgagccc gcggcccaga ggaggaccac gcccgtggct
     3841 acctgggcga cggcttccac ccggagcagg gtagcgacgt gtgcggcgga gcgtggaagg
     3901 acgcggtaaa ccccctccgc ggccccgccg cctacggacg gaggcggaag gaggcggcgc
     3961 ggccgaggcc ggccctgctc gtcgcgcgga cggagcagcg gtagccgcgg cccggggtga
     4021 ggttgcggat ccggaccggc gaccgcagcg cctgcccgcg ccgcgcctgg ccgccggaga
     4081 ggcagagcgc gcggtagccg cgcaccttcg acccgcgcgg cagcgccttg cggaacccga
     4141 cgacgccggt cgtcgcgctc acgcgcacgg cgcgcacgtc gcgcggcttg cgcggccgca
     4201 tgtcgccgac gatgcggagc ttccgcttcg agaaggtgat gttcgtcatc gtcacgtgca
     4261 cctcgcccga ccccacggtc accgcgacgg cgggagctgg gccggtgccc gcgctcacgc
     4321 ggaacgcggc ggcggtgagg ctcgccgggt cgtcgacccc gtagagccgc tggagcatcg
     4381 cgttcgggat ccggaactca gcgctcccga cgaagacggt cgtcccgccc ggctcgaagt
     4441 gcgcgttcgc cgcgtcgagg acgatcgcgt tcgtctccca gtcgagctgg agcggcgacg
     4501 agacccagtc cgcgttcgtc gccaggtcga agccgcgcat cgccgcccgg tcggccgggt
     4561 cgtcgatgta cgagaggtcg ttcacccacg cgtcgaggta gcccggcagg agccgcgtcg
     4621 cgaccggcgg acagacgccg ttcgcgttgc acgagtcgtc cgccatccgc acgggctgga
     4681 acgtgaagcc gaccgtccgg ctcgtcgccg accctccgcg tacgacgctc acgttgcgcg
     4741 cgcgggcgaa cgtctccgag ggcgcgaccg agccggtgtt cagcacgatc tcccagacgt
     4801 cggcgaggtc gagcgtccac gcgccgccgg tgcgggcgac gttcacgtag aagtcctcgt
     4861 tcgcgggcgg gccgatctcc atcgcgagga tctcgtagtc cggcccgctc gggtacggga
     4921 cggtgacgtc gttccgctcg acggagacga cgcaggtcgt cgtcgtggcg ccgccgcacg
     4981 cggggagcgt ctgcgccgtg gcggccggcg cggccgcaag ggcgaggacg atcgctgcga
     5041 gcactctccc ggccacgctc gaactatcgg aggccgagcg gacggaccta agccccggcc
     5101 gcgtccgcgt acacgcgcgc gaggcgctcg gcgagcgcgg gccaggcgta ccgggcccgg
     5161 ctgcgctcgt acgcgagcgc gccgcggcgg cggcgctcct ccccgtcgtc caccgcgtcg
     5221 acgatcgcgt ccgcgagcgc gtcctcgtcg tcgggcggga cgagccagcc ggtctcgccg
     5281 ggctcgacga tcgtccgcgg cccgtgcgcg tcgcacgcga tcaccggaag cccgcacgcc
     5341 atcgcctcga cgaggacgag cccgaacgcg tccgccacgg acggcaggac gagcaggtcg
     5401 gccgcgttca tcgccgcggg cagctcgtcg tggtcgtgcc agccggcgag gaacacgtcc
     5461 cgcgaccccg ccgcgcgcac ggcggcgagc gggtgctctc cctcccactc gcccgggtgg
     5521 ccgccgacga ggacgagcgg cgccgggcgc gcgagccgcc gcgacgcccg cgcgtgcgcg
     5581 cgcacgagga gcgggacgcg cttcaccgcc gtgaagcgcc cgctgtagag gaggaccggc
     5641 ccgccggcgc ggaacggctc gaggtcggcg tccgcgtagc gcaccgagcc cggcaggccg
     5701 ctctcgtccc acccgcgcgg ctcctcgacg agccgccggc gccagtacgc gagccgcccg
     5761 gttgcgccga gcgggcggcg gtcgaagcgc tccgggtcga tcccgttcgg gatcgtgacg
     5821 acgcgggcgg ggtcgatccc gagcaggcgc tccgcctcgt cgaccgcgtc cgcggccgag
     5881 acgacgagcc gctcgcaggc gtgggcccag cggcgcatcc gctcgtgcca ggcctcggcg
     5941 tgcggccacc cgtgcggcgc gccggcctcg agctcgcgga ggaaggcgag ctccgtcccg
     6001 tgcagctcgc cgacgaccgg cacgtcggga aagcggcggc gcgcggcctc gtggagcggc
     6061 gtcagatggt ggaggtggag gacgtccgcc tcggccgcgc ccgcccgtgc gagcgcgtcg
     6121 gcccaggcgt ccacgagccg ctcgtacgcg tcgtccggta ccgccgcgaa cacgcggtcg
     6181 gggcttccgg gccggtcctc gtaggacggc tggaacggca cctcagcggc gagcgggtcg
     6241 gcgagcgcga acgccggcgt gtagtcgacc gcgtgcacgt cgaggcccgc gtagaacgtc
     6301 gccgcgtgcc cgcgctcgcc cggctgcccg agcgagccgg cggcgatggt cgcctcccac
     6361 ccggcggggg gcagcgcgcg cgcgagcgag cggacgacct gggccgatcc gccgcgcgga
     6421 tagaagaaga cgccggcgac gacgcgcacc gggcgattct gacgtggcta cgcggcgtcg
     6481 aggcgggcga cgcagaggtc cgggcgcacc cacggttgga gcgccgacac ctcgacgccg
     6541 gccccgcttg cctcgagcga gccgcggagg agtcccagat ggagccggca gacgacctgc
     6601 ccgtgcgcga gcgcgaggtc gtggaacgga cagcggcgca tgcgcagctc gccgtccacg
     6661 agctccggcc ggaagccctc gtcggcgagc aggcggacga cgcccgcgac cgcctcgtcc
     6721 gccgtcgcgg cgggcgacgt gaccgacgcg ccccaggccg cgcccgccgc ctcggcctcc
     6781 gccccgccgt cgggcgagcg ggcgagcgag ctcgcgagga gcccggcgag gagccggtag
     6841 ttctccggct cgcccggcgc ctcgccctcc gcgcggtaca cgacccgcgg gcggccccga
     6901 ccgccggtcg cctccggccg cgacgtgacg agccgcgcct cggcgagcgc ggcgaggtgc
     6961 cagcggaccg tgttcgcgtg caggcccgag cgctcggcca gctcgcgcgc gtcgagcccg
     7021 tcctccgcgc cggagaggag ctcgtagacg cgcgcccggc tcggctcgtc gagcacgcgc
     7081 gcccgcttcc gttcgtccat cacgtcacga gcgtgccacg gaggctcccg cgccgcgtcc
     7141 gtaggaaccc cgtaccgcga ttccgcggcg gcgcggatgc cgtgcggccg gattcctccg
     7201 atgctcctcg tagaaaacga gaccagtgag gtgagcgaga tgaagtacgt cgcaacggtg
     7261 ctcctggggg tcgcagccct cttcaccacg acaatggcgc tcggcgccgc ggacgaggcg
     7321 aacaaggccc gcgtcgcagc cgaggagggc gccgccacgc acacggacca cgtcgaggac
     7381 ggcaccgccg gaaccgccgc cgcgacggcg acgcagagct tcgccgggca gaccgcggag
     7441 aacggcgagg agctcgccgc ggcgcgcgag cccttccccg cggcgctccc cgaggcgccc
     7501 gagggtgacg tcgccgaggt gaacctcgtc gtcgtcgaca agccggtcga gatcgcgccc
     7561 ggcgtgagct acaacgtctg ggcctggggc tacgacaagg agagcgcgac ggcgctcggc
     7621 ccggccatcc acgtgcgcga gggccagctc gtcaagttca cgatcacgaa cgagggctcg
     7681 atcccgcact cgatggactt ccacgcggcg cggatcgccc cgaacgaggc cttcaaggac
     7741 gtcatgccgg gcgagagctt ctcgttcgag ttcgtcgccg gcgacccggg cgtctacatg
     7801 taccactgcg gcacgaagcc ggttctcgcg cacatcgcga acgggatgta cggcgcgatc
     7861 gtcgtcgagc cggccgacct gccccccgcc gacgaggagt acgtgctcgt ctcgagcgag
     7921 tggtacctcg actcccccgg catcgacgag cccgccaacc tctcgatgga caaggcgaag
     7981 gccatgacgc cggactgggt gacctggaac ggctatgcgg cccagtacgt gcaccacccg
     8041 ctgaccgccg acccgggcga caccgtgcgc ttctgggtcg tcgcggccgg gccgagcctc
     8101 gacacggact tccacgtcgt cggcacggtc ttcgagcggg cctgggtgaa cgccgcgatg
     8161 cacgacccgc agatcggccc gcagacggtg gaggtgcccg cgggcggcgg cggcgtcttc
     8221 gacgtcaaga tcgacgaccc gggcacgtat ccgatcgtga gccactcgtt cgcctcggtc
     8281 gagctcggcc aggtcgggat cctcaaggtc ggcgagccga agggcggggg cagccactga
     8341 gaccacgact cgcgcacctc acgcgaagcg gatcggcgcc ctcgggggcg ccgatccgcg
     8401 tttcggggat ctgcgatccg gcgaggcttc aagacgttct gaaggcgatg aagggaggcg
     8461 ctgtgcggag aacgacgatg ctggcggtcg cggcgctcgc cgtggcgctc ctcgcagcgg
     8521 cgggctgcgg cggcgacgat gcggcagacg acgtggccga ggcgaccacc gggctcaccg
     8581 acacgggcga ggccgccgag acgctcgagg acgcgctgga cgacgccgag acgacgctcc
     8641 ccgacgggct gacgctcacc ctcgaggagc agaacgactc cggcatcagc ggcgaggtca
     8701 agctctcgcc gacgtccgag ggcgcgctcg aggtcgagat cgagctcgac ggaagcgacg
     8761 gcgggccgca tcccgcgcac atccacgagg ggagttgcga cgacctgaac ccggagcccg
     8821 cgttcccgct cgaggacgtg gtggacggcg agtcggagac gaccgtcgag atctccgtgg
     8881 acgacctcgt cgcgaccgag tacgcggtga acgtccacga gtcggccgag aacgtcgacg
     8941 tgtacgtcgc ctgcgcggac gtccggagct tctgagccgg ccgcacacgt gagccccttc
     9001 tcccgcgctg ccgcccaggc gatcgtcgct cttcgctggc cgatcgtcgt cgcgtgggtg
     9061 gcggcggccg tgctcgtggt cgtcgcgctg ccgagcctcc aggaggcggg cgaggagacg
     9121 tcactcctcg ggctcgtccc gaaggacgcg gaggcgctcg agacggagct ccgcctcgcc
     9181 gagctcttcg aggcgccggt cgtcacgcac acccacgtgg tgcagcgcga cccggagggg
     9241 ctctccgagc cggcgttcga gcgggcggtc gagcgggccg acgcggtcgc gggcggggcc
     9301 gacccggagc ttcccgagat ccagctcgcg cttccgctcc tgaacgcgcg cggcgtcgtc
     9361 ccgggctcgc gcgagaacgg gacgacgatc gtcacgtacg tcttctacga cccgagggcg
     9421 accgggatct tcgcgcagga ggaggtgacg aagcgcttcg cggagacgcg cttcgcggag
     9481 ccggacgacg cgctcgtcgg ggtgaccggc gcggccccgg cgcgggtcga ggagtggcgg
     9541 acgatcgagc gcggcctccc gtgggtcacg gccgcgacga tcctcctgat cgcgctcgtc
     9601 ctcgggatcc acttccgctc gttcgtcgcg cccgtcgtga cgctcctggc ggcggggatc
     9661 gcctacatcg tcgccctgcg gagcgtcgcg tggttcgggc aggggatcgg cgtgacgatc
     9721 ccgcgggacg cggagccgat cctcgtcgtc ctcctgctcg gcgtcgtcac cgactacgcg
     9781 gtcttcttcc tgcacgggat gcaggagcgg ctggccgagg gacgcccgcg cctcgacgcg
     9841 gcgcgcgagg cgaccggcgg ctacctctcg atcgtcgtca cggccggcct gatcgtcgcc
     9901 ggcgggacgg cgtcgctcat ggcgggacag ctcgagttct tccgcgcgtt cgggccgggg
     9961 atggcgctca ccgtgctcgt ctcgctcgcc gtcgcgatca cgctcgtgcc ggcgctcatg
    10021 gcgatcctcg gcgagcggac gttctggccg cggccccgcc cgcgggagcg gcggccgcgc
    10081 gggccgaacc gcgtcgcgta cttcgccacc tcccgtccgg tcgcgctcgt gctcgcggtc
    10141 gcggcgatcg cggcgctcgc cttcgcctgc cgcggcctcg tcgagacgaa cctcggcgtg
    10201 acccagatcg ccggcctctc gaccgagacg gagccgcgcc gcgcccagct cgcggcgacg
    10261 gacgggttcg cgcagggcat cctctcgccg accgtgatcc tcctcgagga gctcgacctg
    10321 ccgggcgacc tcgcggcgct ccgggagctc gaggagtcgc tcgcggccga ggaaggcgtc
    10381 gccggcgccc tcggcccgtc gacggaggcg caggaggggc tccccgagct gctcgtcgcc
    10441 gccgacgccg gagccgcgcg ggttctggtc gtcctcgacc acgagccggt cggcggcccg
    10501 gcgatcgacg cgatcgagcg gctcgacgag cggctgcccg ggctcctcgc ggacgcgggg
    10561 ctcgagaacg cccgggcgag cttcgcgggc gacaccgcgc tcgcgcagga gaccgtcgag
    10621 acgatcacgc acgacctcct gcggatcggg atcgcggcgt tcctcgtgaa cttcctcctg
    10681 ctcgcgctct tcctgcgcgc gctcgtggcg ccgctctacc tcgtgctcgc gtccgggctc
    10741 gcgctcgcgg cgtcgatcgg gctcgcgacg ttcctcttcc agacggtgct cggctacggc
    10801 gagctcacgt actacgtgcc gttcgcggtc gcggtgctcc tgctctcgct cgggtcggac
    10861 tacaacgtgt tcgtcgtcgg gcggatctgg caggaggcgg agcggatgcc gctccgggac
    10921 gcgatcgcga ccgcggcgcc gcgcgcgtca cgggcgatcg gggtcgccgg gctcgcgctc
    10981 gcgctctcct tcgccacgct cgcgctcatc gagctgcgcc agttccgcga gttcgccttc
    11041 acgatggcgg tcggcgtgat gctcgacgca ttcctcatcc gcgcggtgct gatcccggcg
    11101 ctcgtctcgc tcgtcggcga gaagagctgg tggccgcgcc ggcggcgcgc cgctcccccg
    11161 gagatcgagc cggagccgga aaccggcgcc gcctgaccta ggcgcgcgga ggcttcaacc
    11221 ccgcgcgcag cccgtacgcg agcccgggga cgacgctcgc gagcgcgcgg gcgcgctcgg
    11281 cggccgcgaa cccgtcccgc cggacgagcg cgcgcgcgag cggccgcagg tgcgggcggc
    11341 cgtacccggc gagcatccgg acggcgtacg gatcgcgccg gccgagggcg agcccgcaca
    11401 tcgcgccgac cccgtagccg tacgaccagc gggtcgcgag gcggcggtcg cgggactgcc
    11461 acgcatggcg gacgaccgcg gccggctcgt agcggatcac ggcgccggcg acgaggagcc
    11521 ggtacgcgag gtcggcgtcc tccgccgccc gcccgcgcga gccggcgccg agccgcatgt
    11581 cccagccgcc gtgtcggcgc aggagctcga cgggcgctgc gaggttcgcc ccgctcccca
    11641 cgccccacgg ctgcatccgg ccgtggtggt cgaccggctc ggcggccgtc cggagcgaga
    11701 ccgcgaagcc gcccggcggc tgctcgccga gcgggaggat cggccctgtc accgcgtccg
    11761 ggcgcggcgc gcgctcgaac gcggcggcga cggcctcgag ccagccgcga tcgggcgcgc
    11821 agtcgtcgtc ggtgacggcg aggatgtccg cggtcgcgag ctcgagcgcg aggttgcgcg
    11881 aggccgagag cccgagccgc tcctgctcga cgtagcgcgc gcccgcgagc ccggcctgcg
    11941 cgaccgcggc gcgggcctcg tccgagggcg cctggtcgac gaccaccacc tccgccggtg
    12001 cgagccgctg ggcggcgagg gcgtcgaggc acgtccggag cgcgtcgggc cggccgcacg
    12061 tcgcgacccc gaccgcgatc accgcgcccc cgcccgccgc ccgcggagct cgccgtagcg
    12121 gcgccagacg tgtttctccg cgtcgcgccg aacggcgtcg ggcggccgct ccgcgtcgag
    12181 cttcacgagg tgcgggacgc gccgctcgag cgccgcgtac gcttcccgcc agcgctcgag
    12241 gacgtccgcg tcgtactcgc cgctccgctc gtgcagcgtc cgcccggacg cgtcgaggag
    12301 gaggacgagg tcggggagcg ggacgacccg gcgctggagc cgccgggaga gccgcccggc
    12361 cggcccgagc gccgcgccgg aggggacgta cgcgtcgagc gtgtgcc
//
LOCUS       DASTFC010000370         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_721967,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000370 DASTFC010000000
VERSION     DASTFC010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721967"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFO88_10890"
     CDS             complement(<1..366)
                     /locus_tag="VFO88_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546070.1"
                     /translation="MRLEEAQTLFEPEGVYLNTASYGLPPRPAWDELQRALDEWRHGR
                     TSWEPWSEATDRARRAFARLVGVREDDVAEGATVSQLLGLVAGSLPEGTRVLAPEHDF
                     TSLLFPFLAQAGRGIEVRTA"
     gene            complement(373..1293)
                     /gene="lipA"
                     /locus_tag="VFO88_10895"
     CDS             complement(373..1293)
                     /gene="lipA"
                     /locus_tag="VFO88_10895"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9546071.1"
                     /translation="METSLPVAERPRRPEWMKVRAPSADGRYFEVRKLIHGQNLHTIC
                     EEARCPNIAECWGRGTATFQILGETCTRACRYCYVHSGRPSDPPDPLEPFRLAHAAAQ
                     MGLKHVVVTSVDRDDVPDRGAGHYAATIRALKRKLPEASVEVLTPDFLGVEEDALRTV
                     LAARPEVFNHNIETVRRLHRRMRGAKADYDKALWLLARAKELAGYPVLTKSGIIVGLG
                     ETNDEVVETMRDLRAHGVDVVTIGQYLQPSAKHAAIDRWVHPDEFRWFREQGEAMGFG
                     SVFSGPLVRSSYRADEQRHAAETGRGAVAY"
     gene            complement(1323..1958)
                     /locus_tag="VFO88_10900"
     CDS             complement(1323..1958)
                     /locus_tag="VFO88_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546072.1"
                     /translation="MRNGIGTTVRGIIGIAAVACALALPGAASAGLLTGTLSTSEASY
                     CDPDVNQYFKRWGDSALYRLVPGGSFEGTHGWRLANGAKVVSGNEPWYVKGYGSRSLY
                     LPAGSTATSPTTCFDFGDWHARFFVRNRGGGGSLEVDILVRSLVGVLSVIDGGTVRAD
                     GTWDPSPRVSALVTNVGGLLGTEAVSFRLKAKGSGAAFQVDDVFLDPFRSR"
     gene            2123..>3208
                     /locus_tag="VFO88_10905"
     CDS             2123..>3208
                     /locus_tag="VFO88_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9546073.1"
                     /translation="MSTVAGVGERTPAHLDLEVQQSIEEGRARRLTELARDELVSLVV
                     ANLLLLATAVSTALLIPSARTPSLLAAALLVGAYALAYRLEFELSTGSAVPTQLVLVP
                     MLFVLPLGLVPLTVAGAIALACAVDVARGLMHPERVLLQLGSNAWHAVGPVLVLGLAG
                     EAAPSLAHWPLYVAALAAQFAFDFVAPAVREWLVLRVRPRMHLAQMGWVYLIDACLAP
                     IGLAVAFVAIESPAAVVLSLPLIGLLLVFSRERRVRIDSELELRDAYRGTVFLLGDVV
                     EADDEYTGAHSRDVVDLTLAVANALGLPAQARRDAEFVALLHDVGKVRIPNEIINKPG
                     KLTPEERAIIETHTIEGERMLHRVGGLL"
BASE COUNT          424 a         1192 c         1128 g          464 t
ORIGIN      
        1 cgccgtccgc acctcgatcc cgcgccccgc ctgggcgagg aagggaaaga ggagcgacgt
       61 gaagtcgtgc tcgggcgcga ggacgcgcgt cccctcaggg agcgagccgg cgacgaggcc
      121 gaggagctgc gacacggtcg ccccctcggc gacgtcgtcc tcgcgcacgc cgacgagccg
      181 cgcgaacgcc cgccgcgccc ggtcggtcgc ctcgctccac ggctcccagc tcgtccggcc
      241 gtgacgccac tcgtcgagcg cgcgctggag ctcgtcccag gccggccgcg gcgggagccc
      301 gtagctcgcc gtgttgagat agacgccctc cggctcgaag agcgtctggg cctcctccag
      361 gcgcacgccg gcctagtacg cgacggcgcc gcggccggtc tcggccgcgt ggcgctgctc
      421 gtccgcgcgg tacgaggagc ggacgagcgg gccggagaag accgacccga agcccatcgc
      481 ctcgccctgc tcgcggaacc agcggaactc gtcgggatgc acccagcggt cgatcgccgc
      541 gtgcttcgcc gacggctgga ggtactggcc gatcgtcacg acgtcgacgc cgtgcgcacg
      601 caggtcgcgc atcgtctcga ccacctcgtc gttcgtctcg ccgaggccga cgatgatccc
      661 ggacttcgtg agcaccggat agccggcgag ctcctttgcc cgcgcgagga gccagagcgc
      721 cttgtcgtag tcggccttgg ccccgcgcat gcggcggtgg aggcggcgca cggtctcgat
      781 gttgtggttg aagacctccg gccgcgcggc gaggacggtc cggagcgcgt cctcctcgac
      841 gccgaggaag tccggcgtca gcacctcgac cgacgcctcg ggcagcttcc gcttgagcgc
      901 gcggatcgtc gcggcgtagt ggccggcgcc gcggtcgggg acgtcgtcgc ggtcgaccga
      961 ggtcacgacg acgtgcttca gccccatctg cgccgcggcg tgggccagcc ggaagggctc
     1021 gagcggatcc ggaggatccg acgggcgccc ggaatggacg tagcagtacc ggcacgccct
     1081 cgtgcacgtc tcgccgagga tctggaacgt cgccgtcccg cggccccagc actcggcgat
     1141 gttcgggcag cgagcctcct cacagatcgt gtggaggttc tgcccgtgga tgagcttcct
     1201 gacctcgaag taacgcccgt cggcgctcgg cgcgcgaacc ttcatccact ccgggcggcg
     1261 cggccgctcg gcgacgggaa gcgaggtctc cacgccgcga agtctacggg cttgcgtgcg
     1321 cgctaccggc tgcggaaggg atcgaggaag acgtcgtcga cctggaacgc cgcgccgctg
     1381 cccttcgcct tcagccggaa gctcactgcc tccgtcccga ggagcccgcc gacgttcgtg
     1441 acgagggcgc tcacccgcgg agacgggtcc cacgtgccgt cagcgcgcac ggtcccgccg
     1501 tcgatcacgc tgaggacgcc gacgaggctg cggacgagga tgtcgacctc gagcgagcct
     1561 ccgccgcccc ggttgcgcac gaagaagcgc gcgtgccagt cgccgaagtc gaagcacgtc
     1621 gtcggcgagg tcgcggtcga gccggccggg aggtagaggg agcgggagcc gtagcccttc
     1681 acgtaccacg gctcgttgcc gctcacgacc ttcgcgccgt tcgcgagccg ccagccgtgc
     1741 gtgccctcga acgacccgcc ggggacgagg cggtacaggg ccgaatcgcc ccagcgcttg
     1801 aagtactggt tcacgtcggg gtcgcagtac gacgcctcgc tcgtcgagag ggtcccggtc
     1861 aggaggcccg cgctcgccgc gcccggaagc gcgagcgcgc acgcgaccgc cgcgatgccg
     1921 atgatgcctc gaaccgtcgt cccgatcccg tttctcacga ttcttcctcc cgccggaagc
     1981 cgccgcagtg gtgagctgaa ccgcattgtc cgagcgccct cgaggcgcgc caatcacccg
     2041 aaaaggggac ctgccgccct cttgatccct cgaacgaggc acggaatcaa gtaccggccc
     2101 gcacgagccg atttccagct tcgtgtccac cgttgcgggg gtcggcgagc gaacgcctgc
     2161 gcacctcgac ctcgaggtgc agcagtcgat cgaggaggga cgggcgcggc gcctcacgga
     2221 gctcgcgcgc gacgagctgg tctcgctcgt cgtcgcgaac ctgcttctcc tggcgaccgc
     2281 cgtctcgacc gcccttctca tcccgtcggc gcggacgccc tcgctcctcg cggcggcgct
     2341 cctcgtcggg gcgtacgcgc tcgcctaccg gctcgagttc gagctgagca cggggagcgc
     2401 ggtcccgacg cagctcgtcc tcgtcccgat gctcttcgtc ctgccgctcg ggctcgtccc
     2461 gctcaccgtg gccggcgcga tcgcgctcgc gtgcgccgtg gacgtcgcgc gcgggctcat
     2521 gcacccggag cgcgtcctcc tccagctcgg ctcgaacgcc tggcatgcgg tcgggcccgt
     2581 gctcgtcctc gggctcgcgg gcgaggccgc cccgtcgctc gcgcactggc cgctctacgt
     2641 cgcggcgctc gcggcccagt tcgcgttcga cttcgtcgcg ccggcggtgc gggaatggct
     2701 cgtcctccgc gtccggccgc ggatgcacct cgcccagatg ggctgggtgt acctcatcga
     2761 cgcgtgcctc gccccgatcg ggctcgcggt cgcgttcgtg gcgatcgaga gccccgccgc
     2821 cgtcgtgctc tcgctcccgc tgatcgggct cctgctcgtg ttctcgcgcg agcggcgcgt
     2881 gcggatcgac tcggagctcg agctgcgcga cgcctaccgc ggcacggtct tcctgctcgg
     2941 ggacgtggtc gaggccgacg acgagtacac cggcgcgcac agccgcgacg tcgtcgacct
     3001 cacgctcgcc gtggcgaacg cgctcggcct cccggcgcag gcgcggcgcg atgcggagtt
     3061 cgtcgcgctc ctccacgacg tcggcaaggt gcggatcccg aacgagatca tcaacaagcc
     3121 ggggaagctg accccggagg agcgcgcgat catcgagacg cacacgatcg agggcgagcg
     3181 gatgctccat cgcgtcggcg gcctcctc
//
LOCUS       DASTFC010000371         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_722207,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000371 DASTFC010000000
VERSION     DASTFC010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722207"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFO88_10910"
     CDS             complement(<1..291)
                     /locus_tag="VFO88_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546074.1"
                     /translation="MRTLRLAAAAVAGATFVFTGLAVVRAGSVLDWIPHWLTPFLALA
                     FVAPATVGLLVALRRPRNRIAWILLLGPFAMTVQLPLGLALGEGWALQLDRAM"
     gene            455..1123
                     /locus_tag="VFO88_10915"
     CDS             455..1123
                     /locus_tag="VFO88_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546075.1"
                     /translation="METMRLQPLSGVVFVALVALATVGLAGETPGSNASASELVSFYG
                     DSWRQFFAAIVLAASIPFLVVFAAGIAGAAATSAPGREERSVWRFVVIAGAAVTAAVV
                     LVQALLHMALTDGADQGVPESALQAVNTVAGSMWVPFFAALGLMMLGAAGCLIPGAGA
                     HRWLGWTALVLGVALFIPAADFFAALLSAVWLVVASVTMARGAGRAPERRPSMARSTA
                     ATAD"
     gene            1567..>2849
                     /locus_tag="VFO88_10920"
     CDS             1567..>2849
                     /locus_tag="VFO88_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9546076.1"
                     /translation="MTAAESSRRAALVARWGAETLVFRAASALVLLHALDDAFLNRQP
                     GVPWDQHWLAGLISVVVAAAALVAFPSLRPGLRAAVSFVFGLLATVNGALHVIHITKE
                     GPSGSDVTGVLALAAGIVLIALALWIPWRHRGEGVRTGRRRWLNRGIALVAGALVLYA
                     FVFPTSLAMIQTHKYREPIGSPPSAEYQTVSFESSDGLELSGWYRASRNGAAVVVVHG
                     GGGDRSGAVAHAELLARHGFGVLVYDSRGRGESEGSPVGFGWGWPKDVAGALGFLRER
                     SDVDPERIGGLGLSRGADTLIRVAAADRGLKAVIADGATGGSFADYRNLGEEAEGAPF
                     YLAMYTAARVFSGESPGEPLKDAVARISPTPLLLIATGRSLPAERDFNRIYAEAAREP
                     FELWELPDVDHIAAIRQRPDEYERRVVEFFDDALL"
BASE COUNT          383 a         1043 c         1023 g          400 t
ORIGIN      
        1 catcgcccgg tccagctgca gcgcccagcc ttcgccgagc gcgagcccga gcgggagctg
       61 gaccgtcatc gcaaacggcc cgagcagcag gatccaggcg atccgattgc ggggccggcg
      121 aagcgcgacg agcagcccga ccgtcgccgg cgccacgaag gcgagcgcaa ggaacggcgt
      181 cagccagtgc ggaatccagt ccagcacgct gccggcccgg acgaccgcga gacccgtgaa
      241 gacgaatgtc gctcccgcga cggcagccgc ggcgaggcgg agcgtgcgca tgccgagatc
      301 ttccgccgat ccgggccgaa ccgcaatacg tgccagcacc gaagcgaggt ccggtggcgg
      361 cgccgagacg agactcgtgc ggccgccgat gcgccgcgtc cggctcgggc ctacggtcgg
      421 gcgaaagccg agtcacccac ccgaaaggag tgtcatggag accatgcggc tacagccgct
      481 cagcggagtc gtcttcgtcg cgcttgtcgc gctggcgacc gtcggcctcg cgggcgagac
      541 gcccggatcg aacgcctccg cgagcgagct cgtctcgttc tacggggatt cgtggcgcca
      601 gttcttcgcc gcgatcgtgc tcgcggcgtc gatcccgttc ctcgtcgtgt tcgccgccgg
      661 catcgccggc gccgccgcca cctccgcgcc cgggcgagaa gagcggtccg tctggcggtt
      721 cgtcgtgatc gccggcgcgg cggtcaccgc cgccgtcgtg ctcgttcagg ccctcctcca
      781 catggcgctc accgacggcg ccgatcaggg cgtgccggag agcgcgctcc aggccgtgaa
      841 caccgtcgcg gggagcatgt gggtcccctt cttcgcggcg ctcggactca tgatgctcgg
      901 cgccgcgggt tgcctgattc ccggtgcggg cgcccacagg tggctgggct ggacggcgct
      961 cgtgctcggc gtcgcgctct tcatccccgc cgccgacttc ttcgcggcgc tcctctccgc
     1021 cgtctggctc gtcgtcgcga gcgtgacgat ggcgcgcggc gccggccggg cgccggagcg
     1081 ccggccgtcc atggcccgat ccaccgcggc cacggccgac tgaggggaag gagacgaggc
     1141 cgcgatcagg gcaggaacga cggcgacgac gcaatggggg cggggagcgg cgtcgctctc
     1201 cgccccttcg ccagcgcctc gcgcaaagcc gtgccccgac cgaaagtgag cgcaggcgcc
     1261 tcgacccgtg aacggcgagt gcggcgggcg catcagtccg cgggtcgatc tcgctcagac
     1321 aaacggttga cacgcatccg acgccggtgg gaagcgctcc cgcgccggct cggaccgacc
     1381 accgaggaga aatcaacctc ggtgccgacg acggcggggc cggagcttcg tagcgtcgtc
     1441 ggcgacggcg cgaggcaacg aagggagcga gatggaagcg ggcgcgatcg gaccaccgac
     1501 gaacggctcg gtcacgtccg ccccgcccgc gttgcgcgcc ggtgtccttc gggcacccgg
     1561 gtacgaatga cggccgcgga atcctcccgc cgcgccgcgc tcgtcgcgcg ctggggcgcc
     1621 gagacgctcg tgttccgcgc tgcgagcgcg ctcgtcctcc tgcacgctct cgacgacgcc
     1681 ttcctgaacc gtcagcccgg cgtcccgtgg gaccagcact ggctcgcggg cctcatcagc
     1741 gtcgtcgtcg ccgcggcggc cctggtcgca ttcccgtccc tgcgcccggg cctacgggcg
     1801 gccgtctcgt tcgtcttcgg cctccttgcg accgtcaacg gcgcgctgca cgtcatccac
     1861 atcaccaagg aggggccgtc cgggagcgac gtgaccggcg tgctcgcgct cgcggccggc
     1921 atcgtcctca tcgcgctcgc cctctggatc ccctggcgac accgcggcga gggcgtgcgg
     1981 acgggccgtc gccgctggct caaccgcggt atcgcgctcg tcgccggcgc gctcgtgctc
     2041 tatgccttcg tcttcccgac cagcctggcg atgatccaaa cgcacaagta ccgcgagccg
     2101 atcggctccc cgccgagcgc ggagtaccag acggtgtcct tcgagtcctc cgacgggctc
     2161 gagctctccg ggtggtatcg cgcgtcccgc aacggtgcgg ccgtcgtcgt cgtccacgga
     2221 ggaggaggcg accggagcgg ggcggtcgcg cacgcggagc tcctcgcccg tcacggcttc
     2281 ggcgtcctcg tgtacgactc ccggggccgc ggcgagagcg aaggcagccc ggtcgggttc
     2341 ggctggggtt ggccgaagga cgtcgccggc gcgctcgggt tcctgcgcga gcgttcggac
     2401 gtcgaccccg agcgcatcgg cgggctcggc ctctcgagag gcgccgacac cctgatccgg
     2461 gtcgcggccg cggacagagg gctgaaggcc gtcatcgccg acggcgcgac cggtggatcc
     2521 tttgccgact accggaacct cggcgaggag gcggaagggg cgccgttcta cctggcgatg
     2581 tacacggccg cgcgggtctt ctccggagag tcgcccggcg agccgctgaa ggacgctgtg
     2641 gcgaggatct ctccgacccc gctgctgctg atcgcgacgg gccgcagcct cccggccgag
     2701 cgcgacttca atcggatcta cgcggaagcg gcgcgcgagc cattcgagct ctgggagctc
     2761 cccgatgtcg atcacatcgc ggcgattcgc cagcggcctg acgagtacga gcggcgggtg
     2821 gtcgagttct tcgacgacgc cctcctcga
//
LOCUS       DASTFC010000372         9922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1364700,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000372 DASTFC010000000
VERSION     DASTFC010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364700"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VFO88_10925"
     CDS             complement(<1..1118)
                     /locus_tag="VFO88_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9546077.1"
                     /translation="MSATDTLINTLFDGRYRILRKLGTGGMANVYLAEDEDLGRRVAI
                     KILNDRYASDEAFTERFRREAKSAAALSHPNIVSIYDRGDADGRPYIAMEVIEGKSLK
                     ELIVGSGPLPISEAVAYAKQILSATRFAHRHGIIHRDIKPHNILLGHEDRLKVTDFGI
                     ARSGASQMTEVGSIMGTAQYLSPEQARGAPVTAASDLYSVGIVLYEMLTGKTPFNGET
                     PIEIAMKHVNDPPRPPSEHRPDIPPELDQVVLRALSKDIHERYGSAEEFSADLDRVEA
                     GLPVSPETTAAATAVLTGADATAATQVLRDEAGTRVMPRGTAAPARRPPTYPPDYGYE
                     PPRKRRRWVPWLLVLLFAAAAIAAGWYVYSRVQDELSES"
     gene            complement(1139..2629)
                     /locus_tag="VFO88_10930"
     CDS             complement(1139..2629)
                     /locus_tag="VFO88_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9546078.1"
                     /translation="MNTQIRNLFLVFAFLFVALVGMSSYWLWRAPDLEARQGNPNLVV
                     RQLLIERGLIYAADGETVFARNREQEVDGQTWFRRRYPERGLAAHVHGYSTIQRSRTG
                     LEESLNDYLTGSNADLATLLDRAGDTLRGITRQGNDVVTTIDANAQRVALDALGGRCG
                     SVVALEPASGRVLVMASSPTFDPNLVENRFRQATRSNAPCDPAAPLFNRATAGFPSPA
                     FIPGSTFKVLTAAAALDSGQFSATSEFDDPGYCIEYGERVYNYSDQAGPRAFGRVDLG
                     DAVENSINSVFCNIGKELGPRVILDYAKEFGFYEDPPLETPGDERAPSGLWSNGRLFD
                     PEDPNQIDPGRLAFGQERLLVTPLQMAMVVATIANGGVLMRPHVVDRIVAPDGDVVTR
                     TRPDDLRRVVSADTAATLTPMMVRVVSSGTGTSAQIPGVEVAGKTGTAETGRQGQNDT
                     GFIAFAPAGSPRVAIAVYLQNQSGTGGATAAPIAKAVMEAILRSNP"
     gene            complement(2626..3921)
                     /locus_tag="VFO88_10935"
     CDS             complement(2626..3921)
                     /locus_tag="VFO88_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET9546079.1"
                     /translation="MSLRTRELVNLVVLGIVTTIGFVSVYLASQEVPEFSTTSLSYAA
                     FFLALFVVAHLIVRIRLPLADPHLLPIAGILTAIGLTMIYRIEPDTAFKQGVWVVIGV
                     ALFAGACLLLRDYRSLDALKYTCGIGAIVLLVLPAVPGLGLEVNGARLWVSVGPLQFQ
                     PGEFAKVLLVIFLAGYLRDNRELLSMGYGRIGVPSPKHFGPLLLIWGGAMLVLVQTND
                     LGGGLLLFSIFLAMLYVATGRWPYVAVGLALFAAGSWALYEAVPRVGDRIAIWLDPWA
                     EPDAAGYQLVQSIYSIAAGGIFGTGFGKGVLVRQDGETYIPYLETDFIYSAIAQELGL
                     AGAAAVILLYLVFCYRGFRIAMSADDGFSKLLAAGLTATLAIQTFIIIGGVSGLIPLT
                     GITLPFVSYGGSSIVANFLLLALLLMVSHRVNEGVEGRR"
     gene            complement(3965..5170)
                     /locus_tag="VFO88_10940"
     CDS             complement(3965..5170)
                     /locus_tag="VFO88_10940"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9546080.1"
                     /translation="MQVGDRAGLTDTGRRRRRNEDSYVLDPPLFAVADGMGGAQAGEV
                     ASRLAAAAFREYHEADELEPERRVEALVQEANRRVYERSREDANASGMGTTVTAALVE
                     GDSVAIAHVGDSRAYRIRGDALEQLTEDHSLVADLMRSGRLTPEEAEAHPQRSVITRA
                     LGTDPEVDVDTFAVGLEPDDVFLLCSDGLTTMVPDEEILRIVHESSSLDRAASALVKA
                     ANKRGGEDNVTVVLFRVDGDGADPPLDETVRLDAPDRDGDTDELEDTLTEADPVPPVA
                     EPTAAPAPAAPAARPAPAPPRRRRSRRAFAVVAALLFVALLLLVAFWGLANAHFVGAE
                     EDGSVAVYQGLPYDVSEGFPLYRPRYVSTLHAAQLTQEERAELFDHELMDYGAARDRL
                     ARYEDEGVP"
     gene            complement(5173..5637)
                     /locus_tag="VFO88_10945"
     CDS             complement(5173..5637)
                     /locus_tag="VFO88_10945"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9546081.1"
                     /translation="MEPVLLALKAAFLVVLYVFIWRVVRSAARDLRVAPQESFVLAPE
                     QARAITGEPPPAPARLVVVDGPSLERGTVFEPGPVALTFGRAGDNTAVLDTDDYASTH
                     HARIEASRDGVWLVDLGSTNGTWVNGEQVDGRHRLHDGDLVRIGQTELRYER"
     gene            complement(5650..6441)
                     /locus_tag="VFO88_10950"
     CDS             complement(5650..6441)
                     /locus_tag="VFO88_10950"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HET9546082.1"
                     /translation="MGVLRGIEHRLESLVEGAFGRAFRSHVQPVELARKLVKEMDDHK
                     TVSVSRVYVPNEYVLYLAPQDRESLPPERELLTELADYLAEHARREGYALVSTPRVLL
                     EEDEDLSVGEFGIATRMVHAKEASADAPATPAAPAVEPDAELGQTKIFKPAPDTAAVS
                     AAEAAELGLAHSPAVLVVRGKRHELGDGPTVIGRSRECTITIDDPNISRRHAEIRRQD
                     GAYWIADLGSTNGLEVNGRREQRARLSHGDRIVIGRTDLAFELPG"
     gene            complement(6471..8123)
                     /locus_tag="VFO88_10955"
     CDS             complement(6471..8123)
                     /locus_tag="VFO88_10955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9546083.1"
                     /translation="MANTPLVARAVSDRSPPAADGGSSSLILGRYRPLRPLGSGGSGS
                     VWLARDERGGREVALKVVRREGKAGSRAEREVEAATRLRHPNCLRALALDRDDGHVYV
                     TYEYVEGRTLRDALVAGGLDDAAVVEAAAQILEGLAHAHGKKIVHRDVKPANVMLAEG
                     DEVSVRLLDFGLARVEEADTLTAAGDVPGTLAYVSPERLGGTPGTGAADVWAVGVVLW
                     EGLTGRHPFAGASPVETARRIREGARPLARARPDLPTELCMLVDRMLDTDPRRRPAAR
                     RLPNALRDAFAGESRRPRPATSLATLKERALHSGLAAAFATGARLLLPFFPRGWPFAL
                     GLVTAGVALRSPRTGLALALAVPVLPLGNVALGLALAYSALAAGWLALFFRAPTSGLL
                     FLAGPALAPLGGIGLLPLVALRALTRVHQAALAAAGVLAAGAFGALAGGPVPVADRFP
                     DSLGLAATARPDTAAGAALGALGGHPGLLVLALVVAAATLAAPYALEHGAWGVAGWGS
                     GFLTAAMLGPTLAGGEPNAVLLVPGIWAAALWLGRDLLPHPR"
     gene            8201..9343
                     /locus_tag="VFO88_10960"
     CDS             8201..9343
                     /locus_tag="VFO88_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HET9546084.1"
                     /translation="MAVFERDRTDIDEPIEFDFFDESPTVEAAREEPKPRVRRRMPRR
                     PPGGGSNTPLIRLGLLIAGGIVLAVGLILWVNACQGARTGPYQSYMTEVEGVAGDSAQ
                     IGRDLNALIFSSGIQLEDLQSQLEGLQSAQAQVVRRTEALDAPGPLREQQESLVEVML
                     LRQSGLSGLAAAFSGITDAEAAQQAGSDLAVQSQRLVASDVVYEDFFRVRAQEIMEQQ
                     GVTGVAVPESTFVSNIELASAESWVLVVERLLRPPAEGGLRGNSIAGVRVLPEGTQLS
                     ASEDNTVIASQDLALEVSVENSGESQETQVVVTLTIQQSPEPLRSEQTIQSINPGDTR
                     TVTFRDFGLPQFGARTNVKVTVEPVPGETNTNNNTADYVVFFTIPD"
     gene            9351..9830
                     /locus_tag="VFO88_10965"
     CDS             9351..9830
                     /locus_tag="VFO88_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HET9546085.1"
                     /translation="MTDTAVAVLALVAAGVAVLALGLAVAFFLQLRKVRKAQVVLLGS
                     GRDDLVDFAVSLQARIDDVHRAVDEVGLALSRVDKRIDGCLSHTSIVRYDAYEDAGGH
                     QSASLAFLDAARSGVVVSAIQGRDYARIYVKELDRGRATVALSPEEQEAVDRAMATG"
BASE COUNT         1375 a         3503 c         3735 g         1309 t
ORIGIN      
        1 gactcgctga gctcgtcctg gacgcgcgag tagacgtacc agcccgcggc gatcgcggcc
       61 gcggcgaaga ggagcacgag cagccagggc acccagcggc gccgcttgcg cggcggctcg
      121 tagccgtagt cgggcgggta cgtcggcggg cgccgcgcgg gcgccgccgt gccgcggggc
      181 atcacgcgcg tgcccgcctc gtcccggagc acctgcgtcg cggcggtcgc gtccgctccg
      241 gtcaggacgg cggtcgccgc tgcggtcgtc tccggcgaga cgggaagccc cgcctcgacc
      301 cggtcgaggt cggcgctgaa ctcctccgcc gagccgtacc gctcgtggat gtccttcgag
      361 agcgcgcgca gcacgacctg gtcgagctcc ggcgggatgt cgggccggtg ctcggacggc
      421 gggcgcggcg ggtcgttcac gtgcttcatc gcgatctcga tcggcgtctc gccgttgaag
      481 ggcgtcttgc cggtgagcat ctcgtagagc acgatgccga ccgagtagag gtcggacgcg
      541 gcggtgaccg gggcgccgcg cgcctgctcc ggggagaggt actgcgcggt ccccatgatc
      601 gagccgacct ccgtcatctg cgaggcgccg gagcgggcga tcccgaagtc cgtcaccttc
      661 agccggtcct cgtggccgag gaggatgttg tgcggcttga tgtcccggtg gatgatcccg
      721 tgccggtggg cgaagcgggt cgcggagagg atctgcttcg cgtacgcgac cgcctccgag
      781 atcgggagcg gccccgagcc gacgatgagc tccttcaggc tcttcccctc gatcacctcc
      841 atcgcgatgt acgggcgccc gtccgcgtcg ccgcggtcgt agatcgagac gatgttcggg
      901 tgcgagagcg ccgccgccga cttcgcctcg cgccggaagc gctccgtgaa cgcctcgtcc
      961 gacgcgtagc ggtcgttcag gatcttgatc gcgacgcgcc gcccgagatc ctcgtcctcc
     1021 gcgaggtaga cgttcgccat cccgcccgtc ccgagcttgc gcaggatgcg gtagcggccg
     1081 tcgaagagag tgttgatcaa ggtgtcggtg gctgacatcg ccgaattctc cgaaaagcct
     1141 agggattgct ccgcaggatc gcctccataa cggccttggc gatcggcgcg gcggtcgcgc
     1201 cgccggtgcc ggactggttc tgcaggtaga ccgcgatcgc gacgcgcggc gagcccgcgg
     1261 gcgcgaacgc gatgaagccg gtgtcgttct gtccctggcg cccggtctcg gcggtgcccg
     1321 tcttccccgc cacctcgacg ccggggatct gcgcggacgt ccccgtcccg gacgagacca
     1381 cgcgcaccat catgggcgtc agggtggctg ccgtgtcggc gctcacgacg cgccggaggt
     1441 cgtcgggacg cgtccgggtg acgacgtcgc cgtcgggcgc gacgatccgg tcgacgacgt
     1501 gcgggcgcat gagcacgccg ccgttcgcga tcgtcgccac gaccatcgcc atctggagcg
     1561 gcgtcacgag cagccgctcc tgtccgaacg cgagccggcc cgggtcgatc tggttcgggt
     1621 cctccgggtc gaacagccgt ccgttcgacc agaggccgct gggcgcgcgc tcgtcgcccg
     1681 gcgtctcgag cggcgggtcc tcgtagaagc cgaactcctt cgcgtagtcg aggatgacgc
     1741 gcggcccgag ctccttcccg atgttgcaga agacggagtt gatcgagttc tcgaccgcgt
     1801 cgccgaggtc gacccggccg aacgcgcgcg ggcccgcctg gtcggagtag ttgtagaccc
     1861 gctcgccgta ttcgatgcag tagccggggt cgtcgaactc gctcgtcgcg ctgaactggc
     1921 ccgagtcgag cgcggcggcc gccgtcagca ccttgaacgt cgagccgggg atgaacgccg
     1981 gcgacgggaa gccggccgtc gcccggttga agagcggcgc cgccgggtcg cacggcgcgt
     2041 tcgagcgggt cgcctgccgg aagcggttct cgaccaggtt cgggtcgaac gtcggcgacg
     2101 aggccatgac gagcacgcgt ccggaggcgg gctcgagcgc gacgacggag ccgcagcggc
     2161 cgccgagcgc gtcgagcgcg acgcgctgcg cgttcgcgtc gatcgtcgtc acgacgtcgt
     2221 tcccctggcg cgtgatcccg cgcagcgtgt cgccggcccg gtcgaggagc gtggcgaggt
     2281 cggcgttcga gcccgtgagg tagtcgttca gcgactcctc gaggcccgtc cgggagcgct
     2341 ggatcgtcga gtagccgtgg acgtgcgccg cgaggccgcg ctccgggtac cggcggcgga
     2401 accacgtctg gccgtccacc tcctgctcgc ggttccgcgc gaagaccgtc tcgccgtccg
     2461 ccgcgtagat gagcccgcgc tcgatcagga gctggcggac gacgaggttc gggttcccct
     2521 gccgcgcctc gaggtccggc gcgcgccaga gccagtacga cgacatcccg acgagcgcga
     2581 cgaagaggaa ggcgaagacg aggaagaggt tgcggatctg cgtgttcacc ggcggccctc
     2641 cacgccctcg ttcacgcggt gcgagaccat gaggagcagc gcgagcagga ggaagttcgc
     2701 cacgatgctc gacccgccgt acgagacgaa cgggagcgtg atcccggtca gcgggatgag
     2761 cccgctgacg ccgccgatga tgatgaacgt ctggatcgcg agcgtcgccg tgaggcccgc
     2821 ggcgaggagc ttcgagaacc cgtcgtccgc gctcatcgcg atccggaagc cgcggtagca
     2881 gaagacgagg tagaggagga tcaccgcagc cgcgcccgcg agcccgagct cctgcgcgat
     2941 cgccgagtag atgaagtcgg tttcgaggta cgggatgtac gtctcgccgt cctggcgcac
     3001 gaggacgccc tttccgaacc cggtgccgaa gatcccgcct gccgcgatcg agtagatcga
     3061 ctggacgagc tggtagccgg cggcgtccgg ctcggcccac ggatcgagcc agatcgcgat
     3121 ccggtcgccc acgcgcggca cggcctcgta gagcgcccac gagcccgcgg cgaagagggc
     3181 gaggccgacc gcaacgtacg gccaccggcc ggtcgccacg tagagcatcg cgaggaagat
     3241 cgagaagagg aggagcccgc cgccgaggtc gttcgtctgg acgagcacga gcatcgcgcc
     3301 gccccagatg aggagcagcg ggccgaagtg cttcggcgac ggcacgccga tccggccgta
     3361 gcccatcgag aggagctccc ggttgtcgcg caggtagccc gccaggaaga tgacgagcag
     3421 caccttcgcg aactccccgg gctggaactg gagcggcccg acgctcaccc agaggcgcgc
     3481 gccgttcacc tcgagcccga gccccgggac cgcgggaagg acgagcagga cgatcgcgcc
     3541 gatcccgcac gtgtacttga gcgcgtcgag cgagcggtag tcgcggagga gcaggcaggc
     3601 gccggcgaag agggcgacgc cgatcacgac ccagacgccc tgcttgaacg ccgtgtccgg
     3661 ctcgatccgg tagatcatcg tgagcccgat cgcggtgagg atcccggcga tcgggaggag
     3721 atgcgggtcg gcgagcggca gccggatccg cacgatcagg tgcgcgacga cgaagagcgc
     3781 gaggaagaag gcggcgtacg agagcgacgt cgtcgagaac tccggcacct cctggctcgc
     3841 gaggtagacg gagacgaacc cgatcgtcgt cacgatcccg aggacgacga ggttcacgag
     3901 ctcgcgcgtg cggaggctca cgagtgctcc ttccggaagc ggtcgactgc tgccgggagg
     3961 ggcactatgg aacgccctca tcctcgtagc gggcgaggcg gtcgcgcgcg gcgccgtagt
     4021 ccatgagctc gtggtcgaag agctcggcgc gctcctcctg cgtgagctgc gcggcgtgga
     4081 gcgtgctcac gtagcgcggg cggtagagcg ggaagccctc gctcacgtcg tacgggaggc
     4141 cctggtacac ggcgacgctg ccgtcctcct ccgcgccgac gaagtgcgcg ttcgcgaggc
     4201 cccagaacgc gacgagcagg agcagcgcga cgaagaggag cgcggcgacg accgcgaacg
     4261 cgcgccggct cctccggcgc cgcggcgggg cgggcgccgg gcgcgcggcc ggcgccgcgg
     4321 gcgcaggtgc ggcggtcggc tcggccaccg gcggcaccgg atcggcctcg gtgagcgtgt
     4381 cctcgagctc gtcggtgtcc ccgtcgcggt cgggcgcgtc gagccgcacc gtctcgtcga
     4441 gcggcgggtc cgcgccgtca ccgtccacgc ggaagaggac gacggtcacg ttgtcctcgc
     4501 cgccccgctt gttcgcggcc ttgacgagcg cgctcgccgc ccggtcgagc gaggagctct
     4561 cgtggacgat ccggaggatc tcctcgtccg ggaccatcgt cgtcagcccg tccgagcaga
     4621 ggaggaagac gtcgtccggc tcgaggccga ccgcgaacgt gtcgacatcc acctcggggt
     4681 cggtgccgag cgcgcgcgtg atcacggagc gctgcggatg cgcctcggcc tcctccggcg
     4741 tcaggcgccc gcttcgcatg aggtcggcga cgagggagtg gtcctccgtg agctgctcga
     4801 gcgcgtcgcc gcggatccgg tacgcgcgcg agtcgcccac gtgcgcgatc gcgacgctgt
     4861 cgccctcgac gagcgcggcc gtgaccgtcg tgcccatgcc gctcgcgttc gcgtcctcgc
     4921 ggctgcgctc gtagacgcgg cggttcgcct cctggacgag tgcctcgacg cggcgctcgg
     4981 gctcgagctc gtccgcctcg tggtactcgc ggaaggcggc cgcggcgagc cgcgacgcga
     5041 cctcgccggc ctgggcgccg cccatcccgt ccgcgaccgc gaagagcggc ggatcgagga
     5101 cgtagctgtc ctcgttgcgc cgcctccggc gcccggtgtc cgtgagcccc gcgcggtctc
     5161 cgacctgcat cgtcatcgct cgtacctgag ctccgtctgg ccgatccgga cgaggtcgcc
     5221 gtcgtggagc cggtgccggc cgtcgacctg ctcgccgttc acccaggtcc cgttcgtcga
     5281 gccgaggtcg acgagccaca cgccgtcccg ggacgcctcg atgcgcgcgt gatgggtcga
     5341 ggcgtagtcg tccgtgtcga ggacggccgt gttgtccccc gcccggccga aggtcagcgc
     5401 gaccggcccc ggctcgaaga ccgtcccgcg ctcgagcgac gggccgtcca cgacgacgag
     5461 ccgcgcgggg gccggcggcg gctcgccggt gatcgcgcgc gcctgctccg gggcgaggac
     5521 gaagctctcc tgcggcgcga cgcgcaggtc gcgagccgcc gagcgcacga cgcgccagat
     5581 gaacacgtag aggacgacga ggaaggcggc cttgagtgcg agaaggacgg gctccaccgt
     5641 gtcgcgggcc tagcccggca gctcgaaggc gagatcggtg cggccgatca cgatccggtc
     5701 gccgtgcgag aggcgcgcgc gctgctcgcg gcgcccgttg acctcgagcc cgttcgtcga
     5761 gccgaggtcg gcgatccagt acgcgccgtc ctggcggcgg atctccgcat ggcggcgcga
     5821 gatgttcggg tcgtcgatcg tgatcgtgca ctcgcgcgag cggccgatca cggtcgggcc
     5881 gtcgccgagc tcgtgccgct tcccgcggac gacgaggacg gccggcgagt gcgcgagccc
     5941 gagctcggcg gcctcggccg ccgagaccgc cgccgtgtcg ggcgccggct tgaagatctt
     6001 cgtctggccg agctccgcgt ccggctcgac ggcaggcgcc gcgggtgtcg caggtgcgtc
     6061 ggccgaggcc tccttcgcgt gcaccatccg cgtggcgatg ccgaactccc cgacggagag
     6121 atcctcgtcc tcctcgagca gcacgcgtgg cgtcgagacg agcgcgtagc cctcccggcg
     6181 cgcgtgctcg gcgagatagt cggcgagctc ggtcaggagc tcgcgctccg gcgggaggct
     6241 ctcccggtcc tgcggcgcga ggtagaggac gtactcgttc gggacgtaca cgcgcgagac
     6301 ggagaccgtc ttgtggtcgt ccatctcctt cacgagcttc cgcgcgagct cgaccggctg
     6361 gacgtgcgag cggaacgcgc gcccgaacgc gccttcgacg agggactcca ggcgatgctc
     6421 gatgccgcgg aggacgccca ccgccgcatt ctgcccgagg cggggacttc ctagcgcggg
     6481 tgggggagca ggtcccgccc gagccagagc gcggcggccc agattcccgg tacgagcagc
     6541 acggcgttcg gctcgccgcc cgcgagcgtg gggccgagca tcgccgcggt caggaacccc
     6601 gagccccatc cggcgacgcc ccacgcgccg tgctcgaggg cgtacggggc ggcgagcgtc
     6661 gccgccgcga ccacgagcgc gagcacgagc agcccggggt ggccgccgag cgcgccgagc
     6721 gccgcgcctg ccgcggtgtc cggccgcgct gtggccgcga ggccgagcga gtccgggaag
     6781 cgatccgcga cgggcacggg ccctcccgcg agcgcgccga acgccccggc cgcgaggacg
     6841 cccgccgcgg cgagcgccgc ctggtgcacg cgggtgagcg cgcgcagcgc gacgagcggg
     6901 agcagcccga tcccgccgag cggcgcgagc gccggccccg cgaggaagag gagcccgctc
     6961 gtcggcgcgc ggaagaagag cgcgagccag ccggccgcca gcgccgagta cgcgagcgcg
     7021 aggccgagcg cgacgtttcc gagcgggagc accggaaccg cgagggcgag cgcgagcccc
     7081 gtgcgcggcg agcggagcgc gacgcccgcc gtgacgagcc cgagggcgaa cggccagccg
     7141 cgcgggaaga acggcaggag cagccgcgcc ccggtcgcga acgcggccgc gagccctgag
     7201 tggagcgcgc gctccttgag cgtcgcgagc gaggtcgcgg ggcgcggccg gcgactctcc
     7261 ccggcgaacg cgtcgcggag cgcgttcggc aggcggcggg cagccgggcg gcggcgcggg
     7321 tcggtgtcga gcatccggtc gacgagcatg cagagctcgg tcgggaggtc ggggcgcgcg
     7381 cgggcgagcg gccgggcgcc ctcgcggatc cgccgggcgg tctcgaccgg cgacgcgccc
     7441 gcgaaggggt gccggccggt gagcccttcc cagaggacga cgccgaccgc ccacacgtcg
     7501 gccgcgccgg tcccgggcgt gccgccgagc cgctccggcg agacgtaggc gagcgtcccc
     7561 gggacgtcgc cggccgccgt gagcgtgtcg gcctcctcca cgcgcgcgag cccgaagtcg
     7621 agcagccgga cgctcacctc gtcgccctct gcgagcatga cgttcgccgg tttcacgtcg
     7681 cggtggacga tcttcttccc gtgcgcgtgg gcgaggccct cgaggatctg ggcggcggcc
     7741 tccacgacgg ccgcgtcgtc gagcccgccg gcgacgagcg cgtcgcgcag cgtgcggccc
     7801 tcgacgtact cgtacgtgac gtagacgtgc ccgtcgtcgc ggtcgagcgc gagcgcgcgc
     7861 aggcagttcg gatggcgcag gcgggtggcc gcctcgacct cgcgctcggc gcgcgagccg
     7921 gccttgccct cgcggcggac gaccttcagc gccacctcgc gcccgccgcg ctcgtcccgc
     7981 gcgagccaga ccgagccgga gccgccgctc ccgagcgggc gcaggggccg gtagcggccg
     8041 aggatgagtg aggaggagcc cccgtccgcg gcgggcgggg agcggtcgct gacggcgcgg
     8101 gccacgaggg gcgtgttggc caccgtgcgc caaagtcctg cagttggggg gttcgtgggg
     8161 tcaccgtggt acgctcaccc aggtttttcg gcgattctgc gtggctgtgt tcgaacgcga
     8221 ccgaaccgat atcgacgaac cgatcgagtt cgacttcttc gacgagtcgc ccaccgtgga
     8281 ggcggcccgt gaggagccga agccgcgcgt gcggcggcgc atgccgaggc ggccgccggg
     8341 cggcgggtcg aacacgccgc tcatccgcct cggactgctg atcgcgggcg gcatcgtcct
     8401 cgccgtcggg ctcatcctct gggtgaacgc gtgccagggc gcccgcaccg gcccgtacca
     8461 gagctacatg accgaggtgg agggcgtcgc gggcgactcc gcgcagatcg ggcgcgacct
     8521 gaacgcgctc atcttctcct ccgggatcca gctcgaggac ctccagagcc agctcgaggg
     8581 cctgcagtcg gcccaggccc aggtcgtccg gcgcacggag gctctcgacg cgccgggacc
     8641 gctccgcgag cagcaggaga gcctcgtcga ggtcatgctc ctgcgccaga gcggcctgag
     8701 cgggctcgcc gccgcgttct ccgggatcac ggacgccgag gccgcccagc aggcgggcag
     8761 cgacctcgcg gtccagtcgc agcggctcgt ggcgagcgac gtcgtctacg aggacttctt
     8821 ccgcgtccgg gcgcaggaga tcatggagca gcagggcgtg accggcgtgg ccgtgccgga
     8881 gtcgacgttc gtgtcgaaca tcgagctcgc gagcgcggag tcgtgggtcc tcgtcgtcga
     8941 gcgcctgctg cgcccgccgg ccgagggcgg actgcgcggg aactcgatcg cgggcgtccg
     9001 cgtgctcccg gaggggacgc agctctcggc gagcgaggac aacaccgtga tcgcctcgca
     9061 ggacctcgcg ctcgaggtga gcgtcgagaa ctccggcgag agccaggaga cgcaggtcgt
     9121 cgtgacgctc acgatccagc agagccccga gccgctccgc tcggagcaga cgatccagtc
     9181 gatcaacccg ggtgatacgc ggacggtcac gttccgcgac ttcgggctcc cgcagttcgg
     9241 cgcgcggacg aacgtcaagg tgaccgtcga gcccgtgccg ggcgagacga acacgaacaa
     9301 caacacggcg gactacgtcg tcttcttcac catccccgac tgacgcttcc gtgaccgaca
     9361 ccgctgtcgc cgtcctcgcg ctcgtcgcgg ccggggttgc cgtgctcgcc ctcggcctcg
     9421 cggtcgcgtt cttccttcag ctccggaagg tgcggaaggc gcaggtcgtc ctcctcggca
     9481 gcggccggga cgacctcgtc gacttcgccg tctcgctcca ggcgcggatc gacgacgtgc
     9541 accgcgccgt ggacgaggtc gggctcgcgc tctcgcgcgt cgacaagcgg atcgacggct
     9601 gcctctcgca cacctcgatc gtccgctacg acgcctacga ggacgcgggc ggtcaccagt
     9661 ctgcctcgct cgcgttcctc gatgcggcgc gctcgggggt cgtcgtgagc gcgattcagg
     9721 gccgcgacta cgcgaggatc tacgtgaagg agctcgaccg cggacgcgcg accgtcgcgc
     9781 tctccccgga agagcaggag gcggtcgacc gtgcgatggc gaccgggtag ggccagagcg
     9841 tgacgtaccg gaaggcggag atccgcccga cgcgacccga ggacgaggcg ctcctcttcg
     9901 ggctcgcccg cctcgaccgc gg
//
LOCUS       DASTFC010000373         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1249686,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000373 DASTFC010000000
VERSION     DASTFC010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249686"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /gene="rodA"
                     /locus_tag="VFO88_10970"
     CDS             <1..1044
                     /gene="rodA"
                     /locus_tag="VFO88_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET9546086.1"
                     /translation="LASVSQNDVPGDPDFFLVRQSVNVAIGVAVLALATALNPELYRR
                     LRVPLYAAALILLTIVFVADQVRGTRRWIDIGFFQFQPSEIGKALLILVLAGFLADRA
                     RRLDEWGTTMGAVGLAAVPAVLVFLEPDLGTALVYVAILGAALFVAGTRWLHLATLAT
                     VGVLTVVSLFWLFPAVGIQVLEEYQIERLTGFLDPDHDPDGATYNVTQSITAVGSGGL
                     DGRGVEGATQTNMNYLPEHSTDFIFSSLAEQRGFFGAALLLLLYGLVVWRAIRTIALA
                     PSLFTAIVAGAIVVAFVFQVFVNVGMTIGVAPITGIPLPFVSYGGSSMLVTLGLIGLL
                     QAIHVRGRLALER"
     gene            complement(1068..1205)
                     /locus_tag="VFO88_10975"
     CDS             complement(1068..1205)
                     /locus_tag="VFO88_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546087.1"
                     /translation="MKKPSKQQIERARRKAENDPSGRRLRELVERGRAELEARRKTEG
                     R"
     gene            complement(1189..1320)
                     /locus_tag="VFO88_10980"
     CDS             complement(1189..1320)
                     /locus_tag="VFO88_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546088.1"
                     /translation="MAHEQVIDRDEVLTIMWALADLRTDVTYLRGIFEDWEDDEEAE"
     gene            1443..1643
                     /locus_tag="VFO88_10985"
     CDS             1443..1643
                     /locus_tag="VFO88_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546089.1"
                     /translation="MPENTPADREALAIEEADAWFEYLEAVRAHTRSRRYDEVEPWAW
                     ARLTQRLRLVEVERDRLRPAAA"
     gene            1844..>2367
                     /locus_tag="VFO88_10990"
     CDS             1844..>2367
                     /locus_tag="VFO88_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546090.1"
                     /translation="MLKWFRKKARSADDSPPEGGPAEQPAADDSPAAAEQDADAAAKP
                     RRRRGSRGGRGRKKTGAGAQAAAATESKPERKPREPRSGRGGRDGKDAKEDAKPKKRR
                     ASTRRTSAGGSRAALAGVKKEIVVSVDVGETRVAIVEDGKVSEVYLERPGRRSIAGNV
                     YKGVVDNVLPGMEAS"
BASE COUNT          317 a          862 c          838 g          350 t
ORIGIN      
        1 ctcgcctcgg tctcgcagaa cgacgtgccg ggcgacccgg acttcttcct cgtgcgccag
       61 tcggtgaacg tcgcgatcgg cgtggccgtg ctcgcgctcg cgacggcgct caacccggag
      121 ctctaccgcc gcctgcgcgt cccgctctac gcggcggcgc tcatcctgct cacgatcgtg
      181 ttcgtcgccg accaggtgcg cggcacgcgg cgctggatcg acatcggctt cttccagttc
      241 cagccctccg agatcgggaa ggcgctcctc atcctcgtcc tcgccggctt cctcgccgac
      301 cgggcgcgcc ggctcgacga gtgggggacg accatgggcg ccgtcgggct cgcagcggtc
      361 cccgccgtgc tcgtcttcct cgagccggac ctcgggacgg cgctcgtcta cgtcgcgatc
      421 ctcggcgcgg ccctcttcgt cgccggcacc cgctggctgc acctcgcgac gctcgcgacg
      481 gtgggcgtcc tgaccgtcgt ctcgctcttc tggctcttcc ccgccgtcgg gatccaggtg
      541 ctcgaggagt accagatcga gcgcctcacg ggcttcctcg atcccgacca cgacccggac
      601 ggcgcgacgt acaacgtgac gcagtcgatc acggccgtcg gctccggcgg cttagacggg
      661 cgcggggtcg agggcgcgac gcagacgaac atgaactacc tgccggagca ctcgaccgac
      721 ttcatcttct cctcgctcgc ggagcagcgc gggttcttcg gcgcagcgct cctcctgctc
      781 ctgtacgggc tcgtcgtgtg gcgtgcgatc cggacgatcg cgctcgcgcc gagcctcttc
      841 accgcgatcg tcgccggggc gatcgtcgtc gccttcgtct tccaggtctt cgtgaacgtc
      901 ggcatgacga tcggggtcgc gccgatcacc gggatcccgc tcccgttcgt gagctacggc
      961 gggagctcga tgctcgtgac gctcgggctg atcggcctcc tgcaggcgat ccacgtgcgc
     1021 gggcgcctgg cgctcgagcg ctagccgcgc ggcgtctcgc cgcgcctcta gcgaccctcg
     1081 gtcttccggc gcgcctcgag ctcggcccgc ccgcgctcga cgagctcgcg caggcgccgg
     1141 ccggacggat cgttctcggc cttccggcga gcccgctcga tctgctgctt actcggcttc
     1201 ttcatcgtct tcccaatcct cgaagatccc gcgcaggtac gtgacgtccg ttctcaggtc
     1261 ggcgagcgcc cacatgatcg tcaggacctc gtcgcgatcg atgacctgtt cgtgcgccac
     1321 ggattcatgg tggcgggaag gctcagtgac cgcaagacgc cttttcggtc ttgtccgaca
     1381 ggcgttcacg ggatgtccct tcccatgccg ggtccgtccg gtccgggtcc tagcctgcgc
     1441 ccatgcccga gaacacgcca gcagaccgag aagcgctcgc gatcgaggag gccgacgcct
     1501 ggttcgagta cctcgaggcc gtgcgtgcgc acacgcgctc gcgccggtac gacgaggtcg
     1561 agccgtgggc gtgggcccgg ctgacgcaac ggctgcgcct cgtcgaggtc gagcgcgacc
     1621 ggctcaggcc ggcggccgcg tagcgacggg ccgccgggcg tgaacggggg ccaggggggc
     1681 ccgctgctac gctgtgtcga aaggacgcag cggcccgccg gcgacggcga gcggcattcc
     1741 gcgcgtccaa tccgactgat gaagaagttt gaggccgaca cgctcgcgac ccgcgccctg
     1801 atcgggagcg cggggacgcg cgtgcgcccg aaaggagaca agcgtgctga aatggtttcg
     1861 gaagaaggcg cgctcggcgg acgactcgcc ccccgagggg ggccccgccg agcagcccgc
     1921 ggcagacgac tcgccggcgg ccgctgagca ggacgcggac gcggcggcga agccgcgccg
     1981 gcgccgcggg agccgcggcg gccggggccg caagaagacc ggcgcaggcg cccaggcggc
     2041 ggccgcgacg gagtcaaagc cggagcgaaa gccgcgcgag ccgcgcagcg gccgcggcgg
     2101 cagggacggg aaggacgcga aggaggacgc gaagccgaag aagcggcgcg cctccacccg
     2161 gcggacctcc gcgggcggct cgcgggccgc gctcgccggc gtcaagaagg agatcgtcgt
     2221 ctcggtcgac gtcggcgaga cccgcgtcgc gatcgtcgag gacggcaagg tctcggaggt
     2281 ctacctcgag cgcccgggcc gccggtcgat cgccggcaac gtctacaagg gcgtcgtcga
     2341 caacgtcctc cccggcatgg aggcgtc
//
LOCUS       DASTFC010000374         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1612324,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000374 DASTFC010000000
VERSION     DASTFC010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612324"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFO88_10995"
     CDS             <1..668
                     /locus_tag="VFO88_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9546091.1"
                     /translation="PGRDPRKLAPTTLARKLAAVRSFLRASLGPAHVPDLSLGGKRRR
                     RLPHAPRPDETNALLDGFEPATPLGLRNRALFELVYSAGLRAQEACDLELADVDFEAE
                     AVHVRGKGGKERVVPLGEEAAYRLGLYLERGRPALARGAVNALFLSTRGRPLETSTLR
                     RLLPNPHRLRHAFATDLMEGGADLRTIQELMGHSSLSTTQIYSHVDARRLRRIYDRAH
                     PRA"
     gene            643..1554
                     /locus_tag="VFO88_11000"
     CDS             643..1554
                     /locus_tag="VFO88_11000"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET9546092.1"
                     /translation="MTERTPVPDPAPDPQVEGFLALLAARRSPRTVEAYRRDLADFAR
                     FRGGSPATATGEDVQDWLTDLRARGLAPSSIARKASAARTFYRHLVLLGARADNPAAE
                     VPLPRKRTKLPRSLTPGEVERLIEAANGVHPRNLRDRALVELLYGAGLRVSEAVGLER
                     GSVDLEERIVRCVGKGDKERLVPLGREAVEAVRRYLSRGRPYLDRRHRRELFLNAQGG
                     ALTRAGAFFVLRRVALKAGLDPGRVHPHLLRHSFATHLLEGGADLRSVQEMLGHADLG
                     TTEIYTHVSEKRRRDTYFSSHPHARRR"
     gene            1567..>3012
                     /locus_tag="VFO88_11005"
     CDS             1567..>3012
                     /locus_tag="VFO88_11005"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET9546093.1"
                     /translation="MTLDAWITLAVVVVTVGVLAAELVAPSVAVLGGVAALLVLGVID
                     VEQGFAGFSNPAPLTVAALYVVAAAVRKTGVLERIVGAVLSSGERAGGERSGLARILT
                     PVAGSSAFLNNTPIVAMVAPAVVSWCRRTGQSPSRYLLPISFATILGGTLTLVGTSTN
                     LVVSGLLREAGEEPLGLFEIGKVGLPVAIAGIALLVVVTPVLLPRRRAPSESLDMDAR
                     EFTVEMIVAGNAPPAGQTVSDAGLRNLQGVYLVEIERDGHRIAPVGPDEELSAGDRLV
                     FAGNVDRILDLQSRPGLVSAEEPHFAAVGSTIHRRLFEAVVAGAGALDGATLKDVGFR
                     SRFGAAVLAVHRAGERMPGKLGGVRLRAGDVLLLLADVGARPRLLADPSFLTVAPLAA
                     EAPPRREKGPLGGILIVAMLVLAGTGVLDILVAAMLAAFALVVLRVLSAAEARNAIDL
                     NIIVLIAASFGLGNAIEASGLASQVAGRVVGP"
BASE COUNT          337 a         1163 c         1105 g          407 t
ORIGIN      
        1 ggccggggcg cgacccgcgc aagctcgccc cgaccacgct cgcgcgcaag ctcgcggccg
       61 tgcgctcgtt cctccgcgcc tcgctcgggc ccgcgcacgt gcccgacctc tcgctcgggg
      121 ggaagcggcg gcggcgcctc cctcacgcgc cgcgaccgga cgagacgaac gcgctcctcg
      181 acgggttcga gcccgcgacc ccgctcggcc tgcgaaaccg cgcgctcttc gagctcgtct
      241 actccgccgg cctgcgcgcc caggaggcgt gcgacctcga gctcgcggac gtcgacttcg
      301 aggccgaggc cgtccacgtc cgcgggaagg gcggcaagga gcgcgtcgtc ccgctcggcg
      361 aggaggccgc ctaccggctc gggctctacc tcgagcgcgg ccgcccggcg ctcgcgcgcg
      421 gcgccgtgaa cgcgctcttc ctctccacgc gcggccggcc gctcgagacc tcgacgctcc
      481 gccgcctgct cccgaacccg caccggctgc gccacgcgtt cgccaccgac ctcatggaag
      541 gcggcgcgga cctgaggacg atccaggagc tcatgggcca ctcctcgctc tccacgaccc
      601 agatctacag ccacgtcgac gcacggcggc tgcggaggat ctatgaccga gcgcaccccc
      661 gtgcctgatc cggcgccgga cccgcaggtc gaggggttcc tcgcgctcct cgccgcgcgc
      721 cgctcgccgc gcaccgtcga ggcgtaccgc cgcgacctcg cggacttcgc ccggttccgg
      781 ggcggctcgc cggcgaccgc gaccggcgag gacgtccagg actggctcac cgacctgcgc
      841 gcccgcgggc tcgcgccgag ctcgatcgcc cgcaaggcgt cggcggcgcg gacgttctac
      901 cgccacctcg tcctgctcgg tgcacgcgcg gacaacccgg ccgccgaggt cccgctcccg
      961 cgcaagcgga cgaagctgcc gcgctcgctc acgccgggcg aggtcgagcg gctgatcgag
     1021 gcggcgaacg gcgtccaccc gcgcaacctc cgcgaccgcg cgctcgtcga gctgctctac
     1081 ggcgcgggcc tgcgggtcag cgaggcggtc gggctcgagc gcgggagcgt cgacctcgag
     1141 gagcggatcg tccgctgcgt cgggaagggc gacaaggaac ggctcgtccc gctcggccgc
     1201 gaggcggtcg aggcggtgcg gcgctacctc tcgcgcgggc ggccgtacct cgaccgccgc
     1261 catcgccggg agctcttcct gaacgcacag ggcggcgcgc tcacgcgggc gggggcgttc
     1321 ttcgtcctcc ggcgggtcgc gctcaaggcg gggctcgacc ccggccgcgt gcacccgcac
     1381 ctcctgcggc actcgttcgc cacgcacctg ctcgagggcg gcgcagacct gcggagcgtt
     1441 caggagatgc tcggccacgc cgatctcggg acgaccgaga tctacacgca cgtctcggag
     1501 aagcgccgcc gggacacgta cttctcctcg cacccgcacg ctcgtcgcag gtagaacctg
     1561 cgcgtcgtga cgctggacgc ctggatcacg ctcgcggtcg tcgtcgtcac cgtcggcgtg
     1621 ctcgcggccg agctcgtggc gccgtcggtc gcggtgctcg gcggcgtcgc ggcgctcctc
     1681 gtcctcgggg tgatcgacgt ggagcagggc ttcgcgggct tctccaaccc ggcgccgctc
     1741 acggtcgcgg cgctctacgt cgtcgcggcg gcggtgcgga agacgggcgt cctcgagcgg
     1801 atcgtgggcg ccgtgctctc gagcggcgag cgcgcgggcg gggagcgcag cggcctcgcg
     1861 cggatcctca cgccggtcgc gggatcgtcg gcgttcctga acaacacgcc gatcgtggcg
     1921 atggtcgcgc ccgccgtcgt ctcctggtgc cggcgaacgg gccagtcgcc gtcgcgctac
     1981 ctcctgccga tctcgttcgc gacgatcctg ggcggcacgc tcacgctcgt cgggacgtcg
     2041 acgaacctcg tcgtctcggg cctcctgcgc gaggccggcg aggagccgct cggcctcttc
     2101 gagatcggga aggtcggctt gccggtcgcg atcgcgggga tcgccctgct cgtcgtcgtc
     2161 accccggtgc tcctcccccg gcggcgcgca ccgagcgagt cgctcgacat ggacgcgcgc
     2221 gagttcacgg tcgagatgat cgtcgccggc aacgcgccgc cggccgggca gaccgtctcg
     2281 gacgcggggc tccggaacct ccagggcgtc tacctcgtcg agatcgagcg cgacggccac
     2341 cggatcgcgc cggtcgggcc ggacgaggag ctctccgccg gcgaccggct cgtcttcgcg
     2401 gggaacgtcg accggatcct cgacctccag tcccggcccg ggctcgtctc ggcggaggag
     2461 ccgcacttcg ccgccgtcgg ctcgacgatc caccggcggc tcttcgaggc ggtcgtcgcc
     2521 ggggcgggcg cgctcgacgg cgcgacgctc aaggacgtcg ggttccggag ccgcttcggc
     2581 gccgcggtgc tcgccgtgca ccgggcgggt gagcggatgc cgggaaagct cggcggcgtg
     2641 cgcctgcggg ccggcgacgt gctgctcctc ctggccgacg tcggggcgcg gccgcggctc
     2701 ctcgccgacc cgagcttcct caccgtcgcg cccctcgccg ccgaggcgcc gccgcggcgc
     2761 gagaaggggc cgctcggcgg gatcctgatc gtggcgatgc tcgtcctcgc cgggacgggc
     2821 gtgctcgaca tcctcgtcgc ggcgatgctc gcggccttcg cgctcgtcgt gctgcgggtg
     2881 ctctcggcgg cggaggcgcg gaacgcgatc gacctgaaca tcatcgtcct catcgcggcg
     2941 agcttcgggc tcgggaacgc gatcgaggcg agcgggctcg cgtcgcaggt cgcgggccgc
     3001 gtggtcgggc cg
//
LOCUS       DASTFC010000375         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_376459,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000375 DASTFC010000000
VERSION     DASTFC010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376459"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFO88_11010"
     CDS             <1..519
                     /locus_tag="VFO88_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546094.1"
                     /translation="NDMRRLWEDHIVWTRLAIVSLTTDAPDTEATVGRLLRNQDEIGA
                     AIAPFYGEAAGDELARLLRDHIVIAAGLVAAARAGDAEAVADAQARWGANADEIAAFL
                     AAANPRAWDEAVLRASLEEHLRMTTDEAVARLTGDWEGDVAAYDAIHAHILGFADVLS
                     TGIVRQFPGRFR"
     gene            558..1220
                     /locus_tag="VFO88_11015"
     CDS             558..1220
                     /locus_tag="VFO88_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9546095.1"
                     /translation="MNGHATNARTAAPLLDAESRAWVRALRAGEPDREEAIARLHALL
                     LRAARFEVARRRPGLPHLRGGELDDIALEAADDAVVSVLRRLDTFRGLSRFTTWAYKF
                     ALLEAAVKLRRRAWQGRELPLEPESWALFAADGLAPEAEAEQRELLDVIQTAIAELLT
                     PHQRRVLVALAVNGVPIDVLAERTGTTRGALYKALHDARRKLRRHLAERGLPLDDMKE
                     AS"
     gene            1220..1453
                     /locus_tag="VFO88_11020"
     CDS             1220..1453
                     /locus_tag="VFO88_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546096.1"
                     /translation="MDRPDRKQALGRLLGPAAPEIGCDECFEELDRYVELELAGADAD
                     AAVPGLRAHLDGCPACREEHESLRALVGGDQAL"
     gene            1491..>2644
                     /locus_tag="VFO88_11025"
     CDS             1491..>2644
                     /locus_tag="VFO88_11025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9546097.1"
                     /translation="MEAPRLNRSLWLAESEPGYPALEGDADDVVDAVVVGAGITGATT
                     AFLLAEAGASVALLDSSRVAHGATGYTTAKLTVGHNIVYRTLVDSYGEEVARRFARSN
                     GEAIERVAGLVARHALDCDFERATNYVYAERPESVPELEEEAEAARAAGVAAELTTVT
                     DLPYPVAAALRVDGQAQFHPWKYVAGLVRLVAERGGRVYELTRATHVAKGDPCVVTTP
                     RGRVRGRHVVLATQLPFLDRGLFFARAHPVKSYAVAAPVPDDVAPRGMYISCDAPTRS
                     VRSAPGPDGARFVIVGGEGHKPGADPATEERYARLEEFLAERFGGAQAEYRWSTHDYV
                     PVDHLPYIGRLRGGDPRILVATGFAKWGMTKGTLAASILADSVLGRPNEYA"
BASE COUNT          332 a          971 c         1021 g          320 t
ORIGIN      
        1 aacgacatgc gccgcctgtg ggaggaccac atcgtctgga cgcggctcgc gatcgtgagc
       61 ctgacgaccg acgcgcccga cacggaggcg accgtcgggc ggctgctccg caaccaggac
      121 gagatcggcg cggcgatcgc gcccttctac ggcgaggcgg cgggcgacga gctcgcgcgc
      181 ctgctgcgcg accacatcgt catcgcggcc gggctcgtcg ctgccgcgcg ggcgggcgac
      241 gcggaggcgg tcgccgacgc gcaggcccgc tggggcgcga acgccgacga gatcgcggcg
      301 ttcctcgcgg ccgcgaaccc gcgtgcctgg gacgaggccg tcctgcgggc gtcgctcgag
      361 gagcatctcc ggatgacgac ggacgaggcg gtcgctcggc tcacgggcga ctgggagggc
      421 gacgtggccg cgtacgacgc gatccacgcg cacatcctcg gcttcgcgga cgtgctctcg
      481 accgggatcg tccgtcagtt cccggggcgg ttccgctagc cgtggttgcc gccgccgcta
      541 ccgttcggag cgggctcgtg aacgggcacg cgacgaacgc gcgaacggcg gcgccgctcc
      601 tcgacgcgga gtcgcgcgcc tgggtgcgcg cgctccgcgc cggggagccc gatcgggagg
      661 aagcgatcgc gcggctgcac gcgctcctcc tgcgggcggc gcgcttcgag gtggcgcggc
      721 ggcggccggg gctcccgcac ctgcgcggcg gcgagctgga cgacatcgcg ctcgaggccg
      781 ccgacgacgc ggtcgtgagc gtcctccggc ggctggacac gttccgcggg ctcagccggt
      841 tcacgacgtg ggcgtacaag ttcgccctcc tcgaggcggc cgtgaagctc cgccggcgcg
      901 cctggcaggg ccgggagctc ccgctcgagc cggagtcgtg ggcgctcttc gccgccgacg
      961 gcctcgcgcc cgaggcggag gcggagcagc gcgagctcct cgacgtgatc cagacggcga
     1021 tcgccgagct cctgacgccg caccagcgcc gcgtcctcgt ggcgctcgcc gtgaacggcg
     1081 tgccgatcga cgtgctcgcg gagcggacgg gcacgacgcg cggcgcgctc tacaaggcgc
     1141 ttcacgacgc gcgccgcaag ctccgccggc acctcgcgga gcgcgggctc ccgctcgacg
     1201 acatgaagga ggcgagctga tggaccggcc ggacaggaag caggcgctcg gacggctcct
     1261 cgggccggcc gcgccggaga tcggctgcga cgaatgcttc gaggagctcg accgctacgt
     1321 cgagctcgag ctcgccgggg ccgacgcgga cgcggcggtc cccgggctcc gggcccacct
     1381 cgacggctgc ccggcctgcc gcgaggagca cgagagcctc cgcgcgctcg tgggcggcga
     1441 ccaggcgctc tagacgtgtc gcgggcgagc cgccggggca ggcagtgacg atggaggcgc
     1501 cgcggctgaa ccggtcgctc tggctcgccg agtccgaacc gggctacccc gcgctcgaag
     1561 gcgatgccga cgacgtggtc gacgctgtcg tcgtcggcgc ggggatcacg ggcgcgacga
     1621 ccgcgttcct cctcgccgag gccggtgcga gcgtcgcgct cctcgactcg agccgggtcg
     1681 cgcacggggc gacgggctac acgaccgcga agctcaccgt cggccacaac atcgtctacc
     1741 ggacgctcgt cgactcgtac ggcgaggagg tcgctcggcg cttcgcgcgc tcgaacgggg
     1801 aggcgatcga acgggttgcc ggcctcgtcg cccggcacgc gctcgactgc gacttcgagc
     1861 gcgcgacgaa ctacgtgtac gcggagcggc ccgagtcggt ccccgagctc gaggaggagg
     1921 cggaggccgc gcgcgcggcc ggcgtcgcgg cggaactcac gacggtcacg gacctcccgt
     1981 atcccgtcgc ggcggcgctc cgggtggacg gccaggcgca gttccacccc tggaagtacg
     2041 tcgcagggct cgtccggctc gtcgccgagc ggggcgggcg ggtgtacgag ctcacgcgcg
     2101 cgacgcacgt cgcgaagggc gacccgtgcg tcgtcacgac gccgcgcggg cgcgtgcgcg
     2161 gccgccacgt cgtcctcgcg acgcagcttc cgttcctcga ccgcgggctc ttcttcgcgc
     2221 gggcgcaccc ggtgaagtcg tacgcggtcg ccgcgcccgt gcccgacgac gtggcgccgc
     2281 gcgggatgta catctcgtgc gacgcgccga cgcgctccgt ccgctccgcg ccggggccgg
     2341 acggcgcgcg cttcgtgatc gtcggcggcg aggggcacaa gccgggcgcc gacccggcca
     2401 cggaggagcg ctacgcccgg ctcgaggagt tcctcgccga gcggttcggc ggcgcgcagg
     2461 ccgagtaccg ctggtcgacg cacgactacg tgccggtcga ccacctcccg tacatcgggc
     2521 gcctccgggg cggcgacccg cgcatcctcg tcgcgaccgg gttcgcgaag tgggggatga
     2581 cgaaggggac gctcgccgcc tcgatcctcg cggactcggt tctcgggcgc ccgaacgagt
     2641 acgc
//
LOCUS       DASTFC010000376         3816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_179175,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000376 DASTFC010000000
VERSION     DASTFC010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179175"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFO88_11030"
     CDS             <1..503
                     /locus_tag="VFO88_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546098.1"
                     /translation="NGIDVLDAARHKAETGLLGGYSEAEDVTNAELLLLPCDVLAPCA
                     LEQEITADNADGVQASIVCEGANGPVTIEADDILADRGVLVLPDVLANAGGVVVSYFE
                     WVQGLQEYFWKEEEVNERLNEIVTRAFDQTWTLHETRSVTMRQAAYLLAVGRVAEATI
                     TRGLYP"
     gene            505..777
                     /locus_tag="VFO88_11035"
     CDS             505..777
                     /locus_tag="VFO88_11035"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET9546099.1"
                     /translation="MDGGAPRVVLYAAPGCHLCAAARRVLDGVRADTPFELEEVDISA
                     SPELERRYRERIPVVEVGGEEAFTYFVHPDGLRRRLAQSPPPSRSS"
     gene            835..1497
                     /locus_tag="VFO88_11040"
     CDS             835..1497
                     /locus_tag="VFO88_11040"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET9546100.1"
                     /translation="MSRLAELSTETERAGGDRLTVGVAARLSQYLRVLTQARKVGKDR
                     ISSQEISEYTNINATQIRRDLSGFGKFGKRGVGYNVDALSGEIRKILRTQGQHNIALF
                     GVGRLGQAVANSSVFADHGFNIAAAFDTDPAKVGRTEDGLEVLGYDRLENVIREKNII
                     VAVLAVPAEAAQSVADDVVAAGVKIIFNYTDALLDVPSDVTVHTTSPAVELLYALYFY
                     LT"
     gene            complement(1512..2174)
                     /locus_tag="VFO88_11045"
     CDS             complement(1512..2174)
                     /locus_tag="VFO88_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546101.1"
                     /translation="MAERHPDELDLLDLVEGELPRAAQGSVAAHVEGCARCSEVVRQL
                     RAGRGALHSSGVLQLSERARDRISAAIDEEPRARRFRFWEPRRLGLVVAATLVLVASA
                     VAVAQWGGVGDEQGGDAASELAEDDGGGGDGAEGGGVEEDAQTVVPESTTDTIRVAGP
                     AREVAAELRAEGFEARVVRGEVVVELTGERLDEGQAPEEDRLQAVLAERGPGRVTVRV
                     ER"
     gene            complement(2174..2719)
                     /locus_tag="VFO88_11050"
     CDS             complement(2174..2719)
                     /locus_tag="VFO88_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9546102.1"
                     /translation="MPQDPDAPLVRRAQRGDRLAFERLVDRHQHRLFTLAARTLGDRS
                     EAEDAVQEAFLRAWRGLGSFRGGSLFSTWLYRICVNAAHDQRERRRAEPVETVDTAPD
                     ARDRFAERELSGELQRALAALDEEQRRTVVLSDVLGCSYAEIAEITGVAEGTVKSRLF
                     RARLELARLLGTGGGGAPSNE"
     gene            2722..>3816
                     /locus_tag="VFO88_11055"
     CDS             2722..>3816
                     /locus_tag="VFO88_11055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9546103.1"
                     /translation="MKVLLWHGYLLGGTGSNVYTRALARAWAHLGHEVVVFCQDPHPE
                     RFDLGGATVVRPDLRGGPLPVFVLDRYEDVDARYLGELSWPERREFVERNAAAVREHL
                     PADFLLVNHVLLGAPVGAASGMPFGVKAHGSELEFSIRGNEPLADWAHESLDEARAVY
                     AGSEHIRRVLEEVVGPGEYSRRVHVVPPGVDVDALRPRPRDEALAALLEEARRDPPNP
                     GNARERLPDEGNAARLEAHLAGEGPTVVYVGKLSREKGVELFLEAVGRLGVRAIVVGF
                     GPERAALEARAGPTVLFTGPLEHRHLGPLWALADVSVTPWVFPEAFGMVAAEAAACGS
                     PPLVARHSGLAEIAEALEEEYPAELRHLTSF"
BASE COUNT          494 a         1456 c         1363 g          503 t
ORIGIN      
        1 cgaacgggat cgacgtgctc gacgccgcgc gccacaaggc ggagaccggt ttgctcggcg
       61 gctactccga ggcggaggac gtcacgaacg ccgagctgct cctcctgccg tgcgacgtgc
      121 tcgctccgtg cgcgctcgag caggagatca ccgccgacaa cgcggacggc gtccaggcct
      181 cgatcgtctg cgagggcgcg aacggcccgg tcacgatcga ggcggacgac atcctggccg
      241 accgcggggt cctcgtcctc ccggacgtgc tcgcgaacgc gggcggcgtc gtcgtctcgt
      301 acttcgagtg ggtgcagggc ctccaggagt acttctggaa ggaggaggag gtgaacgagc
      361 ggctgaacga gatcgtcacg cgcgcgttcg accagacgtg gacgctgcac gagacccgct
      421 cggtcacgat gcgccaggcc gcgtacctcc tcgccgtcgg ccgcgtggcc gaggcgacga
      481 tcacccgcgg gctctacccc tagcgtggac gggggcgcgc cgcgcgtcgt cctctatgcg
      541 gcgcccggct gccacctctg cgccgccgcg cgtcgcgtgc tcgatggcgt gcgggcggac
      601 acgccgttcg agctcgagga ggtggacatc tccgcctcgc ccgagctcga gcgccgctac
      661 cgcgagcgga tcccggtcgt cgaggtcggc ggggaggagg cgttcacgta cttcgtccac
      721 ccggacggcc ttcgccggcg gctcgcacaa agtccgccgc cgagccggag ttcgtgacag
      781 cgcaggttct ctccacgcct gcagttccgc gcgggctatg ctccgtcaca agcgatgtcg
      841 cggctggccg aactcagcac ggagaccgag cgggcgggag gtgaccgcct cacggtcggc
      901 gtcgcggcgc gcctgagcca gtacctccgc gtcctcacgc aggcgcgcaa ggtcggcaag
      961 gaccggatct cctcccagga gatcagcgag tacacgaaca tcaacgcgac gcagatccgc
     1021 cgcgacctct cgggcttcgg gaagttcggc aagcgcggcg tcggctacaa cgtcgacgcg
     1081 ctctccggcg agatccgcaa gatcctgcgg acgcaggggc agcacaacat cgcgctcttc
     1141 ggcgtcgggc ggctcgggca ggcggtcgcg aactcgtccg tcttcgccga ccacggcttc
     1201 aacatcgcgg ccgcgttcga caccgacccc gcgaaggtcg gccgcaccga ggacggcctc
     1261 gaggtgctcg gctacgaccg gctcgagaac gtcatccgcg agaagaacat catcgtcgcc
     1321 gtgctcgccg tcccggccga ggccgcgcag agcgtcgcgg acgacgtcgt cgcggccggc
     1381 gtgaagatca tcttcaacta cacggacgcg ctcctcgacg tcccctcgga cgtcaccgtc
     1441 cacacgacga gcccggccgt cgagctgctc tacgcgctct acttctacct gacgtagccc
     1501 gccggagcgg gttaccgctc gacgcggact gtgacgcggc cggggccccg ctcggcgagg
     1561 acggcctgga ggcggtcctc ctccggcgcc tgcccctcgt ccagccgctc gccggtcagc
     1621 tcgacgacca cctcgccgcg cacgacgcgc gcctcgaagc cctcggcgcg cagctcggcg
     1681 gccacctcgc gggcgggccc cgcgacacgg atcgtgtccg tcgtcgactc cggcacgacc
     1741 gtctgcgcgt cctcctccac gcctccgccc tctgcgccgt cgccgccgcc gccgtcgtcc
     1801 tcggcgagct cgctcgcggc gtcgccgccc tgctcgtcgc cgacgccgcc ccactgggcg
     1861 accgcgaccg cggacgcgac gagcacgagg gtcgccgcga ccacgaggcc gagccggcgc
     1921 ggctcccaga accggaaccg gcgcgcgcgc ggctcctcgt cgatcgccgc ggagatcctg
     1981 tcgcgcgcgc gctcggagag ctggaggacg ccggacgagt gcagcgcccc ccgcccggcg
     2041 cgcagctgcc gcacgacctc ggagcaccgt gcgcagccct cgacgtgcgc ggcgaccgat
     2101 ccctgcgccg cgcgcgggag ctcgccctcg acgaggtcga gcaggtccag ctcgtctggg
     2161 tggcgttcgg ccatcactcg ttggacgggg cgccccctcc gccggttccg aggagccgcg
     2221 cgagctcgag ccgcgcccgg aagagacgcg acttcaccgt accctcggcg acgcccgtga
     2281 tctccgcgat ctccgcgtac gagcagccga gcacgtcgga gaggacgacc gtgcgccgct
     2341 gctcctcgtc gagcgccgcg agcgcgcgct ggagctcgcc cgagagctcc cgctcggcga
     2401 agcggtcgcg cgcgtcgggt gccgtgtcga ccgtctcgac cggctcggcg cgccgccgct
     2461 cgcgctggtc gtgggccgcg ttgacacaga tccggtagag ccaggtcgag aagagcgagc
     2521 ccccgcggaa cgagcccagg ccgcgccagg cgcggaggaa cgcctcctgc accgcgtcct
     2581 ccgcctccga gcggtcgccg agcgtccgcg cggcgagcgt gaagagccgg tgctggtgcc
     2641 ggtcgacgag ccgctcgaag gcgagccggt cgccacgctg cgcgcggcgg acgagcggtg
     2701 cgtcaggatc ctggggcacc cgtgaaggtt ctcctctggc atggctacct ccttggagga
     2761 accggctcca acgtctacac gcgcgcgctc gcccgcgcct gggcgcacct cgggcacgag
     2821 gtggtcgtct tctgccagga cccgcatccg gagcgcttcg atctcggcgg ggcgaccgtc
     2881 gtccggccgg accttcgcgg cgggccgctg cccgtcttcg tgctcgaccg ctacgaggac
     2941 gtcgacgcgc gctacctcgg ggagctctcg tggcccgagc ggcgcgagtt cgtcgagcgg
     3001 aacgccgccg ccgtgcgcga gcacctgccg gccgacttcc tgctcgtgaa ccacgtcctg
     3061 ctcggcgcgc ccgtcggcgc cgcgtccggg atgccgttcg gggtcaaggc gcacggctcc
     3121 gagctcgagt tctcgatccg cgggaacgag ccgctcgccg actgggcgca cgagtcgctc
     3181 gacgaggcgc gggcggtcta cgcgggctcc gagcacatcc gccgcgtgct cgaggaggtc
     3241 gtcgggccgg gcgagtacag ccggcgcgtc cacgtcgtgc cgcccggcgt cgacgtcgac
     3301 gcgctccgcc cccgcccgcg cgacgaggcg ctcgccgcgc tcctcgagga ggcccgccgc
     3361 gacccgccga acccgggcaa cgcccgcgag cggctccccg acgagggcaa cgccgcgcgg
     3421 ctcgaggcgc atctcgcggg cgaggggccg accgtcgtct acgtcgggaa gctctcgcgc
     3481 gagaagggcg tcgagctctt cctcgaggcg gtcggcaggc tcggtgtgcg cgcgatcgtc
     3541 gtcgggttcg gcccggagcg cgcggcgctc gaggcgcggg ccgggccgac cgttctcttc
     3601 acggggccgc tcgagcaccg ccacctcggc ccgctctggg cgctcgccga cgtgagcgtg
     3661 acgccgtggg tcttccccga ggcgttcggg atggtcgccg ccgaggccgc cgcgtgcggc
     3721 tcgccgccgc tcgtggcccg tcactcgggc ctcgcggaga tcgccgaggc gctcgaggag
     3781 gagtacccgg cggagctccg ccacctcacg agcttc
//
LOCUS       DASTFC010000377         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_409869,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000377 DASTFC010000000
VERSION     DASTFC010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409869"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFO88_11060"
     CDS             <1..323
                     /locus_tag="VFO88_11060"
                     /inference="COORDINATES: protein motif:HMM:NF021902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546104.1"
                     /translation="STSGDPRERTPADNQTVRTVFVIAPDKTIKLMLAYPMTTGRNFE
                     EILRVLDSLRLTAEHRLATPANWRRGEDVIISSAVSDEEAATLFPGYRRVLPYLRVTA
                     PPSS"
     gene            complement(390..1172)
                     /locus_tag="VFO88_11065"
     CDS             complement(390..1172)
                     /locus_tag="VFO88_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546105.1"
                     /translation="MALAAAGLLAGCGGSAGERPGDSAAADLPQGSEPVKLDPAAFTT
                     TIDNRYWPMAPGTRWTYREVDENGEELVVVVTASSETRTIANGVTARVVRDTVTRDGE
                     LVEDTVDWYAQDARGNVWYLGEDTAELDGGSVTTRAGSWEAGVDDALPGVIVPADPVD
                     GMAYRQEHDEGEAEDAGEVLSVAEQAEVPAGHFRDALLTKDTTALEPDVLEYKLYAPG
                     VGPVLVVGVSGGGGREELLRVEQVGEAAARAAGTTPLGEPYP"
     gene            complement(1222..1545)
                     /locus_tag="VFO88_11070"
     CDS             complement(1222..1545)
                     /locus_tag="VFO88_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546106.1"
                     /translation="MKRPTNRRLALLGAAVVVLGAAGGGAALASGGDDDATDTPITGS
                     ALERARAAALAHTGGGEVTGTEVGDEESLYEVEITLDDGSQVDVQLDEGFEVVGSERD
                     GADEE"
     gene            1662..2336
                     /locus_tag="VFO88_11075"
     CDS             1662..2336
                     /locus_tag="VFO88_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9546107.1"
                     /translation="MRVLVVEDEAKLAGLLRRGLEEEGHAVDLARSGDDALWRAAAAE
                     YDAVVLDVMLPGPDGFDVCRRLRERGVWVPVLMLTARDAVADRVAGLDAGADDYLAKP
                     FSFAELLARLRALARRGAVERPAVLEVGDLRLDPAARRVWRGDVEVPVSAKEFCVLET
                     LMRRPGRVLSREELIEHAWDAAYEHRSNVVDVYVRYLREKIDRPFGRRSLETVRGVGY
                     RLRGDL"
     gene            2333..>3322
                     /locus_tag="VFO88_11080"
     CDS             2333..>3322
                     /locus_tag="VFO88_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9546108.1"
                     /translation="MSARIPLRLRLTLVFVVAMATVLAAVGAFLYVRVGDSLLEQVDE
                     SLSARAGELAVSAREGTTGSALAGGDDEFAQVLGPGGRVEAATPGYGGPLLPADELAD
                     ARGGEVRVNARVAPPGEGDREPARLLAFPAGGSIVVVGASLEDRADALSGLLAQLLVG
                     GPLALALAAAGGYVLAGAALRPVEAMRRRAAEISAETTGERLPLPAARDEIHRLGTTL
                     NAMLGRLEAGLVRERRFVADASHELRTPLASLRAELELALRRPRTAGELTAALRSAAE
                     EVDRLVLLAEDLLVLAATEDGDLPVRGAPVDARELMEEVADRFAARAQAAGREL"
BASE COUNT          359 a         1269 c         1198 g          496 t
ORIGIN      
        1 agtcgacgag cggcgacccg cgcgagcgca ccccggccga caaccagacc gtccgcacgg
       61 tgttcgtgat cgcaccggac aagacgatca agctcatgct cgcctatccg atgacgaccg
      121 ggcggaactt cgaggagatc ctccgcgtgc tcgactcgct ccggctcacg gcggagcacc
      181 gcctcgccac gccggcgaac tggcgccgcg gggaggacgt gatcatctcg tcggcggtct
      241 ccgacgagga ggcggcgacg ctcttcccgg gctaccggcg ggtgctgccg tacctccgcg
      301 tcacggcgcc gccctcctcc tgagcggcgc gctgggccgg gggtaccgcc ccggctcccg
      361 gctcagcctc cgcaacgtgc tgaacggcgc tacgggtacg gctcgccgag cggcgtcgtg
      421 ccggccgcgc gagcggctgc ctcgcccacc tgctcgacgc ggaggagctc ctcgcggccg
      481 ccgccgcctg agacgccgac cacgagcacc gggccgacgc ccggcgcgta gagcttgtac
      541 tcgagcacgt ccggctcgag cgcggtcgtg tccttcgtca ggagtgcgtc gcggaagtgg
      601 ccggctggta cctcggcctg ctcggcgacg ctcagcacct cgcccgcgtc ctccgcctcg
      661 ccctcgtcgt gctcctgccg gtaggccatc ccgtcgaccg gatccgccgg gacgatcact
      721 cccggaaggg cgtcgtcgac tcccgcctcc cacgagcccg cccgcgtggt caccgagcct
      781 ccgtcgagct ctgccgtgtc ctcgcccaga taccagacgt tgccgcgcgc gtcctgtgcg
      841 taccagtcca ccgtgtcctc gacgagctcc ccgtcccgcg tgaccgtgtc gcgcacgacg
      901 cgcgccgtga cgccgttcgc gatcgtccgg gtctcgctgc tggccgtaac cacgaccacg
      961 agctcttcgc cgttttcgtc gacctcccgg tacgtccagc gcgtgccggg cgccatgggc
     1021 cagtagcggt tgtcgatcgt cgtcgtaaag gccgccgggt cgagcttcac gggctcggat
     1081 ccctgcggga gatcggcggc cgcggagtcg cccggccgtt cgccggcgga tcctccgcag
     1141 cccgcaagca ggccggcggc ggcgagcgcc actgctcgtg cacgtggggg cacctgcgcc
     1201 tcccgccggc cggccgctcc gctactcctc gtccgcgccg tcgcgctcgc tgccgaccac
     1261 ctcgaagccc tcgtccagct gaacgtcgac ctggctcccg tcgtcgagcg tgatctcgac
     1321 ctcgtagagg ctctcttcgt caccgacctc cgtgcccgtc acctcgccgc cgccggtgtg
     1381 ggcgagggcg gccgccctcg cccgctcgag cgcggagccg gtgatgggcg tgtcggtcgc
     1441 gtcgtcgtcg ccgcccgacg cgagcgccgc cccgccgccg gccgcgccga ggacgacgac
     1501 cgccgccccg aggagcgcga gccttctgtt cgtcggccgc ttcatcgtgt gccctccttc
     1561 ccctcgtgat caacgaccac gctagccgtc gcggatgaga gggtcgtgag agaggccggc
     1621 cctctcatcg cgctctcatc tccgtcgcga caatgggccg tatgcgtgtg ctcgtcgtcg
     1681 aggacgaggc gaagctcgcg ggcctgctcc gccgcggcct ggaggaggag ggccacgccg
     1741 tcgacctcgc gaggagcggc gacgacgcgc tctggcgggc cgccgcggcc gagtacgacg
     1801 ccgtcgtgct cgacgtcatg ctgccggggc ccgatgggtt cgacgtctgt cggcggctgc
     1861 gggagcgcgg cgtctgggtg cccgtgctga tgctgaccgc acgcgacgcc gtggccgacc
     1921 gcgtcgcggg cctcgacgcg ggcgccgacg actacctggc gaagccgttc tcgttcgccg
     1981 agctgctcgc gcggctccgg gcgctcgcgc gccgcggggc cgtggagcgg cctgccgtcc
     2041 tcgaggtagg ggatctccgc ctcgatcccg ccgcgcgccg ggtgtggcgc ggcgacgtcg
     2101 aggttcccgt ctcggcgaag gagttttgcg tgctcgagac gctcatgcgc cgcccgggcc
     2161 gtgtcctctc ccgggaggag ctcatcgagc acgcgtggga cgccgcttac gagcaccgtt
     2221 cgaacgtcgt ggacgtctac gtgcgatacc tgcgggagaa gatcgaccgg ccgttcggcc
     2281 gccgctcgct tgaaaccgtg cggggcgtgg gctaccgcct ccggggggat ctgtgagcgc
     2341 gcggatcccg ctccggctcc gcctgacgct cgtgttcgtg gtcgcgatgg ccaccgtcct
     2401 cgctgcggtc ggcgcgttcc tctacgtccg ggtcggagac tcgctcctgg agcaggttga
     2461 cgagagcctc tccgcgcgtg ccggcgagct cgccgtctcg gcgcgcgaag ggacgactgg
     2521 gtcggcgctc gccggcggcg acgacgagtt cgcccaggtg cttggccccg gcggtcgcgt
     2581 ggaggcggcc acccccggct acggcgggcc gctcctcccg gcggacgagc ttgcagacgc
     2641 gcggggcggg gaggttcgcg tgaacgccag ggtggcgcca cccggcgagg gcgatcggga
     2701 gcccgcccgc ctgcttgcgt ttccggccgg cggctcgatc gtcgtcgtgg gcgcctcgct
     2761 cgaggatcgc gcggatgcgc tttccggtct tctcgcgcag ctcctcgtcg gcgggcccct
     2821 cgcgctcgcc ctggcggccg ccgggggtta tgtcctcgcc ggcgccgcgc tgcggccggt
     2881 ggaggcgatg cgacgtcgcg ccgccgagat ctcggccgag acgacgggag agcggctgcc
     2941 cctcccggcg gcccgcgacg agattcaccg gttggggacg acgctgaacg cgatgctcgg
     3001 ccgcctcgag gcgggcctcg tccgcgagcg ccgcttcgtc gccgacgcga gccacgagct
     3061 gcgcacgccg ctcgcgtcgc ttcgcgccga gctcgagctt gcgctccgcc ggccgcgcac
     3121 cgccggtgag ctcacggccg cgctccggtc ggccgccgag gaggtcgacc ggctcgtgct
     3181 cctcgccgaa gacctcctcg tcctcgccgc gacggaggac ggcgaccttc cggtccgcgg
     3241 ggccccggtc gacgcgcggg agctcatgga ggaggtcgcg gaccgcttcg ccgcgcgtgc
     3301 gcaggcggcc gggcgggagc tc
//
LOCUS       DASTFC010000378         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_624339,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000378 DASTFC010000000
VERSION     DASTFC010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624339"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1056)
                     /locus_tag="VFO88_11085"
     CDS             complement(145..1056)
                     /locus_tag="VFO88_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET9546109.1"
                     /translation="MTTRAPTVVALGGNALLRSGERGTAAEQRANLADTFAAIADLLR
                     AESVVLTHGNGPQVGNELLRHELAAAESPPLPLYLAVAQTQAEIGALAVSELEPVTGH
                     AAAVVLTRVAVAADDPAFSDPTKPIGPFYDEGRARELERERGWRLREDAGRGWRRVVP
                     SPRPLEILELESIRALAAAGTTVVAAGGGGVPVVRRDGRYDGVDAVIDKDFASALLAE
                     RLGAERLLILTQVPALYRGFGTPEQEPVEELVAGRDDGLVADLPAGSMRPKVEAAFAF
                     VRATGGEARIASAEGLAEGNFGTRIRT"
     gene            complement(1101..2438)
                     /locus_tag="VFO88_11090"
     CDS             complement(1101..2438)
                     /locus_tag="VFO88_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HET9546110.1"
                     /translation="MRRIVIAGAAGRDFHNFNVAFRDDPAAQVVAFTATQIPDIDGRV
                     YPTELAGGRYPDGIPIVAEDGLADLVRDENVDEVVFAYSDVSHEQVMHLGSIALAAGA
                     DYRLMGPRATEIASSKPVVAICAVRTGSGKSQTTRKVAAVLREAGLRVAVLRHPMPYG
                     DLVRQRVQRFERYEDLDAADCTIEEREEYEPHLAEGNLVFAGVDYEAILRQAESEADV
                     IVWDGGNNDTPFIAPDVHLVVVDPHRAGHERTYHPGETNLRMADVCVVNKMDSAPAEG
                     VEAVLRSIEELNPRAEVVRASSPIGVEGDAAEIRGKRVLAIEDGPTLTHGDMAYGAAV
                     LAAREHGAAELVDPRPFAVGSIAETFRKYPHVAELLPAMGYGRKQMEELRETIARSDA
                     DLVLIGTPIDLRRIVELDKPALRVTYRLEEIGSPTIEDVLRARGILVSEPALT"
     gene            complement(2435..>3530)
                     /locus_tag="VFO88_11095"
     CDS             complement(2435..>3530)
                     /locus_tag="VFO88_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546111.1"
                     /translation="ARVELQEQELNPIYAEVLERTREGARTLGADTYRELYERFGIPL
                     EELGAGCERLLAETEDLYLAVADRLFRDRLGLTLEEATRPDVLRLLRATTWDDGFPAD
                     AMLPALEGTLAGLGIDLRSQRNVELDVEPRPLKSPRAFCAPIEVPGRVVLVIQPMGGP
                     DDWHALFHEAGHTEHYANTAPGLPVEERRLGDNAVTEGWATLFELLVDDPAWLARRLD
                     FGRPEDFAAEAAAIRLYLVRRYAAKLLYELELHGGADLGGARSRYAELMEEATKIPPS
                     DADFLADVDPGFYAQSYLRSWAFEAQMSLFLREEFGTAWFARREAGSLLRELWAEGQR
                     LTADELLAELDGSRLDLEAIVERTKEPLRV"
BASE COUNT          458 a         1296 c         1294 g          482 t
ORIGIN      
        1 ggagcagaag cccaagcgga tcccggtcag cggatccgag cagacggcga tcgagggcaa
       61 gggaacccgg acctcgtaac gccgtcccga tctctcctcc gaaggggcga ggcgttcggc
      121 ctcgcccctc ggcgttaacc gccctcaagt tcggatgcgc gtgccgaagt tcccctcggc
      181 gagcccctcg gcgctcgcga tgcgcgcctc gccgccggtc gcgcggacga aggcgaacgc
      241 ggcctcgacc ttcggccgca tgctcccggc cgggaggtcg gcgacgagcc cgtcgtcgcg
      301 gccggccacg agctcctcga cgggctcctg ctccggcgtg ccgaacccgc ggtagagcgc
      361 gggcacctgc gtgaggatca ggagccgctc cgcgccgagc cgctcggcca ggagagccga
      421 ggcgaagtcc ttgtcgatca ccgcgtcgac gccgtcgtac cgtccgtcgc gccgtacgac
      481 cgggacaccg ccgccgccgg ccgcgacgac cgtcgtcccg gccgccgcga gcgcgcggat
      541 cgactcgagc tccaggatct cgagcgggcg cggcgagggg acgacgcgcc gccagccgcg
      601 tcccgcatcc tcgcggagcc gccagccgcg ctcgcgctcg agctcgcggg cgcgcccctc
      661 gtcgtagaag gggccgatcg gcttcgtcgg gtcggagaag gccggatcgt ccgcggcgac
      721 cgcgacgcgc gtgaggacga ccgcagccgc gtgccccgtg accggctcga gctcggagac
      781 ggcgagcgcg ccgatctccg cctgcgtctg cgcgacggcg aggtacagcg ggagcggggg
      841 cgactcggct gcggcgagct cgtggcgaag cagctcgttg ccgacctgcg gcccgttccc
      901 gtgcgtgagc acgaccgact cggcccggag caggtcggcg atcgccgcga acgtgtcggc
      961 gaggttggcg cgctgctcgg cggccgtccc gcgctcgccc gagcgcagga gcgcgttccc
     1021 gccgagcgcc accaccgtgg gcgcacgggt cgtcatccgc cagggattcc ggccggcggc
     1081 cgctcttcct ctccgcggcc ctacgtgagt gcgggctcgc tcacgaggat gccgcgcgcc
     1141 cgcaggacgt cctcgatcgt cggcgagccg atctcctcca ggcggtacgt gacccgcagg
     1201 gccggcttgt cgagctcgac gatgcggcgg aggtcgatcg gcgtcccgat caggacgagg
     1261 tcggcgtcgg agcgtgcgat cgtctcgcgg agctcctcca tctgcttgcg cccgtagccc
     1321 atcgcgggga gcagctcggc gacgtgcggg tacttgcgga aggtctccgc gatcgagccg
     1381 accgcgaacg gccgcgggtc gacgagctcg gcggccccgt gctcgcgcgc ggcgaggacg
     1441 gcggccccgt aggccatgtc cccgtgcgtg agcgtggggc cgtcctcgat cgcgagcacg
     1501 cgcttgccgc ggatctcggc cgcgtcgccc tcgacgccga tcggagagct cgcccggacg
     1561 acctcggcgc gcgggttcag ctcctcgatc gacctgagca ccgcctcgac gccctccgcc
     1621 ggcgcgctgt ccatcttgtt cacgacgcag acgtcggcca tccggaggtt cgtctcgccg
     1681 gggtggtacg tccgctcgtg gcccgcgcgg tgcgggtcga cgacgacgag gtgcacgtcg
     1741 ggcgcgatga acggcgtgtc gttgttgccg ccgtcccaga cgatcacgtc cgcctcgctc
     1801 tcggcctgac ggaggatcgc ctcgtagtcg acgccggcga agacgaggtt gccctcggcg
     1861 aggtgcggct cgtactcctc ccgctcctcg atcgtgcagt cggccgcgtc gagatcctcg
     1921 tagcgctcga accgctggac gcgctggcgc acgaggtcgc cgtagggcat cgggtgccgc
     1981 agcacggcca ccctgagccc ggcctcgcgg agcacggcgg cgaccttccg cgtcgtctgg
     2041 ctcttccccg agccggtccg cacggcgcag atcgcgacca cgggcttcga ggaggcgatc
     2101 tccgtcgcgc gcgggcccat gagccggtag tcggcgccgg cggcgagcgc gatcgagccg
     2161 aggtgcatga cctgctcgtg cgagacgtcc gagtacgcga agacgacctc gtcgacgttc
     2221 tcgtcccgca cgaggtcggc gaggccgtcc tcggcgacga tcgggatccc gtccgggtag
     2281 cgcccgcccg cgagctccgt cgggtagacg cgcccgtcga tgtccgggat ctgcgtcgcg
     2341 gtgaaggcca cgacctgggc cgccgggtcg tcacggaagg ccacgttgaa gttgtggaag
     2401 tcacggccgg ccgcgccggc gatcacgatc cgtctcatac gcgtaagggc tccttcgttc
     2461 gttcgacgat tgcctcgagg tcgagccgcg agccgtcgag ctcggccagc agctcgtccg
     2521 cggtcaggcg ctggccttcc gcccacagct ctcgcaggag cgagccggcc tcgcggcgcg
     2581 cgaaccacgc cgtgccgaac tcctcccgca ggaacaggct catctgcgcc tcgaacgccc
     2641 acgagcggag atagctctgg gcgtagaaac cggggtcgac gtcggcgaga aagtccgcgt
     2701 ccgacggtgg gatcttcgtc gcctcctcca tcagctccgc gtagcgggag cgggcgccgc
     2761 cgaggtccgc gccgccgtgc agctcgagct cgtagaggag cttcgccgcg taacggcgca
     2821 cgaggtacag gcggatcgcg gccgcctcgg cggcgaagtc ctccgggcgc ccgaagtcga
     2881 gccgccgcgc gagccacgca gggtcgtcca cgaggagctc gaagagcgtc gcccagccct
     2941 cggtcaccgc gttgtcgccg aggcgccgct cctcgaccgg gaggccgggc gcggtgttcg
     3001 cgtagtgctc ggtgtgcccc gcctcgtgga agagcgcgtg ccagtcgtcc gggccgccca
     3061 tcggctggat cacgaggacg acgcggcccg gcacctcgat cggcgcgcag aacgcgcgcg
     3121 gcgacttgag cggacgcggc tcgacgtcca gctcgacgtt ccgctgcgag cggaggtcga
     3181 tcccgagccc cgcgagcgtc ccctcgagcg cagggagcat cgcgtcggcc gggaagccgt
     3241 cgtcccacgt ggtcgcgcgc aggaggcgga ggacgtccgg ccgcgttgcc tcctcgagcg
     3301 tgagcccgag gcggtcgcga aacagccggt cggccaccgc gagatagagg tcctccgtct
     3361 cggccaggag gcgctcgcag ccggcgccga gctcctcgag cgggatgccg aagcgctcgt
     3421 agagctcgcg gtaggtgtcc gcgccgagcg tgcgcgcgcc ctcgcgggtg cgctcgagca
     3481 cctcggcgta gatcgggttc agctcctgct cctggagctc gacgcgcgcc
//
LOCUS       DASTFC010000379        11561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1366255,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000379 DASTFC010000000
VERSION     DASTFC010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11561
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366255"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..443
                     /locus_tag="VFO88_11100"
     CDS             144..443
                     /locus_tag="VFO88_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546112.1"
                     /translation="MVVLRDVPRPPVETADPLDDVLRRVNRLRIAHGGDPLFELPPAC
                     TSWDGGGCVLERAFADLGVAFVDYRFAYGRELTFEHGLGDFVRAFDKGRFPDLRA"
     gene            507..2303
                     /locus_tag="VFO88_11105"
     CDS             507..2303
                     /locus_tag="VFO88_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET9546113.1"
                     /translation="MAVHEAARAAQAAADRTVAGLWRQAVGRDEVFPAFLVQDGERWN
                     PVGWAEAARRVDDLAAGFLALGVGHGDRVAILSRTRIEWTLTDYALASIGAVSIPIYQ
                     TSSRAECAYLLADSGVSVLVCENAEQLAKTRGLGEEVASLRHTVAFGDAEVGALPLEE
                     LVERGRTHVRDDPGALAAARERVAPGDTLTHIYTSGTTGSPKGCVLSHGNFTALTGSV
                     AQIEELFEHGDTVLLFLPLAHNFGRLVQYCAAEIGFTIAYCPEVADLQAAFARVRPTL
                     FPTVPRLFEKAHAGVQASLAEAGAPKRRLADWALHVGSRAAARRAEGRRAGPLLALQA
                     PLADRLVLGKVRERFGGRLRLAVSGGAPLARPIIEFFAGCGVLVLEGYGLSETTSGCT
                     LNRPHDHRFGTVGRAVPGVELAIAPDGEIMVRGPTVFQRYHGRPEATADALTDDGWLL
                     TGDIGTLDDDGFLTITDRKKEIIVTSGGKKIPPQNVENALKASGYISQALLVGEGRPY
                     LVALVSVDRDEAGKVAHGEDAVRALVARVIDEVNSHVGPTEQIKRFALLPRDFSADEG
                     EVTPTLKLKRRVCEEHFADEIERLYSLPKGAA"
     gene            complement(2300..3121)
                     /locus_tag="VFO88_11110"
     CDS             complement(2300..3121)
                     /locus_tag="VFO88_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET9546114.1"
                     /translation="MRAEGWRKVASATWRDPRDPQIYGDLEIDATELLAFVSDIRETT
                     GTRLTVTHLVGRAVARALAENPELNVRKRRGRFVPREGIAIFFVVAVDEGRDLSGVRV
                     RDADAKSAVEIAEELRARAARIRSGEDADFGRSKRVLALTPVPVLGLALRLVTWLTSD
                     LGLDLRRFGLRSHPFGSAMVTSVGMFGVQRAYAPLSPFYRLPFLALVGEVTEKPVVLD
                     GEIVARPILTIAATMDHRYLDGSHAARLAKSVRRYLEDPRAQEPPLAEAPVEAKT"
     gene            3293..3529
                     /locus_tag="VFO88_11115"
     CDS             3293..3529
                     /locus_tag="VFO88_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546115.1"
                     /translation="MLEKGDDMRYGGLHVIATAAVALFALAGCGTSETGGRREASEPA
                     GNGGNDLRIALERARAIERHIEDDASIPRRAGGA"
     gene            3648..4673
                     /locus_tag="VFO88_11120"
     CDS             3648..4673
                     /locus_tag="VFO88_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9546116.1"
                     /translation="MDDLHSFLDAFEAFLRHLAGVELIPLFVGISFHFLRLSARVRGW
                     QNILRAAYPGIRIPYRTVFGSYWAGVAVNAIAPARGGDVVRLYIAKHRLQGTTYPTIM
                     STLVVETLFDFVLATALFLAAVQMGLLPGLGDLPSLPAFDWSFAVEHPAIAALLGSVF
                     VAGLILFATWASRHVVAFKEKVKLGFAILGDWRKFVTQVVSWQALSWVFRILATAFFM
                     NAFGIPVNVETVLAVVVVGGLATTMPFTPGGAGTQQAVIVFALSGWASSSAILSFSVG
                     MQVATAALTVSVGFAAIAIMLGTVRWREHVRRDEDLMRRKGPDGPETPSANGEPAPAP
                     SGRARRA"
     gene            complement(4624..4989)
                     /locus_tag="VFO88_11125"
     CDS             complement(4624..4989)
                     /locus_tag="VFO88_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9546117.1"
                     /translation="MEDAQQRAVLVADDDAALRLLCRVNLELDGYRVLEAETAEEVTR
                     VLDGEDVDVLLLDVHLGADDGLELAKELRASRPELGVALFTGSVERRDAWGEIAAGFL
                     AKPFTLDELAQTVRALAPR"
     gene            5027..5623
                     /locus_tag="VFO88_11130"
     CDS             5027..5623
                     /locus_tag="VFO88_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546118.1"
                     /translation="MERAVRTGERRGYGAAVEGLLEPLGGTLIRLERVAEATPEEIVA
                     EAHEELPGLQYTLHASAELALGLDPPARVGPLHAELVDALGHARDATAEVSAAVVEGD
                     DDLVGKLLPEWRGALFRVRLARLRALERSPLRLPPEDVARVHRGRGEVTPTATTIVAT
                     GLVVAGAVLFTAGAVLASWPLWAAGLALFGGGFVLFRP"
     gene            5683..5835
                     /locus_tag="VFO88_11135"
     CDS             5683..5835
                     /locus_tag="VFO88_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546119.1"
                     /translation="MTALRQALTAGIAWPTPAERRRAWRSFERALDRHLATRPERRLR
                     PIGTRR"
     gene            complement(5810..7072)
                     /gene="gltX"
                     /locus_tag="VFO88_11140"
     CDS             complement(5810..7072)
                     /gene="gltX"
                     /locus_tag="VFO88_11140"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET9546120.1"
                     /translation="MAPRVRFAPSPTGFLHIGGVRTALFNWLFARHEGGEFLLRVENT
                     DRSREVEESVEQIQESLRWLGLDWDGEVAFQLDRLERHQEVARRLLEAGHAYEDEGAI
                     RFRMPDEGKTGWEDVVRGRVEFPNEKLEDVVLLRSDGRPTYNFASPVDDMDDGITHVV
                     RGEDHIANTPKQIRILEALGAPLPTYAHLPNVLGADGRKLSKRHGAVSVEEFRERGYL
                     PEAIVNYLALLGWSLDAETTVIPPEELVRHFTLDRVGASGATFDYQKLEWLNGVYLRD
                     LPPEEYADRLVGYLREQGFEGDEATIRAAAPLCQEKIATLGEFPAFAGFFFGRVEPDP
                     ALVDGSADLLTEAEQTLAEVEPFTAPEIEQALRGLAERLELKPRKAFGPIRAAVTGSN
                     VSPGLFESLELLGREESLARLSAASRSA"
     gene            7093..7959
                     /locus_tag="VFO88_11145"
     CDS             7093..7959
                     /locus_tag="VFO88_11145"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008833 - deoxyribonuclease IV
                     (phage-T4-induced) activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET9546121.1"
                     /translation="MLLGGHMSSSGGIHTAVDRIEAIGGDALQVFTQSPRMWRPTAHP
                     EENLARFRSRREEAGIGSVLAHAVYLINLASPDDELYEKSLTALVSTMEVASAIEADG
                     VVFHVGSHLGAGFEAGMERAAPAIERALEHASEKTWLLLENCAGTGGTIGRSVDELAA
                     LCDRLGGHERLGLCLDSCHLWASGVDVTDSAVLDAALDEIDERIGLDRLRALHVNDSR
                     DTLGANRDRHANIGEGELGERLAVFLGNPRLQGLPAVMEVPGPDGHGPDAGEMRKLRE
                     LHARAVKAYAAA"
     gene            complement(8203..8961)
                     /locus_tag="VFO88_11150"
     CDS             complement(8203..8961)
                     /locus_tag="VFO88_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET9546122.1"
                     /translation="MRAFTKTALAKELDRARGAGWDKACADAEKAARLPAALLLAVAS
                     QETDMNDVVGDGGHGRGLFQIDDRSHRDFLARSGAARAGGKPPVPAAARYAADLLRWN
                     WDFGARSGVPERDRLLFALSAYNAGGGGALAGYREGDSDKRTTGGDYGRSVLARWAVY
                     QELVGVEPPPPLLRRGSRGKRVEELKARLEAWYSKHAPDAWAAFRIEPGRSYGVRVEA
                     VVRDFQTRTGLEVDGVAGPDTQRALARGRKPRSR"
     gene            complement(8977..9387)
                     /locus_tag="VFO88_11155"
     CDS             complement(8977..9387)
                     /locus_tag="VFO88_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546123.1"
                     /translation="MERPLKVISDLPLRPQDETECRRCDVHCDKVVYPAACVEGACPF
                     LYAYEELGRTYVGCMQKVYAVEIDLELLEEAQGAKPGFGAVKACRQPLPMCRAEVSSC
                     YEHRQDRLGCVNPEFFELPQGSPSFRVIARIDAD"
     gene            complement(9496..10221)
                     /locus_tag="VFO88_11160"
     CDS             complement(9496..10221)
                     /locus_tag="VFO88_11160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9546124.1"
                     /translation="MIDAVLFDWGDTLFHFAYDDELLERGWEAGLAAIGREGLPGHDE
                     TAARFRERYLPLLVGTETLEEVEYPGVIREVLDGFGVSVDEEELGRFLEAEHATWQPA
                     RVMGAQTHALLDSLRARGLKLGLVSNAFDPAWLLHEDLERMGLAERLDTAVFSSEVGL
                     RKPHPEIFRRALEALEVAPERALFVGDSRNADIRGAQALGMRTAQALWFRADPDDGEP
                     EPDFEAFTPMDVLNAVRRLRGEA"
     gene            10391..11128
                     /locus_tag="VFO88_11165"
     CDS             10391..11128
                     /locus_tag="VFO88_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9546125.1"
                     /translation="MQADDGLAASAHDVTRVYGEGDTAVEALRGVSLDVGRGELISIM
                     GPSGSGKSTLMHVMAGLDKPTSGDVAIAGTNIAALNDTELTKLRRAHIGFIFQFFNLL
                     PMLTAEENITLPLQLAGGKQDRDWVENVIDKVGLADRRTHRPSELSGGQQQRVAIARA
                     LVSRPTVVFADEPTGNLDSKTGGEILEVLRESVRDYGQTTVMVTHDANAASIGDRTLF
                     LADGLVMKELRASSPHDLLTAMEEVSS"
     gene            11128..>11561
                     /locus_tag="VFO88_11170"
     CDS             11128..>11561
                     /locus_tag="VFO88_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9546126.1"
                     /translation="MVRVALKGLAGRKLRAALTAIAIVLGVAMVSGTYVLTDTIDKAF
                     SAIFEESYANTDAVVRGAGSDISFQGMSGPSAPIEDDLLDDVLALDSVETAAGGIEDE
                     MNTKIIKKDGTAVNTQGSPSLGFGVDPEASEFNPLTLVEGAWA"
BASE COUNT         1549 a         4189 c         4242 g         1581 t
ORIGIN      
        1 ggctcgacgc tcgtctgcgg gctacgggac ggccggctgc tcgcgagcga ggacctcgga
       61 gagacgtggc gcgcgctcga cggcgacggg atcgcgcgga tcgtccagct cgccgccgcc
      121 tgacgcgggc gtacactgat cgcatggtcg tcctccgtga cgtgccgcgc ccgccggtcg
      181 agaccgccga cccgctcgac gacgtgctcc ggcgggtgaa ccgcctccgg atagcgcacg
      241 ggggcgaccc gctgttcgag ctgccgccgg cgtgcacgtc ctgggacggc ggcgggtgcg
      301 tgctcgagcg cgcgttcgcc gacctcggcg tggcgttcgt cgactaccgc ttcgcgtacg
      361 ggcgcgagct cacgttcgag cacggcctcg gcgacttcgt gcgcgcgttc gacaagggcc
      421 gcttccccga cctccgcgcg tagccggctc aacggcgggg cggccgccct ggagtttgtg
      481 cggaacgtgg cgtagtctgg aggcgcgtgg cggtacacga ggcagcccgg gccgcccagg
      541 cggcggccga ccggaccgtt gccggcctct ggcgacaggc ggtcgggcgc gacgaggtgt
      601 tcccggcctt cctcgtgcag gacggcgagc gctggaaccc ggtcggctgg gcggaggcgg
      661 cgcggcgcgt ggacgacctc gccgccggct tcctcgcgct cggcgtcgga cacggggacc
      721 gggtggcgat cctcagccgg acgcggatcg agtggacgct caccgactac gcgctcgcgt
      781 cgatcggcgc cgtcagcatc ccgatctacc agacgtcgtc gcgggcggag tgcgcgtacc
      841 tcctcgccga ctccggcgtg agcgtcctcg tctgcgagaa cgccgagcag ctcgcgaaga
      901 cccgcggcct cggcgaggag gtcgcgagcc tccggcacac ggtcgccttc ggggacgcgg
      961 aggtcggcgc gctcccgctc gaggagctcg tcgagcgcgg ccgcacccac gtccgggacg
     1021 atcccggcgc gctcgccgcc gcgcgcgagc gcgtcgcgcc cggcgacacg ctcacccaca
     1081 tctacacgtc ggggacgacc ggcagcccga agggctgcgt cctctcgcac gggaacttca
     1141 ccgcgctcac cgggtccgtg gcgcagatcg aggagctctt cgagcacggc gacaccgtgc
     1201 tcctcttcct gccgctcgcg cacaacttcg ggcgcctcgt ccagtactgc gcggccgaga
     1261 tcgggttcac gatcgcgtac tgcccggagg tcgccgatct ccaggccgcg ttcgcgcgcg
     1321 tccgccccac gctcttcccg acggtgccgc ggctcttcga gaaggcgcac gcgggcgtgc
     1381 aggcgagcct cgccgaggcg ggcgcgccca agcgacggct cgccgactgg gcgcttcacg
     1441 tcggctcgag agcggcggcg cgccgcgccg aggggcgtcg cgcaggcccg ctgctcgcgc
     1501 tccaggcccc gctcgccgac cggctcgtcc tcgggaaggt ccgcgagcgg ttcggcggcc
     1561 gtctccggct cgccgtctcg ggcggcgcac cgctggcgcg cccgatcatc gagttcttcg
     1621 ccggctgcgg cgttctcgtg ctcgagggct acgggctgag cgagacgacg agcggctgca
     1681 cgctcaaccg cccgcacgac catcgcttcg gcaccgtcgg acgcgcggtt ccgggggtcg
     1741 agctcgcgat cgcgcccgac ggcgagatca tggtgcgcgg gccgacggtc ttccagcgct
     1801 accacggccg cccggaggcg accgccgacg cgctcacgga cgacgggtgg ctcctcaccg
     1861 gcgacatcgg cacgctcgac gacgacggct tcctcacgat caccgaccgc aagaaggaga
     1921 tcatcgtgac gtccggcggc aagaagatcc cgccgcagaa cgtcgagaac gcgctcaagg
     1981 cctcgggcta catctcccag gcgctgctcg tcggggaggg ccggccgtac ctcgtcgccc
     2041 tcgtgtcggt cgaccgcgac gaggccggca aggtcgcgca cggcgaggac gcggtgcgcg
     2101 cgctcgtggc gcgcgtgatc gacgaggtga actctcacgt cgggccgacg gagcagatca
     2161 agcgcttcgc cctgctcccg cgcgacttct cggccgacga gggcgaggtg acgccgacgc
     2221 tcaagctcaa gcgtcgcgtc tgcgaggagc acttcgccga cgagatcgag cggctctact
     2281 cgctgccgaa gggggccgct taggttttcg cctcgaccgg cgcctcggcg agcggcggct
     2341 cctgggcgcg agggtcctcg aggtagcggc ggacgctctt cgcaaggcgc gccgcgtgcg
     2401 acccgtcgag atagcggtgg tccatcgtgg ccgcgatcgt gaggatcggc cgcgcgacga
     2461 tctcgccgtc gaggacgacc ggcttctccg tcacctcgcc gacgagcgcg aggaacggca
     2521 gccggtagaa gggcgagagc ggcgcgtacg cgcgctggac cccgaacatg ccgacggacg
     2581 tcaccatcgc gctcccgaac gggtggctcc ggaggccgaa ccggcgcagg tcgagcccga
     2641 ggtcgctcgt cagccaggtg acgagccgga gcgcgagccc gaggaccggg acgggcgtca
     2701 gcgcgagcac ccgcttcgag cgcccgaagt ccgcgtcctc cccgcttcgg atcctcgccg
     2761 cccgcgcgcg cagctcctcg gcgatctcga cggccgactt cgcgtcggcg tcgcggacgc
     2821 gcacgcccga gaggtcgcgc ccctcgtcga cggcgacgac gaagaagatg gcgatcccct
     2881 cgcgcgggac gaagcggccg cggcgcttgc gcacgttcag ctcggggttc tcggcgagcg
     2941 cgcgggcgac ggcgcggccg acgaggtgcg tgacggtgag ccgggtgccg gtcgtctcac
     3001 ggatgtccga gacgaacgcg agcagctccg tcgcgtcgat ctcgagatcc ccgtagatct
     3061 gcggatcgcg cggatcccgc caggtggccg aggcgacctt ccgccagcct tcagctcgca
     3121 tcaccgctcc tccgggcgcg gtctccgccc gcgtccggtt tcgacggtag cgccgtgccg
     3181 ccacggagtc aacggcgttc cggggctgca cggggacggt cgcgggcgtg cccttaggac
     3241 ggtcgggtga gcgccgatgt ggcgcgcgtg gagggggcct acggtcgccg cagtgctcga
     3301 gaaaggagac gacatgcggt acggcgggct ccacgtcatc gcgacggcgg ccgtcgccct
     3361 cttcgcgctc gccgggtgcg gcacgtcgga aaccggcggc cggagggagg cgtccgagcc
     3421 ggccggaaac ggcggcaacg acctgcggat cgcgctcgag cgggcccggg cgatcgagcg
     3481 tcatatcgag gacgacgcgt cgattccgcg gcgggccggg ggcgcctgac gatcggcggg
     3541 ggcggcccgc gcgaccccgg gaccggctgt gggcgccggc cccgggctcc gcgcgtacgc
     3601 cccgaacgga cggcgcggac gaccacggac agggctactc tcagatcgtg gacgatctcc
     3661 actcgttcct cgacgccttc gaggcgtttc tccgccatct ggcgggcgtc gagctgatcc
     3721 cgctcttcgt cgggatctcg ttccacttcc tccgcctgag cgcgcgcgtg cgcgggtggc
     3781 agaacatcct gcgcgccgcg tatcccggga tccggatccc gtaccggacg gtcttcggct
     3841 cgtactgggc cggcgtggcc gtgaacgcga tcgcgccggc gcgcggcggc gacgtcgtgc
     3901 gcctctacat cgcgaagcac cggctccagg ggacgacgta cccgacgatc atgtccacgc
     3961 tcgtcgtcga gacgctcttc gacttcgtgc tcgcaaccgc gctcttcctg gccgcggtgc
     4021 agatgggcct cctccccggg ctcggcgacc tgccctcgct cccggcgttc gactggtcgt
     4081 tcgcggtcga gcacccggcg atcgcggcgc tcctcggatc cgtgttcgtc gcgggcctca
     4141 tcctcttcgc gacgtgggcg tcgcggcacg tggtcgcgtt caaggagaag gtgaagctcg
     4201 ggttcgcgat cctcggcgac tggcggaagt tcgtcaccca ggtcgtctcg tggcaggcgc
     4261 tctcgtgggt cttccggatc ctcgccaccg cgttcttcat gaacgcgttc gggatcccgg
     4321 tgaacgtcga gacggtgctc gcggtcgtcg tcgtcggcgg gctcgcgacg acgatgccgt
     4381 tcacgccggg cggcgccggg acgcagcagg cggtgatcgt gttcgcgctc tccgggtggg
     4441 cgtcgtccag cgcgatcctc tcgttcagcg tcggcatgca ggtcgcgacg gccgcgctga
     4501 ccgtgtccgt cgggttcgcg gcgatcgcga tcatgctcgg caccgtccgc tggcgcgagc
     4561 acgtgcgccg cgacgaggac ctcatgcggc gaaaggggcc cgacggcccg gagacgccct
     4621 cggctaacgg ggagccagcg cccgcaccgt ctgggcgagc tcgtcgagcg tgaacggctt
     4681 cgcgaggaac ccggccgcga tctcgcccca ggcgtcgcgg cgctcgacgc tgcccgtgaa
     4741 gagcgcgacc ccgagctcgg ggcggctcgc gcggagctcc ttcgcgagct cgagcccgtc
     4801 gtcggcgccg aggtggacgt cgaggagcag gacgtcgacg tcctcgccgt cgagaacgcg
     4861 ggtcacctcc tccgccgtct cggcctcgag cacgcggtag ccgtccagct cgaggttcac
     4921 gcggcagagc agtcgcaggg ccgcgtcgtc gtccgcgacg agcaccgcac gctgttgcgc
     4981 gtcctccacg cgcgcgagtc tagatggagc gggatatcat cgcgccatgg agcgagcggt
     5041 tcggaccggc gagcggcgcg ggtacggggc ggcggtggag gggctcctcg agccgctcgg
     5101 cgggacgctc atccggctcg agcgcgtcgc cgaggcgacg ccggaggaga tcgtcgcgga
     5161 ggcgcacgag gagctcccgg gcctccagta cacgctgcac gcgagcgccg agctcgcgct
     5221 cggcctcgac ccgccggcgc gcgtcggccc gctgcacgcc gagctcgttg acgcgctcgg
     5281 acatgcccgc gacgcgaccg ccgaggtgtc ggctgcggtc gtggagggcg acgacgacct
     5341 cgtcggaaag ctcctgcccg agtggcgcgg cgcgctcttc cgcgtgcgcc tggctcgcct
     5401 gcgggccctc gagcgctcgc cgctccggct gccgcccgag gacgtggcgc gcgtccaccg
     5461 cgggcgcggc gaggtcaccc cgacggcgac gacgatcgtc gccaccggcc tggtcgtcgc
     5521 cggagcggtg ctcttcaccg ccggcgccgt gctcgcgtcc tggccgctct gggcggccgg
     5581 gctcgcgctc ttcggcggcg gcttcgtcct gttccggccg taaccggcgg cggcccctca
     5641 ccgttgagcc gctaccgggg gagcacgatg gaccgagacg cgatgacagc actgcggcag
     5701 gcgctcacgg cggggatcgc atggccgacc ccggccgagc ggcggcgtgc gtggcggtcg
     5761 ttcgagcggg cgctcgaccg gcacctggcg acacgaccgg agcggcggct caggccgatc
     5821 gggacgcggc gctgaggcgc gcgaggctct cctcgcgccc gaggagctcg agcgactcga
     5881 agaggccggg cgacacgttc gacccggtga cggcggcccg gatcggcccg aacgccttgc
     5941 gcggcttgag ctcgagccgc tccgcgagcc cgcggagggc ctgctcgatc tcgggcgccg
     6001 tgaacggctc gacctcggcg agcgtctgct cggcctccgt cagcagatcg gccgagccgt
     6061 cgacgagcgc cgggtccggc tcgacgcgcc cgaagaagaa gcccgcgaac gccgggaact
     6121 cgccgagggt cgcgatcttc tcctggcaga gcggcgcggc tgcgcggatc gtcgcctcgt
     6181 cgccctcgaa gccctgctcg cgcaggtagc cgacgagccg gtcggcgtac tcctcgggcg
     6241 ggaggtcgcg gaggtagacg ccgttcagcc actcgagctt ctggtagtcg aacgtcgccc
     6301 cgctcgcgcc gacgcggtcg agcgtgaagt ggcgcacgag ctcctcgggc gggatcacgg
     6361 tcgtctcggc gtcgaggctc cagccgagga gcgcgaggta gttgacgatc gcctcgggca
     6421 ggtagccgcg ctcccggaac tcctcgacgg acacggcccc gtggcgtttc gagagcttcc
     6481 gcccgtcagc gccgaggacg ttgggcaggt gcgcgtacgt cgggagcggc gcgccgagcg
     6541 cctcgaggat ccggatctgc ttcggcgtgt tcgcgatgtg atcctcgccg cgtacgacgt
     6601 gcgtgatccc gtcgtccatg tcgtccacgg gcgaggcgaa gttgtaggtc ggccgcccgt
     6661 ccgagcggag cagcacgacg tcctcgagct tctcgttcgg gaactccacg cggccgcgaa
     6721 cgacgtcctc ccagccggtc ttgccctcgt ccggcatgcg gaagcgaatc gcgccctcgt
     6781 cctcgtacgc gtggccggcc tcgaggagcc gccgcgcgac ctcctggtgg cgctcgagcc
     6841 ggtcgagctg gaacgcgacc tcgccgtccc agtcgagccc gagccagcgg agcgactcct
     6901 ggatctgctc gacggactcc tccacctcgc ggctgcggtc ggtgttctcg acgcggagga
     6961 ggaactcgcc gccctcgtgg cgcgcgaaga gccagttgaa gagcgcggtg cggacgccgc
     7021 cgatgtggag gaagccggtc gggctcgggg cgaagcggac tcggggggcc acggcgccac
     7081 tagggtaacg gcgtgctgct cggcggccac atgtcttcgt ccggcgggat ccacaccgcg
     7141 gtcgaccgaa tcgaggcgat cggcggcgac gcgctccagg tcttcacgca gagcccgcgc
     7201 atgtggcggc cgaccgcgca cccggaggag aacctcgcgc ggttccgttc ccggcgggag
     7261 gaggccggca tcgggtcggt gctcgcgcac gcggtctacc tcatcaacct cgcctcgccc
     7321 gacgacgagc tctacgagaa gtccctcacc gcgctcgtct ccacgatgga ggtcgcgtcg
     7381 gcgatcgagg cggacggcgt cgtcttccac gtggggtcgc acctcggagc cgggttcgag
     7441 gcggggatgg agcgagcggc gccggcgatc gagcgggcgc tcgagcatgc gtccgagaag
     7501 acctggctcc tgctcgagaa ctgcgccgga accggcggga cgatcggacg atcggtcgac
     7561 gagctcgcgg cgctgtgcga ccggctcggc gggcacgaac ggctcgggct ctgcctcgac
     7621 tcatgccacc tgtgggcgtc cggcgtggac gtgacggatt cggccgtcct ggacgcggcg
     7681 ctcgacgaga tcgacgagcg gatcgggctc gaccggctgc gggcgctcca cgtgaacgac
     7741 tcgcgcgaca cgctcggggc gaaccgcgac cggcacgcga acatcggcga gggcgagctc
     7801 ggcgagcggc tcgcggtctt cctcggcaac ccgcggctcc aggggctgcc cgcggtgatg
     7861 gaggttccgg gcccggacgg gcacgggccg gacgccgggg agatgcggaa gctgcgggag
     7921 ctccacgcgc gcgcggtgaa ggcgtacgcc gcggcctgac tcgtctgacc ggcctgtccg
     7981 gctcggccgc gtgtggagcg atcggcacgg tcacggcgca caatcggcac gatccgtgcg
     8041 gattcccagg ggtacgctcg tcgcgggaag gggtggtcac ccccacccag ggcggggccg
     8101 cgcgcgcgtg taactcaggg tggaagagcg aggcgccgtg agcagctccg gagcccggaa
     8161 gcgaggacgc cgcccggtca cggctctgca cacgacgaag cgctagcgcg agcgcggctt
     8221 gcgcccgcgg gcgagggcgc gctgcgtgtc cggcccggcc acgccgtcga cctcgagccc
     8281 ggtgcgcgtc tggaaatccc gcacgaccgc ctcgacgcgc acgccgtagc tcctccccgg
     8341 ctcgatccgg aaggcggccc aggcgtcggg tgcatgcttc gagtaccacg cctcgagccg
     8401 cgccttcagc tcctcgacgc gcttgccgcg cgagccgcgg cggaggagcg gcggcggcgg
     8461 ctcgacgccg acgagctcct ggtacacggc ccagcgcgcg agcaccgagc ggccgtagtc
     8521 gccgcccgtc gtccgcttgt cggagtcgcc ctcgcgatac ccggcgagcg ccccgccccc
     8581 gcccgcgttg tacgccgaga gcgcaaagag cagccggtcg cgctcgggga cgccgctgcg
     8641 cgcgccgaag tcccagttcc agcggaggag gtcggcggcg tagcgggcag cggccggcac
     8701 gggcggcttc ccgccggcgc gcgcggcgcc acttcgtgcg aggaagtcgc ggtgcgaccg
     8761 gtcgtcgatc tggaagaggc cgcgcccgtg cccgccgtcg ccgaccacgt cgttcatgtc
     8821 ggtctcctgg gacgcgacgg cgaggaggag cgccgccggc aggcgggccg ccttctccgc
     8881 gtccgcgcag gccttgtccc acccggcgcc ccgcgcgcgg tcgagctcct tcgcgagcgc
     8941 cgtcttcgtg aacgcgcgca ccgcgggcgc cggacgtcag tcggcgtcga tccgcgcgat
     9001 cacgcggaag gacgggcttc cctgcgggag ctcgaagaac tcggggttca cgcagccgag
     9061 gcggtcctgg cggtgctcgt agcacgagga gacctcggcg cggcacatcg gaagcggctg
     9121 ccggcaggcc ttgaccgcgc cgaagcccgg cttcgccccc tgggcctcct ccagcagctc
     9181 gagatcgatc tcgaccgcgt agaccttctg catgcagccg acgtacgtcc tcccgagctc
     9241 ctcgtacgcg tagaggaacg ggcacgcgcc ctcgacgcaa gcggcggggt acacgacctt
     9301 gtcgcaatgg acgtcgcagc gccgacactc cgtctcgtcc tggggacgga ggggtaggtc
     9361 ggagatgacc ttgaggggcc gttccactgc cttcgattgt gggcgaacca tccggccccc
     9421 gcaagacttg acagcgccgc cgagcccgtg taaaccgccg cgcttgcagg gcttccgttg
     9481 cctgcgccga cggcgctagg cctcgccgcg cagccgccgc accgcgttca ggacgtccat
     9541 cggcgtgaaa gcctcgaagt ccggctccgg ctccccgtcg tcggggtcgg cccggaacca
     9601 gagagcctgc gccgtccgca tcccgagcgc ctgcgcaccg cggatgtcgg cgttccggct
     9661 gtccccgacg aagagcgcgc gctcgggcgc cacctcgagc gcctcgaggg cgcgccggaa
     9721 gatctccgga tgcggcttcc gcaggccgac ctccgaggag aagacggccg tgtcgagccg
     9781 ctcggcgagc cccatccgct cgaggtcctc gtggaggagc cacgccgggt cgaacgcgtt
     9841 cgagacgagc ccgagcttca gcccccgcgc gcgcagcgag tcgaggagcg cgtgcgtctg
     9901 cgcgcccatg acgcgcgccg gctgccaggt ggcgtgctcg gcctcgagga accggccgag
     9961 ctcctcctcg tcgaccgaga cgccgaagcc gtcgagcacc tcgcggatca cgccggggta
    10021 ctcgacctcc tcgagcgtct cggtgccgac gaggagcggc aggtagcgct cccggaaccg
    10081 tgcggccgtc tcgtcgtggc cgggcagccc ctcgcgcccg atcgcggcga gcccggcctc
    10141 ccagccgcgc tcgagcagct cgtcgtcgta ggcgaagtgg aagagcgtat cgccccagtc
    10201 gaagaggacg gcgtcgatca cccggggagc gtaggcgacg gacgcgggct cgccgttcgg
    10261 ttagccgcac gttaagagga gggaacgccg ccttgatcga gcggagaccg gttgaaaggg
    10321 tgtgcggacg cccgactcgt caggaggttc tctcgtgcag caggcatccg tctcacccga
    10381 atccccgccg gtgcaggccg acgacggcct cgccgcgtcc gcgcacgacg tcacgcgcgt
    10441 ctacggcgag ggcgacacgg ccgtcgaagc gctgcgcggc gtgtcgctcg acgtcggccg
    10501 cggggagctc atctcgatca tgggcccctc gggctccggc aagtcgacgc tcatgcacgt
    10561 gatggccggg ctcgacaagc cgacctccgg cgacgtcgcg atcgccggca cgaacatcgc
    10621 cgcgctgaac gacacggagc tgacgaagct ccggcgcgcg cacatcggct tcatcttcca
    10681 gttcttcaac ctgcttccga tgctgaccgc ggaggagaac atcaccctcc cgctccagct
    10741 cgccggcggg aagcaggacc gcgactgggt cgagaacgtg atcgacaagg tcggtctcgc
    10801 cgaccgccgc acgcaccgcc cgtcggagct ctccggcggc cagcagcagc gcgtcgcgat
    10861 cgcccgcgcg ctcgtctcgc gcccgacggt cgtcttcgcc gacgagccga ccggcaacct
    10921 cgactcgaag acgggcgggg agatcctcga ggtcctccgc gagtccgtgc gcgactacgg
    10981 ccagacgacc gtgatggtca cgcacgacgc gaacgccgcc tcgatcggcg accggacgct
    11041 cttcctcgcc gacgggctcg tgatgaagga gctccgcgcc tcgtcgccgc acgacctgct
    11101 gaccgcgatg gaggaggtca gctcctgatg gtgcgggtcg cgctcaaggg actcgcgggc
    11161 cgcaagctcc gcgccgcgct gacggcgatc gcgatcgtcc tcggcgtcgc gatggtgagc
    11221 ggcacgtacg tcctcaccga cacgatcgac aaggcgttca gcgcgatctt cgaggagtcg
    11281 tacgcgaaca ccgacgcggt cgtccgcggc gccggctccg acatctcgtt ccaggggatg
    11341 tccggcccgt ccgcgccgat cgaggacgac ctcctcgacg acgtcctcgc cctcgacagc
    11401 gtcgagaccg ccgcgggcgg catcgaggac gagatgaaca cgaagatcat caagaaggac
    11461 ggcacggccg tgaacacgca gggctcgccg tcgctcggct tcggcgtcga ccccgaggcg
    11521 agcgagttca acccgctcac cctcgtcgag ggcgcgtggg c
//
LOCUS       DASTFC010000380         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1251322,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000380 DASTFC010000000
VERSION     DASTFC010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251322"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..289
                     /locus_tag="VFO88_11175"
     CDS             83..289
                     /locus_tag="VFO88_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET9546127.1"
                     /translation="MATGTVKWFSNDKGYGFITPDDGSKDLFVHFSNIASEGFKSLQE
                     GERVQFEAREGAKGPEATDVATIA"
     gene            364..582
                     /gene="infA"
                     /locus_tag="VFO88_11180"
     CDS             364..582
                     /gene="infA"
                     /locus_tag="VFO88_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9546128.1"
                     /translation="MSQEDKIEMEGEVVEALRNRMFRVQLDNGHETLGYTAGKMRRYR
                     IRIFPGDRVKIEISPYDLNRGRIVYRYR"
     gene            611..1855
                     /locus_tag="VFO88_11185"
     CDS             611..1855
                     /locus_tag="VFO88_11185"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HET9546129.1"
                     /translation="MAVSSGVKLDARALRADFPILARPMGGKPLAYLDSSNSSQKPRQ
                     VMDAEREYYETSYANVHRALYELGEKATAGYEGARETVRAYLNAETTREIVFTRGATE
                     ALNLVAYAWGLDNLGPGDLVVSTELEHHSNFVPWQFIAGRTGAEFAVVPANEHGELEL
                     EALDELAARGTVKVVAVGYVNNSLGTVNPVERIAAWAREQGAITVVDACQAAPHRRID
                     VQALGADFVAFTGHKMCAPTGIGVLWGRRELFEAMSPFGLGGDMIRSVSVEKTTWNEL
                     PQKFEAGTPPFAQAVGLAAAIDYLEAAGLEAIEAHEADLLEYTLGRLGELPQVKVWGP
                     PAERRGGIVSFDVAGVHPHDVAQILAYDGVAVRAGHHCTQPLMTRLGVPATTRASFYL
                     YTLPEEIDRFVEGLHRVTKTFA"
     gene            1848..2465
                     /locus_tag="VFO88_11190"
     CDS             1848..2465
                     /locus_tag="VFO88_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546130.1"
                     /translation="MPDLVAVDETEFGFGWVDETGSVPRTSHALAVDGRVWVIDPVLV
                     DGLEERLRALGEPAGVLVLLDRHQRDSRAVAALLGVPVHETPFDGVDGAPFEFRRILR
                     TPVWREVALWWPERRVLVAADALGTLGYMTAGGEALGIHPMLRPWPPRQALAGLAPGH
                     VLVGHGAGVHGAEAARALADALANARGGIATLVADGFRAASRRLR"
BASE COUNT          367 a          893 c          923 g          365 t
ORIGIN      
        1 ggtacatcaa gagcgcggtg ttcggtttgc ggctttgccc ttccttaccc ggttcgtgag
       61 tgtgtgtgag ggaggtgttt caatggctac cggaaccgtg aagtggttct cgaacgacaa
      121 ggggtacggc ttcatcaccc cggatgacgg gtccaaggac ctgttcgttc acttcagcaa
      181 catcgcctcc gaaggcttca agagcctgca ggagggggag cgcgtccagt tcgaggcgcg
      241 cgagggtgcc aagggccccg aggcgacgga cgtcgccacg atcgcctaga ccgcttgccg
      301 accggggccg ctcgctcggg atccgcgcgg gcggcctcgt cgtattcgaa gggaggaagc
      361 ggcatgtcgc aagaagacaa gatcgagatg gagggcgagg tcgtcgaggc gctccggaac
      421 cggatgttcc gcgtgcagct cgacaacggc cacgagaccc tcggctacac cgccggcaag
      481 atgcgccggt accggatccg gatcttcccg ggcgaccgcg tgaagatcga gatctccccg
      541 tacgacctga accgcggccg catcgtctac cgctaccggt agggccgccc cagggcggta
      601 cgatggccgt atggccgtct cgtccggcgt gaagctcgac gcgcgtgcgc tgcgcgccga
      661 cttccccatc ctcgcgcggc cgatgggcgg gaagccgctc gcgtacctcg acagctcgaa
      721 ctcgtcgcag aagccgcgcc aggtcatgga cgcggagcgc gagtactacg agacgtcgta
      781 cgcgaacgtc caccgcgcgc tctacgagct cggcgagaag gcgaccgccg gctacgaggg
      841 cgcgcgggag accgtccgcg cgtacctgaa cgccgagacg acccgcgaga tcgtcttcac
      901 gcggggcgcg accgaggcgt tgaacctcgt cgcgtacgcg tgggggctcg acaacctcgg
      961 gcccggcgac ctcgtcgtct cgaccgagct cgagcaccac tcgaacttcg tcccctggca
     1021 gttcatcgcg ggccgcacgg gcgcggagtt cgccgtcgtc ccggcgaacg agcacgggga
     1081 gctcgagctc gaggcgctcg acgagctggc cgcgcgcggg accgtgaagg tcgtggcggt
     1141 cgggtacgtc aacaactcgc tcggcaccgt gaacccggtc gagcggatcg ccgcctgggc
     1201 gcgcgagcag ggggcgatca cggtcgtgga cgcctgccag gcggcgccgc accggcggat
     1261 cgacgtgcag gcgctcggag cggacttcgt cgcgttcacc gggcacaaga tgtgcgcgcc
     1321 gaccggcatc ggggtcctct ggggccgccg cgagctcttc gaggcgatgt cccccttcgg
     1381 gctcggcggc gacatgatcc gctcggtctc ggtcgagaag acgacctgga acgagctgcc
     1441 gcagaagttc gaggccggga cgccgccgtt cgcgcaggcg gtcgggctcg cggccgcgat
     1501 cgactacctc gaggcggccg ggctcgaggc gatcgaggcg cacgaggccg acctgctcga
     1561 gtacacgctc gggcggctcg gcgagctccc gcaggtgaag gtctgggggc cgcccgccga
     1621 gcgccgcggc gggatcgtct cgttcgacgt cgccggcgtc cacccgcacg acgtcgcgca
     1681 gatcctcgcc tacgacgggg tcgcggtgcg cgcggggcac cactgcacgc agccgctcat
     1741 gacgcggctc ggcgtccccg cgacgacccg cgctagcttc tacctctaca cgctcccgga
     1801 ggagatcgac cgcttcgtcg agggcctcca tcgcgtcacg aagacgtttg cctgacctcg
     1861 tcgcggtcga cgagaccgag ttcggcttcg ggtgggtcga cgagacgggc tccgtgcccc
     1921 gcacctcgca tgcgctcgcc gtggacggcc gcgtctgggt gatcgacccg gtgctcgtcg
     1981 acggcctcga ggagcggctc cgcgcgctcg gggagccggc cggcgtgctc gtcctcctcg
     2041 accggcacca acgcgattcc cgcgccgtcg cggcgttgct cggcgtgccc gtgcacgaga
     2101 cgccgttcga cggcgtcgac ggtgccccgt tcgagttccg ccgcatcctg cggaccccgg
     2161 tctggcgcga ggtcgcgctc tggtggccgg agcggcgcgt cctcgtcgcc gcggacgcgc
     2221 tcgggacgct cggctacatg accgccggcg gcgaggcgct cgggatccac ccgatgctcc
     2281 ggccctggcc gccgcggcaa gcgctcgccg ggctcgctcc ggggcacgtc ctcgtcggcc
     2341 acggcgccgg cgtgcacggc gcggaggccg cgcgcgccct cgccgacgcg ctcgcgaacg
     2401 cgcgcggcgg catcgcgacg ctcgtggcgg acgggttccg ggctgcatcc aggcggctac
     2461 gatgaccgtg tgaacgagtt cgacgccatg taccgcgagg tcatcctcga ccactacaag
     2521 aacccgcgcg ggcacgggga gatcgagg
//
LOCUS       DASTFC010000381         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_789766,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000381 DASTFC010000000
VERSION     DASTFC010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789766"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VFO88_11195"
     CDS             <1..1492
                     /locus_tag="VFO88_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9546131.1"
                     /translation="IVVIAHRDNSGISPGVNDNASGTGALLELARTVETTLPSHTLVF
                     LSTDGGAFGGIGAARFAERPQLLRRILGSTGTPVAVVNLDSIGSGAEPRLLFAGDAAR
                     SAPATLLATAGASIERETGASPQRPSGLAQLVDLGFPFALHEQGPFVARGIPAVTITT
                     GSERPDTQGPDTSEELRRDQLGSLGRAAQDLLLRLDGSPELASATQSYLYVGERLVRG
                     WAIQLFLLAALLPFLAATLDLFARCRRRHVALLPALRSFGRRLGVWLWIGLVFLLFSL
                     AGFFPGGDARPLNPNADVVGEWPVVALAAVALLGGGAWVLAHGRLARRGGVAREDELG
                     GYLAVFLVLALVALVVAATNPYALVFLLPTLHAWLWLPNVPRENGLLRAAVFAAGFLG
                     PLLLFASFALRLGLGLDAAWYVLALVSVGYVTPPLVLAVLVWAATAEQVGALAAGRYA
                     PYPPPDERTGGPLRDAGRALAGLVRSRRAPAVEAEQEQPRLRVVED"
     gene            complement(1489..2694)
                     /gene="glp"
                     /locus_tag="VFO88_11200"
     CDS             complement(1489..2694)
                     /gene="glp"
                     /locus_tag="VFO88_11200"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET9546132.1"
                     /translation="MAELLSLADAQRLVLEHARPLEAERVPLERAAGRVLAEPVAAGV
                     DLPPFPSSAMDGFAVRAADTYGAPVTLPLAGEAAAGSPLAGPLPAGHVARISTGGVVP
                     DDADAVVPLEVVEETDAGILFRAPARPGDHVRERAGDVRAGETVLEPGAALGPAQVAA
                     LAAAGVSEVRCTKRPRAAILVTGSELRPPGAELAPGEIYESNGLLLAAALGSAGAVPA
                     QLGVVPDEGAELERALERALLGFDLVVTSGGASVGPHDLVREALRGLRVEEVFWGVAM
                     KPGKPVAFGVRRDHLVFSLPGNPVSALVTFELLVRPAVNALHGLADPFPAFGHGTLAA
                     PIARNAHRLEFVRARLRTGEDGTPVLEPLAGQESHMIARAAPASALVEVEPGEGELAD
                     GSTVRFLRL"
BASE COUNT          304 a         1110 c         1022 g          358 t
ORIGIN      
        1 gatcgtcgtc atcgcccacc gcgacaactc cgggatctcg cccggcgtga acgacaacgc
       61 ctccggaacc ggagcgctcc tcgagctcgc gcgcacggtc gagaccaccc tcccgagcca
      121 cacgctcgtc ttcctctcga cggacggcgg cgccttcgga ggcatcggcg cggcgcgctt
      181 cgccgagcgg ccgcagctcc tgcgccggat cctcggctcg acggggacgc cggtcgccgt
      241 cgtgaacctc gattcgatcg gaagcggggc ggagccgcgc ctcctcttcg cgggcgacgc
      301 ggctcgctcg gccccggcca cgctcctcgc gacggcgggc gcgagcatcg agcgcgagac
      361 gggcgcctcc ccgcagcggc cgtccggcct cgcgcagctc gtcgacctcg gcttcccctt
      421 cgcgctccac gagcaggggc cgttcgtcgc ccgcggcatc ccggcggtca cgatcacgac
      481 cggcagcgag cggcccgata cgcaggggcc cgacacctcc gaggagctcc ggcgcgacca
      541 gctcggctcg ctcgggcgcg cggcgcagga tctcctcctc cggctcgacg gctcgcccga
      601 gctcgcgagc gcgacccagt cctacctcta cgtcggggag cggctcgtgc gcggctgggc
      661 gatccagctc ttcctcctcg ccgcgctcct gccgttcctc gccgcgacac tcgacctctt
      721 cgcccgctgc cgccgccgtc acgtcgcgct cctgcccgcc ctgcggagct tcggacggcg
      781 cctcggcgtc tggctctgga tcggcctcgt cttcctcctc ttctcgctcg cggggttctt
      841 ccccggcggg gacgcgcggc cgctgaaccc gaacgcggac gtcgtcggcg agtggcccgt
      901 cgtggcgctc gcggcggtcg ccctcctcgg cgggggagcg tgggtgctcg cgcacgggcg
      961 cctcgcgcgg cgcggcggcg tcgcgcgcga ggacgagctc ggcggctacc tcgccgtgtt
     1021 cctcgtgctc gcgctcgtcg cgctcgtcgt cgcggccacg aacccctatg cgctcgtctt
     1081 cctcctcccg acgctgcacg cgtggctctg gctcccgaac gttccacgtg aaaacgggct
     1141 cctgcgcgcc gccgtcttcg cggccggctt cctcgggccg cttctcctgt tcgcgtcgtt
     1201 cgcgctgcgg ctcgggctcg gactggatgc cgcgtggtac gtcctcgcgc tcgtctccgt
     1261 gggctacgtc acgccgccgc tcgtgctcgc cgtgctcgtc tgggcggcga ccgcggagca
     1321 ggtcggcgcg ctcgccgccg gccgctatgc gccctacccg ccgccggacg agcgcacggg
     1381 cggcccgctc cgcgacgcgg ggcgcgcgct ggccgggctc gttcgctcgc gccgcgcgcc
     1441 ggccgtggag gccgagcagg agcagccgcg gctccgcgtc gtggaggact agaggcggag
     1501 gaagcggacg gtcgagccgt ccgcgagctc gccctcgccc ggctccacct cgacgagcgc
     1561 ggacgcaggg gcggcgcggg cgatcatgtg cgactcctgg cctgcgagcg gctcgagcac
     1621 gggcgttccg tcctctcccg tgcgcaggcg ggcgcgcaca aactcgaggc ggtgcgcgtt
     1681 ccgcgcgatc ggggccgcga gcgtcccgtg cccgaaggcg ggaaacgggt cggcgagccc
     1741 gtggagggcg ttcacggccg gacgcacgag cagctcgaac gtgacgagcg cggacacggg
     1801 gttccccggc agcgagaaca cgagatggtc ccgccggacg ccgaacgcga cgggcttccc
     1861 cggcttcatc gcgacgcccc agaagacctc ctcgacgcgc aggccgcgaa gcgcctcgcg
     1921 gacgagatcg tgcggcccga ccgaggcgcc gcccgacgtg accacgaggt cgaagccgag
     1981 cagcgcccgc tcgagcgcac gctcgagctc cgcgccctcg tccggcacga cgccgagctg
     2041 ggcggggacg gcgcccgcgc ttccgagggc cgcggcgagc aggagcccgt tcgactcgta
     2101 gatctcgccc ggcgcgagct ccgcgcccgg cggccggagc tccgacccgg tgacgaggat
     2161 cgccgcgcgc ggccgcttcg tgcaccgcac ctccgagacg ccggccgcgg cgagcgccgc
     2221 aacctgcgcg gggccgagag ccgccccggg ctcgagcacc gtctcgccgg cgcgcacgtc
     2281 gcccgcgcgc tcgcggacgt ggtcgcccgg acgcgccggc gcgcggaaga ggatccccgc
     2341 gtccgtctcc tccaccacct cgagcgggac gacggcgtcg gcgtcatccg ggacgactcc
     2401 gccggtcgag atccgcgcca cgtgacccgc tgggagcggc ccggcgagcg ggctccccgc
     2461 cgccgcctcg ccggcgagcg ggagcgtgac cggtgcgccg taggtgtccg ccgcccggac
     2521 ggcgaacccg tccatcgccg agctcgggaa cggcggcagg tcgacgccgg ccgcgacggg
     2581 ctcggcgagc acgcgacccg ccgcacgctc gaggggaacc cgctcggcct cgagcggtcg
     2641 cgcgtgctcg aggacgagcc gttgcgcgtc ggcgagggag aggagctcgg ccacggccga
     2701 tacgctaacc ggttgcccgc tcgggcaacc ggttgctccg cctacccggt tatcgtcggc
     2761 ggcgggggcg gcggctcggt ggtcgtcggc ggcg
//
LOCUS       DASTFC010000382         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_16078,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000382 DASTFC010000000
VERSION     DASTFC010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16078"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFO88_11205"
     CDS             complement(<1..795)
                     /locus_tag="VFO88_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9546133.1"
                     /translation="MPVKRLLMTPGPTPVPPEVFAAMSQPIVHHRTKDFRLLLDRLLD
                     RLRAVHRTENDLLLFAASGTGGMDGAVANLCSPGERVLCVSAGNFGERWVQLAEAYGC
                     DVVPVRYEWGETPQPDDVAAALEEAGGARAVFLTHSETSTGVVADVDAIARRLRGSGV
                     LTVVDAISSLGAVPLSVDESGIDVVVSGSQKALMTPPGLATTSVSAAALAAALESRSP
                     RFYFDWARNLKALQGDPPATVFTSAVSLVYGLDAALGLILEEGLDAV"
     gene            835..1536
                     /locus_tag="VFO88_11210"
     CDS             835..1536
                     /locus_tag="VFO88_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546134.1"
                     /translation="MEALLAFAAALLAFRFAGELAARWRDRRRPELLAWSGSLLAYAL
                     ASGALAWGAAAGWNEASFRLYYLFGGLLTAALLGAGSLLFAGRRWAGPLALVYTGLAV
                     GLVLAEPLTAPIGGTTIPEAQAHLDVFPVRIVAILGNSLGTIAVVAVALLTLRARPLG
                     NALVVAGTSVAAAGTAVAGLGAAPTAAFVALAVVLLYAGFLGLSGLPLRRPASGGSAT
                     STRPASYPTHSSSRP"
     gene            complement(1469..>2168)
                     /locus_tag="VFO88_11215"
     CDS             complement(1469..>2168)
                     /locus_tag="VFO88_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546135.1"
                     /translation="GDPPVPANSCRIYANNLDTQNYQHCITANFTGTDKHGECVEATP
                     APVGDPILVVKQGSGAGTVTSTPAGISCGSTCSAVFPLDPANPVSLFAEAAPGSTFQG
                     WSGQGHSCTTGACNVTVQGQTIRVVATFSGTPPPPPPPAILNAVILQKPAKTTRKRSA
                     RFVWAAKRGTSFVTTFKSQCRLNKQVWKACAPPKTYRNLRAGRSHTFRVRVKDAKRNE
                     WDKTPAVWTWRIRR"
BASE COUNT          262 a          783 c          805 g          318 t
ORIGIN      
        1 gacggcgtcg aggccctcct cgaggatgag gccgagggcc gcgtcgagcc cgtagacgag
       61 cgagacggcc gaggtgaaga cggtcgcggg cgggtcgccc tggagcgcct tcaggttccg
      121 ggcccagtcg aagtagaagc gcggcgagcg gctctccagc gcggcggcga gcgcggcggc
      181 cgacacggag gtcgtcgcga ggcccggcgg cgtcatgagc gccttctgcg agccggagac
      241 gaccacgtcg atcccggact catccaccga gagcggcacc gcgccgaggc tcgagatcgc
      301 gtccacgacc gtgagcacgc cggagccgcg gagccggcgc gcgatcgcgt ccacgtcggc
      361 gacgacgccg gtcgaggtct ccgagtgcgt gaggaagacg gcgcgcgcac cgcccgcctc
      421 ctcgagcgcg gccgcgacgt cgtccggctg gggcgtctcg ccccactcgt agcgcacggg
      481 caccacgtcg cagccgtacg cctccgcgag ctgcacccag cgctcgccga agttccccgc
      541 cgagacgcag aggacgcgct cgccgggcga gcagaggttc gcaacggcgc cgtccatccc
      601 gccggtcccc gaggcggcga agaggagcag gtcgttctcc gtccggtgca ccgctcggag
      661 ccggtcgagc agccggtcga gcaggagccg gaagtccttc gtccggtggt ggacgatcgg
      721 ctgcgacatc gcggcgaaga cctccggcgg gacgggggtc ggcccgggcg tcatcagcag
      781 gcgcttcacc ggcatcggca ggactgaggt taccggccgc cgtacgattg acccgtggag
      841 gctctgctcg cgttcgccgc cgcgctcctc gccttccgct tcgcggggga gctcgccgcg
      901 cgctggcgcg accgccgccg gccggagctg ctcgcctggt cgggctcgct cctcgcctac
      961 gcgctcgcct cgggcgcgct cgcctggggc gccgcggcag gctggaacga ggcgtcgttc
     1021 cggctctact acctcttcgg cgggctcctc accgcggcgc tcctcggcgc cgggtcgctc
     1081 ctcttcgccg ggcgccgctg ggccgggccg ctcgcgctcg tgtacaccgg gctcgcggtc
     1141 gggctcgtcc tcgcggagcc gctcaccgcg ccgatcggcg gaacgacgat cccggaggcg
     1201 caggcgcacc tcgacgtctt tcccgtccgg atcgtcgcca tcctcgggaa cagcctcggg
     1261 acgatcgcgg tcgtcgcggt cgcgctcctg acgctccgcg cgcgaccgct cgggaacgcg
     1321 ctcgtcgtcg cggggacgtc cgtcgcggcg gcgggcaccg cggtcgcggg cctcggcgcg
     1381 gcgccgacgg cggcgttcgt cgccctcgcg gtcgtcctgc tctacgcggg gttcctcggg
     1441 ctctcggggc tgccgctgcg ccggcccgct agcggcggat ccgccacgtc cacacggccg
     1501 gcgtcttatc ccactcattc ctcttcgcgt ccttgacgcg aacgcggaac gtgtgcgagc
     1561 gcccggcgcg caggttgcgg taggtcttcg gcggcgcgca cgccttccac acctgcttgt
     1621 tcagcctgca ctgcgacttg aacgtggtga cgaaggacgt gccgcgcttc gcggcccaga
     1681 cgaaccgggc ggagcgcttc cgcgtggtct tcgcgggctt ctggaggatg acggcgttca
     1741 ggatcgcggg cggcggcggg ggcggcggcg ttccggagaa cgttgcgacg acccggatgg
     1801 tctggccctg gacggtgacg ttgcaggcgc cggtggtgca ggaatggccc tgaccgctcc
     1861 atccctggaa tgtggacccg ggcgcagcct cagcgaacag cgacacgggg ttggcgggat
     1921 cgagtgggaa cactgcagag cacgtcgacc cgcagctgat cccggccgga gtgctcgtca
     1981 ccgtgcccgc gcccgatccc tgcttcacga cgagaatcgg gtcgccaacc ggcgccggcg
     2041 tcgcctcgac gcactcgccg tgtttgtcgg tacccgtgaa gttcgcggtg atgcaatgct
     2101 ggtagttctg ggtgtcgagg ttgttcgcgt agatccggca gctgttcgcc gggaccggcg
     2161 ggtcgccc
//
LOCUS       DASTFC010000383         7562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_477831,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000383 DASTFC010000000
VERSION     DASTFC010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477831"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..526
                     /gene="rplK"
                     /locus_tag="VFO88_11220"
     CDS             101..526
                     /gene="rplK"
                     /locus_tag="VFO88_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET9546136.1"
                     /translation="MAKKVLTLIKLQIPGGQANPAPPVGPALGQHGVNIMEFCKAFNA
                     ETAQAGGRITPVEITVFEDRSFTFITKTPPAAVLIKEALNIEKGSGEPNREKVGRLTR
                     DQVRQIAETKLVDLNARDVDEASKVIAGTARSMGVEVDL"
     gene            523..1260
                     /gene="rplA"
                     /locus_tag="VFO88_11225"
     CDS             523..1260
                     /gene="rplA"
                     /locus_tag="VFO88_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003637773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET9546137.1"
                     /translation="MSAHGKRYRDARAAIDRERLYSPLEAIRLLKGAGETKFDETVEV
                     HFNTGLNVRHAEQQLRGTLMLPHGTGSDVRVAVFAEGEKAKEAQDAGADVVGAADLAT
                     QIEEGFDDFDLTIATPDMMGTVGKLGRILGPRGKMPNPKAGTVTFDVGKAVSDAKAGK
                     LEYRTDRGANVHLSMGKRSFDERALLENYAAILDEIMRAKPAAAKGRYIRSVTITSSM
                     GPGIKIDPARTRDLLAELEEEPQPAVA"
     gene            1390..2076
                     /gene="rplJ"
                     /locus_tag="VFO88_11230"
     CDS             1390..2076
                     /gene="rplJ"
                     /locus_tag="VFO88_11230"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET9546138.1"
                     /translation="MLKQEKERVVAELAERLQASETLLVADYRGLDMVEIDAVRTELI
                     KHGARFTVVKNTLTKRAAEQAGVPELNEFLDGPTAIAFVGDGDMVAVAKALNDSAKQT
                     KILALKGGILAGKPISADDVRELAALPPMEQLRGQVLGAIVAPLNAIAGLVNAPLQNL
                     LGLVDARIEQLREQGDAGEAAAEPQAEEPAAEEPAPDEEPAAEEAPAAEASEETETES
                     SEETNENEEE"
     gene            2076..2468
                     /gene="rplL"
                     /locus_tag="VFO88_11235"
     CDS             2076..2468
                     /gene="rplL"
                     /locus_tag="VFO88_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET9546139.1"
                     /translation="MAASGVDKVFDELGKMSVLDLVELKNKIEEEWGITAAAPVAVAA
                     PGAAAPGAADGGAAEEKSEFDVVLTAAGDKKIQVIKVVRAITGLGLKEAKDLVDGAPN
                     PIKEGVPQDEADSIKSQVEEAGGSVEVK"
     gene            2614..3399
                     /locus_tag="VFO88_11240"
     CDS             2614..3399
                     /locus_tag="VFO88_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546140.1"
                     /translation="MAARATIRSLARLGWTAALVGLAVLVVAAPASAKGPPSGKGDPP
                     PWAGGSGGKPSGGPPPWAGGSGKSAAAEQAKGAAKKAEKGLRKAEKKAAKAREGAEQN
                     PAFTCFALIGELGEEFYETYGTNPNKANAFGKCVSEHAREQGDGDEEEAGDEPAECEE
                     PAESEEPAEGDGDGEGDEPAGEEDPLAELASLDEPEPEPSEGECDPAGEDTGEEGEET
                     EGAEAGEPETEGETEGAAEAETGLRCFLPLAARANPFAVCART"
     gene            3534..4121
                     /locus_tag="VFO88_11245"
     CDS             3534..4121
                     /locus_tag="VFO88_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546141.1"
                     /translation="MNRKLTAALFVVAALSVVAAPQALAGGGKKPAKNAAKATCKAER
                     AALGTRLFRATYGATPVATCRRRVLDEAQEAVGNAAQQCFAERAADADAFREKYGQNA
                     NKRNAFGRCVSTLAKKELAEEVLETLNAAKECKAERGEDPDAFREKYGTNANKSNAFG
                     KCVSGKKAAEPGEEAPGEGEGEGEGDPGEGGGAEV"
     gene            4366..>7562
                     /locus_tag="VFO88_11250"
     CDS             4366..>7562
                     /locus_tag="VFO88_11250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9546142.1"
                     /translation="MATKVAPRPARKSFSRLKHVLDLPNLIDIQRASFDWFMKEGLRE
                     TIDDISPIEDYTGTLAVEFADYWFEDPPISIKECREKDLTYQAPLHMTVRFVNTETGE
                     IREQNVFMGDFPMMTESGTFIINGTERVIVTQLVRSPGAYLMEPKDPDKQVFIANLMP
                     SRGSWLEVEIDKKGVVYARIDRKRKLPITTLLRALPAEDPTTGFELDTSSNEKILELF
                     DNSPFIQLTLDKDTTTREEEAVVEVFKKQRPGEPPTLENARNLLRGLFFDPKRYDLTK
                     VGRYKLNQRLEVDVPDDVRVLTTQDIVALVKKLIDLPRSLGVPEDAKDFAADAYALSR
                     EPIRGELDEYEDFGNRRLRTVGELIQEAFRVGLYRMERVVRERMTTEDVDTITPQTII
                     NIRPVVAALKEFFGSSQLSQFMDQTNSLSGLTHRRRLSALGAGGLTRERAPIEVRDVH
                     PTHYGRMCPIETPEGPNIGLQGSLASMATVSEFGFVQTPYRVVKNGKVTDEIVYLDAS
                     EEEKLTIAQANEPVDEKSGKFVNDTVLCRGPGGEPVTAVPKEIQYMDVNPAQIVSVGT
                     ALIPFLEHNDANRALMGANMQRQAVPLLITEAPLVGTGLEYRAAVDTGDVVVAKRAGT
                     VTDVSAERIVVEAKDGIDEYELVKFMRSNQGTIMHQKPIVGLNEKVKEDQVLADGSST
                     AQGELALGKNLLVAFMPFEGYNFEDAIVISEKLVKDDMLSSIHIHEYEIDARATKLGD
                     EEITRDIPNRSEESLRDLDDRGVVRIGAEVGSGDLLVGKVTPKGETELTAEEKLIRAI
                     FKEKAREVRDTSLKVPHGEGGKVIDVKTFSREAGDDLSPGVNELVRVYVAKKRKIAEG
                     DKLAGRHGNKGVIAKIVPEEDMPFLEDGTPVDVVLNPLGVPSRMNIGQILETHLGWAA
                     AHGVFAENGESNGPTPIATPVFDGATEAEVDQALVDWTKQNPDHPIKFKVDESRRAGR
                     MCSGKARLFNGRTGEPFDQKVTVGFMYILKLLHLVDDKIHARSTGPYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTIKSDDT"
BASE COUNT         1373 a         2564 c         2710 g          915 t
ORIGIN      
        1 gaccagcaga agctcaaggt gcaggtgaac atcttcgagc gccaggtgcc ggtcgagctc
       61 ggcttcgacc aggtcaagaa gatcgactga gaaggaagcg atggcaaaga aggttctgac
      121 actcatcaag ctccagatcc ccggcggcca ggcgaacccg gccccgccgg tcgggcccgc
      181 gctcggccag cacggggtca acatcatgga gttctgcaag gcgttcaacg ccgagaccgc
      241 gcaggcgggc ggccggatca cgccggtcga gatcaccgtc ttcgaggacc gctcgttcac
      301 gttcatcacg aagacgccgc cggccgccgt cctcatcaag gaggcgctga acatcgagaa
      361 gggctcgggc gagccgaacc gcgagaaggt cgggaggctg acgcgcgacc aggtgcgcca
      421 gatcgccgag acgaagctcg tcgacctgaa cgcgcgcgac gtcgacgagg cctccaaggt
      481 gatcgccggc acggcccgct cgatgggcgt cgaggtggac ctgtgagcgc gcacggcaag
      541 cgctaccgcg acgcccgcgc cgcgatcgac cgcgagcggc tctactcgcc gctcgaggcg
      601 atccgcctgc tcaagggcgc cggcgagacc aagttcgacg agacggtcga ggtgcacttc
      661 aacaccggcc tgaacgtccg ccacgccgag cagcagctcc gcggcaccct catgctcccg
      721 cacgggaccg gcagcgacgt gcgcgtcgcc gtcttcgccg agggcgagaa ggcgaaggag
      781 gcgcaggacg ccggggccga cgtcgtcggc gcggccgacc tcgcgacgca gatcgaggag
      841 ggcttcgacg acttcgacct cacgatcgcg accccggaca tgatgggcac ggtcgggaag
      901 ctcggccgca tcctcggccc gcgcggcaag atgccgaacc cgaaggcggg cacggtgacg
      961 ttcgacgtgg ggaaggccgt ctcggacgca aaggccggga agctcgaata ccgcaccgac
     1021 cgcggcgcga acgtccacct ctcgatgggg aagcgcagct tcgacgagcg cgcgctgctc
     1081 gagaactacg cggcgatcct cgacgagatc atgcgggcga agcccgcggc cgcgaagggc
     1141 cgctacatcc gctcggtcac catcacgagc tcgatgggcc cgggcatcaa gatcgacccg
     1201 gcgaggacgc gcgacctcct cgcggagctc gaggaggagc cccagccggc cgtcgcgtag
     1261 acacgtaccc cgcccgccag agacagccgg cagcgcgtca gcgcggaaga ccagccgagg
     1321 cggcgcgaag agcttcgcct cctcgtgccc gccctgcggg cacgaaccgt tttcggagga
     1381 ggtgaacgaa tgctgaaaca ggagaaggag cgcgtcgtcg cggagctcgc cgagcggctg
     1441 caggcgtccg agaccctgct cgtggccgac taccgcggcc tcgacatggt ggagatcgac
     1501 gccgtccgca ccgagctcat caagcacggc gcgcgcttca cggtcgtgaa gaacacgctc
     1561 acgaagcggg ccgccgagca ggctggcgtc ccggagctga acgagttcct cgacggcccg
     1621 acggcgatcg cgttcgtcgg cgacggcgac atggtcgcgg tcgcgaaggc gctgaacgac
     1681 tcggcgaagc agacgaagat cctcgcgctc aagggcggga tcctcgccgg caagccgatc
     1741 agcgccgacg acgtgcgcga gctggcggcg ctgccgccga tggagcagct ccgcggccag
     1801 gtgctcggcg cgatcgtcgc gccgctgaac gcgatcgcgg ggctcgtgaa cgcgccgctc
     1861 cagaacctgc tcggcctcgt cgacgcgagg atcgagcagc tgcgcgagca gggcgacgcg
     1921 ggcgaggcgg ccgccgaacc gcaggccgag gagcccgccg cggaggagcc ggcgcccgac
     1981 gaggagcccg ccgccgagga ggctccggcc gccgaggcga gcgaagagac cgaaacggaa
     2041 tccagcgaag agacgaacga gaacgaggag gagtgatggc tgcaagcgga gttgacaagg
     2101 tgttcgacga gctcgggaag atgagcgttc tcgacctcgt cgagctgaag aacaagatcg
     2161 aggaggagtg gggcatcacg gccgccgctc ctgtcgcagt cgcggcgccc ggcgccgcgg
     2221 ccccgggcgc ggccgacggc ggcgccgccg aggagaagtc ggagttcgac gtcgtcctca
     2281 ccgcggccgg cgacaagaag atccaggtga tcaaggtcgt ccgcgcgatc acgggcctcg
     2341 ggctcaagga ggccaaggac ctggtcgacg gggcgccgaa cccgatcaag gagggcgtgc
     2401 cgcaggacga ggccgactcg atcaagagcc aggtcgagga ggccggcggc tcggtcgagg
     2461 tcaagtagcg ccgacccgtg tctcgctcac gagggcccgc cgcgaggcgg gccctcggcg
     2521 ttggtgtgat gcgtctcccg tcggcggtgt tggcccgacg tcccccatcg tggcgattgc
     2581 gtcgtgttcc ccatctcgcc gacaatgcga ggcatggcag cccgcgccac gatccgctcg
     2641 ctcgcgaggc tcggctggac ggccgcgctc gtcggcctcg ccgtgctcgt cgtcgcggcc
     2701 ccggcgtcgg cgaagggccc gccgagcggc aagggcgatc cgcccccgtg ggccggcggc
     2761 tcgggcggca agccgagcgg cggcccgcct ccgtgggccg gcggtagcgg caagagcgcg
     2821 gctgccgagc aggcgaaggg cgccgcgaag aaggccgaga agggcctccg caaggcggag
     2881 aagaaggcgg ccaaggcgcg cgagggcgcc gagcagaacc cggcattcac ctgcttcgcc
     2941 ctgatcggcg agctcggcga ggagttctac gagacgtacg ggacgaaccc gaacaaggcg
     3001 aacgccttcg ggaagtgcgt ctcggagcac gcccgcgagc agggcgacgg cgacgaggag
     3061 gaggccgggg acgagccggc cgagtgcgag gagccggccg agagcgaaga gccggccgag
     3121 ggcgatggcg acggcgaggg cgatgagccc gccggcgagg aggatccgct cgcggagctc
     3181 gcctcgctcg acgagcccga gccggagccg tccgagggcg agtgcgaccc ggccggcgag
     3241 gacacgggcg aggaaggcga ggagaccgaa ggcgccgagg ccggcgagcc ggagaccgag
     3301 ggcgagaccg agggcgccgc ggaggccgag acagggctca ggtgcttcct tccgctcgcc
     3361 gcgcgcgcga acccgttcgc cgtctgcgcc cgcacgtagc gccggcccgc acccgttcga
     3421 gggacggttt gtgagcgggc tgtaagacct gctcccccgt ccgagtggaa cgcgtggagc
     3481 ggcctcgggg cgcgaccgac actcgcggcg tacacgacct ccaggaggag aggatgaacc
     3541 ggaagctcac cgccgcgctg ttcgtcgtcg ccgcgctctc cgtcgtcgcc gccccgcagg
     3601 cgctcgcggg cggcggcaag aagcccgcga agaacgccgc gaaggcgacc tgcaaggccg
     3661 agcgcgccgc gctcgggacg aggctcttcc gcgcgaccta cggcgcgacg cccgtcgcga
     3721 cgtgccgccg ccgcgtgctg gacgaggcgc aggaggcggt cggcaacgct gctcagcagt
     3781 gcttcgccga gcgcgcggcc gacgcggacg cgttccgcga gaagtacggg cagaacgcaa
     3841 acaagcggaa cgcgttcggg aggtgcgtct cgacgctcgc gaagaaggag cttgcggagg
     3901 aggtcctcga gacgctgaac gcggccaagg agtgcaaggc tgaacggggt gaggatccgg
     3961 acgcgttccg cgagaagtac ggcacgaacg cgaacaagtc gaacgccttc ggtaagtgcg
     4021 tctccggcaa gaaggccgcc gagcccggcg aggaagcgcc gggcgagggc gaaggcgagg
     4081 gcgagggcga ccccggcgag ggtggcggcg cggaggtcta gcccgagagc ccgaacgacg
     4141 cgctgcgcgc gcgaggggcg tcccggcggg ggcgcccctt ctcatttccg ccgcgcgacg
     4201 gcgcggcctg gcctcccgcc gctatactgc tccggacggc gatccccgcg ccgtttcatc
     4261 ttgcgggaga ctcggtcgac ctgctacctt agaaggtcgc gccgctttct gctgcattcg
     4321 tcccggttcg tgtactacga ccctgcctag aggaggtcgc cctacgtggc caccaaggtc
     4381 gccccgcgcc ccgcgcggaa gagcttttcc cgtctgaagc acgtcctcga ccttcccaac
     4441 ctcatcgaca tccagcgagc ctccttcgac tggttcatga aggaagggct tcgcgagacg
     4501 atcgacgaca tctcgccgat cgaggactac accggcacgc tcgccgtcga gttcgccgac
     4561 tactggttcg aggacccgcc catctcgatc aaggagtgcc gtgagaagga cctgacgtac
     4621 caggctccgc tccacatgac cgtccgcttc gtgaacacgg agacgggcga gatccgcgag
     4681 cagaacgtct tcatgggcga cttccccatg atgacggagt ccggcacgtt catcatcaac
     4741 gggaccgagc gcgtgatcgt cacgcagctc gtccggtcgc ccggcgccta cctcatggag
     4801 ccgaaggatc cggacaagca ggtcttcatc gcgaacctca tgccgagccg cggctcctgg
     4861 ctcgaggtcg agatcgacaa gaagggcgtc gtctacgcgc gcatcgaccg gaagcgcaag
     4921 ctcccgatca cgacgctcct gcgcgccctg cccgccgagg acccgaccac cgggttcgag
     4981 ctcgacacgt cctcgaacga gaagatcctc gagctcttcg acaactcgcc gttcatccag
     5041 ctcacgctgg acaaggacac gaccacgcgc gaggaggagg cggtcgtcga ggtcttcaag
     5101 aagcagcgcc ccggcgagcc gcccacgctc gagaacgccc gcaacctcct gcgcgggctc
     5161 ttcttcgatc cgaagcgcta cgacctcacg aaggtcggcc gctacaagct gaaccagcgg
     5221 ctcgaggtcg acgtgccgga cgacgtccgc gtcctcacga cgcaggacat cgtcgcgctc
     5281 gtgaagaagc tgatcgacct gccgcggagc ctcggcgtgc ccgaggacgc gaaggacttc
     5341 gcggccgatg cgtacgcgct ctcgcgcgag ccgatccggg gtgagctcga cgagtacgag
     5401 gacttcggca accgccggct ccgcacggtc ggcgagctga tccaggaggc gttccgggtc
     5461 gggctctacc ggatggagcg cgtcgtccgc gagcggatga cgacggagga cgtcgacacg
     5521 atcacgccgc agacgatcat caacatccgg cccgtggtcg cggcgctgaa ggagttcttc
     5581 ggctcctcgc agctctcgca gttcatggat cagacgaact cgctctccgg gctcacgcac
     5641 cggcggcggc tctcggcgct cggcgccggc ggcctcacgc gcgagcgcgc gccgatcgag
     5701 gtgcgcgacg tccacccgac ccactacggg cgcatgtgcc cgatcgagac cccggagggg
     5761 ccgaacatcg gcctgcaggg ctcgctcgcg tcgatggcga ccgtgtccga gttcggcttc
     5821 gtccagacgc cgtaccgggt cgtcaagaac ggcaaggtga cggacgagat cgtctacctc
     5881 gacgcgagcg aggaggagaa gctcacgatc gcgcaggcga acgagccggt ggacgagaag
     5941 agcgggaagt tcgtgaacga caccgtcctg tgccgcgggc ccggaggcga gccggtcacg
     6001 gccgtgccga aggagatcca gtacatggac gtgaacccgg cgcagatcgt ctccgtcggg
     6061 acggcgctca tcccgttcct cgagcacaac gacgcgaacc gcgcgctcat gggcgcgaac
     6121 atgcagcggc aggccgtgcc gctcctcatc accgaggcgc cgctcgtcgg caccgggctc
     6181 gagtaccggg ccgcggtcga caccggcgac gtcgtcgtcg caaagcgggc ggggacggtc
     6241 accgacgtga gcgccgagcg gatcgtcgtc gaggcgaagg acgggatcga cgagtacgag
     6301 ctcgtcaagt tcatgcgctc gaaccagggc acgatcatgc accagaagcc gatcgtcggc
     6361 ctgaacgaga aggtgaagga ggaccaggtc ctcgccgacg gcagctcgac cgcgcagggc
     6421 gagctcgcgc tcggcaagaa cctgctcgtc gccttcatgc cgttcgaggg ctacaacttc
     6481 gaggacgcga tcgtcatctc ggagaagctc gtgaaggacg acatgctgtc gtccatccac
     6541 atccacgagt acgagatcga cgcgcgcgcg acgaagctcg gcgacgagga gatcacgcgc
     6601 gacatcccga accgctcgga ggagtcgctc cgcgacctcg acgaccgcgg cgtcgtgcgg
     6661 atcggggccg aggtcggctc cggcgacctg ctcgtcggca aggtcacgcc gaagggcgag
     6721 accgagctga ccgcggagga gaagctcatc cgcgcgatct tcaaggagaa ggcgcgcgag
     6781 gtgcgcgaca cctccctcaa ggtgccgcac ggcgagggcg gcaaggtgat cgacgtgaag
     6841 acgttctccc gcgaggcggg cgacgacctc tccccgggcg tgaacgagct cgtccgcgtc
     6901 tacgtggcga agaagcgcaa gatcgccgag ggcgacaagc tcgccggccg ccacggcaac
     6961 aagggcgtga tcgcgaagat cgtccccgag gaggacatgc cgttcctcga ggacgggacg
     7021 ccggtcgacg tcgtcctgaa cccgctcggc gtgccgagcc ggatgaacat cggccagatc
     7081 ctcgagaccc acctcgggtg ggcggccgcc cacggcgtct tcgccgagaa cggcgagagc
     7141 aacggcccga ccccgatcgc gacgcccgtc ttcgacggcg cgacggaggc ggaggtcgac
     7201 caggcgctcg tggactggac gaagcagaac cccgaccacc cgatcaagtt caaggtggac
     7261 gagagccgcc gcgcgggccg catgtgctcg ggcaaggcgc ggctcttcaa cggccgcacc
     7321 ggcgagccct tcgaccagaa ggtcacggtc ggcttcatgt acatcctgaa gctcctgcac
     7381 ctcgtggacg acaagatcca cgcgcgctcc acgggcccgt actcgctcgt cacgcagcag
     7441 ccgctcggcg gaaaggcgca gttcggcggc cagcgcttcg gcgagatgga ggtctgggcg
     7501 ctcgaggcgt acggcgccgc gtacacgctg caggagatgc tcacgatcaa gtccgacgac
     7561 ac
//
LOCUS       DASTFC010000384         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_658917,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000384 DASTFC010000000
VERSION     DASTFC010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658917"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..245)
                     /locus_tag="VFO88_11255"
     CDS             complement(102..245)
                     /locus_tag="VFO88_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546143.1"
                     /translation="MAEGESGGEPCVVVLVEIATEELGRALPTDLGGHPVEIRETGTF
                     EAL"
     gene            complement(319..1389)
                     /locus_tag="VFO88_11260"
     CDS             complement(319..1389)
                     /locus_tag="VFO88_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546144.1"
                     /translation="MVKRAWTLLTTLALATGLLAAAAAAQGPTAPPGLARALAAQERA
                     TAALLDRPGVVGTAVAVENGRPVVAVLTARSGVAGLPRSVDGVDVVVRVTGPIKALHH
                     RPGHGGGPPTSPPPPPPPPPPPPPGGGTDPKARFDRPVPIGVSTGNEGECSAGTIGAR
                     VRSGTTVYALSNNHVYARENRAPLGSRVLQPGRLDTGCTIDANNVLGTLSAFVPITMS
                     TTASNRVDAAIAVSDTARLGNSTPANGYGTPQSATVSAAVGQSVQKYGRTTGHTQGTI
                     TMVNATVNVSYSTGTARFVGQVIVESSSTFIQPGDSGSLLVTSNRNPVGLLFAGSSNG
                     RLAVANRIQDVLSALGVSVDGS"
     gene            complement(1482..>4024)
                     /locus_tag="VFO88_11265"
     CDS             complement(1482..>4024)
                     /locus_tag="VFO88_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9546145.1"
                     /translation="QLLAGAAALARPLFEPGGEHPSAGEDLELLHGLYWLTANLAERS
                     PLVLVVDDLHWGDPSSLRFLTYLAPRLESLPVAVLVSLRSSGVADGDGLLQRLVGHPA
                     AQTVRVQPLSPAGVETLVRERHPGTDAAFCAACTRASGGNPHLVREVLAALAAEGRAP
                     DETSADAVAELAPESVLRSVLDRLARLPFAASALARALAVLGDGAQLRHAAALAELDV
                     EEAASAADRLASVEILAPGEPLAFLHPLLRSAVYADLPPVERGLLHKRAAEILADEAP
                     PERVALHLLAAPATGDPRAVSILRAAARRARAAADPEAAARYLARARDEPPPRGALAG
                     VLVELGEAEAAAGAETALARLEEALALLEDPTERAQTRLRLGWMLHKSGRIREAADVF
                     QAGLDEVRPASPHARALEVGYLGVAWYDSRRVADVVARRRALLSAAEGKPGQAERGLL
                     AQQLLHELSAGESHEEVRALARRLLDDGALLEEEGADSLNVWIAVGALSWSDATEEAE
                     AAIAAALEDTGRRGDYLSGALALYTRAWPRFWSGRIADALADAQAAVSAWQGAWAMYL
                     PGAMYWLARAHIERDELDEAEAALELPSVEERWGGTTMYAAWRAARGRVALARGRAED
                     ALDEQLASGALLRDTLGLRNPAIGEWRSEAALAAVRLGRDDLARELVDEELELARRFG
                     AARPIGVALRGAGLVRGDAELVREAVTVLEGSPARLELARALVDLGALLRRSGSRADA
                     REPLRRGLELAERFGAFRLERQAREELAATGARLGKRSFAGRDALTPSERRVAEMAAS
                     GMSNREIAQSLFVTVNAVKWHLRNAYRKLDIATREELGAALAEEASRA"
BASE COUNT          471 a         1544 c         1538 g          471 t
ORIGIN      
        1 gaggcgttcg agcaccgcgg cgtcaaggcg acgctcgtcc tcggcgaaac gggctcgatc
       61 ccgctcgcct aggcgcacgg cctcgacgcc gccggtccct cctagagcgc ctcgaacgtc
      121 ccggtctcgc gaatctcgac cgggtgcccc ccgaggtcgg tgggaagcgc gcgcccgagc
      181 tcctcggtcg cgatctcgac gagcacgacg acgcacggct cgccgccgga ctcgccctcg
      241 gccacgccga cgacgccggg gatggagagg agctcgcccg cgtgctcgac gagctcctct
      301 ccgaccgacc gttccgggtt aggagccgtc gacgctcacc cccaaggcgc tcagcacgtc
      361 ctggatccgg ttcgccacgg cgagccgccc gttcgagctt ccggcgaaca gcagcccgac
      421 cggattgcgg ttcgaggtca cgaggagcga ccccgagtcg cccggctgga tgaacgtgct
      481 cgagctctcg acgatcacct ggccgacgaa gcgtgccgtc cccgtgctgt agctcacgtt
      541 cacggtcgcg ttcaccatcg tgatcgtccc ctgcgtgtgg ccggtcgtcc ggccgtactt
      601 ctggacggac tggccgaccg cggcgctcac cgtcgccgac tgcggcgtgc cgtagccgtt
      661 cgccggcgtc gagttcccga gacgcgccgt gtcgctcacc gcgatcgccg cgtcgacgcg
      721 gttgctcgcg gtcgtcgaca tcgtgatcgg cacgaacgcc gagagggttc cgaggacgtt
      781 gttcgcgtcg atcgtgcagc cggtgtcgag gcgccccggc tggagcacgc ggctgccgag
      841 cggcgcgcgg ttctcgcgcg cgtagacgtg gttgttgctc agggcgtaca ccgtcgtccc
      901 ggagcgcacc cgcgcgccga tcgtgccggc ggagcactcg ccctcgttcc cggtcgagac
      961 gccgatcggg accgggcggt cgaagcgcgc cttcgggtcg gtcccgccgc cgggcggagg
     1021 cggaggaggc ggcggaggcg gcggcggtgg cgaggtcggc gggccgccgc cgtggcccgg
     1081 acggtggtgc agtgccttga tcggcccggt cacccggacg acgacgtcga cgccgtcgac
     1141 cgaccgcggc aggccggcga cgccgcttcg ggccgtgagc accgcgacga cgggtcgtcc
     1201 gttctcgacc gcgaccgcgg tcccgacgac accgggccgg tcgaggagcg ccgccgtcgc
     1261 gcgctcctgc gcggcgagcg cgcgggcgag gccgggcggc gcggtgggcc cttgtgccgc
     1321 ggccgccgcg gcgagcaggc cggtggccag cgcgagagtc gtgagaagcg tccacgctcg
     1381 cttcaccatc tcgtgtcacc cccatggggt cggagcgccg cgccgcaccg acgctggtct
     1441 gctgcggacc tgccgagggt accgcgttct gcgcccggcc gctacgcccg gcttgcctcc
     1501 tccgcgagcg cggcccccag ctcttcccgc gttgcgatgt cgagcttccg gtacgcgttg
     1561 cggaggtgcc acttgaccgc gttcacggtc acgaagagcg actgggcgat ctcccggttg
     1621 ctcatcccgg acgcggccat ctcggcgacg cgtcgctcac tcggcgtcag ggcgtcgcgg
     1681 ccggcgaagg agcgcttccc gagccgcgcg cccgtcgcgg cgagctcctc gcgggcctgg
     1741 cgctcgagcc ggaacgcgcc gaaccgctcc gcgagctcga gcccgcgacg gagcggctcg
     1801 cgggcgtcgg cgcggctccc gctccggcgg aggagggcgc cgaggtcgac gagcgcgcgg
     1861 gcgagctcga gccgggcggg cgagccctcg aggaccgtga cggcctcgcg aacgagctcg
     1921 gcgtcgccgc gcacgagccc cgcgccgcgg agcgcgaccc cgatcggccg cgccgccccg
     1981 aaccggcggg ccagctcgag ctcctcgtcc acgagctcgc gcgcgaggtc gtcgcgcccg
     2041 agccgcacgg ccgcgagcgc cgcctccgag cgccactcgc cgatcgcggg atttcgcagc
     2101 ccgagcgtgt cgcggaggag cgccccggac gcgagctgct cgtcgagcgc gtcctcggcg
     2161 cgcccgcgcg cgagcgcgac ccgtccccgg gcggcccgcc acgcggcgta catcgtcgtg
     2221 ccgccccagc gctcctccac gctcgggagc tcgagcgccg cctccgcctc gtcgagctcg
     2281 tcccgctcga tgtgtgcgcg cgcgagccag tacatcgcgc cggggaggta catcgcccac
     2341 gcgccctgcc acgcggacac ggcggcctgc gcgtcggcga gcgcgtcggc gatgcgaccg
     2401 ctccagaagc gcggccacgc gcgcgtgtag agggcgagcg cgccgctcag gtagtccccc
     2461 cgccggcccg tgtcctcgag cgcggcggcg atcgcggcct cggcctcctc ggtcgcgtcc
     2521 gaccaggaga gcgctccgac cgcgatccag acgttcagcg agtccgcgcc ctcctcctcg
     2581 aggagcgccc cgtcgtcgag gagccgtcgc gcgagcgcgc ggacctcctc gtgcgactcg
     2641 ccggccgaga gctcgtgcag gagctgctgg gccaggagcc cgcgctcggc ctggcccggc
     2701 ttcccctccg cggcggagag gagcgcgcgc ctccgcgcga cgacgtccgc gacgcgccgg
     2761 ctgtcgtacc acgcgacgcc gaggtagccg acctcgagcg cgcgggcgtg cgggctcgcg
     2821 ggccgcacct cgtcgagccc ggcctggaag acgtccgcgg cctcgcggat ccggccgctc
     2881 ttgtgcagca tccagccgag ccgaagccgc gtctgcgcgc gctccgtcgg gtcctcgagc
     2941 agcgccagcg cctcctcgag ccgcgcgagc gcggtctcgg ctcccgcggc cgcctcggcc
     3001 tcgccgagct cgacgaggac gccggcgagg gcgccccgcg ggggcggctc gtcccgcgcg
     3061 cgggcgagat accgcgccgc ggcctcggga tcggcagccg cccgggcgcg gcgcgcggcg
     3121 gcccgcagga tcgagaccgc ccgcgggtcg ccggtggccg gggcggcgag gagatggagc
     3181 gcgacccgct cgggcggcgc ctcgtccgcg aggatctcgg ccgcgcgctt gtgcaggagc
     3241 ccacgctcga cgggcggcag gtcggcgtag acggccgagc ggaggagtgg atggaggaag
     3301 gcgagcggct cgcccggcgc gaggatctcc acggaggcga gccggtcggc ggccgacgcg
     3361 gcctcctcga cgtcgagctc ggcgagcgcg gccgcgtggc gaagctgcgc tccgtcgccg
     3421 agcaccgcga gcgcgcgggc gagcgccgag gccgcgaacg gaaggcgcgc gagccggtcg
     3481 agcacggagc ggagcaccga ctcgggcgcg agctcggcga ccgcgtcggc gctcgtctcg
     3541 tcgggcgcgc gcccctcggc ggcgagcgcc gcgagcacct cgcgcacgag atgagggttc
     3601 ccaccgctcg cccgcgtgca ggcggcgcag aacgcggcgt cggtcccggg gtgccgctcg
     3661 cggacgagcg tctcgacccc ggccgggctc agcggctgca cgcgcaccgt ctgcgcggcc
     3721 gggtggccga cgagccgctg gagcagcccg tctccgtcgg cgacgccgct cgaccgcagg
     3781 gagacgagca cggcgaccgg caggctctcg agccgcggcg cgaggtacgt gaggaagcgg
     3841 agcgacgacg ggtcgcccca gtggaggtcg tccacgacga gcacgagcgg cgagcgctcg
     3901 gcgaggttcg ccgtcagcca gtagagcccg tgcaggagct cgaggtcctc gcccgccgac
     3961 ggatgctcgc cgcccggctc gaagagcggg cgcgcgagcg cggcggcgcc cgcgaggagc
     4021 tgct
//
LOCUS       DASTFC010000385         5751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_82397,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000385 DASTFC010000000
VERSION     DASTFC010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82397"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VFO88_11270"
     CDS             <1..1611
                     /locus_tag="VFO88_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9546146.1"
                     /translation="EPRHGLPSFRQVLAAIAEAFDGRRGDLDRQADVLTEAIRDASAR
                     APSSEPLSEGLLTGALAALARAFDPVNGGFGGAPKFPPASTLELLLRRGGDEALAMAR
                     LTLDRMAAGGIYDHLGGGFHRYAVDAVWLVPHFEKMLYDNALLARAYLHGWVVTGEER
                     YRRVAEETLDYLLRDLLLPEGGFASAQDADTAGEEGTTYTWTPEELRAVLGADDADGA
                     IRRYGVTEAGNFEGRTVLHLADPDADVPEEARRALLEARMRREQPLRDDKALAAWNGL
                     ALSALAEAARRLERADYLEAANRLADFLLGPLSTPEGDLHRSFRDGLAKIPGYLDDYA
                     AVAAGLLELYVATGELARLEDARRLALRAVELFEDTERGGFFQSPPGSELVARRKELD
                     DNPTPSGNALLADVLVRLARLYGDDELERKAVGVLRLARPLLERAPAAVGALLSVLDL
                     HFAPPRELALVGPPGEPATEELRRAILRPLEPNAVFAFASGPDDPAVGRVPLLEGKGL
                     VDGRPAVYVCERFACRAPVTSAAELAAN"
     gene            1666..2589
                     /locus_tag="VFO88_11275"
     CDS             1666..2589
                     /locus_tag="VFO88_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546147.1"
                     /translation="MRPIRSLVLLLLAAGALAATAASPAADRPFPQMIALPNGFAPEG
                     IAVGRGDTFYVGSIPTGAIYSGSLRTGEGAVLVPAAPGRQAIGLDVDARNRLFVAGGP
                     TGDAYVYDGATGALLAEYDLASGATFVNDVVVTRDGAYLTDSFNQVLYRIPIAPDGTL
                     GAAAETIPLTGDIAYTTGFNANGIDATPDGRWLVLVQSNTGLLFRVDPATGVAREIDL
                     GGETVTAGDGILLDGRTLYVVRNQQNRIAVVRLAPDLLSGRLVTHLTDPGFDVPTTIA
                     EHGNRLYAVNARFGTPVTPDTEYRVTQLRKR"
     gene            complement(2592..3233)
                     /locus_tag="VFO88_11280"
     CDS             complement(2592..3233)
                     /locus_tag="VFO88_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET9546148.1"
                     /translation="MRHRRPGNRASVSTLVEGRPTLPKLREAAAGCRRCHLWERGTQT
                     VFGEGRARAAVLLVGEQPGNEEDLAGRPFVGPAGRVLDEGLDAAGIDRDAAYVTNAVK
                     HFKWEPKGKRRIHQKPNWTEMTACRPWLEAELQVVKPEVVVALGATAAQTLLGRSFRV
                     TKQRGELVESELHPRVVATVHPSSILRAPDADARRAAMEEFVEDLRVVAKLLG"
     gene            3311..4585
                     /locus_tag="VFO88_11285"
     CDS             3311..4585
                     /locus_tag="VFO88_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546149.1"
                     /translation="MRVFAALVALLVASLGGASAATGQGSWEVWAIDQSNTNGTASGG
                     TIHVYDGPSLAGASAATAAGERIDLGGPAQALCLERTGTAPVRPHMLAFDPSDRYGVV
                     AFVATGHVLFLDTATRAPVECIDVGAQAHAAFPTPDGEAVLVANQNGKLLQRISADFE
                     TGTFALDPAATLNLATCVTPSGAPCEAPGLRPDNAPICPVLDSSGRFAFVTLRGGGLF
                     VVDVRATPLRIVAEYDRATIHPNGCGGVEANGKMYVNSGGGTPANLTELDLYAFRLAD
                     FSLTPGAPNTPAPKLVLSDDEREGADGHGSVLTKHGRYLWMTDRGGSRILIVDTATDR
                     LVNELLLPDHTAPDLLAAAPSGNRIFATLRGSVPLTGDPHVATGSTPGVGVFRVEQGG
                     RAGTFHAVARIANVDAAGLDRADPHALAVRGG"
     gene            4768..5304
                     /locus_tag="VFO88_11290"
     CDS             4768..5304
                     /locus_tag="VFO88_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9546150.1"
                     /translation="MRIAVLGGGRVGSALAATWQAAGHDVAVSTRDTVGEVAAGADVL
                     VLAVPAPAGPEVLAAAGPLDGRILVDATNNLSGGPSGAELAALVPGARVVKAFNTVFA
                     TFMHATPPERPASLVLCGDDTGAKEVVAGLVRDAGFDPVDAGGAERTIDVEAFARLVI
                     NLAYAQGRGPFVYRFEPR"
     gene            5359..>5751
                     /locus_tag="VFO88_11295"
     CDS             5359..>5751
                     /locus_tag="VFO88_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546151.1"
                     /translation="MGHTLRNRLAAGGLAVLLVAGAVAGAVVALRAGSTTVERFALGT
                     VAVQVTPSRQGEVDAYAPLVDWGARVRPYRAPLDVRLEFRALDRDAALASLRSGDAAA
                     ENVRTVQRDLDGVVRDALAHAAIVGGLGS"
BASE COUNT          700 a         2233 c         2088 g          730 t
ORIGIN      
        1 gagccgcgcc acgggctgcc gagcttccgg caggtcctgg cggcgatcgc cgaggcgttc
       61 gacggccggc gcggcgacct cgaccggcag gcggacgtcc tcacggaggc gatccgcgac
      121 gcgagcgcgc gggcgccgtc ctccgagccg ctctccgagg ggctcctgac cggcgcgctc
      181 gccgcgctcg cccgcgcgtt cgacccggtg aacggcgggt tcggcggcgc gccgaagttc
      241 ccgcccgcgt cgacgctcga gctgctcctg cgccgcggcg gcgacgaggc gctcgcgatg
      301 gcccgcctga cgctcgaccg gatggcggcg ggcgggatct acgaccacct cggcggcggg
      361 ttccaccgct acgccgtcga cgcggtctgg ctcgtgccgc acttcgagaa gatgctctac
      421 gacaacgccc tcctcgcgcg cgcgtatctc cacggctggg tcgtgaccgg cgaggagcgc
      481 taccgccgcg tcgccgagga gacgctcgac tacctgctcc gcgacctcct cctgcccgag
      541 ggcggcttcg cctccgcgca ggacgcggac accgcgggcg aggaggggac gacgtacacg
      601 tggacgccgg aggagctccg cgcggtgctc ggcgccgatg acgccgacgg ggcgatccgc
      661 cgctacggcg tcacggaggc ggggaacttc gaggggcgca cggtgctcca cctcgccgac
      721 cccgacgcgg acgtcccgga ggaggcgcgc cgcgcgctgc tcgaggcacg gatgcgccgc
      781 gagcagccgc tccgcgacga caaggcgctc gccgcctgga acggcctcgc gctctcggcg
      841 ctcgcggagg cggcgcgccg gctcgagcgc gccgactacc tcgaggcggc gaaccggctc
      901 gcggacttcc tgctcggccc actctcgacg ccggagggcg accttcaccg gagctttcgg
      961 gacgggctgg cgaagatccc gggttacctc gacgactacg cggccgtcgc ggccgggctg
     1021 ctcgagctct acgtcgcgac gggcgagctc gcacggctcg aggacgcgcg ccggctcgcc
     1081 ctccgcgcgg tcgagctctt cgaggacacg gagcgcggcg ggtttttcca gagcccgccc
     1141 ggctcggagc tcgtggcgcg ccggaaggag ctcgacgaca acccgacgcc gtcggggaac
     1201 gcgctcctcg cggacgtgct cgtccgcctc gcgcggctct acggcgacga cgagctcgag
     1261 cggaaggcgg tcggcgtgct gcggctcgcg cgcccgctgc tcgagcgcgc tccggccgcc
     1321 gtcggggcgc tcctctccgt cctcgacctc cacttcgcgc cgccgcgcga gctcgccctc
     1381 gtcggcccac ccggcgagcc ggcgacggaa gagcttcggc gcgcgatcct gcgtccgctc
     1441 gagccgaacg ccgtcttcgc gttcgcgtcc gggccggacg acccggcggt cgggcgcgtc
     1501 ccgctcctcg aggggaaggg cctcgtcgac gggcggcccg ccgtgtacgt ctgcgagcgc
     1561 ttcgcctgcc gcgcgcccgt cacgagcgcg gccgagctcg ccgcaaactg aatccgcgcc
     1621 ggccgggtag ggacccccga accccattcg gaccagggag gacacatgcg accgatccgc
     1681 agcctcgtgc tgctcctgct cgcggccggc gcgctcgccg cgaccgccgc gtcgcccgcc
     1741 gccgaccgcc cgttccccca gatgatcgcc ctgccgaacg gcttcgcccc cgagggcatc
     1801 gcggtcggcc gcggggacac gttctacgtc ggctcgatcc cgaccggcgc gatctactcc
     1861 gggagcctcc gcaccggcga gggcgcggtg ctcgtccccg ccgcgcccgg ccgccaggcg
     1921 atcgggctcg acgtggacgc gcgcaaccgg cttttcgtcg ccggcggccc gaccggggac
     1981 gcgtacgtct acgacggggc gacgggcgcg ctcctcgccg agtacgacct ggcgagcggc
     2041 gcgacgttcg tgaacgacgt cgtcgtgacg cgcgacggcg cgtacctgac ggactccttc
     2101 aaccaggtgc tctaccggat tccgatcgcc ccggacggga cgctgggcgc ggccgcggag
     2161 acgatcccgc tcacgggcga catcgcgtac acgaccgggt tcaacgcgaa cgggatcgac
     2221 gcgacgccgg acggccggtg gctcgtgctc gtccagagca acaccgggct cctcttccgc
     2281 gtcgacccgg cgaccggcgt cgcgcgcgag atcgacctgg gcggcgagac ggtgaccgcg
     2341 ggcgacggga tcctgctcga cgggcggacg ctctacgtcg tccgcaacca gcagaaccgg
     2401 atcgccgtgg tgcgcctcgc gcccgacctc ctctcgggcc ggctcgtgac gcacctgacc
     2461 gacccgggct tcgacgtccc gacgacgatc gccgagcacg ggaaccggct ctacgccgtg
     2521 aacgcccgct tcggcacgcc ggtcacgccg gacacggagt accgggtgac gcagcttcgg
     2581 aagcgctgac gtcagccgag gagcttcgcg acgacgcgga gatcctcgac gaactcctcc
     2641 atcgccgcgc gccgggcgtc cgcgtccggc gcgcggagga tggaggacgg gtggacggtg
     2701 gcgacgacgc gcgggtggag ctcgctctcg acgagctcgc cccgctgctt cgtgacccgg
     2761 aacgagcgcc cgaggagggt ctgcgcggcg gtcgcgccga gtgcgacgac cacctccggc
     2821 ttgacgacct ggagctccgc ctcgagccag gggcggcagg cggtcatctc cgtccagttc
     2881 ggcttctggt ggatgcgccg cttgcccttc ggctcccact tgaagtgctt caccgcgttc
     2941 gtcacgtagg cggcgtcccg gtcgatcccg gcggcgtcga gcccctcgtc gagcacgcgc
     3001 ccggcggggc cgacgaacgg gcggccggcg agatcctcct cgttccccgg ctgctcgccg
     3061 acgaggagga cggcggcgcg cgcccggccc tccccgaaca cggtctgcgt gccgcgctcc
     3121 cagaggtggc agcggcggca gcccgcggcc gcctcgcgga gcttcggaag cgtcgggcgg
     3181 ccttccacga gggtggacac actcgcgcgg ttccccggcc ggcgatgcct catgcccggt
     3241 tgcgccggcc gtcgcgggat gccataactt cactttccaa agtcacttcc atccggaaag
     3301 gaagccagcc atgcgcgtat tcgccgccct cgtcgcgctc ctcgtcgcct cgctcggagg
     3361 cgcctccgcc gccaccggcc aggggtcgtg ggaggtgtgg gcgatcgacc agtcgaacac
     3421 gaacgggacc gcgagcggcg ggacgatcca cgtctacgac gggccgtccc tcgccggcgc
     3481 gtcggcggcg acggcggcgg gcgagcggat cgacctcggc ggccccgcgc aggcgctctg
     3541 cctcgagcgg accggcaccg cgccggttcg cccgcacatg ctcgcgttcg acccgtccga
     3601 tcgctacggc gtggtcgcgt tcgtggccac gggtcacgtc ctcttcctcg acacggccac
     3661 gcgggcaccg gtcgagtgca tcgacgtcgg cgcgcaggcg cacgccgcct tcccgacccc
     3721 ggacggcgag gcggtgctcg tcgcgaacca gaacggcaag ctcctccagc ggatctccgc
     3781 cgacttcgag acggggacgt tcgcgctcga ccccgccgcg acgctgaacc tcgccacgtg
     3841 cgtcacgccg agcggggcgc cgtgcgaggc gccgggcctg cgcccggaca acgcgccgat
     3901 ctgcccggtc ctcgactcct cgggccgctt cgcgttcgtc acgctgcgcg gcggcgggct
     3961 cttcgtcgtc gacgtccgcg cgacgccgct ccggatcgtg gccgagtacg accgcgcgac
     4021 gatccacccg aacggctgcg gcggcgtcga ggcgaacggg aagatgtacg tcaactcggg
     4081 cggcggaacg ccggcgaatc tcaccgagct cgacctctac gccttccggc tcgcggactt
     4141 ctcgctcacg ccgggcgcgc cgaacacgcc cgcgcccaag ctcgtgctga gcgacgacga
     4201 gcgggagggc gccgacgggc acggcagcgt cctcacgaag cacggccgct acctctggat
     4261 gaccgaccgc ggcgggagcc ggatcctgat cgtcgacacg gcgaccgacc ggctcgtgaa
     4321 cgagctcctg ctccccgacc acaccgcgcc ggacctgctc gccgccgccc cgtccgggaa
     4381 ccggatcttc gccacgctgc gcggctccgt gccgctcacc ggcgacccgc acgtcgcgac
     4441 gggctcgacc ccgggcgtcg gcgtcttccg cgtcgagcag ggcggccggg ccgggacgtt
     4501 ccacgcggtc gcgcgcatcg cgaacgtcga cgcggccggg ctcgaccggg ccgatccaca
     4561 cgcgctcgcg gtgcgcggcg gctgaggagc cggttgcgcg ggcacggccg atgccataac
     4621 ttcacttccg aaagcaagcc ggactcgttg gccgcgggct ctccgcgcaa caggcgaact
     4681 aacgccgtcc ggtgccgggc gactccccgg gggccggcag cggccggccc cccgcccggc
     4741 tcccctcccg tctcgtaagc tcgggccgtg cgcatcgcgg tgctcggagg agggcgtgtc
     4801 gggagcgcgc tcgcggccac gtggcaggcg gcgggccacg acgtcgccgt ctcgacgcgc
     4861 gacacggtcg gcgaggtcgc ggccggcgcc gacgtgctcg tcctcgccgt gcccgcgccg
     4921 gccgggcccg aggtgctcgc cgcggccggg ccgctcgacg ggcgcatcct cgtcgacgcg
     4981 acgaacaacc tgagcggcgg gccgagcggc gccgagctcg cggcgctcgt ccccggcgcc
     5041 cgcgtcgtga aggcgttcaa caccgtgttc gccacgttca tgcacgcgac gccgccggag
     5101 cggcccgcct ccctcgtcct ctgcggcgac gacaccggcg cgaaggaggt cgtcgccggc
     5161 ctcgtgcgcg acgccggctt cgacccggtc gacgcgggcg gggccgagcg cacgatcgac
     5221 gtcgaggcgt tcgcacggct cgtgatcaac ctcgcgtacg cgcagggccg cggcccgttc
     5281 gtgtaccgct tcgagccccg ctagcccggc tacgatcggt gctgtcggcg cgtcccccgg
     5341 ccgccgatat ccgaggagat gggtcatacc ctgcgcaacc gcctggcggc gggagggctc
     5401 gccgtcctgc tcgtggccgg cgcagtcgcc ggggcggtcg tcgcgctccg cgccggctcg
     5461 acgacggtcg agcgcttcgc gctcgggacg gtggccgtgc aggtgacgcc gtcgcggcag
     5521 ggcgaggtgg acgcctacgc gccgctcgtc gactggggcg cccgcgtgcg cccgtaccgc
     5581 gcgccgctcg acgtccggct cgagttcaga gcgctcgacc gcgacgcggc gctcgcctcc
     5641 ctccgctcgg gcgacgcggc cgcggagaac gtccgcacgg tgcagcgcga cctcgacggc
     5701 gtcgtccgcg acgccctcgc ccacgcggcg atcgtcggcg ggctcgggtc g
//
LOCUS       DASTFC010000386        10193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_1120613,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000386 DASTFC010000000
VERSION     DASTFC010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120613"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFO88_11300"
     CDS             complement(<1..242)
                     /locus_tag="VFO88_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546152.1"
                     /translation="MSVARPLAFLLVLAAVFGGAVSLGWAVGPLERGAASDGEAGHGE
                     EEGEATAATDGPAGLAVAEGGYRLDLVTRSLVAGST"
     gene            complement(239..445)
                     /locus_tag="VFO88_11305"
     CDS             complement(239..445)
                     /locus_tag="VFO88_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET9546153.1"
                     /translation="MTTQELVYSVPGVSCGHCRAAITEEVAPLAGVESVDVDLDAKRV
                     AVRGAELDDAAIRAAIEEAGYEAE"
     gene            688..2316
                     /locus_tag="VFO88_11310"
     CDS             688..2316
                     /locus_tag="VFO88_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9546154.1"
                     /translation="MAADGLGSDGPGRDPEDIFERRLTRTRLLQSAAAAGLALSPALL
                     AACGGGEDGATTAAATESTGPARGGRLRIGHVGAGKGESFNPARGSSFIDASRYYNVF
                     DPLSRVSPTLDVEPGLALEWVPNGDSTEWEIRLRPDVVWHDGKPFTADDVIYTFRQMG
                     DEGHTSHASVLNVRLRDLRKVNDLTLVVPLEAPNARLFDSFVQQNTVIVQDGATDFTN
                     PVGTGPFKFQSFTVGERSLCVRNENYWEEGKPYVDEWEDISIDDNAARLNALLAGEID
                     MMSQLPFAQAREQEGQGEIQVISAESTAIQVFIMAVDQPPFDDVRVRQAFRLIADRQA
                     LIDGALFGYGSLGNDLAGKGLPFFAEDLAPREQDLDEARSLLQQAGQENLTVTLHTSD
                     IVPGFVEAATLFAEQAKGAGVTVNVKREAANAYFDTSLLYTKLDFAQSFWTFSSIPIW
                     YEQALLSDAVWNETHWRDPAFDKLIRDAQGAPDEETATELWRQVQQIQYDEGGYIVWT
                     NQNLVDAARPNVQGVTPSSFFNLGGWNYRDVYLT"
     gene            2354..3370
                     /locus_tag="VFO88_11315"
     CDS             2354..3370
                     /locus_tag="VFO88_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9546155.1"
                     /translation="MTAAADAPALPVARRRRPLVRFLVRRIAAALATLLVASVLVFLG
                     TEVLPGDAASAVLGRTATPEQLEEMQELMGLDRPAYERYLSWLGGLLTGDLGNSAAGY
                     AAGGETPIWEEVAPKLSNSFVLAGITALVMIPLALVLGVLAAVRAGRTLDHTVSVSSL
                     AIISLPEFIIGSLLILVFFSWLDLLPPLSLVPPGESPLARPDALVLPVLTLLGASLAA
                     SIRMVRAGMVEALRSEYVTMARLNGFREGLVVRRYALRNALAPSVQVFAQNIQYLVGG
                     IVVTEYLFNYPGLGKELVDAVAIRDVREVQSIAMLVAAFYLTLNIVADFLVVLLTPKL
                     RTEL"
     gene            3367..4206
                     /locus_tag="VFO88_11320"
     CDS             3367..4206
                     /locus_tag="VFO88_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9546156.1"
                     /translation="MTARLRFLRSATGAVGLGLLALVVGIALLGPLFAPYSPDEPIGI
                     PLTGPAEGQPFGTDFLGRDVLSRALWGGRSVLALAGLATLLAYAGGITIGLVAGHSRS
                     LVDPILMRAVDVLLAFPALLFLLVLVTGAGTSKTVLVLGVALVQMPLVARIVRTATLE
                     QSVRGFVEAAVARGERTAAILRREILPNIVGPLMADVGLRFTYAIILVASVNFLGLGL
                     QPPAADWALIISENRSGLTLNPWVILVPAALISLLTISVNLVGDAVARSLGISTARRR
                     LVR"
     gene            4203..5966
                     /locus_tag="VFO88_11325"
     CDS             4203..5966
                     /locus_tag="VFO88_11325"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9546157.1"
                     /translation="MSGPVLRVEGLSVELDSGEPIVEDVSLELRPGEVLGLVGESGSG
                     KTTTALALLGFARRGARIAGGTVEIDGKPLTGRGEGVARALRGRLVSYVAQDPGAALN
                     PSLRVGDLIGDMLQAHGGDGDLVAGALASVHLPADAEFARRFPHQLSGGQQQRVAIAT
                     ALVCEPRVIVLDEPTTGLDVVTQGRILEEIDRLRRERSVAVVYVSHDLAVVATIADRI
                     AVMYAGQVVEEGPTAAVVARPRHPYTRGLLASVPDVGQPRRIESIPGVAVGVGERPSG
                     CAFAPRCPQKPERAEHEVPPLEPIGEGRRVRCFAWRETPRLAVEELPVPPAAERAVPL
                     LAVEGLRAEYGRLVAVEGVSFELARSECLALVGESGSGKSTIARCVAGLHAPAAGRVL
                     LDGAALAPRAAKRPRAARRRIQIVFQNPNDSLNPRHRVGDAIARPARILRDLPAREAR
                     DEVARLLERVRLPARLAERYPGELSGGERQRVAIARALAAQPDLIVCDEITSALDVSV
                     QAAVLELLAELRTELQLALLFITHDLGVVASVADRVLVLEHGVVCDSGPVDAILRRPE
                     HEYTQRLVAAAPRLPHLSEVA"
     gene            5963..6754
                     /locus_tag="VFO88_11330"
     CDS             5963..6754
                     /locus_tag="VFO88_11330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9546158.1"
                     /translation="MTSVVVVTGAGSGIGRATAERLHADGRAIVGVDRNAEGLEELAA
                     ALGERFAAVTGDVTERATHERAADAAAGQGDLDGWVNSAGIWLKTRAHDFDEADVDRV
                     LAVNLKGSILGCSVACARFLAAGRGGAIVNVSSIEAVTAFPDAIAYQASKGGIDAITR
                     QVAVDYGPAGIRCNGVRPGAIMTPLGDVYLEAYAEEREAMLQSWRDLSPLGLIGQPED
                     VAASVAFLLSEDARFVTGELLDVDGGATARCFRYAPAPDIEERRP"
     gene            6760..7752
                     /locus_tag="VFO88_11335"
     CDS             6760..7752
                     /locus_tag="VFO88_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9546159.1"
                     /translation="MRLEDVARDAGVSKSIASRVLNGDPELLVRPETRERVVAAARRL
                     NYRPHAAARGLKRAETGALGLLVPPLTNPVYVRIIRGAFDRALERDFVVLLVEDVDPE
                     QADATSTALLQAGRIDGLIVASAEPGHPLLATLGRLGIPHVFVNRGVPGSSRNVVVDE
                     AGASAAALAHLVELGHERIGHVAGPQRLDTARRRTAGFLAAAERHGLAVEVEESDFGE
                     TGGAEAAGALLSRVPEATALYTSTLSQAVGAYHTIWRRGLTVPGDLSLVGYDDMPLAE
                     YLRPPLTTVRAPLDELGAVAVDALVDQVLGGEPRDVTIETAPEIVVRASTGAPR"
     gene            7749..8918
                     /locus_tag="VFO88_11340"
     CDS             7749..8918
                     /locus_tag="VFO88_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET9546160.1"
                     /translation="MSAITEVEAIPLRAGRDAGELDSSAETVVVRVTDEDGRTGIGEA
                     DAPALAVRELVLMEGVHGWSRGLREVLLGRDPRDREALWNDLYEATIYHGRRGLGIHA
                     LSAVDVAVHDLAGRQEDRPVYELLGGPCRERCVPYATVWPGTPRGRTLDELMDDTAAL
                     FERALAAGLRAVKMELIFEDAARDEELVECIREGRRLLGEGIALMLDFGYRWRDWRAA
                     HAVLAEVEDCDVFFAEATLHHDDLDGHRELAERLDLRVAGAEMAATVHECREWLERGR
                     VDVLQPDIGRAGGFTEMRRIAALAAEHGALLVPHGWKTGITVAASCHFQAATENVPYV
                     ELLSPALFDSPLRAGLVRPEPELAADGTLSLPPGPGLGVELVAETVERYAVEVPA"
     gene            8915..9655
                     /locus_tag="VFO88_11345"
     CDS             8915..9655
                     /locus_tag="VFO88_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9546161.1"
                     /translation="MSGRLAGRRALLTGATGGIGPVIARAFAAEGARVALVSRSAERL
                     EALAGELDGARAFPADVADRAAVDAAVAEAAGALGGLDTVVNAAAIDTSWHRVGELPV
                     DEWDRAIAVNLSGTFYVCRAALPLLESAGGGSIVNVTSVAGLRAWEEDAAYNASKAGV
                     ELLTRTIAVDYGRAGIRANCLALGIIDAGMTDEITDPAERAALLDRQPAGRIGRPEEA
                     AEAAVWLASDASSFTTGTTLVVDGGFLA"
     gene            9655..>10193
                     /locus_tag="VFO88_11350"
     CDS             9655..>10193
                     /locus_tag="VFO88_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9546162.1"
                     /translation="MRALVWHGGTELSLDELPEPAPGQGQVVFDVEVAGICGSDLHAY
                     RGHPGPRVPPLVLGHEAIGSVEGRAGRFAVFPLVVCGRCAACRRGEENLCAERGLLGL
                     DRQGVFADRVAVREDALVPVPDGVDPRVAVLVEPLAASVSVLRLDRVEEGDRVLVLGG
                     GPIGLLGVWAALRRGAHVT"
BASE COUNT         1212 a         3869 c         3750 g         1362 t
ORIGIN      
        1 tgcgtgctcc cggcgacgag cgagcgcgtg acgaggtcga gccggtagcc gccctcggcg
       61 accgcgaggc ccgccggccc gtccgtcgcc gccgtcgcct cgccttcctc ctccccgtgg
      121 ccggcctcgc cgtcggacgc cgcgccccgc tcgagcgggc cgacggccca gccgagcgac
      181 acggcgccgc cgaagacggc cgcgaggacg aggaggaagg cgagggggcg cgcgacgctc
      241 actcggcctc gtaccccgcc tcctcgatcg cggcgcgaat cgccgcgtcg tcgagctccg
      301 cgccgcggac ggcgacgcgc ttcgcgtcga ggtcgacgtc caccgactcg acgccggcga
      361 gcggggcgac ctcctccgtg atcgcggccc ggcagtggcc gcagctcacg cccgggacgc
      421 tgtagacgag ctcctgcgtg gtcatctgcg gttcctcctt cgatcggacg acgacgcgaa
      481 gaccggcggg acggcgcccg ggccgccacg cgatcgcccg taggaatacc cgaaggcttt
      541 cgcgtcgcgc cgggcgtccc agccggaggg tacgccgccg gacggcctcc gccaagaacc
      601 gcgcccggcg cgtgccttga cttttcttcc gcacggtggg ataaccggcc gcggaaccgg
      661 tttcctagcg ggaggagcgg agcggaaatg gcggcagacg gcctggggag cgacggtccg
      721 gggcgggacc cggaggacat cttcgagcgg cgcctgacgc gcacgcggct gctccagagc
      781 gccgccgccg cggggctcgc cctctcgccg gccctgctcg cggcgtgcgg cggcggcgag
      841 gacggcgcga cgaccgcggc cgcgaccgag agcacggggc ccgcccgcgg cgggcgcctc
      901 cgcatcggcc acgtgggcgc cgggaagggc gagtcgttca accccgcgcg cggctcgtcg
      961 ttcatcgacg cgtcgcgcta ctacaacgtc ttcgacccgc tgagccgcgt cagcccgacg
     1021 ctcgacgtcg agcccgggct cgcgctcgag tgggtcccga acggcgactc gacggaatgg
     1081 gagatcaggc tccgcccgga cgtcgtctgg cacgacggca agccgttcac cgccgacgac
     1141 gtcatctaca cgttccgcca gatgggcgac gagggccaca cgtcgcacgc gagcgtcctg
     1201 aacgtccggc tccgcgacct gcgcaaggtg aacgacctca cgctcgtcgt cccgctcgag
     1261 gccccgaacg cgcgcctctt cgactcgttc gtgcagcaga acaccgtgat cgtccaggac
     1321 ggcgcgaccg acttcacgaa cccggtcggc acggggccgt tcaagttcca gagcttcacg
     1381 gtcggcgagc ggagcctctg cgtgcgcaac gagaactact gggaggaggg caagccgtac
     1441 gtcgacgagt gggaggacat ctcgatcgac gacaacgccg cgcgcctgaa cgccctcctc
     1501 gcgggcgaga tcgacatgat gagccagctc cccttcgcgc aggcgcgcga gcaggagggc
     1561 cagggcgaga tccaggtgat ctcggccgag agcacggcga tccaggtctt catcatggcg
     1621 gtcgaccagc cgccgttcga cgacgtccgc gtacggcagg cgttccggct catcgccgac
     1681 cgccaggcgc tcatcgacgg ggcgctcttc ggctacggct cgctcgggaa cgacctcgcg
     1741 ggcaaggggc tcccgttctt cgcggaggac ctcgcgccgc gcgagcagga tctcgacgag
     1801 gcgcgctcgc tcctccagca ggccggccag gagaacctga ccgtgacgct tcacacgtcg
     1861 gacatcgtcc cgggcttcgt cgaggcggcg acgctcttcg ccgagcaggc gaagggcgcg
     1921 ggcgtcaccg tgaacgtgaa gcgcgaggcg gcgaacgcgt acttcgacac gtcgctcctc
     1981 tacacgaagc tcgacttcgc ccagtcgttc tggacgttct cctcgatccc gatctggtac
     2041 gagcaggccc tcctctcgga cgcggtctgg aacgagacgc actggcgcga cccggccttc
     2101 gacaagctga tccgggacgc gcagggcgcc ccggacgagg agacggcgac ggagctctgg
     2161 cggcaggtgc agcagatcca gtacgacgag ggcggctaca tcgtgtggac gaaccagaac
     2221 ctcgtcgacg ccgcgcggcc gaacgtccag ggggtcacgc cgagctcgtt cttcaacctc
     2281 ggcggctgga actaccggga cgtctacctg acgtgaggcg gacgcctcgc gcccccgccc
     2341 gccggccggc gcgatgaccg cggcagcgga cgcgcccgct cttcccgtcg cgcggcggcg
     2401 ccggccgctc gtccgcttcc tcgtccggcg gatcgccgcc gcgctcgcga cgctgctcgt
     2461 cgcgtccgtc ctcgtcttcc tggggacgga ggtgctgccg ggcgacgcgg cgagcgcggt
     2521 gctcggccgg acggcgacgc cggagcagct cgaggagatg caggagctca tggggctcga
     2581 ccgccccgcc tacgagcgct acctctcgtg gctgggcggc ctcctcaccg gcgatctcgg
     2641 gaactcggcg gccggctacg cggcgggcgg cgagacgccg atctgggagg aggtcgcgcc
     2701 gaagctctcc aactcgttcg tgctcgccgg gatcacggcg ctcgtgatga tcccgctcgc
     2761 gctcgtcctc ggcgtcctcg ccgcggtgcg ggcggggcgc acgctcgacc acaccgtctc
     2821 ggtctcgtcg ctcgcgatca tctcgctgcc cgagttcatc atcgggtcgc tcctcatcct
     2881 cgtcttcttc tcgtggctcg acctcctgcc gccgctctcg ctcgtcccgc ccggcgagag
     2941 cccgctcgcg cgcccggacg cgctcgtcct ccccgtcctc acgctcctcg gggcctcgct
     3001 cgccgcgtcg atccggatgg tgcgcgcggg gatggtcgag gcgctccgct cggagtacgt
     3061 cacgatggcg cggctgaacg gcttccgcga ggggctcgtc gtgcgccgct acgcgctgcg
     3121 gaacgcgctc gcgccgagcg ttcaggtctt cgcccagaac atccagtacc tcgtcggcgg
     3181 gatcgtcgtc actgagtacc tcttcaacta cccggggctc ggcaaggagc tcgtggacgc
     3241 ggtcgcgatc cgcgacgtgc gcgaggtgca gtcgatcgcg atgctcgtcg ccgccttcta
     3301 cctgacgctc aacatcgtcg cggacttcct cgtcgtcctg ctcacgccga agctgaggac
     3361 ggagctgtga cggcgcggct ccggttcctc cgctcggcga ccggcgcggt cggcctcggc
     3421 ctgctcgcgc tcgtcgtcgg gatcgcactc ctcggcccgc tgttcgcgcc gtattcgccc
     3481 gacgagccga tcgggatacc gctcacgggg ccggcagagg ggcagccgtt cgggacggac
     3541 ttcctcggcc gcgacgtcct ctcgcgcgcg ctctggggcg ggcggagcgt gctcgcgctc
     3601 gccggcctcg cgacgctcct cgcctacgcg ggcgggatca cgatcggcct cgtcgcgggc
     3661 cacagccgct cgctcgtcga ccccatcctc atgcgcgccg tcgacgtgct cctcgcgttc
     3721 ccggcgctcc tcttcctcct cgtcctcgtg accggggccg ggacgagcaa gacggtgctc
     3781 gtcctcgggg tcgcgctcgt ccagatgccg ctcgtcgcgc gcatcgtcag gacggcgacg
     3841 ctcgagcagt ccgtgcgcgg cttcgtcgag gcggcggtcg cgcgcggcga gcggacggcg
     3901 gcgatcctcc gccgcgagat cctcccgaac atcgtcgggc cgctcatggc cgacgtcggg
     3961 ctccgcttca cgtacgcgat catcctcgtc gcgtcggtga acttcctcgg gctcgggctc
     4021 cagccgcccg ccgccgactg ggcgctcatc atcagcgaga accggagcgg gctcacgctc
     4081 aacccgtggg tgatcctcgt gccggccgcg ctcatcagcc tgctcaccat ctcggtgaac
     4141 ctcgtcggcg acgcggtcgc ccgctcgctc ggcatctcga ccgcgcggcg gaggctcgtg
     4201 cgatgagcgg gcccgtcctc cgcgtcgagg gcctctccgt cgagctcgac tcgggcgagc
     4261 cgatcgtcga ggacgtctcg ctcgagctcc gccccggcga ggtgctcggg ctcgtcggcg
     4321 agtccggaag cgggaagacg acgacggcgc tggcgctgct cggcttcgcg cggcggggcg
     4381 cgcggatcgc cggcgggacg gtcgagatcg acgggaagcc gctcaccggc cgcggcgagg
     4441 gcgtcgcgcg ggcgctgcgc gggcggctcg tctcgtacgt cgcgcaggat ccgggcgcgg
     4501 cgctcaaccc gtcgcttcgg gtcggcgacc tgatcggaga catgctccag gcgcacggcg
     4561 gcgacggcga cctcgtggcg ggcgcgctcg cgagcgtcca cctcccggcc gacgccgagt
     4621 tcgcacgccg ctttccgcac cagctctccg gcggccagca gcagcgcgtg gcgatcgcga
     4681 ccgcgctcgt ctgcgagccg cgggtgatcg tcctcgacga gccgaccacg gggctcgacg
     4741 tcgtcacgca gggccggatc ctcgaggaga tcgaccggct gcggcgcgag cggagcgtgg
     4801 cggtcgtcta cgtctcgcac gacctcgccg tcgtcgcgac gatcgccgac cggatcgcgg
     4861 tcatgtacgc cggccaggtc gtcgaggagg gcccgaccgc cgcggtcgtc gcgcgccccc
     4921 gtcacccgta cacgcgcggc ctgctcgcgt ccgtccccga cgtcggccag ccgcggcgga
     4981 tcgagagcat cccgggcgtc gcggtcggcg tgggcgagcg gccgtccggg tgcgcgttcg
     5041 cgccccgctg cccgcagaag ccggagcgcg ccgagcacga ggtcccgccg ctcgagccga
     5101 tcggcgaggg gcggcgcgtc cgctgcttcg cgtggcgcga gacgccgcgg ctcgcggtcg
     5161 aggagctgcc cgtgccgccg gccgccgagc gcgcggtgcc gctcctcgcg gtcgagggcc
     5221 tgcgggcgga gtacggccgc ctcgtggccg tggagggcgt gtccttcgag ctggcgcgga
     5281 gcgagtgcct cgcgctcgtc ggcgagtcgg ggagcggcaa gtcgacgatc gcgcgctgcg
     5341 tcgcggggct gcacgccccg gcggcggggc gcgtgctcct cgacggcgcg gcgctcgcgc
     5401 cccgcgccgc gaagcgcccc cgcgcggcgc gccgccggat ccagatcgtg ttccagaacc
     5461 cgaacgactc gctcaacccg cgtcaccgcg tcggagacgc gatcgcgcgg ccggcgcgca
     5521 tcctccgcga cctcccggcg cgcgaggcgc gcgacgaggt ggcacggctc ctcgagcgcg
     5581 tgcgcctccc ggcccgcctc gccgagcgct acccgggcga gctctccggc ggggagcgcc
     5641 agcgcgtcgc gatcgcccgc gcgctcgcgg cccagccgga cctgatcgtc tgcgacgaga
     5701 tcacctcggc gctcgacgtc tccgtccagg cggccgtgct cgagctcctc gccgagctgc
     5761 gcaccgagct ccagctcgcg ctcctcttca tcacgcacga cctgggcgtc gtcgcgtccg
     5821 tcgccgaccg cgtcctcgtc ctcgagcacg gcgtcgtctg cgactccggc ccggtggacg
     5881 cgatcctccg ccggccggag cacgagtaca cgcagcggct cgtcgccgcc gccccgcgcc
     5941 tgccgcacct ctcggaggtc gcgtgacgag cgtcgtcgtc gtcaccgggg cgggctccgg
     6001 gatcggccgc gcgacggccg agcggctcca cgcggacggg cgcgcgatcg tcggcgtcga
     6061 ccggaacgcc gagggcctcg aggagctcgc ggccgcgctc ggcgagcgct tcgccgccgt
     6121 cacgggcgac gtgaccgagc gcgccacgca cgagcgcgcc gccgacgccg ccgccgggca
     6181 gggcgacctc gacggctggg tgaactcggc cggcatctgg ctgaagacgc gcgcgcacga
     6241 cttcgacgag gcggacgtcg accgcgtgct cgcggtgaac ctcaagggct cgatcctcgg
     6301 ctgctcggtc gcctgcgcgc ggttcctcgc cgccgggcgc ggcggcgcga tcgtgaacgt
     6361 ctcctcgatc gaggccgtca cggccttccc cgacgcgatc gcgtaccagg cgtcgaaggg
     6421 cgggatcgac gcgatcaccc gccaggtcgc ggtcgactac gggccggcgg ggatccgctg
     6481 caacggcgtc cggcccggcg cgatcatgac cccgctcggc gacgtctacc tcgaggcgta
     6541 cgccgaggag cgcgaggcga tgctccagtc gtggcgcgac ctcagcccgc tcggcctcat
     6601 cgggcagccg gaggacgtcg ccgcctcggt cgcgttcctc ctctcggagg acgcgcgctt
     6661 cgtgacgggc gagctgctcg acgtggacgg cggcgcgacg gcgcgctgct tccgctacgc
     6721 gccggcgccc gacatcgagg agcggcggcc gtgacccgca tgcgcctcga ggacgtcgcc
     6781 cgcgacgcgg gcgtctcgaa gtcgatcgcc tcgcgcgtcc tgaacggcga cccggagctc
     6841 ctcgtccgcc cggagacgcg cgagcgggtc gtcgccgcgg cgcgccggct gaactaccgc
     6901 ccccacgcgg ccgcccgcgg gctcaagcgc gcggagacgg gcgcgctcgg cctcctcgtc
     6961 ccgccgctca cgaaccccgt ctacgtgcgg atcatccgcg gcgcgttcga ccgcgcgctc
     7021 gagcgcgact tcgtcgtcct cctcgtcgag gacgtcgacc ccgagcaggc cgatgcgacc
     7081 tcgaccgcgc tcctccaggc ggggcggatc gacgggctca tcgtggcctc ggcggagccc
     7141 ggccatccgc tgctcgcgac gctcggaagg ctcgggatcc cgcacgtctt cgtgaaccgc
     7201 ggcgtgccgg ggtcgagcag gaacgtcgtc gtcgacgagg ccggggcctc ggccgccgcg
     7261 ctcgcgcacc tcgtcgagct cggccacgag cggatcggcc acgtcgccgg gccccagcgg
     7321 ctcgacaccg cgcgccgtcg caccgcgggc ttcctcgccg cggccgagcg gcacgggctc
     7381 gcggtcgagg tggaggagag cgacttcggc gagaccggcg gggccgaggc ggccggcgcg
     7441 ctcctctcgc gggtaccgga ggcgaccgcg ctctacacga gcacgctcag ccaggcggtc
     7501 ggtgcgtacc acacgatctg gcggcgcggc ctcacggtgc ccggcgatct gtcgctcgtc
     7561 ggctacgacg acatgccgct cgctgagtac ctgcgcccgc ccctgacgac cgtccgcgcg
     7621 ccgctcgacg agctcggcgc cgtcgccgtg gacgcgctcg tcgaccaggt gctcggcggc
     7681 gagccgcgcg acgtcacgat cgagaccgcg ccggagatcg tcgtccgcgc gtccaccgga
     7741 gcgccgcgat gagcgcgatc accgaggtcg aggcgatccc cctccgcgca ggccgcgacg
     7801 cgggcgagct cgacagctcc gctgagacgg tcgtcgtgcg ggtcacggac gaggacggcc
     7861 gcacggggat cggcgaggcc gacgcgcccg cgctcgccgt ccgcgagctc gtcctcatgg
     7921 agggcgtcca cggctggagc cgcggcctcc gggaggtgct gctcggacgc gacccgcgcg
     7981 atcgcgaggc gctctggaac gacctctacg aggcgacgat ctaccacggc cgccgcggcc
     8041 tcgggatcca cgcgctctcg gcggtcgacg tcgcggtgca cgacctcgcg gggcggcagg
     8101 aggaccggcc ggtgtacgag ctcctcggcg gcccgtgccg cgagcgctgc gtcccgtacg
     8161 cgaccgtctg gcccgggacg ccgcggggcc ggacgctcga cgagctcatg gacgacacgg
     8221 cggcgctctt cgagcgcgcg ctcgcggccg gcctccgggc cgtgaagatg gagctcatct
     8281 tcgaggacgc cgcgcgcgac gaggagctcg tcgagtgcat ccgcgagggc cggcgcctgc
     8341 tgggagaggg gatcgcgctc atgctcgact tcggctaccg ctggcgcgac tggcgggcgg
     8401 cgcacgcggt gctcgccgag gtggaggact gcgacgtctt cttcgccgag gcgacgctcc
     8461 accacgacga cctcgacggc caccgcgagc tcgccgagcg gctcgacctg cgcgtggccg
     8521 gcgcggagat ggccgcgacc gtgcacgagt gccgcgagtg gctcgagcgc gggcgcgtcg
     8581 acgtcctcca gcccgacatc gggcgcgccg gcggcttcac cgagatgcgg cgcatcgccg
     8641 cgctcgccgc cgagcacggc gcgctcctcg tcccgcacgg ctggaagacg gggatcaccg
     8701 tggcggcgtc ctgccacttc caggcggcga ccgagaacgt cccgtacgtc gagctcctct
     8761 cgcccgccct cttcgactcg ccgctccgcg ccgggctcgt gcggcccgag ccggagctcg
     8821 ccgccgacgg gacgctgtcg ctcccgcccg gccccgggct cggggtcgag ctcgtcgcgg
     8881 agacggtcga gcgctacgcg gtcgaggtgc ccgcgtgagc ggccggctcg cgggcagacg
     8941 ggcgctcctg acgggtgcga cgggcgggat cggccccgtg atcgcgcgcg cgttcgccgc
     9001 ggagggagcc cgggtcgcgc tcgtctcgcg cagcgcggag aggctcgagg ccctggcggg
     9061 cgagctcgac ggcgcgcgcg cgttcccggc cgacgtggcc gaccgcgcgg cggtcgacgc
     9121 ggcggtcgcc gaggccgccg gcgcgctcgg cgggctcgac accgtcgtga acgcggccgc
     9181 gatcgacacc tcctggcacc gcgtgggcga gctcccggtc gacgagtggg accgcgcgat
     9241 cgcagtgaac ctctccggca cgttctacgt ctgccgcgcc gcgctgccgc tcctcgagtc
     9301 ggccggcggc ggctcgatcg tgaacgtcac gtccgtcgcg gggctgcggg cctgggaaga
     9361 ggatgcggcc tacaacgcct cgaaggcggg cgtcgagctg ctcacgcgca cgatcgcggt
     9421 cgactacggg cgcgcgggga tccgcgcgaa ctgcctcgcg ctcgggatca tcgacgcggg
     9481 catgacggac gagatcaccg atccggccga gcgggccgcc ctcctcgacc ggcagccggc
     9541 cgggcggatc ggcaggccgg aggaggcggc cgaagcggcc gtctggctcg cgtccgacgc
     9601 ctcgtccttc acgacgggaa cgacgctcgt cgtcgacggg gggttcctcg cctgatgcgc
     9661 gcgctcgtct ggcacggcgg gacggagctc tcgctcgacg agctgcccga gccggcgccc
     9721 gggcaggggc aggtcgtctt cgacgtcgag gtcgcgggga tctgcggctc cgacctgcac
     9781 gcgtaccgcg gccacccggg gccgcgcgtc ccgccgctcg tcctcggcca cgaggcgatc
     9841 ggctcggtgg agggccgcgc cggccggttc gcggtcttcc cgctcgtagt ctgcgggcgc
     9901 tgcgcggcct gcaggcgcgg cgaggagaac ctgtgcgccg agcgcgggct cctcgggctc
     9961 gaccgtcagg gcgtcttcgc cgaccgggtc gccgtccgcg aggacgcgct cgtgccggtg
    10021 ccggacggcg tcgatccgcg cgtcgccgtg ctcgtcgagc cgctcgccgc gtcggtgtcc
    10081 gtcctgcggc tcgaccgcgt cgaggagggt gaccgcgtgc tcgtcctcgg cggcgggccg
    10141 atcgggctcc tcggcgtctg ggcggcgctc cggcgcggcg cgcacgtgac gtg
//
LOCUS       DASTFC010000387        10829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gaiellaceae bacterium isolate SMAG_U864 k141_955833,
            whole genome shotgun sequence.
ACCESSION   DASTFC010000387 DASTFC010000000
VERSION     DASTFC010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968871
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiellaceae bacterium (soil metagenome)
  ORGANISM  Gaiellaceae bacterium
            Bacteria; Actinomycetota; Thermoleophilia; Gaiellales; Gaiellaceae.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,284
            CDSs (total)                      :: 2,251
            Genes (coding)                    :: 2,244
            CDSs (with protein)               :: 2,244
            Genes (RNA)                       :: 33
            tRNAs                             :: 32
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Gaiellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955833"
                     /isolate="SMAG_U864"
                     /isolation_source="soil"
                     /db_xref="taxon:2838783"
                     /environmental_sample
                     /country="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFO88_11355"
     CDS             <1..297
                     /locus_tag="VFO88_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546163.1"
                     /translation="EAAAEVTYEYHDEFGHGHCYLTALGDGEEVVVCIAPTDAGSRIA
                     CFTREEAQSTRNAKGLLRLDRQGEVKKQEEWPAECADALAVAAPGTDASGAGGY"
     gene            328..1530
                     /locus_tag="VFO88_11360"
     CDS             328..1530
                     /locus_tag="VFO88_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9546164.1"
                     /translation="MAVEPHARTWEASFAERTRGGAGDAIAAILAFAGATDVISFAGG
                     FPDPKTFPGKALAEIHAELVENGDTSPFQYAPTRGLPGPRDYVASRVERLDGRRPDDP
                     ELLLTSGAIEALELIGKAFLDEGDEVVVEAPTYLGGIMAFESFESRVRGVPLDDEGMQ
                     VDALESLLAGGRAPKLVYTIPDHQNPAGVSLTAERRERLVELARRHGFLLVEDVAYRE
                     LRYSGEQPPSLWSLAPEAVVQIGTFSKTFVPGVRLGWAIGPPEVVARLTWAKQLTDQC
                     AGALGQRLLEEYGRRGLLDRQIAGAIELYRGRRDALLAALERHLAGAARWTRSAGGFF
                     SWVELDPPHEAVAVAERALAAGVAIVPGGPFFPEGGGERHLRISFSLVNEEEIEEGIR
                     RVGALLYP"
     gene            1603..1761
                     /locus_tag="VFO88_11365"
     CDS             1603..1761
                     /locus_tag="VFO88_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546165.1"
                     /translation="MSLLWLLVIVLVILAVAGGAAVHNLLWLVLIVALVLAVFAFMGG
                     RGRTGTTV"
     gene            complement(1824..2279)
                     /locus_tag="VFO88_11370"
     CDS             complement(1824..2279)
                     /locus_tag="VFO88_11370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9546166.1"
                     /translation="MTQATIHTNHGAIELELFDDDAPATVENFRKLAADGFYDGVVFH
                     RVIPDFMIQGGDPTGTGSGGPGYQFDDEANDHGVVRGALAMANAGPNTNGSQFFIVTT
                     ESAPWLDGKHTVFGRVTSGMDVVDAISEVETGPGDRPREDVVMERVELS"
     gene            complement(2276..4024)
                     /locus_tag="VFO88_11375"
     CDS             complement(2276..4024)
                     /locus_tag="VFO88_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HET9546167.1"
                     /translation="MRSAARNRWEWTLVVVAAAVALVLGMIGFARLDPDAPFTELLYR
                     SLQLFTLESGAVAEGSAPLSLEIARLLAPVVAAYAAVRAVLLLVRDRLSLLRVRLFAR
                     RHAVVAGLGAKGFTLARRLAASGARVVVVERDADNPFVAGCRARRIPVLVGDASDPAV
                     LGRVGAERAAILYVTTGDDRRNIDVGFALDALPVPTRGPRPTVLVHLDDVALWRRLAA
                     RLVGGPRPRPFRVAFFSVRETAARMLVERQPPFPPAGGLPRRATALVVGGSGFGESVI
                     VAAAAAWRRSRPAADEELAIVVAGPAAARERDDIARHHPELEQVCRLVAWEAELDDLA
                     DAPASDAVYVCLPDETEGLVAALALDSARRSGGPIVLAVTDAATGLASALRDRTARLR
                     PFGVLGEALDPEAIGHGTNEILARAKHAHYVASELERGIGPAENPSVVPWEELDESLK
                     ESNRLFADSVGGKLAAAGCAVVPEPLADPEHPSFAFSDAEVEGLARAEHDRWWNDLAA
                     EGWTPGPVKDAAAKVHPSLVPWSELSEEERDKDREPIRSLPAMLAHVGFGITRGVDAA
                     EPETAGRAEVPLGSAG"
     gene            4046..4999
                     /locus_tag="VFO88_11380"
     CDS             4046..4999
                     /locus_tag="VFO88_11380"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HET9546168.1"
                     /translation="MRFLTIEAPGCFLSSERKEAAAMDTESSALSSIFINYRRGDSRV
                     YADMLYTWLSERYGEEQVFKDVDTIEIGLDFDEAIERVVAASDVMLVVIGGDWVTDQS
                     GRRRLEDPEDYVRKEIEAGLARNIRVIPILVESATMPTADDLPDPLMALTRRQAFELN
                     ETRLRSDRDELLRRLDRLMGKTVGNGAAARRSVAAPAPSQPVHEEPTPPPVPSPPPVY
                     PPPQHPPAQAAPASQAAPAYVATPAPDRTKNLRMWGWILAGGSVIIPFAAIGAIILGG
                     LVISRGDGRRTGAGIGIIVTGIFLGLVGLGIWGSFESAAAY"
     gene            complement(4993..5352)
                     /locus_tag="VFO88_11385"
     CDS             complement(4993..5352)
                     /locus_tag="VFO88_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9546169.1"
                     /translation="MPAALTAGSLAVAVWTASGAGELWTGILPAALAAGCTVAAYFLW
                     LRRNRPWHDWTIIGLCLPAIAAAVWIAVGGLVAGVERTDAGRLLFEVAPGVALTGLLC
                     TLVSYHGRHHPDERPGQ"
     gene            5428..6048
                     /locus_tag="VFO88_11390"
     CDS             5428..6048
                     /locus_tag="VFO88_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET9546170.1"
                     /translation="MRLALVPRTTEFYDLFGRAGDNALEAARKAEQRFLEYPNASVSQ
                     ADIKAIEHEGDRITHDLIQLLNTQYVTPFDREDIYELAGAIDDVVDHIDEACDLLELY
                     GVESATRQALQQAEIIVGAVECLNRALHELRGMKGVQKELVELKRLEDEGDRVVREAI
                     ADLFRDPRIDPLVVIRWKDIFDALEASLDACETAANVIGNIVVKNA"
     gene            6054..7166
                     /locus_tag="VFO88_11395"
     CDS             6054..7166
                     /locus_tag="VFO88_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET9546171.1"
                     /translation="METTLLVAVIAAALFFDFTNGFHDAANAIATSVSTRALSPRVAV
                     LYAAVLNFAGAFVFLEVAATVATGIVDAEIVTLEVVLGGVVGAITWNLITWYLGLPTS
                     SSHALIGGVAGSAIAANGFDVVKWGGLLDRVLIPSLLAPILGVAIAFALMLGVLWVIR
                     RQAPGTVNRIFRRLQIVTAGFVAFTHGTNDAQKTMGIMTLALIAGGHIDADDFHVPTW
                     VIVSAATAMALGTYAGGWRIIRTLGQRIAKLEPPQGFAAEATCAAILLTTGKLGFPVS
                     TTHTISGSVLGAGATRRLTAVRWGVAGNILVAWVMTVPCAALVGAAMESLTRLPGGSV
                     IVFVLAIAIASTAFVARSWQTRKAAVPEPMPVAPPG"
     gene            complement(7170..7796)
                     /locus_tag="VFO88_11400"
     CDS             complement(7170..7796)
                     /locus_tag="VFO88_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET9546172.1"
                     /translation="MTAAASQALPADRDRLVRRARLLAWGSLAWMTAEGVVAVVAGIA
                     AGSIALVGFGLDSAIEGLASVVIIWRFWGARALSERAERRAQKLVAVQFFVLAPYVAF
                     EAVRALVTGHEPESSPLGIGLTIVSAILMPAFGVAKRRVGAALGSAATRAEGMQNMLC
                     AYLSLAVLAGLLGNTLAGWWWLDPLAALLIAYVAVREGREAWRGEECC"
     gene            complement(7825..8235)
                     /locus_tag="VFO88_11405"
     CDS             complement(7825..8235)
                     /locus_tag="VFO88_11405"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9546173.1"
                     /translation="MTDTGQRRPQDVLFVCVHNAGRSQMAAALLAHRGGERVRVRSAG
                     SEPAGEVNPAVVVVMRELGLELAGEQPKRLEDAAVREADVVVTMGCGDACPVYPGKRY
                     EDWELEDPAGKPVETVRRIRDEIDERVRGLLATL"
     gene            complement(8369..9067)
                     /gene="phoU"
                     /locus_tag="VFO88_11410"
     CDS             complement(8369..9067)
                     /gene="phoU"
                     /locus_tag="VFO88_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET9546174.1"
                     /translation="MRLTLQEDLDQLEAALQEEGDLVLRSLRGALNALVDADTELADE
                     VIAFDDEVDARYYAIEEGIQSLLARQTPVAADLRLVLAILHDNLHLERMADYCVTIAK
                     LTKLVEGIAQDATLLEGFEEMGRRLEDMIRVALDSFAARNLEQAESLVHLDDLVDRAN
                     RRVVGHVLELGGDPERREWGLRMIIVSRCLERIGDHAVDIGEQTAYLVTGEFREFTDA
                     SHPGGPPGTAPREL"
     gene            complement(9064..9882)
                     /gene="pstB"
                     /locus_tag="VFO88_11415"
     CDS             complement(9064..9882)
                     /gene="pstB"
                     /locus_tag="VFO88_11415"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET9546175.1"
                     /translation="MTPELLAERAEVRRDTLFGVEDLRVSYAGVPALEGVSLDIYRNG
                     VTAFIGPSGCGKSTFIRCFNRMNDLIPSAQVEGRISYHGVDLYGPDVDPVEVRKRIGM
                     VFQKPNPFPKSIFDNIAFGPRVLRMDGAVDERVERALRRAALWDEVKDRLKESALSLS
                     GGQQQRLCIARCLAVEPDVILMDEPASALDPISTTAIEDLMHELKESYTIVIVTHNMQ
                     QAARVADMTAFFSVKVSDDGKRRTGILVEYDETPTIFTKPADKRTEDYVTGRFG"
     gene            complement(9917..10792)
                     /gene="pstA"
                     /locus_tag="VFO88_11420"
     CDS             complement(9917..10792)
                     /gene="pstA"
                     /locus_tag="VFO88_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9546176.1"
                     /translation="MSAQPVDLRGGDPRERAKGAVFYALLVFSVAVGFVLLGTLFVDV
                     LRKGVLFLDPALFLNPPSFDPLKAGARPAILGTIYLMGLLLVMAVPVGVGTAIYLEEY
                     ANRDRWYNRLLELNIQNLAAVPSIVYGILGLAFLVRGLGLGRVLLAGAMILTLLVLPT
                     IIVASREAIRAVPDSIRQGAYALGSTQWQVISRQVLPAAIPGIATGTILALARAIGET
                     APLLLVGALTYVAFDPTLLGDFTALPIQIYQWIARPQEDFRLLAAAAIIVLLGLLLTM
                     NAFAIWLRNRYQKRW"
BASE COUNT         1538 a         3850 c         3957 g         1484 t
ORIGIN      
        1 gaggcggccg ccgaggtcac ctacgagtac cacgacgagt tcgggcacgg gcactgctac
       61 ctgaccgcgc tcggcgacgg cgaggaggtc gtcgtctgca tcgcgccgac cgacgccggc
      121 agccgcatcg cctgcttcac gcgggaggag gcgcagagca cccggaacgc gaaggggctc
      181 ctccgcctcg accggcaggg cgaggtcaag aagcaggagg aatggccggc cgagtgcgcc
      241 gatgcgctcg cggtcgcggc ccccggcacc gacgcgtcgg gcgccggcgg ctactagccg
      301 gcccgcgctc ctgcgacaat ggggccgatg gctgtcgagc cgcacgcacg cacctgggag
      361 gcgagcttcg ccgagcgcac gcgcggcggg gcgggggacg cgatcgcggc gatcctcgcc
      421 ttcgcgggcg ccaccgacgt catctccttc gcgggcggct tccccgaccc gaagacgttc
      481 ccgggcaagg cgctcgccga gatccacgcg gagctcgtcg agaacgggga cacgagcccg
      541 ttccagtatg cgccgacccg cggcctgccg ggcccgcgcg actacgtcgc gtcccgcgtc
      601 gagcggctgg acgggcggcg gcccgacgac ccggagctcc tgctcacgag cggcgcgatc
      661 gaggcgctcg agctgatcgg caaggcgttc ctcgacgagg gcgacgaggt cgtggtcgag
      721 gcgccgacgt atctcggcgg aatcatggcc ttcgagagct tcgagtcccg cgtgcgcggc
      781 gtcccgctcg acgacgaggg gatgcaggtc gatgcgctcg agtcgcttct cgccggcggg
      841 cgcgcgccga agctcgtcta cacgatcccc gaccaccaga acccggccgg cgtgagcctc
      901 accgccgagc ggcgcgagcg tctcgtcgag ctcgcccggc gccacgggtt cctcctcgtc
      961 gaggacgtcg cctaccgcga gctccgctac agcggcgagc agccgccgag cctttggtcg
     1021 ctcgcgcccg aggcggtcgt ccagatcggg acgttctcga agacgttcgt gcccggcgtg
     1081 cggctcggct gggcgatcgg gccgcccgag gtggtcgcgc gcctcacgtg ggcgaagcag
     1141 ctgaccgacc agtgcgcggg cgcgctgggc cagcggctgc tcgaggagta cgggcgccgc
     1201 ggcctcctcg accgccagat cgcgggcgcg atcgagctgt accgcggccg ccgcgatgcg
     1261 cttctcgccg cgctcgagcg ccacctcgcc ggggcggcga ggtggacccg ctccgccggc
     1321 gggttcttct cctgggtgga gctcgacccg ccgcacgagg cggtcgccgt cgccgagcgg
     1381 gcgctcgcgg cgggcgtcgc gatcgtcccc ggcggcccct tcttcccgga gggcggcggc
     1441 gagcgccacc tccggatctc cttcagcctc gtgaacgagg aggagatcga ggagggcatc
     1501 cgcagggtcg gcgcgctcct ctatccgtag cgctacggag ttttttctcc gcgcagcggg
     1561 aaatgcttcc aggcagtagc gcaaaccgac cgaaggagac gcatgtcgct gctctggttg
     1621 ctcgtgatcg tcctcgtgat cctcgcagtc gccggcggcg ctgcggttca caacctgctc
     1681 tggctcgtgc tcatcgtcgc gctcgtgctc gcggtcttcg cgttcatggg cggcaggggg
     1741 cggaccggga cgaccgtctg atcctgcgcg acgtgtgagc cgcggcccgg cccgcctgga
     1801 cgggggccgg gccgcggcgc gtcctacgag agctcgacgc gctccatgac gacgtcctcg
     1861 cgcgggcggt cgccggggcc ggtctcgacc tcggagatcg cgtcgacgac gtccatcccg
     1921 ctcgtcacgc ggccgaacac cgtgtgcttg ccgtcgagcc acggggccga ctcggtcgtg
     1981 acgatgaaga actggctccc gttcgtgttc gggcccgcgt tcgccatcgc gagcgcgccg
     2041 cggacgaccc cgtggtcgtt cgcctcgtcg tcgaactgat agcccgggcc gccggagccg
     2101 gtgcccgtcg ggtcgccgcc ctggatcatg aagtcgggaa tcacgcggtg aaagacgaca
     2161 ccgtcgtaga acccgtcggc ggcgagcttc cggaagttct cgacggtggc cggcgcatcg
     2221 tcgtcgaaga gctcgagctc gatcgcgccg tggttggtgt ggatcgtggc ctgtgtcatc
     2281 cggcggaccc tagcggcacc tcggcgcggc ccgccgtttc cggctccgcc gcgtcgacgc
     2341 cccgcgtgat cccgaagccg acgtgcgcga gcatcgcggg gagcgagcgg atgggctcgc
     2401 ggtccttgtc gcgctcctcc tccgagagct cggaccaggg gacgaggctc gggtgcacct
     2461 tggccgccgc gtccttcacc gggcccggcg tccagccctc ggcggcgagg tcgttccacc
     2521 agcggtcgtg ctcggcccgc gcgagcccct cgacctcggc gtccgagaag gcgaacgacg
     2581 gatgctcggg atcggcgagc ggctccggga cgaccgcgca gccggccgcc gcgagcttcc
     2641 cgccgacgct gtccgcgaag aggcggttcg actccttcag gctctcgtcg agctcctccc
     2701 acgggacgac ggaggggttc tccgcggggc cgatgccgcg ctcgagctcc gaggcgacgt
     2761 agtgggcgtg cttcgcgcgc gcgaggatct cgttcgtccc gtgcccgatc gcctcggggt
     2821 cgagcgcctc gccgaggacg ccgaagggcc gaagccgtgc cgtgcggtcg cggagggccg
     2881 aggcgagccc ggtcgccgcg tccgtgaccg cgaggacgat cgggccgccg gagcgccggg
     2941 cactgtcgag ggcgagcgcg gcgacgagcc cctccgtctc gtccgggagg cagacgtaca
     3001 cggcgtccga ggccggcgcg tcggcgaggt cgtcgagctc cgcctcccag gccacgaggc
     3061 ggcagacctg ctcgagctcc ggatggtgcc gcgcgatgtc gtcccgctca cgcgccgcgg
     3121 ccgggccggc gacgacgatc gcgagctcct cgtcggcggc gggacgcgag cggcgccagg
     3181 ccgcggcggc ggccacgatc acggactcgc cgaagccgga gccgccgacg acgagcgcgg
     3241 tcgcccgccg ggggagcccg cccgccgggg gaaagggcgg ctggcgctcg acgagcatcc
     3301 gggcggccgt ctcgcggacg ctgaagaacg cgacccggaa cgggcgcggg cgcgggccgc
     3361 ccacgagccg ggcggcgagc cgccgccaga gcgcgacgtc gtccaggtgg acgagaacgg
     3421 tcggccgggg gccgcgcgtc ggcaccggga gcgcgtcgag cgcgaacccc acgtcgatgt
     3481 tccgccggtc gtcgccggtc gtgacgtaga ggatcgcggc gcgctcggcg cccacgcgcc
     3541 cgagcaccgc gggatccgag gcgtccccga cgagaaccgg gatccggcgc gcgcggcagc
     3601 cggcgacgaa ggggttgtcc gcgtctcgct cgacgacgac gacgcgcgcg ccggacgcgg
     3661 ccagccgccg cgcgagcgtg aagcccttcg cgccgaggcc cgcgacgacg gcgtgccggc
     3721 gggcgaagag gcgcacgcgg aggagggaga ggcggtcgcg cacgaggagc agcaccgccc
     3781 gcacggccgc gtacgcggcg accacggggg ccagcaggcg ggcgatctcg agcgagagcg
     3841 gcgcgctccc ttccgccacc gcgcccgact cgagcgtgaa gagctggagc gaccggtaga
     3901 ggagctccgt gaacggtgcg tccgggtcga gccgcgcgaa gccgatcatc ccgaggacga
     3961 gcgcgaccgc cgcggcgacg acgacgagcg tccactccca gcggttgcgt gcggcgcttc
     4021 gcacggtgcg atcgtacgtc agattgtgcg gttcttgaca atcgaggcgc ccgggtgttt
     4081 tctgtcgtcg gagcgcaagg aggccgccgc aatggacacc gagtcgagtg cgctgtcgag
     4141 catcttcatc aactaccgcc gcggggacag ccgggtctac gcggacatgc tctacacctg
     4201 gctgagcgag cggtacggcg aggagcaggt cttcaaggac gtcgacacga tcgagatcgg
     4261 gctcgacttc gacgaggcga tcgagcgggt ggtcgccgcc tccgacgtca tgctcgtcgt
     4321 gatcggcggc gactgggtca ccgaccagag cggccgccgc cgcctcgagg atcccgagga
     4381 ctacgtgcgc aaggaaatcg aggccgggct cgcgcggaac atccgcgtca tcccgatcct
     4441 cgtcgagagc gcgaccatgc cgacggccga cgacctcccc gacccgctca tggcgctcac
     4501 ccgccggcag gcgttcgagc tgaacgagac gcggctccgc tcggaccggg acgagctgct
     4561 gcgccgtctc gaccggctca tggggaagac cgtgggcaac ggcgccgcgg cgcgacgctc
     4621 ggtggccgct cccgccccgt cgcagccggt gcacgaggag cccacgccgc ctcccgtgcc
     4681 ctcgccgcct cccgtgtacc cgccgccgca gcatccgccg gcgcaggccg cgccggcgtc
     4741 ccaggctgcc cccgcgtacg tcgcgactcc tgcgcccgac cggacgaaga acctgcggat
     4801 gtgggggtgg atcctcgccg gcgggtcggt catcatcccg ttcgccgcga tcggggcgat
     4861 catcctcggc gggctcgtga tctcgcgcgg agacggccgg cggaccgggg cgggtatcgg
     4921 gatcatcgtg acggggatct tcctcggcct cgtcggcctc gggatctggg gcagcttcga
     4981 gagcgccgcg gcctactgac cgggccgctc gtccgggtgg tggcgcccgt ggtagctcac
     5041 gagcgtgcag agcagcccgg tcagggcgac gccgggcgcg acctcgaaga ggagccgccc
     5101 ggcgtcggtg cgctcgacgc cggcgacgag cccgcccacc gcgatccaga cggcggccgc
     5161 gatcgcgggc aggcagaggc cgatgatcgt ccagtcgtgc cacggccggt ttcgccgtag
     5221 ccacaggaag taggcggcga ccgtacaccc ggcggccagg gcggccggaa ggatccccgt
     5281 ccagagctcg cccgcgccgg acgccgtcca gacggcgacc gcgagcgagc cggccgtgag
     5341 cgccgcgggc atccgggcga cgatatcgcg cccgccgcgc acggaaccgt gccggacctg
     5401 tgaacgttcg gtgtacgatc gtgcgccgtg cgcctcgccc tcgtaccgcg gacgaccgag
     5461 ttctacgacc tcttcggccg ggccggggac aacgccctcg aggcggccag gaaggcggag
     5521 cagcgtttcc tcgagtaccc gaatgcgagc gtctcgcagg ccgacatcaa ggcgatcgag
     5581 cacgagggcg accggatcac gcacgacctg atccagctcc tgaacaccca gtacgtgacg
     5641 ccgttcgacc gcgaggacat ctacgagctc gccggcgcga tcgacgacgt cgtcgaccac
     5701 atcgacgagg cgtgcgacct tctcgagctg tacggggtcg agtcggcgac gcgccaggcg
     5761 ctccagcagg ccgagatcat cgtcggcgcg gtcgagtgcc tgaaccgcgc cctccacgag
     5821 ctgcgcggga tgaagggcgt ccagaaggag ctcgtcgagc tgaagcggct cgaggacgag
     5881 ggcgaccgcg tcgtgcgcga ggccatcgcc gacctcttcc gcgacccgcg catcgacccg
     5941 ctcgtcgtca tccgctggaa ggacatcttc gacgccctcg aggcctcgct cgacgcctgt
     6001 gagaccgctg cgaacgtgat cgggaacatc gtcgtcaaga acgcctagcg cggatggaaa
     6061 cgaccctgct cgtcgccgtg atcgccgcgg cgcttttctt cgatttcacg aacggcttcc
     6121 acgacgcggc gaacgcgatc gcaacgagcg tctcgacgcg cgcgctctcg ccgcgcgtcg
     6181 ccgtcctcta cgcggccgtc ctcaacttcg ccggcgcgtt cgtcttcctc gaggtcgcgg
     6241 cgacggttgc gacgggaatc gtggacgcgg agatcgtcac gctcgaggtg gtgctcggcg
     6301 gggtggtcgg cgcgatcacc tggaacctca tcacctggta cctcggctta ccgacgagct
     6361 cgagccacgc cctgatcggc ggcgtcgccg ggtcggcgat cgccgcgaac gggttcgacg
     6421 tcgtgaagtg gggcggcctg ctcgaccgcg tgcttatccc gtcgctcctc gcgccgatcc
     6481 tcggcgtggc gatcgcgttc gcgctcatgc tgggcgtcct ctgggtcatc cgcaggcagg
     6541 cgcccgggac ggtcaaccgc atcttccggc gcctccagat cgtgacggcc gggttcgtcg
     6601 cgttcacgca cgggacgaac gacgcgcaga agacgatggg gatcatgacg ctcgcgctga
     6661 tcgcgggcgg gcacatcgac gcggacgact tccacgttcc gacctgggtg atcgtgagcg
     6721 ccgcgaccgc gatggcgctc ggcacgtacg cgggcggctg gcggatcatc cgaacgctcg
     6781 gccagcggat cgcgaagctc gagccgccgc agggctttgc ggccgaggcg acctgcgcag
     6841 cgatcctcct gacgaccgga aagctcgggt ttcccgtctc gacgacgcac acgatctcgg
     6901 gctcggtgct cggcgcgggg gcgacgcggc ggctgaccgc ggtccgctgg ggggtggccg
     6961 ggaacatcct cgtcgcgtgg gtcatgaccg tcccgtgcgc cgcgctcgtc ggcgccgcga
     7021 tggagtcgct cacgcggctg cccggcggca gcgtgatcgt cttcgtcctc gcgatcgcga
     7081 tcgcgagcac ggcgttcgtg gcgcgctcgt ggcagacgcg caaggccgct gtccccgagc
     7141 cgatgcccgt cgcgccgccc ggctaggacc tagcagcact cctcgccgcg ccaggcctcg
     7201 cgtccctcgc ggacggcgac gtaggcgatg aggagcgccg cgagcgggtc gagccaccac
     7261 cagcccgcga gcgtgttccc gaggagcccc gcgagcacgg caagggagag gtacgcgcag
     7321 agcatgttct gcatcccctc ggcccgcgtc gccgccgacc cgagcgcggc gccgacccgc
     7381 cgcttcgcga ccccgaacgc gggcatgagg atcgcgctca cgatcgtcag gccgatcccg
     7441 agcgggctcg actcgggctc gtgcccggtg acgagggcgc gaaccgcctc gaaggcgacg
     7501 tacggcgcga ggacgaagaa ctgcaccgcg acgagcttct gtgcccgccg ctccgcccgc
     7561 tccgagagcg cgcgggcgcc ccagaagcgc cagatgatca cgacgctcgc gaggccctcg
     7621 atcgcgctgt cgagcccgaa gccgacgagc gcaatgctgc cggccgcgat gcccgcgacg
     7681 accgcgacga cgccttcggc ggtcatccac gcgaggcttc cccaggcgag cagccgcgcc
     7741 ctgcggacga ggcggtcgcg atccgccgga agagcctgcg aggccgctgc ggtcacggct
     7801 cgagcgtagc ggcggcgtcg ctccctagag ggtcgcgagc agcccgcgca cgcgttcgtc
     7861 gatctcgtcg cgaatgcggc ggacggtctc gaccggcttc ccggcgggat cctcgagctc
     7921 ccagtcctcg tagcgcttcc cgggatagac cgggcaggcg tcgccacagc ccatcgtcac
     7981 gacgacgtcg gcctcgcgga cggcggcgtc ctcgagccgc ttcggctgct cgcccgcgag
     8041 ctcgagcccg agctcgcgca tgacgaccac gaccgcggga ttcacctcgc cggcgggctc
     8101 gctacccgcc gagcggaccc gtacgcgctc gccgccgcgg tgcgcgagga gcgccgccgc
     8161 catctggctg cgccccgcgt tgtgcacgca gacgaacagg acgtcttgcg gccggcgctg
     8221 tccggtgtca gtcatcgccg agcccttcga atgtggcatg tcatcttcgt cgtcgcgccc
     8281 gacatggcgg gcgcgactcg acgacgtccc gagcgggttt cgccggtcga agtcatcatg
     8341 gccgcaaagc ggccggactt ctgaccggtc aaagctcgcg cggcgcggtg ccgggcgggc
     8401 cgccgggatg cgaggcgtcg gtgaactccc tgaactcgcc ggtcacgagg taggccgtct
     8461 gctcgccgat gtcgaccgcg tggtcgccga tccgctcgag gcagcgcgag acgatgatca
     8521 tccgcagtcc ccactcgcgc cgctcggggt cgccgccgag ctcgaggacg tgcccgacga
     8581 cgcgccggtt cgcgcggtcg acgaggtcgt cgaggtggac cagcgactcc gcctgctcga
     8641 ggttccgggc cgcgaacgag tcgagcgcga cgcggatcat gtcctcgagc cgccgaccca
     8701 tctcctcgaa gccctcgagg agcgtcgcgt cctgggcgat cccctcgacg agcttcgtga
     8761 gcttcgcgat cgtgacgcag tagtccgcca tccgctcgag gtggaggttg tcgtggagaa
     8821 tcgcgagcac gagacgcagg tcggccgcca cgggcgtctg ccgggcgagg agcgactgga
     8881 tgccctcctc gatcgcgtag tagcgcgcgt cgacctcgtc gtcgaaggcg atcacctcgt
     8941 cggcgagctc ggtgtcagcg tcgacgaggg cgttcagcgc gccccggagc gagcggagaa
     9001 cgaggtcgcc ctcctcctgg agcgccgcct cgagctggtc gaggtcttcc tggagggtga
     9061 gtctcatccg aatctcccgg tgacgtagtc ctcggtccgc ttgtcggccg gcttcgtgaa
     9121 gatcgtcggg gtctcgtcgt actcgacgag gatcccggtg cgccgcttcc cgtcgtcgga
     9181 gaccttcacg ctgaagaacg cggtcatgtc cgccacgcgc gccgcctgct gcatgttgtg
     9241 cgtgacgatc acgatcgtgt agctctcctt cagctcgtgc atgaggtcct cgatcgccgt
     9301 cgtcgagatc gggtcgaggg cggaggccgg ctcgtccatc aggatgacgt ccggctcgac
     9361 ggccaggcac cgggcgatgc agagccgctg ctgctgaccg ccggagagcg agagcgcgct
     9421 ctccttcagg cggtccttca cctcgtccca gagcgcggcg cggcggagcg cccgctcgac
     9481 ccgctcgtcc acggcgccgt ccatgcggag gacgcggggg ccgaaggcga tgttgtcgaa
     9541 gatcgacttg gggaacgggt tcggcttctg gaagaccatc ccgatccgct tgcgcacctc
     9601 gaccgggtcg acgtccggcc cgtagaggtc gacgccgtgg tagctgatcc tcccctcgac
     9661 ctgggcggac gggatcaggt cgttcatccg gttgaagcag cggatgaagg tgctcttccc
     9721 gcagccggac gggccgatga aggccgtcac cccgttgcgg tagatgtcga gcgagacgcc
     9781 ctcgagcgcc gggacgccgg cgtacgagac gcgaagatcc tcgacgccga agagcgtgtc
     9841 gcgccgcacc tccgcccgct ccgcgagcag ctcgggggtc acggacgcca ttctcgcacc
     9901 atcggtcact gtcatctcac catctcttct ggtagcggtt gcggagccag atcgcgaacg
     9961 cgttcatcgt gagcaggagc ccgagcagca cgatgatcgc ggccgcggcg aggagcctga
    10021 agtcctcctg cggccgggcg atccactggt agatctggat cgggagcgcc gtgaagtcgc
    10081 cgaggagcgt cgggtcgaac gcgacgtacg tgagcgcgcc gacgagcagg agcggggccg
    10141 tctcgccgat cgcgcgggcg agcgcgagga tcgtcccggt ggcgattccc gggatggcgg
    10201 cagggaggac ctggcgcgag atcacctgcc actgcgtcga gccgagcgcg taggcgccct
    10261 ggcggatcga gtccggcacg gcgcggatcg cctcgcggct cgcgacgatg atcgtcggga
    10321 ggacgaggag cgtgaggatc atcgcgcccg cgagcagcac ccgcccgagc ccgagcccgc
    10381 gcacgaggaa ggcgagcccg aggatcccgt agacgatcga cgggacggcc gcgaggttct
    10441 ggatgttcag ctcgagcagc cggttgtacc agcggtcgcg gttcgcgtac tcctcgaggt
    10501 agatcgccgt cccgacgccg accgggacgg ccatcacgag caggaggccc atgaggtaga
    10561 tcgtcccgag gatcgccggc cgcgcgcccg ccttcagcgg gtcgaacgag ggcgggttca
    10621 ggaagagtgc ggggtcgagg aagaggacgc ccttgcgcag cacgtccacg aagagggtcc
    10681 cgaggagcac gaacccgacc gcgaccgaga agacgagaag ggcgtagaag acagcgccct
    10741 tcgcgcgctc gcgcgggtcg ccgccgcgca ggtcgaccgg ctgggcgctc attcgtagat
    10801 ctcccggtac ttgcggacga gcctgatcg
//
